LOCUS       DAUGHY010000001        94293 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.001, whole genome shotgun sequence.
ACCESSION   DAUGHY010000001 DAUGHY010000000
VERSION     DAUGHY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 94293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94293
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.001"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            16..195
                     /locus_tag="VH752_000001"
     CDS             16..195
                     /locus_tag="VH752_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEQ8288390.1"
                     /translation="MLTYDEFKQAIDHGYITGDTVAIVRKNGQIFDYVLPHEKVKNGE
                     VVTDEKVEEVLMELE"
     gene            440..976
                     /gene="rimP"
                     /locus_tag="VH752_000002"
     CDS             440..976
                     /gene="rimP"
                     /locus_tag="VH752_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEQ8288391.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIANTSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            1151..2308
                     /gene="nusA"
                     /locus_tag="VH752_000003"
     CDS             1151..2308
                     /gene="nusA"
                     /locus_tag="VH752_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEQ8288392.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATAVEEPVVDDVMTAEEV"
     gene            2324..2620
                     /locus_tag="VH752_000004"
     CDS             2324..2620
                     /locus_tag="VH752_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEQ8288393.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            2613..2915
                     /locus_tag="VH752_000005"
     CDS             2613..2915
                     /locus_tag="VH752_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEQ8288394.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAIADAGFSKKMRTLME"
     gene            2935..5796
                     /gene="infB"
                     /locus_tag="VH752_000006"
     CDS             2935..5796
                     /gene="infB"
                     /locus_tag="VH752_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEQ8288395.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIER
                     KASNVSPRHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNISAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVKNTNTAPKPVTERKFHELPKEFEYTEGMTVAEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLASLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            6002..6352
                     /gene="rbfA"
                     /locus_tag="VH752_000007"
     CDS             6002..6352
                     /gene="rbfA"
                     /locus_tag="VH752_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEQ8288396.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRELDNKS"
     gene            complement(6483..7472)
                     /locus_tag="VH752_000008"
     CDS             complement(6483..7472)
                     /locus_tag="VH752_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ8288397.1"
                     /translation="MKHPIRKTLVTLGLLLTLCLPTPVAASSRSWKSWFIEQYFWLKR
                     DKSYYSKQDDPSFQRYLDACREQSDKPYQLDTNLVNGPLVQENLYGMQVYSWNDNGKP
                     DQKTIIYLAGGSYLNNPTTYHINMLKTLSTSLDAKIVLPIYPKAPRYTYNYTMPKLVN
                     LYQHYYHKNQNIFLMGDSAGGGLALGLAHALHNESVPQPKQLVLLSPWLDVTMSHPEI
                     PNYEDADPILSSWGLKRVGELWAYSADNTNHIYVSPKNGPITYLPPITLFTGTREIFY
                     PDIRDYAAKLKAANHNITFITQEGMNHVYPIYPIEEAKTAQYQIIDAINKTPQ"
     gene            7644..8078
                     /locus_tag="VH752_000009"
     CDS             7644..8078
                     /locus_tag="VH752_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEQ8288398.1"
                     /translation="MQQISAAEWEVMRVVWASGDIKSSDIITILRKKYQWSDSTIKTL
                     IGRLVKKNLLTSYREGRAYIYQALLDETLVQKEALATVLDGICQRQHTRLLLERLYDL
                     PMTLEEIEAFQELLEVKKENAVLEVPCNCLPGQCHCQKKETY"
     gene            8078..10309
                     /locus_tag="VH752_000010"
     CDS             8078..10309
                     /locus_tag="VH752_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEQ8288399.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKTVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVKAAGYGAQVFDEHAKHMQTGRRDEDLKVIWSQVLWSALFTIP
                     ILYLAMGHMVGLWLPSFLQPDRYPFVYSFSQLLLTIPVLVLNRHYYHNGFKALFKGHP
                     NMDSLVALATSFAFAYSLYGVVEISLGQAHFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTSQAIQKLLSLKATDVRVWRNSQWQLLPLEEVSYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESLPVEKGEKDNVFAGSLNGPGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADRVSGVFVPVVLVLALLTGVFWLVFMQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGILFKGGDILEQAHQVDTVVFDKTGTVTEGKPSLQKLLI
                     FSGDDHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEQFESLTGLGVTGQLEG
                     RRLAIGNATLMAILGIDLSCVQSVCAKASGKGQNLVYYAKEGQLRALFSIADAVKEDS
                     QATVEALQQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVIADLRSQGK
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMRSDMLDLVKVMSLSRATM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            10323..10526
                     /locus_tag="VH752_000011"
     CDS             10323..10526
                     /locus_tag="VH752_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEQ8288400.1"
                     /translation="MEKHYQVTGMTCDGCVRTVTEKLSAVPGVQSVQVNLEKGQAKVT
                     GRPLTFLLKRALKDTKFELHQSS"
     gene            complement(10726..11496)
                     /locus_tag="VH752_000012"
     CDS             complement(10726..11496)
                     /locus_tag="VH752_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ8288401.1"
                     /translation="MLKRERLLKITEIVNKQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFV
                     FYHLDNIDAVVTDSQITEEVIERYSQFTQLLVAHENKE"
     gene            11733..12206
                     /locus_tag="VH752_000013"
     CDS             11733..12206
                     /locus_tag="VH752_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ8288402.1"
                     /translation="MFPNILFTEARTQPELFDLVASHLEKVGYVTQEYHQALVEREVV
                     FPTGLKVDLKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTTILSNLITFFITKGNLSYLASLKDDKQAISNYLIEKGVFQHD"
     gene            12199..12504
                     /locus_tag="VH752_000014"
     CDS             12199..12504
                     /locus_tag="VH752_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ8288403.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIESQMTDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFDLPIPIVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            12530..13981
                     /locus_tag="VH752_000015"
     CDS             12530..13981
                     /locus_tag="VH752_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEQ8288404.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAARNHLPVFVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGSQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGASAGSLITHSTLEGPMEKIFGYVIGQATTGQIASI
                     ITLIIFVAIYLGLFAWYANQMKARNAEYAKTMT"
     gene            14239..14664
                     /gene="lacA"
                     /locus_tag="VH752_000016"
     CDS             14239..14664
                     /gene="lacA"
                     /locus_tag="VH752_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ8288405.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEESYDIIDVTDINSDFIDNTL
                     AVAKAVNEAEGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     MGAEIVGPELAKNIAKGFVTGHYDGGRHQIRVDMLNKMA"
     gene            14679..15095
                     /locus_tag="VH752_000017"
     CDS             14679..15095
                     /locus_tag="VH752_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEQ8288406.1"
                     /translation="MKIAIGCDHIVTNEKMAVSDFLKSKGYDVIDCGTGVGINNAVNK
                     VPGIRSALVRDMTTALYAKEELNANVIGFGGKITGELLMCDIIDAFIKAEYKETEENK
                     KLIAKIAHLETHHASQEDPDFFTEFLERWDRGEYHD"
     gene            15105..15547
                     /locus_tag="VH752_000018"
                     /pseudo
     CDS             15105..15547
                     /locus_tag="VH752_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061423.1"
                     /note="catalyzes the formation of tagatose
                     1,6-bisphosphate from tagatose 6-phosphate and ATP;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            15594..16571
                     /gene="lacD"
                     /locus_tag="VH752_000019"
     CDS             15594..16571
                     /gene="lacD"
                     /locus_tag="VH752_000019"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ8288407.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            16768..17385
                     /locus_tag="VH752_000020"
     CDS             16768..17385
                     /locus_tag="VH752_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEQ8288408.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLIARGWVRAAVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEER"
     gene            17387..18211
                     /locus_tag="VH752_000021"
     CDS             17387..18211
                     /locus_tag="VH752_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ8288409.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLGLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPQGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALAAQKD"
     gene            complement(18247..18804)
                     /locus_tag="VH752_000022"
     CDS             complement(18247..18804)
                     /locus_tag="VH752_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ8288410.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLEIKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKMIFKHHLHHLLTRLVTKRLKAYQKLKDKKQSHYKRLFWSNAIVSV
                     LTNWISDDMIVPAEEMAAMGLPLLT"
     gene            19097..19939
                     /locus_tag="VH752_000023"
     CDS             19097..19939
                     /locus_tag="VH752_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ8288411.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTITGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLINLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYVGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            complement(20068..20790)
                     /locus_tag="VH752_000024"
     CDS             complement(20068..20790)
                     /locus_tag="VH752_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288412.1"
                     /translation="MATMKQYHKGDSMTKSQKEALYWMLSVLTITLIGGSCLIFGSYP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILP
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSDSGDKQSSKE"
     gene            20992..22623
                     /locus_tag="VH752_000025"
     CDS             20992..22623
                     /locus_tag="VH752_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEQ8288413.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKPFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADDKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            22741..23889
                     /gene="nagA"
                     /locus_tag="VH752_000026"
     CDS             22741..23889
                     /gene="nagA"
                     /locus_tag="VH752_000026"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEQ8288414.1"
                     /translation="MTCYLKADCFYYPTEVKPAGYLSLHDGVFGEWTEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEKYKGAQNPSYMKTPRLEEFDAWQKAAK
                     GLIKKIALAPERDGVKEFVSAVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVSPIACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            24011..24850
                     /locus_tag="VH752_000027"
     CDS             24011..24850
                     /locus_tag="VH752_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEQ8288415.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASLDRLGLDYV
                     DLYLIHWPNPKALRNTWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVHHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQAALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTPEDKKTIRYLSGMSAIPNPDT
                     TSF"
     gene            24982..25515
                     /locus_tag="VH752_000028"
     CDS             24982..25515
                     /locus_tag="VH752_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEQ8288416.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KDHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            25887..26804
                     /gene="glyQ"
                     /locus_tag="VH752_000029"
     CDS             25887..26804
                     /gene="glyQ"
                     /locus_tag="VH752_000029"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEQ8288417.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEISDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGLPLLDEATRAILLAEDDE"
     gene            27182..29221
                     /locus_tag="VH752_000030"
     CDS             27182..29221
                     /locus_tag="VH752_000030"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEQ8288418.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDI
                     QTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
                     TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQCVIVDAKER
                     QEMIVEQIKTLEVEQGVQVDIDEDLLNEVLNLVEFPTAFMGNFEAKYLDVPEEVLVTS
                     MKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGVV
                     LALADKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWRIPMDKLVDSLYD
                     LSFDSLTYTNKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAVAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAEDQALKNNRVAILSDLVSKAKTIAAFNQLNTK"
     gene            29375..29632
                     /locus_tag="VH752_000031"
     CDS             29375..29632
                     /locus_tag="VH752_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEQ8288419.1"
                     /translation="MDPKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            29759..30151
                     /locus_tag="VH752_000032"
     CDS             29759..30151
                     /locus_tag="VH752_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEQ8288420.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            30511..32037
                     /gene="glpK"
                     /locus_tag="VH752_000033"
     CDS             30511..32037
                     /gene="glpK"
                     /locus_tag="VH752_000033"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEQ8288421.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQFA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     IDTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDAK
                     "
     gene            32053..33891
                     /gene="glpO"
                     /locus_tag="VH752_000034"
     CDS             32053..33891
                     /gene="glpO"
                     /locus_tag="VH752_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEQ8288422.1"
                     /translation="MEFSKETRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAQGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALVASHVKAEDFLLDDNGKIIGVKARDLLS
                     DQEIIIKAKLVINTTGPWSDEIRQFSHKGQPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFNHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFDRDENGLFTLAGGKITDYRKMAEGALTGIIQI
                     LKEEFGKSFKLINSKTYPVSGGEINPANVDSEIEAYAQLGTLSGLSMDDARYLANLYG
                     SNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYEMALKPTDYFLRRTNHLLFMRDSLD
                     ALIDPVINEMAKHFEWSDQERVAQEDDLRRVIADNDLSALKGQQEG"
     gene            33893..34594
                     /locus_tag="VH752_000035"
     CDS             33893..34594
                     /locus_tag="VH752_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ8288423.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAALAVLLFQVMS"
     gene            34703..36049
                     /locus_tag="VH752_000036"
     CDS             34703..36049
                     /locus_tag="VH752_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ8288424.1"
                     /translation="MTLTTKTIHVIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESNHRFIQAEVLAIEAPSNTLLLKDSQG
                     RVFEEGYETLVCAMGASPQSHYIETSQTNKVLVTKYYEESQASLKLIEASQEVLVIGA
                     GLIGLDLAYSLSLQGKRVKLIEAAERPDFYQTDAELIAPVMAEMSTHHVTFINNKRVT
                     AIHEIEGKVVAHTEQGDTFQGDLAILAINFRPNTHLLQGQVACALDKTILVNENLQTS
                     QANIYAIGDMVSLHFGILGMDYYTPLINQAMKTGQALALHLAGYPIPPLQTVKVLGSS
                     HFGYYRASVGVTEEEAELYMDTCSYLYQNGDSKNLFWLKLIARKTDGILIGAQLLSKT
                     NALVIANQLGQALALKVTDGDLAFQDFLFLQGHSDLAYHLHEACLKLFEKRLRHED"
     gene            36039..37538
                     /locus_tag="VH752_000037"
     CDS             36039..37538
                     /locus_tag="VH752_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ8288425.1"
                     /translation="MKIEDLMDKERKAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHEHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYLEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYPLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGAILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMGQETDFSQQLSDDILALLVFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRDFQLMPYDHQKVYDCLITYQTVYLPRQDLP
                     YYRLKQYSSPYELTALEAFLKDLFQQKNTREEELSLSPAAKSSFAHKTV"
     gene            37664..38308
                     /gene="fsa"
                     /locus_tag="VH752_000038"
     CDS             37664..38308
                     /gene="fsa"
                     /locus_tag="VH752_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ8288426.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMTQHPLTTNGLKTFMEDWASFKK"
     gene            38526..40511
                     /gene="tkt"
                     /locus_tag="VH752_000039"
     CDS             38526..40511
                     /gene="tkt"
                     /locus_tag="VH752_000039"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEQ8288427.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLSVEDLKNFRQWASKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVDHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQAWNDLFAVYEAEYPELAAEYQKAF
                     TNEAAQVELEAHELGSSMASRVSSQQAIQQISEQVASFWGGSADLSASNNTMVKAETD
                     FQPGHYEGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNFNVIRPADGNETNAAWKRAIAET
                     DRPTMLVLTRQNLPVLEGTKELAEDGLNKGAYILSEAKGDLEGILIATGSEVKLAMDT
                     QEALEAEGIHVRVVSMPSQNIFDEQSAEYKESILPAAVTKRLAIEAGSSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTVANATELYKSL"
     gene            40704..40970
                     /locus_tag="VH752_000040"
     CDS             40704..40970
                     /locus_tag="VH752_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ8288428.1"
                     /translation="MEKKRQRLYDVIRQAYDYPENRENVALSQLLLAASNRLIKHSNP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASSV"
     gene            41004..41738
                     /locus_tag="VH752_000041"
     CDS             41004..41738
                     /locus_tag="VH752_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8288429.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKEMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            41743..43371
                     /locus_tag="VH752_000042"
     CDS             41743..43371
                     /locus_tag="VH752_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8288430.1"
                     /translation="MNWSTIWELIKINILYSNPQSLANLKKRQENHPKENFKAYKSMM
                     RQQALMIAMFLVIYLFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGAVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MTDGVFTDYPTIPYFKGFYDVIQAPFSTAALLNFWLPLLLILAMVYGIVTKVMPTYYR
                     EAFYISNENKAKQTKKPVNRPHQNQSLTQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGVMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLLDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     ELMYRRDYRLLDLKWQDMTQLFTRGNGQWLTMGLIFGNLIVTGVVGFGAVIIANIIQQ
                     PLLISILLSCLTLMVLGIAHLWIQKTFWKSLEKL"
     gene            43435..44256
                     /gene="proB"
                     /locus_tag="VH752_000043"
     CDS             43435..44256
                     /gene="proB"
                     /locus_tag="VH752_000043"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEQ8288431.1"
                     /translation="MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLM
                     NKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTN
                     VSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
                     LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVAVITQEIIASAGGAGSKF
                     GTGGMLSKIQSAQMVFENKGQMVLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD"
     gene            44249..45499
                     /locus_tag="VH752_000044"
     CDS             44249..45499
                     /locus_tag="VH752_000044"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEQ8288432.1"
                     /translation="MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKL
                     IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDMATNIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLE
                     KAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQ
                     HTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHA
                     RGPMDLEALTSTKFYINGDGHIRE"
     gene            45519..45740
                     /locus_tag="VH752_000045"
     CDS             45519..45740
                     /locus_tag="VH752_000045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288433.1"
                     /translation="MTNEREKEEAKVALEELLHQIQVIHLELKIVRNWGYFDLFGDGM
                     LVSWIKRRKMSKVNQLLIDLQVTLQNCRR"
     gene            45783..45947
                     /locus_tag="VH752_000046"
     CDS             45783..45947
                     /locus_tag="VH752_000046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288434.1"
                     /translation="MSWDKIFDIWLDNLLTDCCVIKEAKNFLQVVEILEAAAKSVLAK
                     LADSEQKEQD"
     gene            45971..46984
                     /gene="rsmH"
                     /locus_tag="VH752_000047"
     CDS             45971..46984
                     /gene="rsmH"
                     /locus_tag="VH752_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEQ8288435.1"
                     /translation="MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKP
                     DGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIK
                     DNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
                     AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPA
                     KELKKKGHPAKQIFQAIRIEVNDELGAADESIQDAMELLALDGRISVITFHSLEDRLT
                     KQLFKEASTVDVPKGLPLIPEDMKPKFELVSRKPILPSHSELTANKRAHSAKLRVAKK
                     IRK"
     gene            46989..47312
                     /gene="ftsL"
                     /locus_tag="VH752_000048"
     CDS             46989..47312
                     /gene="ftsL"
                     /locus_tag="VH752_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEQ8288436.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            47316..49571
                     /locus_tag="VH752_000049"
     CDS             47316..49571
                     /locus_tag="VH752_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ8288437.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGSGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            49573..50583
                     /locus_tag="VH752_000050"
     CDS             49573..50583
                     /locus_tag="VH752_000050"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEQ8288438.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLVGGLMFYFLHVSPSGISSINVFGYQLPLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQSQFDVLLLIGAMIGALLGFFC
                     FNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYVLETSSVMLQVSYFKY
                     TKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLWGVGSLASLLVLAILY
                     VF"
     gene            50741..52084
                     /locus_tag="VH752_000051"
     CDS             50741..52084
                     /locus_tag="VH752_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ8288439.1"
                     /translation="MSFKDYHFKQYVQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDEAKAEVQVVITAPSRELATQIFDACKQIAKHFQEEIRL
                     ANYVGGTDKLRQIEKLKGSQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERKAKKQHF"
     gene            52279..53082
                     /locus_tag="VH752_000052"
     CDS             52279..53082
                     /locus_tag="VH752_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ8288440.1"
                     /translation="MTSVFLTSGWAFYDYLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKFLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            53082..53825
                     /locus_tag="VH752_000053"
     CDS             53082..53825
                     /locus_tag="VH752_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ8288441.1"
                     /translation="MITIRNLSKTFSGQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQQFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            53929..54153
                     /locus_tag="VH752_000054"
     CDS             53929..54153
                     /locus_tag="VH752_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEQ8288442.1"
                     /translation="MLVEIFSLYVKGLILSSLTMILICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
     gene            54217..55134
                     /gene="trxB"
                     /locus_tag="VH752_000055"
     CDS             54217..55134
                     /gene="trxB"
                     /locus_tag="VH752_000055"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEQ8288443.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVLTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIQGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMNPVTGMVKDLEITDQEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFPS"
     gene            55303..56682
                     /locus_tag="VH752_000056"
     CDS             55303..56682
                     /locus_tag="VH752_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ8288444.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNRLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
     gene            56853..58307
                     /locus_tag="VH752_000057"
     CDS             56853..58307
                     /locus_tag="VH752_000057"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEQ8288445.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPGNFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAMIGGADATSNVRAGKRFDIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVPTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVRNFDAIPLLVDIFVKGELVYQLPTLSEIKTYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMTLIDNIRKDTHGKSE"
     gene            58309..59133
                     /gene="nadE"
                     /locus_tag="VH752_000058"
     CDS             58309..59133
                     /gene="nadE"
                     /locus_tag="VH752_000058"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEQ8288446.1"
                     /translation="MTLQEEIIRQLGVKASIDPQEEIRKTVDFLKAYLRKHSFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREETSDKAYQFIAVRLPYGVQADEADAQKALAFIA
                     PDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALGVTYQDIDDYLEGKLISKAAQATIEKWWHKGQHKRHLPITIFDDFWK"
     gene            59318..60655
                     /gene="pepC"
                     /locus_tag="VH752_000059"
     CDS             59318..60655
                     /gene="pepC"
                     /locus_tag="VH752_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEQ8288447.1"
                     /translation="MSALTETFTEQLFAHYEANAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKVENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQC"
     gene            complement(61119..63284)
                     /locus_tag="VH752_000060"
     CDS             complement(61119..63284)
                     /locus_tag="VH752_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ8288448.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLQSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQMTSDDTSSSNDISNSTNTDNNSNHPSTDDKKTTH"
     gene            complement(63271..63879)
                     /gene="recU"
                     /locus_tag="VH752_000061"
     CDS             complement(63271..63879)
                     /gene="recU"
                     /locus_tag="VH752_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEQ8288449.1"
                     /translation="MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDY
                     YLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAK
                     ETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
                     NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            63960..64475
                     /locus_tag="VH752_000062"
     CDS             63960..64475
                     /locus_tag="VH752_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEQ8288450.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLIGYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDRWNEKNQKVLSQFRAVDF
                     VKYYFPNYEQPTQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLKKAKDMPHYQLLLL
                     TFDRLNDMSQS"
     gene            64597..64923
                     /gene="gpsB"
                     /locus_tag="VH752_000063"
     CDS             64597..64923
                     /gene="gpsB"
                     /locus_tag="VH752_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEQ8288451.1"
                     /translation="MTSIIYSPKDIFEQEFKTSMRGFDKKEVDEFLDNVIKDYENFNA
                     QIEALKAENEALKKAKFQARNTVSATVQQPVPQPTRVAQSATNFDILKRISKLEKEVF
                     GKQIIE"
     gene            64937..65305
                     /gene="rnpB"
                     /locus_tag="VH752_000064"
     ncRNA           64937..65305
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VH752_000064"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            65368..66522
                     /locus_tag="VH752_000065"
     CDS             65368..66522
                     /locus_tag="VH752_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEQ8288452.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWGNYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAALIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDGRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKVNGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            66536..68005
                     /locus_tag="VH752_000066"
     CDS             66536..68005
                     /locus_tag="VH752_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288453.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERVIPEPQMTKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLAILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKATRLKAAAKAFEKQYGTFYTDATKSKLKNSAFATLPDLEAALKALEGSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDVVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQAAQAQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGYQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(68151..68633)
                     /locus_tag="VH752_000067"
     CDS             complement(68151..68633)
                     /locus_tag="VH752_000067"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEQ8288454.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGITWEDVPGTTLESCGNYKDHSLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(68707..70038)
                     /locus_tag="VH752_000068"
                     /pseudo
     CDS             complement(68707..70038)
                     /locus_tag="VH752_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
     gene            complement(70109..70867)
                     /locus_tag="VH752_000069"
     CDS             complement(70109..70867)
                     /locus_tag="VH752_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEQ8288455.1"
                     /translation="MHLSLVLQAELENNRFTFLKEGKTVVFSDINQEKLDQVVADYTK
                     EGYDAFSVVCDVTKEVAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFPTEKFEFM
                     IKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHGLIGLTKV
                     TALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPLVPQKRLI
                     DVQEIADYVSFLASDKAKGVTGQACILDGGYTAQ"
     gene            complement(70919..71578)
                     /locus_tag="VH752_000070"
     CDS             complement(70919..71578)
                     /locus_tag="VH752_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ8288456.1"
                     /translation="MGTSATVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPEGVTITLQSENGFVGLTGLTDDHYDPSIVNAGGQPVSIAPGGAFFDSSTSFGIIRG
                     GHVAATVLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKAK
                     ILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINPDYTFEDVQNVTEVTLIDKT
                     N"
     gene            complement(71580..72230)
                     /locus_tag="VH752_000071"
     CDS             complement(71580..72230)
                     /locus_tag="VH752_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEQ8288457.1"
                     /translation="MCKEITIKEAVAHVKDGDTIMVGGFMTNGTPEKLIDALVEKGVK
                     DLTLICNDAGFPDKGVGKMVANKQFSTIIASHIGLNREAGRQMTEGETVIDLVPQGTL
                     AERIRSGGFGLGGFLTPTGIGTEVAKGKEVITIDGKDYLLEKPLKADIALIFANKADK
                     NGNLQYAGSENNFNHVMAANAKTTIVEAREVVDVGQMDPNFVHTPGIFVNYLVKGA"
     gene            complement(72254..73441)
                     /locus_tag="VH752_000072"
     CDS             complement(72254..73441)
                     /locus_tag="VH752_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ8288458.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQVAVHSGIPEDKTAFTLDMVCGSGLKAIQLAAQSI
                     MLGDADIVIAGGVENMSQAAYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAARALENHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGTGPIPATQKALKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
     gene            73642..74538
                     /locus_tag="VH752_000073"
     CDS             73642..74538
                     /locus_tag="VH752_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ8288459.1"
                     /translation="MDIKQIRYFIAIVENHFNLSQAAELLYVSQPTLSMMINDFEKRE
                     NVKLFKRKRGRIIGLTYLGDNYYKDAQKVLSLYDDMFLKLHDHSKGLKGSINIGIPPL
                     ILSVVFSEVMSKLILENPGIQFNVKEIGAYQLKNELLVGNVDVAVLLSPTGIADNLVE
                     TYEIQRSELSVCLSPRHRLASKKVIQWKDLTDEQLALFDPSFMVHHLVLEACERHQVR
                     PNIILTSSSWDFMLNSTKINHNVLTICPKPITELYQLKDIKCIPMERPISWRVVLTRL
                     RKKSYSEIEAYIMDDLLQSFFK"
     gene            74668..76275
                     /locus_tag="VH752_000074"
     CDS             74668..76275
                     /locus_tag="VH752_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEQ8288460.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            76385..77020
                     /gene="gmk"
                     /locus_tag="VH752_000075"
     CDS             76385..77020
                     /gene="gmk"
                     /locus_tag="VH752_000075"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEQ8288461.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKNPFKAK"
     gene            77036..77353
                     /locus_tag="VH752_000076"
     CDS             77036..77353
                     /locus_tag="VH752_000076"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEQ8288462.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            77418..79802
                     /locus_tag="VH752_000077"
     CDS             77418..79802
                     /locus_tag="VH752_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEQ8288463.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFVIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKAIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVAYFVTNHLIRIDERQIDRSEPYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKIKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFY
                     RYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSHLSDNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            79864..80799
                     /locus_tag="VH752_000078"
     CDS             79864..80799
                     /locus_tag="VH752_000078"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEQ8288464.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLGADGIITAAFGQFLPTLLLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSN
                     QEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVIVKTKKSLVIATGQGA
                     LSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR"
     gene            80789..82111
                     /gene="rsmB"
                     /locus_tag="VH752_000079"
     CDS             80789..82111
                     /gene="rsmB"
                     /locus_tag="VH752_000079"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEQ8288465.1"
                     /translation="MADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSNKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLTYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSHRAVAIMESLFVRSKASIRVTDPLKLEEVAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADIVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            82149..82889
                     /locus_tag="VH752_000080"
     CDS             82149..82889
                     /locus_tag="VH752_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEQ8288466.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGVRVLEPGDYLVINSDGLTNMISNDEIVTILGSKVSLDEKNQ
                     EMIDLANLRGGLDNITIALVHNESEDVE"
     gene            82886..84784
                     /gene="pknB"
                     /locus_tag="VH752_000081"
     CDS             82886..84784
                     /gene="pknB"
                     /locus_tag="VH752_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEQ8288467.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSRERKIIFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTDALQPPT
                     KKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDV
                     GLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDY
                     QEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLSVAV
                     SDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAIVSG
                     QSPYYGTSLSLSDKGEISLYLYPEVTHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSP
                     SETTAQTP"
     gene            84907..85599
                     /locus_tag="VH752_000082"
     CDS             84907..85599
                     /locus_tag="VH752_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEQ8288468.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDITVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            85596..86600
                     /locus_tag="VH752_000083"
     CDS             85596..86600
                     /locus_tag="VH752_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8288469.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
                     SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLNMILKELTD
                     KSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKM
                     IDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
                     "
     gene            86593..87234
                     /locus_tag="VH752_000084"
     CDS             86593..87234
                     /locus_tag="VH752_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8288470.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVDDRTQAVVYAFRHHLVPQDDN"
     gene            87271..88671
                     /locus_tag="VH752_000085"
     CDS             87271..88671
                     /locus_tag="VH752_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ8288471.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPRLQEKADVITKKVEEDGILYALEELGLIDKELQFPQL
                     DLENHTGPKVTIKTNHGDMTLVLFPEHSPKTVANFLGLAKEGYYDGIIFHRVIPEFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQFFIVQNSKIP
                     YAKKELERGGWPTPIAAAYAENGGTPHLDRRHTVFGQLADDASFKVLDLIASVETGAQ
                     DKPKEDVIIETIEVFD"
     gene            88671..89048
                     /locus_tag="VH752_000086"
     CDS             88671..89048
                     /locus_tag="VH752_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ8288472.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALDNGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKASLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
     gene            complement(89066..90007)
                     /gene="cysK"
                     /locus_tag="VH752_000087"
     CDS             complement(89066..90007)
                     /gene="cysK"
                     /locus_tag="VH752_000087"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEQ8288473.1"
                     /translation="MTKIYKTITELVGQTPIIKLNCLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGLISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKANPETAIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARLTGAKEGFLVGISSGAALYAAI
                     EVAKQLGKGKHVLTILPDNGERYLSTELYDVPVIKTK"
     gene            complement(90135..90767)
                     /locus_tag="VH752_000088"
     CDS             complement(90135..90767)
                     /locus_tag="VH752_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEQ8288474.1"
                     /translation="MEHFKTIKASGFFEESIKKSRFICHIKRVSTEEDGKNFVNAIKK
                     EHYKANHSCFAMIIGNNSQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFG
                     GIKLGTGGLIRAYSNMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTE
                     TETKFSDTIKTTLYCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
     gene            90822..92147
                     /locus_tag="VH752_000089"
     CDS             90822..92147
                     /locus_tag="VH752_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ8288475.1"
                     /translation="MESIENSYGRLLLENQLPDSARQLAQPLKSVVISRGKMICQRCH
                     YQLDEEARLPSGAYYCRFCLVFGRNQSNKLLYAIPPMHFPKGNYLVWSGQLTAYQEMI
                     SQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
                     LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQL
                     HYAASQALKEGGAKILLTATSTRTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFN
                     LFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAILKELLPAEQIACVS
                     SQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAG
                     RVGRSIDRPDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN"
     gene            92119..92784
                     /locus_tag="VH752_000090"
     CDS             92119..92784
                     /locus_tag="VH752_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEQ8288476.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACRDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVSPGCFRERQFNQVSAILEAANVSYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            92864..93412
                     /gene="raiA"
                     /locus_tag="VH752_000091"
     CDS             92864..93412
                     /gene="raiA"
                     /locus_tag="VH752_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEQ8288477.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDGATNILYRREDGNLGLIEAK"
BASE COUNT        29305 a        16810 c        20064 g        28114 t
ORIGIN      
        1 ataaggaggt aatctatgct aacatacgac gaatttaagc aagcaatcga ccatgggtat
       61 atcacaggag acacagtagc gattgtgcgc aaaaacggac agatctttga ttatgtgttg
      121 ccacatgaga aagtaaagaa tggagaagtt gtgacagatg aaaaagtgga agaagtgttg
      181 atggaattgg agtaacatta cccgtcaatc acccgtttat ttttattttt tcatcttttt
      241 ctatcctttc tatattttaa aaataccaac gtttaagtgt cttgcaaagg atgaaaacgg
      301 tgagatagct accagcaatc ccctcctctc cttgtgagta aaacaaagtg catatccttg
      361 cattcctctt aaattatggt ataataggct taaataatac agaaaggtgg tgggatatgt
      421 ctcgccactt ttgtattaag tggactccca aggaccaatt atcttagaga agagcataaa
      481 aatagaggag gtaatcaaaa tagcaaatac atctattatc gatatcgtaa ccaagacagt
      541 aacacctgag attaaggcac cttatgaatt agtagacgtt gaatatgaca aaatgggtag
      601 tgactacatc cttagtattt tagtcgataa ggaaggtgga atcacagtgg aagatacatc
      661 tgacttgacg aatattatta gtccgctgtt agatacgatt gatccagatc cttttccaaa
      721 ccaatatatg ctagaagtct ctagtccagg tttggagcgt ccgcttaaga cagctgacag
      781 tctaaaagca gcagttggct cttatatcaa tgtgagtctt tatcaagcta ttgataaggt
      841 gaaagttttt caaggagact tattagcctt tgatggtgaa acgttgacca ttgattatct
      901 tgataaaaca cgtcataaga tcgtgaatat accatatcaa gcagttgcta aggtgagaat
      961 ggctgtcaaa ttataacttt tatttcaaag cactaaactt ttttagaaac acttaagcaa
     1021 atttgaaaca acacctatag agtttataca agctaaacaa aaggaattaa gggagccttt
     1081 agggggctat tgatcgtatt aatttaagaa aggatttttg ccaagcttta caagcaaggt
     1141 agataacatt atgagcaaag aaatgctaga agccttccgt attttggaag aagaaaaaca
     1201 cattgataaa gcagatatta ttgacgcagt gacagaatca ttaaagtctg cctataaacg
     1261 tcgctatggt caatcagaat catgtgttat tgaatttaat gaaaaaacgg ctgattttca
     1321 agtattcacg gttcgtgagg ttgttgaaga agtttttgac agccggcttg agattagttt
     1381 gaaagatgct cttgctatca gttctgccta tgaattgggt gataagattc gctttgaaga
     1441 atctgtcaat gaatttggac gtgtcgcagc tcaatcagct aaacagacta ttatggaaaa
     1501 gatgcgccgc caaatgcgtg aagtgatgtt caatgaatac aaagagcatg aaggtgaaat
     1561 tatgacagga acagttgaac ggtttgatca acgttttatt tatgtcaatc ttggttcctt
     1621 agaagcacaa ttgtctcacc aagaccagat tccaggtgaa actttcaaat cacatgaccg
     1681 tattgacgtt tatgtctaca aagttgaaaa taatcctaaa ggagttaacg tctttgttag
     1741 tcgtagccat ccagaattca tcaaacgtat tatggagcaa gaaattcctg aagtctttga
     1801 tggtactgtt gaaatcatga gtgtttctcg tgaagcaggt gaccgcacca aggttgctgt
     1861 gcgcagtcat aatcctaacg ttgatgccat cggaacaatt gttggacgtg gtggaagtaa
     1921 tattaaaaag gtcatcagca agttccatcc aaaacgtgta gatgctaaga cggggcttga
     1981 aattccagtt gaagaaaata ttgacgtgat tcaatgggta gatgatccag ctgagttcat
     2041 ctacaatgcc attgctccgg ctgaagtaga catggtactt tttgacgatg aagatcttaa
     2101 acgtgctacg gtagtagttc ctgatagcaa attatcatta gctatcgggc gtcgcggcca
     2161 aaatgttcgt ttagcggcac atttgactgg ctaccgtatt gatatcaaat cagctagcga
     2221 atatgatcgt cttgaggcgg aaaaagaagc agcaacagct gtggaagagc cagttgttga
     2281 cgatgttatg actgccgagg aagtataaga aaagaggtgt ttgatgtcta aagtcaaaaa
     2341 aataccttta cgaaaatcac ttgtttctgg tgaaattatt gctaaacgag atcttcttag
     2401 gattgtaaaa actaaggatg gtcaagtttt cattgatcca actggtaaac aaaatggtcg
     2461 tggtgcttat atcaaattag acaatcaaga ggccttaatg gccaagaaaa aacaagtttt
     2521 caacaggagt ttctcaatgg acattccaga aagtttttac gatgacttga ttgcttatgt
     2581 tgatcacaaa atcaaaagaa gagagttagg tcttgactaa tttagaaaga ttatctagtc
     2641 ttattggtct agctcaacgg gctggaaaag tgatttcagg tgaagaacta gtagtaaaag
     2701 ctattcaaca ccagcaggta atattagtat ttttagccaa tgatgctggt cctaatgtga
     2761 caaaaaaagt gacagataaa agtaattatt ataatgtaga agtctccaca gtgttgaatg
     2821 cactggaatt aagtgctgca ctaggtaaac caaggaaggt tgctgccatt gcagatgctg
     2881 gattttcaaa gaaaatgagg actcttatgg aatagatgaa taggaggaca caaattgtca
     2941 aagaaaagat tacatgaaat tgccaaggaa attggtaaaa gtagtaaaga ggtggttgaa
     3001 cacgctaaat acttaggatt agatgtgaaa agccatgcat caagtgttga agaagcggat
     3061 gcgaaaaaga ttatttcaag tttttcaaag gcttctaaac ctgatgtgac agcttctcaa
     3121 acagtcaaac ccaaagaagt tgctcaaccc tctgtaacag tggtcaaaga aacaggttct
     3181 gagcatgtag agaaaacaca ggtctctaag ccaaagagtc gtaattttaa agctgaaaga
     3241 gaggctcgtg caaaggaaca ggctgctcga aaacaagcaa atggctcttc tcatcgttct
     3301 caagaacgtc gaggaggtta tcgccagccc aataatcatc aaactaacga acaaggagat
     3361 aagaggatta ctcatcgttc tcaaggtgat acaaacgata agcgtattga gcgaaaagca
     3421 tcgaatgtat cacctagaca tgataatcat cagctagttg gtgatcgtaa ccgttccttt
     3481 gctaaggaga atcacaagaa tggtcgattc acaaaccaaa aaaagcaagg acgacaagag
     3541 ccccaatcga aaagtcctaa aattgatttc aaagctcgtg ctgctgcctt aaaagctgaa
     3601 caaaatgctg aatactcacg ccaaagtgaa acacgttttc gtgcacagca agaagctaag
     3661 cgtttagctg agttggcaag acaagaagct aaagaagcgg ctctaaaagc acaagctgag
     3721 gagatgagcc atcgtgaagc agcactgaaa tctattgaag aagcagaaac taaattaaag
     3781 tcatcaaata ttagtgctaa atctactgct gataatcgcc gtaaaaaaca agcacgtcct
     3841 gaaaagaatc gtgagttaac tcatcattca caagaaggac aaaagaaaaa taagaagagt
     3901 tggaatagtc aaaaccaagt gagaaatcaa aaaaatagta actggaataa aaataaaaag
     3961 accaaaaaag gcaaaaatgt taagaatacc aacaccgctc caaaaccagt aaccgagcgt
     4021 aaattccatg aattgccaaa agaatttgaa tatactgaag gcatgacagt tgccgaaatc
     4081 gcaaaacgta tcaaacgcga acccgctgaa attgttaaaa agctgtttat gatgggtgtt
     4141 atggcaactc aaaaccagtc tttagatggg gatactatcg aattattgat ggtagattat
     4201 ggtattgagg ctaaggctaa ggtagaagta gatgatgctg atatcgagcg cttctttgag
     4261 gatgaaaatt acctcaatcc tgaaaatatt gtagaacgcg cacctgttgt aactatcatg
     4321 ggacacgtag accacggtaa gacaactttg cttgatactt tgcgtaactc acgtgtggca
     4381 acaggagaag ctggaggaat tactcagcat attggtgctt accagattga agaagctggc
     4441 aagaaaatta ccttcttgga tactcccgga cacgcggcct ttacaagcat gcgcgcacgt
     4501 ggagcttccg ttaccgatat caccattttg attgttgcag ctgatgatgg tgttatgcca
     4561 caaacgattg aagcgatcaa tcactcaaaa gctgctggtg ttccaattat tgttgctatc
     4621 aacaaaattg ataaaccagg tgctaatcca gaacgtgtta tcgcagaatt ggcagaatat
     4681 ggtatcatct caactgcttg gggcggagag tgcgaatttg tagagatatc tgctaaattt
     4741 aataaaaata ttgatgaact attagaaaca gtccttttgg ttgctgaagt ggaagaattg
     4801 aaagctgatc cgacggttcg tgctattggt actgtaattg aagcacgctt agataaaggt
     4861 aaaggtgcta tcgctacgct tcttgtccaa caaggaactc ttcatgttca agacccaatc
     4921 gttgttggta atacctttgg tcgtgtccgt gccatggtaa atgaccttgg gcgtcgtgtt
     4981 aaatctgcgg agccatcaac accagtttcc attactggtt tgaacgaaac accaatggct
     5041 ggtgatcact ttgctgttta cgctgatgaa aaagctgcgc gtgcagctgg tgaggagcga
     5101 tcaaaacgtg ccctcctcaa acaacgtcaa aatacacagc gtgttagcct tgataatctc
     5161 tttgataccc ttaaagcagg agaaatcaag actgttaatg tcatcatcaa agcagacgtt
     5221 caaggttctg ttgaagccct tgcagcatct cttgtaaaga ttgaagtgga aggtgtccgt
     5281 gtcaatgtcg ttcactctgc tgtaggtgct atcaatgagt ctgatgtgac ccttgctgaa
     5341 gcatcaaatg ctgttattat cggtttcaac gttcgcccta ccccacaagc tcgtcaacaa
     5401 gcagatacgg atgatgtgga aattcgtctt catagtatta tctataaagt cattgaagaa
     5461 gtagaagaag ccatgaaagg gaaacttgac cctgtttacc aagaaaaaat tcttggtgaa
     5521 gccattattc gtgaaacctt caaagtttct aaagtgggaa caattggtgg ctttatggta
     5581 atcaacggta aggtgacacg tgattcgagt gttcgtgtta tccgtgatag tgtcgttatt
     5641 tttgatggta aactggccag cttgaaacat tacaaagatg acgttaaaga agtcggtaat
     5701 gctcaagaag gtgggttgat gattgaaaac tttaacgatc ttaaagttga cgatactatt
     5761 gaagcctata ttatggaaga aattgtgaga aaataggggc taagaaggtc tgagatgtaa
     5821 cgtttttcct ctgtcggaaa taatgacttt ttctaccaga tgagctactt agggcattcc
     5881 ttccagtctc gtcatggaat tctcagattc cctttaattg tcgttatcaa aacaggtagc
     5941 aatgtcataa gctgagcttt cagcctagct tttgttttaa aaatgtggaa aggaaatggc
     6001 tatggctaat catcgtattg accgcgttgg gatggaaatc aagcgcgaag taaatgatat
     6061 tcttcagaaa aaagtgcgtg atccacgcgt tcagggagta acgattacag aagtgcaaat
     6121 gcagggtgac ttgtcacttg ctaaagttta ttacactatt atgagtgact tggcatctga
     6181 taatcaaaaa gcacaaacag ggcttgaaaa agcgactgga acgattaaac gtgaacttgg
     6241 caaacaattg accatgtaca aaattccaga tttggtgttt gaaaaagaca actccatcgc
     6301 ctatggtaat aaaattgatc aactcttacg agaacttgac aacaaatcct aagtattgcg
     6361 gtgcggatgt tatgacagaa agagccttat cattatgata gggctctttt taattgttat
     6421 tttgtggtgc aaactacaaa aaaaacacca gattaactgg tggcaggtag gaattattcc
     6481 cctcattgag gagttttgtt gatggcatca atgatttggt attgagctgt ttttgcctct
     6541 tcgataggat aaattgggta aacgtggttc atgccttctt gggtaatgaa ggtaatattg
     6601 tggttagcgg ctttcagttt tgcggcataa tcgcgaatat caggataaaa gatctctcgt
     6661 gtcccagtaa ataaagtaat tggtggtaga taagtgatcg gaccattttt aggactgaca
     6721 taaatatggt tggtattatc agcagagtag gcccacaatt ctcccactcg ttttaatccc
     6781 caagaagata gaataggatc agcatcttcg tagtttggaa tctctgggtg tgacatagta
     6841 acatctaacc aaggtgataa aagaacgagc tgctttggtt gagggactga ttcattgtgt
     6901 agggcatgag ctagacctaa ggctaaacct ccacctgctg aatctcccat aaggaaaata
     6961 ttctgatttt tatggtaata gtgttggtaa agattgacca attttggcat agtatagtta
     7021 taggtataac gtggggcttt aggataaatg ggtaaaacaa ttttagcgtc aagacttgtg
     7081 gataatgtct ttaacatatt aatatggtat gtcgttggat tgttaagata agaaccaccg
     7141 gcaagataaa taattgtttt ttgatcaggt tttccattat cattccaaga gtaaacctgc
     7201 atgccataaa gattttcttg aacaagggga ccattgacta aattagtatc tagttgataa
     7261 ggtttatcag attgttcacg acaggcatca aggtatcttt ggaagcttgg gtcatcttgt
     7321 ttagaatagt aactcttgtc acgttttaac cagaaatatt gctcgataaa ccagctcttc
     7381 caagaacgag aactagctgc aactggagtc ggcaggcata aggtcagtaa tagacctaag
     7441 gtgacaagtg tttttcgaat aggatgtttc aagggtatct ccttaatgta aatgttattt
     7501 ttattgcaat aatattatag taaaaacgat tcatttgtca atataaggat agaaaaatga
     7561 gaaaagttat ttatttacaa atgtagttaa attcgttata attaatttac aaacgtaaat
     7621 atattgtgaa tgaaggtgac gttatgcaac agatatcagc tgccgaatgg gaagtcatgc
     7681 gtgtggtgtg ggccagtggt gatattaaaa gtagcgatat tattactatt ttaaggaaaa
     7741 aatatcaatg gtcagattcc actattaaga ccctcattgg taggctagtt aaaaaaaatc
     7801 ttttaacatc ttatcgggaa ggtagggcct atatttatca agctcttttg gatgagactt
     7861 tggtacaaaa agaagcacta gcgactgttt tagacggtat ttgtcagcgg caacacacgc
     7921 gtctacttct tgaacggctt tatgacttac cgatgacctt agaggaaatt gaggcattcc
     7981 aagagctact agaagtcaaa aaagaaaatg cagttcttga ggtgccgtgt aattgcctgc
     8041 ctgggcagtg ccactgtcag aaaaaggaga cgtattaatg gctaaagaga tatttttagt
     8101 ggagggaatg agttgtgcct cttgtgctct aacaattgaa aaaacggtca atcaattgcc
     8161 tgaagtagaa caggctgttg tgaatttagc gaccgagaaa ctcacggtta cttatcaaga
     8221 tgacgattta gaaacggcca aagttattca agcggtaaaa gctgctggtt atggtgcaca
     8281 agtctttgat gaacatgcaa aacacatgca aacaggtcgt cgtgacgaag acctaaaggt
     8341 tatttggtct caggtcttgt ggtctgccct attcaccatt cctatattat acctagctat
     8401 gggtcacatg gtgggcttat ggcttccttc atttctgcaa ccagatcgct atcctttcgt
     8461 ttatagcttt agtcagctac tcttgacgat tccagtctta gtattaaatc gtcattatta
     8521 ccataatggg ttcaaagctt tgttcaaggg acatcctaat atggactcct tggtagcttt
     8581 agcaaccagc tttgcttttg cttatagtct ttatggagtg gttgaaatca gtttgggcca
     8641 ggcgcacttt gtgcactccc tttattttga atcggtggta gtgattttga cactcatcgg
     8701 tctaggaaag tattttgaaa gtcgttctaa gggccgtact tctcaagcta ttcaaaagct
     8761 cctatccttg aaggctactg atgtgagggt atggagaaac agccaatggc aattgttacc
     8821 attagaagaa gttagctatg atgaccttgt cctggttcaa ccaggtgaaa aagtttctat
     8881 tgacggtatt atcgtggaag gacatagcag tttggatgaa tcctttttaa ctggggaaag
     8941 tttgcctgtt gaaaagggag aaaaagataa tgtctttgcg ggcagcttga atgggccagg
     9001 cgccctcacg attaaaccag ataagcttgg gaatgatacc ttgttagcac aaattatcca
     9061 actagttgag aatgctcaag aaaataaagc acctattgca gctattgctg atagagtctc
     9121 aggtgtcttt gttccggtgg ttttggtatt agctctgtta acaggggtat tttggttggt
     9181 attcatgcag gaaagtcttc gttttagttt gacaacggcc atcgctgttc tagtcattgc
     9241 ttgtccttgt gcgcttggtt tagcaactcc gacagcgatt atggttggaa cagggcgtgc
     9301 agctgaaaat ggtattcttt ttaaaggtgg tgacatctta gaacaagctc atcaggtaga
     9361 tacggttgtt tttgataaaa cggggacggt tacggagggg aaaccaagtc ttcaaaagct
     9421 cctaattttt tcgggagatg atcaccttat cttgcaagaa gctgcttcct tggaggccta
     9481 ttctcaacac cccttagggg aggctattgt tcgtgctgct caggaagctg gttacgatag
     9541 tctaccagtg gaacagtttg agtctttaac cggcctagga gtcactggtc agcttgaagg
     9601 gcgtcgtttg gcgattggaa atgctacttt aatggcaatt cttggtatcg atttatcttg
     9661 tgttcagtca gtttgtgcta aggcttcagg aaaaggccaa aaccttgtat attatgctaa
     9721 agaggggcag ctacgagctc ttttcagtat tgctgacgct gtcaaagaag atagtcaagc
     9781 taccgtcgaa gctttgcagc aactggggat tcacacgatc atgctaacag gtgaccacga
     9841 tgctaccgct aaggctattg cctctcaagt ggggattaca gatgtgatta gtcaagtctt
     9901 acctgatcaa aaagcaggcg tgatagcaga cctccgctct caagggaaaa aagttgctat
     9961 ggtaggtgat ggtattaatg atgctccggc attggccgta gcagacatcg ggatagctat
    10021 ggggtcgggg acagatattg cgattgaatc ggcggatgtg attctgatga ggtctgatat
    10081 gctggatttg gtgaaagtca tgtcattgag tcgagcgact atgaggattg tcaaggaaaa
    10141 tttattttgg gcattcatat acaatgtcct catgattcca gtggccatgg gcttacttca
    10201 tctttttgga ggcccattat tgaacccgat gttagcaggt tttgccatga gttttagttc
    10261 agtatcagtt gttttgaatg cattgcgttt aaaaggaaaa accatttaaa ggagaataca
    10321 aaatggaaaa acattatcag gttacaggaa tgacatgtga cggttgcgta agaacggtca
    10381 ctgaaaaatt atcagctgtc cccggtgtgc aatcggtcca ggtaaatttg gaaaaaggac
    10441 aggctaaggt gacaggacgt cctttaacgt tcctactcaa gcgtgctttg aaggatacca
    10501 aatttgaatt acatcaatca tcttaggtta ttttggtagc cttacttcat ctcaaaagag
    10561 gttccagttt aaactgggcc tcttttttat ggtcgctaat aaagactctg acgttagcag
    10621 gtgtgttccg agcaaaataa aaacacctct ctggaagcaa atcacttttg ccagaaggtg
    10681 ccgagggacg atgcgctgac aggctgtcca ttcttgatga aacgtttact ccttgttttc
    10741 gtgggctact aataattggg tgaactgact ataacgttca atgacttcct cagttatttg
    10801 agaatccgta acaactgcat caatgttatc gagatggtag aaaacgaaaa agtcataggc
    10861 attgaatttt tgattgtcta ccaagaggta tttttcgata gagttgttga aggctagttt
    10921 ttgaatctct ccttcagttt cgctataagt tgcaatatcg tttttataaa tgccattaca
    10981 gctaataaaa gccttggcaa attttaagga tgagatattt tgactagcaa gggaccctac
    11041 aaaagctcct gtaatagatc ggtattcacc accgataaga atcaggtcaa tggtttcgct
    11101 gtcctgaagg atgttaaaga cagggaggct attggtcaca atgcgaatct gacgattttt
    11161 taattgctca gcgaaacatt ctaaagttgt tcctggacca ataaagattg tttcaccatc
    11221 attgactagt tgtgtcgcat atgaagctag ttcccatttt tcctttgttt gaacggtttg
    11281 tttttcaata tttgaccgct cttttttatt aggcatggta atactttgtg cacctccatg
    11341 gatgcggatc aatttgccgg ctttttccag ttcatctaag tctctacgaa ctgtcatatc
    11401 agaaacgttt aaagtctgaa taatgtcatt cacagtaaca ataccttgct tattgactat
    11461 ttcggtaatt tttagaagtc tttctctttt taacatctaa ggggctcctt tctatagttt
    11521 atttacttat gtttatcata gcatatagac cttcatattt caataaaaca aaataaacaa
    11581 aacaaaaaca aacatataga acaaaaaata ttgcgtaaaa atgttgaatt atgttgaagg
    11641 ttatactata atgattttgt aaacggttac caatttgttg atgatgagat gacagttgtt
    11701 aaaaattgat acttatagaa aggcggtagg gagtgtttcc taatattcta tttactgaag
    11761 caaggacgca gccagagctt tttgatcttg ttgctagcca cttggaaaag gttgggtatg
    11821 tgacgcaaga gtaccatcag gctttagtgg agcgtgaggt ggtatttccg acaggactca
    11881 aagtggacct caaagatggg tcagatattt tgtatgcggc tattccgcat acagaaacca
    11941 agtattgctt ggttgatcaa gtggtctatg ttagaaatag tcaagccctt acctttaagc
    12001 acatgattaa tccagaggaa gattgtttgg taacggattt cttctttatt atcaatagtc
    12061 aaaacgaggg tcaaacgacg attctatcca atctcattac cttctttatt acgaaaggca
    12121 atctgagcta tttagccagt ctaaaagatg ataaacaagc tatttcaaac tatttaatag
    12181 aaaaaggagt gtttcagcat gattaaaatt ctagcagcct gcggtgcagg tgttaactca
    12241 agccatcaaa ttaaagatgc tatcgaaagc caaatgactg atcgtggcta tgatgtgcat
    12301 tgtgatgcgg tgatggtcaa agacatcaca gaagaattgg tgtctcgtta tgatattttt
    12361 acgccgattg caaaaactga cttgggattt gacctgccga ttcccatagt agaagcagga
    12421 ccgattctct atcgtatccc aatcatgagt gagcctgttt ttgctgaatt agagcgcgtg
    12481 attaaagaaa accatttgca gtgagttaat aaaaacataa ggagacaaga tgatgcaacc
    12541 ttttttagac attattaaca agattctggg atttcctatt cagttgggat caggtgtggt
    12601 catgctgatt gtcatgacag gtttagccat gattttcggg gttaaattca caaaggcact
    12661 ggaaggcggg attaagttag ctatcgctct tacaggtatt ggagccatta ttgggatttt
    12721 gacgggggct ttttcagagt ctttacaggc ctttgttaaa aatacaggga tcagtttaaa
    12781 cattattgat gtcggttggg caccgcttgc aacaattacc tggggttccc cttacaccct
    12841 ttatttcttg ctggtgatgc tggtggttaa tattgtcatg attgtcatga aaaaaacgga
    12901 cacccttgac gtcgatattt ttgatatttg gcatttatct atcacaggtc tattgattat
    12961 gtggtatgcg gctcgcaacc atttaccagt ctttgtgtct cttttgattg ctactgtggc
    13021 agttattctt gttggggtat tgaaaatcat taactctgat ttgatgaaac cgacctttga
    13081 tgaccttctt gggacagggt ctcaatcacc aatgacttca actcacatga actacatgat
    13141 gaatccaatc attatggtct tagataagat ttttgacaag gtctttccgg ggctagataa
    13201 atatgacttt gatgctgcta aattgaacaa aaaaattggc ttctggggct ctaagttctt
    13261 tatcggaatg gctctaggtt ttgtgattgg gattatggga gatcctcatt tcactgttga
    13321 atctatcaaa aattggtttg gtctaggctt taccgcgggt gcctgtttgg aacttttctc
    13381 tttgattggt tcttggttta tcgcagctgt tgagcctttg tcacaaggga ttactaattt
    13441 tgccaatgct agaatgcatg gacgtcgttt caatatcggt ttggactggc ctttcattgc
    13501 tggtcgcgct gaaatctggg cctgtgccaa tattcttgcc ccaatcatgt tgattgaagc
    13561 tgttcttctt tcaaaagttg gcaatgggat tttaccattg gcgggaatta ttgctatggg
    13621 gatgacacca gctcttcttg tggtcacacg tggccgtttg attcgcatga ttatttttgg
    13681 ctccctctta ttgccgttat tcctcttgtc aggaaccatg attgccccat tcgcaactga
    13741 attggctaaa aaagtaggcg ctttcccagc aggtgcaagt gctggttcct tgattacgca
    13801 ttcgactctc gaaggaccaa tggaaaaaat ctttggttat gtgattggtc aagcgacaac
    13861 aggtcagatt gcctcaatca ttacgttaat catctttgtg gccatttacc taggtctctt
    13921 tgcttggtat gctaaccaaa tgaaggcacg taacgctgag tatgcgaaaa caatgacata
    13981 aaatcaggtt aggtgactaa ctaaaaaact attgggaata gcagataacc gcttaaggta
    14041 ttcctgcttt atccccctag agagaaaaga gtattcctca tgtaaggcaa ggcgctctgt
    14101 gggtacccgc aagcgctttt cttgaaagaa ataatggaag acaactaaga ctgctggcaa
    14161 taatcctgac ctaaggacag gtaatcctgt tacctttttg tagattggta agcagtttgg
    14221 attagaaaag gagaaacgat ggctattatt ttaggtgcag atgcacacgg aaatgcatta
    14281 aaagaattaa tcaaaagttt tcttcaagaa gaaagctatg acattattga cgtgactgat
    14341 attaacagtg attttattga taacacatta gcggtggcta aagccgttaa tgaagcagaa
    14401 ggtcgtttag gcattatggt ggatgcttac ggagcaggtc cctttatggt agccacaaaa
    14461 ctaaaaggaa tggttgctgc tgaagtttca gatgaacgct ctgcttacat gactcgcggt
    14521 cacaacaatg cccgtatgat taccatggga gcagaaattg taggtccaga attagcgaag
    14581 aatatcgcca aaggttttgt gactggccac tacgatggtg gtcgtcatca aattcgtgtt
    14641 gacatgctca ataaaatggc ttaaaggagg ataaagagat gaaaattgcg attggatgtg
    14701 accatattgt caccaacgaa aaaatggctg tttctgattt tttgaaatct aaaggctatg
    14761 acgttattga ctgtggcact ggagttggca ttaacaatgc ggtaaataag gtaccaggta
    14821 ttcgttcagc ccttgttcga gatatgacaa cagcgcttta tgccaaggaa gaattgaatg
    14881 ccaatgtgat tggctttggt ggtaaaatca cgggtgaact tcttatgtgt gatattatag
    14941 atgcttttat caaggcagaa tacaaagaaa ctgaagagaa taagaagcta attgctaaaa
    15001 tagcccactt ggaaactcat catgccagcc aagaagatcc tgatttcttt acagaattct
    15061 tagaaagatg ggatcgtggc gagtatcacg attaggaaga agagatgagt ttaacagtta
    15121 tcttaaatcc ttctgtcgct atttcctatc ctttagattg tctggctatg gacacgggac
    15181 agaacggcaa aaacagctgg cgataaggag ctaaatgtga ccagaatgtt agcaaaagct
    15241 ggtcagttat ggcaatagga tttagtggtg gtaaactggg tgactttatc attcatcagt
    15301 tacaagaaca gggcatttct aaccaatttt tcaaaatcaa aggggaaacg agaaattgta
    15361 ttgctgtttt acatgaaggc atgcagacaa aaattttgga agctggtccc tacagtgatg
    15421 tggacgaagc cgaagtctct ttgagccaca tgagtactat tgctaagccg tttgatgtct
    15481 taatttctca ggcagtctgc ctaagagact agctactcac tattatcaag atttgatgac
    15541 tatggcaact tttaaacaat tatatcagca acttcaagta agagaggtat cacatgacaa
    15601 tcacagcaaa taaaagacat tacttagaaa aagtgagcca tcaaggaatc atatcagctt
    15661 tagcttttga tcaacgaggt gcactgaagc aaatgatggc agcacatcaa gaaggagaag
    15721 cgacagttac acagatagaa acattgaaag ttctcgtttc tgaggaattg acgccttatg
    15781 cttcatctat tttattagat ccagaatatg ggttgctggc tactaaggta agagctaatc
    15841 agacaggctt actgttagct tacgaaaaaa caggttatga tgctacaacc actagccgtt
    15901 tacccgattg cttagtggaa tggtcggtca aacggttgaa agcagcaggt gcagatgcta
    15961 ttaaattttt gctctattat gatgttgatg gagatgaaca aatcaattta caaaaacaag
    16021 cttacattga acgcattggt tcagaatgta cagctgaaga catccctttt tttcttgaac
    16081 ttttgagtta tgatgagaga atatcagata acaacagtgc ggcatacgca aagcttaagc
    16141 cacataaggt taacggtgct atgtcagtat tttcggataa gcgttttggc gttgatgtgc
    16201 taaaagtaga ggttcctgtt aatatggctt atgtagaagg ttttactgaa ggggaagtcc
    16261 attacagtca agctgaggct attaaagctt ttcaagacca agaagctgcc agccacctcc
    16321 cttatatcta tttgagtgca ggcgtatctg ccaaactttt tcaagagaca ttatactttg
    16381 cagcagcagc aggtgctcaa tttagcggtg ttttgtgtgg gcgtgccacg tgggcaggat
    16441 ccgttccagt ttatattact aaaggagagg acgaagcaag aaagtggcta tgcactgaag
    16501 gcttccaaaa tattgacgag ttaaacaggg tactcgaaga gacagctagc ccttggacgg
    16561 aaaaaatcta aaacaacctt atcctatcaa gcagctctca ttgatgtgat aagataagta
    16621 tggggataat tgtgtgaatc ttttgtagca aggatgaaaa tgggttttat ccttgctttt
    16681 ttatccttac attttatctt tttctactta ttttttaaag gaaaacgtgg taaactaaaa
    16741 gaagactaaa agacaatagg gtagcttatg acaattgata tgtgggaaat tttaaatatc
    16801 atcgggacca ttgcctttgc tctttctggt gctattgttg ccatggaaga agaatttgat
    16861 attttaggga tttttatttt aggtttcgtg acagcctttg gaggcggagc cattcgaaac
    16921 accctaattg gtttacctat agaggcctta tgggggcaaa agccagaatt cacttgcgct
    16981 ttctttgcta tggttttgat tatgcttttt cctaagctaa tagctagagg ttgggtacgg
    17041 gcagctgttt tgactgatgc cattggtctg gcagctttta gtgtccaagg agccttgcat
    17101 gctgtccgtc ttaaccagcc tttaagtgct gtcattgtag cagctgtgtt gactggtgca
    17161 ggcggtggtg ttgtcagaga tattctggca gggcgaaaac ctagtgtttt gcgtagtgaa
    17221 atctatgctg ggtggtctat tcttgcagct attgttttgc attttaaact agcagattca
    17281 acgattgaat gttatgcctt ggttgtttta ctcacgactt tacgcatgat tggcaaccgt
    17341 aaaaaatgga acctccctaa aatcaaatgg aaagaggaaa gataggatga ttcaactaat
    17401 tgcaattgat ttggacggca ccttattaaa ccaggacaaa cagattccaa aagagaatat
    17461 cacagccatc caagaagcag ctcagtcagg cttaaaaatt gtcttatgta caggccggcc
    17521 tcagtcaggg acaagaccct attttgacca attaggtttg acacaagagg aattcctcat
    17581 tatcaataat ggctgtagta cttatagtag tcctgactgg caattacgcc attcaaaaat
    17641 gttgaaggtt tcagatattg aattgctgga agagctaagc caaagttttc cagatattta
    17701 cctgacttta actgaggaaa acgattattt ggtgcttgaa gaagaagtgc cagaccttgt
    17761 gcaggaagat ggagatttgg tatttaccat tgtcaagcct gtctccttag cagagctgtc
    17821 ggacactcct cgcctgattt ttcaagccat gtacttagga gaaaaagctg cgctagatgc
    17881 ctttgaacgt gcggtgagaa atcagcttag tcagagtttc catgttgttc gtagccaaga
    17941 taatatttta gaaattctcc ctcagggagt cagcaaggca tcggctctca aagaattagt
    18001 agaagatttg ggtttaactg ctgaccaagt gatggctatt ggggatgctc ccaatgacat
    18061 tgagatgtta acttatgctg gtttaggagt tgctatggaa aatgcttcag cagctatcaa
    18121 gccattagct gacaaagtca ctttgactaa tgatatggcg ggtgtggccc aggctatccg
    18181 tcagtttgca cttgcagctc aaaaagacta actattaacg taaaaaggtt cccaaaagga
    18241 acctttttac gttaatagag gcaagcccat agcagccatt tcttcggcag gaacgatcat
    18301 gtcatctgaa atccagtttg tcaagacaga aacaatagca ttgctccaaa aaaggcgctt
    18361 atagtgcgat tgttttttgt cttttaattt ttgataagct ttcagacgtt tagtgactaa
    18421 tcgtgttaga agatggtgca ggtgatgttt gaaaatcatt ttaagaagct gagcttcttt
    18481 tttagcttca gtaaaaagat agagccaggc ttgataagcc tgagttttaa tatcaaattg
    18541 ttttagtcca cgaaaaatac gacggataac acctactaac agttgttcta taatggcttc
    18601 tttagaggta taattacgat aaaaagcgtt acgagatact cctgctttcg tcaccaattc
    18661 tgaaatcgta atatcaccta aaggttttat ttctagaaga tttagtaagg cgatttccat
    18721 agattcacgt gctatgcgtc gtgtttcttt attaaattgt gttaagtttt gaagtgatct
    18781 ttttgagagt gctttatcag tcatcacaaa tacctttcaa gtgttacatc tgatgagtta
    18841 tcagtagcaa aaaccgactt aataatgata taattttatg ggaaaagtct tagtttgtca
    18901 actaaagtta gttatcagga gcctcattac ctccttgtat agctctattg gatgacttta
    18961 ctaactattg aaaaggctag taataaaaca taggatttaa tgactagtta ttaccattag
    19021 cctagacatc ttagcgaagg cttaataatc tggtctttgt taccgaacac aaattagaga
    19081 aaaagaagga taaaaaatga cttggaaaat tgtaacagat tcaggatgtg atcttagaag
    19141 tttgactaga caatccaaag agttgcgctt tgaacgtgtg cctttgaccc tacagattgg
    19201 cacagaaatt tttagagatg atgatggatt agatatcgat aatatgatga caactatgta
    19261 ccagtcgtct aaagcaacga catccagctg tccaagccca gaagcctttt tgcaggccta
    19321 tagaggtgca gataatgtta tcgtgatgac aattacaggg accttatctg gcagtcataa
    19381 tagtgcacga ctggctaaaa atgaacttct tgaagaaaat cctaatgtca atattcacct
    19441 aatcgattct ttatcagcag gtggcgagat ggatttgctc gtactggagc ttgagcggtt
    19501 gatcaatctt ggattatcct ttgaagaagt tgtcaaacag atcacagctt atcagcaaaa
    19561 aacacgtttg atttttgtgc ttgccaaggt tgataatttg gttaaaaatg gtcgactgag
    19621 taaattagta ggtaaagtaa ttggcttact caatattaga atggttggga aagctagcaa
    19681 taaaggaacg cttgaacttc ttcaaaaagc gcgtggtcaa aaaaaagcag tttctgcttt
    19741 gatagaagaa attcaaaaag agggttatgt aggcggaaaa gtttacattg ctcatgctca
    19801 aaatccaaaa atttgtgagc agatcagtga aaaaattaaa tctttatatc ctgatgctgt
    19861 gattcaaact ggaaggacct caggtttgtg tagcttttat gctgaagacg gaggactttt
    19921 aatgggctac gaaatttaat gctatctaac aaaacaaaaa gctagactct tttgatttga
    19981 gctagctttt ttaagatatc aggaagacta atcactaaga tagccgtcta aaacgtttat
    20041 tgattgattt tacgaaaaat ttttgtatta ctcttttgat gattgcttgt ctcctgagtc
    20101 ggaaggttct gaagaggtct gaggtggaat tggatcagga tgactaggtt gtggcgtaac
    20161 atctgaagct gaagattctg agcttgggtc taaagtaggt gactgggaat tatcataagt
    20221 atctgagacg tctcctggga aaggtgtcca gtaagagtta tctgttattt ctccactttg
    20281 tcttggaaat tcatcaataa taggtttcgc tggtaaaata gcgtctaacc gagattgtaa
    20341 agcggctttt ttacctttgt tacttaagtc gttaactgct ttttgggcga tatctttaag
    20401 attttgattg agagtctcct cagcagcttt aactgcttga gtagctgcat cttcttgctc
    20461 ttgctttgct ttggcaacgt ttagctgttc ttgtagcgtt tttttcttag aagattctgc
    20521 taatttatca acagctttct gggcttcttg gatagcagct gcagtggcta aacgatcagc
    20581 gtcattaact gctttaactg ctttttttag taaactagca gcacttttag aatgtttagc
    20641 taccttatct tgcgtttgag ggtaggatcc aaaaataagg caggagccac ctattagagt
    20701 gatagttaga acactcaaca tccaataaag cgcctctttt tgtgatttag tcatggaatc
    20761 tcctttatga tattgtttca tcgtagccat attatacctc taattaaacg aaatgaaaag
    20821 atttgtgcaa aaacaaatga tgtaaaatat cggaaaagcc taacacctta tcaaaccaat
    20881 aatatgcaaa tgttgacatc ggtatacaca gaaacgctta aaaaaacctt gtgataaaga
    20941 ccgtaataca ttaaaataga atagactaaa aaattgagcg aggacaatat tgtgtcagta
    21001 aattggcaag acattgcatt tcatttcttt ggaggcttag gtctctttct tttcagtatc
    21061 aagtatatgg gagatggtct gcaacaagca gcaggagata aactaaggta ttacattgat
    21121 aaatacacca gtaatccctt ttttggtatc ttagtcggaa ttgccatgtc tgccttaatt
    21181 cagtccagtt caggcgtaac tgtgattacg gttggtttgg tttctgcggg tttgctaaat
    21241 ttgcgacaag ccattggaat tgttatgggg gctaatattg gaacgactat cacttcgttt
    21301 ttaattggtt ttaaactagg agattacgcc ttgcccatga tttttattgg tgcagcctgt
    21361 cttttcttta cctctaataa aaagctcaac aattttgggc gtatcatttt tggtgtcgga
    21421 ggaattttct tctcgctgaa cctaatggga gatgccatgg atcctttgaa atctgtgtca
    21481 gcctttcaaa attatttagc gacattaggt gataaaccct tccaaggtgt ctttatcgga
    21541 acggctttga ccatgttgat tcagtcatcg gcagccatta tcgggatttt acagggtctg
    21601 ttttcaggcg gtcttttgac cttacagggg gctattccta ttttgttagg ctcaaatata
    21661 ggaacctgca ttacagctgt cttagcagct ataggttcaa atattgctgc taaacgtgtg
    21721 gcagcagctc atgtgttatt taatttgatc ggtacaatta tttttatgat tatcctagtc
    21781 ccattcacat cattgatgtt atggctacaa tcaaagttga gtttaacacc tgagatgact
    21841 atcgcttttt ctcacggctc ttttaatatt actaatacca tcttattaat tcctttcatt
    21901 agtctactag ctatgattgt gactagactc attccaggag aagatgaagt ggtcaaatac
    21961 gaagcccttt atttagatcg cttacttatt acccaagccc cttctatcgc actaggaaat
    22021 gcccacaagg aattggttca tttagcctct tatgcgattc aagcttttga agcttcttac
    22081 agttatatta tgacagctga cgataaattt ggggaaaagg tcaagcgtta tgaaagagca
    22141 gttgatacca tcgatgagga gttaaccact tatttggttg atatttctaa cgaagccctc
    22201 agcccaagtg aaaatgaagt cttggctggt attcttgact cttcacgtga tttggaaaga
    22261 attggagatc attctgagtc tttaggcatt ctaattgaag gcatcatatc taaacaaatt
    22321 ggtttttcaa tatctgctcg tcaagaattg acagagatgt atcaattgac gcattgttta
    22381 acactggatg ccattagagc tatcgttgat agtgatacag acttggcgca aactattgtg
    22441 actcgccata aagaaattga agaaaaagag cgacgattac gtaaaaccca cattaagcgc
    22501 ttgaattgtg gagaatgtac ggcacaagca ggtattaatt ttattgatat tatttcccac
    22561 tatacccgca tcacagatca tgccttgaat ttagcagaga aagtactttc acaccagctt
    22621 taaaaaaaga gaaaacgtga atgatcatcc acgttttctt ttttgaatcc aaaaagtggt
    22681 atataccatt tacaaagtaa agaataagtc gtataatagg ggaaaaaggg aggaaaaaag
    22741 atgacatgct atcttaaagc agattgtttt tattacccaa cagaggtcaa accggctggc
    22801 tacctaagtc ttcatgatgg tgtttttggg gagtggacag aaattgttcc ggcagatgcg
    22861 caaatcattg actacaccgg ttatcaaatt gcaccaggtc ttgtggatac gcatatccat
    22921 ggctatgcgg gagctgatgt catggataat tctgcccaag ggattcatca aatgagtgaa
    22981 ggactattag caacaggagt aacgtctttt ttaccgacga ccttaacttc tacctttgag
    23041 cagttagaaa aagtatcagg gactattgct tcagttgctg atcaggtaaa aggtgctaaa
    23101 atacaaggta tttactttga aggtccttat tttaccgaaa aatataaggg tgctcaaaac
    23161 ccaagttata tgaaaacccc acgcttggag gaatttgatg cttggcaaaa agccgctaaa
    23221 gggctcatca aaaaaattgc ccttgctcct gagcgtgatg gtgtcaaaga atttgtgtcc
    23281 gcagtgacaa aacaaggcgt aacagtggcc ttagggcatt caaacggtac ttatcaagag
    23341 gctaaagaag ctgttcaagc tggtgctagt gtttgggttc atgcttataa tggcatgcga
    23401 ggattgaccc accgtgaacc aggaatggtg ggagcggttt ataatcttcc aaatacttac
    23461 gcagagttga tttgtgatgg ccaccatgtc tcaccaattg cttgtgatat tttgatgcag
    23521 caaaaagggc atgatcatgt agccatgatt acggactgta tgcgagcagg tggttcacca
    23581 gatggtgatt acctcttggg ggaattctct gtggttgtgg caaatggcac tgctcgtctt
    23641 aaagagagtg gaaacttggc aggatctatt ttgaaattaa aagacggcct tagaaatgta
    23701 gtggcctggg gaatcgcaac tccggcagaa gccattcata tggcaaccta tgtgccagct
    23761 gtttctgtcg gtattgacga cgtatgtggt caaatcaaag ctgggcatgc ggctgacttt
    23821 attgttcttg ataaggattt aacacttgtt gcaacctatt taaatgggca aaaagctttt
    23881 gatgcctaag agatacaaaa ggattggggt agctatgtct ggcaggccaa ttctttttgt
    23941 attttctttc tttatgatag aatagattac agtaatcgct tacagagatt gataaaagga
    24001 ggtttttgtg atggtaacaa cagtaaaaat gacaagtggc tatgagattc ccgttttagg
    24061 atttggaact taccaagcag ccgatggtga ggaagcttac caatctacct tagcagccat
    24121 caaggctggc tatcgtcata ttgacactgc agctatttat aaaaatgaag aaagtgtggg
    24181 tcgtgccatc aaggattctg gggttctacg agaagactta tttattacaa ctaaactctg
    24241 gaatgacgcc catagttatg aaggggcaaa agacgcttta gctgcttctc ttgaccgtct
    24301 tggtctggat tatgtggatc tctatcttat ccattggcca aacccaaaag ctctgcgtaa
    24361 cacttggaaa gaggcaaatg ctcaagcatg gcaatatatg gaagaggcgg ttgaagcagg
    24421 gctcattaaa agcattggcg tcagcaattt tatggtgcat catctagaag ctcttcaaga
    24481 gacagctaaa ataactccag caattaatca gatccgatta gccccaggtt gctatcaaaa
    24541 ggaagtggtt gattattgta aggcaaatga gattttattg gaagcttgga gtcctctagg
    24601 acaaggggag atttttgata atgagactat gcagcagtta gctaataaat atgacaaaac
    24661 agtggcacaa gctgccttag cttggtcgct ggcagaaggt tttattccct tgccaaaatc
    24721 ggtgcatgat gagcgcatca aggaaaatat ggctattttt gatgtgagct tgacaccaga
    24781 agataaaaaa acaattcgct atctctcagg aatgtcagcg attcctaacc ctgatactac
    24841 aagtttttaa gaaactacaa agatttagcc ataattcgct aaatcttttt tgaaaacggt
    24901 ttgacaaagg tttatcattt agctataatc tttattagtg ttttgtgaaa gaaactatta
    24961 ttgaaaataa ggagccgtat tgtgaccatc tttttagtga tgttagccat gtttactatt
    25021 cgtttagtat ttttgaagaa atcgataaca aatgaaaaag ccatattagc ccaaggagga
    25081 caggaatttg gtgctcagaa cacgaaattt ttgacgttac ttcacattat gatttatgtg
    25141 tttgcggtga ttgaagcgct gcttaaacaa attaagtttg atggcatcag ttttttaggt
    25201 cttttgttga tgttattatc agtagctgtt ttatatgaag tgacacgtat cttaggtgac
    25261 atttggactg taaaattgat gttagcaaaa gaccacaaat atgtggatca ctggttgttc
    25321 aaaacgatta aacatcctaa ctacttttta aatatcgccc ctgagctggt gggcatagcc
    25381 ttactttgcc atgctaaaat tacggctatg cttcttttcc catgctacat tgtagtaata
    25441 tatttacgta ttcgagaaga aaataagcta ttagcggaag tgattattcc aaacggtact
    25501 cgaacaaaac cttaagctca tgatggacaa actaatggct ttatgctaaa ctagactcta
    25561 ggaaaaagga tgcaagtatc ttatctaaag cgagttcggg gtagttagag ccgaatggta
    25621 ggactgcaga ttggcgcttc cgtttgggca gtgtgattaa gtatatttgt caatattgcc
    25681 caaaaagata ctatataaat gaagtaataa attagggtgg aaccgcgttt tgacgcccct
    25741 aggtcttatg atttaggagc atcaaaatgc ggttttttgg tgcctagacc tgtaatacat
    25801 gctagtaaca tcgtcaggta ttttactgat gacaaaaaaa cgattgtcaa tgatatacca
    25861 ttttactact atcaaaggag tacgaaatgt ctaaaaaact tacctttcaa gaaatcatct
    25921 tgaccttaca acaatattgg aatgatcagg gttgtatgct catgcaagcc tatgacaatg
    25981 aaaaaggagc tgggaccatg agtccttaca cgttcttacg tgctataggt cctgagccat
    26041 ggaatgcggc ttatgtagag ccatcacgtc gtcctgcaga tggccgttac ggagaaaacc
    26101 caaatcgtct ttaccaacac caccaatttc aagtggtcat gaaaccatca ccatcaaata
    26161 ttcaagaact ttaccttgct tctttggaaa aattgggcat caatccttta gaacatgata
    26221 ttcgttttgt tgaagataac tgggaaaacc catcaacagg atcagcaggg cttggttggg
    26281 aagtttggct tgatggaatg gaaatcacac aatttacgta tttccaacaa gttggtggcc
    26341 ttgcgacatc tccagtgaca gcagaagtaa cttatggtct tgagcgctta gcttcctata
    26401 ttcaagaagt ggattcggtc tatgatattg agtgggcacc aggtgttaaa tatggtgaaa
    26461 tcttcttaca acctgagtat gagcattcaa aatattcctt cgaaatatca gaccaagaca
    26521 tgctgcttga aaactttgaa aagtttgaaa aagaagcctc gcgtgcccta gaagaaggtt
    26581 tggttcaccc agcttatgat tacgtgctca aatgttctca tacgtttaac ctgctggatg
    26641 cccgtggcgc tgtttccgtc acagaacgcg caggctacat tgctcgtatc cgtaatttgg
    26701 cacgtgtcgt tgctaagacc tttgttgcag aacgcaagaa gcttggattg ccactgttgg
    26761 atgaagcaac acgcgcaatc ttgctagctg aagatgacga ataagtcgat ttaaagatga
    26821 tctagttgcg agctttgtaa tcttaagagc gagcaagaag tgggcgaccg aaggtcgccg
    26881 agtcaaggtt actcccgctg tagtgacaat tcggtaaggt tacttatcga acgagggagt
    26941 tggcgagact ttaggctggc taactaggaa tggaacactt ttgttttctg aaattatcca
    27001 ctggataatt tcactttgcg tcccccctac ctaagggaac aatcaaaaag atacttttta
    27061 gagaaaggaa ctgaacatgg gctacggact atgtcaaaaa gaccaagact tcctaggtgc
    27121 aagcacctgc gtcgctttgc tattttgatg gtgtccgctt aacgcccttg gtatcttaat
    27181 tatgagtaaa aatctactaa tcgaacttgg tctggaagaa cttccagcct acgtggtcac
    27241 accaagtgaa aaacaactgg gagagcgctt ggcgactttc ttaacagaaa atcgtctctc
    27301 ttttgaagat atacaaacct tttcaactcc acgccgtttg gcagtgcgtg tgtctggatt
    27361 agctgatcag caaacggatt tgactgagga ctttaaaggg cctgctaaga aaattgccct
    27421 tgatgctgat gggaatttct caaaagctgc tcaaggattt gttcgtggca aaggcttgac
    27481 cacagatgct atcgaattcc gcgaagtcaa aggagaagaa tacgtttatg tgaccaaaca
    27541 cgaagctgga aaacctgcta aggaagtgct tctaggcgtt actgaagtgt tgtcagcaat
    27601 gaccttccca gtcagcatgc actgggctaa caatagtttt gaatatattc gtcctgttca
    27661 caccttgaca gttctgttga atgatgaggc cttggaactt gacttcttgg atattcattc
    27721 tggacgcgtc agccgtggtc accgtttttt aggtactgaa accactatca caagtgctga
    27781 ttcttatgag gctgatttgc gcagccaatg cgtcattgtt gacgctaaag agcgtcaaga
    27841 gatgattgtg gagcaaatca agacgcttga agtggagcag ggtgtgcaag ttgacattga
    27901 cgaagacttg cttaatgaag ttttgaactt ggtcgagttt ccaacagctt tcatgggaaa
    27961 ttttgaggct aaataccttg atgtcccaga agaagtattg gtaacatcaa tgaaaaacca
    28021 ccaacgttat tttgtggtac gtgatcaggc tggtcacttg atgccaaact ttgtatcagt
    28081 tcgtaatggg aatgatcaag ccattgaaaa tgttattaaa gggaacgaaa aagttttggt
    28141 agctcgctta gaagatggtg aattcttctg gcgtgaggat cagaaattgc aaattgctga
    28201 tttagttgcc aaattgacca atgtgacctt ccatgaaaaa atcggctctc ttgctgaaca
    28261 catggatcgt acgcgtgtca ttgccgcatc acttgctaag gaagccaacc tatctgctga
    28321 ggaagtcaca gctgttgacc gtgctgccca aatttacaaa tttgaccttt taacaggtat
    28381 ggttggtgaa tttgatgagc tgcaagggat tatgggtgaa aaatatgctc ttctagctgg
    28441 tgaagatgct gctgtggcaa cagcaatccg tgaacactac ttacctgatg ctgcaggagg
    28501 tgcgctacct gaaaccaaag ttggcgttgt cctggccttg gcagataaat tggatactct
    28561 cttgtcattc ttttctgttg gattgattcc atctggctct aatgatcctt atgctctacg
    28621 tcgtgcaacg cagggtatcg ttcgtattct tgatcatttt ggatggcgca tcccaatgga
    28681 taagctggta gatagcttat atgacttgtc ctttgatagt ttgacctata ccaataaggc
    28741 ggatgtgatg aactttatcc gtgctcgtgt ggataagatg atgggcaaag ccgcaccaaa
    28801 agacattcgg gaagctattc tagcaagttc aacctttgtg gttccagaaa tgttagcggt
    28861 agctgaagct cttgtcaaag ccagccatac ggaaaattac aaaccagcag ttgaatcttt
    28921 gtcacgtgct tttaatttgg cagaaaaagc ggacgcttct gttcaggtgg accctagcct
    28981 ctttgaaaat gagcaagaaa acactttgtt tgcagctatt caaggcttaa ctctagcagg
    29041 cagtgctgcc cagcagttag aacaggtctt tgcccttagc ccagtgatta atgatttctt
    29101 tgataataca atggttatgg cagaagatca agcccttaag aacaaccgtg tagctatttt
    29161 atcagattta gtaagcaaag ctaagaccat tgctgctttt aaccaactaa acaccaagta
    29221 aatagaaagg aaagagccgt tggggactta gctagaaaag tgagttcagt acccgagtgg
    29281 aaagaacaag gcctttaaac attgcttttc acagtgagag tcaagtcagt tttttaagtc
    29341 ttacactttt ttcccaggct cttagtggct taacatggat cctaaaaaaa ttgctcgtat
    29401 caatgagctg gctaaaaaga aaaaaacggt aggcttgact ggacctgaaa aagtggaaca
    29461 ggcaaaactc cgtgaagaat acattgaagg ctatcgtcgc tctgtcagac accatatcga
    29521 aggcatcaaa ttggtggacg aagaaggaaa cgacgtgacc cctgaaaaat taagacaggt
    29581 gcaacgtgaa aaaggcttgc acggccgttc attagatgac ccaaaatcat aagacaaaaa
    29641 gcagctctga actgcttttt gtttatcgtg atgactgtta gcttttattt taaatggtat
    29701 gatacttcaa agtgaccaca cgtttttata ttgtatagtc aaaatattgg gaaaaaccat
    29761 gtgtttgagt tataatagaa gaaatcaaag aaagaaggta gtacttatga caattaaaaa
    29821 attctctcta ctagccatag ccagcctctc tttactcagt cttgcagcct gtgatatgga
    29881 tgataaagac gatcatatgg ataatcaacc aaagacaagt caaactagca agaaagttaa
    29941 actttcagaa gacaaagcca aatcaattgc gttaaaagat gcttctgtta cggaagctga
    30001 tgctcaaatg ctatcagtta cacaagataa tgaagatggc aaagccgtgt atgaaatcga
    30061 gtttcaaaac aaagatcaag aatacagcta taccattgac gccaactcag gggatattgt
    30121 ggaaaaatct tctgaaccaa tcaatgacta atcaagaact gtaatgaata agacagcctt
    30181 aactcttgtg tccaattgtc tttgcctctt aggcgcggct gtttgatacc gtgttgcctt
    30241 tgaggcttag ttctctttat gacccaatga tgactctgga ttagcagagt gattgttggg
    30301 tcttttgatt ttctttgata agaaagatta cctcgttaga aatgaggaga cgtggttgtc
    30361 atcagtggat ttttcaagat catctagtgg gataagctac ctatcttatc agcttttttt
    30421 tagctttaaa aattcttgat aatatatttc tgataaaatt agctataata gaatcaaata
    30481 ggaaaacgct tacttaaagg agggtaatga atgtcgcaag aaaagtatat catggctatt
    30541 gatcagggaa caacaagttc acgagcgatt attttcaatc aaaaagggga aaaggttagc
    30601 agtagccaaa aagaattccc tcagattttc ccacacgctg gttgggtaga gcacaatgct
    30661 aatcagattt ggaattcggt tcagtctgtt attgcgggtg cttttattga gtccagcatc
    30721 aaaccaagcc aaattgaagc aatcggaatt accaaccaac gtgagaccac agtggtttgg
    30781 gacaaaaaaa cgggtgtacc tatttataat gctattgtgt ggcaatcacg acagacggca
    30841 ccaattgctg agcaactcaa acaagatggc catacaaaaa tgatccatga aaagacaggc
    30901 cttgttattg atgcctactt ttctgcaacc aagatccgtt ggattttaga tcatgttcct
    30961 ggtgctcaag aacgagctga aaaaggcgag ttactttttg gaaccattga tacttggctg
    31021 gtttggaaat tgacagatgg tgctgttcat gtgacggatt attccaatgc ggctcgtacc
    31081 atgctctata atattaaaga cttaacatgg gatgatgaga ttttagagct tttaaatatt
    31141 ccaaaagata tgcttccaga ggttaaatcc aattctgaaa tttatggcaa aacagctgcc
    31201 tttcattttt atgggggcga agtgcctatt tcaggtatgg cgggtgacca acaggcagct
    31261 ttgtttggcc aactagcttt tgagcctggt atggtgaaaa acacttatgg cacaggttct
    31321 ttcattatca tgaatactgg tgatgagatg cagttatcga gtaataatct tttgaccacc
    31381 attggttacg ggattaatgg gaaggttcac tatgccttgg aaggttccat ctttattgca
    31441 ggctctgcca ttcaatggtt gcgagatggt cttaaaatga ttgagacttc accagaatct
    31501 gaacagttcg ctctagcatc aactagtgac gatgaagttt atgttgtgcc tgcttttacc
    31561 ggtttaggag ctccttactg ggattctaat gcgcgtggat ctgtctttgg cttgacacgt
    31621 ggtaccagca aagaagactt tgtcaaagca accttacaat ctatcgccta tcaagtgcga
    31681 gatgttattg ataccatgca ggtggattca ggtattgaca ttcaacagct ccgtgtggat
    31741 ggcggtgcag ccatgaataa tatgctcatg caatttcagg ctgatatttt aggaattgat
    31801 attgcccgtg ctaaaaattt agaaacaaca gctctaggag cagctttctt agcagggctt
    31861 gctgtggggt actgggaaga catggatgct ttgaaagagt taaatgccac aggccagctc
    31921 tttaaggcta gcatgaatga atcgcgcaaa gaaaaattat acaagggttg gaagcgcgct
    31981 gttaaagcaa ctcaagtttt cacccaagaa gaagatgcag atgacgatgc aaaataacta
    32041 gaggaggatg gaatggaatt ttcaaaagag acaagacgct tagctctcca aaaaatgcaa
    32101 gaaagagatt tagacctact gattattggg ggaggtatta cgggtgctgg tgtggcgctt
    32161 caggcggcag ctagtggcct agatacgggt ctgattgaga tgcaagattt tgcgcaagga
    32221 acctctagcc gttcaaccaa attggttcac ggggggcttc gttacttgaa acaatttgat
    32281 gtggaggtag tttcagatac ggtgtcagag cgggctgtgg tgcaacaaat tgcaccccac
    32341 attccaaaac cagaccctat gttattacct gtttatgacg aacctggcag cacctttagc
    32401 atgttccgtt tgaaggtcgc tatggatttg tatgatcttt tagcaggcgt gtccaatacg
    32461 ccagcggcca acaaggtgtt aaccaaagaa gaagtcttaa aacgagaacc agacttaaaa
    32521 caagaaggct tgcttggtgg tggggtttac cttgatttcc gcaataatga cgcaaggctt
    32581 gttattgaaa atatcaaacg agctaatcgt gatggggctc ttgttgctag tcatgtgaaa
    32641 gcagaagatt tcttgctaga tgacaatggt aagattattg gtgtgaaggc gcgtgatctg
    32701 ctgtcagatc aagaaatcat tatcaaggct aaattagtca tcaacaccac aggtccatgg
    32761 agtgatgaga ttcgtcaatt ctctcataag ggacaaccga ttcatcaaat gcgccctaca
    32821 aaaggggtgc atctggtagt ggaccgtcaa aaattaccag tgtctcaacc tgtctatgtt
    32881 gacacagggt taaatgatgg tcgtatggtc tttgtcttgc cacgtgaaga aaaaacttat
    32941 tttggaacaa cggacacgga ctacactgga gacttggagc acccacaagt tactcaagaa
    33001 gatgtggatt atttgttagg cgttgtcaat aaccgctttc caaatgccaa cgtgaccatt
    33061 gatgatattg aaagcagttg ggctggtctt cgccccttgt tatcaggtaa tagtgcttct
    33121 gactacaatg gtggcaacag cggtaaagtc agtgatgata gttttaatca cttggttgat
    33181 actgtcaaag cctatattaa ccacgaagat agccgagaag ctgttgaaaa agctattaag
    33241 caggttgaaa ccagcacatc tgaaaaagaa ttggatccgt ctgcagtgtc acgaggttca
    33301 agttttgacc gtgatgagaa tggactcttt accttggcag gtggtaagat taccgactat
    33361 cgcaaaatgg ctgaaggagc attgacaggg attattcaaa tcctcaaaga agagtttggc
    33421 aaatccttca agcttatcaa ttcaaaaacc tatcctgttt caggaggtga aatcaatcca
    33481 gccaatgtag attcagaaat agaagcttat gctcaattag gaactcttag tggtctttcg
    33541 atggatgatg ctaggtattt ggcaaacctt tatggttcta atgcgccaaa agtctttgcc
    33601 ttaactcgtc aattaacagc agctgaaggg ttaagtttag ctgaaacctt gtccttacat
    33661 tatgcgatgg attatgaaat ggctcttaaa ccgacagatt atttcttgag aagaacaaat
    33721 cacctcttat ttatgcgaga tagcctagat gctttgattg acccagtgat taatgaaatg
    33781 gctaaacatt ttgaatggtc tgatcaggaa agagtggcac aagaagacga tcttcgtcgc
    33841 gtgattgcag acaatgattt gagtgcccta aaaggccagc aggagggtta atatggatat
    33901 ctttggtgaa tttttgggta cagccttatt ggttttacta ggaaatggtg tggtagcagg
    33961 ggttgtctta cccaaaacga aaacccatgc ttctggctgg atcgtgattg cgactggctg
    34021 gggaattgca gttgctgtgg cagtttttat cagtggcaaa gttgcccctg cccacctcaa
    34081 tccagctgtt agccttgctt ttgccatgag tggaaccatt gcctggtcaa cagccattgc
    34141 ctatagcctt gcccaactat tgggagctat ggttggttca actctggtat tccttcagtt
    34201 caggccacat tatctggctg ctgagagtca ggctgatatt ttagggacat ttgcgacagg
    34261 tcctgctatt cgagatacca gctcaaacct attgagtgaa atctttggga cctttgtctt
    34321 gatgcttggt attttggcat ttggcttata tgatatgcca gcaggactag gaaccctctg
    34381 tgtaggtacc ttggttattg ggattgggtt atccttagga ggaacgacag gttacgccat
    34441 taacccagcg cgtgatttag gacctcgtct agttcatgct attctaccac tcaacaacaa
    34501 gggagattct gattggtctt atgcttggat acctgttgta ggaccaatta ttggagcggc
    34561 cttagcagtt ttgcttttcc aagtcatgtc ctaaccatca ctaaaggggt tggctatcat
    34621 aggccagctc ctttgctttt tctttatagg attaggtaaa acgctttcat atcttaaaaa
    34681 taactgctat aataatacta ttatgacatt aacgacaaag accatacatg ttatcggggc
    34741 atcctttgct ggtctagcct ttgttgataa atataaggac ctcaatccag attcccaaat
    34801 cattctcatt gataaggaaa gctgtcccaa ttatattcca aatggtatca accaattatt
    34861 cagaggagac attcaagacc tgtctgatgc tatgtgggga agggcctgcc tagcagcaca
    34921 aatagaatct aaccaccggt ttattcaagc agaagtgtta gctatcgaag ctccctcaaa
    34981 caccttgctt ctcaaagaca gtcaaggacg agtttttgaa gaaggttatg aaacgcttgt
    35041 ttgtgccatg ggtgctagcc cccagtctca ttatattgag acgtcacaga ccaacaaagt
    35101 tttggtaacc aaatattatg aagaaagcca agctagttta aaactgattg aggctagcca
    35161 agaggtcttg gttattggtg ccggcttgat tggcttagac cttgcctaca gtttgtcctt
    35221 acaaggcaaa cgggtcaaac tgattgaagc agccgaacgt ccagatttct atcagactga
    35281 cgcagaactg atagcccctg taatggcaga aatgtccact caccatgtca cctttattaa
    35341 taataagcgt gttacggcta tccatgagat agagggaaaa gttgtcgctc ataccgagca
    35401 aggggacacc ttccaaggtg atttggctat tttagccatt aactttaggc caaatacaca
    35461 ccttttgcaa ggacaagtgg cttgtgctct ggataagacc attttggtca atgaaaactt
    35521 acagaccagc caggctaata tctatgccat tggagacatg gtttcattgc attttggtat
    35581 attgggaatg gattactata cacctttgat taaccaagca atgaagacag gtcaggctct
    35641 ggctctgcat ttagctggat atcctattcc tccattacag acggtcaagg tattaggaag
    35701 ttctcacttt ggttattatc gcgcgagtgt tggtgtgaca gaagaagaag cagagcttta
    35761 tatggatacg tgtagctatt tgtaccaaaa tggggattct aaaaacctct tttggctgaa
    35821 attaatagct agaaaaacgg atgggatttt aataggcgct cagctcctat ccaaaaccaa
    35881 tgctcttgtc atcgccaatc aattaggtca ggctctggct ctgaaagtta cagacggtga
    35941 cttagcattt caagattttc tctttttaca aggtcacagt gatttggctt atcatcttca
    36001 tgaagcttgt ctcaaattat ttgaaaagag gctccgccat gaagattgaa gacttaatgg
    36061 ataaagaacg aaaagctcaa tatcgtctct tggtgaccct ttatcatgct aaagaaacgt
    36121 taaggctaaa agacctaatg aggctatcaa acttgtctaa agtgacccta ttaaaataca
    36181 ttgacaacct aaatcatttg tgtcgagaac aaggtttagc ctgtcaatta cttttagaaa
    36241 aagacagtct gagcttaaaa gaaaatggcc agttccattg ggaggatttg gtagctctgc
    36301 ttctcaaaga atcggttgcc tatcagatat tgacctacat gtactgccat gaacacttta
    36361 acatcaccaa cttatccgtt gaactgatgg ttagtgaagc tactctaaac agacaactag
    36421 ctcacctcaa ccagctttta tcagaatttg atctggcgct ctcgcaaggc agacaactgg
    36481 gaagtgagct tcagtggcgt tatttctatt tggaattgtt ccgtcatacc ttgaccagac
    36541 aaggcataga cgccttggtc aatcagttag acgcttcgca tttggctacc ttaattgagc
    36601 ggctgattgg ccagtcatta tcggcagaag ccttagaaca attattgatt tggttggcga
    36661 tttcgcaagc gcgaatgtcc tttcaaaaag gctacaatga tcatttttta agagatagtg
    36721 actttatgac atctaatatt ttctttaagc gcttagaaag catgctttta cattacttaa
    36781 gacgctatgc ccttgaattt gatgcttttg aagctaagag cctctttgtc tttttgcatg
    36841 cttatcctct acttcctatt gccagcatga agtatagttt gggttttgga ggtcctattg
    36901 ctgatcacat ttcagaagcc ttatggcttt taaagaaggc tcatgtgatt atccatcaga
    36961 caaaggagga aatcatttat ggcttgggga tatttttttc caaagcttac ttttttaaag
    37021 gagctatcct tagccaacca accaatagcc aataccttta tcaattagta ggcgaagata
    37081 aaagagcgct tttaagggtt atcataaacc atttagtgct gcaaatgggt caggaaacgg
    37141 atttcagtca gcagttgagt gacgacattt tagcattgtt ggttttttcc attgagcgcc
    37201 accatgagcc tcttttagta gggcttgcct tggggcaaaa taaggtggag gcagccattg
    37261 cggaattggc cattagacgt catttggggc atcgtaggga ttttcaatta atgccctatg
    37321 atcatcaaaa agtttatgac tgtctgatta cctatcagac tgtctattta ccgagacaag
    37381 atttacccta ttaccggtta aaacagtata gctcccccta tgaactcact gccttagaag
    37441 cttttctcaa agaccttttc cagcaaaaaa atacacgaga agaggaactc tcactcagcc
    37501 cagcagctaa gtctagtttt gcacacaaaa ctgtgtgata cttggtcttt tctaaagact
    37561 gttacacttt tataataata aagtaggata gtctaaaggt agatgatttg aaaaagctta
    37621 tcaaatagag cttaacaaaa tcgaaaattt ttggaggtaa gaaatgaaat ttttcctaga
    37681 tacagctaat gtagccgcta ttaaagcgat taatgaactt ggcgttgtag atggtgtcac
    37741 caccaatcca accattatct cacgtgaagg acgtgatttt gaaacagtta tcaaagaaat
    37801 ctgtgacatc gtcgatggcc caatcagtgc tgaagtgaca gggttaacag cagatgctat
    37861 ggtagaagaa gctagaagca ttgctaagtg gcatgacaat gtcgtcgtta aaattccaat
    37921 gaccacagaa gggttaaaag caacaaacat tctttcaaaa gaagggatta aaactaatgt
    37981 gaccttgatt ttcacagtaa gccaaggttt aatggcaatg aaagcaggcg caacttacat
    38041 tagcccattt atcggtcgtt tggaagatat tggcacagat gcttatcaat taatcagcga
    38101 ccttcgtgaa atcattgact tgtacgattt ccaagcagaa atcatcgcag caagtattcg
    38161 tacaacagcc catgtggaag ctgttgctaa attaggcgca cacattgcga cgattccaga
    38221 cccattattt gctaagatga cgcaacatcc cttaacgaca aatggattga aaacatttat
    38281 ggaagattgg gcttccttta aaaaataagg ctaaagagtc tgtggccact gtgtcaacag
    38341 gctctcttat attcatcgtc aaaaagaatt caccaatgat gtccaaacta gtttttttct
    38401 ggcaaaatag agaaaaagaa tttagggact ttggaggatt ttctgaaaaa agcgtctatt
    38461 tttgtgatac aatagacaat agaaaacgtt taattttagt tgtaataaac agagaggttt
    38521 tacttatgac atttgatgca attgaccagt tggcagtaaa cactgtccgc accctatcaa
    38581 tggatgccat tcaagcggca aattctggac acccaggact tccaatgggg gcagcaccta
    38641 tggcctatgt tctttggaat cacttcatga acatcaatcc caaaacaagc cgtaattggt
    38701 caaacagaga ccgttttatc ctatcagcag gtcatggaag tgccatgctt tatagcttgt
    38761 tgcatttagc aggttatgat ttatctgtag aagatttaaa gaacttccgt caatgggctt
    38821 ctaaaacacc aggtcaccca gaagtgaacc acacagacgg tgtcgaagca accacaggac
    38881 ctcttggtca agggatcgca aatgccgttg gtatggccat ggcagaagct cacctagcag
    38941 ctaaatttaa caaaccaggc tttgacatcg ttgatcacta cacatttgct ttgaatggtg
    39001 acggtgacct tatggaaggg gtcagccaag aagcagcaag tatggcagga catttaaaac
    39061 ttgggaaatt ggtcttgcta tatgattcaa acgacatctc tcttgatggt ccaacctcta
    39121 tggctttcac agaagatgtg aaaggacgtt tcgaagctta cggttggcaa catatccttg
    39181 tgaaagatgg aaatgattta gaagaaattg cagctgctat tgaagcagct aaagctgaaa
    39241 ctgagaaacc aaccatcatc gaagtcaaaa ccattattgg ttttggtgcc gaaaaacaag
    39301 gaacttcagc tgttcacggc gctcctcttg gagcagaagg cattgctttt gccaaaaaag
    39361 cttaccaatg gactcatcaa gattttgaag tgcctgctga agtaacagag cgctttgctc
    39421 aaggccttca agcgcgtggt gaaaaagcag aacaagcctg gaatgacttg tttgcagttt
    39481 atgaagcaga atatccagaa ttagcagcag aataccaaaa agcctttact aacgaagctg
    39541 ctcaagtaga acttgaagcg cacgaacttg gtagttcaat ggcgagtcgt gtatccagtc
    39601 aacaagccat ccaacaaatc tcagaacaag tagcctcttt ctggggtgga tcagcagatc
    39661 tttcagcatc taacaacaca atggttaagg ctgaaacaga tttccaacca ggtcactacg
    39721 aaggccgtaa catctggttt ggcgttcgtg aatttgccat ggctgcagcc atgaacggta
    39781 ttgcccttca cggcgggaca cgcgtttacg gcggaacctt ttttgtcttc tcaaactatc
    39841 ttcttccagc tgttcgtatg gcagcgctcc aaaacttgcc aacagtttat gtcatgacac
    39901 atgattctat cgctgttggt gaagatgggc caacgcacga accaattgaa caattggcaa
    39961 gtgttcgctc aatgcctaac tttaacgtta ttcgtccagc agacggtaac gaaacaaatg
    40021 ctgcttggaa acgtgccatt gctgaaacag atcgcccaac catgcttgta ttgacgcgtc
    40081 aaaaccttcc agtgcttgag ggcacaaaag aattggcaga agatggtctt aacaaaggag
    40141 cttacatctt gtcagaagcc aaaggcgacc ttgaaggtat cttgattgca acaggttcag
    40201 aagtgaaact ggcaatggat actcaagaag ctcttgaagc agaaggcatt catgtgcgtg
    40261 tcgtatctat gccatcacaa aatatctttg acgaacagtc agcagagtat aaagaaagca
    40321 tcctaccagc agctgtgact aaacgtctcg ctatcgaagc aggttcaagc tttggctggg
    40381 caaaatatgt tggcctagca ggaaaaacat tgaccattga cacttggggt gcttcagctc
    40441 caggaaatcg tatcttcgaa gaatatggtt tcactgtggc aaatgcaact gagttataca
    40501 aatcactcta ggttacacat cccaaacaga gtaaatcaaa tgatttactc tgttttttta
    40561 aaataaaata accagttctt tcttttcctc aaactgccta atacacttct tttgataaga
    40621 agtcattgac aaaacgattc ataaaaatta taatttaatt gttattgtat tagcgtaaaa
    40681 gtacaaaaaa taaaggagta taaatggaaa agaaacgaca gcgcttatac gatgtgatta
    40741 gacaggctta tgattatcca gaaaaccgtg aaaatgtggc cttgtcgcag ctacttttag
    40801 cagcttctaa tagattaatt aaacacagta acccacttct tgttgcctac caactaaacc
    40861 aagatgtgga taattactta cttgataatg acattttact tccaaaatcg ctttgccgtt
    40921 ttaagcagtc attggaaaag tatattttag aggaagcctc atcagtctag ggagaaaaag
    40981 agaagaaaag gaaaaaggat accatgatag aatttaaaca tgtttcaaag ttgtatggcg
    41041 acaaagaagc tctcagcgat ttaaacgtca ccatcaatga cggtgaaatt tttggcctta
    41101 ttggacataa tggtgcaggt aaaaccacga ccatcagcat tttgacttct attatcgaag
    41161 cgagttatgg cgaagtattt gtggatggtc aacttctgac agaaaataga gaagctatca
    41221 aaaaacaaat tgcttatgtg ccagattctc ctgatatttt cttgaatcta actccaaatg
    41281 agtattggca gtttttagct aaaatttatg gggtgtcaga tgaggaccga gaagaacggt
    41341 tagcacagct gacaacactt tttgaactga aggaagaagt taatcaaacc attgatagtt
    41401 tctcacatgg gatgcgccaa aaagtgattg tgattggtgc tcttgtatca aatcctaaca
    41461 tctggatttt ggatgaacca ttaacaggtt tggatcctca ggcttctttt gaccttaagg
    41521 aaatgatgaa agctcatgca gcttctggac atacggtctt attttcgact cacgtgctat
    41581 cagtcgctga gcagttatgt gatcgtatcg ggattctaaa aaaagggaaa ttgatttttg
    41641 ttggaacgat tgatgagttg aaagaacacc atcctgacaa agacttggag agcatctacc
    41701 ttgaactcgc aggtcgtaag gcgcaagaag aggggtgatg ggatgaattg gtctactatt
    41761 tgggaactta tcaagataaa cattctttat tccaatccac aaagtttagc taacctcaaa
    41821 aagcgtcagg aaaaccaccc gaaagaaaac ttcaaagcct ataaaagcat gatgaggcaa
    41881 caggccttaa tgattgccat gtttttggtg atttaccttt ttatgtttat tggtgtcgat
    41941 ttcagtcatt atccaggact tttctccttt gacgttgcta tgttttttat catgtcaacc
    42001 ttgacagcct ttagctctct ttacaccatt ttttatgaaa gtaatgactt aaaactttat
    42061 attcacttac cagtaacgtc agaagaactc tatattgcaa aaattgtctc gtcgctaggg
    42121 atgggtgctg tctttttgat gcctcttatc tctcttctct taattgccta ttggcaacta
    42181 ttgggaaatc ctttgtctat tttagtagct attgtgctct ttttagtgtt actagtgagt
    42241 tcgatggtat tagctattta tatcaatgct tgggtgggca aaatcattgt aaggagtcgc
    42301 aagcggaaac tcatttcaac tatcatgatg tttgtctcaa cttttggagc tttcgtctta
    42361 atttttgcga ttaatattag taacaacaag cgtacgatga cggatggcgt atttactgat
    42421 tacccaacta ttccgtattt caaaggattc tatgatgtta ttcaggcacc gttttctact
    42481 gcggccctcc ttaacttttg gttgccattg ctactgattt tagctatggt atatggtatt
    42541 gtcacaaaag tgatgccaac ttattatcgt gaggcttttt atattagtaa cgagaacaaa
    42601 gccaagcaaa ctaaaaaacc agttaatcgt cctcatcaga atcaatcact gacgcagttg
    42661 ttgcgaaaac atcatctttt aacgttacaa aatgcaactt tactgacaca aacctatctc
    42721 atgcccctga tgtatgtgat gctttttatc ggtccaagtt tgtcacgtgg cacaggtttc
    42781 tttaagcata tttccccaga ttactttggg gtggccttat tatttggggt cagtttgggt
    42841 gtcatgtgtg caacaccaac cagctttatt ggagtaggta tttcacttga aaaggataac
    42901 tttaccttta ttaaaagctt accaataacg ttaaaaaaat tcttgctgga taaattttgc
    42961 ttgctcgtcg gtctgcaact gattgtgcct atggtgattt atcttgtgtt tggtctcttt
    43021 gtgttgcacc tgcatcctct tctaacgatt gctttttgtc ttggctacgc cctgtctctg
    43081 attgtgcaag gggaattgat gtatcgtcgt gactaccggc ttctggattt aaaatggcaa
    43141 gacatgacac aactctttac cagaggaaat gggcagtggc taacaatggg ccttatcttt
    43201 ggtaatttaa tagtcacagg tgtagtaggt tttggagctg ttatcattgc caatattatc
    43261 caacaacctc tgttgattag tattctattg agttgtctga cactaatggt tttagggatt
    43321 gctcacttat ggattcaaaa aaccttctgg aaaagcttag agaagcttta acgaagagag
    43381 atcattattc ggaaaaacag tcctctgggg ctgtttttat gctataattt gggtatgatg
    43441 aaacgacaat ttgaagatgt aacacgtatc gtaattaaaa ttggcactag ttcattagtg
    43501 ctaccaacag gtaaaatcaa tttggaaaaa atcgaccaac tggcttttgt gatttcaagt
    43561 ttgatgaata agggcaagga agttatttta gtatcatcgg gagcgatggg atttggacta
    43621 gatattttga aaatggaaaa acgtccgacc aatcttgcta aacaacaggc agtttccagt
    43681 gttggtcagg tggccatgat gagtctttat tcacaaattt ttgcacacta ccaaaccaac
    43741 gtgtcacaaa ttttattgac gagagatgtg gtggttttcc cagaaagtct agccaatgtt
    43801 accaatgctt ttgaaagtct gattagtttg ggcattgtgc cgattgttaa tgaaaacgat
    43861 gcggttagtg tggatgaaat ggatcatgcg actaaatttg gagataatga ccgtttatct
    43921 gctgtcgttg ctggtattac aaaagctgat ttattaatca tgttgtctga tattgatggc
    43981 ctttttgata aaaaccctac tatttatgag gatgcgcagt tacgcagtca tgttgcagtc
    44041 attacgcaag aaatcattgc gtcagcaggt ggtgcgggca gtaaatttgg cacaggtggg
    44101 atgcttagca aaattcagtc tgctcagatg gtatttgaaa acaaaggaca gatggtcttg
    44161 atgaatggag ctaatcccag agatatttta agagtgcttg aagggcaacc actaggcacc
    44221 tggttcaagc agatagaaga ggtaacacat gactgatatg agaaggttag ggcaaagggc
    44281 aaaacaagct agtcttttaa tagcaccttt gtcgacgcaa attaaaaatc gctttttaag
    44341 caccttagca aaggcattag ttgacgatac acagactctc ttagccgcta accaaaaaga
    44401 tttagcgaat gctaaagaac atggtatttc tgacatcatg atggatcgct tgcgcttaac
    44461 ttcagaacgg attaaagcga tagcacaggg agttcaacag gttgctgact tagctgatcc
    44521 aatcggacaa gtcattaagg gctatactaa cctcgatggc cttaaaatcc tccaaaagag
    44581 agttcctcta ggggtgattg cgatgatttt tgagagtcgc ccgaatgttt cagtggatgc
    44641 ttttagtctg gcgttcaaaa ccaataatgc catcatccta aggggaggaa aagatgccct
    44701 tcactcaaat aaagctttgg tgaagttgat ccgacaaagt ctggaaaaat cagggatcac
    44761 cccagatgct gtccagctag ttgaggatcc ctcacatgct gttgctgaag aattgatgca
    44821 ggcaacggac tatgtggatg tgttgattcc acgtggtggt gctaaattaa ttcagaccgt
    44881 taaggaaaaa gcaaaagtgc ctgtcatcga aacaggtgtg gggaatgtgc atatctatgt
    44941 ggatgctcaa gctgacttag atatggctac taatattgtt atcaacgcta aaacaaagcg
    45001 cccaagtgtt tgcaatgcag ctgagggatt ggtaattcac gaagctgtag cagcaaggtt
    45061 tattcctatg cttgaaaaag cgatcaatca ggttcagcca gtagaatggc gggcagatga
    45121 caaggctttg ccgttatttg agcaggcagt gcctgcaaaa gcagaggatt ttgagaccga
    45181 atttttagat tatatcatgt ctgttaaggt tgtttcctct ttggaagaag ctatttcttg
    45241 gataaatcag catactagtc accattccga agctatcatc acaagggata ttaaggcagc
    45301 agagaccttt caagatttag tggatgctgc tgctgtttat gtcaatgctt caacacgatt
    45361 taccgatggt tttgtttttg ggcttggtgc tgaaattggg atttctactc aaaaaatgca
    45421 cgcccgtggg ccaatggact tagaagcctt gaccagcact aaattttata ttaatgggga
    45481 tggacatatc agagaataag ctactagaag gtcccttaat gactaatgaa agagaaaaag
    45541 aagaagcaaa ggttgcttta gaagaacttt tacatcagat tcaggtgatt catttggaat
    45601 tgaaaattgt ccgaaattgg ggctattttg atctttttgg agacggtatg cttgtcagct
    45661 ggatcaaacg tcgaaaaatg agcaaggtca accaattatt aatagactta caagttactc
    45721 ttcaaaactg ccgaagatag ctggcagacg tcgataggct attattagct gacttaccga
    45781 atatgagttg ggataaaatc tttgatattt ggttagacaa cctgctgaca gattgttgtg
    45841 tgattaaaga agctaaaaat tttttacaag tagttgaaat tctagaggca gcggctaagt
    45901 cagtgcttgc taagttggct gatagcgaac aaaaagaaca agattagaga agaggaagat
    45961 tcccacgaga atgaaatcct tttctcttac tttttcattt ctaaaccttt tgaagtatgg
    46021 tacaattaaa gttatgacaa aagaatttca tcacgtgacc gtactccttc acgaaacagt
    46081 ggacatgctt gacataaagc ctgatgggat ttatgttgat gcgacgctag gtggctcagg
    46141 ccactcagct tatttgttgt ccaaacttgg tgaagaaggg cacctctatt gttttgacca
    46201 agaccaaaag gctattgaca atgcacaagt taccctcaaa tcttatattg acaaaggaca
    46261 ggtaactttt attaaagata attttagaca cctcaaagca cgtttaacag cgcttggagt
    46321 tgatgaaatt gatggtatct tatacgacct tggtgtttcc agcccgcaat tggatgaaag
    46381 agaacgaggg ttttcttata aacaagatgc tccattggat atgcgcatgg atcgtcagtc
    46441 gctcttaaca gcttacgaag tggtgaatac ctatccattc aatgatttgg ttaagatttt
    46501 cttcaaatat ggtgaagata aattctccaa gcagattgct cgaaaaattg aacaagcaag
    46561 agctattaag cctattgaga caacaacaga gttggcagaa ttgattaagg cagcaaagcc
    46621 agctaaagag ttgaagaaaa aaggccaccc tgctaaacag atttttcaag ctattcgcat
    46681 tgaagtcaat gatgaattgg gagcggccga tgaatctatt caggacgcta tggaattatt
    46741 agcccttgat ggtcgtatct cagtcattac cttccattct ctggaagatc gcctaaccaa
    46801 gcagttgttt aaagaagcta gtacggtgga tgtgccaaaa gggcttcctc taattcctga
    46861 agatatgaaa cctaagtttg aacttgtttc acgtaagccg atcttaccta gtcattcaga
    46921 gttaacagct aataaaaggg cacactcagc caagctacgt gttgccaaaa aaattcggaa
    46981 ataagcctat gacaaatgaa aaaagaactc aggcagttac aaatgccttg caaaaacgaa
    47041 taaaaacttt ctctagaatt gagaaggctt tttatactgc tatcattgta acagcgatta
    47101 cgatggcagt aagtattatt tacctgcaaa gtcgtaagtt gcagctacaa caagaaatca
    47161 ctagtttaaa tagtcatatt tcggatcaaa aactagagct taataatgcc aagcaagaag
    47221 taaatgagtt atctcgccgt gatcgtatta tagatattgc tggtaaagca ggcttaagca
    47281 accgtaataa taatatcaag aaagtcgagt aatctatgaa aaaatggcaa aaatatgttt
    47341 tagattatgt tgtgcgcgat aggagaactc cagtcgaaaa tcgcgttcga gttggacaaa
    47401 atatgatgct cttaactatc tttattttct ttattttcat tattaatttc atgattatta
    47461 ttggaacaga tcaaaagttt ggagttagtt tgtcagaagg ggcgaagaag gtttatcaag
    47521 aaaccgttac gatccaagct aagcgtggga ccatttatga tcgaaatggt acagctattg
    47581 cagtggattc tacgacttat agcatatacg caattttgga taaatcattt gtctcggctt
    47641 cagatgaaaa gttatatgta caaccttctc agtatgaaac agtagctgat attttaaaaa
    47701 agcatctggg aatgaaaaaa acagatgtaa ttaaacagct taagcgtaaa ggacttttcc
    47761 aagtctcgtt tggaccgtca gggtctggca tttcatatag tactatgtct actattcaaa
    47821 aggctatgga agatgccaaa atcaagggaa ttgctttcac aactagtcct ggtcgtatgt
    47881 atccaaacgg gacatttgct tcagaattta taggcttggc atctctaaca gaagataaaa
    47941 agacaggtgt taagagttta gttggaaaaa caggtctaga agcttctttt gataaaattt
    48001 tatcaggtca agatggtgtt attacttatc aaaaagatcg aaatgggacc acactcttgg
    48061 gtacaggtaa gactgtcaag aaagctattg atggcaaaga tatttacaca acgctatctg
    48121 agcctatcca gaccttctta gaaacccaga tggatgtttt tcaagccaaa tcaaatggtc
    48181 agttggccag tgcaacactt gttaatgcta aaactggtga aattttggca acaacacaac
    48241 gccccactta taatgctgat actctgaaag gacttgaaaa tacgaactac aaatggtaca
    48301 gtgcacttca tcaaggaaat tttgagccag gctcaaccat gaaagtgatg actctggcag
    48361 cggctattga tgataaagtt ttcaacccaa acgaaacctt tagcaatgct aatggtttaa
    48421 caattgcaga tgctactatt caagactggt ctattaatga aggcatttcc acaggacagt
    48481 acatgaatta tgcacaagga tttgccttct caagtaacgt tgggatgact aaacttgaac
    48541 aaaaaatggg taatgcaaaa tggatgaatt atttgacgaa gttccgcttt ggttttccta
    48601 ctcgttttgg tttaaaagat gaagacgcag gtatatttcc ttctgataat atcgtgactc
    48661 aagctatgag cgcttttggt caagggattt ctgtaaccca gattcaaatg cttagagcct
    48721 ttactgctat ttctaataat ggtgagatgt tagagccaca atttatcagt caaatttatg
    48781 atcctaacac agcaagtttt agaacggcaa ataaagaaat tgttggaaaa cctgtatcaa
    48841 aaaaagccgc tagtgaaaca agacaataca tgattggtgt aggaacagac cctgagtttg
    48901 gaacactcta ttcaaaaaca tttggaccaa ttattaaagt gggtgattta cctgttgctg
    48961 ttaaatcagg aacagcacaa attggttcag aagatggaag tggttatcaa gatggtggat
    49021 tgactaacta tgtctattcg gttgtggcaa tggtgccagc tgataaacca gactttttga
    49081 tgtatgttac tatgactaaa ccacaacatt ttggtcccct tttttggcaa gatgtggtta
    49141 acccagtatt ggaagaagca tacttaatgc aagatacact aactaagcca gtagtatcag
    49201 atgctaatcg tcaaacaact tataaattac caaactttgt aggaaagaat cctggtgaga
    49261 catcaagcga gttgcgtcga aatcttgtcc agccagttgt ccttggtact ggcagcaaga
    49321 tcaaaaaagt atcgcatcag cccggtcaaa cgttaacaga aaaccaacaa gttctcatat
    49381 tatcagaccg ttttgtggag gtaccagaca tgtatggctg gacaaaatcc aatgttaaaa
    49441 cctttgctaa atggactgga atagacatca gctttaaagg aacagattct ggtcgtgtta
    49501 tgaaacaaag tgttgatgtt ggtaagtcct tgaaaaaaat aaaaaaaatg accattactt
    49561 taggagatta acatgttttt aacccttatt gcagcaatca tatcctttat ggtatctgct
    49621 ttcacaatgc cttattttat caaattctat caattgaaaa aaattggtgg gcaacaaatg
    49681 catgaagatg tcaagcaaca tttagctaaa gctggaacac cgactatggg gggaactgtt
    49741 tttcttttag tagctacggc agtatcgtta ttggttagtc tattttcgat caaaaacact
    49801 caaagtttag ctttgatttc aggcatttta tcaattgttg tcatttacgg gattatcgga
    49861 tttttggatg attttttaaa aatttttaaa caaatcaatg aaggattgac agccaaacaa
    49921 aagttagcct tgcagttggt tggaggattg atgttctatt ttttacatgt tagtccaagt
    49981 ggcatttcct ctattaatgt gtttggttat cagctgccgc tgggaatttt ctacctattc
    50041 tttgttttgt tttgggtggt tggtttttca aatgccgtta atttgacaga tggtatcgat
    50101 ggcttagctt ctatctcagt ggtgattagt ctagtgactt acggcgttat tgcctacgtt
    50161 cagagtcaat ttgatgtttt gttactgatt ggagcaatga ttggagcctt gcttggcttc
    50221 ttctgcttta atcacaaacc tgctaaagta tttatgggag atgtaggtag tttagccctt
    50281 ggagctatgt tggctgccat ttctattgcg cttcgtcaag aatggactct cctgattatt
    50341 gggatcgttt atgttcttga aacaagttct gtgatgttgc aagtgtccta tttcaagtac
    50401 accaagaaaa aatatggaga aggtcgtcgt atttttagaa tgacgccttt ccaccaccat
    50461 ttggaattgg gaggcttgtc tggtaaaggc aagaagtggt cagaatggca ggtagatgct
    50521 tttttgtggg gagtaggaag tctagctagt ttactagttt tagctatctt atacgtgttc
    50581 taagggggat tatcaagaat agtcaccaat agcattttga ttaacatcat caataagaga
    50641 gtggagatga cccacaaatg ctgattttgt gagtgttgtc ccactccttt tgcttgtctc
    50701 taacaataat gatataatat ggaagaaaac gaggtaaaac atgtcattta aagattatca
    50761 ttttaagcaa tacgttcagc aagcgctgga agaaattggc tttgtcaatc caacagaggt
    50821 ccagaagcgg ctgattccta ttgttaactc cggtcgtgac ttagttgggg aatcaaaaac
    50881 aggttcaggg aaaacccaca cctttttgtt gcccattttt gaaaaacttg atgaagctaa
    50941 agcagaagtg caagtcgtta ttacagcacc tagccgtgaa ttagcaactc aaatttttga
    51001 tgcttgtaaa cagattgcta agcacttcca agaagagatt cgtttggcca actatgttgg
    51061 aggcacagac aaactccgtc agattgaaaa actaaaaggc agccagccgc atattgtcat
    51121 cggaacccca ggtcgtattt atgacttggt caagtctggt gatttagcga ttcataaagc
    51181 aacgaccttt gttgtggatg aggccgatat gaccatggac atgggctttt tggatacggt
    51241 agataagatt gcagcttcgc ttccaaaatc tgttcagatt ttggtgttct cagcaactat
    51301 tccacagaaa ttacaaccct ttttgaaaaa atacttgacc aatcctgtta ttgaacaaat
    51361 caagacaaag accgttatcg cagataccat tgataactgg cttgtgtcga caaaagggcg
    51421 tgataagaat ggtcaactgt tagagattct caaaaccatg cagccttata tggctatgct
    51481 ttttgtcaat accaaggaac gcgcagatga tttgcatgcc tttttaacag ctaatggctt
    51541 aaaagtggct aagattcatg ggggcattcc tccaagagaa cgtaagcgca tcatgaacca
    51601 agtcaaaaaa ctggactttg aatacattgt tgccacagac ttagcagcgc gtgggattga
    51661 cattgaaggg gtcagccatg tgatcaatga cgccatccca caagacttgt ctttctttgt
    51721 ccatcgtgta ggccgaacag gtcgaaatgg tatggcagga actgccatta cactttacca
    51781 gccaagtgac gactcagata tcaaagagct ggaaaaaatg gggattgcct ttacaccgaa
    51841 ggtgctcaaa aatggagaat ttcaagatac ctatgaccgt gatcgccgtc aaaaccgtga
    51901 aaaagcctat caaaaattag acacagaaat gattggtttg gtcaaaaaga aaaagaaaaa
    51961 agtcaaacca ggttacaaga aaaaaattca gtgggcggtt gatgaaaaac gtcgcaaaga
    52021 acgtcgtgct gaaaatcgtg ctaagggccg cgctgagcgt aaggccaaaa aacaacattt
    52081 ttaagaacaa agagcaagca agtgatcaat tggcatctaa tcttgctttt tttacacctg
    52141 ctacttgtat tgtttaccat ttgttaacac aaaaagtgat catagctttt gcttattgct
    52201 ataatacgga tttactattt tacatgtggg tctaagagtg ttagaatatt aagacaaaca
    52261 aatacaaagg ggaaatacat gacatcagtg tttttaacca gtggttgggc tttttatgat
    52321 tacctcatct caccgattcc acatggaaaa ctttttagtt ggcatgctgt ttttgatgcc
    52381 attccaaata ttatccaacg gcttccaatt acgcttggtt tgacactatc aggagcaacc
    52441 tttggcttgg ttttggcttt gatttttgcc cttgtcaaaa ttaataaagt gaagctacta
    52501 taccctattc aagctatttt tgtgagtttc ttgcgaggaa ctcctatttt ggtacagttg
    52561 atgttgacct actacggtat tcccctcttt ctaaaatttc tcaatcagaa gtatggcttt
    52621 gattggaatg ttaatgcaat tccggcttct atttttgcca ttacagcctt tgcttttaat
    52681 gaagcagcct acgctagtga aactattcgg gcagccattt tatcagtaga tacaggtgaa
    52741 attgaagcag caaaaagtct aggcatgact tctgtgcagg tttaccgtcg tgtcattatt
    52801 cctaatgcaa ctgttgtggc tattccaacc ttgattaatg ggttaattgg cttaactaaa
    52861 ggaacatcgc ttgcctttaa tgcagggatt gtcgaaatgt ttgcccaggc tcaaattcta
    52921 ggcggatcag attaccgtta ttttgaacgt tatatctctg ttgctcttgt ctattggtct
    52981 atcagtattt tgatggagca agtaggtcgc ttgattgaaa acaagatggc catcaaagca
    53041 ccagaacaag ctagaaatga aaagttagga gaattgcgtt gatgattaca attagaaatt
    53101 tgagcaagac attctcaggg caaaaagttt tagatagttt ggctcttgat attgaaaaag
    53161 gtcaagtgat tgctttagtt ggtgcttcag gagcagggaa atcgaccttc cttcgtagcc
    53221 taaactattt ggaaaaacca gattcaggat ccattagcat tggtgacttt acggttgatt
    53281 ttgagaccat taccacagag caagtcctta ttttacgtcg gaaacttgcc atggtttttc
    53341 aacaatttaa tctttttgag cgtcgtacag cccttgagaa tgtcaaagaa ggtcttaagg
    53401 tggtcaaaaa actgtctgac caagaagcga ctaagctggc gcaagcagaa ttggctaagg
    53461 ttggtttagc agatcgaaaa catcattacc cacgtcattt atcaggggga caaaaacaac
    53521 gggtagcttt agcaagagct ctggctatga aaccagatgt tttattacta gatgagccga
    53581 cctcggctct tgacccggaa ttagttggcg aagtggaaaa gtctattact gatgctgcta
    53641 aatccggaca gaccatggtt ttagtgagtc atgacatgaa ttttgtttac caagtggcag
    53701 atagagtact ctttttagat caagggaaaa tcttggaaca aggaacacct gaagaagtct
    53761 ttagacatcc tcaaaaagaa cgaaccaaag aattttttgc aagctattca aaaacataca
    53821 tttaatgata ttcttttgtt ataataaaaa ggtctaggta ttcctgggct tttcttgtta
    53881 tgttttttag aaaaacttat gggaaagaaa taatgactag gtggaaatat gttagtagaa
    53941 atctttagcc tttatgtaaa aggtctcatt ctctcaagcc ttactatgat tttaatttgt
    54001 ttaatatgga ctttatggcg agcaaaaacc aaaagagata aaacattgat tgaaagacaa
    54061 gcttttcttt atgagatgct aatggttgct attttaacca tcccaatttt atcgtttgct
    54121 tttatgagta ttttggttgt cataaaatcc taatcattgc aagaagggat tatcattgct
    54181 aaaatattgg caagagtaga ttaaaggaga aaaatcatgt acgatacatt aattattggt
    54241 tcaggaccag caggaatgac tgccgcttta tacgctgcta gaagtaattt atctgttgcc
    54301 attattgaac aaggggcacc tggtggtcag atgaacaaca cttttgatat agagaattac
    54361 ccgggatatg atcatatatc tggaccagaa ttagctatga aaatgtacga acctttagag
    54421 aagtttaacg ttgaaaacat ttatggtatt gttcaaaaaa tagaaaattt tggtgactat
    54481 aagtgtgtgt tgactgaaga tgctagttat gaagctaaaa cggttattat tgcaacggga
    54541 gctaaatacc gtgtactagg tgttccaggt gaagaatact atactagtcg aggagtttct
    54601 tattgtgcag tatgtgatgg tgcgtttttc cgtgaccagg atcttttggt cgttggtggt
    54661 ggtgactcag cagttgaaga agctatttat cttactcagt ttgctaaaaa ggtaaccgtt
    54721 gttcatcgcc gtgatcaact aagagctcaa aaaatactgc aagatcgtgc ttttgccaat
    54781 gataaggtcg actttatctg ggattcagtc gtcaaagaaa ttcaaggcaa tgatattaag
    54841 gtctccaatg tcctaatcga aaatgtgaaa acaggccaag tcactgacca cgcttttgga
    54901 ggtgtgttta tctatgttgg tatgaatcct gtgacaggta tggtaaagga tctagaaatt
    54961 accgatcaag aaggttggat tatcacagat gatcatatga ggacaagtat tcctggtatt
    55021 tttgcgattg gagatgttcg tcaaaaagac cttcgccaaa tcacgacagc tgttggagat
    55081 ggtgcaattg cagggcaagg tgtttatcac taccttgaaa gtttcccttc ataaggttaa
    55141 aactccttca tttttccttg tctttccctt aaaaaagcta aattgcataa gacctttaag
    55201 aaatataaag aacagttttc cttaatatct ttcctagata aatgttgaaa attttctgaa
    55261 aaaaggatat aataaatagt attttagtga atggagttta gaatgtctat aaaagaacaa
    55321 acagacaata acgaactaga aaatggaatg gtaaggggct tagaaaatcg tcatgtgcag
    55381 ttaattgcga ttgcaggaac gattggaaca ggtctctttt taggggcagg acgctctatt
    55441 gccctaacag gaccatctat tatttttgtg tatatgatta caggtgcctt catgtttatg
    55501 atgatgcgtg ccatcggtga aatgctctat tatgatccag atcaacacac ctttatcaat
    55561 tttatttcca aatacattgg tccaggctgg ggttattttt cgggtctatc ctattggatt
    55621 tcccttattt ttattggaat ggcagaaatc acagcagtag gtgcttatgt gcaattttgg
    55681 ttcccaagct ggccagcctg gttgattcag ttggttttct tagtcttact tagttcgatc
    55741 aacttaattg ctgtgcgcgt ttttggggaa acagagtttt ggtttgccat gattaagatt
    55801 ttagctattt tagctttgat tgcaacagct attttcatgg tattgacagg ttttgaaact
    55861 cacacaggcc atgctagcct ttccaatatt tttgaccatt tttccatgtt cccaaatggg
    55921 aaactaaagt tctttatggc cttccaaatg gttttctttg cttatcaagc tatcgaattt
    55981 gtgggaatta ccacttctga gacggctaac ccaagaaaag ttctaccaaa ggctattcaa
    56041 gaaattccaa cccgtattgt gatcttttat gtgggagcct tggtctctat tatggcaatt
    56101 gtgccatggc atcagttacc agttgatgaa tctccttttg tgatggtgtt caaattgatt
    56161 ggtattaaat gggcagcagc cttgattaac tttgtggtct tgacatcagc agcgtcagcg
    56221 cttaactcaa ccctttattc aaccggtcgt catctctatc agattgctaa tgagactcca
    56281 aacgctttga ccaatcgctt aaagattaat actttatctc gacaaggggt gccaagtcgt
    56341 gccattattg cttcagcggt tgtggttggt atctcagctt tgattaatat cctaccagga
    56401 gttgcagatg cgttctcact cattacggcc tcctcatcag gtgtatatat tgctatctac
    56461 gccttaacca tgattgctca ctggaagtac cgtcaatcta aggactttat ggcagatggt
    56521 tatttgatgc caaaatataa agtgacaacg cctttgaccc ttgctttctt tgcttttgtc
    56581 tttatctctc ttttcttaca agaatccacc tatatcggcg ccattggagc aacgatttgg
    56641 attattgttt ttgggattta tagcaatgtc aaatttaaat aataattagc gatatagaac
    56701 tgaatccatt aactctttct tatttgagga tgtcatgatg gatcagttct ttttttactt
    56761 tagcgataac tcaagttatt tctaaaccct agccaaagag tcctttttaa aatatgttat
    56821 aatagtaaca ataattcaaa ggagtcccct ttatgtataa agatgatagt ttaactctcc
    56881 atactgattt gtatcagatt aatatgatgc aagtttattt tgagcagggc attcataatc
    56941 gtcatgcggt gtttgaagtc tatttccgca aggaaccttt taacaatggt tatgctgttt
    57001 ttgccggttt acagcgcatg gtggagtatc tagagcaatt tcaattttca gaaacagatt
    57061 tggcttacct tgaagaactg ggttatccag gaaacttttt gacctacctt aaagagttac
    57121 gattagaatt gaccatccgt tcagctaaag aaggagattt ggtttttgct aatgagccga
    57181 ttgtgcaagt agaaggccct ctaggtcagt gtcagttggt cgaaacagct cttttaaaca
    57241 ttgttaactt ccagaccttg attgctacaa aggctgctcg tattcgttct gtgattgagg
    57301 atgaacccct tttggaattt ggtacccgtc gtgcacagga actagatgct gcgatttggg
    57361 gaacccgcgc agcgatgatt ggtggagcag atgcaacaag taatgtgaga gcaggtaaac
    57421 gctttgatat ccctgtttca ggtacgcatg ctcatgcttt agtgcaagcc tatggtaatg
    57481 attatgatgc tttcatggct tatgctaaga cccataaaga ttgtgtattt cttgttgaca
    57541 cctatgacac acttaaagta ggtgtgccaa cagctatccg agtagccaaa gaaatgggtg
    57601 acaagattaa ttttctaggt gttcgattgg actctggtga cttggcttac ctctcaaaaa
    57661 cagtccgtca gcagttagac gacgctggtt ttaccgaggc taagatctat gcttctaatg
    57721 acctcgatga aaataccatc ctcaacttaa aaatgcaaaa ggccaaaatt gatgtttggg
    57781 gtgttgggac caaactgatc acagcctatg atcagcctgc cttaggtgct gtttataaga
    57841 ttgtctctat tgagcaagaa gatggtagca tgcgtgatac gatcaaatta tcaaataatg
    57901 cagaaaaagt atccacacca ggtaaaaaac aagtttggcg catcaccagt cgtgaaaaag
    57961 gcaaatcaga aggcgactat atcacgttta ctgatattaa tgttaatgaa ttaactgaga
    58021 ttgagatgtt ccatccgacc tatacctata ttaagaaaac cgtcagaaat tttgatgcaa
    58081 ttcctctctt agttgatatt tttgtcaagg gagaattagt ttaccagcta ccgaccttat
    58141 ctgaaatcaa gacttatgct aaaaaagaat ttgacaagct ttgggatgaa tacaagcgtg
    58201 tcttaaatcc tcaagactac ccagtagact tggcacgaga tgtgtggcaa aataagatga
    58261 ccttgattga taacattcgt aaagatactc atggaaagag tgaatgacat gacattacaa
    58321 gaagagatca ttcgacaatt aggcgtcaag gctagcattg atccacaaga agaaattcga
    58381 aaaaccgttg attttttaaa agcttatttg cgaaagcact catttttaaa aacttatgtc
    58441 ttaggcattt ctggaggtca ggattctacc ctcgcaggaa agctagccca gatggccatt
    58501 gcagagttac gggaggaaac aagtgataaa gcttaccaat ttatagctgt tcgcttgcct
    58561 tacggggttc aggctgatga agcagatgcg caaaaagcct tggcttttat tgcaccagat
    58621 cagaccctta ccattaatat caaggcagct gtggacggtc aagtagaggc tctacaagca
    58681 gcaggcgttg agatttctga ttttaacaaa ggtaatatta aagcacgtca gcgaatgata
    58741 agtcaatatg ccattgctgg ccaaatggct ggtgctgtta ttggaacgga tcatgcggca
    58801 gaaaacatca ctggtttctt tactaaattt ggtgatggtg gagcagatat tcttcccctc
    58861 tttaggctta acaaacgtca aggaaaggct cttttgaaag ttttaggagc agatgctgct
    58921 ttgtatgaaa aagttccaac tgctgattta gaagatcaaa aaccaggctt ggctgatgag
    58981 gttgccctag gggtgaccta tcaagacatc gacgactatt tggaagggaa gctgatttcc
    59041 aaagcagctc aagcaactat tgaaaaatgg tggcataaag gacaacacaa gcgtcacctg
    59101 ccaatcacca tttttgatga tttttggaaa taagaaatga tgttcaaaga gtaaaaaaag
    59161 aaaacaaaaa aagttaggtt atcctaactt ttttcttgaa gaaaatcaaa taaggtctat
    59221 aatagaagta atcctttaga atagaatggc tggataataa ccatcttgcc aatattattt
    59281 caggtgaagc ttatcttaat attttaggag gacaataatg tcagcattaa cagagacatt
    59341 tacagaacaa ttatttgctc attacgaagc caatgcaaaa ttttcagcta ttgagaatgc
    59401 cgttacgcat aatggtttgc taaaatcatt agaaacccgt caaagtgaag tggacaatga
    59461 ttttgtgttt tctattgatt taactaaaga taaggtatct aaccaaaagg catctggtcg
    59521 ctgctggatg tttgctgcct tgaacacttt ccgacacaaa ttaatcacag agtttaaatt
    59581 agaaaatttt gaactgtcac aagcccatac tttcttttgg gataagtatg aaaaagctaa
    59641 ctggtttatg gagcaggtca ttgctacagc tgaccaagaa ttaaccagtc gtaaagttaa
    59701 gtttctcttg gatgttccac aacaagatgg tggccagtgg gacatggtgg tttccttgtt
    59761 tgagaagtat ggagtggtgc caaaatctgt ttacccagaa tcaatctcat caagtaacag
    59821 tcgtgaatta aaccagtatt tgaataaatt attgcgccaa gatgctcaaa ttcttcgtga
    59881 cctgattgca tcaggtgcta aggcagacca agttgaggat cgaaaagcag agcttcttca
    59941 ggaaattttt aacttcttgg ccatgaccct tggtttacca cctcgtcatt ttgattttgc
    60001 ctaccgtgat aaagatgacc actatcatgt tgagaaaggt ttgacaccgc aagcgtttta
    60061 cgacaaattt gttggcttaa agctttctga ttatgtgtct gttattaatg caccaactgc
    60121 tgataagcct tatggcaaat catacactgt tgagatgcta ggaaatgtgg tcggtagtcg
    60181 tgaggttcgc tacctcaacc ttgatatgaa acgtttcaaa gaactagcta tcaagcagat
    60241 gcaggctggt gagtccgttt ggtttggctc tgatgtgggt caagtttcag atcgtcaaaa
    60301 agggattttg gcaactaata cttacgattt tgaagctagt atggatatca atcttagtca
    60361 agataaggct ggacgtttgg attatagtga aagtttgatg acacatgcta tggttttaac
    60421 aggtgttgac ttagatgaaa caggaaaacc gttaaaatgg aaggttgaaa attcatgggg
    60481 tgagaaggtt ggtgacaaag gttactttgt tgcttcagat gcttggatgg atgaatatac
    60541 ctatcaaatt gttgtccgta aagaattctt aacagctgac gagttagcgg cttatgaaaa
    60601 agagccacaa gtactagctc cttgggatcc aatgggtgct ttagcacaat gctaattcct
    60661 ttctttgcga ctgtatgaaa tctaaaggct ggacaaaacg aacagcaaac ggattggact
    60721 ccgcataaga aagtgaacat gaaaaaaatt attatgctaa gaaaatgaca tatgaaaaaa
    60781 cacgtcttcg gtatatggaa acgtgttttt tcatgtttac tctcttatgg cattagttgc
    60841 tatttataac aattttaaat aagtctattt tgttttaggt taacgtttga aaaatcagtt
    60901 gctaattccc ttaacgttgt accttttctt taatacgatt aaagaattct gttttttgtt
    60961 ttatattgta tgtggtaagt catttgtgtc atataaaaac gagtcctcac agaacgtgga
    61021 ctcgcttttt atttagatta tcacttaagg tagatattaa aagtaactaa atggatatgt
    61081 aataaaaggt aggtacagac aataagtaga gttagagatt aatgagttgt ctttttatca
    61141 tcggtagatg gatgattgct attgttgtct gtattggttg aattacttat atcattgcta
    61201 ctactagtat catctgaagt catttggcta gaagctgttg tcgtgttatt gctgtaaaga
    61261 ttgttgtaaa ccgaattagt atagtcggtg ttgctcgcat aggttccgct taagtagagg
    61321 aatccaccac tgcgataaag accatttggc atcgtccaat cttcactgta accatttgtt
    61381 aagtaagtca tcatgctacg ataaacgtca gatgcaatct ctagacttga tccgtatact
    61441 ggggtcaagc ggtttttgta acctgtccaa acagccatgg cgtagcgctt agtaaagcca
    61501 acaaagtttt cgtctggcgc taatgtacca acataatctg gataaaggcc gtatttttca
    61561 ccaattttag ctagttcctc atcagtgtag ttagaagtcc ctgttttacc agcttgcgca
    61621 acaccaggaa tggcagcagc agtacctgta ccataagtga gaacagtttt taacatatct
    61681 gtcatcatat aggccgtggt ttctttcatg gcacgttttc ctttttcatc aaaagtttta
    61741 cttgtaccat cactaaattc cactttattg acataccttg gtttatgata aataccacca
    61801 tttgcaaaag cagcgtatgc agcggccatt ttttcactac ttgcaccata ttttttatct
    61861 gagctactgt tattacttga gatagcgttt gagtagtgca tttcgggata gttaatacct
    61921 aaactgctta agaaagatcg agcatagtca agtcctgcgg cttccagtgc tcggactgct
    61981 gggacatttc gcgatagcat aatagcagct tggattgtca tccatccgtt atatctaagg
    62041 tcccagttaa acaattgggt agtggttcca ggccaataat agactgagtc attagtcgac
    62101 tgagcagtag aagtataaac accagattca atagcaggag cataggctgt gattggcttc
    62161 atggtagaac cccagtcacg atcagttaaa acagcttggt tagtcccaaa tgaaacattt
    62221 tcatcttgat tacgtccgcc aagctgagca ataacatgac catttgtcac atcaacaatc
    62281 gttgaagcaa cttggaaatc ttggtctgga tagtaaacat aatcaccaga atgataaata
    62341 ttataaagag tctgctgcgc atcggggata atattggtat aaacttttaa accagcggta
    62401 aaaatatctt tattcgtttc ttttttgact tcttcaataa cttgttttag ataattatcc
    62461 atatattttg gataagttga gcgctgttgg agtgattgta gaccttcagc gacgggagtt
    62521 gcgatggcag tttcatattc tgctttcgtc agatgttttt ccatgtacat ctgttgcaac
    62581 acgacgttac ggcgattttg agcagcttca ggatgaaggt aaggatcata ttgactagga
    62641 gcttgaggga ttccagccaa tagggctagt tgggcataag ataaatcctt aagatccttg
    62701 ccataataag acttagcggc tgtcagcata ccatagttgc cattacccat atatactttg
    62761 ttgatgtaaa aagtcaggat ttcttgttta gtatattttc gctccatttg aagagcaagc
    62821 caaacttctt gagccttacg ttttaaggtt tgatcagatt cattagtaga aaaataggct
    62881 agtttaatga gttgctgatc aagcgttgac cccccttgag tggtctgact cgttagatta
    62941 tgaaaggcag caccaaaaat acgataaaga tctactccac gatggttaaa gaaacgttta
    63001 tcttcaattg aggtaatagc attaactaga ttaataggga tactatcagc tgttacattt
    63061 tcacgctttt cagaacccaa atcagcaatc agattgttat taccgtcata aaccaagcta
    63121 gagtttgttg attttaactg ggcttctgac agtttcggag cactgctaat gtagaaggta
    63181 aacaaaagac caccaataat aataacaagg ataataaggc taagaattgc acttaataca
    63241 tactttagcc acttaaggat ttttggattt ttaattgtaa tcaccgccta aaaaattttg
    63301 ttcaattatg tttaaataag ggacttgtgg aaaggctcca aatgctacct taaagccatt
    63361 ttttctgata taatcaatgg gcatcgattt actgccatta tctatctgat aaaatgaaat
    63421 caaggcttga gcaggtaggt agtaagtctc cttcagtgtg gaaaagtgaa gaaggacaaa
    63481 gcagattcct ttttgatgta aaacgcaagc catatgttca atctgatgta aatggaaatt
    63541 tttcatgggc atagctgttt tttgcctggt ttcttttgct tcaaaatcaa cataatgtcc
    63601 tttataaacg ccgcaatagt cagtcgttga agcttgtcta aagtacgctt caacgatttt
    63661 tgcccgactg cgtttagggt aatccacttt gacaatttgg acaggagtag gttttttgtg
    63721 aatgacagct atttgacgag ataaataata atcatttgtc gcgttgatag cagcttcaaa
    63781 ggacatacct ctattggcga agtccacctt attttgcttt gtttgctttt gaacggaact
    63841 tactttttgt cgaataagat tatgaggata attaaccatt gttctcctaa tattatctag
    63901 ttaatgctaa aaatttaagc acttttatta taacatattt tttgaaagga taacacctaa
    63961 tgactgcgat tctaattaca ggttaccgaa gctttgaaat agggattttt gaccataaag
    64021 acccacgagt ttcaattatt aaacaggcta ttcgtaaaga tttaataggt tatttggaaa
    64081 atggtgtgga ttggtttatc tttacaggga acttgggttt tgaacaatgg gctttggaag
    64141 tagctaatga gctaaaagag gagtatccct tgcagatagc tactatattc ttgtttgaaa
    64201 cgcatggaga taggtggaat gaaaaaaatc aaaaagtttt aagtcagttt agggctgttg
    64261 actttgtaaa atattacttt ccaaattacg aacaaccgac gcaatttagt caatattatc
    64321 agtttttatt agaaaagaca gaaggggctt atgtttttta tgacacagaa aacgagacca
    64381 acttaaaata ttttctcaag aaagcaaaag atatgccgca ttatcagttg ctattgctaa
    64441 cctttgatcg gctaaatgat atgtcacaaa gctagcattg tatttttagg ggtattggta
    64501 tttaaatact tgctttttga actatttttc tgtatcatta ataataatac tgaaatataa
    64561 ttttggtaaa aagattgacc gttcggagag taaagaatga caagtattat ttatagtcct
    64621 aaagatattt ttgagcaaga atttaagact agtatgcgag ggtttgataa gaaagaagtt
    64681 gatgaatttc ttgataatgt aattaaagat tatgaaaatt tcaatgctca aattgaagct
    64741 ttaaaagctg agaacgaagc cttaaaaaag gccaagtttc aagctagaaa tacagtttca
    64801 gcgactgttc aacaaccagt accgcaaccg actcgtgttg ctcaatcagc tactaatttt
    64861 gacatcttga aacgtattag taaattggaa aaagaggttt ttggtaaaca aattattgag
    64921 taatgaaaac tgtgcaattt ttggataatc gcgtagtatt ttaatactat gaggaaagtc
    64981 catgctagca ctggctgtga tgccagtagt gattgtgcta ggcgaacaca taagcctagg
    65041 gatgtgcata cacattacgg cgaaggaaat ggctaagtcg tttgatatgc caaagtactt
    65101 ctgaaagtgc cacagtgacg tagtttttat ggaaacgtaa aaaatggaac gcggtaaacc
    65161 cctcaagcta gcaacccaaa ctttggtagg ggcatgggat agttggaaac gaacaagcta
    65221 tcctgactgt caacagacgg tagacagatg attatcgaag gaaataattc ctagttattt
    65281 ccggaacaaa acatggctta tagaaaattg cataataggt taagctagca cttgctagct
    65341 tttatttgat ttaaaagaaa gagatatatg aaagaaacat ttagattagt cgcaactgca
    65401 gccgcaggtt tagaagctgt cgttggtaaa gaagtcagag cactaggttt tgattgtcag
    65461 gttgaaaatg ggaaagttta ttttgaaggt gatgtagaag cgattgttaa aacaaatctt
    65521 tggttacgtg cagcagatcg tatcaaaatt atcgtaggac aatttcctgc tagaaccttt
    65581 gaagaattat tccaaggtgt ctttgccttg gattggggaa attatttacc gctaggagct
    65641 aagtttccaa tctcaaaagc caagtgtgtc aagtcaaagc tacacaatga gccaagtgtt
    65701 caggcgatta ccaaaaaagc tgttgttaag aaactgcaaa aacatttcca caggccagaa
    65761 ggagttcctc ttcaagaagt tgggtcaacg tttaacattg aggtatctat tttaaaagac
    65821 caagccacta ttatgattga cacgacagga tctagcctat ttaaacgagg gtaccgcgtt
    65881 caaaagggtg gggctcctat taaagaaaat atggcagctg ctatcttggc cttatctaac
    65941 tggtttccag ataaacctct ggttgatccc acctgtggat cgggaacgtt ttgcatcgag
    66001 gcagccctga ttgggatgaa tattgctcca ggatttaacc gttcgtttgc ttttgaagaa
    66061 tggagctggg tagataaaga catggtgcaa caagttcgtg atgatgccga acaagaagct
    66121 aattatgaaa tcgaattaga tatttctggc tttgatatcg atggccgcat gattgagatt
    66181 gctaaaagta atgctgaaga agctggttta tctgatgtga ttacctttaa gcaaatgcgt
    66241 ctgcaagact ttcgaacaga caaggttaat ggtgtagtga tatcaaatcc tccttatgga
    66301 gaacggttgc ttgatgacaa agcggttgac atcttgtata atgaaatggg acaaaccttt
    66361 gcaccattga aaacatggag caaatttatc cttacaagtg atgaactttt tgaactaaaa
    66421 tacgggcaaa aagctgataa aaaacgtaaa ctttataacg gcaccctaaa agttgatttg
    66481 taccaatttt atggggaaag ggtaaagcga catctgatat agagaggtag taagcgtgtc
    66541 agaagaaagc aaagaagtcg aagtaacaaa agaatcgcaa acattggggc ttaatgaagc
    66601 caagtcaatg acaattggtg aagctgtacg caaacagtca gagataaaag caggtgttac
    66661 taaggatgat agtattttag ataagtacat taagcaacac cgtgacgaag tttcttctca
    66721 aaagtttgat gctaagtata cagagcttga tacagctagt ttagataact ttatcaaaaa
    66781 acaaagagaa gcattgagta aagccggtct tgttgatgac gaacctgtgt ctgcggagtc
    66841 tgctgagcaa gatagcactt tggttgaaga agtagctgaa gacttagctc caatggagac
    66901 tactgcggtg gtaactggga ttccggtgga agcgacagtc cctgtgttag atctagatcc
    66961 atcagagagg gtcattccag aaccacagat gaccaaggaa gagccaaaaa gagatcagtt
    67021 tctttctgaa gatagccacc atccagccaa acaaaacacg aaaaaaggtt ggttaatagc
    67081 tttatttcta ctattattgg ccattcttgc agttgtattt ggttggaacc atttcttgag
    67141 acaagacagt ggtaaaacaa ctcaaacagc aagtaaacag acaaagacct ctttgcagac
    67201 cgacagtgct aaaaaagcaa cgcgcttgaa ggctgctgct aaagctttcg aaaaacaata
    67261 tggtactttt tatacagatg ccacaaagag caaattaaaa aatagtgcct ttgctacttt
    67321 gccagattta gaagctgcct tgaaagcttt ggaaggctct gcttactatg ataaggcgaa
    67381 agcaaaagtc gattccctta agaaagctat tgctgctatt acagcggtta acggtaaatt
    67441 tgtttccgat gttgttgtag atggtgaaaa ggtttcggct gaagtcaagg ctgatgctaa
    67501 ttttgatgat ttatcaagtg caaccttgac aacaggaaat gcaaatcttg atgctgtctt
    67561 gcaggcaagc atcacagaag gtcgtcagca actagccagc aaggctgagg ccgctaaagc
    67621 agctaatgag caagcagccc aggctcaagc agctcaagga caaagtacat cagtagcacc
    67681 atctggctat ggcttgacaa gttatgaccc tgcaagctta caacggcatt tatcacgtgt
    67741 accttacaat caagatgtta ttgctgacag agctaaccca tcttgggcat ttaatccagg
    67801 agttcttgaa aaaattgtag caacctcaca agctagaggt tatatttcag gctaccaata
    67861 tattttagag ccggttaata ttattaatgg caatggttac tataatatgt ttaagcctga
    67921 tggcacttat ctgttctcta ttaattgtaa gacgggttat tttgtaggaa atggcaaagg
    67981 ttatgctgat gctttagatt actgagaaaa tcaaaaaaaa ctgagataag gcaacttgtc
    68041 tcagtttttt gattaataaa gtatgttgtc ttaaaatctg cagatagact tgttttttgt
    68101 aacaagtcaa ataggtcagt aggattgtta tgtttgacaa cacactagta tcagatgaca
    68161 tggcgactaa aaggatcgtc tgaaatccct tgatcaataa tcaattgagc ccattctttg
    68221 gcggcaaaga ggctatgatc cttatagttt ccacaggatt caagagttgt tccaggaaca
    68281 tcttcccaag taatcccagt tgcaatttct tctaggctgg atttaatcac cttggcaata
    68341 tcagtagaac tgtgttttcc ccacatgata aggtgaaaac ctgttcgaca gccaaaagga
    68401 gagcaatcaa tcatcccatc aatgcgttgg cggatgagct tggcaagtaa gtgttcaatg
    68461 gtatgcaaac cggctgtttc aatagaattt tggttaggct gcactaggcg aacatcaaaa
    68521 tttgtaatac gatcgccctt gggtccaaat tcttcagaaa taagacgaac ataaggggcc
    68581 tttacaatag tatgatctag ctcaaaactt tcgacgataa cttcttttgt catttcaatc
    68641 tcctttgttt tacactttat cataacacaa atagtgagaa agtggctact tgtggaggag
    68701 aaaaggctaa gaaaacaaat caaaggctat cacaatgacc tgtgcgataa aagtacttac
    68761 agaaacagta ataaaagttt ctttaaaagc atttttgtgg ttaagtcctg tcaatgctaa
    68821 gaaggtgaga aagacaccac tttggggtac aatggtaaag atattcgaag caatagtggc
    68881 aacacggtga atcatttcgg gattaaggcc ttgatctaaa taatactggg caaaattagg
    68941 catgacaatt cctagagcac ctgaagaaga accggtaata gctgacatag aggaagtcaa
    69001 cacggctaaa ctaataagag gattaccggg aatattcaaa atgagatctg agaagaatgt
    69061 gaaacctggg gcaatcatca caacggctcc gaaggcaact gcagatgctg tcgcaaaaat
    69121 aggtgcaata gagctgctag ctcctaaatt aaagacagca atcttattag gaataaactg
    69181 tctaaagagc cagcttgcgg tgagaatggc agctaaaagt gctatgaaaa taatgttctt
    69241 cttaaaaaaa tcgtttccga aaaggctacc ggttagggcg ataataatga gcaacaataa
    69301 aggtaagata ctggcaagga aatgtgggag attttttgtt ttcacttgta tctcattgtc
    69361 aaaagcataa gtggcataag tttcacccct tgccatactt tttgctagac aatatttcat
    69421 ataaaagagc ccaaaagtca cgcagccaat tgagccaacg atactcggaa tggcggcagc
    69481 agttagtgag gtgtccaagt attgaatagg aataacattt tgaatggcag gtgttccggg
    69541 taaaatcgtc atggtaaaag ttgcaatacc gagccaaaga ggaacttgaa ttaagttcta
    69601 agccaagtcc atttttttga ataggcttcg tgcaagcggc aagacagcaa acatgacaac
    69661 aaagaggctg attcccccat aagttaatat agcactgatc agaaaaatag caacgagtac
    69721 tttataagga ctatcatgac caactttttt taaaatataa tctgcaatag aagtggttgc
    69781 accgcttgtt tccattaatt tggctaagat agaacctaat aaaaagatag caaaataatt
    69841 gagaatataa gtggagagtg cccccataaa ttggttgggt tcttttccaa gtaaggttgt
    69901 ggttggatcc atttgattaa accaaatgac caagctggta gcaagaggcg cagcgatgat
    69961 aatgttaact tctttgacag acaggtaaat aataacaata acacctacca agacccccag
    70021 agaaccaata atttccatag ttttatgacc tccttattag aaaataaaga gacagatcac
    70081 aagatacagc ctctttatct ctccaaattt attgagcagt gtagccaccg tctaagatac
    70141 aggcttgacc tgtaacacct tttgccttat cactggcaag gaaagacaca tagtctgcaa
    70201 tttcttgaac gtcaatgagg cgtttttgag gaacaagtgg gtatagcact tcttcaagaa
    70261 cattttcaag gggaatacct cttgttttgg aaaggtcttc aaactggcca cgaaccagtg
    70321 gtgtgtcaac atatccagga caaatggcat tgaccgtaat gcctgaatca gcagcttcta
    70381 aggcagttac tttggtcaga ccgatcaagc cgtgcttagc tgaattgtag gcggatttgc
    70441 cagcaaaacc aatgacacca ttgatagaag ccatattaat aatacgaccg tgtttttgag
    70501 ctttcattgt aggaaaagca cgtttaatgg caataaatgg tgctgtcaac atgattttaa
    70561 tcatgaattc aaatttttca gttggaaaat cttcaatcat ggcaacatgt tgaaggcctg
    70621 cgttgttaac caaaatatca atacgaccat atttttcaac aaccgtatca atagcagcat
    70681 tgatggctac ttctttggtg acatcgcaca caacactaaa agcgtcatag ccttctttag
    70741 tatagtcagc aacaacctga tccagttttt cttgattaat gtcagaaaaa acaaccgttt
    70801 taccttcttt taagaaggta aaacggttgt tttccaattc cgcttgcagc accagtgata
    70861 aatgcaactt ctttagtatg tgtcatgata ctatcctcgt ctttcttttg gttagtcttt
    70921 agtttgtttt atcaattagg gtaacttcgg taacgttttg cacgtcttca aaggtgtaat
    70981 ctgggttaat ttcaagggcg catagacctt catcttgata ttcaaagact cccatttcag
    71041 taatgattag gttaacaaca ttttgagcag tcagaggaag ggtgcatttg tctaaaattt
    71101 ttgctttgcc tttattggta tgctccattg caacaatgac ttttttagca cctaccaata
    71161 agtccatggc ccctcccatc ccaggaacca tttttccagg aatcaggtaa ttagcaatgc
    71221 tagcttcttt atcaacttgt aaggcaccta agacggttgc ggccacatgg ccgcctcgaa
    71281 taataccaaa ggaagttgag ctatcgaaga aagcccctcc aggagcaata ctaacaggtt
    71341 gcccaccagc attaacaata ctaggatcgt agtgatcatc tgttaatcca gtgagaccga
    71401 caaaaccatt ttcggactgc agggtaatgg ttacaccttc aggaatatag ttggctactt
    71461 ttgttggcaa acctatgcct aagttgacca gtgtattgtg ttctaattct tgtgctacac
    71521 gctttgcaat acgtgtttga atctcttctt ttgaaagaac agtagctgat gttcccatgt
    71581 taggctcctt tcactaagta gttaacaaaa atgccaggag tatggacaaa attaggatcc
    71641 atttgcccca cgtcaacaac ttctcttgct tcaacaatgg tggttttcgc attagcagcc
    71701 atcacatgat taaagttatt ttcagaacct gcgtattgca aattgccatt tttatctgcc
    71761 ttgttagcaa aaataagtgc gatatcagct ttcaaaggtt tttcaagaag gtagtctttg
    71821 ccgtcaatag taatgacttc ttttcctttg gcaacttcgg ttcctattcc agtaggtgtt
    71881 aagaaaccgc ctagcccaaa accgccactg cggatgcgct cagctaatgt tccctgaggt
    71941 acaagatcaa ttacagtctc gccctcagtc atttgtcggc ccgcttcacg gttgagaccg
    72001 atatgtgatg caataatagt tgaaaactgt ttattggcaa ccatcttacc aacgcctttg
    72061 tcaggaaaac cggcatcgtt acagattaaa gtgaggtctt tgacaccttt ttccacaagt
    72121 gcatcaatta atttttctgg tgtgccattt gtcatgaagc caccaaccat aatggtatca
    72181 ccatctttaa cgtgagctac cgcttctttt attgtgattt ctttacacat tttttctacc
    72241 aactttcacg agactaagta ttgtttttca cgataacagc agttccttgt cctccaccaa
    72301 tgcaaagtgt tgctagtcct ctggttactt ggcgtttttt catttcgtgg attaatgtga
    72361 ctagaatgcg agcgccactg gcaccaatag gatgacctaa agcgatggct cctccattaa
    72421 cattcacgat ttctgggttt aactttaaat cttttaaaac cgccaaggac tgtgctgcaa
    72481 atgcttcatt ggactccact aagtccaaat cagaaattgt taatccagct ttcttaaggg
    72541 ctttttgagt ggcaggaatt ggtccagttc ccatcagttc aggtgcaaca cctgcactag
    72601 catagctttc aatagtgact aaaggtgtta accctaattc aagagctttt tcttcagtca
    72661 ttaacatcaa taaggctgca ccatcgttta ttcctgaggc attcccagca gtaacggttc
    72721 cttctttagg gaggaaagca ggacgtaatt gctgtaattt ttcaaggctt gtgtcaactt
    72781 ttgggtattc atccgtcgta acaattaaag gatctttgcg tcgttgaggc acagagacag
    72841 ggacaatttc gtcagcaaaa cgatgatttt ctaatgccct agcagctttt tcttggcttt
    72901 ctaaagcaaa ttgatcttgc tcttctcttg agatgccata tttctgagca acgttttcag
    72961 cagtaatacc catgtggtaa ttgttaaaag catctgttaa gccatcatga acaagagtat
    73021 caatcaattg actattacca agacgttgac caaagcggtg ctgagttgag acataagctg
    73081 cttgtgacat gttttcgaca ccaccagcaa taacgatatc agcgtcacca agcatgatgc
    73141 tttgcgcagc taattggatt gctttaagtc cagaaccgca aaccatgtct aatgtgaaag
    73201 ctgttttatc ttcaggaatt cctgagtgga ctgcaacttg tcttgccaca ttttgtccta
    73261 ggcctgcatg aaggacgttt cctaggatga cttcatcaac catttcaggc tttacttgcg
    73321 ttttatcaag gatttttttc agtacagttg ttccaagttc aacagcagag acacttttaa
    73381 aggagcctcc aaaagctccg ataggagttc gtaaagcttc aacgataact atatttttca
    73441 taaaataaac atcctttcag ttttagaaag cgctttccat gtctctaaga taacaagaaa
    73501 acgctaaaaa taaaaatata gaatttttat aagcgtataa atttttttga tagagttggc
    73561 aatagcattt aacatctact tgtgtcataa tataggtaat tataccggaa aaagcaattg
    73621 ataagacaag gagaatccta tttggatatt aaacaaattc gttattttat cgccatcgtt
    73681 gaaaatcatt ttaacttgag ccaggcggcg gaactgttgt atgtgtcgca gccgacactt
    73741 agcatgatga tcaatgattt tgaaaaaagg gaaaatgtta agctcttcaa acgaaaaaga
    73801 ggtcgtatta ttgggttaac ctatcttggt gataattatt ataaggacgc tcaaaaggtt
    73861 ctcagtctct atgatgacat gtttttgaaa ttgcatgatc acagtaaagg gttaaaggga
    73921 agtatcaata ttggtattcc tcctcttatt ttatcagttg ttttctcaga agtaatgtca
    73981 aaactgattt tagaaaatcc ggggattcaa ttcaatgtta aggagattgg tgcttaccaa
    74041 ctaaaaaatg aactactcgt gggaaatgtt gacgtcgcag tcttgttatc cccaacaggc
    74101 atcgctgata atttggttga gacttatgaa atccagagat cagaattatc tgtatgcctg
    74161 tctccacgcc atcggttagc ttccaaaaaa gttattcagt ggaaagattt aacagatgaa
    74221 caacttgccc tatttgatcc tagtttcatg gttcaccatt tagtgcttga agcttgcgag
    74281 cgtcaccaag tcagacctaa tattattttg acgtcctctt cctgggattt tatgcttaat
    74341 tctaccaaga ttaatcacaa cgtcttaact atttgtccca aacctattac agaattatac
    74401 caattaaagg acattaagtg tattccaatg gaacgtccca tttcttggcg tgttgtctta
    74461 acacgcctcc ggaaaaaaag ttattctgaa atagaagcct acatcatgga cgacttgcta
    74521 cagtcttttt ttaagtagga ttctcaacca cttggcccag atgttaattt ttttaaaaat
    74581 gaattggaat tgtgtaaacg ctcaatttat gataaactaa gaggtggctt tctattaatg
    74641 aaatctagaa tatattgagg tacaaacatg gttaatatta ttttattaat tgtttctgcc
    74701 ctcattggtt taatattagg ttatgcactt atttcgatta gactcaagtc tgcgaaggaa
    74761 gctgcagagt tgactctttt aaacgctgaa caagaagctg ttgatattcg tggcaaagca
    74821 gaagtagatg ctgaacacat caaaaaaaca gctaaacgtg aaagtaaagc aaatcgtaaa
    74881 gaattacttt tagaagcaaa agaagaggca agaaaatatc gtgaagagat tgaacaagaa
    74941 tttaagtctg aaagacaaga gcttaaacaa ctcgagacac gcttagcgga gcgctcctta
    75001 actcttgacc gtaaagatga aaacctatca agtaaagaaa aggtactaga tagtaaagaa
    75061 caaagtctga ccgataaatc taaacacatt gatgagcggc aacttcaagt ggaaaaactt
    75121 gaagaggaga aaaaagcaga actggaaaaa gttgctgcga tgacgattgc agaagcgcgt
    75181 gaagtgattt taatggagac ggaaaacaaa ctgacccatg aaattgcgac gcgcattcga
    75241 gatgccgaac gtgacatcaa ggaccgaaca gttaaaacag ccaaggactt gttagcgcaa
    75301 gccatgcaac gccttgctgg tgagtatgtg actgaacaaa ctattaccag tgtccatctc
    75361 ccagacgaca acatgaaggg ccgaattatt ggacgtgaag gccgtaatat tcgtacttta
    75421 gagagcttga ctggcattga cgttattatt gacgatactc ctgaagttgt tatcttgtca
    75481 ggatttgatc ctattcgacg tgaaattgct cgtatgacct tggaatctct gattgctgat
    75541 ggtcgcatcc atccagctcg tatcgaggaa ttggttgaga aaaatcgtct tgaaatggat
    75601 aatcgtattc gtgagtacgg tgaagctgca gcctatgaga ttggtgcacc aaaccttcat
    75661 cctgatttga ttaaaatcat gggacgcctg caattccgta cctcgtttgg tcaaaatgtc
    75721 ctacgtcact ctgttgaggt tggtaagtta gctggtattt tagctggtga gttaggtgaa
    75781 aatgttgctc ttgcccgccg tgctggtttc ttgcatgata tgggtaaagc tattgaccgt
    75841 gaggttgaag gtagtcacgt tgagattgga atggaatttg cacgtaaata caaagaacat
    75901 ccagttgttg tcaacactat tgctagccac cacggagatg tggagccaga ttctgtgatc
    75961 gctgtgctag tagctgcagc agacgctctc agttcggctc gcccaggcgc tcgtaatgag
    76021 tcaatggaga attacatcaa gcgtcttcgt gatttagaag aaatcgcgac aagttttgat
    76081 ggggtacaaa atagttttgc tctacaagct ggacgtgaga ttcgtattat ggttcaacct
    76141 gaaaaaattt cagatgatca ggttgtcatt ttgtcgcata aagtaagaga aaaaattgaa
    76201 aacaatctag attacccagg aaatattaaa gtaactgtta ttcgtgagat gagagcggtt
    76261 gattatgcca agtagaaaaa gaggaattat cctctttttc tttatgactt gcctttgaaa
    76321 atgttcttga aaaagactag cctttttgat aaactattaa aaagcaacta ataaggagaa
    76381 tacaatgtct gaacgcggtt tgttaatcgt cttttctggc ccttcaggtg tcggaaaagg
    76441 aacggtaaga caagaaattt tttcaacgcc agatcataaa tttgaatatt cggtttcaat
    76501 gacgactcgt ccgcaaagac cgggcgaggt tgatggggtt gattatttct tccgcacacg
    76561 tgaagaattt gaagaactca ttaaaactgg tcaaatgcta gagtatgctg aatatgttgg
    76621 taattactat ggaacccctt taacttatgt taatgagact cttgataagg gaattgatgt
    76681 ctttttagaa attgaagttc aaggagctct tcaggttaag tcaaaagtac cagatggtgt
    76741 ttttgttttt ttaacaccac cagatttaga tgaattagaa gaccgcctgg ttggtcgtgg
    76801 tacagatagt caagaagtca tagctcaacg tattgaaaga gcaaaagaag aaattgccct
    76861 tatgcgcgaa tacgattatg cagttgttaa tgatgaagtt gccttggcag ctgagcgggt
    76921 taagcgtatc attgagacag aacactttcg tgtggagcgc gtgattggtc gttatgataa
    76981 aatgattaaa ataacaaaaa atccctttaa agctaaatag aaaagagtaa gcattatgat
    77041 gttaaaacct tccattgata ccttattaga taaagtacca tctaagtatt cacttgtgat
    77101 tttacaagcc aaacgtgccc atgaactaga agctggtgcc actccaactc aagaatttaa
    77161 atcagtgaag tcaacacttc aggcacttga agaaattgaa tcaggaaacg ttgtgattca
    77221 cccagatcct tcagctaaac gtgaagctgt ccgtgctaag attgaggcag aacgcttggc
    77281 caaagaggaa gaggaacgta aaatcaaaga acaaattgcc aaagaaaaag aagaagaagg
    77341 agaaaaaatc taaggtttca ggaatcttag attttttcat atttagtacg atgtccatga
    77401 cagaaaagga gaagcaaatg gaaaaagtag ctcatgtgat tgtagatatt ccattgatgc
    77461 aaacggacaa gccattttct tatggtattc caaaagagtt agtttctctc gtgcagcttg
    77521 gttctcgtgt gcatgttcct tttggaaaag ggaatcgttt gttgcaaggc tttgttattg
    77581 gttttggcca ggaagattca agctctctta aactgattca gaccgtttta gatcctgagc
    77641 cagtcctaaa ccaagaacaa ttgacccttg ctgatcagct tcgaaaaaca gttttttctt
    77701 ataaaatcac gctcttaaaa gcaatgattc ctaatcttct taactctaac tatgataaag
    77761 tgctaaggcc agagtcaggt ctaaaaaagc ctgatagaga ctttcttttt gaaggaaaac
    77821 catctgtctt atattctacc ttagaccgcg agaaggaaaa aatagctctt aaggccatac
    77881 aagctgggca tattactgtt agttatttag ccaaagacaa gaaaaaccta aagacagaaa
    77941 aatattatca cgtcgactta gacgccttag cggtacatcc catctctagt cgtgccaaaa
    78001 agcgtcaatt attaaaagac tatctgctaa cccatacgaa ggaagctaaa cttgcaacgc
    78061 tttatcaagc cttttctcga gatgtggtgg cttactttgt gacaaatcac ttaattcgta
    78121 ttgacgaacg tcaaattgat cgcagtgagc cctattttga ccagatcaaa ccatcttctt
    78181 ttttaacctt aaatgaacag caagcgtcag ctgttactga aattgttgag caaattggga
    78241 aaccgagtaa accttttttg attgaaggga ttacaggttc gggtaagaca gaagtttatc
    78301 ttcatatcat cgaagcagtt ttaaaacagg ataagacagc cattgtttta gtaccagaaa
    78361 tttcgttgac tcctcagatg acctctcgtt ttatttcccg tttcggaaag caagtggcta
    78421 ttatgcattc aggcttatca gatggtgaaa agtttgatga gtggcgcaag ataaaaacag
    78481 gacaggctaa ggttgtggtt ggtgcccgct ctgctatttt ctccccacta gagcggattg
    78541 gtgcaattat tattgatgag gagcatgaat caacctataa acaagaaagc aaccctagat
    78601 accatgccag agaggttgcc ctgctcagag ctaaacacca tcaagcagtt gtggttatgg
    78661 gctctgctac gccaagtatt gaaagccgag caagggctag taagggggtc tatcatttta
    78721 ttcaattaac gcaaagagca aatcctcttg ctaagattcc agaagtaacg attgttgatt
    78781 ttcgtgatta tattggacag caagcagtta gcaattttac cccatatttg attgataaga
    78841 ttaaagaacg tttggttaaa aaagaacaag ttgtcttaat gttaaataga cgtggttatt
    78901 ctagttttgt gatgtgtcgg gactgtggtt atgtggacaa atgtcccaac tgtgatatct
    78961 ccttaaccct tcacatggat actaaaacta tgaattgcca ttactgtggg tttcaaaaac
    79021 ctatcccaat tacttgtcct gagtgtcata gcaacagtat ccgctattat ggcactggaa
    79081 ctcaaaaagc ttttgatgaa ttgcaagggg ttatcccaga agctaggatc ttgagaatgg
    79141 atgttgacac cacacgaaaa aaaagaagtc acaaaaccat actagatagt tttggtcgcc
    79201 aggaagcaga tattttgtta gggacgcaaa tgattgccaa aggactggat tttccaaatg
    79261 tgactttagt tggggtacta aatgcagata cctccttaaa tttgccagat tttcgagcgt
    79321 cagaaaaaac gtttcaattg ttaacacagg ttgctggtcg ggcaggccgt gcccataagc
    79381 caggagaagt cttgattcaa acttataatc ccgatcacta tgctattcaa ttagctaaga
    79441 aacaagattt tgaagccttt taccgttatg aaatggcgat tcgccgtcag atggcttatc
    79501 ccccttacta ctttacagtg ggtattacgc tttcgcatcg tttagaagca tcggttgtaa
    79561 aaaaagctta tcaagttaca gaactgctaa agagtcactt atcggataat attaaaatat
    79621 taggaccgac tcctaagcca attgctagaa cacacaattt ataccactat caaattttgt
    79681 taaaataccg ttttgaagat aacctagaag agacgctcaa tcgtattctt gattggagtc
    79741 aagaagcaga taatcgtcat ttaaaactaa ttattgattg tgaaccgcaa caatttttat
    79801 aaggtaaatc actaaatcta ggatgatcag ttatgatata ataaaaatac aaagaggaaa
    79861 gtcatgataa aattattatt tatgggaact ccccaatttt cagcaactgt attgaagggc
    79921 ttgctagata atcctgccta tgagatttta ggagtggtta ctcagcctga tcgagctgtt
    79981 ggacgcaaaa aagatattaa ggtaacacct gtaaagcaat tagctcttga gcatggaata
    80041 tcaatctatc agccggaaaa attatcaggc tctcaagaac tcattgagat tatgggactt
    80101 ggtgcagatg gtattattac agcagcattt ggccaatttt taccaacact attacttgat
    80161 tcagtttcat ttgctattaa tgtacatgca tcattgttac ctaaatatcg aggtggagca
    80221 cctatccatt atgctattat gaatggagat aaagaggctg gcgttactat tatggaaatg
    80281 attaaagaaa tggatgcagg tgacatggtc gctaaagcaa gcactccaat tcttgaaacc
    80341 gataatgtag gtactctttt tgagaaatta gccatcattg gtcgagactt acttttggat
    80401 agtttgccag cttatctttc tggagaatta aaacctattc ctcaagatca cagccaagct
    80461 actttttcac ctaatatctc tccagaacaa gaaaaattag attggacaat gtctaatcaa
    80521 gaagtgttca atcatattcg tggtatgaac ccttggccgg tagcgcatac gtttttagag
    80581 gggcaacgtt taaaaattta cgaagctcaa ctagctgaag gagaaggatt acctggacaa
    80641 gttattgtca aaaccaaaaa atctttggta atagctactg gacaaggagc tttgtccttg
    80701 atcgttgtgc aaccggctgg taaacctaaa atgtcaataa tagacttttt aaacggcatt
    80761 ggtagaaaac ttgaagtagg ggacatcatt ggcagataat tggaaaaaaa gtacgcgagg
    80821 caaagccctc cttgtcattg aagctatttt tgatcaagga gcttacacaa acatagcgct
    80881 taatcagcag ctaagcaata aagctttatc tgctaaggac agagctctat taactgagat
    80941 tgtatatggt accgtttcac gtaaaattag cttggaatgg tatttagccc attatgtcaa
    81001 agaccgagat aaattggaca aatgggttta ttatctcctt atgctcagtc tttatcaact
    81061 gacttattta gataagttac cagcgcatgc cattgttaac gatgcggttg gtattgccaa
    81121 aaatcgtggc aataaaaaag gtgcagagaa gtttgtcaat gctattttac gtcaattcac
    81181 cagccatcct ctcccagata tggaaacaat taagcgtcga aataagtatt attcggtcaa
    81241 gtattcactg cctgtttggt tggttaaaaa gttagaagac caatttggct cccacagagc
    81301 agtggctatt atggagagtt tatttgtgcg tagtaaggct agtattcgcg tgacggaccc
    81361 tttgaagtta gaagaagttg ctgaggcttt agatgcagaa cgttcccttt tatcagcaac
    81421 tggtttaacc aaagcgtcag gtcattttgc agccagtgat tatttcacaa atggggacat
    81481 cactattcaa gatgaaagca gtcagcttgt agctcctact ttaaacattg acggtgacga
    81541 cataattctt gatgcgtgta gcgctccagg gggtaagacc tctcatatag catcttactt
    81601 aaaaacaggt aaagttattg ccttagatct ctatgatcat aaactagagt tggtgaagga
    81661 aaatgctaac cgcttaggag ttgctgataa cattgagaca agaaaactag atgccagaga
    81721 ggttcatcgt cattttgaaa aggatagctt tgataagatt ttggtggatg ctccttgttc
    81781 aggaattggt ttgattcgtc gaaaacccga tatcaaatat aataaagaaa gtcaagattt
    81841 taatgctcta caggctatcc agctagagat attgtctagt gtttgtcaaa ccttgcgtaa
    81901 aggtggtata ataacataca gtacttgcac tatttttgat gaggagaatc gtcaagtcat
    81961 cgaagcattt ttacagagtc atcctaattt tgaacaagta aaactgaacc atacacaagc
    82021 agatattgtc aaggatggtt atcttattat cacaccagaa caataccaga ctgatggctt
    82081 ctttattgga caagtcagac gagtcttgta gtattttagg aggaaactga ctgagtcaga
    82141 aaaaacgtat gaagatttca ttaaaaacag acattgggca aaaacgctca aacaatcaag
    82201 attttatcaa taagtttgat aataaaaaag gcatcacttt agtgatttta gcagacggta
    82261 tgggtggcca cagagctgga aatattgcca gtgagatgac agtcacagac ctgggaagag
    82321 aatgggtaaa aacagacttc acagagttaa gtcagattag agattggtta tttgaaacta
    82381 tccagtcaga aaatcaacgg atttatgatc taggccaatc agaagacttt aaaggaatgg
    82441 gaacgactgt tgaagcagta gctttagttg aatctagcgc catttatgct catattggtg
    82501 attcacggat tggtcttgtc catgatgggc attatacctt attgaccagt gatcattctt
    82561 tagtgaatga gttggttaag gcaggacaga tcacagaaga agaagctgct agccatccac
    82621 aaagaaatat tatcactcaa tctattggtc aagcttcccc agtagagcct gatttaggag
    82681 tgagagtttt ggagccaggg gattatcttg tcattaacag tgatggtcta accaatatga
    82741 tttcaaatga tgaaattgtg actatcttgg gcagtaaggt tagtcttgat gaaaaaaacc
    82801 aagaaatgat tgacttagct aacctaagag gaggcctaga taatattaca atcgctttgg
    82861 ttcacaacga aagtgaggac gtcgaatgat tcagattggc aaattatttg ctggtcgtta
    82921 tcgcattctg aaatctattg gccgcggtgg tatggcggat gtttatttag caaatgattt
    82981 gatcttggat aatgaagacg ttgcaatcaa ggtcttgcgt accaattatc aaacagatca
    83041 ggtagcagtt gcgcgtttcc aacgagaagc gcgggccatg gctgaattga accatcccaa
    83101 tattgttgcc atccgggata taggtgaaga agacggacag caatttttag taatggaata
    83161 tgtggatggt gctgacctaa agagatacat tcaaaatcat gctccattat ctaataatga
    83221 agtggttaga attatggaag aagtcctttc tgctatgact ttagcccacc aaaaaggaat
    83281 tgtacacaga gatttaaaac ctcaaaatat cctactaact aaggagggtg ttgtcaaagt
    83341 aactgatttc ggcatcgcag tagcctttgc agaaacaagc ttgacacaaa ctaattcgat
    83401 gttaggcagt gttcattact tgtctccaga acaggctcgc ggctccaaag cgacgattca
    83461 aagtgatatt tatgcgatgg ggattatgct ctttgagatg ttgacaggcc atatccctta
    83521 tgatggcgat agtgctgtta cgattgcctt gcaacatttt caaaagcctc ttccatctat
    83581 tatcgaggag aaccacaatg tgccacaagc tttggagaat gttgttattc gagcaacagc
    83641 caagaaatta agcgatcgtt acgggtcaac ctttgaaatg agtcgtgact taatgacggc
    83701 gcttagttat aatcgtagtc gggagcgtaa gattatcttt gaggatgttg aaagtaccaa
    83761 acccctcccc aaagtggcct caggtcccac cgcttctgta aaattgtctc cccctacccc
    83821 aacagtgtta acacaggaaa gtcgattaga tcaaactaat caaacagatg ctttacagcc
    83881 ccccaccaaa aagaaaaaaa gtggtcgttt tttaggtact ttattcaaaa ttcttttttc
    83941 tttctttatt gtaggtgtag cactctttac ttatcttata ctaactaaac caacttctgt
    84001 gaaagttcct aatgtagcag gcactagtct taaagttgcc aaacaagaac tgtatgatgt
    84061 tgggctaaaa gtgggtaaaa tcaggcaaat tgagagtgat acggttgctg agggaaatgt
    84121 agttagaaca gatcctaaag caggaacagc taagaggcaa ggctcaagca ttacgcttta
    84181 tgtgtcaatt ggaaacaaag gttttgacat ggaaaactac aaaggactag attatcaaga
    84241 agctatgaat agtttgatag aaacttatgg tgttccaaaa tcaaaaatca aaattgagcg
    84301 cattgtaact aatgaatatc ctgaaaatac agtcatcagt caatcgccaa gtgcgggtga
    84361 taaatttaat ccaaacggaa agtctaaaat tacgctcagt gttgctgtta gtgatacgat
    84421 cactatgcct atggtaacag aatatagtta tgcagatgca gtcaatacct taacagcttt
    84481 aggtatagat gcatctagaa taaaagctta tgtgccaagc tctagctcag caacgggctt
    84541 tgtgccaatt cattctccta gttctaaagc tattgtcagt ggtcaatctc cttactatgg
    84601 aacgtctttg agtctgtctg ataaaggaga gattagtctt tacctttatc cagaagtaac
    84661 acactcttct agtagctcat cgagttcaac gtcaagttca aacagttctt caacaaatga
    84721 tagtactgca ccaggtagca acactgaatt aagcccatca gaaactactg ctcaaacacc
    84781 ttaacaaaga atagttagtc tcatgtttta ctagcatgcc actaactatt ctttttaact
    84841 actcgtcata taaagatgca attttccagg aattctgata aaatacaaag aaataagggg
    84901 aaactaatga aaaaatttca attcttttta cttatcgagt gtatactgct agctatgggt
    84961 atcatgacaa tcttggataa tgatttatca agttttatcc taattctagt tcttatctta
    85021 ttagcattac gcttttataa ccaagatagc cgcaataatt ttttactaac tgtcagttta
    85081 ttgtttcttt ttttaatttt catgcttaat ccctacatta tcatggctgt tcttttgggg
    85141 atagtttaca ttttcattaa ccatttttca caagtcaaaa agaagaatcg ctttgccttg
    85201 attcgtttca aggaagaaaa gattgaagtg aacaatacca agcatcaatg gattggtact
    85261 gctaattatg aaagtgatta ttattgtttt gatgacatta acatcattcg aatctcagga
    85321 aatgacacgg ttgacttaac caatgttatt gtgacaggaa tggataatat cattgtgata
    85381 cgtaaaattt ttggaaatac gactatattg gtacctattg atatcactgt tactttagat
    85441 gttagttcca tatacggaag cgttgatttt tttagatgtc agcaatatga tttgcgcaac
    85501 gaatctatta agttaaaaga aacggataac caatccctta aaaaagtcaa aattatcgtt
    85561 acaactattg ccggaaatgt tgaggtggat aggcgatgaa aaaacgttac tatgctcttg
    85621 tttggctcta ctcaaccatt accattttat ctattgtttt tgtggtcatg gataatttag
    85681 gaatcacttt taactacctt cgcaatcatt tatggcaggt cgaacgtcta gggttttcga
    85741 ttttattgtt gattgtttct gtgactttat tattgttgtt attgtggatt attatggatg
    85801 ataacagtaa gcgtaacatc aaccaaaatc taaaatatat tctcaataat cgacgactct
    85861 atctagatga gacatcagaa atcaatacta atttaagtcg actttctaaa aaaatgtctc
    85921 acctgactgc taacatgcaa aagaaagaaa gtgcttatat tcttgatagc caagaagttg
    85981 taaaacaaga gcgcaaacga attgcaagag atttacatga cacagtcagt caagagctat
    86041 tcgcttcgtc attgatttta tcaggaattt cgatgagttt ggaacaactg gacaaaacac
    86101 aattacaaac acagttaaca acggttgaag caatgttgca aaatgctcaa aatgatctac
    86161 gcattctcct tttgcatctt agacctaccg agctagctaa tcggacttta tctgagggcc
    86221 ttaatatgat tcttaaggaa ttaacagata aaagtgatat tgaagtcatt tataaggaaa
    86281 ccattgctca gcttcctaaa acaatggaag ataatctttt tagaattgcc caagaattca
    86341 ttagcaacac gttaaaacat gctaaagcta gtcgaattga agtttatctc aatcaaacct
    86401 caacagaatt acaattgaag atgattgatg atggtgtagg atttgatatg gatcaggtaa
    86461 gggatttgag ttatggtctg aagaatattg aagaccgtgt caatgattta gcaggaaacc
    86521 tacatttaat tagtcaaaaa ggcaaaggag tttccatgga tattagactg ccgatagtga
    86581 agggagatga cgatgagtaa gataaaagtg atattggtcg atgatcatga aatggtccgc
    86641 atgggactca agagtttttt gaatttacaa gctgatattg atgtcgttgg tgaagcctct
    86701 aatggacgtg aaggggttga tttggcattg gccttgaagc cagatgtttt ggttatggat
    86761 ctagtgatgc cagagttagg cggtgttgag gcaactttag aagtcctaaa aaaatggaaa
    86821 gaggctaagg tacttgtgtt aacttcctat ctagataatg aaaagatata ccctgtcatt
    86881 gatgctggtg ctaaaggtta catgttaaag acttctagtg cagccgaaat tctaaatgct
    86941 attcggaaag tttcaaaagg tgaattggcc attgaaacag aggtggacaa aaaaattaaa
    87001 gcgcatgacc aacaccctga tttgcatgaa gagctaaccg cgcgcgagta tgatatttta
    87061 catcttttag ctaaagggta tgataatcaa actatagcgg atgaactttt catctccttg
    87121 aaaaccgtta aaacgcatgt atctaatatt cttgctaagc tagaagttga tgaccgaact
    87181 caagcggttg tctatgcttt tcgacaccat ttagtccccc aagacgataa ttaatgctat
    87241 aatatagggt atagcaaatt ggaggaaagt atggacgcaa aactaaaata caaagctaaa
    87301 aaaatcaaaa tggtgttctt tgatattgat gataccttga gggtcaaaga tacgggttat
    87361 atgcctgagt cgattcaaag agtatttaag gctttgaagg caaaaggcat cttggttggt
    87421 attgcttcag gtcgcgctcg gtacggtgtt ccacaagaag ttcaagattt acatgctgac
    87481 tattgtgtca aattaaatgg agcttatgtc aaagacgatg ctaaaactat tattttccaa
    87541 gctccgattc cagcagatgt tgttgtggcc tataaaaaat gggcggatga tatgggcatt
    87601 ttttatggaa tggcgggtcg tcatgaagcg gtgttgtccg ctcgaaatga catgattagc
    87661 aatgctattg ataatgtcta cgctcaacta gaggtctgtc cggattataa tgagtatcat
    87721 gatgtttacc aaatgtggac cttcgaggac aagggagatg gcttgcagtt gcctgctgaa
    87781 ttagcagagc accttcgttt ggtcagatgg cacgataact catcagatgt tgtcttgaaa
    87841 ggtacgtcaa aagcgctagg tgtttcaaaa gtagttgacc atttaggatt aaaacctgaa
    87901 aacattttgg tgtttggtga tgaactaaac gacttagagt tatttgacta tgctggtatc
    87961 agtattgcaa tgggagtatc acacccacgt ttgcaagaaa aagcagatgt tatcacaaaa
    88021 aaagttgaag aagatggcat tttatatgcc ttggaggaat taggattgat tgacaaagaa
    88081 ttacagtttc cacaattgga tttagaaaat catactggac caaaagtaac cattaaaaca
    88141 aatcatgggg atatgacttt ggtcttgttc ccagagcatt cccctaagac ggttgctaac
    88201 ttcttaggat tagctaaaga gggatactat gatggcatta ttttccaccg cgttattcct
    88261 gaatttatga ttcaaggtgg tgatccaaca ggaacaggaa tgggtggaca atccatctat
    88321 ggtgaaagtt ttgaagatga attttcggat gaactgtata accttagagg agccctttca
    88381 atggcaaacg caggcccaaa tactaatggt agccaattct tcattgttca aaatagcaaa
    88441 attccttacg ctaaaaaaga attagaacgt ggtggttggc ctactccaat tgcagctgct
    88501 tatgctgaaa atggtggaac tcctcacctt gatcggcgtc atactgtttt tggccaatta
    88561 gctgatgacg cttctttcaa agttttggac ctgattgcta gcgttgaaac tggcgcacag
    88621 gataaaccta aagaagacgt tatcattgaa acaattgagg tatttgacta atgaaaattg
    88681 gcgacaaact gcatggcacc atcactggga ttaaaccata cggagccttt gttgcccttg
    88741 acaatgggac aacaggtctc attcatattt cagaaataaa aacgggattt attgatgata
    88801 ttgatcaatt attggctata ggcaaccagg ttttagtaca agtaattgat attgatgaat
    88861 atagtaaaaa ggcaagttta tctatgcgga ccttagcaga agaaaaacag cattttttcc
    88921 atcgtcatcg ttattccaac agtcggcaca agattggctt taggccactt gaagagcagt
    88981 tacctcagtg gattgaagaa agcctgcagt ttttaaaaaa gaacactaaa aagagtctgg
    89041 aaccttagta gttccaaacc cttttttatt tcgtcttaat tactggtaca tcatagagtt
    89101 cagtcgataa atagcgttcg ccattatctg gtaaaatagt taacacatgt ttgccttttc
    89161 ctaactgttt agcgacttca atagcggcgt aaagagcagc tccagaagaa atcccaacca
    89221 ggaagccttc cttagctcct gttagtcgag cagtttctaa agcatctttc gatttaacac
    89281 ggataatttg gtcataggct ttggtatcta acgtgtttgg gataaatcca gctgatatac
    89341 cttgaatttt atgtggtcca ggctcttgac cagataagac agcagattcc tcagcttcaa
    89401 cagcatagat agcagtttca gggttagctt ttttcaagac atgtgaaaca ccagaaagtg
    89461 ttcctccagt accaacacca gatacgaatg catctaaaga aatctcctta aaagcttcca
    89521 aaatttcttg agctgttgtt ttttcatgga tgcttggatt ggcagggtta ttaaattgca
    89581 taggcatcca agcacctagt tctattgcta aagtttcagc ttttgcaata gcccctttca
    89641 taccttctgc tccaggtgtt aagacaagct ctgcaccata agcctgaatg atttgtcgtc
    89701 tttccaagct catagtttcg ggcataacaa taatgactcg atacccttta gcagcaccta
    89761 cccatgcaag accaatacct gtattaccgc ttgttggttc gataataacg tcaccaggac
    89821 ttatcagacc ttcagcttca gcagcttcaa tcatcgataa agcaatacga tctttaacag
    89881 aagatcctgg gttaaaagct tctaatttta cataaacgtc agcagcttcg tttggaatta
    89941 aacagttaag tttgataata ggtgtttgac ctactaattc tgttatagtt ttgtaaattt
    90001 tagtcatagt ttagcctctt ttcttgatac aaaataaata cgctgtgtat ttaagtataa
    90061 ggagagatat tattaaagta aaatacagat ttactatcac tctgataata aagccatatc
    90121 acttttgttg atgattaatc acaggggaat tcaataactt tggatccgat tttctcacag
    90181 cttatctgac catgataata atttgttaaa gtatcaatga gattttccac acgttcagta
    90241 tcacaataaa gtgttgtctt tatcgtgtct gaaaacttag tttctgtttc ggttaaagcc
    90301 aattgatcta gcaagttaga gtatagttgg tattggggat aagaaagtgt gatttctagt
    90361 cctatctgct gtttaacttc aatgatgcca aatcgtttaa tagctgttgc ggtcatgtta
    90421 gagtatgctc tgattaaccc acctgttcct aatttaatac caccaaagta acgcgttacc
    90481 acaactacta catttgttaa acactgtttt tctaacacag aaaggatagg tattccagca
    90541 gttcctgaag gttcaccatc atcacttgag cgcttgatct gactgttgtt accgataatc
    90601 atcgcaaagc aggaatgatt agctttgtaa tgttcttttt tgatagcatt aacaaaattc
    90661 ttaccatctt cttcagtgct cactcgtttg atatgacaaa tgaaacgaga ttttttaata
    90721 ctttcttcga aaaaaccact ggctttgatt gtcttaaaat gttccataaa aaaattgtac
    90781 aagaaaagat aaaaattagc aaaaaaagcg aataataaag tatggagtct attgaaaata
    90841 gttatgggcg gttgttgcta gaaaaccagt taccagattc agcaaggcag cttgctcaac
    90901 ctttaaaaag tgtagtaata tcgagaggaa aaatgatttg tcaacgttgt cactatcagc
    90961 ttgatgaaga agcacgattg ccaagtggag cctattattg ccgattttgt cttgtctttg
    91021 gacgaaatca atcaaataaa ttgctctatg ctatcccacc tatgcatttt ccaaaaggta
    91081 attatctggt ttggagcggt caactaactg cttatcaaga gatgatttcc caacaattat
    91141 taatcaatat gcaaaatcaa aaaacaacat tagttcatgc tgtaactggt gcaggaaaaa
    91201 cagaaatgat ttatgctgcg attgaggcgg taattaacac aggtggctgg gtttgcattg
    91261 ccagcccaag agtagatgtt tgtgtagagg tggctactcg tttatcccaa gccttctcct
    91321 gcagcatctg tttaatgcac gcagagtctc ttccttatca aagagctcct attatagtag
    91381 ccacaacgca tcagctgcta aaatttcaca aggcttttga tctcttaatt attgatgaag
    91441 tagacgcatt cccttttgta aataatattc aacttcatta tgctgcaagt caggctttaa
    91501 aagaaggtgg cgcaaagatt ttattaactg caacttcaac tagaacttta gaaagaaagg
    91561 taaacaaggg agaagtagtc aagctgacat tggcaagacg ctttcataat aggccattag
    91621 ttattccgaa atttataaga agttttaatc tatttaaaat gatacatcgg caaaaacttc
    91681 ctcctaaaat tttaaaatac ttaaaaaaac aaagaaaaac aggctatccc cttttaattt
    91741 ttctacccac tattataatg gcagaatctg ttacagcaat cttaaaagag ttattgcccg
    91801 ccgaacaaat agcttgtgtt tctagtcaat cacaaaacag gaaagaagat atcacagctt
    91861 ttcgtcaagg gaaaaaaacg attttaatta caacaacaat tttagaaagg ggtgtgacat
    91921 ttccccaaat tgatgttttt gttcttgggt ctcatcatcg tgtctatagt tcccaaagtc
    91981 ttgttcaaat tgctggacga gttggaagat ctatagacag accagacgga acgttatatt
    92041 tctttcatga aggtatcagt aaagcaatgc tactagcaag aaaagagatt aaagaaatga
    92101 attataaagg gtattcgcat gatctgtcta ctatgtcaac aaattagtca aacaccaata
    92161 agtattacag aaatcatctt tttaaggcgt atctcttcac cgatttgtca acaatgtcaa
    92221 aaaagctttc aaaagatagg aaaaagtgtt tgtgcgacat gttgtgcaaa ctcagatata
    92281 atagcttgtc gagattgtct aaaatgggaa aacaaaggat acaatgtaaa tcatagaagc
    92341 ttatattgtt ataatgctgc tatgaaagca tacttcagtc aatataagtt tcaaggagac
    92401 tatttattaa gaaaagtttt tgcagtagaa cttgccgatg ttattaccaa gtactataaa
    92461 ggctatatcc cagtcccggt tcctgtaagt cccggttgtt ttcgagaaag acaatttaat
    92521 caagtgagcg ctattcttga ggcagctaat gttagctacc tttctctttt tgaaaagcta
    92581 gataatactc accaatcttc cagaacaaaa aaagagagat tattagtaga aaaatcttat
    92641 cgactactaa aagtatcaaa cattcctgat aaaatcctta tagtagatga tatttatact
    92701 actggtagta caattatcgc tcttagaaaa caattggcta aagtagcaaa tagtgacatt
    92761 aaaagtttgt caattgcacg ttaaaacttg caaattttca tgaaagcagt ataatagtat
    92821 taaagataaa gtaagcgctt caagcgcgag aaagaggtat cctatgatta agttcagtat
    92881 tcgtggagaa aatatcgaag tgacagaagc tattcgcgat tatgtagaat caaagcttac
    92941 taaaatcgaa aaatattttg ctaaagacca agaaatagat gcacgtgtta atttaaaagt
    93001 ttatcgggag agatcttcaa aagttgaggt aacgatccca ttagattcag taacccttcg
    93061 tgctgaagat gtttcacagg atatgtacgg ctctattgat ttagttgttg ataagatcga
    93121 acgccaaatt agaaaaaata aaaccaaaat tgctaaaaaa catcgcgaaa aggttccaac
    93181 tggtcaagtc tttacaactg agtttgaagc tgaagaagtc gatgagatac cagaagtaca
    93241 agttgtacgt actaaaaatg tgacattaaa accaatggat gttgaagaag cgcgtttaca
    93301 gatggaacta ttgggacatg atttctttat ctacactgat tctgaagatg gtgcaacaaa
    93361 cattttatac cgtcgcgaag atggaaacct aggattaata gaagctaaat agttcacaac
    93421 taaaacctac agggatgatt atacgtattt taaaagtggg aaaaagctat tcatgtttcg
    93481 aggtgacatg atgaaaatga gattaagtga tagatacgac agtcattata taacaaaagt
    93541 atctattaca gttcctcttt aagtaaatct tacgtaaaat tagtttgaaa aaaagattag
    93601 tcccaattct gggctagaaa ttctcaaaaa atatgaaaaa gcgcgttttc agaatataaa
    93661 gcgcgttttt ttctttttcc atttaacaat aaaaaactca tgacgaacat ggtatcataa
    93721 gagaattttt actttttaaa aaaaggtcaa agactttctg acaccaaata aaaatagaat
    93781 tttttctaac taaaaataat agcacaatct ttggcagcaa tctgaattgt ttttccataa
    93841 aagatttaag cacttacgtt tgcttaaaat ataggttatg tgacgtgcgt gcaagatcag
    93901 attataacaa aatagttcca aataagccta tcactgagaa agcatcacag aagggttaaa
    93961 ctttttttgt attagtgttt tatcagtaca acctaacact tatttttata aaaaaataaa
    94021 aaagctattg acaatcttat acaagataac gtatactaat aaagctgttg taaatatggt
    94081 aaagcaatag atgctatcag gaaaaaatgt gaaaacactt attgaaaaaa gatgaaaaag
    94141 tagttgacag gctatgagaa acctgataga ataaagaagt tgtctcttag gagacgttaa
    94201 gacctttgag aactgaataa gacgaaccaa acgtgagggt gatatggaga catattaccc
    94261 gtcaagaaac gagaaataaa tctgtcagcg aca
//
LOCUS       DAUGHY010000002        82584 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.002, whole genome shotgun sequence.
ACCESSION   DAUGHY010000002 DAUGHY010000000
VERSION     DAUGHY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 82584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82584
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.002"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            176..1735
                     /locus_tag="VH752_000092"
     CDS             176..1735
                     /locus_tag="VH752_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEQ8288478.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIGMMLTKGQLPYSDTFTTGGLFYFVIIALSYY
                     LGSTLWLVFVQVFCFYLSGLYLYKLINYMTGFQKVALTFSISYYLLSVSLGFGGLYPT
                     QLAMPFILISAWFLTKYFACLVKDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSY
                     NISQKHLARGFYQLLASIFGMILVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNL
                     SLLFGLAIQLFFALGLGLLTGMENVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDY
                     RPYHLLPLLPFGLILTAIPVGYQYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPI
                     LIVGTILICSTYCFISSIPLNQERDHIASYLEQKLNKTQSIYVWDDTSKIYLDSKAKS
                     VSQFSSPDINTQKVSHRKILEDELLENKAAYIVVNRYKNLPKIIQKVLSTNYKVDKQI
                     TTKSFIVYQKK"
     gene            1832..4030
                     /gene="nrdD"
                     /locus_tag="VH752_000093"
     CDS             1832..4030
                     /gene="nrdD"
                     /locus_tag="VH752_000093"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEQ8288479.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYTIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIDLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIVKAMKQACENWSDEYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            4212..4358
                     /locus_tag="VH752_000094"
     CDS             4212..4358
                     /locus_tag="VH752_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288480.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRVQELKARFLEKSK
                     KQDN"
     gene            4387..5322
                     /locus_tag="VH752_000095"
     CDS             4387..5322
                     /locus_tag="VH752_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ8288481.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGHAILYSDVKELSKTNLDGVFIHAATKAHVDLASLFLRQGIPVFMDKPLADNYDSV
                     KYLYELAEAHHTFLMAGFNRRFAPCVKELSSLSTKRKVAAEKNDLNRPGDMTFKLFDF
                     FIHPLDTALFLTEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLGFASWDTTLHKRGFETMIDAFLEAISTGV
                     NPVSPESSLLSHWICQQIADSQLSYGELTVELPKD"
     gene            5331..5840
                     /locus_tag="VH752_000096"
     CDS             5331..5840
                     /locus_tag="VH752_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8288482.1"
                     /translation="MEIRRPTLKDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLASQGVPAIQYVAFDEKNQALGFLNLRLRLNASLLEKGGHIGYSVRPS
                     QRGKGYAKEMLKQAVSCAISKNITTILVTCDETNVASRAVIVANGGVLEDSRGGTERY
                     WIEEQRGDK"
     gene            5840..6454
                     /gene="nrdG"
                     /locus_tag="VH752_000097"
     CDS             5840..6454
                     /gene="nrdG"
                     /locus_tag="VH752_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEQ8288483.1"
                     /translation="MAEKCWNNPKPKEWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRCELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLF"
     gene            complement(6628..7359)
                     /gene="yaaA"
                     /locus_tag="VH752_000098"
     CDS             complement(6628..7359)
                     /gene="yaaA"
                     /locus_tag="VH752_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEQ8288484.1"
                     /translation="MLTFLIPTAKEMVIPKESYPHLLPQPSQAILKAMAAMTTEDLAK
                     SYRIKEEAAKKEQQRWQDMASQQSLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWISPKFMAEKEGRFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFYYHPDLSTDHEFVYIKKEA"
     gene            complement(7494..8249)
                     /locus_tag="VH752_000099"
     CDS             complement(7494..8249)
                     /locus_tag="VH752_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ8288485.1"
                     /translation="MTYPYNSTISLGKVSLNVTDLAKMTTFYTSIIGLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPANKAYGLYHTAFLVPDRHSLGLHFLTRGISLEGAADHGY
                     SEAIYLSDPEGNGIEIYHDKAVEQWDIRDNGQIIGVTEPTDTKSILEQLTDIPKHFLL
                     AQDTRISHVHLSVKNALDSSLLYQKVFDLGDKMTIPSASWIASGNYHHHLAFNHWSAP
                     YLKKHQEGTPGLAFLTIHIETPLLFSATLKKAR"
     gene            8435..8773
                     /locus_tag="VH752_000100"
     CDS             8435..8773
                     /locus_tag="VH752_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8288486.1"
                     /translation="MLKKRPACPVETTLSVIGNKWKLLILRDLLKGTLRFGQLKSSIG
                     SVSQKVLTAQLRAMEADGLVHRELYAEVPPRVEYSLTETGLSLAPVIEAMSDWDQTYQ
                     EKHADLFEEG"
     gene            complement(9052..9765)
                     /gene="treR"
                     /locus_tag="VH752_000101"
     CDS             complement(9052..9765)
                     /gene="treR"
                     /locus_tag="VH752_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEQ8288487.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIKHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKFKFLDFSRRRPK"
     gene            9976..12000
                     /gene="treP"
                     /locus_tag="VH752_000102"
     CDS             9976..12000
                     /gene="treP"
                     /locus_tag="VH752_000102"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEQ8288488.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKAVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSGIEGVSKEAAKSAAKSNQNAL
                     QRVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYAVAGTPAAEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLEIFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWIIGKGISFVVLAGLTGPVKWLFGAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFDVQANSIG
                     VGGLPGFMAINVKYMIPFFICMAVAIVVPMFLTFFFRKSHIMTKTEDEAKLAETPVSE
                     APAATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGVLLQPTEGVLVAP
                     CDAEVSVLFPTKHAIGLVTAEGLELLMHIGMDTVNLDGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATEMPLVVTNQDTFTVTVEGSLPHQIKANDKLVVAVKK"
     gene            12067..13695
                     /gene="treC"
                     /locus_tag="VH752_000103"
     CDS             12067..13695
                     /gene="treC"
                     /locus_tag="VH752_000103"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEQ8288489.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLRGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTAVNPDFGTMADFEDLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMSQGNGWNALFYNNHDQPRALNRFVDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDNYVDVESLNAYSSLLVSGKSAE
                     EAFAIIKAKSRDNARTPMQWDASEHAGFTTGKPWLEVGKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQAVYAFERHLGDQCLLVLNHFYADEVELELPPR
                     YQHGQVLISNYEKVSICEKVILKPYQTLAILADN"
     gene            13903..15798
                     /gene="pepO"
                     /locus_tag="VH752_000104"
     CDS             13903..15798
                     /gene="pepO"
                     /locus_tag="VH752_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEQ8288490.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGENIPDDAILANFVKFHRLVADYAKRDEVGVSPILPLIEEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPDTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYAWA
                     DFTKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDEIRVQSGTYARALSGTPQAMDKQKAAFYLAQGPFNQALGLWYAGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYA
                     KKVIDETLSLVENAQNLAKITIAHTWSKWNKPVDRSEWHMPAHLVNAYYDPQQNQIVF
                     PAAILQAPFYSLDQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNDWWTQEDYA
                     AFKERTDKIVAQFDGLESHGAKVNGKLTVSENVADLGGVACALEAAQSEEDFSARDFF
                     INFATIWRMKAREEYMQMLASIDVHAPGELRTNITLTNFDAFHETFDIKEGDAMWRAP
                     KDRVIIW"
     gene            complement(15965..17005)
                     /locus_tag="VH752_000105"
     CDS             complement(15965..17005)
                     /locus_tag="VH752_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEQ8288491.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVIEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKTDEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(17139..17906)
                     /gene="rpsB"
                     /locus_tag="VH752_000106"
     CDS             complement(17139..17906)
                     /gene="rpsB"
                     /locus_tag="VH752_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEQ8288492.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADVAFEADTQADSIEEIVEVVEGDNA"
     gene            18210..21284
                     /locus_tag="VH752_000107"
     CDS             18210..21284
                     /locus_tag="VH752_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ8288493.1"
                     /translation="MTKGIRFQLLGSPHIFLDDKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNMQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLNKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPVATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALVILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGRLESICL
                     EPFNRQESLAYINSQLGSQPVTAEEMEHLYQASQGNPFFLSEYTQALLRHEKFVPLTP
                     AIKAKLGLKLANLSSRDDALLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYDRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMEE
                     QFDKIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKHIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTPGLQANYAIQIAAALDYLGEIAQIRYQFEKAVTY
                     QKQAIALTENKPAELSVSIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLEIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDKGLVFYLMAVVKYQLIFQGA
                     TLSQQEKEMADHLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(21315..22301)
                     /locus_tag="VH752_000108"
     CDS             complement(21315..22301)
                     /locus_tag="VH752_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEQ8288494.1"
                     /translation="MLEDYYPSTTSYYHGGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSPSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYQMGHQKVCRAIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGVDPN
                     LAVLVLQHIAASRKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHS"
     gene            complement(22487..24028)
                     /gene="hutH"
                     /locus_tag="VH752_000109"
     CDS             complement(22487..24028)
                     /gene="hutH"
                     /locus_tag="VH752_000109"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEQ8288495.1"
                     /translation="MTRVINLDGESLTIEDVIAIARQGVACRIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKGVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAIGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLLEGSQNTTVATQSRV
                     QDPYTLRCIPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KSENHELGKGTKVAYDLFRKEVNFIEHDKHIEIYDELNKASTVIEDPSFLEAVEQTVE
                     LSIQF"
     gene            complement(24039..25391)
                     /locus_tag="VH752_000110"
     CDS             complement(24039..25391)
                     /locus_tag="VH752_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEQ8288496.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCVCTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRDPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNMGESIMVPRYGAAIADEGLLPAAIAKQNQNGAPLVAI
                     LVSGAIAIILLLTGSFENLAKLSVVFRFFQYIPTALAVMKLRKDDPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMDQMPPKEQI
                     "
     gene            complement(25611..26207)
                     /locus_tag="VH752_000111"
     CDS             complement(25611..26207)
                     /locus_tag="VH752_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEQ8288497.1"
                     /translation="MTTVMVKTPADYTQTDWSGGETNQLFLYPEDGDYKKREFSYRLS
                     KSTVALRQTTFTLLDGYHRILMTLDKPITLSDLSHAKEISLTPFEPYAFEGDTRIVSR
                     GICQDINLMYDDQYQGQLLPVWSDTLFETPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVIEQEASLSDLKVCPNQTSPAQTPVAVWAGLWYKT"
     gene            complement(26229..27902)
                     /locus_tag="VH752_000112"
     CDS             complement(26229..27902)
                     /locus_tag="VH752_000112"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ8288498.1"
                     /translation="MVLSDIEIANSVTMEPISKVADQLGIDKEALCLYGKYKAKIDAR
                     QLVALKNKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGVPREDGYDITVASEIMAILCLSENISDLKA
                     HLEKIIIGYNFQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVPKADLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACDKRGVDVVISDVWANGGAGGRELAEKVVALAEQDNQFRFVYNEDDSIETKLT
                     KIVTKVYGGKGIKLTPTAKRELAELERLGFGNYPICMAKTQYSFSDDAKKLGAPTDFI
                     VTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(27920..28546)
                     /locus_tag="VH752_000113"
     CDS             complement(27920..28546)
                     /locus_tag="VH752_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEQ8288499.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPAPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQDIIEDLHTKSSRLQQDLLDAIDKDTEAFNLVSDVFDMPKETDEDKA
                     ARRTAMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     GAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(28557..29456)
                     /gene="ftcD"
                     /locus_tag="VH752_000114"
     CDS             complement(28557..29456)
                     /gene="ftcD"
                     /locus_tag="VH752_000114"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEQ8288500.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATAKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTQ
                     ECVEISKQVAERINRELGIPIFLYEDSATRPERQNLAKVRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGITAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEIIGLAPAKALIDV
                     AEYYLQVEDFDYHKQILENHLLG"
     gene            complement(29544..31574)
                     /locus_tag="VH752_000115"
     CDS             complement(29544..31574)
                     /locus_tag="VH752_000115"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEQ8288501.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFKEGIRRSPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRWRPKDRIYGKPIDEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFKSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKSVAI
                     IAEVDQSRIETRHSQGWVSQIAESPEEALQLARKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNVYDGGYCPVGISFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGKHEDLVATDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTIALKFNELVREGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVTSAVDQLFH"
     gene            31865..33130
                     /locus_tag="VH752_000116"
     CDS             31865..33130
                     /locus_tag="VH752_000116"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEQ8288502.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDG
                     LIVALGSGEPDAELVGPQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGNPKAYLDVIIKDMLPVVKEENLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITNDDIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYPFYFFATNLIHQVYKKGQLTVDRGRIL"
     gene            complement(33188..34720)
                     /gene="ahpF"
                     /locus_tag="VH752_000117"
     CDS             complement(33188..34720)
                     /gene="ahpF"
                     /locus_tag="VH752_000117"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEQ8288503.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHDSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     ADTANMTIIKNVATKDIVGDDHVTGLNYTERDSGEDKHLDLEGVFVQIGLVPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(34741..35301)
                     /gene="ahpC"
                     /locus_tag="VH752_000118"
     CDS             complement(34741..35301)
                     /gene="ahpC"
                     /locus_tag="VH752_000118"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEQ8288504.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            35707..35777
                     /locus_tag="VH752_000119"
     tRNA            35707..35777
                     /locus_tag="VH752_000119"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35739..35741,aa:Cys,seq:gca)
     gene            36296..36499
                     /locus_tag="VH752_000120"
     CDS             36296..36499
                     /locus_tag="VH752_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEQ8288505.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            36695..37156
                     /locus_tag="VH752_000121"
     CDS             36695..37156
                     /locus_tag="VH752_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEQ8288506.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAMASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            37156..39600
                     /locus_tag="VH752_000122"
     CDS             37156..39600
                     /locus_tag="VH752_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ8288507.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHCLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKTPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDNGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKSAPETLTPID
                     QALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSGIPLEKLTQADSKKYL
                     NLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKAL
                     AEVLFDDEAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTQKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFG
                     VKGIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQDNMRDVVKIMVQPLIT
                     TLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIEDKLSELILSRELTSGH
                     TLKIGLSHGKLTFHIA"
     gene            39778..40068
                     /gene="groES"
                     /locus_tag="VH752_000123"
     CDS             39778..40068
                     /gene="groES"
                     /locus_tag="VH752_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEQ8288508.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            40104..41735
                     /gene="groL"
                     /locus_tag="VH752_000124"
     CDS             40104..41735
                     /gene="groL"
                     /locus_tag="VH752_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880885.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEQ8288509.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAPAMPAGMDPGMMGGF"
     gene            41986..42252
                     /locus_tag="VH752_000125"
     CDS             41986..42252
                     /locus_tag="VH752_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8288510.1"
                     /translation="MNHFGEILKTFRESKGLRLKDVAKAGISTSQLSRFEKGETDLTI
                     STFMLILDESNMPIDEFMYAVHDFHRDDLNELLSKSEAFRNNSR"
     gene            complement(42964..44463)
                     /locus_tag="VH752_000126"
     CDS             complement(42964..44463)
                     /locus_tag="VH752_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ8288511.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDLSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGVSMSATVSASANDAIQKIDPYVKNGLAESSMASVILPSVKTAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVAKKAKSYIEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGGVMWLSIGSPRNAPYLPYLGNISRTYEAY
                     QEKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQDKSTKEISD
                     LVVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVKEKPKNKKGLNRS"
     gene            44710..46350
                     /locus_tag="VH752_000127"
     CDS             44710..46350
                     /locus_tag="VH752_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288512.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLIGGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNQKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DKAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTIYVLDFTATYEFLYDNSTDPEQHTSGHINQDLTGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDQKGNRSKETRTAKISKVEDKGNGFYLYTPDPGSDISALAPEGGLGGANVKYA
                     YGFKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(46439..47440)
                     /locus_tag="VH752_000128"
     CDS             complement(46439..47440)
                     /locus_tag="VH752_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ8288513.1"
                     /translation="MTSVKVLKLSLLLLPPKWLSTLLKNTITGSMKTTNKPSKGFTVT
                     LKNPFEELTVKALLEEQLLIPRKIRHFLRTKKHVLINGHPVNWQSCVKYGDQVKLFFD
                     HEDYPEKIIVMGQAEKVTCLYEDEHIIIVNKPEGMKTHGNDPTELALLNHVSAYTGQT
                     CYVVHRLDKETSGAILFAKTPFILPILNRLLEKRDIHREYLALVHGSLDSPRVTYHHP
                     IGRHRHDRRKRVVDPINGKKAITEVTLVKNFHKTASLLNCQLQTGRTHQIRVHLAHQG
                     HVLFGDPLYSNGKKDCARLMLHAYQLRLKHPLTREDICVQAKSATFDAVLNAQKARK"
     gene            48016..48396
                     /locus_tag="VH752_000129"
     CDS             48016..48396
                     /locus_tag="VH752_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ8288514.1"
                     /translation="MKTIRRYDVNEDRGHTGLVEAGDFYYLNYCVGNVGQDIESQING
                     AFDEMERRLALVGLTLDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFA
                     HHGGPQGLLFQVDGVAYSKHISMT"
     gene            48435..50771
                     /locus_tag="VH752_000130"
     CDS             48435..50771
                     /locus_tag="VH752_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ8288515.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANH
                     LDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     GLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            50820..50972
                     /gene="rpmG"
                     /locus_tag="VH752_000131"
     CDS             50820..50972
                     /gene="rpmG"
                     /locus_tag="VH752_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ8288516.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            50986..51162
                     /gene="secE"
                     /locus_tag="VH752_000132"
     CDS             50986..51162
                     /gene="secE"
                     /locus_tag="VH752_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEQ8288517.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(51399..52172)
                     /locus_tag="VH752_000133"
     CDS             complement(51399..52172)
                     /locus_tag="VH752_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEQ8288518.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KVPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLIDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            52298..53044
                     /locus_tag="VH752_000134"
     CDS             52298..53044
                     /locus_tag="VH752_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ8288519.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLDVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQTKRNIKVITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSASGLVLDFVS"
     gene            53060..54040
                     /locus_tag="VH752_000135"
     CDS             53060..54040
                     /locus_tag="VH752_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEQ8288520.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTLISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRQQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFMNAMVIQEDKHLAKGILQSKYFNDILTSWDQLDLALVGIGGEPNSLE
                     QSQWRDLLTSSDHDQLKYEKAVGEVCCRFFDQAGQPVYTGLQDRTIGISLEQLRRVPK
                     TMAVATGKHKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            54327..54647
                     /locus_tag="VH752_000136"
     CDS             54327..54647
                     /locus_tag="VH752_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ8288521.1"
                     /translation="MQVIVPDQIIMGLILNAGDAKQHIYQALKCAKEDDYATSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFVHSQDHLMTTITEINLVKEIIDLRKE
                     LATK"
     gene            54675..54983
                     /locus_tag="VH752_000137"
     CDS             54675..54983
                     /locus_tag="VH752_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ8288522.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIEAYAQGKLAD
                     YAPLLDVALLGPQVAYTLDQSEAICKDNDIPIAVIPMADYGMLDGNKVLDLALSLVKE
                     "
     gene            54993..56297
                     /locus_tag="VH752_000138"
     CDS             54993..56297
                     /locus_tag="VH752_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ8288523.1"
                     /translation="MAKMNMQNIIMPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGINDAIAGVFGADWTEPFMQVYHGTFAIMGLISCFAIGYSYAKNSGVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEPVGDAISKAWFGGQGIIGAIVIGLTVGAIYTAFIR
                     RHIVIKMPEQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIISGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQASEES"
     gene            56508..58925
                     /locus_tag="VH752_000139"
     CDS             56508..58925
                     /locus_tag="VH752_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEQ8288524.1"
                     /translation="MTETKSPYFGHLTDRMTHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEASIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGQLTPEELWQALESDFAGERGEEIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            58938..59606
                     /locus_tag="VH752_000140"
     CDS             58938..59606
                     /locus_tag="VH752_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ8288525.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGDIDFFERI
                     REVRAIIGDKASIHVQVIAQDYKGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYSTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIGQLAEAIDRECS
                     DSKLLAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAIHH
                     RKSI"
     gene            59663..60751
                     /locus_tag="VH752_000141"
     CDS             59663..60751
                     /locus_tag="VH752_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEQ8288526.1"
                     /translation="MKVFASPSRYIQGKNALFTNAKTLKQLGNSPILLCDDVVYGIVG
                     ERFESYLIDNGMTPVHVAFNGEASDNEISRVVAIAKENGNDVIIGLGGGKTIDSAKAI
                     ADLLAVPVIIAPTIASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVICQAP
                     KRLLASGIADGLATWVEARAVMQKNGDTMAGGNQTLAGVAIAKACEQTLFADGLKAMA
                     SCDRQVVTPALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALTGAIHHLTHGEKVA
                     YGTLTQLFLENRSREEIDRYIDFYQAIGMPTTLKEMHLDTATQEDFLKIGRQATMAGE
                     TIHQMPFVISPEDVAAALVAVDAYVTSR"
     gene            complement(60833..61342)
                     /locus_tag="VH752_000142"
     CDS             complement(60833..61342)
                     /locus_tag="VH752_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEQ8288527.1"
                     /translation="MSTDHHLREVSYQLLAERGGSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGNSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            61706..62521
                     /gene="sdaB"
                     /locus_tag="VH752_000143"
     CDS             61706..62521
                     /gene="sdaB"
                     /locus_tag="VH752_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HEQ8288528.1"
                     /translation="MNLLGSRRVFSKKCRLVKFSMVALVSATMAVTTVTLENTALARQ
                     TQVSNDVVLNDGASKYLNEALAWTFNDSPNYYKTLGTSQITPALFPKAGDILYSKLDE
                     LGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWTGNPNHVKYKIEWLNGLSYVGDF
                     WNRSHLIADSLGGDALRVNAVTGTRTQNVGGRDQKGGMRYTEQRAQEWLEANRDGYLY
                     YEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYNTANGYTINYHNGTPTQK"
     gene            complement(62762..63604)
                     /gene="ropB"
                     /locus_tag="VH752_000144"
                     /gene_synonym="rgg1"
     CDS             complement(62762..63604)
                     /gene="ropB"
                     /locus_tag="VH752_000144"
                     /gene_synonym="rgg1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="RopB (Regulator of Protease B) is a paralog of the
                     quorum-sensing transcriptional regulators Rgg2 and Rgg3,
                     which bind short, hydrophobic peptides encoded by genes in
                     their respective vicinities. RopB binds the leaderless
                     short peptide SIP (SpeB-inducing peptide), with sequence
                     MWLLLLFL; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     RopB/Rgg1"
                     /protein_id="HEQ8288529.1"
                     /translation="MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSK
                     KLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFECRNIDGLNHILDSYKDSK
                     STKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFIFESC
                     FIEMVFSKVILNLDKYNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQL
                     NDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIK
                     HKLS"
     gene            64103..64273
                     /locus_tag="VH752_000145"
     CDS             64103..64273
                     /locus_tag="VH752_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288530.1"
                     /translation="MDNPKKDKGFSLGVVDPITILITESVINKMRTVPYHLKKLSSTT
                     YCLLADLAYQSA"
     gene            64545..65741
                     /gene="speB"
                     /locus_tag="VH752_000146"
     CDS             64545..65741
                     /gene="speB"
                     /locus_tag="VH752_000146"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HEQ8288531.1"
                     /translation="MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDANGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSAGSSRVQRALKENFGYNQSVHQINRSDFSKQDWEAQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            65743..66048
                     /locus_tag="VH752_000147"
     CDS             65743..66048
                     /locus_tag="VH752_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HEQ8288532.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     GGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMVEAYQKQINSLD"
     gene            66528..67457
                     /gene="prsA"
                     /locus_tag="VH752_000148"
     CDS             66528..67457
                     /gene="prsA"
                     /locus_tag="VH752_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ8288533.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVSD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTTEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTIAADKKITYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPATSKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAQK"
     gene            complement(67728..67964)
                     /locus_tag="VH752_000149"
     CDS             complement(67728..67964)
                     /locus_tag="VH752_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288534.1"
                     /translation="MTHRFFLTPLSTLWRRLRPKRYSKKVLADEPTSYQPPTAHGPCD
                     DTHQLLEDKSLSDDYLITTSAMRRLWYWGYSQIG"
     gene            68447..70627
                     /locus_tag="VH752_000150"
     CDS             68447..70627
                     /locus_tag="VH752_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEQ8288535.1"
                     /translation="MLIAPTVLGQEVSNTEASASSTASVDATASGTTASGASSEATVA
                     TTNGGSQSTQVAAETTPQPQVQTSEQPAATSTSLASSTSSSEDKAPKAASTKSSSATV
                     ASSSNGSNQGAGTEVEPQMMDVEQYKVNKEKTELTVKDDNQQLKIRKDVDELKNKDLF
                     DVKREVKDNGDGTLDVTLKVTPKQIDEGADVMALLDVSQKMTDADFNNAKEKIKKLVK
                     TLTSKPTDSHPNHNARNSVRLMTFYREVSDPIDISGKTDEELNKILDDVWQNAKHNWD
                     WGVDLQGAIHKARGIFKQDQEKKSGKRQHIVLFSQGESTFSYDIQKSEKERDNNLSRI
                     NEKITSSNPLLHWPPIFNHTHQNADMLNEIERIIKIGEQLGIKGLSSIKGYLSTAAAG
                     SGILGNFIGGGSLTEYLTLKEYNSDKLEESQFDYTKRVGEGYYYYSFSKRVSHNDFPF
                     KTLIEQAITQAIPTKGESKNWFEKFLDKFSLVESYRDTQKKALLKILEYLFYKREYHY
                     YNHNLSAIAEAKMAQQEGITFYSVDVTDSETTPKRVRRQTAVRQEKAKKESKEERNEK
                     FDKYLKKMSEGNTFLDSVEEKDKFKDTFTNVTVTETFEDKVSAIANSWESSLDSHNNK
                     SDRNHKGSVKHTQAQSSGWFANTQESLTWTISKDQLKKAFEDGEPLTLTYKLKVDKDK
                     FRETLKKQQERNRKNEQHLRKVRTLSQKKLFQIRFLTRLIMVRI"
     gene            70654..71445
                     /locus_tag="VH752_000151"
     CDS             70654..71445
                     /locus_tag="VH752_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ8288536.1"
                     /translation="MSYSKFKMPIPELDIEVVPIPEKPLVEPMTPLYPAIPNYPTPQL
                     PKDEDLEISGGHGPIVDIIEDTGTGVEGGSQNGVVSTQENKDPIVDITEDTQPGMSGS
                     NDATVVEEDTAPKRPDVLVGGQSDPIDITEDTQPSVSGSNDATVVEEDTAPKRPDILV
                     GGQSDPIDITEDTQPGMSGSNDATVIEEDTKPKRFFHFDNEPQAPEKPKEQPSNSLPQ
                     APVYKAAHHLPASGDKREASFTIVALTIIGAAGLLSKKRRDTEEN"
     gene            71625..73592
                     /locus_tag="VH752_000152"
     CDS             71625..73592
                     /locus_tag="VH752_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ8288537.1"
                     /translation="MTKKFIKSAVILGLASITISMTQPVEAISNSLEYQAQYKRVKEE
                     LDALKEKGPKLGERSLYFKVVNRFLGWFGVVWDPLAIKETDHLLDLIGQVNYLVSNTE
                     FQAPEYQKAKNKLDLAVAGYDLYLAREKKRGDAPDYQVTQLTLGQLETIKTKIQEALK
                     TFAQEVKTIQQKKPDLFPHAYVLNAKSKDITDKAQPFLVNGYYDVFYKQFLFTNTNDG
                     YNYNKEADNLASYQKLIKELLGLKAEIDQLTKDDPKNDRLETLMWESKFTLSDSRRTS
                     RLSAYYTQEDYVKKVVLSEEAPNLRTAKEKVKTLKGILHDYYVDIKEPEKAKKYRVED
                     ETLPSQPEAPAKPEAPAPSPSPAPGQKPAEDERSSQATEPANPSKENSSTDASQGSHD
                     SENPATDSPSQPQASDQGNHQSQVPNAKPQTDKTDTPNAPVPPQNTPNAPAIPQDTPK
                     VPEAGGQSGPAGNAEEKAPDSGPKESGQSSSKESPSVGEDTTPNQPDVLVGGQREPID
                     ITEDTITDAPPTVSGHNASTQPQSVVEDTAPQRPDVLVGGQSEPIDITQDTQPGMSGS
                     NDATVINEDTKPKRVFHFDNKEPQASEKAAEQKLAPHDSHTTPQTSDDTAAPHLPASG
                     DKPEKVFALAALTMVAAAGLLSKKRRNTDKN"
     gene            73734..74030
                     /locus_tag="VH752_000153"
     CDS             73734..74030
                     /locus_tag="VH752_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEQ8288538.1"
                     /translation="MTKQDQLIVEKMEQTYEAFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADILDLAPIMYKHM"
     gene            74178..74462
                     /locus_tag="VH752_000154"
     CDS             74178..74462
                     /locus_tag="VH752_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEQ8288539.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            74886..76154
                     /locus_tag="VH752_000155"
     CDS             74886..76154
                     /locus_tag="VH752_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ8288540.1"
                     /translation="MFQLRKKMTRKQLALLSAGVLTCVVGGTYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKDVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATATMEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKTYVWKVDKNKVKKQEVKTKPFSKGYIE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            76141..76815
                     /locus_tag="VH752_000156"
     CDS             76141..76815
                     /locus_tag="VH752_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8288541.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            76828..78045
                     /locus_tag="VH752_000157"
     CDS             76828..78045
                     /locus_tag="VH752_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8288542.1"
                     /translation="MEDIIAALGSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTINIVFNKKSSIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVK
                     NALISYGIDDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYL
                     EKSLYESLFPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNI
                     NGVVDATPEITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLN
                     KSNFVLLAGIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEA
                     VILTLLGGVIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIK
                     ASKLDPIEALRFE"
     gene            78139..78792
                     /locus_tag="VH752_000158"
     CDS             78139..78792
                     /locus_tag="VH752_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8288543.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            78789..80177
                     /locus_tag="VH752_000159"
     CDS             78789..80177
                     /locus_tag="VH752_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ8288544.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKR
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLI
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKPGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHHDD"
     gene            80279..81907
                     /locus_tag="VH752_000160"
     CDS             80279..81907
                     /locus_tag="VH752_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ8288545.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNNTSSTDQPTTAD
                     TDTDDESETAKKDKKSKETASQHDTQKDHKSSHTHPTPPSNDTKQTDQASSEATDKPN
                     KDKNVTKQPDSSDQSTPSPKDQSSQKESQNKDGRPTPSPDQQKDQTPDKTPEKSADKT
                     PEKGPEKATEKTPEPNRDAPKPIQPPLAAAAPVFAPWRESDKDLSKLKPSSRSSAAYV
                     RHWTGDSAYTHNLLSRRYGITAEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVR
                     AIVAIAMAESSLGTQGVAKEKGANMFGYGAFDFNPNNAKKYSDEVAIRHMVEDTIIAN
                     KNQTFERQDLKAKKWSLGQLDTLIDGGVYFTDTSGSGQRRADIMTKLDQWIDDHGSTP
                     EIPEHLKITSGTQFSEVPVGYKRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVD
                     RYMGNGGDWQRKPGFVTTHKPKVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILIS
                     ESNVMGLGTISYRTFTAEQASLLTYVVGGKLPRP"
     gene            81985..82236
                     /locus_tag="VH752_000161"
     CDS             81985..82236
                     /locus_tag="VH752_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288546.1"
                     /translation="MIKKVTTPSQKTKKRVRNGYLLKLGTACLLLSILSYGIGLLGQP
                     SMENTFMGIASVAMLGSVCFFIIFALNRIFDALEDNLRD"
BASE COUNT        25668 a        16038 c        17446 g        23432 t
ORIGIN      
        1 atcatttttc aacttacttt gtttatgcac tgtcactttg ctatgtgcaa tacaaagtca
       61 tgttatttaa ctcatactaa aaaataatgg ataatagcta tgttagacta gtgacatgaa
      121 gaaatattgc ttttattgaa tcgttagaga atatgatatt tcaaggagaa aaatagtgat
      181 cgagttaaac gatgaattca tccgaaaaga gacgatagaa ctggccaacg atggcccacg
      241 agtacacacc actcaatatg aaactaaagt acctcggctt cataaatgct atctgctttt
      301 ctttagcatt attatcagtt ctttaacaat tgcagtacct tttttaacag atgcagccaa
      361 tggtcttcag tcccaaaact tatatatagg aatgatgtta acgaaggggc aacttcccta
      421 tagtgatact tttacaacag gagggctctt ttactttgtt atcattgcat taagttatta
      481 tttgggatcg acactttggc tagtctttgt tcaggtgttt tgtttttact tatctggttt
      541 atatctttat aaactcatta attatatgac aggctttcaa aaagtggctt taactttttc
      601 aattagctac tatttgttgt ctgttagtct tggttttggg ggattgtatc ctactcaact
      661 ggctatgcca tttatattaa tatcggcttg gtttttaact aagtattttg cctgtttagt
      721 gaaagatgag gcatttattc tttttggctt tgtaggtgct cttgcaatgc taattgaccc
      781 gagtaccctt atcttttggt cttttgcttg tgtgacagtt ttttcttata atataagcca
      841 aaagcatctt gcaagaggtt tttatcaact gctagcttcg atttttggaa tgattttagt
      901 tttttacaca gcaggatatt tcattttgaa cttacaagtg ctaaatcctt atttatcaca
      961 aacgatgatt tatcctttta ctttttttaa atcaggaaac ttatcgttgc tttttggact
     1021 ggctattcag ttgttcttcg ctttggggct tggtcttttg acggggatgg agaatgtcat
     1081 taggcgattt aaaaacaatt ctgatagggt cgtcaagtgg ctatttgtca tggtcattct
     1141 agaatctata cttgtggcta tattttcaca agactatcgc ccctatcatc ttttacctct
     1201 tttacctttt ggattaattt tgactgctat tcctgttggc tatcagtatg gtataggatt
     1261 aggtcagagt agtcatcgca gacgtcatgg taaaaatggt gttggtcgag taatgatgat
     1321 ttatcttaag agacactttt atttgccaat tttaattgta gggacaatac taatctgttc
     1381 tacttattgt ttcattagta gtattcctct taatcaggag cgtgatcata ttgctagtta
     1441 tttagaacag aaactaaata aaactcaatc tatttatgtt tgggatgata cttctaaaat
     1501 ttatttggac agtaaagcta aatctgtttc tcaatttagt tctcctgaca tcaatacgca
     1561 aaaagtgagt catcgaaaaa tattagaaga tgaactatta gaaaataagg ctgcttatat
     1621 cgttgttaac cgctataaaa acctgcctaa aatcattcaa aaagtattat ctactaatta
     1681 caaagtagat aaacagataa cgacaaaaag ttttattgtt tatcagaaaa agtaatttaa
     1741 tatcaatata ttgtgttcga gcaaaaaact tgacacaata tattgatttt tttagttgtc
     1801 gtgatataat agttccgtaa ggagatttga tatggtaagt ttagaagaag acaaggtgac
     1861 tgttcaacct gatattaaag tgattaaacg agatggtcgc cttgttaatt ttgatagtac
     1921 aaaaatctat agtgctttat taaaagcaag catgaaagta actcggatgt cgccacttgt
     1981 tgaggctaaa ttagaggcta tttctgatcg cattatagca gaaattattg agcgttttcc
     2041 aactaatatc aaaatttatg aaatccaaaa tattgtagag cataagcttc ttgcagctaa
     2101 tgaatatact attgcaaaag aatacattaa ttatcgtact cagcgtgact ttgcacgttc
     2161 acaagcaaca gatatcaatt tttctattga taaattaatt aataaagatc aaacagttgt
     2221 taatgaaaat gctaacaaag atagcgatgt ttttaatact caacgagatt taactgctgg
     2281 aatcgtaggg aaatcaattg gtttaaaaat gttaccttcg catgttgcta atgctcatca
     2341 aaaaggagat atccattacc atgatttgga ttacagtcct tatacaccga tgacgaactg
     2401 ctgtttaatt gactttaagg gcatgttagc caatggcttt aaaattggta atgctgaagt
     2461 ggaaagtccc aagtctattc aaactgcaac agctcagatc tcacagatta ttgcgaatgt
     2521 agcatcaagt cagtacggcg gatgcacagc tgatcgcatt gacgagtttt tagccccata
     2581 tgcggagctt aactttaaaa aacatatggc tgatgctaag aaatggatcg ttgagactaa
     2641 gagagaaagc tatgcttttg aaaagactca aaaagatatt tatgatgcga tgcagtcttt
     2701 ggaatatgaa attaatacgc tctttacgtc taatggtcaa acaccattta cttctttagg
     2761 atttggtttg gggacgtctt ggtttgaacg tgagattcaa aaagctattt tgaccattcg
     2821 gattaatggt cttggtagtg aacatcgcac ggctattttc cctaaattaa tttttacggt
     2881 taaacgtggc ttgaatttag aaccagattc accaaactat gatattaaga ctttggcttt
     2941 agaatgtgcg actaagcgga tgtacccgga tatgttatct tatgataaaa ttattgattt
     3001 gacaggatct ttcaaatctc caatgggatg ccgctctttc cttcaaggct ggaaagatga
     3061 aaatgggcaa gatgtgacct caggccgtat gaatcttggg gttgtcaccc tcaatttacc
     3121 tcgcattgcc atggaatcaa atggcgatat ggataagttt tgggagctgt ttaatgagag
     3181 gatgctaatt agtaaggatg ctttaattta tcgtgtcgaa cgtgtcacag aagcaaaacc
     3241 agcaaatgcc cctattcttt atcaatatgg tgcttttgga aagcgtttgg agaagacagg
     3301 gaatgtaaat gatctcttta agaatcgtcg tgcaacagtc tctcttggct atattggcct
     3361 ttatgaagtg gcgtctgttt tttatggtgg tcaatgggaa ggtaatccag atgctaaagc
     3421 ttttaccttg tcaattgtca aggcaatgaa acaggcctgt gagaattggt cagatgaata
     3481 tggttatcat ttctctgttt attcgactcc atcagaaagt ttgacagatc gcttttgtcg
     3541 tttagatacg gaaaaatttg gcattgtgac agatattacg gataaagaat actatacaaa
     3601 ttcttttcac tatgatgtgc gtaagagtcc gacacctttt gaaaaattag attttgaaaa
     3661 agattatcca gaagcaggtg cttcaggtgg ttttatccac tactgtgagt atcctgtttt
     3721 gcaacaaaat ccaaaggcct tggaagcggt ttgggactat gcttatgatc gtgtggggta
     3781 tttgggaacc aatacgccta ttgataaatg ctataattgc caatttgaag gtgattttac
     3841 cccaacagaa cgtggtttta cttgcccaaa ctgtggcaat aatgacccta aaacagttga
     3901 tgtggtcaaa cgtacatgtg gttacttggg gaatcctcag gcccgcccaa tggttaacgg
     3961 tcgccataag gaaatctctg cgcgtgtaaa acatatgaat ggttctacta taaaataccc
     4021 aggcctgtaa actcaagctg ggggagagcc cttttgatgg aaatatgata tgcttaaaag
     4081 gttgcttcat gcaagcaatc ttttattgtc taatggtttc ttagcaattg tgagataaga
     4141 ataactaatc caaaacgact aattagctgt tagcaatcgg actataagaa gaaagagata
     4201 aggaagaaat aatgggaaaa tatcaattag actataaagg aatgcagcag gtagaacgct
     4261 ttcatgagaa gcattctaaa aaaaagacgg ataaaaaatc ccgcgttcaa gaacttaagg
     4321 ctcggttttt agagaagtca aaaaaacagg ataactaaag tatccaataa agaaagaagg
     4381 ttgagtatgc ttaatattgg gattgtggga ctaggtgcca tttcacaaaa agcctattta
     4441 ccatatatga gacagctgag tgacattact tggcatctat caacgcggaa tgcagcagta
     4501 cgtcagcagg tcggtcagtt atttggtcac gctattcttt acagtgatgt caaggagttg
     4561 tcaaaaacaa acttagatgg ggtctttatt catgctgcga caaaagctca tgttgatcta
     4621 gcaagcttat ttctaagaca aggtattcca gtatttatgg ataagccatt ggcagataac
     4681 tacgactcag taaagtatct atacgagttg gctgaagctc atcacacttt tttaatggct
     4741 ggttttaata ggcgctttgc accatgtgtc aaggaattgt caagtttgtc aactaagcgt
     4801 aaagtggcgg ctgaaaaaaa tgacttgaat cggccaggag atatgacttt taagctcttt
     4861 gatttcttta tccatccttt agatacagcc ttattcttga cagaagggac cttactaaaa
     4921 gggcattttc aatatcatct ggaagcaggc ttactgagcc aagttatggt aaccttgatg
     4981 accgaaagca tgacgactac agcttctatg aatttacaat cagggagtcg ccgtgaggta
     5041 atggaagttc aacgggctga agaaacttat catttagaga acttagacga gttatctatt
     5101 tataaaggta ctgaaaaaag ggtgcttggc tttgcttctt gggacactac cttgcacaaa
     5161 agaggttttg aaacaatgat cgatgccttt ttagaagcta ttagcacagg cgtcaatcct
     5221 gtcagccctg agtctagtct tttaagccac tggatttgtc aacagattgc tgactctcag
     5281 ctttcttatg gagagttaac ggtagagttg cctaaggatt aggaattgat atggaaataa
     5341 gacgaccgac tttaaaagat aaagatgctg tattgtcaat gattaacgaa tttttagagc
     5401 agaaaagtgc tacagatggg ttatggcatt ttaacgtcaa tgacttcaac tatgaaacat
     5461 ggttggaaga ttccttgcga caggaaatgg gtttggccag tcaaggagtg cctgctattc
     5521 aatatgtggc gtttgatgag aaaaatcaag ctttgggatt tttaaacctt aggttacgtt
     5581 taaatgcctc gttacttgaa aaaggtgggc atattggtta ctctgtccgt cccagccagc
     5641 gtgggaaagg ttatgccaag gagatgctta aacaagctgt cagctgtgcg atttccaaga
     5701 atatcacgac aattttggta acttgtgatg agactaatgt tgcaagccga gcagttattg
     5761 tggctaacgg cggtgtcttg gaagatagtc gaggtggaac ggaacgttat tggattgaag
     5821 aacaaagagg cgataagtga tggcagaaaa gtgttggaat aatcctaagc caaaggaatg
     5881 gcaggcagag gagttaagtc aaggccggat tattgactat aaggctttta actttgttga
     5941 tggtgaaggg gttcgtaatt ccctttatgt gtctggttgt ttattccatt gcaaggggtg
     6001 ttataatgct gcgacttggt cttttaaggc gggaatgcct tatacgcaag aacttgaaga
     6061 gcagataatg acggatttgg cacagcctta tgttcaggga ctgacacttt taggtggaga
     6121 accctttttg aatacgggta tcttaattcc tctgattaag cgtattcggt gtgaattgcc
     6181 agaaaaagat atttggtctt ggacgggtta tacttgggaa gaaatgatgc ttgaaacacc
     6241 agacaaactg gaaatgctgt ccttaattga tattctggta gatggtcgtt ttgatatcac
     6301 aaaaaagaac ctcatgttgc aatttagagg ctcttctaac cagcgaatta tcgatgttca
     6361 aaagtctttg gctgctaaag aagtgattat ctgggataag ttaaacgatg gggaccaaac
     6421 ctttgaacaa attagtcggg aggatttgtt ttaagaagct gacttatcca catgattttt
     6481 ttgaaaaggt catgtgaaaa caggtccttt aagggtgaat tggggagaaa gtaattgtta
     6541 ttccctggtt ttccccacat tatcctcata gttatccaca ggttgtgtat aagttgtaga
     6601 cttgtgaata aagactaaaa aagaggccta ggcttctttt ttgatataga caaattcatg
     6661 atctgttgat aggtctggat ggtagtaaaa accagcaaag acaaggcttt taaggctgtc
     6721 aacagtttgg caattatttt ccatacaggc agtgagaaat gcacctcgtg ccttttttga
     6781 aatggtggaa tgggttttga atcggccctc tttttctgcc ataaacttgg gacttatcca
     6841 caattgttta cagtctttgg aaaagacatc gtcaaattca ctggataaaa gggaaatcac
     6901 ttggggatgc tctttggcaa actgattata acagggccgc cagtaagact tgaggctttg
     6961 tccttcaatt tttattctgg tgtgaaaatc atggcggtgc tctgcgatag ggtgattagc
     7021 gggaatgata ccgtaaaagg atgaggtgat gtaaacctgc tgggtgaggt aagcctgttc
     7081 ttgggtagtg agcttatcgc gcttgatatg gcggtacata aggccattga acagttggta
     7141 ggcagggtaa gcgaggcttt gttgagaggc catgtcttgc cagcgttgct gttctttttt
     7201 agctgcctct tctttgatac gatatgattt ggccaagtcc tctgtggtca tagctgccat
     7261 tgcctttaaa atggcctggc ttggttgggg cagtaggtgt ggataagatt ctttaggaat
     7321 caccatttct tttgcggttg gaattaaaaa ggttagcata gtgttattgt agtaaagtta
     7381 cttgctttct gtcaagaaaa ctagtcttgt ccaaggatga cgtccacacg gataccttcc
     7441 tcgtcttctg tcatgaaaga agaagaatct tcttgtaaaa tagctaggcc atatcatctg
     7501 gcttttttga gagtagctga aaaaagcagg ggcgtctcaa tgtgaatggt taagaaggct
     7561 agacctggag tgccttcttg gtgttttttg aggtaagggg ctgaccagtg attgaatgca
     7621 aggtgatgat ggtagttccc tgaagcaatc cagctagcac ttggaatggt cattttatcg
     7681 cctaaatcaa aaaccttttg atagagaagt gaagaatcca gagcattttt gacactcaga
     7741 tggacatgac taatgcgtgt gtcttgagct agtaaaaagt gcttggggat atctgtcaac
     7801 tgctccaata tgcttttggt atccgtgggc tcagtcacac caataatttg accattgtcg
     7861 cggatatccc actgctcaac agctttgtca tgatagattt caatcccatt accctcagga
     7921 tcgctgaggt agatagcttc gctatagccg tgatcagctg ctccttctaa agagatacct
     7981 ctggttaaga aatgaagtcc caagctatga cgatcaggaa ctaaaaaggc tgtgtgataa
     8041 agaccataag ccttatttgc tggtaaaggt gtttgtcgta actctaagat aacggtttta
     8101 ccgtctgtag tcaattgacg agatgtcgtg tcttgtgaga gaacctgtaa accgataata
     8161 cttgtgtaaa aggttgtcat ctttgctaga tcggtaacat tgagggaaac ctttcctaga
     8221 gagatggttg agttgtaagg gtatgtcatc ctattgtctc caaaagtgat taagcctatg
     8281 ataacttatt agtttcaaaa agaaaagtag gcacaaataa gtggtatagg cactaaaaag
     8341 taactcattt gtcagcccta tcttgtttct gttctctcta atagtctata atgggttata
     8401 agttacaaaa agatactaag aggagctgct gcctatgctg aaaaaaagac cggcttgtcc
     8461 cgtagaaaca accttatcgg taatcgggaa taaatggaaa ttattaatat taagggactt
     8521 gctaaagggg accttgcgtt ttgggcagtt gaagtcctca ataggttcgg tcagtcaaaa
     8581 ggtacttact gcccaattaa gggctatgga agcagacgga ttggttcatc gggaattgta
     8641 tgcagaagtg ccacctcggg tggagtattc cttaacagaa actgggttga gcttagctcc
     8701 tgttattgaa gcgatgtcgg attgggacca aacttaccaa gaaaaacatg ccgatttatt
     8761 tgaagaaggc tagaggaaag agttgtccga gcatactgag acttagagaa gctgagagtt
     8821 tttttaaaat gatattatca aattctttac agtttgataa aattactttt tttctacaaa
     8881 aagacttgcg ctcatctcac gaatatgata atataatatc aagtctgttt gagaaaacag
     8941 aagctcaggt caagtaagtg cacacttaca ataacacagt tgataatggg gaaactcaga
     9001 gtcaagaaaa agaaaagcaa gccttgcatt ttgcaaggct tttttctgtc tttattttgg
     9061 tctgcgccga gaaaaatcta aaaatttaaa tttttccagt ttatggcgac tctcggtgaa
     9121 ttggaattgg ttattattgg aaaggtaaac tttggatttg acggaaacaa catgttggtc
     9181 tgatccaagg tcaagtaaaa ttttatcacg atcggttatt tggtcaatgg taacctcttt
     9241 gagggcaaag tcaatggcga gatggagttc cttttccaaa tattgataaa tagaatgctc
     9301 tgcgatttcc cttgtcatta ttggcaccaa agttttacta aggtaatcaa tgtctaagac
     9361 ggaagccaca ccctcaacaa cgcgttggcg cgtaacacgc cagaccaagc tgtttttgct
     9421 aaaaccggtc aattttgaaa gtgtctcatc cacaatcagt ttatcaatgg caatgacgtt
     9481 ggtcttagaa tctagatttg agtaagagac gagttcctga taactagtta gttctgagat
     9541 agggaacatg atctgatgat gtttaatcac ttgggtgcca cgtccttgtt ttttgaggat
     9601 aagacctgcc tttgtgagaa gtgagagagc cttgcggacg gtatctcggc tagcttggta
     9661 ttgttgactc aattctattt cagtaggtaa aaaatcccct tctttgtaaa agtctttgtt
     9721 tatttttgtt tcgaggtctt tataaatacg ttcatacttt gtcatagggt aaattacctt
     9781 tccacttgtc tattttttta cagacaacac cttttatttt accaaaataa tggctttttt
     9841 ggcacaaaca acttgcagac aaatttgcaa tcgattacaa taaaggtgtc aatcatattt
     9901 tatctaagaa tttgaatatt tcattttgac gttgcttgtt aaaagcaact agaacaaagg
     9961 actaggggat gtcacatggg aaaatttgaa caggatgcta aaagtcttct aactgctatt
    10021 ggcggtaaag aaaacatcaa ggctgtcaca cactgtgcaa cgcgtatgcg ttttgttttg
    10081 aatgataata ataaggcaaa tgtcaaagag attgaaaaaa tctctgtagt taaagggaca
    10141 tttaccaatg ctgggcagtt tcaggtaatc attggtaatg atgttccagt tttttataat
    10201 gactttacag ctgtttctgg tattgaaggg gtgtctaaag aagctgccaa atcagcagct
    10261 aaaagtaatc aaaatgcctt acaacgggtg atgaccatgt tggctgagat tttcacacct
    10321 attattccgg cgattattgt tggggggctt attttaggtt tccgtaatat tttggagagt
    10381 gtgccttttg aatttcttgg gcagcaggtc gaaaaaggga aattagtttt tgatgcagct
    10441 ggggatcctg tttggaatac gattgtgagg gtatctcctt tctggtcagg ggttaaccat
    10501 ttcttgtggt taccagggga agctattttc cacttcttac cagttgggat tacttggtct
    10561 gtgacgcgta agatgggaac cactcaaatt ttagggattg tccttggtat ctgtttggtg
    10621 tcaccacaat tattgaatgc ctatgcggta gcaggaacgc ctgctgctga gattgccaaa
    10681 aactgggttt gggattttgg tttctttacc attaatcgta ttgggtatca ggcacaggtt
    10741 attccagccc ttttagctgg tctgtccctt gcttatcttg aaattttctg gcgtaaacgg
    10801 attccagaag tggtttcaat gatttttgtg ccattccttt ctttgattcc agctttgatt
    10861 ttagcgcata cggtattggg gccaatcggt tggattattg gtaaagggat ttcctttgtt
    10921 gtgttagctg gattgactgg tcctgttaaa tggctattcg gtgctatctt tggtgccttg
    10981 tatgctccgc tagttattac tggtttacat cacatgacaa atgccattga tacccaatta
    11041 attgctgata ctgcaactcg tacaactggt ttgtggccaa tgattgctct ttcaaatatc
    11101 gctcaagggt cagctgtttt tgcttactat ttaatgaatc gtcatgaaga acgtgaggct
    11161 gaaatatcgc ttcctgcagc aatttctgct taccttgggg taactgagcc tgccttattt
    11221 ggggttaatg ttaaatacgt ttatcccttt gtagccggaa tgattggctc aggtattgcg
    11281 ggtctcttat caacaacctt tgatgttcag gcaaattcta ttggtgttgg tggcttacca
    11341 ggtttcatgg ctatcaatgt gaagtatatg attccattct tcatctgtat ggcagtagcc
    11401 attgtggtac cgatgttttt aaccttcttt ttccgtaaat cacatatcat gaccaagaca
    11461 gaagatgagg ctaaactagc tgagacaccc gtttcggagg ctcctgcggc aactgctcca
    11521 cataagacta tgcaaggaac agttatcact ttaaccagcc ctttaacggg tgaagttaaa
    11581 gcgttgtctg aagctgttga tcctgtcttt gcacagggag ttatgggcca aggtgttctt
    11641 cttcaaccga cagaaggggt gttagtagcg ccttgtgatg ctgaagtatc ggtcttgttc
    11701 ccaaccaaac acgctattgg tttggtgacg gctgaaggtt tggaattatt gatgcatatt
    11761 ggtatggata cggttaactt agatggtcaa ggatttgaag ctttggtgaa gcaaggtgat
    11821 caggttaagg ctggacaaac attgattcaa tttgatatag cagctatttc tgaagctgga
    11881 tacgccactg aaatgcctct tgtggtgact aatcaagata cttttacggt aactgttgaa
    11941 ggtagtttac cgcatcagat taaggctaat gataagctag tagtagcggt gaaaaagtag
    12001 gtttactaag agaaaacgtt caggaactga gatcagttcc tgaaactatt ttagggagga
    12061 aaactcatga caattgataa aaagaaagtc gtctatcaaa tttacccaaa atcctataag
    12121 gacactactg gaaatggtgt gggagacttg cgagggatca ttgataaatt gccttactta
    12181 caagaactag gaatagatat gatttggttg aaccctttct accctagtcc acaacgagac
    12241 aatggctatg atgtttcaga ttatacggcg gtcaaccctg attttgggac gatggctgat
    12301 tttgaagatt tggtaaaagc tgctaaggag catcagattg agttaatgct ggatatggtt
    12361 ttgaatcact gttccacaga ccacgagtgg ttccaaaaag ctttagcagg agacccttat
    12421 tatcaggatt tctttatctt gagagatcag ccgactgatt gggtttccaa atttggtggg
    12481 aatgcttggg cgccttttgg agatacaggc aaatactact tacacttgtt tgatgtgaca
    12541 caggctgact tgaattggcg gaacccacat gttcgtgagg aattggctaa agtggttaat
    12601 ttttggcgag ataaaggagt gaaggggttc cgttttgatg tgattaacct gattgggaaa
    12661 gatgaagagc tggtggattg tccggtcaat gatggtaagc cagcttatac ggatcgtcct
    12721 attactcaca cttatcttca tgatctcaat caagccagtt ttggtcagga tgattcgttt
    12781 atgacagtag gggaaatgtc tgccacgact attgacaact gtcttttata cacggctccc
    12841 gaacgggaag agttgtccat ggcttttaat ttccaccatc taaaagttga ttatgagaat
    12901 ggtcagaaat ggactattat ggcttttgat tttgcagcgc tgcgagactt attccatgcc
    12961 tggggtgaag gcatgagtca aggcaatggg tggaatgcct tgttctacaa taaccatgat
    13021 caaccacgtg ccctgaatcg ttttgttgat gtaacacatt tccgaaacga aggtgcaacg
    13081 atgttagcgg cttccatcca tttgtcacga ggaacgcctt acatttatat gggtgaggag
    13141 attggcatgc ttgatccaga ttttgatagt atggataatt atgtggatgt ggaaagtctc
    13201 aatgcttatt caagcttact agtctcaggt aaaagtgcag aagaagcctt tgccattatc
    13261 aaggctaagt caagggacaa tgccagaaca ccaatgcaat gggatgctag tgaacatgct
    13321 ggctttacga ctggtaagcc ttggttagag gttggcaaat cttatcgaga catcaatgtc
    13381 gaaacagaaa aagagggacg tatttttcct ttctaccaac gcttgattgc tttgcggaag
    13441 gaactcccta ttattgctga aggggactat cgggctgctt ttaaagatag tcaggctgtc
    13501 tatgcctttg aacgccattt aggtgaccag tgtttgcttg ttctcaatca tttctatgct
    13561 gatgaggtcg aactggaatt acctccacgt tatcaacatg gacaggtctt aatcagcaac
    13621 tatgagaaag tttctatttg tgaaaaagtg atactgaaac cttatcagac acttgctatc
    13681 ttagctgata actaactaaa agcctgaggt taggcctcag gcttttggtg attgtcacga
    13741 aaaaagaagt tttctcatat atttattttt aaactcttat tttttctaaa taaaaacaat
    13801 tgagacaagt tattttagct aaattttggt acaatggaaa agattataag tagagtttaa
    13861 gataaagatt ctacaaattt aaaatgatat aaggagatac gaatgacaac ttatcaagat
    13921 gatttttacc aggctgtgaa tggaaagtgg gctgaaacag cagttattcc agacgacaag
    13981 cctcgaacag gtggattttc agacttagca gatgaaattg aggcattaat gctagacact
    14041 acagatgctt ggctagctgg agaaaatatc cctgatgatg caattttagc aaactttgtt
    14101 aaatttcacc gtttggtggc tgactacgct aaacgtgatg aagttggagt aagtccaatt
    14161 ctccctttaa ttgaagaata tcagtcctta aaatcttttt cagaatttgt tgctaatata
    14221 gcgaaatatg aattggcagg tttaccaaat gaatttccct ttagtgtggc accagacttt
    14281 atgaatgctc agctgaatgt gctttgggca gaagcgccaa gcattctgtt gcctgacacg
    14341 acttattatg aagaaggtaa cgaaaaggcc gaggaattac gtggtatttg gcgtcagtcg
    14401 caagaaaagc ttttacctca atttggtttt tcaacagaag aaatcaagga tcttttggat
    14461 aaggtaattg agctagataa gcaattggcc aagtatgtct tgtctcgtga ggaaggctca
    14521 gaatatgcca aattatacca tccttatgct tgggctgact ttaccaaact agctccagag
    14581 cttcctttgg atagcatttt tgaaaagatt ttaggccaag tacctgacaa ggttattgtc
    14641 cctgaagaac gtttttggac agaatttgca gccacttact actctgaagc taactgggat
    14701 ttactcaaag ctaatttgat tgtggatgcg gcgaatgctt acaatgctta cttgaccgat
    14761 gagattcgtg tacagtctgg tacctatgcg cgtgctttat ctggaacgcc tcaagccatg
    14821 gataaacaaa aagcagcctt ttatttggct caaggaccgt ttaaccaagc gcttggcttg
    14881 tggtatgctg gtcaaaaatt ctccccagaa gctaaggctg atgtggaaag caaggtggca
    14941 cgcatgattg aggtttataa gtctcgtcta gaaacggctg attggttagc accagccaca
    15001 cgtgaaaaag ccattactaa attaaatgtc atcacgccac atattggtta tcccgaaaaa
    15061 ttaccagaaa cctatgccaa aaaagtgata gatgagactt tgtccttggt tgaaaatgct
    15121 caaaacttgg caaaaatcac gattgctcac acttggagta aatggaacaa gccagttgat
    15181 cgtagtgaat ggcatatgcc agcccacttg gtcaatgctt attatgatcc tcagcaaaat
    15241 cagattgtct tccctgcggc aattttacag gcaccgtttt attctttaga tcagagttct
    15301 tcagccaatt atggaggaat tggtgcagtc attgcgcatg agatttcaca tgcctttgac
    15361 accaacggtg cttcctttga tgaacatggt agcctaaatg attggtggac acaagaagac
    15421 tatgctgcct ttaaagagcg tacagataag attgtggcgc agtttgacgg cttagagtca
    15481 catggtgcta aggtcaatgg taaattgaca gtttcagaaa acgtcgctga tctaggtggg
    15541 gtagcttgtg ctttagaagc ggcacaatca gaagaagact tctcagcccg tgacttcttt
    15601 attaactttg cgacgatttg gcgtatgaag gcccgtgagg aatacatgca aatgctagct
    15661 agcattgatg ttcatgcccc gggtgaactg cgaacaaata tcaccttaac caactttgat
    15721 gctttccatg agacctttga tattaaagaa ggcgatgcta tgtggagagc tccgaaagac
    15781 cgtgtgatta tttggtaatt aggtagttga taaaccttca aatgcctgtg attataaaaa
    15841 tgactgattc tgtgaaacac aggatcagtt ttttgcttcc ttattggtgt ccgttttgat
    15901 acaacaaaaa gacaaccttc agggaaagaa ggttgccttt tttagactta tcagaaataa
    15961 gtgattagtt gttaagggca gcagccatag tagctgcaac ttctgattca aagtcgttag
    16021 cttttttctc gataccttca ccaacttcaa aacgtgcaaa agcaatcgct ttagcgttta
    16081 ctgagtcaag gtaagcttca acagttttgc tgtcgtccat gatgtaaact tgtgcaagaa
    16141 gagtgtaagc ttggtcaact ttagtgttgt caagcatgaa acggtccatt ttacctggga
    16201 tgattttgtc ccagatcttt tctggtttgc cttcagcagc aagttcagct ttgatgtctg
    16261 cttcagcagc agtaatcacg tcgtctgata attgagcttt tgaaccgtat ttcaagaatg
    16321 gaagtgctgg tttgtcaacc attgcacgcg attcgttgtc aagttcgata gcgtggttca
    16381 attgagcaag ttcatctttg atgaattgtg catcaagctc agtgtatgaa agaacagttg
    16441 gtttcattgc agcgatatgc attgatactt gtttagcaag agcatcgtcg ccgccttcga
    16501 caactgagat aaccccgata cggccaccat tatgttggta agcaccgaag tgttgctcat
    16561 ctgttttttc aatcaaagca aaacgacgga atgagatttt ttctccgatg gtagcagttg
    16621 cattaacgta agcttcagca agagtttcac ctgaaggcat aacaagtgca agtgcttcat
    16681 cgttgttagc tggtttgcct tcagcgataa ctttagcagt tgcgtttacc aattcaacga
    16741 attgagcatt tttagcaaca aagtctgttt cagcgttaac ttcaataaca gcagcaacgt
    16801 taccatgaac gtaaacgcct gttaaacctt cagcggcaac acggtcagct tttttagctg
    16861 ctttagccat tcctttttca cgaagaagtt caacggcttt gtccatgtca ccatctgttt
    16921 caacaagtgc ttttttagcg tccataacgc cagcaccaga tttttcacgt aattctttta
    16981 caagcttagc tgtaatttct gccatgtgta gtatcctcca aatgatattt gttttggttt
    17041 caaaaaacga gacagagcgg tttgctttct gccccgtttg gttttcgatt gttaaagaca
    17101 aacaaagttt gccataagag ttaggaactc tgtgctaatt aagcgttgtc gccttcgaca
    17161 acttcaacga tttcttcgat tgaatctgct tgagtgtcag cttcaaaagc aacgtctgca
    17221 tcttcacctt gacggccttc gataatagca tcagctaatt tagcagtgat taatttaacg
    17281 gcgcggatag cgtcatcgtt agctggaatg atgatatcga tatcatctgg atcagcgttt
    17341 gtatcaacca tcgctacgac tgggatacca agttttttag cttctttaac agcaatttgt
    17401 tctttatgtg ggtcaacaac atacatcacg tctgggatac gaggcatatc ttcgataccg
    17461 cccaagaatt tttcaagacg agcgcgttgt ttgttaagaa gtgcaacttc tttcttagga
    17521 agaacgtcaa aggttccttc ttcttccata cgtttgattt ctttcaaacg agcgatacgt
    17581 ttttggatag ttccccagtt ggtaagcgtt ccacccaacc aacggtggtt gatgaagtat
    17641 tggcctgcac gcgttgcttc gtcagcaact gcttcagcag cttgtttttt agtaccaacg
    17701 aacaagatga cagcatcgtt tgctgcagca tcacgaacaa attcgtaagc ttggtcagct
    17761 aattttacag tttgttgaag atcgataacg tggataccgt tacgctctgt gaagatgtat
    17821 ttagccatct tagggttcca gcgacgagtt tggtgaccaa agtgaacacc agcctcaaga
    17881 agttgtttca ttgaaattac tgccatgagt agtttctcct ttataaaatg ttttttcctc
    17941 ttccagactt cgacttgcag gctaacctag cggcaacaag cccacaattg gtctagaatg
    18001 agtatttgcc gtctacacga ccctactata ttatcaaaaa aagaggttaa aagcaaggag
    18061 aaatactatg gattccttta tttttgagat gactagcttc tttgtttatt ggtaagtgtt
    18121 aagacaagct tttgctcatt ttgggtgaca cttgccaaat cgttttattt aagatatagt
    18181 gaatttgact aatttttgga ggcgttttca tgacgaaggg tattcgattt caattgcttg
    18241 gcagtcccca catttttctt gatgacaaag aacaattttt tgcctttgcc aaagcaaatg
    18301 ctctccttta ttacttagtg gttaatggat ctgttagccg cgaggtggcg gcgagtctac
    18361 tttgggaaaa taagaacatg caaacagcga aaaagaattt acgaaacgct atctaccagg
    18421 tcaataaagt cttgcaagct gatgtgatta tttgtcccaa tcgcaacctg ctggttttga
    18481 ataaaacact tgatatcaaa acagatatta acctttttct ggctgatccg ctagctcact
    18541 tagaactcta ccaaggggag tttttgcagg gcttttacct taaaagtggt gaggaatttg
    18601 atctttgggt ttcaaaaatg cgtatgcagt atgaacaggt atacctaaaa gcgtgttacc
    18661 aaaaaattga agaaaagctc tctttagatg ccattgaaga tgtcgaggaa catttaaaac
    18721 agcttattga acgtgatgaa tttgaagaga aaaattatca gctcttgatg aggctttatc
    18781 agcaggggaa ttgtcctggt aaggtcatcg aaacttatta tcagttggct aatgtgttgg
    18841 ataaggagct agggattcag ccaagtttgc aaagccaaca gatttatcag gaagtggtcg
    18901 ctaaagatcg taatgaacgc aaaatcaagc attttttgcg caacagtaat cattttttgg
    18961 ggcggattga tgagattaag cagctggaaa actattttgc caactgtttg gcctgccaag
    19021 aggtaggagc tctgctctta attggtgata cagggattgg gaaacgaacc ctagccagac
    19081 aggtcttagc caatcaaacc caaacgtttc aaattgtcac agctaaatgt tttcgtgaag
    19141 aggctatgga ttccttattg ccttggcgta atatcttaga tggcctggga gatttggtga
    19201 ttcaaaaccg cttattgact accaaagctt ggaaggctgc tcttaaacgc tgttttcctg
    19261 tggcaaccat ttttcaagaa gataacaacc aaccctttat caaggaccac accagcttgt
    19321 tggtttcctt tattgttgat attttacaac atttggcaga aatcaaggcg ctggtaatcc
    19381 ttattgagga ttgtcactgg atggatgagg atagtttgac cttgttgcaa cgggtcatga
    19441 atcaattggt ccactatccg attgcttttg tcttgaccaa gcatctagga acaactcctg
    19501 agctcggtct ttgtttgaat gctttgatga gtcaggggcg tttagaaagc atttgtttgg
    19561 agccttttaa tcggcaagaa agtttggctt atattaacag tcaactgggt agtcagccag
    19621 tgacggcaga agaaatggag cacctctatc aagccagcca aggcaaccct ttctttttgt
    19681 cagaatacac tcaggcccta ctgcgtcacg aaaaatttgt gcctttaacg cctgccatta
    19741 aggctaagtt gggtcttaaa ctagctaatc taagtagtcg tgatgacgca ttgttaaact
    19801 atttgtcttg ttgtcggagg cctatccctc taaataccct cgctcaattg atgttactgc
    19861 ctttagaaga agtgatcgag atggtggata atctggggca ctactatatt ttggtagaag
    19921 aaagcgtagg agaggaagtg ttgatttcat ttcgtcagcg aattattcaa ctctatagtt
    19981 atgaccgttt atctttgtca aaaaggcgtt tattgcacgg acaaattgcc aaacggttag
    20041 aagatttact gcctattttg acaccaagcc ctcacttatt agatgacatt gcttaccatt
    20101 atcaggaatc acggcaggtg attaaggctt tggaatacaa ccttaactat ctagatgcta
    20161 ccttgccatt tcagcatgag ctgtttccca tttattcaaa gagtattggc tccttggaaa
    20221 agtcagatcg tgaccatcaa cgcttaatgg aagagcagtt tgataagatt cggcaaagca
    20281 ttgcggattt ggaactgacc tatgataata accgtgattt ccagcagttg cttattcgtt
    20341 tttcttacct tgaaggccgt tatgatattc gaacagggag gtatcaagaa gggattaaac
    20401 atattcaaaa ggtcatcgct ttggcaaccg agctaaaaca gccctccttc ttgctagaag
    20461 gctatcgcca gttaattcat tactgtatcc aagtggaaaa caagccagaa atgcgttatt
    20521 acactagtct ttcattggag gcagcggttg ctgctaatca ttttgaggcc attgcgatta
    20581 gtttgcggtt aaacggtctt tatcatttga ttattgggga gttaaatgag gcagagcgtc
    20641 tgcttcaaca gtcgattgac ttctttaagg tgacgccagg cttgcaagct aactatgcga
    20701 ttcagattgc tgctgcctta gattatttgg gggagattgc ccagattcgc tatcagtttg
    20761 aaaaggccgt tacttaccaa aaacaagcca ttgccttgac cgaaaacaag ccagctgagt
    20821 tatcggtgag tattttttat attggattag ggatttccta cttttattta gctgattttg
    20881 aacaagctga gcaaatcttg agtttggcta aggaagcttt ggtcaatcat agttatcctt
    20941 ggaaagaaac gcaacttgaa atttacctgg ccatgattca atggaaaaag ggtaattatc
    21001 agccagcctt gaccctactt gattacagag aaaccttgat gtctaggtat cgcaatccgc
    21061 gtgataaggg cttggttttc tatttgatgg ctgtggtcaa gtaccaattg atatttcaag
    21121 gagctaccct aagtcagcag gaaaaggaaa tggcagacca cctgttgtca gaatcctttg
    21181 agtattatta tgagattgct agtactaacc tcaaccctta ccgagattgt catttggtga
    21241 gtgagttgaa cgatttgcgt caacaattat ctgctaaaag ctaaggaaaa ccctctgatt
    21301 cacatcagag ggttttagct atgttgtgcc ataatctgta cgaggtaaaa gataaaggtg
    21361 gctgctaaat tggcggtgtg gttatcaata tcgtgtggtg gtgaaacctc aaccacatca
    21421 aagccaacta attttctact agccgcaatg tgttgcaaaa ctaagacggc aagatttggg
    21481 tcaacaccga gggactggat agcactaacc ccaggtgcag caccaactga gaaacaatcc
    21541 atgtcgatag tcaggtagac cctttcttgc ccttctaaga aacggtcaat ggctcggcag
    21601 accttttgat gccccatttg gtagatgtct tgaccagtca aaaattgaat gcccttggat
    21661 tttgccacaa agtcaaatag gaaaaggttg ttattatgtt cttgaatgcc taaaacgaag
    21721 tatttaaaga ggcgtttgtc agcaaccgca tcgtcaaaca tttgtcgaaa gcctgtccct
    21781 gaattagggc ctgtttggtc gtaaggcctt aaatcaaagt gggcgtccat attgatgaca
    21841 gctaggtcat cacttgggga taggctttgg cgtaggccta agtagtggcc gtaggcggtt
    21901 tcatggccac ctcctaagac gatgggtttg agattgaggt cacacatccg tttgatggct
    21961 ttgcttaaac tgttttgcag ttgttccaaa gagcgattgg ggccgtcaat gttacccaca
    22021 tcgtaaacca taacttggtt tcctaaatgc caagggaatt tggcgagctg ggttcgtatg
    22081 gcagctggac tttcgaccgc tccgacacga ccattattga tataaacccc cttatcactt
    22141 ttaaagccaa tcaaggcaaa atgtgtccct tcaaatgggg ttagtgagct gtcgttaagg
    22201 tcaaggaaag tcatgaccat gccccatttg gcagtatata agtcatcgtc gatcccacca
    22261 tgataatagc tagtggtgga tgggtagtaa tcttctagca tgcgcgtcat ctcctttagg
    22321 attatgatag caaaataaaa cttacagtca acactttctt ggcctttgtc actggttttt
    22381 aggaatagca gagcaataaa atcgtctgct tttggtcccg gttagttagt ctaaagaggt
    22441 catgacctct gccacagccc ctctagttaa agaagtacct tattcactaa aattggatac
    22501 tcaactcaac tgtctgctca accgcttcga ggaagcttgg gtcttcaatg actgttgatg
    22561 ccttgtttag ttcatcgtag atttcgatgt gtttatcgtg ttcgataaag ttgacttcct
    22621 tgcggaagag atcgtaggca accttagtcc ctttacctag ttcatgattt tcagatttga
    22681 gatcaagggc ttggcaggct gccatgattt cagtagccac gatgcggcga gaatttttaa
    22741 ggatttcaaa ggccttgcga gcagcggtgg tgcccatgct gacaaagtct tcttggttct
    22801 cacaagatgg gatagaatcc acactggctg gatgtgccaa aaccttgttt tcagaagcaa
    22861 gagaggcaca ggcgtactgg gtaatcataa agcctgaatt gagacctggg tatttgacaa
    22921 ggaaggatgg caatttgctg agctggctat tgaccaaacg ttccacgcgg cgttcagaaa
    22981 catttccaat ttcagaaatg gcgatgccta agaagtcaaa tggctgtgcc attggttctc
    23041 cgtggaagtt acctcctgaa ataacgtggc catctttaca gataattgga ttatctgtga
    23101 cagaattgat ttcaatgtca actttggatt tgacataggc gatactgtct ttgctagccc
    23161 catgaatttg agggatgcag cgcaaggtgt aagggtcttg cacacgagat tgagtagcta
    23221 cggttgtgtt ttggctacct tctaggaggt ttcggatatt gcgggctgtt gccaactggc
    23281 cgctttgagg gcgaatggtg tgaaggtttt cttcaaatgg actagtaatc ccgttatgca
    23341 cttcaagaga tagggcacca gctaggtctg acagtttcaa taattgaatg gcgtcatagg
    23401 ttgcaagggc accgattgca gtgagaacag ttgttccgtt gataagggcg agtccttctt
    23461 tagcggctaa agaaatcttg tcaataccag ctttatcaag agcttcttga ccagaaagaa
    23521 gctctccttt gtagtaagct ttccctagac caagcatagg aagcaccatg tgagcaagcg
    23581 gcgccaaatc tcctgaagcc cctagagacc ctttttcagg aatgtaagga tgaacccctt
    23641 tattgagcag ttccaacagt ttttcaatgg tggatagacg aataccagaa taccctttga
    23701 cgagagagtt gatacggatc agcatgatag ctctcacggc atcttctggc aatgggtcac
    23761 caaatccact agcatgggta cgaatcaaat tttcttggag ttgaactgta tcttctggtg
    23821 agatgcttac gttacaaagg gatccaaaac cggtggtcac cccgtaaacc acccgttttt
    23881 cactgacaat gtcatcaaca attttacgag aggcattcac agcttcaata gcactatcat
    23941 caatgcgaca ggctacgcct tggcgagcaa tcgcaatcac gtcttcgata gtaaggcttt
    24001 cgccatctaa attaatcact cttgtcatag tttttctcct agatttgttc cttagggggc
    24061 atttggtcca tttggtccat ttggtggtta cgtccgtgca taaggtagta cacactactt
    24121 gcaatgatga caccaactgc accataaaca aagttcattg ggttgtcacc ccaaatcata
    24181 actaaactaa cgataactgc aagaattggg ataatagggc caaatggtac tcggaagatc
    24241 acattagcat cgggatcgtc cttccgtaat ttcataacgg ctagtgctgt tggaatgtat
    24301 tggaagaagc ggaaaacaac actgagtttt gctaagtttt caaatgagcc agtgagtagg
    24361 agtataatag caattgctcc tgaaaccaag atagcaacga gtggcgcgcc attttgattt
    24421 tgcttggcaa tagcagctgg taagagccct tcatcagcaa tggcagcacc gtaacgtggc
    24481 accatgatgg attctcccat gttaagacct gtaatggaaa ttaaagcacc gattgagacc
    24541 atccaagcgc cagcagggcc tatcatttta acaaaggcat cttgaacagg tgcattggtc
    24601 atcatgattt gagagccaag catggcaatg gttccaccga taatcagcat atataagaca
    24661 gagacgatac tgattgatcc taaaagagca cgcggtacat ttttttcagg gtcacgcatt
    24721 tccccagcga caatagagag ggtttcaaaa ccgataaagc catagaagat ataaacagca
    24781 gtattggaga tagcccctaa gaggttagtc cctggttcca actgaacaaa tggggtaaag
    24841 ttaggaagcc cgtttttgat gaagaaaagg gtacagacac aaaaggcaac gattggaatt
    24901 aacttagcaa tggtggctgt gatggtgaca attttagagg ttttcaaacc agcgatattc
    24961 atcaaagata gtaagatgat taacccaata ctaagtggaa tgtgccaacc ttcaaaggca
    25021 gggaaagtaa tgataaacat tctggcaaac ccagctgcca tggctgccca agcgaagata
    25081 gtaacggtcc aaccaaggaa gccaacgtta aagccaataa aatcaccaaa agctcgtttg
    25141 gaatattgga aggctccgcc gtttttccca aaataaccag agacttctgc aaaacaaact
    25201 gccaacatga tggtcaaaat agctgttcca aacataacag cgatggatgc agggccaagg
    25261 cccttgtaaa tggccctagg taagagaaaa ataccagagc cgataacggc attgatcccg
    25321 taaagagtgg caccgctcaa gctaaacttt gcctgttctc tttcttgttc gttcatgtga
    25381 tttgacgcca taataatgct ctcctttttg tttgcaaatt gcgttcaaat ataatggata
    25441 aggtcgctag gaaatgagga gatagtcggt ttaggagggt ccttcttgca cctaacgata
    25501 gtgatgagct attggttagc gatgagaaat aggtagcctt ttgtgatgat gtgtagtgat
    25561 ttcattggag aagaacaggc atgagtagtg tgatgcctat ctttatccca ctaagtctta
    25621 taccataagc ctgcccaaac agctactgga gtttgggcgg ggcttgtttg attggggcaa
    25681 acttttaggt ctgaaaggct ggcctcctgc tcgattacca acaagtgatt ggcttcaagc
    25741 tcttgattcg cctgattggt cacagaataa gtcagtttgc ttagggcata aagcagttga
    25801 cagtccgctt taggtgtctc aaatagtgtg tcagaccaga cgggtaataa ttgcccttgg
    25861 tattggtcat catacattaa attgatgtct tggcagattc cacgactgac gattctggta
    25921 tcgccttcaa acgcataggg ttcaaaagga gttagggaaa tctctttagc atgactcaaa
    25981 tcactcaatg tgatgggctt gtctagggtc attaggatcc tgtgatagcc atcaagtaag
    26041 gtaaaggttg tctgtcgtag ggccacagtt gactttgaga ggcgataaga gaattctctc
    26101 tttttgtaat ctccatcttc gggataaagg aacaattgat tggtttcacc gccagaccag
    26161 tcagtttgag tgtaatctgc tggcgttttg accattacgg ttgtcatctg ttctctcctt
    26221 gtttccaatt agaatagtcc tgtgatgttg ccctcctcgt caatatcaat tgtttcgctg
    26281 gcaggtactt ttggaaggcc aggcatggtc atgatagcac ccgtcagagc aacgataaat
    26341 cctgctcctg cggacacttt aaggttgcta atagtcacta taaagtcagt tggtgcgcca
    26401 agttttttgg catcgtctga gaaggaatac tgggttttgg ccatgcaaat tgggtagttg
    26461 ccaaagccga ggcgttctaa ttctgccagt tcacgtttgg ctgtgggggt caatttgata
    26521 cccttaccac cgtaaacctt ggtaacgatt ttagtcagtt tggtttcaat gctatcgtct
    26581 tcattgtaaa caaagcggaa ttgattgtct tgttctgcca atgctactac tttttcagcc
    26641 agttcacggc caccagctcc gccattcgcc caaacgtcag aaatcacaac atcaacgcca
    26701 cgtttgtcgc aggcatcata gacagcttgt aattcggcat cggtatcaag cgggaattta
    26761 ttgatcgcca cgacaactgg aagaccatag acgtcttgga tattagccaa atgtttgtca
    26821 aggttaggta aaccatccac aacggcctga acattttcag tagccaggtc tgcttttggc
    26881 acacccccat gcattttaag ggcacgaata gtggctacta ggacaacggc tgctggacga
    26941 aggcctgaca tgcggcattt gatgtcaatg aatttttcag ctccaaggtc agcaccaaat
    27001 ccagcttcgg taacggcata atcgccatat ttcaaggcta gtttggtggc taggacacta
    27061 ttacagccat gggcgatatt agcaaatggg ccaccgtgaa tgagggctgg tgtgtgttcc
    27121 agggtttgca cgagattagg atgaatcgca tccttgagca gtgctgctag ggcaccacca
    27181 gctttcaagt ctttagctgt tacaggttca ccttggaagt tgtaaccgat gataattttt
    27241 tcaaggtggg ctttgaggtc agaaatattt tcggataggc acaggattgc catgatttca
    27301 gaggcgacgg taatgtcata accatcttca cgagggaccc cgttgacctt accttgcaag
    27361 ccgtcaacaa tatgacgaag ctggcggtca ttcatatcca ccacacgttt ccaagtgata
    27421 cgtctagaat caatgcctag gctattgcca tggtgaatgt ggttatcaat caaggcagct
    27481 aataagttgt tggcgacacc gatggcatga aagtcccctg taaagtgaag gttgatgtct
    27541 tccattggga caacttgcgc gtggccaccg ccagcagctc ctcccttaac cccaaaaaca
    27601 ggaccaagag aaggttcacg aagggcaata actgcttttt ttccaatggc agaaagagca
    27661 tctaccaatc caacagaagt ggtggttttg ccttcaccag ctggtgttgg tgagatagca
    27721 gtgacaagaa tgagtttgcc atcaggtttg ttttttaagg cgaccagttg acgggcatca
    27781 atttttgcct tgtacttacc atagagacag agcgcttcct tgtcgatccc taattgatcg
    27841 gcaaccttac tgataggttc catagtgaca gagttggcta tttcaatatc tgataaaacc
    27901 atagttgctc ctttacttgt taaacaatat cgagaatttt cgtgtagatg tcatctgcca
    27961 aatgacagcc cttgtctaat aaggcttgtc ctttttggcg atagtcggta acaaagtctt
    28021 catctttgat gccagacaga ttaatcaaaa cattcaacca agcgccttgt aaacctgctt
    28081 ttaagtttaa agcagcaact cctaagtcac tagcagcatt tgtattagac ttaccgacag
    28141 ctgttgcagt gatctcaagt gcttcgacca tgagggtcat catctcaaaa ggtgattgtg
    28201 cagctgtttt caaagccttt tgcatggcag ttcgtctggc agccttgtct tcgtcagtct
    28261 cttttggcat gtcgaaaacg tcagaaacca ggttgaaggc ttcggtgtct ttgtcaatgg
    28321 catctaataa gtcctgttgc aagcgactac ttttggtatg aaggtcttca atgatatctt
    28381 gataatcagc atactttttc ttaccaaggg tcaattcaca gaccatcttg gtcaaggaaa
    28441 tgccattagc gccagagaga gcagcagcag aaccaccacc cggagcaggg gcatctgagc
    28501 caaggacttt agcgaaatct gttaagctta aatcaactaa tcccataaga caccccctag
    28561 cctagcaaat ggttttcaag aatctgttta tggtagtcaa agtcctcaac ttgcaagtag
    28621 tattcagcca catcgattaa ggctttagct ggcgctaagc ctatgatttc agaaccaata
    28681 acattcacac cgtagcgacg cgcttcaaat ttgatggttt caaaagtacg gtaaagagaa
    28741 catttttcaa agttaaccat gttcatggaa acttgggcaa tatgacggtc ttctaacatg
    28801 acaccgatag ccttacaata tttgtagcca ccacctgaac cgcggataat tttcgcaatt
    28861 ttatgagcaa tgtcgatatt gtcagtatcc aaattaacat tgaaagcaac caatggcata
    28921 cgagcaccaa cggcagttat cccagcagtt gggtgaatct tacgatctcc gtaatcaggt
    28981 gcccaatcct cttccaacag tttttctggc atgccttcga attgtccttt acggactttg
    29041 gccaggtttt gacgttctgg gcgtgtggca gaatcttcat aaaggaagat tgggattcca
    29101 agttcacggt taatccgttc tgcgacttgt ttggaaatct caacacattc ttgtgtagtg
    29161 atgtctttga tcggaacgaa tgggcagaca tcggttgctc ccatacgagg gtgttcacca
    29221 tgatgtttgg tcatatcaat gttttcagaa gcgtatttca ccagttggaa agctgcttct
    29281 tgaatagact ggtcatctcc aaccaaggta aacacgctcc ggttgtggct cgcatcagaa
    29341 gagtagtcga gaagggtcac tccaggaata cttttggccg tcgctaccaa accatcgata
    29401 acagcctgat tttggccttc tgagaagttg ggaatacatt caacaatttt cgccatgtta
    29461 atctcctttt atgtcattga gttactcatt aagggaatag gttacctact ttagataacg
    29521 cttacatttt tagctagcag ttattagtga aataattgat ctaccgctga agtcacaagg
    29581 tcatcatcag ccaagtatgg gatagtgatg tgatcagtac ctgcatggag acggttgtat
    29641 tcaatagcgg tttcaatagc gtgctcattg cgagcccaat tgcgacgagc aacaccaccc
    29701 atggtgtccc aagcaatagc agatttaata atctcatcaa tgcgttcgct gccatctaga
    29761 acaagtccaa agccgccgtt aatggcttta ccgataccag ttccgccacc gttatgaaga
    29821 gccactaaac tcataccacg cgcagcgtta ccagcgtaac attgaactgc catatcacag
    29881 gtgacattgg aaccgtcttt aatgtttgag gtttcacgga atggtgagtc agtaccagac
    29941 acgtcatggt ggtcacggcc aatcatgaca ggtccaatct taccttctcg gaccaattca
    30001 ttaaatttaa gggcgatagt cacacgacca atacagtctt ggtaaaggat acgggcttgt
    30061 gtgccgacga ccaattgatt tttctcagca tcacgaatcc agttatagtt atcacggtct
    30121 tgatagcggc ggtcaggatc gatagcttcc atggcagctt tgtcagtagc caccaagtct
    30181 tcgtgtttac cactcaagca gacccaacgg aaaggtccat agccatagtc aaaaagcata
    30241 ggtcccatga tatcttccac ataggatggc cagataaagc cgtctttgtc gttacgacca
    30301 ttcttagaaa tttctgtaat accagagtcg taaacagact tcataaaggc attgccgtag
    30361 tcaaagaaat aggtgccatt ttcagttaac gtcttaatag cttcaaaatg ccgtgccaag
    30421 gtatcatcaa ccatttggtg gaaggtatcc ttgtcttctg ccaggagccg ggttctttcg
    30481 tcaaagctaa tacctactgg gcagtagcca ccgtcataaa cgttgtgaca agaggtttgg
    30541 tcagacaaaa gatcaacatg aatctgcttg tcattgacgt actctaaaag gtcaacaata
    30601 ttaccatggt aagcaatcga agtggactcc ttagcatcta tggcttttcg ggctaattgc
    30661 agagcttctt cagggctctc agcaatttgg ctaacccagc cttgcgaatg acgggtttca
    30721 atccgagatt ggtcaacttc agcaataatg gcaactgatt tagcaatttc agcagcctta
    30781 ccttgtgccc cactcatgcc tcctaaacca gatgagataa agagtttacc ggtcaaatca
    30841 ccatcgtcag ccactccaag tttgagacga ccagcattta agagagtatt aaatgttcca
    30901 tgaacaatcc cttgtgggcc aatatacatc caaccacctg cggtcatttg accatagttg
    30961 gtgacaccca tctcttcggc gatttcccag tctttcatgt tgtcgtattc acccaccaaa
    31021 aggccattag taataatcac acgaggcgct tcaggtttgg atttgaaaag accaactgga
    31081 tgaccagact caaccaccaa ggtttgttca tcagtcatga cctctaggta ttttttaatc
    31141 aaacaatatt gcatccagtt ggcacaaaca gaaccagttt ctccataagt gactagttcg
    31201 taagggtaca aggcaatttc aaaactcaag ttattgtcaa tcatgacctg catggccttg
    31261 gcagctgtac aatttccctt gtactcgtca atgggtttgc cgtaaatgcg gtcttttgga
    31321 cgccatctgt aaccatagat gcgtcccctt gttttgagtt cttccaaaaa ttctggaatg
    31381 acttcttcgt ggaacttcgt aggtacatag cgtagggcat tttttaaggc aatttcggtc
    31441 tgagcctggg tcaatcgaaa acctctgtct ggtgatcttc ggataccttc tttgaacaca
    31501 gtcttttccg gtaaaacatc gtctaactta acagtcatag cggcagctat gtcagtttca
    31561 ctgtaaaatg acatagaaaa ctcctcctca actccttttt attgtcgaga ttagtctaac
    31621 ttagtctagt ctataaaaag tcaaccacgc tttgaaatgc gaaaaaagtc taaaaaaaaa
    31681 tcaaaacctc attttttgac taaaatttga cgcttaatca gtacacttga ggtatgacag
    31741 ataaagtcag tcagaggtta attgttgtta ccaaagttgc agagatcgca actattgagt
    31801 gggtgagggg ggctagtaat ggctattaat cagttgacaa acgatgttaa aaaaggaggt
    31861 ttttatggtt gctgatgtct tattaacaca ttttaatcaa ctattttgtc taaatgatcc
    31921 aggtcatcct ttaactggtc aagaaatgaa aaaagccact atcgttgaag atggctatat
    31981 agccatcaaa gacggcctca ttgtggctct tggttctggt gaaccagatg ctgagcttgt
    32041 cggtccacag actattatgc gttcttataa aggtaaaatt gcaacgcctg gtattattga
    32101 ctgtcatacc catttggtct atggtggcag tcgtgaacat gaatttgcca agaaattagc
    32161 aggggtgtct tacttggata ttttagctca aggtggtggt attttaagca cggtgagggc
    32221 cactcggtca gccagctttg acaaccttta tcaaaaatcc aagagattgc tagactacat
    32281 gttgcttcat ggagtgacta ctgtcgaagc gaaaagtggc tacggccttg attgggaaac
    32341 agaaaaacgc caactggatg ttgttgctgc tttagaaaaa gaccatccta ttgatttggt
    32401 gtctacgttt atggcagccc atgcgattcc tgaagaatac aaggggaatc ctaaggctta
    32461 tctcgatgtg attatcaagg atatgcttcc tgttgttaaa gaggaaaatc tggcagaatt
    32521 ttgtgatatt ttctgtgaaa aaaatgtttt tacagctgat gagtcccgct atctgttaag
    32581 caaagccaaa gaaatgggct tcaagcttcg tattcacgca gatgaaattg cgtctatagg
    32641 cggagttgat gtcgcagcag agctcagcgc agttagtgca gagcatttga tgatgataac
    32701 aaatgatgat atcgctaagt tgattggtgc tggtgttatt ggtaatttgc ttccagccac
    32761 cacctttagt ttgatggaag acacttatgc ccctgctcgc aaaatgatcg atgctggcat
    32821 ggccatcacc ctctcaacag atagtaatcc aggcagttgc ccgactgcta acatgcagtt
    32881 tgtgatgcaa ttaggttgtt ttatgttacg tttaacaccg attgaagtct taaatgctgt
    32941 taccattaac gcggcctact ctgtcaatcg tcaggagaga gttggtagtt tgacagttgg
    33001 taaagaagca gatattgcca tttttgatgc cccaaatatt gattacccat tttatttctt
    33061 tgccaccaac ttgattcacc aagtctataa aaaaggacaa ctcacggttg atcgaggtcg
    33121 tattctttaa aaaatttcaa aaagaagctt tgagcgttag ctcaaaactt cttttaaata
    33181 tgctatttta ttgacgaatc aagtagtcaa aggcaccgat ggcagcagta gcaccagacc
    33241 ccatggaaat aatgatttgt ttataagctg aatcagtaca gtccccagca gcaaagatgc
    33301 cgggaatatt ggttgaacca tgtttatcca caatgatttc tccgcggtcg gttaggttga
    33361 caccactatc tttgagccaa gctgtgtttg gaacaagacc aatttgaaca aagacccctt
    33421 caaggtcaag gtgtttgtct tcaccgctat cacgttcagt gtagttgaga ccagtaacat
    33481 gatcgtcacc aacaatgtct ttagtagcaa catttttgat aatagtcatg ttagcagtat
    33541 cagctgcgcg gtcttgaagc actttgtcag ctttgagctc aggcaagaat tctaacacgt
    33601 aaacgtgttt agcaagacca gccaagtcaa gggccgcttc taaaccggag ttaccaccac
    33661 cgataacagc aacatctttg ccttcaaaga gtgggccatc acaatgagga cagtaagtca
    33721 cccctttatt acggaattca tcttcaccag gaacattaat gttacgccat ttggccccaa
    33781 gagctaagat agcagttttg gcttgtaaaa ccgcaccatt agctagagta acctcgatgt
    33841 tttctttttt ctcaatagaa gtagctagtt gtgctttgat aatatcaaca tcataagact
    33901 tagtatgggc ctccacttcg gccattaatt ttggtccttc agtgtaaagg gtaccaatca
    33961 tgttttcgat gccgaccgtt tccatgacct gaccaccaaa ggtttcagca agtaaacctg
    34021 tttttagtcc tttacgagct gcatagatag cagcgctatt gcccgcaggg ccaccaccaa
    34081 taacaaggac atcatagagg cctttatcag caaaggcttc ctcagaaagt gggccagcga
    34141 tttgttctaa gagttgctca atggtagcac gaccagaagt gaattcttcc ccgtctaaaa
    34201 agacagtagg gacagacata atgcctttgg ctttcacttc atcttggaac atgccgcctt
    34261 ctaccatagt gtgtgaaatc ttgtcgttca agacggacat aatgtttaaa gcttgaacaa
    34321 catctgggca gttatggcag gttaagctga cataagtttc aaaatgcaac ggacggtcaa
    34381 tggccttgat gcgatcaata acatcttgat ccacttttgg agcacgtcca gatacttgca
    34441 agagagcaag aataaaggac gtcaattcgt ggcctaaagg aagaccagca aagacaaccc
    34501 cactatcgtg accttttttc gcaaccttaa agcttggttg tcggtctaaa gtgatgtttt
    34561 caatagaaat acgctcagac atagcagcta tttcttccac aaagtctttg actttttgag
    34621 actgctcatt gtcaccaagg gaaacttgca aaacaagatc agcttctaac agggtgaggt
    34681 attgggcaag ttgttcttta atatcaggac ttaatgccat agctttcctc ctaaaattta
    34741 ttaaatttta ccaactaaat caagacttgg tgtcaatgtt tcagcgcctt ctttccattt
    34801 agctggacaa acttcacctg gatgtttacg gacgtattgg gcagcgtgaa ttttatcaat
    34861 caaggtgcta gcgtcacgtc caataccatc agcattaatt tccatcattt ggataatacc
    34921 atctgggtca acgataaatg ttccacgttg agcaagtccg tcttcgccaa gcacttcaaa
    34981 ggcttgtgaa ataaggtgtg aagggtcacc aatcataggg tatgtgatag tgccaaccac
    35041 atctgaatca tcatgccaag ctttatgaac aaaatgagta tcagtagaga cagaataaac
    35101 ttctacacca agagatttca gtgtttcgta ttgctcttga aggtcaccga gttcagttgg
    35161 gcaaacaaat gaaaagtctg ctgggtagaa acaaaaaact gcccattttc ctttaacgtc
    35221 ttcatttgta acagtgatga attttccatc gtgataagct tgagctgaaa attcagcaat
    35281 ttcttttcca attagagaca tatagttgtc ctcctttttt atagttttat acttgtatta
    35341 taaaagaaca acaagattat gtctactcag aaacccttga ttttactggg aaatcagcga
    35401 ttgttttctg acaattcagt tgtgaaataa atcacattaa ttaaaatgat tctaaataag
    35461 ctgaagacgc cgttaaatca aaggttaatt taattataat ctacagaaaa tataattgtt
    35521 attaaagcgt ttacaaaaaa cttttttgat tccgttaagg caacttaata ttatggttac
    35581 ctaatcacta atattgtaac ctattttggc atgagatcag ggaatggctg ttatattaat
    35641 tggatatgtc ttgtgcaaat agtgcttgac ggatttgtgg aattagggta aaataatctt
    35701 tgttgtggcg gtatagccaa gtggtaaggc acggctctgc aaaagcttga tcgtcggttc
    35761 aaatccgtct accgcctttt atatacaaaa caagccttga ttaacttcgt gaaaacgttg
    35821 ttaaatcaag gtttttattt tttgcgacag gataaaaaaa gagaaaaaaa gaaataaagt
    35881 cggtcaaaag tcggtcaaat ttcccgaaga tgtcttttat agcctgatta ttaggtatga
    35941 aaaatactat ggccaaaatt ggtaaacgat tgcaaatgaa ggtcagagtt cttaccccct
    36001 taaacgatat gcgttaaaag ttagttgaac tgccgtgcac ctaacgatag acacggtgat
    36061 gtgagagcga gtagagttga atctctactc atttatctta ccatagtcag ctttataaag
    36121 taaccaccca ttacaatgca actatattct taatgttttt aagcatgtta ttgacaagtg
    36181 ttataaaatg tgttactctt agtgagtaaa ttagtttttg agttagtatt tgtgtttcaa
    36241 catctttatt tctctctatt tttacaaatg atttttagaa aggaagtgga tgtatatggc
    36301 acaaggtaca gtaaaatggt ttaacgctga aaaaggtttt ggttttattt caactgaaaa
    36361 cggtcaagat gtctttgcac atttttcagc aatccaaact aatggtttta aaacgttaga
    36421 agaaggacaa aaagtggcat ttgacgtcga agaaggtcaa cgtggacctc aagcagtcaa
    36481 tatcactaaa ttggcctaat ctggttaaag taactaagga gaagcagatg ttggaaacaa
    36541 caactgcttt tttgatttct accagttacc tttaatgatt aagagttgcg tttaaggtct
    36601 tgtatttgtt ataataatag tcagttttag tcaaaatgta ttgacgtggt gctactaagt
    36661 ttttagagta gcccgcaagc aaaggagtga cagcatgcca accaaaaata cttcagacag
    36721 cattgaagag tatattaaag aattattagc taagtcagga atagcagaaa ttaagcggtc
    36781 catgctagca gattcgtttc aagttgtccc aagtcagatc aactatgtca ttaaaacccg
    36841 ttttacggag agtcgaggct atgaagtaga gagtaaacga ggtggtggag gctatattcg
    36901 tattgccaag gttcattttt cagacaaaca ccatctgatt ggcaatttaa tggcaactat
    36961 tgaagactgt atcagtgagc aagttttcac ggactcgata caattgcttt ttgacgaaca
    37021 cttattgaca gagcgtgagg gaaatatcat tttggctatg gcgtctgatg atgttttagg
    37081 aactgatggt tcaactattc gtgcacgaat gctctatcgc ttattgcaac gaattgacag
    37141 aaagggaagc aattaatgat tatgtattca acgaagatgc aagacatttt tagacaggcg
    37201 cagttccaag ctgctcgctt tgatagccat tgcctggaaa cttggcatgt tttgttagct
    37261 atggtagctg tagataattc tttagcaaat atgattttaa gtgaatatga tgcccaagtc
    37321 gccatagaag aatatgaagc tgcagctatt ttagccatgg gcaaaacccc taaggaacag
    37381 ttgtctcgtg tagacttcag acctcaatct aaaaccttga ctaacttgtt agcttttgcg
    37441 caggctatta gccaaatcac tagggatcaa gaagtcggct ctgagcatgt cttatttgct
    37501 attttattga atccagatat catggcgagt cgtttgttag aaatagcagg ctatcagata
    37561 aaagataacg gcaatgggca gccgcgatta gctgacttgc gaaaagcaat agaacgtcat
    37621 gcaggttaca gtaaggaaat gattaaggct attcacgaac tacgtaagcc taaaaaaacg
    37681 aaaacacaag ggaccttttc agatatgatg aagccaccaa gtacagctgg tgagttgagt
    37741 gatttcacaa gagatttgac tgaaatggca agacaaggtt tgttagaatc ggtgattgga
    37801 cgtgaccaag aagtatctcg tatgattcag gtactaagtc gtaaaacgaa aaacaatcct
    37861 gtcttggtag gtgatgcagg tgttggtaaa actgcgcttg cttatggcct tgctcaacgg
    37921 attgcaaatg gcgctattcc ttatgaactt aaggagatgc gtgtcctaga attagacatg
    37981 atgagtgtgg tagcaggaac ccgttttcgt ggggattttg aagagcgcat gaatcaaatc
    38041 attgatgata ttgaagctga tggtcagatt attctttttg ttgatgaact acatactatt
    38101 atgggatctg gcagtggtat tgacagtaca cttgatgcgg ctaacatttt aaaaccagca
    38161 ttatcgcgcg gcactcttca tatggttgga gcaacaactc aagaagaata tcaaaaacat
    38221 attgaaaaag atgcagctct ttcgcgtcgt tttgctaaaa tattaattga agaacctaat
    38281 acagaagatg cttatcagat tttgatgggc ctaaaattat cttatgagac ctaccataat
    38341 gtctcgatat caaatgaggc agttaaaaca gctgtaaaaa tggcacaccg ttatttaacc
    38401 agtaaaaatc tccctgattc agctatcgat ttattagatg aagctagtgc tgctgtgcaa
    38461 aacatggtga aaaaatcagc acctgagact ttaacaccaa tagaccaagc tcttatcaat
    38521 ggtgatatga aaaaagtatc tcgcctctta gctaaagaag caaaaggtca gatgagaaaa
    38581 ccaacaccag tcacagaaga tgatattttg gcaaccttga gtaagttatc gggaattcca
    38641 cttgaaaaac tgacgcaagc tgatagcaaa aaatacctca atttagaaaa agaactgcat
    38701 aagcgtgtga ttggtcagga tgctgctgtt acggctattt caagagccat tcgtcgcaat
    38761 cagtcaggta ttcgaacagg aaaacgtcct attggatcat ttatgtttct tggcccaaca
    38821 ggagtaggta agacagaact agcaaaggcc cttgcagaag ttctctttga tgatgaagca
    38881 gcgcttattc gttttgatat gtctgagtac atggaaaaat tcgcagcgtc taggcttaat
    38941 ggagcacctc ctggttatgt cggctatgat gaaggaggtg aactgacaca gaaagttaga
    39001 aataaacctt attcagtctt gctttttgat gaagtggaaa aagcacatcc tgatattttt
    39061 aacgttctcc ttcaagtatt agatgatggt atattgactg atagtcgtgg gcgtaaggtc
    39121 gatttttcaa atactattat tatcatgacc agcaatcttg gcgcaacagc cctgcgtgat
    39181 gataaaacgg tcggttttgg ggtcaaaggc attcaccaag accatcaagc tatggagaaa
    39241 cgtatcttag aagaattaag aaaaacttac cgcccagaat ttatcaatcg tattgatgaa
    39301 aaagtggtct ttcatagtct gacccaagat aacatgcgcg atgtggttaa aatcatggta
    39361 cagcccctga ttactacatt ggcagaaaaa ggtattaccc ttaaaattca gcctttggcc
    39421 ttgaaacatt tgtccgaggt cggctatgat gaacatatgg gggcaagacc attacgtcga
    39481 acgctgcaaa ctgagataga agataagcta tcagagctta tcctttctcg agaattgaca
    39541 agtgggcata cgctaaaaat tggattatca catggcaaat taacgtttca catagcttaa
    39601 tttccatata aaaggtggat catccttgat gtcaccaaaa aaagaggtta ttcttacaaa
    39661 aataacttct tttttcttga ctatttttga ccaagtgata aaatagaata taaagttagc
    39721 actcgatgaa catgagtgct aactttacta aagattcttt tattggagga aaatttcatg
    39781 ttaaaaccat taggtgaccg tgtggtcgta agatttgatg atgaaaaaga gcagacagtc
    39841 ggtggttttg tccttgcagg cactcataaa gagtcaacac gaaaagcgac agtcctcgct
    39901 gtcagtgaaa cgggtgttcg cactattaca ggcgatagcg tgctaccttc agtttcagtg
    39961 ggtcaagagg ttttagttga aaatgggcat gatttagaag taaccgtaga tgatgagaaa
    40021 gtttctatca tccgtgaatc tgacatcatt gctattgtca caaaataaaa cgacaaatct
    40081 ctaaatagaa agaaggattg aatatggcaa aagatattaa attttcagca gatgcgcgtg
    40141 ctgccatggt gcgcggagtt gatatgttag cagataccgt caaagtaacg cttggtccta
    40201 aaggacgcaa tgttgttctt gaaaaagctt ttggttctcc cttaattact aatgacgggg
    40261 taaccattgc taaagagatc gaattagaag atcattttga aaacatggga gcaaaattgg
    40321 tgtctgaagt ggcttctaaa accaatgata ttgctggtga tgggacgact actgcaacag
    40381 ttttgacaca agccattgtt catgaaggac taaaaaatgt gacagcaggt gctaatccaa
    40441 ttggtatccg tcgaggcatt gaaacagcaa cagcaacagc tgttgaagcc ttgaaagcca
    40501 ttgctcaacc tgtatctggc aaggaagcta ttgctcaggt cgctgcagta tcatcacgct
    40561 ctgaaaaagt tggagagtat atctcagaag ctatggagcg tgtgggcaac gatggtgtga
    40621 ttaccatcga agaatctcga ggtatggaaa cagaacttga agtggttgaa ggcatgcaat
    40681 ttgaccgtgg ttacctgtct caatacatgg tcacagacaa tgaaaaaatg gttgcagacc
    40741 ttgaaaaccc atttatctta atcacggata aaaaagtgtc aaacatccaa gatattttgc
    40801 cactacttga ggaagttctt aaaaccaacc gtccattact cattattgca gatgatgtgg
    40861 atggtgaagc ccttccaacc cttgtcttga acaagattcg tggtactttc aatgtggttg
    40921 ctgtcaaagc gccaggattt ggtgatcgcc gtaaagctat gcttgaagac attgctatct
    40981 tgacaggtgg tacagtgatt acagaggatc taggacttga attaaaagat gctacaatga
    41041 cagcccttgg acaggctgct aagattacag ttgataaaga tagcacagta attgttgaag
    41101 gttcaggaag ttcagaagct attgctaacc gtattgcact gattaaatcg caattagaaa
    41161 caacaacttc tgactttgac cgtgaaaaac tacaagaacg tttggcgaaa ttagctggtg
    41221 gtgtagctgt tatcaaagta ggagctccaa cagagacagc tttaaaagaa atgaaacttc
    41281 gcattgagga cgctctaaat gctacacgtg cagccgttga agaaggtatc gttgctggtg
    41341 gtggaacagc acttattacg gttattgaaa aagtagcagc tcttgaactt gagggcgatg
    41401 atgctactgg acgtaacatt gtgcttcgtg ctctagaaga gcctgtacgt caaattgctt
    41461 taaatgctgg gtacgaaggc tccgtagtta ttgacaagtt gaaaaacagc cctgcaggaa
    41521 caggatttaa tgctgcaaca ggtgagtggg ttgatatgat taaaacagga atcattgacc
    41581 ctgtcaaagt aacacgatca gcgcttcaaa atgcagcttc tgtagctagt cttattttga
    41641 caacagaagc agttgtcgct aataaacctg aaccagctgc gccagcgcca gcaatgccag
    41701 caggtatgga tccaggaatg atgggcggct tctaaggtct ctatagcatt aaggattatg
    41761 aaaaagaagt tgagactaaa ggtctgcttc ttactatgaa aaaagaacaa agattggatt
    41821 tcgactcatg atatcccatc tttgtttttt tagttgtgat ataaaatgct aatctttgtg
    41881 gttacagact tttaatgtat tggaaaatac attgtttttc tttgtcatgt gctatcatgg
    41941 agtcaatgaa aagttattcc catatatggg tgttggaggt ctgtgatgaa tcattttgga
    42001 gagattttaa aaacctttag agaatcaaag ggattacgat taaaagatgt cgcaaaggct
    42061 ggtatatcaa cctctcagct gtcgcgtttc gaaaaagggg agacggactt aaccatatcg
    42121 acatttatgc tgattttgga tgaaagtaac atgcccattg atgagtttat gtatgctgtc
    42181 catgattttc atcgtgacga tttaaatgaa ctcttatcaa aaagtgaggc atttcgtaac
    42241 aactcaagat agtgatggct tgaagcagtt acttcatgct caactagcat aaactgataa
    42301 gaaggagatg ttcacggata tcttttggca tatccagcat gtctaagtct gctatggcat
    42361 gctattttgg gagtccctca cgcgactctg tcagtagctc tccttcaaag gattcacata
    42421 gaaagtcgta atagacatag cagtcaggct tgataaagct agaaatactc tttaattgaa
    42481 ggtttaaagc tgtcagacct atctcttctt ttagttctca taaagccgct tcaaagaaag
    42541 attcacaggg ctcgacttta ccttctggct ggacccatcc tgggaagtta tcgtgttgtc
    42601 tatggagtca taaaatcata tcgttatgtt tgacacacat attgccccag tctctaaccg
    42661 ttgttatcac cagattcacc accttttagt atagtataag aaaaaagggg taaaagaggt
    42721 ttcttgttgc ttttttggct taaggtttct ttaagtctta tctagtatac taatgtcatt
    42781 ccttaatagg ataagtattt ggttgtagaa gtatcgtctt aaactaacgc tatctcaacc
    42841 cgattcccat cttattcacc cctcctatac cgataaggtc tcacaaagtg tgagacctta
    42901 ttttttgact tcagctctag ttatggagaa acttgttgat acaagctttt ttgggttatg
    42961 gtattatgat ctattcaagc ctttcttgtt tttgggtttt tctttaacca acttggcttc
    43021 aatagctttt aagcgtttga aggttttttc agcgcgatct agtgaaatct tatcagcttt
    43081 ttcttgagca gctttggggt cactgacgac taagtcactg atttctttgg tgcttttgtc
    43141 ttgttcggct atccaggttt tttccaagcc tttcatctca tcaatcactt tagttccaaa
    43201 tggttttgga tgagcagcca cgagatcatt aatgtgtgac accgtccaat accatgactt
    43261 atcgttatat tgggtgcttt tttcttgata ggcttcatag gttctactga tattgccaag
    43321 gtatggcaag taaggggcgt tacgaggact gccaatagat agccacatga caccaccacc
    43381 caattcagca gggatgtctt ttttcagttg gaaaatatga gcttccatga cattaggatt
    43441 tgagatggga taagcataac ctttaacagc ttttttggat tttggtttac ccttaccgtc
    43501 taatgccatc tgatcaagtg gtttaagatc cagcccctca aaacgattac gttgcagttt
    43561 catcgcatct tctagactga aggtcttatc agtgctttgg agcaactcat aattgctgtc
    43621 tttataagtt acttttgaat cagggtcaag tgatttaatc cctgagaaag agcgagagcg
    43681 gttggcatcg tttagtggcg gattgtaaga tttggcaata tgaaatttac catcaacttc
    43741 gatataagat ttggcttttt tagcgacttt ttccacatct tctgaggcaa tcgtattttc
    43801 cttatcgttg aaatcgacat gtccgagata gaaggtattt ggaaaaacag cgtatttatc
    43861 atctgggaat ttgatggcaa cgtattggtg accagagaga atttccatgt accagatgcc
    43921 gtctttgtca gccaaggtaa caatatttcc ttcagcagct cctttttcag tgactatttt
    43981 agcaatcaat gcgacacctt cacgggctgt tttgacgctt ggtaaaatga cactggccat
    44041 agatgattcc gctaagccat ttttgacgta aggatcaatt ttttggatgg catcattagc
    44101 tgaagctgat actgttgcag acatagagac gccaaactca ttaaaaccag cttcgtcata
    44161 aacgcctttg ttaggtgtta catcaggaat cgcactatag cgataagaat gctcaggcaa
    44221 cgggtactca aaaccattag agaggtcttt ccatttttcg ccagcaggat tgtcctttgc
    44281 tagacgtaca atgaaatttt tattatgatg tggctcaaga tcttctgttc gaccataaag
    44341 aagagaaccg tctttggtta aatctttgcc aataataaaa ccagtacaag cataactaac
    44401 actttgaagc gaaaaggctg tcagaatact taaaactcca agtgatattt ttttgtttat
    44461 catgcgaatc ctcctagtaa acgataacgc tttcacagac attataaaat gctcttttta
    44521 aaaattcaag tcttttatga agtgttttga aattgttagc tatctaaata ttatgagaat
    44581 tctctcttga attttggctt aacggcccct aaaaacgtat aggcagtgaa ctatctaggt
    44641 ttcttcgaaa atccattagc catttatgat ataatagtaa agaaaaacat agaaagttgg
    44701 ggtctgttga tgaccaaacc atttcatcat aagaaactaa aacaaataac tataatagct
    44761 gcaactagcc tttttttatt cctgatcggt ggtgcctttt actactctaa aaatcattgt
    44821 attaatgcct atcttaaagc tcgatcagcc caatcaggac cagtttttga gaatatcaaa
    44881 gcatatctag tctgggatga tactaatgag cagatcacaa atgacgaggc gatgtatact
    44941 aagtttagaa ggtatagtca aaaagaattg aggcaaaaaa agcaggattt aaaagctgct
    45001 agtcaagata gtgccgttca agtaaagtct gttggtcgtc gtttttggat ttttcctgat
    45061 tatcggattg ccataaaacc catggattta acgataaaaa cgaatgtgcc tcaagcagac
    45121 gttcttttaa atcaaaaaaa agttgctgtt tctgattcag aacagttctc agtcaagctt
    45181 gatcggctac caacggcaga atataccgca agtatcagag gcaaacacaa cgggcgaaac
    45241 attaaagtca ataaatcata tgatggtgat aatcccgtgc tagatttgag tgtgtctttt
    45301 agaacttttt tagtaacaag caatgctaag caaggagatc tttacttcga tgataaccat
    45361 attggcacat taaaagatgg tcaattacaa gtagaagatt accctgttac agaaaacgca
    45421 caagcttata tgaaaacaac attcccagat ggtgagttaa gatcacaaaa atatgctcta
    45481 gctgatgttg aagaaggagc aaccctggag attttagtta cagatctttt agaagaggac
    45541 aaagcagggg agctattagt atcagctttt gatcagctga tgcactacct cagtacaggt
    45601 caagactcat ctaacttacg tagcgtcttt gaggcagggt ctagcaacgc attttatagg
    45661 ggattgaaag agtccataaa agcgaaattt cagacagata caagaaaagc cagccgtctc
    45721 aatattccat ctatcctttt gacaacaatg actcaagtgg gcaaaacaat ttacgtgctt
    45781 gatttcacag ctacctacga atttttgtat gacaactcaa cagatcctga gcagcatacc
    45841 tctggacata ttaatcaaga cttgactgga aaagtgactg taaaaaaagt tggacagcat
    45901 taccttatca gccagtccgg ctccaaaaat attactgttg ttaaagagga caatcaactc
    45961 aaagcgccat ctgtctttcc tgagtctatt ttgggaacgt ggacaggcca agccaatggt
    46021 ttgagcatcc atatgtctct agcatcagat ggaacaatta cgactaaagt tgaagatcaa
    46081 aaaggcaacc gttctaaaga aactcggaca gctaaaatta gtaaagttga agacaaaggc
    46141 aatggttttt atctttatac gccagatcct ggaagtgaca taagcgcctt agctccagaa
    46201 ggaggattgg ggggtgcaaa tgtcaaatat gcttatggtt tcaaaatatc tggtaaaaca
    46261 gcctctccag tggtgtggca ggcagcatta acacatgaat ttgattatac caaaccactt
    46321 tcgggagtaa ctttgcaaaa gcaaccataa aggatgcaaa gccacaaaga acagtagaaa
    46381 caatgttatt actcaaatac agtgctatga aaacagataa actcaaaagg ctaggagatt
    46441 atttcctagc cttttgagcg tttaagactg cgtcaaatgt agctgattta gcttgcacgc
    46501 aaatgtcttc tcgggttaga gggtgtttca aacgtaattg ataagcatgg agcatcagtc
    46561 gagcacaatc tttttttcca ttagaataca aaggatcacc aaataaaaca tgaccttgat
    46621 gggctaaatg gactcgaatt tgatgagtcc gtccagtttg taattgacaa ttgagtaagc
    46681 ttgccgtttt gtggaagttt ttgactagag ttacctcagt aatagctttc ttgccattta
    46741 tcggatcgac aacacgtttt ctgcggtcat gtcgatgacg accaattgga tgatggtaag
    46801 ttaccctagg actgtctaag gatccgtgaa ctagtgccaa atattctcga tggatgtctc
    46861 ttttttctaa aagacgatta aggattggta agataaaagg tgttttagca aataaaatgg
    46921 ccccacttgt ttccttatcc agacgatgaa ccacatagca ggtctgtccc gtgtaagcag
    46981 acacgtggtt gagcagcgcc aactctgttg gatcattgcc gtgtgttttc atcccttcag
    47041 gtttgttaac aataatgatg tgctcatctt cataaaggca ggttactttt tcggcctgcc
    47101 ccatcacgat aatcttttca ggataatctt cgtgatcgaa aaaaagtttg acctgatcac
    47161 cgtatttaac acaactttgc cagttaacag gatgaccatt aataagaaca tgttttttag
    47221 ttctcagaaa atgcctaatt ttacggggaa ttaacaattg ttcctctaat aatgctttga
    47281 ccgtcaattc ttcaaaggga tttttcagtg tgacagtaaa cccctttgat ggtttatttg
    47341 ttgtcttcat tgagccagtt atggtatttt tcaacaaggt tgatagccat ttgggtggca
    47401 agagcagcag agaaagcttc agtaccttca cgctcgtcat tagctacctc caagcgagtc
    47461 tcatcgtaaa tggcttttag actttctgcg gataaggttt ctttaaaagc ttggaattta
    47521 ttcattggtt aaacccgtcc ttctatgttt aatgtgagct cctgattaat gcaaggaatt
    47581 caatgctatt atagcatgtt tctaatcaaa acgtgccact caaaaaggta aaaacgttat
    47641 tttgctaatc catctaataa gaaagtgtct taaataagac ctcttgatag agtttgtaac
    47701 aaaaatgata tgaaactagg tgctaacgca gaaatagaaa ccaactaacg tgttgcgaag
    47761 ttggaacagt cataaaaaat agcaagataa aacttatcaa cacacagtta cctaagacga
    47821 tgagtcaaac aggtatgttc tttcaaaaca accccatgag gttgaacgac gtatcggaaa
    47881 aatacggatt agtttggcct tatgataggt atttctctat cgtaaaagaa gtcaaccaaa
    47941 ggtttttaat ataaaatatt aagaaaaatt gttataatta tgataaaaac ataatcgtaa
    48001 aatagaggaa aaacaatgaa aacaattaga cgttatgatg ttaacgaaga tagagggcat
    48061 acaggccttg tagaagcagg agacttctat tatttaaact attgtgtcgg caatgtcgga
    48121 caagacattg aaagtcagat taatggagcc tttgacgaga tggagcgccg attagcattg
    48181 gtggggctca cattagatgc agttgttcaa atggattgtc tatttcgaga cgtttggaac
    48241 atacctgtga tggaaaaaat gattaaagag cgctttaatg gcagatatcc tgctcgaaaa
    48301 tcgattcaaa cggaatttgc acatcacggt ggaccacaag gactactctt tcaagtggat
    48361 ggtgtggctt attcaaaaca tatttcgatg acctaatcaa ggttacttta agatcatagt
    48421 ggtataataa cgttatgaga tttctagaac ttttacaaaa gaaatttttt cctaaagcat
    48481 atcaggaaaa acaattctta atgcatcaaa aaacgcgttt aacgccacaa cacaatcaaa
    48541 agcagtattc gccaaatgcc aatcatttgg actcatcagc taccaaaaac tcagaacaag
    48601 accctgcaat agctctgcaa cgcagtagag cctatgaagg aagccctaaa agtcggcccg
    48661 cttggttgca aaagctggaa gctgttttgc cgtctcctca acatccaatt cggcgttttt
    48721 ggcgccgcta tcacatcgga aaactgctaa tgattctgat tggaactctt gtcttactct
    48781 taggatcata cttgttttac ttatcaaaaa cagctaaagt atctgattta caagatgcct
    48841 tgaaggctac aactgttatt tatgatcaca aaggagagta tgcaggcagt ttatctggtc
    48901 aaaaagggag ttatgttgag ctcaacgcta tttcagatga tcttgagaat gctgttattg
    48961 ccactgagga taggactttt tacagtaata gcggtattaa tcttaaacgc ttcttattgg
    49021 cggtagttac ggcgggccgc tttggaggtg gctcaacgat tacacagcaa ctggctaaaa
    49081 atgcttatct ctcacaagat cagacaatta aacgaaaggc cagagagttt tttttggcgt
    49141 tagagttgac caaaaaatac agtaaaaaag atattcttac tatgtacctt aacaactcct
    49201 attttggaaa tggagtttgg ggagttgaag atgccagtca aaaatatttt ggaaccacag
    49261 ctgctaactt aacattggat gaagctgcca cattagcagg tatgctcaaa ggacctgaaa
    49321 tatataaccc ttaccattct ctaaaaaatg ctactcaccg tagagatact gttttaggag
    49381 cgatggttga tgccaaaaag attacccaaa caaaagctca gcaagctaga gcagtagggc
    49441 taaaaaatcg cttagctgat acttatgttg gtaagacaga tgactacaaa tacccatcct
    49501 actttgatgc tgttattagt gaagcaatag caacttatgg tctttcagaa aaagacattg
    49561 ttaataatgg atacaaagtt tacaccgagc tagatcaaaa ttaccaaact ggcatgcaga
    49621 cgacttttaa caacgatgaa ctatttcctg tttcagctta tgacggtagc tctgctcaag
    49681 cagctagtgt tgctttagat cctaaaacag gaggtgttag aggtctgatt ggtcgtgtga
    49741 atagtagtga aaatccgact ttcagaagtt ttaactatgc gactcaagca aaacgtagtc
    49801 ccgcatcaac aatcaaacca ctcgtggttt acgcgccagc cgttgcttca ggatggtcaa
    49861 ttgaaaaaga actaccaaat accgttcaag atttcgatgg ctatcagcca cataattatg
    49921 gaaattatga atcagaagat gttcctatgt atcaagcatt agcaaactct tataatattc
    49981 cagcagtttc tacattgaac gatatcggaa tcgataaagc ctttacctat ggtaaaacat
    50041 ttgggttaga tatgagctct gccaaaaaag agttgggggt agctttaggt ggcagcgtga
    50101 caactaatcc attggagatg gctcaggcat atgctgcctt tgccaataat ggagtaatcc
    50161 atcctgcgca cttgattaac cggattgaaa atgccagggg tgaagtgctt aaaaccttta
    50221 ctgataaggc taaacgtgtt gtcagccagt ctgttgcaga taagatgaca gccatgatgc
    50281 taggtacctt ttcaaatgga acagcagtca atgctaacgt atatggctat acactagctg
    50341 gtaaaacagg gacgacagaa accaacttca atcccgactt agcaggcgat cagtgggtta
    50401 ttggttatac gccagatgtt gttattagtc aatgggtagg atttaatcag accgatgaaa
    50461 atcattatct aacggattca agtgcaggca cggcttcagc tatttttagc actcaggcat
    50521 cttacatttt gccttatacc aagggcagcc aatttcatgt agataatgct tacgctcaaa
    50581 atggtatttc agctgtttat ggagtcaatg aaacaggtaa tcaatcagga gttgacactc
    50641 aatctattat tgatggttta agaaaatcag cacaagaagc ttcgcaatca ctatcaaaag
    50701 cagtcgatca gtcagggtta cgtgataagg cccaatctat ttggaaagag attgttgact
    50761 attttagata gcttgcagta ttttagagat cgtgttaaaa taatagttac ggaggcgtta
    50821 tggcacagaa aaaagcgagt ctagcgtgtg tcgaatgtgg cagtcgtaac tactctatcg
    50881 gagtgagtag cacgccaaaa ccaacacgtc tagaagtaaa taaattttgc aaatactgca
    50941 aaacatatac cttacacaaa gaaacacgtt aaaaaggaga gcctgatggg attcattagc
    51001 gggactttta aagtcttaaa agacacaaca tggccaaatc gtaaacaacg ttggaaagat
    51061 tttatctcag ttcttgagta cacagctttc tttacggtga ttatctacat ctttgaccag
    51121 ctcttagcaa agagtgtatt agcactgatt aatttattct aggtcaagat tggtagaaat
    51181 gataagcttg cttggaaaag cctaggctgg gagtattccc agcctttttt gtagtatcaa
    51241 taatctgatt agtaagcttt tttaaagccc atactggttt gagctttaaa aaagcataaa
    51301 ggtaaaggca atgtagcaac gatctccaaa ccaagtgcat taaattagct tgtaacagag
    51361 tcaacgcata aacagcctgg cctcacttgc ttagcacatt aaaaatagca atgaatatta
    51421 tggttgagga aaacagcctg atagtctgct aaatcttcgg gatgataagc cttgacctca
    51481 gccatctcat actccctacc tagaagtttg tacttgtttt ctccaaattg atggaaaggt
    51541 aacagttgca cttggtcaat ctcaagctga ttaaagagct ctgagaaggc tttagcatcg
    51601 tctagggaat cattaaattg gggaataaca gggatgcgca agacgatttc ttttccagct
    51661 tgaaaggcat aatgaatatt tttgataatc aagtcattac gaaccccagt cactttttgg
    51721 tgtctgagct gattataatg tttcaaatcg gtatagataa agtccacgta gtcaataagg
    51781 gtgacaaatt gctcgtgttt ggcaaaggcg gtggtttcaa ttgcggtgtg aagacctgct
    51841 gctttggctg cttttaacag ggctaaggca aagtcaaact gagcaaagat ttccccccct
    51901 gacagggtca tgcccccacc tgactcttcg tagaaatcaa ggtctttaag gacttcttcg
    51961 atcacctcgt ccacggtttt ttcctctcca actattttag tattaagccc atcacttgtt
    52021 aacatctgct caggcacttt ttgttgggat tcgggattgg cacaccaagg acatctcaag
    52081 gggcaacctt ttaaaaaaac ggttgtccga ataccagggc cgtcatgaat actaaaatgt
    52141 tggatgttga aaacaatacc gcgatcagtc ataagtgtga attcctttcg ttgtaaaacc
    52201 atcataccag ataaacgtaa ataaatcaat tacgaatgaa agtaaatatc ccttttcttt
    52261 taagaaaata actggtacaa tgaaagggag gtgaatgatg aatcgattag aacgtattat
    52321 tcaactggtt tcccagaaga aaaaaataga tgttaacagt ttatcagagc aattagacgt
    52381 ttcgaaagta accattcgaa aagaccttga taagcttgaa agtaagggct tgttgcgacg
    52441 ggaacatggc tatgcggtgc taaacagtgg cgatgacctc aatgtacgct tgtcctacaa
    52501 ttacaacata aagcgacgga ttgcagaaaa agcagctgaa ttggtacaag acaacgatac
    52561 cattatgatt gaatctggct caacctgtgc ccttttggca gaagtgcttt gccagaccaa
    52621 acgaaatatt aaagtcatta ccaattcgtg cttcattgcc aactacattc gtcaatatag
    52681 tagctgtcaa atcattttac tgggcggtta ttaccagccc aattcagagg tgactgttgg
    52741 tcctttattg aaagaaatga tatccctttt tcatgtcaat cgtgtctttg ttggaacaga
    52801 cggtttcaac aaagatcttg gctttatggg caaggacatg atgcgctcag aaggggtgcg
    52861 ttacatggca gatgctgctg aggaagtggt gattctgacc gattccagca agttttcaaa
    52921 aaccagtctg gtacaccagt tgtcattagc tgatgtcaat cgcgtcatta cagaccaagc
    52981 actagacaag caaacacaag agttattaag tgctagtggt cttgttcttg attttgtttc
    53041 ttagaaaata ggtggctaga tgaaagaaga acgcagacgg ctccttgcca aggtggctta
    53101 cttgcattat gttcaaggca agagccaaac cttgatttca aaggaaatga atatctaccg
    53161 aacaacggtt tgtcggatgt tagcaaaagc caaggaagaa ggcattgttc ggattgaaat
    53221 tgccgactat gatgctgatt tatttgcttt agaagaatac gtgcgacagc agtatggctt
    53281 ggaaaaacta gaccttgtgc ccaaccaggt tgaagatacc ccaatggata ccttgacaaa
    53341 tgttgctaaa acagcagctg aggtttttag acatgttgtt aaagatggcg ataagattgg
    53401 tctgtcctgg ggagcgactc tttcttgctt gatggatgag ttaaatccaa aagcgatgaa
    53461 agatgtgttt atttatcctt tggcaggagg acctagccat attaatgcca aataccatgt
    53521 caataccctg gtttaccgct tggctcgcat ctttcatggt aatagcgctt ttatgaacgc
    53581 tatggtcatt caagaagaca aacacttggc taagggaatc ttacaatcta aatactttaa
    53641 cgacatctta acaagctggg accagctaga tcttgcctta gtaggaattg gtggggagcc
    53701 taatagcctt gagcagagtc aatggcgtga tttattaacc agctcagatc atgaccaact
    53761 caaatatgaa aaagccgttg gtgaagtttg ttgccgtttc tttgatcagg caggtcagcc
    53821 agtttacact ggcctacagg atcgaactat tggtatttcg ctagaacagt tgaggcgtgt
    53881 ccccaaaaca atggctgttg caacaggtaa acacaaggct aaggcaatat tggcagcctt
    53941 aaaagcaggt tttatcaatt atctagtgac ggataaagag accatgctag ctgttttagc
    54001 gttagatgaa gatattgatc ttaataacgt tcttctttaa tagaaattca aagatactat
    54061 gatatgtata gaggcccaag accaaacgtc agggcttttt ttgtttgatg tgttgatgaa
    54121 acagagattg ctcaaatgtg cagttaatgt attggcacaa aagtgcatta accaaaatat
    54181 agcacaaaat atattgaccc taaaaacaaa aatgatacaa taaacttacc aacgaaatgt
    54241 aaacgattac aaacacaata acatcacctt cgaaaaaaag gctcattgcc gttacatcac
    54301 ataaaaaaga aaaaaaggag acaccaatgc aagttattgt accagaccaa atcattatgg
    54361 gcttaatttt aaatgcaggc gatgccaaac aacatatcta ccaagctctg aaatgtgcta
    54421 aagaggacga ttatgccact agtgaaaaag agatggcctt agcagatgat gccttattag
    54481 aagcccacaa tttacagacc cagtttcttg cccaagaagc tagtggtaat aagtcagaga
    54541 ttacagctct ttttgttcac tcacaggatc acttgatgac gaccattaca gaaattaatc
    54601 tcgtcaagga gattattgac cttcgtaaag aattggcaac caaataatat ttcattcata
    54661 aaaaggagat tgacatgatt aaaattggat tgttttgtgc agcaggattt tcaaccggta
    54721 tgttggtgaa caacatgaaa gtagctgctg aaaaaaaagg cattgattgt cagattgaag
    54781 cctatgctca agggaaatta gcggactatg caccattact tgatgtggca cttttaggcc
    54841 cacaggttgc ctatactctg gatcaatcag aagctatttg taaagacaat gatatcccca
    54901 ttgcagttat cccaatggct gattatggaa tgttagacgg caacaaggtg cttgatttag
    54961 cccttagtct tgttaaagaa taggagtcaa tcatggcaaa aatgaatatg caaaacatca
    55021 tcatgccaat catgaaattt gtgaatatgc gcggtatcat cgccttaaaa gacgggatgt
    55081 tagctatttt acctttgacc gttgtgggaa gtcttttcct gattgctggt cagattcctt
    55141 ttcaagggat taatgatgcc attgccggtg tgtttggtgc cgactggaca gaacccttta
    55201 tgcaggttta ccatggaacg tttgctatta tgggcttgat ttcctgcttt gcgattggtt
    55261 attcttatgc taaaaattca ggtgttgaac ctctcccttc aggggttcta tccttatcag
    55321 ctttctttat tctattgaga tcatcttatg ttccagcaga aggcgaacct gtcggtgatg
    55381 ctattagcaa ggcttggttt ggtgggcaag ggattattgg tgctattgtg attggtttaa
    55441 cggtaggtgc tatttataca gcatttatcc gtcgccacat tgtcatcaaa atgccagagc
    55501 aagtgccaca agccattgcc aaacagtttg aagctatgat tccagccttt gtgattttta
    55561 ccttgtcaat gcttgtgtac attattgcca agtcagtgac aggtggtggc acctttattg
    55621 aaatgattta tgatgtcatt caggtaccgc tgcaagggtt aacgggttct ctttatggcg
    55681 cactaggtat tgccttcttt atttcctttc tttggtggtt tggcgtgcat gggcaatctg
    55741 tggtcaatgg aattgtcact gctcttctct tatcaaatct agatgccaat aaggccttga
    55801 tggcagcagg tgagttatcc ctagacaagg gtgcccatat tgtaacccaa caatttttag
    55861 actctttctt gattctatca ggttctggca ttacctttgg tttagtggta gctatgatct
    55921 ttgcggctaa atccaaacaa tacaaggctt taggtaaggt tgcagccttc ccagccctct
    55981 ttaatgttaa tgaaccagtt gtctttggtt ttccaattgt gatgaatcca gtcatgttct
    56041 tgccatttat cttggttcct gtcttggcag ctctcacagt ttatggcgcc attgctattg
    56101 gtttcatgca gccctttgca ggagtgaccc ttccgtggtc aacaccagcc attatctcag
    56161 ggttcatggt tggtggctgg caaggagcta ttgtgcaaat tcttatactc atcatgtcaa
    56221 cgttggtgta cttcccattc tttaaaatcc aagataatat ggcttatcaa aatgaacaag
    56281 ctagtgaaga gtcatgattt agcgtccaca caaattcttg agaccatctc cagttagagg
    56341 tggtttttta gaaaaagaag agcgacttat caggcctata ttgcacaata gtgcaaaata
    56401 aagtgactat ctcttcttat ggttaaggat acgcaaaagt cgcaatatat gataaaatag
    56461 ttacgaaaga aacatttata atttcgtaag aaagaaggag accccgcatg actgaaacaa
    56521 aaagccctta ttttggacat ttaacagaca gaatgactca ctatcgtgaa gctgtgttag
    56581 acaagaaacc ttatattgat gctgagcgtg ctattttagc cactgaagcc tatcaaaaac
    56641 accaaaataa gccagcaaac ctcaaacgcg cttacatgct tcaaactatt ttagaaaaca
    56701 tgaccatcta tattgaggat gaaagcctca ttgctggtaa ccaagcctct tcgaacaagg
    56761 atgcgccgat tttcccagaa tacactctgg aatttgtcct caacgaactt gatctttttg
    56821 aaaaacgaga tggggatgtc ttctacatta ccgaagaaac caagcaacag ctccgagaca
    56881 ttgccccatt ctgggaaaac aataacctgc gtgctcgctg cggtgtcttg ctaccagaag
    56941 aagtgcaagt gtacatggaa acaggtttct ttggcatgga aggcaagatg aactctgggg
    57001 atgcccattt agcggttaat taccaaaaac tcttggaaca cggcttaaag ggatttgaag
    57061 aacgggcacg agctgccaag gctgcgcttg atctgaccat tcctgaaaac attgacaaat
    57121 accattttta cgactctgtt tttattgtca ttgatgcagt caaaacttat gctaaacgct
    57181 atgccaagtt ggctagagaa ctagcaaaaa cggctaaacc agaacgtcaa gcagaattgt
    57241 tagacatcgc acgcatttgt gacaaagtgc cttacgaacc agcgaaaacc tttgcagagg
    57301 ctgtacaatc cgtctggttc attcagtgta tcttgcaaat tgaatcaaac gggcattccc
    57361 tatcttatgg ccgctttgac cagtacatgt atccttatgt caaagctgat ttggaggctg
    57421 gccgtgaaac agaagcctca atcgttgaac gtttgaccaa cctctggatt aagaccttga
    57481 ccattaataa agtacggagc caagcccaca ccttctcatc agcaggtagt ccactttacc
    57541 aaaacgtgac gatcggcggt caaacacgcg ataaaaaaga cgcggtcaac ccactgtctt
    57601 accttgtcct acggagtgtg gctcaaacca aattgccaca acctaacttg actgttcgtt
    57661 accataaagg cctagacaat acctttatga acgaatgtat tgaggtcatg aaacttggtt
    57721 ttggcatgcc agccatgaac aacgatgaaa tcatcattcc atcctttatc aaaaaaggtg
    57781 tctcagaaga agacgcttat gattattcag ccattggttg tgtggaaaca gcagttcctg
    57841 ggaaatgggg ctaccgttgc acggggatga gttacatcaa cttccctaaa atcttgctga
    57901 ttaccatgaa tgacgggatt gacccagcat caggcaagcg ctttgcaaaa ggacacggtc
    57961 atttcaaaga catgacctct tatgaagaat taaaagcagc atgggatgcc accctgcgcg
    58021 aaattacccg catgagcgtc attgttgaaa atgccattga cctaggtctt gaacgtgaag
    58081 taccagatat tctttgctca gccttgacag atgattgtat cggtcgtggc aagaccttaa
    58141 aagaaggcgg agctgtttac gactatatct caggccttca agttggtatt gccaacctat
    58201 cagactccct agcagcgctg aaaaaattag tgtttgaaga aggtcaatta actccagaag
    58261 aactctggca ggcccttgaa agtgactttg ctggagagcg cggagaagag atccgacaaa
    58321 tgctcatcaa tgacgcacca aaatatggta atgatgatga ctacgcagat agtttagttg
    58381 tcgaagctta cgacacctac attgatgaaa ttgccaaata cccaaatacc cggtatggcc
    58441 gtggcccaat cggaggtatc cgctattctg ggacatcatc aatctcagcc aatgtcggac
    58501 aaggaaaagg gaccttggca acaccagacg gtcgccatgc agggacacca ctagcagaag
    58561 gctgttcacc agaacacagc atggacaaaa aaggcccgac ttcagtattg aaatctgttg
    58621 ctaaattacc aacagatgag attgtcggag gagtgctact caaccaaaaa gttaacccac
    58681 aaaccctggc caaagaagaa gacaaactga aattaatggc cttacttcgt actttcttta
    58741 atcgcctaca cggttatcat atccagtaca atgtcgtgtc acgtgaaacc ttgattgatg
    58801 cccaaaaaca tcctgaaaag caccgtgacc tcattgtccg tgttgccggc tactcagctt
    58861 tcttcaatgt gctctcaaaa gcaacccaag atgacattat tgaacgtaca gaacacacac
    58921 tttaggagaa aataagtatg gaatacatgt tagacacttt agatttagaa gcgattaaaa
    58981 aatggcatca cattttgcct ctcgcaggtg tgacctcaaa cccttctatt gccaaaaaag
    59041 aaggtgacat tgatttcttt gaacggattc gagaagtccg tgctattatc ggtgacaagg
    59101 cttctattca tgttcaagtg atcgcccaag attacaaagg catcttaaaa gatgctgctg
    59161 aaatccgcag gcaatgtgga gatagtgtgt atgtcaaagt acctgtaacc acagagggcc
    59221 ttgcagccat taaaaccttg aaagctgaag gctatcatat taccgcgaca gctatttaca
    59281 gcactttcca agggttatta gccattgaag ctggcgctga ttatttggct ccttattata
    59341 accgtatgga aaatcttaat attgatccag aggccgtgat tgggcaattg gcagaagcta
    59401 ttgaccgtga gtgttctgac agtaaactct tagcagcgag ctttaaaaat gtggctcagg
    59461 tcaataaatc ctttgctcta ggagcacaag ccatcactgc tggcccagat gtgtttgaag
    59521 caggatttgc catgccatct attcaaaaag cggttgacga ttttggcaag gattgggaag
    59581 ccattcatca ccgcaagagc atttaagaaa tagcatcagt agtaatcaca tcattaggaa
    59641 tcacagataa aggagttcaa cgatgaaagt atttgcaagt ccatctcgtt acattcaagg
    59701 taaaaatgcc ttgtttacca atgctaaaac cctaaaacaa ctaggaaata gccccatttt
    59761 actgtgtgat gacgtggtgt atggcatcgt cggagaaaga tttgaaagct atttgataga
    59821 taatggcatg actcctgttc acgtagcctt taatggcgaa gcctcagata atgaaatcag
    59881 ccgcgtggtt gccattgcca aggaaaatgg caatgatgtt attattggac ttggtggtgg
    59941 aaaaaccatt gacagtgcta aggctatcgc tgacttactt gctgttccgg tgattattgc
    60001 cccaaccatt gcttcaacag atgccccaac ctcagcctta tcagttattt acaccgatga
    60061 aggagccttt gaaaaataca ttttctattc aaaaaatcca gatcttgttt tggttgacac
    60121 acaagtgatc tgtcaggcac caaaacgctt attggcgtct gggattgcag atgggttagc
    60181 aacatgggta gaggcgcgtg ctgttatgca aaaaaatgga gacaccatgg caggtggcaa
    60241 tcaaaccttg gcaggagttg ccattgccaa agcctgtgag cagaccttgt ttgcagatgg
    60301 cctaaaagcg atggctagct gtgacagaca agtcgtgacc ccggccttgg aaaatgtcat
    60361 tgaagccaat acacttctta gtgggcttgg ttttgaaagt gcgggtctgg ctgctgcaca
    60421 tgccattcac aatggcttta cagcgctgac aggtgctatt caccacctta cccatggtga
    60481 aaaagtcgcc tatggtacgt tgacccaact cttcttggaa aatcgttcgc gtgaagaaat
    60541 tgaccgttac atcgactttt accaagccat tggcatgcca acaaccctca aagaaatgca
    60601 tttagacacg gctactcaag aggatttctt aaaaattggc cgtcaagcaa ccatggcagg
    60661 cgaaaccatc catcaaatgc cgtttgttat cagtccagaa gatgtagcag cggctttggt
    60721 tgctgttgat gcctatgtta cgagtcgata ggctaaccaa aaagagattt cttatttgcc
    60781 agataacctc aaaaaaacaa gtctagggag tcacttccta gacttgtttt gcttattgtt
    60841 ggttggtaat agcactttta gcggggtcat tgtgggcaat acgactggca gctgcagcag
    60901 caatggcgct aacaatatca tctaaaaagg tatggcagga gttgccatct ttatggttga
    60961 gtttgtcaac aattcctggt tttgtcttgt ccagataccc ataattggta aaaccgatag
    61021 acccatagag gttaacaatg gataaggcca ggatttcatc aatcccatac agcccttggt
    61081 cggttttcaa gatactaagg agtggctcag acagctggtt ggcttcagcc aatttatcta
    61141 attctacccc agtgataata gcgttttgca cttctcgttt tgcaagcacc gcctcaacgc
    61201 tctctaaaca ttctgccatg gtcaaattgg gaatataatc attctgcaaa aaaagcacca
    61261 gctctgcaat agcttctaaa gaaccccctc gctcagccag taattgataa gacacttctc
    61321 gtaggtgatg gtcagttgac atgtgagacc tccttttttt ctttattgta acataaaaga
    61381 tttcgagaaa cctctacaac agctaaagag gggatagttc ttttttaaga gatgttgact
    61441 actgagatcc cctacttggt aaaagtcaaa gaaacgatag taaagtaacc atcttaccta
    61501 ggcctaaata caatcaccac ccctaggtga cacacagcac ctatttcaaa aatgatatta
    61561 gtatggcaaa acaaaaagac aacgccttct tttttctcct tactatctcc tttaattttc
    61621 atatttttta aaaaaactat tgataaacta gttaagtaag cgtatactat ggttagtgag
    61681 cgaaattaga aaagaggaca agcatatgaa tctacttgga tcaagacggg ttttttctaa
    61741 aaaatgtcgg ttagtgaaat tttcaatggt agctcttgta tcagccacaa tggccgtaac
    61801 aacagtcaca cttgaaaata ctgcactggc acgacaaaca caggtctcaa atgatgttgt
    61861 tctaaatgat ggcgcaagca agtacctaaa cgaagcatta gcttggacat tcaatgacag
    61921 tcccaactat tacaaaacct taggtactag tcagattact ccagcactct ttcctaaagc
    61981 aggagatatt ctctatagca aattagatga gttaggaagg acgcgtactg ctagaggtac
    62041 attgacttat gccaatgttg aaggtagcta cggtgttaga caatctttcg gtaaaaatca
    62101 aaaccccgca ggatggactg gaaaccctaa tcatgtcaaa tataaaattg aatggttaaa
    62161 tggtctgtct tatgtcggag atttctggaa tagaagtcat ctcattgcag atagtctcgg
    62221 tggagatgca cttagagtca atgccgttac agggacacgt acccaaaatg taggaggtcg
    62281 tgaccaaaaa ggcggcatgc gctataccga acaaagagct caagaatggt tagaagcaaa
    62341 tcgtgatggc tatctttatt atgaagccgc tccaatctac aacgcagacg agttgattcc
    62401 aagagctgtc gtggtatcaa tgcaatcttc tgataatacc atcaacgaga aagtattagt
    62461 ttacaacaca gctaatggct acaccattaa ttaccataac ggtacaccta ctcaaaaata
    62521 ataccaaaag gctagacctc tgctcactag gcctagcttt ttacatcaaa aaaagcaatg
    62581 actatagaaa gtaaaaatac tagaaaaagc aatgattgcc gtcattgctt tttatgattt
    62641 gtcaaaaagt aaaaagcaaa ttgaaaaaat tgccctggag ctattgagat aaactacacc
    62701 atttattaag ataccaaatc aaaaagctaa caccataaga gcgttagctt tttaaagagc
    62761 ctcaggacag tttatgttta atggcttcta ggtaggtttg aaacatatga tggatcgttt
    62821 tgcaattaag taattggaag atctccaaga tctgcacaca cttttgctca gccccctctt
    62881 taccatttat gaggccaata atgccatcaa aaaaagacac acaacaccgt tcatataagc
    62941 aatcatcatt tagctgataa tcttcaattt ttgccaaaat ctcagaagct ttatcatact
    63001 cttgtcgctg aataaacaaa atcaacatat tgacaaacat ccgaatcgat tcattcccat
    63061 aataccttag ggtattgtat ttatcgaggt tcaaaataac ttttgaaaac accatctcaa
    63121 taaagcaaga ctcaaaaata aacatacaat tattaaaaag cacagtctca tagtgactcc
    63181 aagtttcaat attaataaga tagtttgaca aataagtccg ctctgtcaga cagtcttcct
    63241 cagtaagagt tgctaataac accttgacca aggcaaaaag gttcttttcc tttgttgatt
    63301 tactatcttt ataagaatca aggatatgat ttaaaccatc tatgtttcgg cattcaaaat
    63361 aatgttttgc cgtatccata tcaataaatt ctttatattg tttaaagtta ttactaataa
    63421 agagaaactc atcaacattg acattcaagt tgtctaagat ataaagtaac tttttcgaag
    63481 aaatgtcaag attattttta ataaaacgat aataggtttg cctagtgaga taatcaccac
    63541 aaacttgttt aatcgaaatg ttttttgaat gcctaatgaa ttcaacggtt tcaccaattt
    63601 ccatatgtca agccttccta gttgatgtca aaaatacgtt acgcatgtac ctaatacgta
    63661 acaagttgaa tgtttcggat gatagtcgct tatgataggt gcataaggtc aatagccaga
    63721 tgcgatagtt ttatcaactg tcatatgtta aacctttcta atctaataga tgttaaaaat
    63781 acgttacgtg tgtacctaat acgtaacaaa ataatgggtt agcaaaataa gcagctatga
    63841 tatagccata aggttaaaag gaggcgccta ctatgtggtt attgttacta tttttgtagt
    63901 tccttttgca aaatggtagt agataaaaat gagaagtcaa taaaagcatt gacaaacaaa
    63961 agaatcattt tgtttatatt atcagtattt ttaagtttct tgtcaactga aatgagcatc
    64021 tactagccac aatagtaact aaaaggctaa ttgtcagtaa aaatgagata aatcagccag
    64081 gaaaaagcct acgagtcaac taatggacaa cccaaaaaaa gacaaaggat tttcacttgg
    64141 tgttgtggac cctatcacga tccttatcac agaatcagtt attaacaaaa tgagaacggt
    64201 cccatatcat ctaaaaaaac tgtcaagcac aacctattgt ttgctagctg atcttgccta
    64261 tcagtctgcc tagcagatac ggcagaagat aagagatcaa ccaaaagatg ataagtgcaa
    64321 cgactcatag cgtcttttga ccaaaaccaa ttaggctcac atgagatgag aataattggg
    64381 ttgggttgtc agtgtcaact aaccgtgtta ttgtctatta ccattcatgg tatcagcgac
    64441 atcgtatgat aaccatacga ttcagctaag taaggaggtg tgtccaatgt accgttaaaa
    64501 gcaaatgcag tagattaact tattttgaaa gaggtataaa aaaaatgaat aaaaagaaat
    64561 taggtatcag attattaagt cttttagcat taggtggatt tgttcttgct aacccagtat
    64621 ttgccgatca aaactttgct cgtaacgaaa aagaagcaaa agatagcgct atcacattta
    64681 tccaaaaatc agcagctatc aaagcaggtg cacgaagcgc agaagatatt aagcttgaca
    64741 aagttaactt aggtggagaa ctttctggct ctaatatgta tgtttacaat atttctactg
    64801 gaggatttgt tatcgtttca ggagataaac gttctccaga aattctagga tactctacca
    64861 gcggatcatt tgacgctaac ggtaaagaaa acattgcttc cttcatggaa agttatgtcg
    64921 aacaaatcaa agaaaacaaa aaattagaca ctacttatgc tggtaccgct gagattaaac
    64981 aaccagttgt taaatctctc cttgattcaa aaggcattca ttacaaccaa ggtaaccctt
    65041 acaacctatt gacacctgtt attgaaaaag taaaaccagg tgaacaatct tttgtaggtc
    65101 aacatgcagc tacaggatgt gttgctactg caactgctca aattatgaaa tatcataatt
    65161 accctaacaa agggttgaaa gactacactt acacactaag ctcaaataac ccatatttca
    65221 accatcctaa gaacttgttt gcagctatct ctactagaca atacaactgg aacaacatcc
    65281 tacctactta tagcggaaga gaatctaacg ttcaaaaaat ggcgatttca gaattgatgg
    65341 ctgatgttgg tatttcagta gacatggatt atggtccatc tagtggttct gcaggtagct
    65401 ctcgtgttca aagagccttg aaagaaaact ttggctacaa ccaatctgtt caccaaatta
    65461 accgtagcga ctttagcaaa caagattggg aagcacaaat tgacaaagaa ttatctcaaa
    65521 accaaccagt atactaccaa ggtgtcggta aagtaggcgg acatgccttt gttatcgatg
    65581 gtgctgacgg acgtaacttc taccatgtta actggggttg gggtggagtc tctgacggct
    65641 tcttccgtct tgacgcacta aacccttcag ctcttggtac tggtggcggc gcaggcggct
    65701 tcaacggtta ccaaagtgct gttgtaggca tcaaacctta gtatggaaat gcatttcgtt
    65761 agaacagaac ctgaggcacg ccgcatagct gaaacctttt gtgccgaaaa cacacaaaca
    65821 aaaaccccta tgcgcgtgca gcagttaagc tatccatcag acacagatca ctcaggtggt
    65881 gagctataca tctatgcttt gtctcctgct ggatttatca tcgtatcagg agacaccaga
    65941 gcgcacacca ttttaggcta ttcttttgat aataacctgg acctcaacca tgataatgtc
    66001 agaagtatgg tagaagctta ccaaaaacaa atcaactctt tggattgatc ggtcaaagta
    66061 gcttttgccc cctcaccact cgtttaagac gaccgtgttt ctaaaatacc actacctttt
    66121 aagtttcacg ccggcctaag caagtgtttg tctcatggag cagactgttt ccatcgaaaa
    66181 gtcacccatt gataaaggag gtcatcaacg cctactaatg atgacttccc taagatgcca
    66241 aggtaaagga ctgagttctg tagagatggg actcagtcct tttacgattt ttgggagtta
    66301 ttctagccat accgtggcaa gaaaacaaca ctcaaaaata gccaacgtca gtttctgttc
    66361 atacatgact acagccaaac accacaacct aacctatcct aaacacagtg gctttctaac
    66421 gaacagtcac aacgagcagc aacaccaaaa agcaagatta agctacgttt aagactaatg
    66481 atataagata acagtaatta ttaatgagaa aagaggctca aaaggacatg aaacaaatga
    66541 ataaactcat tacaggagtg gtaacgctgg cgacagtcgt gaccttatca gcttgtcaat
    66601 catcacacaa caacaccaaa ctcgtctcga tgaaaggaga caccatcact gtcagtgact
    66661 tctacaatga gaccaaaaac acagaactcg cacaaaaagc catgttaagc ttggtgatta
    66721 gccgtgtatt tgagacacaa tatgccaaca aagtctctga caaagaggtt gaaaaagcct
    66781 ataaacaaac cgcagaccaa tacggtacct cctttaagac agtcctagca caatcaggct
    66841 taacgccaga aacctataaa aaacaaattc gcctcacaaa attagtcgaa tatgccgtca
    66901 aagaacaagc caaaaacgaa accatctcaa aaaaagacta ccgtcaggcc tatgacgctt
    66961 ataccccaac catgaccaca gaaatcatgc agtttgaaaa agaagaggat gccaaagcag
    67021 cccttgaagc cgtcaaagct gaaggggcag actttgcagc tattgccaaa gaaaaaacca
    67081 ttgcagccga taaaaaaata acctacacgt ttgactcagg cgaaacaacc ctaccagcag
    67141 aagtagttag agctgcatca ggcctcaaag aagggaacag atcagaaatc atcacagcgc
    67201 ttgatccagc cacctcaaaa cgcacctacc atatcatcaa agtcaccaaa aaagcaacta
    67261 aaaaagcaga ctggaaagcg taccaaaaac gcttgaaaga catcattgtg actggcaaat
    67321 taaaagaccc tgacttccaa aacaaagtca tcgctaaagc tcttgataaa gcaaatgtca
    67381 aaatcaaaga caaagcattt gccaatatct tagcccagtt tgcaaaacct aaccaaaaac
    67441 aacctgccca aaaatagaag gttgccagtt tatttcccta aggttgctta aaaagacagc
    67501 cctagccatc actccccttt tcaaaacacc gataatggaa acgtgtccta tactgatggt
    67561 ctagggcaat gtcataaagc taaagactct atccaaataa ggatagagtc tttaatgttt
    67621 taaaaccagt cagcaaaaaa attcacgcta agcctagaat gaggcttagc gtgaatggta
    67681 tcagtgatag tccgttaaac tagctgattg ggagtcagaa actctcctta cccaatctga
    67741 ctataccccc agtaccagag gcgtctcatc gcagaagtag taatcagata atcgtctgat
    67801 aagctcttgt cctcaagcaa ctgatgagta tcgtcgcacg ggccatgagc tgtcggtggc
    67861 tgatagctag taggctcatc agccaaaact tttttggagt aacgctttgg tctcagacgg
    67921 cgccagaggg ttgagagagg ggtgagaaaa aaacgatggg tcatcttggc ctccttgtct
    67981 aatgatagtc accttaacag acaaaagtgt caataatgtt attttaagga gaaaaagatt
    68041 aaaaatcaag gtcacaaact ttcctttgtt aaaaaaacgc gaccctaata aagactctaa
    68101 atacccctcg catttgagct tctccttgcg cagctatctg cgcactcagt tgcctccaag
    68161 ctaatgatag cgataccatt attgtcctat aggctatcgc ggcaacccct tttttcgtct
    68221 caacgagtct gacacagctg tcatgacaga gaaaagactc ttgtctatct aagagatcat
    68281 taatttttct ctcaccaaaa aactgatttt gggaacaaaa aagtatggtg tataataagt
    68341 ttcggaacaa ttatgacata ataatgaaag taaggttaac gaaacattga caaattgtaa
    68401 gtataaactt agaaagttat ctgtagggct cgtctccgtt ggaacgatgc tgatagcccc
    68461 gacagtttta ggacaggagg ttagtaatac tgaggctagt gctagtagta ccgctagcgt
    68521 agacgctacg gcaagtggga cgactgccag cggagctagt agtgaagcaa ccgtagctac
    68581 taccaatgga ggaagtcagt ctactcaagt agcagctgaa acgactccac aacctcaagt
    68641 acagacttca gaacaaccag cagctacatc cacatcattg gcttcttcta ctagtagtag
    68701 tgaagataaa gctcctaagg cagcaagcac taaatcatct tcagcaactg tggctagctc
    68761 tagtaatggt agcaatcaag gtgctggtac tgaagttgaa ccacagatga tggacgtgga
    68821 acagtataag gttaataagg aaaaaacaga gcttactgtt aaagatgaca accagcagct
    68881 caagatccgt aaagatgttg atgaattaaa aaataaagat ctttttgatg tcaaacgtga
    68941 agtgaaagat aatggcgacg gaaccttaga tgtaacctta aaagtaacac ctaaacaaat
    69001 tgacgaaggt gccgatgtta tggccctttt agacgtctct caaaagatga cagatgctga
    69061 ttttaacaac gctaaggaaa aaatcaagaa attagtcaaa accttaacga gtaaacctac
    69121 tgatagtcat ccaaaccata atgctcgcaa ctctgtgcgc ctaatgacct tttaccgtga
    69181 ggttagtgac ccaattgata tatcaggaaa gacggatgag gaactcaata agatattaga
    69241 cgatgtgtgg caaaatgcta aacataattg ggactggggg gttgacttgc aaggagctat
    69301 ccataaggct cgagggattt ttaagcaaga ccaagaaaaa aaatcgggta aacgccagca
    69361 tatcgtcttg ttctctcaag gtgagtcgac ttttagttat gatattcaaa agagtgagaa
    69421 agagagagac aacaatttat ctcgtataaa tgaaaaaatt acctcttcta accctttgtt
    69481 acattggcca cctattttta accacacgca tcaaaatgca gatatgttaa atgaaatcga
    69541 aaggattata aagatagggg agcaactagg tataaaaggc ctttctagta taaaagggta
    69601 tttaagtacg gcagcagcag gtagcgggat tctgggaaat tttatcggtg gaggaagtct
    69661 tacagaatac ttaactctta aagaatataa ctctgataaa ttagaagaaa gccaatttga
    69721 ttacaccaaa cgtgtgggcg agggatacta ttattatagc ttctcaaaaa gagtaagtca
    69781 caacgatttc ccttttaaaa ctttaataga gcaggctatc acacaagcga ttcctactaa
    69841 gggtgaaagt aaaaattggt tcgagaaatt tttagataaa ttttcattgg tggaaagtta
    69901 tcgagacact caaaaaaaag cattattaaa gattcttgaa tatctttttt ataagcgtga
    69961 ataccactac tataatcaca atctttcagc gatagctgaa gccaaaatgg ctcaacaaga
    70021 aggcatcacc ttctactccg ttgatgttac tgattcagaa acaactccta aaagagtgag
    70081 gcgacaaaca gcagtacgtc aggaaaaagc gaaaaaagaa tccaaagaag agcgcaatga
    70141 aaagtttgat aaatatctga aaaagatgtc tgaaggtaat actttcttag atagtgttga
    70201 agagaaagat aagttcaaag acaccttcac caatgtaaca gtgacagaaa ccttcgaaga
    70261 taaggtttcc gctatagcaa attcttggga atcttcgcta gatagtcata ataacaaatc
    70321 agacagaaat cataaaggaa gtgtcaaaca tacacaagct caaagcagcg gatggtttgc
    70381 caatacccaa gaaagcctca cttggactat ttccaaagac cagctcaaaa aagcctttga
    70441 agatggagaa ccgctaaccc tcacctataa gcttaaagtt gataaagata aatttagaga
    70501 aactcttaaa aaacaacaag aacgtaatag aaaaaacgag cagcatctcc ggaaagtgag
    70561 aacactgtca cagaaaaaat tatttcaaat aagatttctt acaagattaa taatggtaag
    70621 gatataaata acaataagtt ggaagatgtt aaaatgtctt acagcaagtt caaaatgcct
    70681 ataccagaac ttgatataga agttgtacca ataccagaaa aaccactggt agaaccaatg
    70741 acgcctctat atcctgcaat tcctaattac cctacccctc aacttccaaa agatgaagat
    70801 ctggagatta gtggaggtca tggaccgatt gtcgatatca tcgaagatac tggtacaggt
    70861 gttgagggcg gctctcaaaa cggcgtggtt tcaactcagg aaaataaaga tccaatcgtt
    70921 gacatcaccg aagataccca accaggcatg tcaggctcaa atgacgcgac agttgtcgag
    70981 gaagacacag cacctaaacg tccagatgtc cttgttggtg gtcaaagtga tccaatcgat
    71041 atcaccgaag atacccaacc aagtgtgtca ggctcaaatg acgcgacagt tgtcgaggaa
    71101 gacacagcac ctaaacgtcc agatatcctt gttggcggtc aaagtgatcc aatcgatatc
    71161 accgaagata cccaaccagg catgtcaggc tcaaatgacg ctactgttat cgaagaagat
    71221 acgaaaccaa aacgcttctt ccactttgat aacgagccac aagcaccaga aaaacccaaa
    71281 gagcaaccaa gtaacagctt accacaagct ccagtctata aggcagctca tcacttgcct
    71341 gcatctggag acaaacgtga agcatccttt acaattgttg ctctaacaat tattggagct
    71401 gcaggtttgc tcagcaaaaa acgtcgcgac accgaagaaa actaaccctc gttagtctag
    71461 cgaccctaag cctttggctt taaaaatttc tcattatccc cctcaaacac cggtacctca
    71521 ctgagatggc gcttaaaaaa gccgctagtc ttaaatcagg tatcccctac ggttgagggg
    71581 gattaatggg tatcacaaca agtaaaaagg tataatgatg aaatatgact aaaaaattta
    71641 ttaaatcagc agtgattcta ggcttagcaa gcataaccat cagcatgacg caacccgttg
    71701 aagccatttc aaatagttta gaatatcaag cacaatataa acgtgtcaaa gaagaactag
    71761 acgcattaaa agaaaagggc ccaaaattag gggaacgttc cctttatttt aaagtggtga
    71821 atagatttct aggttggttt ggcgtagtct gggacccact cgcaatcaaa gaaaccgatc
    71881 acctgttgga cctcatcgga caagtcaatt acttggtgag taacaccgag tttcaagccc
    71941 ctgagtatca aaaggctaaa aacaaactag acttagctgt agcaggctat gacctctacc
    72001 tagctagaga gaaaaaacgt ggtgatgctc ctgattacca agtgacccag ctaacgctag
    72061 gacagctcga aaccatcaaa acgaagatac aagaggccct gaaaacattt gcacaagagg
    72121 tcaaaacgat ccagcaaaaa aaaccagacc tatttcccca tgcttatgtt ttaaacgcta
    72181 agagcaaaga tatcacagat aaagcgcaac cctttttagt gaatggttac tacgatgtct
    72241 tttataagca gtttttattc actaatacaa atgacggcta caactataat aaagaagcag
    72301 acaatctggc ttcgtatcaa aagctgatta aagagctatt aggcttaaaa gctgagatcg
    72361 atcagttaac aaaagacgat cctaaaaacg atcgtttaga gacgttaatg tgggaatcaa
    72421 agttcacctt atctgattca cggagaactt cacgccttag cgcctactat acacaagaag
    72481 attatgtcaa aaaggtggtc ctatctgaag aagctcccaa cttgagaaca gcaaaagaaa
    72541 aggtcaagac cttaaaaggg atcttgcatg attactatgt tgacattaaa gagccagaaa
    72601 aagctaaaaa gtatcgtgtc gaggacgaga ccttgccgtc tcaacctgag gcccctgcta
    72661 aacctgaagc tcctgctcct agtcctagtc ctgctcctgg tcaaaaacca gcagaagatg
    72721 agagatcatc acaagctacg gagccagcta acccgtcaaa agagaattcc tcaacggacg
    72781 catctcaggg atctcacgat agtgaaaacc cagcgacaga cagtccttct caacctcagg
    72841 catcagacca aggcaatcac caaagccaag tgcctaacgc caagcctcaa acggacaaga
    72901 cggacactcc taacgcccca gtccctccac agaacactcc taacgcccca gccatcccac
    72961 aggacacccc aaaagttcct gaggctggtg gtcagagtgg ccccgctggg aacgctgagg
    73021 agaaagctcc tgactctgga cctaaagagt ctggtcaaag ctcatctaaa gagtccccat
    73081 ccgttggcga agacacgacc cctaatcagc cagacgtcct tgtaggtggt caacgtgagc
    73141 caattgatat caccgaagac acaatcactg acgctcctcc gacagtatct gggcacaacg
    73201 catcaacaca gccccaatct gtggtcgaag atacagcacc tcagcgccca gatgtccttg
    73261 taggtggtca aagcgagcca atcgatatca cccaagatac ccaaccgggc atgtcaggct
    73321 ctaatgacgc taccgttatc aatgaagata caaaaccaaa acgcgtcttc cactttgata
    73381 acaaagagcc acaagcatca gaaaaagcgg cagagcaaaa acttgctcct catgatagcc
    73441 acacaacccc tcaaacttca gatgatacag cagcccctca cttgcctgca tctggagaca
    73501 agcctgaaaa agtgtttgca cttgctgcct tgacaatggt cgcagctgca gggctactca
    73561 gcaaaaaacg tcgcaacacc gacaaaaact agtcgctgtt agacacaacg catcactaag
    73621 gccagtgcat ttgcactggc cttagttagg tttttatcaa atagagggtt tttcatgaca
    73681 attgctagca tttcggtgta aaataaaggt aataatcagc aaaggagcaa cccatgacaa
    73741 agcaagatca gttaatcgtt gaaaaaatgg aacaaaccta tgaggccttt agcccaaagc
    73801 tagcaaatct catagaagcc ctagatgcct ttaaagagca ttatgaggaa tatgccacac
    73861 ttagaaattt ttacagtagt gacgaatggt ttcggcttgc aaaccaacct tgggatgata
    73921 tcccgtgcgg tgtcctatct gaagacctcc tctttgatat gatcggtgac cataaccaac
    73981 tactagctga tatcttagac ttggccccta tcatgtacaa gcatatgtaa tcatcgctaa
    74041 cgtcacagat actacgatga taggacaaaa aaagcttgaa aatctcaaaa aaaacagata
    74101 aaaaacagat aatcgttaga aaaacagtag atgcgtctgt taaactaaag gtatcaaaac
    74161 aaaaggagca tttcatcatg aaattcaaaa aagtattagt cattcctgca ttagcattag
    74221 cagcaacatg ctttttaaca gcttgtggca caaagaaaga ttctaaaaaa gaagaagtta
    74281 aagaaatcaa aatgtctgac atcaaagacg acgcagttag caaaaaaaca aaagtggttg
    74341 atggcgaaga agttacagaa tacaccacaa aagatggaaa cgtgattcag attcctgcag
    74401 gaaacgaaga aggcatggaa tcaaaagatg ctggtggatc aggcgctcct gctaaaaact
    74461 aagggttaca acaaagagca taaggtgact cgcaaacgtt acgcatgaca tgaggcagtc
    74521 tgcctaagat ggtaactcac ttatcataat gcctaaatag ccaaacacac taactcgcaa
    74581 atgtacaaaa tcatataccc gtgcttatgt gagcgaacgg ttatatgatt tttgttttcc
    74641 aaaaacaaga aaagaacgct aaaatagcca gtttgtcgtg tttcaagcta cgaaaaagga
    74701 agtgctaaaa tgaaaaaaag atccattata cctgtcatct tactgttggt catcggatta
    74761 atcgttggtg ttaagcgtca tcatgactag taggtcttgc tagtgaccgt tcaaaaggga
    74821 taacgttggt atggtataac cataaaaatc gtataaaaag aaaaaatagc tattgaggag
    74881 aaagtatgtt tcagttaaga aaaaaaatga cgcgcaaaca attagccttg ttgagtgctg
    74941 gagtgttgac ctgtgtggtt ggtggtacct acttgataat gaatcatcaa caacaagaaa
    75001 ttgtctctag tgtcaacaaa gtaaaagcct taaccataaa agaggccatg gaacaaggaa
    75061 aagatatcag cttgacctta gctggcgaag taaccgctaa caacagcagc aaagtcaaaa
    75121 tcgactcaag taaaggagaa gtcaaagatg tctttgtcaa aaaaggcgat gttgtcaaag
    75181 taggacaacc cttgtttagc tatgaaacgt cacaacggtt aacggctcaa agttcagaat
    75241 ttgatgttca aaccaaagcc aatcaactcc aagttgctaa aaccaatgca gcattgaagt
    75301 gggaaaccta caatcgcaag gtcaatgaga tcaataccct aaaatctcgc tacaacactg
    75361 caccagatga gagcttacta gagcaaattc gcagcgcaga agacagtgta tcccaagcac
    75421 taagcgatgc caaaacagca gatagcgatg tcaaaaccgc tcaaatcgaa ctcgataaag
    75481 ctaatgctac tgccacaatg gaaaaaggta aactagagta tgacaccgtt aagtcggaca
    75541 ccgcaggaac cattgttagc ctaaatactg atttgccaaa tcaatcaaaa tccaaaaaag
    75601 aaaatgaaac ttttatggaa attatcgaca aatcaaaaat gttagtcaaa ggtaacatca
    75661 gtgaatttga ccgtgacaag ttaaaaatcg gtcaaaaagt cgaagtgatt gaccgcaaag
    75721 acaactctaa aaaatggact ggaaaagtaa cccaagttgg caacctcaaa gcagaggaaa
    75781 aaggccaagg tcaaggccaa ggtggcaatg accaacaaga caatccaaac caagcaaaat
    75841 tcccttatgt tattgaactt gaccaatcag acaagcagcc actcattggc tcacacacct
    75901 atgttaatgt gctcaacaat gttccagaag ctggcaagat cgtattgaaa gaaaccttta
    75961 caatggcaga aaatggaaaa acctatgtgt ggaaagttga taaaaacaag gtcaaaaaac
    76021 aagaagtcaa gactaagccc ttctcaaaag gttatattga ggtgacaagt ggcttgacta
    76081 tgcaagataa gattgctcag ccgcttcctg gcatgaaaga tggtatggag gtaggaagta
    76141 ttgttaaacc ttaaagatat tcgaaaaagc tatcatcttg ggactgaaga atttgcgatt
    76201 ttaaaaggaa tcgatttaga agttaacgag ggtgactttt tagccatcat gggaccatca
    76261 ggttcgggaa agtcaacatt gatgaatatc attgggtgtt tagataagcc tggctctggc
    76321 tcatatgcca ttgaaggcag agacgtgtca tccttatctg ataatgaact tgctgatttg
    76381 cgtaatcaaa aaatcggctt tgtttttcaa aactttaacc tgatgcccaa gctaacagct
    76441 tgtcaaaatg tcgaattgcc cttgacttat atgaatgttc ctaaaaaaga gcgtcgcaaa
    76501 cgagccctag agatgttaaa actagtagga ctagaagaac gtagtgaatt taaaccgatg
    76561 gagctatctg gtgggcaaaa acagcgtgta gcgattgcaa gagctttagt cactaatccg
    76621 agttttatcc ttggtgatga gccaacaggt gcactagaca caaaaaccag cgtccaaatc
    76681 atggacctat ttaaacaatt caatgataac ggcaaaacga ttattatcat cacacacgag
    76741 cctgaagtag ctgccttatg caaaaagacg gtgatcctaa gagatggtaa tatagaacat
    76801 tccgatatag agtaaagggg gaaaataatg gaagatatta ttgcagcttt aggctctata
    76861 ttatcacaca agatgcggtc aattttgaca atgctaggga ttattatcgg tattggtgcc
    76921 attatcgcaa tcttttcaat catcgaaggt aataccgaaa ataccaaaag acagttgatt
    76981 ggtggcagca ataacactat taacattgtg tttaacaaaa aaagcagtat tgatcctaaa
    77041 tttccagata agtcaaatgc taaaaaacca gactatcttc cttttatggc tgaagaagag
    77101 cttagcaaaa tacagcaagt caaaggcgtc aaaaacgccc tgatctcata tggtattgat
    77161 gacaaagtct accatctagg ccaaaaatca tctgcaaaga tttctgccat cacaaaaaac
    77221 gtagctgaag tgagacgcat gacatttatt aaaggcagtg acttttcaga caaagacttt
    77281 attgaccaaa aacaggtgat ttaccttgaa aaaagcttgt atgagtcgct atttccaaaa
    77341 gacgatggtc ttggaaaatt tgtcgaagtg atgggaaatc ccttcagggt cattggggtg
    77401 tttgaatcaa aagaacaaag tggcctcaca agtgggactg agaaaattgc ttatatcccg
    77461 ctacatcagt ggtataatat taatggtgta gtggatgcga cacctgagat cacgatccag
    77521 acctatcgcg cagatgacct caaacctgtg gcaaagcgtg tcagtgatat gctcaatcaa
    77581 acgatcccta aatcagatta tatgtttggt gtcatgaacc ttaaagaatt tgaacgccaa
    77641 ctagacaacc taaacaaatc taattttgtg ttattagctg gtattgctag catctctctt
    77701 attgtaggtg gtattggtgt gatgaacatt atgctagtat cagttaccga aagaacgaga
    77761 gaaattggga tcaaaaaagc gcttggcgct agacgtaagc ttattttaaa acaattttta
    77821 attgaagcgg tcattctaac cttgctaggt ggtgtgatag gggttatttc agggatggta
    77881 tcaggattga tcatcacaag atcactcgag tatccctata tcctatcctt gttctcagtt
    77941 gtcttgagtt tagcattttg ttgtattata gggattgtct ttggactatt accagccatt
    78001 aaagcgtcta agctagaccc aattgaagca ttgcgatttg aataatcaac caaacaagca
    78061 taaaagtcaa aaaggatgac cctttgatgt catccttagc tgcttgatac actaaacgcc
    78121 aatctagaaa gggaagttat gtttaaaata ttagtagttg aagatgatga taccatcagt
    78181 caagtgattt gtgagttttt aaaagcgaat aactatgatc cagattgtgt atttgatggc
    78241 caagctgcct tagacaagtg gcagacaacg tcttatgatc tgattatttt agacatcatg
    78301 ctcccttcct taagtggctt agaagtgcta aaaacaattc gaaagacatc agatgtccct
    78361 attattatgc taacggcatt agatgacgag tacacacagc ttgtaagctt caaccatttg
    78421 attagtgatt atgtgactaa accattttca ccactcattt taatcaaacg tatcgaaaat
    78481 gtattacgcg tctctactcc agatgaaaag cgccaaatcg gagacttact tgttgatgag
    78541 accgaacata gcgtctattg gcaaggaaca ttggtcaaac taaccaaaaa agaatatgac
    78601 attattgatt atttagcaaa acgtcaccaa aagattgtca ccagagacca attgatggat
    78661 gacatatggg gctattctga actggacacg cgtgtccttg acaaccacat caagaacctt
    78721 cgtaaaaaaa tgacgggtat tcccctcaag acaattacag gcatgggcta tttattggga
    78781 gagcgtgagt gagactaatc aaaaaaacct ttttggtcat caatggctta attattgtag
    78841 tggttacctc tattttgtta gtgctttatt ttgcaatgcc aatttattac accaaagtca
    78901 aagacaaaga agttaagcgt gaatttgacc aaacaagcaa gcaaatcaaa ggcaagacgg
    78961 taaccgagat tagagatatc ctaacaaaaa aaatcaacaa agataatatt tggtacagct
    79021 tagtagacag tgataaccaa ctgctctatc cttccttgca actgttagac ggcgtcagtg
    79081 aaagcaaaga cagtcaaaat gttaatatcg tcaccacctt tgacaactct tattctaatg
    79141 ttaaggtgat gagtcaaaaa gtaaccctac gcgatggaaa aaaaatgaca ttgcttgggc
    79201 aatcttcact tcaacccgta acagatgcta gcaaagttct ccttgacctc tacccgtcac
    79261 tattgatttt ttcagtgact gtagggagta ttgtcgccta cttatatagt aggacttcta
    79321 gccggcgtat ccttagcatg tcacaaaccg ccaaaaaaat ggtcaaccta gaaccaaact
    79381 taacgtgtac cattcacggc aaagatgaaa tagcaatgtt ggctagtgat attaaccgtt
    79441 tgtatgcgag tctttcaacg agcatcaaat ccttgcaaaa agagtatgaa aaagcttctg
    79501 attcagaaag agagaaatct gagtttttac gcatgacatc gcacgagcta aagacaccaa
    79561 tcaccagtgt tatcggaatg attgatggaa tgctctataa tgttggtgac tttgcagatc
    79621 gtgacaaata tttgcgaaaa tgccgagacg tccttgaagg gcaagcccaa ctggttcaat
    79681 ccattttatc tttatctaag attgaaaccc tagcttctca aaatcaagaa ctgttttcac
    79741 taaaaagcag cctagaagaa gaaatggaag tctttctcgt cttatcagaa ctaaaacacc
    79801 tcaaagtgac tatcaatctc gaagaacaat tcgtcaaagc caataaagta tacctactaa
    79861 aggcaattaa aaatattatt gacaatgcct ttcactatac caaaccaggc ggccaagtga
    79921 tgattcaact aaaagacaac caactagtga ttaaaaatga agcagagaca ttattgacac
    79981 aacagcagat gaaacagtta ttccaaccgt tttatcgacc agattatagt cgtaacagga
    80041 aagacggtgg tacaggattg gggctgttta tcacccacca gattcttgat cagcatcatc
    80101 tggcttatcg ctttgttgtt cttgatcaaa gatggatggt atttacgatt gattttccat
    80161 cccatcatga cgattgatcc acactagtag gacgacaaaa gtcatcaatc actaaccaag
    80221 ccttgacctt tacccatcat tatttaggca agtataaaaa agaggagaga aatcatatat
    80281 gaagaaaagg aaattgttag cagtaacact attaagtacc atactcttaa acagtgcagt
    80341 gccattagtt gttgctgaca cctccttgcg taataacaca tcatccactg atcagcctac
    80401 tacagcagat actgatacgg atgacgagag tgaaacagca aaaaaagaca aaaaaagcaa
    80461 ggaaacagcg tcgcagcacg acacccaaaa agaccataag tcatcacaca ctcacccaac
    80521 ccctccttca aatgatacta agcagaccga tcaggcatca tctgaagcta ctgacaaacc
    80581 aaataaagac aaaaacgtca ccaagcaacc agacagcagt gatcaatcca ccccatctcc
    80641 caaagaccag tcgtctcaaa aagagtcaca aaacaaagat ggccgaccta ccccatcacc
    80701 tgatcagcaa aaagatcaga cacctgataa aacaccagaa aaatcagctg ataaaacccc
    80761 tgaaaaagga ccagaaaaag caactgaaaa aaccccagag ccaaatcgtg acgctccaaa
    80821 acccatccaa cctcctttag cagcagcagc tcctgtcttt gcaccgtgga gagaaagtga
    80881 caaagacctg agcaagctaa aaccaagcag tcgctcatca gcggcttacg tgagacactg
    80941 gacaggtgac tctgcctaca ctcacaacct gttgtcacgc cgttatggga tcactgctga
    81001 acagctagat ggttttttga acagtctagg tattcactat gataaagaac gcttaaacgg
    81061 gaagcgttta ttagaatggg aaaaactaac aggactagac gttcgagcta tcgtagctat
    81121 tgcaatggca gaaagctcac taggtactca gggagttgct aaagaaaaag gggccaatat
    81181 gtttggttat ggcgcctttg acttcaaccc aaacaatgcc aaaaaataca gcgatgaagt
    81241 tgctattcgt cacatggtag aagacaccat cattgccaac aaaaaccaaa cctttgaaag
    81301 acaagacctc aaagcaaaaa aatggtcact aggccagttg gataccttga ttgatggtgg
    81361 ggtttacttt acagatacaa gtggcagtgg gcaaagacga gcagatatca tgaccaaact
    81421 agaccaatgg atagatgatc atggaagcac acctgagatt ccagaacatc tcaagataac
    81481 ttccgggaca caatttagcg aagtgcccgt aggttataaa agaagtcagc cacaaaacgt
    81541 tttgacctac aagtcagaga cctacagctt tggccaatgc acttggtacg cctataatcg
    81601 tgtcaaagag ttaggttatc aagtcgacag gtacatgggt aacggtggcg actggcagcg
    81661 caaaccaggc tttgtgacca cccataaacc taaagtgggc tatgtcgtct catttgcacc
    81721 aggccaagca ggagcagatg caacctatgg tcacgttgct gttgtagagc aaatcaaaga
    81781 agatggttct atcttaattt cagagtcaaa tgttatggga ctaggcacca tttcctatcg
    81841 gacgttcaca gctgagcagg ctagtttgtt gacctatgtc gtagggggca aactcccaag
    81901 accataaccc cacaaccaag aagctacctg cctgttgacc aatctcaaaa agagatagcg
    81961 tcaacttaga atgaggttca tcagatgata aagaaagtga ctaccccgag tcaaaaaaca
    82021 aaaaaacgtg ttcgtaatgg ctatctccta aaactaggta cagcctgttt actcttaagt
    82081 atactcagtt atggcattgg tcttttgggc cagcctagca tggaaaatac gtttatggga
    82141 atagcgagtg ttgccatgtt aggaagtgtc tgtttcttta ttatctttgc tctaaaccgt
    82201 atctttgacg cactagagga caatttgcga gattagagta ataggtcaaa taatcttcca
    82261 ataatttaga gcacaagcta agttattgga agtttttttg tcttttgaga gccatgatgc
    82321 catcaaaaag tcaaacactc cttatgatgt gagagtcaca tggctaaaat taaaagcaaa
    82381 atagacccca aagccctgtt ttctcaaaac agccaacgaa aaaagacaag ttaaccagtt
    82441 cataaaaaac atcgtagaaa cgttttcaag agctaatgtt ggagtaaatt gactgaagta
    82501 tgatagaatt tttaatggtt gtaccatgac agtcaaactt aaaaactgaa atcagacaaa
    82561 aacattaaat tgcatgtaaa aaat
//
LOCUS       DAUGHY010000003        58301 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.003, whole genome shotgun sequence.
ACCESSION   DAUGHY010000003 DAUGHY010000000
VERSION     DAUGHY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58301
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.003"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(50..2434)
                     /locus_tag="VH752_000162"
     CDS             complement(50..2434)
                     /locus_tag="VH752_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ8288547.1"
                     /translation="MKQEKLIVKSSPLQELHIATGSSRTAKTWKNITLTWQELVERLE
                     KPTVTQETFAEYQKMSRAEKGQAKDVGGFVGGWLKQGKRKNENVQSRSLVALDADSPS
                     KDFLDRLDLLADYAFVLYSTHSHSKKAAKYRLIIPTDRLMMPDEYEPVARYLANQLGM
                     SNFDDTTYQSVRLMFWPSHSRDADFTFKYNDEAFLSVDEVLDTYPDWHDSSFWPESPT
                     HAVKRQREAKKQGDPLSKKGLIGAFCRNYDIRQAIATFLSEVYEEGTTPDRYTYTDGS
                     TANGLVIYDDVFAYSHHGTDPVGDTLVNAYDLVRIHKFGEQDSEAKDNTPTNKLPSSK
                     AMNAFVSDLPEIKDHLMAEALGDFDEELPVEDDRSWLEIDERGEPEVNSYLLATQIIK
                     EVPIYWDGLEFLRYDAKKGIWLPNAEEYLKSYISTKKLGKITKIRHISETVVAIKAQA
                     FSSEVFTESDLNKIVLANGVYDLRDNNFKTKFDPELHARSSHPVVYDPEATCETFEGF
                     LRETVGAENIDFIFEWFGYNFYREYTIQKMLFIYGSGGTGKSTLINILREMIGADNYS
                     AVTLQYLMQERFAKIGLYRKTANFDTDAKPQYLADGAALKMLTGEDTIHADRKNKEPI
                     NFYNYAKLSFAMNELPPMRDFSGGLKRRMMILEMDKVLTQEVKAKYPLDKIMSEVPGI
                     FNRAMEGLRKALSKRDFSISASMRSSVEKWEKGNDVVAMFLEDECELGEDFKVPVRDV
                     YPAYKFYCQDSGYKPLAKNAFNHRLRELNYENKNVKSDGKQAKNWVGFRLKSEF"
     gene            complement(2439..4361)
                     /locus_tag="VH752_000163"
     CDS             complement(2439..4361)
                     /locus_tag="VH752_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEQ8288548.1"
                     /translation="MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVE
                     CLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCLSRYLGLPYYLDPAQWQCTMVLA
                     QELGLPASLEKCALYLKLAQEKDAAGKNLIKYFSVPCKPTKANGGRTRNLPEHDPGKW
                     QMFINYCMQDVVVEMAIAEKLESVPVHDREWDYYVCDQRINDRGVALDKELVASALYC
                     KDVKMESLSGELKALTGLDNPNSRAQLLPWLKEHGYSANGLTKADVEQELKKAEGELK
                     RVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGRWAGRVVQVQNLARN
                     YIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDGYTFYVSDFSAIEAR
                     VIAWFAGEQWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGP
                     GALKQMGALNMGVKEEELQGLVDDWRRANKKIVQFWKDVQRAAIKAIKSRAPIKLGKI
                     RFRYRKGFLFITLPSGRNLAYARAKVEPGDYGDKIIYEGQGDRAYFTAQETYGGKLVE
                     NIVQATARDILAEALLRIEAAGYDVVFHVHDEAIIEGSGLIIEEVNDLMAQAPEWAEG
                     LPLNSEGYVTKYYMKD"
     gene            complement(4404..4961)
                     /locus_tag="VH752_000164"
     CDS             complement(4404..4961)
                     /locus_tag="VH752_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEQ8288549.1"
                     /translation="MTPSTTKVVTGKVRLSYAALLEPKAFEGQEAKYSTVILIPKTDK
                     VTIKKIKDAQKAAYEAAKDNKLKGVKWERVKTTLRDGDEEMDTEEHPEYAGHMFMSVS
                     SKTRPQIIDRYKNPVDSADEVYSGVYARVSLNAYAYNTAGNKGISCGLNNVQIVAKGD
                     YLGGRSSADADFDEWNEEEDEDDIL"
     gene            complement(4972..5370)
                     /locus_tag="VH752_000165"
     CDS             complement(4972..5370)
                     /locus_tag="VH752_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288550.1"
                     /translation="MKIYKVKVKFRQTCHKKFKGKKYSYFSFEELRVGDLVVVETVYG
                     PSVAKVTEVVDANELFTATSYVISKVDTSLLAGKKELMATALTVKANIDAETAEFAAK
                     YKDAYYLGLFDQYKNQNPELAELLTQLKEL"
     gene            complement(5374..6528)
                     /locus_tag="VH752_000166"
     CDS             complement(5374..6528)
                     /locus_tag="VH752_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEQ8288551.1"
                     /translation="MPVENHALLSASSAHRWLYCPMLPRLEADYPSRDTVYTQEGTSA
                     HELSEIKLMYKSGKITKRKFNTLTKAFKENSDFYNEEMEEMTELYTDIVMEHLNAYED
                     AEIELEKRIDFSDWVPGGFGTSDVVILADGVIEIIDLKYGKGMPVSANQNPQMGLYAL
                     GAYASYDMVYDFDRIKMTIIQPRLDSVSSVDIYVEELLYWADNVVLPMAAQADAGIGD
                     WNLSEKVLQWSPVAAKLVPRAQENWELIDKYDYQEPVYLSDEAVAEILDKASAIKKWV
                     ESVEAYALKEALSGKEVPGYKIVEGRSNRVLTNKEAAATILEKNGFEDIYKPKELLSM
                     GALEKMVGKKRFEDMMAFILDKPQGKPVLVKNSDKRPALNSLEQAIKEFE"
     gene            complement(6528..6827)
                     /locus_tag="VH752_000167"
     CDS             complement(6528..6827)
                     /locus_tag="VH752_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288552.1"
                     /translation="MIEVTFKAESMAGVFDAMREALDMPKTVTKDVVEEKPAPKKKEE
                     SVTLTLADIKKMAKAKVEEGKSKDIKEVLKEMNVAKVGELEEYQFAEFVEKLEAL"
     gene            complement(6915..7118)
                     /locus_tag="VH752_000168"
     CDS             complement(6915..7118)
                     /locus_tag="VH752_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288553.1"
                     /translation="MNYLEYALAYLERELEIIDDEVIEVELPGGDWEFVPNPYYEEGL
                     HDSPHYRSQVAKDILDIKGLLGR"
     gene            complement(7265..7651)
                     /locus_tag="VH752_000169"
     CDS             complement(7265..7651)
                     /locus_tag="VH752_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288554.1"
                     /translation="MMTTFEKIIKNLNRIERELVKPEAERRDSLPILSAQLSAIKQDL
                     KLMLWIELPALNNVEKYEALYQGNDFKFSETFLERQATRKAFWRRLAKETFKEDQDKR
                     NFVKLAEKEFKGATLSWQEFLWGKKK"
     gene            complement(7648..7851)
                     /locus_tag="VH752_000170"
     CDS             complement(7648..7851)
                     /locus_tag="VH752_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288555.1"
                     /translation="MSKWELQKQLLANATDFESTVEALFELNELGEISDEELLLNLRS
                     SYRKYSEEKLCIIDKLIKLGGAE"
     gene            complement(7844..8014)
                     /locus_tag="VH752_000171"
     CDS             complement(7844..8014)
                     /locus_tag="VH752_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288556.1"
                     /translation="MNKLELFLLATTVILAVIARVQHEVIKKHNSPENKRRIFREVAL
                     ENSKKWSAERYV"
     gene            complement(8011..8286)
                     /locus_tag="VH752_000172"
     CDS             complement(8011..8286)
                     /locus_tag="VH752_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEQ8288557.1"
                     /translation="MDSRLLQMLDEFEAGLIDRKIKVMKMINNETEIYPLELNKKQVS
                     KMFGVDPKTFDARFNSHKDFPRIETGGREKYPRDLAIEWYHRNWSIT"
     gene            complement(8348..8563)
                     /locus_tag="VH752_000173"
     CDS             complement(8348..8563)
                     /locus_tag="VH752_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ8288558.1"
                     /translation="MSQQHKKWSALVEQRLKEKGWTRVDLALAAGLRSSGTITDLLTK
                     GKGSVDLKVRVSKILSIREPWELFENE"
     gene            8611..9024
                     /locus_tag="VH752_000174"
     CDS             8611..9024
                     /locus_tag="VH752_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288559.1"
                     /translation="MEKYNFWLTIATAVLPAAISAIISFLASRYKNRSDIESLEKKFN
                     HEMTTIEKNHSNDLERLEKQYQHDFEAMEKNHFRELEKMKEAHTLELENIQKQSEAEV
                     NNTVAQGLMTMLGSAMTPALSKYLEENDVFNQPPK"
     gene            complement(9009..9161)
                     /locus_tag="VH752_000175"
     CDS             complement(9009..9161)
                     /locus_tag="VH752_000175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288560.1"
                     /translation="MDEKNPLEQFEEFCKNNSFEYVKGWVDGMQSVLNILSMELSKKK
                     WIYFGG"
     gene            9436..9837
                     /locus_tag="VH752_000176"
     CDS             9436..9837
                     /locus_tag="VH752_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8288561.1"
                     /translation="MLFYRYFLSSHRKADKSMFSTFERIKELAKSRGISLGLLEEKLG
                     LSRNSIYTIKNKKPSAERLQLIADYFHVSTDYLLGRTDNPKIAQDGHASVAIDLKKDA
                     EETFFFDGHELNDEDIDLITSILETRIKNRK"
     gene            9851..10234
                     /locus_tag="VH752_000177"
     CDS             9851..10234
                     /locus_tag="VH752_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEQ8288562.1"
                     /translation="MMTPETVCQEKGIDLVYFDGRGTNTPGMFNKKHNVIAIDTYLDG
                     IYKHKVIYHELGHREHTASYYKLNKEKAELQADRCMIHHLLKEELSYWDNMEDFNYIQ
                     FMEKYELTSIADEVMIKEELEFLIS"
     gene            10245..10796
                     /locus_tag="VH752_000178"
     CDS             10245..10796
                     /locus_tag="VH752_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288563.1"
                     /translation="MDIAKLKEFAITAAGKAKDGAIKANELRKKASQESKIILPPALN
                     LELGAQTAIRKTIDGKYYIGFYSETPELYEFVSLQFDGSRIIEKTVTKGKTTQKGRSS
                     SVLGGAAIGKVLAPGVGTIIGGMAGGARKKKGTINTTATTTSEEIPGKATVALRNIQT
                     GEVKTLKTKLTQAQYVSVNNFFK"
     gene            10913..11992
                     /locus_tag="VH752_000179"
     CDS             10913..11992
                     /locus_tag="VH752_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ8288564.1"
                     /translation="MAFYRKLGSGWEYRITYRDSQGKKREKSKRGFKTKTLAKVAAQQ
                     AEIDLNTLTADLLDITVLDYNRRWADIYKRPHITAKTWQTYTKNFKHIEHYFGTRKLK
                     SITHTFYQQVLNDFGAKVAQQTLDKFHYQIKGACKMAIRDGIIRDNFADGAIVRSQKP
                     AKEESEKFMEESEYLAFIKVAKSKVKYPSYLTTYIIAVTGLRFAEVQGLTWKDIDFEN
                     GYIDINKTFDYSISQNFGPTKNEQSIRKVPIDKNSLEILRNFKSDYYQDNKLGRVCFG
                     ASNNATNKVIKRVTGRNLTNHSLRHTYASYLIAQGVDLISVSKLLGHENLNITLKVYA
                     HQIESLKEKNDHQVKNIFQNLKFDG"
     gene            complement(12039..12125)
                     /locus_tag="VH752_000180"
     tRNA            complement(12039..12125)
                     /locus_tag="VH752_000180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12089..12091),aa:Ser,seq:gga)
     gene            complement(12190..12825)
                     /gene="trmB"
                     /locus_tag="VH752_000181"
     CDS             complement(12190..12825)
                     /gene="trmB"
                     /locus_tag="VH752_000181"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEQ8288565.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF"
     gene            complement(12825..13616)
                     /locus_tag="VH752_000182"
     CDS             complement(12825..13616)
                     /locus_tag="VH752_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEQ8288566.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            complement(13680..14714)
                     /locus_tag="VH752_000183"
     CDS             complement(13680..14714)
                     /locus_tag="VH752_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8288567.1"
                     /translation="MKALFLKRRQNFQKQQNKYLRYVLNDHFVLVLMFLLGFAMVQYG
                     QLLNHFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAVLLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISTKVKERTYLNPLLKLVKQTPSNLWLSL
                     YARAFLRSSDYLGLFLRLMLLSSLSVFFIHNLYLSVSLALIFNYLVVFQLLSLYYHYD
                     YHYMTSLYPENSRSKKKNVLSFLRGLSFLMLIVNMLCCSSAPKALILIVGMVFIACIY
                     LPYKLKKIID"
     gene            complement(14717..15442)
                     /locus_tag="VH752_000184"
     CDS             complement(14717..15442)
                     /locus_tag="VH752_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8288568.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEAFGDRSASLNDIYLALTKED"
     gene            15514..15933
                     /locus_tag="VH752_000185"
     CDS             15514..15933
                     /locus_tag="VH752_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ8288569.1"
                     /translation="MENCIFCSIIQGDIPSSKVYEDEQVLAFLDISQTTKGHTLVIPK
                     QHVRNLLEMTAETASHLFARIPKITRAIQSATGATAMNIINNNEALAGQTVFHAHVHL
                     VPRYNEEDGISIQYTTHEPDFPVLEKLARQINQEVSS"
     gene            15930..16286
                     /locus_tag="VH752_000186"
     CDS             15930..16286
                     /locus_tag="VH752_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEQ8288570.1"
                     /translation="MKVKTVIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISQDLAHKWRYFNQETQAHLTEIQNRMAKYQEDSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(16397..17677)
                     /locus_tag="VH752_000187"
     CDS             complement(16397..17677)
                     /locus_tag="VH752_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ8288571.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSKKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVSQLKTNATVYE
                     NLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGSSYQSASTDQDSTASSH
                     TPATPSSSSDALAADESSSSGSGSLVPPAINPQT"
     gene            complement(17686..18210)
                     /locus_tag="VH752_000188"
     CDS             complement(17686..18210)
                     /locus_tag="VH752_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8288572.1"
                     /translation="MIQQSLVIEEGVPQDAKAVLSLVTAAAKETDFITGVETILEATP
                     QELSDFLSRSQTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQVEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNIKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDIYHMRKSVSQ"
     gene            complement(18185..18646)
                     /gene="tsaE"
                     /locus_tag="VH752_000189"
     CDS             complement(18185..18646)
                     /gene="tsaE"
                     /locus_tag="VH752_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEQ8288573.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHDSAKSRY"
     gene            complement(18800..20260)
                     /locus_tag="VH752_000190"
     CDS             complement(18800..20260)
                     /locus_tag="VH752_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEQ8288574.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSSVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDVSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFSSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIVVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCYSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGATFLFIVNFIILTIL"
     gene            20524..21336
                     /locus_tag="VH752_000191"
     CDS             20524..21336
                     /locus_tag="VH752_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ8288575.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKTIKAVQDKGHHVIISTG
                     RPYRMALDYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLKYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLMQTHHED
                     KYALAKNMRAFFKDEIEIDSWGGPLNILEISSKNVNKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTSYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
     gene            21688..22680
                     /gene="manX"
                     /locus_tag="VH752_000192"
     CDS             21688..22680
                     /gene="manX"
                     /locus_tag="VH752_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEQ8288576.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPERKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDDLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
     gene            22769..23578
                     /locus_tag="VH752_000193"
     CDS             22769..23578
                     /locus_tag="VH752_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEQ8288577.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAAAEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGVIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            23595..24506
                     /locus_tag="VH752_000194"
     CDS             23595..24506
                     /locus_tag="VH752_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ8288578.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            24620..24979
                     /locus_tag="VH752_000195"
     CDS             24620..24979
                     /locus_tag="VH752_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEQ8288579.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGMGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTAINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            25370..26647
                     /gene="serS"
                     /locus_tag="VH752_000196"
     CDS             25370..26647
                     /gene="serS"
                     /locus_tag="VH752_000196"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEQ8288580.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRTLLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFNFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKITANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGETVISPK"
     gene            complement(26869..27639)
                     /locus_tag="VH752_000197"
     CDS             complement(26869..27639)
                     /locus_tag="VH752_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEQ8288581.1"
                     /translation="MTDVSRVLKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYKMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYQRFRKY"
     gene            complement(27636..28502)
                     /locus_tag="VH752_000198"
     CDS             complement(27636..28502)
                     /locus_tag="VH752_000198"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEQ8288582.1"
                     /translation="MALFRKKDKYIRITPNNSLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKEQKIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            complement(28511..29875)
                     /locus_tag="VH752_000199"
     CDS             complement(28511..29875)
                     /locus_tag="VH752_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEQ8288583.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEMIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAY
                     GNIIHLGERDCSLQRHNQKVLEESPSIAIGNTLRHEMGQAAVRAAKAVAYENAGTIEF
                     LLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIKIAAGQPLAINQEDITITGH
                     AIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSAVYNGYAIPPYYDSMIAKII
                     VHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDKRVIAGDYDTSFLMETFLPH
                     YNKD"
     gene            complement(29907..30329)
                     /gene="fabZ"
                     /locus_tag="VH752_000200"
     CDS             complement(29907..30329)
                     /gene="fabZ"
                     /locus_tag="VH752_000200"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEQ8288584.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            complement(30326..30826)
                     /locus_tag="VH752_000201"
     CDS             complement(30326..30826)
                     /locus_tag="VH752_000201"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEQ8288585.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVPQVQLVPNPTASEASSPASVKDVPVEEQPQAESFVAEGDIVESPLVGVAY
                     LAASPDKPPFVAVGDTVKKGQTLVIIEAMKVMNEVPAPCDGVITEILVSNEDVIEFGQ
                     GLVRIK"
     gene            complement(30828..32060)
                     /gene="fabF"
                     /locus_tag="VH752_000202"
     CDS             complement(30828..32060)
                     /gene="fabF"
                     /locus_tag="VH752_000202"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEQ8288586.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWDNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMIDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQERGATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            complement(32075..32809)
                     /gene="fabG"
                     /locus_tag="VH752_000203"
     CDS             complement(32075..32809)
                     /gene="fabG"
                     /locus_tag="VH752_000203"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEQ8288587.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPIKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            complement(32799..33737)
                     /gene="fabD"
                     /locus_tag="VH752_000204"
     CDS             complement(32799..33737)
                     /gene="fabD"
                     /locus_tag="VH752_000204"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEQ8288588.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RRLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHEVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKIPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            complement(33762..34733)
                     /gene="fabK"
                     /locus_tag="VH752_000205"
     CDS             complement(33762..34733)
                     /gene="fabK"
                     /locus_tag="VH752_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEQ8288589.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAKRWQSVSIEK"
     gene            complement(34935..35159)
                     /locus_tag="VH752_000206"
     CDS             complement(34935..35159)
                     /locus_tag="VH752_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ8288590.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            complement(35220..36194)
                     /locus_tag="VH752_000207"
     CDS             complement(35220..36194)
                     /locus_tag="VH752_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEQ8288591.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLASIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVKI"
     gene            complement(36195..36629)
                     /locus_tag="VH752_000208"
     CDS             complement(36195..36629)
                     /locus_tag="VH752_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ8288592.1"
                     /translation="MEYDKINHYLVDIFNRILVIEEMSLKTSQFNDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            complement(36703..37494)
                     /locus_tag="VH752_000209"
     CDS             complement(36703..37494)
                     /locus_tag="VH752_000209"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEQ8288593.1"
                     /translation="MQFKHIIFDVVDDLAILTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGVDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            complement(37716..38852)
                     /gene="dnaJ"
                     /locus_tag="VH752_000210"
     CDS             complement(37716..38852)
                     /gene="dnaJ"
                     /locus_tag="VH752_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEQ8288594.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTSHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYSLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            complement(39133..40959)
                     /gene="dnaK"
                     /locus_tag="VH752_000211"
     CDS             complement(39133..40959)
                     /gene="dnaK"
                     /locus_tag="VH752_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEQ8288595.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVAEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDARLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAAAAQQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            complement(41140..41712)
                     /gene="grpE"
                     /locus_tag="VH752_000212"
     CDS             complement(41140..41712)
                     /gene="grpE"
                     /locus_tag="VH752_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEQ8288596.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELVNERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            complement(41715..42749)
                     /gene="hrcA"
                     /locus_tag="VH752_000213"
     CDS             complement(41715..42749)
                     /gene="hrcA"
                     /locus_tag="VH752_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEQ8288597.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKAFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDNSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            complement(42884..43453)
                     /locus_tag="VH752_000214"
     CDS             complement(42884..43453)
                     /locus_tag="VH752_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ8288598.1"
                     /translation="MRKRLKFPYFLTLLACFLLLIVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPLAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQAA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQVIESNDLTNYD"
     gene            complement(43450..44193)
                     /locus_tag="VH752_000215"
     CDS             complement(43450..44193)
                     /locus_tag="VH752_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEQ8288599.1"
                     /translation="MKYNSLNYLVQLLIVIVFLGGLYLFIKPEESVTPTQLNKKEIQQ
                     KDIKKTDRLRALPKVSVEDWELVLVNRDHITKEMSPELADINGISVDKRIEQATSDFL
                     AAAQAIDSQEHLISGYRSVDYQTELYQSYIKKEMANDPTLTQEAAEALVQTYSQPPGT
                     SEHHTGLAIDMSTVDTLNASDPSVAKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            complement(44183..44890)
                     /locus_tag="VH752_000216"
     CDS             complement(44183..44890)
                     /locus_tag="VH752_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ8288600.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESIVEIDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFLWLIDPDRDKQYIDNGSVTVVEFDDGQFTIKTIGDM
                     SYRYRGREIIEERSDEI"
     gene            complement(45282..45521)
                     /locus_tag="VH752_000217"
     CDS             complement(45282..45521)
                     /locus_tag="VH752_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ8288601.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIIAGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            complement(45740..45955)
                     /locus_tag="VH752_000218"
     CDS             complement(45740..45955)
                     /locus_tag="VH752_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288602.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTVYKKTIIYAKI
                     RLTGIALEPSGVSDGYLMNHHVCLHYY"
     gene            complement(45952..47391)
                     /gene="gatB"
                     /locus_tag="VH752_000219"
     CDS             complement(45952..47391)
                     /gene="gatB"
                     /locus_tag="VH752_000219"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEQ8288603.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIKIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLTATKVLSDYFETAVS
                     LGGDAKQVSNWLQGEVAQFLNAEGKTIEEIALTPENLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISAPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLRD"
     gene            complement(47391..48857)
                     /gene="gatA"
                     /locus_tag="VH752_000220"
     CDS             complement(47391..48857)
                     /gene="gatA"
                     /locus_tag="VH752_000220"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEQ8288604.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFI
                     TVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASSDVKDATSAPVRIADYTSKIGRDIKGMKIAFPKEYLGEGIDPKIKE
                     TVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIEDFDKVFA
                     DYDLILGPTTPAVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYAEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            complement(48857..49159)
                     /gene="gatC"
                     /locus_tag="VH752_000221"
     CDS             complement(48857..49159)
                     /gene="gatC"
                     /locus_tag="VH752_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEQ8288605.1"
                     /translation="MKISEEEVRHVAKLSKLSFSESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDVAEEGTDRALLFKNVPEKENHFIKVPAILDDGGDA"
     gene            complement(49391..49710)
                     /locus_tag="VH752_000222"
                     /pseudo
     CDS             complement(49391..49710)
                     /locus_tag="VH752_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dikinase"
     gene            complement(50154..50708)
                     /locus_tag="VH752_000223"
     CDS             complement(50154..50708)
                     /locus_tag="VH752_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEQ8288606.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFDQG
                     DYIFFAIDCHDQNDPWHPESKLFAAHNIKGTTGRHLYGPLAEVYSYMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            complement(50855..51637)
                     /gene="codY"
                     /locus_tag="VH752_000224"
     CDS             complement(50855..51637)
                     /gene="codY"
                     /locus_tag="VH752_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEQ8288607.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDNEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKAVAAILGELDGNEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            complement(51855..53069)
                     /locus_tag="VH752_000225"
     CDS             complement(51855..53069)
                     /locus_tag="VH752_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ8288608.1"
                     /translation="MKIIEKSSKLEHVAYDIRGPVLDEANRMIASGEKILRLNTGNPA
                     AFGFEAPDEVIRDLIVNARLSEGYSDSKGIFSARKAIMQYCQLKGFPDVDIEDIYLGN
                     GVSELISMSLQALLDNGDEVLVPMPDYPLWTACVSLGGGKAVHYLCDEEAGWYPDIAD
                     IKSKITSRTKAIVVINPNNPTGALYPKEILEDIVALAREHQLIIFADEIYDRLVMDSK
                     EHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKDHVKGYIEGLNMLANMRL
                     CSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQRNFIYEAINAIPGLSAVKPEAGLYL
                     FPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRGFNWKDPDHFRIVYLPSVEELEDVQ
                     EKITRVLYQYRR"
     gene            53300..53752
                     /locus_tag="VH752_000226"
     CDS             53300..53752
                     /locus_tag="VH752_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEQ8288609.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDDFEKQAQIAGITNTKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
     gene            complement(53875..55263)
                     /locus_tag="VH752_000227"
     CDS             complement(53875..55263)
                     /locus_tag="VH752_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ8288610.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGIAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDVEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAVNKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            55335..56300
                     /locus_tag="VH752_000228"
     CDS             55335..56300
                     /locus_tag="VH752_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEQ8288611.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVVLVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            complement(56649..57353)
                     /locus_tag="VH752_000229"
     CDS             complement(56649..57353)
                     /locus_tag="VH752_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8288612.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVI
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYLLGNVFLFSKRELK"
     gene            complement(57366..58268)
                     /locus_tag="VH752_000230"
     CDS             complement(57366..58268)
                     /locus_tag="VH752_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ8288613.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSDISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLAD
                     AKERIYEVLELIGLEKVSKKKKVKDYSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
BASE COUNT        17611 a        12575 c        10183 g        17932 t
ORIGIN      
        1 ggtaactttt ttctagggtt accaaactct aattccaaaa aagtaacccc taaaactcac
       61 tttttaacct aaagccgacc caatttttgg cttgctttcc atcagattta acgtttttat
      121 tttcataatt tagctctctt agccgatggt taaatgcatt ttttgccaaa ggtttgtagc
      181 ctgaatcctg acaatagaat ttataagctg ggtagacatc cctaacagga actttgaagt
      241 cttcgccaag ttcacactcg tcttcaagga acatagctac gacatcgttg cctttttccc
      301 atttctcgac acttgacctc atgctggcac taatactgaa atctctctta cttaaggcct
      361 ttctaagccc ctccatcgct ctgttaaaga taccaggcac ctcgctcata atcttatcta
      421 gcgggtattt cgccttaact tcctgcgtta aaaccttatc catctcgagg atcatcatgc
      481 ggcgtttaag tcctccgctg aaatctcgca taggtgggag ctcattcatg gcaaaagaca
      541 gcttagcata attgtaaaag ttaatcggct ctttattctt acggtctgcg tgaatagtat
      601 cctcccccgt caacatttta agtgctgcgc catctgctaa gtattgaggt ttagcatcgg
      661 tatcgaagtt agctgtctta cgatataagc cgatttttgc aaagcgttct tgcatcaggt
      721 actgcagtgt cacggctgaa taattatcag cgcctatcat ttcacgtaaa atattaatta
      781 gtgttgattt gccagtgccc ccgctaccat agatgaatag cattttttga atagtgtatt
      841 cacgataaaa gttatagcca aaccactcaa agatgaaatc tatattttca gctccgacgg
      901 tctctctaag aaaaccctca aaggtttcac aggtcgcctc ggggtcatag acaacgggat
      961 ggcttgacct ggcatgcaat tctggatcaa acttagtctt aaagttgtta tccctcaggt
     1021 catagactcc attcgctagc actatcttgt taagatcact ctcggtaaac acttcacttg
     1081 aaaaagcctg tgctttaatc gctacgacgg tttcgctaat gtgcctaatc ttagtaattt
     1141 taccgagttt cttagttgag atataactct tcaaatattc ctcagcgttt ggcaaccaga
     1201 tgcctttttt agcgtcgtag cgtaaaaatt ctaagccatc ccaataaatc ggaacctcct
     1261 taataatctg cgttgctagc aaataactat tgacctccgg ttcgcccctc tcatcaattt
     1321 caagccagct tctgtcatct tcgactggta actcttcatc gaaatcgcct aaagcctccg
     1381 ccattaaatg gtctttaatt tcgggtaagt cagagacaaa agcattcatc gctttacttg
     1441 acggcaactt attagtagga gtattatctt tagcctcgct atcttgctct ccgaatttat
     1501 ggatacgaac aaggtcatag gcatttacaa gcgtatcccc cacgggatct gtcccgtgat
     1561 ggctataagc gaagacatca tcatagataa ctaagccgtt tgcggttgag ccatcagtgt
     1621 aggtgtacct atcaggagtc gttccttctt cataaacctc agataagaat gttgcaatgg
     1681 cctgtctaat gtcatagtta cgacaaaagg ctccaataag tcctttttta ctaagcgggt
     1741 caccttgttt tttagcttca cgctgtcttt taacagcgtg cgtcgggctt tctggccaga
     1801 agcttgagtc atgccagtcc gggtatgtgt caagcacctc atcaacactc agaaaagcct
     1861 cgtcgttata tttaaacgta aagtcggcat ctcttgagtg gctcggccag aacatcaagc
     1921 gtacgctttg ataagtcgtg tcatcaaagt ttgacatgcc tagttggtta gccaaatatc
     1981 tagcgactgg ctcatattca tcaggcatca ttaaacggtc agtagggata ataagacggt
     2041 acttagcagc tttttttgag tggctgtgag tactgtagag tacgaatgca taatctgcaa
     2101 gcaggtctag cctatctaag aaatctttac ttgggctatc tgcgtcaagc gcaaccaagg
     2161 acctactttg aacgttttca tttttccgtt taccttgctt tagccatcca ccgacaaagc
     2221 cccctacgtc ttttgcttgc cctttttctg ctcgagacat cttctggtac tccgcaaacg
     2281 tctcttgggt gactgtaggt ttctctaacc tctcaaccag ctcctgccaa gttagcgtga
     2341 tatttttcca tgtcttagct gttcgcgaac tacctgttgc gatatgaagc tcttgcaggg
     2401 gagaagactt tactattagt ttttcttgct tcatctatct aatccttcat ataatacttt
     2461 gttacatagc cttcgctatt taaaggaaga ccctccgccc attcaggagc ctgagccatc
     2521 aaatcattaa cctcttcgat tatcaggcct gaaccttcga taatagcctc atcatgaacg
     2581 tggaaaacaa catcatagcc tgcagcttca atcctcagaa gcgcttcagc tagaatatcc
     2641 ctggccgtcg cctgaacgat attttcgaca agcttaccgc cgtacgtctc ttgcgctgtg
     2701 aaataggccc tatctccttg gccctcatag atgattttgt ctccatagtc gcctggctca
     2761 accttggctc ttgcataagc taagttccta ccgctaggca atgttataaa gaggaaacct
     2821 ttccggtatc taaatcgtat ttttccaagt tttatcggtg ctctcgattt gatggctttg
     2881 atggcggctc tttgtacgtc tttccaaaat tggacgattt tcttattggc cctgcgccag
     2941 tcatcaacta acccttgaag ctcctcttcc ttgactccca tatttagagc ccccatctgc
     3001 ttaagcgctc caggacctcc ttgatagcca agtgctaact ctgaaatttt gcccttttgg
     3061 cgtagttcct tgtcaatctc ctcaattgga attccgaaca tctgactggc agacgcctcg
     3121 tagattttcc cgtgcgtaga aaacacgtca agcctccact gctctccagc aaaccatgca
     3181 atcaccctag cctcaatcgc cgagaagtca gagacataga aggtatagcc atctttagcc
     3241 acgagtgccg ttcgcacgag ctgctttaaa gtgtcgttaa ggctatcgta taaaatctcc
     3301 acagcatcaa tatcacgctt tttaacatac tctctagcat catctagatc ctttatataa
     3361 ttcctagcta agttctgtac ttggacaact ctgcccgccc atcttcctgt ccggctagcc
     3421 ccgtaaaact gaagtagccc atgaactcgt ccgtctgagc acatagctct ttccatagct
     3481 tcatattttt ttagacttga catcgcggtt tgtagtttaa gttctaaaac tctcttaagc
     3541 tctccttcgg ccttcttaag ttcctgttca acatcagctt tagtcagccc attagccgaa
     3601 taaccgtgtt cttttagcca cggtagcagc tgcgccctgc tattaggatt atcaagtcct
     3661 gttagagcct ttagttcacc agacaaactt tccatcttaa catctttaca atacaaagcc
     3721 gaagcaacta actctttatc aagtgccacg cctctgtcgt tgattctctg gtcgcagacg
     3781 tagtaatccc actcacggtc gtgcacagga actgactcta gtttttcggc aattgccatc
     3841 tcaacaacaa cgtcttgcat acagtagtta ataaacattt gccattttcc aggatcatgc
     3901 tcgggcaggt tcctcgttct tcccccgttg gcttttgtag gcttgcaggg taccgaaaaa
     3961 tatttaatga gatttttacc tgctgcgtct ttttcttgcg ctaattttaa atacagcgca
     4021 catttttcca ggcttgcagg cagacccagc tcttgtgcaa gtaccatcgt acactgccat
     4081 tgtgcgggat ctaaatagta aggtaggccg aggtaacgac tgagacaaac tctttcgaat
     4141 tgggcattaa aggcgtgctt tcggacttta tcatcaaata acatatcttt gatgtcttca
     4201 ggtagagact gccttgtcaa atcaaggcac tctacttctc cgccatctat agagtaagcg
     4261 aaaagtaaaa tctcaaaatc ttcagcgtca gcgtacttgt aaaccccatt tttgatgtca
     4321 ttcgaactat aggtttcaat atcaatattt aaatgtctca tacctctcct ttaaaaatga
     4381 ggagcctcta aagaggccct ctactataaa atgtcgtctt cgtcctcttc ttcgttccac
     4441 tcgtcaaagt ctgcatcagc tgacgaacga ccgccaaggt agtctccttt agcaacaatc
     4501 tgaacgttat tcagaccaca agagataccc ttgttacccg cagtattgta ggcataggca
     4561 tttaatgata ctcgtgcgta tactcctgag taaacttcgt ctgcggagtc aacggggttt
     4621 ttgtaacggt cgataatctg cggcctagtt ttgcttgaca ctgacatgaa catgtgtcca
     4681 gcgtactctg ggtgctcttc agtatccatc tcttcgtcac cgtcacgaag cgttgtctta
     4741 acgcgctccc atttaacccc tttgagttta ttgtccttgg cagcttcata agcagctttc
     4801 tgcgcgtctt taattttctt gattgtgact ttgtctgttt ttggaattaa gataactgtt
     4861 gaatacttag cctcttgacc ttcaaaggct ttaggctcta gtaaagctgc atagcttagg
     4921 cgtacttttc cggttactac tttagttgtg cttggtgtca taattcttct cctataattc
     4981 ttttaattgt gttaataact ctgctaattc tggattttgg tttttgtact gatcaaacag
     5041 gcctaaataa taagcatctt tatatttcgc tgcaaactca gccgtttccg catcaatatt
     5101 tgcttttaca gtcaaggcgg tagccattaa ctctttcttg cccgctaaaa gtgaagtatc
     5161 cactttggaa ataacatagc tggtagctgt aaaaagctca ttggcatcaa caacttctgt
     5221 taccttggct acgctagggc cataaacagt ttcgacaacc acaaggtcac ctacccgcaa
     5281 ctcttcaaaa ctaaagtagc tgtatttctt acccttgaat tttttatggc atgtttgtct
     5341 aaacttaact tttactttat aaatcttcat attctattca aactccttta ttgcttgttc
     5401 taagctattt aacgcgggtc gcttgtcgct atttttgaca aggacaggtt taccttgtgg
     5461 tttatcaagg ataaaggcca tcatgtcctc aaaacgcttc ttaccgacca tcttctctag
     5521 agcccccatg gacagtagct cttttggttt gtagatatct tcaaagccgt ttttctcaag
     5581 tattgtcgca gctgcctcct tattagtaag cacacgatta cttctacctt cgacaatctt
     5641 atagcctgga acttcttttc ctgagagtgc ttctttcagg gcataggctt caactgactc
     5701 aacccacttc ttgatagctg aggctttgtc aaggatctct gcgacggctt catcagataa
     5761 atagacaggc tcctgatagt catatttatc aattaattcc cagttttctt gcgctcttgg
     5821 caccaattta gctgcgacag gagaccactg caatactttt tcacttaggt tccagtcgcc
     5881 aatacctgcg tctgcttgag cggccatagg caagacaaca ttatccgccc aatacagaag
     5941 ctcctctacg taaatgtcaa cagaactaac cgaatctaaa cgcggctgaa taattgtcat
     6001 cttgatacgg tcaaagtcat aaaccatatc gtaggaagcg taagctccca gagcgtacaa
     6061 ccccatctgc gggttttggt tagcagacac aggcataccc ttgccatact taagatcaat
     6121 aatttcaatg accccatccg ccaaaatgac gacatccgaa gtcccaaaac cgccaggcac
     6181 ccagtcgcta aaatcaatcc gtttttcaag ttcaatttcg gcatcctcat aagcatttag
     6241 gtgctccata acaatatctg tgtaaagttc cgtcatctct tccatctctt cgttgtagaa
     6301 gtctgagttt tccttaaaag ccttggttaa cgtgttaaat ttacgcttgg tgattttacc
     6361 agacttgtac atcagtttga tttcagaaag ctcatgggcg cttgtgccct cttgagtgta
     6421 tacggtatcg cggctagggt agtctgcttc taatctcggc agcatagggc aataaagcca
     6481 tctgtgagca ctagaagcag acagtagtgc gtgattttct actggcatta gagggcctcc
     6541 aacttctcaa caaactcagc aaactggtat tcttcaagtt caccaacttt tgcgacgttc
     6601 atctctttca agacttcctt gatgtccttt gacttccctt cttcaacctt ggctttagcc
     6661 attttcttaa tatcagctaa tgtcaaagtt acagactctt ctttcttttt aggagctggt
     6721 ttttcttcaa caacatcctt ggttactgtc ttcggcatat ccagagcttc acgcatagca
     6781 tcgaaaacac ctgccatgct ctctgcttta aaagttactt caatcattgt gtttctctct
     6841 ttctgtgtta taatttaatt gtgtataatt gttgacggtt tcctaagccg tcttttttga
     6901 tgcaatcaat agcctcacct ccccaaaagt cctttaatat caagaatgtc tttagcaacc
     6961 tgactacgat agtgtgggct atcatgtaaa ccttcctcgt agtaggggtt aggcacaaac
     7021 tcccaatctc cgcccggcaa ctcaacttcg ataacttcgt cgtcaataat ttctagctca
     7081 cgttcaagat aagctagtgc atattctaaa tagttcattt tctctcctca attacttgtt
     7141 ttaaagctgt taatataaaa actgcgtccg ccagggcttg gctttgggaa caagagagcc
     7201 cccccctggc gtaaaatatc atttaaacca gctagggttt catcaataaa ttttagtgat
     7261 atgttcattt ctttttaccc cacaaaaatt cttgccatga caaagttgca cctttaaatt
     7321 ctttctcagc caacttaaca aagttcctct tatcctggtc ttctttgaaa gtttctttag
     7381 ctaatcgtct ccaaaaggct tttctcgtcg cttgacgctc taagaaagtt tcactgaatt
     7441 taaaatcatt tccttgataa agagcttcat atttttctac gttatttaac gccggaagtt
     7501 caatccaaag catcaatttt aaatcttgct tgatagcact caactgagca gataggatag
     7561 gtaagctatc tcgtctttca gcttcgggct taactaattc cctctcaatc cgattaaggt
     7621 tcttgataat cttttcaaaa gttgtcatca ttctgcgcct ccaagcttaa tgagtttgtc
     7681 aataatacaa agtttttcct ctgagtattt cctgtagcta cttctcaaat ttaacaagag
     7741 ctcctcgtcc gagatttcac ccaattcatt taactcaaac agagcttcta ctgtactctc
     7801 aaaatctgtc gcattagcca acagttgctt ttgtaattcc catttagaca taacgttctg
     7861 cactccattt cttgctgttt tctaaagcca cttccctaaa aattctgcgt ttattctctg
     7921 gcgaattgtg ttttttgatg acttcgtgtt gcaccctagc aatgaccgct aagatgacgg
     7981 ttgttgctaa taaaaatagt tctaatttgt tcatgttata ctccaatttc tgtgatacca
     8041 ctcgatggct aaatctcgtg ggtatttttc ccgacccccc gtttcaatac gcgggaaatc
     8101 tttgtggctg ttaaatcttg cgtcaaatgt tttagggtca accccaaaca tcttactgac
     8161 ttgcttttta ttgagttcta gcggataaat ttccgtctca ttgttaatca ttttcatgac
     8221 tttgattttg cgatcaataa ggcccgcttc aaactcatct agcatttgta ataatctgct
     8281 atccatgata aaattccttt aatatgtttt agctaagtca ctgttccagc agtggctttt
     8341 ttgtgtacta ttcattttcg aacaactccc acggctcacg gatactaaga atttttgaaa
     8401 ccctaacctt aaggtcaacg cttccttttc ctttagtaag taagtcagta atcgttcctg
     8461 aactccgcaa accagccgca agtgccaagt cgactcttgt ccaacctttt tcttttaatc
     8521 gctgttcaac tagagctgac cattttttgt gttgttgact catctgctct cctttctgat
     8581 ataatataaa taaaaatgtt gaggtttatt atggaaaaat ataatttctg gttaactatt
     8641 gcaacagctg ttttgccagc tgcaatttct gctattatta gcttcttagc ttcaagatat
     8701 aagaacagat ctgatattga atcgttagaa aaaaagttca atcatgagat gactactatc
     8761 gaaaaaaatc atagtaatga cctagaaagg cttgaaaaac agtatcaaca tgattttgaa
     8821 gctatggaaa aaaatcattt tcgagaactt gagaaaatga aagaagctca cactttagag
     8881 ttagaaaata ttcaaaagca gtcagaagca gaagtaaaca atactgttgc tcaaggacta
     8941 atgaccatgt taggctcagc aatgacacca gcgctctcta aatatttaga agaaaacgat
     9001 gtattcaatc aacctccgaa gtagatccat ttttttttag ataattccat tgagagaata
     9061 tttaaaactg attgcatgcc atcaacccag cctttaacat actcaaatga attattcttg
     9121 caaaactctt caaactgttc gagtggattt ttttcatcca tactactctc tcctttctac
     9181 tcccaatcgg gagttttttt attaaaagta aagcgaaaat aatcgcgaag tttttcaact
     9241 ttagtattga cattttacaa actatattct aaaatcaagg tatagtaaaa acaccaacaa
     9301 aaacattatt aaactactta taaactcgcc aaagttatat ttttttagtt ttaattttcg
     9361 ttttttgttt cgcttttata ttcgctttac aaattatatt ctagactaaa gtttgtaatc
     9421 agtcaagtag tttacatgct tttttataga tattttttgt catctcatag aaaggctgac
     9481 aaatcaatgt tttcaacatt cgaaagaatt aaagaacttg caaaatcaag aggtatttcc
     9541 ttgggattgc ttgaggaaaa attgggattg agtagaaatt caatctatac aattaaaaat
     9601 aaaaagcctt cagcagaaag actacaacta atcgctgact actttcacgt atcaaccgat
     9661 tacctattag gtcgaactga taatcctaag attgcgcaag atgggcacgc ttcggtcgca
     9721 attgatctaa aaaaagatgc agaagagact ttcttcttcg acggtcacga actcaacgac
     9781 gaggatatag accttatcac atctatattg gaaacgcgca tcaaaaatag aaaatagaga
     9841 ggactgctct atgatgacac cagaaacagt ctgtcaggaa aaaggaatcg atttagtgta
     9901 ctttgacggt aggggtacaa acacccctgg aatgtttaat aaaaaacaca acgtcattgc
     9961 gattgacacc tatcttgacg gtatatataa acacaaagtc atctatcatg aactaggaca
    10021 tagagaacat actgcgagtt attacaaact aaacaaagaa aaagcagagc tacaagcaga
    10081 tagatgtatg atacaccatc tcttaaaaga agagctatcc tattgggata atatggagga
    10141 tttcaactac atccaattca tggaaaagta tgaactgacc tcaatcgctg acgaagtgat
    10201 gatcaaagaa gaattagagt ttttaattag ttaaggagac aagcatggat atagctaagt
    10261 taaaagaatt tgccataaca gctgcaggga aagctaaaga cggtgcaatt aaagctaacg
    10321 aactaagaaa aaaagcctca caagagagca aaataatcct accccctgcc ctaaatttag
    10381 aactaggagc acaaacggct atacgaaaaa ccatagatgg aaaatattat atcggttttt
    10441 attctgaaac tcctgagctt tatgagtttg ttagccttca atttgatggg tcacgaatta
    10501 ttgaaaaaac agtaacaaaa gggaaaacta ctcaaaaagg gcgttctagt agtgttctag
    10561 gaggggcagc aataggtaaa gttttggcac caggcgttgg aaccattatc ggcggaatgg
    10621 ctggtggggc tagaaagaaa aaaggaacca ttaacactac tgccacaacc acttctgaag
    10681 aaatacctgg aaaagcaacg gtagccttaa gaaatataca aaccggagaa gttaaaacct
    10741 taaaaactaa gcttacgcag gctcagtatg taagtgttaa taatttcttt aaataaaaaa
    10801 aaccccacgc tcaaatttgg cgaggagagc gtgaggcaaa accgtataag aaacaaccat
    10861 taaaaaggtc gttttcttgt acccatttta acagaaaaat gaggtgaaaa caatggcatt
    10921 ttatcgaaaa ttaggttctg ggtgggagta tcgcatcacc tatcgtgaca gtcaaggtaa
    10981 aaaaagagaa aaatcaaaac gtggatttaa aacaaaaaca cttgcaaaag tcgctgctca
    11041 gcaagcagaa attgatttaa atacgctgac cgcagacctt ttagatatta ctgttttaga
    11101 ctacaacaga cgttgggcag acatatataa aaggcctcac attaccgcaa agacatggca
    11161 gacatacacc aaaaatttta agcacatcga acattatttt gggactcgga agctcaagag
    11221 tatcacacac accttttatc agcaagtcct taatgacttt ggcgcaaaag tagcccaaca
    11281 aactctggat aaatttcatt atcaaatcaa gggtgcttgt aaaatggcca ttcgtgatgg
    11341 cattattagg gataattttg ccgatggggc cattgttaga tcgcaaaaac ccgctaaaga
    11401 agagtcagag aaattcatgg aagaatcaga atacctcgcc tttatcaagg ttgccaaaag
    11461 caaagtcaag tacccatcat acctgacaac gtatatcata gccgtcaccg gtttacgatt
    11521 tgcagaggtt caagggttga cttggaaaga cattgatttt gagaatggct atatcgatat
    11581 taataaaact tttgattatt caatttctca aaatttcggt ccgaccaaaa acgagcagtc
    11641 catcaggaag gtaccgattg ataaaaatag ccttgagata ctaagaaatt ttaagagcga
    11701 ctattatcag gataataaac tcggtcgtgt atgctttgga gcgtcaaata atgcaacgaa
    11761 caaagttata aaaagagtga ctggcagaaa tctaaccaat cactcgctaa ggcatactta
    11821 cgcttcttat ttgatagcgc agggcgttga tttaatatca gtatccaagc tgttggggca
    11881 cgaaaatctc aacatcacac tcaaagttta cgctcatcag atagaaagtt taaaagaaaa
    11941 aaatgaccat caggtcaaaa acatttttca aaatctcaaa tttgacgggt gaaagacggg
    12001 cgaagacccc gaaacccttg atatatcaag aggataaaag gagaggaggg gattcgaacc
    12061 cccgagcccc gttaaggact acacgctttc caagcgtgcg cactaagcca ctatgcgacc
    12121 tctccattac taaagaagtt aatactgtat caactccttt atgttatcat attttgaaac
    12181 aaaatactac tagaaatttg cctcaactcg gtaaatgact tgacctttat tagagaactt
    12241 ctcttcatat tctgtcatga catttccttc atagttacta gcgtgtaagt caagccagat
    12301 ctgtctcaag gtcatgccat attgagaaaa actagctaaa ctgtactcaa acaagccccg
    12361 attatcggtt ttaaaatgaa tttcaccgtg ttctggcaag atacgcttgt aagtgtctaa
    12421 gaaatccttg taagtcaaac gacgtttttc atgcttggtt tttggccaag gatctgaaaa
    12481 gttgagatac atcatatcca cctcaccatc ttcaaaatag ttggttaaac ttgatccatc
    12541 cactcgcaga agtttgacat tgggtacttc tgaagctaat accttatcca aggcgtagct
    12601 taggacagat aattgaatat caatgccaat gtagttaata tcaggatttt tcaaggccat
    12661 ccctgtgata aatccgccct ttcctgaacc aacttcgata tgaataggac ggtcatttcc
    12721 aaaaacgtca tgccaacggc ccttggcatc ttctggattt aatatcacat agtgtgggtt
    12781 attagctaag tgctcctcag caccttttcg ttttctaact cgcattattt ctttctaaat
    12841 atttctctaa attttctgag ggcataaatc tcctgattca catgctccat gtcacgcttg
    12901 tcaaaacact tgagaatttg tgtcaggtga gaaaattgac cataccaaat aattttttgc
    12961 ataaccttgt cattattttt atagccataa taagacagcc attctgacca acgagaccgt
    13021 ggaatatagt ggctcaacag gtaagcaaca tcatacatcc gatcagtcag acgaacagaa
    13081 tcccaatcta ctaaaaaaat cataccacta gtagtaatca cccaattgct atgtttaata
    13141 tctccatgca caatcgttgc tacttctgat ttgaactctg gtaagctccg ttttaattct
    13201 ttaacgatag cttgtaaata tgaattttgc tgaatttgca agggtgcatt ttgctcaaaa
    13261 tcaaccaata aatcgtatgg gttttcaatc ttataattga gctgaagcag ttgattgact
    13321 aattttttag atttgtgaag gcgcaataga atatgaatga tttgcttact gttcatatct
    13381 tctttggtca atgtacggcc gttaagccat tcttgggcac tcatcatatc accattgccc
    13441 atgcgtttgg cccaaagtaa ctgtggcgca atctgttctt ttgctaaggc aggtagaata
    13501 ggggtcgtat ttaattttat aaagacacat tccccatttg gataagtgcc tttataagct
    13561 ttgccacttt tcccacgtaa gggagtcaag gtaagttctt gttccgtcgt tgtcacctta
    13621 tcgccctcct tttctatctt ttatagtcgt ttacccctat tttacttgtt ttaggctact
    13681 tagtcaatga tcttctttaa cttataaggt agatatatac aagctatgaa aaccatacct
    13741 acaatcaaaa tgagtgcttt tggcgctgat gagcaacaca gcatattgac gattaacatt
    13801 aaaaagctta aacctcgtaa aaaacttaac acatttttct tcttgctccg actattttca
    13861 ggataaagac tagtcatata atggtaatcg tagtgataat aaagtgaaag taattggaat
    13921 acaactaaat agttaaaaat gagtgctaaa cttactgaca aataaagatt atgaataaaa
    13981 aatacagata atgagcttaa tagcattagt cgtaaaaata agcccaaata atctgaacta
    14041 cgtaaaaagg ctctagcata aagggacagc caaaggtttg aaggagtttg cttaactaat
    14101 tttaaaagag gatttagata agttctttct ttcacttttg tagaaattcc tttgactgtt
    14161 gtaaaaagag aataaaactt taaaatactt tgcttgcgat tgctttcaaa tgcgacagct
    14221 tttgcccaat ctaaacgatt tcctctcaaa aaatatcttg tctttcgact aagcactact
    14281 ctttttagtg ccaataatat caaaactaag ataataaaaa taaataaaga caaacctaga
    14341 cgtctaaaaa taggatataa aaagagcaat acagcagtct gcaaagttcc ccaaagcaaa
    14401 aaagagagtc gctcagcttg tttgatataa gaaatcactt cttcttcttt tggaagtaag
    14461 aaatgctgat ctgcctcttc tagatacgtt gctatagatc ccatactgag caataatggg
    14521 atcaatatcc caagacaaac ctgaataggt agatgattcg ttggaaaatg gtttagtagc
    14581 tgaccatact gcaccatagc aaagcctaat aaaaacatca acaccaaaac aaagtgatca
    14641 tttaagacgt aacgaaggta tttgttttgt tgtttttgaa agttttgacg gcgtttcaga
    14701 aataatgctt tcatacttaa tcctcttttg ttaatgcaag atagatatca tttaagctcg
    14761 ctgaacggtc accaaaagct tcttgcaagt cagcaagagt tccttgggca cgaacttgtc
    14821 catgatgcaa aatcacaaaa cgatcacaca ttctttcagc agagtccaaa acatgtgtac
    14881 tcatcaaaat agattttcct tttgcttttt cgacttctag cgtttggatt aagtctgaaa
    14941 tagctaaagg gtctaaccct aagaatggtt catcaagaat aaatagactt ggatcaatga
    15001 caaaggcaca aataatcatg actttttgtt tcatcccttt agaaaaattg acaggaaacc
    15061 attcaagttt gtcagttaaa cgaaacattt ccaagaaagg ttgtgctcgt ttttgtgcca
    15121 cttctaagtc gatatcataa gccatagcta cggtgttgat atgctctgat aaggtcaatt
    15181 cttcatagag actaggagtt tctgggataa aacctatttt ttgacggtag gcaactgcat
    15241 tttctgctag agttaaacca tcaatagaaa tacttccttg ataaggcttc aaaaagccaa
    15301 taatttcgtt gatagttgtt gactttccag caccatttag cccgatcaaa ccgactaact
    15361 ctccattgtc tacagaaaat gagacatcgt ttaaaacagg gatattgtga tatcctcctg
    15421 ttagattttt aatgtttaac atataatttc tttctagttt ttgatatact tataatatat
    15481 tataacaaat tcaagtagag aaggtgatat aaaatggaaa attgtatttt ttgcagcatc
    15541 attcaagggg atatcccctc ttcgaaagtc tacgaagatg agcaggtcct cgccttttta
    15601 gatatctcac aaactacaaa agggcatact ctagtcattc ctaagcaaca tgttcgtaac
    15661 ttactagaga tgactgctga gacagctagt catctctttg ctcgtattcc taaaattact
    15721 cgtgctatcc agtcagctac tggcgctact gccatgaata tcattaacaa taacgaagcc
    15781 ttagcgggac aaactgtttt ccatgctcat gttcatctcg ttcctcgtta taatgaagaa
    15841 gacggtatct caattcaata caccactcat gagcctgact ttcctgtctt ggaaaagctc
    15901 gcccgacaaa tcaatcagga ggttagttca tgaaagttaa aaccgtgata gccgtcggat
    15961 tattatcttt taccgcttac aaagcatatc aaaaacgttg taccataaaa gagctgttat
    16021 ccatcagtcg acaagcaaag gatgctgcac aacttgattt agataacatt aaagcaaacc
    16081 ttgacctcat tcacagtcaa ggaaaagtaa ttcaaaatat tagtcaggac ttggcccata
    16141 aatggcgcta ctttaaccaa gagacacaag cacacctcac tgaaatccaa aatcgtatgg
    16201 ccaaatatca agaagatagt gagctaattt ctagtgaatc acctacttgc caaaccgtca
    16261 ttttaactaa caaccattcc agatagtatt atctccctca tatactttat ttgaaatcac
    16321 agacagctat catctttgat acctgtcttt tttatattca aaatcggata ccttaaacat
    16381 aaaaaccctc agaacgttag gtctgagggt tgatagcagg aggaactaat gatccagacc
    16441 ctgatgagct agattcatca gctgctaaag catctgatga agaagacggc gtagctggcg
    16501 tatggcttga agcagtagag tcctggtctg tactagcact ctgataagat gagccagtat
    16561 ctactccgct tgaataatta gcgtaagagc tatgactatc agaataagat ggagcctggc
    16621 ctgaagagtc atagttgttg tttactgtct gagacttagt tgacccatac aaattttcat
    16681 aaacagtagc atttgttttt aattgactaa ccttatgaag tcctaattct gttcggatac
    16741 gattttggat ttctaacaaa tgattagagg tgacaatttg gtatgatcca ccatctgata
    16801 aagtggcatc ttctcctttt agttgataag ttttaatagt tctaagtgcg tcacgatatc
    16861 ctaatagact agggatagtg cgagaagaga tttcgatatt cgtttgcata ttactactta
    16921 cagcagataa aatcttccga taagagctaa tgctatcaag agcaaggatt tttttcaata
    16981 ccttttgaat gacttcacgt tgacgctttt gtcgaccata atctccctca ggatcatcat
    17041 aacgcatacg agcataaacc aaagcttgtt caccgttaat tttgtgtgtt ccaggcgcaa
    17101 cagtagcttg atattcaggt tcgttttcag caatcgagat aggaaaatca aactcatttg
    17161 taactgtaat ccctccaact gcattcacaa gatcaataag gccttgcata ttaatttgaa
    17221 catagttatc aatggtgata ttcaaaagat cttgcacggt cataatagcc atctgagcgc
    17281 cacctgctgc ataagcagca ttaagcttag cttcaacacc attcatttca ttatttttgg
    17341 gtccagataa cgtggttaag gtatctcgtt ctaaactagt catagttgtt ttcttggtct
    17401 ttggattaac cgtaaccaaa atcatcgaat cactgtttcc ttcccacttg gaggcacgct
    17461 ctgaagagcc tgtatcaaca cccatcaaca agatagaaaa agctcttgtt tgtttaatgg
    17521 catcactttt ctttgaagat gtcgaaaaat ctttaaaggt ctttgataat tctcctgttg
    17581 agaaggtata agcactagtt agatagacac ctaatgccaa gacagttgtt aacacaatag
    17641 ctgtgaacat taaaactatt ttttttccaa ttttcatcga ttcacttact gacttacaga
    17701 tttacgcata tgataaatat ctaagtcatc tccatttttt gatttgatgt cgttttttcg
    17761 catactttca atacgaaaac catacttttt atacaggtaa atagccttaa tatttcttac
    17821 ttgaacgtcc aacttaaggc tttcgatata agggttttct tcagcccaat caagagcaat
    17881 ctctaacaat aattgaccga taccataacc acggtaagtc ttagcaacta acataaatac
    17941 atctgcttca acttggcctt gggataatac ttccccagaa agatttaaaa gccctactac
    18001 tttttcatct aatctagcta gcaggcaaaa atcaataaaa gaggtttgac ttcttgataa
    18061 aaagtctgac aattcttgag gagtagcctc taatatagtc tctacgcctg ttataaaatc
    18121 tgtttctttg gcagctgccg tcactaagga aagtacagct tttgcatctt gaggtactcc
    18181 ttcttcaata acgagacttt gctgaatcat gtgacattat ctccagaagc tgacgagagc
    18241 gctcaccatg cgctaacaag tctagttgac gacctttgtc tcgtttagta atggtaattt
    18301 gcaaatagtc ttgtaataat ccctctccta ataattctcc ccactcaata acggtaacac
    18361 cattaccaaa aagaaaatca tctaaatcaa tagagtctgg atcatcacca acacggtaaa
    18421 tatctaagtg ataaagaggt agacgtcctt catattctct aacaatagta taagttgggc
    18481 ttttgatcat ttgactaatc cccatccctt tagcaatacc ttttgccaat gttgttttac
    18541 cggcacctaa gtcgccagac aatacaatca catctccaat agacaaataa gtacctaggg
    18601 tttctccgta agctttaaga gtatattcat tttcactata aaacatagca acattatatc
    18661 agaaaaaaaa catacttcca agcactacct agaagatgac tccaataagg tattcgtaaa
    18721 gtaaaaacgc ttctttatat taaaggttaa aatcaattaa agaagcggca gttagtagca
    18781 aaataagtaa attatttggt tataagatag ttaatatgat gaaatttaca atgaacaaga
    18841 aggttgctcc ccaaataata gggtgaatat cttttgtttt tccctcaaca acttttacta
    18901 gacaatagaa aataaaggca gcggcaatac cataggaaat agagtaacac agcgccataa
    18961 agaaagcggc aaaaaaagct ggaagagcat ctgcaaattt actccaatta acatcaagaa
    19021 aagaagacac catcatgaca cccacaacaa ttaaagctgg agccgtagca gcagctggga
    19081 caatacctac aagcggtaat agcaatattg ataataagaa gcatactgcg gtggagactg
    19141 ctgttagtcc agtacgtcca ccttcagcaa ttcctgctgc tgattcaaca taggtagtcg
    19201 tatttgaagt tccaaccaaa gccccaatag aagtaccgat agcatctgca aaaagcgcac
    19261 gatccatttt tgaactaaag cctatactat tttccaaagc attctcatcg tcttgagaga
    19321 aaatacctgt tcgacgacca gttccgataa aggtaccaat tgtgtcaaaa gtatctgata
    19381 gactaaaagc aaaaatagtc atgaaaacta gcggtaaacg acttgaatcg ctaaaaagag
    19441 aactcaaacc atcgaaagct gcaaggaaag ttgtacctaa ttcagtccaa gcttcaccaa
    19501 tatggctgat cccaaaattg aggttggaca catccactat tcctaaagga atacctacaa
    19561 gagttgttgc gacaatacca attaaaatag cacctctaaa atttcgaatc acaagagctg
    19621 tcgttaaaag taaaccaaaa atagcaagta atacactaga atccgtaaaa ctagaaatcg
    19681 taggtacaac tccaccattg gcgtctacaa ataatagacc atctgcaaat gttttagccg
    19741 atgctttagc cggttcaaca ccatttacca ttactatatt ttcagcagag atagaaaaag
    19801 taatgatatt tgcgttttta aatcctaaat aagctacaaa gacaccaatt cccccaccaa
    19861 tagcatgctg taaactaact ggaatcgcct tgatgatact tttacgaaac ttggttacgg
    19921 taataaaaat attgaataat ccacaaatga aaaccattgc caaggcttct tgccatgaaa
    19981 agcctaaagc aaaaacaact gtataagtga aaaaagcgtt aagtcccatt cctggtgcca
    20041 gcgcataagg cacattggca aataatccca taattaaggt tgatatggct gctgcgataa
    20101 tcgtagccaa aaagacagca ttagagggca tccccgctgc acctaaaata cttgggttaa
    20161 caaacaaaat ataggacatg gcaaaaaagg tcgttaaacc agccataatc tcagttgaga
    20221 cagttgtccc attttcgctt aacttaaaaa acttttccat tgaaatggag tctccttaat
    20281 taaatagtag gtaaaatcaa aaagcgctta tcactagata agtctttttg acacctcatt
    20341 attattgtta ttgttcgtat taaaatgaac aataacacta tgttaccatt tttttgttca
    20401 cttttcaacg tctatacctt catttattca ttaaaaaaac agttatccta tcttttttac
    20461 taatgaatac gcttaactta tcttttctct ttatcttgga gcaaaatttg ctataattta
    20521 attatgacta aaaaattgat tgccattgat ttggatggaa ccttattaca tcatgacaac
    20581 accatctcta cctatactca aaaaaccatc aaagctgtcc aagacaaggg tcaccacgtc
    20641 attatttcaa caggccgtcc ttaccgcatg gcactggact actatctgca gctcaattta
    20701 aagaccccaa tcattacttt taatggggcc ttgacccaca tgcctgaaca aaaatgggcc
    20761 tatgaacata atgtcacttt ggacaaaggc tacctactaa ggttactaaa atatcaggat
    20821 gatttccaga tggattttat tgccagtgag tatcgaaaaa atgtctacat caccatgacc
    20881 aatccagaaa gtatcgaccc gcaactcttt ggtgttgatg aaattactca ggacatggca
    20941 ttagagatta ccaaaatcac tcgaaatcca aacgccttat tgatgcaaac gcatcacgaa
    21001 gacaagtatg ccttagctaa aaacatgcgt gctttcttca aagatgaaat tgaaattgat
    21061 tcttggggag gccctctcaa cattcttgaa atttcgtcta agaatgtcaa taaagcttac
    21121 gctctgaact atctccttgg aatttacaat atggataaaa aggatttaat cgcctttggt
    21181 gatgaacaca atgacactga gatgctagct tttgctggaa ctagttatgc tatgaaaaat
    21241 gctagccctg tgctactgcc ttatgctgat cagcagctta atttttccaa cgaagaagac
    21301 ggtgttgcca aaaaattgga agaacttttc ttgtagtagc aaacgaaacc actatttttc
    21361 agaaaatggt ggttcttctt ctgtcaacag ctatcaaaaa atgaaaatca ttttaataat
    21421 ttcaacattt ttcagcatat ttttgtttta aacagcttga aaacaccttt aatatcaata
    21481 atctgcctat ttaatcctaa attctgaaaa ttttttcatg ttttgtacta tttttccgaa
    21541 agggcttgat tttttagtga aaacgtttta aaatagaaac gttcttgaat ttcgtttaag
    21601 tgaaaaagaa ttgccaaaat ttagttttgg aaggagatta ttcttcttca tttcaacgaa
    21661 aaatatttta aaggaggaag aacaagaatg ggtatcggta ttattattgc cagccatggt
    21721 aaatttgctg aaggtattca tcaatctggt tctatgattt ttggtgaaca agagaaagtt
    21781 caagttgtta ctttcatgcc aaacgaagga ccagatgatt tgtatggaca cttcaacaat
    21841 gctatccaac aatttgatgc tgatgatgaa atccttgtgc tagctgacct ttggagtggt
    21901 tctccattta accaagctag tcgtgtggca ggtgaaaatc ctgagcgcaa aatggcgatc
    21961 atcactggtc tcaacttgcc tatgcttatt caagcttaca ctgaacgtct tatggatgct
    22021 ggtgcaggtg ttgaacaagt tgctgcaaac atcattaagg aatctaagga cggtatcaaa
    22081 gcccttcctg aagaccttaa cccagttgaa gaaactgctg caactgaaaa agttgttaac
    22141 gcacttcaag gagctattcc tgctggaact gtcatcggag atggtaaatt aaagatcaat
    22201 cttgctcgtg ttgacactcg tcttttacac ggtcaagttg caacagcatg gactccagct
    22261 tctaaggctg accgtatcat tgttgcttct gatgaggttg ctcaagatga ccttcgcaaa
    22321 caattgatca aacaggctgc acctggtggt gtcaaagcta acgttgttcc aattagcaaa
    22381 ttgattgaag cttctaaaga tcctcgtttt ggtaacacac acgcacttat tcttttccaa
    22441 acccctcaag atgcccttcg cgcggttgaa ggtggtgttg aaatcaacga attgaacgtt
    22501 ggttcaatgg ctcactcaac tggtaaaaca atggttaaca acgttttatc aatggacaaa
    22561 gaagacgttg ctacttttga aaaattacgc gacctaggtg ttacattcga cgtccgtaaa
    22621 gttcctaacg attctaagaa aaacttattt gagcttatcc aaaaagctaa cattaaataa
    22681 gtttcaaacg ctataaggtt ttaaaacgat gactagctaa cttattagca aaaagtcctc
    22741 gtttactata ctttgaaagg attacaacat gtcagatatt tcgattattt ctgcaatttt
    22801 ggtcgttatc attgccttct tcgctggtct tgaaggtatc ctcgaccaat ttcaaatgca
    22861 ccaaccactt gttgcctgta ctttgattgg acttgtaact ggacaccttg aagctggtgt
    22921 catccttggt ggtacacttc aaatgcttgc tcttggttgg gcaaacatcg gtgctgccgt
    22981 tgcccctgat gctgcccttg cctctgttgc agctgctatc atcatggtca aaagtggcga
    23041 ctttactcaa aaaggaatta cctttgctta ctcaacagct atcccacttg ctgttgctgg
    23101 tcttttcctt acaatgatcg ttcgtacttt gtcaactgcc cttgtacacg ctggtgacaa
    23161 agctgccgct gaaggtaatt tcgcaggtat tgaacgcttc cactttattg cccttcttct
    23221 tcaaggattg cgtatcgctg tcccagcagc tcttcttgtt gctgttccta cttcagccgt
    23281 acaatctgtt ttgaatgcta tgccaaactg gttgaacgaa ggtatgcaaa tcggtggtgc
    23341 gatggttgtt gctgtaggtt atgctatggt tatcaacatg atggctactc gtgaagtatg
    23401 gccattcttc gccctaggtt ttgctcttgc agctattagc caattaaccc ttatcgctat
    23461 gggtgttatc ggtgtagcaa ttgccttcat ctaccttaat ctttctaaaa aaggtggtaa
    23521 cggtggtaat gcagcaggtt cagctgatcc aatcggcgac atcttagaag actactagaa
    23581 aggggtaaga acaaatgact gaacaaatta aattaactaa atcagaccgt caacgtgttt
    23641 ggtggcgttc acaattctta caaggttctt ggaactacga acgtatgcaa aacatgggct
    23701 gggcttatgc acttatccct gctcttaaaa aattgtatac gtctcctgaa gaccgtgcag
    23761 ctgctcttga gcgccacatg gaattcttta acactcaccc atacgttgca gctccaatca
    23821 ttggtgtaac acttgccctt gaagaagaac gcgcaaatgg tacgccaatt gatgacaagg
    23881 ctatccaagg ggttaaaatc ggtatgatgg gacctcttgc tggtatcggt gacccagtat
    23941 tctggtttac ggttcgtcct atccttgggg cacttggtgc ttctttggct tcaacaggta
    24001 acatcgttgg tccacttctg ttcttctttg gatggaacct tatccgtatg gccttcttat
    24061 ggtatactca agaatttggt tacaaagctg gttctgaaat tactaaagac atgtcaggcg
    24121 gtatcctcca agacattact aaaggtgctt caatccttgg aatgtttatc cttgcagtgc
    24181 ttgttcaacg ttgggtatct atcaacttta ctatcgacct tcctggaaaa caattgtcag
    24241 atggtgccta tgtggtcttc ccagatggcg ctgttaaagg tgctgagttg aaaactatcc
    24301 ttgccaacgc tattggcggt atgagccttg ataaagtcca agctcaaacc cttcaaggac
    24361 aattggactc tcttatccca ggtttagctg gattactcct tacattcctt tgcatgtggt
    24421 tattgaagaa aaaagtttct ccaatcgcaa tcatcatcgg cttgtttgca tttggtatcc
    24481 ttgctcacct tgcaggtatt atgtaatcaa ccaatggaag tctgagatga actcaggctt
    24541 cttttttatg ctttatgtca ggaaaatccc acttaaaata tggtataata actacaacta
    24601 cgcaatgaaa ggaactaaaa tggctcaatc tttaaacact agtgtcgaat acaagacaaa
    24661 agctgtctcc taccttggaa tgggaggcaa agttggtcat attttactcg gtgacaaggc
    24721 ccttgaattt tacaacgata aaaatgtgaa tgattatatt caaattcctt ggacagctat
    24781 caatcacatc ggggctaatg tttcaagaaa aaaagttagc cgccactttg aaatttttac
    24841 ggaccaaggc aagtttttat ttgcttctgg agactctgga aaaatcttaa aaatcactcg
    24901 tcaacatatt ggtaatgaga aagtgattac attaccaacc ttgatgcaaa cctttattaa
    24961 caaatttttt cgacattaaa aaaataatgc gcagtgccga tttatcattt caagaaaaca
    25021 aagaatagta gctctaaatt gctatttagt ggaaaagatt ccctcatttg agtttacttt
    25081 ttcttgtttt ttgttataat agtcctaacg gataagtaat accttttttc attcagagag
    25141 tctgtggatg ctgtgaacag atagggaaaa tggtatgaaa ttctaccgtg tcatcaataa
    25201 aatagagatt aagtgcacag attgtgaagc tggatggaac cgcgccaaag cgctccagca
    25261 tcttcataag ctgtgctttt tctatcgtca gagaatcaca agatccaact gtcttactta
    25321 gccttgtttc actgacagct actaataact atcttaaagg agcttctgta tgctagatct
    25381 taaacgtatc cgtactgatt ttgacactgt cgctgccaaa ttaaaaaacc gtggtgtttc
    25441 tgaagacacc ctgacccatc ttaaagaact tgatgaaaaa cgtcgtactc tattagtgca
    25501 atcagaagaa ttaaaagcag agcgtaatat tgcttctgct gccatcgctc aagccaaacg
    25561 tcaaaaagaa gatgccactc aacaaattgc tgatatgcaa aaagtctctg cagatatcaa
    25621 aactattgac aatcaactag tagctatcga ccagcaagtt actgacatca tcactgtttt
    25681 acctaatact cctcacgatt ctgtccctgt tggagctgac gaagaagaca atgtcgaaat
    25741 tcgccgttgg ggaacgcctc gtgactttaa cttcgaagtt aaggctcatt gggatctagg
    25801 cgaagatctt gatattttag actgggaacg tggggcaaag gtaactggtg ctcgtttctt
    25861 attctataaa aatcttggtg ctcgtttgga gcgtgctctt tataacttca tgctggatga
    25921 acatatcaaa gaaggttatc aagaaatcat cacaccttac atggttaacc acgattccat
    25981 gtttggtaca ggacaatatc caaagttcaa agaggacacc tttgaattag cagataccaa
    26041 ctttgtcctg attccaactg ctgaagtgcc tttgaccaat tattaccgtg gggaaatcct
    26101 agacggcaaa gaattgccaa tttactttac tgcaatgagc ccatcttttc gttctgaagc
    26161 gggttctgcg ggtcgtgaca cacgtggcct tatccgccta caccaattcc acaaagttga
    26221 aatggttaaa tttgctaaac cagaagaatc ttaccaagaa ctagaaaaaa taactgctaa
    26281 tgctgaaaat attcttcaaa aacttggttt gccatatcgc gtcatttccc tttgtactgg
    26341 cgacatgggc ttctcagctg ctaaaaccta tgaccttgaa gtctggattc ctgcacaaaa
    26401 cacctaccgt gaaatttcat catgttcaaa tactgaagat ttccaagcac gacgtgctca
    26461 aatccgttac cgtgatgagg ctgacggtaa ggtcaaactt ctccacaccc ttaatgggtc
    26521 aggacttgct gtcggtcgta ccgtagcagc cattttagaa aactaccaaa atgaagatgg
    26581 ttctgttacc atccctgaag tactccgccc ttacatgggt ggcgaaaccg tcatttcacc
    26641 taaataataa attcacccca gagatcatta aagtgatttc tggggtttac tattttaaag
    26701 agccaccaca tcacttgaaa ctcactacat tgccccttta acaacacttt tcttcacact
    26761 agtttatcaa aagtcttatt agtataccca tttccttatt tacaagacta gttaaaaacc
    26821 cttttggaaa ggcctccctt cacgtaggga gactccaaaa ggatgttgtt aatatttacg
    26881 aaagcgttgg tagcgctcat ctaataattg gtctaatggc ttagcttgca aactggttat
    26941 ttgttcgatg aggttagctt tgatgatatc aacgatttca cttgaaaaat aaccatgttc
    27001 tggaataata cggtctacta ttcccatttt gtagagttca cccgctgtga ttttcatcaa
    27061 ttcagcggcc tcggtcgccc ttgaaccatc cttccataaa atagaagcaa agccttctgg
    27121 gctaagaacc gcatacatag tattttcaag catccagacc tgatcggcaa ccgctaaggc
    27181 taatgcacca ccagagcctc cttcaccaat aatgatggcg ataatgggaa ccttgagatc
    27241 actcatttcc atcaaatttt tagcaatggc ctcaccctgt cctcgttctt ccgcaccgac
    27301 acctggatag gctcctgcag tattgataaa cgtaacaacg ggtcgtccaa atttttctgc
    27361 ctgtttcata aggcgcaaag ctttacgata accttctgga ttgggctggc caaaattcct
    27421 tgccaaatta tcctgtaaat tcttaccttt ttgaatacca atgaccgtaa caggttgtcc
    27481 cgccaaataa gctaggccac ctacaatggc accatcatct gaaaaatggc gatcgccatg
    27541 cagttccata aagtcatcga aaataaggtt ggcgtaatcc aaagttgtta aacgcccttg
    27601 atcacgcgct tcttttaaaa ctcttgatac atctgtcatt ggccacctcc atgaaatgct
    27661 actaagtggg caatcttgtc tcgcaactct gttcgtttca caatagcatc cacaaaacca
    27721 tggtcttgta aaaattctgc tttttgaaaa tcatccggca aattctcacg tactgtggtt
    27781 tcaataacac ggcgtccagc aaatccaacc aaggattgag gttctgctaa aataatatcc
    27841 ccttccatgg caaaactagc tgttacccca ccagtagttg gatctgtcaa aatagtgagg
    27901 taaaagaggc ctgcattaga atgtcttttc acagctgcag agaccttggc catttgcata
    27961 aggctcataa tcccctcttg catacgggcg cctcctgaag ccgtaaaaat caccaccggc
    28021 aggttttctt cgatagccaa ttcaaacaat cgggtaattt tttccccaac aacagttccc
    28081 atactagcca taataaagtg agaatccata atagcaagag caatcttttg ctccttaacc
    28141 atagccttcc ctgtcaaaac agcttcatgc agaccagttg tttccttggc tttttgtaat
    28201 ttttcctgat aacctggaaa acgtaacgga tccttggttt caatacttgt aaataattcc
    28261 tggaaagagc cttcatcaac tgttaaggtc agccgctctt gggcagaaat cctaaaattg
    28321 taagaacagg ttgggcaaat ctttgccaat cccaaatctt tcttataaat catgtgctta
    28381 caagctgggc atttcgcaaa gagttcatct ggaacttctg ggacattatg gctgacagat
    28441 cctttgaggg aattattggg agtaatacgg atgtatttat cttttttacg aaataaagcc
    28501 atattttctc ctaatcttta ttgtaatgtg gcaggaacgt ttccatcaag aaagaggtat
    28561 cataatctcc tgcaatcact cgcttatctg aaatcaaatc gagctggaag tctgtattgg
    28621 tgataatgcc ttcaatttcc aattcaacta aggcacgttg cattttcatc aaagcgtcaa
    28681 atcgattgct tccatgaacg atgattttag caatcatgct gtcataataa ggaggaatgg
    28741 catagccatt gtaaactgct gaatcaactc gtagtcctac accaccactt ggcatataca
    28801 agtctgtaat ctttcctgga ctcggtgcaa agttaaaagc agtattttca gcattgatac
    28861 gacattcaat agcatgtccc gtgatagtga tgtcttcttg attaatcgcc aaaggctgac
    28921 ctgctgcaat tttgatttgc tctttgacaa tatccactcc tgtcacaaat tcggtgacag
    28981 gatgctcaac ttggatacgg gtattcattt ccatgaaata gaatttctcg ctgtcttcgt
    29041 ccaacaaaaa ctcaatggtt cccgcatttt cataggctac agctttagct gctctaaccg
    29101 ctgcttgccc catttcgtgt ctgagggtat tgccaatagc aatggatgga ctttcttcca
    29161 agactttttg attatgacgc tgtaacgagc aatcccgttc tcccaagtga atgatgttac
    29221 cgtaagcgtc tcctaaaatt tgcacctcaa tatgtctagc tgggtaaatt actttttcca
    29281 aatacatggc gccatttccg aaagctccca aagcttcttg actggcagca ttgaaagccg
    29341 cctccaaatc tgcttctgtc tccactttac gaatcccttt acctccacca cctgccgatg
    29401 cttttagcat gacaggataa ccaatcttat tggcaatagc taaggcttct tgagcgttat
    29461 aaacttcacc atctgaccct ggaataacgg gcactcctgc cttaatcatt tcagaacgag
    29521 cattgatttt atcacccatt ttatccataa ccgatgcgga tggaccgata aatttaatat
    29581 tcatctcttc acacatggtc gcaaactttg agttttcact taaaaagcca aaccctgggt
    29641 gaatcgcttg ggctcctgtc acaatagctg ctgacaagac ggaattcata ttaagatagg
    29701 attccttgga acgggcagga ccaatacaga tagcctcatc cgccaatatc gtgtgcagag
    29761 cttccttatc cgcttccgaa taaactgcaa ctgtggaaat ccctaattct cgtgcagcac
    29821 gaatgatacg cactgcaatt tcaccacgat tagcaattaa gattttttta aacataaggg
    29881 tttcttcctc cgcagaacaa tcattctcat tgcccacagg caaaggttaa tgttccactt
    29941 gctgctagct tgccatcaac ttctgccctg gcttcaacga cagcaatggt accgcgacgc
    30001 ttgataaagg ttgctgtcat cacaagttga tcaccaggga cgacttgttt tttgaacttc
    30061 actttatcca ttccagcgta aaagaccaac ttgcctttat tttcttcttt tgacaattct
    30121 aagacaccag ctgtttgagc caaagcttcc ataatcagaa cacctggcat cacaggatag
    30181 tgaggaaaat gcccgttaaa gaaaggctca ttaatagtca catttttaat ggcaacgata
    30241 tggtcgtctg acacttctaa cacacgatca accaataaca ttgggtagcg atgaggcaaa
    30301 gccgcttgaa tttctctaat atccatcatt tgatccgcac caatccttgt ccaaattcaa
    30361 tgacatcttc atttgatacc aaaatttctg taatcacacc atcacatggc gctggcactt
    30421 cgttcatcac cttcatggct tcgataatca caagggtttg tccttttttt acagtatctc
    30481 cgactgccac aaatggtggc ttgtcagggc tagctgcaag ataggctaca ccgactaaag
    30541 gactttcaac aatatcacct tctgctacaa aagattctgc ttgtggttgc tcctctacag
    30601 gaacatcctt aactgatgct ggactggacg cttccgaagc agttggattc ggaacaagtt
    30661 gtacctgtgg tacaggcaca gcatgttctt gatttgatgt cgatgcattg aggtgttgtt
    30721 cgtttttact aaagattagc tcaccttcat ttgttttgaa taaaaattca cgtaggcttg
    30781 aggtatcaaa ctgagccatc aaatctttga tttcttgaat attcaaccta ctcctcccaa
    30841 catttgaaag ctaatacagc attatgacca ccaaatccaa aagtatttga aatggcatag
    30901 ttgatagctg tttcttttcc ttctccaaag atgacatttg cttcgatatc ctctgataaa
    30961 gcttgtgtcc cagccgtcat cggaacatag ttatgacgca tagcctcgat agtggcaata
    31021 gcttcaatag cgcctgctgc tccaagaagg tgacccgtaa acgattttgt tgaagacacc
    31081 ggaacatcct taccaagaac ggctacaatt gcttgacttt ctcctttttc attagctggg
    31141 gttgaagttc cgtgcgcatt gacataatta atagcactgg cttcaatacc agcttcttgt
    31201 aaggctaagt ggatggcttt acgagctcct aatccctcag gatttggaga tgtcatatgg
    31261 taggcatcgc aggtatttcc ataacccaca atttctgcta agattgtcgc cccgcgttct
    31321 tgcgcatgtt ctaggctttc taaaacaagc attcctgatc cttctcccat gataaaacca
    31381 ttgcggtctt tatcaaatgg aatagagctt ctgcttggat cttcagttgt tgagagagct
    31441 gttaaacttt ggaagccagc aatggcaaac ttggttatgg ccgcctctga cccaccaact
    31501 atcatcacat cttgagtccc aaatttaatg gcacgaaagg catccccaat cgcatcattt
    31561 gaagaagcgc aggcagtatt aattgattta catactcctt gagcttttaa agacatggcg
    31621 acatttcctg cagccatatt tggtagcgct tttggcaacg tcattggctt cacacgtttt
    31681 ggtccttttt catgtaagcg aatcacttgc tcttcaatct ccgcaattcc accaatacct
    31741 gaagcaacaa taacgccaaa tcgatcggaa tcaatcatct caatatttaa atcagcatga
    31801 ttaatagcct ctttcgctgc gtaaagagca taaagagaat atctatcgaa acggttcaaa
    31861 tcttttttca caaagtattt gtcaaatgga aaatcctgaa tttcagcagc attcttgacc
    31921 gcataatcag tcgtgtcaaa tttagtaata ggaccaatac caatttggcc agctttcaga
    31981 ttgtcccaaa acgtttcagg atcatgcccg attggagatg ttagaccata acctgttacc
    32041 actactcttt taaatgtcat ttcactacgt ctccttattg cattgtcata ccgccatcaa
    32101 tagcgataac ttgtcccgta atataatctt gttccaccaa gaagcttgct aagtgagcaa
    32161 cttcttgagc ttttccaata cgttttattg ggatttgact taagatttgc tcttgcattt
    32221 tttcaggaag gacacctgtc atgtcggact cgataaatcc tggagcaata gcattcacgc
    32281 aaatgttacg agcagcaact tcacgggcaa cagacttggt aaaaccaatc attcctgcct
    32341 tagaagcggc atagtttgct tgcccaatat tgcctgttaa accaaccaca ctagagacat
    32401 taataattgc tccttggcgg gccttgatca ttggttttaa cactgattga gtcatgttaa
    32461 aagcaccggt caaattgatt ttcaagacgc gctcaaaatc ctcttccgtc attttaagca
    32521 tcaatttatc attagtaatg ccagcattgt taactaaaac atcaatcgaa cctaaacttt
    32581 cgatagcttc attgaccata cgtttagctt ctgatgcttc tgaaacatcc ccagagatgg
    32641 taacaactgt aacgccgtag tcagtaaaac tagcaacaag ctcctcagag atagcagagc
    32701 gaccatttaa gacaatatta gctcctaaac tggcgaattg atgagccatt gctaaaccga
    32761 tacctctcgt tgagcctgtg ataaatatat tttttccttt aatttccatg aatgtcctct
    32821 ctatctaaaa ataaacgtaa gcttaccatg ttttcaacac tagtacatag taaattttta
    32881 tcaattttct tcacaaaacc tgtcaaaacc ttacctggtc ctacctcaat gaattgtgtt
    32941 atgccacttt ctactaaagt cgcaacactg tcataaaaac gaacaggctc catgacttga
    33001 cgggctaata gttctgggat acggtctttt tccataatat tagcttcggt attaccaacc
    33061 aaaggaatct tgaagtcact gaagttgtat ctttccaact ctttagccaa caagcggcta
    33121 gctggttcta acaaagcagt gtggaaagga cctgacacgt ttaaagggat taaacgctta
    33181 actccccttt cctttaaaag ttcaactgcc acgttcacag catctgtctg accaccaatt
    33241 acaatttgac taggagtatt atagtttgct ggagcaacca cttcatgttt agcagctatt
    33301 tgacagactt cttcgatgac ttgcacgtct gtattcataa cggcaaccat ctttccagat
    33361 ccttgtggtg ctgcttcctc catcaagcgc cctctcttag ctactaagga tagggtatct
    33421 tcaaaagaga gtgcccctga tgctaccaaa gctgagtact ctcctaagga aagaccagct
    33481 accatatccg gtttaacgtg atgtaagccc aaaacacggt aaatagcaat tgatgatgtc
    33541 aaaatagctg gttgcgtata gctagtttga ttaagtttta actcgtcact atcaatcaaa
    33601 cggcgcaaat cgtatcccaa tacttgacta gcttgatcaa aggtttttct tacaatagca
    33661 aagttatcat aaaaatccct tgccatccct aatttttggg caccttgacc ggcaaataaa
    33721 aaggctgtct ttgtcattat cattatcctt catgtgttta tctacttttc tattgaaaca
    33781 gattgccagc gcttagcttc attttgaata acacgagctg caccataata aatatctttt
    33841 aaaatcgttt cacagctttc ttcttttctc acaagccctg caatttggcc agccataaca
    33901 gatccattga ctacatcgcc ttcaataaca gcatgtcgaa gcgatcctgc tcccatttct
    33961 tcaatatcag tagctgtttt ttgaccaatt aaaaatgctt tttctgcttt agcgtaagct
    34021 gaggtcaatt tatttttaat agaacggaca gggtggccca caacctgcgc agaaatcacc
    34081 gtatcaatat cttttgctgc taagatttta tctttaaaat tttggtgggc attggattct
    34141 ttagcaacaa caaagcgagt tccaatttga acagcctctg ctcctaacat aaatgctgct
    34201 gctgcaccat gaccatcagc tataccacct gccgcaatga caggaatcga aaccgcttca
    34261 acaacttgtc ttactaaaga catagtcgtt aacttgccaa tatgtcctcc agcttccata
    34321 ccctcagcaa tcactgcatc taccccaagc ttttccatac gtttggctag cgcaacgctt
    34381 gggacaacag gaacaacgat tatacccgcc tggtgcagtc tttccatata ctttcctgga
    34441 tttcctgcgc ctgttgttac tactttaaca ccttcttcaa tgaccaaatc aacgatatca
    34501 tcagcaaaag gagataaaag catgatatta accccaaaag gtctatcagt aatagctttg
    34561 acacgatcaa tattagcttt aacgacttct ttgggagcat tgccaccacc tataatgcct
    34621 aaaccaccag cattagaaac tgcacctgct aaatcaccat ccgcgaccca agccattcct
    34681 ccttgaaaaa tggggtaatc aatattaagt aattctgtaa tacgtgtttt cataataact
    34741 ttctataaaa aataaggaga ataatttgat aatcaaacta ttatagtaag aagaaagagc
    34801 gagctagatc ctaaaaagag tctaatccca ccctaatggt ttgattaata cttattgttt
    34861 tttttagtga tgaacatgaa cataacagca gtaagctttt catagacttc tgatgttact
    34921 tgtcactttt atccttattt tgacttttcc tcaacgtaag caacaaggtc accaacagta
    34981 tttaatcctt cttctgtttc aatttgaata tcaaaagcat cttcaatttc tgaaataact
    35041 tggaaaacat caagtgaatc tgcatctaaa tcatcaaatg tagtttctag tgttacttct
    35101 tctgtctcct taccaagttc ttcaacaata atttcttgta ctttttcaaa tactgccatg
    35161 tttatttctc cttaaaaaat ataaatttgt ttttataaat gtgctaggca catgaataac
    35221 tagattttaa caattagact tccccatgtc aaacctccac caaaaccgga aagtaagatt
    35281 ttttgggtac catctaacct aatctgtccc ttttgaacag cctctgacaa gaggattggg
    35341 atactagcag cactagtatt accataacgc atcatgtttt caaggaattt ttcccgagga
    35401 acatcaatct tgcgtgctat tttatcaagt atacgacgat tagcttgatg caataaacaa
    35461 tagtctatgt catcttttgt aatatctgat tgcgccatta gtgtgaggat gctttttgac
    35521 acatccctaa tcgcaaaatc aaatatggca cgtccatcca tctgaatagt cgctatggct
    35581 tttttgccaa ttgagtacgg agatgaaaga ctagtttctc ctgaaattaa actctgacat
    35641 ctcgcaccat ctgtgtgtaa tgtttccgct aaaacatgtt tatctttact ggcttccagt
    35701 agcacgccac cagctccgtc tccaaaaaga acggctgtcg ctcgatcctg ccagtttact
    35761 aacttagata aagtctctgc acctattacc attccgtttt ggtaggcacc tgatgcaata
    35821 agcttatctg ccatagccaa ggcaaagaca aagccactac aagcagcagt catatcaaaa
    35881 gcaaaagcac ttgtagccgc aatagctgct tgaactttcg cagctgttga tggcatagtg
    35941 gcatcagggc tgatagttgc aacaataata aaatcaatag catcagcttt taacccagac
    36001 tgagttaaaa gctgatcagc aacctgaata gctaggtcac tagtcatttc atctctagaa
    36061 atatggcgtt ctgctattcc tgttcgagaa aagatccact catgactagt gtccattatg
    36121 ctagctaaat cattattagt cactaactgc tgtggaacat agtgggctac ctgactgatt
    36181 ttagaaaaaa tcatttaaac caaatcctcc aaaaattgat gtagattccc tagtccacgc
    36241 accaatgcct gcatctcttc atcactcata tcagcaatga catgtccaac catattttta
    36301 tggaactttg catggaggcg gtccaaaagt cgaccccttt ttgttaatga caggtaaaca
    36361 actcgcctat ctgagcgtga tcgcgttctt gcgatataac cttttgcttc taatttattt
    36421 aagctagtcg ttactgtccc taacgttacc attaattctc tggcgatatc acttggagtc
    36481 acctgatcat atttgccgat aatttcaata gtgtgcatct cttttaaaga gacatcatta
    36541 aattgactgg tttttaaact catttcttca attaccaaaa tacggttgaa gatatcaacc
    36601 aagtagtgat tgattttgtc atattccaag caaatacctc ctaacaataa aactttgaca
    36661 gtcaaattat atcagagtta aaagcacttt gcaagcatga gtctacttac cttgaaaatt
    36721 tggtcggcgc ctttctccaa aagctatgac accttcttta aaatcttcct tgaaagctag
    36781 ctcctcttga atagcaagct ctgccttagc ataatcttcc catcctgtga aaaagctttg
    36841 ccagaccaat gatttcattc cagcataaga atttgaagag ccacgtctca atcgctttaa
    36901 aagttgaaga caaactttgt ctagtttatc agattctgcc gtacggtaga caaacccata
    36961 atcgacaccc ttatcagcag taattccttc acctgtcatc acaagatgag ttgcacgatt
    37021 aagtcctact gcacgagtca atagaaataa accaccggca tccggtgcta gtccaacatt
    37081 tacaaaagct tggataaatt ttgtttgagt actagcgata caaaagtcaa cagctaaagc
    37141 tatattaaaa gccgctccag ctacagctcc atcggcgcaa aggatgactg gttttggcaa
    37201 atgcttgatt gcaaaagaaa tttcttgtac cagttcagca atcttgacta gcgactgcac
    37261 attatctttt gcaacagctt cttgcatttc aactaggtca ccaccaactg aaaacacttt
    37321 tccaacggct ttaattagta aaaaacgcac agatgtatcg cgtttaactt ctgctaatgc
    37381 taccagaatc tcttgacaaa ttggaatatt aaaaccgttt gaaacctctg gacggtttaa
    37441 agtcaatatc gcaagatcat ctacaacatc aaagatgatg tgtttaaact gcataacaat
    37501 cctcttttct gataatttat ttgactaaga aattatttct ctttaaaatc atttgataat
    37561 caaatcaatt acctaattat gatatactaa ataaaccatt ttgacaagag aaaaaaacga
    37621 tatctataaa agaaaacgtt ttctctaact gttttgacat aaaaaaaaac gtataacttc
    37681 gggaaaatta tcctgagatt atacgtttga catctttaaa tatcctctaa ggcatctttc
    37741 actttatcaa aaaaaccttt tttctttgga tgtaacattt tttcaccact cgcttctgca
    37801 aatgcttgca aggcttccct ttgagcgtca ttgagtttgg ttggggtcac aatattaacg
    37861 gtaacatgtt gatccccttg acctccacca cggagttttg gtgctccttt gcctttgagg
    37921 cggaaggttt tacctgtttg tgtcccagca gggatagcca tctcaacatc gccatgaacc
    37981 gttggaattt caacagtatc tcctaaggct gcttgagtaa aagaaatatc taagctgtag
    38041 taaatagtgg aaccattgcg ttcaaattgc ttacttggta agacattcaa aataacaaat
    38101 aaatcaccat aaggtccgcc attaaagcca gcttctcctt gaccttgtaa acggatttgc
    38161 tgaccagtct ctacaccagc tggaatctta acagacactt tatgagcttg cttttcatga
    38221 ctagtaccat gacaggtttg acagggttct ttaatttcct taccactacc atgacaaata
    38281 tcgcaggtga cttgtcgacg catcatacca agtggtgttt gggtgtcaat tgtcattaca
    38341 cctgaaccat ggcacttacg acaagttact ggagcggtac caggttttgc acctgagcct
    38401 aaacaagtgc cgcaggttgc ttcgcgatta tagctaactt ctttttcaac accaaataca
    38461 gcctcttcaa aagataaatt aacacgatat tgcaaatcgt caccttgacg aggggcattt
    38521 gggtttcggc ttccaccgcc accaaagaag cttgagaaga tatcttcaaa accaccaaag
    38581 ccacctccgt caaaaccacc aaagccgcct gctccgccac caaagccgcc ttgagcacct
    38641 gcagcgccgt attgatcata agcagcacgc ttttgtgaat cacttaaggt ttcgtaagct
    38701 tcttgaacat ccttatactt ttgctccgct ccagcttctt tattgatatc tggatgatat
    38761 tttttagaca tttttcgata agcttttttg atatcgtcct gagaagcgtc cttagaaacc
    38821 cccagacgat cgtaatattc tgtattgttc ataatgtaag ataccaagcc gtaaaacgac
    38881 tcaatgaaaa ataggaaatc cgacaaagat actctcgtat ctaggaggta tttctctttt
    38941 ttcgcagggt cgtaggcgtg ttcaaatccg caaaagagtc tttacacgct ttagcctgtg
    39001 ttcgaataat cgaacttgta ttcctttctt aaaatatttt gacgtcagca tttttacatt
    39061 ttatttagcc aaaaacagag gtttggtaca acctctgaat tcggatatta atgttactag
    39121 ataactaaat cattactttt ctgtaaattc gccatctaca acatcatcat tatttgctga
    39181 atcattagct tgtgcacctt ctgcaccttg tgctgcttgt tgagctgctg cagcttgctc
    39241 gtacatttta acagccaaag cttgcgcttt ttcatttaat gcttcaagtt tagctttcat
    39301 gtcgtcaagg ttgccagatt cttgcgcagc ttttaactcg tcaagagctg attgcgctgc
    39361 atcgcgttct gtatcaaagc ctttaccttc agtttctttg attgtttttt cagtagcaaa
    39421 gatagcttgg tcaacttcgt ttttaaggtc aacttcttct ttacgtttcg catcggcttc
    39481 ggcattagct tcagcgtctt tcatcatgcg atcaatttct tcttcagaaa gtccgtcgtt
    39541 tgatttgata acgatgtgtt gttccttttg cgtaccaagg tctttagctt ttacagaaac
    39601 aataccgttt ttatcgatat caaatgttac ttcaatttgt gggattccac gaggtgcagc
    39661 tgggatatca gtcaattgga agcgaccaag agtcttgtta tctgctgcca ttgggcgttc
    39721 accttgaaga acatggatat caacagctgg ttggttgtct gctgctgttg agaagacttg
    39781 tgatttagat gttgggatag tggtgttgcg gtcgatcaat ttggtgaaga caccacccat
    39841 tgtttcaata ccaagtgaca atggtgttac gtcaagaagg acaacgtctt tcacatcccc
    39901 agtgataaca ccaccttgga tagcagcacc catagcaacc acttcatcag ggtttacaga
    39961 tttatttggt tctttaccag tttcagcttt tacagcttcg acaactgctg ggatacgagt
    40021 tgatccacca acaaggataa cttcatcaat ttctgacaat gacaatcttg catctgaaag
    40081 ggcttgacga actggagttt tcgtacgttc tacaaggtcg cgagtgagat cgtcaaattt
    40141 agcacgagat aagctcatct ccaagtgaag aggaccggca gaaccagcag tgatgaacgg
    40201 taatgaaatt tgtgtttgtg tcacacctga aagatctttt ttagcttttt cagcagcatc
    40261 tttcaagcgt tgaagtgcca tcttatcttg tgataagtca atgccatttt ctttcttaaa
    40321 ttcagccact aagaaatcaa taattttttg gtcaaagtcg tcaccaccaa gtttgttatc
    40381 acctgctgtt gcaagaacgt cgaagacacc atcacctaat tcaaggattg atacgtcaaa
    40441 tgtaccacca ccaaggtcaa aaactaagat tttttcatcc ttgtcagtct tgtccatacc
    40501 ataagcaagt gcggctgctg ttggttcgtt aacgatacgt tctacttcaa gacctgcaat
    40561 tttaccagcg tctttagttg cctgacgttg tgcatcgttg aagtaagctg gaacagtaat
    40621 aactgctttt tctacttttt ctccaagata gtcttcagca taacctttaa ggtattgaag
    40681 aatcattgct gaaatttctt gaggagtata ttctttacca tttgcagaaa ctttttcaga
    40741 agttcccatc tttgatttga tagagataac agtatctgga tttgttaccg cttgacgttt
    40801 tgcagcatca cccacgataa tttcaccatt tttgaatgat actactgaag gagttgtacg
    40861 attgccttct gggttagcaa tgatttttga ttcagtccct tcaagaactg ctactgctga
    40921 gtttgttgta cctaagtcaa taccaataat tttagacatg ttaatacctc ttattttata
    40981 atactttatt actttttatt tcaatttatc ttagtaagca ctcgaattat tttacttcca
    41041 attctaagca atcactatag tttaatcagc aagttgaata gcttctaact tgctatctag
    41101 tttactgtca ttacggacaa aaacgaaaac tcatttacct tagttataaa caactaccat
    41161 cgctggtctt agcagacgct cgtgcaattt atatcccttt tgaaagactt ctgcaatgct
    41221 atcggctgga tgttcatcat cggctgggag ggtctgaact gccatatgaa agttgtggtc
    41281 aaatgagtcg acttctactt cttctacgcc ttcttctttt aatgcttgaa taaggctatc
    41341 acgagtcatt tctaaacctt ttttaacatc atctgtcaac ccttcaacag ccaaggcgcg
    41401 ttctagattg tcaagcgatg gtaagattgc ttttgccaga tcctgagaac gatagcgttg
    41461 aagttgttga cgttcttcac tagagcgacg ctgtatattc tgcatttctg catgagctcg
    41521 caagtattta ttttcaaact catcagcacg ttcgtttaca agttctaact ctgatttttc
    41581 aggagtttct tcaataactt cctcaactgt ctcttcagtt tcagtggctt ccacttcttc
    41641 ttgtaaatcg tcttttttaa tttcttctga cactgagtgc ctccttttaa ataacttatt
    41701 gaatactgcc attatcagtg aacttcataa tggttagaat ttaggtatcg ataataatca
    41761 cctaatttag cagccaatac ctttccaata atgtttacca aactaacaga cctacgataa
    41821 tccatatcaa taggaccaat taaactcagg agtccaaacc cacggtaagg aatcaaaaat
    41881 ttgtgagtta aaactgacac atctgcaagt gcggcttctt gactatctgc cacttggaca
    41941 gaagccatct caccttcttt cagactttgc ctcaaagaaa ttgctacttg ctgttcatta
    42001 tccaaaaact gatacgttga tagatctgaa tacgttaaag aatttacctt gccagcaaca
    42061 aataccgttt ccaaaaacag ctctgaaaaa acataatcaa atagttgtaa aacattatcc
    42121 gtagtcacaa aatacttttg aacgatctga ggaatttctg tcctcaactt ataatgaata
    42181 tctatgacac tattatccag caagcgctcc tctacgatag ctttaaatgc tatcaagtca
    42241 cgtgtcaaaa agtttcttgg aatagcaaac tgcactgtca ctggcttaga ttcatctaaa
    42301 gtcatgactg ctagagcatc atggttgcta agctgaacaa catcaaaccc tgttaaacgt
    42361 tgacgagctg gctccacatc taaaataacg gatgtatagc ccgtcatctc cgctaaaatg
    42421 tgactagctt tttgaagcat gtcttctaat ttgaatgctt caaaatcaaa agcttttatg
    42481 acatgataaa tatcttgctc atcaatacta tctagcctga gggagtgctc tacaaaatac
    42541 ttaaaaccag ctggacttgg catccgtcca ctagaagtgt gcgctttttc caacaatccc
    42601 aacttctcta gttttgccat atcattacga atagtggcgc tactagaatc aatcgttctc
    42661 tgtaaagctt tagaccctac tggttcatga gtttgtgtaa atagctcaac gattaaattc
    42721 aaaatatcat tttgacgttg tgtaatcaca aaccaaccac tccctttcgt ttagcactct
    42781 atttagtaga ctgctaattt ataattctat tatactcttt atgggagaca tgtcaaaaaa
    42841 aaaactcaaa aaaattagca ctcttttata aagagtgcta atttcagtca tagtttgtta
    42901 aatcgttact ttcaatgacc tgaattagtc gcctagcata ggttttatca gtataatgca
    42961 aatacttttg taaactttta gctggtgctt tatacccttt ttctgtagca agattacgat
    43021 acaaacgctt atcgacttct ttgccctgcc gaagcattcg taaataatct ctaattgcac
    43081 tagcatcatc tttataaata gcatatctca cgttttggtt ggtttgttta ggggacttta
    43141 gggtaatggc tgcttgtcct ggagttgctt tcttgctaaa aagattatga tacttagacg
    43201 ctagtaatgt ttttccatca tgggtttcca aaagaatttg agcaatcaaa atagatggtc
    43261 taatgccata atattttgcc aatggtttaa cttctttacc catttttgta ataaattgtt
    43321 tttggctgta gttcaccctt acttctttat ctgcactagc tatcctttgg ctactcagca
    43381 aaggacaaac aatcagcaat aaaaagcaag ctagaagcgt taaaaaatac ggaaacttta
    43441 gtcgttttct catcgtttct cctttaaagc agcaatgtac tcttccaacg ttaggttgtg
    43501 ctgagccata taacgagcag acgccttacc tacatagcgg taatgccaat cctcataatc
    43561 aacccctgta ctagtctttt ttccttctgg aaagcgcaag acaaacccat aatcaggtgc
    43621 aattttttga actgctttag ctactgatgg atcactggca tttaaagtat ctacagtgct
    43681 catgtcaata gcaagaccag tgtgatgttc actagtccct ggcggctgtg aataggtttg
    43741 caccaaagcc tccgcagctt cttgtgtcaa tgttggatcg ttagccatct cttttttaat
    43801 ataagattgg taaagctctg tttggtagtc tacggaacgg tacccagaga taagatgttc
    43861 ttgcgagtcg atagcttgtg ctgctgctaa aaagtcacta gtggcttgct caatgcgttt
    43921 atccacagaa ataccattaa tgtcagctaa ttcaggactc atttcttttg taatgtgatc
    43981 gcggtttact aaaaccaact cccaatcttc aacagatacc ttaggtaaag ctcgtaaacg
    44041 gtccgttttt ttgatatctt tttgttggat ttctttttta ttcaactgag ttggtgttac
    44101 tgactcttct ggttttataa agaggtaaag ccctcctaaa aaaacaataa caattagcaa
    44161 ttgcactaaa tagtttaacg aattatattt catctgaccg ttcttctatt atttcacgac
    44221 cgcggtagcg gtaactcata tctccaatag tctttatcgt aaattggcca tcatcaaatt
    44281 caacaactgt aacactccca ttatcaatat actgtttgtc tcgatctgga tcaatcaacc
    44341 acagaaacgt tccaattgtc ataccatggc taaccacaag agcatttccg ccgccagcat
    44401 tttgaatgga caaggcaata gcttcaaacc cctcccaaat gcgcttacga agaacttccc
    44461 atggttctgc ccaattagcg gtatctattt ccacgatgct ttctgccagt tcagaatagg
    44521 gaacatcacg taggttatca cgattttcaa aggcttttgt tcttggaagg acacctagaa
    44581 ataattctga atcataagtc ccttctagac tcccaaaaca ccactcacga atacgattat
    44641 ctttagtata aggcaaaaat tcgttttctg attctcttaa aataatttct attgtctgca
    44701 tggttcgacc tgaatcactt gaaaacgcag ccttaaatgg tatagttgca tcttttaaac
    44761 ctaagccaag ttctcgaata ccttcttctc ctttttttgt aagaggtgtg tcactccaac
    44821 cctgagcgcg accaatggta ttaaacatcg tttttccatg tctggcaata tacaaccttg
    44881 tttttgtcat tttaacctcc taaactgtct tattaattat tatagcattt aaagaaaaca
    44941 cttgcaaaac acttcctgat ggctatttga gatcaaatag accactgact tattgtctat
    45001 tttattaaaa acaatgctat tgctacaatt caaatcatca cataaatttc caaacgatta
    45061 catttttgtg tttcgctcaa atgtatataa caacagaccc acactacaac taaactgacg
    45121 ttacaaaatg atgtcaaaac agtaacaaaa tctaactatt tgtcaagaaa acgatattaa
    45181 attttcacac aactatctta taatgaggat gcaaccaata aaaagataag acattttatg
    45241 ttgcaaaaaa ggctgcttgg aaacttccat gcagcctttt tttaatacct ttttccaaaa
    45301 atagctgaaa caagtacaat tacaattgct gctcccaaaa tggacgggaa aatagccata
    45361 cctgccaact gaattcccca atccccaaaa agcctttgcc caacagctga ccctatgaga
    45421 cctgcgataa tattagtaaa acaacccatg gactggcttt tttcagtcat tttacctgct
    45481 atcatgccga taaaggcacc tacaattaaa gaccatatca tcgttaacac ctcctttctt
    45541 gtaaaatcct tttagaagaa ctgctatcca ttaaatgttc ttaactttag ttgattcctt
    45601 taccttcttc tttagtaagt gtttccgtgc cttagtaaaa tagaaaaaac aattccttct
    45661 attaagatag atagaccgag aacgactaga agtcttcaca cactaaataa cttaacaaac
    45721 tttatcacac agctaatcgc taataatagt gtagacaaac gtgatggttc attaaatacc
    45781 cgtcagaaac tcctgaaggt tctaaagcaa tacctgtcag tcttatttta gcatagatga
    45841 tggtcttttt atagacggtc actaacatga catacaaaaa ggcagcctgt gaaagaccga
    45901 ccgccttttt ttcagagaca aaatacttta acttattgct atcagcaact atcaatctcg
    45961 caatttttgc aattcttgtg ctaataattg ttgagcgact tgtggattag cttgaccttt
    46021 ggttgccttc atcaagaagc ctgtgaaagc cttatcagca ttacgtttgc ctgatttgaa
    46081 atcagccact gcagcttcat tatcagcaaa gacttggtga ataattggta ccaaaacagc
    46141 gggagcagaa atctgaacaa gtcctgcctt ttcgacgtag gcgcgagctg agccaccatt
    46201 tttagccaaa tgcacaaaga ccttcttagc cattttagaa gagattgtac catcagcaat
    46261 gatagcaatc atctccacca aattttctgg tgtcagagcg atttcttcaa tggttttgcc
    46321 ttctgcattt aagaattggg caacttctcc ttgcaaccag ttagagactt gcttggcatc
    46381 gccaccaaga cttactgcag tttcaaagta atctgacaag actttggtag ctgttagctg
    46441 gctagcatca taagctgaga gaccaagttc ctcttcgtat ttagcacgac gttgagctgg
    46501 gaattgaggt aattgagcac gcatctcgtc aatccaagca tcatcaatct catacaaggg
    46561 taggtctggt tctgggaaat aacgatagtc cgctgcccct tctttgaccc gcatcaaaat
    46621 agtcccctta ttcgcttcgt catagcggcg cgtttcttgg cgtatcaccc cacctgaacg
    46681 caagagtttg gcttgacgtt ccacctcaaa ttcaagccct ttacggacat ttgagaagga
    46741 gttcaagttt ttcaattcag tctttgttcc aaattgttct tgcccgtaag gacgaaggga
    46801 aatattggca tccacccgca tagagccttc ttccatcttg acgtcagaaa tacccgtata
    46861 ctggatgatt tctttcagag ctgtcaaata agcataggct tcttcaggag aacgcatgtc
    46921 ggcttctgac acaatctcaa tcaaaggaac tccttggcgg ttaaggtcta cataagaata
    46981 accatctgtg ccgtgagtgt ttttaccagc atcttcttcc aagtgtgcgc gttcaatacg
    47041 aattttcttg gttgaaccgt cttccaattt aatcttaatc catccgttgt agccaatcgg
    47101 ctcatcaaac tgtgaaattt gataagcttt aggattatct gggtagaaat aatttttgcg
    47161 gtcaaagtgc atctctttat ggatgtccat attgagggca agcgcagctt ttatgcccgc
    47221 atcaataacg cctttgttca tgactggtaa gacccctgga aaagaccaat caatcacatt
    47281 cgtgttggca tttgggtcct caccaaagtg agctgacgaa ggtgagaaaa ttttggaatt
    47341 ggtatttaat tccacatgga cttcaagccc aataatggtt tcaaaattca ttacttgtca
    47401 cctccaaaaa taatcggttg ttgcttgtga taatctgtca ccgcttcaaa ggctgctgcc
    47461 gcttgataga tggtttcttc agcgtatttc ggaccaatta attgaagccc tactggcaag
    47521 ccatcgacga atcctgctgg aatggaaatt cctggcagac cagccaagtt aactggaatg
    47581 gtcaataagt cggccaaata catggcaaca ggatcgtggt tgagtgtgtc taagccaaaa
    47641 gcaaccgccg gtgttgttgg gcctaaaata agatcataat ccgcaaagac cttgtcaaaa
    47701 tcttcaataa tcaaggtacg cacttgaccg gcctttttga aataagcgtc ataataacct
    47761 gatgacaaac taaaggtccc aagcatgata cgacgtttaa cctcatcacc aaagccttgg
    47821 ctacgagtgt taacatagat ttcatccaag tttttagcat cgtcagcacg gaaaccatag
    47881 cgaatgccat caaaacgttg taagtttgag gacgcttctg acgaagcaat aatgtaatag
    47941 acagccacac catatttgct atgtggaaga ctaacttcct caacagtagc tcctaaggct
    48001 tcaaattgtt tagctgccgc aagaaccgtt tccttaatct taggatcaat cccttcgcca
    48061 agatactctt taggaaaggc aatcttcatg cctttaatgt cacgaccaat cttagatgtg
    48121 tagtctgcaa tgcggacagg cgctgaagta gcatccttga catcgctact tgcaatcaca
    48181 tttaataatt gggcgttttc tttaactgtc ggagcaaaag gtccaatctg atcaagtgaa
    48241 cttccaaaag caatgagacc atagcgagaa acagtgccat aggttggttt caaaccgaca
    48301 acaccattaa atgcagctgg ctgccgaata gaaccaccag tgtctgaacc aagagataaa
    48361 cggacttgtc ctgaagcgac tgctgttgcc gaaccacctg atgagccacc aggtactttt
    48421 gtgtggtccc aagcattttt cgttttcttg aaataagagg tttctgttga accacccatg
    48481 gcaaattcgt ccatgttggt ttttccaatg acaatcatgt ctttagcgta ggcatttgct
    48541 actgatgtgg catcaaaaat cggctcgtag ttatacaaca tttttgaagc ggctgtggtt
    48601 aggatgccct tggttgagat attatcctta acagctagtg gaatacccga catgaggtta
    48661 tcagcatcaa tacctttggc atcgattgcc gcagcttgtt taagagcagc ttcttctgaa
    48721 acagtgataa acgacccgac agcctcctca cgagacttga tatcttcaag tgttttttga
    48781 gtcaattcgg ttgcggaaat ctccttagct acaaggaggt catgcaactc ttcaatggtt
    48841 ttatgattaa aagacattag gcatctcctc cgtcatccaa aatagcaggt accttgataa
    48901 agtgattttc tttttcagga acatttttaa agagcaaggc acggtctgtt ccttcttcag
    48961 cgacatcttg acgcatcaca tttttcttat ctgccatggt tgttgtaatg gcaacacctt
    49021 cggtatcaac ttcgttcagc aattcaacca tgtcaacgat tttagatagg gtcgttgcaa
    49081 aggttgttgt ctcactttct gaaaatgata attttgatag tttcgcgaca tggcgaactt
    49141 cttcttcaga aattttcatg atttctccca tccaagatga caagaagtat tccaattcca
    49201 aagaacagct ctgctattct ccaaaggtag cggaaaaact cctcacatca tttttacata
    49261 gtctcaatca ttatatcata attgcgccca tttgccatag gtgactaatg caattctaga
    49321 aagccatata gcaaaaaact tcctgactaa ggtcagaaaa ccttagtcag gaagtgtgac
    49381 aaactaaact ctatttatct tgagtaagac tgtggcctga agctttgatg gcagcctgag
    49441 cagccgctaa gcgagtaagc ggcactctaa atggcgaaca agaaacgtag gtcaggcctt
    49501 ggctgtggca aaaggcaatg gaagcaggat cgccaccatg ttccccacaa ataccaagtt
    49561 tcaactcagg cttaaccgaa cgggctaaag agacggccaa tccctttctg atcaaaggtc
    49621 tggaacggtt ccttatcaaa aaatacccct gtcaacgtat tcttccaagt attttcctac
    49681 aacatcacag aaaaagtaac gttctttcaa gtcggtaata tgtttacgaa ggtaataaag
    49741 gggtgcttta ttctaattta ccaaatcctc aacgattgtg acaacatgtg atgtgataag
    49801 cctaagataa aaatggtaca gactctagca ttcaagagtt tgaagcttta tttatattct
    49861 aatgctgaaa agactagagt ttgtctaatg gctgtgagac gatcctctaa accatcaact
    49921 caaatcattt ctcaaacagc taaaatcctg tctccttcaa aaaaacatgt gttctatctg
    49981 acacagtcca aagtttagtt gccttttaga cagcacaaga atcatcagga ctaattctga
    50041 ccagcatgtc acatacctca tctacctaac ccatacatac agtaaaacca catagagcaa
    50101 tgattacggt taccaaactc ttagtcactg aatcaatagt ttggtaggct cttttagtaa
    50161 tcgttaataa gcttagcacc aagaacctgc tcaaagtgag ccaaagacca ctcataagat
    50221 tcttttgtaa ggctagcgac agccgattta acaatctcta attgataacc aagatgataa
    50281 gcgtcaatag cagtatgtaa gacacagata tcagataaaa ctcctgttaa cactaattga
    50341 gtgatacctc tttctcgtag tctaatatct aaatcagtcc ccgaaaaagc tgaataataa
    50401 cgcttatcaa tccaaaaaac tctgggatgt tgcttcatat aactgtaaac ttcagctagt
    50461 ggaccataaa gatggcgtcc agtagttcct ttgatattat gtgctgcaaa taacttactc
    50521 tcaggatgcc aaggatcatt ttgatcatga caatcaatag caaaaaaaat atagtctcct
    50581 tgatcaaatg ccgttttagt tacctctgct attttagtgg caatagcctg agcagattta
    50641 cctgcactta atttaccatc atctgctaca aaatcatttg tgtaatcaat tgaaatcaat
    50701 gctctcatca ctctctccta aacagggaaa cctctcccct tatctcttat atatggcatt
    50761 gtatcagttt ttattacagg tatcaagaca tctatttgca tctaccatta acagcaaaaa
    50821 acatctcttt tcaagagatg ttgtcttttt ctatttaaaa ttcttttaat ttagcaaaaa
    50881 taccttcgtt gataactttg aggtatgtcc ctttcatacc aagagaacga ctttcaataa
    50941 tccctgcact ttctaattta cgcagagcat tgacaataac agaacgggta ataccaatac
    51001 gatcagcaat aacagaagct gtcaatcgtc cttcgttacc atctaactct ccaagaattg
    51061 ctgcaacagc tttcatttct gaataagaaa gcgtattaat tgccatgttg accgctgttt
    51121 gtttacggat ggtgtcttct aagttttctg tctgaagatt taatagttga atccccacaa
    51181 cagtacttga gatctcaacc aaaatcaaat catcatcact aaactcattg tcattacgcc
    51241 agataatgag tgatccaagg cgcatccctc caccataaat cggcgcaata gtcgttagac
    51301 ctcctggata agtgtcttta gactcaacag ggaatatagt caattcattt tcgacagaaa
    51361 ggttagcttc tgtatcgtaa actcggctag cagcctttac ataagtatct ggaaattgtt
    51421 tagcttcaaa aaattcctca acacgatcag tgttagtttt atatttcatg gcataaccaa
    51481 gcagtgtgcc gccgccattg ataatacaag cattgcagtc aatgatatct gctaggcgag
    51541 atgccattgt gttatacggt aattctgttt ctaggctatc tacggaacgc tgcaaaatag
    51601 atgtgatttt acgagttttt tctaataagt taggcatttt tttccttttc ctttgaatta
    51661 tttgcgcttt cattataaca aagctaactc gcttttgcaa ggaattatca gaaaattttc
    51721 tattcttact acaattccaa ctattgatgc cttattatca cttcatttaa aggagataag
    51781 cactagcttg ttagagcata gggtcacatt cttttaagaa acgttatttt tattaatcaa
    51841 agatgagcat tgcattaacg tctatactgg tataagacac gtgtaatctt ttcttggaca
    51901 tcttctaatt cttctacact tggtaggtaa acaatacgga agtgatctgg atctttccag
    51961 ttaaaaccac gtccatgtac caacatcact ttttcttgct ttaataactg taagacaaat
    52021 tcttcatcat catcaatacg atacatctga cgatcaattt tcggaaataa ataaagacct
    52081 gcctcaggtt taactgccga taagccaggg atagcattaa tagcttcata aataaaatta
    52141 cgttgctcaa aaatacgccc tcctggcagt aacagtgcat caactgattg atgacctcct
    52201 aaggaagttt gaacaacttg ttgggctaaa acattggagc ataaccgcat attggcaagc
    52261 atgtttaacc cttcaatgta acctttaaca tggtctttag gacctgataa gaccatccat
    52321 ccaacacgaa aaccagcaat acgatgagat ttggaaaggc cattcataga tacacaaaaa
    52381 acatcaggtg ccaaacttgc aatagcaata tgctctttac tatccatcac caaacgatca
    52441 tagatttcat cggcaaaaat aatcagctgg tgttctcttg ccaaagcaac gatgtcttct
    52501 aaaatctcct taggataaag agctcctgtt gggttattag gattgatgac cacaatagct
    52561 ttggtgcgac tcgtgatttt tgatttgata tcagcaatat caggatacca accagcttct
    52621 tcatcacaaa gataatggac cgcttttcct ccaccaagac tcacacaagc cgtccataaa
    52681 ggataatccg gcattggtac taaaacttca tcgccattat ctagcaaagc ttgaagggac
    52741 atggatatca actctgacac gccatttccc aaataaatat cttcaatgtc aacatctggg
    52801 aagcccttga gttgacaata ttgcatgata gccttacgcg ctgaaaaaat ccctttactg
    52861 tcagaatagc cctcacttag cctcgcgttg acaattaaat cacgaatcac ttcatctggt
    52921 gcttcaaaac caaaggcagc aggattgcca gtatttaaac gcagaatttt ttcaccactc
    52981 gcaatcatgc gattagcttc atccaaaaca ggccctcgaa tatcataggc aacatgttct
    53041 aattttgatg atttttcaat aattttcata gtctcataca cctcgtattc catattataa
    53101 accaaaatac ttaaaataga aagaatttat ccatcaaagc ttaggacagc taatcgtttt
    53161 aacacaggtc ttatgagacg ataatacagt ctttagttta gcaaaaataa tggatcgctt
    53221 tcaaaatgac tcaaaaaaga gtataatgaa aaaaagaata aaagctattc ttgtcaaact
    53281 gttaaaggag gacagttcta tgtcactaaa atataaacgt atcctcgttg ctattgatgg
    53341 ctcttatgag tctgaattag ctttcaataa aggcgttaac gtagcactac gtaacgacgc
    53401 taccttacta ctagtacacg ttattgacac ccgtgccttg caaagcgtag ctacttttga
    53461 tacttacatc tatgaaaaat tagaacaaga agctaaggac gttttagatg attttgaaaa
    53521 acaagcacag atagcaggaa ttacaaacac caaacaaatt attgaatttg gcaatcccaa
    53581 aaacttacta gctcacgaca ttccagatcg tgagaacgct gatttaatta tggtaggagc
    53641 tactggtctt aatacctttg aacgcctcct tattggctca tcttctgagt acattatgcg
    53701 acacgctaag attgacttac ttgtagtccg agatagcact aaaacactct agtctattac
    53761 aattttcaaa caccaaaaaa caagcagcat ctacaacgaa attgcttgtt ttttggtgct
    53821 gagctaagtc tagtcaatat ctttttattt cttgatattt aaaagagtaa acctctattt
    53881 atcgaatcgt tgtagtgatt tttgtaattg tttgtctaac tcttttttta tggctacttc
    53941 aggtgcgtga cgctcttgcc aatagtcagg tttttgaatt ttatgggtaa cggggtcaaa
    54001 gtgtgccttg ccatctggaa aaattttagc catatttgct tcatgtactg cttcaaatat
    54061 aggttgtgga tctactccca tcagtacaaa agaaccataa gtaaaataaa gcaaatccgc
    54121 taaggcatca acttgcccaa tcaggggcgt ttcaacatgt tttttagctt gtaccttatt
    54181 aactgcttgg tcaacagcac catgcaaatc gaaaatagcc tgtgtaaatt gttgttggtc
    54241 tcccttgctt gctgcgtaaa gaaactcaac aatttcttct actttgaagc ctgacctata
    54301 accagcttct gaaatggtat aactacgcgg tgtttcaata gtatctgagt ccatcagacg
    54361 atgaaaatct ttgactttat taaaattttc atcacggctt ttaaagctct tctctacatc
    54421 aaaatggatg agaccgtagt gagctaaggc tttggaaatc ccatcattat tattagagtc
    54481 ggtagtgaaa tgggctccat cttttaccac agtttcagca tttcccatag caataccgat
    54541 accaactcct gataacattt caaggtcatt atctgaatct ccaaaagcca tcacttcaga
    54601 caagtcaaat ccaaacatct ctcccaaacg ttcaattcct tttatttttg attgatcaac
    54661 agagatcaga tccaaggaat aaggactact tcttgtaatt tttatatgag ggaacttctc
    54721 ttgtattttc ttagtttcag cttgtgacgc taccagaaca acctgataaa taggctcccg
    54781 cataatagtt actaaatttc taaaactctg aggcttgatt cggcgaatca aatgcttaaa
    54841 actaccctca actgttcttg cccaactttt gggcacaaaa ctactaatga cttgtccaaa
    54901 tgggctagtc cccatatcaa taatacgcga tcctgctaat ccagaagctg ttccaagaga
    54961 aatttctcgc tttttttcgt tggcatagcg aatcactttg taaatcatcg actttggcaa
    55021 ttgattctga tataagacct tatctcttgt taagatatat tgcccattgt aagtaacggc
    55081 aaaatctaat ccaaaatttt ctagaaaagg ttgcacaaat cccggtccac gaccagttgc
    55141 taaaccgacc ataatgcctt gttttttgag ttgttggatg gctttttgcg ttgttttttg
    55201 gatattttta cgatcattga gaagggtccc atcaatatca aaaaagactg cttttactgt
    55261 caaaaccttc ttttgcctcc tgtgtttatg atagaataga ttatatcata aataaaagga
    55321 agtttgacaa tcaaatgaaa aaaatcctag tcttacacac tggtggaacc atttctatgc
    55381 aagctgataa ctctggtagg gttgttccaa atcaagataa tcctatgacg aaaatacatg
    55441 ctgcagccca agatattcag cttaccgtta gtgattttct aaatcttccg agtcctcaca
    55501 tcacgcctca ccacatgtta tctatttatc atcatattca agaaagaact gatgtctttg
    55561 atggtatagt catcacacat ggaacagata ccttagaaga aacagcttat tttttagata
    55621 ccatggcatt gcctactaat atacctgttg tcctaacagg tgctatgagg tcttctaatg
    55681 aagttggtag tgatggcata tataattacc ttaccgcact tagagttgca agtagtgata
    55741 aagctaaaga aaaaggggta cttgttgtta tgaacgatga aatccatgct gctaaatatg
    55801 ttactaaaac acatacaacc aatatttcaa ctttccaaac acccacacac ggaccacttg
    55861 gtatcatcat gaaaaatgac ctactttttt tcaaaactgc tgaacctcgt attcgttttg
    55921 acttgagatg catttctgga accatcccca ttatcaaagc atacgcaggt atgggcgatg
    55981 gtagtatctt gagtctatta actccaggta gcattcaagg tctagttatc gaagctctag
    56041 gagcaggaaa tgttccacca ttagcagttg gggagatcga gcacttaatt actttaggta
    56101 ttcctgtcgt tcttgtttcc cgttgtttca atggtatggc agaacctgtc tatgcttatg
    56161 aaggtggtgg tgcgatgcta caagaagcag gtgttatgtt tgtaaaagaa ttgaatgctc
    56221 caaaagcccg tctaaagtta ttaattgctc tcaacgccgg acttactggt caagagctaa
    56281 aagattatat tgaaggataa tctagcttcc tgtagaaatg tcccactaaa acatattagt
    56341 gggacatttc tagtacttta agctttaaat taggacatct cctaaatgtc tataataaac
    56401 aactatgttt caaactatga gattgtgcac tcataatcat ttctataaca gtttgagacc
    56461 agtattccaa taaatccaac tcttagcaat cagataccgg gtaaacaagt ctttattttg
    56521 tctcattaag tataagctta cttgaataat accaaaacta cttgctcatc cttttttagt
    56581 aatgatgaag acgacctttt tctaagaact tctttgtctt ttaaaataaa caaagcacat
    56641 attacccttt attttagttc tcgtttggaa aaaagaaaga cattgcctaa aagataacaa
    56701 agagttaaac caaaggccac taatatgcta gtactagaaa gatgtttatc accaagagac
    56761 aaggataggt gtttaaacat caaaactggg ctaatatcta acttaaaaaa atggtttgct
    56821 aaagtagcag atactttatc aaaaagaagg acataaagta tataagctgt gattgactga
    56881 taagttttac gaagtatatt aaacaaaaat tgacacaaag caataatagc aatataaaaa
    56941 ggcagttgtt taatcatccg atctatgact attccatagt atgcttgacc agtaaataaa
    57001 tgaaatgcta aagcactaag aagataagtt aaactaataa ataaaagaga taagccaaaa
    57061 ctaactagca gtttatagat tataattaaa gtacgatttt gttgtttgac cacaacatta
    57121 ttaatcgtac ggtagttata gtcatcacta aaaaagatac gggctggtgt taaaaagaaa
    57181 agcgtcacaa aatcagtaat aatattaatg atttgtatca tgatgacttg gttaggtgtc
    57241 ttgcgaaaac tataggctac accaacagca atgacaacaa gtagcataca agaaaataga
    57301 gctaaacgct cttggcgtat ccgaaataaa tctgctttaa gataggaaat cataaaaacc
    57361 tctttctaaa ttaatggtga cgctgcatct ttacctaaca aatcgaggta ataatgttca
    57421 aagctcactt gatgatgata aatgtcaaaa atctcaacag gcagatctag aacaattttg
    57481 ataagctcca ttactgataa agttggtttt attagtagca tctgaccatc agtctcaact
    57541 ctaagcccca attctaaaag tctatttttt accagctgat catcgcttgt atgaagggca
    57601 atttgtactt ttacttgagc tttaagctct gctttgtcca tactcttaat aactttgcct
    57661 ttatgcataa tcacgtaacg atcaacaact aagtctaatt ctgataaaat atgactggaa
    57721 atcaaaatcg taataccata gcaatctctt aggttcaaaa taatctgacg catttctttg
    57781 attcctgctg gatcaagacc attaataggt tcatctaaga ttaaaaattc tggaaaatcc
    57841 agtatcgaaa gaccaatagc caaacgttgg cgcatcccca aagaataatc tttcaccttt
    57901 ttctttttgc tcactttttc tagcccaata agttccaaaa cctcatatat gcgctctttt
    57961 gcatcagcta atctcaattg caacgctgca tagtaaagat tatcatgagc tgtcatattg
    58021 ggataaagtg tcggtgactc aataattgat cccattcgat agggttgtaa ctgtttgatt
    58081 tgccctgaat cttttccaat taggccagat aaaatacgca ttaacgttgt ctttccagca
    58141 ccattttgac ccacaaggcc gcaaatttct cctttgtaaa tatcaaaaga gatatcactt
    58201 aaaacctgat ttttaccata ggatttattt acttgctcaa tacttagtaa cattgattgc
    58261 tgtgtcattt tccgctcctt ttttgtactt atcgattaat a
//
LOCUS       DAUGHY010000004        54347 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.004, whole genome shotgun sequence.
ACCESSION   DAUGHY010000004 DAUGHY010000000
VERSION     DAUGHY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54347
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.004"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            75..710
                     /locus_tag="VH752_000231"
     CDS             75..710
                     /locus_tag="VH752_000231"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEQ8288614.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQEILTTREPGG
                     VAISEYIRELILDINHTTMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDS
                     SVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            728..1603
                     /locus_tag="VH752_000232"
     CDS             728..1603
                     /locus_tag="VH752_000232"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEQ8288615.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAK
                     VIFCEQKKDQKPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     NKRQVFIIKDCDKMHINAANSLLKYIEEPQGEAYIFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWL
                     KDQLQAYLALNRLVQLATEKEEQDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            1622..1862
                     /locus_tag="VH752_000233"
                     /pseudo
     CDS             1622..1862
                     /locus_tag="VH752_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tpl protein"
     gene            2267..2590
                     /gene="yabA"
                     /locus_tag="VH752_000234"
     CDS             2267..2590
                     /gene="yabA"
                     /locus_tag="VH752_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEQ8288616.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            2595..3458
                     /gene="rsmI"
                     /locus_tag="VH752_000235"
     CDS             2595..3458
                     /gene="rsmI"
                     /locus_tag="VH752_000235"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEQ8288617.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSILEILSYL
                     EETPLKGECLLIVAGAQVDSEVELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVN
                     RQELYQQFHDL"
     gene            3485..3877
                     /locus_tag="VH752_000236"
     CDS             3485..3877
                     /locus_tag="VH752_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288618.1"
                     /translation="MTEDQATILILGVYGIILAVFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFVKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIASLIVAFGKTYTTQECHPVPHLLN"
     gene            complement(3924..4553)
                     /locus_tag="VH752_000237"
     CDS             complement(3924..4553)
                     /locus_tag="VH752_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEQ8288619.1"
                     /translation="MIKEFCAENLTLLPTLDAGQVSRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKKISVATMIRPRGGDFVYNDLELKAMEEDILKAVEAGSDALVLGLLTTENH
                     LDTDAIEQLLPATQGLPLVFHMAFDHIPTDHQHQALDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKSLVTYANKRIEIMIGGGVTAENCQNLSQLTGTAIVHGTKII"
     gene            4852..5208
                     /locus_tag="VH752_000238"
     CDS             4852..5208
                     /locus_tag="VH752_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEQ8288620.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEVIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGR
                     VLQVGYRKPYQELDLG"
     gene            complement(5282..6109)
                     /gene="xth"
                     /locus_tag="VH752_000239"
     CDS             complement(5282..6109)
                     /gene="xth"
                     /locus_tag="VH752_000239"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEQ8288621.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQE
                     TKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEI
                     GAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQK
                     PVLATGDYNVAHKEIDLANPSSNRRSPGFTDEERQGFTNLLARGFTDTFRHLHGDIPH
                     VYTWWAQRSKTSKINNTGWRIDYWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL"
     gene            6343..7524
                     /gene="lctO"
                     /locus_tag="VH752_000240"
     CDS             6343..7524
                     /gene="lctO"
                     /locus_tag="VH752_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEQ8288622.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDHLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVDQKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSTGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            7789..12732
                     /gene="scpC"
                     /locus_tag="VH752_000241"
     CDS             7789..12732
                     /gene="scpC"
                     /locus_tag="VH752_000241"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HEQ8288623.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLIGSVFLMMTTTVAADELTTTSEPT
                     ITNHTQQQAQHLTNTELSSAESKPQDTSQITLKTNREKEQPQGLVSEPTTTELADTDA
                     APMANTGPDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAHQS
                     MRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFEDFD
                     EDWENFEFDAEAEPKAIKKHKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDDTKY
                     ESHGMHVTGIVAGNSKEAAATGERFLGIAPEAQVMFMRVFANDVMGSAESLFIKAIED
                     AVALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDP
                     LATNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTVKELENRADLNHGKAIYSESVD
                     FKNIKDSLGYDKSHQFAYVKESTDAGYNAQNVKGKIALIERDPNKTYDEMIALAKKHG
                     ALGVLIFNNKPGQSNRSMRLTANGMGIPSAFISHEFGKAMSQLNGNGTGSLEFDSVVS
                     KAPSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIA
                     GASLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNI
                     DGAVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKAKTLRYDTELLTDHVDPQ
                     KGRFTLTSRSLKTYQGGEVTVPANGKVTVRVTMDVSQFTKELTKQMPNGYYLEGFVRF
                     RDSQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGK
                     HFTGLVTLGSETNVSTKTISDNGLHTLGTFKNVDGKFILEKNAQGNPVLAISPNGDNN
                     QDFAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTL
                     LGTAFSGKSLTGAELPDGYYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLSQATFDP
                     ETNRFKPEPLKDRGLAGVRKDSVFYLERKDNKPYTVTINDSYKYVSVEDNKTFVERQA
                     DGSFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKE
                     TLKDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKG
                     LKNNVYNDLTVNVYAKDDHQKQTPIWSSQAGASASAIESTAWYGITARGSKVMPGDYQ
                     YVVTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVRE
                     EVFYLAKKNGRKFDVTEGKDGITVSDNKVYIPKNPDGSYTISKRDGVTLSDYYYLVED
                     RAGNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYY
                     NNSGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKMTMLATSQ
                     ITAHFDHLLPEGSRVSLKTAQGQLIPLEQSLYVPKAYGKTVQEGTYEVVVSLPKGYRI
                     EGNTKVNTLPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTASATPTKTTTSAT
                     AKALPSAGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
     gene            13504..14211
                     /locus_tag="VH752_000242"
     CDS             13504..14211
                     /locus_tag="VH752_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEQ8288624.1"
                     /translation="MSKKKRKHYQVYEGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWAIKKGLHWYLLSSFIL
                     TGIAFGTALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALMPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
     gene            14451..16451
                     /gene="metG"
                     /locus_tag="VH752_000243"
     CDS             14451..16451
                     /gene="metG"
                     /locus_tag="VH752_000243"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEQ8288625.1"
                     /translation="MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
                     EVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDDRMNEMVKNFIEPGLEDLAVSRT
                     TFTWGVPVPSDPKHVVYVWIDALLNYATALGYGQANHANFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDSLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGT
                     AFDADLSQLIDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGD
                     KAQLASVMAHLAASLRVVAHVIQPFMMETSAAIMAQLGLAPVSDLSTLALADFPANTK
                     VVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDA
                     VEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVA
                     NLKPRKMMKKYISQGMILSAEHGDQLTVLTVDSSVPNGSIIG"
     gene            16944..17957
                     /gene="nrdF"
                     /locus_tag="VH752_000244"
     CDS             16944..17957
                     /gene="nrdF"
                     /locus_tag="VH752_000244"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ8288626.1"
                     /translation="MTQHYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRARVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYESPFTDEETRVSPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            17961..18449
                     /gene="nrdI"
                     /locus_tag="VH752_000245"
     CDS             17961..18449
                     /gene="nrdI"
                     /locus_tag="VH752_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ8288627.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVPKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFALAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQKQTNNLITERTHPCHKPMRHT
                     SH"
     gene            18416..20596
                     /gene="nrdE"
                     /locus_tag="VH752_000246"
     CDS             18416..20596
                     /gene="nrdE"
                     /locus_tag="VH752_000246"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ8288628.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRVYGKDMSDISITEEYDKLLANPAIKK
                     TYISARKLFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAP
                     SVETGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYHAFKASCQLA
                     KEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTECHSCMI"
     gene            complement(20801..21553)
                     /locus_tag="VH752_000247"
     CDS             complement(20801..21553)
                     /locus_tag="VH752_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEQ8288629.1"
                     /translation="MLKKRYQLAMILLLSCFSLVWQTEGLVELFVCEHYERAVCEGTP
                     AYFTFSDQKGAETLIKKRWGKGLLYPRAEQEAMAAYTCQQAALINTSLDKTKGELSQL
                     TPELRDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHADLTSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTALKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDHKKLHIEAYFKGSL"
     gene            22013..22447
                     /locus_tag="VH752_000248"
     CDS             22013..22447
                     /locus_tag="VH752_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288630.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLMGRPVNAEVTYTGERSTSVN
                     GPGEDSSSEKTEQTSDSDKRDRNRGYEEGYKRGQESDNRVLDRGTIEVPYNVIDGVEY
                     KDGYEQGFAAGWYDGHPITALLDIAWSYLTYAFDVLFGGTNS"
     gene            22890..23303
                     /locus_tag="VH752_000249"
     CDS             22890..23303
                     /locus_tag="VH752_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288631.1"
                     /translation="MTKAIKLVILSLILFTTVFLSVRQASADLMMDGVTYSESTDSNT
                     FLSNSGEVDNIWEKDRDFGKQRGLEAGKNGLGPTINRGDIPVPEKVKHKDKYQDGYQE
                     GYIQAWHQEHPFQGVIFAVFDVFSLIWGVISELFT"
     gene            23544..23795
                     /locus_tag="VH752_000250"
     CDS             23544..23795
                     /locus_tag="VH752_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288632.1"
                     /translation="MPEKKVLPVLSMVVSLVSLFDHIFFFATYSYLYLPLVASSLALI
                     GLVQNKGRQKVWSLAGLILAALAVAVVAYAYYDPTVIPY"
     gene            complement(23889..24521)
                     /locus_tag="VH752_000251"
     CDS             complement(23889..24521)
                     /locus_tag="VH752_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288633.1"
                     /translation="MKKTLTLLLALFAIGVTSSVRAEDEQSSKQKPVKFDLDEPQQKI
                     KDYSGNTITLEDLYVGSKVVKIYIPQGWWVYLYRQCDHNSKERGILASPILEKNMTKT
                     DPYRQYYTGVPYILNLGEDPLKKGEKLTFSFKGEDGFYVGSYIYRDSDTIKKEKEAEE
                     ALQKKEEEKQQKQLEESMLKQIREEDHKPWHQRLSESIQDQWWNFKGLFQ"
     gene            complement(25033..26001)
                     /locus_tag="VH752_000252"
     CDS             complement(25033..26001)
                     /locus_tag="VH752_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HEQ8288634.1"
                     /translation="MENLSERLVLKSVEAFIMGLEIYNKPTIKYRIEGFSFFICNAWE
                     LMLKAEMLNRGFSIYYSDNPERTLSLEYVLKKIYSDENTRIRLNLEKIIELRNISTHF
                     ITEDYEIKYAPLFQACVLNLASEMKRFHNKDITESIAHNFLTISASYEPLSNEEIKLK
                     YPSEIAEKFIKQANEVDVLSKEYNSEKFSIDIRQNLYITKKKDEADFVVSISKDAETP
                     VSLVKELKDPSDTHKYSYSNVVTAVQERLKKSAIKLGYEKGFNTYVLNLVIDFYNIKS
                     ETKFSYCHLIGSTQTYTYSQQFVEFIVNEIKKDPQNFVESLKKSNK"
     gene            26613..26849
                     /locus_tag="VH752_000253"
     CDS             26613..26849
                     /locus_tag="VH752_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEQ8288635.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGAGEGFSMWAPTPCDVLAEDWIEVND"
     gene            26842..27540
                     /locus_tag="VH752_000254"
     CDS             26842..27540
                     /locus_tag="VH752_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEQ8288636.1"
                     /translation="MTKVVLVTGCASGIGCAQARYFLRQGYHVYGVDKSDKPDLNGNF
                     HFIKLDLSSELSPLFKVVPSVDILCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRRMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTASDFEPGGLADWVARETPIGRWTEPDEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            27616..28575
                     /locus_tag="VH752_000255"
     CDS             27616..28575
                     /locus_tag="VH752_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ8288637.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLKTAKDLA
                     KAYHMPLATSKYEAVLGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVVIAKKRKLILLEAITNQYLSNMTFIKEHLDQLGDIKIVECNYSQYSSRYDAF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANVEKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKSTIQGNKGSLAVLGATNTLPQVQLSLHGQKPQVINHNKH
                     DHRMYEEFVAFKDMIDQKDFKKVNQALEHSRAVMAVLERAVHS"
     gene            28908..30245
                     /locus_tag="VH752_000256"
     CDS             28908..30245
                     /locus_tag="VH752_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ8288638.1"
                     /translation="MEEDYNKREPEKFTQFLRRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIATLLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGVLVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISILEWVAIPGSLVFAWLAVRYPNKMAKIGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            30419..31801
                     /gene="glmU"
                     /locus_tag="VH752_000257"
     CDS             30419..31801
                     /gene="glmU"
                     /locus_tag="VH752_000257"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEQ8288639.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIS
                     PEKAVTVIGHKSEKVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILR
                     DFNESLGVNDRVALATAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGQGSIITNSMIESSVLAAGVTVGPYAHLRPG
                     TTLGREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDNALTAAGSTISKTVPADSIVIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            31835..32389
                     /locus_tag="VH752_000258"
     CDS             31835..32389
                     /locus_tag="VH752_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ8288640.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKIVLVKQYRKAIESVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLTQVANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            32389..32640
                     /locus_tag="VH752_000259"
     CDS             32389..32640
                     /locus_tag="VH752_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288641.1"
                     /translation="MGRPLLTDDIIEKARRMETFEPDDAVDFDTKVMTLPEKDEKARI
                     YKSRRIENAKRSQLQSKLNVILIAVMLLIAILVYAIFYL"
     gene            32660..33355
                     /locus_tag="VH752_000260"
     CDS             32660..33355
                     /locus_tag="VH752_000260"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEQ8288642.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELI
                     LVQSGVGKVMSAMTVAILAEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDA
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQILMTFLENLPV"
     gene            33468..33686
                     /locus_tag="VH752_000261"
     CDS             33468..33686
                     /locus_tag="VH752_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288643.1"
                     /translation="MTGPKSHLQYSKDNTKLQLRNILNGYQSDLGRHYSSYYYYNLRT
                     VMGLSSEQEIEEHYEKLKNKLHDMYNHY"
     gene            complement(33783..34430)
                     /locus_tag="VH752_000262"
     CDS             complement(33783..34430)
                     /locus_tag="VH752_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEQ8288644.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDTFIDRLDKILDFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLEAHHLSINTELTLTQIDTFAKTYTICYGDKELVIPENIAKQLYVTAL"
     gene            34576..35508
                     /locus_tag="VH752_000263"
     CDS             34576..35508
                     /locus_tag="VH752_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEQ8288645.1"
                     /translation="MGKRMSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIA
                     DMTKAIAGDKIDLHSIVPIGQDPHEYGPLPEDVEKTSNADVIFYNGINLEDGGQAWFT
                     KLVKNAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLI
                     AKDPKNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGV
                     PSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYS
                     EIFTDSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            35572..36297
                     /locus_tag="VH752_000264"
     CDS             35572..36297
                     /locus_tag="VH752_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ8288646.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIEGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            36298..37152
                     /locus_tag="VH752_000265"
     CDS             36298..37152
                     /locus_tag="VH752_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ8288647.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLVVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(37300..38106)
                     /locus_tag="VH752_000266"
     CDS             complement(37300..38106)
                     /locus_tag="VH752_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEQ8288648.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPEQDITITSIDIVKDYRFKN"
     gene            38323..40728
                     /locus_tag="VH752_000267"
     CDS             38323..40728
                     /locus_tag="VH752_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ8288649.1"
                     /translation="MVKRNQRRKSAPKKRLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMFDQDIFLSTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHFVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMTKEPEILAYDSHLK
                     DDEASLFDQEDLAYAHEKIGAYDSLSALASSEDEMDMDEPVEVDFTPKTHLLYKLPTI
                     DLFAPDKPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSVTKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FLMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKVGVRNIA
                     GYNAKVEDYNRQSEQKQMPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGAAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(40798..41151)
                     /locus_tag="VH752_000268"
     CDS             complement(40798..41151)
                     /locus_tag="VH752_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEQ8288650.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTPLFKLNKKGFKFPDVALLLFA
                     IEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
     gene            41395..41820
                     /gene="rplK"
                     /locus_tag="VH752_000269"
     CDS             41395..41820
                     /gene="rplK"
                     /locus_tag="VH752_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEQ8288651.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            41926..42615
                     /gene="rplA"
                     /locus_tag="VH752_000270"
     CDS             41926..42615
                     /gene="rplA"
                     /locus_tag="VH752_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEQ8288652.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            42937..43665
                     /locus_tag="VH752_000271"
     CDS             42937..43665
                     /locus_tag="VH752_000271"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEQ8288653.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            43694..44251
                     /gene="frr"
                     /locus_tag="VH752_000272"
     CDS             43694..44251
                     /gene="frr"
                     /locus_tag="VH752_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEQ8288654.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKATDDAIKEIDRMTAEKEKELLSV"
     gene            44360..45217
                     /locus_tag="VH752_000273"
     CDS             44360..45217
                     /locus_tag="VH752_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ8288655.1"
                     /translation="MNDLLATVITGLIKEENANDYFIQKEGFTFTLSKAEGERQIGDM
                     VTGFAYTDIKQKARLTTKEIRSTRTSYGWGEVTEVRRDLGVFVDTGIPNKEIVVSLDV
                     LPEMKELWPKKGDKLYISLDVDKKDRIWGLPAEPEVFQKMASPAYNNMQNQHWPAIVY
                     RLKLTGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKRIKQ
                     DATGTELIG"
     gene            45290..45799
                     /gene="msrA"
                     /locus_tag="VH752_000274"
     CDS             45290..45799
                     /gene="msrA"
                     /locus_tag="VH752_000274"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEQ8288656.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPKQIAYKDLVELYWAQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPKRYAQSSAIR
                     HQFLEENWS"
     gene            45796..46011
                     /locus_tag="VH752_000275"
     CDS             45796..46011
                     /locus_tag="VH752_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEQ8288657.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            46168..47337
                     /locus_tag="VH752_000276"
     CDS             46168..47337
                     /locus_tag="VH752_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ8288658.1"
                     /translation="MIITKKSLFVTSVALSLAPLATAQAQEWTPRSVTEIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATTL
                     TVQAPASSPASVSHVPSSEPLPQASATSQSTVPMAPSATPSDVPTTPLASAKPDSFVT
                     ASSELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEDPTSANRP
                     VPNESASEEVSSAAPAQAPAEKEETSAPAAQKAVADTTSVATSNGLSYAPNHAYNPMN
                     AGLQPQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPENSALGDQVAQYA
                     IDHMAERGISYVIWKQRFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFNA"
     gene            47652..49424
                     /locus_tag="VH752_000277"
     CDS             47652..49424
                     /locus_tag="VH752_000277"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEQ8288659.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIHLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELIHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWVYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEAKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKENETTVWIYGLYSNSKGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLI"
     gene            49583..50635
                     /locus_tag="VH752_000278"
     CDS             49583..50635
                     /locus_tag="VH752_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEQ8288660.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEETKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            50681..51256
                     /locus_tag="VH752_000279"
     CDS             50681..51256
                     /locus_tag="VH752_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEQ8288661.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIV
                     AQETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEEYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            51415..51912
                     /gene="ybeY"
                     /locus_tag="VH752_000280"
     CDS             51415..51912
                     /gene="ybeY"
                     /locus_tag="VH752_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEQ8288662.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            51893..52300
                     /locus_tag="VH752_000281"
     CDS             51893..52300
                     /locus_tag="VH752_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEQ8288663.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            52420..53316
                     /gene="era"
                     /locus_tag="VH752_000282"
     CDS             52420..53316
                     /gene="era"
                     /locus_tag="VH752_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEQ8288664.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            53336..53812
                     /locus_tag="VH752_000283"
     CDS             53336..53812
                     /locus_tag="VH752_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ8288665.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKT
                     WAIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     gene            complement(54118..54297)
                     /locus_tag="VH752_000284"
     CDS             complement(54118..54297)
                     /locus_tag="VH752_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288666.1"
                     /translation="MRYPHAHIILIGNHINYEEIFKNHYRVFGVIDTTSHKSSKSIRD
                     QIQLYLDELYNSNNG"
BASE COUNT        16711 a        10288 c        11599 g        15749 t
ORIGIN      
        1 actgcaaaga tatgccagtg ctttttagct cattttttga taaaatagaa ctactaaaaa
       61 gtgaaggaaa taagatgatc acaggaaaac tcataaccgt tgaaggtcca gatggcgcag
      121 ggaaaacgac tgttttagag caactcattc cactgttaaa acaaaaagtg gctcaggaga
      181 tcttgacaac aagagagcct ggaggtgttg ccatttcaga atacattaga gaacttattt
      241 tagacatcaa ccatactact atggacccta aaacagaact cttactttat attgcggctc
      301 gaaggcagca tttggtggaa aaggtactac cagctcttga agcaggccag ttggttttta
      361 ttgatcgttt catcgatagt tcagtagctt atcaaggtgc aggacgcggt ttgatcaaag
      421 cagacattca gtggctcaac gagttcgcaa cagatggttt agagcctgac ttgacccttt
      481 attttgatgt tccttctgaa attggtttag cacggattaa cgcgaaccaa caaagagaag
      541 tcaatcgttt agatttggaa acaatagaga tacatcaacg agtacgaaaa ggatacctag
      601 ccttagctaa ggagcatcca aaacgtattg tcacaattga tgcgactaaa ccattgaaag
      661 aagttgtctc tgtcgctttg gaacatgtct tagccttgct tttagcctag gataagggag
      721 ggtaggaatg gatttagcgc aaaaagctcc taacgtttat caagcttttc agacgatttt
      781 aaagaaagac cgtctgaatc atgcttatct tttttcgggt gattttgcta atgaagaaat
      841 ggctcttttt ttagctaagg tcatcttttg tgaacagaaa aaggatcaga agccctgcgg
      901 gcattgtcgc tcttgtcaat tgattgaaca aggagatttt gccgatgtga cggtattgga
      961 accaacaggg caagtgatta aaacggatgt ggtcaaagaa atgatggcta acttttctca
     1021 gacaggatat gaaaacaaac gacaagtttt tattatcaaa gattgtgaca aaatgcatat
     1081 caatgccgct aatagcttgc taaaatacat tgaggagcct cagggagaag cttacatatt
     1141 tttattgacc aatgatgata acaaagtgct tccgaccatt aaaagtcgga cacaggtttt
     1201 tcagtttcct aaaaacgaag cctatcttta ccaattggca caagaaaagg gattattaaa
     1261 ccatcaggct aagttagtag ccaaacttgc cacaaatacc agtcatctag aacgtctgtt
     1321 gcaaacgagc aagcttttag aactgataac tcaagcagag cgttttgtat ctatttggct
     1381 gaaagatcag ttgcaggcat atttagcgtt gaaccgtctg gtacagttag caactgaaaa
     1441 agaagaacaa gatttagttt tgaccctttt gaccttgctc ttggcaagag agcgtgcgca
     1501 aacgcctttg acacaattgg aggctgtcta tcaggctagg ctcatgtggc agagcaatgt
     1561 taattttcaa aacacattag aatatatggt gatgtcagaa taagtgcaat tggaattcgt
     1621 tatggggaaa cgaagcttgt caaaagactt gcccgtagcg accaaagtag cggcgcatag
     1681 gggcaaaaca agcttttgaa gaaaatactg cctttgcaac agatttgatg aaagaagtat
     1741 cacgattaat aacacaaaat caacttaaaa tgacgtttat taatagtgtc tttcccttag
     1801 atcagcatgt tgttctaatt cccttttcag cagaagagca catggatttt tacaactgtt
     1861 aaaagattta gcggccgatt ttaggtcaag aagtgagctg ccccagatta acagccaaga
     1921 agatttcatt ttctatggag gctatggacc gtgtggcagg ccactgtgct gctttcgttt
     1981 ttgggaaaat ttctgacagt atctattaaa atactaaaaa atcaagggtt atcattgact
     2041 ttgggtaaag caacaagccc ttatggccgt ttgctttgct gtctctatga tgaaggtggc
     2101 ttttaccaat aaagcaagaa aaaattccct gaatatggta ccatagtaga gacaagtgat
     2161 ggccctgcaa cagtagcagc cgttgatata tttacagatc tcattaaggt gcggttaaaa
     2221 gaccagttga ccttagtcac ttatgcttta gaggaggtta aagtaggtga ataaaaaaga
     2281 attattcgat gcttttgatg gattttccca aaatttaatg gttactttag ctgaaattga
     2341 agccatgaaa aaacaagtcc aatcactagt agaagaaaac acgattcttc gcctagaaaa
     2401 tacaaaacta cgggaacgtt tgagtcacct agaacatgaa acggtagcta aaaatccttc
     2461 caaacaaagg aaggatcact tagagggaat ttatgatgaa gggtttcata tctgcaattt
     2521 tttctatgga caacgtcgtg aaaatgatga agaatgtatg ttttgcagag aattattaga
     2581 tagaaagtaa gggcatgcag gttcaaaaaa gttttaaaga caaaaaaaca agcggaacct
     2641 tgtatttggt tccaacccca attggcaatc tgcaggacat gacctttaga gcagtggcaa
     2701 cgttaaaaga ggttgatttt atttgtgcag aagacacaag aaacacaggt cttttgctaa
     2761 aacactttga cattgctacc aagcaaatca gctttcatga gcacaacgcc tatgagaaga
     2821 ttcctgattt aattgattta ttaatatcag gacggtcact agctcaggta tctgatgcag
     2881 gaatgccatc gatttctgat cctgggcatg atttggtcaa ggctgctatt gatagtgata
     2941 tcgcagtcgt tgctttacca ggcgcctcag cagggattac agctttgatt gctagtggac
     3001 tggctcctca accccatgtt ttttatggat ttttacccag aaaagcaggg caacaaaagg
     3061 ctttctttga ggacaaacac cattacccag agacacaaat gttttatgaa tcgccctatc
     3121 gcatcaaaga taccctgacc aatatgttgg cttgttatgg tgatcgtcaa gtggtacttg
     3181 ttagagaatt aaccaagctt tttgaggagt accaacgggg gtcgatttta gaaatcttgt
     3241 cctaccttga ggaaacaccc ctaaaagggg agtgtctgct gattgtggct ggagcccaag
     3301 tagacagtga ggttgagctg acagctgatg ttgacttagt gtctctagtg caaaaagaaa
     3361 tccaagcagg agccaagcca aatcaggcta ttaaaacaat tgctaaagct taccaggtaa
     3421 atcgccaaga gctttaccaa caatttcatg acttataagt catcgtagat taggagaaaa
     3481 acaaatgaca gaagatcaag caactatcct tattttagga gtatacggaa tcatattggc
     3541 tgtattctta ttatcagcct tattatgcct tatcgctaac tggcgactct ttaaaagagc
     3601 aggttacaaa ggatggtata gtctcattcc tttctatgca ggttttgtca aacacaaaat
     3661 cacttttgga gaagataata aatggtttta ctttattggt tgggtcattg ctatttatta
     3721 ttactatgtg cacttttgct atgtccgtgc ttttggtgga tcaaagggtc tagcagtatt
     3781 tggcttgttt ttcccaggca ttgcttcttt gatagtggct tttggaaaaa cttataccac
     3841 acaagaatgc caccctgtgc cacatctttt aaactaagag aaaaaagacc atcaattgga
     3901 tttattgatg gtcttttagg tgtttaaatg attttagtcc cgtgaacaat ggccgttcca
     3961 gtgagttgac tcaagttttg gcaattctca gcagtgacgc ctcctccaat catgatttca
     4021 atgcgtttat tggcataggt cacaagactt ttaagctgtt ccacattatc tgtaatagga
     4081 gtagcttcag gggagccatg agttagcaca cgcacaaagc cataatcaat cagttgatca
     4141 agcgcttggt gctggtggtc agtcggaata tggtcaaaag ccatgtggaa aaccaatggc
     4201 aagccttgtg tagcaggcag taactgctcg atagcgtcgg tatcaaggtg attttctgtg
     4261 gtcagcaagc ctaagactag cgcatctgag ccagcttcaa cagcttttaa gatatcttct
     4321 tccatagctt ttagttcgag gtcgttatag acaaaatcgc caccgcgagg tcgaatcatg
     4381 gtcgctacgc taatcttttt atcgtgtaag agctgacagg cttctttgat gacgccataa
     4441 gatggtgttg ttcccccgac agctaggtta tcacagagtt ccacacgact gacttgacca
     4501 gcatcaaggg ttggcagtag tgtgaggttc tcagcacaaa attctttaat catttttaga
     4561 tctcctttat cactaatatt atagcacgaa tgaagctagt catcaggaaa catttttgtc
     4621 aaatcatgga cattatgtta tactcactag tattaaaagt aggccaaaac agcagcaaat
     4681 actttgaaaa aagattgcta tgttctacgg caatatcagt ctttaccaac gaactctcaa
     4741 gaagaaaaga aagacaaagc ttagcattta aaaaaagccc cccttttttt gtgaaatgat
     4801 gaccaaaaac aggcgcctca cgcctaaaac agttacccta aaggagaaca aatgattacg
     4861 atttaccaat acccaaaatg tagtacctgt caaagagcca tggctgaact aaagcaactg
     4921 gttagtgact ttgaagtcat tgacatcaaa gctaatccac ccaaagctca ggacctcaaa
     4981 cattggatgg aaacatctgg ttataccatc aaaaatttct ttaataccag tggtaacagc
     5041 taccgcgaat tgggacttaa agataaaata gaccagctta gccttgataa agctgctgaa
     5101 ctcttagcca cagatggtat gctgattaaa cggcctattc tgattaagga cggtagagtc
     5161 ctacaagttg gctatcgcaa gccatatcaa gagttggatt taggataatg cgacaaagtc
     5221 tcaaaaaagg acaaggcaac attataacgc cttgtccttt tttgggatct gtttttagaa
     5281 attagagatc aatatctagt aaaattggcg tatggtcttg gcgttcccca gaagaaatca
     5341 tttcagaacg cttaacctta tccacaagtc ggtttgaagc tagccaatag tcaattctcc
     5401 aaccggtatt attgattttg ctggttttgc tacgttgtgc ccaccaggta taaacatgag
     5461 gaatatcgcc gtgcaagtga cgaaaagtat ctgtaaagcc tcttgctaat aggttagtaa
     5521 agccttgacg ttcttcgtcg gtaaaaccag gggaacgtcg attgctgctt ggatttgcca
     5581 aatcaatctc tttgtgggca acgttgtaat ctcccgtagc caaaacaggc ttttgagcat
     5641 ccagttcagt caagtaatca gcgtatttgt ggtcccaaat ttgacggtca tctaaacgtc
     5701 taaggccatc accagcatta ggcgtgtaaa cctgtgtgac gaaaaagtct tcaaattcta
     5761 gggtaatgat acggccctca gcatccattg ttgttggggc accaatttct ggaaaagtaa
     5821 tgacagggtt tagcgtgttt ttgtacagga acatggtgcc agcatagcct ttcctagcag
     5881 gctcaactga agaacgccac acatgcaaat agtttggaaa gtaactaagt aaggtctcga
     5941 tatgtttttt ggtaggcccc ttagcagata atttggtttc ttgaatggca ataatatccg
     6001 catcttgagc gactaaagtg tctaaaacag cgcgtgataa aagcgcacga ggggactctc
     6061 ctgtgagagc agcatttaag gaatcaatgt tccatgaaat cagtttcatt tggtatctct
     6121 ttctaacaga tagtcttgtt gttattgtaa caaaaaacga aacgataagc gactttggac
     6181 acctagcccc cataaaacag tctagccaaa tgaagcatag cagcgtaaga acaagataag
     6241 gaggtgatca gtgagcatat cgtttgacag ttaagagtag gattactata atagaagcat
     6301 aaatgtaagc gctaacaaag taaaaaatat aggagaatgt caatggcaca aaaaacagtc
     6361 atcactgagg aaacaaccga ttttgttatg gatttcaaaa caagtagcgc tgagggaaat
     6421 gttgatttta ttaacgtttt tgaccttgag aaaatggcac aacaggtcat ccctaaagga
     6481 gcctttggtt atattgctag tggtgctggt gataccttta ccctccatga aaatattcgc
     6541 tcatttaatc ataaattaat tgtccctcac tccctcaaag gggttgaaaa tccgtcaaca
     6601 gagattacgt ttgatggtga tcacttaacc tctcctctta ttttagcacc tgtagcggct
     6661 cataagctgg ccaatgaaca aggagaagta gcgtctgcca aaggattgaa ggaatttgga
     6721 agtatctaca ccactagttc ctactcaaca actgacctgc cagaaatcag tgctgcctta
     6781 ggtgggacac cccattggtt tcaattttat tacagtaaag acgatggcat taatcgtaat
     6841 attatggatc gtgtcaaagc acaaggttgc aaagctatcg ttttgacagc agatgcaaca
     6901 gttggaggaa accgcgaagt tgataggcga aacggctttg tttttccggt cggtatgcca
     6961 attgttcaag aatatttacc tgatggtgca ggtaaaacaa tggattatgt ctataaatca
     7021 gctaaacaag cgcttacctc aaaagatatt gaatatatcg caacttactc aggcctgcct
     7081 gtttatgtca aaggaccaca gtgtgctgag gataccttgc gagcattgga tgcaggagca
     7141 tcaggtatct gggtaaccaa tcatggtggc cgccaattag atggtggtcc agctgctttt
     7201 gattccttac aagaagtcgc tgaagcagtt gatcaaaaag ttcctattgt ctttgactca
     7261 ggtataagac ggggtcaaca tattttcaaa gctcttgctt caggtgctga ccttgttgcc
     7321 ttggggcgtc cagcgattta cggcttggct atgggaggaa gcactggtac tcgtcaggtc
     7381 tttgaaaagt taaatgacga gttaaaaatg gtcatgcaac tggctgggac acagactatc
     7441 caagatgtta aagcctttaa ccttcgccac aatccgtatg attcatcgat tccatttgat
     7501 caaaatgcgc tacgcttaga ttagaacaga caaaaacctc tgctatcatg tcacaaatag
     7561 tagaggtttt acactgcaaa aagaggtatg aaaattcaat aacatagtta caaggctatc
     7621 atttaccacc cccactaata gattaaaatg aaagacttct tacattaata gaagtttctg
     7681 ttgcttttct cagaaaaatg tggttaaata atcaaatatt attaacgaaa aatgagttaa
     7741 tgattaacat taaactggtt ttagttaaaa catttaggag ggtatcaggt ggagaaaaag
     7801 caacgttttt cccttagaaa atacaaatca ggaacgtttt cggtcttaat aggaagcgtt
     7861 ttcttgatga tgacaacaac agtagcagca gatgagctaa ccacaacgag tgaaccaaca
     7921 atcacgaatc acactcaaca acaagcgcaa catctcacca atacagagtt gagctcagct
     7981 gaatcaaaac ctcaagacac atcacaaatc actctcaaga caaatcgtga aaaagagcaa
     8041 ccacaaggtc tagtctctga gccaaccaca actgagctag ctgacacaga tgcagcacca
     8101 atggctaata caggtcctga tgcgactcaa aaaagcgctt ctttaccgcc agtcaataca
     8161 gatgttcacg attgggtaaa aaccaaagga gcttgggaca agggatacaa aggacaaggc
     8221 aaggttgtcg cagttattga cacagggatc gatccggccc atcaaagcat gcgcatcagt
     8281 gatgtatcaa ctgctaaagt aaaatcaaaa gaagacatgc tagcacgcca aaaagccgcc
     8341 ggtattaatt atgggagttg gataaatgat aaagttgttt ttgcacataa ttatgtggaa
     8401 aatagcgata atatcaaaga aaatcaattc gaggattttg atgaggactg ggaaaacttt
     8461 gagtttgatg cagaggcaga gccaaaagcc atcaaaaaac acaagatcta tcgtccccaa
     8521 tcaacccagg caccgaaaga aactgttatc aaaacagaag aaacagatgg ttcacatgat
     8581 attgactgga cacaaacaga cgatgacacc aaatacgagt cacacggtat gcatgtgaca
     8641 ggtattgtag ccggtaatag caaagaagcc gctgctactg gagaacgctt tttaggaatt
     8701 gcaccagagg cccaagtcat gttcatgcgt gtttttgcca acgacgtcat gggatcagct
     8761 gaatcactct ttatcaaagc tatcgaagat gccgtggctt taggagcaga tgtgatcaac
     8821 ctgagtcttg gaaccgctaa tggggcacag cttagtggca gcaagcctct aatggaagca
     8881 attgaaaaag ctaaaaaagc cggtgtatca gttgttgtag cagcaggaaa tgagcgcgtc
     8941 tatggatctg accatgatga tccattggca acaaatccag actatggttt ggtcggttct
     9001 ccctcaacag gtcgaacacc aacatcagtg gcagctataa acagtaagtg ggtgattcaa
     9061 cgtctaatga cggtcaaaga attagaaaac cgtgccgatt taaaccatgg taaagccatc
     9121 tattcagagt ctgtcgactt taaaaacata aaagatagcc taggttatga taaatcgcat
     9181 caatttgctt atgtcaaaga gtcaactgat gcgggttata acgcacaaaa cgttaaaggt
     9241 aaaattgctt taattgaacg tgatcccaat aaaacctatg acgaaatgat tgctttggct
     9301 aagaaacatg gagccctggg agtacttatt tttaataaca agcctggtca atcaaaccgc
     9361 tcaatgcgcc taacagctaa tgggatgggg ataccatctg ctttcatatc gcacgaattt
     9421 ggtaaggcca tgtcccaatt aaatggcaat ggtacaggaa gtttagagtt tgacagtgtg
     9481 gtctcaaaag caccgagtca aaaaggcaat gaaatgaatc atttttcaaa ttggggccta
     9541 acctctgatg gttatttaaa acctgacatt actgcaccag gtggcgatat ctactctacc
     9601 tataacgata accactatgg tagccaaaca ggaacaagta tggcctctcc tcagattgct
     9661 ggcgccagcc ttttggtcaa acaataccta gaaaagactc agccaaactt gccaaaagaa
     9721 aaaattgctg atatcgttaa gaacctattg atgagcaatg ctcaaattca tgttaatcca
     9781 gagacaaaaa cgaccacctc accgcgtcag caaggggcag gattacttaa tattgacgga
     9841 gctgtcacta gcggccttta tgtgacagga aaagacaact atggcagtat atcattaggc
     9901 aacatcacag atacgatgac gtttgatgtg actgttcaca acctaagcaa taaagccaaa
     9961 acattacgtt atgacacaga attgctaaca gatcatgtag acccacaaaa gggccgcttc
    10021 actttgactt ctcgctcctt aaaaacgtac caaggaggag aagttacagt cccagctaat
    10081 ggaaaagtga ctgtaagggt taccatggat gtctcacagt tcacaaaaga gctaacaaaa
    10141 cagatgccaa atggttacta tctagaaggt tttgtccgct ttagagatag tcaagatgac
    10201 caactaaata gagtaaacat tccttttgtt ggttttaaag gacaatttga aaacttagca
    10261 gttgcagaag agtccattta caggttaaaa tctcaaggca aaactggttt ttactttgat
    10321 gaatcaggtc caaaagacga tatctatgtc ggtaaacact ttacaggact tgtcactctt
    10381 ggttcagaga ctaatgtgtc aaccaaaacg atttctgaca atggtctaca cacacttggc
    10441 acctttaaaa atgtagatgg caaatttatc ttagaaaaaa atgcccaagg aaaccctgtc
    10501 ttagccattt ctccaaatgg tgataacaac caagattttg cagccttcaa aggtgttttc
    10561 ttgagaaaat atcaaggctt aaaagcaagt gtctaccatg ctagtgacaa ggaacacaaa
    10621 aatccactat gggtcagccc agaaagcttt aaaggagata aaaactttaa tagtgacatt
    10681 agatttgcaa aatcaacgac cctgttaggc acagcgtttt ctggaaaatc gttaacagga
    10741 gctgaattac cagatgggta ttatcattat gtggtgtctt attacccaga tgtggtcggt
    10801 gccaaacgtc aagaaatgac atttgacatg attttagacc gacaaaaacc ggtactatca
    10861 caagcaacat ttgatcctga aacaaaccga ttcaaaccag aacccctaaa agaccgggga
    10921 ttagctggtg ttcgcaaaga cagtgtcttt tatctagaaa gaaaagacaa caagccttat
    10981 acagttacga taaacgatag ctacaagtat gtctcagtag aagacaataa aacatttgtg
    11041 gagcgacaag ctgatggcag ctttatcttg ccgcttgata aagcaaaatt aggggatttc
    11101 tattacatgg tcgaggattt tgcagggaac gtggccatcg ctaagttagg agatcaccta
    11161 ccacaaacat taggtaaaac accaattaaa cttaagctta cagacggtaa ttatcagacc
    11221 aaagaaacgc ttaaagataa ccttgaaatg acacagtctg acacaggtct agtcacaaat
    11281 caagcccagc tagcagtggt gcaccgcaat cagccgcaaa gccagctaac aaagatgaat
    11341 caggatttct ttatctcacc aaacgaagat gggaataaag acttcgtggc ctttaaaggc
    11401 ttgaaaaata acgtgtataa tgacttaacg gttaatgtat acgctaaaga tgaccaccaa
    11461 aaacaaaccc ctatctggtc tagtcaagca ggcgctagtg catcagctat tgaaagtaca
    11521 gcctggtatg gcataacagc tcgaggaagc aaggtgatgc caggtgatta tcagtatgtt
    11581 gtgacctatc gtgacgaaca tggtaaagaa catcaaaagc agtacaccat atctgtgaat
    11641 gacaaaaaac caatgatcac tcagggacgt tttgatacca ttaatggcgt tgaccacttt
    11701 actcctgaca agacaaaagc ccttggctca tcaggcattg tccgcgaaga agtcttttac
    11761 ttggccaaga aaaatggccg taaatttgat gtgacagaag gtaaagatgg tatcacagtt
    11821 agtgacaata aggtgtatat ccctaaaaat ccagatggtt cttacaccat ttcaaaaaga
    11881 gatggtgtca cactgtcaga ttattactac cttgtcgaag atagagctgg taatgtgtct
    11941 tttgctacct tgcgtgacct aaaagcggtc ggaaaagaca aagcagtagt caactttgga
    12001 ttagacttac cggtccctga agacaaacaa atagtgaact ttacttacct tgtgcgggat
    12061 gcagatggta aaccgattga aaacctagag tattataata actcaggtaa cagtcttatc
    12121 ttgccatacg gcaaatacac ggtcgaattg ttgacctatg ataccaatgc agccaaacta
    12181 gagtcagata aaatcgtttc ctttacctta tcagctgata ataacttcca acaagttacc
    12241 tttaagatga cgatgttagc aacttctcaa ataactgccc actttgatca tcttttgcca
    12301 gaaggcagtc gcgttagcct taaaacagct caaggtcagc tgatcccgct tgaacagtcc
    12361 ttgtatgtgc ctaaagctta tggcaaaacc gttcaagaag gcacttacga agttgttgtc
    12421 agcctgccta aaggctaccg tatcgaaggc aacacaaagg tgaataccct accaaatgaa
    12481 gtgcacgaac tatcattacg ccttgtcaaa gtaggagatg cctcagattc aactggcgat
    12541 cataaggtta tgtcaaaaaa taattcacag gctttgacag cctctgccac accaaccaag
    12601 acaacgacct cagcaacagc aaaagcccta ccatcagcgg gtgaaaaaat gggtctcaag
    12661 ttgcgcatag taggtcttgt gttactcgga cttacttgcg tctttagccg aaaaaaatca
    12721 accaaagatt gataacaaaa cgcaaagaga caacaaatca tcccttttaa cagtcggacc
    12781 tttctttgag attgagctgg cagctgatca tttcttaaca aaagttaaag agttgagcaa
    12841 agtaaggaac aaacataaag accataaaag caagccaaac tggcttgctt ttatggtgtt
    12901 attgtcgtgt gctacgatga ctaaaaaaga acaacgaaca atgacctcga atcaccaaaa
    12961 ggacttgatt tgatagcttc cctatgctat actaagccta ttctttcaag aaggactcct
    13021 tttgtggaaa agactgaaaa aaaggacact aatctatcca aaaactccag atgagtagct
    13081 gcaaacggat gcctttgctg tgatcaaggt gataaagcag actggcttgt cactacctga
    13141 gcaggtatca aaaaaagaag cttttggctt tgtcattcaa catactgagc tttagtattt
    13201 tagaacggag acacccccag ttttgtactc aaaaagcata aacagctctg ctgatgtgac
    13261 atccgatttc tacgtgcgca gtgatccaaa acagctgctg tttgctatgg taaaagtggc
    13321 gccaatcggg atttttgaga ccctaggctc aaaaataagg catagaattc caaaggaagt
    13381 cgctgccgtg agcacaacct aagaaaaact atcccaaaag actttttctg caatttgaaa
    13441 tactgaattt cagtattttt tcttttgtaa ctaagagcaa tgaatgaggt aaaaaaggca
    13501 acaatgtcaa aaaagaaacg aaaacactat caggtttatg agggattacg ctgtgctatg
    13561 acgctttgtt ttatcagtgg ctatgtcaat gcctttacct atatgacaca aggcaaacgt
    13621 tttgcaggtg ttcagacagg caaccttttg tcatttgcca ttcgcttgtc agaacagcaa
    13681 ctgaaggagg cactgcaatt tttacttccc atgatagttt ttatgcttgg gcagtccttt
    13741 acttatttca tgcaccgctg ggccattaaa aaagggctcc actggtactt attatcaagc
    13801 tttatcttaa cagggattgc ttttggaacg gccctattca cgccttttct tccttccaat
    13861 gttacggtag cagctttagc cttttttgcc tctattcagg ttgatacgtt taaaacctta
    13921 cgaggagcta gctatgctaa tgtgatgatg acaggaaata tcaaaaatgc cgcctattta
    13981 ctaaccaaag gcttgtacga aaaaaatcac gaactaacac atatcggacg caatacctta
    14041 atagtcatct tggcttttgc tgttggggtg gtttgttcga cgttgctttg catagcttat
    14101 ggcgagtatg ctttgatgcc tattttaatg cctttactct atgtcaatta cctgttggct
    14161 caggaatttt atcacattca aaccaaaatc aaaccgatta aaagaactta acggacttgt
    14221 ggagaaagaa agttcattta gggtaagttt ttacttaccc tccgttgctt tgctggtttt
    14281 gctgcccctt aaaatatgat ataatagacc attaatagct gttacttaac agaaggctga
    14341 taaaaaaggt tccattagcc ttgagggttg aatttaaaca ggaaaaaacg aaatcctaca
    14401 ctatgcatct caatcgtact ttcatttgag tatggttctg aaaggaaact atgatgaaaa
    14461 aaccatttta cattacgaca cccatttatt acccatctgg gaaattacac attggttctg
    14521 cctatacaac tattgcttgt gatgtcttgg cacgctacaa gcgcttgatg ggccatgagg
    14581 tcttttacct cacaggactt gatgagcacg gacaaaagat tcaaaccaag gcaaaagaag
    14641 ctggtattac acctcaaacc tatgttgata acatggcaaa ggatgtcaaa gccctttggc
    14701 aattgctaga tatttcctat gacacattta tccggacaac ggacgattac cacgaagagg
    14761 tcgttgctgc tgtctttgaa aaattgctgg cgcaagacga catttacctt ggcgaatact
    14821 ctggttggta ttctgtctct gatgaggaat ttttcactga aagccaatta aaagaagtct
    14881 ttagagatga agacggtcag gttattggag ggattgcccc atcaggtcat gaggtcgagt
    14941 gggtctctga agaatcctat ttcctacgcc ttagcaaata cgctgatcgt cttgtggcct
    15001 tctttaaaga gcgacctgac tttatccaac cagacgatcg tatgaatgaa atggtgaaaa
    15061 actttatcga gccaggtcta gaagatttgg ctgttagccg tactaccttt acatggggtg
    15121 tacctgttcc atcagaccca aaacacgtgg tttatgtgtg gattgatgcc ctcttaaact
    15181 acgcgacagc tcttggttat ggtcaagcta atcacgctaa ctttgataaa ttctggaatg
    15241 gaacggtttt ccacatggtt ggaaaagata tcttgcgttt tcatagcatc tactggccaa
    15301 ttttactcat gatgcttgat ttaccaatgc cagaccgctt aattgctcat ggttggtttg
    15361 tcatgaaaga cggtaaaatg tctaaatcaa aaggcaatgt ggtctatcca gaaatgttag
    15421 tagagcgctt tggactggat tctcttcgtt attacctcat gcgttctttg ccagttggtt
    15481 cagacgggac ctttacccct gaagattatg tgggtcgtat caactatgag ttagccaacg
    15541 accttggcaa tttattgaac cgtaccgttg cgatgatcaa caagtatttt gatggcacgg
    15601 tgccagctta tgttgacaat ggaactgcct ttgatgctga cttaagtcaa ctgattgatg
    15661 ctcagctagc agactaccat aagcacatgg aagctgtaga ctacccacgc gccttggagg
    15721 ctgtttggac catcatcgct cgtaccaaca aatacatcga tgaaaccgct ccttgggtgc
    15781 tcgctaaaga agatggtgac aaggctcaac tagccagtgt catggcacac ttggcagcta
    15841 gcttgcgcgt ggtcgctcat gtgatccaac cctttatgat ggaaacatca gctgctatca
    15901 tggcccagct tggcttagcg cctgtttctg acttgtcaac acttgctcta gcagattttc
    15961 ctgccaatac aaaggtcgtt gccaaaggca caccgatttt cccacgtctt gatatggaag
    16021 ctgagattga ctacatcaaa gcccaaatgg gtgacagctc agctatttcc caagaaaaag
    16081 aatgggtccc tgaagaggtt gcgctcaaat ctgaaaaaga cgtcatcaca tttgaaacct
    16141 ttgatgctgt ggaaatccgt gtggcagaag tcaaggaagt gtcaaaagtg gaaggttcag
    16201 aaaaattgct gcgctttaga gtagatgcag gagacgggca agaccgtcaa atcttgtcag
    16261 gtattgccaa attttacccc aacgaacaag aattagtggg taaaaaacta caaattgttg
    16321 ctaacctgaa accgcgtaaa atgatgaaaa aatacatcag ccaaggcatg attctatcag
    16381 cagagcatgg agatcaacta acggtcttaa ccgttgattc ctctgttcca aatggaagta
    16441 tcattggctg atgctgacct attattcaaa aaacctaagc aagaacatgt gcttaggttt
    16501 ttttgattgc cttcaagaag gacgaataac gagatgggtg atatggcatc aaaagcataa
    16561 ctatcctagg acctgtccta ggatagttat gcttttgaca agtgaacaga gaggtcttaa
    16621 aactatatct tgtcataaaa aagattgcaa aacactatat cttgtgttat tatggttttt
    16681 ataaaagtta agtatgctta actttagcca aacaaaagag gacactagag cgtgtcccac
    16741 aagacagttg agcccttcgc ttttttagta gagtaggagt gctcaagtca aaaagagaca
    16801 tcccatcaaa ccattgctgt ttagcaggtc tttttgtgtt gctcaaaaaa aacaattcct
    16861 gtctgtttgg cgtctagtga tgacttaggc ttagcgctac tagacttttg tttgaaaaat
    16921 acctcaagcc ttgaaggaga caaatgaccc aacattatta tgaacgttcg caatccccaa
    16981 ttgaatacgc cctatctgaa acacaaaaac agttgcgctc catcaattgg aattacctta
    17041 atgatgacaa agatttggaa gtgtggaatc gtgtcactca aaatttttgg ttgcctgaaa
    17101 aggttcctgt ttctaatgat ttaaattctt ggcgctcctt gggtgaggat tggcaacagc
    17161 tcatcacaag gacctacact gggttgacct tactagatac agtacaagca acggttggtg
    17221 atgtggcaca aattcagcat tcacaaaccg accacgaaca agtcatctat acgaactttg
    17281 cctttatggt ggggattcat gcccgctctt acgggacgat tttttcgacc ctttgcagca
    17341 gcgagcaaat tgaagaagca catgaatggg tggtctcaac acagagcttg caagaccgtg
    17401 cccgcgtctt gattccttac tatacgggtg atgacccttt gaaatcaaaa gtagcagcag
    17461 ctatgatgcc aggatttttg ctgtatggcg gcttttacct tcctttttac ctatcagcac
    17521 gtggtaaaat gccaaacaca tctgatatca tccgcctgat tttacgcgac aaagttattc
    17581 acaactatta cagcggttac aaataccaac aaaaagtagc acgtcttagc ccagaaaaac
    17641 aggctgaaat gaaggccttt gtctttgact tgctctatga actcatcgac cttgaaaaag
    17701 cttacttgag agaactgtat gcaggctttg atttggcaga agatgctatc cgctttagcc
    17761 tttataacgc tggaaaattc cttcaaaatt tgggatacga gtcgcccttc acagatgaag
    17821 aaacgcgcgt ttcaccagaa gtttttgctc agctatcagc gcgtgcagac gaaaaccatg
    17881 actttttctc aggcaatggc tcatcttatg tgatggggat caccgaagaa accacagatg
    17941 acgattggga gttttaaaaa atggcagagc tgattattgt gtatttttca tctaagtcca
    18001 ataacaccca tcgcttcgtc caaaaattag gcctccctgc tcagcgcata ccagtagata
    18061 ataggccact tgaagtttca acccattacc ttttgattgt gcctacctat gcagctggtg
    18121 gctcagatgc caaaggagcc gttcctaaac aagtgattcg ttttttgaat aatcctaaca
    18181 atcgtaaaca ttgtaaggga gtgatctctt caggaaatac caattttggc gatacctttg
    18241 ccttagcagg ccctatcatt tctcaaaaat tgcaggtccc cctattacac caatttgaac
    18301 tattaggcac tgctacagat gtcaaaaagg tccaggcgat ttttgcccgc ttaaagcatc
    18361 acacacacga caagcaaaaa caaaccaaca acctgattac agaaaggaca cacccatgtc
    18421 acaaaccaat gcgtcatacc tctcattaaa tgcactgact cgttttaaaa aaccagacgg
    18481 cagctaccat tttgatagcg ataaagaagc tgttaggcgc tacctggaag aacatgtctc
    18541 acccaatcaa atggctttta actcactaga agataagcta gcttacctca tcaatgaagg
    18601 gtattatgag caggccattt ttgatgccta tcctaacgac cttatcaagg aggcctttca
    18661 ttatgcctat caacaaggct atcgcttttt aaatctcatg ggcgctatga aattttacca
    18721 gtcctatgcc ttaaaaacct tggacggtaa gcagtatcta gaaacatttg aagaccgtgc
    18781 tgtgatgaat gcactctttt tagctgatgg agatcaaacc tttgtctttg acgtgattga
    18841 tgccatcttg caccgacgct ttcaaccagc caccccaacc tttttaaatg ccggaaaaaa
    18901 acgccgtggc gaatacattt cgtgctatct tcttcgtgtc gaagacaata tggaatctat
    18961 ctctcgcgcc attagcacca gcttgcaact ctcaaaaaga ggaggcggag tcgccttgtg
    19021 cctcaccaac ctcagagaaa tcggagcacc gataaaaggg attgaaaatc aggcaacagg
    19081 catcgttcct gtcatgaaac tactagagga ctctttttcc tatgccaatc aactaggtca
    19141 gcgccaagga gcaggtgcgg tttaccttca tgcccatcac cccgaggtcc tgaccttttt
    19201 agacaccaaa cgtgaaaatg ccgatgagaa aattcgcatt aagtccctag cattgggact
    19261 tgtgatccca gatattacct tccagttagc caaagaaaac aaagacatgg ctctcttttc
    19321 accctatgat atcaagcgtg tctatggcaa ggacatgtca gacatctcta tcacagagga
    19381 atacgataaa ctgctagcca atccagccat caaaaagacc tacatcagtg ctagaaagct
    19441 ctttcagtta attgcagagc tacattttga gtcaggctac ccttacctct tgtttgatga
    19501 cacggtcaat aagcgcaacc ctcatgccaa aaaaggcaga atcgtcatgt ctaacctgtg
    19561 ttctgaaatc gctcaagtca gcaccccaag cactttcaaa gaagatttga gttttgaaac
    19621 cattggagaa gatatctgct gtaatttagg gtctatcaat attgctcagg ctatggcaga
    19681 tgcgccacac tttgaacagc tcatcacaac aagcatcaga gcacttgatc gtgtctctag
    19741 agtgtctgac ctgaattgtg cgccttctgt tgagacaggc aatgcagcca accatgcagt
    19801 aggattaggc gccatgaact tacatgggtt tttggcaacc aaccacatct actatgatac
    19861 aaaagaagcc gttgacttta cagatctgtt tttccatgcc atggcctatc atgcttttaa
    19921 agcctcttgc caactagcca aagaaaaagg ggcttttgca ggattttcgt tatcaaccta
    19981 tgcagatggc acttattttg ccaagtattt gcaagaagat gccaagccac aaacagccaa
    20041 ggttgccaca ttgttgcaag attacggctt cacattgcca acggtggcgg actggcaagc
    20101 cctagtagca gatatcaagc agtttggcct agccaatgca cacttgctag cggttgctcc
    20161 gacaggctcc atcagctact tatcatcatg cactccgagt ctacaaccag tcgttgctcc
    20221 tgttgaggtc cgaaaagaag gtagcctggg caggatttac gtccctgcct atcagattga
    20281 ccaggctaac tatgcttact atgaacgtgg tgcctatgag gtcggcccaa aagccatcat
    20341 cgacgttgtc gctgctgccc aaaaacacgt tgaccaagcc atctcattga ccctgtttat
    20401 gaccgatcaa gcgaccacac gtgaccttaa ccgctcctat atccaagctt ttaagcaaaa
    20461 ctgtgcatcg atctattatg tgcgagtgag gcaagacgtc ctagcaggca gcgaacagta
    20521 tgacgaggat agtcttgtga cagctcctgg agctagtgat gagaccacca cagagtgtca
    20581 tagctgtatg atctaacagc tactagaaag ctctcgtgtc ccctaccaca tgcataggat
    20641 cagtgcgctc gacctttcaa gacaagcaag catcagctct tgcttgtctt tttttggcct
    20701 caaagcccgt tagtctgctg ctatgcgagg ctttttttga gcatcagaac ctcaaaaaaa
    20761 ggacatggag tcctttttgg ggtgatcggt gttgaggcta tcacaaactg cccttgaaat
    20821 acgcttctat gtggagcttt ttgtggtcct gtgacacgta agctccaacg acctccagct
    20881 gacagcctct tggaaacaag agctcaacct ctgaaggcac agccgaataa ggctcgacaa
    20941 aggctgcctt ggcccctttt ttgacacaga tgcgcacctc caccggtcga tgggtcatag
    21001 cgccgttttt caaggctgtc gtgctcataa aactgtgctt agtgtagcgt gtgccaagct
    21061 ttatcttaca aaggatatga gggtcaaact gatggttacg gtagtaagac gtgagatcag
    21121 cgtgtgaaac accgatatca cgcaaaaacg tctcgtatac atagcggtat accacaatgt
    21181 tccacgggat gactagccgg tgagtcgcag catcgagctg ggccacctga tcccttagct
    21241 caggcgtgag ttggctgagc tcacccttgg tcttgtctag gctggtattg ataagggctg
    21301 cctgctgaca ggtataagca gccatcgcct cttgctcagc ccttgggtag aggagaccct
    21361 tgccccaacg ctttttaatc agtgtctcag cgcccttttg atccgaaaag gtaaaataag
    21421 caggcgtccc ctcacaaacc gcccgctcat agtgctcaca gacaaaaagc tcgactaagc
    21481 cctcagtttg ccagaccaga ctaaaacagc taagaagaag gatcatagcc agttgatagc
    21541 gcttttttag catagttccc cctgacacca gcccaaaagg ctgacccttc ccctaggcct
    21601 tttggctagc tcatgatacc accagtttct cagacaggct aatctttttg tcatctcttt
    21661 ctccctaaac agactctctt atcatgatat tatgctatta tgagcgattt gtcaatccat
    21721 agcaaccgtt ttttagttag ataagcaagc ttttgaccta gaggcagaca agagtggcag
    21781 tccctaccgt ttgctgtggt ggctaatacg gactaacaca aaatctcaaa cgctttcaaa
    21841 aacagctcct gagcttaaaa aaggctgatg aacagatttg ttgaacttaa gagagattgc
    21901 catgattttc taatcatttg atgtttaaaa tgataaaaac atgaacttga aatgaaagaa
    21961 tgcgagtagt aaactataac cgtatcaaaa aataatgaaa tggagtgatt ttatgaaaac
    22021 aaaatcaaaa cgctttttaa acctagcaac cctttgcttg gccctactag gaacgacttt
    22081 gctaatggga cgtcccgtaa atgcggaggt gacatataca ggtgagagat caacatcagt
    22141 gaatggtcct ggtgaagata gtagcagtga gaaaacggaa caaactagtg atagtgacaa
    22201 aagagatcgg aatcgtggtt atgaagaggg ttataagcgc ggacaagagt cagataatcg
    22261 tgttctagat agagggacta ttgaggttcc ctacaatgta atagacggag ttgaatataa
    22321 ggatggctat gaacagggat tcgcagcagg atggtacgat ggtcatccaa tcacagcact
    22381 actagacata gcttggtcct acttaacata tgcatttgat gttttgtttg gtggtacaaa
    22441 ttcttagtaa atggcgtatt tgttgtctta atgtagttat tgtgacttat tgaaaagctt
    22501 gaggatactt tgcttagctc aagcttttcc cacacgaatc tgttataatt aaattattta
    22561 gttgccacgc tatctttgat aacgtggctt ttcggtgtag gtgaggttat caggatggat
    22621 ttattttcgg atcttaatag gagacaacat gaagttacag atcgtaaaga agacgctaga
    22681 gtaaacagtg actctaattc agacaaatca aacagattta gtatcttacg atttatttct
    22741 tgtttacgta atttctggag ttgtcaaata tacctgaagc tgtgtattac agtggttcta
    22801 cgacgacgct tgatcttgag ctttacgact tatccgtgaa taggtatatt aaccaaatta
    22861 attttaagta ataaggagag atactttaaa tgacaaaagc aataaagttg gttattctca
    22921 gtttgattct atttactaca gtttttcttt cagttagaca agctagtgct gatcttatga
    22981 tggatggagt tacttattct gaaagtacag atagtaatac atttttatca aactctggag
    23041 aggtagataa catatgggaa aaggatagag attttggaaa acaacgagga cttgaggctg
    23101 gtaaaaatgg acttggccca acaataaatc gaggtgatat accagttcct gaaaaggtaa
    23161 aacataaaga taaatatcag gatgggtatc aagaaggtta tatccaggca tggcatcagg
    23221 agcatccatt tcaaggagtt atattcgctg tattcgatgt ttttagtttg atatggggtg
    23281 taataagtga gcttttcact tgagcgaatt agttatagtg acgctattag agagtaatat
    23341 tatctgttga aagtactgta atgtttaggc tacagcgcta tttttggtac atatagctaa
    23401 aagtactaca gttaattttt tatcacaagc cattcccaca caaatctgct ataattggga
    23461 tagtcccttt agtagtcagg ccacctctga taggggtggc ttttttgggt tttagtgacc
    23521 cgtgtgttga gaaaggagca tgtatgccag aaaagaaagt gttgcccgtc ctaagcatgg
    23581 tggtgagcct tgtgtctctg tttgatcata tcttcttttt tgctacttat agttatctct
    23641 acttgccgct tgtagcaagt agtctagctc ttatcggtct ggtgcaaaac aaaggcaggc
    23701 aaaaggtttg gtcgctagct gggcttatcc tagctgctct agccgtggca gtagtggcct
    23761 atgcctacta tgacccaacc gtcatcccct actaaccttc ccccctcttt gcacgcaatc
    23821 agcaacacaa aaagcacccc tgacaggtgc tttttgtgct ttagccaacc tcatagccta
    23881 gtaaatcctc actgaaacag tcccttaaag ttccaccact gatcttggat gctttcgctt
    23941 aaccgctgat gccaaggttt atggtcttct tctcttatct gctttagcat gctttcttct
    24001 agctgttttt gttgcttttc ctcttccttt ttttgaagtg cttcttcagc ttctttttct
    24061 ttttttatag tatcagagtc tctatagata taactaccga cataaaatcc gtcttctcct
    24121 ttaaatgaga aagttaattt ttctcctttc ttcaaaggat cttctcctaa gttaagaata
    24181 taaggtactc ctgtataata ttgacgataa ggatctgttt ttgtcatatt tttttcgaga
    24241 ataggactag ctaaaattcc tcgttcttta ctgttatgat cacattgtct gtaaagatat
    24301 acccaccatc cttgagggat atatattttt actactttac taccaacata taagtcttct
    24361 agagtgattg tgttgccact ataatcttta attttttgtt gaggttcatc caaatcaaat
    24421 tttactggct tttgtttact actttgttca tcctccgctc tgacgctact agttaccccg
    24481 atggcaaaga gtgccagtag caaggttaat gtctttttca ttttttcctc tccttttcct
    24541 cattttttaa gacaagggtt attataccaa ttcttgcgct aaaaagatag agagtttatg
    24601 tcttgtggtt gggaaatggc gcgactattc ttggtagggt caaacagggt gactaaagtt
    24661 cctattagcg caacggatat cgtccataaa ggagaacaat atgacatttg aagagattct
    24721 cgaccctaga tagttactga ctttcatagg gtttcttatc tagtagtatt gaggatttta
    24781 tcacacaaat cgtttcatag acttgtttta aaagccatca caagtccact aaaaagggga
    24841 taaaaaaatg cgtccgcagg caagaaaaaa ggcaccttac ggggtgtctt ttaattaatt
    24901 tgtcaataat aattgacgtt tattttgtaa gtggtataat atagttaata aaaaactcgt
    24961 gaaggataaa cgctgggtcc caaaatgggg taggtactta gtactgagca ttcctatgtg
    25021 cctggggttt ttttatttgt tcgatttttt aaggctctca acaaagtttt gagggtcttt
    25081 ttttatttca tttactataa attcaacaaa ctgttgtgaa tatgtataag tctgtgttga
    25141 tcctataaga tgacaatatg aaaattttgt ttcactctta atattataga aatcaatcac
    25201 taaatttaat acataagtat tgaacccttt ttcatagcca agttttatcg cagacttttt
    25261 tagcctttcc tgaactgctg ttactacatt gctataggaa tatttatgag tatctgacgg
    25321 atcttttaat tctttaacta gtgaaacagg ggtttcagca tcttttgaaa tactaacaac
    25381 gaagtcagcc tcatcttttt tcttagtaat ataaagattt tgtctaatat caattgaaaa
    25441 tttttcagaa ttatactctt tactcagtac atcaacttca ttagcttgtt tgatgaattt
    25501 ttctgcaatt tctgaaggat attttagctt tatttcctca ttggataaag gttcgtaact
    25561 tgctgatatt gttaaaaaat tatgtgctat tgattcggta atatctttat tatggaatct
    25621 tttcatttct gacgctaaat ttaaaacgca tgcttgaaat aatggagcat atttaatttc
    25681 atagtcttca gtaataaagt gggtgctaat atttcttaat tcaattattt tttcaagatt
    25741 taaccgaatt ctggtatttt catcagaata tatctttttt aaaacatatt ctaggctcaa
    25801 tgttctttct ggattatcac tgtaataaat actaaaccct cggttaagca tctcagcttt
    25861 tagcattagt tcccaagcat tacatataaa aaaactaaag ccttcaattc gatattttat
    25921 tgtcggttta ttatatattt cgagccccat aataaaagct tcgacacttt ttaaaactaa
    25981 tctttcactt aaattttcca tttaattact ccgtcaaggt gacttttatt atttaagtgg
    26041 cttaatgaat tggttaaaat ctttttagtt tagtaaaatt gctatttaag gttagccacc
    26101 tctcgtatgg tcacagttga tgaattatgc tatagtatag acataaaaaa tagttcaaat
    26161 cccttcttgc ttgttggtgg attttgaact gaaagcctct ctgattgcag ttggtaggct
    26221 tttttgatgc tttttcgtac tttttatatt tattacgcta aaaaatgtta tacttgatga
    26281 agaaaaagga gagtttagaa cgttaagata cctcacagca gattgataga ggatatggaa
    26341 gaaggttagc tttcccaaga gatgccgttt gggaatggta taacaataac tggcagaagt
    26401 tataagtaaa acaagaacaa attaactcag atgattttta gacgttccca cgcgtgtgcg
    26461 aatgcgtgtg cgaattgatg ttatcaataa atcctttaat aacgcctttt tcgaacagga
    26521 aacaaattta ttatcccacg cgtgtgcgaa tgtgtgggcg aattaattgg agaaaaaatg
    26581 agaaaatacg atatacaaaa aaggagaaca atatgacatt tgaagaaatt ctaccaggtt
    26641 taaaagccaa gaaaaaatat gtacgtacag gttggggtgg cgctgaaaat tatgtgcagt
    26701 tgtttgatac ccttgaagtg gatgggaaag tactacaggc gacgccttat tttctgattc
    26761 atgttactgg tgcaggtgaa ggtttttcta tgtgggcacc gacaccatgt gatgtgttag
    26821 cagaagattg gatcgaggtc aatgactaaa gttgttttag tgacgggctg tgctagtggg
    26881 attggttgtg cacaggcccg ctattttttg agacaagggt atcacgtata tggtgtggat
    26941 aaatcggata aaccggactt aaacggtaat tttcacttta tcaaattgga tttatcctct
    27001 gagttatccc cactatttaa agtggtcccc agtgttgata ttttgtgtaa tactgcgggg
    27061 attttagatg cctacaagcc acttttagac gtcagtgatg aagaagtgga acacctattt
    27121 gatattaatt tttttgcgac ggtgaagtta acacgtcatt atcttaggcg tatggtggaa
    27181 aagcagtcag gtgttatcat caatatgtgc tcaatagcaa gttttatagc gggtggcggt
    27241 ggtgttgcct atacgagttc taagcatgcc cttgcgggat tcacgcgcca gctggctttg
    27301 gattacgcca aagatcagat tcacatcttt ggtatagcac caggtgctgt caaaaccgct
    27361 atgacggcta gcgactttga gcctggtggc ttagcagact gggtagctcg tgagacgcca
    27421 attggtcgtt ggactgaacc agatgaagtt gctgaattaa ccggatttct tgcttcagga
    27481 aaagcgagat caatgcaagg cgagattgtc aaaattgacg gtggctggag tttgaaatga
    27541 tcacatgcct tagcttgagc tggcgcagta agccctagag gtgtgatgag tcaaacagtc
    27601 aaaggagatg aacaaatgaa attagcggtt ttggggacag gtatgattgt taaggaagtt
    27661 ttgcctgtct tgcagaaaat tgatggtatt gatttagttg ctatcttatc aacagttaga
    27721 agtttaaaga cagctaaaga cctcgctaag gcatatcaca tgcctctagc tacgagtaaa
    27781 tatgaggctg tcttaggcaa tgaggagatt gacacggtct atattggact gccgaatcac
    27841 ctgcattttg cctatgctaa ggaggctctt ctagctggta aacatgtcat ttgtgaaaag
    27901 ccctttacca tgacagcagg agaattagat gaactggtgg taatcgctaa aaaacgaaaa
    27961 ctcattttgt tagaagcgat tacgaatcag tacctgagta acatgacctt tatcaaagag
    28021 catcttgacc aacttggcga tatcaaaatt gtagaatgta actattcaca atattcttca
    28081 cgctacgatg ccttcaaacg aggtgatatc gcgccagctt ttaatcctaa aatgggtggt
    28141 ggagctttac gtgatttaaa catctacaac attcattttg tggtaggatt atttggtcgc
    28201 cctaaaacgg ttcaatattt agccaatgtc gaaaaaggca ttgacacatc aggcatgctt
    28261 gtcatggact atgagcagtt taaagtggta tgtatcgggg ccaaagactg cactgcagaa
    28321 atcaaatcaa ccatccaagg taataaaggg tcactggcag tgcttggagc aacaaatacc
    28381 cttcctcagg ttcagttaag tctacacggt caaaaaccac aagtgattaa ccacaacaag
    28441 catgaccacc ggatgtatga ggagtttgtc gcttttaaag acatgattga ccaaaaagac
    28501 tttaagaaag tgaatcaggc tttggagcat agccgagctg tcatggctgt gttggagcga
    28561 gctgttcatt cgtgattgta acctagtaac cattgccttt tgtgcaggtg ctatggtaat
    28621 ggttgaaaga cgtgttttca cgagggttaa ccccttcgtg cttttttggc aaaaataact
    28681 tgttcaggat ttgggctttc tttgtgagat gagagatgcc tgacgaattg atttgattaa
    28741 aataatgata aagccaaacg atttaagaaa tgaccgttcg gaaattctgc cccaaaaggt
    28801 ctctagaagc agtaaaatcg aatttgtaag cgttgacact gttgcataac tctgttacac
    28861 taagagaatg tcatgacaaa aattacagtg taggagttta attgtttatg gaagaggatt
    28921 ataacaaaag agaacctgag aagtttacgc aatttctgcg ccgtcaaaag gtcgtctttt
    28981 ttgtcgcatt ttttggttat gtgtgtgcct acttggtacg caacaatttc aaactaatgt
    29041 caaacactat tatggtgcaa aatggttggg acaaggctca aattgcgacc ttgttatcgt
    29101 gtttgaccgt ttcttatggt ttagcaaaat tctatatggg ggccttaggg gatcgtgtta
    29161 gcttgagaaa gctcttttcg atcagcttgg gtgccagcgc tttgatttgt attttaattg
    29221 gttttttcaa tagttcaatg gtggtattag ggatactgct agtcttgtgt ggtgtcgtac
    29281 aaggtgcctt ggcacctgcc tcacaagcca tgattgccaa ttattttcca aataaaacac
    29341 gcggcggagc catcgctgga tggaacattt ctcaaaacat gggatcagcc ctcttgccat
    29401 taaccatcgc cttgctcacc agtatgggct tggtggtacc agctaatggc aatattttac
    29461 ttgccttttt aatccctggc gttttagtgt tcttgtttgc tttgtgttgc tggaagcttg
    29521 gcggtgataa ccctgagtct gagggacttg actctcttcg aacgatgtat ggtgatgcgg
    29581 gcgagtcagc tgttgccagt gaagaagaaa agcataacct gtcttattgg caactcatct
    29641 ggaaatacgt tttttgtaac ccgtcgctct tacttgttgc tgctgtcaat gtggcccttt
    29701 attttgttcg ttttgggatt gaagactgga tgccgatcta cttgtcacaa gtagccaata
    29761 tgtcagaggc tcatatccat tttgcgattt caatattaga gtgggtagct attccaggct
    29821 cgctggtatt tgcgtggtta gcggttcgtt atcctaataa aatggccaag attggagcta
    29881 ttggactttt tgtgttagcg gctattgtct ttgtctatga acgcttgact gccacaggtg
    29941 ctccaaacta tttcttgttg cttgttattg caggtatttt agggtcattg atttatggcc
    30001 cacagttgat cgtgaatatt ttaacaatca actttgttcc tttaaatgtt gcaggaacag
    30061 cgattggttt tgtaggagta acagcttatc tcatcggcaa tatgggagca aactggctga
    30121 tgccgatttt ggcagatggt tttggctggt tttggtcata tattgtcgtt gcagccttat
    30181 ctgctttttc agcggttggt tatttgattt tagccaaacg tgaggaagaa atcatcaaag
    30241 attagctagg caaatgccta agaaagacag acttattgca ataagtcctg tcttttttag
    30301 tcatcacaag tcgcaagtga cactataaaa gacaagaaaa tgctaggatg tttcaggaaa
    30361 aaaagagaaa atatgctata atgagaaggt atttaaatta tatagtaagg aaaaacaaat
    30421 gacaaattat gccattattt tagcagcggg caaagggact cgcatgactt ctgatctccc
    30481 aaaagttctt cataaagtat ctggactaac catgcttgag catgtttttc gtagtgtaaa
    30541 agcgattagt cctgaaaaag ccgtgacggt tattggtcac aaatcagaaa aggtacgtgc
    30601 tgtcttagca gatcaatcag cctttgtcca tcaaacagag cagttaggaa ctggccatgc
    30661 cgtgatgatg gcagaaacac aactagaagg actagaaggg cataccttgg tgattgcagg
    30721 agatactccc ttgatcactg gagaaagcct gaaaagtctg atcgattttc atgtgaatca
    30781 taaaaatgtc gccactattt taacagctac ggctcaagat ccatttggtt atggtcgtat
    30841 tgttcgcaac aaagatggcg aagtgatcaa gattgttgag caaaaagacg ccaatgagta
    30901 tgagcaacaa ttaaaagaaa tcaatacagg aacctatgtg tttgataaca agcgtctctt
    30961 tgaagcgctc aaatgcatca ctaccaacaa tgctcaagga gagtattacc tgactgatgt
    31021 tgtggctatt tttagagcaa acaaagaaaa ggtaggagcc tatatcctga gagacttcaa
    31081 tgaaagtctt ggtgttaatg atcgtgtggc cttagcaaca gctgaaacag tgatgcgcca
    31141 acgcatcacc caaaaacata tggtcaatgg ggtgactttc caaaatcctg aaaccgttta
    31201 tattgaaagc gatgttgaga ttgcaccgga tgttttaatt gaaggaaatg tgaccttaaa
    31261 aggacgtaca catatcggct caggaactgt cttgacgaat ggtacttata ttgtggattc
    31321 tgaaattggt caaggaagta tcatcaccaa ctcaatgatc gaatcatctg ttttagctgc
    31381 aggagttacg gttggccctt acgcccacct tcgaccagga acgaccttag gtagggaagt
    31441 ccatatcggt aactttgttg aggtcaaggg ttctcacatt ggtgaaaaaa caaaggctgg
    31501 gcatctgact tatattggaa acgctcaggt tgggtcttca gtcaatgttg gggctggaac
    31561 catcactgtc aactacgacg gccaaaacaa atatgaaaca gtcattggag atcacgcttt
    31621 tattgggagc aactcgactc tcattgcacc tttggaagtt ggagataatg ctttaactgc
    31681 agctggctca acgatttcta agacagtacc agctgatagc attgttattg gtcgtagccg
    31741 ccaagtcaca aaagaaggtt atgccaagcg cctagcgcat cacccaagtc gaagcaaata
    31801 attcaagatc tatttattag taaggaggcc aggcatgaaa tttgaagaaa aaacgcttaa
    31861 gagacaaaca gtttttgatg gtcatatttt taaagttgtt gtggatgatg ttgaacttcc
    31921 caacaatctg gggcaatcca agcgagaatt aatttttcat cgtggtgccg ttgctgtttt
    31981 ggctattact cctgaacgta agattgtcct tgtcaaacaa taccgtaagg ctattgaaag
    32041 tgtctcatac gagatcccag cgggtaagct agagattggt gaggaaggct caaagctaaa
    32101 agctgctgca cgtgagctgg aagaagaaac agcctatacg gggacattga cgtttttgta
    32161 tgaattttat acagcgattg gtttttgcaa cgaaaaaatc acacttttct tagcgacaga
    32221 cctaacacaa gttgccaatc ccaaacctca agacgacgat gaagttatcg aggtcttaga
    32281 gttgacatat caagagtgca tggacttagt cgctcaaggt aaacttgctg acgctaaaac
    32341 gttgattgct ttgcaatact atgccttaca ttttggaggt gagcagtgat gggaaggcca
    32401 ttgttgaccg atgatatcat cgaaaaagcg agacgaatgg aaacatttga gccagatgat
    32461 gctgtagact ttgacacaaa agtaatgacg ctaccagaaa aagatgaaaa agcacgcatt
    32521 tataaaagcc gtcggattga aaatgccaaa cgcagccaat tgcaatctaa attaaatgtg
    32581 attttgatag ctgttatgct gttaattgcc atcttggtgt atgctatctt ttatctttaa
    32641 aaaaacgtaa ggaaatgata tgaaaatagg aattattgct gctatggagg aggagttgtc
    32701 cctgctctta gcaaatcttt tggatgctca agaacatcag gtgctttcaa aaacttacta
    32761 tacgggtcgt tttggcaagc atgagctcat tttggtacag tcaggtgttg gaaaagtgat
    32821 gtctgctatg acagttgcga tcttggctga gcatttcaaa gcccaagcta ttatcaatac
    32881 aggatcagca ggggcagtcg cttcacattt agcgatcgga gatgtggtgg ttgcagatcg
    32941 gttagtgtat catgatgtgg atgctactgc atttggttat gcttatggtc aaatggctgg
    33001 tcaaccatta tactatgatt gtgatccaca gtttgtggct atcttcaaac aggtcctaaa
    33061 acacgaaaag acaaatggcc aagttggttt gattgcaaca ggagacagtt ttgttgctgg
    33121 tcaggacaag attgaccaga tcaaaacagc ttttagcgac gtgttagcag tagaaatgga
    33181 aggcgcagct attgctcaag cagcacatac tgcaggtaaa cctttcatcg ttgttcgtgc
    33241 gatgagtgat accgcagcgc atgatgccaa tattactttt gatcaattta ttatagaagc
    33301 tggcaagcgt tcggcccaga tattaatgac atttttagaa aacttacctg tttaactcac
    33361 cttatcgtga gacaattagc tggataatct ctattttagg agtctggtca cctcttgttt
    33421 tgaaacctaa aaataagaat tgcaatcact tgaccaaacg ctagcatttg actggtccaa
    33481 aatcacacct gcaatatagt aaagacaaca ccaaacttca attgagaaat atccttaacg
    33541 gctaccaaag tgacctaggg agacactact ctagctatta ttactacaac ctaagaaccg
    33601 ttatgggact atcaagtgag caagagattg aagaacacta tgaaaagctt aagaacaagt
    33661 tacatgatat gtacaatcat tattaagtca aaggcttaaa aatggcgact agcccctcaa
    33721 aaagaggcca cactcattta aatttaaatg agtgtggcct ctttttgagg aggcgcaagg
    33781 ggttaaaggg ctgtgacata aagttgctta gcaatatttt cagggatgac aagttccttg
    33841 tcgccataac aaatcgtata ggtttttgca aaggtatcaa tttgagttaa ggttagctcg
    33901 gtattgatac tcaggtgatg tgcttctaga tactggatta aatcaaagtg gtcgtggatt
    33961 ctgcttaggc gaaaccgtcc caactccgtg atggtgttga gagttgtagt gttcatttcg
    34021 acaagaggct gaccatagcg aggaatagtc cctccgtgtg ggcagaaatc tggaaaatca
    34081 aggatcttgt ctaaacggtc gataaaggtg tctgagacag tgtgctctaa gacttcagct
    34141 tcttggtgca cttcttgggt attatagccc agctggtgaa tcaaaaatac ctcaatcaag
    34201 cggtgttttc ggtagaggtt agcaacaaga gcatagcctt tgtcttttag caaatagcct
    34261 tttgctttat ctttgacaat ccagccttgt gaaatcattt ttttaatcat ctctgaaacg
    34321 gcaggtgccg aaacgtgcat cttttctgca accattttat ttgtaatctt gggttcttgt
    34381 tctccaattt catagataca tttcaagtaa tcttctttat taggcgtcat atcgtttggc
    34441 ctttctctta tttataatga ttattatatc agaattttta ggaaaaagct tgacaaaaag
    34501 cctaaaagag gatatcctta tagcataatt aactaaactt aattaaataa ataatatatc
    34561 ctaaggagag aaatgatggg aaagagaatg agcctcattt tgggtgcttt tttatctgtt
    34621 tttcttttag tagcttgttc gtcaactggc actaaaactg ctaagagtga taaattaaaa
    34681 gtcgtggcaa ccaattcaat tattgccgac atgacaaaag ctattgctgg tgataaaatc
    34741 gatctgcaca gcattgtgcc aatcggtcaa gaccctcatg agtacggacc attaccagaa
    34801 gatgttgaaa aaacaagtaa tgctgatgtg attttctata atggtatcaa tctagaagat
    34861 ggtgggcaag cttggttcac caaactagtg aaaaatgctc aaaaaacgaa aaacaaagat
    34921 tactttgccg tgtctgatgg cattgatgtg atttacttgg aaggtgcaag cgaaaaagga
    34981 aaagaagatc cacatgcttg gttaaatctc gaaaacggaa tcatttattc aaaaaacatt
    35041 gccaaacaat tgattgcaaa ggatcctaaa aacaaagaaa cttatgaaaa gaacctaaaa
    35101 gcttatgtgg ctaaattgga aaaactagac aaagaagcca aatcaaaatt tgatgctatt
    35161 gcagaaaata aaaaattgat tgtgactagt gaaggctgct tcaagtactt ttcaaaagct
    35221 tacggtgtcc catctgctta tatctgggaa attaacaccg aagaagaagg aacaccagat
    35281 caaatttcat cattgattga aaaactaaaa gtcatcaagc catctgcgct ttttgtagag
    35341 tcaagtgtcg atagacgccc tatggaaact gtttctaaag atagtggtat tcctatttat
    35401 tctgagatct ttacagattc aattgctaaa aaaggtaaac ctggcgatag ttattatgct
    35461 atgatgaaat ggaaccttga caaaatttcg gaaggtctag caaaataaga taacaataaa
    35521 gcacagcgat tttagtgttg tgctttgttt tatttatgta aaggagacaa aatgattaca
    35581 actaacaatc tttgtgtgac ctacgatggc aacagtaatg ctttagaagc gattaatgtg
    35641 acaattgaag gtccttctat cgttggtatt attggtccta atggagctgg aaaatcaacc
    35701 tttatgaaag ctatcttaaa tcttattgat tatcaaggtc acgtaacggt tgatggcaaa
    35761 gatggtcgta agcttgggca tacagtagct tacgtagaac aacgaagcat gattgactat
    35821 aattttccta ttactgttaa agaatgtgtg gcgctaggga cttatagtaa attaggcctt
    35881 tttcgccgag taggaaaaaa acaatttgag caggttgata aggttctcaa acaagttggt
    35941 ttagaagact ttggccatcg accgataaaa tccttatctg gtggtcagtt tcagcggatg
    36001 ttggttgcgc gttgcttgat tcaagaatca gactatattt tcttagatga accgtttgtt
    36061 ggcattgact cggtcagcga aaaaatcatt gtagatcttt tgaaagagct caaaatggct
    36121 ggcaaaacga ttttaattgt acatcatgat ttgagcaaag tagagcatta ttttgacaaa
    36181 ctcatgattt taaataaaca tttggtggct tatggcaacg tttgtgaggt atttactgtt
    36241 gataccttgt caaaagctta tggcaatcat ttgattttag gaaaggagat ggtgtaaatg
    36301 tctatgaaat tttttgaggg cttgatgtca taccactttt tacaaaatgc actgataacg
    36361 gctgtagtca ttggtatcgt ctcaggtgct gtagggtgtt ttattatcct taggtcaatg
    36421 tctcttatgg gtgatgccat ctcacacgct gttttaccag gggttgcttt gtcatttatt
    36481 ttaggagtca atttttttat tggagcgatt atttttgggt tattagcttc tgttattatt
    36541 acttatatca aggaaaactc tgtcattaaa ggagatacgg ctattggtat cacctttagc
    36601 tcttttttgg cactgggagt catcttgata ggggtagcca atagttcgac ggacttattt
    36661 catattttgt ttgggaatat tttagctgtg caagatagcg ataagtggat tactattggt
    36721 gtttcgattt ttgttttagt ggttattagt ctctttttca aagaactatt attaacatca
    36781 tttgatccta tcttagccaa atcaatgggt gtgaaggtca atgcgtatca ttatttgttg
    36841 atggtcctat taactttagt ggctgttacg gcgatgcaaa gcgtgggtac tatcttgatt
    36901 gttgccttat tgattacgcc agcagcgaca gcttatttat atgctaatag cttaaaagta
    36961 atgttagtga tgtcatcttt actaggcgct ttagcatcgg ttttagggct ttatttgggc
    37021 tataccttta atgttgccgc agggtcaagt atcgtactga cttctgcgat gatgtttttg
    37081 atcagtttct ttgtttcacc caagcaaggc tacctcaaaa gatggatgca aaaaaaagaa
    37141 aaaacacctt aaacttttcg ctttaaacac cttagtggtt tgcaaatagt agcaaataac
    37201 atcaaagggt gttttttata tgtcatcaaa agaccataaa aaaccttatc aaaccaaggt
    37261 atttagccta ttatctgata aggttttttt aagcatgatt tagtttttaa agcgataatc
    37321 tttgacaatg tcaattgagg taatcgtaat gtcttgttct ggtttatcat tttggttgat
    37381 agaagtcgcg gcaattttat cgacaacatc cataccatca atgacttgac caaatacagt
    37441 ataaccgcca tctaagcttg gattgccgcc atgctcatag gcagagatga taggttttgg
    37501 gtagttggta cttgataatc ccttgctttg atttttcttg ttttggttga tataaaattg
    37561 gctaccgttc gtattagcgc cagcatttgc catggcaaga gcaccgcgaa tatgatataa
    37621 aaatggagag atttcgttga caaagccatt gccagcatct tttttaggat ccttgccctt
    37681 ccaaatcgat tcaccacctg tgccatctcc tttggggtca cctgattgaa tcataaagtc
    37741 gttgatcaca cgatggaaag taaggttatc atagtagcct tttttagcat gggtgaggaa
    37801 gttctcaaca gctaagggag catattttgg gaataactta agggtaatgt ccccttgact
    37861 tgtcctcatt acgaccttag cttcttcttt gccgacttct ttggttagtt gtggaaattg
    37921 gctagcatca gctttgagag ctttttgaat gctttcttca taggctttag aagctgcttc
    37981 gctttcttct ttggcagttt tttcatcaat gtatttatcg ccttttatag cgcggtcaac
    38041 agactcacaa gcactcaaac taagtaaact aatggctact aaactgagag ataataattt
    38101 tttcatgtct actcctaaga atgattttct ttattttatc ataaaatgca acagaaaata
    38161 gtagtaaaga gttaaaaacg cctatttttg tgttttatga tctaaccttg gttaaaaggc
    38221 tgctacgact gttattatag cacaagatgc tatagaaaaa cttaaaacac cttggttttt
    38281 tagagctatt tcctaatagt taaaaaatgg tataatagtg ttatggttaa aagaaatcaa
    38341 agaagaaaaa gtgcgcctaa gaagcgtctg acaaaagcag aagtggaaaa gcaacgtgcc
    38401 attaagagga tgattttatc tgttttaatg gctttgttgc ttatttttgc gatgcttcgc
    38461 ttgggcgtct ttggcgtgac aacttataat atgattcgtt ttttggtggg tagtttagcc
    38521 tatccgttta tgtttgcctg gttaatttac ttattttgtt ttaaatggtt acggcaaaaa
    38581 gatggaatga ttgcgggagt tgttattgcc tttttgggat tgctagttga gtggcacgcc
    38641 ttcttatttg cgatgcctag gatgtttgat caagatattt ttctaagcac tgctcggcta
    38701 attacaagag atttactagc cctgcgcgtg actgaatttg ttggtggagg aatgcttggt
    38761 gcattacttt ataaacccat agccttttta ttttcgaata tcggctctta ctttattggt
    38821 tttttgttta tcttattagg attatttcta atgacgcctt gggatatcta tgatgtgagt
    38881 cactttgtga aagaggctgt tgataaacta gcagtggctt atcaggaaaa taaagaaaag
    38941 cggttcatca aacgagaaga gcaccgctta caagctgaaa aagaggcttt agagaaacaa
    39001 gcacaagagg aagaaaaacg tctagcagaa ttgacggttg atcctgagac gggagagatt
    39061 gttgaggata gtcagtctca agtcagctat gatttggctg aggatatgac aaaagaacca
    39121 gagattcttg cttatgatag tcacctcaaa gacgatgagg caagcttatt tgaccaagaa
    39181 gacttggctt acgctcacga gaaaattggg gcttatgact ctttatctgc tttagcatct
    39241 tctgaagatg agatggatat ggatgaacct gttgaagttg atttcacacc aaaaacccat
    39301 cttctttata aacttccaac gatagattta tttgcaccag ataagcccaa aaaccagtcc
    39361 aaagagaaaa acttagtccg caaaaatatc aaggtgcttg aagatacctt ccaaagtttt
    39421 gggattgatg tcaaggtaga acgagcagaa atcgggcctt ccgtcaccaa atatgaaatc
    39481 aagccagctg tcggggtccg agttaatcgt atttcaaatc ttgctgatga tttggcactt
    39541 gctctggctg ctaaagatgt tcgtatagaa gcaccaattc ctggaaaatc cttaataggt
    39601 attgaagttc ctaactcaga aattgcaacg gtttctttcc gcgaactttg ggaacaatct
    39661 gatgccaatc ctgaaaacct tttagaagta ccactaggta aagctgttaa tggtaacgct
    39721 cgcagtttta acttagctag aatgccgcat cttttggtag ctggttcaac tggttcaggt
    39781 aaatcggttg ctgttaatgg cattatttca agtattctga tgaaggctag accagaccaa
    39841 gtgaaatttt tgatggtcga ccctaagatg gttgagttgt ctgtttataa tgatattccc
    39901 catttgctga ttccagtagt gaccaatcct cgtaaagcca gcaaggcgtt gcaaaaagtg
    39961 gttgatgaga tggaaaatcg ctatgaactc tttagtaaag tcggggttcg aaatattgct
    40021 ggttacaatg caaaagttga ggattacaat aggcaatctg agcaaaaaca aatgccttta
    40081 ccattgattg tggttattgt ggatgagtta gctgatttga tgatggtggc tagtaaagaa
    40141 gtggaagacg ctattatccg tctggggcaa aaggcgcgtg cggcaggtat tcacatgatt
    40201 cttgcaaccc aaagaccatc tgtggatgtt atttctggac tgattaaggc caatgtgcca
    40261 tcccgtattg catttgccgt atcttcagga acagatagtc gaacgattct ggatgaaaat
    40321 ggcgctgaaa agctcttagg acgtggagat atgctcttta agccgattga tgaaaatcac
    40381 cctgttcgtc tgcaagggtc tttcatctca gatgatgatg tggaacgaat tgtaaacttt
    40441 atcaaagacc aagctgaagc ggattatgat gacgcctttg atcctggtga agttagcgac
    40501 aatgatcctg gtttttctgg aaatggtgga gctgctgaag gtgatcctct ttttgaagaa
    40561 gcaaaagcct tggtcttaga aacacaaaaa gctagtgcct caatgattca gaggcgtttg
    40621 tcggttggat ttaatcgtgc cacgcgcctg atggatgaat tggaagaagc aggtgttatc
    40681 ggtccagcag aaggaaccaa acctcgtaag gtattgcaga caaactaaaa gaacctgacg
    40741 tctcatcatg agatgtcagg ttcttttttg gtgatgtcag gccagttgat gagatgccta
    40801 tgctaatgta aaaataaaga taactagaat aaggtagaaa ataacggtga ctaaaaagcc
    40861 aactcgccaa aatagcttca taaagcgatg gtaagaaaac gactgagttc ggatgactag
    40921 cagtagtgaa atgacgatgg ctagcagcga catgataata aggtaataag gcagtaaatt
    40981 atgggtgaaa aattttcctg agacaagcac aatttctatc gcaaaaagta aaagtgcgac
    41041 atctggaaat ttaaatccct ttttattaag tttaaataga ggggttacaa tcatggcaaa
    41101 aagaggcgtt acaatcaaga aagctaaggc agttaattta tagatcatca taggtctatt
    41161 ttatactcaa atcccttact tgtaaactga tacttacctg ttgtgataga aactgatctt
    41221 tgagagggaa agagcctact tcaatttaaa cattacaaaa ggcatcagct attttaacaa
    41281 aaagacttga tttattttgg caataagcta taatggtaag gtatgcaata ttgcatatct
    41341 gtgggaggta aaaatctcaa ttaccgccaa aaccacaaca ggaggatttt taaaatggct
    41401 aaaaaagtcg aaaaacttgt aaaacttcaa atccctgctg gtaaagctac accagctcca
    41461 ccagttggac cagctcttgg tcaagcaggt atcaacatca tgggcttcac taaagaattt
    41521 aacgctcgta cagctgatca agctggtatg atcatcccag ttgttatctc agtttatgaa
    41581 gacaaatcat ttgatttcat cactaaaaca ccaccagctg ctgttctttt gaaaaaagct
    41641 gcaggtgttg aaaaaggatc aggtacacct aacactacta aggttgcgac agttactcgt
    41701 gcacaagtac aagaaattgc tgaaactaag atgccagatt tgaacgctgc aaacattgaa
    41761 gctgcaatgc gtatgatcga aggtactgct cgttctatgg gattcactgt tactgactag
    41821 tcagtcaaga acccctatta cccgcacgac ttcatcataa gagatgagtg acgtgggaga
    41881 ttttaaatca aatcgatatg accacattac aaggagaatt ttaaaatggc taaaaaaagc
    41941 aaacaaatgc gtgctgcact tgaaaaagta gatagcacaa aagcgtacag tgtagaagaa
    42001 gctgtagcat tagtaaaaga aacgaacttc gcaaaatttg atgcgtctgt tgaagttgct
    42061 tacaacttga acattgacgt tcgtaaagca gaccaacaaa tccgtggcgc aatggtattg
    42121 ccaaacggaa ctggtaaaac acaacgtgtt cttgtttttg cacgtggtgc aaaagctgaa
    42181 gaagcaaaag cagctggtgc agacttcgta ggtgaagatg accttgttgc aaaaatcaat
    42241 ggtggatggc ttgactttga cgttgttatc gcaacgccag acatgatggc tatcgtaggt
    42301 cgccttggac gtgtccttgg acctcgtaac ttgatgccaa accctaaaac tggtacagta
    42361 acgatggatg ttgctaaagc cgttgaagag tctaaaggtg gtaaaatcac ttaccgtgct
    42421 gacaaagcag gtaatgttca agctcttatt ggtaaagttt catttgatgc tgacaaattg
    42481 gttgaaaact tcaaagcctt ccacgatgta atggctaaag ctaaacctgc aacagctaaa
    42541 ggaacttaca tggcaaacgt ctcaatcaca tcaacacaag gtgttggtat caaggttgat
    42601 cctaactcac tttaagaaaa aaattagggc aacgcctaat catagcaaaa agacaaattt
    42661 ctgaagacaa atcttcacta gaggtttgtc ttttttatgg tttaaaatgg aatgcaaaca
    42721 aaaaagtgta ggcgataata ccatgaaagg agttgattct cttaatgaga tgtggtgtta
    42781 acacatcatg agctcacaaa aaatgataaa ggaagtatcg gtggtgttaa aaaagggatt
    42841 ttaattgaca tttattagcc tctaaaatgt cctttcccta ttcataattg agggtttgtg
    42901 gtaaaatagt aaggacaaac taaggagaac atagtagtgg aacctaaata tcaacgtata
    42961 ttaattaagt tatctggtga agcattagca ggtgaaaaag gagttggcat tgacattcca
    43021 actgttcagg ctattgcaaa agaaattgcc gaagttcatg tgtcgggagt acagatcgct
    43081 cttgttattg gtggaggcaa tctctggcgt ggagagcctg cagcagacgc tggaatggac
    43141 cgtgttcagg ctgattacac tggcatgctt ggaacggtga tgaatgccct agtcatggct
    43201 gatagtcttc aacattatgg tgttgatacc cgtgtgcaaa cagctattcc tatgcaaaat
    43261 gtagcagaac cttatattcg cggacgtgcc ttgcgccatc tagaaaaaaa tcggattgtt
    43321 gtttttggag ctggtattgg ctcgccttat ttttcaacag acaccaccgc ggcactgcgt
    43381 gctgctgaga ttgaagcaga tgctatttta atggcaaaaa atggtgtgga tggggtctat
    43441 aatgctgatc ctaaaaaaga tgctaatgcg gttaaatttg atgaattaac acatggtgaa
    43501 gtgatcaaac gtggtctcaa aatcatggat gcaacggcat cgaccttatc gatggataac
    43561 gatattgatt tggtggtttt caatatgaat gaagctggca atattcaacg tgttgtcttt
    43621 ggagaacata ttggaactac tgtatcaaat aaagtttgtg actaaaagtt agaaaagaaa
    43681 gagagtaatt atcatggcaa atgcaattat tgaaactgca aaagaacgtt ttgcacaatc
    43741 ccatcagtct ttatcacgtg aatatgccag cattcgtgcg ggtcgtgcca acgctagtct
    43801 tttagaccgt atccaagttg attattacgg ggcaccgaca ccattaaatc aattggcttc
    43861 aatcactgta ccagaagcgc gtgtcttatt gatctcgcct tttgataaat cttctatcaa
    43921 agatatcgag cgcgctctta acgcatcaga tttaggcatt acacctgcta acgatggctc
    43981 tgtcattcgt ttggttattc cagctttgac cgaagaaaca cgtaaagaat tagccaaaga
    44041 agtgaaaaaa gttggtgaaa atgctaaaat tgcaatccgt aatatccgcc gtgatgctat
    44101 ggatgatgct aaaaagcaag aaaaagcaaa agaaatcact gaagatgagt taaaaacact
    44161 tgagaaagat atccaaaaag caaccgatga tgctatcaaa gaaattgacc gcatgacggc
    44221 tgaaaaagaa aaagaattgc tctcagtgta acaggatttc tacgaaaaat agacgactat
    44281 agacacaaaa agataggtga ctatttcaga agcgtttggt tttgatttgt ccctattttt
    44341 ttaaagaaag aaaaaagata tgaatgattt actagcaaca gtcattactg ggcttattaa
    44401 agaagaaaac gctaatgact attttatcca aaaagagggg tttaccttta ccttgtctaa
    44461 agcagaagga gaacgccaga ttggtgatat ggtcacaggc tttgcctata cagacataaa
    44521 gcaaaaggca cgtctcacaa ccaaagagat ccgttcaaca cggacaagtt atggctgggg
    44581 ggaagtgaca gaggttcgtc gtgacctagg cgtttttgtc gatacaggaa ttccaaataa
    44641 agaaattgtg gtttctttgg acgtgttgcc tgaaatgaaa gaactatggc ctaaaaaagg
    44701 ggataaactt tacattagtt tggatgtgga taaaaaagac cgtatttggg gacttccagc
    44761 agagcctgag gtctttcaaa agatggctag ccctgcatac aataatatgc aaaatcaaca
    44821 ttggccagca attgtttacc gcctaaaatt aacaggaacg tttgtttacc tgcctgaaaa
    44881 caacatgtta ggctttattc acccaagtga acgctacgca gaaccgcgct taggtcaggt
    44941 tttagatgcc cgtgtgattg ggtttagaga ggtcgatcgt accctaaact tgtcgttgaa
    45001 accacgttct tttgagatgt tggagaatga cgctcagatg attgtcacct atcttgaagc
    45061 caatggtggt ttcatgacct taaatgacaa atcaagtcca gaagagatta aagcaacctt
    45121 tggtatttca aaaggccagt ttaaaaaagc cttgggtgga ttgatgaaag ctaaacgcat
    45181 caaacaagac gccacaggaa ctgaattaat aggataaaaa tgctttgcca ctggcaaagt
    45241 tttttgttac aatgaagacc attaagtgaa gaaaaagggg atatagagaa tggaaagagc
    45301 catttttgca ggaggttgtt tctggtgtat ggtgcaacct tttgaggagc aagcaggtat
    45361 tttgtcagtc agaagtggct acacaggtgg gcatctccca aatcctagct atgaacaagt
    45421 ttgtgccaaa acaacaggac atacagaagc tgttgaaatt atttttgacc ctaagcaaat
    45481 cgcttataaa gatttggtag agctctattg ggcgcagacg gatccaacag acgcctttgg
    45541 gcaatttgaa gatcgtgggg acaactaccg tcctgtcatt tactacacaa cggaaagaca
    45601 aaaagaaatt gcagagcaat caaaagcaaa cttgcaagct tcaggacgtt ttgaccaacc
    45661 cattgtcacc actattgaac cagcagagcc attttatctc gcagaggatt atcaccaagg
    45721 attttataaa aagaatccca agcggtatgc acaaagcagt gctattcgtc atcagttttt
    45781 agaggagaac tggtcatgag aaaatcattt tacagctggc tgatgacaca acgtaaccct
    45841 aaatcaaatg aacctgtggc gattttagca gatttggtgt ttgatgacac cacttttcca
    45901 aagcatacca atgattttga actcattagt cggtatctag aagatcaagc tagtttttcc
    45961 tttaatttag gtcaatttga tgaaatctgg gaagattatc tagcacatta atacgtggat
    46021 aaaaaagagt catatcgact cttttgaagg gttatcacgt tattattaca aaatgacatc
    46081 actttctttc aaaaaaatga caagctactt tttttcttaa aaatatgcta tgatatagct
    46141 tagaaaaaaa ataaaaagga agcagagatg attattacta aaaagagctt atttgtgaca
    46201 agtgtcgctt tgtcgttagc acctttggcg acagcgcagg cacaagagtg gacaccacga
    46261 tcagttacag aaatcaagtc tgaactcgtc ctagttgata atgtttttac ttatactgta
    46321 aaatacggtg acactttaag cacaattgct gaagcaatgg gaattgatgt gcatgtctta
    46381 ggagatatta atcatattgc taatattgac ttaatttttc cagacacgat cctaacagca
    46441 aactacaacc aacacggtca ggcaacgact ttgacggttc aagcgcctgc ttctagtcca
    46501 gctagcgtta gtcatgtacc tagcagtgag ccattacccc aagcatctgc cacctctcaa
    46561 tcgactgttc ctatggcacc atctgcgaca ccatctgatg tcccaacgac accattagca
    46621 tctgcaaagc cagatagttt tgtgacagcg tcatctgagc tcacatcatc aacgaatgat
    46681 gtttcgactg agttgtctag cgaatcacaa aagcagccag aagtaccaca agaagcagtt
    46741 ccaactccta aagcagctga aacgactgaa gtcgaaccta agacagacat ctcagaagac
    46801 ccaacttcag ctaataggcc tgtacctaac gagagtgctt cagaagaagt ttcttctgcg
    46861 gccccagcac aagccccagc agaaaaagaa gaaacctctg cgccagcagc acaaaaagct
    46921 gtagctgaca ccacaagtgt tgcaacctca aacggccttt cttacgctcc aaaccatgcc
    46981 tacaatccaa tgaatgcagg gcttcaacca caaacagcag ccttcaaaga agaagtggct
    47041 tctgcctttg gtattacgtc atttagtggt taccgtccag gtgatccagg agatcatggt
    47101 aaaggtttgg ccattgattt tatggtgcct gaaaattctg ctcttggtga tcaagttgct
    47161 caatatgcca ttgaccatat ggcagagcgt ggtatttcat acgttatttg gaaacagcga
    47221 ttctatgcgc catttgcaag tatttacgga ccagcttaca catggaaccc catgccagat
    47281 cgcggcagta ttacagaaaa ccattatgat catgttcatg tctcctttaa tgcttaattc
    47341 atgctatcaa aaaaaactca cgtttaggcg tgagtttttt gacaattctg acagagtaag
    47401 ccctataaga ggctgagata cgcttttaat acctgttgtt tttctcaccc tttagacagt
    47461 tttaacgtta tgtctaaaag agatggtttt tattagcgca aatatgggtt taggggtaaa
    47521 atataggtaa ttaacctgat atgatcatga taaaggtaga agaataccta ttatttcgtg
    47581 acaactgagc aatctgtctt acctttggag tgatgatatc actttgcttg actattcaag
    47641 gaggtcttga aatgtattat actagtggta attacgaagc ttttgcaaca cctcgaaaac
    47701 ctgaaggggt agatcagaaa tcagcttata ttgttggcac tggtttagct ggtttagcag
    47761 cagctgtttt ccttattcgc gatgggcata tggctgggga acgcattcat ctgtttgagg
    47821 aattgccttt agcaggtggt tctttagatg gtattgaaaa gcctcatctt ggttttgtga
    47881 cccgtggtgg tcgtgagatg gaaaatcatt ttgagtgtat gtgggacatg tatcgctcta
    47941 ttccctcact ggaaattccc ggtgcgtctt atttagatga attttattgg ttggataagg
    48001 atgatcctaa ctcatccaac tgtcgtttga ttcacaagag aggaaatcgt gtggatgatg
    48061 acggccagta tacgctcggt aaacagtcaa aagaattaat ccatttaatc atgaagacag
    48121 aagaatctct aggagaccaa accattgaag agttcttctc agaagatttc tttaagagta
    48181 atttttgggt gtattgggca accatgtttg cttttgaaaa atggcactct gctgtagaaa
    48241 tgcggcgcta tgcgatgagg tttatccacc atattgatgg tttgccagat tttacctccc
    48301 tcaagttcaa caaatataac caatatgact ctatggtcaa accgattatt gcttacctag
    48361 aatcacacga cgttgacatc caatttgaca caaaagtcac tgatattcag gtggaacaaa
    48421 cagctggtaa aaaggtagca aaaaccatcc atatgacggt gtctggggag gctaaggcga
    48481 ttgagctaac acctgatgat ttggtttttg tgaccaatgg ttctattact gaaagcagca
    48541 catacggtag tcatcacgaa gtggctaagc caaccaaagc gttaggtggt tcttggaatt
    48601 tatgggaaaa tctagctgct caatcagatg attttggtca tcctaaagtg ttttaccagg
    48661 acttgcctgc tgaaagctgg tttgtgtctg ccacagcaac cataaaacac ccagctatcg
    48721 agccttatat tgaacgtttg acccaccgtg acttgcacga tggcaaagtg aacactggcg
    48781 gcatcatcac tattacagat tctaactgga tgatgagctt tgccattcac cgtcaacctc
    48841 attttaaaga acaaaaagaa aatgagacca ctgtctggat ttacggtctt tattccaata
    48901 gtaagggcaa ttacgtccac aagaaaattg aggagtgtac aggtcaagaa atcacagaag
    48961 aatggttgta ccaccttggg gtacctgttg ataaaatcaa ggacttagcg agtcaggact
    49021 atatcaatac agttcctgtt tacatgcctt atattacgag ttactttatg ccacgcgtca
    49081 aaggagaccg tccgaaagtt atcccagatg gttcagtcaa cttggccttt attggtaact
    49141 ttgcggaatc tccatctcga gatacggtct ttacgactga gtattctatt cgtactgcca
    49201 tggaagcagt gtatagcttc ttgaatgtgg aacgaggcat cccagaagtc tttaattcag
    49261 cctatgatat tcgtgaattg ctcaaagcct tttattacct taatgataaa aaggcaatca
    49321 aggatatgga tttgccaatt cctgcactga ttgagaaaat cggacataaa aaaatcaagg
    49381 atacctttat cgaagaattg ctcaaagatg ctaatcttat ttaacatgcc ttagagtagt
    49441 taaagctgat aagtttaggc ttatcagctt ttttgatgat ttatagaaaa gtgataaagg
    49501 aaaagtctga cgtgaacgtc acataagaat ttaaaatttg ttataatgta agcaataaag
    49561 atgaaagaaa gagggactct atttgcaaga gtattctatt gacattacct taacccatcc
    49621 tgatgatgtt ttagctcttt ttggtagcaa cgagcgtcat ttgaagctaa tagaagctca
    49681 tttaggagtt attgttcacg caagaaccga gcgagtccaa gtgattggtg atgatgaaga
    49741 agctgtggaa ttggctcgat tgaccatcaa agccctactg gttctagtgg gtcgtggcat
    49801 ggtggtcaac acatcagatg tggtgacagc tttatctatg gcagaatctc atcagattga
    49861 ccaatttatg gcgctttatg aagaagaaat cattaaagat aactatggta aggcaatacg
    49921 agtcaaaaca ttaggtcaaa aaacctatgt cgatagtgtc aaacgtcatg atgtggtttt
    49981 tggtgtagga cctgcaggga cagggaaaac cttcttagct gttacacttg cagtcactgc
    50041 tctgaaaaga ggacaggtaa agcgtattat tttgacgcgt cctgcggtag aagctggtga
    50101 aagcctaggt tttttaccag gcgatttgaa agaaaaggtg gatccttacc tcagaccggt
    50161 ttacgatgcc ctttatcaca ttttaggcaa ggaacaaacc acacgcctga tggaacgaga
    50221 tgtgattgag attgctccgt tagcttatat gcggggtcgt actctggatg acgcttttgt
    50281 tatcttagat gaggctcaaa acacaaccat catgcaaatg aagatgtttt tgacccgtct
    50341 tggttttaat tctaaaatga ttgtcaatgg agatacgagt cagattgacc tccctcgaaa
    50401 tgtcaagtct ggtttgattg atgctactca gaaattacaa ggcattaaac agattgattt
    50461 tgtctatttc tctgctaagg atgttgtccg acatcctgtt gtggctgata ttatcaaggc
    50521 ttatgaaacg tcctcagagg agacgaagga cttactaaaa cctaaggcta gtgacgttga
    50581 agctgattac cagccaggct tgacaaaata ccctgtgatc ggtcaagagc actaagactt
    50641 ttacgatgat gtttaatatg gcctttttaa ggaggattct atggatgatt taaccaaagc
    50701 tattatggca gatgaggcta atctctccta taccgaaaga ggtatttttc ctttatatga
    50761 tgctcctcaa acagctagga ttattattgt tggccaagct cctggaatag tagctcaaga
    50821 aaccaaactt tactggaatg atcgtagtgg catccgctta cgagactggc taggtgtgga
    50881 caatgacacc ttttaccact caggattgtt tggcattatc cctatggatt tctattatcc
    50941 tggtaaggga aaatccggtg acttaccacc tagagaaggt tttgcggcta agtggcaccc
    51001 gccattaaga gcattgatgc cagaagttga attgacgatt ttggttggtc gctatgctca
    51061 ggacttttac cttggtaaca aggcttacaa aaccttaact gaaacagtcc gtcactttga
    51121 ggagtacttg ccagactatt ttccacttgt tcatccttct ccaagaaacc aattgtggtt
    51181 agctaagaac ccctggtttg agcaggattt attacctatc ttgcaaaaac gtgtggaggc
    51241 aattctaacc aaataggccc tgatttacct agcattccca tttttaaggg gcttgtggta
    51301 taatgggtaa ataacaaatg ctgattgaaa ggataaggga aattatctgt cagataataa
    51361 tgggtgccca acataccaaa tgaggtgggc agcccttccc taacaattaa ccttatgtat
    51421 atcgagatga ttgacgaaac gggacaagtt tcgcaagaga ttatggagca aacacttgat
    51481 ttgctcaatt ttgctgctca aaagacgggc aaagaagaaa aagaaatgtc tgtgaccttt
    51541 gtgaccaatg aaagaagtca tgaactcaac ttagaatacc gagatactga tcgtccaaca
    51601 gatgtgattt ctctagaata taagccagaa acgcctattt tatttagtca agaggatttg
    51661 gctgctgatc ctagtttggc agagatgatg gcagagtttg atgcttatat tggcgaattg
    51721 tttatttcta ttgacaaggc gcgtgagcag tcccaagaat atgggcactc ttttgagcgt
    51781 gaaatgggat ttttagctgt tcatggattc ttacatatta atgggtatga tcactacacc
    51841 cttgaagaag aaaaagagat gtttacctta caggaagaga ttttgactgc ttatggcctt
    51901 acacgacaat aataccacca aacggaaatg gaaaaacaga accattacct caagcttgga
    51961 gtttgccttg acaggggttt tcacagcttt taaggaagaa cgcaatctca gaagccacct
    52021 tttgtctgcc tgtttagctt gtgttgctgg tttatttttt agcatttcag ccatcgaatg
    52081 gttattttta cttctagcca tttttttggt cattacctta gaaattgtca attctgctat
    52141 tgaaaatgta gttgatttgg ctagtgacta tcacttttct atgctagcta aaaatgccaa
    52201 ggatatggca gcaggtgctg ttttgatgat ttctggctat gctgtcttga caggacttat
    52261 tatttttatt cctaaaattt ggaatatttt cgttcattaa aaagaaagga atgggctatc
    52321 ctatgtgatg atggcatagg agaataagga aaggacaaga cggttaagct tagtgacaag
    52381 cttgatctga agccctaccg ttaaggcccg taattatcta tgtttaaatc aggctttgta
    52441 gccattcttg gtcgtccaaa tgttggcaaa tccacctttt taaatcacgt catggggcaa
    52501 aaaattgcca tcatgagtga caaggctcag accacccgta ataaaattat gggcatttac
    52561 accacagaaa cagaacaaat tgtctttatt gatacgccag gtattcataa accaaagacc
    52621 gcccttggtg attttatggt tgaatctgct tatagtactt tacgtgaagt tgagacagtt
    52681 cttttcatgg ttcctgccga tgaaaagcgt ggtaagggtg acgacatgat tatcgagcgg
    52741 ctaaaggcag ctaagattcc tgttatttta gtcatcaaca aaattgataa ggttcatcca
    52801 gaccaacttt tggaacagat tgatgacttc cgtagccaaa tggattttaa agaagtggtc
    52861 ccaatttctg ccctagaagg taataatgtg ccaaccctaa tcaagttatt aactgataat
    52921 cttgaagaag gtttccaata ttttccagaa gatcagatta ctgatcatcc agaacgtttc
    52981 cttgtgtcag aaatggttcg tgagaaggta ctgcatttga cgcaacaaga ggttccacat
    53041 tccgtagctg ttgttgtcga atccatgaag cgagatgaag agactgataa agtgcatatc
    53101 cgtgcaacca ttatggtgga gcgcgatagc caaaaaggca tcatcatcgg taaacaagga
    53161 gccatgctca aaaaaatcgg aaaaatggcg cgccgtgata ttgaactcat gttaggtgac
    53221 aaggtctact tagaaacgtg ggtcaaagtc aagaaaaact ggcgggataa aaagcttgat
    53281 ttagctgatt ttggctataa tgagaaagaa tactaacata gtaaagcgag atcatatgcc
    53341 acaagactac atttcttata tccgctccaa agttgggcat gataagatta ttcttaactt
    53401 tgctggtggg attttgacca atgatgacgg caaggtgctg atgcagttgc gtggtgataa
    53461 gaaaacctgg gctattcctg gtggcactat ggaactgggt gagagctctt tagagacttg
    53521 caaacgtgag ttcttagagg aaaccggaat tgaggttgaa gctgttcgct tactgaatgt
    53581 ttacactcat tttgaggaag tttatcccaa cggtgatgct gttcagacta ttgtctttat
    53641 ctatgaacta acggctgttt ctgatatggc tattgataac tttcataatg aggaaacgct
    53701 caagttacag tttttctctc atgaggagat agcagagtta gagagtgtct cagccaaaca
    53761 tcgcctgatg ttagaggaat attttagtga tagctttgct atggggcatt aagaactctc
    53821 taggacagtt ggagtatttt tggagattgg cttgttaaaa aaatttttga aagacgatgt
    53881 ttcgagagct tatgctctct ttttttgctt ccaatttttc tgattaagtc tgaataattt
    53941 tgttaaatgt taacaggatt ttctttccaa ttgatactag cagacatcaa acatttgttt
    54001 tagagttatg acgaattgcc acctagccat gcatgactta ggcaagttga acctaactgt
    54061 ggcaatagac ttggaagggg tgacaagaat catttcctaa aagaattgat gaagtgccta
    54121 tccattattc gaattgtaaa gttcgtctaa gtaaagttga atttgatctc gaatggactt
    54181 tgatgattta tgacttgttg tgtcaataac accaaaaact cgataatgat ttttaaatat
    54241 ttcctcgtaa ttaatatgat ttccgattag aataatatga gcatgaggat aacgcatacg
    54301 tatttcgttt agaaggtcat atacttgctc agatttaaaa aagtatg
//
LOCUS       DAUGHY010000005        54347 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.005, whole genome shotgun sequence.
ACCESSION   DAUGHY010000005 DAUGHY010000000
VERSION     DAUGHY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54347
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.005"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            34..107
                     /locus_tag="VH752_000285"
     tRNA            34..107
                     /locus_tag="VH752_000285"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68..70,aa:Ile,seq:gat)
     gene            130..201
                     /locus_tag="VH752_000286"
     tRNA            130..201
                     /locus_tag="VH752_000286"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:163..165,aa:Glu,seq:ttc)
     gene            complement(495..896)
                     /locus_tag="VH752_000287"
                     /pseudo
     CDS             complement(495..896)
                     /locus_tag="VH752_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1319..1852)
                     /locus_tag="VH752_000288"
     CDS             complement(1319..1852)
                     /locus_tag="VH752_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEQ8288667.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
     gene            complement(1932..2708)
                     /gene="recX"
                     /locus_tag="VH752_000289"
     CDS             complement(1932..2708)
                     /gene="recX"
                     /locus_tag="VH752_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEQ8288668.1"
                     /translation="MKITKIEKKKRLYLIELDNDESLYVTEDTIVRFMLSKDKVLDND
                     QLEDMKHFAQLSYGKNLALYFLSFQQRSNKQVADYLRKHEIEEHIIPDIITQLQEEQW
                     IDDTKLADTYIRQNQLNGDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQK
                     LFDKYQEKLPPKALKDKITQALLTKGFSYDLAKHSLNHLNFDQDNQEIENLLDKELDK
                     QYRKLSRKYDGYTLKQKLYQALYRKGYNSDDINCKLRNYL"
     gene            2823..4178
                     /gene="rlmD"
                     /locus_tag="VH752_000290"
     CDS             2823..4178
                     /gene="rlmD"
                     /locus_tag="VH752_000290"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ8288669.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIFC
                     QITSVKRNFAEAKLLMVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLARFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVVKALDVGSKDHIIDAYCGVGSIGFAFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIIPKWYKQGYRADAIIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKVYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
     gene            4842..6197
                     /gene="rocA"
                     /locus_tag="VH752_000291"
     CDS             4842..6197
                     /gene="rocA"
                     /locus_tag="VH752_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HEQ8288670.1"
                     /translation="MLEDFLQFLGFIFLDIIEIMLTLKLFSFVSAIPLRLKNIFYLSL
                     SMVLFQVVFWAFFPDHFILDVVMLAQFLFFALIALYYGKSIKAKFLMFYAFFPLVSIS
                     LVKRFIVFFVMPLFGMPYSVVKHNTLLIYSITCFSIFLIYRCIQVFHFDFSTWRQYFQ
                     SHRASKLLVFTNSSMALYYLCVQGIDVMSPSLSGLATTTARSIIVLFYFILFLTLLIH
                     LERYVKQNSIEAIVQQKEYRELINYSQHLGLLYQDIQELRQLLTTVSSRLKIGIEQND
                     ISIVRLTYEGILNAEKNNAKDDRLDLTCLDKLQVEAIRHIVLAKLIEAKNKKLKVEVS
                     IPNCIATFFLEVVDFTKLLSFLLDNAIEMSLETKQPCLSIAFLDQNHKLVIVIQSSTK
                     QGQDDSQSVFAIPALKKRDDWQFDLRNVTTILNRYDYLTISSQIHDGILTQLIEIAKP
                     D"
     gene            complement(6298..9003)
                     /locus_tag="VH752_000292"
     CDS             complement(6298..9003)
                     /locus_tag="VH752_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEQ8288671.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTNP
                     NFHSFHLDGQTIILDDYLKVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NMLIGKEDCDKWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTSEKYSSQFSEISWQGPDDSRILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLADLADDLPENL
                     STVQGEITSQETDGWYTLANTASARIYLKQANTRVSRQLENITEPLAAMAYEVTSTYP
                     HDQLRYAWKTLMQNHPHDSICGCSVDSVHREMMTRFEKAYEVGHYLAKEAAKQIADAI
                     DTRDFPMDSQPFVLFNTSGHSKTSVAELSLTWKTYHFGQRFPKEVYQEAQEYLAGLSQ
                     SFQIIDTSGQVSPEAEILGTSIAFDYDLPERSFREPYFAIKVRLRLPITLPAMSWKTL
                     ALKLGNETTPSETDSLYDDSNQCLENGFLKVMIQTDGRLTITDKQSGLIYQDLLRFED
                     CGDIGNEYISRQPTHDQPFYADQGVTHISVRSNTIQVAELEIQQTFTIPVSADDRLQA
                     EMEAVIDITERQARRSQEKAELTLTTLIRMEKNNPRLQFTTRFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPDATFWKNPSNPQHQECFVSLFDGENGVTIGNYGLNE
                     YEILPDTNTIAITLLRSVGEMGDWGYFPTPEAQCLGKHSLSYSFESITKQTQFASYWR
                     AQEGQVPVITTQTNQHEGTLAAEYSYLTGTNDQVALTAFKRRLADNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(9018..10304)
                     /locus_tag="VH752_000293"
     CDS             complement(9018..10304)
                     /locus_tag="VH752_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEQ8288672.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLLTGDIPAMWLRDSTAQIKPYLFLAKEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANEKGHQTDHTQMNPWIWERKYEIDSLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHAGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVVLSYLEDLYNNLFHNEPIATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCAKDDPIYLATRRTIFSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALALQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSIAP"
     gene            10437..11477
                     /locus_tag="VH752_000294"
     CDS             10437..11477
                     /locus_tag="VH752_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8288673.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAKKQVTSPEANKDLLLILPFASDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPAVVADNKGGAYQLTQHLIEQGCDQIWFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LSIPDQVAIVGFDNSQASRLLSPKLSTAAQDLYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            11528..13222
                     /locus_tag="VH752_000295"
     CDS             11528..13222
                     /locus_tag="VH752_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HEQ8288674.1"
                     /translation="MTIYHALKDYQEVMTRGDYLVFDTPLTCRFIGHFFRFENQKALL
                     AELATSKYFQWIEEGQAEVTMKHFFNRQLAKDAFTLKVSEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFPMRGIIEGFYGTPWTREERIDCLRFIGNKRM
                     NTYMYAPKDDDYQRKLWRDLYPEDWVTYFKELLAVAKEEGIDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEVAFFWTGPSTLASQISQADIETM
                     AAVYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQPDYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSHSWLETLTDYTEDTELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKDVSTLSAWVAELVECVNTLRKLDKPAFQQAIAPWFERVAKDQVFWQAM
                     LNQEPQLETLYADLQEDKHRIGSDIPSRYYRIYYQQQDKLTANQGQVTQARPEDYA"
     gene            13247..14689
                     /locus_tag="VH752_000296"
     CDS             13247..14689
                     /locus_tag="VH752_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ8288675.1"
                     /translation="MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYD
                     YVGPDTASDAYHQIESDLTLLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDLFVAFSKVCFQEFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRQGPAELSDGR
                     IGTILNLTPAYPASQSEADMAAAHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPEWYYDPYLMPGRRMN
                     VDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEERYRDETGQIQDDYRIQF
                     LKEHLTYLHKGIETGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNRLISLEVMEEFGGSASHLSD"
     gene            complement(15300..16178)
                     /locus_tag="VH752_000297"
     CDS             complement(15300..16178)
                     /locus_tag="VH752_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ8288676.1"
                     /translation="MSLLCIDIGGTSLKFALCHNGQLSQQSSFPTPSSLEKFYQLLDQ
                     EVARYSAYHFSGIAVSAPGAVNKEKGIIEGASAIPYIHHFKIQEVLEERLHYPISIEN
                     DANCAALAEATLGAGKGASSLAMLVLGTGVGGSLVIDGKIYHGAHLFGGEFGFMIMND
                     RYQTFSQLGTVVNMAKRYSAIVNNGKDYTGKAVLALAEQGDHLAIKERQVFLQSLAIG
                     IFNIQHAFDPQLILIGGGVSQADFLLSAIEAELDKLYQAVEISDLRPQLAICHFKNEA
                     NLLGAAIDFYQEHKEA"
     gene            16374..17324
                     /locus_tag="VH752_000298"
     CDS             16374..17324
                     /locus_tag="VH752_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ8288677.1"
                     /translation="MTRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIAFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLTGSG
                     ADPIKWYSNPTWWPLILLFMSVWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDAALF"
     gene            17334..18260
                     /locus_tag="VH752_000299"
     CDS             17334..18260
                     /locus_tag="VH752_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ8288678.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            18341..19786
                     /locus_tag="VH752_000300"
     CDS             18341..19786
                     /locus_tag="VH752_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ8288679.1"
                     /translation="MKKWQKIVCVTGTVLAASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKNQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIGDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            20092..20697
                     /locus_tag="VH752_000301"
     CDS             20092..20697
                     /locus_tag="VH752_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEQ8288680.1"
                     /translation="MNGIEKTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTL
                     FLKTSQEENHYSLKSAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFT
                     VSFLIVIAMVWLTMTYLYMVFYAVSYEISLWNNMKLAFISVFLSFKSFLLMLSVLLGI
                     ILVTWQYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            20687..22411
                     /locus_tag="VH752_000302"
     CDS             20687..22411
                     /locus_tag="VH752_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8288681.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLAAIGLSS
                     YSISSTYKRVDAEAQMRLEETLGRLQSQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADRVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVHGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESIADLAIPKFVIQPLVENYFVHGIDYSRNDNALSIKALDETDHLLIQVLDN
                     GRGISQERLADMEKRLQEHQTTGNSSIGLQNVYLRLFHHFRDRVSWSMAKEPNGGFII
                     QIRIRKDA"
     gene            22411..23895
                     /locus_tag="VH752_000303"
     CDS             22411..23895
                     /locus_tag="VH752_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8288682.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDCLDAQQAESIRQEAYHDSLLTLWLTDELSEKEFQQLSQG
                     LPAAALIGFTVLYLDCQEWQAAIDAYFEQEGQPFYLKKEEDKKVYLAVLLGKTDQAKA
                     FTYDLQKRFSRNMHQLILGETVDDWENVYESYNQVLKSLFYNCEVSPIRAKDKSKIEL
                     PESRLQFFAFNKALMIGDEPTILSKLEAIFDEMKTLNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHDSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            23825..27331
                     /locus_tag="VH752_000304"
     CDS             23825..27331
                     /locus_tag="VH752_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEQ8288683.1"
                     /translation="MVYRQKSSEIVTRTITKPSRATSNIKQEIDMTPDSKEQTVTGYQ
                     YHYIDQEGRKQPFNQGWRFLMADVACAQAPSFDDSNWQVIHLPHDFSLTQPYTRNGEA
                     ESAYKLGGVGWYRHYLVLDEVLAGCHVAITFEGSYMETEIYVNGQFIGKHLNGYQEFT
                     YDISDVVTFGAENLLAVRVENKVPSSRWYSGSGLYREVSLSVLPQLHFVADQVAMTLA
                     DTAVQEKGQQKVDLRFALNQPIQTCHYQLSLCLWEQSHCSKDKKLLYQETEVPLADLA
                     FQRQYGLTLSLEELQLWSPDNPHLYDLELTLYYQGQVIDCFCLETGFRQLTFTANQGL
                     FVNGRAVKLKGVCLHHDQGGLGACAYEDALARQLVLLKDMGANTIRSTHNPSSPKLRQ
                     LANRLGFFVIEEAFDTWTYAKNGNVNDFSNYFHQTIGTENANYLQRVRSPETSWAQYS
                     IEAMVWSAKNDPSVLMWSIGNELMEGFSADVSHYPELTRQMCQWIAAIDTSRPITFGD
                     NKLKEADFCWHEEVSQMATLLSQLDHPQGLIGLNYANGKDYDKLHEEHSDWLLYGSET
                     VSAITSRAYYKETKKVLDSGYHLTSYDHAKVDWGAFASQAWYDTITRDFVAGECVWTG
                     FDYLGEPTPWNKIDSGVVGLWPSPKNAYFGILDTAGFPKDSYYFYQSQWVQGQTTLHL
                     LPVWQKDQLCFDEQGLVEVVVYSNAASVQLMFEDEQGNLTDYGRKAFHTYSTPTGHTY
                     QLYQGADAAKNPHENLYLTWRVPYQKGLLRAVAYDISGKSIPKTSGRSQVRTYGSVAK
                     LSWKAFEAPIDAPWELLYLDLSLLDSQGELVSHAQDWLKVQVEGPARLLALDNGNPTD
                     HTPYQEPLRQAYGGKLLAILALTGEAGHIKVTAKASQQLSSVFQTQVACRAPKEHLVT
                     LPLEKYQFKKSRLTSQQEIGQSCLKTTQFLSVLENKDTQQKAGKASTPHILITSYQHY
                     LSLPLQVCSQAFDFKKGDDIFLPQWLQVKDAQGSFVKKAFPMTWCLKEKIFAKPILVT
                     GYVSCFGQSIPVEARLNLAQASKRRHQDLSLVAKQSLATSQKRLTYCYQYDTVQAIGA
                     ISLQNHTGTSLSVSVAISYGSQSSIKQETLMLADSHLSQLEEVIFFDDILTVLYLAVI
                     LEVIEGPDLTEDSIQIGLWDLA"
     gene            27469..28347
                     /locus_tag="VH752_000305"
     CDS             27469..28347
                     /locus_tag="VH752_000305"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ8288684.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEVRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            28582..29337
                     /locus_tag="VH752_000306"
     CDS             28582..29337
                     /locus_tag="VH752_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEQ8288685.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVAVEGLTRHITVSQANALSLPF
                     PDNHFDIVINEAMLTMLNAQAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLDEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            29390..29728
                     /locus_tag="VH752_000307"
     CDS             29390..29728
                     /locus_tag="VH752_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ8288686.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAGIPHALYAVEAFQ
                     MLLVVVKPEV"
     gene            29730..30035
                     /locus_tag="VH752_000308"
     CDS             29730..30035
                     /locus_tag="VH752_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ8288687.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPKDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKVHHLEAKTDCKLLQVLLD"
     gene            complement(30471..31544)
                     /locus_tag="VH752_000309"
     CDS             complement(30471..31544)
                     /locus_tag="VH752_000309"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEQ8288688.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQTKSLERVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKSLTNMIGSIYPPE
                     AIIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKQIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(31600..32352)
                     /locus_tag="VH752_000310"
     CDS             complement(31600..32352)
                     /locus_tag="VH752_000310"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEQ8288689.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKTLSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            complement(32592..32662)
                     /locus_tag="VH752_000311"
     tRNA            complement(32592..32662)
                     /locus_tag="VH752_000311"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32628..32630),aa:Thr,seq:ggt)
     gene            32829..33083
                     /locus_tag="VH752_000312"
     CDS             32829..33083
                     /locus_tag="VH752_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEQ8288690.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            33104..33499
                     /locus_tag="VH752_000313"
     CDS             33104..33499
                     /locus_tag="VH752_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEQ8288691.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVESVETAVDKTIS
                     AGKNLVEKGSQTVENLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASFKK"
     gene            33784..34103
                     /locus_tag="VH752_000314"
                     /pseudo
     CDS             33784..34103
                     /locus_tag="VH752_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            34100..34663
                     /locus_tag="VH752_000315"
     CDS             34100..34663
                     /locus_tag="VH752_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ8288692.1"
                     /translation="MNLIIIGAQASGKMTIGQEVARQTGMTLFHNHDSIDFVLRFMPW
                     SQESTALIERIRFAFFETFAKTGQDMIFTIVIDFNDPNDVAMLEKIQAVFQSYDQEVL
                     FVELKTDIEERLKRNRTENRLKHKPLKRNIEWSEQDIQSTMAYAVFNPEEPPKTLTHY
                     QKINNTQLTAAETAQLIIQKMTHIKEN"
     gene            34665..37313
                     /locus_tag="VH752_000316"
     CDS             34665..37313
                     /locus_tag="VH752_000316"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEQ8288693.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQGI
                     SRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKRVHSVGHSERSGAVVEPRLSTQWFVKMDELAKQAMDNQETDDRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDGWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEEAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIL
                     RLLHPIMPFVTEEIYAQYAQGSIVTVDYPVVRPAFENEAAHKGVESLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFTSNVNYIKRFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELVRLDKELAKWQKELDMVGKKLGNERFVANAKPEVVQKE
                     KDKQADYQAKYDATQERIAEMKKIKS"
     gene            37467..39875
                     /locus_tag="VH752_000317"
     CDS             37467..39875
                     /locus_tag="VH752_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HEQ8288694.1"
                     /translation="MRMILAHYDCKKDKKQSLDEHLWHVACSSRQEASIIGQGDVLFL
                     IGLYHDLGKADRTFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKNRGVDKNERMTFN
                     EMVGYVISAHHGMYDLCYYFDDAEYYGFNKFKNRINRDLDGYHYHEDIKGYALKLEKK
                     LCDYGYKDLRELIDKAFDNYQQAMSSLNWQDKSEWDYYQSCMVRLYLSLLKNADILDT
                     VNAYGLKISPMDKTERSFLKHSYLAAIEQKYASFGQPNNQLNTIRTEIAERVKERGKR
                     DSKGIYRLDLPTGAGKTNLSMRYAFHQLVHHDKSRFFYITPFLSVLEQNASEIRKVTG
                     DLGVLEHHSNVVKQANEDDDDKDSLLSAYLSDSWDSQVVLTSMVQFFQTLFKTKSANL
                     RRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYDSSEID
                     HRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVHLTDVINLILGEENSVL
                     AIFNTKKTVHNCYTMLKDITDRPVYQLSTNMCAQHRLDLIAKIKTELQNNIPIICIST
                     QLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEENISRLT
                     EIKAKKEATESILHKIGSPIDISTLNRDFFEYYYANNQGLMDYPLEDNLSIYDYLSLN
                     IYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGEAEKKLAYLEELGMSHFLS
                     AEDYQTIKSLLKELQPFTVNVRENDPLFETTKSYLNGQILVLTSEYYDTERGVKYDSA
                     SFYF"
     gene            40008..40736
                     /gene="cas5c"
                     /locus_tag="VH752_000318"
     CDS             40008..40736
                     /gene="cas5c"
                     /locus_tag="VH752_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEQ8288695.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERSSYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRECLGLLDDISQEEYETTVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
     gene            40736..42631
                     /gene="cas8c"
                     /locus_tag="VH752_000319"
     CDS             40736..42631
                     /gene="cas8c"
                     /locus_tag="VH752_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEQ8288696.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRSNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMADKQMIIFPVTADSVARSGSHPAPHPLVDKFAYYSAEMG
                     QSQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQNNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKTLHQSYISFVEANREKLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLML
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTFHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMGKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            42636..43484
                     /gene="cas7c"
                     /locus_tag="VH752_000320"
     CDS             42636..43484
                     /gene="cas7c"
                     /locus_tag="VH752_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEQ8288697.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKSIFVQANERIEDDFRSLEKRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLKGSINAYFAEKTVFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYNQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTL
                     EILEGL"
     gene            43486..44160
                     /gene="cas4"
                     /locus_tag="VH752_000321"
     CDS             43486..44160
                     /gene="cas4"
                     /locus_tag="VH752_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEQ8288698.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQV
                     LHTKADNPYIKEKRKELLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMHEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            44157..45182
                     /gene="cas1c"
                     /locus_tag="VH752_000322"
     CDS             44157..45182
                     /gene="cas1c"
                     /locus_tag="VH752_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEQ8288699.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSPALVKLCTENQINLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGRASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGSILLTENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLE
                     SYPPFMV"
     gene            45193..45486
                     /gene="cas2"
                     /locus_tag="VH752_000323"
     CDS             45193..45486
                     /gene="cas2"
                     /locus_tag="VH752_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEQ8288700.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     repeat_region   45634..45798
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=45635..45665
                     /rpt_unit_seq="gtctcacccttcatgggtgagtggattgaaa"
     gene            45958..46668
                     /gene="ccdA2"
                     /locus_tag="VH752_000324"
     CDS             45958..46668
                     /gene="ccdA2"
                     /locus_tag="VH752_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEQ8288701.1"
                     /translation="MILSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFGKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNILYAVWFRYLLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHILLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     gene            46681..47304
                     /locus_tag="VH752_000325"
     CDS             46681..47304
                     /locus_tag="VH752_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEQ8288702.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMAKKEITQDKMSMAAKKKD
                     KMSTSKDKSMMADKSSDKKMTNDGPMAPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFKKWFQGTDYKDLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKHIGYAKKSDIKKTLKGIH"
     gene            47347..48453
                     /gene="msrB"
                     /locus_tag="VH752_000326"
     CDS             47347..48453
                     /gene="msrB"
                     /locus_tag="VH752_000326"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ8288703.1"
                     /translation="MEHKWKLWLFIVGVVLLIGGASVLIQKGQAKAFSQGKPQLPAKK
                     VKSTKVDPNAPVDQKMIYLAGGCFWGVEEYFSQVDGVLDAVSGYANGRGDTTNYQLIH
                     QTGHAETVEVAYDANRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGTYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEDYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSATEIKAKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPISPDVIRYKEDKSFNMTRTEVRSRSGDSHLGHVFTDGPKD
                     QGGLRYCINSLSITFIPKADMEAKGYGYLLSSVE"
     gene            48541..49281
                     /locus_tag="VH752_000327"
     CDS             48541..49281
                     /locus_tag="VH752_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ8288704.1"
                     /translation="MYSLLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIVLTDINMPKLNGIQLAELIKQESPQSHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLRKKLELSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKDLGMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLVGVTPSQYKKGGQA"
     gene            49278..51011
                     /locus_tag="VH752_000328"
     CDS             49278..51011
                     /locus_tag="VH752_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8288705.1"
                     /translation="MKRYPLLVQLISYVFVIVIALITTLGLLYYQTSSRNIRQLIERD
                     TRQSIRQSSQFIDAYIKPLKETTSVLAKNTEIQAFASQIHQENDKQVLQLMKMVLATN
                     SDLQAAVLVTKDGRTVPTNSQLTMKTSSDMMAEPWYKAAIDRQAMPVLTPARQLSLSS
                     KKEWVVSVTQEVVDAAGHNLGVLRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVY
                     HPKKSVYSSSKEMAAMKPYLAIQNGYTKDKTSFVYQKLIPNSQWTLVGVASLDQLHRV
                     QRQIFWSFLGTGLLACLICGFATVLALRRWIRPIQQLQQVILAIQKGDRQLRAQETGS
                     PELTDLAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWM
                     AEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYE
                     VQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQHLVLTVWDNGK
                     GIEDSSLTNSQSLLARGGVGLKNVDQRLKLHYGEGYHMTIHSQSDQFTEIQLTLPKMH
                     ELMADDTQENE"
     gene            51084..52163
                     /locus_tag="VH752_000329"
     CDS             51084..52163
                     /locus_tag="VH752_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEQ8288706.1"
                     /translation="MSLASDYFSRQTPIVEKLMAYGFEKRNNGYFYNERFMEEEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLERLSVACFEVTP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVGGKWYAMIFSLLADKLDQIPER
                     LVGQTCEVMTVKVNPKDLPQLLQQEGIYPAYHMSKKNWISIILDDKVTDDKLWTLVTQ
                     SRQLVNPNGLSNPNGPDYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYM
                     TAPVKSIRYACQVLETDIPNEGYRKNPNIDKLMRLRKCQQYKDGLLSFDLMKKHGVAA
                     VRGPRRLSPQLIAFLKEKEYLKENN"
     gene            52186..52902
                     /locus_tag="VH752_000330"
     CDS             52186..52902
                     /locus_tag="VH752_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEQ8288707.1"
                     /translation="MVEFVADQSFWELFPDAKLGVVLLKDVQNQAESPEDVKQLLADS
                     HELVKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPVGAEDSDSFIGDLRLTTTDGGDDFYLIGDADNNPTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALDFIEGSAKMYLHA
                     ITSVHVLDKDNPHVPLFTEN"
     gene            complement(53068..53541)
                     /locus_tag="VH752_000331"
     CDS             complement(53068..53541)
                     /locus_tag="VH752_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ8288708.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATVCGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            53683..>54347
                     /locus_tag="VH752_000332"
     CDS             53683..>54347
                     /locus_tag="VH752_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ8288709.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEEYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDK
                     DYF"
BASE COUNT        16338 a         9959 c        11795 g        16255 t
ORIGIN      
        1 ccaatagtaa taatttttcc aagctattag cttgggcgcg tagctcaggt ggttagagcg
       61 cacgcctgat aagcgtgagg tcggtggttc gagtccactc gtgcccatta tctataatta
      121 tattattatg gtccgttggt caaggggtta agacaccgcc ttttcacggc ggtaacacgg
      181 gttcgaatcc cgtacggact atagctattt agtaacttta gttaaagtaa tcgaactaac
      241 ttagttgatt gtattttaat gataaaaagc ttaaatttgt tgtataacaa atttaagctt
      301 tttattgttt ctttttaatt acagattgag gtttaaaaga cactttacaa tgttctcaag
      361 tcatgtgttg ttgtaggttg tatgcacata ttacaaatcc cattataaaa gaaattgttt
      421 aattaatgga tagtaggatg gtatggagat cttaatgtgg ttttaatggt aattttagca
      481 gttatagtaa gacaaaaagt gacttggaaa agcttataca gacgattctt aactcccaca
      541 atatcttctg tcagatttta ttagacttct tcttggacat gagttggttt acgttttagt
      601 acaaattgag attgagctag tgtttcagtg tcaattttgt atgatttacg aacgtgcagg
      661 ctattcaatt gcttcttagc ttctaaagga ttaaaccgca ttatctgcga aaaaacctga
      721 agacgacgag aatagacatt aatcgcttta aggataatct cagggttatg gacggttttc
      781 aagtttccaa gaagacgatt gaggccgatg gtatcgtttt gtcttgaatg attccttatt
      841 ttagtggttt cattttcaat acacgacgtc aagcgtccta catactttga taaaattcta
      901 cctaaaagag gtgtcttgtc agttttgatt acaacatcaa gtgccaaaag actttgcgac
      961 ttaacaagac acttctactt taacgcaaag aaaaagtagt gagtactctc ttccgtcgga
     1021 gatttcctcg ctactaaact tagtattttt tgatactttc cttaggtagt gcttaaggca
     1081 gcgacttcct ttggaattcc atgccttatt tttgagatcc taggctcaaa aataccaagt
     1141 ggcgtcaatg ttagtgttga cgccactttt accacaacga aaaacatctg ttttggctca
     1201 ctccacacgc agaaatagga aattggatgt caaatcagca gagagctgtt catgcttttt
     1261 gagcctaaaa ctgagtttgt ctgcgttctg agcactcctt gggagtgctg gacaactctt
     1321 aacgattctt cagttcaaga taacgtttgt accaaatagt gatataggcc ttggaaaatg
     1381 gtcctttttc atgattaatc caatcaacca atattttgac attttctttt aggataaagt
     1441 ccatatcggc tgaatactgc atctcttttt tatgaatctc gtattcatca acatcaagta
     1501 atctcttttc accatccgca aagactttga catctaaatc gtaatcaatg tatttgaggg
     1561 cctcagtgtc catcatatat ggactagcta agttacagta ataagagacg ccattatctc
     1621 gaatcatggc tataatgtta aaccagtatt ttttatgaaa gtaaacgatt gcaggttcgc
     1681 gggtcaccca tcgtctaccg tcactttctg tgacaagagt gtgatcatta actccaatga
     1741 gggcattttc agttgttttt agtaccatag tatcgcgcca agtacggtgc aaactaccat
     1801 catgcttata actttgaatt gtaataaagt cgccttcttt aggtaatttc atgccttacc
     1861 aactttctac aattaaactt atcctctata gtctatcata aatgtgtgaa aaaatcactt
     1921 cttttgtcag tctataaata atttcttaac ttgcaattaa tatcgtcgct gttgtagcct
     1981 tttcggtaga gagcctgata gagcttttgc tttaaagtat aaccatcata tttgcgactg
     2041 agtttacgat attgtttgtc taattctttg tcaagaagat tttctatttc ttgattatct
     2101 tggtcaaaat taaggtgatt gaggctatgt ttagctagat cgtatgaaaa gcctttggtc
     2161 agtaatgctt gggtgatttt atctttcaag gcttttggtg gcaatttttc ttgatatttg
     2221 tcaaagagtt tttggcttac tttttgagcg agttggctaa agtcagtttg agataagata
     2281 ggatcaatgt catgacttgc aatgcctttt tgtaataatt tttgttttaa tacttgggga
     2341 cctttatcac catttaactg attttggcga atgtaggtat cagccaattt ggtgtcgtct
     2401 atccattgtt cttcttggag ttgagtgatg atgtcaggaa taatgtgttc ttcaatctca
     2461 tgcttgcgca ggtaatcagc aacttgcttg ttgctgcgtt gttgaaagga aagaaaataa
     2521 agggctaaat ttttgccgta ggacagttgg gcaaaatgtt tcatgtcttc aagctgatca
     2581 ttgtcaagga ctttatcttt actcaacata aaccgaacaa tagtatcttc tgttacataa
     2641 agggattcgt cattatccaa ttcgataagg tagaggcgtt ttttcttttc aattttagtg
     2701 attttcatac tttgattata tcaaaaatgg taaaatagaa gacgaaaata ctgctaaaaa
     2761 tgtgttgctt gtgaatgctt ttggtggtga gattaggata ttagaggaag taaggggaag
     2821 agatggtcgt aaaggttaaa cagaaaattc ctttaaaaat taagcgaatg ggaattaacg
     2881 gtgaggggat tggtttttat cagaaaactt tggtatttgt ccctggtgct ttaaagggag
     2941 aagatatttt ttgccaaatc acgtcggtta aacggaattt tgcagaggcc aaactgttga
     3001 tggtaaacaa ggcttctaag aatcgtgtca aaccagcttg ttctgtttat gaaacttgtg
     3061 gtggttgcca aatcatgcat ctagcctatc ctaaacagct agatttcaaa gatgatgtga
     3121 ttaggcaggc ccttaaaaag ttcaaaccaa ctggctatga acaatttgag attagaccga
     3181 ctttaggaat gaaaaagcca gatcactatc gagccaaatt gcagtttcag ctgcggtcat
     3241 ttggcgggac ggtcaaggct ggtttatttt cccagggaag tcatcgcttg gttcctattg
     3301 acaattgctt ggtacaggat caactaacgc aagatattat caataagatt acgcagttag
     3361 tggacaagta caagttgccc atttacaatg agcgaaagat tgctgggatt cggacgataa
     3421 tggttcgtaa ggcacaggct agtgatcagg tacagattat tgttgtgagt agcaaagaag
     3481 ttaggttagc gaggttcatt ggcgaattga ctaaggcatt tcctcaggtt aaaacggttg
     3541 ccttgaatag taatcgttct aaatcgagtg agatttatgg ggacgaaacg gaaattctat
     3601 ggggacaaga ggcgattcat gaagaagttt tagactatgg atttgcgtta tcaccgagag
     3661 ctttttacca acttaatcca cagcagacag aagttttgta tggtgaggtg gtgaaggcct
     3721 tggatgtagg gagcaaagat catattattg atgcctattg tggggttggc tcgattggct
     3781 ttgcttttgc agggaaggtc aagtctgtgc gggggatgga tattatccca gaagcgattg
     3841 aggatgcaca aaaaaatgcc aaagccatgg gctttgataa cgcttactat gaggcaggga
     3901 aggcagagga tattattcct aaatggtata aacaaggtta ccgcgcggat gccatcattg
     3961 tggacccacc aagaactggt ttagatgata aattattaaa aacgattctt cattaccagc
     4021 ctaaacagat ggtttatgtg tcttgtaata cctccacttt ggcccgcgat ttggtgcagc
     4081 taacaaaggt ttatgatgtt cactatatcc aaagtgtaga catgttcccc catacagcta
     4141 ggaccgaggc tgtcgtgaag ttacaaaaac gtgtatgatt gagtggcaat aacaactcaa
     4201 gttttataag ctcaggtaat gggtttgagc aatagcttga catagtaggg atgtcaaaaa
     4261 cggtttacta agcatagtca tattattgat aactctatgt ttattttaga aacattcatg
     4321 gttaaaaaag tttgttagtt tgtcactaag tagggtctat ttgaagagca agtacacaca
     4381 gacaatatta ggggaattga ttgttaaaaa aagctgtttt ttgctataaa ttagaaaact
     4441 gagtggtcag gaggttacat taccagaagt tattttagta tgctaatatg aacgatgata
     4501 tagaggattt atcctgattt aatcttttaa gctgtaagaa acctttgcaa aaactgtagg
     4561 ctgtgtgagt ctttatgtgt tagatattaa agataaatgc aacacttttt tataatgaga
     4621 tcaacaactg ataaatctga gttgataacc agtaaaatga tcggaatgta gaaaagttac
     4681 ttaatcaagc ttttcgtaat ttaactgtta gaatgacaga acttatgata aaataaagaa
     4741 tatgattaaa atgaaaaagc tcagtttatc tattacggag ttcgaaatga aaagaaaatc
     4801 gagatgtgat aacatatttt gaacgagaag gagaaggata aatgttagaa gattttcttc
     4861 aatttttagg atttatattt ttagatatta ttgaaattat gttaacgtta aagcttttta
     4921 gctttgtaag tgcaattccg cttcgcctga aaaatatttt ttatttatca cttagtatgg
     4981 ttttgtttca agtagttttt tgggctttct ttccagacca tttcatcctt gatgttgtga
     5041 tgttggctca gtttcttttc tttgcgctta tagccctata ctatggtaaa tcaataaaag
     5101 ctaagttttt aatgttttat gccttttttc cactagtgtc tattagtttg gttaagcgat
     5161 ttattgtatt ttttgttatg ccattgtttg gtatgcctta ttcagttgtt aaacataata
     5221 cactactgat ttacagcatt acttgcttta gtattttttt gatttatcgc tgcattcaag
     5281 tttttcattt cgatttttca acctggcgcc aatattttca atcacataga gctagtaaac
     5341 tattagtgtt tactaactct tcgatggctc tatattactt gtgtgtccaa gggatagatg
     5401 tgatgtcgcc ttctttatca ggacttgcta caacgactgc tcgttcaatc atagtgctct
     5461 tttatttcat tttgtttctt actttattaa ttcatttaga gcgttatgta aaacaaaact
     5521 ctattgaggc aattgtgcaa caaaaagaat atcgtgagct gattaattat agtcagcacc
     5581 ttggattgct gtatcaagat attcaagagc ttaggcagct attaactacc gtgtccagtc
     5641 gtcttaagat tggaatcgaa caaaatgata tctctattgt gagacttact tatgaaggta
     5701 tcttgaatgc tgaaaagaat aatgctaaag atgacagact tgatttaact tgtttagata
     5761 aattacaagt tgaagcaatc agacatattg ttttagctaa attaattgag gcaaaaaata
     5821 agaagcttaa ggttgaggta tcgatcccta attgtattgc aacgtttttt ctagaagtag
     5881 tagatttcac taagctattg tcatttttgt tagataatgc tatagaaatg agtttggaga
     5941 caaagcaacc ttgcttatca atagcatttc tggatcaaaa ccataaactt gtcatagtca
     6001 ttcaaagcag tactaaacaa ggacaagatg atagtcaaag cgtgtttgct ataccggctt
     6061 tgaaaaaaag agatgactgg caatttgact taaggaatgt cacgaccatt ttaaatcgtt
     6121 atgactatct cacaattagc tcgcagattc atgatggcat tttaacccag ttaatagaaa
     6181 tagctaagcc tgactgatgt taaaggtatg aataatcact caaaatcaac ttaagagtct
     6241 gagacaatag tcccagactc tttttgaata tgttaatcta ttactaatga taatagttta
     6301 ttgtttcttc caagctagag ttaaaatttc cgctttgcca agttggctgg gtgtgctttg
     6361 cttgctgtct ttttctaaca aattagtgac cttggcattg tagtttggca ggcttaggct
     6421 aaagtcacaa gttttatcgt ttgagagatt atagctgcgc gtgataaggg cattgtctgc
     6481 taagcgacgt ttgaaagctg tgagggcaac ttggtcgttt gtacccgtca aatagctata
     6541 ttctgcggct aatgttccct cgtgttggtt tgtttgtgtg gtaataacag ggacttggcc
     6601 ttcttgagct cgccagtagc tggcaaattg tgtttgctta gtgatgcttt caaaactata
     6661 agaaaggctg tgcttgccga ggcattgggc ttcaggagta gggaaatacc cccagtcgcc
     6721 catttcgcca acagaacgta agagagtgat ggcaatggtg ttggtatctg gtaagatctc
     6781 atattcgttg aggccatagt taccaatagt gactccattt tcaccatcaa agagactcac
     6841 aaagcattct tggtgctgtg ggttacttgg attcttccaa aaggtggcat ctggatgatt
     6901 tggacgtttg acagtttcaa aaatactgtc agctagatga tggtctgttt taaggtgcgt
     6961 tgggaatagg acgcgcaagc gatgatttgt catttggtta tcaaaacgtg tggtaaattg
     7021 gaggcgagga ttgtttttct ccatacggat aagggttgtt aaggttagct cagccttttc
     7081 ttgtgaacgt cttgcttggc gttctgtgat gtcaatgaca gcttccatct cagcctgtaa
     7141 gcggtcatcg gcggagacag gaatggtaaa ggtttgctgg atttcaagtt cagcaacttg
     7201 aatagtgttg ctgcgaacag aaatatgagt tacgccttgg tccgcataga aaggttggtc
     7261 atgagttggt tggcgagaaa tatattcatt tccaatatcg ccacaatctt caaaccgcaa
     7321 caggtcttga tagattagtc cagattgttt atcggtgatg gttagacggc catcggtttg
     7381 tatcataact tttagaaacc cattttcaag gcactgatta ctgtcatcgt agagggaatc
     7441 ggtttctgaa ggagttgttt catttcctag ctttaatgct aaggttttcc aagacatggc
     7501 tgggagagtt attggtagcc gtaatctcac tttgatggcg aaataaggtt cgcggaagga
     7561 tctctcgggc aaatcgtagt caaaagcgat gcttgtgcct aaaatttctg cttcgggact
     7621 cacttgtcca ctagtgtcaa taatttggaa agattgggag agtcctgcca aatattcttg
     7681 agcttcttgg taaacctctt taggaaagcg ttgaccaaaa tgatatgttt tccaggtcag
     7741 gctgagctca gcaacacttg ttttggaatg accgctggta ttaaataaga cgaagggttg
     7801 gctatccatt ggaaaatccc tggtatcaat ggcgtcagca atttgcttag cagcttcttt
     7861 tgctaaatag tgtccgactt cataggcttt ttcaaagcgc gtcatcattt cccgatgaac
     7921 gctatcaaca ctacaaccac agatagaatc atgagggtga ttttgcatga gggttttcca
     7981 agcgtaacgc agttggtcgt gagggtaggt acttgtcacc tcataagcca ttgctgctaa
     8041 gggttcggtg atgttttcga gttggcgaga gactctggta ttagcctgtt tgaggtaaat
     8101 acgagcagaa gccgtgttag ctagggtata ccagccatcg gtttcttgac tggtaatctc
     8161 tccttggacg gttgaaaggt tctctggtaa atcatctgcg agatcagcca agtaatcttc
     8221 aaagcaggaa tgcacaaatt cgtagtcagg atagagttgg ttggctaagg cgattgcctt
     8281 ggtgacatca agttgtacgg gttgatgatc acacccgttc atcatcagaa ggtgcttggt
     8341 tgaggcgaag cgttcggcgt cagcaagttt tttatcccaa aaaagacgcg cctcagcttc
     8401 tgttgtcggg atctcattgc cattgctgta ccagttggcg aagaggagtc caagaatacg
     8461 actgtcatct ggtccttgcc aactgatttc agagaattgg gagctgtatt tttcactggt
     8521 atctacttga ttgttaaatc cagttggacg aatgccacga ccaaaggcag cagcttgtag
     8581 gccggctttt aacatcagct gtggtgtttg tcccatattt ccaaaggtat ctggaaaata
     8641 accaagtggc acactagcgc cccatttgtc acaatcctcc ttgccaatga gcatattgcg
     8701 cacattggac tcgctgctgg tcaaaaaatc gtcctgtaag atatagaaag gtccgatacg
     8761 gagttttccc gaagcaatgg cttgtctaat ctcaggttct cgttcggggc gtacttttaa
     8821 ataatcatct aggataatgg tttgaccatc caaatgaaaa ctatgaaaat taggatttgt
     8881 ttgaaaaact tctaacaggt catctattaa gttaatcaga cgcatgtggt gttgctcgta
     8941 agccatgtac cactcgcgat cccagtgact gtgtgaaata atatgtactt ttttggttgc
     9001 catagtggcc tcactttcta aggagcaatg ctaaagccaa gatagtcaag aagtagttca
     9061 cagaacatca tgttagccca agagaaccac tcacgggtgt attgataagg atcattgaca
     9121 tcgaaaccct cgtgcattaa gtgggtacca gcatctgttg ccacaagcaa atcaagcatc
     9181 tcttttttac tgtcttggtc aagagcggtc aatccttgta aggcaagggc gatatgccag
     9241 atataatttt ctggtgtatg ggaagaaccg atgcctgctg ctgcatttcc ctgataataa
     9301 taagggtttt cttggctgaa aatggtgcgg cgcgttgcca gatagatagg gtcatcttta
     9361 gcacaaaaac caaggtaagg tgcagcgagg aggcttggaa tattagcatc atccatgatg
     9421 gaaaactgtc ccagaccatc cacttcataa gcgtaaatgg tttctccctt gctgttttga
     9481 acaagggcat ggtctgcaat acctgattga atagcttctt ttagctgctt ggcacgagta
     9541 gctattggtt cattgtgaaa aaggttgtta tataaatctt ctagatagga aagcacaacc
     9601 actgcaaaca tatttgaagg aatgaggtaa ccatattgac aagcatcatc acttggtcta
     9661 aagcctgacc aggtcattcc tgttggtgcg acaggacttc cttttccagc atgtgttaag
     9721 gtatcttctt tgcgccaagt atcgcgttca aacaagtaag gagactgggc gtggtcttgc
     9781 tcaactgtcc ataggtcaag aatgagttct accccacgat gaaaatcgtc attaaactga
     9841 tcggtgcttc ctgtttcacg atagagcaga taagccagtt gaatagggta gcaaagggaa
     9901 tcaatctcat atttgcgttc ccaaatccaa gggttcattt gggtatgatc tgtttggtgt
     9961 cctttttcgt tagcttcttc gttgaaggca ttggcatagg gatcgataca aatgtagcgg
    10021 aattggcgtt tgaccagacc ggcaataatc tttctaattt cttcatcttc cttggcgagg
    10081 aagaggtaag gttttatttg cgcagtggaa tcacgtaacc acatggcagg aatatcgcca
    10141 gttaaaagaa agctagtgcc atcttcgtag gtttttaggg ttgtctctaa ggtattggta
    10201 aagctggttt tgaaattaag agcccaatca gggtgttggt ctccacaaag gctttccacc
    10261 ttggccataa attggctgac gctagttggg attggtcttg tcatgtcata tcctccaaaa
    10321 tgatatatca tttatcttcg gtttcattat aaatgataag ttaaactttg tctataagtt
    10381 tttgaaaagg tttacaactc ttttttgaca tgttatactg ataacaggag taaagaatga
    10441 caaaattatt atacaaaaag atatatcatt tcattaaaga gcagatagca tcaggtcgtt
    10501 tgcaaattgg tgaccgccta cctactgaaa aagaattgtc agaacagttc tcggttagcc
    10561 gtattacttc taagcgagcg cttgtagaac tggagcaaga aggcttgatt acgagaagtc
    10621 gtgggaaagg aagcttcgta gctaaaaagc aagtgacgtc ccctgaagcg aataaggatt
    10681 tattgttgat tcttcctttt gcctcagatt atgaattggg ggattatgct aagggaatta
    10741 tgacctccat tgctgaaaca gggtatcggt tgatggtgca actggccagt acggtaaggt
    10801 tagacaccct gtcagactat gctgggatta tttattatcc tgaagatgtc aatcacagta
    10861 ttgatttcct gttttactgc gaccatcatc acattccgct agtcttgttg gataaatctc
    10921 tggacctttt tcagtttccg gctgttgtgg cggataataa aggcggtgcc tatcaattga
    10981 ctcagcactt gattgagcaa ggctgcgacc agatttggtt tgtggcaaca ggctcttttg
    11041 gagaagtatc atccgttaga gaccgttatt tgggttactt agcggcaatg gcagaatcgt
    11101 ctctaaaatc ccattatttt cctaagacag agactgaaac aacagatgct tatttggacc
    11161 gtttggtgaa aaccttatcg gagacgacag ccactaaaag ggggcttgtc gtggaaaacg
    11221 attggttggc tattcagctg attcaaaaaa tcacacaggt aggcttatct attcctgatc
    11281 aagtcgctat tgtaggcttt gataatagtc aagcctcccg tttgttgagt cccaaattaa
    11341 gcacagcagc tcaagatctt taccaaatgg ggcaagaagc agctaaagtc ttgttgcaga
    11401 ttattcatga gccagaccag gcagtggtgt cttgccagtt acctgttcaa gtatttatca
    11461 gagaaagtag tcactaaaaa agatgttact agcatgagct ggttagctca ggtaaaggag
    11521 accagaaatg accatttatc acgccttaaa ggattatcaa gaagtcatga caagaggaga
    11581 ctatcttgtc tttgacacgc cattgacttg ccgatttatt ggccattttt tccgttttga
    11641 aaatcaaaaa gccctgctgg cagaattggc aacgtcaaag tattttcagt ggattgaaga
    11701 aggccaagca gaggtaacga tgaaacactt ttttaaccgt cagctggcta aggatgcctt
    11761 taccctgaag gtttcagaag ataaggaaat cattattgaa agccaaaacc ttagaggttt
    11821 tcgttacgcc caagaagctt tgcttaaagt catgaccttc aaaggggata aactttattt
    11881 gcccattgtt tctgtcaagc acagcccgtc atttcctatg cggggaataa ttgaaggctt
    11941 ttatggaaca ccgtggacac gagaggaacg aatcgattgc ctacgtttta ttggaaataa
    12001 aaggatgaat acttacatgt atgctccgaa agatgatgat taccagcgaa aactgtggcg
    12061 cgatctttat cctgaggatt gggtgactta tttcaaagag ctacttgcag tagccaagga
    12121 agaagggatt gatttttggt atatgattag tccgggactt gattttgatt ataccaagga
    12181 agctgactat cagttgctct atcaaaaatt gcaacaactt ctagcgttag gtgtctgtca
    12241 ttttgggctt ttgctggatg atattgacta ccaaattgta gatgctgttg agagacgatt
    12301 caaaaagact gcctatgccc aggcccactt ggcaactgag gttcatcact ttttaaacca
    12361 acaacatgcg gcgccagaat tggtgatctg tccgacagaa tacgacaacc accatgattc
    12421 tatctatttg caagagctgt cagaaaggat tcctaaagaa gtcgcctttt tctggacagg
    12481 cccaagcacg ctagcgagtc aaatatcgca agcagatatt gaaactatgg cagcagtata
    12541 tcaacggcct atcattattt gggacaatat tcctgttaat gattatcaaa aagatccaga
    12601 acgtctcttt ttaacccctt ttgctaatcg ctcgccattt ttatgccagc cagactatca
    12661 ggtcaagggg attgtttcta accctatgat tagttgggaa ttatctaagc tgaccttgac
    12721 agatatgagc cattatctgt gggatgctaa ccgttatcag cctagtcatt cttggctaga
    12781 aaccttaaca gactatacag aagatactga attagcccta gctttacagg catttgcttg
    12841 gcataatggc aatcgtcatc tgcacaggga ccttcctttt gaagtggaag aagctctcct
    12901 ggctaaggat gtatcaacgt tatcagcttg ggtagctgaa ttggtcgaat gtgtcaatac
    12961 gttgagaaag ctagataagc cagcgtttca acaagctatt gctccttggt ttgaacgggt
    13021 ggcaaaagat caggtgtttt ggcaggctat gttaaatcag gagcctcagt tggaaacact
    13081 ctatgctgac ttacaagaag ataaacaccg cattggaagt gacattccaa gtcgttacta
    13141 tcgcatctac tatcaacagc aagataaact aacagctaat caaggtcaag ttacccaagc
    13201 aagacctgaa gattatgctt agtcatacga gctagaaagg aatgatatgt tagcatttcc
    13261 aaaagagttt tggtggggag gagccacttc aggccctcaa agtgaggggc gttttgctaa
    13321 gcagcaccgc aatctctttg attattggta tgaagaggag cctgacttgt tttacgatta
    13381 cgtgggacct gatacggctt cggatgccta tcatcagatt gaatctgatt tgaccttgtt
    13441 agcttcacta ggtcacaatt cctaccgcac ctccattcag tggacacgtt tgattgatga
    13501 ttttgagcaa gcgacgatta atcctgatgg ccttgcttat tataatcgag tgattgatgc
    13561 ttgcttggcc aatggtatcc gtcctgttat taatttgcat cattttgacc tgccaattgc
    13621 cctctatcaa gcctatggtg gctgggaatc aaagcatgtt gtggacttat ttgtggcctt
    13681 tagtaaggtt tgttttcaag aattcggtga tcgggttaag gattggtttg tccacaatga
    13741 acctatggtc gttgtggaag gctcttattt aatgcaattt cattaccctg ctatcgtgga
    13801 tgggaaaaaa gcagtccagg tcgcctataa cctagcccta gccacagcca aggttattca
    13861 agcttacagg caaggtccag cagaacttag tgatgggcgt attggaacca ttctcaatct
    13921 gacgccagcc tacccagcta gtcaatcaga agcggacatg gctgctgcgc attttgcaga
    13981 actttggaac aatgacttgt ttatggaagc tgctgttcat gggaaattcc cagaagaatt
    14041 ggttgcggta ttgaaaaaag atggggtttt gtggcaatca acgccagaag aattggctct
    14101 tattgctgag aatagagtgg attatttggg attgaatttt taccacccta agcgcgtgaa
    14161 ggctccggat gtcattccag tgattagccc atcttggagt cctgaatggt attatgatcc
    14221 ctacctgatg cctggccgcc gcatgaacgt ggataagggg tgggagatct atcctgaggc
    14281 tgtctatgat attgccatca agatgcgaga ccattacgac aatattcctt ggttcttatc
    14341 tgaaaatggt gtagggattt caggagaaga gcgttaccgt gatgaaactg gtcagattca
    14401 agatgattat cgcatccaat ttttgaaaga acatttgacc tacctccaca aaggaataga
    14461 aacaggctcg aattgttttg gataccatgt atggacgcca attgatggct ggtcttggct
    14521 caatgcctac aaaaaccgct atggccttgt ggaaaataat atccataccc aagtgcgacg
    14581 acctaaagct tcagcttatt ggtttaaaaa agtagctact cataaccgct tgatttcatt
    14641 agaagtaatg gaggagtttg gaggctctgc ttctcacctc agtgattgaa cgtgtgaccg
    14701 tctcctttga gacaagtgaa taatgatgtt accaatgatt aaacaagggc ctttttcctc
    14761 atgttaagag gatcgaggct cttgtatttc tttggaaagt gttggtattt gccgaactag
    14821 atataagcgt tctgtctggt ttggcatcat ctatccacct tgggtaattt ggctcttcaa
    14881 ttctttagag tatcttggct aaggatgctt gtttttcatc ttggtatttt aggattttct
    14941 ttcatatttt tggaccagat aaaagcccct catggtttct agtgactgtt tgttatgtca
    15001 ctgtccacga taaggggact atatgagtga gttattaagt gaggctttgc ttattggcga
    15061 taactagcat agcttctaat agctcgagca ttaactgatg accaatgcct ttaacagaag
    15121 cgtcagcaac aacgacatca acaaagggac caggcatcag gctgcgttca tcagcagaga
    15181 gtcatttagg cagaaagcca gtatcgttgt agctaatcag caatcctttt aggatgtctt
    15241 tgtagatgtc agaggcgatt agggacaata ggggataaga gggatttagc ggtccatggt
    15301 taggcctcct tgtgttcttg gtaaaaatca atggcagctc caagaagatt ggcttcgttt
    15361 ttaaagtgac aaatagctaa ttggggtcta aggtcggaga tttcgacggc ttggtagagt
    15421 ttgtctagtt cagcttcaat cgctgacagt agaaagtcag cttgggaaac accacccccg
    15481 attaaaatca gttgaggatc aaaggcgtgt tgaatgttaa aaataccgat ggctaaactt
    15541 tggagaaaga cctgcctttc tttgattgct aggtggtctc cttgttctgc gagggcaagg
    15601 acagctttgc cagtataatc ttttccgtta ttgacgattg cactatagcg cttggccata
    15661 tttaccactg tccctaactg actaaaggtt tggtagcggt cgttcatgat catgaaacca
    15721 aattcgcccc caaataagtg ggcaccatgg taaatcttgc catcaatgac caagcttcct
    15781 ccaactccag taccaagaac gagcatggct aggctactag cgccttttcc tgcccctaag
    15841 gtagcttcag ctaaggcggc acaattggca tcattttcga tagaaatagg gtaatgtagc
    15901 cgttcttcta agacctcttg tattttaaaa tggtgaatgt agggaatagc gctggcacct
    15961 tcgataatac ctttttcctt gttaaccgct ccaggagcac taacggcaat acctgagaaa
    16021 tggtaagcag agtaacgagc aacttcttgg tcgagtaatt ggtaaaactt ttccagacta
    16081 gagggagtgg gaaagctgct ttgctggctt aattggccat tgtgacagag ggcaaattta
    16141 agggaagtgc ctcctatatc aatacataac aagctcatga ggtgttctcc tttattatag
    16201 tagaatagac gatcatgtct catcttagta tgacagattt aaacaacaat gaaaaggctt
    16261 tctaagtgat tctatacttt gtgttatcat acctataatt tgttgctttc aaataatgaa
    16321 agcgcttgat atactgtaac tataacgtga ttaacacaaa gggaggttgg gaaatgacaa
    16381 gacaaaccaa caaaaagaca tccttttgga aaaatgtgat taaataccgg gccttattat
    16441 taatggtatt accaggtttc atctggttca tctttttctt ttatattcca gttttggcca
    16501 atgtggttgc ttttaaagat tttcattatt cagcaggggg ctttatggaa agtttgaaag
    16561 agagcccttg ggttggtttg gctaacttca aatacctctt tgcttccaaa gatgcttggt
    16621 tgattaccag aaataccatc gcttacaatg tgattttctt actctttaat gttttctttg
    16681 cgattgcttt tgccattatc atgagtgagt tacgaaataa gcgaacggtt aaggtctatc
    16741 acaccatgtc cttattgcct tatttcttat catgggtggt tattgaatac tttgtttctg
    16801 cctttttaaa tacggataaa ggtttcatta atcagctcct gactggaagt ggagcagatc
    16861 caatcaagtg gtattccaat cccacttggt ggcccttgat tttactcttt atgagtgtct
    16921 ggaaagggct aggctacaat agcattatct actatgcttc agtcaaaggg atttctgata
    16981 cttattatga agctgctatg gtggatggtg caagcaaatg gcaacaaatt aggaacatca
    17041 ccattccaca attattgcca atgatgtcta tcctattgat cattaatatt ggtaatatct
    17101 ttaaatctga tttcggactt ttctacgtta ttcccaaaaa ttcaggccca ctttatgacg
    17161 tgaccagtgt gttagatacc tatgtctaca atgccttgac tgctacagga gatattggga
    17221 tggcatcggc agctagcctt tatcagtcag tagttgggac cagcatcttg ttagtcacca
    17281 atgcgattgt tcgtcgcatg gaccctgatg cagccttgtt ttaggaggaa aacatggaga
    17341 aaaagaaaaa agtcgaaaag gttaatgtta gaacctttga tcgaaaaacc aatgctatct
    17401 ttaatattct gattggtctg tttgccatct cttgtatcat tccttttatc ttcgtgatta
    17461 ttatttcctt cactgatgaa agttacttga ttaatcatgg gtatagtttt tttccagatg
    17521 tctggtcaac taaggcttac cagtatattt ttcaaggagc catgtcccat agaatcatga
    17581 ggtcatttgg gatatccgtg tttattacgg tggtgggaac ctttattaac acgaccatga
    17641 catcaaccta tgcttatgcg atttcaagac cttatttccc atacagacgt ttttttactg
    17701 tttatgcact tatcaccatg ctctttgcac cagggatggt tgctaattac ttggtggtca
    17761 gcaatctcct tcatttgaag gatacggttt gggctttgat tttaccaatg gctctgggcc
    17821 catttggcat cttggtcatg agaacgttct ttaaaaagac agttccagat agcattattg
    17881 agtcggctcg tatggatggg gctagtgaat ggatgatttt tatgaaaatt gttctccctt
    17941 tggctgttcc agggattgct accatcagtt tattttctgc cttaacttat tggaatgatt
    18001 ggtttaacgc cttgctttat gtgcaaagtg aaaacctcta cccaatgcag tacttactga
    18061 tgaaaattca gagcaaccta caggccttgg cacaaaatgc tggcatgagt gcacaaatgg
    18121 cagatagctt agcatcgctg ccaaaagaat cagttcgtat ggctatcgtg gttattgcaa
    18181 ccttgccgat tgccttgact tacccattct tccaaaagta ctttgtcggt ggtttgacca
    18241 ttggtggggt gaaggaatag gacttaagga actggtcaca agaccagatt ttatctaatc
    18301 attagtaaca aaagaaaaca ttttttagga ggcttcactt atgaaaaagt ggcaaaaaat
    18361 cgtgtgtgtg actggaactg tgcttgcagc gtctagttta gcagcctgcg aaagcaagtc
    18421 agcatcaaag gatagtgatg tcaaattatt gatgtaccaa gttggtgaca aacctgataa
    18481 cttcgatgaa ttgatgacaa ttgctaacaa gcgcatcaaa gaaaaaacag gtgcaacggt
    18541 tgaccttcaa tacatcggtt ggggggactg ggatgataaa atgagcacca tcattgcctc
    18601 tggtgaaaac tacgacattg cttttgccaa taattatgtg gtcaatgcac aaaaaggtgc
    18661 ttttgctgat ttgacaacgt taatgccaaa atacgctaag aaaacgtata aaaacttaga
    18721 cccagcctat attaaaggaa atactattga cggtaaactc tatgccttcc cagtagatgc
    18781 caacgtttat gcccaacaga tgctttcttt caataaagaa ctagtggaca aatatggcct
    18841 tgacatttca aacattaagt cctatgcaga tgctgaaaat gtcttgaaac aattccacga
    18901 aaaagaacca aatacagcag cttttgctat tggtcaagtc tttagtatgt caggtgacta
    18961 tgactaccca ttaaccaaaa accaaccctt tgctgtgaaa attgatgaag gtaagccaac
    19021 catcattaat cagtatgaag atgagtcctt taaaaacaat ctccgcttga tgcacaaatg
    19081 gtataaagaa ggtttgattc caacagatgc agcgaccaat acagaaggtt atccccttga
    19141 aggaaacact tggtttatgc gtgaagaaac ccaaggtcct atggactatg gcgatactat
    19201 cttgaccaat gctgcaggaa aagacatcgt gtctcgtcca ttgactaaac cgctaaaaac
    19261 cacatcacaa gcacaaatgg caaactttgt ggtatcaagc gtatctaaaa acaaagaaaa
    19321 agcagttgaa gtcctttctc ttcttaatag cgatcccgaa ttgttaaatg ggcttgttta
    19381 tggtgtcgaa ggcaaagctt gggaaaaaat tggcgacaag aaaatcaaac tgctcgatgg
    19441 ctatcaacca aaaatgcaca tgggtgcttg gaatactggt aacaataaaa tcctttacac
    19501 tcaagaatct gttactgacg acatgattgc taaacgtgac caatcaatca aggatgctaa
    19561 agaatctcca atccttggtt tcactgttga cacaaaagtt atcaaaacgg aattgtcaaa
    19621 tatttcaaat gtcatgaacc gatataaagc aagtatcaat actggtacag tggatcctga
    19681 cgaagccctt ccaaaacttc ttgcagacct caaaggtgct ggttgggaca aggtgcaaaa
    19741 agaagttcaa aaacaattgg acgactttgt agctaaagac aagtaaggca ttcattttct
    19801 tgattataag ttgagggctg ggaaatctca gctcttttct tctgatgagt ggaaaaaatg
    19861 gatcatcaaa taagtggaag ttataaaggg agaaaaatta atgtaaataa tgttactact
    19921 cttagtaaag cggtttccaa aagcctataa agtgtgctat attagaagag tctaaactat
    19981 taatcagagt aaaactaata ccagcatctt attgggggtg ctaaaactag tctcgtagct
    20041 gctggtccct gaaaccgcta taagtgttaa caagaaaaag aggactatca tatgaatggc
    20101 attgaaaaaa cctgcttttt aatttggaaa atgatccaat tgaccctcat ttttcatctc
    20161 ttgtccttag ctggactgat tgtatttggg gtggggccag cctggcaaac cattgtcacc
    20221 ctatttttaa aaacatctca agaagaaaac cactattctt tgaaaagtgc tgttcaactt
    20281 tggaaatctt atttcaaaga agctaatcta agatttggtc tttttgccct gactttcctt
    20341 tttttaacct ttaatttgca ttgggcagta cagtttccat ccttgttttg gtttaccgtg
    20401 tctttcttga ttgtaatagc catggtttgg ctaaccatga cctatcttta catggttttt
    20461 tatgcagtga gctatgagat tagcctttgg aataatatga aattagcctt tatcagcgtc
    20521 tttttaagtt tcaaatcctt tttactcatg ctgagcgttt tgctgggaat tatcttagtg
    20581 acttggcaat acaaaggtct ttatctcttt ttaaccttcg gagctctagt cttttgcttg
    20641 gactttgtca ctaaggctaa tcgtcgacta gtagatggag tgattgatga gtgttaaaac
    20701 attgggaagg tatgtgacta accactttaa gcagggtttt ttgattaatc gactgatgaa
    20761 attatatagc ctgctaattg taagtttttt cactttagca gctattggct tgagcagtta
    20821 ttctattagt agtacttata aaagggtgga tgctgaggcg cagatgcgac tggaggagac
    20881 tctaggtaga ttgcagtcac aaaacgatat taccctacgg gttttagacc agttggttgg
    20941 ccgttcagaa gattaccata acctctatca atatatgacg ttaaccccaa accaatattt
    21001 ttcaaacgtt tttgaagact gggaaaaagg aaaagacacg gtcctatttt ctgaagaggt
    21061 gaggcgtctt tttgacttgt accctgatgt gacttctatg accctactgc tagaggacag
    21121 tagtgcttat ctgtacgccg atagggtcat taagacgggt cgtttgcttt atagcaagcc
    21181 tgaaaaagta accggaaatg ttttagttcg ctccattcga aatccagaat caggagatgt
    21241 gacaggtcgt ctttacctga cctttgacaa accaccgact ttggtagaga cacagcagga
    21301 tcactattta gctacttttg cttttgatta ctatggccgc aaactcttcc atcaaggagg
    21361 tcggagattt cctcatttag aggctgaggt taaaaaggct atcaaggcag atcgggcagc
    21421 tgatcttagt aatctcagca aggcttatcg gatgcagtac aatcgctcgg gagacctctt
    21481 agcttatgtg gctgtcagaa aatcctattt attagcggaa gctgtcagga cagtttttgt
    21541 gcatggcctt gtttctctac tcttagcttg gctgttattg cagttgctct ttagggtttt
    21601 ccgaaattat atccagcagg tttctgaaat tacggatact gttgaaatgg ttgcagcagg
    21661 cgatttgtct ttaaccatcg acaacagcca catggaattg gaactgtatc atatctcaga
    21721 agccattaat cagatgttgg ccagcattaa agcttatatt gacgaagttt atgtgttaga
    21781 ggtagagcaa cgagatgccc aaatgagagc tctgcagtct caaatcaacc ctcatttttt
    21841 atacaacacg ttagagtata ttcggatgta cgcccttagt tgtcaacaag aagaattagc
    21901 agatgtcatt tatgcctttg cgagtctgct tcgcaacaat attagccaag ataagatgac
    21961 caccttaaaa gaagaactgg ctttttgtga aaaatacatt tacctctacc aaatgcggta
    22021 tccagatagc ttcgcttatc atgtaaagat tgatgagagt attgctgact tagccattcc
    22081 taaatttgtc attcaacctc tcgttgaaaa ttattttgta catgggattg attatagtcg
    22141 caatgacaat gcactaagca tcaaggcatt agatgagacg gatcatctct tgattcaggt
    22201 gcttgataat ggacgtggta ttagtcaaga gcgcttagca gatatggaaa aaaggcttca
    22261 agagcaccaa acaacaggca atagctctat tggtttgcaa aatgtttacc tccgtctttt
    22321 ccatcatttt cgagacaggg tttcttggtc catggctaag gagccaaatg gtggctttat
    22381 tattcaaatt aggattagaa aggatgcttg atgtataagg tactattagt tgacgatgaa
    22441 tacatgattt tacaagggct caccatgatt attgattggc aggcacttgg atttgaagtg
    22501 gttcaaacag caagatcagg caaggaagcc ttagcctatt tgactcagta cccagtggat
    22561 gtcatgattt cagatgttac catgcctgga atgacaggac tggatttaat agaagcagct
    22621 aaaacctatc atcctcagct acaaaccttg attttatctg gttatcaaga attctcctat
    22681 gtgcaaaagg ctatggaatt agaaaccaag gggtatttgc taaaacccgt ggataaggca
    22741 gaattacagg ctaaaatgaa acaatttaaa gactgcttgg atgcccagca ggcagaatca
    22801 atccggcaag aagcctatca tgacagcctt ttgacccttt ggcttacaga tgaattgagt
    22861 gagaaagaat ttcagcagtt aagtcaaggc ttgccagcgg ctgctttaat tggttttaca
    22921 gtgctgtatt tggactgtca agagtggcaa gcagccatcg atgcttattt tgaacaagaa
    22981 ggtcagcctt tttacctcaa aaaagaagaa gataagaagg tttatctggc tgtcctactt
    23041 ggtaaaactg accaagccaa ggcatttacc tatgacctac aaaaacgatt ctcaagaaac
    23101 atgcatcaac ttattttggg agaaacagtt gatgactggg aaaatgttta tgaaagctac
    23161 aatcaggtgc ttaagagcct cttttacaat tgtgaagtgt caccaattag agcaaaagat
    23221 aagtccaaaa ttgaactacc agagtcccgt ttgcagttct ttgcttttaa caaggcgttg
    23281 atgattgggg acgaacctac aatcttgtct aaattagaag ctatttttga cgagatgaag
    23341 accttgaact tttcaccaga ggatgtgaag catgtttcgt tcttgttgtt ttctgacatt
    23401 taccgtcaat ttcccatttt agataaaatg acttatctgt ccatggtaaa aactattcat
    23461 gacagccaat ccattgatcg cattttgagg gaactaaaaa aagtgttgga tgtcaccaat
    23521 cagaacaatt cccctgaaaa acgctattca gatttagtta gtgaaaccat tgattgcatt
    23581 cgcaaggaat accatcaaga attaaccctt aaagctatcg ctgataggtt acatgttaat
    23641 ggggtttatt tagggcaatg ctttaaaaat gaaaccgagc gtagttttac ccagtactta
    23701 aatcatgtta ggattcaaaa agcgcaacag ttattgcttt acaccaacca atccatcaat
    23761 gaaattgctt atgaaacagg ttacaatacc aatcattatt ttatcaagat gtttaaaaag
    23821 ttgaatggtt tatcgccaaa agagttcaga gatcgttaca aggacaatta ccaagccatc
    23881 aagggcgacc agtaacatta aacaggagat tgatatgaca ccagatagca aggagcagac
    23941 agtgactggc taccaatacc actatattga tcaagaagga agaaagcagc cctttaatca
    24001 aggctggcgt tttcttatgg cagatgtggc gtgtgctcag gccccttctt ttgatgattc
    24061 gaactggcag gtgattcatt taccacatga tttcagtctt acgcagcctt acacaagaaa
    24121 tggtgaagct gaaagtgctt ataaattagg tggtgtcggt tggtatcggc attatctcgt
    24181 gttagatgag gttttagctg gttgtcatgt tgccattact tttgagggtt cctatatgga
    24241 aacggaaatt tatgttaatg ggcaattcat tggcaaacac ttaaatggct atcaggaatt
    24301 tacctatgat atctctgatg tagtgacttt tggagctgaa aatcttctgg ctgtccgggt
    24361 tgaaaacaag gttcccagtt ctcgttggta ttcaggcagt ggtctttatc gtgaagtgag
    24421 tttgtctgtt cttccccagc ttcattttgt ggcagaccag gtagcaatga ccttggctga
    24481 tactgctgtg caggagaaag ggcaacagaa agttgattta cgctttgccc ttaatcagcc
    24541 tattcagact tgccactacc aattgagcct ctgtttatgg gaacaatcac attgctcaaa
    24601 agacaaaaaa ctcctttatc aagaaacaga agtgcctcta gctgatttgg ctttccaaag
    24661 acagtatggt cttacactgt ctttggaaga actccaattg tggtcaccag ataatcctca
    24721 cctctacgat ttagaactca ctctttacta tcaaggtcag gtcattgatt gcttttgcct
    24781 tgaaactggt tttcgtcaac tgacttttac ggccaatcag gggctttttg tgaatggcag
    24841 agctgtcaaa ttaaagggag tttgtttgca tcatgaccaa ggaggcctag gagcttgtgc
    24901 ttacgaagat gccctagcca gacaattagt cctgttgaaa gacatggggg ctaataccat
    24961 tcgtagcacc cataacccaa gtagtcccaa acttaggcaa ctagccaatc gtctcggctt
    25021 ttttgtgatt gaagaagcct ttgacacctg gacctatgcc aaaaatggaa acgttaatga
    25081 cttttcaaac tatttccatc agactatcgg aactgaaaat gcaaactatc ttcaaagagt
    25141 gaggtcacct gaaacgagct gggcccagta tagtattgag gccatggtct ggtctgccaa
    25201 gaatgacccg tcagtgttga tgtggtcgat tggtaatgaa ttgatggaag gtttttcagc
    25261 cgatgtcagt cattacccag agctgacaag acaaatgtgc caatggattg ctgctattga
    25321 cacctcacgt cccattacct ttggggacaa taaattaaaa gaagctgact tttgctggca
    25381 tgaggaagtg tcgcaaatgg caactcttct tagtcaatta gaccatcccc agggactcat
    25441 tgggttaaac tatgctaatg gaaaagacta tgataagctt catgaggagc actctgattg
    25501 gctgttgtat ggttctgaaa cagtttcagc cattaccagc agggcttatt ataaagagac
    25561 aaaaaaagtt cttgactccg gttatcacct aacctcttat gatcatgcta aggtcgattg
    25621 gggtgctttc gctagccagg cttggtatga taccattacc cgtgattttg ttgctggcga
    25681 atgcgtttgg acaggctttg attatttagg agaacctaca ccctggaaca agatcgacag
    25741 cggtgttgta ggcctttggc cttctcctaa aaatgcctac tttggcattt tagacacggc
    25801 aggctttcca aaagatagct attattttta ccaaagtcag tgggtgcagg ggcagaccac
    25861 cttgcacctg ttaccagtct ggcagaagga ccagctttgt ttcgatgagc agggattggt
    25921 ggaggttgtg gtttatagca atgctgccag tgtgcaactg atgtttgaag atgagcaggg
    25981 caacttaaca gattatggaa ggaaggcatt tcatacttat agcacgccaa cgggacacac
    26041 ttatcagctt taccaaggag ctgatgctgc taagaatcct cacgagaatc tttacctgac
    26101 ttggcgagtg ccctatcaaa aagggttgct aagggcagtg gcctacgaca tttctggtaa
    26161 aagcattcca aaaaccagtg gacgttctca ggttagaacc tatggttcag tggcaaagct
    26221 atcttggaaa gcctttgaag cacctattga cgctccttgg gaattgcttt atctggattt
    26281 atcacttttg gatagccaag gtgaattggt cagccatgct caagattggc taaaggttca
    26341 agtagaagga ccagcccgtt tgttggctct tgataatggg aatccgactg accacacccc
    26401 ttatcaggag cctctacgcc aggcatatgg cggaaaatta ttagccattt tagccctcac
    26461 tggagaggcg ggacacatta aggtcacggc aaaagcctcg cagcaactta gcagtgtttt
    26521 tcagacacaa gtagcctgcc gtgcccccaa agaacactta gtgacactac ctttagagaa
    26581 atatcagttt aaaaagagcc gactgacaag tcaacaagaa attggtcaat cttgtctaaa
    26641 gaccacacaa ttcctttctg ttttggagaa caaagacacc cagcaaaaag caggtaaggc
    26701 tagcacacct cacattctta tcactagtta ccaacactat ttatctctgc ccttgcaggt
    26761 ttgttctcag gcatttgatt ttaaaaaagg cgatgatatc ttcttgcctc agtggctcca
    26821 agtcaaagat gcccaaggtt cttttgtgaa aaaagctttt ccaatgacat ggtgcctaaa
    26881 agagaaaatc tttgccaagc ctattcttgt cacgggctat gtttcttgtt ttgggcaatc
    26941 aataccggtt gaggcaaggc ttaacctagc acaagctagc aaaagacggc accaggatct
    27001 cagtttagtc gccaagcaat ccctggccac aagtcaaaaa aggctaactt attgctacca
    27061 atatgatacg gtgcaagcca taggagccat tagcttgcaa aatcacacag gcacatctct
    27121 tagtgtttct gtggcaatca gttatggtag tcagagctct attaagcaag aaaccttgat
    27181 gctagctgat agccacttga gccagttaga agaggttatt ttcttcgatg atattttgac
    27241 tgttttgtat ctggcagtga tccttgaagt cattgaagga cctgacctaa ctgaagacag
    27301 tattcaaatt ggcctttggg atttagcatg aagcatgagt aaaagaagac ttctttggca
    27361 ccaggtctga tgaattctgt aaaataaggt ataataaaaa acaaataggc gtcctttatt
    27421 acgtgaaagg cgcctttaca aggttgaaat ggaaagtgag atttctaaat gtcagaacgt
    27481 ttatcaggcc acaccctgtt ggtctctttg cttgctaccc ctattcgtca tagtttgtct
    27541 cccaaaatgc acaatgaagc ctatgctaaa ctggggctgg attatgccta ccttgctttt
    27601 gaagtaggga cagagcaatt agcagatgcc gtgcaaggga ttcgtgcctt aggaattcgt
    27661 ggctccaatg tgtctatgcc aaataaagaa gctattttac ccttattgga tgacttgtcc
    27721 ccagctgctg agttggtcgg tgcagtcaat acggttgtca ataaggatgg taaaggacat
    27781 ctcgtggggc atattacaga cggcatagga gcgctcagag ccttggctga cgaaggagtg
    27841 tcggtaaaga acaaaatcat taccctagca ggagttggtg gggctgggaa agctatcgct
    27901 gtccagctcg cttttgacgg cgccaaagag gttcgtttgt ttaaccgaca agctacacgt
    27961 ctaagtagcg ttcaaaagct tgtgacaaaa ctgaaccagc taacaaggac taaagtaacc
    28021 cttcaagact tggaagatca gactgccttt aaggaggcca tcagggaatc tcatctattt
    28081 attgatgcta caagtgtagg aatgaagcct ttagaaaatc taagtttgat taccgacccc
    28141 gagctcattc gcccagactt ggtggtcttt gacatcgtgt acagcccagc agaaaccaag
    28201 ttactagcct ttgcccgtca acatggcgcc caaaaagtca ttaatgggct agggatggtg
    28261 ctttatcaag gagcagaagc ttttaaatta atcactggtc aagacatgcc agttgacgct
    28321 attaaacctc ttttaggtga tgagtaacac tttttaagta agcttaatta tgatatatta
    28381 ataagagatg gagcagtcat catgggctgc taaaattcct tttgcgagag ctgtttttaa
    28441 gagcaaaaag cttatttgaa gagccttatc cctaacgtga tacaagtcat agttcctttg
    28501 aggtctgcca gataacctac cgttttttta gaaaagaagc tcttataaaa ggaaaagttc
    28561 aatgaataaa ggagaatagc aatggtaaca aaggaagttg ggcatgactt tttagcacgt
    28621 ctaggaaaaa aacgcttaag acctggcggt gttgaggcga caaactggtt gattgaacaa
    28681 ggacaattct ctcaggattg tcacgtttta gaggttgcct gcaatcgctg cacgacagca
    28741 atagagttag ccaaaacctt cggttgtcag attgatgccg ttgacttaaa ccccaaggtt
    28801 gtcaaagaag cacaagaaag ggttgctgtt gaggggttaa cccgtcacat tacagtatcc
    28861 caagccaatg ctttatcact accatttcca gataaccatt ttgatattgt gattaatgaa
    28921 gccatgttaa ccatgttgaa cgcccaagcc aaggagaagg cactcaatga atattggcgc
    28981 gtgttaaaac caggcgggcg acttttgaca catgacgtag cttatgaaga tccaaaaaca
    29041 agacaagtac ttgacgagtt acgtcaaacg attcatgtca atgttgctcc cttgtcacta
    29101 acaggttggc aagccttata tcaagaacac ggtttttctc aagtgactta tcaccatggg
    29161 aaaatgtctt tgatgacacc tcgtggaatg attaaggatg aaggtcttat gaacaccctg
    29221 aacattatga gacgaggcct taagaaagag aacagacaac agtttttaaa gatgcgtcgt
    29281 ttttttacca gaacaggcaa aaatctctgc tatattgctg taaaaagtgt aaaataaacc
    29341 ttagaccaac ctgtccatac aactaaacag accatagagg agttcgaaga tgtcttatat
    29401 caataatatt gagcacgcga aggttcttga tttgacgcaa gaagtaatga ttgagcaaga
    29461 tcaaatgtta agtagaacac tagtccaaag gcaagactta ggaataacag ttttttcctt
    29521 agataagggc caagaaattg ggcgacactc atcaccagga gatgccatgg ttactatctt
    29581 aagtggctta gcagaaatca ccattgatca agagacctac cgggttgctg aaggtcaaac
    29641 cattgtgatg ccagctggga ttcctcatgc cttatatgca gtagaagcct ttcaaatgct
    29701 tttagtagta gtaaaaccgg aggtttaaca tgattgaaaa tctctctcaa ctggtgcaat
    29761 atcatgatca tcaggtggct agccgatcct tgagccgtct actgggatta tctcaatcta
    29821 ttgttttata tgccatggcc caaggagaaa gcattagtca agagactagt ccaaaagata
    29881 aagtaatact tgttttagaa ggtcagctca ttttcgattt agaggatcaa aaacaggtcc
    29941 taactcaaga aagtctcata gccatacctg cccaaaaagt ccatcattta gaagctaaaa
    30001 ccgattgtaa actattacaa gtgcttttag attaagggct tattgtcaac tgtattgggt
    30061 ggcctatgga aaagcaaaag agaagatgac gctcatcttc tctttttacg tattcaaagc
    30121 gatgacaata cgaattttaa ggcttggctt tctcatctcc agattatgcc ctttagcttg
    30181 gtttagttta gaattcacta aaaaaacctt aatgagtttc ctatctcctt ataggtttct
    30241 cattaaggct ttttagacga aataattgaa cgacaggcaa aagaaagatc tttttacgag
    30301 tgataccgtt ctcctatagt ttcataaatc ctctttttga tgttgctgtt agttattctt
    30361 agctaaaagt tatattgtta tcaagacttg tcttgtagta acatgtttta ggagttccag
    30421 gataccgtta ttctcttagc aagcttaact agctagagtg gagtcactat ttataataaa
    30481 ctgtcgagct tctcgacaaa ttggtgatcg tctttgttaa tggctagggt ggtaaaaccg
    30541 gtttctgtgg gtaatataaa gataatatgt tgctcagaat tcttcttatc tgttttaaag
    30601 acgtcaaaga gggatgtcgc agagacatca gctctttcaa gtgttgttgg taactggtaa
    30661 cgttcaaaaa cttgaaggag gctatccaag tcctcttttg gcaagagtcc tttggaaaca
    30721 gccagtttag catcaaccac cataccgata gcaatggctt caccgtgatg gtaaaaatca
    30781 tggtggcaga gcgattcaat cgcgtggccc aaggtatgcc caaaatttaa agacatgcgc
    30841 aaaccatttt caaattcatc ttgttcaaca atctgttttt tattggaaat ggattggtaa
    30901 atcagacgtt caagtccctg cttatcggtt tctttttgaa aatcacgtaa ctgctgaaaa
    30961 agcggtctat catgaatgaa gccgattttg agcatctcac tgatgccgca ggagaattcc
    31021 cgttgcggta aggtttccaa aaaggtggtt gaaataatga tagcttctgg agggtaaatg
    31081 ctgccaatca tattggttag acttttaaaa tggactccaa ctttgccccc gatactgcta
    31141 tccacctgac taagtaaagt cgttgggatt tgaatgtagg gaataccacg gtaataggtt
    31201 gcagcgacaa aaccacccag atccccaaca acaccaccac caatagtaac aatcatatct
    31261 tttttggaaa aattttcagc gatgagctga tcgtagatag cactgacacg ttccaaagat
    31321 ttggtttgac ctcccggggg gcagacatgg acaaaagcgt tgtattgctg agcaaattct
    31381 tcaaagagag tctgatacaa gtggtagacc gtttgatcgg tgataaacaa gagtttacgc
    31441 tgcttgcgct caccaaggca atctgctaag gtttttaaaa catggtcggt aaacaggata
    31501 tcataatcct tgacccgaga atggacatga agtgtttgtg gcataagcac tcctttcatc
    31561 agtactaggt aacagagata acgcagtgat atttagagct taatcttgcc aaagttcctg
    31621 ccccaactgt tctaattgct tataatcaat ttgctgggca gcatctgaca gcgcatggtc
    31681 gggatcagga tgaacctcca tcatgatgcc attggcaccg acagccttcg ctatcttagc
    31741 agcaggtaag agtaaatctc gtctgcctgt cgaatgagac acgtccacaa taatcggtaa
    31801 atctgtcttt tgttgaataa tagggactgc cataatatca agcatatttc tagtctccac
    31861 atcgtagcca cgaacgccac gttcgcataa gataatgtta gattttccag tgtcttgtaa
    31921 gtaggaaagg gcgcccaaat attcctcgat agtagccatc aagcctcgtt taaacaaaat
    31981 aggcttatcg atgtgtgaca aggtttttaa aaattcaaaa ttttgcatgt tgcgggcacc
    32041 aacctgaatg acatcgagat agtcataggc ttcttctaat tggcgctcgg acatgatttc
    32101 actgaccgat agtaagccaa attcctgaca aacctcatgc aagtaacgaa ttccttgcag
    32161 gccaaggcct tgaaaagaag cagcagatgt tcttggttta taggcgcctc ctctaaaata
    32221 gttgtaacca agttttttgg cacttgaagc tgctaatcta atgtgatcat aagattcaat
    32281 ggaacaaggt ccaacaataa aattattctt agaacaagtc ttattttgaa agtcagacat
    32341 aatgtcaatc attcgtttga ttcctttact aattctgtat ggcgtgctct atgggataac
    32401 gtcttattta ggaatacaaa atctcgttgt tatccatgaa gcacccttag cctgtgaaat
    32461 gataccataa acaagatgaa atagcaagta aaccctagat tgtctatgtt cttagtcatt
    32521 tttattaaaa aaggcttact agctataaaa aatactgccc taaatcttat taggacagta
    32581 ttttttatag atgccaccac cgagaatcga actcggaacg aagcattacc attgctttat
    32641 tataccattt aactacagcg gctacctttt tataatacta taagttatta attgatgcaa
    32701 gcttttctta aaaatatttc cttaattgtt tatcatcaaa atgagagtac cagttattaa
    32761 taacatggca caagaggcct gccttatggt ataatggtaa agatgatttc agaaagagga
    32821 agctaggcat gtcttatgaa aaagaatttt taaaagattt tgaagactgg gttaaaactc
    32881 agattcaagt caatcagcta gccatggcaa ccagtcaaga agttgcccaa gaagatggcg
    32941 atgaaagggc taaagatgcc tttattcgtt atgaaagtaa gttagacgcc tacgaatttt
    33001 tactaggtaa atttgacaat tataaaaatg gtaaagcctt tcacgatatt ccagatgagt
    33061 tatttggagc aaggcattat taaggacaag gagacacgaa atagtggcaa aacaaaaaaa
    33121 tcgtaaaaaa gcattgaagt tagaaatgaa gcgtaatggc ttgttaaaaa aagcaggtca
    33181 agtttttgat aaggcagttg aaagtgttga aacagctgtg gataaaacca tttctgctgg
    33241 taaaaatctt gtggaaaaag gcagccaaac ggttgaaaac ctgacagcaa gcaaggaaag
    33301 acttactctt gatgagtttg cagggcagcc agttgtagct ggtatccgct ctgaccttgt
    33361 cgaaacccta tatgcagaag gcatccattc agcgcaagct tttaaggagt ggacagaaaa
    33421 agacctcctt gctcttaagg gcattggccc agcaacggtt aaaaaattgg tcgaaaatgg
    33481 cgcaagcttt aaaaaatagt catcttctcg ttgcttaatg gctataaatt tgttagaata
    33541 ttagcaatca gaggtgcatt gagttgatct gttacagaga gcggtggtgt tgagaaatcc
    33601 gcacagatgt caaggctagt agctgaggat cagaaatcag atatgaaaag tagcagaccc
    33661 attttatggt ttgaaacagt ggtggtaccg cggaaatcgt aacacgaaat tcgtccctgt
    33721 ctaggttatc taggcaggga ttatttggtt tcatttgaaa gaagatgatg gcttattgtt
    33781 aaaatgttgt tagaagtata taatagaaaa actttgtcca aagtggaaac agaacggctg
    33841 ctattacgtc aacgaaaagg ggacaaggca ttgttccaga agcggcttca gctctgattg
    33901 aaattggttt taccttactt gatttgcata aaattgagct gggttgctac gactacaaca
    33961 agcaaagtca ggcagtggct cgcaaattag gctttacctt agaagccaat gcccgtgacc
    34021 gtaaggacgt tcaaggtcgc cgctgtggcg acatgcgttt tgggttactg agaagtgagt
    34081 gggaggagca gaaacaaaaa tgaaccttat catcattggt gctcaagcgt cgggcaagat
    34141 gaccattggg caagaagttg ccaggcagac aggcatgact ttgtttcata atcacgattc
    34201 tattgacttt gttttacggt ttatgccttg gtctcaagag agcactgcct tgattgaacg
    34261 tatccgtttt gctttttttg aaacctttgc taagacaggt caagacatga tttttactat
    34321 tgtaattgac ttcaatgatc caaatgatgt agcaatgctt gaaaagattc aggctgtttt
    34381 tcagtcttat gaccaagagg ttctctttgt cgaattaaaa acagatatag aggaacgtct
    34441 taaacgcaat cgcacagaaa atcgtctcaa acataaacct cttaaacgca acatcgagtg
    34501 gtcagaacaa gatattcaat ccacgatggc atatgctgtt tttaatccag aagaaccacc
    34561 caaaacgctc acgcattacc aaaaaatcaa taatacccag ctcaccgctg ctgaaacagc
    34621 gcagcttatt atccaaaaaa tgactcatat caaggagaac taagatgaca gaactatcac
    34681 ctaaatacaa tcctgccgag gttgaggcag gtcgctatca aaaatggctt gatgcagatg
    34741 ttttcaagcc atcaggagac caaaaagcaa aaccgtattc tatcgtgatt ccaccgccaa
    34801 atgtgactgg taagttgcac ttaggacacg cctgggacac aacgcttcaa gatattatca
    34861 ttcgtcaaaa acgcatgcag ggctttgata cgctttggtt gccagggatg gaccatgcag
    34921 ggattgcgac acaggctaaa gtagaggagc gtctgcgcga gcaggggatt tctcgttacg
    34981 accttggtcg tgacaaattc ctagacaagg tctgggagtg gaaagacgaa tatgccacta
    35041 cgattaaaga gcagtggggc aaaatggggc tttctgtgga ctacagccgt gagcgcttca
    35101 cccttgatga gggcttgtca aaagcagttc gcaaggtttt tgttgacctc tacaaaaaag
    35161 gctggattta ccgtggtgaa ttcatcatca actgggatcc tgctgccaga actgcccttt
    35221 ctgacatcga ggttatccac aaggatgttg agggagcttt ctatcacatg aactatatgt
    35281 tggaagatgg ctcacgcgcc cttcaggtgg caacaacgcg accagaaacc atgtttggag
    35341 acgttgccgt tgccgtaaat ccagaagatc ctcgttacaa agatttgatt ggcaaaaatg
    35401 ttattttacc aatcgtgaat aaattgattc caatcgtggg tgacgagcat gccgatcctg
    35461 aatttgggac tggagttgtg aaaatcacgc ctgcccatga tcctaacgac ttcgaggttg
    35521 gtcagcgtca taacttacca caagtcaatg tcatgaacga tgacggtacc atgaatgagt
    35581 tagctggcga ctttgcaggc atggaccgtt ttgaagctcg tcaagctacc gttgctaaac
    35641 ttgaagaact tggcgcccta gtaaacatcg aaaaacgtgt tcactcagta ggtcactcag
    35701 aacgttcagg tgcagtcgtt gagcctcgtc tatccaccca atggtttgta aaaatggatg
    35761 agcttgctaa acaagctatg gacaaccaag aaacagatga ccgagtagat ttctacccac
    35821 cacgctttaa tgataccttc cttcaatgga tggaaaatgt ccatgactgg gtaatctcgc
    35881 gtcagctttg gtgggggcac caaattcctg cttggtacaa tgcagaaggt gagatttacg
    35941 ttggcgaaga agcaccagaa ggtgatggct ggactcaaga tgaagatgtt cttgacactt
    36001 ggttctcatc tgctttatgg ccgttctcaa ctatggggtg gcctgacaca gatgtagaag
    36061 atttcaaacg ttatttccca acctcaacct tagtaacagg ttatgacatc attttcttct
    36121 gggtgtcacg tatgattttc caatcgcttg aatttaccgg gcgtcagcca ttccaaaatg
    36181 tcctgattca cggtcttatt cgtgacgaag aaggacgcaa aatgtctaaa tcattgggga
    36241 acgggattga tcctatggat gtcattgaga agtacggtgc cgatagctta cgttggttcc
    36301 tctcaaacgg ctctgcccca ggacaagacg tgcgtttctc atacgaaaaa atggacgcct
    36361 cttggaattt cattaataaa atttggaaca tttctcgcta tatcctcatg aataacgaag
    36421 ggttgacctt ggaagaagct gaaagtaatg tggctaaggt agctgcctca gaagctggaa
    36481 atgtgactga ccagtggatt ctccacaatc tcaatgaaac aatcgctaaa gttacggaaa
    36541 actttgacaa gtttgagttt ggtgtggcag gacacattct ttacaacttt atttgggaag
    36601 aatttgccaa ctggtatgtg gaattgacca aggaagtgct atatagcgac aacgaagctg
    36661 agaaagttat cacgcgctct gtccttcttt atactttgga taagatttta cgtctccttc
    36721 acccaatcat gccatttgta acagaagaaa tttacgctca atatgcccaa ggttctattg
    36781 tgacggtgga ctatcctgtg gttcgcccag cttttgaaaa tgaagctgcg cataaaggtg
    36841 tggaaagtct gaaagacctg attcgtgcag ttcgtaatgc gcgcgctgaa gttaacgtgg
    36901 caccatcaaa accaatcacc attttggtga agacagcaga tagtgagttg gaagacttct
    36961 tcaccagcaa cgtgaactac atcaaacgtt tcaccaatcc tgaaaaatta gaaatcagct
    37021 cagccattgc tgcgccagag ctggctatga ccagcatcat cacaggtgct gaaatctact
    37081 tgccactagc agatttgctt aacgtggaag aagaactcgt tcgcctagat aaagaactag
    37141 ctaaatggca aaaagaattg gacatggtcg gcaaaaaact cggaaacgaa cgctttgtag
    37201 ccaacgccaa accagaagtc gtccaaaaag aaaaagacaa acaagctgac taccaagcta
    37261 aatacgacgc aacccaagaa cggattgcag agatgaagaa aattaaatca tagtgtttag
    37321 aacgcagttg aaaaactgtg ttcttttctt actaatagag atattagatt gaagtttaat
    37381 tatgagaaaa acgctttctt tatttaaaga gttatgttac aatactatta caatcatcgt
    37441 acaaatagtg ttattttgag gggcatatga gaatgatttt agcacactat gactgtaaaa
    37501 aagataaaaa gcaatcttta gatgagcatt tatggcatgt ggcctgttct agtcgacagg
    37561 aagcatctat aattggtcaa ggagatgtgc tttttttaat tggtctttac cacgacctgg
    37621 gcaaagctga tcgaaccttt caagataaat tattaaataa tccaaatcgg catgttgatc
    37681 actcttatgc aggggcaaaa tacttatgtt ctattattgg gcctcatcta aaaaaccgag
    37741 gggttgataa aaatgagaga atgacattca acgaaatggt ggggtatgtc atctctgctc
    37801 atcatgggat gtatgattta tgctactatt ttgacgatgc tgaatattat ggctttaata
    37861 agtttaaaaa tcgtatcaat agagacttag atggttatca ctatcatgaa gatattaaag
    37921 ggtacgctct aaaattagaa aaaaaattat gtgattatgg ctacaaagat ttaagggagc
    37981 ttattgataa agcttttgat aattaccaac aagccatgtc ttccttaaac tggcaagata
    38041 agagtgagtg ggattattat cagtcttgta tggtgagact ttacttgtca ctcttaaaaa
    38101 acgctgatat tttggacaca gtaaatgcct atggccttaa gataagtcct atggataaaa
    38161 cagagcgatc ctttctaaaa cactcctatt tagcggccat tgaacaaaaa tatgctagct
    38221 ttggacagcc aaacaatcag ttgaacacta ttcggacaga aatcgctgag cgtgttaaag
    38281 aaagaggtaa acgagattcc aaggggattt atcgcttaga tttaccgaca ggagctggca
    38341 agactaatct tagtatgcgt tatgcgtttc accaattagt tcatcacgac aaatcaaggt
    38401 ttttttacat aacgcccttt ctttcggttc ttgaacaaaa tgcttccgaa attagaaaag
    38461 ttacaggtga ccttggcgtc ctagaacacc attccaatgt ggtgaaacag gctaatgaag
    38521 atgatgatga caaggacagt ttattgtcag cttatcttag tgatagctgg gacagtcaag
    38581 tagtcttgac ttctatggtt caatttttcc aaacactttt caaaacaaaa tcagctaatc
    38641 tgagacgttt ttcaagtttg attaatagtg ttgtgattct agatgaagtt caatccctgc
    38701 ctattgaagt caccactttg tttaatttaa cgatgaattt tttaaataaa gttatggata
    38761 caaccatcgt tctttgcaca gcaacacaac ctgcttatga ttcttcagag attgaccatc
    38821 gtatctgtta tggagggaac ttgggagaat tagctgaaat cgttgagtta acgattgaag
    38881 aaaaacagat tttttcaagg acagagctta gaaaatttga tgatagtgat cagaaagttc
    38941 acttgactga tgttattaat cttattctag gtgaggaaaa ctcagttctt gctattttta
    39001 atacgaaaaa aacggttcat aactgctata ctatgctaaa agacattact gatagaccgg
    39061 tctatcagct ttcgacaaat atgtgtgcgc agcatagact tgacttgatt gctaagatca
    39121 aaacggagtt acaaaataat atccctatta tttgtattag cacgcaatta attgaagcag
    39181 gtgtagatgt tgattttcat cgcgtcattc gttcctactc agggattgat tctattgttc
    39241 aggctgctgg acggtgtaac cgagaaggca aacgagataa agggcaagtc actcttgtca
    39301 atctgaccaa tgaagaggaa aatatttcta ggctgacaga aataaaagct aaaaaagaag
    39361 ccacagaatc tattcttcat aagattgggt ctccaattga tatctcaact ttaaaccgtg
    39421 acttttttga gtattattat gccaataatc agggactgat ggattatcct ttggaagaca
    39481 acctatcaat ctacgactat ttaagcctta atatttatca gacggcaaat aaaaagttca
    39541 aaggtaagtt aaaacaagct tttaaaacag caggagccaa aatgaacctc atcaataatg
    39601 atatgatagg aattctcgta ccttatggcg aagctgagaa aaaattggct tatttagaag
    39661 aattgggtat gtctcacttt ttatcagcag aagattatca aacgataaaa tcattactaa
    39721 aagagttaca accttttacg gttaatgtcc gcgagaacga tcctctcttt gagacaacaa
    39781 aatcttatct aaatggtcag attctggttt tgacgtcgga gtattatgac acggaaagag
    39841 gagttaaata cgattcagct agcttttact tctaactcaa aacgaaagca gattaacaaa
    39901 aggttgttag aggaccttgt taacctgcca atcatcatta ataattatta tcaatttaga
    39961 ctatttaata aaattagatt acaaaaaaac agaaggagga aagtagcttg tacagatcta
    40021 gagacttcta cgtgagagta agtggtcagc gagctctttt tacaaatcca gccacaaaag
    40081 ggggatcgga acgctcatcc tattcggttc cgactagaca ggcactgaat ggtatcgttg
    40141 atgccatcta ttataagccg acctttacta atatcgtcac agaggttaag gttattaacc
    40201 agattcaaac cgaattacag ggtgtcaggg ctctgttaca tgattatagt gcagatttaa
    40261 gttatgtatc ctatttgagt gatgttgttt atctgatcaa gtttcatttt gtttggaatg
    40321 aagatagaaa agatttgaac tcagatagac ttccagctaa acatgaagcc attatggagc
    40381 gttctattcg taaaggggga cgtcgagatg tgtttttggg tacaagagaa tgtttagggc
    40441 ttttagatga tatcagccaa gaagagtatg agactactgt gtcgtattat aatggtgtca
    40501 atatcgactt gggaatcatg ttccattcct ttgcctatcc gaaggacaaa aagacaccat
    40561 taaaatcata ctttacaaag actgtgatga aaaatggagt cattacgttt aaagcacagt
    40621 ctgaatgcga tattgttaac acgctttcta gttatgcttt taaagcacca gaggagataa
    40681 aatcggttaa cgatgaatgc atggagtatg atgccatgga gaaaggagaa aactgatgga
    40741 tttttttact tctctcttga agacttatga aaaagcagag ctagcagact tggttgatca
    40801 ccaaaaaaga agtaatgagc cggttttact gccgatttat catacgagtt taaagtcaaa
    40861 tggcaaaaat atcatttcag tgaaacttga caaagatggc cagtttcaca aggcagaatt
    40921 tatggcagat aagcaaatga ttatttttcc tgtaacggct gattctgttg ctaggtcagg
    40981 tagtcatcct gcaccgcatc ccctagtcga taaatttgct tattatagtg ctgaaatggg
    41041 gcagtctcag tatgattctt ttcataagca acttaataac tggattgatt attgtgagga
    41101 gggtgacgtc aagaggtttt taaccattgt tcagcaattt attttgaaac cagaatttct
    41161 gacactgatt ctcgattctt taattggtcc tgattatcaa aataatcaat taaaagtcac
    41221 attttgtgat gccactggaa aagaaaaatt aattgattta tcagcttgct ttttagaatt
    41281 ttcaattgat cagttccagg gctttaaaaa tgaatcggtt tcgacattta aaactttaca
    41341 ccaatcctat atttcttttg ttgaagccaa tcgtgaaaag cttggtattt gtaatattag
    41401 tggacgagag gaacagctta ccgataagca tagaggtttg atggggaatg ctaaaatcat
    41461 ctctgttagt aataaaagag aagcttataa aggacgtttt agagaacgtg aagatgtttt
    41521 tagtgttggc tatgaaactt ccgaaaagat tcatttaatg ctcaagtacc ttttagaaaa
    41581 taaaaatacc agtacttggt tagggtcttc tcaatattta atcaactggt ttagtgatga
    41641 tttaacaaat gatagtcggt tggatatcgt atcaccaatc tttgatgacg gacttgaaga
    41701 agatgatgac gacgatacgc ctcctgttat aacattagca actgaagaca ataaaagaat
    41761 tggtaaatca ttcatcaagg gacaaaaatt atttgctaat gatgccactt actacgttgc
    41821 tattttgaat aaaaccagca atgggcggat tgctttaaaa tattttcgtc agcttcaagc
    41881 gtcccaatta ctcaccaatc ttaacaagtg gcaggaaaca tacagttggg agtcgcgatc
    41941 taagtttgga aaaagtcgct taagaacccc tacttttcat gacatcctta atgtgtccta
    42001 cggggttgat agggatcgct tccttgaatt agataatgat aacttcaaaa gtgatcaaat
    42061 tcaaaagtta gtggcaagtt tgattgatgg taaaccgatg ccacagtcca ttgtcaaaaa
    42121 gttaggtaac aatgttaaag aacgacatcg ttaccgtaag cactggtatc aagttgagca
    42181 ggtctgctta gcaattttac acaaacaaaa tggggaggaa ttttcaccga tgctagatca
    42241 taccaatcaa aatcgttcct atctttttgg acgattatta gcaatttttg aattaatcga
    42301 gaccttgcgt tatggcttgg atggaaacaa taacgaccgt attaccaatg ctgaacgtta
    42361 ttggacagcc tatactggac agccaacaaa attgatgatg ttattggaaa ataaaattaa
    42421 gccttacgaa gaaccattga aattaaatcg tcgtggcagt tggatgaaat tagaaaaaga
    42481 aaaagaagag attttagaac tgttaaatcc tctgttagaa acagaaacaa tgggaaaacc
    42541 cttagattac cgctttattt ttgggtatta tgctgagaaa aactattact atacaaaaca
    42601 aaacacggaa gtaacagaaa gtgaggagta aaaagatgtt ggaacacaaa attgatttta
    42661 tggtaactct tgaagtgaaa gaagcaaatg caaatggtga tcccttaaat ggaaacatgc
    42721 ctcgtacaga tgccaaagga tatggtgtga tgagtgatgt ctccattaaa cgtaagattc
    42781 gtaatcgttt gcaagatatg gggaagtcta tttttgtgca agctaatgag cgtattgaag
    42841 atgattttcg ttcactggaa aaacgctttt cgcaacattt tacagctaag acacctgaca
    42901 aagaaattga agaaaaagca aatgcattat ggtttgatgt tcgtgctttt ggacaagttt
    42961 ttacttatct gaaaaaatca attggggtgc gtggaccagt ttccatcagt atggctaagt
    43021 ccttggagcc aattgtcatt tccagccttc aaattacgcg tagtaccaat ggtatggaag
    43081 ctaagaataa tagtggccgc tcttctgata cgatggggac aaaacatttt gtagattatg
    43141 gtgtgtatgt acttaaaggt tctatcaatg cttattttgc tgaaaagact gttttttctc
    43201 aggaagatgc tgaggctatt aaagaagttt tggttagctt gtttgaaaat gatgcgtcgt
    43261 ctgcacgtcc ggaaggctct atgcgagttt gtgaagtctt ttggtttacg cattcaagca
    43321 aattgggaaa tgtttcaagt gcgcgtgtct ttgacttgtt agagtataat caatcaatag
    43381 aagaaaaaag cacttatgac gcttatcaga ttcatctaaa tcaagaaaaa ttggctaaat
    43441 atgaagcgaa agggttaacg cttgaaatcc tagaaggact ctagtatggt ctatgccgaa
    43501 gatgattatt taatgctatc aggtattcag catttccaat tttgtaaacg tcaatgggcg
    43561 ttgatccata ttgagcaaca atggcttgat aatgaagcga cagcgcatgg acaggtttta
    43621 catactaaag cagataaccc ttacattaaa gaaaaacgaa aagagctttt ggtctcacgt
    43681 gctatgccca tttcttctgc agaacttgga ctttcaggaa ttatggatgt tgtggaattt
    43741 tataaagatg atcaaggtgt gtctttgagg ggaaaacgtg ggaaatggtt accaaaagtt
    43801 gtggaataca agcgcggaaa acctaaaaaa gataccagag atattgtcca gttggtggct
    43861 cagaccatgt gtttagaaga aacgctagac tgcgacatta acgaaggttg tctttattac
    43921 catagtgtca atcaaagagt gattgttcct atgacatcag ctttgcgtca agaagtgaag
    43981 gaattagccg cagagatgca tgaggtttat cagagtcaaa tgctacctaa agcagcttat
    44041 tttaaaaact gtcagctttg ttctttagtc gatatttgta agcccaggtt gagtaaaaaa
    44101 acaaggagtg tgtcgcgtta catcaatgag gctatgacca gtgaggagat ggacctatga
    44161 agaagttgct aaataccttg tatttgacgc aagaagattt ttatgtcact aaagagggcg
    44221 ataacattgt tatcaagcaa gaaggtaagg ttctcaaacg gtttccgttt cggattattg
    44281 acggtattgt ctgtttttct tatttgggtg tgtcgcctgc tttggtgaag ttatgtacgg
    44341 agaatcagat taatttatcg tttcatacac cacaagggcg tttttgtggt cgctatattg
    44401 gttcaaccaa tgggaatgtg ttgttgcgta gagaacatta tcgtttatct gatcgtgagg
    44461 aatctttgga atacgcaaag cggtttattt tggctaaaat ttccaattca aggaaatact
    44521 tgctacgctt taaacgagat catcgtcaac agattgatac caagcttttt gaggctgtta
    44581 atgacgaatt gatatgggct ttagagatgg ttcaggcagc agataataaa gactctttaa
    44641 gagggattga aggccaagct gctaatcagt attttcgcat atttaatgac ctggtgttga
    44701 cggacaaaaa aacgttttac ttccaaggtc ggagtaaacg accaccctta gattgtgtta
    44761 atgccctctt gtcttttggt tacagtttac tgacctttga atgtcaatct gccttggaag
    44821 ctgtcggatt agacagttac gttggtttct ttcacacgga tcgtcctggg cgtgctagtt
    44881 tagcgcttga tttagttgaa gagttccgct catatattgt agatcgtttt gtcttttcat
    44941 taattaataa aggacaactt cagaaaaaac actttgaggt taaagaaaat ggtagtattt
    45001 tattgacgga aaatggcaga gctattttta ttgatttgtg gcagaagcgt aagcatactg
    45061 aggtagaaca tccttttaca aaagagaaag taaaacttat gttattaccc tatgtacaag
    45121 cgcagctttt agctaaggcc atacgaggag atttagaaag ctatccacct tttatggttt
    45181 aggagatgtt atatgatggt tttagtcact tatgatgtaa atacggaaac acctgctggt
    45241 agaaaaagat tgcgtcatgt tgccaaactc tgtgtggact atgggcaacg tgttcaaaat
    45301 tctgtttttg aatgttctgt gacacccgca gaatttgtgg atataaagca ccgcttaaca
    45361 caaatcattg atgagaaaac tgatagtatt cgcttttatt tattggggaa aaattggcag
    45421 aggcgtgtgg aaacacttgg tcgctcagac agctatgacc cagataaagg tgtcttatta
    45481 ttgtaaaaat ctcttgtgcg aagctagctt tcacagaaaa accttgcttg ctcgcgcaaa
    45541 aataacttaa aaaagaagcg aaatggagat aaaaaggctt aaaacacctc tatgtgtgcc
    45601 atccgtttct ctataaactg tgtcatttgg cgcagtctca cccttcatgg gtgagtggat
    45661 tgaaatatta ttttattaaa gttatcacgt aaattttgca agtctcaccc ttcatgggtg
    45721 agtggattga aatcattata tgaacaatgc ctttgcggaa ttagttgtct cacccttcat
    45781 gggtgagtgg attgaaagat gaacccgacc tcattaaaag agaggtcggg tttttacatg
    45841 cctttagtca accatttatc tgatagcgta tcaaattcaa gtttttgcat gttctagtct
    45901 atggtttgcg ccactagctt tcggtaaact aagggtaact taagagagga gttatttatg
    45961 atactatcac ttgtattaat ggtatcggtt tttggggcag gcttgttatc atttttctct
    46021 ccgtgtatct ttccggtcct tcctgtctat ttaggcattt tactggatgc agatgattca
    46081 aagacgatta ctatatttgg taaaaaactc tattggtatg gcattgtcaa aactttagcc
    46141 tttatttttg gtctatctac tatttttgtg attttaggtt acggggctgg ttttttagga
    46201 aatatccttt atgcggtatg gtttcggtac ttactgggag ccttggtcat tatcttgggg
    46261 attcatcaga tgggcctcat taccattaag agcttacaat ttcaaaaatc actgactttc
    46321 cataataaca agaatcgcaa cggtttgttc aatgcattta tccttggttt aacctttagc
    46381 tttggttgga cgccttgtgt gggacctgtt ttgagttctg tcctagcatt ggtggcttca
    46441 gggggaaatg gtgcctggca aggtggcgtt ttgatgatta tttatactct tggattgggc
    46501 attcctttcc tactcatctc ttttgcgtca ggcattgttt tgaaacagtt taacaagctc
    46561 aaaccccaca tacttttact gaaaaaagta ggaggcgttc tgattatagt catgggaatc
    46621 ttgcttatga caggaacctt aaataactta gcacaacttt ttggataaag gagaaacaca
    46681 atgaaaaaag gactattagt aacaactggt ttggcttgtc tcgggctact aactgcttgc
    46741 tcaacccaag acaatatggc taaaaaggaa ataactcagg acaagatgag catggcagct
    46801 aaaaagaaag ataagatgtc aacatcaaag gacaagtcca tgatggcaga taaatcatct
    46861 gataagaaaa tgaccaatga tggtcctatg gcgcctgatt tcgaactcaa aggcatcgat
    46921 ggtaagacct atcgcttatc agagttcaaa ggtaaaaaag tttatttgaa attttgggcc
    46981 tcttggtgtt caatctgtct atcaaccttg gcagataccg aagacctggc taaaatgtca
    47041 gataaagact atgtggtcct aacagttgtc tcaccaggtc atcaagggga aaaatcagaa
    47101 gcagacttta aaaaatggtt ccaaggaaca gactataagg acttaccagt cttgttagat
    47161 ccggatggca agctattgga agcttacggc gtcagatctt acccaacaga agttttcatt
    47221 ggaagtgatg gtgttcttgc taaaaaacac attggttatg ccaaaaaatc agacatcaaa
    47281 aagaccctta aaggtataca ttaggaagga ccagctgata ggcattagaa aaaaggagtt
    47341 gagtttatgg aacacaaatg gaaattatgg ctatttatcg ttggagtggt cttgttaata
    47401 ggcggagctt ctgttctcat tcagaaaggt caagccaagg cttttagtca aggcaagcct
    47461 caactaccag ctaaaaaagt caaatccact aaagtagatc ccaatgcccc tgttgatcaa
    47521 aaaatgattt acctagctgg tggctgtttc tggggcgttg aagaatattt ttcacaagtg
    47581 gatggcgtct tagatgcggt gtcaggatat gccaatggta gaggcgatac cactaattac
    47641 cagttaattc atcagactgg tcatgcagaa accgttgaag tggcctatga tgcgaaccgt
    47701 attagcttga aagaattatt actgcatttt ttccgaatta ttgacccaac cagtcttaat
    47761 aagcaaggaa atgatcgcgg tagtcaatac cgaactggga cttactatac tgacaaagca
    47821 gatttagcta ttattgatga ggtcttcaaa gaaaaagcta aggattacaa gaaaaaaata
    47881 gtggttgaaa aagcaccatt gaaacacttt attaaggcag aagactacca ccaagattac
    47941 ctcaagaaaa atccaaatgg ctactgccat attgatatta accaagcgac ttatcctgtg
    48001 attgatgaaa gcaagtaccc aaaaccaagt gccactgaga tcaaagcaaa actatctgca
    48061 gacgaatacc gagtgacgca aaaaaacgaa actgaaaaag ccttttcaaa ccgctactgg
    48121 gattcttttg atgcaggaat ttacgtagac gttgtgacag gagaaccgct tttctcttca
    48181 aaagacaaat ttgaatcagg ttgtggttgg ccaagtttta gccgccccat cagcccagat
    48241 gttattcgtt acaaggaaga taagagtttc aatatgacga gaacagaggt gcgcagccgt
    48301 tcaggggatt ctcatctggg gcatgtcttt actgatggac ctaaagacca aggtggcctt
    48361 cgttactgca tcaatagctt gtccattacc tttattccaa aagctgatat ggaagctaag
    48421 ggctatggtt atttattatc atctgtagaa tagggttagg cctaaaaatc tgatataata
    48481 gaagaaagac taatatgaga gggccatgca aggcccttta taaagataag gagacaccgt
    48541 gtgtactcat tgttaattgt agaagacgaa taccttgtgc gccaaggtat tcgttctttg
    48601 gttgatttta gccagttcaa gattgatcgg gtcaacgaag cagaaaatgg ccagttggct
    48661 tgggacttgt ttcagaaaga gccttatgat attgttttga cggatatcaa tatgcccaaa
    48721 ttaaatggga ttcaactagc agaactcatt aaacaggaat ccccccaatc tcatctggta
    48781 tttttgacgg gctacgatga ttttaactat gccttatctg ctttgaaatt aggggcagat
    48841 gattacttgc tcaaaccctt ttccaaggca gatgtagaag acatgttagg aaagctccgg
    48901 aaaaaattag aactttccaa gaaaacagaa accattcagg aattggttga gcagcctcaa
    48961 aaagaagtat cagcaatagc aatggctatt catgagcggt tggcagattc tgatttgacc
    49021 ctaaaaagct tggctcagca gcttggtttt agccctaatt accttagcgt cttgattaaa
    49081 aaagatttag ggatgccctt tcaagattat ctggtacaag aacggttgaa aaaagccaag
    49141 cttctcttgt taaccagtaa tcttaaaatc tatgaaattg cagagcaggt cggctttgag
    49201 gacatgaatt atttttctca gcgctttaag caactggtag gtgttacccc aagtcagtat
    49261 aaaaaaggag gtcaggcatg aagcgttatc ccctactggt ccagttgatt tcgtatgtct
    49321 ttgtgatcgt aattgccctt attaccacac tgggcttact ctattaccag accagttctc
    49381 gtaatatcag gcagttaatt gaacgtgata ctcggcagag cattcggcaa agttcccaat
    49441 ttattgacgc ttacatcaag cctcttaaag aaacaacttc ggtgctggcg aaaaatacgg
    49501 aaattcaagc ctttgctagt caaattcatc aagaaaatga caagcaggtc cttcagctca
    49561 tgaagatggt tcttgcgacc aattccgatt tacaagcagc tgttctggtg actaaggatg
    49621 gtcgaacggt gcctaccaat tctcagttga ccatgaaaac ctccagtgac atgatggcag
    49681 aaccctggta taaagcagcc atagaccgtc aggccatgcc agtcttgacc ccagctcggc
    49741 aattatccct ttcttctaaa aaagaatggg tagtttctgt gacccaagag gtagtggatg
    49801 ctgctggtca taatttaggc gtgctaagac ttgatattgc atacccgacc attaaagcgt
    49861 ctttagatca gcttcagcta ggccgccaag gctttgcctt tattgtgaat gataagcatg
    49921 aatttgttta ccatcccaaa aagagtgttt acagttcttc taaggagatg gctgcgatga
    49981 agccttattt agcgattcag aatggttaca ctaaggacaa gacatctttt gtttaccaaa
    50041 aactcattcc taacagtcaa tggactttag tgggagtggc gtcactggat cagttgcacc
    50101 gggtgcagcg ccaaattttt tggtcctttc ttggaacagg gcttctagcc tgtctgattt
    50161 gtggctttgc aactgtctta gccttacgca gatggattcg tcccattcaa caattgcagc
    50221 aagttattct tgctattcaa aaaggagatc gtcagttacg tgcccaagaa acgggttctc
    50281 cagaattgac agaccttgcc caacagttta atgcgctctt agatcaaatt gatagcttga
    50341 tggttgccgt tgcggataag gaaaaggcga ttgggcagta taggttacaa gccttggcta
    50401 gtcagattaa cccgcatttt ctctataaca ccttggacac tattatttgg atggcagaat
    50461 ttaatgacag caagcgcgtg gtagaagtga ccaagtctct agctaagtat tttcgtttgg
    50521 cccttaatca ggggaacgaa tacattcgtt tggcagatga actggatcac gttagccaat
    50581 acctctttat tcaaaaacag cgctatggag acaagctaag ttatgaagtg caaggcttag
    50641 atgtctacgc agactttgtt attcctaagc ttatcttaca gcccttagta gaaaatgcta
    50701 tctaccatgg catcaaagaa gtcgatcgca aaggcatgat caaggttacg gtatctgata
    50761 cagctcagca tctggtgttg actgtttggg ataatggtaa aggcattgaa gactcttcac
    50821 tgaccaatag tcagagcttg ttggctaggg gaggtgtggg ccttaaaaat gttgaccagc
    50881 ggttaaaact tcactatggt gaaggctacc acatgaccat tcatagccag tcagaccagt
    50941 tcactgaaat acaattaacc cttcctaaaa tgcatgaatt aatggcagac gacacacagg
    51001 aaaatgagta aggttacagg aagtgcatgt cagccatttc cctaagttag ggtattggag
    51061 ataagaacct tagaaaggat atcatgagtt tagcatctga ctattttagc cgacagaccc
    51121 ccatagttga aaagctaatg gcttacggtt ttgaaaaacg aaataatggt tacttctata
    51181 acgaacggtt tatggaagaa gaatttgagg cgcagcttag gattgatgag gctggcaata
    51241 tctgggatcg ggttattgat tgtgacttgg aggaggatta tttgccttta caacaagcag
    51301 cttggcaagg aacctatact gggcaggtta gagcggccta tttggaactt ttggagcgct
    51361 tatcagtagc ctgttttgaa gtcactcctt ttcagtcaat gcaagctaat agacttgcta
    51421 agcatatcac caaagaatgg tctgacccaa tggactatcc ttttgaaaag cacccagacc
    51481 ttgcaaccta tcgggtaggg ggcaaatggt atgctatgat tttttctctc ttggctgata
    51541 aattagacca gataccagag agattagtag gacagacatg tgaagtgatg actgtcaaag
    51601 ttaatcccaa ggatttacct cagttactgc agcaagaagg catttaccca gcgtatcaca
    51661 tgtcaaagaa aaactggatt agtattattt tggatgataa ggtaacagat gataagcttt
    51721 ggacgttagt gactcaaagt cgtcaactag tcaacccaaa cggtctttct aatcctaatg
    51781 gtcctgatta ttgggtcatt ccagctaatt tgaaatacta tgatattgat gcagaatttg
    51841 cagccaatga tgtgatttta tggacccaaa aagctggtat tgccgtggga gactacgtgc
    51901 tgatttatat gacagcccca gtcaaatcca ttcgctatgc ctgccaagtt ctagaaactg
    51961 atatacctaa cgagggttat cgtaaaaatc ctaacattga caaactcatg cgtctccgaa
    52021 aatgccaaca gtacaaggat ggtctcttgt cttttgacct tatgaaaaaa catggcgtag
    52081 ctgcagtgag agggccaaga cgattaagcc ctcaattaat tgcattttta aaggaaaaag
    52141 agtatcttaa agaaaacaac taaggggact aaaggagaat aagagatggt tgaatttgta
    52201 gcagatcaaa gtttttggga actttttcca gatgccaaac taggtgtcgt cttattaaag
    52261 gatgttcaga atcaagcaga gtcaccagaa gatgtcaaac agctcttagc agatagccat
    52321 gagttggtaa aagcttattt aacagcagac aattttagtg ataatcaggt ggttcaagtc
    52381 taccgcaagg cctatcaaca ctttaaaacc aaaaaaggag ctcgctctag cattgaagcc
    52441 cttctcaagc gggtgtcaaa tggtcaaagc attccgtcta ttaacccact agtggatatt
    52501 tataatgcgg ctagtctacg ttttggttta cctgttggag ccgaagatag tgacagtttt
    52561 atcggagatt tgcgtctgac gacaacagat ggtggtgacg acttttactt gattggggat
    52621 gctgacaaca acccaacctt gccaaatgag ctgtgttata aagatgatat tggagcggtt
    52681 tgtcgttgcc ttaactggcg agatggcgaa cgcaccatgg tgacagaaca caccaaaaat
    52741 gcatttctga ttatcgaagc tcttgatcaa gaaggtcaaa atagattgca agaagctctt
    52801 gacttcattg aaggcagcgc taaaatgtac cttcatgcaa tcacttctgt gcatgtgttg
    52861 gataaagaca atccacacgt gccacttttt acagaaaact agattagtag gatgagctgt
    52921 gtaaaataga aggttaacaa aaaagcgagt aaacgaatgt cctgtcattt aggttttcgt
    52981 ttactcgctt tttgatgaga ttttactgct gtcctagcaa aaggtgagga tgaggtcgta
    53041 tatcacatag aggttttctg ttaagcctta tttgttacta aagaagaaag gtggcgtgta
    53101 atgactaagg gtttcgtaac aagccttggc ttgctcattg tcttcacaga caatcaagca
    53161 agtatcatca ccacagaccg ttgctacaat ttctggaatt tgcatggcat ctaaaatgga
    53221 gccaaaggat tgggctagtc caggtaacgt ttttaaaata atttgatgtt gaactatctt
    53281 taacatgacc aaagcgtctt ccatgtaaaa acgaagacga tgttcccagc gggtttgtga
    53341 aatggctaac gcttcatagt gtgtgtcgtt tccagaagtt actttgacca ggttcaattc
    53401 cttcatgtca cgtgataggg tagcctgagt gatcgtgatg ccattttttt gcaaccgttc
    53461 ttgtaattct tgctgtgtat gtatagttgt ctcagagatg agggaacgta tgagttggtg
    53521 acgcgtctct ttcttattca ttgttataat cttcctttaa cgtaattgat aacaatccga
    53581 gtatagcaaa aagaggagaa ggtgtaaagt ttttgatgaa ccgcttacaa aatggctttt
    53641 ttatggtaaa ataaagatga tgtttagaaa aaggagattt gtgtgattcg aagagaagat
    53701 tatcaatacc ttagaaaact aaacgatttt cgctactttt ctattgaaca atttgacaaa
    53761 attgtagggc agatggaatt ccgaaaagct aaaaaggacc atatcctttt ttttgaggga
    53821 gataaaagag ataagctttt tctagtgacc tctggttatt tcaaggtgga gcagtccgat
    53881 caatcaggaa ccttcatgta tacggatttc atcagacacg gcaccatttt tccttacggt
    53941 ggcctcttta cagatgatta ttaccatttt tcagtggtag ccatgacaga cgtgacttat
    54001 ttttactttc cagtcgattt atttgaagag tattcgcttg aaaatcgtct acaaatgaaa
    54061 cacttatata gtaagatgtc taagctgctc gagttacatg agttgagggt ccgaaacttg
    54121 attacatcaa gtgctagttc tcgtgtgatt caatctctgg cgattttgct ggttgaaatg
    54181 ggtaaagaca gtgatacctt accttttcaa ttaacaacaa cagatattgc tcaaataagt
    54241 gggacgacaa gagaaacagt tagccatgtg cttagggatt tgaagaagca agaattgatt
    54301 accataaaag gtaagtattt gacttatcta gataaagact atttttt
//
LOCUS       DAUGHY010000006        52239 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.006, whole genome shotgun sequence.
ACCESSION   DAUGHY010000006 DAUGHY010000000
VERSION     DAUGHY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 52239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52239
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.006"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            137..1372
                     /gene="arcA"
                     /locus_tag="VH752_000333"
     CDS             137..1372
                     /gene="arcA"
                     /locus_tag="VH752_000333"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEQ8288710.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
     gene            1411..1764
                     /locus_tag="VH752_000334"
                     /pseudo
     CDS             1411..1764
                     /locus_tag="VH752_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1804..2817
                     /gene="argF"
                     /locus_tag="VH752_000335"
     CDS             1804..2817
                     /gene="argF"
                     /locus_tag="VH752_000335"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEQ8288711.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMDLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            2987..4480
                     /locus_tag="VH752_000336"
     CDS             2987..4480
                     /locus_tag="VH752_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEQ8288712.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDTGIASVVRKNKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVGFDS
                     IVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSIWFV
                     YNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMILSL
                     IPWEDFGIKFFTNINTWLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVY
                     RMSEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQ
                     VFVILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNM
                     ISPTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            4497..5828
                     /locus_tag="VH752_000337"
     CDS             4497..5828
                     /locus_tag="VH752_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEQ8288713.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFYTYIDPKGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTEPFDC
                     VEAGGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGSDKLSLEIGQAYNVVPARASYQG
                     DKLETLQKELDKLGFEYVVKEGELTVYGLAQHAKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEIRLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAVQTEHQENEHIVLADAYRAMDIYAEAIYRLTQ"
     gene            5848..6798
                     /gene="arcC"
                     /locus_tag="VH752_000338"
     CDS             5848..6798
                     /gene="arcC"
                     /locus_tag="VH752_000338"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEQ8288714.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALMSTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKSLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYINFNKPDQAKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
     gene            7112..8104
                     /locus_tag="VH752_000339"
     CDS             7112..8104
                     /locus_tag="VH752_000339"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEQ8288715.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLAF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKKHIGEVQTSVWPQEVRDSYDNIL"
     gene            8227..8766
                     /gene="rsmD"
                     /locus_tag="VH752_000340"
     CDS             8227..8766
                     /gene="rsmD"
                     /locus_tag="VH752_000340"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEQ8288716.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            8756..9247
                     /gene="coaD"
                     /locus_tag="VH752_000341"
     CDS             8756..9247
                     /gene="coaD"
                     /locus_tag="VH752_000341"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEQ8288717.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            9234..10271
                     /locus_tag="VH752_000342"
     CDS             9234..10271
                     /locus_tag="VH752_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEQ8288718.1"
                     /translation="MKRLNKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDADFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRAAKRLKTKMKIVPVT
                     TVQEALVYLRK"
     gene            10619..10699
                     /locus_tag="VH752_000343"
                     /pseudo
     CDS             10619..10699
                     /locus_tag="VH752_000343"
                     /inference="COORDINATES: protein motif:HMM:NF019884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10676..11652
                     /locus_tag="VH752_000344"
                     /pseudo
     CDS             10676..11652
                     /locus_tag="VH752_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
     gene            12076..12720
                     /locus_tag="VH752_000345"
     CDS             12076..12720
                     /locus_tag="VH752_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEQ8288719.1"
                     /translation="MDFSERCQMKKEISPEMYNYNKFPGPKFIHFEEQVKAEGIDLLL
                     LEDVKNAFDTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLE
                     DYIKEFCNFGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPATPK
                     SKSKRGSKEKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISHFIIRKKDK"
     gene            12724..13803
                     /gene="rlmN"
                     /locus_tag="VH752_000346"
     CDS             12724..13803
                     /gene="rlmN"
                     /locus_tag="VH752_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEQ8288720.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSRIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSLKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(13885..14511)
                     /locus_tag="VH752_000347"
     CDS             complement(13885..14511)
                     /locus_tag="VH752_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEQ8288721.1"
                     /translation="MIAFFSICSGILLTHLFYCLLLYFPDNGQEQLSKQTVIVCDQLT
                     FAKRVFRQFQNHFITSQKIFFSLEISLIIILWASMVHWVSASYCYLLLFSLLFSLFDW
                     RSQEYPFILWLFSFVSLLLFYSINYLSLILLLLGLLAHLRPFSIGAGDFFYLASLALV
                     LDLTSLIWLIQLASLAGITACLLLGIKRIPFIPYLSFGLFWIVLLEHF"
     gene            14739..15266
                     /locus_tag="VH752_000348"
     CDS             14739..15266
                     /locus_tag="VH752_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEQ8288722.1"
                     /translation="MTNTLVENIYASVTHNISKKEASKNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            15408..15611
                     /locus_tag="VH752_000349"
     CDS             15408..15611
                     /locus_tag="VH752_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEQ8288723.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLD
                     KRDYLNAELILRREHRLELEKEG"
     gene            15616..16587
                     /locus_tag="VH752_000350"
     CDS             15616..16587
                     /locus_tag="VH752_000350"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEQ8288724.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIVASIKHRLDLYGLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGIIADGNLIHGVAGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFADSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            16597..16986
                     /locus_tag="VH752_000351"
     CDS             16597..16986
                     /locus_tag="VH752_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEQ8288725.1"
                     /translation="MSPITLILWLLLVGIAGYYTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRAKHILGARNFPAQQFDAAIKGLRKDKPVLIYENMRPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            17168..19009
                     /gene="typA"
                     /locus_tag="VH752_000352"
     CDS             17168..19009
                     /gene="typA"
                     /locus_tag="VH752_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEQ8288726.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            19131..19382
                     /locus_tag="VH752_000353"
     CDS             19131..19382
                     /locus_tag="VH752_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEQ8288727.1"
                     /translation="MFYLIMAVLIISYYLYMAPKSVRNTLGMIGLVGLVALLIVLAGL
                     SFIKIMQIPPEFFIGMGMVALGYFALKDVRKMTKKPRVK"
     gene            19738..21096
                     /locus_tag="VH752_000354"
     CDS             19738..21096
                     /locus_tag="VH752_000354"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEQ8288728.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDENFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISATLAKTTKATVIPFSTQKVVDGAYLNDGILYFKEQAIIAATDLGVPG
                     SHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATKKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKA
                     EVSYLEARNVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRG
                     DA"
     gene            21096..22178
                     /locus_tag="VH752_000355"
     CDS             21096..22178
                     /locus_tag="VH752_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEQ8288729.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFIIAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDANQMPESTQLEAVKEYFSRDLKTLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISSAIKEK"
     gene            22179..23327
                     /locus_tag="VH752_000356"
     CDS             22179..23327
                     /locus_tag="VH752_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEQ8288730.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTSKPKKPKKVRQPKEKSATQIAFQK
                     SLPVLLGALLLMAVSIFMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLT
                     SPGQYERPILRTIPWVKSVHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRV
                     DKVRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLI
                     EMYDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTP
                     EQNAADKEGDKPGEHQEQTDNDSETPANQSSPQQAPPSPETVLEQAHG"
     gene            23539..24903
                     /gene="ftsA"
                     /locus_tag="VH752_000357"
     CDS             23539..24903
                     /gene="ftsA"
                     /locus_tag="VH752_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEQ8288731.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGIKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            24927..26246
                     /gene="ftsZ"
                     /locus_tag="VH752_000358"
     CDS             24927..26246
                     /gene="ftsZ"
                     /locus_tag="VH752_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEQ8288732.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNKRGNFAIEGIEELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITSPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERIVEAARKAIYSPLLETTIDGAQDVIVNVTGGLDMTLTEA
                     EEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVRQEKAEQVSGFRQPRTFT
                     QTNAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREIPSAQKVISNHNQNQGSA
                     FGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETPPFFKNR"
     gene            26249..26920
                     /locus_tag="VH752_000359"
     CDS             26249..26920
                     /locus_tag="VH752_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEQ8288733.1"
                     /translation="MDLLTNKKKIFETIRLSTEAANRTNDSVSVIAVTKYVDSTIAGQ
                     LIEAGIEHIAENRVDKFLEKYDALKCMPVKWHLIGTLQRRKVKEVINYVDYFHALDSV
                     RLALEINKRADHPVKCFLQVNISKEESKHGFNISEIDEAIEEIGKMEKIQLVGLMTMA
                     PANASKESIITIFRQANQLRKNLQLKKRKNMPFTELSMGMSNDYPIAIQEGSTFIRIG
                     RAFFH"
     gene            26932..27588
                     /locus_tag="VH752_000360"
     CDS             26932..27588
                     /locus_tag="VH752_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEQ8288734.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVAASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMHPKHGTSERYYQQSQPKEGHEMVDRRKRMS
                     TSGIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            27593..27847
                     /locus_tag="VH752_000361"
     CDS             27593..27847
                     /locus_tag="VH752_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEQ8288735.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            27847..28638
                     /locus_tag="VH752_000362"
     CDS             27847..28638
                     /locus_tag="VH752_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEQ8288736.1"
                     /translation="MVSHSKIYQHFHQEEYPFIDRMSDMINRVEDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNSQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRLA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            28648..29406
                     /locus_tag="VH752_000363"
     CDS             28648..29406
                     /locus_tag="VH752_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEQ8288737.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKAKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSTTEPEVNLSETQTFKLNI"
     gene            29679..32480
                     /locus_tag="VH752_000364"
     CDS             29679..32480
                     /locus_tag="VH752_000364"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEQ8288738.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQELN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRDDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLDSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMMENAGPDFHGQFYNKVTPIVIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDIRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPATDTVAYADLGAVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEYESEAFVQLAEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKIIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGVAFMVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESGK"
     gene            complement(32744..33046)
                     /locus_tag="VH752_000365"
     CDS             complement(32744..33046)
                     /locus_tag="VH752_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEQ8288739.1"
                     /translation="MRLINTTSSHPELIRNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(33097..33552)
                     /locus_tag="VH752_000366"
     CDS             complement(33097..33552)
                     /locus_tag="VH752_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ8288740.1"
                     /translation="MMIPTFGHKNAHKDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLGWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
     gene            complement(33680..35962)
                     /locus_tag="VH752_000367"
     CDS             complement(33680..35962)
                     /locus_tag="VH752_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ8288741.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPANSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTPNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQIAKYKEMQAQKVDEQDIPIITEKTIEAIIEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSADNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEESLD"
     gene            36260..36490
                     /locus_tag="VH752_000368"
     CDS             36260..36490
                     /locus_tag="VH752_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEQ8288742.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            36617..37303
                     /locus_tag="VH752_000369"
     CDS             36617..37303
                     /locus_tag="VH752_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ8288743.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVL
                     ERHMAQGGNH"
     gene            37303..38037
                     /locus_tag="VH752_000370"
     CDS             37303..38037
                     /locus_tag="VH752_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ8288744.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTILENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSRVL"
     gene            38186..39595
                     /locus_tag="VH752_000371"
     CDS             38186..39595
                     /locus_tag="VH752_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEQ8288745.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQHFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRVFNPVT
                     QSKRFDPKGEFIKAYLPQLEHVPEKYLHEPWKMPKYLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKAKIE"
     gene            39773..41467
                     /locus_tag="VH752_000372"
     CDS             39773..41467
                     /locus_tag="VH752_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ8288746.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYQEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVT
                     KQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATF
                     GYYNERQIALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQN
                     CLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     gene            41675..42529
                     /locus_tag="VH752_000373"
     CDS             41675..42529
                     /locus_tag="VH752_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEQ8288747.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKEGAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            42682..44022
                     /locus_tag="VH752_000374"
     CDS             42682..44022
                     /locus_tag="VH752_000374"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEQ8288748.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQPLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHALANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDTSRIIARGYAMIEKNQALVVSVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            44000..44215
                     /locus_tag="VH752_000375"
     CDS             44000..44215
                     /locus_tag="VH752_000375"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEQ8288749.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            44215..45087
                     /locus_tag="VH752_000376"
     CDS             44215..45087
                     /locus_tag="VH752_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEQ8288750.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASCQLLTESLDQALTIFQTLESDVGFKP
                     QIITKLIEGLRLNA"
     gene            45080..45907
                     /locus_tag="VH752_000377"
     CDS             45080..45907
                     /locus_tag="VH752_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEQ8288751.1"
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVFASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKELDFSPIQGGHGNIEFLAHLVKSQTPETLAPHLIQEVVAKAHKEFEKHEKK"
     gene            45894..46364
                     /locus_tag="VH752_000378"
     CDS             45894..46364
                     /locus_tag="VH752_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ8288752.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            46385..48046
                     /gene="recN"
                     /locus_tag="VH752_000379"
     CDS             46385..48046
                     /gene="recN"
                     /locus_tag="VH752_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEQ8288753.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEY
                     QLLTGDDLSSGDLEAELKSLEKQLVAAASELSVSRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            48218..49147
                     /locus_tag="VH752_000380"
     CDS             48218..49147
                     /locus_tag="VH752_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ8288754.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFNDDSFISQNETDKMFENLTDDR
                     LLDELDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPHLRSLSD
                     TALRSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVLAAHYEDR
                     KAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLGMAVAKRKIA
                     LPQTGEEFSYYPALLGLMILGLTPIMIPKKINN"
     gene            49363..50214
                     /locus_tag="VH752_000381"
     CDS             49363..50214
                     /locus_tag="VH752_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ8288755.1"
                     /translation="MEKYMGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSD
                     NDLKEEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTI
                     EASRQGAEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTE
                     LYIGVSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLD
                     SYLAKNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAF
                     AVMVRYE"
     gene            50207..51049
                     /locus_tag="VH752_000382"
     CDS             50207..51049
                     /locus_tag="VH752_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEQ8288756.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLTKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNIKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            51027..51614
                     /locus_tag="VH752_000383"
     CDS             51027..51614
                     /locus_tag="VH752_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEQ8288757.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            51713..51988
                     /locus_tag="VH752_000384"
     CDS             51713..51988
                     /locus_tag="VH752_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEQ8288758.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
BASE COUNT        16540 a         8984 c        11036 g        15679 t
ORIGIN      
        1 atgtaagcga ttactcaaaa taactgtgag aaatacccta tttttatctt ttttaaggag
       61 tacactagga atataagcaa gagttaagaa tcttgacggt gaattcacaa aaaccaatta
      121 gaaagaggta attcttatga ctgctcaaac accaattcat gtttattctg aaattggaaa
      181 attaaaaaaa gtgttgctcc acagaccagg taaagaaatt gaaaatctta tgccagacta
      241 cttggaacgc ttgttgtttg atgacattcc attcttagag gatgcccaaa aagaacatga
      301 tgcttttgcc caagctttgc gtgacgaagg tattgaagtc ttgtaccttg agaccttagc
      361 tgcagaatct cttgtcactc cagaaattcg cgaagctttc attgatgaat acctaagtga
      421 agctaatatc cgtggaagag ctactaaaaa agccattcgt gagttgttga tggctatcga
      481 agacaatcaa gaattgattg aaaaaaccat ggcaggggtt caaaaatctg aacttccaga
      541 gatcccagca tctgaaaaag gcttgactga cttggttgaa tccaattacc catttgccat
      601 cgacccaatg ccaaaccttt atttcacacg ggacccattc gcaactattg gtacaggtgt
      661 ttctctcaac cacatgttct cagaaacccg taaccgtgaa accctttatg gtaaatacat
      721 tttcactcat cacccaattt atggtggtgg caaagtgcct atggtttatg accgtaatga
      781 aaccactcgc attgaaggtg gggacgaact tgttctttca aaagatgtgc ttgcggttgg
      841 tatttctcaa cgtacagatg ctgcttctat tgaaaaattg ttggttaaca tctttaaaca
      901 aaaccttggc ttcaagaaag tattggcctt tgaatttgca aataaccgta aattcatgca
      961 cttagacact gtctttacca tggttgacta tgacaaattc accattcacc cagaaattga
     1021 aggcgatctt cgcgtttact ctgtgactta tgacaacgaa gaacttcata ttgttgaaga
     1081 aaaaggtgat ttagcagaac ttcttgctgc taaccttggt gttgaaaaag ttgaccttat
     1141 ccgttgtggt ggtgacaact tagtagcagc aggtcgtgaa caatggaacg atggttctaa
     1201 cacccttact atcgcaccag gtgtggttgt ggtttataac cgtaacacca ttaccaatgc
     1261 cattcttgaa tctaaaggct tgaaattgat caagattcac ggaagtgaat tggttcgcgg
     1321 tcgtggtgga cctcgttgta tgtcaatgcc atttgaacgt gaagatattt aataagctat
     1381 ggtaaaggtg gttataggtc agaagccttt tcaacaggac aatttttagc tgaaacgaaa
     1441 atagaagcac gattgacacg cattgtaacc ttagcagatt attgtggttg cggttatggt
     1501 gccaaagtca ctgaagcgct agaaacttat accagacgag aaggctttta ccaactaacc
     1561 attcacagtg aactgacagc acaaaccttt tatgaaaact taggttatca gacctatggt
     1621 cccaagtgtt tagaagatgg tgagtattgt cagtcccttg caaaaaccat tcttaaatgg
     1681 gagaagaata tggacatagc aatgctaatt gcgattgttg gtggtctatt aggctgctat
     1741 ctctatctca caaaaaataa ttaatcaaaa cagattacag ttaacactcg aaggagacaa
     1801 tagatgacac aagtatttca aggacgtagc ttcttagcag aaaaagattt tacacgcgct
     1861 gaattagaat accttattga tttttcagca catttgaaag atttgaaaaa acgtggtgtg
     1921 cctcatcact acttagaagg taaaaacatt gccctcttgt ttgaaaaaac atcaactcgt
     1981 acgcgtgcag cttttacaac agcagccatt gacctaggtg ctcacccaga atacctcggt
     2041 gccaatgaca tccaacttgg taaaaaagaa tcaacagaag acactgctaa agtattgggt
     2101 cgtatgtttg atgggattga attccgtggt ttcagccaac gcatggttga agaattggca
     2161 gaattctcag gtgtgccagt ttggaatggt ttgactgatg aatggcaccc aacccaaatg
     2221 cttgctgact atttaacagt gaaagaaaac tttggcaaac ttgaaggcct tacccttgtt
     2281 tactgtggtg atgggcgtaa taacgttgcc aattcccttc ttgtgactgg tgctatcctt
     2341 ggtgttaatg ttcatatctt ctcaccaaaa gaactcttcc cagaagaaga gattgtgaca
     2401 ttggctgaag gctatgctaa agaaagtggc gctcgtatcc ttatcacaga agatgctgac
     2461 gaagctgtta agggcgcaga cgtcctttac acagacgttt gggtatcaat gggtgaagaa
     2521 gacaaattca aagaacgtgt tgaattgctt caaccatatc aagtcaacat ggaccttgtt
     2581 caaaaagcag gaaatgacaa gcttatcttc ttgcactgct tgccagcttt ccatgacaca
     2641 aataccgttt atggtaaaga cgttgctgaa aaattcggcg tgaaagaaat ggaagtcact
     2701 gatgaagtct tccgcagcaa atacgctcgt cattttgacc aagctgaaaa ccgtatgcac
     2761 actatcaaag cggttatggc agcaaccctt ggtaacctct ttattccaaa agtgtaataa
     2821 ggactggtac tccttaactc atttcacata tcaatgtgac ccctttaatt taacaattta
     2881 actacttaaa aacgtacttt aactacatga caagtcgttc tggttttcgc gtccttgacc
     2941 ggaactagaa cggcttattt ttatgaaaag gtaagaggta atcattatga cagaagaaaa
     3001 aaaacggggt tttagaattc cttcttctta caccgttctt tttatcatta tagccatcat
     3061 ggcagtgtta acttggttta ttccagctgg cgcctacgaa accgctaagg gcggtggtgt
     3121 tatttcagga acctacaaaa cagtagcatc caatcctcaa ggattttttg acatcttgat
     3181 ggctcccgtt cgaggcatgt taggtgttga aggcactgat ggtgctattc aagtgtcttt
     3241 ctttatctta atggttgggg gctttttagg agttgtcaac aaaacaggtg ctcttgacac
     3301 agggattgcg tcagtggttc gtaaaaacaa aggtagagaa aaaatgttga ttgctatttt
     3361 aatccctttg tttgctctag gtggaacgac ctatggtatg ggggaagaaa ccatggcctt
     3421 ttacccactt ttaattcctg ttatgattgc ggttggtttt gacagtattg ttgctgtagc
     3481 cattattttg attggttctc aaattgggtg tctagcctcc actattaacc catttgcaac
     3541 tggtgtagca gctgatgctg ctggtgtcag cattgcagat ggaatgattt ggcgtgttat
     3601 ccaatgggtt attcttgtcg gcatgtctat ttggtttgtt tacaattacg ctagtaagat
     3661 tgaagaagac ccaagtaaat cattggttgc agacaaggaa gaagagcata aggaactctt
     3721 ccagttgcaa aattctggag aagacttaaa caagcgccaa cgaaacgttt tgaccatttt
     3781 tactttgacc tttgtcatta tgattcttag tttgattccg tgggaagatt ttggcatcaa
     3841 attctttact aatatcaata cttggttaac cacaatgcct atcttagggg gagttattgg
     3901 gaaaaccatg ggagcatttg gtacttggta tttcccagaa atcaccatgc tctttatcat
     3961 gatgggtgtc ttagttgcta ttgtttatcg tatgagtgaa gaagactttt ttagctcttt
     4021 tttgactggt gcaggtgaat ttttaggtgt tgccatgatc tgtgctattg cacgcggtat
     4081 tcaagttatc atgaatggtg gtatgattac agccactatc ttacacttag gtgaaacaag
     4141 tctttctggt ttatcttctc aagtttttgt gatattagct tatattttct acctcccaat
     4201 gtccttcttg attccatcaa catcaggact tgctggggct acaatgggga ttatggcacc
     4261 gcttggacaa ttctcaaatg tccctgctca ccttgttatt acagccttcc agtcagcttc
     4321 tggaatctta aacatgattt ctccaacttc agcaatcgtt atgggagcac ttgcgcttgg
     4381 tcgcgttgac cttggtactt ggtggaaatt cattggtaaa tttattgtaa tggtgatgct
     4441 tgtaagcgtg ctattacttg tagttgcaac attcttttaa cagataggag gttcctatgg
     4501 aatcctatat cacacctaaa catcaagagg cctgtgtagc tgccattaaa cagatcgttt
     4561 cttacccttc tgtttgtcat gaaggagaaa atggcacacc ttttggacag gccattcaag
     4621 atgttctcga agcaacctta gacttatgtc aaggtcttgg cttctatact tatattgatc
     4681 ctaaaggtta ctatggctat gctgagttag gtgaccaaaa agaggtcttg gctattctgt
     4741 gccatttgga cgttgttcca gaaggagatc gtcagttatg gaagacagag ccttttgact
     4801 gtgttgaggc aggcggctgt ttatttggtc gaggcactca agacgataaa gggcctacta
     4861 tgatggcact atttgccacc aaagccttga tagatgcagg cgttaccttt aataaacgca
     4921 ttcgttttat tttcggaaca gatgaagaaa ctttgtggcg gtgtatgaat cgctacaatg
     4981 atgtagaaga acaggctacc tttgggtttg ctcctgattc aagtttccct ttaacctatg
     5041 ccgaaaaagg cctacttcag gcaaaattag tggggaaagg ttctgataaa ctgtcacttg
     5101 aaatcggaca agcctacaat gttgttcctg caagagcctc ttatcaaggt gataagcttg
     5161 agactttaca aaaagaacta gataagcttg gctttgagta cgtcgtcaaa gagggagaat
     5221 taacggttta tggtctcgcc caacatgcta aagatgctcc cgatggcatc aacgctatga
     5281 ttaggcttgc caaagcttta gtggtccttg aacctaacaa aacccttgat tttcttgcta
     5341 atgtcattga tgaagatggc agagcactta atatttttgg cagaatcgaa gatgaacctt
     5401 caggcaaatt aagctttaat gccgcagggc ttaccttaac caaagacaag gcagaaattc
     5461 gcctagatat tcggattcca gttttggcag ataaggaaaa attagtccaa caattaagcc
     5521 aaaaagcaca agaatacggc ctaacctatg aagaatttga ttatttggcc ccattatatg
     5581 tgccattaga cagtgaattg gtaacaacct tgttatctgt ctatcggaaa aaaacaggtg
     5641 atcaaagccc tgcccaatca tcaggaggag caacctttgc tagaacaatg aagaattgtg
     5701 ttgcctttgg tgcccttttc cctgatgctg ttcaaacgga acaccaagaa aatgaacata
     5761 ttgttttagc ggatgcttac cgcgctatgg atatttatgc ggaagctatt taccgactaa
     5821 cgcaataaca agatatgagg agtaattatg acgaaacaaa aaatcgtagt cgcacttgga
     5881 ggaaatgcta ttctttcaac agatgcgtca gcaaaagcac aacaagaagc cttgatgtcc
     5941 acatcaaaat cattggttaa attaatcaaa gaaggtcatg aagtcatcgt gacccacggc
     6001 aatggcccac aagttggtaa tctcttatta caacaagcag cggcagattc tgaaaagaat
     6061 ccggctatgc ctttggatac ttgtgttgct atgacagaag gtagcatcgg tttttggtta
     6121 gttaatgccc ttgataatga attgcaagcc caaggtattc aaaaagaagt ggcagctgtt
     6181 gtcactcaag tgatcgtgga tgccaaagac ccagcttttg aaaacccaac aaaaccaatt
     6241 ggtccatttc ttaccgaaga agatgccaaa aaacaaatgg cggagtctgg tgcaagtttc
     6301 aaagaagatg caggtcgtgg ctggcgtaag gttgtgccat caccaaaacc agttggtatt
     6361 aaagaagcca atgtcatcag aagtctcgtt gattctggcg ttgttgtggt tagtgctggt
     6421 ggtggcggtg tgcctgttgt ggaagatgca actagcaagt ccctgactgg tgtggaagct
     6481 gttattgaca aggactttgc tagccaaacc ctttcagaat tggtggatgc agacctcttt
     6541 atcgttttga caggagttga caatgtttac atcaacttca acaaacctga ccaagccaaa
     6601 ttggaagaag tgactgtctc tcaaatgaaa gaatacatca cacaagacca atttgcacca
     6661 ggtagtatgt taccaaaagt ggaagctgcc attgcttttg ttgaaaacaa accaaatgct
     6721 aaagccatta tcacatcgct cgaaaatatt gacaatgtac tatcagcaaa cgctggaaca
     6781 caaattatcg cagggtaatc aagaggattt ttagttctgt ttaaaagact catttaatgg
     6841 tattttctga agagtacgac tgacataaag agttgatact gtttttaaag gaaaagagtg
     6901 tgccataata aaaaacaata atagtattag agaagactaa ttttattgag aattagcctt
     6961 ttcttttggc tttgataggt gtataataac catactgttt ttttattttt caaaatatta
     7021 gaataaacaa aagtggacaa cgtataaaga gaactgctaa gtcgttgagt tcattagtcc
     7081 caaaaagatt accaaaaatg aaggaggagc tatgaagaaa agttttattc atcaacaaga
     7141 ggagatttcc tttgttaaga atacgttcac tcagtattta attgctaagt tagatgttgt
     7201 tgaagtgcaa ggacccattt taagtcgggt cggggatggt atgcaagata atttgtccgg
     7261 tactgaaaat ccagtatctg tcaatgtctt gaaaattcct aatgcaacct ttgaagttgt
     7321 gcattcatta gcaaaatgga aacgtcacac cttagcccgt tttgggttta atgaaggtga
     7381 aggtcttgtt gttaatatga aggctctccg acctgatgag gattcacttg accaaaccca
     7441 ttctgtctat gttgaccaat gggactggga aaaagtcatc cctgatggca aacgtaatct
     7501 cgcttacctt aaagaaactg ttgaaaccat ttacaaggtc attcggctaa ctgaattagc
     7561 tgtagaagct cgttatgata ttgaggctgt tttacctaag aaaataacct tcattcatac
     7621 cgaagaattg gtagctaaat accctgattt gacacccaaa gaacgtgaaa atgccattac
     7681 taaagaattt ggagcagtct ttttaattgg tattggcggg gttttacctg acggtaaacc
     7741 acacgatggc cgcgctcctg actatgatga ctggacaaca gaaactgaaa atggctatca
     7801 tgggcttaat ggtgatattt tggtttggaa tgaccaactt ggttcagcct ttgaattatc
     7861 ctcaatgggg atccgtgtgg atgaagaggc cctaaaacgt caggtggaaa tgactggtga
     7921 ccaagaccgc ttagcctttg attggcacaa atctttgttg aatggacttt tccctctaac
     7981 gattggtgga ggaatcggtc aatcacgtat ggtcatgttt ttgttgcgta aaaaacatat
     8041 tggagaagtt cagacctccg tttggcccca agaagttcgt gatagctatg ataatatttt
     8101 ataagtaatc acatcatttt gctaagtttt ccacttgtat aagagacttt aaataaatga
     8161 tcagaaatat tgaaaaaaga aatcattttt aaggtttaga gctagaaatt tgctataata
     8221 aatgttatga gagttgtatc aggtgaattt ggtgggcgtc ctttaaagac gctcgatgga
     8281 aagataacac gccctacttc agacaaagtt agaggtgcga tttttaatat gataggacct
     8341 tactttaatg gtggtcgtgt tttagatttg tttgcaggtt caggtggatt agccattgag
     8401 gctgtttctc gtgggatgtc tgctgcagtt ttggttgaga agaatcgtaa agcgcaagct
     8461 attattcaag acaatattat aatgactaaa gctgagaatc ggtttacctt gttaaaaatg
     8521 gaagctgagc gagctattga ttgcttgact ggtcgatttg acttagtctt tttagaccct
     8581 ccttatgcca aagaaacaat tgtggcaact attgaagctt tagcagctaa aaacttattg
     8641 agtgaacagg ttatggttgt ctgtgagaca gataaaacag tcttgttgcc aaaagagatt
     8701 gcaacgttag gtatttggaa agaaaaaatt tatggaatta gtaaggtaac agtttatgtt
     8761 aactaaaata ggactttata cagggtcatt tgatcctgtt acaaacggtc atctcgatat
     8821 tgttaaacgg gcaagtggcc tatttgatca aatatatgtt gggatttttg ataatcctac
     8881 caaaaaaagc tattttaaac tagaagttcg taaagcaatg cttactcaag ctctggctga
     8941 ttttacaaat gtaattgtag taacttcgca cgaacgttta gctattgatg tagctaaaga
     9001 attacgagta actcacttga ttagaggctt gcgaaatgca actgactttg agtatgaaga
     9061 aaatttggaa tattttaatc accttttagc tccaaatatt gagaccgtct atttgatatc
     9121 aaggaataaa tggcaggccc ttagctctag tcgtgtgaga gagctaattc attttcagtc
     9181 ttccttagaa ggtttggttc ctcagtcagt tattgctcaa gtggagaaaa tgaatgaaaa
     9241 gacttaacaa aatcaaatgg tggttagtgg gtctgctagc tttaatctct ttgttgctag
     9301 cgttattttt tccgctacct tattatattg aaatgcctgg aggcgcttac gatattcgga
     9361 ctgtcttaca agtcaatggc aaagaagaca aacgaaaagg agcttaccag tttgttgcag
     9421 tgggcattag tcgtgccagc ctcgctcagc tattatatgc ttggctgaca ccgtttactg
     9481 aaattagtac agcagaagat acaacaggcg gatacagcga tgctgatttc cttcgaatta
     9541 atcaatttta catggaaaca tcacaaaatg cagctattta tcaagcttta tccttagctg
     9601 gaaaaccagt tacattagat tataaaggcg tatatgtttt agacgtaaac aacgaatcta
     9661 cttttaaagg aacgttacac ttagcagata ctgtaacagg tgtaaatggt aaacagttta
     9721 ctagttcagc agaacttatt gactatgttt ctcacctaaa actaggggat gaagttacgg
     9781 ttcagtttac gagtgataat aagcctaaaa aaggagttgg ccgtattatc aaactgaaaa
     9841 atgggaaaaa tgggattggc attgccttga ctgatcatac aagtgtcaat tcagaagaca
     9901 cagtgatctt tagtactaaa ggagtaggag gacctagtgc tggtctaatg tttactcttg
     9961 atatatatga tcaaataact aaagaagatt tacgcaaggg ccgtacaatt gcaggtacag
    10021 gaactattgg caaggatggc gaagtaggag atattggtgg tgcaggtctt aaagtagttg
    10081 cagcagctga agctggtgca gatatatttt ttgttccgaa taatcctgtt gataaggaaa
    10141 ttaaaaaagt taatccaaat gctataagta attacgaaga agccaaacgg gcagccaaac
    10201 gactaaagac caaaatgaag attgttcctg ttacgactgt tcaagaggca ctggtttatc
    10261 ttcgcaaata gaataactat gaatgtatta tattcacgct attaactcat aaaaaaacgg
    10321 ttgaaccaga tagacagatc cttgaccagt cttttgggtt caatcatttt ttacataaaa
    10381 catatctaac ttgacagcgt aaaaaagatg ttataaaagt ctagtggtaa aaaggcaata
    10441 gagaattgtg taaactttag tcgattatct ggaaatagag catactaaaa acacctagtg
    10501 aaaattgatt tttttcacta gctcatccaa gaactttcgc agataatcat gagtccattc
    10561 catgacttta ttattctgga aagccttgca atagacaagt taacgttata acaactaaat
    10621 taaaaacttg agattgaaaa atccccataa acttatttta caaggaggta aggctatggt
    10681 aaccattaaa caagttgctg aagaagcagg tgtttctaga tcaacagtct ccaggtatat
    10741 ttctcaaaaa ggatatgtat ctgatgatgc aaggcataaa atcaaagcag cgatagcaaa
    10801 attacattac actccaaatg tcttggcgca gtcattgaag acgaaaaaaa tcaactagtc
    10861 ggtttattat taccagacat ttccaatcct ttttttccga ggttagcaag gggagcagaa
    10921 gaatatttaa aagaaaaggg gtatcgggtt atgctaggaa atatttcaga cagcgaagct
    10981 cttgaagaag aatacgtcca tgtccttctt caaagcaatg ctgctggcat tattactacc
    11041 catgacttta caaagcggta tcctactctt gctattccag ttgtagtggt agatcgtgtt
    11101 gaccaagaga cccagtacgg tgtcttttca gataataggg cagggggctt attggcggct
    11161 cagactgtct ggcaagcagg ggcgaaagaa gttctgttaa taagaggtcc gctggataat
    11221 gctgaaaaca ttaatgagcg gtttgaagct agcttttctt accttcaaaa acaagacgtg
    11281 actatgtatg tgtgtgatag tcagaatttt gattttgaaa atattcagct agaagctagt
    11341 tccaacctca agtgttaccc aacaatagac agtattatag caccttctga tatccatgct
    11401 attgcttata ttcatgagct gcattctcaa ggtaaaaaga ttcctcaaga tgttcaaata
    11461 ataggttatg atgacatttt aatgagtcaa tttatttatc cttctttatc gactattcat
    11521 caatcctctt atttgatggg ccgttatgcc gccgaattag tttatactat tgctagtcaa
    11581 ttaactgtga aagcaaatcg gattaaattg cctgttcact acgttgagcg aaaaacaata
    11641 aggaaaagat aagtaataat attagtccag attttagcag gtatgatgaa gatttattgg
    11701 gatttacctt ctactttaaa agatgttatc tgtccttact ttcatcgcta tcagtcaaaa
    11761 cgcattctta tgcaactctt aaccttaagc ttaaggtctt tttgataaca tagaggaatg
    11821 ttacacgatg atgacgcaat aaagcctaaa tatagtaatt gtcgtgattt tatagctagt
    11881 gagtaatgag atagcaagtc tctcatttac ttacaaaagt caaaaaatag tgtataatgg
    11941 ttgagttaca gataacactg caaaaaagat aacaagtgtc cataattatc taagactata
    12001 aaggcgatat agacagatat aaaaaagatg acgaatcagt tatcactgga caattaaccg
    12061 atttagttac aactaatgga tttttcagaa aggtgtcaaa tgaaaaaaga aatttcgcca
    12121 gaaatgtaca actataataa atttccaggt cccaaattta ttcattttga agagcaagtt
    12181 aaggctgaag gcattgattt gttactctta gaagatgtta agaacgcttt tgatacgact
    12241 agctttggtc aacgttatac agaagttctc ctaaagtatg actatattgt tggcgattgg
    12301 ggaaatgaac agcttcgtct taaagggttt tataaggata gtgacgatat caagaaaacg
    12361 aatcgcatct cacgtttaga agattatatt aaagaatttt gcaattttgg ttgtgcttat
    12421 tttgttttag aaaatcttca tccacaagat attaaatttg aagaggagcg ccaaccaaga
    12481 cgaaagaaat cacctaaatc aaaatcaaat cgtcgcaagc caaactattc aaatcagcag
    12541 cccgcaacac ctaagagtaa atcgaagcgg ggatcaaaag aaaagcaacc tgaaaaccaa
    12601 gcgtttacca gtcaaaaacg tcgaagtaat actaagcata aagaaaagtc aaaacgtaat
    12661 cagactagtc aacttaatac caaaattagt cattttatca tcagaaagaa agataaataa
    12721 tccatgaaac catcgattta tagcttaaca cgcgatgaac tgatcgcgtg ggcagttgaa
    12781 cgcggccaaa aacagtttag agctactcaa atttgggatt ggctctataa aaaaagagtt
    12841 caatcttttg aggagatgac gaatatttct aaagattttg tttctatttt aaatgatagt
    12901 ttctgtgtca atcctttaaa acagcgagtt gtacaagaat cagctgatgg tacagtaaaa
    12961 tatttatttg aactgcccga tggtatgtta attgaaacag ttttaatgcg tcaacattat
    13021 ggtcattctg tttgtgtgac gactcaagtt ggctgtaaca ttggctgtac tttttgtgct
    13081 agtggattga tcaaaaagca acgtgatcta aacagtgggg aaattacagc tcaaatcatg
    13141 ttagtccaaa aatattttga cgatagaaag caaggtgagc gggttagcca tgttgtcgta
    13201 atgggcattg gtgaaccttt tgataattac aaaaatgtca tgtgctttct tcgcgtcatc
    13261 aacgatgata atggcttagc aattggtgcc cgtcatatta cagtctctac ctcagggtta
    13321 gctcacaaga ttcgggattt tgcaaacgag ggagtacagg ttaatttagc agtctcgttg
    13381 catgcaccaa ataatgattt acgttcaagg attatgcgag taaatcgctc ttttccgctt
    13441 gaaaagctct tttcagctat agaatactat attgaaaaaa cgaatcgtcg tgtcactttt
    13501 gaatacatta tgctaaatga agttaacgat agcattaagc aagctcaaga attagcagac
    13561 ttaactaaaa ctattcgtaa gttatcttat gttaacttga ttccgtataa tcctgtttca
    13621 gaacatgatc agtatagtag aagtcttaaa gagcgagttc tagcgtttta tgatgtgcta
    13681 aagaaaaatg gagttaactg tgtggtgcgt caagagcacg gaacagatat tgatgcagcc
    13741 tgtggccagt tacgttctaa aacgatgaaa aaagatcgcg aaaaggttac agcgacaaaa
    13801 taaagcccaa aaaatatctt tgattgacaa tgcttgatga caaaatattc ctagtcacta
    13861 aaatgactag gttttttatc tggcctaaaa atgctctaga agcacaatcc aaaataatcc
    13921 aaaactaaga taaggaataa aaggtatgcg cttgatgcct aatagtaagc aagcagtaat
    13981 acctgctaaa ctagcaagtt gaattagcca aatcaacgac gttagatcca atactaaagc
    14041 taagctagca aggtagaaaa aatcgcctgc tccaattgaa aaaggtctta aatgagctaa
    14101 taaacctagt agcagtaata tcaatgataa ataattgatc gaataaaaca ggagtagact
    14161 tacaaagcta aaaagccaca agataaaagg gtattcttga gaacgccaat caaataaact
    14221 aaataataaa gaaaagagta acaagtaaca gtaactagca ctaacccaat gtaccatact
    14281 agcccataag ataattatta gacttatctc taagctaaag aagatttttt ggctagttat
    14341 aaaatggttt tggaactgcc gaaaaacgcg tttggcaaaa gttagttggt cacaaacaat
    14401 gacagtctgt ttacttagct gttcttgacc attatcagga aaataaagta gtaaacagta
    14461 aaaaagatgg gtcaataaga tgcccgaaca tatactaaaa aaagctatca tacttattta
    14521 ttcgaaaaga aatcaaatac ttgcaatctt tccttaatca ttgtagaata tagatataaa
    14581 acgataatga ttctaattaa gtgtctaatc atccaattag gattattccg ttattgtcga
    14641 ataggattag gtccttaacc ctttcttatg tttatcacgt tagggttagt tccccctcca
    14701 ttcttgccct ttaagctgaa aagtaaggag atgttattat gacaaacaca ctcgttgaaa
    14761 atatttatgc ttcagtaact cacaatattt ctaaaaaaga agcatcaaaa aatgaaaaaa
    14821 ctaaagctgt tttaaaccaa gctgtcgctg acttatctgt ggctgcatct attgtgcacc
    14881 aagtacactg gtatatgcgt ggtccaggtt tcttgtacct tcacccaaaa atggatgaat
    14941 tacttgatag cttgaatgct aatttagatg aaatgagcga gcgtttgatc actattggcg
    15001 gagcgcctta ttctacccta gctgagtttt ctaaacactc taaattggac gaagcaaaag
    15061 gtacttacga taaaacagtt gctcagcatt tagcaagatt ggtcgaagtt tacctttacc
    15121 tcagcagcct ttaccaagtt ggtttggata ttaccgatga agaaggcgat gctggaacaa
    15181 atgatttgtt tacagcagct aagacagagg ctgaaaaaac catttggatg ctccaagcag
    15241 agcgtggaca aggcccagca ctctaattga ttatgcctta ggataaaaga agtcaatgat
    15301 aaaattggct tcttttttat ggaatcatcg attcattttc agaaacatag aaagcgattc
    15361 catacttgtc ttgaaatttg ccgtagaatt tgctaaaatc tacttatatg aaaacactat
    15421 atgatgtgca acaattgcta aaaaactttg gcatttttgt ttaccttggc aaacgtctct
    15481 atgatattga aatgatgaag attgaacttc agcgtctcta tgatagcggc ttattggata
    15541 agagggatta cttaaatgcg gagttaattt tgcgacgtga gcatagatta gaattggaaa
    15601 aagaaggata gacaaatgag tcaaaaatta ctggggattg atttaggtgg taccaccatt
    15661 aaatttggaa ttttaacagc agcaggagaa gtccaagaaa aatgggcaat tgagactaat
    15721 attttagaag gaggcaagca tattgttccc gatattgtag cttccatcaa acatcgctta
    15781 gacttgtatg gtctaagcag tgctgatttt gtgggaattg gtatgggatc accaggagcg
    15841 gttgaccgtg atactaatac agttactggg gcattcaacc ttaattggaa agaaacccaa
    15901 gaagtcggtt ccgttgttga aaaagaattg ggcattcctt ttgccattga caatgatgct
    15961 aatgtggctg cccttggtga acgttgggta ggtgctggtg aaaataaccc agatgtcgtc
    16021 ttcatgacac ttggaacagg tgtcggtgga ggcattattg ctgatggtaa cttgattcat
    16081 ggtgttgcag gagcaggtgg tgaaatcggc cacatgattg ttgagccaga aaatggcttt
    16141 gcttgtactt gtggctcaca cggctgtttg gaaacagtag cttcagcaac aggagttgtc
    16201 aaagtggcac gtttattggc agaagcttat gaaggggatt cagccatcaa agcagctatt
    16261 gacaatggtg aaggtgttac cagcaaagat atttttatgg cagctgaagc aggggattcc
    16321 tttgctgatt ctgttgtgga aaaggttggt tactaccttg gccttgcttc agcaaatatt
    16381 tccaatatat tgaatccaga ctcagtggtt atcggtgggg gtgtgtcagc agcaggagaa
    16441 tttctacgct cacgtattga aaaatacttt gtgaccttca ctttcccaca agtgaggtat
    16501 tcaactaaaa ttaagattgc agagcttggc aatgatgctg gaattatcgg tgcagctagc
    16561 cttgctcgac aatttatcac aaaataggag ggggatatgt ctccaatcac acttatctta
    16621 tggttacttc ttgtcggcat tgctggttac tacacatgga actatttctc ttttcgaaaa
    16681 atggcaaagc aggttgataa cgagactttt aaagacgtga tgcgtcaagg tcaattgatt
    16741 gatttacgag agccagcggc ttttagagct aagcatattt taggagctcg aaattttcca
    16801 gcgcaacaat ttgatgctgc tattaaggga ctacgcaagg acaaacctgt tcttatttac
    16861 gaaaatatgc gtccccagta ccgtgtgcct gcggtcaaaa aacttaaaaa agctggcttt
    16921 gaagacgttt atgtcttaaa agacggtatt gattattggg atggcaaagt taaacaaaca
    16981 acctagttaa gagtaacgtg gagttaaggg gaataaaggc agtcactgtc tcaaaaacct
    17041 taattccttt tttgctgtat ccagacttgc tgaaagtctg aaaacattta caattgatta
    17101 aaaccagttt tttaaaacat tttgtgttat acttatctag ttaaaatata tttacttaga
    17161 ggaacaaatg actaacttaa gaaacgatat ccgtaacgta gcgattattg cccacgttga
    17221 ccacggaaaa acaacacttg tagatgaatt attaaaacaa tcccatactc ttgatgagcg
    17281 taaagagctt caagagcgtg ccatggattc caatgacctt gaaaaagaac gtgggattac
    17341 gatccttgcg aaaaatacgg cagtagccta taacgatgtt cgtattaaca tcatggatac
    17401 cccaggacac gcggacttcg gtggtgaagt tgaacgtatc atgaaaatgg ttgacggggt
    17461 tgttcttgtt gtggatgcct acgaaggaac aatgccccag acgcgtttcg tattgaaaaa
    17521 agcacttgag caaaacctta tcccgatcgt tgtggtgaac aagattgaca aaccttcagc
    17581 tcgtccagca gaagttgtag atgaagtgct tgaattattc atcgaacttg gtgccgatga
    17641 tgagcaattg gaattcccag ttgtttacgc atcagctatt aatggaacat catcattatc
    17701 agatgaccct gctgaccaag agcatactat ggcaccgatc tttgatacga ttattgatca
    17761 tattccagcg ccagttgata attcagatga gcctttgcaa ttccaagtgt cacttttgga
    17821 ctacaacgat ttcgtaggtc gtatcggtat cggtcgtgtt ttccgtggta ctgttaaagt
    17881 gggtgaccaa gtgactcttt caaaacttga tggtaccact aaaaacttcc gtgttacaaa
    17941 actgtttggt ttcttcggtt tggaacgtcg tgaaattcaa gaagctaaag caggtgactt
    18001 gattgctgtt tcaggtatgg aagatatctt tgttggagaa accattacac caactgactg
    18061 tgtggaagct ctgccaattc ttcgtattga tgagccaacg cttcagatga ctttcttggt
    18121 caataactct ccttttgcag gtcgtgaagg taaatggatc acgtcacgta aggttgaaga
    18181 acgtctttta gcagaattgc aaacagacgt gtcacttcgt gttgacccaa cagattcgcc
    18241 agataaatgg acggtttcag ggcgtggaga attgcatttg tcaattctta tcgaaaccat
    18301 gcgtcgtgaa ggctatgaac ttcaagtatc acgtccagaa gttatcatca aagaaattga
    18361 tggtgtcaaa tgtgaaccgt ttgagcgtgt tcaaattgat acaccagaag aatatcaggg
    18421 tgcaatcatc cagtctcttt cagaacgtaa aggggatatg cttgatatgc agatggttgg
    18481 taatggtcaa acgcgtttga ttttcttgat tcctgcacgt ggtttgattg gttattcaac
    18541 agagtttctt tcaatgacac gtggatatgg tatcatgaat catacttttg atcagtatct
    18601 accggttgtt caaggagaaa ttggtggccg tcatcgtggt gccttggttt ctattgaaaa
    18661 tgggaaagca acgacatatt caatcatgcg tattgaagaa cgtggaacta tctttgtcaa
    18721 cccgggtaca gaagtttacg aagggatgat tgttggtgaa aactcacgtg ataacgacct
    18781 tggtgttaac attacaacag ctaagcaaat gacaaatgtc cgttcagcga ctaaagatca
    18841 aactgcagtt atcaagacgc ctcgtatcct aacgcttgaa gaatcattag agttcttgaa
    18901 tgacgacgaa tatatggaag taacgccaga atctatccgc ttgcgtaaac aaatcttgaa
    18961 taaagctgcg cgtgataaag ctaataaaaa gaaaaaatca gccgaatagc ttttgaagtt
    19021 aaactaacta aataaaatag caaaaagata tttaagaaaa gtcatttttt aaaggtcttt
    19081 ttgtgtttac aagttcctat ggaatataaa ccaaaataaa ggaggacgga atgttttacc
    19141 ttattatggc tgtcttgatt atatcttatt atctatacat ggctcctaaa agtgtccgta
    19201 acacccttgg gatgattggt ttagttggct tggttgctct gcttattgtt ttagcgggct
    19261 tgagttttat caaaatcatg caaatccctc ctgaattttt tattgggatg ggaatggtgg
    19321 cattgggcta ttttgcttta aaagatgtgc gtaaaatgac caaaaaaccg agagttaaat
    19381 aaaaatactt aaagattgat gattttggtt tttatacgat aaaaggactg atttgtttta
    19441 gacaatatca gtcctctttt aatagttcat tttttctgaa agaaagcctt tagccgattt
    19501 tcattgagtg tgcaactaaa aagagatgct tggtagtgac agttaagacc tctgactagt
    19561 attttatctt attttactta aaaaaacgta ccaataatct cacaaatatg ggcatgatgt
    19621 ttgtatattg gaaacggatt ctaattctaa actcaagaac ggtattttta aaatgttgaa
    19681 aaagcatcta aataaaggta aaatagaaat caaatagaat ggatagtgtg agcttagatg
    19741 aaagtgataa gtaattttca aaacaaaaaa atattaatat tggggttagc caaatcgggc
    19801 gaagcagcag caaaattatt gactaaactt ggtgctttag tgactgttaa tgatagtaaa
    19861 ccatttgacc aaaatccagc ggcacaagcc ttgttggaag aggggattaa ggtcatttgt
    19921 ggtagccatc cagtagaatt attagatgag aactttgagt acatggttaa aaaccctggg
    19981 attccttatg ataatcctat ggttaaacgc gcccttgcaa aggaaattcc catcttgact
    20041 gaagtagaat tggcttattt cgtatctgaa gcgcctatta tcgggattac aggatcaaac
    20101 gggaagacaa ccacaacgac aatgattgcc gatgttttga atgctggcgg gcaatctgca
    20161 ctcttatctg gaaacattgg ttatcctgct tcaaaagttg ttcaaaaagc aattgctggt
    20221 gatactttgg tgatggaatt gtcctctttt caattagtgg gagtgaatgc ttttcgccct
    20281 catattgctg tcatcactaa tttaatgccg actcacctgg actatcatgg cagttttgag
    20341 gattatgttg ctgctaaatg gatgattcaa gctcagatga cagaatcaga ctaccttatt
    20401 ttaaatgcta atcaagagat ttcagcaact ctagctaaga ccaccaaagc aacagtgatt
    20461 cctttttcaa ctcaaaaagt ggttgatgga gcttatctga atgatggaat actctatttt
    20521 aaagaacagg cgattatagc tgcaactgac ttaggtgtcc caggtagcca caacattgaa
    20581 aatgccctag caactattgc agttgccaag ttatctggta ttgctgatga tattattgcc
    20641 cagtgccttt cacattttgg aggcgttaaa catcgtttgc aacgggttgg tcaaatcaaa
    20701 gatattacct tctacaatga cagtaagtca accaatattt tagccactaa aaaggcttta
    20761 tcaggttttg ataacagtcg cttgattttg attgctggcg gtctagatcg tggcaatgaa
    20821 tttgacgatt tggtgccaga ccttttagga cttaagcaga tgattatttt gggagaatcc
    20881 gcagagcgta tgaagcgagc tgctaacaaa gcagaggtct cttatcttga agctagaaat
    20941 gtggcagaag caacagagct tgcttttaag ctggcccaaa caggcgatac tatcttgctt
    21001 agcccagcca atgctagctg ggatatgtat cctaattttg aggttcgtgg ggatgaattt
    21061 ttggcaacct ttgattgttt aagaggagat gcctaatgcc taagaagatt ttatttacag
    21121 gtggtggaac tgtaggtcat gtcaccttga acctcattct cataccaaaa tttatcaagg
    21181 acggttggga agtacattat attggtgata aaaatggcat tgaacataca gaaattgaaa
    21241 agtcaggcct tgacgtgacc tttcatgcta tcgcgacagg caagcttaga cgctattttt
    21301 catggcaaaa tctagctgat gtttttaagg ttgcacttgg cctcctacag tctctcttta
    21361 ttattgccaa gcttcgccct caagcccttt tttccaaagg tggttttgtc tcagtaccgc
    21421 cagttgtggc tgctaaattg cttggtaaac cagtctttat tcatgaatca gatcggtcaa
    21481 tgggactagc aaacaagatt gcctacaaat ttgcaactac catgtatacc acttttgagc
    21541 aggaagacca gttgtctaaa gttaaacacc ttggagcggt gacaaaggtt ttcaaagatg
    21601 ccaaccaaat gcctgaatca actcagttag aggcggtgaa agagtatttt agtagagacc
    21661 taaaaaccct cttgtttatt ggtggttcgg caggggcgca tgtgtttaat cagtttatta
    21721 gtgatcatcc agaattgaag caacgttata atatcatcaa tattacagga gaccctcacc
    21781 ttaatgaatt gagttctcat ctgtatcgag tagattatgt taccgatctc taccaacctt
    21841 tgatggcgat ggctgacctt gtagtgacaa gagggggctc taatacactt tttgagctac
    21901 tggcaatggc taagctacac ctcatcgttc ctcttggtaa agaagctagc cgtggcgatc
    21961 agttagaaaa tgccacttat tttgagaaga ggggctacgc taaacaatta caggaacctg
    22021 atttaacttt gcataatttt gatcaggcaa tggctgattt gtttgaacat caggctgatt
    22081 atgaggctac tatgttggca actaaggaga ttcagtcacc ggacttcttt tatgaccttt
    22141 tgagagctga tattagctcc gcgattaagg agaagtaaat ggcaaaagat aaagagaaac
    22201 aaagtgatga caagctcgtt ttgacagagt ggcaaaagcg taacattgaa tttttaaaga
    22261 aaaagaagca gcaagctgag gaagaaaaaa aactcaaaga gaaattattg agtgataaaa
    22321 aagcgcagca gcaagctcaa aatgcttctg aagcagttga gcttaaaact gatgagaaaa
    22381 ctgatagtca ggaaattgag tcagaaacga cgtcaaaacc taaaaaaccc aaaaaagtta
    22441 gacaacccaa ggaaaaaagc gcgacacaaa tcgcttttca aaaatccttg cctgttcttt
    22501 tgggggcgct cttacttatg gcggtgtcta tttttatgat cactccttat agcaaaaaga
    22561 aagagttttc tgtaagagga aaccatcaaa cgaaccttga cgaattaatc aaagctagca
    22621 aagtcaaagc atctgactat tggttaacgt tgttaacttc gcctggtcag tatgaacgac
    22681 cgattcttcg tactattcca tgggtgaaat ctgtacatct ctcttaccaa tttcctaatc
    22741 actttctatt taacgttatt gaatttgaaa tcatcgctta tgcacaagtc gaaaacggtt
    22801 ttcagcctat tttggagaat ggaaaacgtg tggacaaggt cagggcatca gaactaccga
    22861 aatctttctt gattcttaat ttaaaagatg agaaagcgat ccaacagtta gttaagcaat
    22921 taacgacatt acctaaaaaa ttagtcaaga atatcaagtc agtgtctctt gcaaattcca
    22981 aaacgacagc ggatttacta cttattgaaa tgtatgacgg taatgtagtt agagtaccgc
    23041 agtcacaact cacattgaaa cttccctatt atcaaaaatt gaaaaaaaac cttgaaaatg
    23101 atagtatagt ggatatggaa gttggaattt atactacaac acaggagatt gaaaatcaac
    23161 ctgaagttcc tcttacgcca gaacaaaacg cagctgataa agaaggagat aagcctggtg
    23221 aacatcagga acagacagac aatgattcag aaacgccagc aaatcagagt agtcctcagc
    23281 aagcaccacc atccccagaa acggtcctcg aacaggccca tggctagcta atatctaagt
    23341 tgaaaaagca atgaaaacgt tagaaattca acgattctaa cccataatga attgcctaaa
    23401 aaaaattaag tttatataac aaaaaacgta aaatgataac attttacgtt tttttatggt
    23461 ataatatttt ctgaatgatt ctgtttttta gcagttttta gaatagcaaa agtttggaaa
    23521 gtaagtgagg tcaagtgaat ggctagaaat ggctttttta ctggtttgga cattggaaca
    23581 agctcgataa aagttttagt agcagaattt atttctggtg agatgaacgt cattggtgtt
    23641 agtaatgttc caagtaccgg cgtaaaagat ggcataataa tcgatataga ggcagctgcg
    23701 actgccatca aaactgcggt agaacaagca gaagaaaaag cagggatgac aattgaaaag
    23761 gttaatgttg ggctaccggc aaaccttctt caaattgaac caacacaagg aatgattcct
    23821 gtcccaagtg agtctaaaga gataaaagat gaggatgttg atagcgttgt taaatcggct
    23881 ttaacaaaaa gtatcacacc agaacgagag gttatctctt tagttccaga agagttcatt
    23941 gtggatggct ttcagggcat tcgagatcca cgtggtatga tggggattag attagagatg
    24001 cgcgggctta tttatactgg accaagcacc attttacata atctgcgtaa aactgtagaa
    24061 agagcaggca ttaaagttga aaacatcatt atttctccgt tagctatggc taaaaccatt
    24121 ttaaacgaag gtgagcgcga gtttggagct actgtaattg atatgggagg tggacagaca
    24181 actgtcgctt ctatgcgagc acaagaattg cagtatacca atatatatgc tgaaggcggc
    24241 gaatacatta ctaaagatat atcaaaagta ttaaaaacgt ctttggctat tgcagaagca
    24301 cttaagttta attttggtca agcggagata tcagaagcta gtataactga aacagtaaaa
    24361 gttgatgtgg taggtagtga agagcctgtt gaggtaactg aacgttattt atctgaaatt
    24421 atttcagcgc gtattcgtca tattttagat cgtgtgaagc aagatttgga aagaggtcgt
    24481 ttactagact taccaggagg cattgttttg attggtggcg gtgcaatcat gcctggagtg
    24541 gtagaaattg cacaagaaat ctttggagta actgtaaagc tccatgttcc aaatcaagtc
    24601 ggtattagaa atccaatgtt ttcaaacgtt atcagtttgg tagaatatgt tggtatgatg
    24661 tctgaagtag acgttttagc acaaactgca gtttcaggag aagaactttt gcgacgcaag
    24721 cctattgatt tcagtggtca agaatcttat ttaccagatt atgatgattc aagaagacca
    24781 gaatcgacca ttggctatga acaacaagcg tcacaaacag catatgattc acaagttccg
    24841 agtgatccta aacaaaaaat ttcagaacgt gttcgtggca tatttgggag tatgtttgat
    24901 taaaagtaat aaagtgagga aataaaatgg cattttcatt tgatactgca tcaattcaag
    24961 gtgcaattat aaaagtaatt ggagtcggcg gaggtggcgg aaatgccatt aatcgtatga
    25021 ttgatgaagg tgttgctggt gttgagttca tcgcagcaaa tacagacatt caggcattaa
    25081 gctcatcaaa agctgaaacg gttattcaac taggccctaa attaactcgt ggacttggtg
    25141 ctggaggaca acctgaagta ggacgtaaag ctgctgaaga aagcgaagaa attttaacag
    25201 aagctcttac aggagcggac atggtattta ttactgccgg tatgggtggt ggctctggga
    25261 caggggctgc accggttatt gctcgtatcg ctaaaagttt gggagccttg acagtagctg
    25321 ttgttactcg cccgtttggt tttgaaggta acaaacgtgg taattttgct attgaaggta
    25381 tcgaagaact acgtgaacaa gttgatactt tgttaattat ttcaaataat aaccttcttg
    25441 agattgttga taaaaagacg cctttattag aagcacttag tgaagctgat aatgttttac
    25501 gtcagggagt tcaagggata accgacttaa ttactagtcc tggccttatc aatctcgatt
    25561 ttgccgacgt gaaaacagtt atggcaaata aagggaatgc cttgatgggg attgggattg
    25621 gttctggaga agagcgcatt gttgaggcgg cgcgtaaggc aatctattca cccctattag
    25681 aaacgactat tgatggtgca caagacgtta ttgtaaacgt tacaggaggt ctcgacatga
    25741 cacttacaga agctgaagaa gcctctgaaa ttgttgggca agctgctggt caaggcgtta
    25801 acatttggtt aggaacatct attgatgata ctatgaaaga tgacatccgt gtgactgttg
    25861 tagcaactgg agtgcgccaa gaaaaagccg agcaagtttc aggttttcgt cagcctagga
    25921 cttttaccca aaccaacgcg cagcaagtag cgggtgcaca atatgcatca gatcaagcaa
    25981 aacagtcggt tcaaccaggg tttgatcgtc gctcaaattt tgattttgac atgggggagt
    26041 ctcgcgagat accaagtgca caaaaggtaa tttctaatca taatcaaaat caaggttcag
    26101 cttttggaaa ttgggatttg agacgtgata atatttctcg tccaacagaa ggtgaattgg
    26161 ataaccatct taatatgtca acgttctcag ctaacgatga cagtgatgat gaattagaaa
    26221 cgcctccatt ctttaaaaac cgttaataat ggatttactg acaaataaaa agaagatttt
    26281 tgagactatc cgcttatcta cagaggcagc aaataggact aatgatagtg tttcagttat
    26341 tgctgtaaca aaatatgtgg atagtacaat tgcaggtcag cttatcgaag caggaattga
    26401 gcacattgcc gaaaaccgtg ttgataaatt tcttgaaaag tatgatgcgt taaagtgtat
    26461 gccagtaaag tggcatttga tcggtacctt acaacgtcgt aaggttaagg aagttatcaa
    26521 ttatgttgat tattttcacg ctctagattc tgtgagatta gctttggaaa tcaacaagag
    26581 agctgaccat cctgtgaagt gttttctaca agttaatatt tctaaagaag agagtaaaca
    26641 tggttttaac atttctgaga ttgatgaagc gattgaagaa ataggtaaga tggagaagat
    26701 acagttagtt ggtttaatga ctatggcacc agcaaatgcc agtaaagaaa gtattataac
    26761 tatttttcga caagcaaatc aattaagaaa aaacttgcag ttaaaaaaaa gaaagaatat
    26821 gccttttaca gaattgagca tgggcatgag taacgattat ccaattgcta ttcaagaagg
    26881 ctcaactttt attcggattg gtagagcttt ctttcactaa tggagaataa gatggctttt
    26941 aaagatacat ttaacaagat gatttcttat tttgacacgg atgaggttaa cgaagttgaa
    27001 gaagatgttg cagcatcaac tgataacgtt attccaagat cacaacaatc agtcagagca
    27061 agtagtcatc caaaacaaga acctagaaac aatcacgtac aacaagatca tcaagcgaga
    27121 tcccaagaac agacaaggtc acaaatgcat ccaaaacatg gtacttctga acgctattat
    27181 cagcagtctc agccaaaaga aggccatgaa atggttgaca gaagaaaacg gatgagcact
    27241 tctggtattg caaatcgccg tgagcagtat caacaatcaa cttgttcaga tcaaacaact
    27301 attgccttaa agtatcctcg taaatatgag gatgctcaag aaattgtgga tcttttaata
    27361 gttaatgaat gcgttttgat tgattttcag tttatgctag atgctcaggc tagacggtgt
    27421 ttagatttta ttgatggtgc tagtaaagtg ctctatggta gcttacaaaa ggtaggctct
    27481 tcaatgtact tactggctcc gtcaaatgta tccgtcaata tagaagaaat gactatccca
    27541 catactacac aagatattgg ctttgatttt gatatgaaaa ggcggtaaat aaatgatatt
    27601 aatactatct attcttcttc gtctgatcaa agtttacact tatttattga ttgcttacgc
    27661 actaatgtca tggtttcctg gggcatatga ttcaaaaatt gggcgtttga ttagtggtat
    27721 cgttgaacca attttaaaac cttttagagc atttaattta caatttgccg gtcttgactt
    27781 cactattttt gtcgtcatta ttagtttgaa ttttttagct caagttttgg tccgtgtgtt
    27841 tatttaatgg ttagtcatag taagatttat cagcattttc accaagaaga atatcctttt
    27901 attgatagaa tgtctgatat gattaataga gttgaagatt actatctttt agaagttact
    27961 gagtttttaa atcctagaga agtgatgatt ttaaaaagtt tgattgcttt aacagatcta
    28021 aaaatgttcg tatcaacaga ttactatcca agcgaatatg gtcgtgttat tattgcacct
    28081 ggttactatg acttagaaca aagtgatttt caaatagctt tagtagagat aagttatcag
    28141 gcaaagttta atcagttgac acatagtcaa attttaggaa ctttaattaa tgaattagga
    28201 gtaaagcgaa atttatttgg agatgttttt gttgaaatgg gatatgccca gctcatgatt
    28261 aagcgggagt tattggatta ttttttagga acaattacta aaatagctaa aactagtgtg
    28321 aaattaagag aagttaactt tgatcagtta attaggtcta ttgataacag ccagaccctg
    28381 gatattctag tttctagttt tcgattagat ggtgtagttg ctactatctt aaaaaaatct
    28441 cgaacgcaag ttatagcatt aattgaagca aataagatta aggtaaacta tcgactcgct
    28501 aataaagctt cagataatct agtcataggg gatatggtga gtatcagagg tcacgggcgt
    28561 tttactcttt tagcagataa tggagtgacc aaacatggca aacaaaaaat aacactaagt
    28621 aaaatgatac ataaataagg agatacaatg gcacttacaa cgctagaaat taaagataaa
    28681 acttttaaga caaaattccg aggatactgt gaagaagaag tcaatgaatt ccttgatatt
    28741 gttgttgatg attacgaagc tcttgtacgt aaaaatcgtg ataacgaagc aagaattaaa
    28801 gatcttgaag aaaaattatc ttactttgat gaaatgaaag agtcacttag ccagtctgta
    28861 atcttagctc aagaaacagc tgaaaaagtg aaagcaacgg ctaatgcgga agcaacaaac
    28921 ttggttagta aagcgactta tgatgctcag catttattag atgaatctaa agctaaagcc
    28981 aatcaaatgt tgcgtgatgc aactgatgag gctaagcgag ttgcgattga aacagaagaa
    29041 ttaaagcgtc aaacacgtgt gttccaccaa cgtttaatct catctattga gtcacaatta
    29101 agcttatcaa attcacctga atgggatgaa ctgttacaac cgactgcaat ctatcttcaa
    29161 aattctgacg atgcttttaa ggaagtcgtg aagaccgttc ttaatgaaga cattcctgaa
    29221 tctgatgata gtgcctcttt tgatgcaaca cgtcagttta ctccagaaga gttagaagaa
    29281 ttgcaacgtc gtgttgatga aagcaataag gagttagagg cttaccaact tgactctcaa
    29341 tctgattcta cgactgagcc agaggtaaat ctcagtgaaa cacaaacgtt taaattaaat
    29401 atctaaataa agaaaaacaa gatgatttgc ttataatcac agcaagcggg agatgatgag
    29461 agtctcgtat tccctaagta ttatcattat cattttctaa aattttcgct gaactacagt
    29521 aagcgaggag gtttgttcac cttacgagca aagagggagc ggtaagtctt atgatatatt
    29581 atcgctctga actaaggtgg taccacgagc tttcgtcctt tttgagacga ggcttttttc
    29641 gttatagtcg caagtttgtt taaaaggagt ttgaaataat gaaattaaaa gagacactta
    29701 atctaggaaa aacagccttt ccaatgcgcg caggtcttcc taataaagag ccacaatggc
    29761 aagcagcttg ggaacaagca gagctttaca aaaaacgtca agaattgaat gcaggtaagc
    29821 ctgcctttca tcttcatgat ggacctccat acgctaacgg caatattcac gttgggcatg
    29881 cccttaataa aatttcaaaa gatatcattg tgcgttctaa gtccatgtct ggctttcaag
    29941 cgccttatgt acctggttgg gacacacacg gccttccaat cgaacaagtc ttggctaagc
    30001 aaggaatcaa acgtaaggag atggatttag cagaatacct tgagatgtgt cgtcaatacg
    30061 ctttaagtca ggttgataaa cagcgagatg atttcaaacg gttaggtgtt tcggcagact
    30121 gggaaaatcc ttatgtcact ttggacccac agtttgaagc tgatcaaatt cgtgtttttg
    30181 gagcgatggc tgaaaaaggt tacatctatc gtggtgctaa acctgtttat tggtcatggt
    30241 cttcagaatc ggccttggca gaagctgaaa ttgaatacca tgacattgat tcaacctctt
    30301 tatactacgc caataaagtt aaagatggca aaggcatcct tgatactaat acgtatatcg
    30361 ttgtttggac aacaacacca tttacggtta cagcatcacg tggtttgact gttgggcctg
    30421 atatggatta tcttgtggtg aaacctgctg gttcagaccg tcaatatgtt gtggcagaag
    30481 gtcttttgga tagccttgct ggaaaatttg gctgggaatc ttttgaaact ttagccagcc
    30541 ataaaggagc tgatttagag tacattgtga cagaacaccc atgggatact gacgtagaag
    30601 aattggttat ccttggtgac catgttaccc ttgagtcagg gacagggatt gtccacacag
    30661 caccaggttt tggtgaggat gactacaatg ttgggacaaa atacaaactg gaagttgctg
    30721 tgactgttga tgaacgtggc ctaatgatgg aaaatgcagg tccagatttc cacggacaat
    30781 tttataacaa ggtaacgccg attgtcattg ataaacttgg tgatctttta ttggcacaag
    30841 aagtgatcaa tcactcttac ccatttgact ggcggacgaa aaaaccaatc atctggcgtg
    30901 ctgtaccgca atggtttgct tctgtttctg atttccgtca agatattttg gatgaaattg
    30961 aaaaaacaac cttccatcca tcatggggtg aaacacgcct ttacaatatg attcgtgatc
    31021 gtggcgactg ggttatttct cgtcaacgtg cttggggagt tccgcttcct atcttctatg
    31081 ccgaagatgg cacagctatt atgactaaag aagtgacaga tcatgtggct gatttgttcc
    31141 aagaaaatgg atcaatcatt tggtggcaaa aagaagctaa agacctatta ccagagggct
    31201 tcactcatcc agggtcacca aatggcgagt ttacaaaaga aactgatatc atggacgttt
    31261 ggtttgactc tggttcttct tggaatggag tcatgaatgc tagagaaaat ctttcctatc
    31321 cagcggatct ctacttagaa ggctctgacc agtatcgtgg ttggttcaat tcatccttaa
    31381 ttacatctgt ggctgttaat gggcatgctc catataaggc tattttatcg caaggctttg
    31441 ttcttgatgg taagggcgaa aaaatgtcta aatcaaaagg caatatcatt tctccaaatg
    31501 atgtggctaa acaatatggt gcagatattc tccgtctctg ggtagcttct gtggataccg
    31561 ataatgatat tcgtgtttct atggagatcc ttggtcaagt ctctgaaact taccgtaaaa
    31621 tccgtaacac ccttcgcttc ttgattgcta atacatctga tttcaaccct gctacggata
    31681 cagtagctta tgcagactta ggagctgttg ataagtacat gacaattgtc tttaatcagt
    31741 tggtagcaac gattactgac gcttatgagc gttatgactt tatggctatt tacaaagcag
    31801 tggtgaactt tgttacggtt gatttatcag ccttctatct tgattttgct aaagacgttg
    31861 tctatattga agctgccaat agcttagaac gtcgtcgcat gcagacagtc ttctatgata
    31921 tcttggttaa gattaccaag ttattgacgc caatcttgcc tcacaccact gaagaaatct
    31981 ggtcttatct agaatatgag tcagaagcat ttgttcaatt ggcagaaatg cctgtggcag
    32041 aaactttctc agctcaagag gatattttag aagcttggtc tgctttcatg actttgcgta
    32101 cccaagctca aaaagctttg gaagaagcgc gaaatgctaa gattatcggt aaatcattgg
    32161 aagcccattt gaccatctac gctagtgaag aagtgaaaac cttattgact gctttagaca
    32221 gcgatattgc tttgcttttg atcgtgtctc aattaaccat tgctgacttg gcagatgcgc
    32281 ctgcggatgc agtggcattt gaaggtgttg cctttatggt agaacatgcc ataggtgaag
    32341 tttgtgagcg ttcacgccgc atcgacccaa ctactcgcat gcgttcttac aatgcatttg
    32401 tctgtgatca cagcgctaaa atcattgaag aaaatttccc agaagctgtg gctgaagggt
    32461 ttgaagagag tggcaaataa gtcttatcaa gacttgaagt gacactgagt aagaagacaa
    32521 ccatcgggtc ttaggacctg atggtttttt agtagagctg attacgtcca ttagccagct
    32581 ttaacgagag acaaagtagg agaaatttag actacttttt ttatttcttt taagaaaatt
    32641 gctggcttca tataaggact attaagatag agttcacctt tcgctaataa aaaacttagc
    32701 tcttttagaa gggctaagtt ttgtctgaat agctggctgc tgtttataca gaagtttgaa
    32761 atgaaatttc aataaggtta ttcgtgtgaa gtgtttttga ttgtccagga ataacaattt
    32821 taggatcaat tttacgtttt aaaaaaaatt cgcgaatgat atcaagttca tcctccttga
    32881 tggcacggta tttatttgaa ataagtagtt cgtaatgttt tggtgcttgg gtaaaaatga
    32941 catctgtatt tcctgctgag tatacttcga ctaaataggc atcggtgttt tttagctgat
    33001 ttctgatgag ttctgggtga ctgctagtgg tattgatgag tctcataaat gtcctcctta
    33061 gtattcctgt acctattata gcactaattt aaagccttag ttagtagaat gatgcttttt
    33121 ttgccattcc ttgactcccc attgatggct ctcccgtttt aatttagcaa ttgcctctat
    33181 aggagaaaac caacctaagt tattaaaatc ttctaagggc ttagaaacag cttgaaatgc
    33241 agttacttca taaagatagg caggatggta gaagtgggta tcacgatggc gagagtagaa
    33301 atattcatca gcctgaccgt aatatgaacc aatagtagca gaaaaaccaa gttcttcaat
    33361 cagttcacgc tctagggcct gaagctgacc ttcacctgct tcaatttcac caccaggtaa
    33421 aaaccaggaa ccattaggag cttgaacaag aataattttt gtttgttcgt gattgggaat
    33481 gatagcatag acaccgtaac gtgtgacata gtctttatgt gcatttttat gtccaaatgt
    33541 tggaatcatc attttattta cctttccttt ttcttcatta tactaaattt ttgctaatta
    33601 ttcttatcaa tttgtttagt ttaataaaaa attgatagaa aaagagagag tctccttata
    33661 gaaactctct tagctaacgt taatctagag actcttcgtt aagaaaagaa tctccctctt
    33721 taacagcttt aatcgtgatg ttttcactgt ttgtcatcag tgcacgtaac tgtttttcag
    33781 ttgggtgttc taaataatag tctgtaatgg catcttcgat ataatcttgg atagtacgac
    33841 ggagcggccg agcgcccatt ttaggatcat agcctaaatc tactaatttt tctttgacct
    33901 tttgtgtcac atcaagatga atcccattat agcctaagcg ctcattaaca tcttccagca
    33961 ttaaatcaac gatatgcagc aaatgttctt tagataaagc tttgaattca ataataccat
    34021 cgaaacgatt cataaactct gggctgaaga agttgcttaa ttcaccaaga acagaacttg
    34081 tccgtccttc tctagcagca ccaaatccga cagaagcttc gcttttacct gttccggcat
    34141 tagaggtcat gataataatg gtgtccttga agctgactgt tcgcccttga ccgtctgtta
    34201 agcgaccgtc atcaagaact tgtaagaaca tgtgcatgac gtcaggatgg gctttttcca
    34261 cctcatctaa gagaataagg gaataaggat tgcgacgaac ttgttcggtt agctgtccag
    34321 cttcttcata gccgacataa cctggaggag ccccgactaa tttagcaacg gcatgttttt
    34381 ccatgtattc ggacatatca aagcgaatca tattgtcagc agagccaaag agttcaatag
    34441 ctagttgctt agacaactct gttttaccaa cacctgttgg cccaacgaat aggaaagaac
    34501 caataggacg atttggagtt cccaatccca cacggttccg gcgaatggcc ttggcaattt
    34561 tgtcaacggc atcatcttgg ccaatcacgt gagctttcaa atcattagcc aaattaacga
    34621 gttgagattg ttctttttct ttcaagtcgc caactggaat attggttttt tgttcaatga
    34681 tggcttcaat ggttttttca gtaatgatag gaatatcttg ctcatcgact ttttgagcct
    34741 gcatttcctt gtatttcgca atttgatcgc ggaaataagc tgcgcgttca tagtcttcgt
    34801 ctcgagtagc ttgcgccttg agattctcag cttcaataag acgtttgtca atttctttag
    34861 gatcaacaaa gttgagggtt agattcattt tggaaccagc ttcgtccaga aggtcaatag
    34921 ccttatcagg aagaaaacgg tcttgaatat agcggttaga taaatgagcg gcagcttcaa
    34981 tagcggctgg gctatatttg acatgatgat agtcttcgta tttcggttgg atacctttta
    35041 aaatcgtgat ggtttcctca acagaaggtt catcaacttt aacgggttgc atacgtcgct
    35101 ccaaggcagc atctttttca ataatacggt attcatttaa ggtagtagca ccaacgagtt
    35161 gcaactcacc acgggccaag gctggtttta aaatattacc agcatccata ttgccgtctc
    35221 ctgcagaacc agcaccgaca atctcatgaa tttcatcaat aaagagaatc acatccttgc
    35281 gattgcgaat ttcttccatt aatttttgca tgcgctcttc aaactgacca cggatacctg
    35341 ttccctgaac gaggctaacc acatcaagac gaatcacttg cttgccttgg agtttttgag
    35401 gaacagtacc atcaataatt ttttgagcca aaccttctac aacagcagtt ttaccaactc
    35461 caggctcacc aattagcaca ggattatttt tagtacggcg gttgaggatc tcgataacgc
    35521 gtgtaatctc ttcgtcacga ccaataacag gatcaatatt accatttctt gcaatatctg
    35581 tgacattaat cccaaactct tctaacaagc cctttgcttg ctgatttggt gtttgtggct
    35641 gagcaggtcg ttgtccgttg tagttaccac catagcgtcc tccgccattt ccattttgcc
    35701 ctgcctgagt aggtggggta tttggaagat taccaaaagc tctaaaatta ttcaaatcac
    35761 caaaaaagtc atcaaagaaa ggacttgtgg atctatcttg tgctctatat cctggggtta
    35821 ggccatttaa aatagaattg gcaggatcgg atttcatgat ttgataacaa ttttgacaga
    35881 gatcaacctg tctttgtttt ccattaacac ttgtataaag atgaatagta gattcgttta
    35941 aattacaatt ttgacacagc ataatatacc tccgagggtt tatctaaaag tcaaagtcat
    36001 ttagtcaaaa atggtcaaac tttatattca tattatagca ccacggcttg ggaaatcaag
    36061 taaaaagttt atcgctttag cgttagcttt tagagagtaa catgacagag taactttatt
    36121 aggtttccaa tgagcaaatg tttaaaatca ttgaaaagag taaaaaaagt aaaatatcaa
    36181 taaatatatt actttccttg ttagtttccc ttgttttgtg ttaaaataag agggataaaa
    36241 aacgaatagg agaaacgaaa tggaatcaca tttggtgaga atcatcaatc gtttggagtt
    36301 gatggtgaca gatggcggta atctcaagcg aaattttgaa cgcgatggtg ttgttgttgc
    36361 agaagttgcc ttttcaaatg atcctgaaaa tggacctgtt ttcacattgc gtgatgttga
    36421 ggcgcgtgag tcatattcct ttgatagcat tgacttgatt gcaatggaaa tctacgattt
    36481 attatattaa gcttgacata gtaaagagac ttgcctgagg caggtttttt tattgtcaaa
    36541 aagtattgta ttaatattca tcagatggta taattatctt tattgtaatg aacctaggac
    36601 ttatgaggag atataaatgg atttgtcatt tttgcccaaa tactgggcct actttaacta
    36661 cggtgtactt gtcaccatta tgatttcagt cagcgttgtc ttttttggaa cccttattgg
    36721 tgtcttggta accctgatta agcgtagtca tgtgaagccg ttgacctggg tcgttaatct
    36781 ttacgtttgg atctttcggg gaacacctat ggtggttcaa atcatgattg cctttgcttg
    36841 gatgcatttt aacaatatgc ctactattgg ttttggggtt ttagacttgg acttttcaag
    36901 attacttcct ggaattatta tcatttcatt gaatagcggt gcttatattt cagaaattgt
    36961 tagagcaggt attgaggctg taccaaaagg gcaattagaa gcagcttatt cactaggtat
    37021 tcgtcctcaa aatgccatgc gttatgtgat tttgcctcag gcctttaaaa atattttgcc
    37081 agccttagga aatgaattta ttaccattat taaggatagt gctcttttac aaaccattgg
    37141 agtgatggaa ctttggaatg gtgcccaatc ggtggtaacg gctacttatt ctccaatttc
    37201 ccctttactg gtggctgctt tttactactt aatggtcaca acagtgatgg cacagttatt
    37261 ggcagtctta gaacgtcaca tggcgcaagg aggtaatcat tgatgactga ggctattatt
    37321 tcaattaaag acttgcacaa gtattatggg cacaatgaag tgcttaaggg aattgattta
    37381 gacattatgc caggagaggt tgtggtgatt attggccctt ctggttctgg aaaatcgacc
    37441 ttacttcgaa ctatgaatct tttggaagta ccaactaagg gacagattag gtttgagggg
    37501 attgatatta ccgataagaa gaatgatatt ttcagcatgc gtgaaaaaat gggaatggtt
    37561 ttccagcagt ttaacctctt tcccaatatg actattttag aaaatatcac tttatcgcca
    37621 atcaaaacta agggaatggc taaagcagag gctgacaaaa cagccttgag cttgttggac
    37681 aaagttggat tatcagaaaa agccaaggct tatcctgcta gtctttctgg tgggcaacag
    37741 cagcggattg cgattgcgcg tggactggct atggatccag atgttttact ctttgatgaa
    37801 ccaacttcag ctctagaccc agaaatggtg ggtgaggtct tggctgtcat gcaagatttg
    37861 gctaaatctg ggatgactat ggttattgtg actcatgaaa tgggctttgc taaagaagtc
    37921 gcagaccgtg tgatttttat ggatggcggc gttattgtgg aagaaggaag tcctaaccaa
    37981 ctttttgatc tgactaaaga agaacgtaca aaagattttt tgagtagagt attataaatc
    38041 tgttctatga attataaaag gaagtcctaa atgatttagg acttcctttt ataatttgct
    38101 aaggcatttt ataggagatt acttttcttt ttgttaaaat aaaaagaaat agttgcatta
    38161 tttgcataga agaaagggat ctgttatggt ttcagtgatg tggtttcgta gagatttaag
    38221 gattgatgat caaaaagctt tagctcatgc tattgctagt caatcaccaa ccttatgtgt
    38281 gtttcaattt aacaaggagc aattaaatct gctacatagt cggaatcaat ctagttttat
    38341 tactagcgtt ttggctttta gaaagttatt aaaatgtgaa ggtattgact tatatctaat
    38401 gtatggagac ttaatgacct gttttgagca attattgaca caattaaaag actggacaga
    38461 tgtgtatttt aactatgatg aatctggata tggtcgttta cgtgatcaaa aagcagcaca
    38521 atttttcaaa aaaaatggga ttgccgttca tacctaccaa gatcactatt tacatggtag
    38581 tcaagagatc attaaccaat caggtcagcc ttataaagtt tttacgccct actataggat
    38641 ttggcaaaat tatccgaaag agactcctat taaagtagaa ctatcgcaag gcaggtggtt
    38701 aaatcttgag acaccagatg atgttttaag gactgttgag agctttaaag atgaaaaata
    38761 tcaggatgtt gcaacttttg atgaagcgtc aaaacaacta aatcgattta ttcaagacca
    38821 attagcagct tatcacgcta atcgagattt tccagcgcag ctagggacga gtcggttgtc
    38881 tcctttttta agaataggag ctattggcat tcgcacggta tatcacgctg tgcgtcaagc
    38941 accaaatagt ctagggcaag ctactttttt aaaagaatta gcttggagag acttttacaa
    39001 tatggtctat gtggcttatc ctgatcagaa aactcagcct atccaaaaag ccttttctca
    39061 aatcgaatgg gttaataatc ctgactggtt tcagttgtgg aaagaaggta aaacgggtta
    39121 ccctattgtc gatgctgcaa tgcttcagtt acaaaagaca ggatggatgc ataatcgttt
    39181 acgaatgata gtagcttctt ttttaactaa agatctttta tgtgattggc gtttaggaga
    39241 gcagcatttt caacaacaac taattgatta tgatgctgcc agtaatatcg gaggatggca
    39301 gtgggctgct tccactggaa cagatgctgt tccttatttt agagtattta atcctgttac
    39361 tcaaagtaag cgctttgatc ccaaaggcga gtttattaaa gcgtatttgc ctcaactaga
    39421 acacgttcca gagaagtatc tccacgaacc gtggaagatg ccaaaatacc ttcaagagag
    39481 tgtgtcctgt attattggca cagactatcc tcaaccaatt gtcgatcatg ctaaacaacg
    39541 tgaacaagct attgcaaagt atgagtgggc aaaagaaaaa gcaaaaatag aatgactgtt
    39601 ctcattataa gagtttaaac aaaaagttat catgaattaa gattatttaa tttaaaaaac
    39661 aagttgactt tatttagatt atttgatact attcagttgt ctcccttaga gaaagcgatt
    39721 tcttgttttg gagctgattt ttaccatttg gtcttagaat aggatgagta atatgactta
    39781 caacgaggta tatcaagaat ggttgcacaa taatgatctt agtgatgata ttaaagcaga
    39841 tttagcagcc ataaaagaca atgaggctga gattcaagat cgtttttaca aaacacttga
    39901 atttggaaca gcagggctaa gaggaaaact tggagcaggc accaatcgca tgaataccta
    39961 tatggttggt aaggcagcac aagctttagc taatactatt attgatcatg gacctgaagc
    40021 agttaaaaag ggcattgcgg ttagttatga tgttcgctat caatctagaa catttgcaga
    40081 attaacatgc tctattatgg cagctaacgg tattaaggcg tacctttata aagggattcg
    40141 gccaacacca atgtgttcgt acgctattcg tgctttggga tgtatctctg gtgttatgat
    40201 tacggctagt cacaatcccc aagcttacaa tggttataaa gcttattggc aagaaggttc
    40261 tcaaattttg gatgacattg cggatcaaat tgcacaacac atggctgcac taactcagta
    40321 tcaggaaatc aaacaaatgc cttttgaaaa ggctctggac agtggacttg ttacttatat
    40381 tgatgagagt attgaagaag catataaaaa agaagtgctt ggtttaacga ttaatgatac
    40441 tgacattgat aagtcagtcc gagtagtcta tactccttta aatggtgttg ggaatctacc
    40501 agtgcgtgag gttttaagac gccgcggttt tgaaaatgtt tatgtggtac ctgagcagga
    40561 aatgcccgat cctgatttta caacagttgg ctatcctaat cccgaagtcc ctaaaacttt
    40621 tgcatattca gaaaaattag gtaaggcagt tgatgctgat attttgattg caactgatcc
    40681 agattgtgac cgtgtggctt tagaagttaa aaacgcagtc ggtgactatg tttttctcaa
    40741 tggcaataaa atcggagcgc ttttatctta ctatatcttt tcacaacgat ttgacttagg
    40801 caatttaccg gctaatcctg ttttagtgaa atccattgta acaggagact tgtcacgggc
    40861 tattgctagc cattatggta ttgaaaccgt tgaaacattg actggtttta agaatatttg
    40921 tggcaaggca aacgaatatg atgtgacgaa acagaaaaac tatctgtttg gttatgaaga
    40981 aagtattggt ttttgctatg gcacttttgt tcgtgacaaa gacgctgtta gcgcctctat
    41041 gatgattgta gaaatggcag cttactacaa gaaaaagggg cagaatttac tagatgtttt
    41101 gcagactatt tatgcgacat ttgggtatta taatgaacgt caaattgcct tggagcttga
    41161 ggggatagaa ggacaaaaac gcattgcgag gataatggaa gatttcagac agacaccaat
    41221 agctagtgtt gcagagatgg cgttagacaa gacaattgat tttattgatg gctatcaaga
    41281 ttttccaaag caaaactgtc tcaaatttta tttggatgat ggttcttggt atgcacttag
    41341 accatcagga acagagccta aaataaaatt ttatttatat actattggcc aaacacaaga
    41401 aaatagtgca acaaaattag atgctattga agcagcgtgt cgcacaaaaa taaatcaggt
    41461 taattaaaca tcaaaaatgg ataacacttt tacactattt ttccatataa acaactacaa
    41521 aaggaggtga gacttttagg caccttcttt ttcttgtagg tgtcactaat caatttaatc
    41581 gactcaaacc aatactaaaa aataagtaag agtataaaga acaggcatct atttttttgt
    41641 tataatagaa aaatgaaatt aaagggagat gtcaatgaca gaactaattg atggtaaagc
    41701 cttagctcaa aagatgcaac aagagttagc agctaaagtc aacaatctaa aacaaaaaaa
    41761 aggaattgta ccaggcttag ccgttattct tgtaggtgat gatcctgcta gtcaggtgta
    41821 tgtccgtaat aaagagcgtg cagctcttac tgtaggtttt aaaagtgaga ccgttagatt
    41881 atcagaattc atttgtcaag aagagcttat tgcagtaatc gaacgttaca atgcagataa
    41941 cactattcat ggtattttag tgcagctacc cctgccaaat catattaatg ataaaaaaat
    42001 tattctcgcc attgatccca aaaaagatgt ggatggtttt cacccgatga atacaggtca
    42061 cctttggtca ggacgtccct tgatggttcc ttgtactcca tcagggatta tggaattgct
    42121 tcgagaatat aatgttaacc ttgaaggtaa acatgccgtc attattggca gatcgaatat
    42181 cgttggaaaa ccaatggcac agctcttact ggacaaaaat gcaacagtca cgttgacaca
    42241 ttcaagaaca cgtcaattag aagaagtatg tcgctgtgca gatgtgttga ttgtggcaat
    42301 cggacaaggt catttcataa caaaacaata tataaaagag ggtgcgatag tgattgatgt
    42361 gggaatgaac cgtgatgata atggcaagct aattggagat gtggcctttg atgaggtggc
    42421 agaagttgca gcgaaaatca cccctgtacc aggaggtgtc ggtcctatga cgattgctat
    42481 gttgctagag caaacttatc aatctgctct ccgtagtact cataaatagt tatcattaga
    42541 caattcaaca taagagaata taagaagtca gcaatggctt ccttttttgt ttttctgctc
    42601 actatttatc cagaaaaatg gtataattgc attagtatgc aaagggacta tgtcaggtcc
    42661 aactaagtga aaggtggctt gatggcagat tatttaaccg tcactcattt gacgaaatat
    42721 ttaaaattaa aatttgaccg tgacccttat ttggaacggg tttatctgac tggtcaagtg
    42781 tccaattttc gaaaacgacc aactcatcaa tatttttcct taaaggatga aagtgctgtg
    42841 attcaagcca ccatgtgggc aggagtctat aaaaaactag gattcgacct agaagaaggc
    42901 atgaaaatta atgtaattgg gcgagttcaa ctttatgaac ctagtggctc ttactctatc
    42961 gtgattgaaa aggcagagcc agatggtata ggtgccttgg ctttgcagtt tgaacaatta
    43021 aagaaaaaat taacggcaga aggttatttt gagcaaaaac acaagcagcc tttgccacag
    43081 tttgtatcaa aaattggggt cattacaagt cctagtggtg ctgtgattcg agatattatc
    43141 acaaccgttt cacggcgttt tccaggggtt gagattttat tattcccaac taaagtacaa
    43201 ggtgatggag ccgcccaaga agtagtggct aatattcgaa gagccaatca aagagaggat
    43261 ttggacttgc tcattgtcgg ccgtggaggt ggctcgatag aagacctttg ggccttcaac
    43321 gaggaaatag tggttcaggc tatctttgaa tcgcaacttc cagtgatttc gagtgtgggt
    43381 catgaaacag acacgacttt agcggatttt gtggcagatc gcagagcagc cacaccaaca
    43441 gcagcagcag agttagcaac gcctattaca aaaacagatc ttatgtcttg gatagtagag
    43501 cggcaaaatc gttcctatca ggcctgcttg aggcggatta agcaacggca agagtgggtg
    43561 gataaactct cgcaatctgt tatttttagg caaccagaac gattatatga tgcctacctg
    43621 caaaaaattg atcgccttag catgaccctg atgaatacga tgaaagatcg tctgagttcc
    43681 gccaaagaaa acaaggttca gttggaccat gccttggcca atagtcaatt acagacaaaa
    43741 atcgagcgat accaagaccg tgttgctacc gctaagcgtt tactgatggc taatatggct
    43801 agtcaatatg atagtcagct ggctcgcttt gaaaaagcac aggatgcttt gctatcactg
    43861 gataccagcc gaattatcgc ccgtggttat gcaatgattg aaaaaaatca agcactggta
    43921 gtctctgtaa gtcagataac aaagggtgat cagttaacca ttaagatgcg tgatggacaa
    43981 ttagatgtag aggtaaaaga tgtcaaaaac gaaaacattt gaagaaaatt tacaagattt
    44041 agagactatt gtgaacaaac ttgaaaatgg ggatgtccct ttggaagaag ctatttcaga
    44101 atttcaaaaa ggaatgcttc tctcaaaaga acttcaaaag accttacaag cggcagaaaa
    44161 aacactcgtc aaagtgatgc aagctgatgg cacagaagta gatatggatg attaatggac
    44221 aaattagcta gaattgacga agccattcgt cgctactata agacgactag taacggtgta
    44281 tctgaagagt tgattgatgc tatcttgtat tctgttgaca gtggtggcaa gcgcattcga
    44341 ccacttatct tattagagat gattgaggga tttggggtat cgttacagaa tgctcatttt
    44401 gatttggctg cagcccttga aatgattcat acaggaagtc tgattcacga tgatttgcca
    44461 gccatggata atgatgatta ccgacgtggt agactgacca atcacaaaca atttggagaa
    44521 gccacggcca ttttggcggg agatagctta tttttagacc cctttgggtt aatagctcaa
    44581 gctgaattaa atagcgaggt taaagtagcc ttaatccaag aactttccct agcttcagga
    44641 acttttggca tggttggtgg tcagatgcta gatatgaaag gggaaaatca agctttgagt
    44701 ctccctcagt tgtcactgat tcacctcaat aaaaccggaa aattattaac ttttcctttt
    44761 aaagcagcag ctcttgtaac agaacaggct attactgttc gtcaacaact agagcaggcg
    44821 ggaatgctca tcgggcacgc ctttcagatt agggatgata ttttagacgt gacagctagc
    44881 tttgaagatc ttggaaaaac gcctaaaaag gatttatttg cagaaaaagc tacttaccca
    44941 agtttattgg ggttagaagc ctcctgccaa ctgttgacag agagtttaga tcaggctttg
    45001 acgatttttc agacactcga aagcgatgta ggctttaagc ctcaaataat tacaaaactg
    45061 atagaagggt tacgacttaa tgcctaaaga aagagtagat gtgttagcct ataagcaagg
    45121 attatttgag accagagagc aagctaagcg tggtgttatg gctggcttag tggtctctgt
    45181 gattaatggc caacgctatg acaagccagg tgacaaaatt gacgatggga ctgagttaaa
    45241 acttaaaggt gaaaaactca aatacgtcag tcgtggtgga ttaaaactgg aaaaggggct
    45301 gcacgttttt ggtgtatcag ttgctaatca aattgggatt gatattggcg cttcaacagg
    45361 tggctttacc gatgttatgt tacaagatgg ggccaaacta gtctatgccg tcgacgttgg
    45421 gactaaccag ttggtatgga aactcagaca agatccacga gtaagaagta tggaacagta
    45481 taactttcgt tatgctcagc cagaggactt taatgaggga cagcctgtat ttgccagtat
    45541 tgatgtgtcc tttatttctc tcagcttgat tttgccagcc ctgcataacg ttttgtcaga
    45601 tcagggacag gttattgctc tcattaaacc gcaatttgaa gctgggcgcg agcagattgg
    45661 taaaaaaggc atcgtcaaag acaaacagat tcatgaaaaa gtgattcaaa aggtcatgga
    45721 ttttgcctca ggttacggat ttacagttaa ggaacttgat ttttccccta ttcagggtgg
    45781 tcatggaaat atcgaatttt tagctcatct tgttaagtca cagacacctg aaacgttagc
    45841 cccacatctg attcaggaag ttgttgccaa ggcacataag gagtttgaga aacatgaaaa
    45901 aaagtgaacg acttgaatta atcaaaaaaa tggttttgac gcatccaatt gagacgcaac
    45961 atgacctctt aagattattg gcagagcatg ggttagaatt aacccaggct actatttcaa
    46021 gagacatgaa tgaaattggt atagtaaaga ttccttctgg cagtggacgt tacatctatg
    46081 gtctttctca agatagtgga aaaaagatcg ttcaaggacc tagatcgata aagagcacta
    46141 ttttagctgt atctgacaaa acaaaaggtt tagaacaaca cctctattta aaagtcgtac
    46201 ctggcaatag taaattaatc aaacgttatt tattagcaga tttttcaaaa gctattttta
    46261 gtcttattgc tgatgatgat agtttattgt taattgcaaa gtctccttca gaagcagata
    46321 tgatacgcca agaaattttg ctttggatgc agggtataac ctagaaaaaa taggggagaa
    46381 agtgatgtta ttagaaattt ccattaaaaa ttttgctatt attgatgaaa tttccctaaa
    46441 ttttgaaaat ggtatgacgg ttttgacggg tgaaactgga gcaggaaagt ctattatcat
    46501 tgatgctatg aatatgatgc ttggtgcgcg tgctagtaca gaagtgattc gtcgtggagc
    46561 taataaagca gaaattgagg ggttcttttc ggtagatgct accccagaac tggttgcgtg
    46621 tctggaatca tcaggtattg ctatggaaga agaactgatt attcgccggg atatctttgc
    46681 caatggcaga agcgtgagcc gcattaatgg tcagatggtt aacctagcca ccttaaaaca
    46741 ggtcggacag tttttggtag atattcatgg gcaacatgac caagaagaat taatgcgacc
    46801 acagctccac cagcaaattt tggacgcttt tggtgataag gcttttgagc aattgaaaga
    46861 gaactatcag cttatttttg atcgttataa aagcttgcgt cgccaggtta ttgacaaaca
    46921 aaaaaatgaa aaagaacata aagatcgtat cgacatgttg gcttttcaga tagcggagat
    46981 tgaagctgct gccttgagtc gaggtgagga cgaccggtta aatcaagaac gtgatcgctt
    47041 aatgaaccac aaacaaattg ctgataccct gaccaatgcc tacgtcatgc tagataatga
    47101 cgatttttca agtctatcca atattcgctc tagcatgaat gacttactat caattgagca
    47161 gtttgattca gagtacaaag ggatgtcgac ttcgatttct gaagcctatt atattcttga
    47221 agaagtgagc aagcaattat cagataccat tgaccaactg gattttgatg gtgggcgatt
    47281 acaggaaatt gaatttcgct tggatacact caatagctta acccgtaaat acggtggtaa
    47341 tgtgaatgat gtgcttgact actacgataa tattatcaaa gaataccagc tattaacagg
    47401 agatgacttg tcttcagggg atctagaggc agaattgaaa agcttggaaa aacaattggt
    47461 tgctgctgct tcggagttaa gtgtctctcg ccatcaattg gcggagcaat tagaagctga
    47521 gattaaagct gaacttaagg aactttatat ggaaaaagca gatttcaaag ttcactttac
    47581 cacatcaaaa ttcaatcgtg atggtaatga aagcttggaa ttttacatct ctactaatcc
    47641 aggcgaagga ttcaagcccc ttgtcaaggt ggcttctgga ggagagttat cacgccttat
    47701 gttagccatc aaggctgcta tttctagaaa agaagataag acgagtattg tttttgatga
    47761 agtagataca ggtgtttctg gtcgcgttgc gcaagctatt gcccaaaaaa tttataaaat
    47821 tgggcgacat gggcaagttc tcgctatttc acacttacct caagttattg ctattgctga
    47881 ttatcagtat tttatttcta aagaaagtaa agaagagtcc accgtttcta aggtaaggtt
    47941 attaactcct gaagaacgtg tggaagaaat tgctagcatg attgcaggaa cagatatgac
    48001 acaagctgct cttacgcagg ctcgtgaact acttgccaaa cattaagatt atgtttggta
    48061 tttgatattt tcataattag atgacattga ttttttaggt gctaaaataa tcattgtgtc
    48121 agaccatttt tcctctgtta atctggcttg gtttgccttg acaaacctct gagaaatagt
    48181 agaatagata agagaatttt aagctgaggg gatagaaatg actagtaaaa aagcgtgttt
    48241 atcaagcatc attgtgttag caagtttaac gtgtggaaat gatactgtta gtgccaatca
    48301 tctctcagca actggagata agtttgatga ttgctcaaca cttgttgaaa aagatgtggc
    48361 ccctaaagat gaacttgaga tgttagcatg gtcctcgtct caaacaactg atgatgctga
    48421 cagagactat gaagattttc tcgatgatga ttcttttaat gatgattctt ttatttctca
    48481 aaatgaaact gataagatgt ttgagaattt aactgatgat aggttattag atgaattaga
    48541 tgaagaagat acaattgagc cagagcaaaa tgtaataatg cctagtgacg atgagctatt
    48601 tgatttaact gatgctgttg agacacgcct tactgtttct agtgctcccc atttagaggc
    48661 tgaattgccg aaaccacatt tgaggagcct atcagataca gcactgcggt ctggtgaaat
    48721 tagaggacat ttagataaca aactggacgc tttgtctgta acagctacaa agttagcatt
    48781 aacgatggct caaaaatttg atttgacaac gcatgtctat tctataggtg aaagctttag
    48841 tgaagtatta gctgctcatt atgaagacag aaaagcagaa tcagcttttt ctaagaaaaa
    48901 gagatttcac cttcctattg ctactccaga tgttgttata gaggagttaa ggcgcctggt
    48961 ctcttctatt ggaagttcaa aagaagatgt ttcagttcct tataatcgga agctaggtat
    49021 ggcagttgca aaaagaaaaa tagccctgcc acaaacggga gaggagttct cttattatcc
    49081 agctttactt ggtttaatga tattaggatt aacgccgatt atgataccaa agaagataaa
    49141 taattaggta ataagttatt gtagttgtaa aagacggggc aaatgttcaa gcgtgaaaag
    49201 gttgactttt gcctcgtttt gtatctcgtt attgctaagg cattttcttc gagcagctag
    49261 tgctcgccat acctatctac cgagtgacta ttcaatagca ctaccagttt tgctttattt
    49321 agcaaggagt gttagaataa agaggtgaga aactagttta gaatggaaaa atacatggga
    49381 actataaaaa ttgttacaga ttcatcaata actattgaac cagaattaat taaagcttta
    49441 gatattactg tagtgccttt atctgtcatg attgatagca aattatattc tgataatgat
    49501 ttaaaagaag aaggtcattt tttgagtctt atgaaggcta gcaaatccct tcccaaaacc
    49561 agtcagccac cggttggtct ctttgctgaa acttacgaaa atttggtcaa aaaaggagta
    49621 actgacattg ttgctattca cctctcacca gctttatcag gtactattga agcttcccgt
    49681 cagggggctg aaatcgctga ggcacctgtt acagttttag attcaggatt tactgatcaa
    49741 gccatgaaat ttcaggtcgt tgaggcagca aagatggcaa aagcgggtgc tagtttgaat
    49801 gagattttgg ctgctgttca agctatcaaa tcaaaaacag agctctacat tggtgtatct
    49861 accttagaaa atttagtaaa aggaggtcgt attggtcgtg tgacgggtgt tttgagctcg
    49921 ctacttaatg tcaaagttgt tatggcactt aaaaatgatg agttaaaaac gcttgtcaaa
    49981 ggtcgaggta ataaaacatt tacgaaatgg ttagatagtt acctcgctaa aaatagtcat
    50041 cgcccgattg cagagatagc tatttcttat gctggtgaag ctagcctggc attaacctta
    50101 aaagaaagaa tcgcagctta ttacaaccac tctatttcgg ttttagaaac aggctctatc
    50161 atccaaacac atacaggaga aggagctttt gcggttatgg ttcgttatga ataatcgtca
    50221 tttatttagt gggatatttt tctttgttat tagtttatgt ctggcctttt tattgctaaa
    50281 tattattatc cctaagtcaa attcacgttt gaaaaagagt gattttctga caaaagaaca
    50341 agtagctatc caatatgttg ctataggaga ttcattgaca gaaggagtag gtgatctaac
    50401 tcatcaaggt ggttttgttc ctttgttaac gaatgatctc agtgaatatt ttaaggctaa
    50461 tgttaatcat caaaattacg gcgtatctgg tgataccagt caacagattc ttgataggat
    50521 gataaaacaa aagcagatac agttatcttt aaaaaaagca gatataatga cgttaaccgt
    50581 tggtggtaat gatgttatgg cagttattcg gaagaattta gcggatttgc aagtttctag
    50641 ttttagaaag ccagctcgtc agtatcaaaa acgattaaga cagattatcg agttagccag
    50701 aaaagataat aaagatcttc ctatttttat tttaggcatc tataatccgt tttatttgaa
    50761 ttttccagaa ctaactgata tgcaaaaagt gattgatgac tggaatatca aaactaagga
    50821 ggttgttgga gaatacgatc gtgtgtactt tgtgccaata aatgacctct tgtataaagg
    50881 gataaatgga caagaaggaa ttgtccattc ttcaggagat caaactacaa ttgtcaatga
    50941 tgccttgttt actggggacc attttcaccc aaataatact ggctatcaaa tcatgtcaaa
    51001 tgcagtaatg gagaaaatta aaaagcatga aaaaaaaatc aaaccttaat tggtggaagt
    51061 ggagcttctt atgtctgctt gcttttaata ccgccttttt gatggttatt gcaagtcgtc
    51121 tcattcaagt aagagaacct gagtcagaac tgattgctaa aaaaccagtt aaaaatatca
    51181 aaattggtac atttgtcaca accagagaac agcttaacga aactgtagca agttatctta
    51241 aagattatca gactaaaaaa atgtcttata aattttatgc gacatcttct tctattttgt
    51301 ttgaaggaac ttatcaatta ttagggtatg aagtcccttt atatatctat tttcaacctc
    51361 atcgtctaga aaatggagct gtccaactac aggtgatctc tttttcagtg ggaactctgc
    51421 cactcccaga aaaagatgtt ttgcagtatt tgaaatcaag ctacaaattg ccaagctttg
    51481 ttaaagtgat gccaaatcaa tcagctatcg ttgttaattt acaagatatc caaaatgatg
    51541 ctaaggttta tttaaaggcc aagaaaattg atttatttaa tgatgaaatc agttttaaca
    51601 tctataagaa ataagctcaa ataacgttaa atacttgacc taatcgcttt ttttagatat
    51661 gataatatct gttaaagcat taagcttact aattatttgg aggatttgtt aaatggctaa
    51721 caaacaagat ttaatcgcaa aggttgcaga agcaactgaa ttgactaaaa aagattcagc
    51781 agcagcagtt gatgccgtgt tttctacaat cgaagctttc cttgctgaag gtgaaaaagt
    51841 acaattgatc ggttttggta acttcgaagt acgcgaacgt gcagctcgta aaggtcgtaa
    51901 cccacaaact ggtgcagaaa ttgaaattgc agcttcaaaa gttccagcct tcaaagctgg
    51961 taaagctctt aaagacgctg ttaaataatt cgtctagaaa aatcttgttg ctatcgatgt
    52021 ttattgatag cgacaaggtt ctttttttat atttggggca taaagttagt agttttctct
    52081 tcagttaagc taagttctaa tgcatgcact ctataatggt cagtcatcct tctattagta
    52141 tatcatttcc gcaggtaggg ggagtttttt tcatatttgc aaatagttga agttaggatt
    52201 tttgaaagca aaaggtaagt ttaaaaaaag tttatttag
//
LOCUS       DAUGHY010000007        50006 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.007, whole genome shotgun sequence.
ACCESSION   DAUGHY010000007 DAUGHY010000000
VERSION     DAUGHY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50006
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.007"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            6..1211
                     /gene="sstT"
                     /locus_tag="VH752_000385"
     CDS             6..1211
                     /gene="sstT"
                     /locus_tag="VH752_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEQ8288759.1"
                     /translation="MYDLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLFVGG
                     LKAIAPLLVFALVSQAISHQKKGKQTNMTLIIVLYLFGTFASALVAVLTAYLFPLTLV
                     LNTPVNTELSPPQGVAEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKAASQ
                     ETKHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLILVLVGTM
                     VFVALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKIGLS
                     KDTYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSACGAS
                     GVAGGSLLLIPVACSLFGISNDLAMQVVGVGFIVGVIQDSCETALNSSTDVLFTAIAE
                     NAFWKRKKA"
     gene            complement(1266..1940)
                     /locus_tag="VH752_000386"
     CDS             complement(1266..1940)
                     /locus_tag="VH752_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEQ8288760.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGIEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFVIPKSW
                     EGQSLSELDVRRKYELNVIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(1950..3341)
                     /locus_tag="VH752_000387"
     CDS             complement(1950..3341)
                     /locus_tag="VH752_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEQ8288761.1"
                     /translation="MSGGNMKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQN
                     GPNTVYLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVST
                     FALKRKMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFSIEAFAAIVIMIDFIPRFG
                     WKNGIFNSIFLAVSAFCNAGFDNLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVD
                     LGVAFKKYFFERPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIAN
                     FSLHQQLMVSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVT
                     TAAITFLLFKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPH
                     IAPIPLLFESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKE
                     KTIQYATTDILVG"
     gene            complement(3411..4124)
                     /gene="rsmG"
                     /locus_tag="VH752_000388"
     CDS             complement(3411..4124)
                     /gene="rsmG"
                     /locus_tag="VH752_000388"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEQ8288762.1"
                     /translation="MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAI
                     TEENEVYLKHFYDSIAPILQAFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            4274..4831
                     /locus_tag="VH752_000389"
     CDS             4274..4831
                     /locus_tag="VH752_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEQ8288763.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            4878..5774
                     /gene="htpX"
                     /locus_tag="VH752_000390"
     CDS             4878..5774
                     /gene="htpX"
                     /locus_tag="VH752_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEQ8288764.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMIKALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
     gene            6008..6541
                     /locus_tag="VH752_000391"
     CDS             6008..6541
                     /locus_tag="VH752_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEQ8288765.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VRYDKGLYLLDYQLSYEVILPSSRSMVPVCLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            6808..7494
                     /gene="covR"
                     /locus_tag="VH752_000392"
                     /gene_synonym="csrR"
     CDS             6808..7494
                     /gene="covR"
                     /locus_tag="VH752_000392"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HEQ8288766.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARIRAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            7500..9002
                     /gene="covS"
                     /locus_tag="VH752_000393"
                     /gene_synonym="csrS"
     CDS             7500..9002
                     /gene="covS"
                     /locus_tag="VH752_000393"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HEQ8288767.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITTRRFL
                     KPLHNLHEVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMIRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQIVDGYHLQMKVESELNEGSVFILHIPLAQSKES"
     gene            9217..9711
                     /gene="nrdR"
                     /locus_tag="VH752_000394"
     CDS             9217..9711
                     /gene="nrdR"
                     /locus_tag="VH752_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEQ8288768.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVVQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            9695..10870
                     /locus_tag="VH752_000395"
     CDS             9695..10870
                     /locus_tag="VH752_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEQ8288769.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAVSIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQGDLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSRRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            10871..11773
                     /gene="dnaI"
                     /locus_tag="VH752_000396"
     CDS             10871..11773
                     /gene="dnaI"
                     /locus_tag="VH752_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEQ8288770.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLSDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYSFADLERKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            11836..13146
                     /gene="der"
                     /locus_tag="VH752_000397"
     CDS             11836..13146
                     /gene="der"
                     /locus_tag="VH752_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEQ8288771.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTIDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            13353..16451
                     /locus_tag="VH752_000398"
     CDS             13353..16451
                     /locus_tag="VH752_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ8288772.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSPFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQDLIEFLWRLASNTDKGDNENIFPNHARHL
                     RLPSGFFEEGIHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVYRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQFDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIRRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQIRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMLEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMKDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMD
                     YQGKSGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLPGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNESRASMSIEEIKEILGLN
                     TH"
     gene            16694..17296
                     /locus_tag="VH752_000399"
     CDS             16694..17296
                     /locus_tag="VH752_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288773.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIARNEEFTAKATSRQAPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            17336..18664
                     /locus_tag="VH752_000400"
     CDS             17336..18664
                     /locus_tag="VH752_000400"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEQ8288774.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMELIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEEGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDMALVADHLKTFSGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            18710..19192
                     /locus_tag="VH752_000401"
     CDS             18710..19192
                     /locus_tag="VH752_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8288775.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEERIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            19304..20878
                     /gene="mltG"
                     /locus_tag="VH752_000402"
     CDS             19304..20878
                     /gene="mltG"
                     /locus_tag="VH752_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEQ8288776.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAAKRTAQLYAEYKRQDAFQKESIAHNNKTAKHFQAIKGAVMTSEALKPTLLSE
                     KENSSLKTTNKRVVQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAVTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQIQ"
     gene            20951..21433
                     /gene="greA"
                     /locus_tag="VH752_000403"
     CDS             20951..21433
                     /gene="greA"
                     /locus_tag="VH752_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEQ8288777.1"
                     /translation="MAEKTYPMTLTEKEQLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVQEVGTTD
                     KDTYHIVGAAGADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTN
                     "
     gene            complement(22057..22980)
                     /gene="yidC"
                     /locus_tag="VH752_000404"
     CDS             complement(22057..22980)
                     /gene="yidC"
                     /locus_tag="VH752_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ8288778.1"
                     /translation="MKLTLNRILFSGLALSILFTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMTFLKPVFEP
                     INKRIKQASSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMGIDLGSRSLVLTAIIAALYFFQSWLSMMAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITTYLLKPRLHKQIKEEYAKTPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(23062..23340)
                     /locus_tag="VH752_000405"
     CDS             complement(23062..23340)
                     /locus_tag="VH752_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEQ8288779.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGDIYGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGN"
     gene            23601..24338
                     /locus_tag="VH752_000406"
     CDS             23601..24338
                     /locus_tag="VH752_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEQ8288780.1"
                     /translation="MVITSKANQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYETPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            24377..24877
                     /locus_tag="VH752_000407"
     CDS             24377..24877
                     /locus_tag="VH752_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ8288781.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLHRKT
                     LQSHNR"
     gene            24892..25581
                     /locus_tag="VH752_000408"
     CDS             24892..25581
                     /locus_tag="VH752_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEQ8288782.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFVASGAARKNTPAALPIFLIYSALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
     gene            25759..26001
                     /locus_tag="VH752_000409"
     CDS             25759..26001
                     /locus_tag="VH752_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEQ8288783.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
     gene            26179..26973
                     /gene="racE"
                     /locus_tag="VH752_000410"
     CDS             26179..26973
                     /gene="racE"
                     /locus_tag="VH752_000410"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEQ8288784.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSIQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSLAPLVGKIDTLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLL
                     NYFDINGNYHQKAVKHRFFTTANPEIFQEIASIWLKQKINVEHVTL"
     gene            26970..27956
                     /locus_tag="VH752_000411"
     CDS             26970..27956
                     /locus_tag="VH752_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEQ8288785.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRYQSPLSLITFIIDMINQETQREIKVIPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQGFGDTILIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNAKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEAEWPGYIATQPKG
                     ENGFGYDPVFIVGETGRHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            27935..28456
                     /locus_tag="VH752_000412"
     CDS             27935..28456
                     /locus_tag="VH752_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEQ8288786.1"
                     /translation="MASKTIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVA
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYPSLSKEFKR"
     gene            28453..28914
                     /locus_tag="VH752_000413"
     CDS             28453..28914
                     /locus_tag="VH752_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ8288787.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDFPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            28911..29657
                     /gene="xerD"
                     /locus_tag="VH752_000414"
     CDS             28911..29657
                     /gene="xerD"
                     /locus_tag="VH752_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEQ8288788.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLVGERVNQDKLLL
                     YQNSIANLSLSAKKRKLSTANQFLYYLYQIKYLNSYFRLTDTMKVMRTEKQQAAIINT
                     DIFYQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLS
                     QILIPFLEQQLVGKEVYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFIL
                     KEKLAGKSIIELSDILGLKSPVTLEKYYKS"
     gene            29657..30361
                     /locus_tag="VH752_000415"
     CDS             29657..30361
                     /locus_tag="VH752_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEQ8288789.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVS
                     QALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKTNHTV
                     IERDDYKIEDMMASIKQRLEKENVISLSAIFEECQTLNEVISIFLASLELIKLHVVFV
                     EQLSNFGAIILRKEKK"
     gene            30358..30909
                     /locus_tag="VH752_000416"
     CDS             30358..30909
                     /locus_tag="VH752_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEQ8288790.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAGLLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            31031..31753
                     /locus_tag="VH752_000417"
     CDS             31031..31753
                     /locus_tag="VH752_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ8288791.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            31750..32013
                     /gene="yidD"
                     /locus_tag="VH752_000418"
     CDS             31750..32013
                     /gene="yidD"
                     /locus_tag="VH752_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEQ8288792.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            32191..32739
                     /locus_tag="VH752_000419"
     CDS             32191..32739
                     /locus_tag="VH752_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEQ8288793.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
                     ALRQQGFQGLELKHTYEHDKLK"
     regulatory      32848..32972
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            33051..33614
                     /locus_tag="VH752_000420"
     CDS             33051..33614
                     /locus_tag="VH752_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ8288794.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVTKYMMTMVIPFN
                     LVEGLIFAITFYFVYIASKPILERYLH"
     gene            33616..34269
                     /locus_tag="VH752_000421"
     CDS             33616..34269
                     /locus_tag="VH752_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ8288795.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQVLLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILVIGLSRIYLGVHYPSDILAGFVLGFGILQFIYPFYKQKRFEWRFLLKQD"
     gene            34562..35482
                     /locus_tag="VH752_000422"
     CDS             34562..35482
                     /locus_tag="VH752_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEQ8288796.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKETVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            35521..36075
                     /locus_tag="VH752_000423"
     CDS             35521..36075
                     /locus_tag="VH752_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ8288797.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLLNAELILAGHEAVDQYVTEPVRAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADV"
     gene            36208..37542
                     /locus_tag="VH752_000424"
     CDS             36208..37542
                     /locus_tag="VH752_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEQ8288798.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQEQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            37620..38411
                     /gene="pflA"
                     /locus_tag="VH752_000425"
     CDS             37620..38411
                     /gene="pflA"
                     /locus_tag="VH752_000425"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEQ8288799.1"
                     /translation="MTEKDYEQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCH
                     NPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
                     ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGE
                     FKWRELGIPYQLEGVKPPTKERVQNAKNLMQTESYTEYMNRIHQS"
     gene            38542..39477
                     /locus_tag="VH752_000426"
     CDS             38542..39477
                     /locus_tag="VH752_000426"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEQ8288800.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDIAEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            39553..40206
                     /locus_tag="VH752_000427"
     CDS             39553..40206
                     /locus_tag="VH752_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEQ8288801.1"
                     /translation="MITIFHSDKLTRQPFFQDLINYLDQHDHVILREIKKAFPNVTGI
                     DKAIESYVQAGYIRRENKRYGINLPLVSSDQQLALDTMLFVDTCSAMYENILAVVFET
                     QLTNQTNHVMIKEKTNITRSQLTLANYFYRLKRGEKPSAEQMDLYDLLGDVNQEYALK
                     YMTTFLLKFTRKDLVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(40251..41288)
                     /locus_tag="VH752_000428"
     CDS             complement(40251..41288)
                     /locus_tag="VH752_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ8288802.1"
                     /translation="MLFMVNKKRRPLLKSHRHLLRLYTSLILLLVSLMGLALSLGESH
                     LSFLDLIHVFLGKSSHAISFIVINIRLPRILAACLGGGSLALSGLLLQRLTRNPLADS
                     GVLGITIGAGISLAIVVSFSFFEQAHISHYLPLFAMLGAIVTTFSVYWLSLTKQGQID
                     PTRLILTGVAVTTILSSLMVALVGHINRYKVDLVINWLSGQLIGDDWPTLSVIAPLLL
                     CFWLLTYSQAHFLNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFI
                     GLIAGHFSTYLVGSNHKITIPISILIGMILLLVADTVGRVYLVGSNIQTGILVSLIGA
                     PYFLYLMAKTK"
     gene            complement(41249..42301)
                     /locus_tag="VH752_000429"
     CDS             complement(41249..42301)
                     /locus_tag="VH752_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ8288803.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALVLAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKGYHQLRLVLAGAMVSILLSALGQGITNYYHLTNAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYLLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            complement(42291..43223)
                     /locus_tag="VH752_000430"
     CDS             complement(42291..43223)
                     /locus_tag="VH752_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ8288804.1"
                     /translation="MKKLTLLLTLCLTTITLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVTATD
                     LEAITTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGRIFNKEGKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEVIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIGDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLESLREAILSSEN"
     gene            complement(43249..44031)
                     /locus_tag="VH752_000431"
     CDS             complement(43249..44031)
                     /locus_tag="VH752_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8288805.1"
                     /translation="MTTISAEDLTIAYEQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGVVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAMQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFQIKPVLVDDPIHNCPIVLTYQLQ"
     gene            complement(44277..45722)
                     /locus_tag="VH752_000432"
     CDS             complement(44277..45722)
                     /locus_tag="VH752_000432"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEQ8288806.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGLSFERLSYDSRQ
                     VDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMA
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
                     TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVVINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIAKHYL"
     gene            45810..47444
                     /locus_tag="VH752_000433"
     CDS             45810..47444
                     /locus_tag="VH752_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEQ8288807.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGLIFSVIMYLGSPLFASLSGGDDTLVPIMHSLSLAVFIFPVMSVIRGIFQGHNN
                     IKPYAISQIAEQLIRVIWMLLTTFLIMKLGSGDYASAVTQSTFAAFIGMVASMGVLGY
                     YLWKQGLLAAIFSKPDHTVSIDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWTFVNT
                     MTLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEDRAIHLFVAVLFQTLLMALYT
                     LFSPMLQALFENRKAIYYFAYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLYQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYAFKPTGRLTSLLYLLII
                     GGVGMTVYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            47612..48241
                     /gene="upp"
                     /locus_tag="VH752_000434"
     CDS             47612..48241
                     /gene="upp"
                     /locus_tag="VH752_000434"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEQ8288808.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEAHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            48465..49055
                     /locus_tag="VH752_000435"
     CDS             48465..49055
                     /locus_tag="VH752_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEQ8288809.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLAYGFIDEIMENNELK"
     gene            49550..49843
                     /locus_tag="VH752_000436"
     CDS             49550..49843
                     /locus_tag="VH752_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEQ8288810.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIIYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKGYQPPSY"
BASE COUNT        15948 a         8718 c        10172 g        15168 t
ORIGIN      
        1 aaaaaatata tgatttatgg gttagggtta gtctaatcaa aaaaataggt atcggtgttg
       61 taataggagt catgttgggc attttggctc ctgatcttac aggttttagt attttaggaa
      121 agttatttgt tggtggttta aaggctatcg cacctttgct tgtatttgct ttggtttccc
      181 aagccatctc ccatcagaaa aaagggaaac agactaatat gacattaatc attgttctgt
      241 atttatttgg tacctttgcg tcagctttag tagcagtact gacagcttat ctattccctt
      301 tgacgttagt gttaaataca cctgtaaata cagaattatc accacctcaa ggtgttgctg
      361 aagtttttca gtcacttcta ttaaaattag tggataatcc gatcaatgct ttagctactg
      421 ccaattatat tggtgtgcta tcatgggcaa ttatttttgg actagcatta aaggctgcaa
      481 gtcaagaaac aaagcatctt ataaaaacag cggcagaagt tacttcacaa atagttgttt
      541 ggatcatcaa tctggcacct attgggatta tgagtctggt gttcactacg atttctgaaa
      601 acggtgttgg aatcttatct gattatgctt ttttgatact agttttagta ggaacaatgg
      661 tttttgtcgc cttagtggtt aatccactta ttgcggtttt gattacacga caaaatcctt
      721 atccccttgt cttgagatgt ttacgtgagt caggtcttac agcatttttc acacgaagtt
      781 ctgcagccaa tattccagtc aatatgcaat tatgccaaaa aattggattg agtaaagata
      841 cttattcagt gtcaattcca cttggagcaa ctattaatat gggtggggca gcaattacca
      901 tcaatgtcct aacgcttgct gctgtacaca cttttggtat tcctattgat tttctaacag
      961 ccctcttact tagcgttgtt gctgctgtct ctgcttgtgg tgcttctggt gttgctgggg
     1021 gatcgttgtt acttattcct gtagcatgta gtttgtttgg catttcaaat gatcttgcga
     1081 tgcaggtggt tggtgttggt tttattgttg gtgttatcca ggattcttgt gagacagcct
     1141 taaattcgtc aaccgatgtt ctttttacag ctattgctga aaatgctttt tggaaacgta
     1201 aaaaagccta gctaggttta acatagtaaa aaacatcctt ggaaaacgcc aaggatgttt
     1261 ttttattatt taagatagcc aaggtaatca aatttttcaa aggtgtggtc attagcgatg
     1321 gctacaatga tagtattcgg ttctaaaggt tcaaaaggtt tgacattggt atctaaggtt
     1381 ttaacttctt tttgacgcat gccaatcaca tttaactcat atttacgacg aacatctagt
     1441 tcggaaagag attgtccttc ccagctttta ggaatgacaa attcaatcat ggaaatgcca
     1501 tgttcgaggt agatgatgct ttcgatatga cgtcttaaca gatttgaagc tacacgtttt
     1561 ccagaatcac gttcaggggt gatgacttta gttgccccga tgccatatag aacttcttca
     1621 aaaattttat ttttagcctt ggcaataatg gttggaacac cgagtttttt acagtgcatg
     1681 actgcaagaa cagaagattc caagttattc cctgaagcga taacgacagt atcacaatgt
     1741 tcaatcccta cagctaatag aaattctttg tcagtaatat ctccaacagc ggctttagtg
     1801 accaagtcgg ccacctcttt aacatgactt tctctgatat caatagcaat aacgtcttga
     1861 tcaaaattac ttaattctct ggcaacagta cggccaaaaa taccaaggcc aagaacgccg
     1921 acagttttac gttttaacat gttttctcct taacccacta aaatatcagt agtggcatat
     1981 tgaatggttt tttcttttcg ttgaatcaga ctgatgagga cagtaattgg gccaacgcgg
     2041 ccgacgaaca tcaagacaat gacaataaga cgtccagcag tagacaattg tggtgtcaaa
     2101 tccatagata ccccaactgt cgcgatggca gaaatagatt caaataagag tggtatagga
     2161 gcgatatgag gctccacgct aagtaataaa ataaaaccta tcataagaac tgcaaaaaag
     2221 aagataagaa ctgtcatggt ctgcttaatt gtcttatttg cgataattcg gttacgaaag
     2281 gtaacttctg attgccctga aagttccgct ttaaagagta agaaagtaat ggcagcagtc
     2341 gtcactttaa taccaccagc tgttccacca ggagcaccac caataaccat ttgaatcata
     2401 taaagaatgt tagtcggtgc tagggtatcg ttataagaga ttgttgcaaa gccagctgtt
     2461 ctcatagtga ctgtttgaaa aaatgaaacc ataagttgct gatgtaaact aaaattggca
     2521 atggttttgc tgttatcttt ttctaaaaac caggttaaga aggttccaag aaaaagaata
     2581 acagctgtgg tttgtagtac tagacgagac tgattggata atttacgaaa agttgcccca
     2641 taacaatgag gtctttcaaa aaagtatttt ttaaaagcta ctcctaaatc aacccaaacc
     2701 gcaaaaccaa gtccaccaga gataatcaag aacgtaatga taacatttaa tgtaggattt
     2761 aacataaagt cttttaaact agaagatcct aaattgtcaa aaccagcgtt acaaaatgcc
     2821 gacacagcta gaaaaatact attgaagata ccatttttcc acccgaagcg ggggataaag
     2881 tcaatcatga taactatcgc agcaaaggct tctatactaa aggttacttt ataagcaaaa
     2941 aaaaggtagt gctttaaatc tttgctatca cctcgattta acgctgattg gagcaaggtt
     3001 tgatcgctca atcgcatttt tcgcttgagg gcaaaagtac tcacagctat taacgtcacc
     3061 aagccaagac caccaatctg cattagtgcc atagcaatgg tttgtccgat accgttatat
     3121 acttcagcga cgggaacaac agaaagtcca gtcacacaaa ccatggaaac cacattaaag
     3181 aaatgatcaa gatagacggt attaggacca ttttgataat gggtaaaggg catggataac
     3241 aagagagtcc caatcagaat cacaatggca aaactaaatg ttaagcgctg tgtgactgaa
     3301 agagatttga taaaagagcg tttcatgttt cctccagaca taatatctcc tactataata
     3361 acaaaaataa gttaaaaaag ctaagtttca tcttagcctt tggtactatt ttatagaggt
     3421 tttttgttag gcaagccagc ttttcttgga tacttgttag gtgtttcctt tttcttctca
     3481 acaatggtga tgaagcgaga atctccattt ggcaattggt agctatggtt tttaatgact
     3541 ttaccaaaaa gaagacaaag agcattctta gcttcttcta actcttggtc agcagcttgt
     3601 gcctttaagg cgattaattt tcctccaatt tttaaaaagg ggatggttaa ttcagataga
     3661 acctgcatcc gagcaaccgc tctggcagta acgacatcaa attgacctcg aaaggccttg
     3721 tcctgtccga aatcttctgc tctaccatga aaaaaatgga cattttccaa gcccaattcc
     3781 tgagcaagga gtgtcagaaa agaaatccgc ttattaagag aatcaataat ggtaacctca
     3841 aggttcggga aaagaatttt catgggtagg ctaggaaaac cagctccagc tccaatgtcc
     3901 aataatttga ttggttcatt agctaaaaag gcctgtaaaa taggagcgat ggaatcataa
     3961 aaatgcttga ggtaaacctc attttcttct gtaatagctg ttaaattaat tttagtgttc
     4021 cattccacta gtaatttaaa gtaggtgtca aattgttctt tttgtttcga tgataaggaa
     4081 aaaccatctt cctctagtgt tcggtaaaag tcttggggtg tcatttgtat cctctgtttt
     4141 tgatagttgt taccatttta tcataaaata gcccataaca aaatccaata aatcatcaga
     4201 aataacacct cattacttgc tattagccta gaaattgcta taattgagac acaaagatta
     4261 aaggagaccc actatgccaa cagttttgat tatcttagtc gttttaggag tgcttgcgct
     4321 atggttaatg attagttaca atagcctggt taaatctcgg atgcacacaa aagaagcttg
     4381 gagtcagatt gatgtgcaat taaagcgtcg taatgacttg attccaaatc ttattgaaac
     4441 cgtaaaggga tatgctagct acgagcaaaa gacatttgaa aaaatcactg atttgcgtgc
     4501 gcgtgttgcg aatgcctcaa ctcctcaaga aaccatggcg gcttctaacg aattgagtaa
     4561 acaagtgacc agtttgtttg ccgttgctga aaattaccca gacttaaaag ctaacgaaaa
     4621 cttcttgaaa ttacaagaag agttgaccaa tacggaaaat aaaatctcat attctcgtca
     4681 actctataat tcaacaacgt ctaattacaa ccttcaatta gaatctttcc caagcaatat
     4741 cgctggtaaa ttatttggtt ttaaaccaag tgaattctta caaacaccag aagctgaaaa
     4801 agaagttcca aaagttgaat ttaactttta aatatgaaat tatcatctag taggcaatca
     4861 taagagaaag gagaattatg ctttatcaac agatttcaca aaataagcaa cgaacagttg
     4921 ttttattagt tgttttcttc gctctcttag cacttattgg agcgtcagca ggctatctgc
     4981 tgttagataa ttatgccatg ggcttggttc ttgctcttgt cattggggtg atttatgcta
     5041 ccagtatgat ttttcaatcc accagtcttg taatgagtat gaacaatgct agagaagtta
     5101 cagaaaaaga ggctccaggc ttcttccata ttgtcgagga tatggctatg gtggctcaga
     5161 ttccgatgcc gagagttttt attattgaag atccttcttt aaatgctttt gcgacaggat
     5221 ctagccctca aaatgctgct gttgcagcaa cgacaggttt acttgaagtt atgaatcgtg
     5281 aagaacttga aggtgttatc ggccatgaaa ttagccacat ccgaaattat gatatccgta
     5341 tttcgactat tgctgtagct ttggcgagtg ctgttaccgt tatttctagt atcggtgggc
     5401 gtatgctatg gtatggtggc ggttctcgca ggcaaagaga tgacggagat gatgatgttt
     5461 taagaatcat tacgttactt ttgtcgttat tatctttgct tttagcgccc ctagtagcaa
     5521 gtttgattca gttggctatt tctcgtcaaa gagaatatct agctgatgct agttctgttg
     5581 aactaacaag aaatccccaa ggtatgatta aggctcttga aaaattgcag ttatctcagc
     5641 caatgaagca tcctgttgat gatgctagtg cggccttgta tattaatgag cctcgcaaaa
     5701 aaaggagctt cagttcatta ttcagcaccc atcctcctat tgaggagagg attgaaaggt
     5761 taaaaaacat gtgagcgttt ggggtttttg ctcaagcctc acttttttat gtctttgggc
     5821 agataatcta aaatggcagg aattatcaaa aatatctgaa caaaaagaaa aaaatgagtg
     5881 aaacaatggt taaaaataga atgatttaaa tcttaaaatt aaaaaacgtc agaaaataaa
     5941 tgcaaaacta ttgacaaatc ttttagatag tagtaaaaat aaatgagagt tttattagga
     6001 gaagatgatg ttagctattt ccgaaatcag aaaacaccca gacggtctct cgtttgatcg
     6061 tttatgtgat gtcaaaagta tgttacttga gcgtgaccaa cagattattg acataaaggc
     6121 agttaaagct gtcggtaatg ttcgctatga taaggggctt tatctgttag attatcaatt
     6181 gagttacgaa gtgatattac cgtctagccg ttctatggtt ccggtctgtt taagtgaagt
     6241 ccaacacatt caagaattat ttatagaggc gacagattta gctgataaga aagagttggt
     6301 tgaagacaat ctggtattag ttttagacaa agacgctatt aacctcgaag aaagtattgt
     6361 ggataatatc ttacttgcta ttccgctaca ggtcttgaca gaggaagaaa agaaatcaaa
     6421 agagttgcca gcaggtcaaa attgggcagt tttgacagaa gaagactacc agtgtctaaa
     6481 agaagaaaag caaaaagaaa ataatccttt tgctagcttg caagggttgt ttgatgaata
     6541 aaactgaata ttaaagatgt ctgtaatttt tatttgaaaa aacagctatt ctttgatata
     6601 ctctttagag aatatggtta cttatctaaa gtagaaggta ttttgaccat agagggcaaa
     6661 gaaggtaatg actattagat gcttcatatt ttattcgcta gaagactatt tgaccattag
     6721 aactgaaata gtctaggata tgagatggat ttcatagttt tctttctggg agaaaaagat
     6781 agattaagag gataagggtt ggtataaatg acaaagaaga ttttaattat tgaagatgaa
     6841 aagaatctgg ctagattcgt ttctcttgag ctgcaacatg agggttatga agtcattgtt
     6901 gaggtcaatg gtcgtgaagg gttagaaact gctttggaaa aagagtttga tttaatcctg
     6961 cttgacttaa tgttaccaga gatggatggt tttgaagtga cccgtcgttt gcaaaccgaa
     7021 aaaacaacgt atatcatgat gatgactgcg cgtgattcta ttatggatgt ggttgcaggt
     7081 ttagaccgcg gtgcagacga ctatattgtt aaaccgtttg ccattgaaga actacttgcc
     7141 cgtattcgtg ctattttccg ccgtcaagat attgaatctg agaagaaagt gcctagtcaa
     7201 ggcatttatc gagatctagt tttaaatcca caaaaccgtt cagttaatcg tggcgacgat
     7261 gagatttctc tcactaaacg tgaatatgat ttgcttaata ttttgatgac taatatgaat
     7321 cgtgtcatga cacgtgaaga attattgtca aatgtttgga aatatgatga agctgttgag
     7381 actaatgttg tagatgtcta tattcgttat ctccgcggca aaattgacat tccaggcaag
     7441 gaatcttata tccaaacagt gcgtggcatg ggatacgtta ttcgtgagaa ataagtcata
     7501 tggaaaatca gaaacaaaaa cagaagaaat ataaaaactc gttaccaaaa cgactatcta
     7561 atatcttttt tgttcttttt ttctgcattt tctctgcctt tacactgatt gcctatagtt
     7621 caacaaacta tttcttattg aagaaagaaa agcagtcagt ctttcaagct gtaaatattg
     7681 ttagagttcg tctttctgag gtggactcta attttacatt agagaactta gcagaagttt
     7741 tgtacaaaaa cgataaaaca catctgagaa ttgatgacag aaagggcagt cgagtcatta
     7801 ggagtgagcg cgatatcaca aatactctag atgcgaatca agatatttat gtctataaca
     7861 ttgataaaca gatgattttt accacagata atgaagaatc atctcctggc ttgcatggtc
     7921 ctatcggtcg ggtgtatcac gaccatatcg aagatcagta tcgtgggttt tccatgacac
     7981 aaaaggtata ttctaatcgg actggaaaat ttgtgggcta tgttcaagtc tttcatgatt
     8041 taggcaatta ttatgtcatt agagcaagac tgctgttttg gctactagtt gttgagttat
     8101 ttggcacaag cttagcctat ttaatcatct taattactac gcggcgcttt ttgaagcctt
     8161 tacacaattt acatgaagta atgcgtaata tctctgaaaa tcctaataat ttaaacctgc
     8221 gctcagatat ttcgtcagga gatgaaatcg aagaattgtc tgttatcttt gacaatatgt
     8281 tagacaaatt ggagacacat actaagttgc aatcacgttt tattagtgat gtcagccatg
     8341 aattacgaac accggttgcg attattaaag ggcatattgg tctcttacaa cgttggggta
     8401 aagatgacag tgatattctt gaagaaagtt tgacagcaac agcgcatgag gctgaccgta
     8461 tggcaatcat gattaatgat atgttagaca tgattcgcgt acaaggctca tttgagggac
     8521 atcaaaatga tatgacagtt ttggaagatt ctattgaaac tgttgttggt aattttagag
     8581 ttttaagaga agattttatc tttacatggc agtcagaaaa tccaaaaacg atagcccgta
     8641 tttataaaaa tcattttgag caggctttga tgattcttat tgacaatgct gtgaagtatt
     8701 cccgtaaaga aaagaaaatc gcgattaacc tttcagtgac tggcaaacaa gaagctattg
     8761 ttagagttca agataaaggc gaaggaattt ctaaagaaga tattgaacat atctttgaac
     8821 gcttttatag aacggataaa tcacgtaatc gaacaagtac tcaggctgga ttagggattg
     8881 gcttgtctat tctcaagcaa attgtagatg ggtatcattt acagatgaag gttgaaagtg
     8941 aattaaatga aggttcagtg tttatcttac atattccttt ggcccagtct aaagagagtt
     9001 agagtagctt aaggcattgt taatgtgaca ggtatagtat gtaatcatta gataatagta
     9061 gcagtctttt gccacaactg gttagaagct gatgctattt ttttagcaat gatggatgac
     9121 atgaaagaaa tactgtcaat cgcgatgcct tttgttatcc ttgagaatat ggtaaaatag
     9181 tgtattagat taatttgata gaattggggt gtttgcgtgc gttgtccaaa atgtaattac
     9241 cataaatcga gtgttgttga cagtagacaa gctgaagacg gcaacaccat tcgtcgtcga
     9301 agagaatgtg aacagtgtca tactcgcttt actacctttg agcgtgttga agaactccct
     9361 ttattagtca tcaaaaaaga tggcacacga gagcaatttt ctagagataa aatattaaat
     9421 ggggttgtcc aaagtgcgca aaaacgacct gtctcgagca cagatattga aaatgtcatt
     9481 tctcgtattg aacaagaagt tcgaacaacc tacgaaaatg aagtgtcaag tactgctatt
     9541 ggtaatttag tgatggatga gttggccgaa cttgatgaaa tcacatatgt acgctttgct
     9601 agtgtctaca agagcttcaa ggacgttgat gaaatcgaag aacttttgca acaaattacg
     9661 aatcgcgtac gcggaaagaa aaaacgttta aataatgatg aaaccaattg atacttttac
     9721 atatctaaaa cgcaacaaag taactcttga ttcggtgact ttaatacagt tgtactttcc
     9781 aattattgga agtgacgcag tctctatcta tcaatacttt attcactttt ttgacgatgg
     9841 tttacaacgt cataaattca gcgatatttt aaatcaccta cagtttggta tgaaacgttt
     9901 tgaagatgct cttgccatat tgacagcaat ggaattagtg agtgtgtatc agctatcaga
     9961 tacatatctc attacgttgc accagccgct tagccgtgac ttgttttttc aacatccagc
    10021 ttattctcga ttattagaac aaaagattgg agaggtagct gtctctgagt tgcaggtaac
    10081 tgtcccaagt caagcaagaa atatttctaa gcgattttcg gatatttttg gggtgcaagg
    10141 agatttgacg aacgtacctc aaaagccaca aaagaatttt gacctttcaa gtttccagca
    10201 attaatggtt cgtgatgggt tacaatttga agataatcaa aaagatatca tcagccttta
    10261 cagtattgct gaacagtatg atatgacgtg gtttgatact tatcaaatag ctaaggcgac
    10321 ggctgttaat gggaaaattc gacctgaacg cttattggct aaaaaaaatc aaagcatgac
    10381 aaaaccaagc aaagaaaatt tttcacaggc tgagcaaatt attttgcgag aagcaaagca
    10441 agacagtgct ttagtctttt tggaaaaaat taaaaaggct agacgtgcaa cgattactaa
    10501 ggatgaacgc attttattgc aaacattggc caaaatgaat tttcttgatg atgttatcaa
    10561 tgtcatggtg ctttatacct ttaataagac aaaatctgct aatttacaga agagttatgt
    10621 cttaaaaatg gccaatgatt ttgcctacca aaaggtgtca acggctgaag aagctatagt
    10681 ggttttgcga gccttcacgg atcgtcaaag tcgtcggcaa tcaaaagtaa aaacaagtca
    10741 aagcaatgtg cctaagtgga gcaatcctga ttaccaagag acaacaagtc aagaagagca
    10801 ggcgaaacta gatcagttca aacaggctgc tttgaagagg ttagaaaacc taggaaaagg
    10861 gggtgactag atggaaaaga ttggagaaac catggcgaaa ttgggtcaaa acactcgtgt
    10921 taatagcgac cagttgattc aaaccatttt agcggatcct gaagtagcaa gcttcatcag
    10981 tcaacatcac ttatctcaag agcaaattaa ccttagctta tctaagttca atcaattttt
    11041 ggtggaacgc cagaagtatc aactcaaaga tccctcttat attgctaagg gctatcagcc
    11101 tattctagct atgaatgagg gctacgctga tgtgtcttat ttggaaacca aagaattggt
    11161 agaagctcaa aaacaggcag ctatctctga acggattcaa ctggttagtt tgccaaaatc
    11221 ctatcgccat attcatttat cagatattga tgttaataat gcgagccgta tggaagcctt
    11281 ctctgctatt ttagattttg tggaacaata cccatcagca gagcaaaaag gcttatattt
    11341 gtacggagat atgggaattg gcaagtctta cttgttggct gctatggccc atgaattgtc
    11401 tgagaaaaaa ggtgtgtcaa cgacccttct acattttccg agctttgcca ttgatgtaaa
    11461 aaatgccatt tcaaacggct ctgttaaaga agaaatcgat gcggtcaaaa atgtaccagt
    11521 gctcatttta gatgatattg gtgcagagca agccacttct tgggtccgtg atgaggtgtt
    11581 gcaggtgatt ttacaatacc gtatgttaga ggaattgcca accttcttta cctctaatta
    11641 cagctttgcg gatttggagc gaaaatgggc aaccatcaaa ggaagtgacg aaacttggca
    11701 ggccaaacgt gtcatggaac gcgtgcgtta tttggctaga gagtttcatt tagaaggggc
    11761 taaccgccgt taagtttgag atagcaatca agaagaatag ctacacgtat gagtgagtga
    11821 aagaaaagga gaatcatggt tttacctacc gttgccattg tgggccgtcc aaacgtgggc
    11881 aagtccacct tatttaatcg aatcgcagga gagcgcattt caattgtaga agacgttgaa
    11941 ggggttactc gtgaccgtat ttatgctact ggggagtggc ttaaccgcca attttcattg
    12001 attgatacag gtggtattga tgacgttgat gccccgttta tggagcaaat caagcaccag
    12061 gctcaaattg ccatggaaga agctgatgtt atcgtttttg ttgtttctgg caaagaaggg
    12121 gttacggatg cagatgaata tgtgtctaaa atcctatacc gaaccaatac tccagtgatt
    12181 ttagctgtca acaaagtgga taatcctgaa atgcgtaatg acatttatga tttttattca
    12241 cttggtttag gggatcccta tccagtttct tctgttcatg gtatcggaac aggggatgtc
    12301 ttggatgcga tcgttgaaaa cttgccagta gaagaggctg aagaaaatga tgatattatt
    12361 cgcttcagtt taatcggtcg tcctaatgtt gggaaatcta gcttgattaa cgctatttta
    12421 ggtgaggacc gtgttatcgc tagccctgtc gcagggacaa cacgtgacgc tattgacacg
    12481 cattttacgg atgctgacgg tcaagaattt accatgattg acacggctgg aatgcgcaaa
    12541 tctggcaaga tttacgaaaa tacagagaaa tattctgtca tgcgtgccat gcgtgccatt
    12601 gaccgctccg atgtggtctt gatggttatc aatgctgaag aaggtatccg tgaatacgac
    12661 aagcgtatcg ctggctttgc acacgaagct ggtaaaggaa tgattatcgt ggttaacaaa
    12721 tgggatacca ttgacaagga taatcatacc gttgctaagt gggaagccga tattcgtgac
    12781 caattccaat ttttgacata cgctccgatt atttttgtgt cagctttgac gaagcagcgc
    12841 ctcaacaaat tgcctgactt gattaaacgt atcagtgaga gtcagaataa acgtatccca
    12901 tctgctgtcc tcaatgatgt tatcatggat gctattgcca ttaatccaac gccaacggat
    12961 aagggtaaac gtttgaaaat tttctatgcc actcaagtgt cagtaaagcc accaacattt
    13021 gtcgtctttg ttaacgaaga agaattgatg cacttctctt atctacgctt cttggaaaac
    13081 cagattcgcg cagcctttac ctttgaaggg acaccgattc atctgattgc tcgcaaacgg
    13141 aaataagaaa tgaacatcaa ctaaaatgct gacctaagaa ataatctcat cattgataag
    13201 gactgtgttg tgtatgttac aggagacagt cctttttata atacctttaa gggtgtagac
    13261 ggaagattag tagcaaacaa agaaaaagga ttttgacaag aaatatcagt cggtttttgc
    13321 tataatgaaa ggataagcaa aaaggtggaa aaatgatggc tagattaatt ccaggacgag
    13381 taaggaatga aggtattaag ctttatgagc aaggtttagt ctcattccaa gatgataata
    13441 aaggcattct tcaaatagaa gtggagactt atcaggttca atatggagca gatgatgagg
    13501 atatcacttg tcaatgcgac acatttcata tgaaacatta ttgcaagcat attgcagcag
    13561 tcgagtattt tttaaaaaat gatcaaaaag gtaagctttt tttaaaacag ttaacaaatc
    13621 aaacaaaaat caaagaaaca acaaagaaaa tgacatcttt tggtagtctc tttttagata
    13681 gcttagccat gaatgaagat gattctgtta aatacaggct gtctgctctt ggtagtcgaa
    13741 gccctttttc gtcagattac tggtggagtc tgaaaatcaa tcgtttacca gatgatcgtt
    13801 cttatgtgat tcgagatatt aaaggctttt tgcagcttat taaaaaagaa ggcttttatc
    13861 agattggaaa aaattatttt gaacaacttt catggctgca gtttgaccca tccagtcaag
    13921 accttattga gtttttatgg cgcttagcat ctaatactga caaaggggac aacgagaaca
    13981 tttttccaaa tcatgcaagg catttgcgct tacctagtgg tttttttgaa gaaggtattc
    14041 actatttgac gtcactttat gactttactt ttgaggggcc ttctcaaact tatcatcatc
    14101 tttttgtccg ttctttggaa gcagaggcag gcctttatga gtttaaagtg gaagtgtatc
    14161 gcaaatctat tgaattgcaa attgctgaaa aaaatgtgca atacctcttt gataatgact
    14221 atcttcttta tcaagatacc ttttatcact tgacgttaaa gcaacgaaaa atggttcagg
    14281 ctattcgtag tctgcccatt gaggccgact tggctaaaca tattcatttt gatttggatg
    14341 atcatgcgaa attagctgca agcttatcag attttaaaca gatcggtcta gtagaggctc
    14401 ctaaaagctt tgctattaga gactttgagg tgacttttca atttgatttg ttaaatcgag
    14461 atgaaatcag ttgtcagctt atgtttgatt atggcaatta ccaagttagt gacaaagcaa
    14521 gtttagaagc acttcctttt gctagtcatt taaaaaaaga agaaaaaatc aatcgttctt
    14581 tacttgcttt tggcttctcg ccacaatttt actcaaaaaa gcgactaaca agtgcaaaag
    14641 agctctatac tttttttgaa gaaacagttc catgttttga gcgcttaggt aatgttgcgt
    14701 tgagtacagc tatccaagca ttacaagtga aagaaatgcc caagatcgct attagacgca
    14761 atcagggatt actagatatt tcgtttgatt tttcaaccat tattgaaaat gatattgacc
    14821 aagctgttac agctcttttt caaaataatc cttattttgt gagtcaaaca ggtcaattag
    14881 tggtttttga tgatgaaact caaaaagtta gcaaaagttt acaagaatta agagcaagac
    14941 agctaaaaaa tggtcacttg caactggatg gcattagagc tctacaagta tccaaacttt
    15001 ttgaaggcat gacatctgtt catttttcaa aggaattaga agagttagcc tatcatttgc
    15061 agcatcctga aactttttca ataaaaccgt taccagttaa ggctcaaatg cgagattatc
    15121 aacgcaacgg tgttcagtgg ttatcaatgc tcaatcatta tggctttggt ggcattttag
    15181 cagatgacat gggacttgga aaaactctcc agacacttgc ttttttagct agtcacctga
    15241 aatcagatag taaagtgctc attttatctc catctagtct tatctataat tggtttgatg
    15301 aatgtcaaaa attcacacct cagctagatg ttgtagtttc ttacggactt aaacaaatca
    15361 gagatcaaat catcgaagaa ggtcatcaaa taaccataac cagctattcc tcctttagac
    15421 aggattttga gacctatcaa gcgtttcact acgattatct tatcttagat gaagctcaag
    15481 tgataaaaaa tgcgcaaact aagatttcac actgtttacg tgcgtttaat acagccaatt
    15541 gttttgcgct ttcgggcact cctattgaaa ataaaatgct tgaaatttgg tccattttcc
    15601 aaattgtttt gccagggttg ctaccgacta aaaaagagtt tttaaaatta acagctgagc
    15661 aagtatcgcg ctatatcaaa ccctttgtga tgcgtcgtaa aaaagaagat gtcctaccag
    15721 aattacctga tttaattgaa actaattatt caaacgagat gacagatgaa caaaaagcta
    15781 tttacttagc gcagttaagg cagatgaaag atcaaattcg aaattctagt gatgtggata
    15841 ttagccgtca aaaaattgag attttatctg gtatcactcg attgaggcaa atctgtgaca
    15901 ctccaagtct ctttatggat taccaaggca aaagtggtaa gctagatagt ttgcgaatct
    15961 tattgacaca aattaaagaa aatggtcatc gggctttgat tttttctcaa tttagaggga
    16021 tgctggactt ggccaagcaa gagatgactg ctctaggatt gacctcttat caaatgacgg
    16081 gatctacccc tgccaatgaa cgtcaagaaa tgactagagc ctttaataac ggttccaagg
    16141 atgctttttt gatttccctc aaggcaggtg gagttggaat caaccttccg ggagcagaca
    16201 cagtgatttt aatcgattta tggtggaatc ctgctgttga aatgcaggca atcagtcgcg
    16261 cctaccgtat tggtcaaaaa gaaaacgttg aagtatatcg cttaatcact cgagggacga
    16321 ttgaagaaaa gattttagag cttcaagaaa gtaagcgcaa tttggtgact acagttttgg
    16381 atggcaatga aagccgagca agtatgtcca ttgaagaaat aaaagaaatt ttggggctaa
    16441 acactcatta atcttccttg attttagtga cattaaaaac cctttaaaga agatgacacc
    16501 aaaagcacct tatataacct cgttaaaacg cttctttttg aaatatcttt attgatatag
    16561 cgattgtcat ttgctagtaa tgattaaaac atttagaaaa agacaagtca tagaatcatc
    16621 aaggaatgct ttgaaaaata acagtattta agttataata gaggatgcta caagaaggga
    16681 gaatcaaaaa aggatggtaa aaaatcagtt tcctttagtt gcagatggca ttgctattag
    16741 tgatccagca aagcaaatgg ctctgtatga aaatgaagat ctcattacca acatccgtgg
    16801 ctattaccag gacaaggagt atgatgatat tgctagaaat gaggagttta ctgctaaagc
    16861 aacttctcgg caggcccctt ctagtaagcg cttttgtagc aatgatgaaa aacaccatta
    16921 tgtcaaagag gcgcgtcaaa aagcaaagca agatctcaag gaaaaaagac aggcttatct
    16981 tgccaaagaa atggcctatg ttcctaaaca ggtttctaaa aagcaacagc ctgcggattc
    17041 ttctccttct caaaagcaag caacaacaga aatgagtcgt tttacaaaaa agcttcatca
    17101 agacaactat attttagctg agttgccaaa agaatataaa gagccaaaaa atcttcctca
    17161 acaaggaact acaaaaaaaa ataattacga ttttttaaaa tccagtcaaa tttacaataa
    17221 taaagaaatg agacaacaac gagaaaaaac aattgcgcaa gagcttaatc ttagtaggtt
    17281 tgaggatctt ccttaataaa tctactcagc gtatgcagat gacaaaggag ttaagatgtc
    17341 aaaaacgtat cattttatcg gaattaaagg gtcaggaatg agtgccttgg cccttatgtt
    17401 gcatcaaatg gggcataagg tgcaaggtag tgatgttgaa aaatactatt tcactcaaag
    17461 aggattggaa caagcgggca tcactatctt accttttagc gaggacaaca ttacgccaga
    17521 tatggaactt atcgttggga atgcctttcg tgaaaacaac aaagaagtgg cctatgcttt
    17581 gagacaccag attcctttca aacgttatca tgacttttta ggtgatttta tgaaatcatt
    17641 tatcagtttt gcagtagcag gtgctcatgg taaaacttct acaacgggtt tattgtcaca
    17701 tgtcttgaaa aatatcacag acacatctta tttaattggt gatggtactg ggcgtggatc
    17761 tgctaatgca cagtattttg tttttgaatc agacgaatac gaacgccatt ttatgcctta
    17821 tcacccagaa tactctatca ttactaatat tgattttgac catcctgatt atttcacagg
    17881 aattgctgat gtgcgaaacg ctttcaacga ttatgctaag caggtcaaga aagctttgtt
    17941 tgtttatggt gaagatgacg aactaaaaaa gatcgaagca cctgcaccta tttactatta
    18001 tggttttgaa gagggcaatg attttatcgc ttacgacatt acgcgtacta cgaacggatc
    18061 ggactttaag gtaaaacacc aaggagaggt cattggtcag tttcacgtac ctgcttatgg
    18121 aaaacataac attttaaacg ctacagcagt aattgcaaac ttgtttgtag ccggcattga
    18181 tatggctcta gtggcagacc atcttaaaac tttttcaggt gtgaaacgtc gttttacaga
    18241 gaaaattatc aatgacacta ttattattga tgactttgct catcatccaa ccgaaattgt
    18301 ggcaacaatt gatgcggcta gacaaaaata cccaagtaaa gaaattgtag caatatttca
    18361 gccacatact tttactcgca ctattgcctt attagaggat tttgcttgtg ctttaaatga
    18421 agctgacagt gtttatttag cccagattta tggttctgca cgtgaagtag ataaaggtga
    18481 agttaaggtt gaagatttgg ctgctaagat tatcaaacca tcacaagtag taacggtgga
    18541 aaatgtctct cctcttcttg atcacgataa tgctgtttat gtttttatgg gagctggaga
    18601 tattcagcta tatgaacatt cttttgagga attacttgca aatttaacta aaaataacca
    18661 ataacatgtc ctgccttagg caggttttct cattctaaag gagtttgata tgttgattag
    18721 acaggttcaa ggatccgatt tggaagttat tgctaccatt gaaagtgaca atttttcacc
    18781 acaagaagca acgacaagag cagttcttga agagcgcata cggctgatac cagatacttt
    18841 tttagtggca cttatcgatc aggaaattgt tggctatatc gaaggtccag ttgtcactac
    18901 tcctattttg gaggattctt tgttccacgg ggtgactaaa aatcctaaaa ctggaggcta
    18961 tattgctatt actagtttgt ccatagctaa acattttcag caacaagggg ttgggacagc
    19021 tttgttggcg gcactaaaag atttagtagt agcgcagcaa agaacaggtc ttattttgac
    19081 ttgtcatgat tacttgattt cctattatga aatgaatggg tttattaacc aaggaatttc
    19141 agagtctcaa catggtggaa ccctttggta ccaaatgatt tggaaacttt agaatcacag
    19201 ctgattcaac tgttggtttg ttaaaaataa gtaacaaatg catcatttaa ttgcaattag
    19261 gatactttta agttataatg gcggttgatt atatttagga ggattggctt tgaccgattt
    19321 taaggataaa gaccaacaag atcagcagcg aagctttaaa gagcagattc ttgctgagtt
    19381 ggaaaaagca aatcaaatca gaaaagaaaa agaggaagaa ctttttcaaa aagagttgga
    19441 agctaaagaa gcagctaaga gaacggccca gctatatgct gaatataaaa ggcaagatgc
    19501 ttttcaaaaa gagtctatag cacataacaa taagacagct aaacactttc aagctataaa
    19561 aggtgcggta atgacttcag aagcgcttaa accgacttta ctttctgaaa aagaaaactc
    19621 atctttaaaa acgacaaata agagagtcgt gcaggcaaat gagcttcaag agactgcctc
    19681 taaagaatct caagtaccgt taactattga gaaaggtcat tcagtgagac gaaaattaag
    19741 caaacgccaa cagactgagc gagctgctaa aaagatttca accgttttga ttagttctat
    19801 tattataacc cttttggctg ttactctagc aggagcaggc tatgtttata gtgctttaaa
    19861 tcctgttgat aaaaatagtg atgcctttgt tcaagttgag ataccatctg ggtcaggcaa
    19921 taaattgatt ggtcaaattc ttcaaaaaaa aggtttaatc aagaatagca ctgtttttag
    19981 tttttataca aaatttaaaa actttacaaa ttttcagagc gggtattaca atctgcaaaa
    20041 aagtatgagt ctagaagaaa ttgctagtgc tttacaagaa ggtggtacag cagaacctac
    20101 caagccatct cttgggaaga tcttgattcc agaaggatac acgattaaac aaatagctaa
    20161 agctgttgag cataatagca agggaaagac caaaaaagct aaaacacctt ttaacgagaa
    20221 ggattttttg gatttagtca cagatgaggc ttttattcaa gatatggtaa aaagatatcc
    20281 aaaattatta gcaactatcc caactaaaga aaaagctatt taccgtttgg aaggttacct
    20341 tttcccagca acctataact attacaaaga aactaccatg agagaacttg tagaggacat
    20401 gctggcagct atggatgcta ctttggtacc ctattatgat aaaattgctg ctagtggtaa
    20461 gacagtcaac gaggtattga ccttggcctc tttggttgaa aaagaaggtt caacagacga
    20521 tgacagacgt caaattgcaa gtgtctttta taaccgcctt aatagcggaa tggcactaca
    20581 atctaatata gctattttgt atgcgatggg gaaacttggt gagaaaacaa ccttggctga
    20641 ggatgctact attgacacca ccattaattc tccttataat atttatacca atacaggtct
    20701 gatgcctggt ccagttgcta gctcgggggt ttctgcaatt gaagcaaccc taaatccagc
    20761 ctcaacggat tatttatact ttgtggccaa tgtccatact ggtgaagttt actatgcaaa
    20821 aacatttgaa gaacactctg caaatgttga aaaatatgtg aatagtcaaa ttcagtaaac
    20881 acaaacatgg tgcttaagca ccatgtttgt gtttctgatt cgttcaaaaa agaaaagaga
    20941 gaaaacaatt atggcagaaa aaacgtatcc gatgacccta acggaaaaag aacaacttga
    21001 aaaagaactt gaagaattaa aacttgtgcg tcgtccagaa atagtggagc gtattaaaat
    21061 tgcacgttcg tatggagatc tttcagagaa ctctgaatac gatgccgcaa aagatgagca
    21121 agcttttgtt gaaggtcaaa tttcaacgct tgaaactaag attcgctatg ctgaaatcat
    21181 tgacagtgat gctgttgcaa aagatgaagt cgctatcgga aaaacagtta tcgttcaaga
    21241 agttggaaca acagataaag atacctatca tattgtaggt gctgctggag cagatatctt
    21301 ctcaggaaaa atttcaaatg aaagcccgat tgctcaagct ttgattggta aaaaaacagg
    21361 tgacaaggtt agaattgaat caccagcagc tacttatgat gttgaaatca tcagcgttga
    21421 aaagacaaat taatcagatg aagccattat ttgcctaacc cctaagtgtt ttggttgcta
    21481 atggcttttc tagtatcttc aaatgaggtt gactgttagg aaggtcaagt attaatcgca
    21541 ttgattatga gccaagagga ctatagatca aagatcgttt tcgatggtta tttaatacca
    21601 aaagaaaaga ccagcacctt aatgtactga ccccaaaaag ttggacacta tattttaagt
    21661 ataggaagtg tataatctaa ggaaactgac actaatgatt gaccgttttg caagacttta
    21721 ttaatgattt cttgctttat tttaaatgaa taagtctgat acgtttcctt ccttaacact
    21781 atcgactcca taatgaccca tcagtcaaac ggtatatctc aaacgactac ggtttactcc
    21841 aaattgattg gctagttgag gccaagaaac acctgatttt tttaatgcta aatttctagc
    21901 ttatcttcat agcttaattt catacaaaaa caccccaatc gttagatttt tttgatatgt
    21961 cccctttcac tagatgagtc cagtaaaagg gggcatatcg ttttttgtct aacttttgga
    22021 gtgcagttca taattgtgct aggttttttg tctttcttaa cgctttcgct gttttccagc
    22081 attacgattg gattttattt ttttaggcaa gttagcctgt tccatgtttt gtgaaggcgt
    22141 aacatcctta cgagaagaag ttgattggta tgctttaggc ggggttttag cgtattcttc
    22201 tttaatttgc ttatgcaatc gaggttttaa gaggtaggtt gtgattaact gttgaataat
    22261 actaaagaaa ccaccaacta gccagtaaag accaacacca gcagggagtg agaaggacat
    22321 gaagatcatc atgataggca tggtgtacat catcgtcttc atttgctcac gttgttcttc
    22381 tgaaacggcc atcattgata accatgattg gaagaagtaa agagcagcga taattgctgt
    22441 taacactaaa ctacggctac caagatcaat gcccataaag gtacttgttg atactccttt
    22501 agtgtattga gcggcaaaat acatggcaga aaagaatggc atctggatga gaagaggtag
    22561 acatccaata cctccaagag ggttaatccc atgagcacgc tgagcagcca tcaattctgt
    22621 ttgggcagcc attttttctt cctgactact tgcctgtttg atacgtttgt tgattggttc
    22681 aaaaacaggt ttcaagaagg tcattttctc agattgataa cttgctttcc aagattgata
    22741 tagacctagt ggtaagataa gtgttctaac aataatggtt acgatgatga tagccagtcc
    22801 ataacctagt ccagcattgt ttgcaaagta atcgataaag tatgacatgg gttttccaag
    22861 aaattcccaa atcatacctt tgggatttcc gtgagcgtct cttcccacac agcctgtcaa
    22921 ggtgaataga attgacagtg ctagtcctga aaagaggatg cgatttaatg ttagtttcaa
    22981 tgtgtttatc ctttaatttt aatatactta tctattttac agtttttttc agaaattcac
    23041 aatagttctg aaggccgatt tttaattgcc tatttgaaag tcctgaaagt cttcaaaatt
    23101 agccatggtg acatccacat aagtgacttt tgcccatttg gatggtcctt tacgaacttc
    23161 ttgaataaat gtggctattt tatctgaatc ttttgattgg gccagaattt caacggtccc
    23221 atcgttgtta ttccagacac gaccataaat atctcctata tctagagcta aagtatgggt
    23281 agcgtatcga aaaccaacac cttgtacacg tccagaaaca attaaacgta ctttttgcat
    23341 aagaagggcc tccttgcttt tattatagca catacttagc aggaaatctc tttagctaat
    23401 tgtctacttc ctgtcacttt tactaaagac aagaatacaa aaatgagata gttagtagta
    23461 ctaacagtaa aagagatttg tctattttaa aaatagtatc aaaaaaaggt cttttaggtc
    23521 taaaacattg aactgacccc aaattgacca tctaagaagt tgatgctttc aaatgctgtc
    23581 gcgtatggta taataggcgc atggttatta catcaaaagc aaatcaacta attaagcaaa
    23641 caaaaaaatt attgcaaaaa aagcatcgaa aacagtccta tttgattgag ggctggcacc
    23701 tttttgaaga agcccaaaaa tcaggacaag tttttcgcca catctttgtt ttagaggaaa
    23761 tggtggagcg tctagcaggt gagcaagaac tcgttattgt tagcccacaa gtgctcaagg
    23821 agttgactga ctctcctagt ccacaaggca ttgttgctga ggtggagata cctaagttag
    23881 cctttccatc agattacaaa gggaaatatt tggttttaga ggacgtccaa gacccaggaa
    23941 atctggggac catcattcgt acagcagacg ctgcacggtt tgatggggtc tttctttctg
    24001 aaaaatcagc agatatttac aaccaaaaaa ccctacgttc catgcaaggt agccattttc
    24061 atttaccaat ttggcgaaca gatgtctatc agctttgtcg agagctccaa gagtatgaaa
    24121 ctcccatcct agcaacaact ctgtcaaaga aatctgttga ctataaaagt ctcacacatc
    24181 atgaaaggtt ggctttggtg ttgggaaatg aaggccaggg cattagtgct gaaatggcag
    24241 ccctagcaga ccaactggtt catattacaa tgcctggtca ggcggaaagt ttgaatgttg
    24301 ccgtagcagc aggaatcctc attttcagct tgatttaagc taatatgata taataaccaa
    24361 gtgaggtaat tatgttatgc attatacaga agataaagaa tatatggaac acgtcggtca
    24421 tttgatcgct catcctcgct ttcaaaaatt gagtcatatt gttcagcacc aacattcaac
    24481 gcgtcttgaa cattctatca atgtttctta ttcaagttat aaattagcca agcgatttgg
    24541 ttgggatgct aaaagtacag ccagaggggg cttacttcat gatttctttt attatgattg
    24601 gcgcgtgacc aagtttaata agggtcatgc ctgggttcat cctcgtattg ctgtacgaaa
    24661 tgcgaaaaag ttaacagaac tcaataaaaa agaagaggat atcattctaa aacacatgtg
    24721 gggagcgacc attgcttttc cgcgttacaa ggaaagttat attgtgacaa tggtagataa
    24781 atactgggct gtcaaggaag cagtgacgcc tttgagacaa aaatggtcta atcgacggtt
    24841 tcttcaccgc aagaccctcc aaagtcataa tcgttaagta aaggagttat tatgaacgat
    24901 cacgttattt atacacaatc agatgtggga ttaaatcagt tttttgctaa aatttatagt
    24961 ctagtgggaa tgggagtggg tttgtctgct tttgtttctt atttgatgct ttatccattt
    25021 agagaaaatt taatctctat tcttgttaat cagccgatga tttactatgg tgctgcgatt
    25081 attgaactta ttttagtttt tgtagccagt ggtgcagcac gaaaaaacac accagctgca
    25141 ttgcccattt ttcttatcta ttctgcgcta aatggcttta ccctaagctt tattattgtg
    25201 gcatacgctc aaacgacagt ttttcaagca tttctttctt cagcagctgt gttttttgcc
    25261 atgtcaatca ttggtgtcaa gactaagcgg gatatgtcag gtcttagaaa ggctatgttt
    25321 gctgctttga ttggtgttgt tgtggctagt ctgattaatc tttttattgg tagtgggatg
    25381 atgagttatg tcattagtgt gatttccgtg ctaattttct caggtctcat cgcatcagat
    25441 aatcaaatga ttaagcgcgt ttatcaagca acaaatggcc aggttggtga tggctgggct
    25501 gttgctatgg ccttaagctt gtatttagat tttattaact tatttatcag ccttcttcgt
    25561 atttttggaa gaaatgatta acattaaaaa gtgagtataa ctactcactt tttaatgtta
    25621 acaggataag taaactgtaa ggaagaaatt cgagatagat ataagatgag gctgctaaaa
    25681 gacctagtgt taatttgtaa actctttctt tttttggtat actattaagg attaaatacg
    25741 aggagaatat agatagatat gtcaacagct atttggattt tattacttat tgttgccctt
    25801 ggggtaggtg tttttggtgg gatttttatt gcccgtaaac aaattgaaaa agaaattggt
    25861 gagcacccac gtttaacacc agaagctatc cgtgaaatga tgagccagat ggggcaaaaa
    25921 ccaagtgaag caaaaatcca acaaacttat cgtaatatca tcaaacaatc aaaagcagcc
    25981 gtatccaaag gtaaaaaata aaagctaatt gttatagggg aaacctttgt gatagttaga
    26041 agtgaacagt ctttgatctc aatgttcagt agcacttgtc ttatcagtta gatttgtcaa
    26101 ctgttcaggg tttaaaaaga aactcagttg gctatttcca actgagtttt tcatatcgtc
    26161 agtaaggaag taaaggtaat ggatacaaga ccaattgggt ttttagattc tggtgtggga
    26221 ggtctgaccg tcgtttgtga attgattcgt cagctccctc atgagaagat tgtatatatt
    26281 ggtgattcag caagagcccc ctatggtcct agacccaaaa aacaaattaa ggagtatact
    26341 tgggagctag tcaatttttt gctgactcaa aatgttaaaa tgattgtttt tgcatgcaat
    26401 accgcaacag cggtggcttg ggaagaagta aaagcagctt tagatattcc tgttttaggg
    26461 gttgtcttac cgggggcaag cgcagctatt aaatcaacga caaaaggcca ggttggggtc
    26521 atcggaaccc caatgacagt ggcttcagac atttatcgca aaaaaatcca gctattagca
    26581 ccatctattc aagtaaggag tcttgcttgc ccgaagtttg taccgattgt ggaatcaaat
    26641 gagatgtgtt cgagtatagc taaaaaaata gtttatgaca gtctagcacc attagtcggt
    26701 aaaatagata cccttgtact aggatgtact cactatccct tgttacgacc aattatccaa
    26761 aatgttatgg ggccatctgt taagctgatt gacagtggag cagaatgcgt ccgagatatc
    26821 tctgtcttat taaactattt tgacattaac ggtaattatc atcaaaaagc tgtcaaacac
    26881 cgctttttta caacagcaaa tcctgagatt tttcaagaaa ttgcctcgat ttggctgaaa
    26941 caaaaaataa atgtggagca tgtcacctta tgagtgaaaa aatttacgaa tacaaagatg
    27001 aaaataactg gtttatcgga aagatgactg gtcataattt aatatcgggc tggggagtca
    27061 aacacacgac aatcaaaaaa atagatgacc ttttagacgg tattgccgca acgttagatt
    27121 gggaaaatcc taaaggttac gatgtttcgg tggtgcgtta tcagtctcct ttatctctca
    27181 tcacctttat tatcgatatg attaaccaag aaacccagcg tgagattaag gtgattcccc
    27241 atgcaggcac gattttatta atggaaaatg cgaaactgct tgcagtttat ctcccagaag
    27301 gtggagtttc aacggctaca ttttttgcga ctagtgaaca gggatttggg gatactattt
    27361 taatcgctac acgaaacgag ggaaaaacca aagagttccg caatctgttt ggccagctag
    27421 gttatcgtgt ggaaaatctc aatgattatc cagaattgcc agaagtggca gaaactggta
    27481 ctacttttga agaaaatgct cgcttgaaag cagaaacgat ttcacgcctc acaggaaaaa
    27541 tggtattagc agacgattca ggactaaaag ttgatgcctt gggtggttta cctggggttt
    27601 ggtcagctcg tttctcagga ccagatgcca cagatgccaa gaataatgcc aaactcttac
    27661 acgaattagc tatggtgttt gaccaaaaga agcgctcagc tcaatttcat acgactttgg
    27721 tagtggctgc tcccaataaa gatagtttgg ttgtggaagc tgagtggcca ggctatattg
    27781 ctacgcagcc taagggagaa aatggttttg ggtatgaccc tgtctttatc gtcggagaga
    27841 ctgggcgtca tgctgcagaa ttagaggctg accaaaagaa tcagttgtcg catagaggtc
    27901 aagcagttcg aaaattaatg gaggtctttc cagcatggca agcaaaacaa tcatagtaat
    27961 gagtgactct catggtgacc gagatatcgt tcaagccatt aaagacaagt atcttggtca
    28021 agttgatgct attttccata atggggattc tgaattaaac agctcagatc ctatttgggc
    28081 tggtatttat gtcgttggcg gtaattgtga ctatgataca ggttaccctg atcgtttagt
    28141 aactcaatta ggcacagtta cgattgccca gacacatggt cacttgtacc atattaactt
    28201 cacttgggat aaactggact actttgctca agaagtagca gcagatattt gtctatatgg
    28261 acacctacac cgtccagctg cttggcaagt tgggcaaacc ctctttatga atcccggatc
    28321 agtaacacaa cctcgcggag aaattaacga gaaattatat gctcgtgttg aactaactga
    28381 tactcaaatt aaagttgatt actttacaag agatcataaa ctttacccat cactgtctaa
    28441 ggaatttaaa cgatgatcgc aaaagaattt gaaacatttt taatgagtca tttagataat
    28501 tatcttatcc cggaacaaga cttggctatt tttatcgaca cacataatgc agaccatgtg
    28561 atgttactat tggtgagcaa tggtttttct agagttcctg ttatcacacg ggagaaaaaa
    28621 tatgtgggaa ccattagcat ttcagatatt atgatgtacc aatctaaaag gcagctgaca
    28681 gattgggaaa tgagtcaaac agatattggc gagatggtta ataccaaaat tgaaaccatt
    28741 agtattactt ctagcctaac agaaattatg cacaagttaa ttgatttccc attcttaccg
    28801 gttgtagata gagcgaatcg ctttgttggg attattacgc gaaaatctat attaaaggct
    28861 gtcaacagtc ttttacatga ttttacagat gattacacga ttataaaaaa atgaagagtt
    28921 atattgaacc ttttattgct agcaaggctt tatctcaaaa tagtcaaaaa gcttatcgat
    28981 acgatttgca acagttttgt caactggttg gtgaacgtgt caatcaagac aaactattac
    29041 tttatcaaaa tagcatagct aacctaagtt tatctgcaaa aaaacgcaaa ttatcaacag
    29101 ctaatcaatt cttatattac ttgtatcaaa taaagtattt aaatagctat tttcgattaa
    29161 cagataccat gaaagtgatg cggactgaga agcagcaagc tgctattatt aatactgata
    29221 ttttctatca aaaaacccct tttgtgtggg ggcaattaat cagcttatta atattggagc
    29281 tgggtttaac tccgagtgaa gtagctggta ttgaagtagc taacttggat ttaaattttc
    29341 aaatgttgac gctaaagact aaaaaaggtg tgcgtgtatt acctttgtcg caaatactaa
    29401 tccctttttt agaacaacaa ctcgttggca aagaagtcta tctttttgaa catcgaggca
    29461 tccctttttc aagacaatgg ttttttaatc acctaaaaac ctttgttcga agtattggtt
    29521 atgaaggatt aactgctcaa aaattacgag agcaatttat tttgaaagaa aaactagctg
    29581 gtaaatcgat aatagaactt agcgatattc ttgggctgaa aagccctgtg accttagaaa
    29641 aatactacaa atcataatgg atataaaact taaagatttt gaaggtcctc ttgatttact
    29701 cttgcacttg gtatcacagt acaaagttga catctacgag gttcctattg ttgaggttat
    29761 tgagcagtac cttaattata ttgaaacctt acaggtaatg aaacttgaag tggctggtga
    29821 ctacatgctg atggcaagtc agctcatgtt aatcaaaagt agaaggctat taccaaaggt
    29881 ggtagaacac atcgaagaag aagatttaga acaagatctg ttagaaaaga tcgaagaata
    29941 tagtcgcttt aaagcagtta gtcaagcgtt agcgaagcaa catgaccaaa gggcaaaatg
    30001 gtattcaaaa cctaaacaag aattaatttt tgaagatgct atcttacaag aagataaaac
    30061 ggtgatggat ctatttctag ctttttcaaa cataatggca gctaaaagag cagttttaaa
    30121 aaccaaccat acagtcatcg agcgtgatga ttataagatt gaagacatga tggcttctat
    30181 taaacagcga ctggaaaaag aaaacgttat tagcttatca gcgatttttg aggaatgcca
    30241 aacattaaat gaagttatca gtatattttt agctagtcta gaattgatta agcttcacgt
    30301 ggtatttgta gagcagctct ctaattttgg ggctatcata ctacgaaagg agaagaaatg
    30361 acgtatttgt cacagataga agcgctgcta tttgttgcag gtgaagaagg gttaagtttg
    30421 cgacatctag cgtctatgtt atcattaacg ccaacagctc ttcaacagca attagaaaaa
    30481 ctctctcaaa agtatgaaaa agatcaacat tctagtcttt gcttgataga aacagctaat
    30541 acctacaggc tagtaactaa agaaggtttt gcaggactct tgcgtgctta tgccaaaaca
    30601 ccaatgaacc aaagtttatc tcgtgcgagt ttagaggtct tgtctattgt tgcatacaaa
    30661 caacctatta cacgaataga aattgatgac attcgagggg taaactccag tggagctttg
    30721 agtaaattat tggcatttga tctcattaga gaagctggca aaaaagatgt ggttgggcgt
    30781 cctcatttgt atgcgacaac agattatttt ctggactata tgggaattaa tcatttagac
    30841 gaattgattg aagtatctgc cgttgaacca gcagatgaag agattgctct ttttaggaca
    30901 caagactgag ttttgcttgt catcaaatag caaatggcgt ttacaaaagg atttgtttta
    30961 aggcgtttta gttgaaaaaa agccttaaac tagggacaac aaaaagttgc tcaaaagaaa
    31021 gaagaatata atgcgaatta acaaatacat tgcccatgcg ggtattgcca gtcgtcgtaa
    31081 ggctgaagaa ttgattaagc aaggcttagt aaccttaaat ggtcaggtta ttacggattt
    31141 agcaaccact gtaaaatcgg gagacgtggt tgaaatagaa ggaagtccta tttacaacga
    31201 ggaaaaggtt tactatttgc ttaataaacc tcgtggcgct atctcaagtg tgtcagatga
    31261 caaaggccgt aaaacggtac tggatttact acctcaggtt aaagaacgca tataccctgt
    31321 tggacgattg gattgggaca catcaggggt ccttatttta acgaatgatg gtgattttac
    31381 agatactatg attcatccta gaaatgaaat agacaaggtt tatctggcgc gtgtcaaagg
    31441 tattgcaacg aaagaaaatc ttcgtccgct aactcgagga attgttattg atggtaaaaa
    31501 gaccaaacca gctcgctaca atattgttcg tgtagaagct gataagaatc gatcgattgt
    31561 agaattgact atccatgaag ggcgaaacca tcaggttaaa aaaatgtttg agtctgtggg
    31621 gcttttggtt gataaattat ctcgtacacg ttttgggacc gtcgacttaa agggactaag
    31681 accaggagaa gcaagacgct taaataaaaa agaaataagt caactgcata acttagctaa
    31741 tacgaaaaaa tgatgaaaaa actattaata gtatctgtta aagcttatca aaaatacatt
    31801 tctcctttat ctcctcctag ttgtcgctac aagccaacct gttcagctta tatgttaact
    31861 gctattgaaa aacatggtac taagggaata ctaatgggga tagcaagaat attaagatgt
    31921 cacccttttg ttgctggtgg tgtcgatcct gttccagaag attttagcct aatgagaaat
    31981 aaaaacacgt ctaaaaacgc tgaaaaggca tgactttatc aaaaaatggc ttgacattga
    32041 tagctgaagt gttataatgg aaaacaagca ttcgttggtt taaatcaaac ctgttatgat
    32101 ttaagttagc gagtcaccat caaccacatt gtttgctgag catgactccg agcagtgtgg
    32161 ttttttaaat taaaaagagt aaatgagatt atgacgacaa aagaacttat caacaaaaac
    32221 gacaaagtaa aaaaggctcg caatcatatt gtcctgtttc aaccccaaat tccacaaaat
    32281 actggtaata ttgctagaac atgtgctgca actaatgcac ctttacatat tattaaaccc
    32341 atgggatttc ctattgatga ccgcaaaatg aagcgtgccg gcttagatta ctgggacaag
    32401 ttagaactgc atttttatga tcatttagaa cagtttatca atcaatgtca cggacagctt
    32461 cacctgatta gcaagtttgc agttaataat tattctcaag ctacttatgc tgatggcgat
    32521 tcccattatt tcttgtttgg acgagaagat acaggcctgc cagaagattt tatgcgagaa
    32581 catgctgaaa aagcacttcg tattccgatg aatgatgagc atgtacggag tttaaatgtc
    32641 tcaaatacgg tttgtatggt gatttatgaa gcccttagac aacagggctt tcaagggtta
    32701 gagttaaagc atacttacga acacgataaa ctaaaataat ggacttttta aaagcgtcag
    32761 tttttggcaa catgcgttgt cagtagctga cgcttttaaa aatttagtca aagctattgc
    32821 tatctaggtt taaatctgct atgctagaag tgtcttcagg gcagggtgtg attcccgacc
    32881 ggcggtgact aagtaactag aagtccgcga gcgcaagctg atgtggtgta actccacaac
    32941 cgacagtata gtctggatga gagaagaccg ggctttttgt gtaggcttat tttagctaca
    33001 caataacgac ggaacttctt tcaatttgaa aaaattggag gaatttttta atgtcaaaaa
    33061 cacataaaat gattatgatt ggtatacttt ctgcgatatc atttctattg atgctagtta
    33121 gctttgccat tattccaggc gcagcttttt taaaaattga atttagtatt attcctgttt
    33181 tatttggttt aatgattatg gacttaaaga gtgcttactt gattttgtta ttgaggtcct
    33241 tattgaaact ctttttgaac aatcgtggag ttaatgattt tattggtctc ccaatgaata
    33301 ttatcgctat cgctttattt gtgacggctt ttgccttagt ttggaaccgt caaaaaacgc
    33361 ttagtcaata tgtatttgct agcttactag gcacagggtt attaacgttt ggcatggttg
    33421 ttctcaatta tacttttgcc attcctttat atgctatatt tgcaaatatt gatatcagag
    33481 cttatattgg tgttactaag tatatgatga ctatggttat tccgtttaat cttgttgaag
    33541 ggttgatatt tgcaattacc ttttattttg tgtatattgc aagtaaacca attttagaaa
    33601 gatacttaca ctaatatgat gacaaacaaa caaactcact ttttaatagc ttcttttgca
    33661 ttgctaatct ttgttataat aggctatacg gtcaaatttt ttcctgaacg tttggcactt
    33721 ctagataata ctattcaagc agagattaga ggcaaccttc ctatagtgct gacgcaattc
    33781 tttaggggtg ttactgtatt tggaaatgtc atgacacaag ttttgttagt tattgtgtcg
    33841 gtcctagttt tattctttat gaagtggaaa attgaagcct tgtttatctt atcaaatggt
    33901 gccattgcag catttttgat tacgactctc aagctctttt accaaagacc aagacctgct
    33961 attgagcatt tggtttatgc tggtggctat tcgtttccca gtggccatgc gatgggtagc
    34021 atgctgattt ttggtagcct attgattatt tgttatcaaa ggctacattc aaaactatta
    34081 caatttgtga ctagcatgat ctttataata ctaattttag taattgggtt atctcgtatt
    34141 tatctagggg tacactaccc aagtgatatt ttagcaggct ttgtattagg atttgggatt
    34201 ttgcagttta tttatccttt ttataagcaa aaacgatttg aatggcgatt tcttttgaaa
    34261 caagactaat ttttcttgag aagacatagg gcttatttaa atcatttaga ataacattgg
    34321 caagcatcac cccttgattg ccagtgttat tttagtttta gttagatgat gtgagctgat
    34381 gattagtaaa tacgtttaga taaaaaacga ttaaagtgct atgtaacgtc agaaaagacg
    34441 tgatttttgt tgatctgtaa tcattaagta agctgatgta gaagtagtga attctaatga
    34501 ctgagctatc ttttgcatag atgactatct tgctagtaat gatttgtgct acaatagaaa
    34561 catgaaaaaa agatatcaga cattaaacga gcattaccgt caactatttg gcgcaaaaat
    34621 gtttaaagtt ccgattgatg ccggctttga ttgtcccaat cgtgatggaa cggtagctca
    34681 tggaggatgt actttttgca cagtatcagg ttctggagat gctattgtag caccagatgc
    34741 tccaattaaa gagcaatttt ataaagaaat tgattttatg caccgtaaat ggcctgatgt
    34801 caatcgctat ttggtttatt ttcaaaactt taccaatact catgatacag ttgatgtgat
    34861 tcgcgatcgt tacgaacaag ctattaacga acctggagtt gttggcatta atattggcac
    34921 acggcctgac tgtctaccag atgataccat tgcttactta gcagaattat cagaacgcat
    34981 gcatgtgaca gtggagctag gcttacagac aacttatgaa gaaacttcta ggctaattaa
    35041 cagagcacat tcttatgatt tgtataaaga aacggtgagg cgactaaggc attaccccaa
    35101 cattaatatt gtctcgcact tgattaatgg attgccaaaa gaaactcatg acatgatgct
    35161 tgaaaatgtg agacgctgtg taacagacaa tgacattcaa ggaattaaat tgcatttgct
    35221 gcatttgatg accaatacta gaatgcaaag agattatcat gaaggacgct taaagttgtt
    35281 gagtcaaaag gactatgttt cgattatttg tgaccagcta gagattatcc ctaagcatat
    35341 tgtgattcac cgtattacag gagatgcccc acgcgatatg ttgattgggc ccatgtggag
    35401 tctaaataag tgggaagtat tgaatgccat tgataaagaa atggaaagac gaggaagctt
    35461 tcagggctgt aaagttgact aatttgtctc attttgacgg atttggatag gagaaaagtg
    35521 atgctaaaac gccccattca tctctcgcat gattttttag cggaggttgt ggataagtct
    35581 agtgtggttg ttgatgccac gatgggtaat ggaaatgata ctgccttttt ggcacaatta
    35641 gctaaaaagg tttatgcttt tgatgtgcaa gaacaagcta ttagaaaaac aagtgagcgg
    35701 ttagcgcagc taggattatt gaacgctgag ctgattttag cgggtcatga agcggttgat
    35761 cagtatgtga cagagccggt gcgcgcagcg atttttaacc ttggttattt gcccagcgca
    35821 gataaatcta ttattacctt accaaatacc acactccaag ctttatcaaa actactaacg
    35881 ttattaatgg taggtggacg tattgctatt atggtctatt atgggcatga tggaggcagt
    35941 ttggaaaaag atgctttatt agattttgtc aagcaattag accaaagaaa ggtttctgca
    36001 atgctttatc agcctcttaa tcaggtgaat accccacctt ttttaatcat gttggagaaa
    36061 ttagcagacg tttagccctt atccctgtgt caagtctact agaagttctg cgattatttc
    36121 ggtggaaaaa gaagaaatcc cataggcttt ggctataaat gtgatataat gaagtcacta
    36181 tattattttt ttaggagtta cattaacatg gaagaccctg tgagtcagcc cttagtgatt
    36241 caatttttat tgttagttgt tttaaccttg ttaaatgctt ttttttcagc cagtgaaatg
    36301 gccttagttt ctctcaatcg ttctcgggtg gaacaaaaag cagcagacgg tgataaaaaa
    36361 tacgctcgtt tgttgcgggt tttagaggaa cctaatcatt ttttatcaac gattcaagtt
    36421 gggattacct ttattagttt actatcagga gcaagtttat cagcttcttt gggtaaggtg
    36481 atctcaggtt ggctaggtaa ttcagcgact gcaaggacag ctggtactat catctccttg
    36541 gttttcttga cttatgtctc tattgtttta ggagaattgt atccaaaacg gattgccatg
    36601 aacctcaaag acaagttggc gattgtttca gcccctatta tcattgggtt agggagactg
    36661 gttagtccct ttgtatggct cttatcagct tctactaatt tactgagccg acttacccct
    36721 atgacctttg atgatgcaga tgagcaaatg acacgtgatg aaatcgagta tatgttatca
    36781 aaaagtgagg caacccttga tgctgaagaa attgagatgt tgcaaggagt tttctcactt
    36841 gatgaaatga tggcgcgtga agtcatggtc ccaaggaccg atgctttcat gattgacatt
    36901 aacgatgatc cgcttgaaaa tattcaggaa atcttaaaac aaagtttttc acgcattcct
    36961 gtttatgatg tggataaaga taaaattatc ggtctcatcc acactaagcg tctcttggag
    37021 tcaggtttcc gccagggatt tgatcagatt aacatgcgaa aaatgttaca agaacctctt
    37081 tttgttcccg aaaccatttt tgtagatgat ctcttacgcc agctgcgcaa tacccaaaat
    37141 cagatggcta ttttgctaga tgaatatggt ggtgtggcag gacttgtgac tttggaagac
    37201 ttgcttgaag aaatcgtcgg tgaaatcgat gatgaaaccg ataaagcaga acaatttgtt
    37261 catgagattg gagacaatac ctatattgtt gttggtacta tgactttaaa tgagtttaat
    37321 gactattttg ataccgaact agaatcagat gatgtagata ccattgctgg tttttatttg
    37381 acaggtatcg gaaccattcc aagccaggag caaaaagaag cctacgaaat agataacaaa
    37441 gacaaacatt tagttctaat caacgataaa gtcaaagatg gccgtattac gaaattaaaa
    37501 ttaatcctgt ctaatataga acagattatt gaggaagact aggctccatg cctagtcttt
    37561 ttgatgaaaa gtgttataat gaaaatgtaa aaacgtttac aagtaatgaa ggagatgcaa
    37621 tgactgagaa agattatgaa caagtaacag ggatggtgca ttcgacagaa agttttggtt
    37681 ctgtagatgg tccaggcatt cgttttatca ttttcttgca aggctgtaaa ttacgttgcc
    37741 aatattgcca taatcctgat acttgggaga tggaaaccaa caattcaaaa atcagaacag
    37801 tcaatgatgt cttgaaagaa gcccttcagt acaaacattt ctggggaaaa aaaggaggaa
    37861 ttaccgtatc tggtggtgaa gctatgcttc aaattgattt tatcactgcc ttattcatcg
    37921 aagctaaaaa attaggtatc cataccacac tagacacctg tggctttacc tatagaccga
    37981 cacctgaata ccatcaggtt cttgataact tattggcagt gaccgatttg attttattgg
    38041 acttaaaaga aattgatgaa aaacaacata aaattgtcac gcgtcagcct aataaaaaca
    38101 tattgcaatt tgctaggtat ttatccgaca agcagattcc tgtctggatt cgacatgtct
    38161 tggttccagg cttgactgat attgatgatc atttgacacg attgggtgag tttgtcaaaa
    38221 ccttgaaaaa tgtcgataaa tttgaagttt tgccttacca taccatggga gaattcaagt
    38281 ggcgtgagct aggtattcct tatcagttag aaggtgttaa accaccaact aaggaacgcg
    38341 ttcaaaatgc taaaaatctg atgcagaccg aatcttacac agaatatatg aatcggattc
    38401 atcagtcata agagctgtta tagggttgag gtttcctcaa ctttttaaag tattataagc
    38461 agatcttttg taatcttcct ttgattttag ggggtaattt tgctaaaata aaatgaataa
    38521 acaaaatgag gtagaatgat tatgtcaaaa attttagttt ttggtcatca aaatcctgat
    38581 acggatgcta ttgcatcatc ttatgctttt gattacttat cccaaaaagc gtttggtttg
    38641 gatactgaag tcgtagcttt aggaacacct aacgaagaaa cagcttttgc ccttgattat
    38701 tttggtgttg aggcaccacg tgtggtagag tctgctaaag cacaagggtc tgaacaagtg
    38761 attttgacag accacaatga attccaacaa tccattgcag atatccgtga ggttgaggta
    38821 tatggtgtgg ttgatcacca ccgtgtggct aattttgaaa ctgcaaatcc tttatacatg
    38881 cgtgtggaac cagtaggatc agcctcatct attgtttacc gtatgtttaa agaaaatggt
    38941 atcgaggtgc caaaagcgat tgcagggatg ctcttatctg gtttgatttc agataccttg
    39001 ttgttgaaat ctccaacaac tcatgtgtct gatcaccttg tcgctgaaga attggcagag
    39061 ctagcagaag tgaacttgga agactatgga atggctcttt taaaagcagg tacaaacctt
    39121 gcaagcaaat cagaagtaga attgattggt attgatgcca aaacatttga attaaatggc
    39181 aatgctgtgc gcgtggcaca agtgaacact gttgacattg cagaagtctt agaacgtcaa
    39241 gaagctattg aagcagccat taaagatgcc atggctgcag aaggttattc agactttgtt
    39301 ttgatgatca cggatattgt taactcaaac tctgaaattc tagctattgg agcaaacatg
    39361 gacaaggttg aagcagcctt taacttcacc cttgacaaca atcatgcctt cttagcaggt
    39421 gccgtttctc gtaaaaaaca agtggttcca caattgacag aaagttttgg agcttaatga
    39481 aaagacgctt taaaaggact tggttttttt gaccaagtcc ttttttcact cttctatgct
    39541 ataatagaag gtatgataac aatatttcat tcagataaac tcaccagaca acctttcttt
    39601 caagacctta tcaattatct cgaccagcat gatcacgtta tattacgaga gatcaaaaaa
    39661 gcctttccta atgtgacagg tattgacaag gccatcgaaa gctatgttca agctggctat
    39721 attcgccgtg aaaataagcg ttatggcatc aatcttcctt tggtgagttc tgatcagcag
    39781 ctggccttag acactatgct ttttgtggac acctgttcag ctatgtatga aaatatttta
    39841 gcggttgttt ttgagactca gctaacaaac caaaccaatc acgtgatgat caaggaaaag
    39901 accaacatca cgagaagtca gttgactcta gctaattatt tttaccgtct caaaagaggt
    39961 gagaagccat cagctgagca gatggacttg tatgacctct tgggggatgt gaatcaggaa
    40021 tatgccctta aatatatgac aacttttttg cttaaattca cgcgcaaaga ccttgtgatg
    40081 caaaaacgtc ctgatatatt tgtggaagct ctggttacac ttggttacct aaaacaagtg
    40141 gagcctacaa cataccagct gctaatgacc ttggataaag aaagtttaac ctttatagca
    40201 ccatagaaaa gagctaggaa caaggactgt tcttagcttt ttgagtgctt ttattttgtt
    40261 tttgccatta agtaaagaaa ataaggggct ccgataagcg acactaaaat ccctgtctgg
    40321 atattactgc caacaagata gactcgtccg actgtatctg caacaagaag taggatcatt
    40381 ccgattaaaa tactaatggg tattgttatt ttatgattac tccctaccaa gtatgtacta
    40441 aagtgtccag ctattaagcc gataaaacta atattaccaa ccaaaagaac actcaaagca
    40501 cctaagccag ctgctaagac aagtatcaaa cgacgcttac ggtttaaagg gagtcctagc
    40561 ccaatagcag tattatcagc taatcccata atattaagga aatgggcttg actataagtc
    40621 aatagccaaa aacacaataa taaaggagcg atgacactca gagtaggcca gtcgtctcct
    40681 attaattggc cgctaagcca attgatgacc aaatcgactt tgtagcgatt aatatgacca
    40741 acaagagcaa ccattagact tgaaagtatg gtagtaacag ctacaccagt aagaattaac
    40801 cgtgtaggat caatttgtcc ttgttttgtc aaagatagcc aataaacgct aaaggttgtt
    40861 acaatagcac caagcatggc aaataaaggc aagtaatggc tgatatgagc ttgctcaaag
    40921 aaagaaaagg agaccacaat agccaaagag atacctgctc ctatagtaat acctaataca
    40981 ccagagtcag ctaagggatt tcgtgttaaa cgttgtaata atagccctga taaagcaaga
    41041 gaaccgcctc ctagacaagc agctagaatt cttggcagac gaatgttaat aacgatgaaa
    41101 gatatggcat ggctagattt tcccaaaaac acatggataa ggtccaaaaa agatagatga
    41161 gattctccaa gggacaaagc taatcccata agacttacaa gtagcaggat caggctagta
    41221 tataacctta aaaggtgtcg gtgagatttc aatagcggcc tcctcttctt attaaccata
    41281 aaaagcatgg aaaaccaatt aagctaacca atgccccaag aggggtttca taaggcggat
    41341 tgagattacg acaagcaata tcaacccata ccatgaaact agcgccagaa actgcacaaa
    41401 gcggcaaaag gtatcggtaa tgatgaggtg taaaatgttt cataagatga ggaatgacta
    41461 agccgataaa agaaatgctt ccagcaatgg ctacagctgc tgatgataaa ataagaacca
    41521 aaatcataaa gactgcactg atcaagttgg tcttttgccc aagagctttt gcctgagact
    41581 cacttagact aaggacggtc aggtgataag ataatagctg ggctaaacaa agactaagaa
    41641 taataagagg agcaatatag ccgatcattt gccaattgac cccgacaaga cctcctgctt
    41701 gccagccgat aacagcattt gtcagatgat agtaattggt aataccttga cctaatgctg
    41761 atagcagtat ggaaaccata gctcctgcta agacaaggcg gagctgatgg tagcctttgc
    41821 cagattgata agaaaggcca aagactaagg ttgctgccag gctagaccct aacaacgaaa
    41881 gcagaataat gagggaatag tgcaagtgag gtacaaacgc ataagccagt accaaggcta
    41941 gtcctgctcc agcattaatg cctaaaaggc caggctcagc gataggatta cgtgtgattg
    42001 cttgcatgat agttccagaa acagctaacg cagagccagt taaggtagca ccaaaaagtc
    42061 tcggtaatcg tatagctagt aaaacattgg ctttatggcc gttttgcttt ccaaataaaa
    42121 tagagttaag atcctgatgt gataaagcaa cggctccaaa gcgcagatga cagtaaatag
    42181 caacacataa gctaaaacta agtagcacaa agacaagcca aaagatatgt gacttagtga
    42241 ttatctgcgt tttttttaag caaaaacgct cgagacacaa aaagggatgc ttagttttca
    42301 cttgataaga ttgcctctcg taaagactct aattgatatt ctaaagaaag aggatcagta
    42361 aagtaaaaga cattggcatt gactttgatc acatggtgtt ttttcacagc tggaatggat
    42421 tgccatagct tgctttcata tagagcagaa ccagtcttat catcttctgc agcaacgacg
    42481 acataatcac caatatagtc tggcaagact tcctgcgaca atgacaggta accttgttta
    42541 aaaacttcag tcttcacttt ttcaggagca tcataatgga aagcttgatg gataacctct
    42601 ccgccacgtc cccagtcttt accaaaaagg taaacgtctt tttcataaag ccccataata
    42661 gtaaaggttg ctttgtcacc tgtaacggcc ttgacttctt tttcataagc tgctgtttta
    42721 gttttccaat cttttaacca tttcttggct ttgccttctt tgttaaagat gcggccgaag
    42781 tcagataaca cttgtaaata atcacgtttg cggtattcaa tcgaaataac aggcgcaatt
    42841 tctgccaatt gtttaatatt ttcttctgta gagccaacaa caatgaggtc aggcttaagc
    42901 gttgtaatgg cttctaaatc agttgcagta acttgctttg ctttttttac agtctttgct
    42961 aagatggggt ttttcttgtc ataagaagta accccgacta gattcatatc taattttttg
    43021 aggtaaccag tgtaagttga agccagactg actactcgct taggctgttt aggaatatct
    43081 ccgtaataag tcacaccagc aatttgaggc ataggcgata aactttttga ggcggtgtta
    43141 gaatgattag ttgcttgatt accacaggcg attaaggtaa tagttgtcaa acagagtgtt
    43201 aatagtagag ttaatttttt catgtatgct aattctccta gaataaagtt attgaagctg
    43261 ataagtcagc actataggac agttgtggat aggatcatca accagtaccg gtttgatttg
    43321 gaagatgtct tgaatgatag gtgaagtcat aacatctgct atagtaccag tataatggat
    43381 cttgccatgt ttcatagcaa tcagatgatc cgaatagcgg gctgaaagat tgaggtcgtg
    43441 aagaaccata acaatagtct tgccggcatc cttatttagg gatttaacga gctctaggat
    43501 ttctagttga tgatttaaat ctaaataagt agttggctca tctaaaaaaa tagtatcagt
    43561 gccttgggct aatgccattg caagccaaac cctttgacgt tgccccccag aaagagcatc
    43621 aacaggttga tcagcatagg ccatgacatt ggtggcttgc atagcccaat gaatggcttt
    43681 tttgtcagct ggactaagat ttccaaagta agattgatgg ggaaaacgtc cgtaagaaac
    43741 caattcgtaa acagttatgc cgttagttgc ttcttgaact tgaggtaaaa gagctagttt
    43801 tttagcaacc tctttagttt ctaatgtggc aatgttttgc ccatttaaat acacaactcc
    43861 ctgttttggt ggtaataatc ttgtcagtgc ttttaataaa ctagacttcc cacagccatt
    43921 ggcgccaata atggtcgtaa ttttaccttc aggaatataa aacgataatt tatcgatgat
    43981 ggtacgctgt tcataggcga ttgtgaggtc ttcagcacta attgttgtca tatggttgcc
    44041 tcctttctaa aaagagtcaa agggttgatg aaagtgcaca ttaaagtatt catctgccca
    44101 ttttaaaata tttgagaagt agcttatttt ctcaaaatct tcactattat agcatagaaa
    44161 aaaggcttaa caaagccttt tttgatgtga tttttaggat gtattaaaaa agaaatatta
    44221 gtttagaatg attaaaaaca atagcgagaa tcattctaaa ctaacgtcaa catactctag
    44281 aggtagtgtt tggcgattgc taaatcacca gcataagcta cttgttgtcc tttaacaatt
    44341 tgataagcat ctgcgccttt cccagcgata atcacagcat cttttgctcc ttggcaaaga
    44401 gacatggctt tttgaatagc ttgctcacgg tcactaataa tctctaccgg gcgtgcgata
    44461 tggctggcaa tttcttttga aatatcttct ggatcctcga aattagggtc atcagcagtg
    44521 agaatgacgg tgaggtttgg atgctgatga atcaccctac caaaatcagc tcgtctgctc
    44581 tcgcctttat ttcctggtgc acctaaaatc aacattaatt tgcctgtctg atgttcttcc
    44641 acaacgctga gtaatttttc taagctgtca ccattatggg cataatcaac aaaaacttta
    44701 gcatgatttg tcatagtgag gacttccata cggcctggga cgcgagtctt agcgatgcct
    44761 ttttgaatat cagctaggct agcacccaaa cgaaggcagg caagtccagc tgccatagca
    44821 ttttcttggt taaaatggcc aattaattga atgtcataat ggccagctaa ttgtccttta
    44881 gcttcaaagg agaaagcttg gctagtggtg atctggttgt cagacaaggg accataaaac
    44941 acatgctctt gatccgctac ctgatcagct agaaatgaaa aatgatccat cccgctattg
    45001 atcacaacag ctctgctatt ctccattaaa agacgcttat gatagaaata atcttcaaac
    45061 gttggatgct cgattgggcc aatatgatca ggactaatat ttaggaaaac cccaacatcg
    45121 aacgtaagcc catagacacg gtcaacgagg taagcctgac tagaaacttc cataatcaag
    45181 tgggtcatgc cattagtcac acactcagcc atcatagcaa aaagatccaa gctttcagga
    45241 gttgtaagtt gtgatttgaa aaaggtcttg ccatctaagg tggtattcat ggtagaaaac
    45301 atggcaggtt tataggattc tttcaacatg tggtaggcaa aataagcagc cgtcgtcttt
    45361 cccttagtac ctgtaaaagc tagtagtttg agcttttctt gagggttgcc atagaaagcc
    45421 atggcaatta gactcatagc ttttttgata tcagtgacca aaacaacggg aataccaagc
    45481 tcgtaatcta cctctgaaat atagagttgt aatccgttag tgattgcttc tttaaggtag
    45541 tcagctttga aagttgctcc tttagcaaaa aaaagggtct tgccatctac ttgacggcta
    45601 tcgtaactca agcgctcaaa acttaagcct tgataatggt agtggtatcc gtcggcatca
    45661 agaacctcac gaaaattatg gtcttttttt aaaatgtcta ataattgttc aatggttatc
    45721 atacccttat tttatcctta aagttcttgt tttacaagtc acaagcaaaa gtttataata
    45781 aaactaatag aaactaggaa agaatagata tgtcgacaga aaaaaaacaa ctgacacaag
    45841 aagaattaat ggttcaggga gcagcctggt ccacagctgg taatttcatt agccgacttt
    45901 tgggagtgtt atacattatt ccctggtata tttggatggg gcaatacgcg attcaagcca
    45961 acgctctttt caatatgggg tataatgttt atgcttattt tttattgatt tcaaccacag
    46021 gactcaatgt tgcgattgct aagcaggttg ccaagtacaa ttccatggga caaactgagc
    46081 atagctatca attaatccga agtaccctaa aactcatgct tggtctgggc cttatttttt
    46141 cagtcattat gtatctgggc tctcctttat ttgcgagctt atctggtggt gatgacacac
    46201 ttgttcccat tatgcacagc ctttctttag cagtttttat ttttcctgta atgagtgtca
    46261 ttcgaggtat ctttcaaggc cacaataata tcaagcctta tgcgattagt cagattgctg
    46321 agcaactcat tcgggttatc tggatgcttt tgaccacctt ccttatcatg aaattaggtt
    46381 ctggtgatta cgcttcagca gtgactcagt caacttttgc agcctttatc gggatggtgg
    46441 ctagtatggg tgttctgggg tactatcttt ggaaacaggg acttctagca gctattttta
    46501 gtaagccaga tcatactgtt tccattgata tcaagggctt gttgcttgaa acattaaaag
    46561 agtctattcc gtttatcgta acaggaagtg ccattcaagc ctttcaattg attgatcaat
    46621 ggacttttgt caatactatg actcttttta cagattatag ccgttctcaa ttgcttgttt
    46681 tatttggtta ctttaacgca aacccagcca aaattacaat ggttttaatt gcagtagcag
    46741 catctattgg tggtgtaggc attgccttat taactgaaaa ttatgtcaaa aaagacatga
    46801 aagcagctgc tcgcttaatc attaacaaca ttgaaatgtt agtgatgttt ttgttacccg
    46861 ctcttactgg ggcaattatt ttagcaagac ctctatattc tgttttttac ggagctagcg
    46921 aggatcgtgc cattcacctc tttgtggcgg tcctcttcca aaccttgttg atggcgcttt
    46981 acaccctttt ttcaccgatg cttcaagctc tttttgaaaa tcgaaaagcg atttactact
    47041 ttgcctatgg tatcttgatt aagttagttt tacagatacc gcttatttat ttgctacatg
    47101 cttatggtcc tttactagcg acgacgattg ctttagtggt gccgatttat ttgatgtatc
    47161 gacgcctata tcaggttact cattttaacc gcaaactgtt gcaaaaacgt ttattattaa
    47221 ccttgattga aaccttatta atgggactgg tcgtgtttgt ggccaactgg ctattgggct
    47281 atgcctttaa accgacaggc cgcttgacca gccttcttta tctcctcatt attggtggcg
    47341 tgggaatgac ggtttacacg gcactcacct tgctgacgca tcaactggat aaattaattg
    47401 gtagcaaagc tagtcgcctt cgtcagaaat taggctggca ttaatataat gacaccaaga
    47461 acgatttcgt gagtgtttat cgaaatcgtt tttttcttaa tgatctcgct tagtaaaggt
    47521 agctgataaa aaatcaggat ttcccaagca aactactgat atttttggta taatgataaa
    47581 agtgtaaaaa aatagaagga agtatatcaa catgggaaaa tgtcaagtta tttcgcatcc
    47641 gcttatccaa cacaaacttt caatcttacg tcgtcaaacc acatcaacta aagattttcg
    47701 tgagttggtt aatgaaattg cgatgctgat gggatatgaa gtgtcacgcg accttccctt
    47761 agaagatgtc gacatccaaa cgccagtctc aaaaacggtc caaaaacagt tagctggtaa
    47821 aaaattagct attgttccga ttcttcgtgc tggtattggg atggttgatg gcttgttaag
    47881 tttggtacca gctgcaaaag ttggccatat cggcatgtac cgtaatgaag aaacccttga
    47941 acctgttgaa tacctcgtta aactaccaga agatattaac caacgacaaa ttttccttgt
    48001 tgatccaatg ttggcaacag gtggttcggc tatcttagca gtggattcgt tgaaaaaacg
    48061 tggcgcagca aatattaagt ttgtctgttt agttgcagca ccagaagggg tgaaaaaact
    48121 tcaagaagct catccagata ttgatatctt cacagcagca cttgatgatc atttaaatga
    48181 acatggctat atcgtgccag gcttgggcga tgctggtgat cgtttatttg ggacaaaata
    48241 aaagagacaa ccatcaaata gtagcctttt taggccaatc gttcttgatt ttgttaaaaa
    48301 aatcagagag atttctcttc tttaaaggaa aagcaagttt atgatttgac ctttattgac
    48361 caaaatatta taataaggac aatcagtaaa aaagagatga cctagtgggt tgccgtctct
    48421 cacaatgacg ctactaaaaa cccttgtaga ttggagaatg tattatgatt cctgttgtta
    48481 ttgaacaaac tagccgtgga gaacgttctt atgacattta ttcacgttta ttaaaagacc
    48541 gtattatcat gttaacaggg cctgtcgaag acaacatggc caactctgtc atagcgcagc
    48601 tcttattcct tgacgcgcaa gacaacacta aagatatcta cctatatgtc aacacaccag
    48661 gtggctctgt ctcagcaggc ttggctattg tcgataccat gaactttatc aaggccgatg
    48721 tgcaaactat tgtcatgggt atggcagctt ctatggggac agtcattgct tcatcaggaa
    48781 caaaaggcaa acgctttatg ttaccaaatg cagagtatat gatccaccag ccaatgggtg
    48841 gcacaggcgg tggcacgcaa caaacggata tggctatcgc ggcagaacat ctcttaaaaa
    48901 cacgtcaccg tttagaaaaa atcttagcgc aaaatgctgg aaaaacgatt aaacaaatcc
    48961 ataaagatgc tgagcgtgat tattggatga gtgctgaaga aaccttggct tacggtttca
    49021 ttgatgaaat catggaaaac aacgaactaa aataaagagg atctctgcaa aagtattcta
    49081 gaaacttcta ggatgctttt tctcttagaa aggaaaataa tgatacagcc tactgcatta
    49141 aaacagcttc aagagttaaa acgtcagttc agatcccttt gcaaccaatt atgagatagt
    49201 ttgaggatga tccttagcag ctgtgtgcaa gaagttatgg cgatttcata aaggatgacc
    49261 tagccagtct ttcttcgtct cttttactta aagtgtggta taatcagatt atgctagcca
    49321 caactaaaaa aggcattgcc attaaaggtg ttgtagatcg gcttatccta gatctcataa
    49381 caatgctgta gtaccgccct taccttgcta ctaaagacgt tgttgaagca caaattgact
    49441 tgcaaaggat cttgactgat aggtacatgt ccggtggaat cgccaaagca gcaaaaagac
    49501 caagaccctt atttggtctc taccaaaata taatagaaaa ggatttagaa tgtttcaaaa
    49561 acaagaacgt attggactcg tcgtttacct gtattataac cgagatgcca gaaagttatc
    49621 aaaatttggt gatttatatt accattccaa gcgctctcgt tacctcatta tttatattaa
    49681 taaaaatgat ttagacacaa aattagaaga aatgagacgt ttgaaatgtg ttaaagacat
    49741 tagaccatca gcttttgatg atattgaccg ccaatttgta ggtaaccttc atcgcgacga
    49801 aacaaacaac catcaaaagg gttaccaacc ccctagttac tgattagcgt ttcagattta
    49861 gttagcttat tcaagtaaga aggttttgac agatgctcac actacttcaa gacaatgaga
    49921 caactagagc attagtgaat aactgagttg actgtcactt ctttaataag aacggatagg
    49981 attggtccta aaatagagca ctgcaa
//
LOCUS       DAUGHY010000008        45813 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.008, whole genome shotgun sequence.
ACCESSION   DAUGHY010000008 DAUGHY010000000
VERSION     DAUGHY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45813
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.008"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            226..729
                     /locus_tag="VH752_000437"
     CDS             226..729
                     /locus_tag="VH752_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ8288811.1"
                     /translation="MSAKLMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFVLVLLLWRFALYPLSQQFPKYQLGCQ
                     AFLVALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVVMMLFRR
                     LIGEQKI"
     gene            713..1096
                     /locus_tag="VH752_000438"
     CDS             713..1096
                     /locus_tag="VH752_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEQ8288812.1"
                     /translation="MNKRFKTGFLALVAMLLVACSQGTKQTQTPSVPKADHHVRLVVK
                     EDTNTIDEKVSFGKGDTVLEILKDNYEVKEKDGFITAIDGIEQDTKANKYWLFKVNGK
                     MADKGADQITVKDGDSIEFYQEVFK"
     gene            complement(1142..1621)
                     /locus_tag="VH752_000439"
     CDS             complement(1142..1621)
                     /locus_tag="VH752_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEQ8288813.1"
                     /translation="MPNLYDFTVKAQNGNDVSLAAYKKKVVLIVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFAKIKVNGKD
                     ADPLFTWLKEEKSGPLGKRIEWNFTKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(1614..3413)
                     /gene="pepF"
                     /locus_tag="VH752_000440"
     CDS             complement(1614..3413)
                     /gene="pepF"
                     /locus_tag="VH752_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ8288814.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKTLQDIDLFKRN
                     YEGRLTSVDDFTQALIEIEHIYIQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVSYENFYQNHENAEIREKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYASVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNELNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVMSTAGYLNLKNNPDGAKEWLDFLKSGGSRTPLDTAMLIG
                     ADIATEKPLRDTIQFLSDTVDQIINYTEEMSHA"
     gene            3572..6370
                     /gene="ppc"
                     /locus_tag="VH752_000441"
     CDS             3572..6370
                     /gene="ppc"
                     /locus_tag="VH752_000441"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEQ8288815.1"
                     /translation="MIEETSKEGDGHLPLKKLESSNNQAIIAEEVALLKEMLENITRR
                     MIGDDAFTVIESIMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVD
                     LAYEINYQNNTDTDYLGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTIL
                     ELTTHIHKLLRKYRDAKAGVINLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVM
                     QYYDGSLIQAVTKLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCL
                     SATVQSEVILNYYIDKLAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRR
                     AFHYIQSRLKQTQNQLTNQPAARMSSSVGLNTSAWSSPASLENPILAYDSPVDFKADL
                     KAIEQSLLDNGNSALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQEACVAELLKGAN
                     IVDDYSSLSETEKCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGE
                     EVIKQHIISHTESVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAA
                     YLSHDIVKSWIATNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKI
                     TFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEM
                     LISASINRMVTQMITDPNEIDSFREIMDSIVADSNTIYRKLVFDNPHFYDYFFEASPI
                     KEVSSLNIGSRPAARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQ
                     GNLERLQHMYQTWPFFHSLLSNVDMVLSKSNMNIAFQYAQLAESQDVRDVFYEILDEW
                     QLTKNVILAIQDHDDLLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDE
                     KLIHTTINGIATGLRNSG"
     gene            6510..7814
                     /locus_tag="VH752_000442"
     CDS             6510..7814
                     /locus_tag="VH752_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEQ8288816.1"
                     /translation="MIISRSRGKTMKIDKRHLLNYSILLPYLILSVIGLIMVYSTTSV
                     SLIQAHANPFKSVINQGVFWIISLVAITFIYKLKLNFLTNTRVLTVVMLGEAFLLIIA
                     RFFTTAIKGAHGWIVIGPVSFQPAEYLKIIMVWYLALTFAKIQKNISLYDYQALTRRK
                     WWPTQWNDLRDWRVYSLLMVLLVAAQPDLGNASIIVLTAIIMFSTSGIGYRWFSAILV
                     MITGLSTVFLGTIAVIGVERVAKIPVFGYVAKRFSAFFNPFHDLTDSGHQLANSYYAM
                     SNGGWFGQGLGNSIEKRGYLPEAQTDFVFSVVIEELGLIGAGFILALVFFLILRIMNV
                     GIKAKNPFNAMMALGVGGMMLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVA
                     VGFVLNIDASEKRDDIFKEAELSYRKDTRKENSKVVNIKQFQ"
     gene            8168..9364
                     /gene="tuf"
                     /locus_tag="VH752_000443"
     CDS             8168..9364
                     /gene="tuf"
                     /locus_tag="VH752_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEQ8288817.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            9605..10363
                     /locus_tag="VH752_000444"
     CDS             9605..10363
                     /locus_tag="VH752_000444"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEQ8288818.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPDVDGALVGGASLEADSFLALLDFLN"
     gene            complement(10462..11697)
                     /locus_tag="VH752_000445"
     CDS             complement(10462..11697)
                     /locus_tag="VH752_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ8288819.1"
                     /translation="MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKKGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQKDVIELI
                     LKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKF
                     YSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCY
                     PFPIKYHFIRLAKKLLNRY"
     gene            complement(11684..12910)
                     /locus_tag="VH752_000446"
     CDS             complement(11684..12910)
                     /locus_tag="VH752_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HEQ8288820.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKNNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTVGKSKRAL
                     FVKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGVEWSGRTKELEDTIQPRIQANI
                     YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLDIYAGQSYITMASLDLPEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLATGQTRIPLAATLTLIYGETSENLYAGMDDDYRNYQAPLLTWY
                     KTAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            complement(12910..13719)
                     /gene="yidA"
                     /locus_tag="VH752_000447"
     CDS             complement(12910..13719)
                     /gene="yidA"
                     /locus_tag="VH752_000447"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEQ8288821.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHVVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFLSRK
                     LGIHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            complement(14175..15476)
                     /locus_tag="VH752_000448"
     CDS             complement(14175..15476)
                     /locus_tag="VH752_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ8288822.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEALFHTDHEAFTQEIITNPETEINAILVRHAPDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPEQQAYFQKTAPGLIPFFEKKANLAD
                     YIALDDGVMNTYFQVWMASEDHILSDLASRFINRKILKSVTFDQDSQGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMIQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            15559..15945
                     /locus_tag="VH752_000449"
     CDS             15559..15945
                     /locus_tag="VH752_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEQ8288823.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGGYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            16175..18856
                     /locus_tag="VH752_000450"
     CDS             16175..18856
                     /locus_tag="VH752_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEQ8288824.1"
                     /translation="MSKEQRHEAFYTQSEETVLAQLETSREGLTSAQAKERLAEYGRN
                     ELDEGEKRSLFMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKSMSSPLACIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVIT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQH
                     PLEGLMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDP
                     TETALVQFGLDHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPD
                     QLLKRVTQIEENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEI
                     VEANLVFSGLVGMIDPERPEAAQAVKVAKEAGIRPIMITGDHQDTAKAIAKRLGIIEE
                     DGVDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVGMTGDG
                     VNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTI
                     QYLLSANMAEVFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHK
                     PRGRKSSFFDGGVKEAILYQGAFQTILVLGVYGFALMFPEHTSYHDVHADALTMAYVT
                     LGLIQLVHAYNVKSVYQSIFTVGLFKNKLFNYSIPVAFVALMATVVVPGFNQFFHVTH
                     LTITQWLVVIIGSLLMVVLVELVKAVQRSLGQDEKAI"
     gene            complement(18940..19935)
                     /locus_tag="VH752_000451"
     CDS             complement(18940..19935)
                     /locus_tag="VH752_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ8288825.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGKFDKMSEETKNRIKSTI
                     EATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(19999..21906)
                     /locus_tag="VH752_000452"
     CDS             complement(19999..21906)
                     /locus_tag="VH752_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEQ8288826.1"
                     /translation="MSLAFARFKETVNPDFCRNYLLDYQTDSYADQKRIADLLLTNTF
                     LFEDNWDMEPCHIPYHLDPITWQEAVTDDPEWNFMLNRQTYLQTLILVYLVERDERYL
                     LKAKSFILNWIESAIPLDPKGLATRTLDTGIRCFAWVKCLIYLNLFDALTKQEERLIL
                     ASLEKQLHFLHANYLDKYSLSNWGILQTTAILLADAYFGSNLDIAAATAFARKELTQQ
                     IALQILEDGSQFEQSTMYHVEVLKALLELTALVPDYLPQLRPTLLAMSDYLLKMTGPD
                     HKQIPLGDSDFTDTRDILTLAATILEEPHLKAAAFPTLDIDSLLLLGEKGVHIFEQLP
                     VQTLPTFAHHFEHSGHITINQENYYLFFKNGPIGSSHTHSDQNSICLYYKGQPLFCDA
                     GRYTYKEEPLRYALKSASHHSTAFLEEQLPEQIDSSWAYLSYPKSNYCHLRQNGHIYF
                     IDGSYQTQFSDRNNYQHDRQILILPPGIFLIIDTIQAQGNHCLVSQFILDNHLEVKTD
                     HLSDLRLISDCPFTIEETILSKKYNQYLTSHKLIKRKPFKDKGCTSTLLVPDDTKVTP
                     LTPLQTGKRNPIETALSWHLKGKQFDYSICVLQEDLIKGEKLVLLNGHKIRGKVVVIN
                     HITNEIIRLKH"
     gene            complement(21993..22814)
                     /locus_tag="VH752_000453"
     CDS             complement(21993..22814)
                     /locus_tag="VH752_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ8288827.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYSNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFLPFITSLHLVMLENDRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPIFFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAVVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVLLTVLIGIIGSWLGILA"
     gene            complement(22801..23583)
                     /locus_tag="VH752_000454"
     CDS             complement(22801..23583)
                     /locus_tag="VH752_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ8288828.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGIILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVVIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWAFAFIGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            complement(23602..24090)
                     /locus_tag="VH752_000455"
     CDS             complement(23602..24090)
                     /locus_tag="VH752_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ8288829.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKFLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQNIFIVVKDLQDAKRLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAIRCLAHDHHVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            complement(24126..25325)
                     /locus_tag="VH752_000456"
     CDS             complement(24126..25325)
                     /locus_tag="VH752_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEQ8288830.1"
                     /translation="MTRSLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYSGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTPEAREAALKAADKLVQRYQDKGGFIQ
                     AWGELGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDASA
                     YHTFYFDPETGDPVKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFKGM
                     THYFLNRLPKDQVSYWDLIFRDGSGQSRDSSATAIAVCGIHEMLKTLPDHDPDKKTYE
                     AAMHSMLRALIKDYANKNLKPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLRFY
                     KDWNPYW"
     gene            complement(25325..25762)
                     /locus_tag="VH752_000457"
     CDS             complement(25325..25762)
                     /locus_tag="VH752_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ8288831.1"
                     /translation="MIAIIVMGHGHFASGIVSSLELIAGPQEKVTAIDFTAEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVSGLITCSKEGIVDWKTLSQQEDGATDDELGGI"
     gene            26087..26881
                     /locus_tag="VH752_000458"
     CDS             26087..26881
                     /locus_tag="VH752_000458"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEQ8288832.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDKGLAAYRELGIEAHGYVCDVTDEAGIQQMVSQIEDEVGVIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKVVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQADGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            26906..27547
                     /locus_tag="VH752_000459"
     CDS             26906..27547
                     /locus_tag="VH752_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEQ8288833.1"
                     /translation="MKIALINENSQAAKNGIIYDALTSVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFAKGFGWGAELNLTLIFERLFAEPMGGGYPKERAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFKEYFFANAEPSELVAYLKSVLEQ"
     gene            27576..28577
                     /locus_tag="VH752_000460"
     CDS             27576..28577
                     /locus_tag="VH752_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEQ8288834.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACDAQLFFGGSEVNIARTLG
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMTLTAWQGHRVGLYYLENGFGCRASQV
                     YYDRCGSSFSALDKDSLDLAAIFEGIRHFHFSGISLALGKKTQDLIEILAREAKKRDI
                     CISFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRNKADTTTI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNRFKAYAYDGQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGNEKQRNLDFAVATASLKCTVAEDQLYHSVQQVEAVLHNQRDVQR"
     gene            28582..29217
                     /locus_tag="VH752_000461"
     CDS             28582..29217
                     /locus_tag="VH752_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEQ8288835.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQFIVGPNFNRAVA
                     LICHRYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGMSFIRAIKSPLPQVEVMV
                     TGGVSSDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            29514..30164
                     /locus_tag="VH752_000462"
     CDS             29514..30164
                     /locus_tag="VH752_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEQ8288836.1"
                     /translation="MIKGIIFDMDGVLFDTEPFYLRRREDFFKTKGIPIDHLNSKDFI
                     GGNLQELWKELLGKNRDDAIVKAITTDYDAYKQAHKPPYQKLLITEVNSCLEQLKKQG
                     IKLAVASNSKRQDVLLALETTQIKDYFEIILAREDVSRGKPYPDIYNKAVQKLGLQKK
                     QLLVVEDSQKGIAAAKAANLTVFAITDYRYGIDQSQADHKIDHLGQLCVKIGCFGS"
     gene            30861..31985
                     /gene="queG"
                     /locus_tag="VH752_000463"
     CDS             30861..31985
                     /gene="queG"
                     /locus_tag="VH752_000463"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEQ8288837.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRISPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPSSWGFDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCIDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPPATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILAFMSHLTLKDEDSQKELELIRHKWQF"
     gene            32054..33152
                     /gene="prfB"
                     /locus_tag="VH752_000464"
     CDS             join(32054..32125,32127..33152)
                     /gene="prfB"
                     /locus_tag="VH752_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEQ8288838.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            33171..33863
                     /gene="ftsE"
                     /locus_tag="VH752_000465"
     CDS             33171..33863
                     /gene="ftsE"
                     /locus_tag="VH752_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEQ8288839.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            33856..34785
                     /locus_tag="VH752_000466"
     CDS             33856..34785
                     /locus_tag="VH752_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8288840.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(35094..35792)
                     /locus_tag="VH752_000467"
     CDS             complement(35094..35792)
                     /locus_tag="VH752_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ8288841.1"
                     /translation="MPFIFRYSFFNKVLIFWYTILMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIITVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSLGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPS
                     TTICHEKNANPFFH"
     gene            35960..36724
                     /locus_tag="VH752_000468"
     CDS             35960..36724
                     /locus_tag="VH752_000468"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEQ8288842.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGILDYNEETAQAAVD
                     KLSPEDTVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            36832..39333
                     /locus_tag="VH752_000469"
     CDS             36832..39333
                     /locus_tag="VH752_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEQ8288843.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEALFLEGYELTIPRVDTVELAQLFFPRFEKYNLGHLSRQLNIDL
                     AEAHTAIADARATAILFLRLLQKIESLPIECLESLLVYSDSLLFETAMVIQEGLAKAK
                     PYDPNKYIKIRQILLPKGSKALKPYQISKSFPINMALLGLEERPKQTQFAQLIDEDYH
                     QGVASFIEAQTGIGKTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFH
                     IACHSLKGPANYLKLDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQR
                     FAAYFEQLKHDGDIKQSSEFYDYDFWRVSYEKAKTARLLITNHAYFLHRIQDDKDFTR
                     NKVLVFDEAQKLMLQLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQ
                     VSSDYYQNKEHQLAHDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRV
                     TYLNSASKAFIHFQQLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQ
                     DQLVLVDQEAPIVTEVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQ
                     WQVPHLAQEKNGTAYNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDN
                     PEDFFVKKMSHYLLEKGKNPFRDYFLPMTILRLKQAIGRTMRRKDQKSVVIILDRRLL
                     TKSYGQVILEGLGQEFLISQQNFHDCLVETDCFLI"
     gene            39668..40861
                     /locus_tag="VH752_000470"
     CDS             39668..40861
                     /locus_tag="VH752_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ8288844.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGHDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNITYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            40882..42228
                     /gene="asnS"
                     /locus_tag="VH752_000471"
     CDS             40882..42228
                     /gene="asnS"
                     /locus_tag="VH752_000471"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEQ8288845.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVTNKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            42643..43533
                     /gene="rapZ"
                     /locus_tag="VH752_000472"
     CDS             42643..43533
                     /gene="rapZ"
                     /locus_tag="VH752_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEQ8288846.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQPSFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYQVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIVPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            43530..44507
                     /locus_tag="VH752_000473"
     CDS             43530..44507
                     /locus_tag="VH752_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEQ8288847.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLAPPGDLRNVLLAMSDMPKFYERVFQYRFNESDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYPGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCRAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            44504..45415
                     /gene="whiA"
                     /locus_tag="VH752_000474"
     CDS             44504..45415
                     /gene="whiA"
                     /locus_tag="VH752_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEQ8288848.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSLIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFGLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYVQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
BASE COUNT        13778 a         8143 c         9720 g        14172 t
ORIGIN      
        1 aatgagatcc tctctaacta tgtcaagttt atgtcactat ctcttatagt tggtagctat
       61 attctttact atattagttt agtctttgac tatatcatga cagtcagcaa gtactgtttt
      121 gttatttaat tctcatgtta ttatcatttg ataaataagt gagcttcaaa ctggtaaacg
      181 ctctgtaaat ctgtcataat agagaaaaaa acgaggaaga agtttatgtc agctaagctt
      241 atgagtcgca ttgctatcat gtcagcccta tgtgttgtgt tgagaatggt ttttagcagc
      301 ctgcctaacg tgcagcctgt gacggcattt ttactcagtt atcttcttta ctttgggtta
      361 gccgaggctg tattagttat gatgttgtgc ttatttttat cagccttttt gctgggattt
      421 ggtccttggg tcttttggca agttacttgt tttgtgcttg ttcttttatt atggcgtttt
      481 gccttgtacc ctctgtctca gcaattcccc aaatatcagc ttggctgtca ggctttcttg
      541 gtcgccttat gtggcctcct ttatggggtg ttgatagata cttgttttgc gtatttgtat
      601 agtatgcctt ggtggagcta tgttctagct gggatgcctt ttaatatagc gcatgcttta
      661 tcaactctcg tcttttttcc agttgtgatg atgttattta ggaggttaat cggtgaacaa
      721 aagatttaaa acaggatttc tagcgcttgt tgctatgcta cttgttgctt gctcgcaagg
      781 aacaaaacaa acacagactc caagcgttcc aaaagcagac catcacgttc gcttagttgt
      841 caaagaagat accaatacca ttgatgaaaa agtttctttt ggaaaaggag atacggtgct
      901 tgagattctc aaagataatt atgaggttaa agaaaaagat ggttttatca cagcaattga
      961 tggtatcgag caagacacga aagctaacaa atactggctt tttaaggtca atggaaagat
     1021 ggcagataag ggtgccgatc aaattaccgt taaagatggt gatagcattg aattttatca
     1081 agaagttttt aaatagtaac tataatataa aatagacttg gcacgccaag tctattttat
     1141 attaaccaag aagtgcttta aggtcttctt caattagttt aggatctgtc tttgaggaat
     1201 agcgtttaat gacttgacca ttttggtcaa tgagaaattt agtgaaattc cattcgatgc
     1261 gtttcccaag gggaccactt ttttcttctt ttaaccaggt gaacaaagga tcagcgtctt
     1321 taccattcac tttgatttta gcaaagcgag ggaaagtagt gtgataggtc agactgcaaa
     1381 agtgattaat ctcctcagca tctcctggag cttgatttaa aaattggttg caaggaaaat
     1441 ctaagacctc aaatcccttg tcatggtatg tgtcatatag tgcttgcagg gcctggtatt
     1501 gtggggttaa tccacacttg gtagctgtgt taacaatcaa aacaaccttt tttttataag
     1561 cagctaagga aacgtcattc ccgttttgag ccttaaccgt aaaatcatag agattaggca
     1621 tgtgacatct cctcggtgta attgataatt tggtcaacgg tatcactcaa gaattgaatg
     1681 gtatctcgaa gaggcttttc ggtggcaatg tctgccccaa tgagcattgc agtatcaaga
     1741 ggcgtacggc tgccacctga ttttaggaag tcaagccatt cttttgcccc atctgggttg
     1801 tttttaaggt taaggtaacc agctgttgac ataacaagcc cagcagaata ggtataacta
     1861 tataagccca tgtagtaatg cgcttggcgc atccaggtaa gagctgcgtc atcgtcaatg
     1921 tcaacggcat ctccccagaa gtctgttaag acctctttca tcattgcatt taattggtca
     1981 gctccaaagg taccgccttc ttcaatcagg gtgtaaacct tacgttggaa agccgcttcc
     2041 aataggtgag tgatgaagtt atgaaagtaa gtatctgtta aacggtgagc aagagcaaaa
     2101 cgtttttgac gagggtcatc aaactgatgt tccaagtaat cactaagcat caattcatta
     2161 aaggtcgatg gcgcttcgac gtaataagtt gacatatggg tgttgaagta actttggtga
     2221 ttatctgaaa aaatgaattg accagagtga ccgatttcat gaataagggt ataaacatca
     2281 gacattctgc cagtccaact cattaagaca tacggatgaa ccttgtaggg atcagcggca
     2341 tagccaccag aatccttatt ggcgttagca gcaaagtcaa cccagcgttc tgtctgataa
     2401 cgttcgattt ctttcgtgta ttcttgccct aatggggcta gagatttcat cacgaggtca
     2461 taggcaccat caatgctgac ctctggattg agctcattat caatatctag tttccaatca
     2521 gcaaaagtca ttttctcaag accattgact tgggcgacat gttttaagaa tttctgagca
     2581 accggtccaa attcagtcat gatgagatca atttggcggt cgaacaagct acgatccact
     2641 tcctgttcag ctaggaggta atcaaagaca gaagcgtagc ctttcatatc cgctaacaat
     2701 ttttcagatt taaccttggc taagtaagcc gctgcagctg tattttggtg tttacggaga
     2761 ccttttgaaa aagagcggaa ggctttttca cgaatctcag cattttcgtg attttgatag
     2821 aaattttcgt aagaaacaaa gctattttta taggttttac catccacctc aaaatcctcc
     2881 atgtcaaagt caccagcccg cattttggtg taaatatcat agggcgcatt gataacctcg
     2941 cgtaagttag ccaaagcttt ttctacatca ggacttaaga ggtgttcttt ttgaattttt
     3001 gccatgcgaa tagctgcgct gaaataaggg ttcttttcta atgtatctaa cacgtcaagg
     3061 tcggcattgg ctaaggcggt gtcaaagaag ctaagggcaa cgctagcttt agtcatgaaa
     3121 tcatcgccag cctgagcaat ttgagcaaaa ctttcatctg aaaagtcagt ggtttgaggc
     3181 ataaaggcat aagtaccaat atgactcatt tgaatataaa tatgctcaat ttctattaag
     3241 gcctgagtga aatcgtccac tgatgttaag cggccttcat aattgcgctt aaacaggtca
     3301 atatcttgta aggttttttc aatagcgagt aaaaaatctt ggcggtcttt gtagagcgct
     3361 gttagatccc aaagttcatt ttcagggaat tctgaacgtt ttttcaattc catagttgtc
     3421 tccttaacaa tatagtcaag actagtataa cagaatttta gtggaatatg gagactaaac
     3481 ctatccaaac attctttaaa ataaagcgct tacttttgtt aaaaaacaaa cgttatgaac
     3541 atgatcactt ttatttttgg taaaaagtgg tatgatagaa gaaactagca aagaaggaga
     3601 tggtcatttg ccacttaaaa agttagagag tagtaacaat caagctatca ttgctgaaga
     3661 agttgctctt ttgaaagaaa tgttggaaaa tatcacacga cggatgattg gagatgacgc
     3721 gtttacagtt atcgagtcta ttatggtgtt atctgaaaaa caagattaca tagaattaga
     3781 aaaagttgtc gccaatattt caaatcaaga aatggaagtg atttccaggt atttttctat
     3841 tttacccttg ctaatcaata tttcagaaga tgtcgattta gcctatgaaa ttaattatca
     3901 aaataatacg gatactgatt atttaggaaa attagcccta accatcaagg atcttgctgg
     3961 aaaagataat ggtaaagaca ttttggagca ggttaatgtg gttcctgttt taacagcaca
     4021 tccgacgcaa gtacaacgta aaacgattct ggaattgaca actcacattc ataaacttct
     4081 gcgcaaatac cgtgatgcta aggctggtgt gattaatctt gagaaatggc gtcaggagct
     4141 atatcgttat attgaaatga tcatgcagac agatattatt cgcgagaaaa agcttcaggt
     4201 gaaaaacgag ataaaaaatg tcatgcagta ttacgatggt tctctcattc aagcagtgac
     4261 caagttgaca acagagtaca aaaacttagc ccaaaaacac ggtctagagt tagacaatcc
     4321 caaacccatt accatgggga tgtggattgg tggggaccgt gatgggaacc cctttgtcac
     4381 agctgaaacc ttgtgcttgt cagctacggt tcaaagtgag gttatcctca attactacat
     4441 tgacaaatta gcagcccttt accgaacatt ttcactgtcg tcaaccttgg tacaacccaa
     4501 ctcggaagtg gaacgtttag ccagtttgtc tcaggaccaa tcgatttacc gtggaaatga
     4561 accttatcgc agagcttttc attatattca atcgcgtctt aaacaaacac aaaatcaact
     4621 aactaatcag ccagcagctc gtatgagtag tagtgtggga ctaaacacat ctgcttggtc
     4681 ctcaccagct agtttagaaa atcccatctt agcctatgac tctcctgttg attttaaagc
     4741 tgatctaaag gctattgagc agtcgctact tgataatggc aactccgctt tgattgaggg
     4801 ggatttgcga gaagtcatgc aagctgttga tatttttggc ttcttcttag caagcattga
     4861 catgaggcaa gactccagtg tccaagaagc ttgtgtggca gagttattaa aaggagccaa
     4921 tattgttgac gattatagct ctctttcaga aactgaaaaa tgcgatgttc tcttacaaca
     4981 attaatggag gagccaagaa ccttatcctc ggcggcagtt gctaaatcag acctattaga
     5041 aaaagaactg gctatctata ctacggcacg agaattgaaa gataagcttg gagaagaggt
     5101 tattaagcaa catattattt cacatacaga aagtgtctct gatatgtttg agttggccat
     5161 catgcttaaa gaagtgggat tggttgacca gcagcgggct cgtgtgcaaa ttgttcccct
     5221 ctttgaaacc attgaggatt tagataatgc ccgtgacatc atggcagcct atttaagcca
     5281 tgacattgtc aaatcttgga ttgcaacgaa tcgtaattac caagaaatca tgttaggtta
     5341 ttctgacagt aataaagatg gtggctactt ggcatcgggt tggactcttt ataaagctca
     5401 gaatgaatta acggctattg gtgaggaaca tggagtgaaa atcaccttct tccatggtcg
     5461 tggaggaaca gttggtcgag gaggtggccc ttcttatgat gccattactt cacaaccttt
     5521 tggttccatc aaagatcgga ttcgtctgac agagcaaggg gaaattattg aaaacaaata
     5581 cggcaataaa gatgtggctt attaccattt agaaatgttg atttcagctt ctatcaatcg
     5641 tatggtgact caaatgataa cagatcctaa tgagattgat agttttcgag aaatcatgga
     5701 tagcattgtt gctgatagca ataccattta tcgaaaattg gtttttgaca acccccattt
     5761 ctatgattat ttctttgagg caagcccgat taaggaagtt tccagtctca acattgggtc
     5821 aagaccagca gcccgtaaaa cgattacgga aattacaggc ttgcgtgcca ttccttgggt
     5881 cttttcatgg tcacaaaatc gcatcatgtt cccaggttgg tatggggtag gttcagcctt
     5941 taaacgttat attgaccgag cacaaggtaa ccttgaacgc ctccaacaca tgtatcagac
     6001 gtggcctttt ttccattcct tgttatcaaa tgttgatatg gtattatcta aatcaaacat
     6061 gaatattgcc tttcagtatg cacaactagc tgaaagtcag gatgttcgag atgtttttta
     6121 tgagattttg gatgagtggc aattgacaaa aaatgttatt cttgctatcc aagaccatga
     6181 tgatctattg gaggataatc cttcccttaa gcatagcttg aagtcacgct tgccgtattt
     6241 taatgtgttg aactatattc aaattgaatt gattaaacgt tggcgtaata atcaacttga
     6301 cgaaaatgat gaaaaattga tccatacaac tatcaatggc atagcaactg gtttacgtaa
     6361 ttcaggctaa tgctcttgag tattaattgg tatttataga agttttcaag tgactaatca
     6421 gaattttaaa ggttagccct tggagcttct ttttttgagg attcttgact tttccttttg
     6481 tttgatatag aaagatgtta taatgctaaa tgattatctc aagaagtaga gggaaaacta
     6541 tgaagattga taaaaggcac ctcttaaatt attctatatt attgccttat ttaattttat
     6601 cggtgatagg cttaataatg gtttactcaa ccacaagtgt cagcttgatt caagcacatg
     6661 ctaatccatt taaatcggtg attaaccagg gagttttttg gataataagt ctagtggcta
     6721 ttacatttat ctataaatta aaattaaatt ttttaacgaa tacaagggta ttaactgtgg
     6781 ttatgttagg agaggccttt cttttaatta ttgcccgctt ctttacaact gctatcaaag
     6841 gagcgcatgg ttggattgtg atcggaccag tcagctttca gcctgctgag tatttaaaga
     6901 tcattatggt atggtatttg gcattgacct ttgctaagat ccaaaaaaac attagccttt
     6961 atgattatca agcactaact cgtcgaaaat ggtggccaac acaatggaac gatttacggg
     7021 actggcgtgt ttattctttg ttgatggtac ttttggttgc tgctcagcct gacttaggaa
     7081 atgcttccat tattgtattg acagccatta ttatgttttc taccagtggc attggttacc
     7141 gatggttttc ggctatccta gtcatgatta ccggtctatc cacggtcttt cttgggacta
     7201 ttgccgttat tggggtagaa agagttgcta agattcctgt ttttggctat gtcgctaaac
     7261 gttttagtgc cttttttaat ccttttcatg acttgaccga ttcaggtcac caattagcca
     7321 attcttacta tgctatgagt aatggtgggt ggtttggcca agggttagga aattccattg
     7381 aaaagcgtgg ctatttgccc gaagcacaga cagactttgt cttttcagtt gtcattgaag
     7441 agttaggtct aataggagca gggtttattt tggcacttgt tttcttctta attttacgta
     7501 ttatgaatgt tggtattaaa gcaaaaaatc cttttaatgc catgatggct ttaggagtag
     7561 gcggtatgat gctcatgcaa gtctttgtta atattggtgg tatctcaggc ttaattccat
     7621 ctactggtgt taccttccct tttttgtctc aaggtggaaa tagcttactt gtcttatctg
     7681 ttgcagtggg ttttgtacta aatattgatg ctagcgaaaa gcgagatgat atatttaaag
     7741 aagctgaatt atcttatcga aaggacactc gaaaagaaaa tagtaaagtt gtaaatatca
     7801 agcaatttca ataatattct aaaatctcct ttaaggagat tttagaatat agacaatccc
     7861 agtttgatgc tcaaaaaact tgaactgctc tgctgatttc ttatttttgc gtgtgacatg
     7921 agcccaaaca gctgcttttt gctgtggcaa aaataaggtg tggaattcca aaggaagtcg
     7981 ctgctataag caccacctaa ggagagtatc aaaaaagact ttttcttcta tttaaaaaat
     8041 aaagaaatag caaaaaacat aaaatacttg cattttcatt gtaatttgct acaatagtaa
     8101 ggtaaagtta gactgtatgg cctaccgtct atctataaaa tatattttat tggaggcttt
     8161 tcctaaaatg gcaaaagaaa aatacgatcg tagtaaaccc cacgttaaca ttggtacaat
     8221 cggacacgtt gaccatggta aaactacttt aacagctgca atcacaactg tattggcacg
     8281 tcgcttgcct tcatcagtta accaaccaaa agattacgct tctatcgatg ctgctccaga
     8341 agaacgcgaa cgcggaatca ctatcaacac tgcacacgtt gagtacgaaa ctgcaactcg
     8401 tcactatgcg cacatcgacg ctccaggaca cgcggactac gttaaaaaca tgatcactgg
     8461 tgccgctcaa atggacggag ctatccttgt agttgcttca acagatggac caatgccaca
     8521 aacgcgtgag cacatccttc tttcacgtca ggttggtgtt aaacacctta tcgtgttcat
     8581 gaacaaagtt gaccttgttg atgacgaaga gttgcttgaa ttagttgaga tggaaattcg
     8641 tgaccttctt tcagaatacg atttcccagg tgatgacctt cccgttatcc aaggttcagc
     8701 tcttaaagct cttgaaggcg acactaaatt tgaagacatc atcatggaat tgatggatac
     8761 tgttgattca tacattccag aaccagaacg cgacactgac aaaccattgc ttcttccagt
     8821 cgaagacgta ttctcaatta caggtcgtgg tacagttgct tcaggacgta tcgaccgtgg
     8881 tactgttcgt gtcaacgacg aaatcgaaat cgttggtatc aaagaagaaa ctaaaaaagc
     8941 tgttgttact ggtgttgaaa tgttccgtaa acaacttgac gaaggtcttg caggagacaa
     9001 cgtaggtatc cttcttcgtg gtgttcaacg tgacgaaatc gaacgtggtc aagttattgc
     9061 taaaccaggt tcaatcaacc cacacactaa attcaaaggt gaagtatata tcctttctaa
     9121 agacgaaggt ggacgtcaca ctccattctt caacaactac cgtccacaat tctacttccg
     9181 tacaactgac gtaacaggtt caatcgaact tccagcaggt acagaaatgg ttatgcctgg
     9241 tgataacgtg acaatcaacg ttgagttgat ccacccaatc gccgtagaac aaggtactac
     9301 tttctcaatt cgtgaaggtg gacgtactgt tggttcaggt atcgtttcag aaatcgaagc
     9361 ttaattttaa ttaagttccc agaatagcaa tttaagtcag acaacttaag tttgaagaag
     9421 tccccaattc gggggctttt tgctatctta aactccataa aatactgata aataagaatg
     9481 aaaagctttc agaaaaagag tttttatttg tgaaaatatt cctaaaatat aagcaattcc
     9541 ttttaatcgt cggtcatttg tgctaaaata gaagatataa ataaaaaaag aagaggtatg
     9601 tgatatgtca cgtaaaccaa ttattgctgg taactggaaa atgaacaaaa accctcaaga
     9661 agcaaaagca ttcgttgagg ctgtagcaag caaattacct tcaactgacc ttgttgatgt
     9721 tgccgtagca gccccagctg ttgatttggt aaccactatt gaagctgcta aagattcagt
     9781 tcttaaagtt gctgcacaaa actgctactt tgaaaacaca ggggcattca ctggtgaaac
     9841 atcaccaaaa gtgcttgctg aaatgggagc tgactatgtt gtgattggtc actcagaacg
     9901 ccgtgattac ttccatgaaa cagatgaaga cattaataaa aaagccaaag caatctttgc
     9961 taacggttta actccaattg tttgttgtgg tgagtctctt gaaacttatg aagctggtaa
    10021 agccgttgag tttgtaggtg cgcaagtttc agcagcactt gcaggtcttt cagcagagca
    10081 agtagcttca cttgttttag cttatgagcc aatctgggct atcggtactg gtaaatcagc
    10141 tactcaagat gatgctcaaa acatgtgtaa agctgttcgt gatgtggtag cagcagactt
    10201 tgggcaagaa gttgctgata aagttcgcgt tcaatacggt ggttctgtaa aacctgaaaa
    10261 cgttaaagat tacatggctt gtccagatgt tgacggtgcc cttgtaggtg gtgcatcact
    10321 tgaagctgat agcttccttg ctttgcttga tttccttaac taagtcatct aaaaaagaaa
    10381 aggttaaatc cttttctttt ttagatgact taactcttac tataagagaa taaaggtgat
    10441 tctacaggca aatgcattag cctaatagcg gtttaatagc ttttttgcca atctaatgaa
    10501 atgatatttg attggaaatg gataacatct aaatgtccct ggtttttgca aaataaagcc
    10561 attgaaattc tgtttaaagc gcaagacacc gtctgaacca tcaaactttc cagtaatacc
    10621 aagaaaatta tattgtttga tgcccttgtg aattgccttg agcatagcat gctcttgtaa
    10681 tagagctggg ctataaaatt tattaaattt ggggtaagat cctgaaaaga ggtagacggc
    10741 ctcttgttcg gtatagataa aaagacttcc cgcaaggaac acatctttag ttccatactc
    10801 ttcaagaaaa tggagagctt cagaaattct tacctgaaag gtttcgaatt ggctggatag
    10861 ttcttttaat ctattttgct ttttttcaga atgaggattt ttgccaaggt ctcctttaac
    10921 tttgttgatg ctggttgcta gttgtagttg tctttgttta aggttattaa ggtaatcttc
    10981 aaaattaaga gtcgcaacca taaattcaca agagtctcca aaactatcat aaaagtcctg
    11041 atagtaagat aatgatttgt caaggtaatc tctacgatca gaggttgctt cagtgatttc
    11101 tttgaaatgg tgtaactcat ctcgtttcag ttgacgtaat ttaataccaa aagtattagc
    11161 tttttttatt aaagcttttc cttttttgct gaaggaatgg gttaaacgtg aggaatcaat
    11221 tccttctaac tttttaacgt aatgccaaac aggttcaccc tcaggatagc ctgtttttaa
    11281 accgtcatgt ttatatccta acgccgttaa aaggctaata aggtcagtgt ttggatgaga
    11341 aatagggatg ccatcagtat caaatgactg ataattgtcg taaggtttta aaatgagttc
    11401 gataacgtct ttttgtttgg catagtcttt aagttgagtg taaaaatgtt ctaattcttc
    11461 gggatgattg gtattagggc ctgcattgag ttccatacgc caacctccag ccactttttg
    11521 gctaaagacc atagcagcaa cctttagttc accatctttt tcaagtccaa gaaattgtgg
    11581 ttttgcccct tttttagcca tcaagctggc catttctgag gtttggataa aggaatgatg
    11641 gacgagactg tggcagtaat ggtcaaattg ttcttgtgaa atttcaatta gtgccattag
    11701 ttgtgctcct taattttttt cttaagttat aggtcaagat ggctaagctt gaaacaaggc
    11761 cgacaggaat gttgaattca cctgcaaatt cttcgatggt ggggttgagt ctagctttga
    11821 aatgatagag tccaccatct tgttgatttt ccacacctcc taaattatgc cagcggcagc
    11881 ctcttttaaa agcttcttta gccgttttat accaggttaa aagaggagct tgataattgc
    11941 gataatcatc gtccattccc gcataaagat tttcagaagt ctcgccataa atcaaggtta
    12001 gggtggcggc tagtgggatg cgggtttggc ctgtagcgag ctgctcgctt aaaatggtta
    12061 aatctttttg tagccgtgca atggtttttt gattttctgc tactttgcta ggtttactct
    12121 tatctgttaa tcgtgcttgt tctgctaagg ctttgtccaa ctgttggatc aagagttttt
    12181 tttgttcagg taagtctaaa ctagccatag taatgtagga ctgaccagca taaatatcta
    12241 gtaatttttg atagtaggat ttgcctcgaa ggataatgcc ttttcgattc tcggtttttt
    12301 tcatcaaggc tgaaaagtcg tctagcaatt cactgccacc aatcgtgaca ttaacacctt
    12361 tattggtagc ggtacgaatg gattgcttgg cttttttagg caggctgtca aagtcaaaat
    12421 ccttggcgta gatgttggct tgtattcttg gttgaatagt gtcttcgagt tcttttgttc
    12481 tgccagacca ttcgacacca agttttttta gaaaagcaat aatagagagt gtcacatcat
    12541 tttctttgct ctcttttcct tctaaagttt gtttgatgac taaacttgga tcaattttga
    12601 caaaaagcgc tcgtttgctt tttccgacag ttttgagcgt tttaataacg aaatcaagga
    12661 gttcaaaatt ggcgtagtcc atgattggcc cacgtgggat ataaatcata gtgaaaccaa
    12721 gaggaagttt tctgattaaa caagcagcag cagcaacttg taccccattt tcataaaagc
    12781 tgatgcgttc gtgtttccag ttatttttga cttttcccca ctttgaactt tgaagtaagc
    12841 cagcttgtgg ctgtgctaag acaaactgat catgttcttc aggagatatt ccgattttat
    12901 atgtatacat tagtttaata cccacttgcg aatagcatgt gccacaccac tgtcattgtt
    12961 ggatttggtg atgtatttgg ctattttttt caattcagga accccatttt ccatgacaac
    13021 agggttggca acgacttcta acatagcacg atcattttca gcatccccga tggccatggt
    13081 ttgtgacata tctagcccga gttttttagc taagtgtttg atggcattac ccttgctgac
    13141 ggtttttggc atgaactcta aataaaaagg ggttgacttg acgatggtgt acttatcaaa
    13201 gaaatgttga ggaatttgct tgatggccgc gtccaaaagg tcaggttcat caatcatcat
    13261 cattttgatg atttctttat tggtcatttc ttcgggagtg cgatagaaaa taggcatgtt
    13321 taccaaagtc gattcatgaa ctgtgtattt tccaatattc cgattggcag tgtaaatacc
    13381 ttctttggta atggcatgca tgtgaattcc taattttcgg ctcaaaaatt ccgtttccag
    13441 ataatcgtcg taagtcatca attctttaac aatttcttcg ccggtctcgg catcttgcac
    13501 caagccacca ttaaaagtga taacgtggtt tcctttgtgg ttgagttcta attgttcgag
    13561 taatgaaatc actccggcaa taggtctgcc agtagcaatc accacatgaa ccccttgggc
    13621 tttagcctct tggacagctt gaaaaacatc atctgtaatt cgacggtcat cagtgagtaa
    13681 tgtgccatca atatcgacag caaccagttt gatagacatg gcatcctcct tgtttttttg
    13741 tggtttatct taaaaatgtt ctccctccta tcttactaaa aataggtgct tttgtcatgg
    13801 gatgggaaag gaaacttaag gaaaaacgag agaaaacgtt ttagtttagc tcaatcgcct
    13861 ctgtagtaac taaaaagatt gaagaaagta ctctttcttc aatcttagaa tggagacaaa
    13921 cccagtttga tgctcaaaaa gcatgaactg ctctgctgat ttgacaactg atttcttatt
    13981 tctgcgtgtg acgtgagcca aaacagctgc tttttgctgt ggcaaaagtg gtgtcaatac
    14041 tggcagtgac gttacgcgga atttttgaga ccctaggctc aaaaataaag catagaattc
    14101 caaaggaagt cgctgccata agcatcacct aagaaaagta tcaaaaaaga ctttttctgc
    14161 aatcttaaag ggctttactg actaaagtga aaatgcccat tggaaatata actcataaaa
    14221 gtttccttgc taggagcaaa gaggtcatcg agctctaaca tttcctttgg aaaataaaag
    14281 cgacggtctc cgtaggttgt ccctgttaat gctttgacaa taggagataa ctgagataat
    14341 tcagcaaggc tcccgtcttt ttgtatcatt tcaatctgag ttcttggatt ttcgagctct
    14401 ggtcgataaa tatcataagg caggtcgaag ttgatatgga taccggtgta ataatctgga
    14461 tcaaagccaa ctgattcgac caattggcgc aagcgttcta actctccttg ggaatcttgg
    14521 tcaaaggtaa cagacttcag aatttttcga ttaataaagc gactggccaa atctgataag
    14581 atatggtctt cgcttgccat ccagacttgg aagtaagtat tcatgacccc gtcatctagg
    14641 gctatataat ctgctaaatt agccttcttt tcaaagaagg ggatgagtcc aggagctgtt
    14701 ttttggaaat aagcctgttg ctcaggatat aagtgttggg ctcttttgag gagattttgt
    14761 aagatgagct caacagcacg gctggcggga tgaaaataga cctgcatgta catttgaaaa
    14821 cgactgacaa tatagtcctc gacagcatgc ataccactat gttcaaagac aatgccatct
    14881 tcaacaggtc gaatcacacg taggatgcgc attaaatcaa actgaccgta attggcagca
    14941 gaaaaataag aatctcttaa aaggtaatcc atacggtcgc agtcaatctg gctggaaatc
    15001 agttgcacaa cttgcttgtt aggataagta tgattaatca cgctggcaac tttgtctgga
    15061 aaatcaggag catgacgcac caaaatggca ttgatttctg tctcaggatt ggtaattatc
    15121 tcctgagtaa aggcctcgtg atccgtatgg aaaagggcct caaaggtatg agaataggct
    15181 ccatgaccaa tgtcatgtaa gagggcagcc gtcatagtga ccagactttc atctttattc
    15241 cagatatcag cgtatttctc ttcaaaaata gctgtcaccc gacgggcaat ttcgtaaaca
    15301 cctaggcagt gagaaaaacg gctatgttct gcgccgtgga aggtgaaagc tgttgtaggg
    15361 acttgtttga tgcgtcgtaa gcgttggaat tctttggtat tgataaggtc ataaattaat
    15421 ggattatcaa tgtggatata gttgtgaaca gggtcacgaa atactttttc attcataatt
    15481 tttattatat caaaaattga taaaataagg agaaattgat agaataaaca gacgagctta
    15541 gaaatagaaa gaagagttat gaaattacag ttaaccaacc atattcgctt tggagatgag
    15601 acagagatta ttcaagaaat tcatgattgt gagtggcgag aaaaaggggg ctatcagtac
    15661 ctcatctatc aaaacactga caaggaaaag gttgtcatca agtataatga gacagaactg
    15721 accatgagcc gtttttccaa ccctcagtca atcatgaaat tttttgcggg caagaaggtc
    15781 ctgattgctc tacctacacc gatgggggtt caacaatttt tgacagatac cagtcattac
    15841 cacttagatt gttcttgtca aaaattggac cttcattatc atttgcttca agctcaaaca
    15901 gaaatgctat ttgcaagcta tcatcttgag ctgagttggg attaactaaa aagatagcag
    15961 taaatcatct gattagggat tattcttgtt ttttagtcag gtttttacta taatggttat
    16021 tatatcaagg gagtagcaga cggctagccg tgattgtatc gtcaatacgg aatttcggtt
    16081 tccggtacaa tcttaaacag cgagacttgt ttaagaaaat aaacaggtct ttttatttga
    16141 acaactaaaa taaaaagacc aaggaggtac taatttgtct aaagaacaaa gacatgaagc
    16201 attttacacg caaagtgaag agaccgtact ggctcaatta gagaccagtc gcgaaggtct
    16261 tacgtctgcc caagcaaaag agcgcttagc agagtatggt cgtaatgagc ttgatgaagg
    16321 cgaaaaacga agtttattta tgaaattcct tgatcaattt aaggatttga tgattattat
    16381 tttgattgtg gcagcccttc tttcagttct tacagagggg atggaaggtt tgacagatgc
    16441 cattattatc ttggctgttg ttatcctaaa tgctgctttt ggtgtttacc aagaaggaca
    16501 agctgaagct gctattgaag ccttgaaatc aatgtcaagt ccactggctt gtattcgccg
    16561 tgatgggcat gtgactgaga tagactctaa agaattggtc cctggagata ttgtcttgct
    16621 agaagcggga gatgttgtcc ctgctgatct gcgtcttttg gaagctaact ccttaaaaat
    16681 cgaagaagca gcgcttactg gagaatctgt accggttgaa aaagacttgt caacggctgt
    16741 ttcagaagat gccggtattg gtgatcgcgt caatatggga tatcaaaact caaatgtgac
    16801 atatggtcgt gggattgggg ttatcactaa cactggcatg tacaccgagg ttgggcatat
    16861 tgctggtatg cttgctaatg ctgatgagac agatacacca ttgaagcaaa accttgacaa
    16921 cctttctaag attttgactt atgcgatcct tgtgattgca gcggtgactt ttgctgttgg
    16981 agtcttcttg cgtggtcaac acccgcttga aggcttgatg acttcagtag cgcttgccgt
    17041 tgcagctatt ccagagggac ttcctgcgat tgttactgtt gtgctttctc ttggaactca
    17101 agtacttgct aaacgtaacg ccattatccg taaattacca gctgttgaaa cgctaggatc
    17161 aactgaaatc attgcttctg ataaaacggg taccttgaca atgaatcaaa tgaccgttga
    17221 aaaagtttat accaacggga cactccaaag ttcatcagct gatattgctt ttgacaatac
    17281 gacacttcgt gtaatgaact ttgctaatga tactaaagtt gatccttcag ggaagttaat
    17341 tggtgaccca acagaaacag ctttggtaca gtttgggtta gaccacaatt ttgacgtacg
    17401 tgaggctatg gtggcagaac ctcgtgtggc agaattacca tttgattctg accgtaagtt
    17461 gatgtcaacc attcataaac aagctgatgg caaatatttc atcgctgtta aaggtgcgcc
    17521 agaccaattg ttgaagcgtg tcacacagat tgaagaaaat ggacaaattc gtcctattac
    17581 ggatgctgat aaaaaaacca ttcttgatac caataagagt cttgccaaac aagctcttcg
    17641 tgtcttgatg atggcttaca aatattctga tgctcttcca acactggaaa cagaaattgt
    17701 tgaggctaat cttgtcttct ctggtttggt agggatgatt gaccctgaac gtcctgaagc
    17761 agcacaggct gttaaagtag ctaaggaagc tggcattcgt ccaatcatga ttactggtga
    17821 ccaccaagat acagcaaaag ctattgcgaa acgtcttggt attatcgaag aagatggcgt
    17881 tgatcatgtc tttacaggtg ctgaactcaa cgaattatca gatgaagagt tccaaaaagt
    17941 ctttaaacaa tactccgtgt atgcccgtgt atctcctgag cataaagttc gtatcgttaa
    18001 ggcttggcaa aatgaaggca aagttgttgg catgactggc gatggggtta acgatgcacc
    18061 atctcttaaa acggcagata ttggtattgg tatgggaatt acaggtacag aggtttctaa
    18121 gggagcttct gacatggttc ttgccgatga taactttgca accattatcg ttgcggttga
    18181 agaaggacgt aaggtcttct caaatattca aaaaacaatt caatacttac tttctgctaa
    18241 catggcagaa gtctttacca tcttccttgc aaccttgttt ggttgggatg tgcttcaacc
    18301 agttcacttg ctttggatta acttggtaac tgatacccta ccagctattg ctcttggtgt
    18361 tgaacctgct gagcctggtg tgatgaaaca caaacctcgt ggacgtaagt caagcttctt
    18421 tgatggtggg gtgaaggaag ccattcttta ccaaggtgcc ttccaaacca ttttggtgct
    18481 tggggtttat ggttttgcct tgatgttccc agaacacaca agctaccatg atgtccatgc
    18541 agatgctttg accatggctt atgtgaccct cggtttgatt cagttggtac atgcctacaa
    18601 cgtgaaatct gtttaccaat ccatctttac tgttgggttg ttcaaaaaca aattgttcaa
    18661 ctattctatt ccagtggcct ttgtagcatt gatggcaaca gttgttgttc ctggatttaa
    18721 tcaattcttc catgtgacac acttgacgat cacacaatgg ttagtggtta ttatcggaag
    18781 tctcttgatg gttgttcttg ttgaattggt gaaagctgtt caacgatcac ttggtcaaga
    18841 tgaaaaagca atttaataga cgctaaaagg tttgagacaa ttgtctcaaa ccttttttta
    18901 taatcgttac aacgataagg actattggta tgcctgacat taaaaagttg attcttgcca
    18961 attgacttga cagtcaaaaa tttgttgttt ggcttcttga ctgtgacctt caatatcatc
    19021 gattaaaatc ttagtagcct gttcgccttc ttcataggcg ggttggataa tagttgttag
    19081 tgttggtgaa gaaaattttg tccactcaat gttatcaaat cctactagtc caatctctgg
    19141 tatattaaat ttaagagact tcatagctgt aaatactttt ggaagagccc aacaattggg
    19201 gacaaaaacc aaactctttt tagtcagtga tccttgcaaa aactgagcaa ttgcctctga
    19261 agaagtctga ttttcatcaa taatcatttc ttgatgagta agatgattag cttctaacac
    19321 atcaatgaag cctgaagcgc gttcaatacg agtgctaagt aaattggggt tacccgtgat
    19381 catgataaaa tgttcataac ctttatcgat acattgttga atggtatcat aaacagcatc
    19441 ataattatta gttttgaccc agttggtacg atgctcataa agttgactat caaaaaagac
    19501 aaccttcttt tttttaatat cgataatgcg agaatacttc ctgaaattag aggtaggttg
    19561 aatgataaag ccatccactc cgaggttcag catcttttca atgagttcat cttcgcgaga
    19621 agggtcgtag ttgctgtttc cgatgataat ttggtaacca aattcctggg ctttgctttc
    19681 aattccctta acaatttgat tggaaaaact attggtaata tcaccaataa ccacgccaat
    19741 gagtttagta gatttggcgt ttagacttct ggcagcaatg cttggtttat agttggtagc
    19801 ttcaatcgtg cttttaatgc gatttttagt ttcttctgac atcttgtcaa atttaccgtt
    19861 caagtaaaag gaaactgtcg ttttggaagt tttagccaat tcagcaatat ctttgatggt
    19921 tactttgcgc tgcattgcat acactccttt aatttagtaa tggttagtca tattataaca
    19981 taaggactcg taaaagattt agtgtttcaa tcgaataatc tcattcgtga tatggttgat
    20041 aacaaccacc tttcctctga ttttatgacc atttaggaga actagttttt ctcctttaat
    20101 taaatcctct tggagaacac agatactgta gtcaaactgc tttcctttta aatgccagga
    20161 aagggcggtc tcaattgggt tgcgtttgcc tgtttgaagt ggtgttaagg gagtaacttt
    20221 ggtatcgtca gggactaata gggtactggt gcaacccttg tctttaaagg gttttcgttt
    20281 gatgagtttg tgactagtca ggtattggtt gtattttttt gacaaaatcg tttcttcgat
    20341 agtgaaaggg cagtcactga ttagtctgag gtcactaagg tgatctgtct ttacttctaa
    20401 gtgattatca agaataaatt gactgacaag acagtggttg ccttgagcct gtatcgtatc
    20461 aatgataaga aaaattcctg gtggtaagat gagaatttgt cggtcatgtt ggtagttgtt
    20521 cctatcagag aattgcgtct ggtagctacc atcaataaag taaatgtgtc cattttgtcg
    20581 aaggtgacaa taattggatt tggggtaaga aaggtaagcc caggagctat caatctgctc
    20641 aggtaattgt tcttctaaga aggcggtaga atgatggcta gcgcttttta gggcatagcg
    20701 taatggttct tctttgtaag tgtatcgccc agcatcacag aaaagtggtt gtcctttata
    20761 atagagacag atactatttt ggtcgctatg ggtatgggaa ctaccgatgg gcccattttt
    20821 gaaaaaaaga taataatttt cttgattgat ggtaatgtgc ccggaatgtt caaagtgatg
    20881 cgcaaaagtt ggtaaggttt ggacgggtaa ctgttcaaaa atgtggacac ctttttctcc
    20941 tagaaggaga agactatcga tatcaagtgt gggaaaagca gcagctttta agtgtggttc
    21001 ttcaaggata gtggctgcta gtgttagaat atctctggta tcggtaaagt cactgtctcc
    21061 taaggggatc tgtttgtggt cagggccagt cattttgagg agataatcac tcatggcaag
    21121 caaagtaggt ctaagctgtg gaagataatc agggactaga gcagttaatt ccagaagggc
    21181 tttgagtact tccacatgat acatggttga ttgttcaaat tgactgccgt cttcgagaat
    21241 ttgtagtgcg atttgttgtg tcaattcttt tctagcgaaa gctgttgctg cagcaatatc
    21301 aaggttggaa ccaaagtagg catcagctaa taaaatcgca gtggtttgca agattcccca
    21361 attgctgaga ctgtatttat caaggtagtt ggcatggaga aagtgaagtt gtttttccag
    21421 ggaagctagg atgagccttt cctcttgctt ggttaaggcg tcaaaaagat taaggtaaat
    21481 caaacatttg acccaagcaa aacaacggat gccagtatct aaggttctag tagctaaccc
    21541 cttagggtct aaaggaatag ctgattcaat ccaattgagg atgaaacttt tggcttttag
    21601 gagataccgt tcatctcttt caacaaggta gacgaggatt aatgtttgga gataggtctg
    21661 tcgatttaac ataaaattcc actcaggatc atctgtgaca gcctcttgcc aagtaatggg
    21721 atctaagtgg taagggatgt ggcagggctc catatcccaa ttatcttcaa ataaaaaggt
    21781 attggttaag agtaggtcag ctattctttt ttgatcagca tagctgtcag tctgatagtc
    21841 tagcaggtaa ttcctgcaaa agtctggatt gactgtttct ttaaagcgtg cgaaagcaag
    21901 agacatagga cgtttccttt ccaaaagaaa agagacctca tcatgtgctt aggaactatt
    21961 tccctatgct cataacaggt ctctgtgaac cattaagcta aaatacctaa ccagctaccg
    22021 ataataccga taagaacagt gaggagaacc aatttgtagg ttgtccattt tttgttttta
    22081 ataaggtaat acatgagtaa ggtaaagaga gctggtaaaa gtgcaggagc aattttatca
    22141 agcattcctt gaatagtcac aaatttttgt gctgtattgg ccgcatccac cttgccagcg
    22201 gcaaaagtaa tgggaacagt tattttaaca gaagtagcag ctaatccagc gataacggta
    22261 acaccgacaa tatttgccat gcgcgacaca accgccattt gttcacttaa tttatcaata
    22321 aagcttgtgc cgaccttgta gccatagaga ccagaaccga ttttaatagc agttaggata
    22381 atattcatgg ctaggaagaa gaaaattgga cctaaaacta aaccatcact agctaaggaa
    22441 gctgcaatcg ttgaaaagag tggcgccaag caaaattgtg ataaggagtc accgatacca
    22501 gctaaaggcc ccataagtgc cattttgatg tttcgggttt cttcttcagg acgatcattt
    22561 tctaacatga ccaagtgcag actggtgatg aaaggaagga agtgagggtt agtattgtaa
    22621 aactcacagt tatcttctaa agcttgatat aagccctttt tatcactgcc aaaatgtttt
    22681 ttcaaggcag gatagatgac gttggcgtat ccgatacctt ggtaattact gtaattaaac
    22741 ccattttgta ggaagaaagc tctcagagct gttttgaggt aatctttctt ggttaattta
    22801 ttagatccag tcatcttgtt ggcctccttg gacagttgtt gcatcgactt gtttaggttt
    22861 gttgtaaaat tcattcaagg cgaaaatgat accaataata gcgataccaa tggttggaat
    22921 ttggaggtag gctgcacaaa cataaccaat cagtacaaaa ggaatcaatt ctttcttggc
    22981 cataacagat aagatcatgg caaatccgat agcaggtaac attttaccag cgacagtcaa
    23041 tccattgagc aagacaggtg ggatataatc aaccaagtga aggagagtat ccattgacaa
    23101 ggcacctaat aaaccaaggc ctaatccgat aaaagcgaaa gcccagatag tgccgttggc
    23161 agcgaatttg aagcctctaa tattgccttt ttgcaattgc tttttagcgg tttcgggagc
    23221 gccagcaaaa gctgtatagg cgaatgtctg aagaaattgg atgacaactg cgattggtgt
    23281 tgataaggct agggccgctt ctggggtaac tttaccagca ctggtaatag ccatcaaggt
    23341 accaaagata ccagggccga taggattagg aggaacggta ccaccggcac ccactccaaa
    23401 tcccatataa gctaattcgg agatagctcc catgctaagt gcagttggca agtcacctaa
    23461 gataatccct acaccaaaag acagaataat acaacggttg gtgtagatgc caagtaacat
    23521 tccgctgaaa caaaaggctg tccaaagacc aattaaaagt gcctgtaaca aattgatatc
    23581 catatcaatc tcctttattt attaaatata atctaagatg tcaacgtcag aggcgctatt
    23641 gccagctgga gttgttttgg tgttaaaaac cacgtggtgg tcatgggcta agcaacgaat
    23701 agcagacttg tctgtttctc ccagcgaaat aaactgggta atcgcaacct tatcatcagt
    23761 tttatgaata ttaccgatat tgatttctgt gatgggaaca cccccttcaa ctaggcgttt
    23821 ggcatcttgc aaatctttga ccacgatgaa gatattttga gcaggacttg ctttatgaat
    23881 gatgtcaatc actttttgaa tggagaaaaa acgaatggca atactggatg gaataactgt
    23941 tttcatgagg gattgttgaa tcttatcttc agaaaccgca tcgttagcga cgataacggt
    24001 gttgcagttg aggaatttga cccataattg cccctgtccg tgaatcaagc gctcatcaac
    24061 tcgcgtcatg ataatgtttg gttgtgtcat aaaatctcct ttttaaatgt gattatcggt
    24121 gtttgttacc agtaagggtt ccagtctttg tagaaacgaa gcagagcttc aaggtagtaa
    24181 tagtctcccc agatatttcc ttcatccacc ccttttccag agtgccaaga atagactccg
    24241 tgaagcagta gaggagcacc aggcttgaga tttttgttag cataatcctt aatcaaggct
    24301 cttagcatgc tgtgcatggc agcttcatat gtttttttat caggatcatg atctggtaag
    24361 gtttttaaca tttcatgaat accacacaca gcaatggctg ttgctgaact atcacgagac
    24421 tgtccacttc catctctaaa aattaaatcc cagtaggata cctggtcttt aggaagacgg
    24481 ttcaagaaat aatgggtcat gcccttgaag agttgaatga gttcaggttc ttttaggaaa
    24541 cgataggtca aaggaatgcc ataaatgccc caagcttgcc ctctggccca agccgaatca
    24601 tcgctataac cttgtcgggt gaccccttta acaggatccc ctgtttctgg atcaaagtag
    24661 aaggtgtggt aagcagaggc gtcatctcgg atgacatgat tggctgaggc gtagaaatgg
    24721 ttgatcgcca tgtcacggta acggttgtct ccagtttctt ggctagcaaa aaagagtaat
    24781 tggatgttta ggagacaatc aatgattaaa cggtaatctt cttttttccc cagttctccc
    24841 caggcctgaa taaagccgcc tttgtcttga tagcgttgga ctaatttgtc ggctgctttc
    24901 aaagcagctt ctctagcctc aggtgttttt aaaagtttcc actcagccat gcaagaaggg
    24961 gtgtaaagaa agcccaaatc atggtgatct agctcaatgt ctcttgttac tcgatctagg
    25021 aaagaaaggt catttgcttg agccagagct ttaatggcat catccccact gtattcgtaa
    25081 gctaaccaaa ggcaacctgt ccaaaaggca ttggtccatt ccgtgttgtc cataatggcg
    25141 tattggttat ctttggtagc tggcgttgga aagtcttctt taaaatagtc catattaagc
    25201 cgaacttgtt tgagggctaa gtcaagggcc tgagtaatgt cttcttggct tagaaagtct
    25261 tctttggtaa atcgttcagg ctgcttgata ggttcaagag caatggtttt taaagatcgt
    25321 gtcattaaat gcctcctaac tcatcgtctg tcgcaccgtc ttcttgctga gaaagagttt
    25381 tccagtcaac aatgccttct ttagaacatg ttataagtcc tgatactaag tcgtcaaaag
    25441 aagctgctgt ttgtcttgca aaactagctt caatcagcat ggctaagtta aggccagaaa
    25501 gaacattaca cgtcgtgttt ggaagagatt ccattaaggt tgcagcgact ttaaaagggg
    25561 tacctcctag aaggtcacaa agaactaagg tttcctcttc tgggattaaa gcacgagata
    25621 attggtcctg cacatcagcg gcggtcatct cagcagtaaa atcaattgca gttaccttct
    25681 cttgtggtcc tgcaattaac tctagggatg agacaatccc acttgcaaag tgtccatgtc
    25741 ccataacaat aatagcaatc atgataaaac tccttttggt ttaccggttt actcaaaaac
    25801 attgtagcgc tttcaaaatt ctttgtcaat acttttttga gatttatttt tagagaaaat
    25861 agagattaat ctccataaca tgtcaaaagg gatcaatcat agttgtttaa tgaagaacac
    25921 tatgtaaaag aaatgccatt aaggaataaa acctattttt aaaagctagc tagtttctct
    25981 tttttatttt tgaaccgttt tcataaacat cttgacaaaa ctagtttact ttgttatagt
    26041 cttagtaaac aggtttactt aatttaataa caaaaaggag aatcctatgg aaaatatgtt
    26101 ttcgttacaa ggtaagattg ccttgattac cggagcatcc tacggtattg gttttgaaat
    26161 tgccaaagcc tatgctcaag cgggtgcaac tattgtgttt aatgacatca aacaagagtt
    26221 agttgataag gggttggctg cctatcgaga gttagggata gaagcgcatg gctatgtttg
    26281 tgacgtgact gatgaagctg gtatccaaca gatggttagt caaattgaag atgaagtagg
    26341 tgttattgat attctggtca acaatgcagg tattattaga cgtacgccga tgcttgaaat
    26401 ggccgcagaa gattttcgtc aagtcattga tattgattta aatgcacctt ttattgtgtc
    26461 aaaagtagtt ttgccatcga tgattgcaaa aggacatggt aaaattataa atatctgttc
    26521 tatgatgagt gaattgggcc gtgaaactgt tagtgcctat gcggcggcta aaggtggctt
    26581 gaaaatgtta accaaaaata tagcctctga gtttggagaa gccaacattc aatgtaatgg
    26641 gattggtcct ggctacattg caacaccaca aacagctccg ttgagagagc gacaagcaga
    26701 tggtagtcgt catccttttg atcaatttat cattgctaaa acaccagcag cacgttgggg
    26761 gacaacagaa gacttagctg gtccagctgt ttttctagct agcgatgcca gtaattttgt
    26821 gaacggccat attctttatg tcgacggtgg cattttagct tatattggaa aacaacctta
    26881 atcacacact agaaagagat tttatatgaa aattgcatta attaatgaaa atagccaagc
    26941 ggctaaaaat ggtattattt acgatgccct tacctcagtg acagataaac acggctacca
    27001 agtttttaac tatggcatgt atggcacaga aggtgaaagc cagttgacct atgttcaaaa
    27061 tggactctta gcttctattt tattgaccac taaggcggcc gattttgtgg tgactggttg
    27121 tgggacaggt gttggagcta tgttggcctt gaacagcttc ccaggagtta cctgtggttt
    27181 tgcgagtgag ccaacagaag catatctctt ttctcaaatc aatggtggta atgccttgtc
    27241 cattcctttt gccaaaggtt ttggttgggg agcagagttg aatttgacct taatatttga
    27301 acgcctcttt gcagaaccaa tgggtggtgg ttaccctaaa gagcgagcta ttcctgaaca
    27361 gcggaatgca cgtattttaa gtgatttgaa aaaaattact tacagagatt tgttggcaat
    27421 tgtcaaagac atcgaccaag atttcctcaa agaaacgatt tctggcgctc attttaaaga
    27481 gtacttcttt gccaatgcgg agccgtcaga attagtcgct tatttgaaat cagtacttga
    27541 acagtaattc cattttgacg aggaaaggag gtcacatgtc taagttatta ttggttggtg
    27601 aaccgctgat tcgcgttagc cccaatcagt ttcagccttt gaccaatgcc tgcgacgctc
    27661 agcttttttt cggcggttca gaagttaata ttgcaagaac tctaggcggt tttggcttag
    27721 aagcaaggct atttacggct ctaccagata atcctgttgg ccacgctttt catcagtttt
    27781 tgaagcaaag cggtgtggat atgaccttga cggcttggca aggtcatcgc gttgggctat
    27841 attaccttga aaatggcttt ggctgtcggg ctagtcaggt ttattatgac cgctgtggct
    27901 ctagcttttc ggctttagat aaggacagcc ttgatttagc tgccattttt gaaggcattc
    27961 gtcactttca ttttagtgga atcagtttgg cgttgggtaa aaaaactcag gatttgattg
    28021 aaatacttgc gagagaagct aagaaaaggg atatttgtat ttcctttgac cttaacttta
    28081 gatctagtat gattgccgta gctgatgcta aacgactctt ttctcacttt gctcaatacg
    28141 cggatattat ttttggcatg gaacctttgc tgttagacag tgacgatttt gacatgtttg
    28201 ataggaataa ggcagatact acgacgatta gagaacgtct ggcaggactt tatcagcgct
    28261 atcagttaca agctatttac catacggagc gttcaaatga tgcccaaggc agtaatcgtt
    28321 tcaaggctta tgcctatgat gggcagtttt acgaatcctg tgaagtgacg accccagtct
    28381 tgcaacgggt tggaagtgga gatgcttttg tggcgggttt actttatcag ttgttagaag
    28441 gtaacgaaaa gcagcgcaac cttgattttg ctgtggcaac agctagccta aaatgtacag
    28501 tagctgaaga tcagctctat cacagcgtcc aacaagtaga agcagtctta cataatcaac
    28561 gagatgtcca gaggtgatga tatgggaaaa atagaaatct taaccaaact taaggctaat
    28621 cggcttgtcc tggttgtacg aggagagtct agtgaggaag ctttagcttg cagcctggcc
    28681 agtattgaag gtggcattaa gacaattgaa gtcacctata ctaacccctt tgctagtgag
    28741 gttatcggtc aattagctga acgcttcaaa gaagatcctg aggtattgat aggtgctggg
    28801 acagttttgg atgatgtcac tgcccgccaa gctattttag caggagcaca gtttattgtt
    28861 ggtcctaatt tcaatcgagc agtggctttg atttgtcatc gttatagcat accttattta
    28921 ccaggttgta tgacggttaa tgaagtggtt acagcattag aatcaggggt tgatatggtc
    28981 aaaatttttc ctggttctac tgtagggatg tcctttattc gggcaatcaa atctcctttg
    29041 ccacaagtag aggttatggt tacaggcgga gtttcttcag ataatctcaa agactggcta
    29101 gctgctggag tagatgtttt aggcatagga ggcgagttta atcagttagc gagccaaaaa
    29161 caatataacc taatcactaa gaaagccgct cattatatca aaagtttgcg acaataatct
    29221 aaaaaacgtt tttaccttgg ctttagtgct agtaaaaacg cttttttatc attcattcaa
    29281 ttaatatgct tctgcttatg tttttccaga tagtatagat ataagagcag ctaatttaac
    29341 aagatgagcc tgtcgtttga tatataggat ataaactttt tatgatcaaa caaaagtaat
    29401 accttttgaa ttcgttagta ttgacctgat ctaaagtgat atcacttaga atgaactagc
    29461 tgtgcagctt ttacagcaac ttatgctaaa atagaaaaaa aggagaaatg ttgatgatta
    29521 aaggaattat ttttgatatg gacggtgttt tatttgatac agaacctttt tatctgaggc
    29581 gacgagaaga tttttttaag acaaagggaa ttcccattga tcatttgaac tctaaagatt
    29641 ttattggggg caatctccaa gaattatgga aagagttgtt aggtaaaaat agggatgatg
    29701 ctatcgttaa ggcaattaca actgactatg acgcttacaa acaagcgcat aagcctcctt
    29761 atcaaaaact gttgattaca gaagtgaact cttgtcttga acagttgaaa aaacaaggta
    29821 ttaaactggc tgtggcatca aactcgaagc gtcaggatgt tttgttggcg ttggagacaa
    29881 cgcaaataaa agattatttt gaaataatcc tagcgcgtga agatgtttct agaggcaaac
    29941 cttatccaga tatctataat aaagcagtac aaaaactagg attacaaaaa aaacaactgc
    30001 ttgtagtaga agacagccaa aaaggcattg ctgccgccaa agcagccaat ctgacagttt
    30061 ttgccattac cgactaccga tatggcattg atcagagtca agctgatcac aagatagatc
    30121 atttaggaca actgtgtgta aaaatcggtt gttttggatc gtgaagtaac gcaactaact
    30181 gatcattgat gatacttcaa gacacttaag aatacgtcta ggactttcta cagaccaata
    30241 acaagaatac gtttatgtgt ttttactgtt tttttagcta cctatttaca atatgacact
    30301 gaaaagagta ctatgtttaa gttattatca aaaaaaccgt cgcataagat cacctagaaa
    30361 aggattatga tttttgtgat ttggatgcga ttgttagtcg ttgaccatct ttatgtggca
    30421 ggagatatgt ttaaccgtgg tcattatttg gatttgtttt tagattggct agtgagtaaa
    30481 tatagaagga tcataatact gctttgacaa aggcagtatc tgagtccatg agttgcacgg
    30541 ataatgtgac tcggttttgg ttagctataa taatatttgg aagtgttatg ctgcttgtct
    30601 tcgtaggtcc attgattata gtcagactta ctttagagct gagctgactg tctagacaag
    30661 acgtgatgga gatgtgattt ctgaccaagt agaggtaacc agaaaaggga cttttacgat
    30721 gtaattgaat ttacttgttg cattcttgtg catgttaaga gagaaaaccc agataaatca
    30781 ggaagtaaaa tggttatgtt tgattagaat tgtccttctt tttccttgaa gtcttaactt
    30841 attttgatta tgatagtagt atgactatta aggcagaaat taaggcactc gccaaagaaa
    30901 ttggtatctc taaaattggt tttacgacgg cagataactt tgattatttg gaaaagtcat
    30961 tgcgtgcttc tgtggaagag gggcgcaatt caggatttga acataaagtc atagaggatc
    31021 gtatctcccc tgaaaggctt ttagaatcgg ctaaaacgat tatttcaatt ggcgttgctt
    31081 atcctcacaa acttcctcag caacctcaaa aaacacctta taaacgtggc aaaattacac
    31141 ctagttcgtg ggggtttgat tatcattatg tggtcggcga aaagttagac agactatcaa
    31201 aaggcattga agagctgtgt cgagattttc ctttgcaaca aaaagcaatg gtcgatactg
    31261 gagctttagt tgatactgca gttgcccaaa gagcgggtat tggttttata ggaaaaaatg
    31321 gtctagttat ttctaaagaa tatggctctt atatgttttt gggagaatta attaccaatt
    31381 tagagatcga gccagacaag cctgtcgatt atgattgtgg tgactgtcgt cgttgtatag
    31441 atgcctgtcc aacttcttgt ttaattggtg atggctcaat gaatgccaag cgttgtctat
    31501 catttcaaac ccaagataag ggaatgatgg acatagagtt tcgaaaaaag attaaaacag
    31561 ttatctatgg ttgtgatatt tgccaaattt gctgtcctta taacaaaggt ataaataatc
    31621 caccagctac agagattgat cctgagttgg ctcaaccaga attaatacca tttttgagtt
    31681 tatcaaatgg acaatttaaa gaaaaatttg gtatgattgc tggaagttgg cgaggtaaaa
    31741 atattttaca gcgtaatgcc attattgcgc tagcaaatgc tcatgataaa accgcagtgg
    31801 tcaaattaat agagattatt gataaaaata ataaccctat tcatactgca acagctatat
    31861 gggcattggg agaaattgtc aaaaaaccaa acgatgagat actggcgttt atgagtcatt
    31921 taaccttaaa agatgaagat agccaaaaag agttggaatt aatccgtcat aaatggcaat
    31981 tttaagcctt ttatggtatt tttaagaacc ttatgataaa ataaaacaga ttaacaaaga
    32041 ttggagaaat ctcatggaag tggctgaaat tcgccaaaaa atagtagaaa ataaagagaa
    32101 gttgactagc ttcaggaggt ctctttgact tagatcgttt ggaagaggag attgctcttc
    32161 ttgaaaatca catgactgag ccagactttt ggaatgacaa tattgcagcg caaaagacat
    32221 ctcaagaact aaatgaatta aaaggtaagt atgatacttt ccataatatg caagaattat
    32281 ctgacgaaac agaactctta cttgagatgc ttgacgaaga tgattctttg aaagaagaat
    32341 tagaagaaaa tctaatgcag ctagataaga taatgggtgc ttatgaaatg acgcttctct
    32401 tatctgaacc ctatgaccac aataatgcta tcttagaaat ccatcctggt tcaggaggaa
    32461 ctgaagcgca agattgggga gatttactct tacgaatgta tacccgtttt ggcaatgcta
    32521 atggtttcaa agtagaagta ttggattatc aagcagggga tgaagctggt attaaatcgg
    32581 ttaccctctc ttttgaaggt cccaatgctt atggtttgtt gaaatcagaa atgggagttc
    32641 atcgcctcgt tcgtatatca ccttttgatt cggctaaacg tcgtcatacc tcatttgctt
    32701 cagttgaagt gatgccagag ttagatgata cgattgaagt agaagtacgt gatgatgaca
    32761 ttaaaatgga tactttccgt tcaggaggag ctggtggtca gaatgtcaat aaagtatcaa
    32821 caggtgttcg tttgacgcat attccaactg gaattgtggt atcgtctacc gttgaccgta
    32881 ctcaatatgg aaaccgcgat cgcgctatga agatgttgca ggctaagcta tatcagttag
    32941 aacaagaaaa gaaagcacaa gaagtagatg ctctcaaggg tgataaaaaa gaaatcactt
    33001 ggggaagtca gattcgttct tatgttttca ccccttatac gatggtgaag gatcatcgta
    33061 ctaactttga gcttgctcag gtagataagg ttatggatgg agaaatcaat ggctttattg
    33121 atgcctacct taagtggcgc attgaagact aaaaataata aaggagaaac atggcactaa
    33181 tagaaatgtc tggtgttact aaaaaatacc gccgctcaac gaccgccctc agagacgtca
    33241 atgtttctgt taaccaaggt gagtttgttt atctcgtagg cccatcaggt gctggtaaat
    33301 caacttttat caaactcctt taccgtgaag aacaactgac aactggtaag ctatatgtgg
    33361 gtgaattcaa tttaactaaa ctaaaagcac gtgatgttcc gattcttcgt cgtcacatcg
    33421 gagtagtttt tcaagattac aaactgctac caagaaaaac agtctttgaa aatgtagctt
    33481 acgccatgga agttattggt gaaaagcgcc gccacattaa aaaacgcgtg ccagaggtac
    33541 tcgatttagt tggcttgaaa cataagatgc gttcttttcc aagtcagcta tcaggagggg
    33601 agcagcaacg tgtggccatt gcgcgtgcga ttgttaacaa ccctaaatta ttgattgcag
    33661 atgaaccgac agggaattta gatcctgaga tttcatggga aattatgcag ttacttgaac
    33721 gtattaatgt tcagggaaca actatcttaa tggcgacaca caacagtcat attgtgaata
    33781 cattccgtca tcgtgttgtt gctattgaag atggtcgcat agtacgtgat gaagaaaaag
    33841 gagattatgg ttacgatgat tagatacttt ttccgtcata tttgggaatc cattaaaaat
    33901 cttaaacgca acttttggat gacatttgct tcagttagta tggtggctgt tacattgact
    33961 ttagttggtg tttttgcggc gacattgttg aatatacagc gtgttgcttc gggtgttgaa
    34021 aataatgtcc atatcaatac ctacttacaa gttgattcaa ctgatgctgc aaaagttatt
    34081 caaaatactg ccggtgaacc tgttaataac gataactacc acagtgttta cgacaaaatt
    34141 gctcagataa aaggtgtcaa aaagattaca ttttctagca aggatgaaca attgaaaaaa
    34201 ttgcaagaaa ccttaggtga tgtttggaat atgtatgacc aagacactaa tccgctccaa
    34261 gatatctatc ttattgagac ccagacgcct aaacaagtta aggcgatcac taaaaagatc
    34321 agaacgattg aaggcgtaga agccgcagat tatggaggga ttaactccga taaactattt
    34381 aagttttcaa cattaattca aacttggggg ctgatcggaa cagcaatgct attatttgta
    34441 gctgtctttc tcatttcaaa cactattcgt atgactatta tgtctcgtaa acgcgacatt
    34501 gaaattatgc ggttagttgg tgctaaaaat tcttatattc gagggccttt cttttttgaa
    34561 ggggcttggg ttggcttgtt aggagctgtt ttaccatctc ttctgattta ttatggatat
    34621 gatcttgttt ataagcattt tgcacaagaa ttacaacgta ataatctctc gatgtatccg
    34681 cttgatcctt atgtatatta cctgattggt gctttgtttg tcataggtat tatgattggt
    34741 tcgcttggtt cagtgctttc tatgagacgt tatttaaaat tctaaaaaag cgctgctgac
    34801 gaaacgtagc gaccctcaaa agttggacct cttatgttat tccaaggact tcgttttgta
    34861 ttgcacagga ctggtccttt tcattttgtt ttgattcatt tgtttatttg taataggcaa
    34921 tatagtttaa aatagcagtt tctaaggttt caagagaaga aaagcactcc aaaagttaga
    34981 tgtttgatgt atctaacttt tggggtgtca ttcaaaattt agtggctctt ttttagtaaa
    35041 atactatgat ttcagtttta ggataacctc aaacagtatc taaaatagct atgtcaatga
    35101 aaaaaggggt tagcattttt ttcgtgacaa atggtagtag aaggaccatg cccaggataa
    35161 actcggtaat gattaggtaa ggtgaatagt tcttggcgaa taccagtaat gaggtcctca
    35221 aagttactgg taggtaagtc agtacgaccg atagtttcac gaaatagagc atcgccagta
    35281 acgactaatt catctgagtg gaaaacaaat gagactcctc ccaaagaatg accaggtgtt
    35341 ggtaaaactg tgaattcaaa accatttagt tgatagggtt gctttaattt aaaaaagttc
    35401 tctgccggtc ttgctataac agtaatgata tcatcatgac ggcctaagcc agataaatta
    35461 tcatctgggg aagatagcca agcggcttct ttttctgaaa cataaacagg tggatgatca
    35521 aaagcatctc tgaccaaatc taaactaaaa atatgatcat agtgagtatg cgttaagagg
    35581 atagccacta aaggtttttc aaaggattta attttagcaa tgatttcttg gccgttactt
    35641 cctggatcaa tgaggataac agcttgatca ttaacgaggt aataagtatt ttcaccagca
    35701 atgtggttta ttgttttata gattttcata aggatagtgt accaaaaaat caagactttg
    35761 ttaaaaaaag agtagcggaa aataaatggc aattagtttc agaaaatatg agatctcaca
    35821 gcaatttctt gacaccgttt tcataaaggt gtatcataaa agtaatcaat aagccaagcc
    35881 taaagctgaa cttagtttaa gaacactgac tttttgagag caacctaatc ggttgactaa
    35941 aaaatatgag gtggaagata tgtcaaaagt tgctattgtt actggtgctg gtcaaggaat
    36001 tggatttgct attgcgaaac gtcttcatgc agacggcttt aaaataggca ttttagacta
    36061 taatgaagag actgctcagg ctgcggttga taaactatca cctgaggata ctgttgcagt
    36121 tgtagcagat gtttcaaaac gtgaccaagt ttttgatgct ttccaaaaag ttgtagatac
    36181 ttttggtgat ttgaatgttg ttgttaacaa tgcaggtgtt gccccaacaa cccctcttga
    36241 cacgattact gaagagcagt ttgaaaaggc ttttgcaatc aatgtcggag gaacgatttg
    36301 gggaagccaa gcagctcaaa aacattttag agaattaggt catggcggaa aaattataaa
    36361 tgcaacgtca caagcaggat gtgaagggaa tcctaatttg actgtttatg gtggtacaaa
    36421 atttgcagtt cgtggtatta cacagacttt ggcaaaggat ttggcatcag aaggaatcac
    36481 agtcaatgct tacgcaccag gtattgttaa aacacctatg atgtttgata ttgcccatga
    36541 agttggtaaa aatgcaggca aagatgatga atggggtatg gagcaatttg caaaagatat
    36601 cactttgaaa cgcttgtctg aaccagaaga tgttgctaat gcagtaggct tcctagcggg
    36661 agatgattca aattatatca ctggacaaac aatagttgtc gatggtggta tggtattcca
    36721 ttaaaaatga ttaatactga cactgtgagt gaaactcaca gtgtttttta tacttcttaa
    36781 aaaattatcc tatcttggtt attggagtga tggcaccttt gagatatagt catgttttgt
    36841 tttatagata tagcgtgcta taatagactt actatgaccc agaaaaaact tagaaaatat
    36901 gcagtagtgg acttggaagc tactggagca ggtcccaatg cttctattat tcaggttggg
    36961 attgttatta tccaaggaaa taaaattatt gatagttatg aaacagatgt taatcctcat
    37021 gaatctttgg atgagcatat tgttcatttg acaggtatta cggataagca gttggctaaa
    37081 gctccggatt ttggtcaagt ggcgcatcac atttatcaat tgattgaaga ttgtattttt
    37141 gttgcccaca atgtaaagtt cgatgcaaat ttattggcag aagctctttt tttggaagga
    37201 tatgagttaa caataccacg tgttgatact gttgaattag cccaactatt tttccctcgc
    37261 tttgaaaagt ataaccttgg tcacctatct aggcagctca atattgattt ggcagaagct
    37321 catactgcta ttgcagatgc aagagctaca gctatactgt ttttaagact tttacaaaaa
    37381 attgagagcc ttccaattga gtgcttagaa agcttattag tctattcaga tagcctgcta
    37441 tttgaaacag caatggtcat tcaagaagga ttagccaaag ccaaacctta tgatcccaac
    37501 aaatacatta aaatcagaca gattctctta ccaaagggct caaaagctct caagccttat
    37561 caaatatcaa aaagcttccc tattaatatg gcactattag gtctggaaga aaggcctaaa
    37621 cagactcaat ttgctcagtt aattgacgaa gattaccatc agggagtagc tagttttatt
    37681 gaagctcaga caggtattgg aaaaacctac ggctacctgc tacccctttt ggcaaaagaa
    37741 gatcaaaacc aaatcattgt tagtgtgcca accaaacttc ttcaagacca attaatggct
    37801 ggtgaagtag ctgctattca agaacaattt cacatcgcct gccatagttt aaaagggcct
    37861 gccaattacc taaagctaga tagctttgca gatagtttgg accaaaatga ccagaaccgt
    37921 ttggtgaatc gttataaaat gcaattattg gtttggctct tagaaactaa gacaggagac
    37981 cttgacgaaa tcaaacaaaa gcagcgcttt gccgcttatt ttgagcagct taagcacgat
    38041 ggagacatta agcagtcttc tgaattttac gattatgatt tttggagagt tagttatgaa
    38101 aaagccaaaa cagcccgtct cctgattacc aaccatgctt attttttaca ccgtattcaa
    38161 gatgacaagg actttactag aaacaaggtc ttggtctttg atgaggccca aaagctgatg
    38221 ctccaattag atcaattatc acgtcatcag cttaacctga cggttttttt acaaacaata
    38281 caggccaagt taagcaaccc tttacctctt ttagaaaaac gcttattgga aagcttgtcg
    38341 tttgaactgg gacaagtgtc aagtgattac tatcaaaata aagagcacca gcttgctcat
    38401 gattggtctc gtatagctgg ctacgccaaa gaattaacag gagcagatta tcaagagtta
    38461 caagcctttt ttgctacctc agatggtgac tactggctta gtagcgaaaa gcaagaggag
    38521 aagcgagtga cttatctaaa cagtgctagt aaggcattta ttcatttcca acagctctta
    38581 ccagaaacgg tgaaaactta ttttgtttct gccactttaa ccatcagctc agaagtaacc
    38641 ttggcagacc tattaggttt tgaagagtat ctctatcatg tcattgaaaa ggataaaaag
    38701 caagaccagt tggtgcttgt tgatcaagag gcgcctatcg tgacagaagt cagtgaccaa
    38761 atctatgtgg aagcaattgc aaaacgaatc gaaagcttga agcaagaggg ttatcctatt
    38821 ttagtgcttt ttaattctaa aaaacacttg ctgttggttt cagactatct agaccaatgg
    38881 caggtgcctc acttagcgca ggaaaagaat ggaacagctt ataatattaa aaaacggttt
    38941 gatcaaggag agcaaactat tttgcttggt ttaggttctt tttgggaagg agttgatttt
    39001 attcaagcgg atcgtatgat tacccttatc gcacgattgc cctttgataa cccagaggat
    39061 ttctttgtta agaaaatgag tcattatcta ttggaaaaag gtaaaaatcc tttcagagat
    39121 tacttcttgc caatgactat tttgcgatta aaacaggcta ttggtcgcac gatgcgtcga
    39181 aaagatcaaa agtcagtggt tattattttg gatcgtcgtc tccttacaaa gtcctatgga
    39241 caagttattt tggaaggtct cggtcaggaa tttttgattt ctcaacaaaa ttttcacgat
    39301 tgtctagtag aaacggactg ttttttgata taatagaaag gatctattat atcagaaaaa
    39361 tttttaatgg cgttaagcac aatcaactaa catttaaatt agtttgaact tttattatgc
    39421 tcttttagac aactgttcaa atgttttcga attgtttttt tgataaggac aataaatgaa
    39481 caaatttttg gttttagtaa acccaattac tgttcttttc attagtgtca gttgctatca
    39541 aaacagcttg tcatagttcg tgcgatcagt agcgagtatt ggtaaaactg aaaccgtaga
    39601 ttatgaaaaa atgatctgtt gatcattaat taatctgaag ataagaagtc gataaaaagg
    39661 agatacgatg cccaaactat caaagcgagt tttagaaatg aaagagagtg ttactctggc
    39721 tgcaggtgct agagctaagg ctttgaaagc gcagggccat gatgtactga atttaacttt
    39781 aggagaacca gattttttta cacctaagca cattcaagac aaggcgattg aatctattca
    39841 aaatggtacg gctagctttt atacaaatgc ttctgggctt ccagaattaa aagctgcgat
    39901 tgccacatat ctgaaaaatc agtatgggta tcacttgtct cctgatcaga ttgtggcagg
    39961 aacaggtgcc aagttcatcc tttatgcctt ttttatggct gttttaaatc ctggtgacca
    40021 agttctcatt ccaacaccat attgggtatc ttattctgac caagtaaaaa tggcagaagg
    40081 tcagccgatc ttcgttcaag gactagaaga aaatcaattc aaggtaactg ttgaccagct
    40141 ggaaagagca aggacaagta agactaaagt tgtccttatc aattctcctt ccaatccaac
    40201 aggcatgatt tatggtgctg aggaattaag ggccattgga gaatgggcgg tgcacaacga
    40261 tattcttatt ctggcagatg atatttacgg gtccttagtc tataacggca atcagtttgt
    40321 tcctatttca acactctcag aagcgattcg tcgtcaaacc attacggtca atggcgtggc
    40381 taaaagttat gccatgacag gctggcgggt tggttttgca gcaggtgaac cagagatcat
    40441 ttcggcaatg agtaagatca tcgggcagac tacctctaac ctgacaaccg tttcacaata
    40501 tgctgctatc gaggctttct gtggtagtca gtcatcactt gaagagatgc gattagcatt
    40561 tgaagagcgc cttaatatca cttatccatt gttgtgtcag gtccctgggt ttgaggttgt
    40621 taaaccacaa ggagcttttt acttctttcc aaacgttaag aaagccatgg aaatgacagg
    40681 tttttccgat gtgacaagct ttgccaatgc tattttagag gaagtcggat tagctgttgt
    40741 gagtggagca ggctttggcg caccggaaaa tgttcgtctg agttatgcta cagatatcga
    40801 aacccttaaa gaagctgttc gtagactgca tgtctttatg gaaagtaatg aaattaatta
    40861 aataaaaata gagagaaact tatgtcaaaa aaacttattt caattgttga tgttaaagat
    40921 tatgttggtc aagaagttac tattggtgct tgggttacta acaaatcagg taaaggtaag
    40981 attgcgtttg tacagttgcg cgatggttct gcttttttcc aaggtgtcgc ttttaaacca
    41041 aacttcattg aaaaatatgg tgaagaatca ggtcttgaaa aatttgacgt tattaaacgt
    41101 ctcaatcaag aaacatctgt ttatgtaaca gggattgtta aagaggatga gcgttctaaa
    41161 tttggctatg agcttgatat tactgacctt gaaatcattg gagagtcaca tgaatatcct
    41221 ataactccta aagagcatgg gacagacttc ttgatggaca accgtcacct ttggttgcgt
    41281 tctcgtaaac aaatggctgt tatgcaaatt cgtaacgcta ttatttatgc aacttatgaa
    41341 ttctttgacc aaaatggttt tatcaaattt gatagcccaa ttttatcaga aaatgcagca
    41401 gaagattcaa cagagttatt cgaaacagac tattttggga aaccagcatt tcttagccaa
    41461 tctggtcaat tataccttga agctggagca atggctctag gccgagtttt cgactttggt
    41521 ccagtctttc gtgctgaaaa atctaaaaca cgtcgccatt taacagaatt ttggatgatg
    41581 gatgctgagt attctttctt gtcacatgag gaatcacttg accttcaaga agcttatgtt
    41641 aaagcattga tccaaggcgt tctagatcgt gcgcctcaag cacttgatat tttagaacgt
    41701 gatgttgagg ctctcaaacg ttatatcaca gagccattca agcgtgtttc ttatgatgat
    41761 gctattacac ttcttcaaga gcatgaagca gatgaagata cagattatga acatttagag
    41821 catggtgatg actttggttc cccacatgaa acttggattt ctaattactt tggagtaccg
    41881 acatttgtag tgaattaccc agcaagcttt aaagcatttt atatgaaacc agttcctgga
    41941 aatccagaac gcgttctttg tgctgatctt ttagctccag aaggttatgg agagatcatt
    42001 ggcggctcaa tgcgtgaaga caactacgat gctcttgtgg ctaaaatgga tgaactaggt
    42061 atggataaat cagaatatga cttttacctt gaccttcgta aatatggctc tgttcctcat
    42121 ggcggttttg gtataggtat tgaacgtatg gtaacttttg ttgctggtac caaacatatt
    42181 cgcgaagcta ttccattccc acgtatgtta catcggatta gaccctaata atacaaaaaa
    42241 gttcatgtca tctatgaact ttttgtattg gtaagaagtg agtgtgttat aaaaacatca
    42301 atatgtccta aaggagagat aagaaatagt agagtttgaa cctacatcct ttgtgaagat
    42361 aaattttgga agtttagtag atcagttagc agataagaca atatagcttt gctcaaggga
    42421 tattttatag acaagtattt ggactacgat tatcaaatag acactctcat ttgatactaa
    42481 aaaggtctcg tctattcata taataggaga gctacgggag ttattattaa aattagctga
    42541 tttttacagt tcaaaatgtg ttaccacttt ttcacttctt agctttctta tgtatgtggt
    42601 ataatgaagg tggacggttc gatagagaaa gggaaattgt aaatgtcaga caaacacatt
    42661 aatttagtta ttgtgacagg aatgagcggc gctggaaaaa cagttgccat tcagtctttt
    42721 gaggatctag gctactttac cattgataat atgcccccag ccttggttcc aaaattttta
    42781 gaattaattg aacaaaccaa tgaaaatcgt agggtggctt tggttgtcga tatgagaagt
    42841 cgtttgtttt tcaaggaaat taattctacc ttagatagta ttgaaagcaa tcctagcatt
    42901 gattttcgga ttcttttttt ggatgcaacg gatggagaat tggtgtcacg ctataaagaa
    42961 accagacgga gccacccttt ggctgcggac ggtcgtgtgc ttgatggtat tcgattggaa
    43021 agagaactcc tatctccttt gaaaagcatg agccaacatg tggtggatac aacaaaattg
    43081 acccctagac aattgcgtaa aaccatttca gaccagtttt ctgaagggtc taatcaaccc
    43141 tctttccgta ttgaagtgat gagctttggg ttcaaatatg gtcttccttt ggatgcggat
    43201 ttggtttttg atgtgcgttt tctacccaat ccttattatc aggtagagct tcgtgaaaaa
    43261 acaggactag atgaggacgt ttttaattat gtgatgtctc acccagaatc agaggtgttt
    43321 tacaagcatt tgttaaacct tattgtccct atcttaccgg cttaccaaaa agaagggaag
    43381 tctgtcttga cggtggctat tggctgcaca ggaggccaac accgcagcgt tgcctttgcc
    43441 cattgcttgg cagaaagtct ggcaacagat tggtcggtta atgaaagcca tcgtgatcaa
    43501 aatcgtcgta aggaaacggt gaatcgttca tgaaaaatcc taaaatgact gttataggtg
    43561 ggggtactgg catctccatt atcctaaaaa gccttcgtaa tgaagcagtt gatattactg
    43621 ctgtggtcac tgttgcagat gatggtggtt cctcaggaga acttcgtaat gccatgcaat
    43681 tagctccgcc aggagactta cggaatgtct tgttagcgat gagtgacatg ccaaaatttt
    43741 atgagcgggt tttccaatac cgttttaacg agagtgatgg agccttggct ggtcaccctc
    43801 taggcaatct cattattgct ggtatctctg aaatgcaagg ctcaacctac aatgccattc
    43861 aaattttaac caaatttttc catattacag gtaaaattta cccctctagc gagcaggcct
    43921 tgacccttca tgctgttttt aaagatgggc atgaagtagc aggagaaagt tctattgcca
    43981 aatacccagg catgattgac catgtctatg tgaccaacac ctacaacgac caaaagcctc
    44041 aggccagtcg taaggtggtt gaagctattc tagaaagtga catgattgtc ttgggaccag
    44101 gctcactgtt tacctctatt ttacctaatt tggtcattcc agaaattaaa gaagctttgc
    44161 gccagactaa agcagaagtg gtgtatattt gtaatatcat gacccagtat ggtgaaacag
    44221 aacaattttc agatgcagac catgtggctg ttttaaatca gcacttagga agagatttga
    44281 ttgatacggt tttggtaaat gtggctaagg tgccgcaggc ttacatgaat tctaacaaat
    44341 ttgatgaata cctcgttcag gttgatcacg actttgctgg cctttgcagg gctgctaagc
    44401 gtgtgatttc gtcttatttt ttacggttgg agaatggcgg agcttttcac gatggtaact
    44461 tagtggtgga agaattaatg aatttagtga ggatagtcaa gcaatgagtt ttacaaccaa
    44521 ggtcaaagag gaattaattc acttgtccac aggtgacaac aatgaattgg ccgccattat
    44581 caaattatca ggcagcttgg gcttagctca ccaaagcctt catttatcca ttaccactga
    44641 aaatgctaaa attgcccgct acatttattc tcttattgaa gacgcctatg ttatcgtgcc
    44701 agaaatcagg tatcatcaaa agacgaacct gcgcaaaaac cgtgtttaca ccgtctatgt
    44761 tgagcaaggt gtggaaacca ttttagccga tttgaaatta gcggactcct tttttggtct
    44821 ggaaacaggc atcgaacctc aggttttatc agacgataat gctggccgtt cttatcttaa
    44881 aggggctttt ctagcggcag gtagcatcag agatcctgag tcaggcaagt atcagttaga
    44941 aatctattca gtttatcttg atcatgctca agatttagct caactcatgc aaaaatttat
    45001 gctagatgcc aaaaccatcg agcataaaag cggtgcagtt acttatgtgc aaaaggcaga
    45061 agatatcatg gactttttaa ttataattgg tgccatgtcc tgcaaagaag actttgaagc
    45121 catcaaatta cttcgagaag ctcgaaatga tattaaccgt gccaataatg cagaaacagc
    45181 taatattgcc aaaactatca gtgctagcat gaaaaccatt aataatatta ttaaaatcat
    45241 ggacacaatt ggtttagaaa gtttaccgat tgaattgcaa caggtagccc agcttcgggt
    45301 taagcatcca gactattcca ttcaacaagt tgctgatgct cttgagttcc caatcaccaa
    45361 gagcggggtc aaccatcgct tgcgtaaaat caataaaatt gcagatgact tatagaacat
    45421 ttttatcgta tgagcctttg ttcatacgat ttttttgtct aaaagtcgtt ttccattgtt
    45481 tttttgaagt gtctgatgtt ctgtgatatg ataaaaggga taataacgtt tgtaaaggag
    45541 actgataatg gcatgtacaa ctatactcgt tgtagtgcct aaataatgct tttaaaactt
    45601 aaaaataata tcgatgttat ccatagtaac ctcgactcta tctatcaatt gcttaactat
    45661 cctagattga acatcatacg aagattttct aatgtcaatg ttatccatca gcattccaag
    45721 agagtcttcg tttttcttgt ctaaataatt caatttaatt gctttgttat aatcatcttt
    45781 taaatgattg agttcttcga tgtctttttt tag
//
LOCUS       DAUGHY010000009        43665 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.009, whole genome shotgun sequence.
ACCESSION   DAUGHY010000009 DAUGHY010000000
VERSION     DAUGHY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43665
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.009"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            109..474
                     /locus_tag="VH752_000475"
     CDS             109..474
                     /locus_tag="VH752_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ8288849.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
     gene            511..1530
                     /locus_tag="VH752_000476"
     CDS             511..1530
                     /locus_tag="VH752_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEQ8288850.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGAACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            1984..2304
                     /locus_tag="VH752_000477"
     CDS             1984..2304
                     /locus_tag="VH752_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288851.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKSLADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            2294..4294
                     /locus_tag="VH752_000478"
     CDS             2294..4294
                     /locus_tag="VH752_000478"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEQ8288852.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPPIGKLTA
                     LRQKTPSLSFKQFEESHRQQAAQTALKMMSQKIERLEQLQNKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLSARVGTIPSTANDHFYHQLKQHKGLFIEEVYHTEFEYGLVLFWQ
                     AQDTIHLQKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQSQKILAQL
                     QVEIDYVLSQYQRQQTKKQLLGTRHLIALEGWIEADSVNQLKGLMTKTLGDMFYLDSY
                     DVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTFFGMMVAD
                     LGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVAIWGLIYGSFFGFDLPVALL
                     STKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFILLGLLLF
                     ILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGISSYLSDL
                     VSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLHAINIFLSMLSGYVH
                     GARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            4296..4775
                     /locus_tag="VH752_000479"
     CDS             4296..4775
                     /locus_tag="VH752_000479"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEQ8288853.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLISLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            4844..5428
                     /locus_tag="VH752_000480"
     CDS             4844..5428
                     /locus_tag="VH752_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEQ8288854.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKALEQQFQVAQQQLKNQERQALLALKQDSIKGLFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            5444..6442
                     /locus_tag="VH752_000481"
     CDS             5444..6442
                     /locus_tag="VH752_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEQ8288855.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLSHLLLPIGDKPLVALEHLIRTMTSDEFPKEVVTEIQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLVAALANDLPKERVIERMRPIA"
     gene            6439..6759
                     /locus_tag="VH752_000482"
     CDS             6439..6759
                     /locus_tag="VH752_000482"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEQ8288856.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDFGIIYITEDIAAAIPEALTHYDNQVLPAVIPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            6960..8735
                     /locus_tag="VH752_000483"
     CDS             6960..8735
                     /locus_tag="VH752_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEQ8288857.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHAGNFTVTDTVYEIKQEDGSIYQGSLMQTWPVRQSRPVAQKLIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     ESNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNILTFNEEASKALSLGAYFKEIMEGTAQVRDRIARSKFIPEE
                     NLEQIKGLTQKVTKEIHHVLAKGGI"
     gene            8736..10151
                     /locus_tag="VH752_000484"
     CDS             8736..10151
                     /locus_tag="VH752_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEQ8288858.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTKVRFTGHPLELAVSEDMVGRIFDGMGQPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGFYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            10196..10822
                     /locus_tag="VH752_000485"
     CDS             10196..10822
                     /locus_tag="VH752_000485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEQ8288859.1"
                     /translation="MARLNVKPTRMELSNLKNRLKTATRGHKLLKDKRDELMRRFADL
                     IRENNELRQTIEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNTAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYSIIPQLEETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(10942..12204)
                     /locus_tag="VH752_000486"
     CDS             complement(10942..12204)
                     /locus_tag="VH752_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEQ8288860.1"
                     /translation="MADFNFDIDQIADNAVVKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITAKAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLQEFYFDSQNIEQKMDSM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSATPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIINANNAALQMLAETSKEAIPA
                     LEQSAQNPTLSMKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKTTHA"
     gene            complement(12217..13095)
                     /locus_tag="VH752_000487"
     CDS             complement(12217..13095)
                     /locus_tag="VH752_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288861.1"
                     /translation="MIRKYDRTSTKKKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGTGYAIFRVRSHQKRLAKAKIAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPKIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHEANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDEDLDETLRRLNESDLKDFDISLR
                     IMQGTTQRRTTHHQKD"
     gene            13533..14825
                     /locus_tag="VH752_000488"
     CDS             13533..14825
                     /locus_tag="VH752_000488"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEQ8288862.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHDEGVTTDNLRISDRA
                     HVILPYHIQLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERL
                     RINLAEKNRLFEKMYDSTPLGFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWQEDITGVRSLDELPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWASI"
     gene            15152..16195
                     /locus_tag="VH752_000489"
     CDS             15152..16195
                     /locus_tag="VH752_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ8288863.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSSKQTTDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVIKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYAEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGKALVKVAVKTSEGQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIETAK
                     KAIIEGTITVPEN"
     gene            16368..16907
                     /gene="nusG"
                     /locus_tag="VH752_000490"
     CDS             16368..16907
                     /gene="nusG"
                     /locus_tag="VH752_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEQ8288864.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            17278..18633
                     /gene="nga"
                     /locus_tag="VH752_000491"
     CDS             17278..18633
                     /gene="nga"
                     /locus_tag="VH752_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HEQ8288865.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENK
                     KSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFVSPM
                     RIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDLFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAVQMSAVASRAEYYRMYVSRKYHYKKEFVEKLKQVYKESGASHVTS
                     KKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     KYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRMQDR
                     K"
     gene            18638..19123
                     /locus_tag="VH752_000492"
     CDS             18638..19123
                     /locus_tag="VH752_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HEQ8288866.1"
                     /translation="MYKVPKSLEHYQKMFQKEVTVNDLKKYLIGSDKEYRITRRDSYM
                     GDISDPEVILEYGVYPAFIKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSILANITKALKDAHIREAMVHYKLGEFAHYQDTMLDMVERTIET
                     F"
     gene            19147..20862
                     /gene="slo"
                     /locus_tag="VH752_000493"
     CDS             19147..20862
                     /gene="slo"
                     /locus_tag="VH752_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HEQ8288867.1"
                     /translation="MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETT
                     TTNEQPKPESSELTTEKAGQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDRTYPAALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVN
                     DPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKELQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRS
                     EYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            21117..21524
                     /locus_tag="VH752_000494"
     CDS             21117..21524
                     /locus_tag="VH752_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288868.1"
                     /translation="MKQQSYQPLRFVYLLVALFAAMLLIARPVMADGENNDPANAAYY
                     KGKSAGKEASWTDLTPTNPTIPEPPSDIRETTNKQHYKDGYTDGYRDGYNEGWKSQHP
                     VLTPVKVIWDLISHWLQQFFPNNQATTTVQSMS"
     gene            complement(21710..21946)
                     /locus_tag="VH752_000495"
     CDS             complement(21710..21946)
                     /locus_tag="VH752_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288869.1"
                     /translation="MKKKLSLFMIATAAVVSLSLATPENQTVSANDTQVTCVGCPDCG
                     PKWSCCPLTCMRDLLRGWGKSAAYYCTWGYYYSE"
     gene            complement(22356..22523)
                     /locus_tag="VH752_000496"
     CDS             complement(22356..22523)
                     /locus_tag="VH752_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288870.1"
                     /translation="MKKKLSLFMIATAAVAGLSLATLGSHQTVSANDASYSNGFYGHT
                     GPDSWLLYTVW"
     gene            23479..24618
                     /locus_tag="VH752_000497"
     CDS             23479..24618
                     /locus_tag="VH752_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEQ8288871.1"
                     /translation="MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELAQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMSFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            24829..27330
                     /locus_tag="VH752_000498"
     CDS             24829..27330
                     /locus_tag="VH752_000498"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEQ8288872.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTSDQ
                     AEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGKEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNENVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK"
     gene            27637..29070
                     /locus_tag="VH752_000499"
     CDS             27637..29070
                     /locus_tag="VH752_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ8288873.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAISSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGILQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            29141..29419
                     /locus_tag="VH752_000500"
     CDS             29141..29419
                     /locus_tag="VH752_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ8288874.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            29542..30027
                     /locus_tag="VH752_000501"
     CDS             29542..30027
                     /locus_tag="VH752_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ8288875.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVQLAVQPLIDSKAVTSAYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNRNAFALITLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKDSPYLEGMDLN
                     A"
     gene            30118..30780
                     /locus_tag="VH752_000502"
     CDS             30118..30780
                     /locus_tag="VH752_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEQ8288876.1"
                     /translation="MTHIPNLQVALDHSDLQGAVKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     MKEERGDRGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KTLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIKRI
                     WG"
     gene            30785..31648
                     /locus_tag="VH752_000503"
     CDS             30785..31648
                     /locus_tag="VH752_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEQ8288877.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDPAIEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVMLSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETKAAIKEAQDF
                     LYPLIEKAGLA"
     gene            31650..32354
                     /locus_tag="VH752_000504"
     CDS             31650..32354
                     /locus_tag="VH752_000504"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEQ8288878.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVARMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            32679..34325
                     /locus_tag="VH752_000505"
     CDS             32679..34325
                     /locus_tag="VH752_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEQ8288879.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INQALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQINDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            34578..35669
                     /gene="ulaG"
                     /locus_tag="VH752_000506"
     CDS             34578..35669
                     /gene="ulaG"
                     /locus_tag="VH752_000506"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEQ8288880.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLNHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSFEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            36157..37353
                     /locus_tag="VH752_000507"
     CDS             36157..37353
                     /locus_tag="VH752_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ8288881.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILENTEFGLELRGVPKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            37369..39096
                     /locus_tag="VH752_000508"
     CDS             37369..39096
                     /locus_tag="VH752_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEQ8288882.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGMFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGSGFVSGLALVILAIVLDRMTQLF
                     NSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMTMASGMADKGETVNIAYVQWDSE
                     VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKS
                     KLDDLGPNLKGTKLGLAVPKYMTDVNSIEDLSKQADQKITGIEPGAGIMAAAKKTLKE
                     YHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAKYDLKYLKDPKETFG
                     STENINTIARKGLKKDLPNVYKIIDKFHWTQKDMEAVMLDINKGMSPEAAAKKWVEAN
                     KSKVSSWTK"
     gene            39227..41869
                     /gene="polA"
                     /locus_tag="VH752_000509"
     CDS             39227..41869
                     /gene="polA"
                     /locus_tag="VH752_000509"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEQ8288883.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVRPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPDEFREQFSYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKTSKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAVISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     REAIIGFAWGHGEQIYASTDLSLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRATLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGA
                     DIHTSTAMRVFGIDKAADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQSKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            42056..42511
                     /locus_tag="VH752_000510"
     CDS             42056..42511
                     /locus_tag="VH752_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEQ8288884.1"
                     /translation="MIYSFQNPSEDILKAYLESAKTIAVVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEVQTILRSAGKEAIVMNRCLKIDYLQLVAKPECITNL"
     gene            42563..43030
                     /gene="perR"
                     /locus_tag="VH752_000511"
     CDS             42563..43030
                     /gene="perR"
                     /locus_tag="VH752_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEQ8288885.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICPDCQAKDQSDF"
     gene            43187..43486
                     /locus_tag="VH752_000512"
     CDS             43187..43486
                     /locus_tag="VH752_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288886.1"
                     /translation="MFLTWENLFLTNQSRRRLKLLVVERSYNNVGNFLKEYFGGFFEI
                     INFDDLRLTMVDMVSLSSEYDVIVTDMILEQTMDSEILFFNQMAPSVVANRLTDM"
BASE COUNT        13226 a         8072 c         9415 g        12952 t
ORIGIN      
        1 ttgccaatca ccttaataat aattttttat caaaaagaga atttattatt gactgtgaat
       61 gcggtttcct gtaaaattta actatattat caatgaggag gttcgtatat gaagagctac
      121 ccagaaccaa tgggccctta ttcaacttat acgattgaag gacattttct atacaccgca
      181 ggccaattac cccttaatcc agtgacaggt caattgtcag atggttttga ggcacagtgt
      241 cgccaggttt ttgttaattt acaatctatt ttggcagagc aaaagttgga cttgaatcat
      301 atctataagc taaatgtgta tttaacagat gtgaccaatg tggaaatatt aaatcatgtc
      361 atgactgatt tgtttgaaga gccttaccct gttcggacag cggttcaagt gtcggcgctt
      421 cctttgcaag ctttgattga agtcgaagct gttgccagag tgaagcagtt atagtgtctt
      481 atgtaatgag atagattggt ggaatagatt atggatatta ttattggaac aagtcttttg
      541 attcttgttt tagctatttt tagcttgttt aactataagg ctcctcatgg tgccaaggca
      601 atgggagctt tagcttcggc agcttgtgcc tcttttttag ttgaagcctt tcaagattct
      661 ttttttggta aggttttagg ctttcagttt ctaagtgaag taggcggagc aaatggctca
      721 ttatcagggg tagctgctgc tatcttggtt gccattgcaa ttggtgtgtc tccaggttat
      781 gccgttttga ttggtttgtc agtttcagga acaggtatta tcccaggttt tgtggctggg
      841 tatgtggtat cttttttaat caagtggatg gaaaaaaata ttcctggtgg gcttgattta
      901 atctcgatta ttattgtcgg tgcaccatta actcgtttct tggcgcagtt aattacgcca
      961 gtgatcaata gtactttatt gactattgga gacatcctga cctcaagtgc taatagcaac
     1021 cctattatca tgggaatgat actaggcggt acgattgttg ttgtagcaac agctccgcta
     1081 tcatcaatgg ctttgacagc aatgcttggt ctaacaggta ttccgatggc tataggtgcc
     1141 ttatctgtct ttggctcttc ttttatgaat ggtgttcttt tttaccgatt aaaattgggt
     1201 gaaagaaaag acaatattgc ttttgctatt gaaccgttga ctcaggcaga tgtgacgtca
     1261 gccaatccca ttccgattta tgtgactaat tttgttggtg gtgctgcatg tggtgttttg
     1321 atcgccctaa tgaaattggt taatgatacg ccagggacag ctactccgat tgccggtttt
     1381 gcggtaatgt ttgcctataa tcctgtcgct aaagtgctga taacagccct tggatgtatt
     1441 attatcagct taattgtggg ttatattgga ggttctgtct ttaaaaatta ccgtttagta
     1501 acaaaacaag aattacaagc gagaaactaa taaacatctt tttgacagga cttttaacac
     1561 gatagcagtt atactgttat cacattggct tggctattgg ctggtaaagg ttccttaccg
     1621 tcaactagat caaggttact aaatgcctaa agaagtataa gataacacac gaaagacagc
     1681 aatacaaagc tttagagttc tgtgtcatct tactgctttg acaggttttt agctaacctt
     1741 tttctgaact aagccattgt ttttatggat agaattgaaa gcgcttaact tacagatgct
     1801 gttcgtttaa tcaagcaatt atcctatctc aaaaaagctg ttagtagata ggttagagat
     1861 ttagctctag cttatagtcg ctggttgatt aacccccaaa agtagccttt cttgtagctt
     1921 tgtttttaag tacctagtga gtcaaagaat tagcaataga taaagataag gagttgatat
     1981 gtgatgaata ccctattgtc actaatgaat agtatcgaac aagaggctag aacaatttat
     2041 gacagttacg atgctaagaa aaaatctcta gctgataaaa aagaagaaga gttaaagcag
     2101 ctgttccttg agtacgaaca agaaactcag ctactattag aagctaaacg tgaggtgttt
     2161 tttgaagcta atcgtcaaga acaaaaagct tcaaaaaaga ggttagcgac gcaacagcta
     2221 gcccttcatg ccattttaga agagcataag ctaaccgttg ttagggagtt agtggataag
     2281 gtggtggaga catatggcca ttagtcaaat gaaaaagctg gcaatggttt ttgaaaaaga
     2341 ttatcttgat cttgttttaa aaacacttca acaatctcaa ttagttgaag ttcgtgacat
     2401 gaagcaacta aaacattggc aagatgcttt taacaaaggc aacgtcaagt tgccacaaat
     2461 cgtccaatac gatctgacgc atcaaaaacc tctactagat gatgaagctt tacaatacct
     2521 tttacaaagc caacaagagt tagaaaacgg ccttgctagc ttatcagcgt ttttaccacc
     2581 aataggaaag ttaacagcct tgagacaaaa aacgccatct ctttcgttta aacagttcga
     2641 agaaagccat cgacaacaag cagctcaaac ggctttgaaa atgatgtcac aaaagataga
     2701 gcgactagag cagctgcaaa acaaaataga ccagctaaca gaatactgtc aggagttaga
     2761 aaagtggcgt tccttgactg ttttgcctca ggacttagcg caatttcatt ttttgtcggc
     2821 aagagttgga acgattccta gtacagcaaa tgatcatttt tatcatcagc taaagcagca
     2881 caaaggtctt tttattgagg aagtatacca tacagaattt gagtacggcc ttgtcttatt
     2941 ttggcaagca caagatacga ttcatttaca gaaatatcag tttaaaccct tattatataa
     3001 agaacagcta ctgccatcag aacaacttcg tataaacaaa gagttactaa cgaattggct
     3061 agctgaaaaa gatagtctac taaaagagct taggcagtct caaaagatac tggcacagtt
     3121 acaggttgaa atagactatg tccttagtca gtatcaacgg cagcaaacta aaaagcaatt
     3181 actcggtaca agacatctga tagccttaga agggtggata gaagctgatt cggttaacca
     3241 attaaaaggg ctaatgacta agacactagg tgatatgttt tatctggata gttacgatgt
     3301 cacgccagac gattgggaag atgttcccat taagctacgt aatcaccgct acattgcacc
     3361 gtttgaattg gttacagaaa tgtacgcttt gccaaaatat caagaaaaag atccgacacc
     3421 ttttttagca cctttatacc ttactttttt tggcatgatg gtagctgatt tgggctatgg
     3481 tttgttgcta tatgctgtga cactagctgc attggtcttt tttaatctcc aaaaaaccag
     3541 taaacggtta gtgaccttct ttaatatttt agctatttca gtagccatat gggggcttat
     3601 ttacggttct ttctttggtt ttgacttacc tgtggcatta ttatcaacta aaacggatgt
     3661 gattactatt ttggttgttt ctttgctatt tgggtttgtt acacttatat ttgggctttt
     3721 gttaggagca tggcaacaag taaggatgaa agcatatgct acggcctata cgtcaagtct
     3781 agcttggact tttattttat taggcctgct gttatttatt cttggtaaga atgtatctgg
     3841 ccttgcttac ttatcggtta ttggtaagtg gcttgcattg ggcaatgcct ttgggatttt
     3901 ggttgtttct ttactaaaaa gcaaaagcct tttaggtcta ggtagtggat tatataattt
     3961 atatggcatt agctcttact taagtgattt ggttagcttt actcgtctaa tggcattagg
     4021 gctttctgga gctagtattg gagcggcttt taacatgatt gttgggattt ttccaccagt
     4081 cacgcgcttt acagtaggta tctttatttt tattcttttg catgcgatca atattttctt
     4141 atcaatgctt tcaggatatg tgcatggtgc tcgtcttatt ttcgtagagt tttttggaaa
     4201 attttatgaa ggtggaggaa aagctttcaa tcctttaaag ttagctgata attatgtcaa
     4261 tgtcaatgaa gaaacagatt tggaggacaa ttaacatgga acatttagca cattatttta
     4321 cagcacatgg tggcgttttc ttcgcagcct taggaattgt tttagccgta gctcttagtg
     4381 gcatgggctc agcttatggt gtcggtaaag gtggtcaagc ggcagctgcg ctcttaaaag
     4441 aagaacccga aaaattcacg tctgcgctta tcttgcaact gttacccggt agtcaaggaa
     4501 tctacggctt tgctattggt attttaattt ggatgaaact gacaccagaa ctttcagtta
     4561 accaaggatt agcttacttt ttgatatctc ttccaattgc tattgttggt tatttttcgg
     4621 ccaagcacca aggcaatgtt tctgtagctg gtatgcaaat cttggctaag cgccctaaag
     4681 actttatgaa gggtgtcatt ttagcagcta tggttgaaac ctatgctatt cttgcctttg
     4741 tcgtctcctt tattctctta aataaaattg gataaggtaa cggtccttat ctagatttta
     4801 aaaaacggtg acaccactaa cactatcaaa agggggaaat taggtgaatg atataacaca
     4861 gttaagacaa aacgttcttg aaaaagcaca tcaagaaggt caacagtgcc taaagatagc
     4921 gacagatagt ctcgatactg actttaaaga gcgtcaacag caaggactac acgatttaaa
     4981 agcaaagcgt caaaaagagt taaaagctct tgaacagcaa tttcaggttg cgcagcagca
     5041 actcaaaaac caagaaagac aagctctact agcattaaaa caagacagta taaaggggct
     5101 atttgaagct tctttggaga agatgacgaa cttttctaag gaagaagaat tggctttttt
     5161 gaagcaagtc ttgtctaaat atccagaaca gccattgcaa gttacttttg gcgaaaaaac
     5221 agggcaaaag ttcagttctt atgattgtgc tgagctgaga ttagcgtttc ctcaattaag
     5281 ctataatcaa gagctaattc ctcaggaagc aggattttta gtcagtttag accaagtcga
     5341 tgacaattat ttataccgtt accttttaga atcagttctt aaagaagaaa gctcccgcat
     5401 cattgatatg cttttttcag agatatagga ggtttgacta gcgatgccaa catttctaga
     5461 attaaacact actatcagtg tcaaagaaaa agagttattg actaaagagc aatttgataa
     5521 gctattgcag gctcccaata caacaacctt agctcgactg ttgcaccagt cagtctatca
     5581 cctaactgtt gacgatctca acgatttgga tcggctagaa tctatcttaa tggctgagct
     5641 aaccaagacc tatcgctggg cttttgcaga aacccctcaa cctgacatag tacaattatt
     5701 taccttgcgt tacacctatc ataatgtcaa agtgttgtta aaagctaaag ctagtcaagc
     5761 agatctgagt cacttattac taccaattgg tgacaagcct ttggtggctt tagaacattt
     5821 gattaggacg atgacttctg atgagtttcc aaaagaggtg gtaacagaaa tccagtccat
     5881 ttgggccgaa tatcaagatt atcaagacat acgcgtctta gaaattggta ctgacttagc
     5941 ttattttaag gcgttgaaac aaatagcaca aagattagag gaccctgttt tccaacaagc
     6001 tgttcttatt gtgattgatt tgtataatct gattaccgtc agacgggcca aatcgcaaaa
     6061 taaaccaata agtttcatga tgcagttatt atctgatgaa gcaagtagac caagtaaaac
     6121 atttatcaca cttgaagatg ataaggatct gatgacgtgg tttgaaaatg taacacctga
     6181 tagctatatg acagccctaa aaccttattc agagaaactt cgccaaggca ctcttcaaac
     6241 gactgagtta gaataccttg ttgatgaatg cctttaccat ttatttgcga aggccaaata
     6301 tcaagtcgat ggaccttatg tgcttgctcg ttttttgcta gctaaatcgt ttgaagtaaa
     6361 aaacctacgg cttttagtgg ctgctttagc taatgattta cccaaagaac gtgtcataga
     6421 aaggatgaga ccaatagcat gaccaaaaca ttgtataaag tcggggtaat tggtaacaga
     6481 gatgttattc taccttttca gatgattggg tttcaaactt ttccagtaat aaaaccacaa
     6541 gacgctatta accagttgcg tcaactagca atggaagact ttggtattat ttatatcaca
     6601 gaagatattg ctgcagctat tccagaagcc ctcactcact atgacaatca agttctacca
     6661 gcagtcatac cattaccaac tcatcaagga gcgcaaggga taggcctgtc acgcatacaa
     6721 gccatggttg aaaaggctgt cggtcaaaat attttataac actaagacgt taggcaaaga
     6781 ggacttatac tctgtttgag gcttatgagt agctcactga tgaaatgcaa ggaaaggaaa
     6841 attggtgaag ttagcagctg aacgcatagg atagtttgct gactattcaa aatagtcaca
     6901 ttaaaccttg acagtataga ttagttagta tgatttggaa aaaataaagg aggaaaaagg
     6961 tgaaccaagg aaaaataata accgtttcgg gacctcttgt tgtggcttct gggatgcaag
     7021 aagctaatat tcaagatatt tgtcgtgtgg gacatcttgg cttagtcgga gaaattattg
     7081 aaatgcgccg cgatcaagcg tctattcagg tttatgagga aacatcaggg atcggtccag
     7141 gagaaccagt agtgactact ggttgtcctt tgtcggtcga gttaggccca ggcctgattt
     7201 cagaaatgtt tgacggtatt cagcgaccgc ttgatcgttt tcaaaaagca acggacagcg
     7261 actttttaat ccgtggtgtg gctatcccaa gtcttgatcg aaaggctaag tgggcattta
     7321 ttcccaagct aagtgttggt caagaagtag ttgcaggtga tattttagga actgtgcaag
     7381 aaacagctgt cattgagcat cgtatcatgg ttccttataa agtttcaggg accttggtgg
     7441 ctattcatgc agggaacttc acagtaacag atacagttta tgaaattaag caggaagacg
     7501 gttccattta ccaaggtagc ctcatgcaga cttggccagt tcgtcaaagt cgccctgttg
     7561 ctcaaaagct tatcccagtc gagcctttgg ttacaggtca acgggttatt gacacctttt
     7621 tccctgttac aaaaggtggt gccgctgccg ttcctggacc atttggggca ggaaaaacag
     7681 ttgtgcagca tcaaatagct aaatttgcca acgttgatat tgttatttat gtcggttgtg
     7741 gggaacgcgg caacgagatg accgacgttt tgaatgagtt tccagagtta attgacccaa
     7801 atacaggcca gtccattatg gagcgcacgg tgttaattgc aaacacctct aatatgccag
     7861 tagcagcgcg tgaagcgtcg atttacacag gcattaccat tgccgaatat ttccgtgata
     7921 tgggctattc tgtggctatc atggcagact cgacatcacg ttgggcagaa gctctgcgcg
     7981 agatgtcagg gcgcctacaa gaaatgcctg gtgatgaagg ctatccggct tacttaggga
     8041 gtcgtattgc cgaatattat gaacgggctg gtcgtgttcg gaccttggga agtcaagaac
     8101 gtgagggaac cattacagcc atcggcgcgg tttctcctcc tggaggagat atttcagagc
     8161 ctgtcactca aaacaccctt cggattgtca aagttttctg ggggctcgac gcgcctcttg
     8221 cgcaacggcg tcacttccca gcgattaact ggctgacgtc ttattcattg tatcaagatg
     8281 atgtaggaag ctatattgac cgtaaacagg aatctaattg gtccaacaag gtaactcgtg
     8341 ccatggctat tttgcagcgt gaagccagtc tagaagaaat tgtacgcttg gtggggcttg
     8401 attcactgtc tgaacaagat cgtttgacca tggctgttgc ccggcaaatt cgggaggatt
     8461 atctccagca aaatgccttt gattcggtgg atacctttac ttcctttccg aaacaagagg
     8521 ccatgctaac caatattttg acctttaatg aggaagccag caaagccctt tctttgggag
     8581 cttattttaa ggagattatg gaaggcactg ctcaggtacg cgatcgcatc gcacgcagca
     8641 aatttatccc agaagaaaac ttagagcaga ttaaagggct tactcagaag gttaccaaag
     8701 agattcacca cgttttagca aagggaggaa tttagatgag cgttctcaaa gaatatcgga
     8761 cggttagcga agtggttggc cctttgatga ttgttgatca agttgcagga gtgcactaca
     8821 acgaattggt agatattact ttgcataatg gagaaaggcg taaaggtcaa gtcttagaag
     8881 tccaaggtga caaggctatg gtacagctat ttgaaggatc aactggcatc aacctagcca
     8941 aaaccaaggt tcgatttaca gggcatcctt tggaattagc cgtatcagaa gatatggtag
     9001 ggcgcatctt tgatggaatg gggcagccca tagacggcgg tccagaactt attcccgaaa
     9061 aataccttga tatcgatggt caagcgatca atcctgtcgc tcgtgattac ccagacgaat
     9121 ttatccagac aggtatctca gctattgatc acctcaatac cctcgttcgt ggtcaaaagt
     9181 taccagtttt ttcgggctca gggttacctc ataatgaatt ggccgcacag attgctcgtc
     9241 aggcaactgt tctaaactct gatgataact ttgcggttgt gtttgcagct atgggtatta
     9301 cttttgaaga ggcagaattc tttatgaatg acttgcgtga aacgggtgcc attgatcgat
     9361 ctgttttatt tatcaatttg gctaatgacc ctgctattga acgcattgca acaccacgta
     9421 tagctctgac aacagccgaa tacttggctt atgaaaaagg tatgcatgtt ttagtcatca
     9481 tgacagatat gacaaattat tgtgaagcct tacgagaagt atcagccgct cgcagagaag
     9541 ttccaggcag acgaggttat cctggctatc tctatactaa tctctccacc ttatatgaac
     9601 gggctggtcg tctgatcggg aaaaaagggt cagtgactca aattcctatt ttaaccatgc
     9661 cagaagatga cattacccac cctatccccg atttgacagg ttatattacc gaaggtcaga
     9721 ttattttgtc gcaagaacta tataaaaatg gttttagacc gcctattaat gtcttgccat
     9781 cgctatctcg cctaaaagat aaaggctcag gtgaaggtaa gactcgacaa gaccatgctg
     9841 ccacaatgaa tcagctcttt gcagcctatg cccaaggaaa acaagctaag gaattggctg
     9901 ttgtgctcgg tgaatcagcc ctttctgaaa cggataaact atatgtggcc tttaccaacc
     9961 ggtttgaaga agaatacatt aaccaaggat tttacaccaa ccgtagcatt gaagaaagtc
    10021 ttgacttggg ttgggaattg ttgtcgattt tgccacgtac cgaattaaaa cgcattaaag
    10081 atgatatgtt agatcgttat ttgcctaaag cagataccac gatgacaaag gtttttgttg
    10141 caaatgattg agttgaccat gcaatttcaa gcccttgacg aaaggagtta tatcaatggc
    10201 acgattaaat gttaaaccaa cacgcatgga acttagtaac ttgaaaaatc gcttgaagac
    10261 agctacaaga ggtcataaat tattaaagga caagcgggat gagttgatgc gtcgcttcgc
    10321 tgatttaatt cgtgaaaaca atgagcttcg tcaaactatc gaaaaagaac ttgctgccaa
    10381 tatgaaggag tttgtcttgg caaaagcatc cgaaaatagt ctaatggtag aagaactctt
    10441 tgctgttcca gtccatgaag tgaccttatg gattgatatt gaaaatatca tgagtgtcaa
    10501 tgtgcctaaa ttccatgtcc agtctaatac ggcaagagag caagaacaag gtgagtttgc
    10561 ttatagttac ctcagctcaa atagtgagat ggacaatact atccaaaaga caaaagaact
    10621 attagaaaag ctcttacggt tagctgaagt tgaaaaaact tgtcaactaa tggctgatga
    10681 cattgaaaag actagacgtc gcgtcaatgg cttagagtat tctattatcc cgcaactgga
    10741 agagacgatt cactacattg agttgaaatt agaagaggcc gagcgtgcta gccttgttcg
    10801 tattatgaaa ataacatcat aaaagataag tgttatatca aacaaggcga ttattcgtta
    10861 agtagtaaaa aagcaagaca tcaaagggat taacctaatt gatggcttgc tttttaagta
    10921 gcgtgacagg tgatgtttag cttatgcgtg agttgtttta tcaattgttt tttgagtttc
    10981 cttgccttca ctaagaagtg cttgcacaat attttggtca cgtagtttga cagaatcgtt
    11041 aatggtttcg gcagacctga taatagcact ttctagttgt gctcgttctt tgcgtccatg
    11101 atcaatcgcc gcaatgatcc cattattttg tgctactaaa ctttctgcca gacttgtcac
    11161 agatttcata gacaaagtag ggttttgtgc tgattgttcc aatgctggga tggcttcttt
    11221 gctggtttcc gccaacatct gaagggcagc attattagca ttaataatgg cgtcagcagt
    11281 cattccagat ttaaccgatt gttgcatcat gcctaactga gcaatagata gtttcatcgt
    11341 tggaatggta ttgcggcgta acatgcctag tttttgacgc atgtcagaag agaccttgac
    11401 tagattacgc atttgcggag tcgtcgccca agcaacatag aggcgactca agtattcggt
    11461 gtgttgttgt tcgagggtgt tgatgacttc ggtagttctt gccaaaagat cagcttttat
    11521 ttgatagtct ggtgtagcac tgtctttggt ttttaggtct ttttgtaaag ccgcagcgcg
    11581 ctgactagct tctttttgac tagcttcgat aaaagcaatg acgcctacca aatgttcaat
    11641 ggacttagta ttatcttcaa ttaacaattc tgctgagaca atattgcgag ctaaggtatc
    11701 ttcttgcttg acaaccgctg cagccatact atccattttt tgctcaatgt tttgagaatc
    11761 aaaataaaac tcttgcaggg tatcacggct ttgtttaaag agtttttgaa gaaagtttgg
    11821 ctttttatca aggtccacag gtgtggcatc cttgtattta gcgataaaac cattgagttc
    11881 acgattggtt gattttaaaa gatcatcaac ctgaggaatc tgtaattttt tttgctctgc
    11941 caagatatga ttaaccgttg cattgacccc ttcaacagca gactgaccaa aatcaagtag
    12001 cgcattttgg tctgccaaaa aggtatctac caatgccggg gctttggcag taatagctgt
    12061 ctgctggtca gcacttagtt tctcaaaaaa agagatttgg ccatttgtgt cagttggtaa
    12121 gtctgagata atatcggtgg ttttatcggt tttgacaaca gcattgtcag caatttggtc
    12181 aatgtcaaaa ttaaaatctg ccataaaggg actcctttag tctttttggt gatgcgttgt
    12241 tcgtctttga gtagtacctt gcatgattcg aaggctaata tcaaaatctt taagatcact
    12301 ttcattgagt cgacgaaggg tttcgtcaag atcttcatca aattgttgga tagcttgttt
    12361 ggcttgttct aaacgagcag cagcgttata gtaattttta ggctcttctt tgatttttaa
    12421 gtagcctgtt aagatatcct caaaccttct catgttagct tcatgcagcg caagcaattc
    12481 ttcttgattg tcggctcctt ggattttagc aaggatagct tgatgatctg tctgaatatt
    12541 atcataatac ggtttgagct caggtgcttt gttaataaga ttaggagatg gggttttcaa
    12601 aggctgaggg ttaagagttg tggcaatctg cagtgtttcg agttgaaggg taatatcact
    12661 ttcaacgtca ttggcttttt tggtgatacg acgataaatt ttgggatcta actggtcttt
    12721 taacgtaata gcttttgctt tgatgtcact aagttgtggt aataattgct gagcaagtac
    12781 gttatattgg aagtcatcat ggtctgccag ataagtgtct aacagccgaa ctttacgatc
    12841 agctagctga atttttgctt ttagatcttc taattgtttg gcaatcttgg cctttgctag
    12901 ccgcttttga tgagatctaa cacgaaaaat agcataacct gtaccaccaa tggctatggc
    12961 aggcaaaatg tctagaagtg actcagtaaa agaagagcca cctataaagc ttgaaatcat
    13021 aaaaaaagca atgattagcc aaatccagtt tagtgatttc ttttttgtgc tcgtgcggtc
    13081 atactttcta atcatgtgat atttcctcga tatgttttta ctggctatct catagaggta
    13141 acttactatt attttacctt aaaaccgcta tttctgaaag aaaagagcta atgttttaag
    13201 agaatcgttt aaccctgcta aagcttagga gaaacgaggt taaaagcttt tctcttataa
    13261 tgttcgtttt tttaggattg tttatcatgg ttaaaaaaag agtttctctt tttccttaat
    13321 aatgctaaga aatctagcag aaaaaatcat tttttgctat aataagttgt aaggtatatg
    13381 ctagcaggtg agattcctgc ctgttttctt taaatatcaa gagctacatt gctgatctta
    13441 cagatctgac cttctttttg gtttttaagc agtggttagc ttgcctatga tttttattga
    13501 gaataagtgt ttcagaaagt taggacaatg atatgacatc tgtagttgtt gttggtaccc
    13561 agtggggtga cgaaggtaaa ggtaaaatca ctgatttctt gtcagcggat gcagaagtaa
    13621 ttgctcgcta tcaaggtggt gataatgctg gtcatacgat cgtgattgat ggtaagaagt
    13681 ttaaacttca cttgatccca tcaggaattt tcttcccgca aaaaatttcc gttattggta
    13741 atggcgttgt ggtcaacccc aaatctttgg tcaaagagtt ggcgtacctg catgatgaag
    13801 gagtgacgac agataacctt cgcatttcag accgagctca tgttatttta ccttatcata
    13861 tccagctgga tcaactgcaa gaagatgcaa aaggtgacaa taaaatcgga acaactatta
    13921 aagggattgg tccagcttat atggataaag ctgctcgagt tggtatccgt atcgcagatt
    13981 tactggataa ggatatcttt gcagagcgcc tacgcattaa tttagctgag aaaaaccgtt
    14041 tatttgaaaa aatgtatgac agtacacctc ttggttttga tgctatcttt gaggagtact
    14101 atgcttacgg tcaagaaatc aagcaatatg tgacagatac ttctgtgatt ttaaatgatg
    14161 cacttgatgc tggtaaacgt gttctttttg aaggtgctca aggggttatg cttgatattg
    14221 accaaggaac ttacccattt gtaacgtctt caaacccagt tgctggtggt gtaaccattg
    14281 gttctggtgt tggcccaagt aaaatcaaca aagtagttgg tgtctgtaaa gcctacacaa
    14341 gccgtgtcgg tgatgggcca ttccctacag aactctttga tgaagtgggt gagcgcattc
    14401 gtgaagtggg tcatgagtac gggacaacga ccggccgtcc acgtcgtgtc ggttggtttg
    14461 actcagttgt tatgcgccat agtcgtcgtg tgtcagggat tactaacctc tctctgaatt
    14521 caattgatgt tctttcaggg cttgatacgg ttaagatttg tgtggcttat gaccttgatg
    14581 ggaaacgtat tgactattac ccagcaagcc ttgaacaact caaacgttgc aaaccaatct
    14641 atgaagaatt accaggctgg caagaggaca tcacaggtgt tcgtagcctt gatgagcttc
    14701 ctgaaaatgc ccgcaactac gttcgtcgtg ttggagaatt ggttggcgtt cgcatttcaa
    14761 ccttctcagt tggtccaggg cgtgaacaga ccaatattct tgagtcagtc tgggcatcta
    14821 tctaaggtgt ctagtatgac aaagagttgg atagcgtttc cagctctttt taccgcttgt
    14881 tggattgtat agttagtcat gctattacta gtagcttgag ttttgtcgtt gtcttatctc
    14941 cttaagaggt gatttttgag gagataacgg gttttaccag ttgatctcac tttctcgttc
    15001 ggtcattttg gtgaaaaaga gatttttcag caaaaatagt aggataaaac tgattttttt
    15061 gatatactaa acttttcgtt ctagagcata agtagaacgt taacaatgac acatcacttt
    15121 aggattaaac atttaggagg ttcattttca catgaacaaa aaagtaatgt cacttggtct
    15181 tgtttcgact gccctattca cattaggagg ctgcaccaat aactcctcta aacaaacaac
    15241 tgacaattca ttaaaaatcg ctatgatcac taatcagacg ggtattgatg acaagtcatt
    15301 taaccagtca gcctgggaag gcttacaagc ttggggaaaa gaaaataaac ttgaaaaagg
    15361 aaaaggctat gattatttcc aatcagccaa tgaatcagag tttaccacaa accttgagtc
    15421 agcagtaacc aatggttata atcttgtttt tgggattgga tttccattac atgacgctgt
    15481 agaaaaagta gccgcaaaca atcctgacaa ccattttgca attgtggatg atgtgattaa
    15541 aggtcaaaaa aatgttgcaa gtatcacctt ttcagaccat gaagcggcat acctagccgg
    15601 tgttgcagca gctaaaacga caaaaaccaa gcaagttggt tttgtaggtg gtatggaagg
    15661 agatgttgtc aagcgctttg aaaaaggttt tgaagctggt gtgaaatcag tagatgatac
    15721 catcaaagta agagttgctt atgcaggctc ttttgcagat gctgccaaag gcaagacgat
    15781 tgcagctgct caatatgctg aaggcgcaga tgttatttat catgcagcag gaggcacagg
    15841 ggcgggtgtc tttagcgaag ctaagtctat caacgaaaaa cgtaaagaag aagataaggt
    15901 ttgggttatt ggtgttgacc gtgaccaaag tgaagatgga aaatacacca caaaagatgg
    15961 caagtcagct aattttgttt tgacctcaag tatcaaggaa gtcggaaaag ctttagtaaa
    16021 agtagccgta aaaacctcag aaggccaatt cccaggtggt caaataacca cttttggttt
    16081 aaaagaaggt ggtgttagcc ttacaacgga tgctctgaca caagacacta aaaaagctat
    16141 tgagactgct aaaaaagcga ttatcgaagg aaccatcaca gttcctgaaa actaaaactg
    16201 tacaataccg tccagagtcg tcacgaaccg actttggact ttttaataaa ggtgtcaagt
    16261 agctccacag atacgttgac accagccctg tcttttatta taatgaaggt gatagaaaac
    16321 agcgcaaaag ccaaaaaaag ggctttttta taatgaaagg aaacatcatg ctagattcat
    16381 ttgataaggg ctggttcgtc ctccaaactt actcaggcta cgaaaacaaa gtaaaagaaa
    16441 acctcttgca acgtgcccaa acctataaca tgttggacaa tattttgcgc gtcgaaattc
    16501 cgacacaaac ggttaacgtt gaaaaaaatg ggcaaaccaa agaaatcgaa gaaaatcgct
    16561 ttccagggta tgtcttggtt gaaatggtca tgacagatga agcttggttt gttgtgcgta
    16621 acacaccaaa cgttacaggt tttgtgggat cacacgggaa ccgatcaaaa ccaacaccac
    16681 ttcttgaaga agaaatccgt gctatcttgt tgtctatggg tcaaaccatt gatgtctttg
    16741 ataccaatat caaagaaggt gatgtggttc agatcataga cggtgccttt atgggacaag
    16801 aaggtcgtgt ggtagaaatc gaaaacaaca aagtgaagct gatgctcaac atgtttggct
    16861 ctgaaacagt agcagaagtt gaactttatc aaattgcaga actataggct ctcaaaggtc
    16921 tgatggtata agtattacca tcagaccttt tttagttggc agtgaagcgt gtttacaaac
    16981 caatggatga ctattgttaa catcattgta atcagaaata attaacagat ctattactga
    17041 taacggtgct actatcaaat atttaactag tacttttaat ctggtttaaa tagaacagat
    17101 gtgaaggttc tgtgataata agatggaaat atccccttga aacttgtaac agttggctac
    17161 ttgttttttt gtgttcattt ttttgataga aaagcctaaa acgatgttaa taaatcgcat
    17221 tgacaaaaac tagttgttaa tgtatagttt acttaaaaat aatataaggt ggtttacatg
    17281 agaaacaaaa aagtaacatt agctcatatt gtcgcaaaga caagtgttgc tattgctttg
    17341 gctggagcaa tgggtagcag tttattagct aatagcacaa cgtacgctgt tagtggcaaa
    17401 gaaaataaaa aaagcgatgt caaatatgaa acgaccaaag ttatggaagc taacgcaact
    17461 tcctctaaag aagacaatca tgtcatgcac acattagacg gctcaatgag tactgtctgg
    17521 gaggaaaatt cacctggtgg tggcgttggt gaggtacttt cctacaagtt tgtttccccg
    17581 atgcgtattg ggagaatttt aattgtgaat ggagatacat ctagcaagga gaattactac
    17641 aagaaaaata gaattgcaaa ggctgatgtt aaatactata acgggaataa attggtcctc
    17701 tttcaaaaaa ttgaattagg cgacacctac actaaaaaac cgcatcacat tgagattgat
    17761 aaaaaattag atgttgatcg tattgatatt gaggtaacag aggtccatca gggacaaaac
    17821 aaggatattt tagccttgtc agaggtcact tttggcaata tggaacgcga tctttttgaa
    17881 aaaaagttta aagaaattaa agataaatgg gtaacagata aacaagcaga tgagtttatt
    17941 gaaactgccg acaagtatgc tgataaagct gttcagatgt ctgctgttgc gtcacgtgct
    18001 gagtattacc ggatgtacgt tagccgcaaa taccactaca aaaaagagtt tgttgaaaaa
    18061 ctaaaacaag tctacaaaga aagcggagct tctcacgtta caagtaaaaa agatttgatg
    18121 ttagcttttg atgatgctaa aagaaagtca acgattggtc gacaagaaaa cggtcttttt
    18181 gtgacaagtt ttgctgaaga tatggcattg ctctttactg atcaaggtaa gttaaaatca
    18241 gctgaccaaa ttgaaaatat aaaaggtgtc gatagcggaa aatatagcga tggagtttat
    18301 caatatgagt acgattctga actaacaaaa aacattgata agctaggcta tatccgaaca
    18361 gctagcggag atacccctgg agcaaattcg ctcaacattc ctggttgcca aacgtggtca
    18421 ggaaaacaca ttgaaaattc agaaagtgag ttaattttcc catcgattag tgttaaagat
    18481 ctaaaatcta aagctgtctt agcagagatt gatgccaaag gctattttga aattattgat
    18541 cctaccatca ttgctccaaa tggtgaccat aaaaaagtaa ctggtcgctt caaaattaag
    18601 aaaatgcaag atagaatgca agataggaag taacaatatg tataaggtgc caaagagttt
    18661 agaacattac caaaaaatgt ttcaaaaaga agtaactgtt aatgatttaa aaaaatactt
    18721 gataggtagt gacaaagagt atcgtatcac tagaagagac tcctatatgg gagatatttc
    18781 tgatccagaa gttattttag agtatggggt ttaccctgct ttcataaaag gctataccca
    18841 attgaaagct aacatcgaag aagcattatt agaaatgtca aatagcggtc aagcattaga
    18901 catttaccaa gcggttcaaa ccctaaacgc tgaaaacatg ttattaaatt attacgaaag
    18961 cttgccattt tatttaaacc gtcaaagcat actagctaat ataaccaaag cgttaaaaga
    19021 tgcgcatatt agagaggcta tggtacatta caaattagga gaatttgctc actatcaaga
    19081 tactatgctt gatatggtcg aaagaacaat agaaacattt tagaatgata aaaaggtatg
    19141 aaggacatgt ctaacaaaaa aacatttaaa aaatacagtc gcgtcgctgg gctactaacg
    19201 gcagctctta tcattggtaa tcttgttact gctaatgctg aatcgaacaa acaaaacact
    19261 gctagtacag aaaccacaac gacaaatgag caaccaaagc cagaaagtag tgagctaact
    19321 accgaaaaag caggtcagaa aatggatgat atgcttaact ctaacgatat gattaagctt
    19381 gctcccaaag aaatgccact agaatctgca gaaaaagaag aaaaaaagtc agaagacaaa
    19441 aaaaagagcg aagaagatca cactgaagaa atcaatgaca agatttattc actaaattat
    19501 aatgagcttg aagtacttgc taaaaatggt gaaaccattg aaaattttgt tcctaaagaa
    19561 ggcgttaaga aagctgataa atttattgtc attgaaagaa agaaaaaaaa tatcaacact
    19621 acaccagtcg atatttccat cattgactct gtcactgata ggacctatcc agcagccctt
    19681 cagctggcta ataaaggttt taccgaaaac aaaccagacg cggtagtcac caagcgaaac
    19741 ccacaaaaaa tccatattga tttaccaggt atgggagaca aagcaacggt tgaggtcaat
    19801 gaccctacct atgccaatgt ttcaacagct attgataatc ttgttaacca atggcatgat
    19861 aattattctg gtggtaatac gcttcctgcc agaacacaat atactgaatc aatggtatat
    19921 tctaaatcac agattgaagc agctctaaat gttaatagta aaatcttaga tggtacttta
    19981 ggcattgatt tcaagtcgat ttcaaaaggt gaaaagaagg tgatgattgc agcatacaag
    20041 caaatttttt acaccgtatc agcaaacctt cctaataatc ctgcggatgt gtttgataaa
    20101 tcagtgacct ttaaagagtt gcaacgaaaa ggtgtcagca atgaagcccc gccactcttt
    20161 gtgagtaacg tagcttatgg tcgaactgtt tttgtcaaac tagaaacaag ttctaaaagt
    20221 aatgatgttg aagcggcctt tagtgcagct ctaaaaggaa cagatgttaa aactaatgga
    20281 aaatactctg atattttaga aaatagttca tttacagctg tcgttttagg aggagatgct
    20341 gcagagcaca ataaggtagt cacaaaagac tttgatgtta ttagaaacgt tatcaaagat
    20401 aatgctacct tcagtagaaa aaacccagct tatcctattt catacaccag tgttttcctt
    20461 aaaaataata aaattgcggg tgtcaataac agaagtgaat acgttgaaac aacatctacc
    20521 gagtacacga gtggaaaaat taacctgtct catcaaggtg cctatgttgc tcaatatgaa
    20581 atcctttggg atgaaatcaa ttatgatgac aaaggaaaag aagtgattac aaaacgacgt
    20641 tgggacaaca actggtatag taagacatca ccatttagca cagttatccc actaggagct
    20701 aattcacgaa atatccgtat catggctaga gagtgcaccg gcttagcttg ggaatggtgg
    20761 cgaaaagtga tcgacgaaag agatgtgaaa ctgtctaaag aaatcaatgt caacatctca
    20821 ggatcaacct tgagcccata tggttcgatt acttataagt aggactggtt caagaggttc
    20881 gtcaagcacc ttgatgctgc ttatctcttg aggccttgtg cctaatcctc tcacaaatac
    20941 agaagaaagc ttgtctatga gataccttca tagacaagct tttttgatat actaatcaaa
    21001 aaattattct tttctcacat cgtgaccttg aagctaagct gcttctaatc tataatacta
    21061 acaagctgct ttttaacaag caaagcataa cataaaaggg agagataact attatcatga
    21121 aacaacaatc ttaccagcct ctacgcttcg tctacctctt ggtggctcta tttgctgcta
    21181 tgttgcttat agcaagacct gttatggcag atggggagaa taatgaccca gctaatgcag
    21241 catattataa agggaaaagc gctggaaaag aagctagttg gactgatttg accccaacta
    21301 atccaaccat tccagaacca cctagtgata tcagagagac tactaataaa cagcattata
    21361 aagacgggta tacagatggg tacagagatg gttataatga aggctggaaa tctcagcatc
    21421 ccgtcttgac tccggtcaag gttatatggg atttgatctc tcattggcta cagcaattct
    21481 tccccaataa ccaggcaact acaacagtac aaagcatgtc ataataaaaa tgaattaaaa
    21541 acagccttta actgctaatg tgaaaggttg tttttttagg atatgggaat gaacaataaa
    21601 aaaagagttt gtgaaaaagc ctctcggcca cacaaaaaaa caatggcgtc aaagccattg
    21661 tttttttagc ataatcaaga gactactgtc tcagactctc aagtaatttt tactcagagt
    21721 agtaatatcc ccaagtgcaa taatatgctg ctgacttgcc ccaacctctt aacaaatcgc
    21781 gcatacaggt caatggacaa caagaccatt ttggaccaca atctggacat cctacacaag
    21841 taacttgcgt gtcatttgct gaaacagttt ggttttctgg tgttgcaagg ctaaggctta
    21901 cgacagcagc agttgcgatc ataaataaag ataatttctt tttcatttgt aactccttaa
    21961 attatagtat acctatatta tactcatatt atgttaatga ttcaatatca cattttaatc
    22021 tttttgttat gtgtttgacc gaaaaaggca aagaaagccg atactaaagc atttctttat
    22081 cttatataaa taacattaat tattaataat gttaatctgg ttataattat atttgaggtg
    22141 ttacagcttt tttggtggtg gttagctagc aactgttacg acttgtgttt tgggcttgtc
    22201 cttttatagg cggtatgcct tttcttttta atcttgaggg atcgttagta aaaacgtttt
    22261 agccaatcaa aaagcctaga actgcttacg cagctctagg cttttcataa ccgtagtctt
    22321 acgaagaaga tagcttctat gttacggtat taggtttacc atactgtgta aagtaaccag
    22381 ctgtcaggac cagtgtgtcc gtagaaaccg ttactatagc tagcatcatt tgctgaaaca
    22441 gtttggtggc ttcctagtgt tgcaaggcta aggcctgcaa cagcagcagt tgcgatcata
    22501 aataaagata atttcttttt catttataac tcctttaatt tgtattgtga ttatattata
    22561 ctaacttttt gttaataaat caagtgttat tttaatgttt ttattattat ttatctgaat
    22621 aagaagtgtg gcgacctagg attagaaaac cgccacgaga tggttattta aattccatgg
    22681 atatgattat agagatctta gatgaacaag ttttttgcag aattccacaa atttgttatt
    22741 aagctccata accagtttaa cagggaagtc ttggtgtttg aagatgaatt tgaagttata
    22801 ctgtctcctt ctgataaact atgccagcca gcgcatacag atccatcgtt agtacacgtc
    22861 attgagctgg tttggggttc ttttgcctgc tcagccgcat gcacggtctg atgatgaggg
    22921 gttgctaaag agggccctag gacagttacg gtcgcaaggg caactaaaaa taatttgttt
    22981 ttcactatat ttctctccta aaaataaata gtattttagc ggtatactat aagacgcatg
    23041 gtgaaaacaa gttctgtttc tcattaaatg aaataaattg tgacctttta atgaaatttt
    23101 gatggcagta ttgccatatt gagtaggtga cgatgcaagg taggactgtc agtgtgataa
    23161 tttgagcttt tgagacgatg gggccaaagg tagctatact ttaacttgct ggagaggtgt
    23221 tggcagcttg gtcaaagatg ataaaagggc ttgcaaactt agctaaaggt tgtataataa
    23281 acgtaatcga tctaaaaatg atattattgg aaaaattcat tatccattta aaaggtgccc
    23341 actacttttc tttatttttc ttataggtta gcagatcacg cttttttgaa gagctttttg
    23401 gtttgtgcaa ccattggggt gggaccattg tctaaaagag agttcattgg agttaacgct
    23461 attgcggagg tgcacgaaat gagagaaaat acgaccttat tacatggcta tacggttatt
    23521 gacgagttta caggagcagc ttcagttccc atctatcaga cttcgacgtt ccacaattca
    23581 gaactgtatt gtcctagtca aaaacatttg tacacacgct tttcaaaccc aacgactgaa
    23641 gctttagaag acgggttagc ctgtttagaa aaagcgactt atgcggtggc ttatgcatct
    23701 ggtatggctg ctatttcaac tgttttgatg ctcttaaaag ctggagatca tgtgattttc
    23761 ccattggagg tctatggagg gacttgtcaa tttgcgacag ctattttacc caattatcag
    23821 attgaaacaa gctttgtaga tatggctgat ttagcaacag taaaagcaag tatcaggcct
    23881 aatacgcgta tgatttatct agagacaccg tcaaatccgc tcttgaaaat ttgtgatatt
    23941 tcagagcttg cgcaactcgc caaagcttat ggtgtgctaa ctgtggctga taataccttt
    24001 atgaccagtt tgtatcaaga acccttagct atgggtgtgg atattgtagt agaaagtgtg
    24061 accaaattta tcaatggtca tagtgatgtg gtagctggtt tagctgctac caataatgag
    24121 gctatctaca accagctcaa gctttttcaa aaaaactttg gagcaattgt tggggtcgaa
    24181 gacgcttggc tgattcttcg tggcatgaaa actatgggga tccgtatgga acaagcggta
    24241 aaaaatgctc agcagctcgc taactattta gcgaagcacc ccaaagttct taaggtacat
    24301 taccctggct tagacagtca tcctaatcac gatacccacc tgcaacaagc taagaatggt
    24361 ggtgctgttt tatcctttga gttggcttct aaggaggagc tgatgtcctt tacccatcgt
    24421 attcagttgc cgattttagc cgttagtcta ggtggagtag agtccatttt gtcacatcca
    24481 gcaacgatgt ctcatgcttg tttatcgtca caagcgcggt tggaacaagg ggttgttgat
    24541 ggtttgctgc gcttgtcttg tggtgttgaa aatatcgaag acttattggc cgattttgag
    24601 caagcacttg ctttttaagt ctatttgtcc agattggctc tgtttacaga gcaagtctgg
    24661 cttttttatt agctttttgt gaggatggtt gatgctaaag cagggtttaa aaatagctaa
    24721 tcgtctgaag tctcaaaagc caaggtgcta gtcatgtctt gaccattcct aaaagcttca
    24781 tctcatgcta taataagaag actagatttt ttacatggga gatttcacat gacattttac
    24841 gatcacaccg ctattgaacc caaatggcaa gccttttggg cagataatca cacctttaaa
    24901 acaggaacag acgcatcaaa acctaagttt tatgccctag atatgttccc atatccatca
    24961 ggagctggtc tccacgttgg tcacccagaa ggctatacag caactgatat tcttagccgt
    25021 ttcaaacgtg cccaaggtca caatgtcctc cacccaatgg gttgggatgc ctttggactt
    25081 cctgctgagc agtacgctat ggatactggt aatgatccag ccgagtttac agctgaaaac
    25141 atcgctaact tcaaacgcca gattaatgct cttggcttct catacgactg ggaccgtgaa
    25201 gttaacacaa ctgaccctaa ctactacaag tggacgcagt ggatctttac caagctttat
    25261 gaaaaaggtc tagcctacga agctgaagtt cccgttaact gggttgaaga attgggaaca
    25321 gctatcgcca acgaagaggt tcttccagat gggacatcag aacgtggcgg ctatccagtt
    25381 gtcagaaaac ccatgcgcca atggatgctc aaaatcactg cctatgcaga gcgcttgcta
    25441 gaagacttgg aagaagttga ctggccagaa tcaatcaagg acatgcaacg caactggatt
    25501 ggtaaatcaa ctggcgctaa tgtaaccttc aaggttaagg atacagataa ggacttcact
    25561 gtctttacaa cacgtcctga cacgcttttt ggtgcaactt acgccgttct tgctcctgag
    25621 catgccttgg ttgatgctat tacgacatct gaccaggcag aagccgtggc tgactacaaa
    25681 cgtcaagcaa gtctcaaatc agaccttgct cgtacagacc ttgccaaaga aaaaactggt
    25741 gtctggacgg gtagctatgc gattaacccg gtcaacggca aagaaatgcc agtttggatt
    25801 gcggactatg tccttgctag ctacggtact ggtgctatca tggctgttcc agcccacgat
    25861 gaacgtgact gggaattcgc aaaacaattt aacctagaca tcatccctgt tcttgaaggt
    25921 ggtaatgttg aagaggctgc ctttacagaa gacggtcttc acatcaactc cgacttccta
    25981 gatggtcttg acaaagcgtc agctattgcc aaaatggtcg aatggcttga agcagaaggc
    26041 gtcggtaatg aaaatgtcac ttaccgtctc cgtgactggc tctttagccg tcaacgttac
    26101 tggggtgagc caatcccaat catccactgg gaagatggga catcaacagc ggttcctgag
    26161 agtgaattgc cattggttct tccagtaact aaggatattc gtccttcagg tactggtgag
    26221 agcccgcttg ctaacgtgac agactggttg gaagttaccc gtgaagatgg tgttaaaggt
    26281 cgtcgtgaga ctaacaccat gccacaatgg gcaggttcaa gctggtatta tcttcgttac
    26341 atcgatccac acaacaccga aaaactggct gatgaggaac tcctcaaaca atggttgcca
    26401 gttgatatct atgtcggtgg tgcagagcat gccgtgcttc accttctcta cgctcgcttc
    26461 tggcacaagg ttctttacga tctcggtgtt gtacctacca aagagccatt ccaaaaactc
    26521 tttaaccaag ggatgatttt ggggacaagc taccgcgata gccgtggagc gcttgtagcg
    26581 actgacaagg ttgaaaaacg tgatggcagc ttcttccatg tggaaactgg ggaagagctt
    26641 gaacaagcac cagctaagat gtctaaatca cttaagaacg tcgtcaaccc agatgatgtt
    26701 gttgagcagt acggtgctga cacccttcgt gtttacgaga tgttcatggg accacttgac
    26761 gcttccatcg cttggtctga agaaggtctt gaaggctcac gtaagttctt ggaccgtgtt
    26821 taccgtttga tcacgactaa agagatcact gaggaaaata gcggagccct tgacaaggtt
    26881 tacaacgaaa ccgttaaggc cgttactgag caagttgacc aaatgaaatt caacacagct
    26941 atcgctcaac tcatggtatt tgtcaatgca gccaacaagg aagacaaact ctttagcgac
    27001 tatgccaaag gctttgttca attgattgca ccatttgcac cgcacttagg tgaggaactc
    27061 tggcaagcct tgacagcctc aggtgagtca atctcttacg tgccatggcc aagttatgat
    27121 gaaagcaaat tggttgaaaa cgatgtggaa atcgttgtcc aaatcaaagg taaggtcaaa
    27181 gctaaacttg tagttgctaa agacctttca cgcgaagaat tacaagaagt tgcccttgct
    27241 aacgaaaaag tccaagccga aatcgcaggc aaagacatca tcaaagtcat cgcagtacca
    27301 aacaaactcg ttaatattgt tatcaaatag tttgaggtga cacagccctt cagtggttat
    27361 ttgcgtcacg tcacagttaa tgccaaagag ctgatctatt caaaaagcaa ctcatctaga
    27421 aaaaccacaa gaacgaaggt tcttgtggtt tttaatcgtt gtttagggtg attttctcct
    27481 tattattaag tgtagttaaa ttttaaaata agttaactta agtctaatat tgaacacttc
    27541 tttgtgtgat agttggtctt ttttaaaacg ctttcattac ctataatgaa catataaaca
    27601 ctttatcttt attaactttg aaaagaggta gttcttatgg aaatgctcct agctccccta
    27661 aactggttct cgcaaaatat tttgcaaaac ccagcgttct ttgtaggtct tttggtttta
    27721 atcgggtatt tactccttaa aaaacccatt tatgaggtct ttgctggttt tgtaaaggct
    27781 accgttggtt acttgatttt gaacgttgga gcaggcggtc ttgtaacgac ttttcgtccg
    27841 atcttggttg ctcttgccaa gaaatttgaa ttgaaagccg ccgttattga tccttatttt
    27901 ggtttggctg ctgctaatac taagttagaa gagatgggct tcatcagtgt tgctacgaca
    27961 gctctcttga ttggatttgg ggtcaacatt cttttggttg cacttcgcaa ggtcactaaa
    28021 gttcgtaccc ttttcatcac aggtcacatc atggtccaac aggcagcgac gatttcggtc
    28081 tttgtccttc ttttaatccc tcaattccaa aatgcttttg gggcatgggc agtcggtatc
    28141 atctgtgggc tttactgggc tatcagctca aacatgaccg ttgaagcaac tcaacgcctt
    28201 acaggtggcg gtgggtttgc gattggtcac caacaacaat ttgcgatttg gtttgtggat
    28261 aaggttgcac cgttctttgg taaaaaagaa gaaaaccttg ataacctaaa attaccaacc
    28321 ttcttaaaca ttttccatga tactgttgtt gcgtcagcaa ctttgatgtt ggtctttttc
    28381 ggagctatct tggctgtcct tggtccagac attatgtctg atgtggactt aatcggacct
    28441 ggagcattca acccagctaa gcaagctttc ttcatgtata ttttgcaaac ctctctcacc
    28501 ttctcagttt atctctttat cttgatgcag ggtgttcgca tgtttgtttc tgaattgacc
    28561 aatgcctttc aagggatctc aagcaaattg cttccagggt cattcccagc ggttgacgtt
    28621 gcagcttcct atggctttgg ttcttcaaat gcggtgcttt caggatttgc ctttggtttg
    28681 attggtcaat taatcaccat tgctcttctt gttatcttta aaaatccaat cttaattatc
    28741 acaggattcg ttcccgtatt ctttgataat gcggcaattg cggtttatgc agataaacgt
    28801 ggtggctgga aagcagcggt agctctatca tttatttcag gtattttgca agttgcactt
    28861 ggtgctgttg ctgtaggtct tttaggactt acaggtggtt atcacggtaa tatcgacttg
    28921 gttcttccat ggttaccatt tgggtacctc ttcaaattcc ttggtattgc agggtatgtg
    28981 cttgtttgca tcttcttact tgccattcca caattgcaat tcgcaaaagc taaggacaaa
    29041 gaagcttatt accgtgggga agctcaataa cactgcatct taagaaatag ctctgctatt
    29101 ttaacaaaac aataataaaa cattttcgga ggaaaaaaca atggttaaag tcttaacagc
    29161 atgtggcaat ggtatggggt catctatggt gatcaaaatg aaagttgaaa atgcgcttcg
    29221 tcaacttggc gtgacagata ttcagtcagc ttcttgctca gttggtgaag ctaaaggtct
    29281 agcttctggc tatgatattg tagtggcatc aaatcacttg attcatgagc ttgatggtcg
    29341 taccaaaggc cacttggttg gtcttgataa cctgatggat gataatgaaa ttaaaaccaa
    29401 actacaagaa gtgctataag ctaattctga atcaaaacga aggagttgtg acgaaggttt
    29461 tcagctcttt ctctttagta ggataaaact agtaaaaatg gtgtgtgttg caaagcaaga
    29521 cactagaaag agatagtttt tatgaattta aaacaagcct ttattgacaa caactccatc
    29581 cgattggggc taagtgctga tacttggcaa gaggctgttc aattagctgt tcaacctttg
    29641 attgatagca aagcagtgac gtcagcttac tacgacgcca tcattgcatc aacagaaaaa
    29701 tacggccctt attatgtgtt gatgccaggt atggctatgc cccatgcaga agcaggttta
    29761 ggcgttaatc gcaatgcatt tgctttgatt acactaacaa aaccagttac cttctcagat
    29821 ggtaaagagg tttctgttct tttgacgttg gccgctacag acccttctat tcacacaaca
    29881 gtggctatcc cacaaattgt ggccttgttt gaactagata acgccattga acgcttggtg
    29941 gcctgccaaa gtcctaaaga ggttctagaa atggtagaag aatctaaaga tagtccttac
    30001 ttagaaggta tggacttgaa tgcttaagct ttccttacac aggatactaa cgaatgatgg
    30061 aaaggataag aactcttaac cagtttgcac ggtggctaag agttttagga aaaagagatg
    30121 acacatattc caaatttaca agttgcttta gaccattctg acttacaggg ggctgttaaa
    30181 gcagccgttg ctgtgggtca cgaagtagac gttatcgaag ccggtacggt ctgcttgctt
    30241 caagtcggaa gcgagttggt tgaggtatta cgcagcctct tcccagagaa aattatcgta
    30301 gcagatacca aatgtgctga cgctggcggt acggtcgcta aaaacaatgc aaaacgtggt
    30361 gctgactgga tgacctgcat ttgctgtgcg acgattccaa ccatggaagc agccctcaag
    30421 gctatgaaag aagagcgcgg tgaccgtggg gaaattcaaa tcgaacttta tggtgattgg
    30481 acttatgagc aagctcaatt atggcttgac gctgggattt cccaagccat ttaccaccaa
    30541 tcacgtgatg cccttcttgc tggagaaact tggggtgaaa aagaccttaa caaggtgaaa
    30601 acattgattg acatgggctt ccgcgtgtca gtaactggtg ggcttgatgt agacactttg
    30661 aaactttttg aaggggttga tgtctttacc tttatcgcag ggcgaggcat tactgaagca
    30721 gaagatcctg ctgcagcggc gcgtgccttc aaagatgaaa tcaaacgtat ctgggggtaa
    30781 ctttatggcg cgtcctattg gtatttatga aaaagcaacg ccaaaacagt ttacctggcg
    30841 tgaacgcttg caatttgcaa aagacttagg ttttgatttt gtggaaatgt ctgttgatga
    30901 atcagatgct cgcttagcgc gcttagagtg gactaaggaa gaacggcttg atttagtgaa
    30961 agccatttat gaaacaggca ttcgcatccc gaccatttgt ttttctggtc atagacgtta
    31021 cccactaggc tcaaatgatc ctgctattga agccaagtcc cttaaactga tgaaacaatg
    31081 tattgaattg gcacaggact taggcgttcg taccattcaa ttagctggtt atgacgttta
    31141 ctatgaagaa aaatcgcctg aaacccgtgc tcgttttatc aaaaacttga gacaatcttg
    31201 tgactgggcc gaagaagccc aagtgatgct ttctattgag attatggatg acccttttat
    31261 caactctatt gaaaaatact tggcagttga aaaagagatt gactcgcctt atctctttgt
    31321 ttatcctgat gcaggcaatg tttctgcctg gcataatgat ttgtggagtg aattttacaa
    31381 tggtcacaag tccattgcag ccctccactt gaaagatact tatgctgtca cggaaacctc
    31441 aaaagggcag tttcgtgatg tgccatttgg tcaaggttgt gtggactggc aagagctgtt
    31501 tgcggtcctc aagaaaacca actacaatgg gccttttttg attgaaatgt ggtcagaaaa
    31561 ttgtgacacg gtggaggaaa caaaagctgc tatcaaagaa gctcaggact tcctttaccc
    31621 acttattgag aaagcggggt tggcataata tggcgaaaaa cttgcaagaa atgcgtgaac
    31681 gtgtctgtgc ggctaacaag tctctaccac aacacggtct tgtcaaattt acgtggggca
    31741 atgtctctga agtttgtcgt gaattaggac gtattgtcat caagccttct ggggtggact
    31801 acgacctctt gacaccagaa aacatggttg ttacagacct tgatgggaat gtggtcgaag
    31861 gtgatttgaa cccttcttca gatctgccaa cccatgtgga actttataaa gcttggccag
    31921 aagtgggcgg tattgtccac actcactcca cagaagctgt tggttgggct caggcaggtc
    31981 gtgatattcc gttttatggg actacgcatg ctgactactt ctatggacca gttccgtgtg
    32041 ctcgttctct cacaaaagca gaagttgatg gggcttacga gcaagaaact ggcaacgtga
    32101 ttttagaaga gtttagcaag cgaggcttag atcctatggc ggttccagga atcgtggttc
    32161 gcaatcatgg cccatttacg tgggggaaaa ccccagagca agccgtttac cactcggttg
    32221 tcttagaaga agtggctagg atgaatcgct tgacagaaca aatcaatcca cgcgtagagc
    32281 cagcgccacg atacattatg gataagcatt atttgcgtaa acatggccca aatgcttatt
    32341 acggtcaaaa atgaggtcac gatatggctt attagcacag gttctcacta gaagccagaa
    32401 agttcaaggt tttgttaatc attgtttggg caagtccttt tgacttgtcc ttttcgatga
    32461 actatttaaa cgccaatgag cacaaactgg atgaatcgta tgttgtggag ttcttagaga
    32521 ccagatataa ggtgttggtg ttggatcagt tgaggtgatt agctcgaatc atattaagcg
    32581 aatcttatta tggtgagttt ttcaaaactt ggctgcgcag tcactgaaat catgataaaa
    32641 taagcaagga tggattttca gagaggaggt ttgtaaacat gatgatttta gataaaaaga
    32701 gttatgactt gctgtcttat ttacttaaac tagaaacacc tgagacagtt atggccattt
    32761 ctcatgcttt aaatcagtca cgtcgtaaag tttattacca gcttgacaaa attaatcagg
    32821 ctttgccaaa gggagtagat cagattatct cttatcctag acttggcatt cttttaacag
    32881 ctgatcaaaa agctgcttgt cgcttattat tagaagaagt gactgattat aattatgtca
    32941 tgaaaagtga tgagcgcaga cgcctatcct cgatttatat tgcggtttcc actgagcgcg
    33001 tgaccattga taaactgatg cagatcaatg atgtgtctcg taacacgatt ttaaatgacc
    33061 tgactgagtt aagggaagaa ctggaagaca aacaatacaa gattcagctt catgcgacaa
    33121 aggctagggg atattatttt ggttgtcatc caatggctct tattcaatac ctgtacaaat
    33181 tgttagtgga cgtttaccaa ggtggcaata ctagctttat tgatatattc aaccgtaaac
    33241 tctcagagat acaaggctta tcggtttatt tttctaaaga tattttgacc tattttcatg
    33301 agtatctctt tttatctcaa gctagtttag gaaagacaat taatacccaa gatagccaat
    33361 tcatgttgca gattttacct ttcatgcttt taagttatcg taatatgcgt cttgattcag
    33421 aaacaaagtc tgctcttaaa caagaatttc atttgatttg gaaacgtaaa gaatatcaca
    33481 ttgctcagga tttagctaga gagctttacc ataattttaa gcttcatttg gatgatatcg
    33541 aagttagtat ggtagcgatg ttgatgttgt cttttcgaaa agatcaagat catcacgtgg
    33601 aaagtcaaga ttatgacgac atgcgagcaa ccattagtca ctttattgac cagttggaga
    33661 gccgttatca gttacatttc acgcataaac aggatttgct gaagcagttg acgacccact
    33721 gtaaggcgct cgtttaccgt aaagcttatg ggattttttt agttaatccc ttaacagacc
    33781 atattaaaga aaaatacgaa gaattgtttg ctatgactca atcttgtgcc actattttgg
    33841 aacaagattg gacgattagc ttgacagacg atgatattgc ctacttgact atccatcttg
    33901 ggggagagct gcgtcacaat aatacagagc aagaaaagac caaactcgtt attgtttcag
    33961 atgatgggat aggcattcaa aagttattgt tcaaacagtg tcaaaggtat ctagcaaatg
    34021 gtcagattga agctgttttt acgacagaac agtatcagag tgtttacgac cttttagcag
    34081 tcgatatgat tgttgctact acagatacgc taaagactaa aatcccgatg cttattgtta
    34141 accctatttt aagtgacgat gatatcatca aattgatacg cttttcaaag caaggacgct
    34201 tgagtgagca ctcccgcttt tctacagagc tcacaaaagc tattgaagct gttgtaaaag
    34261 atgaatcaga tcgttatgcc ttggtttcta aaattgaaaa actcattcat cgcgagctgc
    34321 tatgagtaag tgttaagtcg tcaaagtacc tcagttttct atgacataga aaaatataac
    34381 tgaggttttt aatatagctt tgaatacagt attgatgcaa gatacagtgt gcaaaatgag
    34441 acagtattag actgagctaa tgaaaaagtc agtccacttt tgaacacaga tctgtgtgac
    34501 acttggtctt tttatcattg acaagaaagt ctatacttaa agtatagaaa cgaagaacaa
    34561 attcggagga aaccctaatg actaaagtac aagatattac acgtgaatca tggatcctta
    34621 acacgtttcc agaatgggga acatggttaa atgaagaaat cgagcaagaa gtcgtgccag
    34681 ctgacaactt tgcgatgtgg tggttaggaa actgtggtat ctggattaag acaccaggcg
    34741 gtgctaatgt tgtcatggac ttatggtcaa atcgtgggaa agcgaccaag caagttaagg
    34801 atatggttcg tggacatcaa atggctaata tggcaggagc tcgtaaatta cagcccaatc
    34861 ttagggccca accaatggtg attgatccat ttatgattaa tgagttggat tattatttgg
    34921 tttctcatta tcatagtgac cacatcgata tcaatactgc ggcagccatt atcaataacc
    34981 caaaacttaa tcatgttaaa tttgtaggac cttatgaatg tggtgaagtt tggaaaaatt
    35041 ggggtgtgcc aaaagatcgt attatgatct taaagccagg tgatagcttt gaattcaaag
    35101 atattaagat caccgctgta gagtcgtttg accgcacctg tctggtaact ctcccaatac
    35161 aaggagctga tgctcaagat ggtgatttag caggtttagc tatcacagat gatgacatgg
    35221 ctcgcaaagc tgttaactat atttttgaaa caccaggtgg taccatctat catggtgcgg
    35281 attctcattt ttcgaactac tttgctaaac atggacgtga ttatgatatt gacgttgtgt
    35341 taaataacta tggggaaaac ccaattggaa ttcaagacaa gatgacttct gttgaccttc
    35401 ttcgtatggc agaaaatctc cgtgctaaag tcgttatccc agttcattat gacatttggt
    35461 caaactttat ggcatcaaca gatgaaatcc ttgaactgtg gaaaatgcgc aaagaacgtc
    35521 tccaatacga tttccatccc tttatctggg aggttggtgg caagtacacc tatccacaag
    35581 accaaaatcg tatcgaatac catcatcctc gtggatttga tgactgcttc ttagaagatt
    35641 ctaatatcca atttaaagct ttactataag caacacccta ctcacctaag atgatagtgc
    35701 ctgattaagc taactcgctt cataggacac tgtcttaggt gagttttttt ggagagatga
    35761 gcaaatgttt aagagtcacc gtggtcgtta tttacgatgg ccgcaaaaga gatattatag
    35821 gtaaaagaag gatttctaat gtaacaatag cctaatcagc aaagaaatag ccatcagttg
    35881 tgataaaaaa ttgtactggt taaaagacca tacctaaaag aatgaaagcg atcacctatc
    35941 attgtggcat taccagatcg agttattgtc cttgacaaaa agtgaccaca cgtgataata
    36001 tagttatgtt ctgtttcggc agattctttt tttaagtaaa aaagtgacca cacgtttatt
    36061 aattgtgtag agctactttg ttaaatattg gctaatattt cgccaaagct atgtcattat
    36121 ttcacatgat aaacgattac ctaggagaaa aaacgaatgg aaacaatttt agaagtcaaa
    36181 catctcagta aaatttttgg caaaaaacaa aaagcagctc ttgagatggt aaagactggc
    36241 aaaaataaga gtgagatttt taagaaaaca ggcgctactg taggtgtcta tgacgctagt
    36301 tttgaggtca aaaaaggtga aatctttgtt attatggggc tatcaggaag tgggaaatca
    36361 acccttgtcc gcatgctaaa tcgtttgatt gaaccttcag caggatctat cttgctggaa
    36421 ggtaaagaca tctcaaccat gtcagcagat cagctgcgtg aggtgcgccg ccatgacatt
    36481 aacatggttt tccaaagctt tgccctcttt cctcataaaa ccattttgga aaataccgaa
    36541 tttggtttgg aattacgtgg cgttcccaaa gaagaacgcc agcgattggc agaaaaagcc
    36601 cttgataatt caggcctatt agattttaaa gaccagtacc caaaccaact atctggtggg
    36661 atgcagcagc gtgtcggcct agcccgtgcg ctagctaata gccctaaaat tctcttaatg
    36721 gacgaggcat tctcagcgct tgatcctttg attcgtcgtg agatgcaaga tgaattactt
    36781 gatttgcaag acagcatgaa acaaaccatc atctttatca gtcatgactt gaatgaagcc
    36841 ttgcggattg gtgatcggat tgctttgatg aaagacggac aaattatgca aattggtact
    36901 ggtgaggaaa tcttgactaa cccagccaat gactttgtgc gtgaattcgt tgaggatgtg
    36961 gaccgttcta aagtcttgac agcacaaaat atcatgatca aaccgttaac aactactgtt
    37021 gaattagatg gacctcaagt tgccttgaac cgtatgcaca acgaagaggt atctatgttg
    37081 atggcgacga atcgccgccg ccaattagtc ggcagtttga cggccgatgc cgctatagag
    37141 gcgcgcaaaa aagggttacc gctatcagaa gtgattgatc gcgatgtgag aactgtctca
    37201 aaagatacta ttattacaga tattttgcct cttatctatg attcatctgc tccgattgca
    37261 gtgacagatg ataataatcg tctgttaggt gtcattattc gaggacgagt gattgaagcc
    37321 ttggctaata tctcagacga agaccttaac taaaaggagt gatgcatttt ggaaacgata
    37381 ttacaaacaa aattaccagt agctcaactg gtggagcagc taacggagtg gctaactaaa
    37441 actttctcag gtctatttga tattatgcaa gtagttggta gctttttgat ggattggatg
    37501 accaaaacac ttttatttat ccacccgtta ctttttattg ttctagtaac agcagggatg
    37561 tttttccttg ctaagaaaaa atggcctttg ccaaccttta ccttgttagg attgctcttt
    37621 atttacaatc aagggttatg gaagcagcta atgaatacct ttactctggt gttagtagct
    37681 agtttgattt cggtacttat tggtattccg ctggggattt ggatggctaa aaatgccact
    37741 gtccgtcaga ttgtgaatcc tattttggac tttatgcaaa ccatgccggc ttttgtttac
    37801 ttgattcctg ccgttgcctt ttttggtatt ggtatggtgc ctggagtatt tgcttcagtc
    37861 atctttgcct tgcccccaac agttcgtttt acaaatttgg ccattcgtga cattccaaca
    37921 gaattgattg aggcctcaga tgcctttggg tcaactggga aacaaaagct ctttaaagtt
    37981 gaattgcctt tagctaaaaa tactattatg gcaggtgtta atcagaccat gatgttagcc
    38041 ctttccatgg tagtaacagg ctccatgatt ggtgctcctg gtttaggacg tgaagtcttg
    38101 tcagcccttc aacacgctga tattggtagt ggatttgtca gtggtcttgc cttggttatc
    38161 ttggcgattg ttcttgaccg tatgacacag ttgttcaata gcaaacctca agaaaaagcc
    38221 aaagctggca aaaccaataa atggattggt ctagcagccc ttgctgtctt tttgatagct
    38281 gctttaggac gcggtattat gactatggct tctggtatgg cagataaggg tgaaactgtt
    38341 aacatcgctt acgtgcagtg ggattctgag gttgcctcaa ctcatgtgat tgcagaagtt
    38401 ttgaaaaacg aaggttacca tgtaaccttg acaccgcttg acaatgctgt catgtggcaa
    38461 accgttgcaa atgggaacgc tgatttttca actagtgctt ggttgccagt cacccatggc
    38521 caacaatatc agaaatacaa gtcaaaatta gatgatttag gacctaacct aaaagggacc
    38581 aaacttggtt tagctgttcc taaatacatg acagatgtta atagtattga ggatttaagc
    38641 aaacaagccg atcaaaaaat cacagggatc gaaccaggtg caggtatcat ggcagcagcc
    38701 aaaaagactc taaaagagta tcataatcta tctagttggg agttagtggc ggcatcaaca
    38761 ggagccatga cgacttcctt ggatcaagcg atcaagaaaa aagatcctat tgtcgtgaca
    38821 gcttggtcac cacactggat gtttgccaag tatgacttga agtaccttaa agatcctaaa
    38881 gaaacctttg gctcgactga gaacatcaat accattgctc gtaaaggctt gaaaaaagat
    38941 cttccaaatg tctacaaaat cattgataag ttccactgga cccaaaaaga tatggaagca
    39001 gtcatgcttg acatcaataa aggcatgagt ccagaagctg ccgccaaaaa atgggtagag
    39061 gcaaacaaat ctaaagtatc aagttggaca aaataagtgc ttttgtctaa atagcaaata
    39121 cagaagtcta ggaattttcc taggcttttt aggttaacca tttacttatc agcaaaacca
    39181 atggcttaaa ggagaaaagc ccgtccctct gtgttataat aaggctatgg aaaacaagaa
    39241 taaattacta ttaattgatg gctcctctgt tgcctttcgt gccttttttg ccctttacaa
    39301 tcaaattgac cgctttaaaa atcatagcgg tctccatacc aatgccattt atggctttca
    39361 tttgatgcta gaccacatga tgaaaagagt ccggcctact cacgtgctgg tggcttttga
    39421 tgcgggcaaa accaccttcc gaacagaaat gtatgccgat tataaggcgg gtcgcgctaa
    39481 aaccccagat gaatttcgtg agcagttttc ttatattcgt gagatgttga cagcccttgg
    39541 gattgcttat tatgagttgg aacactacga agcagacgat attatcggga cgcttgataa
    39601 aatggcggag cgcacagaag tgccttttga tgtgaccatt gtcagtggtg ataaagattt
    39661 gatccagtta actgacgaaa ataccgtggt tgaaatttct aaaaaagggg ttgccgaatt
    39721 tgaagaattc acaccggctt acctcatgga aaaaatgggc ttgacgccaa accagtttat
    39781 tgacctcaaa gccttgatgg gagataaatc ggataacatt cctggagtga caaaaattgg
    39841 agaaaaaaca ggccttaagc tccttcatga atttggtagt ttggaaggca tttacgagca
    39901 tattgatgga ttcaagacct caaaaatgaa agaaaacttg atcaatgacc gtgaccaagc
    39961 tttcctatct aaaacgctcg ccactatcaa tacagcatct cctatcacaa ttggcttaga
    40021 tgatattgtc tacaatggtc cagatgttgc tagcctctct caattttacg atgagatgga
    40081 ttttgtgcaa ctcaagaaag gtcttgctag ccagatgcct caagagccag tagctgtcat
    40141 ttcctatcaa gaagtgacca atgtgtctgc tgatttgttt agcgcagaag acatcttcta
    40201 ctttgaaacg cttcgggata attaccatcg tgaggccatc attggttttg cttggggaca
    40261 tggtgagcag atttacgcat ccacagacct tagcctatta gcgacggata gttttaagca
    40321 ggtcttccaa aaacccattg ccacttatga ttttaaacgt agcaaggtct tgttgagcca
    40381 cttaggaatt gagttggtag cacctagcta tgatgctaga ctagctaatt accttctgtc
    40441 aactgtagaa gacaatgagc ttagcaccat tgcccgcatt tttactgaca tctctttgga
    40501 agaagacgat acggtctatg gtaaaggtgc taaacgggct gttcctgata aagacgtctt
    40561 actagaacac ttagctcgta aggtcaaagt attattagat agcaagtctc aaatgctaga
    40621 caagctcact gcccatgagc aactagacct ctatcagaat attgaattgc ctttggctaa
    40681 tgtccttgct aaaatggaaa ttgaagggat caaggttaat agggcgactt tgcaagatat
    40741 ggcagagcaa aacaaggtca ttattgaagc gctcacccaa gagatttatg atatggctgg
    40801 ccaagaattt aacattaatt cgcctaaaca gctgggcagt atcttgtttg agaagatgca
    40861 actacctctt gagatgacta agaagaccaa aacaggttat tctacagctg ttgatgtttt
    40921 ggaacgtttg gcaccgattg ctccaattgt ggctaaaatt ttggattacc gccagattac
    40981 taaattgcaa tccacctatg tgattggttt acaggattat attctggctg atggcaagat
    41041 tcatacccgc tatgtgcagg atctcaccca gacagggcgt ttgtctagtg tggaccctaa
    41101 tcttcaaaac attcccattc gtttggaaca ggggcgtttg attcgtaagg cctttacccc
    41161 atctcatgaa gatgccgtct tgttgagttc agattactca cagattgaat tgcgcgtgct
    41221 agcacatatt tcaggggatg agcatttgat tgctgccttt aatgaagggg cagacattca
    41281 tacctcaacg gctatgcgtg tgtttgggat tgacaaggca gcagatgtta ctgccaatga
    41341 ccgccgtaat gccaaggctg ttaactttgg aattgtgtat gggatttctg attttggttt
    41401 gtcaaataac cttggtatca cccgtaaaca gtccaagtct tacattgata cctattttga
    41461 acgctatcct gggattaaag cctatatgga aaatgtggtg cgtgaggcta aagacaaggg
    41521 ctatgtagag accctcttta agcgtcgccg tgaattaccg gatatcaact ctcgtaattt
    41581 caacgttcgc tcttttgcgg agcgtacagc catcaactcg cccattcaag gaagcgcagc
    41641 tgatattctc aaaatcgcta tgattaatct ggataaggcc ttgcaagcag gtggttttag
    41701 agctaaaatg ctgcttcaag tgcatgatga aatcgttttg gaagtgccaa atgatgagtt
    41761 gactgctata aaaaaacttg tcaaagacac catggaagct gctgttgatt tagctgttcc
    41821 tttatgtgtc gatgaaagta ctgggcagag ctggtacgaa gccaaataag ttttcttact
    41881 tgttatttct cattttgaca acttatgtta taataaacta aaaaattgta ggagaagtca
    41941 tgaagctgct ttgagctttt tgacggataa ggttaaaagt gattgaagcc atgctttagg
    42001 agttatcctg ctatattgaa cagctgtaga ggttattaaa tagatagggg aacaagtgat
    42061 ttattcgttt caaaatccct cagaggatat cctaaaagct tacttggagt cagccaaaac
    42121 cattgcggtt gttggtcttt ctgaccgcaa agacacagca gcttatggtg tcgccaaatt
    42181 tatgcaggct atggattatc ggatcattcc agtcaatccc aaattagcag gtcagcttat
    42241 tttgggagag aaggtctatg ccagtatcaa ggcaattccc tttgaggttg acattgtaga
    42301 tgtctttcgc agaagtgagt ttttaccaga agttgccaga gactttttag caggtcaggc
    42361 caaagtcttt tgggcgcaat taggccttga aaaccaagaa gtacaaacca ttttacggtc
    42421 ggcaggcaaa gaggctattg tgatgaatcg ctgcctaaaa atagactatt tgcaattagt
    42481 cgctaaacca gaatgcatca caaatctttg aaactagaga cgtgcgtgat aataaaggaa
    42541 aaaaggatag gagtagcagc ttatggacat tcattcacat cagcaagccc tagatgccta
    42601 tgaaaatgtc ctagagcatc tacgggaaaa acatattcga attacagaga cacgtaaagc
    42661 cattatttct tacatgattc agtccacaga acaccctagt gccgataaga tttaccgtga
    42721 cttacagcct aactttccta acatgagcct tgccacagtc tataataatt tgaaagtttt
    42781 agttgatgaa ggttttgtct cagagttgaa aatcagcaat gatttaacca cctactatga
    42841 ctttatgggc catcaacacg tcaatgtagt ttgtgaaata tgtgggaaga ttgctgactt
    42901 tatggatgtt gatgttatgg atattgccaa agaagcccat gagcaaacag gttataaggt
    42961 cacgcgtatc ccagttattg cttacgggat ttgtccggat tgccaagcaa aagaccaatc
    43021 tgatttttag agttaaccct taaataaagg cattataaat atagtgcctt tacaaatcaa
    43081 aaacccccag ttcaatacca gacctttgtt gcttatgtta aagagttatt tgctggggat
    43141 cataaggaat taaaggaaag aaccatcaat catctcctct atttgtttgt tcctcacctg
    43201 ggaaaatctc tttcttacaa atcaatcgcg tcgtcgcttg aaactattag tggttgagag
    43261 aagttataac aatgttggca acttcttaaa agagtatttt ggaggatttt ttgagattat
    43321 caactttgat gacttgaggc tgaccatggt tgatatggtg tcactgtctt cagagtatga
    43381 cgtaattgtg acagatatga ttcttgagca aacaatggac tccgaaattt tatttttcaa
    43441 tcagatggcc cctagtgtgg tggcgaatag attgacagac atgtaactaa acaatgataa
    43501 tatctgtgag agcatcggaa tttaaaaaat gagcgataaa taagattaag aataagttag
    43561 acttacaagg agtagagtct attaccttga aaaagaaaag caaggtttaa ttattcaact
    43621 tgaaaaatta aaaaacaaaa agctcgaaaa agagattctt agtga
//
LOCUS       DAUGHY010000010        41286 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.010, whole genome shotgun sequence.
ACCESSION   DAUGHY010000010 DAUGHY010000000
VERSION     DAUGHY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41286
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.010"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            32..805
                     /locus_tag="VH752_000513"
     CDS             32..805
                     /locus_tag="VH752_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ8288887.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
                     AINNLETLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTDIIEKYKKFSKIIIADK"
     gene            1455..1742
                     /locus_tag="VH752_000514"
     CDS             1455..1742
                     /locus_tag="VH752_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible protein J"
                     /protein_id="HEQ8288888.1"
                     /translation="MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNV
                     VVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
     gene            1732..2067
                     /locus_tag="VH752_000515"
     CDS             1732..2067
                     /locus_tag="VH752_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ8288889.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISD
                     IEKLEVFPEVGFDADEKYGSKISHYHSTKGYTLSKDYIVLYHIEGEENRVAIDYLLPT
                     QSDYIKLFK"
     gene            2219..2689
                     /locus_tag="VH752_000516"
     CDS             2219..2689
                     /locus_tag="VH752_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEQ8288890.1"
                     /translation="MEEFTKPTHKTYSEIFEKWYQAYRDTVEPTTASRTLNLFRLHIL
                     PVMGELPINKTSPLDCQNFITDKAKTFKNIKQIKSYTEKVFDFAIKMKLLKHNPMAEI
                     IMPKRKKTRIENYWTVQELQEFLVIILQKEPYKHYALFRLLAYSGLKERRAMRS"
     gene            2652..3014
                     /locus_tag="VH752_000517"
     CDS             2652..3014
                     /locus_tag="VH752_000517"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEQ8288891.1"
                     /translation="MAALRKGELCALKWADIDFQSETLSVNKSLGRLDGQAIKKSTKN
                     DFSVRKIKLDSETISILQEWKAISQKEKTELAVAPLSIESDSIFTYCTREGSIEPLHT
                     DYINNILSKVIKQHSLKK"
     gene            3050..3199
                     /locus_tag="VH752_000518"
     CDS             3050..3199
                     /locus_tag="VH752_000518"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288892.1"
                     /translation="MIEIGVDPVNTAKRLGHASSQMTLDTYSHATAVGEDKSIKQFAN
                     CLKNN"
     gene            complement(3319..3711)
                     /gene="rpsI"
                     /locus_tag="VH752_000519"
     CDS             complement(3319..3711)
                     /gene="rpsI"
                     /locus_tag="VH752_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEQ8288893.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3732..4178)
                     /gene="rplM"
                     /locus_tag="VH752_000520"
     CDS             complement(3732..4178)
                     /gene="rplM"
                     /locus_tag="VH752_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEQ8288894.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(4396..4602)
                     /locus_tag="VH752_000521"
     CDS             complement(4396..4602)
                     /locus_tag="VH752_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8288895.1"
                     /translation="MKNLKLKAARAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEADDSR"
     gene            complement(4599..5105)
                     /locus_tag="VH752_000522"
     CDS             complement(4599..5105)
                     /locus_tag="VH752_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288896.1"
                     /translation="MFDSKQNLAKSQWGFIVITAVMDLVVIVATLLAKTSFQRYLVGG
                     VAFVLTSFLILLVWGLKTAKKLQTRLDSKEVLDSKVRDDQKLPKYDERQKQILLKGYT
                     IGFWFMIVVIWLSLFISRFTEGLVSASFLFTLALWGGLAVQTTYCNLSMGASIFYRHY
                     LDAKEADE"
     gene            complement(5241..6101)
                     /locus_tag="VH752_000523"
     CDS             complement(5241..6101)
                     /locus_tag="VH752_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ8288897.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHARNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            complement(6198..6716)
                     /locus_tag="VH752_000524"
     CDS             complement(6198..6716)
                     /locus_tag="VH752_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEQ8288898.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKTNQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     IQLKDFMSQLDR"
     gene            complement(6720..7466)
                     /gene="rlmB"
                     /locus_tag="VH752_000525"
     CDS             complement(6720..7466)
                     /gene="rlmB"
                     /locus_tag="VH752_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEQ8288899.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKSRGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
     gene            complement(7513..8310)
                     /locus_tag="VH752_000526"
     CDS             complement(7513..8310)
                     /locus_tag="VH752_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288900.1"
                     /translation="MTTMQKTVSLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQVSVEASMRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMAAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAT
                     TNSVDVDTKAQTNQSANQEIDSTSNPFRSATNHRSTSLKRSTKNEKLTPTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLLGLVVSLAAGLFIAIQKVSRRK"
     gene            complement(8506..8919)
                     /locus_tag="VH752_000527"
     CDS             complement(8506..8919)
                     /locus_tag="VH752_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEQ8288901.1"
                     /translation="MTNPVDVNLINGIALAFEGDAVYSYYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAHLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            complement(8912..10255)
                     /locus_tag="VH752_000528"
     CDS             complement(8912..10255)
                     /locus_tag="VH752_000528"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEQ8288902.1"
                     /translation="MIKIYDTMTRSLRKFVPLTENTVNMYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDCFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVTVDSEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKAIHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLEVDIEALIAKRQEARANRDFATADAIRDQLAAQGIKLLDTKDGVRWLRD"
     gene            complement(10283..10513)
                     /locus_tag="VH752_000529"
     CDS             complement(10283..10513)
                     /locus_tag="VH752_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288903.1"
                     /translation="MNKIRVGLYVIMSLSILVFSITLVASLIFLLRQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            complement(10992..11573)
                     /gene="cysE"
                     /locus_tag="VH752_000530"
     CDS             complement(10992..11573)
                     /gene="cysE"
                     /locus_tag="VH752_000530"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEQ8288904.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTIRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            complement(11582..12334)
                     /locus_tag="VH752_000531"
     CDS             complement(11582..12334)
                     /locus_tag="VH752_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEQ8288905.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSLALVNAFHNFPVWAAKEPYV
                     IEVEGVKVTPVFTDKEDMARFKEEQKSAQSQYWLERSALAVLEEVITSGAAGLVFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYTTVLNTLMSDDNVAADTMEKVYLVPAFVYPKDNNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            complement(12327..14459)
                     /gene="pnp"
                     /locus_tag="VH752_000532"
     CDS             complement(12327..14459)
                     /gene="pnp"
                     /locus_tag="VH752_000532"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEQ8288906.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGDSTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIVAVVGKEKAEVELLQVDADLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSILTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAINRTKEIIAGLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKI
                     DEKGRVDASMKALIPRPPKPEKKEEKHD"
     gene            complement(14740..15471)
                     /locus_tag="VH752_000533"
     CDS             complement(14740..15471)
                     /locus_tag="VH752_000533"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEQ8288907.1"
                     /translation="MMTKKLNVKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEVFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCSVIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTAGVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICLQKEGVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLETVQMFLKDSTSLGFKILEDDNH"
     gene            complement(15481..16860)
                     /locus_tag="VH752_000534"
     CDS             complement(15481..16860)
                     /locus_tag="VH752_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEQ8288908.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLAAGAGVIVTNLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     VGLLLNLTFARFTRFKYIFLTGHHSFFMACLLSAVLGAVGFKGSLLIILGGFLLGAWS
                     AISPAIGQQYTLKVTDGDEIAMGHFGSLGYYLSAWVGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVIFYLVATVASVLRNASVTEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAGIFGNSTGGRRGAMIGAFANGLLLAFLPAMLLP
                     VLGKLGFSNTTFGDVDFGVLGILLGRLGTSIGQIGIYLVVAVLAIVLVLPNFLSKSQE
                     AINNVSEEI"
     gene            complement(16887..17171)
                     /locus_tag="VH752_000535"
     CDS             complement(16887..17171)
                     /locus_tag="VH752_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ8288909.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKGADLFVTVKEFKDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            complement(17164..19224)
                     /locus_tag="VH752_000536"
     CDS             complement(17164..19224)
                     /locus_tag="VH752_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ8288910.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESILKTSKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPPDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVTSGPWVFSYLLHELGLPDQKKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRDVRGEAVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLDLVFQQPIYDIMEEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKEQIKQDYESLFYLVKRSLSPLEKQLGKSVNEDEVAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     AKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLIGNHLTIEKGGLGLLDLLTADFIRQADAVSDWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLAPKVAVPHAAPEKGTRQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            complement(19576..19845)
                     /gene="rpsO"
                     /locus_tag="VH752_000537"
     CDS             complement(19576..19845)
                     /gene="rpsO"
                     /locus_tag="VH752_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEQ8288911.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNSHIK
                     EHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(20021..20221)
                     /locus_tag="VH752_000538"
     CDS             complement(20021..20221)
                     /locus_tag="VH752_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288912.1"
                     /translation="MTMLGYRLSAEVLGSAILPLLAGYLIRQAIAQVYSDLKTASWLF
                     GLASSVAYGGLVKAKRAHSTTR"
     gene            20347..20604
                     /locus_tag="VH752_000539"
     CDS             20347..20604
                     /locus_tag="VH752_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ8288913.1"
                     /translation="MTQNVCQQNITYTVKERFASYTLEAQANQEVHLNLTLLANRFGT
                     SDRHLKHVLKQPIFQRIIERICFKTCRIADLDALTQLSVNN"
     gene            20731..21345
                     /locus_tag="VH752_000540"
     CDS             20731..21345
                     /locus_tag="VH752_000540"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ8288914.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD"
     gene            complement(21378..21920)
                     /locus_tag="VH752_000541"
     CDS             complement(21378..21920)
                     /locus_tag="VH752_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ8288915.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVSYLNWKDVPV
                     LNQDIEANAPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKEIKANLLSQAEALLAAI"
     gene            complement(22052..22480)
                     /locus_tag="VH752_000542"
     CDS             complement(22052..22480)
                     /locus_tag="VH752_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ8288916.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     TRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
     gene            complement(22590..26987)
                     /locus_tag="VH752_000543"
     CDS             complement(22590..26987)
                     /locus_tag="VH752_000543"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEQ8288917.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLIRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYEDDKLIIAAPGFVNNDHFRNNHLPNLVKQLEAFGFGTLTID
                     MVSDQEMTEHLTKDFVSSRQALVKKAVQDNLEAQKSLEAMMPPVEEATPAPKFDYKER
                     AAKRQAGFEKATITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVHHDRKDLM
                     PEGQKRVELHAHTNMSTMDALPTVESLIDTAAKWGHKAVAITDHANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYDPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQGAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHDLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLRDAREKHGIKNLLQLNTDLVAEDSYKKARIKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLAKYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRAKKPVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGEGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVQNTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGDEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     ILGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHHPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEMSILGNMPEDNQLSLFDDFF"
     gene            complement(27241..29097)
                     /locus_tag="VH752_000544"
     CDS             complement(27241..29097)
                     /locus_tag="VH752_000544"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEQ8288918.1"
                     /translation="MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPART
                     DLDRWVVLDKSIASMDDIPKEVLEEIKAELAAWMISGEDTIAYSTESSYAANLEMATN
                     EYKPSSKVAAEDALAEVETPHCKTIDEVAAFLSVDETQTIKTLLFVADNEPVVALLVG
                     NDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLT
                     NAVAGANKDGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFK
                     LGTRYSDSMGATILDENGRAVPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGDYRY
                     AWGVNFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLTAKGYDVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKAAEGIVEIKIKATGASIEVNAENLIETLEILTKEH"
     gene            complement(29295..30554)
                     /gene="rseP"
                     /locus_tag="VH752_000545"
     CDS             complement(29295..30554)
                     /gene="rseP"
                     /locus_tag="VH752_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEQ8288919.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPASLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLEETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMPDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHA
                     KTYTIGVKASLKTGFKDKLLGGLELAWSGAFTILNALKGLIIGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLSLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
     gene            complement(30627..31421)
                     /locus_tag="VH752_000546"
     CDS             complement(30627..31421)
                     /locus_tag="VH752_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEQ8288920.1"
                     /translation="MKERVVWGGVAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPTVSPNKTIEGSLGGIACAVLVSFIFMVIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            complement(31434..32183)
                     /locus_tag="VH752_000547"
     CDS             complement(31434..32183)
                     /locus_tag="VH752_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEQ8288921.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVRFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNHRQRRFGKV"
     gene            complement(32402..32767)
                     /gene="yajC"
                     /locus_tag="VH752_000548"
     CDS             complement(32402..32767)
                     /gene="yajC"
                     /locus_tag="VH752_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEQ8288922.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELSAIKRIVTKATTETTLVETEETSVVN
                     EPAVETVDSTDTDSAIESH"
     gene            complement(32883..33257)
                     /locus_tag="VH752_000549"
     CDS             complement(32883..33257)
                     /locus_tag="VH752_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEQ8288923.1"
                     /translation="MRPKEKETSMTFEEIVANFIPSSIAEVTSAIASGKDMIVFLGRS
                     SCPYCRRFAPKLAQVATDNQKEVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQ
                     HQRAVCDSSLTPDDILAFLTRE"
     gene            complement(33372..36869)
                     /locus_tag="VH752_000550"
     CDS             complement(33372..36869)
                     /locus_tag="VH752_000550"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEQ8288924.1"
                     /translation="MKKKVNQGSKRYQYLLKKWGIGFVIAATGTVMLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSGDGFQSSSKEAKPVLDSSSANPASIAEHHLRMHFKT
                     LPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGEHLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKTPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDKDTSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADSVKVVVYDKQDQTKVLGQADL
                     TKSDKGVWRAHLTSDSVKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATAT
                     DDIKTAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     YGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTKVS
                     DDKVPNKATLIDAVAKYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLGAQVLADAEQAGVTAIAKPKGVQFTKEGLTIDGLTALVLKV
                     SSKTANPSQQKSQTGNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLA
                     AGVALLLLAISLLMKRQKD"
     gene            complement(37067..38680)
                     /locus_tag="VH752_000551"
     CDS             complement(37067..38680)
                     /locus_tag="VH752_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEQ8288925.1"
                     /translation="MKKHWWHKATIYQIYPRSFKDTNGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDNASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDLEITGNGPRLHDYL
                     KEMNQATFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAKELDVPALKAIFSKWQTELKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVNDIESLNYAKEAMGNGVSAARVMDS
                     IRKVGRDNARTPMQWSKDTHAGFSKAQETWLPVNPNYQDINVADALADPDSIFYTYQQ
                     LIALRKEQDWLVEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDQEQVFAKDLAGAE
                     VVITNTDVDKVLETKHLQPWDAFCLKLKA"
     gene            complement(38809..39942)
                     /gene="ugpC"
                     /locus_tag="VH752_000552"
     CDS             complement(38809..39942)
                     /gene="ugpC"
                     /locus_tag="VH752_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEQ8288926.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYSLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLAIAIPEG
                     QAKMLEAAGYKGKKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            complement(40240..41088)
                     /locus_tag="VH752_000553"
     CDS             complement(40240..41088)
                     /locus_tag="VH752_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEQ8288927.1"
                     /translation="MELKDYFPEMQVGPHPLGDKEWVSVKEGDQYVHFPKSCLSEKER
                     LLLEVGLDQCEVLQPLGSPWQRYLLDHQGNPPQLFETSQFIYLNHQQALPADLVELLQ
                     QMIAGLEVILPISATQTAFLCRQAVSIKVLRWLEDLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFQQFLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLHLKQLDDLAFAH
                     LFLLKY"
BASE COUNT        12243 a         9089 c         7764 g        12190 t
ORIGIN      
        1 ttatacccta agacactgat aggagataag aatgaaaaag aaagagcgtc atgaaaaaat
       61 tctcgatatc ttaaaagttg acggttttat aaaagtaaaa gatattatcg atgaaatgaa
      121 catctcagat atgaccgcta gacgagatct cgatacattg gcagataaag gattacttat
      181 caggacccat ggtggtgctc aatatttgga ttattcttca gcaaaggacg aaggtcacga
      241 gaaaacacac actgaaaaga aggtcttaca gactaccgaa aaaaaacaaa ttgcacaaag
      301 agctaaaaac attattaaag atggcgaaac tgtctttata ggtccaggga caacattaga
      361 gcatctagct gtcgaattaa aaaatagaaa tatcagggtc attacaaata gccttcctgt
      421 ttttttaatt ttgcacccga gcactactat tgatttgatt ttaattggtg gtgaatgtcg
      481 tgaaattact ggagcttttg ttggtgctat tgccattaat aacctagaaa cattgacatt
      541 ttcaaaagct tttgtcagcg caaatgcagt ttacaataat tctattgcga cgtacagtga
      601 tctcgaagga gaagttcaaa gggttgcatt gaataatgct gttgagaaaa tccttctagt
      661 tgatagtacc aagtttgaaa gttatgactt ctataatttc taccaactag atcaagtgga
      721 cttaattgtg actgataaaa atgtgacaac tgatatcata gaaaagtaca agaaattctc
      781 aaaaatcata atcgctgaca agtaaaagac ctactcatat taaaaagcac ttcagggttt
      841 agatatatcc cctgtcaagt aaacagggag catatctgaa agtagtgttt gtgttataaa
      901 gaagtttctt gttaacttat tttctggttt ttttcagact tatcagtctt gtactgttcc
      961 tttttctgat gaataacgtg gaaataggca gacagctata tatttcacct ctagaatggt
     1021 tgccattact gtcatcaaga catgcttgtt ctttatcgta tggagtgaag ctttctttta
     1081 gacatcaaaa tacctcttaa gtagattttg gttcgttacc atgtctactt aagaggtgtc
     1141 ttcaaagcgc tttaaaacgt tgatataaca gtatattaac gttttgagaa ttgtgaagct
     1201 ttacgagctt ttttaagacc tggtttttgg ttcactccat tatttaaagt caaatccctt
     1261 taaaatcaat gtttctaaaa tgaaaaatca ctaaaaatct tatttataga caaatactta
     1321 aacctaaacg gtatcaaaaa taagtttcgg agcttatcaa ataatgaaaa tctgtctcaa
     1381 ttaccgagtc ttttttattg acttttttgt atatatgata taattaaata aattacataa
     1441 aggggttaca taaaatgact acagtaaaaa aaaacagagc ggttaccttt caagctaata
     1501 aagaattggt aagcgaagca atgacagtat taaacaaaaa aaatttaacc ttatcatctg
     1561 ctttaagatt attcctacaa aatgtcgttg tcacaaacga ggttgactta ttgacggaag
     1621 aggagttaga gaaagaaaaa cttttcaagc aatttcaagc tgaaatcaac aaaaatattg
     1681 aggacgttcg tcaagggaag ttttatacct ctgaagaagt gagggctgaa cttggactat
     1741 aagaaatatc agattatcta tgctcctgat gttttagaga agctaaaaga aattcgtgat
     1801 tatatttctc aaaactactc ctcaacatca ggtcagcgta aaatggagca aatcattagc
     1861 gacatagaaa aactcgaggt tttcccagaa gttggttttg atgccgatga aaaatatggt
     1921 tcaaaaatca gccattatca cagcactaaa ggttacacct taagtaaaga ttatattgtc
     1981 ctataccata tcgaggggga agaaaatagg gtcgctattg attacttgct tcctactcaa
     2041 agcgactata taaagttatt caaataacac gttaaaagcc ttagcagtct tgctagggct
     2101 tttttggtag attaccaatt aactaggagc aaaatgagtt cgggctgttt aatagatatt
     2161 ccacaaagaa gccaaagact acgaagctag gctaatctac cttaaggctt caggaaactt
     2221 ggaggagttc accaagccaa ctcataaaac ttatagcgag atattcgaga aatggtatca
     2281 agcttatcga gatacggtag agccaaccac ggcttctcgt actcttaatc tgtttagact
     2341 tcacattttg ccagtcatgg gagaattgcc cattaacaaa acctcccctt tagactgcca
     2401 gaacttcatc accgataaag caaagacctt taagaatatc aagcaaatca agtcctacac
     2461 tgaaaaggtc tttgattttg ccatcaagat gaaactgctc aagcataacc ctatggctga
     2521 aatcattatg ccaaagcgga aaaagacacg aatagaaaac tattggacgg ttcaagaact
     2581 tcaggagttt ttagtcataa tacttcagaa agagccttac aagcactacg cactatttcg
     2641 gctactagct tatagcggcc ttaaggaaag gcgagctatg cgctcttaaa tgggctgata
     2701 tagactttca gagtgagaca ctaagcgtca ataagagcct aggacgatta gacggacagg
     2761 caatcaaaaa aagcactaaa aatgatttct cagtaagaaa gattaagcta gacagcgaaa
     2821 ctatttctat tctgcaagaa tggaaagcca tcagccaaaa agaaaagaca gaactagcag
     2881 ttgcacctct ttcaattgag tctgactcta tatttacata ctgtactaga gaagggagta
     2941 ttgaaccact tcatacagac tatattaaca atatcttatc aaaggtcatt aagcaacata
     3001 gtcttaaaaa ataagtcctc acggcttcag gcatacccat gcaaccctga tgattgagat
     3061 tggagttgac ccagtaaata cagccaaaag gttaggacat gctagtagtc aaatgactct
     3121 tgatacatac agtcatgcta ctgcagtcgg agaagacaaa tcaatcaaac aatttgctaa
     3181 ctgtctcaaa aataactaaa taaacgtatt aaaccaagtc aaaagttttc cgaaacggta
     3241 tcaaaagcaa aaaacgacaa aaaaagcacc ttgcaagaga cttgcaaaat gcttgtaatc
     3301 gttgatgtaa agctgttctt aacgttttga gaattgtgaa gctttacgag ctttcttaag
     3361 acctggtttt ttacgttcaa ccatacgtgc gtcacgtgta agaaggccag cgcgtttcaa
     3421 tgaatcgcgg aagtctgggt ctacttgaag aagcgcacga gcgataccat gacggatcgc
     3481 acctgattgt ccaccgtaac caccaccaac aacgttaacg aaaacgtcgt atgaaccttc
     3541 agttgatgta actgcaaaag gttggttgat gatcaaacgt aagtcagcat gtgggatgta
     3601 ttcttctaca tcttttttgt taacagtgat tttaccagta cctggaacca aacgaacgcg
     3661 tgcaacagcg tttttacgac ggccagtacc tgcatattgt gcttgtgcca ttatttaatg
     3721 ctcctttcct cttagataag tcctgagatg tcaagtactt ctggttgttg tgctgcgtga
     3781 gtatgctcac cgccaacgaa gactttcaat ttcatacctt gtgcacgtcc aagagtgtta
     3841 tgtggaagca tgcctttaac tgatttttca atcaagcgaa ctgcgttttt agagcgaagt
     3901 tcaccagcag tgattgattt caaaccacct gggtacattg agtgagtgta gtaaacttta
     3961 tcagttgctt ttttaccagt taatttaact ttttcagcgt tgataacgat aacaaaatca
     4021 cctgtatcag tatgtggtgt gaaagttggt ttgttttttc cgcgaagtac gctagcaact
     4081 actgcagaaa gacgtccaag tggcacatca gttgcgtcaa caacgtacca tttgcgttca
     4141 acttggcctg gtttagccat gaaagttgtt ttgttcatga tttctcctat acgaattcgt
     4201 aatatttgtt tacaaggtga tgggtgttcc gtcccatcga aggtatttgg aaggttccgg
     4261 ggcctttcaa atggggtaaa caataccgcc tactatgata tcaaacttaa gaggctccag
     4321 tcaagtgctt tagctgattt tattggatta ttttttcttt cgatttagga gaatggcaga
     4381 gctgctctgc tggtgttaac gagaatcatc agcttcccaa aaaaggtcat ctaaagtctt
     4441 atcaagcact cgacaaatgg caatgcaaag attaatcgtt gggttgtaat cacctttttc
     4501 aacagccgca atggtttgtc tggagacgcc taccaaatct gctagagctt gttgagacaa
     4561 atctttgcct gcacgcgctg cttttaattt gaggttcttc attcatctgc ctctttagca
     4621 tccagataat gacgatagaa aatactagct cccatactaa ggttacaata tgtcgtttga
     4681 actgccaacc ctccccataa agccaacgta aagaggaagc ttgcagatac cagtccctct
     4741 gtgaatcggc taataaagag tgacagccag ataaccacaa tcataaacca aaacccaata
     4801 gtgtatcctt tgagaagaat ctgtttttgg cgttcatcat atttgggcaa cttctgatcg
     4861 tcccttacct tagaatctag tacttccttg ctgtctagtc tcgtttggag ctttttcgct
     4921 gtctttagcc cccataccaa caatatgaga aagctagtca atacaaaagc tacgcctcca
     4981 actaagtaac gttgaaaact ggtcttagcc agtaatgttg ctactatcac aactagatcc
     5041 atcactgctg ttatgacaat gaaaccccat tgtgacttag cgagattttg tttgctatca
     5101 aacatgattc gtttctcctt taaatcttta tatgacataa tataaattat tttggatatt
     5161 ttgtcaaata tattttacat aattgattga cttaagcagc aaaaaaggat tgaggatttc
     5221 tcttcaatcc ttataaggga ttaacgggaa ttctgcccaa tcacaaaaac tgccagggtg
     5281 ttaggaccaa cgtgagctga gataactggt cctagtggca tcataagaac atcgctaata
     5341 ttctcatgcg ccagcagctc ttctcgtaac ttttcggcac taccctgatc gcttgtataa
     5401 gagacaataa ccgtcgaatc cgcaatatct ttttccactt gagccaccat ttctttgatg
     5461 gctttttggc gtccccgaat ttttgcaatg ggcaccaatt tcccttcctc atcaatccac
     5521 aaaagaggtt tgatactggc taaactgcct agaaaagctg aaccttttga cagacgccca
     5581 ccacgcatca aatgaaaaag atcatctact aagaaatagg tgcgcagtcg agggatcact
     5641 gcttcaacaa tatctttagt ctccagtaaa tttttgccac tatctctggc ttctgctgct
     5701 aaaatggtta gatagccttc tcctcctgca gcagccaagg tatcaacaat ttcaatcacc
     5761 gcatcgggat aatcttctcg aacaagatcg cgtgccatta gagcgctttg ataggtaccc
     5821 gataaaaccg aagaaaaagc aagataaagc agtgctttgt tgttcctagc atgttcacga
     5881 aaaaccttct caaactctcc cacattaatc tgactggttt gaggatgact gccagctttc
     5941 atctttttca aaaggtaatc actactaata cggttaggac cgaccgtttc ataaacctct
     6001 ccatcgcaca aaatcgttaa tcctataagg acaatatcat gatcttctgc ccaggtttga
     6061 ttcaaatcag cggttgaatc tgtcattatt gtaaaggtca ttcttacttc tcctcatcca
     6121 gttttttagt ttgataatca aataattatt gttttatttt atcaaaaaaa cccgcacttg
     6181 gcgagtcgta attctcatta tcgatctaac tgagacataa aatccttcaa ttgaataagc
     6241 tgtccttggt caaaaggccg cagtttaggc gttttgagat caatatctct agacatttta
     6301 tctaaatcag ctttgacagt atgaactctt tgctctaatt ctcttgcagt gacacgaaga
     6361 gctccttgag aaaaaatcgt ccattgttca tttaaatcac tcgtagctac ttctaccata
     6421 tgtatagccg tatttaactc tgctgccatg cgctcaatgt agctatctgc tgtttcgtct
     6481 tcctctgtaa ataccactga gatataatac tggtcatatc gttgccttag ccccggtaca
     6541 tattgggcat caaaaacaca aataatatta atattctcaa aatgggcata atgattaagt
     6601 tttgttaaaa gtgtgttacg tgcctgatca agctgatttg tcttaaacaa ctgacgggtt
     6661 gattgccaaa aggcaatcat attataccca tctactaata atatccgttt tttcataatt
     6721 tagcgcctat ttcggaaaac ttcatacatg agaatagctg ccgctacgct agcatttaag
     6781 ctctgtacgt gaccattcat aggaatcgta atcatttcat caacttgctt tttgatattg
     6841 gtagagatgc ctttgccttc gttaccaatg actaaagcaa gtttaccatt agtgttccag
     6901 caatcagacg gtgttccatt catgtctgtg ccaaaaatcc agaatcctct tgatttcaat
     6961 ttatctagag tttgactaag gttagttact ctcgctatcg gaatatgttc aacagcacct
     7021 gtagacgtct ttgaaaccac tggagtcaca ccaacagaac gatgtttagg aataatcact
     7081 ccacatacat ttgtagcatc agctgtccgt aaaatcgatc ctaaattatg aggatctgtc
     7141 agaccatcta aaataagaat caaaggattt gcttcttgct ctgctatttc gaggatctca
     7201 tcaacatcag tataggcaaa agctgatact ctcagaacaa aaccttgatg cacagctcca
     7261 tcagtcattt gtgacaaggt ttttttaggc gtccatgaaa tagcgacctt tttttgtgtc
     7321 gctaagcttt tgatgttatc cactttcttt cctcttaaat cctcttggat ataaagctta
     7381 tttcctgtat ttgcttgaag gctttctgta acggcatgaa caccatagac gatatcgttt
     7441 gtttcaatag tatctttatc ttccatgagt ttattatacc atgaatgctt caaaataaat
     7501 gcatagactc ttttattttc gtctagatac tttttgaata gctataaata aacctgcagc
     7561 taaactgact actagaccta ataagaccca agggaatcct ctttttgaag taggttttac
     7621 ttcctctttg tccactagca tttttgtctt gttgctaccg tttttttggc tattactagc
     7681 agttggtgta agtttctcat ttttagtaga tcgctttaag gaagttgatc gatgattagt
     7741 agctgatctg aaaggatttg aagtagaatc tatttcttgg ttagctgatt gatttgtttg
     7801 agcttttgtg tctacatcga ccgaattcgt cgttgcttta gaagtatcct tttgttgcat
     7861 attagttggt gtttttctag ctttgttttg atcaaaatga acagttcctg attgaccagt
     7921 tactgccttt tcttgagtat gaacggtgac agttacatga tagtcaccac tcttttgtcc
     7981 agttaaatca aaccaagctg taaaacgacg ctgttgcgct gccatagtat atacccaact
     8041 attaatagta ttatcactag cgtctttaag tgttatcgtt gcctgagttg gttgtctgac
     8101 aggttgatct gtggtgactg tagcatcaat ataaggttct gttccacgca tactggcttc
     8161 aacactgacc tgactaatag ttgattcagc tataacatta tcagtgtgct gatcttgtgc
     8221 atacacagat atggctttgc cagaagtccc cagcaaacca ataagtaaag ctagtgataa
     8281 taagctaact gttttttgca tagttgtcat aataaatatc tcctctttaa attagtctta
     8341 ccctctcatt ataaactatt cgaaaatgat ttcattattt ttatgtcttt tttgttacaa
     8401 atgtatcttt tttatatcat tatgtctttt tgatgtcata aaaagactta atggctctct
     8461 acctttactt cagacctatg aaaataagag tctcctaggg gactcttatg aggatatcaa
     8521 ttgttgcttt tctacatact caatacacca tctgatcaat tcttctaacc gctctttttg
     8581 gcccatcata tcaagataac ccataatggc ttcaaagcct gtcgacatac gataggtaat
     8641 aatatcggca ttcttagctt tagtatggct attggtattg cgaccacgtt tatagatgtc
     8701 ttcttctttt tcggtcaata ggtgcgcttc taacatagcc tgaatcaagt tggcttgtgc
     8761 cttagcagaa acatatctcg ttgctaaacg gtgtagctgg ctaggtttcg ttttaccttg
     8821 aaaaatgaga tgacgacgaa catagtagga gtaaaccgca tccccttcaa aggctagggc
     8881 aataccatta atcaaattca catcaactgg attagtcacg cagccacctc acaccatctt
     8941 tggtatctag cagcttgatg ccttgagctg ctaactgatc tcgaatggca tcagctgtgg
     9001 caaaatcacg attcgcgcgt gcttcttgtc gcttagcaat caaggcttca atgtcaacct
     9061 caagcacctc ttcttcaaag ataatgccaa aaacagctaa cattttctca aaagcgcttt
     9121 taacaggttc tgtataagag ccagaattaa tccacttagc catgtcaaat acgacggtaa
     9181 tcccatttgc tgtattgaaa tcatcgtcca tagcgtcttg gaaagctatt acaaactgtt
     9241 ttagctcttg ctcatctgca gtttctgtca gcggttgttg caaggtgttt ttaagatact
     9301 tcagattgat ttccgcatca tgaatagcct tttcagtgaa gttaattggt tttcggtact
     9361 gttgtgtcgc aagaaagaat cgcaaaacct gaccatcaac ggtttgtaac atgtcatgaa
     9421 cggtcacaaa attacctagt gacttggaca ttttttcgct atcaacagtg acaaacccat
     9481 tgtgcatcca atagttagca aaagtcttgc ctgttttggc ctcagattgg gcaatttcat
     9541 tggtatgatg aggaaattcc aaatcagctc cgccaccatg aatatcaatg gtatcaccaa
     9601 gaatttcagt agccatcaca gaacattcaa tgtgccagcc tggacgacca aacccccagg
     9661 ggctatccca agaaacctca cctgccttag ctgatttcca aagagcaaag tccagtggat
     9721 tttcttttaa agctgtctca gcatctgttc gaccactggc tccaacttca agttctgaca
     9781 gggttttatt agctagctta gcataatgct ctgacttttc cacgcggaaa tacacatctc
     9841 catctgcttc gtaagcaaaa tctttttcaa tgagactttc aacaaatgaa atgatttctg
     9901 ctatataatc cataacacga ggattttgtg tggctggctt aacaccgagt gccttggtat
     9961 cttctataaa agctgcaata aagcaatctg ataattcttt aggagaaaca cctgcttgag
    10021 tagcagcttt gataatttta tcatcgacat cggtaaaatt ggaaatgtaa ttgacctggt
    10081 agccagtata ctcaaaataa cgtcgaatgg tgtcaaaagc gaccgctgat ctagcatttc
    10141 caatatgaat ataattatag accgtcggtc cacaaacgta catattgact gtattttcag
    10201 tcaaaggtac aaacttgcgg agcgaacggg tcatggtatc ataaatttta atcatgtaaa
    10261 ctccttattc aatagcctgg cattagttgt catcttgata tcctaagtct ttttctgaaa
    10321 tgctgttcca ccttttcaac aatttgatat taataaccaa acctattaac caaaataggg
    10381 tcaaaatgaa gtatataatc gctttaacca tgtcttgtct cagtaaaaaa attagactag
    10441 ccaccagtgt aatactaaaa acaaggatag acaaactcat aatgacatac agcccaactc
    10501 ttattttatt catgaggctc ctttattaac gaaaaacctt cacaacctgt gaaggcttca
    10561 gaaagtagac aaacccactt ttatgctcaa aaagcatgaa tagatctact gatttgacat
    10621 caggtttcct atttctgcat gtggggtgag ccaaaacagc tgctttttgt tgtggtaaaa
    10681 gtggcatcaa tattgacagt gatacccata cggatttttg agatcttagg ctcaaaacta
    10741 aggcatggaa ttccaaagga agtcgctgcc gtaagcacca cctgagaaaa agtatcacaa
    10801 tagactgttt cttcagtcta agacttgctt agttgatgct cttttacgcg ttttttgtat
    10861 tttgtaacat tcccatatca ctacactcag caaccaaact gatagggcta gctaagttca
    10921 acattactct actagaaagt cccaaaatat tttattggaa gtcatcagcg cttctttttg
    10981 ttattttccg atcattttga caactgataa gagacctctc gttgtttttg taaactttga
    11041 atttgacgat tatctttttg cccatgcact cgtactatct tagctggcac acctacaact
    11101 gtcacgtctt caggaacatc tgataacaca acagctgctg cccctacttt agcatttgct
    11161 ccgatgtcaa taggtccaat cacttgggca tgtgccgaaa ttaaggcacc ttgtcgaatc
    11221 gttggatgac gcttgccaca atcttttccg gttccaccta gggtcacccc atgataaagc
    11281 atcacacctt tttcaacaat tgctgtctct ccaataacaa gaccagcacc atggtcaatg
    11341 aagactccag gagcgatttg tgctccagga tggatttcaa tttgtgtcca aaatcgccaa
    11401 aattggctat gcattctggc tagtaattta aaatgatgtc gccaaagaaa atgagacaaa
    11461 cgatgagcag ctagggcttt gatgcctgga taagttagga taacctcaag actattacga
    11521 gcagctgggt ctagagcttt aacaatagct atactttctt tccaccaacc catactaact
    11581 cctacgactt atctaattct gaccaatcaa caagaatttg ttggtcatca aaaggattga
    11641 tggcgacacc gaacgtttca tcaagctcgt tttcaccatt tcttggttga taaatatcat
    11701 caatcgtcca agtcagaatc actccttcag ctttcctgaa aaggcctcca aaatcgtctt
    11761 ggttgtacca tttcgcaaag ctttgaagat ttgaaaaggc gggaacatag ctttttcctt
    11821 caggcgttga catggtagga aaaaggcggt cgtaatggtt attatcttta ggataaacaa
    11881 aggcaggaac aaggtaaacc ttttccatcg tatctgccgc cacattgtca tcactcataa
    11941 gcgtatttaa cacagtggtg taatggttca taaattgaat catatcactg cttttgaaaa
    12001 tggtagaatt cccaaaatct ccctttttct taaggttaaa gacaagacct gctgcaccag
    12061 atgtaatgac ttcttctagc actgcaagag ctgaacgctc cagccaatac tgactttgag
    12121 cactcttttg ttcttcttta aagcgagcca tatcttcctt atcggtaaaa acaggggtaa
    12181 ccttgacacc ttcgacttct atcacgtagg gttctttagc agcccagaca ggaaaattat
    12241 ggaaagcgtt gacaagcgca aggctatcca aaaagttatc aggcgcattg ataaaagctc
    12301 ttaagcgaat atctaattca tttgatttag tcatgttttt cttctttttt ctctggtttt
    12361 ggtgggcgtg gaatcaaagc tttcattgag gcatccacac gacccttctc atcaatctta
    12421 atgaccttaa catcaacgtc ttcacccact tcaaggacat ctgacacgtt ggtcgttcta
    12481 gtccaagcaa tttcagaaat gtgaacaagt gcatctgtct tgtcaaagag gttgacaaag
    12541 gcaccaaatt tctcaatacg gacaacctta gcatggtaaa cttcgcctac cttagcttca
    12601 cgtactaggc cagcgatgat ttctttagtg cggttaatgg cagcttggtc acttgagtag
    12661 atagagacat ttccttcgtc atcaatatca attttgacac ccgtttcggc aataatcttg
    12721 tcaatagttt cgccaccttt accgataacc actttgattt tgtctacgtc aatcttgatg
    12781 gtgtcaattt tcggtgcagt tggggctaac tcaggacgtg gttcagcgat agttgcttca
    12841 atcacatcaa ggatttcaaa gcgagctttt ttagcttgtg caagagcttc ttctaggatt
    12901 tgaggcgtaa tccctgcaat cttaatatcc atttgaaggg ctgtaatccc ttcacgagtt
    12961 cctgctactt taaagtccat atctccaaag tggtcttcaa gaccttggat atcggtcaag
    13021 acagtataat ttgtcccatc ggaaatgaga cccatggcaa tccctgccac aggagccttg
    13081 attggtacac caccagccat cagagcaagg gtaccagcac agatagaagc ctgagaagac
    13141 gaaccgttag attctagaac ttcggcaact aaacgaatgg cataaggaaa ctcttctaag
    13201 cttggcaaga cttgttcaag agcgcgttca cctaaggcac cgtgtccaat ttcacgacga
    13261 ccagcagctc cgtaacgacc cgtttcacca actgaatatt gcgggaaatt gtagtgatgc
    13321 aagaagcgtt ttttgtactc tggggccaga ccatcaatga tttgagtttc tcccattggt
    13381 gccaaggtca aaattgatag cgcctgagtt tgaccgcgcg taaaaagacc tgatccatgg
    13441 acctttggca agaagtcaac cacagcatca agcggacgaa tttcgtcaat cttacgaccg
    13501 tcaggacgaa tcttgtcctc tgtaatgagg cggcgcactt cagcatgctc catttgttca
    13561 aggatttctg ccacgtcacg cataatggtt gctaggtttt cgtcttctgc gtaacgttct
    13621 tcgtattcag ccttaaccat ttctttaacg gcttctgtgg ctgcttcacg cgcttttttc
    13681 tcttcaactt gaacagcttt ttgcagttga gcattgtact tagccacaat atcagcttgt
    13741 aagtctgcat caacctgtaa cagttcaacc tcagctttct ctttaccaac aactgctacg
    13801 atttgttctt ggaaggcaat taattcttga atggcttggt gaccttttaa aagagcctca
    13861 agcatgatgt cttctgataa ttctttggca ccagattcaa ccatgttgat ggcttctttg
    13921 ctaccagcaa cggttaattc taagagcgac gcttccattt gttctttatc tgggttgatg
    13981 atgaactcgc catcaatata gccaacttga acacctgcaa tcggtccatt aaatggaata
    14041 tctgagatgg acaaggccag agatgagcca aacatcgctg ccattggagc gctagcattt
    14101 tcatcataag aaaggacagt gttaatcact tgtacttcgt tacggaaacc ttcggcaaac
    14161 atgggacgaa tcgggcggtc aatcaagcga gctgtcaagg tcgcatctgt tgaagggcgt
    14221 ccctcacgtt tcatgaaacc tcctgggaat ttaccagcgg catacatttt ttcttcgtaa
    14281 ttaacttgaa gagggaagaa atcacccgtt gccattttct tagacatgac agctgcggtc
    14341 aggacagttg agtcgccata acgaacaacg gttgccccat tggcttgctt agcgacttga
    14401 ccaacttcaa caacaagggg tttccctgca aatgttgttg taaaggtttg ttttgacata
    14461 tattctcctt ttaattttca gaggggtaaa gaggtacctg ttctctgctt agattcaatt
    14521 gttatacgtt gttttgctac aaaaagcaac ctgccacgct tgtcacacgc tcctctttgt
    14581 atcaaacgac gtatctgcta cattttacca taaaatagca gaaatttcac tgtcagatcg
    14641 caatctcttg aaatgtcctt gaaaaatatt aaacgaaact tatactcttt taacaaagca
    14701 aaaaaagcag gttggtatgc ctgctttcgt ctcctaatat taatgattgt catcctctaa
    14761 aattttaaag ccgaggctgg tactgtcttt cagaaacatt tgcacggttt caagggaata
    14821 gtcttctaaa tcttttcctt gaagaggcaa cttggcaatc aaatcagtgg tacaagtgat
    14881 aatgtccacg cctagctgat cagcttgata gatattataa gtttcacgag gactagccca
    14941 caacaattcg accccttctt tttgaagaca aatgcgtaga gcttcttcca tgattggcat
    15001 tgggtcaacg ccggtatctg caatacgacc agcaaaaaca gacacaatag ctggcactcc
    15061 agcggttaaa tgatcaacca cagcttgagt ctgctcaatt gtaaaaatgg ctgtgacgtt
    15121 taacttaatg cctttggctg acaacttttg aataacggaa caagttgatt cgcctgttga
    15181 agtagtgact ggaattttca catagacatt gtcacctaga ctggcaattt tctcagcttc
    15241 cttttccatg cttgcgagat catccgcaaa gacttcaaag gacactgggt agtctgtaat
    15301 tttggcaagc acttctttgg caaagccaat ataagaactg atgcctgctt ttttcatcaa
    15361 acttggattg gttgtaaagc ctgtgaccaa acctgtttgt aaatccttta acatggtctc
    15421 taagaccgca ccgtctgaaa agactttaac gtttagtttt tttgtcatca tgtcctcctt
    15481 ttagatttct tctgaaacat tattgatggc ctcttgagat tttgacagaa agtttggaag
    15541 caccaataca atagcaagca cagcaaccac caagtaaata ccgatttgac caatcgacgt
    15601 tcctaagcgg cccagtaaaa tccctaaaac accgaaatcc acatctccaa aggtcgtgtt
    15661 tgaaaaacca agtttaccaa gtacaggtag aagcatggct ggcaagaagg cgagtaataa
    15721 gccattagcg aaagcaccaa tcatcgcacc gcgacgacca cctgttgaat ttccaaaaat
    15781 ccctgctgtt gcaccacaga agaaatgagg caccatacct gggataatca ggacaccacc
    15841 tgcaactcct aaaatcaaca tcccaagtaa gccaccaacg aaacttgacg caaaaccgat
    15901 gataacagcg gttggcgcat aagggaagaa taccgcacag tctacagctg gaatagcatt
    15961 tggaattaat ttgttagcaa taccttggaa ggctggaatc aagtcagcca aaatcatgcg
    16021 aacaccggcg tagacaattg ccacaccaac cgcaaaggta agaccactct taatagcaaa
    16081 gataaatggg ttttgacctg ctgctaattc ttctgttact gaagcattcc tcaaaacaga
    16141 agccactgtt gccactaagt agaaaatcac catgataagt cctgttgaaa tggtggtgtt
    16201 gcgtaagaaa ctccattttt cagaaatctg aaggtcctcg gtatctttac tgtctttgcc
    16261 aaccttgcta ccaacccagg cagaaaggta atagcccaaa ctaccaaagt gtcccatggc
    16321 aatttcatct ccgtcagtca ctttcaacgt atactgttga ccaatggctg gcgaaatagc
    16381 tgaccaagct cccaagagga acccacctaa gataatcaaa agacttcctt tgaaaccaac
    16441 agctccaagc acggcggaca agagacaagc cataaagaag ctgtgatgtc ctgttaagaa
    16501 aatgtatttg aagcgggtaa aacgagcaaa agtcaaattg agcaataacc caaccaccaa
    16561 gatggacatg gtttccacac ccagaatctt ttgagcgaca gaagtgacgg cttcattatt
    16621 tggcactacg ccagtgatgc taaaaccgtg ctcaatgagt ttggcaagag ggtctagatt
    16681 ggtcacaata acacccgccc ctgcagcaag cataaggtat cccaaaattg gccccaaggt
    16741 tcctgtcaac accttatgag caggtgtttt caaagccacc aaccctgcaa aggcaatcaa
    16801 gcccattaaa aatgcaggtt cttttaaaat atctcgaata aatgataata atgcttccat
    16861 agtatcttcc tatctccttt aaaggcttat tccttgccac tcatttcctt gagaaccgga
    16921 accaaatctt cttcaatttt tttacgattg gtatagcttt taacgataca aaccttggca
    16981 tcctctggaa aaatatcttt aaattcttta acggtaacaa acaaatctgc accctttcca
    17041 acagccgcat tggaatcaca ggattctgca tccacatcaa tacccaaact agatgcgata
    17101 gcttctactt tcatgcgaag taacaagcta ctaccaatac cgtttccaca aaccgttaca
    17161 attttaatca tgtgattcat ctcctttttc aatcatatgg ctaatcagac tcattatttc
    17221 ctcagtattc ttagcctcaa ttaattgctc aatatgttca tcgtcatcta aaatcaacga
    17281 cagttcctgc aaagccttca aatgtgaact agaatccaca gcagacaaaa caaaaatcaa
    17341 ttgcacttgc ttgtctggat caccttcttg cttcaaatca aagctgacag gttcttttag
    17401 ttgtaagaga gacatgccta actgccgcgt tcctttttca ggcgccgcat gaggaacagc
    17461 caccttaggg gctaaaacaa tataggcccc aagctcattg accgaatcaa tcatgccatc
    17521 gatataagat gtttcaatca tctggtgttc tagtaaaggc tgagcagcca aacgaatcgc
    17581 ttcctgccaa tccgaaacag catcagcctg cctaataaaa tctgctgtta acaagtccaa
    17641 tagtcctaga cctccttttt caatcgttaa atggttacca atcaggtaac gggataaatc
    17701 actaacaagt ccttctttat tagtaatcgt ggtgtgttta tgaataatac tcaacaggtc
    17761 atccaaagcg aattgctctg acaagggaag atgaaagtca tcacagacca tctttttcaa
    17821 catcattttc tcaacaggcc ccatcaaagc ttgcgtcaca taaacaggtt tagcacagtc
    17881 aaaagctact gttgaaaaaa tcaaatcaaa ggaagctgga tccaacaact ttattttgtc
    17941 cagctggtga atcctaataa actgtagctg tggaaaaagt tccttcaagg ttgcctccaa
    18001 catcagagac gaactaatgc cattagggca aacagataag gctaccagct gattgctcgg
    18061 cctcttctta ggggcctgca accatctccc aaaatgaatg gtaaaataag caacctcatc
    18121 ttcattaact gatttcccca attgtttttc tagaggagaa aggctgcgct tgaccaagta
    18181 aaagagagat tcataatctt gtttgatttg ttccttcaaa ggattggtca agttaatgtc
    18241 gtagtaaagt cggtaataag ctggtaaaag gtgactgtat aggttctgac gaagttctgg
    18301 ggtatcggtg atagacaaac ccgtattaac cgccaccgaa ttgatgattt cctccataat
    18361 atcatagata ggctgctgaa agactaagtc taagtcacct tgaatacaac caagtaacct
    18421 agattgaacc aaatattttt cttcggttag actggggaag ttgaccaata actggtccac
    18481 catcgtttca acagctggcg aagctaaagg aaaagtgtcc actgcttcac cccttacatc
    18541 ccgtgaaaat ggtcgatgag cgagaaggca gaaaaaataa atcaaatccc tcaatttctc
    18601 cgaaataaaa gttaagtggt tctccctgct cagttcctcc aacgttgctg ccatcacctt
    18661 tttctggtcg ggcaaaccaa gttcatgcaa taagtaactg aacacccaag gtcctgatgt
    18721 gacttgcaaa agagaactga ccgctgattc taagagacgc cgcaaggcta agggttctcc
    18781 ttcaaaaaaa taaccatcct ggcgattgta agctaaagtt acctgaaaag cttgtacccg
    18841 atcacgcacc aagtttaaat cagcaataac ggaatttctg ctgagccgta acaaactttc
    18901 caagtggtta atggagataa attctttagc catcatgatg taaatgataa tcatgtccag
    18961 acgctcatct tgaaagagat agtcgtctaa ggtaggtagc atgcgattca acaagtcctt
    19021 ttcagtaagg tctggcggat aaaccagctg cctgtccaga gcaataaggg gtaactgata
    19081 gagtgacaac gtgtcattga ttttagcaat atctgccagt atatgacgtt tgcttgtctt
    19141 taatatggat tcaaatgcct ccagtgaatg tcctttatat ttagaaaaga gttgatagtt
    19201 tctgatgagc tcatgagata gcatgtcatc tcctcctata atgtcagtat aaaatgaagc
    19261 gttttcattt tcaagacaaa ttgagtccaa ttgtctctcg tcatttttga ctgcaattgg
    19321 actcaacagg tgatatcatt gtaacaagtt cctcaaaagg tggcaaaaaa taggcaaatc
    19381 tgggaattga ttcccttttt cttcatgata caacaaaaga tacctcatca ctgagatacc
    19441 ttttgttgct aaataaaatc atcaactgct aaaaaaacag tcttagacca ttctagccaa
    19501 atgtgaaaac taacaaacaa catgtcaaaa atgaaaaagc ccactgcata agcagaggac
    19561 tttttaaact tgaaattaac gacgaagtcc aagagattgg atcaactcac ggtaacggtt
    19621 aacatctgta cgacgtaggt atgccaacaa gttacgacgg tgaccaattt ttttcatcaa
    19681 tccacggtaa gtagcgtggt cttttttgtg ttctttgatg tggctgttca agtggttgat
    19741 ttcccatgta agaactgcaa cttgaacttc tactgaacca gtatcgcctt cgtgacgtgc
    19801 atattgagca atgatttcat tttttttctc ttttgagatt gccataatat attctccttt
    19861 ttttgcttaa tccgagtcct aggattggca ctcctaaaac caagaaaaag ttggtttgtc
    19921 tttcgaccac tctattctag cagattcata atattaggtc aaccgttatc ctattattct
    19981 gagctctcac atccaaacca cctcactagg cttaaaaagg ttatctggtc gttgaatgtg
    20041 ctctttttgc tttaaccaaa ccaccataag caacagaact agcaaggcca aatagccaag
    20101 aggctgtttt taaatcagaa taaacctgtg cgattgcttg cctaataaga taaccagcta
    20161 aaagaggtaa gatggctgat cccagcactt cagctgataa gcggtagcct agcatagtca
    20221 ttcatttctt tccaacaaag tgttcactga taatatgaat tgctctagta ttaacaaacc
    20281 caatccctaa tccatgaaca cagcttatta accgatgcga cctgccttta tgacataggt
    20341 caagagttga cacaaaatgt ttgccaacaa aacattacct ataccgtcaa agagcgcttt
    20401 gcttcctata cccttgaagc gcaagccaac caagaggttc atctaaacct tacattatta
    20461 gcaaaccgtt ttggaacaag cgatcgccat cttaagcacg tacttaagca gcccattttt
    20521 cagaggatta tcgaacgcat ctgcttcaaa acctgtcgta ttgccgattt agacgctcta
    20581 actcaattaa gtgttaataa ctagacctct gaggtatgca gcccattaac ttataatagc
    20641 aatcactcta accactacct gacttttctt taaaaaagaa atgatttgtg atatagtaaa
    20701 tccagttaaa aaaaaagtaa aggagtagac atgtctgcac aagataaatt aattaaacct
    20761 agtcatctga ttaccatgga tgacattatt cgtgaaggga atccaacttt aagagctgtc
    20821 gccaaagaag tgagcttacc cttgtgtgat gaggatatcc tattaggaga aaagatgatg
    20881 caatttttga agcattctca agatcctgta atggcggaaa aacttggtct tagggccggt
    20941 gttggtctag cagctcctca gattgatgtg tcaaaacgta tcatcgctgt gctcgttcct
    21001 aatcttcctg ataaagaagg aaatcctcct aaagaagcct atagctggca agaagtcctg
    21061 tataatccta aaatcgtttc tcattctgtt caagatgcag ccctttctga tggcgaaggt
    21121 tgtttatctg tcgatcgtgt tgttgaaggg tatgtggttc gccatgctcg tgttacagta
    21181 gactactatg ataaagaagg acaacagcat cgcatcaaat taaagggata caacgctatt
    21241 gtggttcaac atgaaattga ccatatcaat ggtgttttgt tctacgatcg gatcaatgct
    21301 aaaaacccct ttgaaaccaa agaagagctc ttgattttag actaacaaaa aaaggctaaa
    21361 gggccttttt tgttagtcta aatagccgct aaaagagcct ctgcctgaga tagcaagttt
    21421 gcttttatct cttttgaaat ctcaagcctt cctgttcccc aggcatcagg attcacagtt
    21481 gcttttgtaa actctcctgc tactgaagtt cggataaacg gcaatagtgc tttaaactga
    21541 tcaaatactt gatcatgccc gccatttgca actgaagaga ccgtaaccac cttaccgcca
    21601 atagcagatg ggcccgtcgg atcagacaaa tcaagagcac gagacaacca gtctagcagg
    21661 tttttaacag aacctggaat agagaagttg taaactggtg taaaaatcca gatagcatcc
    21721 gctgactgaa cagcttgacg agcgtcaaca actggtaaag gtgcattagc ttcgatatct
    21781 tgattcaaaa caggaacgtc tttccaattt aagtaagata caactgcttg atgttccaga
    21841 gctttttgtg cttgagccgc taattgatgg ttaaaagacc cttcacgaag cgagccaaca
    21901 ataaataaaa tatgtttcat ctctttgtcc tctttttttt aaagatcgtt ttaaagcttt
    21961 ctaaaagtgg tatttcattg atacttcttt ttgaaattta tcactatgta gtaattatat
    22021 cttctctaag aagagctgta aagagatttg tttataattg tttgaacttt ttgagaagtt
    22081 caatcagaca aagttgttct ttttcagtca aaacagcaaa ggcttcttcc acccgtgcga
    22141 cgtgtttagg caaaacagct tcaatcaagc gagtaccctt atctgttaag gtaaccacat
    22201 aggctctttt atcagttttg tctttgcact tgctaatcca gccattgcgt tccatattat
    22261 ttagtacaac agtcatattc cccgaagtcg ctaacaaaga atcaatcaag tgattgattc
    22321 gcatacagcc tttagtatat agtacttcaa gcacactgaa ctgcgtggct gttaaatctg
    22381 cctttttaaa aatatcagcc ccaaaagcat ctagcgttcg ctgagcttta cgaaacacca
    22441 ccatggcttt caaagcggtg ttcttatcta aatgactcat ttgatttcct cgctttatac
    22501 ggtatgtcac agtttagtat cagagagatt agaaagtgat cttgtccctc ttcttttatg
    22561 actttgcata ttactaccat catgataatc tagaaaaagt catcaaaaag acttaattga
    22621 ttatcttctg gcatatttcc taaaatactc atctcatcca ttttctcaac gagcgttgac
    22681 gatgccccgc ctcgtttacg caattccatt ttagagagga attcgccttc ttgacgagct
    22741 ttaacgattt gcttggccac gttttcaccc agaccttcta acgctataaa tggagggata
    22801 agggtatctc ctttgatttg gaattctata gcatcacttt tgtaaaggtc taatttgcca
    22861 aacttaaagc cgcgttctaa catttcgttg acaatctcca aggttgtaaa gaggtcattt
    22921 tccacattgg tagcttcatt atttttacgt tttatagtaa tatcttccat tcttgcttta
    22981 acagcatcta aaccaccact catggttttt aattcaaaag ccttcgcacg aatagagaaa
    23041 taagcacaat aatacataat ggggtggtgc accttgaaat aagccacccg aagggccatc
    23101 aaaacataag ctgccgcatg ggctttaggg aacatgtact tgatttttcc acacgattca
    23161 atgtaccagt cgggcacatt gttttctcgc atggcatcaa tatagccatt acgttcttcc
    23221 tcagaaattt tcagccataa tcccttacgc acacgctcca taatggtaaa ggccattttc
    23281 ggttctaagc ctgcgtgcat gaggtaaacc atgatgtcgt cacgacaacc gataacggtt
    23341 tttagggtcg caatgccttc tttaatcaaa tcttgcgcat taccaagcca aacatcggtt
    23401 ccatgagata gtccagacaa ctgcaaaagc tccgcaaaag tggtcggatg cgtttcgtta
    23461 accatgccgc gaacaaagtt ggtcccaaat tctggaatgc ctaacatacc agtcggtgtc
    23521 ccaatttgtt ccggggtaac gcccaaaatc tctgtcccag aaaagagagc cataacacct
    23581 ggatcatcag cagggatagt aataggatca atgcccgata aatcctgaag tttacgaatc
    23641 atggtcggat catcatgccc taggatatca agtttcaaga cgttttcatc aatatcatgg
    23701 aagttaaagt gagttgtctg ccaagaagcc gttacatcat cggctggata ttgcacgggg
    23761 gtaaaatcat aaacatccat gtaattagga ataacaacaa tccccccagg gtgctgccca
    23821 gtcgttcgtt tcacaccagc agcacctgct gctagacgat ccacctcagc atcccgatag
    23881 aacttgccat agtcgcgttc atagcctttg acaaatccat aagctgtttt ttctgctacg
    23941 gtaccaactg ttccagcacg aaaggcgtat tcgtcaccaa aaatatctcg gacatctaaa
    24001 tgggcactgg gctgatcatc accagagaag ttcaaatcaa tatcgggcac cttatcccca
    24061 tcaaacccaa gaaaggtctc aaagggaatg tcttgcccat ctttttgata aggggtgcca
    24121 cattttggac aaggcttatt aggcaaatca tagccagatc caactgaccc atctgtgata
    24181 aattcagaat gttggcagga cgggcaaacg tagtgaggcg gcataggatt aacctcggta
    24241 atcccaatca tggttgccac aaaactagac cctacagatc ccctagaacc aactaggtag
    24301 cctcgctcat ttgaccggtt aacaagcatt tgggaagcga gataaatcac agcaaaaccg
    24361 ttccccaaga tagaggttag ctctttttca atgcgtaaat caataatatc tgggagagga
    24421 ttaccataaa tttcaaaggc tttttgatag gttaattcgg caaccgtctc ttcggccttg
    24481 tcaatatacg gggtgtaaag atcgccctta accacttcca cttcctcaat acggtccgca
    24541 aaatcctgag tattttgcac aaccacttga taagcgaggt cttttccaag aaaggcaaac
    24601 tcatccagca tttcattggt tgttctaaag tgcgctttag gtagaggagc aggttgggcc
    24661 ccttccccac ggccgattgt tctattaatc atggcaccct gaccgagact acgcacaata
    24721 atttcacggt aaatctcttc ttcaggctct agataatgca cattcccagt ggcaagcaca
    24781 ggtttcttag ctcgtttccc tacttcaatg aggtcacgaa tcacctgctc aatacctgct
    24841 tgatctttga ttaattcacg gacaaccaat ggctggtaaa tggctggtgg catgatttcg
    24901 ataaaatcat aatacttagc caaatcaacc gccgcatcaa ttcctttagt cagaacggca
    24961 tcaaaaacct cgccgtcaga acaagctgtt cctagtaaca acccctctct gtgagcatct
    25021 aagacggttc ttggaatacg cggcacccct tcaaaatatt tgatattgga aaggctgacc
    25081 agcttaaaca tatttttaag accaacctga ttttgcacat agatagtcgc atgcttaatc
    25141 cgcgcttttt tgtaagaatc ctcagccacc aaatctgtat tgagttgcaa aagatttttg
    25201 atgccatgct tttctctggc atctcttaga aaaataaaca aaagacgtcc tgtggcttcc
    25261 gcgtcgtaat tggccatatg atggtggtct agactcactt ggaaacgctt ggtgagcggt
    25321 cccaaaccgt gacgtttgta ttcaggatac aaatttcttg cgaattctaa ggtatcaatc
    25381 acaggctgtg tgattttggg caagtcatgg cgttcataat tggcgttcat aaagcccacg
    25441 tcaaaactgg cgttgtgggc aaccaagata ctatctttgc aaaagtcctg aaaagctttt
    25501 aggacggtaa ctaatggctt ggcgccctgc aaatgcttgt cggtaatccc tgtcaattcg
    25561 gtggtaaagg cagaaagagg atgcccagga tcaatgaatt catcaaactg ctctacaata
    25621 tttcctttaa acattttgga agccgcaatc tgaatcaggt cattattcat agcagataga
    25681 cctgtggttt ccacgtcaaa gaccacatag gtagcttcgt gcaaatccat atcaacagga
    25741 tcataagaaa taggcacctt gtcctcaaca atattggctt ctagaccaaa aatagcctta
    25801 attccagctt tgcgagccct atggtagcca tgaggaaaac tttgcacatt agcatggtcg
    25861 gtgatagcaa ccgccttgtg tccccacttg gctgccgtat caatcaagct ttctactgtc
    25921 ggtaaggcat ccatggtaga catattggtg tgggcatgaa gttcgacccg cttttgccct
    25981 tctggcatca ggtctttgcg gtcatgatgg acaatttctt tgacctgctg aacattcatg
    26041 gtaagactct tcgtaaaagg attggtctca atattacctt gtacccgtaa ccaagccccc
    26101 ttagcaatca tgtcaaattt acggagctcc tcatcgtctt tggcccattt ttggagagca
    26161 aacgaggagg tataatctgt cattttaaag ttgatgatat ggcgacctgt cctagtggtc
    26221 ttgcgctcca catcaaaaac catcccctca aagacaattc ggttttcttc ggtctcaatc
    26281 tcaatcattg gtgtgatggt cgctttttca aatcctgcct gacgcttagc tgctcgttcc
    26341 ttgtagtcaa acttaggagc aggtgtggct tcctcaactg gtggcatcat ggcttcaagg
    26401 gatttttggg cttccaaatt atcctgcaca gcctttttca caagagcctg acgactggaa
    26461 acaaaatcct tggtcaaatg ctcagtcatc tcctgatctg acaccatatc tatggtcaag
    26521 gtgccaaagc caaaggcttc taattgcttg accaaattag gcagatgatt gtttctaaaa
    26581 tgatcgttat tcacaaaacc tggcgctgca ataatgagtt tgtcatcctc ataagtcact
    26641 ttgagctttg agaaagaaga tttaaaacta gcactattac acggtgcatg ctcaaaagct
    26701 tcttggtaat aagcttgaag cagatcatct gaataatcca cctgagcagc ttggatatca
    26761 aaggttacct taatgtcagc cgcctcaaaa gttcttatca aacgatcatg caattcacga
    26821 taagttgcaa tcggtaaaac cgccgcaaag gcaaaatgaa attcccacaa gcgtgacacc
    26881 gaatgcacct ttacctcgat aatatcagca gatgaaaagg cacttgaacg tctcatgtca
    26941 agtggcattt ctatctggtc catcaattta gcgaataaat ctgacataat tttctcctga
    27001 ttttactagg ctttatttta tcacaattca agatgatttt cgagttttag atggaaagaa
    27061 aagcagggct ttcttctgac gttctttggc taatttattc caagtgatag ctcagctgag
    27121 gtcatttttt tgtcaatctt ttatcgccaa gcgcttttgg taaagcaaaa gccactctgt
    27181 atcagacaag ttcacagcaa aaagaggcca ctctaaacag gcagcctctt catttctttt
    27241 ttagtgttct tttgttaaaa tttcaagggt ttcgatgagg ttttctgcat taacttcaat
    27301 gctggcacct gttgccttga ttttaatttc cacgatacct tcagcggctt ttttaccaac
    27361 agtgacacga attggcaaac caatcaaatc gctatcagag aatttcgaac cgacgcgttc
    27421 attacggtca tctgtcaaga catcataccc tttagccgtc aagtcagctt ctaacttcgc
    27481 cgtcaagtct tgggccactt gatctttgac attaactgta atcaaatgca cgtcaaatgg
    27541 ggctaattct ttagggaagt taacacccca agcataacgg taatcgcctt ttggtgtctt
    27601 gttcacaaag agacgggcat gctgttcaat aacagcagac aaaatgcggc taaccccgat
    27661 accataacaa cccatcacaa ttggaacggc tctgccattt tcatcaagaa tcgttgctcc
    27721 catgctgtct gaataacgag tgcccaactt gaagatatga ccgacttcga taccacgcgc
    27781 aaactggaga acaccatgac catccggaga catttcccct tctttgactt cacgaatatc
    27841 tacatattca gcctggaagt cacgtcctgg attgactcct gtcacatgga aaccatcctt
    27901 gttagctcct gcaacggcat tggtaaggtt ttgcactttg cggtcagcca caatgcgact
    27961 accttgtgcc aagttgacag gcccaagtga gccaaaacct gcaccaaaga aagcacgggc
    28021 ttcttcttca ctagctggct ctaaaaaatc agcagctaga tagtttttta atttaacggt
    28081 attgatatgg tcatttccaa ctagcaaagc aacaacaggt tcattatctg ccacaaaaag
    28141 caaggttttg atggtttgtg tttcatctac tgaaagaaaa gcagccactt catcaatcgt
    28201 tttgcaatgt ggtgtctcaa cttctgccaa ggcatcttca gcagctactt ttgaggacgg
    28261 tttgtattcg ttagttgcca tctcaaggtt ggcagcgtag cttgattctg ttgaataagc
    28321 aatagtatct tcacctgaaa tcatccaagc agccaattct gccttaatct cttctaagac
    28381 ctcttttgga atatcatcca ttgacgcaat agacttgtca agaaccaccc agcgatcaag
    28441 gtccgtacga gctggtgtaa tggccataaa ttcttgggaa tctttccccc ccatggcacc
    28501 gccatctcca ataatccctt tgaaatctag accagctctg gtaaaaatag cttcgtaagc
    28561 ttgacgataa tcttcgtagg tcacatctaa atcttcatag ttgtgatgga aactatagcc
    28621 gtctttcatg ataaactcac gggtacgaag caagccatta cgcggacgtt tttcatcacg
    28681 atacttagac tgaatttgat aaaggtttaa gggcaattgc ttgtaagatt tgaccgcatc
    28741 acgcaccaaa gtcgtaaagg tttcttcgtg ggtcggaccc aagataaagt ctgagttatc
    28801 acggttttta agcttataga ggtcctctcc ataggtctca taacggcctg attcacgcca
    28861 gagatcagct gtcaaaagag ctggtgccaa catttcaaca gcaccgatct tttcaaactc
    28921 ttcacgcatg atggtcttga atttctcaat ggtacgattt gccagtggta aataagcata
    28981 gataccagca gaaacttggc gcacataacc ggcacgcacc ataagcgcgt ggctgataac
    29041 ctgggcatca cttggcattt cgcgcaaggt tgggataagc agtttacttt gtttcataga
    29101 ttattccgtt ttaatacatc cccttatcct caaacccttc tggaaacatc aagtaagagt
    29161 gaagcgggat tattcaataa tttgaatgtg tattcctttc attttgagta agacctcaac
    29221 ccttgcaaat cttagggttg aggtcttttt cgatctttaa catgataaag tcagcttaag
    29281 atgacctatc ttgattagaa aaagacgcgc atgatatcat tccatgtcac agcaatcatc
    29341 aatacgacca tgatagcaac cccagctagg gtgatatagg cctctgtttc ttgcttgata
    29401 ggcttgcgac gaatggcttc aatgatattc atcaagattt ttcccccatc aagtgcagga
    29461 atcggaatca ggttaaagat ccctaaattg atcgaaagca ttgccataag agataagact
    29521 gactctaagc cgttttgagc agcctgattg gacatgtcat acatggcaac aggtccacct
    29581 aatttattga gactaaagcc aatgatcaat cctttcaaag cattcaaaat agtaaacgcc
    29641 ccactccaag ctaattctag accgcctaag agcttatcct taaatcctgt tttcagactc
    29701 gccttaactc ctatcgtgta tgtctttgca tgcttttgtg gtttcacagc caccgttttt
    29761 aagcgctgat gtgacttgta ggtgacctta atggtctgtg acgggcctag atcccgtgtt
    29821 gcaaggtcta cagcctcagt aagatcattc cagctagtca ccttgtaacc attaattgcg
    29881 acaatttggt cattatctcg aagaccagcc ttagctgctg ctccattttc ttgaacacgg
    29941 acatgattgc tactaaaatc tggcatccca ccttgtaaaa agaccaagag gataaaaacc
    30001 acaatcccta aaataaaatt attcatggga cctgcaaaat tggtgattaa acgtccgcca
    30061 atactagcat tttgatattg aacatcaagc ggagcaattc ttatctcagt accgtcttct
    30121 tcaacaatag tagcatcgtg agcgacctta tatgtcttgg tttcttctaa aaccaagcca
    30181 gtaatactca gctgatcttc taagtcatag cctgtaacat gcattgggag actcgttggg
    30241 tctaacttac tttgagacaa gttgatgcgc ttaacaaaac cttgctcatt aagggttaaa
    30301 cttgctggag tacctgtttt gatctcagtt ttgtcatcac cccaaccagc cattcgcaca
    30361 taaccgccca agggtaacat cctcaaggta taaagagttc ctccttggtc aacatgagaa
    30421 aaaattttag ggcccattcc aatggcaaat tctcttacta gaatgcctga ttttttagca
    30481 aaatagaaat gtccaaattc atggacaatc actaaaatac caaaaataat aataaaggtt
    30541 attattccta acatagattt cctttcagtc tattgctttt ttgagttctg tcgcgttgtt
    30601 gcgactccaa acattacctc ttaagattag aataaaccaa agagatgcat gatgggaaaa
    30661 acaaaaatca tggaatcaaa gcggtctaaa ataccaccat gtcccgggat aagcttacct
    30721 gaatctttga caccaaagtg acgtttgaga gcactttcaa ccaaatcacc aaattgggca
    30781 aaaatggaaa aaagagcgac caataccaac atggtcaaaa agtgatgtgg cgcataaaca
    30841 gacctatcaa tcaccataaa gataaaagac acaaggactg cacaagcgat ccctcctaaa
    30901 ctaccttcaa tggttttgtt tggagaaact gtaggcaata gtttgcgtct gccaaactga
    30961 cgcccaatca gatatgctcc aatatcagtg gcccaaacga taaataaggc caaaaagacc
    31021 ttgtcaattc ctgacaggcg agcattgatc aaatgttgaa aaccaatccc aacataaaaa
    31081 ctagttgcaa tcggaaaggc tgcatcatca aatgaataag ctctgctatt taaaacagta
    31141 cctgccaaaa tgaaaaaaac cattaagctg taaaaagcaa cattcgcatc aattggtaaa
    31201 aaagtgaggt aatggtccat cggaactgcc aatacaaagg cggctaacat ggcaaaaaca
    31261 ccttcaaaag aaaagacttc taaccttttc attttcagta attctgatac cccaatcatg
    31321 gccaaaacgc ctacaaataa ttggaatggt aggtttccta ttatgagaaa aggtaggaaa
    31381 atagctactg ctactccccc ccaaacaaca cgttctttca taactatctc cttttagacc
    31441 ttgccaaaac gacgttggcg gtggttataa tccgcaatgg cctttagtag ctctgccttt
    31501 ttaaaatcag gccacaagac tggtgtgaaa taaaattcac tgtaagctga ttgccaaggc
    31561 aagaaattgc tcaaacgcag ctcaccactg gtgcgaataa tcaaatcggg gtctcggtag
    31621 agatatggca gatggtctgt catcaagtaa ttagcaatca aatcctccgt aatatctcct
    31681 ggatttaatt tggcatctaa gacatcttga gcaataaagc gaacagcact ggtaatctca
    31741 gcacgtcctc cataattgag agcaaaattc aaaattaaac ccgtattacg ttttgttttg
    31801 tcgatggctg catttagcgc tgccaaggta tcttcaggta aacgactagt ctctccaatc
    31861 atttgaatct ttacattgtt ctcatgaagc acaggcacat atttgtcaaa gaaggttact
    31921 ggtaaattca tgataaaaga cacctcatct tgaggtctgg accagttttc tgtcgaaaag
    31981 gcatagactg tcagaacttt aacccccaac tcagatgcag taatcgttac ttcttgtaaa
    32041 gcatccatcc cagctttatg cccaaacact ctaggtttta gtcttttttt ggcccaacgt
    32101 ccattaccat ccatgataat tccaatatgc tttgggatac tccccaaaac tttttttgtt
    32161 gatttcgctt ttaatccaaa catgtttttt ccttattgat aagcatactt atctcatatt
    32221 ttatcatatt tcacgccttt tggcttgttt tttatcaatt agatcttatc ttcacaaaat
    32281 gttcatctat cctctgatag atgtgtcagc ttttcatgaa cctgttagca tcagaataac
    32341 tgtttcaatc cagggatcaa aacaaaaaag tttggcatat ttcatccaaa ctttccccgc
    32401 cttaatggct ttcaatggca ctgtcagtgt cagtgctatc tactgtctca acagcaggct
    32461 cattaacaac actggtttct tctgtttcaa ccagtgtcgt ttctgtggta gcttttgtta
    32521 caatacgctt aatagctgat agctcaaatg tcaaaaagac gccatccaca tctaacacaa
    32581 tctttttagc tgttgtatcg acttcgtcaa caatcgcaaa catgccacca atcgttacga
    32641 cttcgtctcc tttttcaata gcgtttaact gattttggcg ttcttgcgct tgtttttttt
    32701 gttgacgttg cataaaccaa atcaatccaa gcatcactac aaacattaaa attgttggaa
    32761 atcccataat gtctcctttt tcttctataa aagtcatgtc ttactatatc aaaaatcaag
    32821 cggtatagca agactagacg ttacctgatt tcttctttaa acgagacgct aagcgcagtg
    32881 ctttactcac gagtcaaaaa agctaagatg tcatctggtg tcaaacttga atcacataca
    32941 gccctttggt gctgatcgta actaactagc aaagctggaa ctgtcactaa ttggtagttc
    33001 tcacgaaagg cagcaagctc agcagcatca gcagcgtttt cgctatccac aaaatagact
    33061 tctttttgat tatctgttgc cacttgtgcc aacttaggag caaagcggcg acaataagga
    33121 cagctcgaac gtcctaaaaa gacaatcatg tctttgccac tagcaatagc tgatgtgact
    33181 tctgcaatac tcgacggtat aaaattcgca acgatttctt caaatgtcat ggatgtctcc
    33241 ttctcttttg gtctcatgat acactaaagt agcaattttc tcaatcacaa ggtcttttca
    33301 cagctcagag cttttatagc cgtcaaaaag aatccgcaaa cggcaatctt tttgtctcta
    33361 ttaacaccac cctaatcttt ttggcgcttc atcaacaggc taatagccaa taaaagcaga
    33421 gctactccag ctgctaataa gccttttgag gaggcttcac ccgtttttgg gagcttttgg
    33481 tttgttttag ctctttttgg cctagtcatt aatgatttgt ctaggtcttt tgagccatct
    33541 ggtgttttgg tttgatgatt gcctgtctga ctcttttgct gagagggatt agccgttttt
    33601 gaggatactt tgaggaccag ggcagttagg ccatcaatag tcaagccttc tttggtaaac
    33661 tggactccct taggcttagc aatggcagta acaccagctt gctcagcatc agcaagcact
    33721 tgggctccta gcaaatagcg gtaggcttga ggtaaaacta ccttgcgggt cttgttgtct
    33781 gcattgacaa agacagcata gcgatctcca tttgatgcca ccgtttggta acccatgatg
    33841 aggtcctctt gttgaatacc atcttgtcct gcttgggtga tcaaggtcac atcccgatct
    33901 acctcagctt tggttgcttt tgtaaaggca tctgttgagc gacgcaacgc aattagtccc
    33961 tgtgtatagg cttttgtttg gttgctaatc gggtgagcta tggaatctgt tgccttggcc
    34021 cagtcaaaat gattgaccgc atcagacgaa tcataagaat cgtggatgaa gtaagggtat
    34081 ttcgctacag catcaatcag agtcgcctta tttggcacct tatcatcaga cacctttgtc
    34141 ttgtagtcgg gatttagaag ctgcttggtt cgtccatatt cctgaccaga atggataaag
    34201 gcagtccctt gagcagttaa aatcatggta tttcctagac gaatacgctt gtgaatctct
    34261 tcttcagcca ctttaggatc cttattgatg gatttggcaa tgacatcatg taaggtcagg
    34321 ttgtcatggg ctgcaatata ctgcactaca tctcctgggg catctgcttc aaagttacta
    34381 ggctgtgctt tgatcgtttt gaacagtcct tctagatttt ttgcgccacc agtaataaag
    34441 gctgctgtgc cttcatttgg gaaacctgac ttgagggtat ttctgatatc atcagagaaa
    34501 acaccgaccg tattggttgc tttcatccaa tcttgatctg ccgcaatttc ttttttcccc
    34561 tcatcacctt ggtaggtacg ccagccttcg ccaatcataa tggtatttgg attaatggct
    34621 ttggctgcct taaaggcttg ctcaatagct gccgcatcat ggtcacccat catgtcgaaa
    34681 cgaaaaccat ctaccttgaa ttcacgagtc agataagtaa tcgaatccac caagatacga
    34741 cgactcatgg catgtgtcgt tcctagacga cctccgccaa aactctctct agctgtacca
    34801 tcagcattca taaaatgata gtagttgggt tccaaatctt caaagagata ggttctagcc
    34861 gtgtggttat aaaccacatc aaaaataaca cccataccac gtttgtgaat ctcattgaca
    34921 aggtttttaa gctctgcgat acgtaaagca gggtcattag gatttgccga atacatgcca
    34981 gaaagggcaa agtagtgttg tgggtcataa ccccagttgt aattattgtc tgaagacgtg
    35041 taggctgttg agcggctctt gtccagctca ttggcataaa aataactcaa aaccggtagc
    35101 aattgaacgt gggtaacccc caagtctttg agatagtcta gctgttcaac aaaagctgaa
    35161 aaagtcccat aagggtgtgt taacttacct tctagagcct tatctgacgt aaaatctcgc
    35221 acatgtgctt catagataat agcgtcttca cgctttttaa agttgttaat tttggcaaaa
    35281 tcaaggcctg ttggtcctag tttgcttgga tcaataaagg cagcttttgc tgttttgatg
    35341 tcatcagtag cagtcgcatc attccaggca gcgagagatt tggcgtaagg atccaaaacc
    35401 atgacttttt cctgaccgcg cgtgatttca taaagataat aatagcctgt gtaatcacta
    35461 atgcccttga cgctgtcaga agttagatga gctctccaaa cacccttgtc cgacttggtc
    35521 aaatcagctt gaccaagaac ctttgtctga tcttgtttat cgtaaacgac aaccttaaca
    35581 ctgtcagcac ttggagacca tagcgctaaa tcaacagaac catccttagc tagggtagct
    35641 ccaagttcac catcgtaagc atagagttta tcttttaatt gccaggattg gcgagcgact
    35701 tggctaactt ctccaaatgt aacagtgtag actgcacctt gcgccttaaa atcacccttt
    35761 aatgtcatta cagacgtatc cttgtcaagt gtcaattcat caattgcaac agttttccct
    35821 gctttgtcag tgatcttgat gttttgtttc accgcatctt catcaagccc atctaaggtc
    35881 gtaaaaatgg ctttaatctc atttggcgtg gtttgttcag ctcctttgag actaacctga
    35941 tcaatataat aaggattgtt gtagactttt gggtcagtgt ctttgacaaa aacctgagta
    36001 tgattgtcta actctttaaa cagataatct tttggttgca ctttaatagc atctcctgtc
    36061 ttacttttat caaggattaa aaatccgatt tcgttagctc cttcttttaa ggggacatca
    36121 acataagctc cataaggccc tttatgtgac aagtcaagtc catttggcca gtcggtcgtt
    36181 ggggttttga catctttaaa ggtccagaca gctaagttat cgtagtgtcc cgattgattg
    36241 tggtagttga ttcgaaggta accttttttc aaaggtcgat aagcgtgtgc atggtaattc
    36301 tcgtctatcc aaacttcatt catttttggc gtgagaagcg agatgtgctg gtcctttgaa
    36361 agatgctctc cagctttatt attaatgaga taagacactt gctggcggtg tttagctgct
    36421 agtggcacat ctagataata gccatagtca tcttttttcg cctttgtcat ggtgatagca
    36481 ccatttggcc aatcctttga aggttgatcc acatctcccc acacccaaag tcccaagctt
    36541 cctagcgact caccagctgg caatgtttta aaatgcatac gcaaatgatg ctcagcaata
    36601 ctagcaggat tagctgacga gctgtctaaa actggtttag cctccttact agacgattga
    36661 aaaccatcac cagatttagc attgccatct ccttgttgag cttcatcgcg ggctagtcca
    36721 acaatggttt tagcagcaac ttgatgtgtt aagatactag gggtgcaccc taacatgaca
    36781 gtcccagttg cagcgattac aaaacctatc ccccactttt ttaacagata ctgatagcgc
    36841 tttgatcctt ggttgacttt ctttttcata agacctccat ttagtataac attcaaacaa
    36901 gactatctta tcacattgcg caagcgcttg caagacatgc cgcacttcaa cttagaacac
    36961 agctcaaaca atgactatca ccccttaata ggatctcaaa aatcaaacag ccaaaacgag
    37021 tttccgctcg cttggctgtt gggtaaactc aaatcacata caactgctag gccttcagtt
    37081 tgagacaaaa agcatcccaa ggttgtaggt gcttggtctc taacactttg tccacatctg
    37141 tattggtgat gaccacttct gcacctgcta aatctttggc aaagacttgc tcttgatcag
    37201 acacattgac aacaatcaca tatgtctctt ctccaagctg acgctgataa gcaaacactt
    37261 tatcagctgt tggcaagagg tgataatccg cttcaactag ccagtcttgc tccttacgca
    37321 aggcgattag ttgttggtag gtgtaaaaaa tagagtctgg atcagcaagg gcatcggcca
    37381 cattgatgtc ttggtagttg gggttgaccg gcaaccaagt ctcttgggct ttagaaaagc
    37441 cagcatgtgt gtccttagac cactgcattg gcgttctagc attgtctcgt cctaccttac
    37501 ggatgctatc catcacacgc gcagctgaca ctccatttcc catagcttcc ttagcatagt
    37561 taagggactc aatatcatta acctcggtta agtctttaaa aggataattg gtcatgccaa
    37621 tctcttctcc ttggtaaatg taaggggttc ctcgcatcaa atgcagtaaa atagctaaag
    37681 ccttggctga tttttctcgg taagtgctgt cattgcccca aatggataag acccgtggca
    37741 agtcatggtt attccaaaaa agcgaattcc agccttctcc caactttaat tctgtctgcc
    37801 acttgctaaa aatagctttc agagcaggca cgtctaattc tttggcataa tcccacttgg
    37861 gagcgtcagg tttatgttga agacccacat gctcaaattg gaagaccatg gataactctt
    37921 tgttttcagg gcgagaatac tggcgagcaa tttcaggcgt tgctccccaa gtctcaccga
    37981 ctgtcatcac gtcatggttg ccaaaggttg cttgattcat ttctttgagg taatcgtgta
    38041 agcgcggccc gttacccgtg atctccaagt caggaacctt cccaatcagg tcaatcacat
    38101 ccatacgaaa accaccgatg cctttggcaa tccagaaatt catcatatcg taaattttat
    38161 gacgcaaagc ttcattctcc cagttgagat ctggctgacg tttgctaaaa aggtgcaaat
    38221 aatattgacc tgatgcatta tcatattccc aagctgagcc actaaaaata gaagtcaaat
    38281 cgttaggttc atctcgccaa atataaaaat cacgctcagg actattaggg ttttccctag
    38341 cttcaacaaa ccaagcatgc tcatcagacg tatggttgac caccaagtcc atgataatct
    38401 taatgcctcg ctcattagct gctgctaaga gctcatccat atctgccatg tctccaaaaa
    38461 catcagcaat cgcttcatag tcagagatgt cataaccatt gtcatccatt ggactttggt
    38521 aaacaggaga taaccaaatg gctgtaatgc caagtttttg caagtagtcc agttgactgg
    38581 taatgccttt taaatcacca ataccattgc cattggtgtc tttaaaggat ctcgggtaaa
    38641 tctgataaat cgtagcttta tgccaccagt gtttcttcat tattcttctc ctattaatca
    38701 ttcattcatc ttttatttta acgttttttc tctttttgtg caatcgtttg cgcaaatgac
    38761 atgataaaaa ggctggaacc caaccttttt atgaatatcg cttatacctt aacgaatagc
    38821 ttgttcggta tctctgtcaa agaaatgccc cttagccaca ttaaaagtca agctaacttg
    38881 ctcccctgga ctatggaaat cacgcgcatc tacacgagaa gcaaactcag tttgtccaag
    38941 cttgacatag agcatcgtct cagagcccag taactcagaa accaaaactt ctgctgtgac
    39001 agttgcactt ggataagtat catgcacgat ttgtctgcta gaaatgtctt ctggacgaat
    39061 accaaaagtg acttttttcc ccttgtaacc agccgcttca agcatttttg cttgtccttc
    39121 aggaatcgca atagcaagac catcttcact cacaatgcgg ccatctttaa cttccacttc
    39181 aaagaagttc atggcagggc ttccgataaa gccggcaacg aatttattag ctggaaggct
    39241 gtacaattct tgtggcgaac ccacttgttc aattttacca atcgttccat taccctgagg
    39301 gtttttagtg gcactcatga taacgatacg gtctgccaag gtcatggctt ctgtctggtc
    39361 atgggtaacg taaatagttg ttgagccaat cctacggtga atctttgcga tttcagcacg
    39421 catggagaca cgaagcttcg catccaagtt tgacaaaggc tcatccatca agaacacttt
    39481 agcatcacgg acaatagcac gccccatggc cacacgctga cgttgaccac cagacaggtc
    39541 agcaggttta cgctctaaaa attcggtcaa accaagaatc tgcgcagctt ccttgacacg
    39601 acggtcaata tcatcttttt tgtacttacg taatttcaaa ccaaaggcca tgttatcata
    39661 cacagtcatg tgtgggtaaa gggcgtagtt ttgaaagacc atggcaatat cacggtcttt
    39721 aggagacttg tcattcacca cttcgccacc gatttttaac tctccttcag aaatgtcctc
    39781 aagacctgca atcatacgaa gagtagtcga tttaccacat cctgaaggtc caacaaagac
    39841 gataaattct ttatctttga tgtccaagtc aaaatcttca acggcataat gagtagtatt
    39901 ggggtatttt ttatagatgt gatttagatt taattcaacc atgtgtaact cctatttagc
    39961 cagcaaacgt tctagctgac tgcaaagcta gaaaccgctg atatctttta tatgatgctt
    40021 ttattataac aaaaatagcg ctttcaaaac agagacaaag cgaccaaact tctttgggca
    40081 acttgcacaa tagcagggct agtcaagtcg taagggacca taaaccaaga aaactatgta
    40141 aacacaggcc ggtcacagga gccaaaacaa gctgaacgat ctatcattct ccttatcagc
    40201 tacagtccag gcagcaaaaa agaaagccct gtggctttcc taatatttca acaaaaagag
    40261 atgagcaaag gctaaatcat ccaattgctt caagtgtagt cctgattgct gagcaaattt
    40321 atcaagtttg tattgcaagg agttacggtg aatatacaag cgctgcgctg tctgcaccag
    40381 attgccgtgt tctgaccata gcgcgtgaac cacatcggcc atatcacttt gaggacttag
    40441 gaattgttga aactgacgcg tcaatgccaa aaaagtgtgg tgcgacagca aagaccacag
    40501 catcagtcct gaaaatgtta ataattttct gcctgattgt tgccttaaat aggccgttaa
    40561 cagctgacat tcctcctcaa aacactctct caatgtaccc gcagctactt gataccaggc
    40621 atttccaaca aacatggtta aggccaaacc aaaatcactt tctagcgttg gcaagagatc
    40681 ttcaagccag cgcaagacct ttattgagac tgcttgtcgg cataaaaatg ccgtttgggt
    40741 tgcactaata ggcaaaatga cctcaagccc tgcaatcatc tgttggagca actcaactaa
    40801 gtcagctggt aaagcctgct gatgattaag gtaaatgaac tgagaagtct caaacagctg
    40861 agggggattg ccctgatgat caagcaaata ccgctgccaa ggtgacccta gcggctgcaa
    40921 gacctcacac tgatccagtc ccacctcaag taataagcgc tctttttcgg ataaacatga
    40981 tttgggaaaa tgcacatatt ggtccccttc tttgacactc acccactctt tatcccctaa
    41041 cggatgaggg cctacttgca tctctggaaa atagtctttt agctccatag ccattccttt
    41101 catgacggtc tttaaaccat tataacacat gactctttat cacacagttc ggtttgttgt
    41161 cagcattatt ttgtattttc tgactttttt aatcattaaa gctaaataat ggttattcat
    41221 ttttagcaag aacattcaat caaatagcta tttatcggaa gattaattta tgtttatact
    41281 aaaata
//
LOCUS       DAUGHY010000011        40889 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.011, whole genome shotgun sequence.
ACCESSION   DAUGHY010000011 DAUGHY010000000
VERSION     DAUGHY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40889
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.011"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            31..1893
                     /gene="cadA"
                     /locus_tag="VH752_000554"
     CDS             31..1893
                     /gene="cadA"
                     /locus_tag="VH752_000554"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEQ8288928.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITA
                     FLIGGYASAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLE
                     EMAMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQ
                     LLSPFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINL
                     VESAQEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVFSWTWLAAFYRGMILLTV
                     ASPCALIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSV
                     VNAHYLEDELLVNRLVKGAEAASTHPISKALLEYTEKLEPLTFDHLEEISGKGFQGFY
                     QGQEWRIGKKTFILEKVQDLSAFEETIQVEENQGKTLIFVSRDHQLIAYYALLDDIKI
                     ESKRAIKSLHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTK
                     YGFVAMVGDGINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKM
                     KTIIKQNIVFALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     gene            complement(2193..3128)
                     /locus_tag="VH752_000555"
     CDS             complement(2193..3128)
                     /locus_tag="VH752_000555"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEQ8288929.1"
                     /translation="MVSTATQISHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLEVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLSFVNCVNSIGNGLVIEDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
     gene            3367..4062
                     /locus_tag="VH752_000556"
     CDS             3367..4062
                     /locus_tag="VH752_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEQ8288930.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KLNIVKEYYLGGE"
     gene            4170..4691
                     /locus_tag="VH752_000557"
     CDS             4170..4691
                     /locus_tag="VH752_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEQ8288931.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQEAFLAIK
                     HDLELAGILYKGI"
     gene            4852..5394
                     /locus_tag="VH752_000558"
     CDS             4852..5394
                     /locus_tag="VH752_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288932.1"
                     /translation="MTKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFLAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
     gene            5518..6297
                     /locus_tag="VH752_000559"
     CDS             5518..6297
                     /locus_tag="VH752_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEQ8288933.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIAASPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFVTNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
     gene            7085..7681
                     /gene="recR"
                     /locus_tag="VH752_000560"
     CDS             7085..7681
                     /gene="recR"
                     /locus_tag="VH752_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEQ8288934.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYHG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            7782..8828
                     /locus_tag="VH752_000561"
     CDS             7782..8828
                     /locus_tag="VH752_000561"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEQ8288935.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEMFDQRESHLI"
     gene            9027..10418
                     /locus_tag="VH752_000562"
     CDS             9027..10418
                     /locus_tag="VH752_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEQ8288936.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELPGTPHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
                     QDHMGDIRLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAP
                     GDPIIDPYLPENQMVIRFGNQQEIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADILSDVYNANPTAMRL
                     ILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKE
                     LARLASQVYPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVE
                     RLVTDKNCDSYQP"
     gene            10499..11194
                     /locus_tag="VH752_000563"
     CDS             10499..11194
                     /locus_tag="VH752_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEQ8288937.1"
                     /translation="MDFFAIDPIGLPHISLIFYLLSLLIALLLVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLCMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYLGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATVNFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLAFAFSKEK"
     gene            11442..12986
                     /locus_tag="VH752_000564"
     CDS             11442..12986
                     /locus_tag="VH752_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEQ8288938.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            13293..14912
                     /locus_tag="VH752_000565"
     CDS             13293..14912
                     /locus_tag="VH752_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ8288939.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGGYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            15039..17039
                     /locus_tag="VH752_000566"
     CDS             15039..17039
                     /locus_tag="VH752_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEQ8288940.1"
                     /translation="MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GVNQVSESFILGSISLIIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKMSPWL
                     IVPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
                     GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNMLGHLEIFKAINPYYALHLLFSPENH
                     RGIFILGSIFLATTGAEALYSDLGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKH
                     SGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLVSEAMRLKIFPLFRVT
                     YPGANLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYL
                     IKKGTRPILAHLVMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTR
                     IVFKYVKSLNLNDYKEQIKQLRDDVCFDLYQTNVVYLSNRMQDHMIDRSILYSILDKR
                     PKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYLRTIVQD
                     LMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVSNARQLSNFERFIMQTKASIKHVT
                     ASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVPVEDSEA"
     gene            complement(17063..17341)
                     /locus_tag="VH752_000567"
     CDS             complement(17063..17341)
                     /locus_tag="VH752_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEQ8288941.1"
                     /translation="MTTKKAYMYVLECVDKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(17331..18185)
                     /locus_tag="VH752_000568"
     CDS             complement(17331..18185)
                     /locus_tag="VH752_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEQ8288942.1"
                     /translation="MKADFSKVKITLQTFTSSLFYVKITGMIKAILKEGERIDQLFSS
                     DVGIIQNKDVFSYSIDSVLLSRFPKMPSKGLIVDLCSGNGAVGLFASTRTKAAIIEVE
                     LQERLADMGQRSIQLNQLEDQVTMICDDLKNLLNHVPRSGVDLMLCNPPYFKSHESSK
                     KNVSEHYLLARHEVTTNLEEICQVARHALKSNGRLAMVHRPDRFLEIIDSLRANGLAP
                     KRVQFVYPKLGKSANMLLIEAIKDGSIEGMTILPPLVVHKENGEYTDHIFEIYFGAAS
                     KGKPNHDH"
     gene            18225..18965
                     /locus_tag="VH752_000569"
     CDS             18225..18965
                     /locus_tag="VH752_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEQ8288943.1"
                     /translation="MFYAYLRGLVVFLLWVVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKLAKVKIMPAAYQGPMSVKGLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRMDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTMLFSYIASFVWNPDKHRH"
     gene            19165..19827
                     /locus_tag="VH752_000570"
     CDS             19165..19827
                     /locus_tag="VH752_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEQ8288944.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDSSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQPIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            19808..22051
                     /locus_tag="VH752_000571"
     CDS             19808..22051
                     /locus_tag="VH752_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEQ8288945.1"
                     /translation="MTFSWTKLVPLSKIQFAFLILVLFYQIHSPSWLTFLLSLSLICL
                     LVKRLSKKEFLGVFAILSFCALFLLYQKQQLIQKLEIQPVQITSVALVPDSIRINGDQ
                     LAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
                     FLAYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLL
                     FGYLDKSFGEMTDYYSQLGIIHLFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELP
                     FACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFLWDAHFLMTVGGVLT
                     FSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTGLLSYLF
                     DLFILPLLCLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLIL
                     VISFAIFYDYRQVRQRVITCGLVIALTLLSVKYPLTNEVTFIDIGQGDSILVREWTGK
                     NLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHADTDHMGD
                     IEVVAKAIRIKEILTSQGSLSHPSFVRRLRRLKCHVRVLAAGDQLPIMGSVLQVLYPW
                     QLGDGKNNDSLVLYGRLLNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTS
                     SSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDRQITYYRTDTQGAIRLTGWTS
                     WHLETVR"
     gene            22122..23162
                     /locus_tag="VH752_000572"
     CDS             22122..23162
                     /locus_tag="VH752_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEQ8288946.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIAFDK
                     DDLAYSYFDMSEAAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTNKKSFLKEKDLKAF
                     EAYLENPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEANPLKEAELRTYFQKYSHQ
                     LGLGFESGAFEQLLLKSNDDFSQIMKNMAFLKAYKKTGNISLTDIEQAIPKSLQDNIF
                     DLTRLVLGGKVDVARDLIHDLRLSGEDDIKLIAIMLGQFRLYLQLAILAKEGKSEQQL
                     VTSLSEILDRRVNPYQVKYALKDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVD
                     IALLKIMTHSQK"
     gene            23259..23864
                     /locus_tag="VH752_000573"
     CDS             23259..23864
                     /locus_tag="VH752_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEQ8288947.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQALINNGGGHLNHALFWELLSPEKQDVTPDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIINWKKVSELYQAAK"
     gene            24031..24257
                     /locus_tag="VH752_000574"
                     /pseudo
     CDS             24031..24257
                     /locus_tag="VH752_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24242..24490
                     /locus_tag="VH752_000575"
     CDS             24242..24490
                     /locus_tag="VH752_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8288948.1"
                     /translation="MDADLAQMDALGLYYDDNHLSLVNTVKVYLKEFGIGESQIAFSY
                     KDLNSGRTAAMNDHQPMIAGSTYKLPLNMLVVDKANYP"
     gene            24487..25077
                     /locus_tag="VH752_000576"
     CDS             24487..25077
                     /locus_tag="VH752_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEQ8288949.1"
                     /translation="MTERFDITETDYEYDQEHHAYVAQFNGAMSIPDMQEYSLVYSEN
                     TPAYALAERLGGMNKAYQLFDRYGKVSGAITTIDRNGNKITTAYYLQGLDYLWQHQDK
                     YKDILYYIGESFPDLYYKTYLPHVKVYQKPGYVREALNVGAIVCEESPYLITLYSSGL
                     GGATQASEEVNGLGYVQLVQLTYVINEWHRVNHNMI"
     gene            complement(25191..26411)
                     /locus_tag="VH752_000577"
     CDS             complement(25191..26411)
                     /locus_tag="VH752_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ8288950.1"
                     /translation="MKLLGKWLFFLMFLTFCVSFWQKDYQRIAFEKEHKLYQNLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSTLIQAYYVNLEHIPYSRFNRKIHQLRYLISTQQTEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKAINSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLLHHY
                     QETRGDNKTSKTQH"
     gene            complement(26418..27446)
                     /gene="queA"
                     /locus_tag="VH752_000578"
     CDS             complement(26418..27446)
                     /gene="queA"
                     /locus_tag="VH752_000578"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEQ8288951.1"
                     /translation="MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDVKQAGGRVVAVGTTSIRTLETIGGKFQGDIQADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            27648..28352
                     /gene="nagB"
                     /locus_tag="VH752_000579"
     CDS             27648..28352
                     /gene="nagB"
                     /locus_tag="VH752_000579"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEQ8288952.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDI
                     EAEAKRYDQIIAGHPIDFQVLGIGRNGHIGFNEPGTSFEEETHVVDLQESTIKANSRF
                     FTSIEDVPKQAISMGIASIMKSKMIVLLAFGQEKADAIKGMVFGPITEDLPASILQKH
                     DHVIVIVDEAAASQLD"
     gene            28471..29187
                     /locus_tag="VH752_000580"
     CDS             28471..29187
                     /locus_tag="VH752_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ8288953.1"
                     /translation="MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
                     LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPIHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
                     CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLA
                     SGEYRYLTASERQLIKTYLD"
     gene            29482..30444
                     /locus_tag="VH752_000581"
     CDS             29482..30444
                     /locus_tag="VH752_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEQ8288954.1"
                     /translation="MTKILTALDGKNQLISLVTQPISTKPPFCCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     SLGFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLSGYNLLMLTTDAFYPQWRPVQSSSG
                     FCQIEGNLRPYYESFKVYYKKEKDKKVQTLFSPKYYVKMDSNRK"
     gene            30457..32262
                     /gene="pepF"
                     /locus_tag="VH752_000582"
     CDS             30457..32262
                     /gene="pepF"
                     /locus_tag="VH752_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ8288955.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEIDAAKDFS
                     GHLLDSSANLLTITDTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMALSQEDYQAFLAETPDLKRYHHFFDKLFQTRDHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSVRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNHFIQYEWA
                     RIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMTKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
     gene            32638..33834
                     /locus_tag="VH752_000583"
     CDS             32638..33834
                     /locus_tag="VH752_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEQ8288956.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTAAEIAILDKKRLQNNSYLIEGMTAKEALK
                     TKSFYCLWVILFINITCGLGLISVVAPMAQDLTGMSPEMSAIVVGAMGIFNGFGRLVW
                     ASLSDYIGRRVTVILLFLVSIIMTISLIFAHSSLIFMISIATLMTCYGAGFSLIPPYL
                     SDLFGAKELATLHGYILTAWAIAALTGPMLLSITVEWTHNYLLTLCVFIVLYILGLMV
                     VLRLKK"
     gene            33900..34607
                     /locus_tag="VH752_000584"
     CDS             33900..34607
                     /locus_tag="VH752_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEQ8288957.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQTTGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQIRLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEILR
                     LLKVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLTHPNLTTSLVPLS
                     DGLLMIRKNQADIVLPD"
     gene            34670..35725
                     /gene="prsA"
                     /locus_tag="VH752_000585"
     CDS             34670..35725
                     /gene="prsA"
                     /locus_tag="VH752_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ8288958.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIAN
                     ALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSS
                     AEEPTETEAQTQEPAAQ"
     gene            36112..38730
                     /gene="alaS"
                     /locus_tag="VH752_000586"
     CDS             36112..38730
                     /gene="alaS"
                     /locus_tag="VH752_000586"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEQ8288959.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYNPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKHDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAETV"
     gene            39090..39305
                     /locus_tag="VH752_000587"
     CDS             39090..39305
                     /locus_tag="VH752_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8288960.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYTP
                     SVIIAMKIAKVFQEPVEEVFRLVEVEE"
     gene            39302..40063
                     /locus_tag="VH752_000588"
     CDS             39302..40063
                     /locus_tag="VH752_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEQ8288961.1"
                     /translation="MKKKKNSFLRLLKMLLLSSLAGGIIGGMVGAFLGYHGERLDQLT
                     FLKDDVINLIILLNRLVVVTGLTLSFVFLTQLKKETAVYNTIEEDDYSENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIRGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVLSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVKSATMTRQFYR"
     gene            40082..40777
                     /locus_tag="VH752_000589"
     CDS             40082..40777
                     /locus_tag="VH752_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ8288962.1"
                     /translation="MKGFINYLKIAVLIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTPIKQQKMRLVDWGYLALFWLIIRVIAIAGTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
BASE COUNT        12515 a         7148 c         8482 g        12744 t
ORIGIN      
        1 gaataacttt taattgcgag ggaactaaaa atgacaatca gacaatggat ggcagaccat
       61 cttcatttaa tggagacttt agcgtgtttg gtattaataa taattggcct agccttttta
      121 cactcatttc cacaagtggc ttccgctatt ttcattacag ccttcttgat tggaggatat
      181 gcgtctgcaa aaacgggtat attggatttg gtgaagaaca aacacttgtc agtggatatt
      241 ttgatgattt tggcagctat cggtgctgga attatcggct attggctgga gggtgctctg
      301 cttattttta tcttttcgtt gtccaatacg cttgaagaaa tggccatgga aaaaagtaag
      361 gatgctattt cagccttgat gtccttgacg ccagatacag ctcgtcaata ccaagaagat
      421 ggccatattt tagaagttga gaccagatcc ttgagtgttg gtgaccgttt acaagttcgt
      481 aaaggcgaag ctgttccaat tgacgggcag ttgcttagtc cttttggtca atttgatgaa
      541 tctatggtca ctggtgagcc tatcactgtt gataaggcag aaggccagga tctgattggg
      601 ggaaccatta accaaggaca aaccatagat atgttggtta ctattgaaaa tgacgatact
      661 ctctttgcca agattattaa tctggtggaa tctgcccaag aaaagaaaag caaaaccgcc
      721 acctttatcg aaagcttaga agatggttat gtcaaatttg tgctcgtcct tattcctgcc
      781 tttatcctct ttagccactt tgtgttttct tggacttggt tggctgcttt ttaccgaggg
      841 atgattctct taacagtagc ttcaccatgt gccctaattg ccagttccac acctgctagc
      901 ttggctgcta tttctcgtgc agccagaaag ggattgatta tcaaaggagg ggatattgtc
      961 gataacatgg gagatattaa ggctgttgtc atggataaaa cgggaacgct cacccaagga
     1021 aaaccttctg ttgtgaatgc tcattatttg gaagatgagt tgctcgtgaa tagactggta
     1081 aaaggagcag aggctgctag tacccatcct atttctaaag cccttcttga atacactgaa
     1141 aaattggagc cactgacctt tgaccactta gaagaaattt ctgggaaagg ttttcagggc
     1201 ttctatcaag ggcaagaatg gcgaattggc aagaaaacct tcattttgga aaaggttcaa
     1261 gacctatcag cttttgaaga aactattcaa gtggaagaaa atcaagggaa aaccctaatc
     1321 tttgtttcac gtgaccatca attgatagct tactatgccc tcttggatga tatcaaaata
     1381 gaatcaaaac gtgctattaa gtctcttcat gccatgggaa tcaaaacagt catgttaaca
     1441 ggtgaccaag aacgaaccgc caattatgtg gcacaaaaac ttggtattga tgaagtggta
     1501 gccaactgta tgcctcaaga taaggtggct aagttagcag aattaaagac caaatatggt
     1561 tttgtggcca tggtaggaga tggtattaat gatgctcctg cccttgctca agcagatgtt
     1621 tcttatgcta ttggatcagg aacagatatt gcaatggaaa gtgcagacag tgtgattatg
     1681 gatgacttga ctcgtattcc attttcgatt caactttccc gcaaaatgaa gaccattatc
     1741 aaacaaaata ttgtttttgc cttatctgtg attaccttat tgattttagc caatgttttt
     1801 caggtagtta acttgccgct tggtgttgtt ggacacgaag gctcaacgat tttagtgatt
     1861 ttaaatggct tgcgtttact ttcttttaaa taatttagaa acttgcttga aaacttgaaa
     1921 gtgtttataa taatagtaag acttatgaaa gagaaaaccg ccgtatgttt actattatga
     1981 tcattggttt agctgtattt tcaataatta aaatcattct atttttctat ttttatcagc
     2041 aatttgagcg caagcaaaaa agaaaagttg aatcaaacaa ccagcaaaga cagtcccaag
     2101 aaagtgacgc tgtcccttca aaaaaattcc cattttaaaa agcttggaaa gtgtacaaag
     2161 tgcacatttc caagcttttt tgactattaa ggttaatctt tgtaacgtaa gttcccacga
     2221 aaatcgtcta agctttggta gcctttttct accataatcg tttttagttc cttagtgacc
     2281 cgttcaaaga tagctggccc ttcttgatga agggctgtcc caatttggac catgctggcg
     2341 ccacaaagaa tatgttcaaa ggcatcgcgt cctgttttaa ctccccctgt accaataatg
     2401 tggatagaag gttttaagcg tttgtaaaac gcatggacat tggctagagc agtaggtttg
     2461 atgtaatctc cacctatacc accaaagcca tttttaggct tgatgaggac ttgttcatcc
     2521 tcaatgacca gcccatttcc gatagaattg acacagttaa caaaggaaag ggggtattta
     2581 ttgaaaatcg cagccgcttg atcaaaatgc acaatgtcaa aataaggagg taatttgata
     2641 ccaagaggtt tagtgtaata ggtaaagatg ttctctagaa gttgatcagt ggtttcaaaa
     2701 tcataagcaa tctgtggttt tcctggaacg ttagggcaag aaaggtttag ttccactaga
     2761 ccctcataat ctgaagccat gatggctttt aaaatggttt cggtttcagt aggagatagc
     2821 ccgacaacgg agagaaagtg aggtttatgt tgcccggttt tggcaagatc actgacaaag
     2881 tctaagtagt agcgaaaacc attattaggg agtcccattg aattgataga accaagacga
     2941 gtatctgcgt agcgaggttc tggattacct ggacgaactt caagcgtccc tgttttggtg
     3001 acaaaggaag cggcttgaga cttttcaacc tccataagct cttcttttgt catgcagtaa
     3061 acaccagcag cattcatcaa acagttatca aaagagaaat gactaatttg tgtagcagta
     3121 gaaaccatta tttcctccat attttagaca aattttttct attctaccac taggcatgaa
     3181 taatagcaag ctatttttac aaaaatgtaa atatattact gttattgacg aacatttgaa
     3241 attttcatga tagtcacatt atgattttca ataaagtcag aatagactag cttttttgct
     3301 atattttttg taaaatagga attgtaaaaa ggtagcaacc tttaaattaa aaggagatat
     3361 taactcatgg taaaattggt tttcgctcgc cacggtgagt cagaatggaa caaagctaac
     3421 ctcttcactg gttgggcaga tgttgatctt tcagaaaaag ggacacaaca agcgattgat
     3481 gcaggtaaat tgatcaaaga agcaggtatt gaatttgacc ttgctttcac ttcagtattg
     3541 acacgtgcta tcaaaacaac taaccttgcc cttgaaaatg caggtcaatt gtgggttcca
     3601 actgaaaaat catggcgctt gaacgagcgt cactatggtg ctttgactgg caaaaacaaa
     3661 gctgaagctg cagaacaatt tggtgatgaa caagttcata tctggcgtcg ttcatacgac
     3721 gtgttgccgc cagctatggc taaagatgat gagtattcag cacacaaaga ccgtcgttat
     3781 gctgatcttg acccagccct tattccagat gctgaaaact taaaagtaac tcttgaacgc
     3841 gcaatgcctt actgggaaga aaaaattgct ccagctcttc ttgacggtaa aaacgtcttt
     3901 gttggtgcac atggtaactc aatccgcgct cttgtgaaac acattaaagg tctttcagat
     3961 gacgaaatca tggatgtgga aattccaaac ttcccaccac ttgtttttga attagatgaa
     4021 aaacttaaca ttgttaaaga atactacctt ggtggtgaat aacttaaaaa ccagactttg
     4081 gtctggtttt ttgggttcta tatcctaaga agaaagaaaa ccctgcttaa aaatatgata
     4141 tgatagaaat aaaaaaggat agatagatta tgaaagcaga agatcgtcgt caaaaaatta
     4201 ttgagtgctt aaatagtgaa caaaaagctg tatctgctac tcgtttaggt aagttattag
     4261 gagtgagtcg ccaagtaatt gttggagata tagctcttct tcgagctcag cagattgata
     4321 ttatttcgac tccaaaaggt tatattatgt caacagcgct ttatagtcac caattttgtg
     4381 cacgtattgt gtgccaacat aatgtagaag aaactaaaaa agaattagaa attattttag
     4441 ctcatcaagg gattattacg acggtagagg tagaacatcc tatttacggt atgataacag
     4501 cccctttaaa tattaagaca cactctgatg taactaattt catgtccaaa ctcagtcaat
     4561 ctaaagcaga gttattgtct tcgttgacag aaggactaca tagtcactta atttcgtgtc
     4621 ctagtcaaga agccttttta gctatcaagc atgatttaga attagcaggt atcttataca
     4681 aaggaatata actacagtat attatgccat tgatggttgt tatgaatacc atgctaaaat
     4741 attctattga ttatttatca gcaacaaggt atcgtttaaa aataccaaaa gatacaggtg
     4801 tcttgatagt tattggtttt taaggtatac tatttttgaa aaggagataa aatgactaaa
     4861 aaaccaacac aactaatagc ttatacctct attttggtcg catttgcgat tttgattccg
     4921 ataatcatgc ctcttaagct tattattgga ccagcttcct ttactctggc gagccatgtt
     4981 cctttgtttt tggctatttt tatgtcgatt ccagtagcaa ttttggtcgc tcttggtacc
     5041 acattaggtt ttttattagc tggacttcct ttaattattg ttttaagagc actctctcat
     5101 ttattgtttg ctattttggc agcgtggtgg ttaagtagaa aaccacaact gatgacatcg
     5161 gcagtaaaat gttttagttt tgcgtttttc attaatgtca ttcatggctt ggctgaattt
     5221 ttagttgtct atattttaac agcaaccaca gcaacttcta tgtcttattt ttggtctatg
     5281 ctaggcttga ttggcttagg ttcgcttatt catggcatat tggatttcta tcttgcttta
     5341 gttctatggc gttttttagc aaaaaactta aaacttccca ccataaatgc ctaagactaa
     5401 aatagagata gttatatgat aatgacgagc cctttcttgt tgaaagggct tgcatttttt
     5461 aggaaaattg atatactaaa gtgtgtgttt gtgaatacag taacaaaagg agatatcatg
     5521 aaaacaccag aacagatttt agaagcaact attcatattg gggaacataa ggttaccaaa
     5581 acttttttgg ctaaatctat cttaggcttt attggtggag cgatgattag ccttggttat
     5641 ttactatacg tgagaattgc cgccagtggt ttagaaactt ttggtgcttt ttccagtatc
     5701 gtaggagctt gtgcttttcc gattggtcta attattattt taatggcggg tggagaatta
     5761 attacaggga acatgatggc tgtttctgca gctttgttgg ccaaaaaaat taagtttagc
     5821 gagcttgcca agaattggct tatcattacc ttgtttaatg tgattggagc tgtttttgta
     5881 gcttttgtgt ttggtcattt tctaggcttg acatcagctg gtatttttaa agaagaagtg
     5941 attgaggtgg cccatgctaa aatagcagcg agccctttgc aggcactagt atcaggaatt
     6001 ggttgtaact ggtttgttgg tttggccttg tggctatgct atggggcaaa tgatgctgct
     6061 ggtaaattcc ttggtacttg gtttcctgtc atgacctttg tagcccttgg ttttcagcat
     6121 agtgtagcaa atgcttttgt cattcctgct gctatttttg aaggaggagc aacatggttg
     6181 gattttgtca ctaattttat ttttgtctat agtggtaata ttatcggtgg agccattttt
     6241 gttagttttt tatattttaa agtttactat catccgcaaa agagtaaaac tcaatgagaa
     6301 aatatttttt tagactgagg gcgtcctatt ctcagtcttc tttttataat ttatggtaaa
     6361 ataagaagcg aagtttaaaa aatttagcta aatatggtta gtttattccc taaaaaagga
     6421 gtattcccaa aaaatccgta aaaagaattt gctatgggag tagttcccaa aataatgcag
     6481 atcattagtt gtctttcaaa aatagccttg atagggtaaa aagtggacag tgatgcagca
     6541 acagattcat caggcgtaca aactgtctta tggtttgtgc ttagagattt cttaataatg
     6601 cttttggtaa tacactttta gtgaagaaag tgtagcatga aagcttaaag ataaccatga
     6661 gtttgcttta tgcactattg ttgagaaaaa tagcatagtc aattgagtaa acatcatttc
     6721 tgcatggaat gaaacaattc ctataaactt atatcataag gatggtttgc ggtttctaaa
     6781 aaagtaacat gaatcaaaaa acaaaagcca aatatcccaa ggccaaggta acaagtcgag
     6841 taaaaaagtt tttacttggc taatccaaac ggttctgtca cgattcacgc taaaattgga
     6901 actgctgaga cttgtatcac agacaattca gatcagacct tagtaactta caacttaaat
     6961 gtagtagctt ataatacaaa taacgaagat aatattgcgg ttgctgtaca tattacgaat
     7021 atatcggcaa agatatgagg gcgctttatg tggcgatata tggaaaacaa taaaggagaa
     7081 cttagtgctt tatccaacac ccattgcaaa gttaattgac agttactcta aacttccagg
     7141 aattggtatc aagacggcga cgagattagc cttttatact attggaatgt caaatgaaga
     7201 tgtcaatgat tttgctaaaa acttattagc agctaaaaga gaactgacct attgttcgat
     7261 ttgtggaaac cttaccgatg acgatccttg tcacatttgc acagacacga gccgtgatca
     7321 gacgaccatt ctggtagtag aagatgctaa agatgtttct gccatggaaa aaatccaaga
     7381 gtatcatggc tattatcatg tgcttcacgg cttgatttcg cctatgaatg gtgtggggcc
     7441 agatgacatc aaccttaaaa gtttaattac ccgtttaatg gatggtaagg tgagcgaagt
     7501 tatcgtagct accaatgcca cagcagatgg ggaagcaacg tccatgtata tttcacgtgt
     7561 cttgaaacca gccgggatta aggtaactcg tttggcaaga ggtctcgcag taggttcaga
     7621 tattgagtat gctgatgaag taacattatt gagagctatt gagaatcgta ctgaacttta
     7681 atgatcatgt tggtgccttt actcgcaaag agaggttttg aaaaggctac atctttttgt
     7741 tataataaaa aaacattcaa ttcagataag aaaggttagc tatgtccaaa caaacgttag
     7801 ttctattata cggaggccgc tcagcagaac gtgaagtctc agtgctttca gcagagagtg
     7861 tgatgcgagc agtcaactat gataagttct tagtaaaaac ttattttatt actcagatgg
     7921 gccaatttat caaaactcag caattttcag aaaagccatc ggaatcagaa cgcttgatga
     7981 caaatgagac gatagagcta actcaaaaaa taaaaccaag tgatatctat gaagaaggtg
     8041 cagttgtatt tcctgtatta catggaccta tgggagaaga tggctctatc caaggtttct
     8101 tagaagtgct tagaatgcca tatataggga ctaatgtcat gtcttctagc atagctatgg
     8161 ataaaattac tactaaacgt gtcttagaat caattggaat tccacaagtt gcctatactg
     8221 tttatattga cggacaagat ttggaggctt gtttagttga aactttagca agacttactt
     8281 ttcctatctt cgtaaaacca gctaatatgg gttcttctgt ggggatttcc aaagcccaaa
     8341 caaaagtaga attaagaaaa gcgattcagt tagctctgac ctatgacagt cgtgtcttga
     8401 ttgaacaagg cgtggttgcc agagaaattg aagttggtct ccttggaaat gataaggtta
     8461 aatcaactct accaggagaa gtaatcaagg acgttgattt ttacgactac caagcaaaat
     8521 atgttgacaa taagattacg atggccattc cagcagatgt agaccagagt attgtgacag
     8581 aaatgcgttc ttatgctgaa gtagccttta aagccttagg tggttgtggg ttgtctcgtt
     8641 gtgacttttt cttgacccaa gacggacagg tttaccttaa cgaattaaac accatgccag
     8701 gatttaccca atggtctatg tatccactct tatgggaaaa tatgggattg gcttatccag
     8761 atttgattga agagctggtg acactggcac aagaaatgtt tgaccagcgt gagagccacc
     8821 tgatttaaac ctatgagatg aataaatatt gacttggatg gaacggcaaa catcgtcaag
     8881 atgtgacgtt cgtccttttt ttgttgctct tttaggaaga atagtagagc cattaaggga
     8941 gtttgacatt ttagaaattt ttctaatgtt ttgtgctata atagatagga catcgtaaat
     9001 actagtgttc ttagaaagaa aacaaaatga aattaacctt acacgaagtg gctaaaatag
     9061 ttgacgccca aaataatgtg tctgacttag acgacgttcc tctgcatcac attgaatttg
     9121 atagccgaaa gattacaaaa ggagacctct ttttaccctt aaaaggtcaa agggatggtc
     9181 atgagtttat tgatttagcc tttcaaaatg gagctgtcgc tactttttca gaaaaagaat
     9241 tgccggggac acctcacctt cttgttgaag actgcctaaa agcttttcaa aagctagccc
     9301 attattatat tgataagatg cgcgtggatg ttattgctgt gacaggctca aatggtaaaa
     9361 cttccaccaa agatatgata ggagctgtcc tatcaaccac ttacaaaact tacaaaacgc
     9421 aaggaaatta caataatgag attggtttgc cttacaccgt tctccacatg cctgacgata
     9481 cggaaaaaat tgttcttgaa atgggacaag atcacatggg agatattcgt cttctgtctg
     9541 aaattgcaag gcctcgtatt gcagttttaa ccttagtcgg cgaagctcat ttagaatatt
     9601 ttggaagccg agacaaaata gcccaaggca aaatgcagat tgttgacggc atgaattcag
     9661 atgggatttt aattgctccg ggagatccga ttattgaccc atatcttcca gaaaatcaga
     9721 tggttatccg ttttggaaat cagcaggaaa ttgacgttac tggtattcaa gaagacaaag
     9781 atagcttaac gtttacaaca aatgtgttag caacacctgt gtccctacct ctaccaggaa
     9841 aatacaacgc gaccaatgct atggtagcag cctatgtagg aaaactattg gcagtaacag
     9901 acgaggatat tatagctgct ttacagactg tgacgttgac aggtaatcgt acggagtgga
     9961 aaaaagcagc caatggtgct gatattttat cagatgtcta caatgccaac ccaacagcca
    10021 tgcgtctgat tttagaaaca tttgctaaca tagcaaagaa ccccggagga aaaaagattg
    10081 cagtgctagc agatatgaaa gaacttggaa aggattcagt gatacttcat tctcagttaa
    10141 ttgacagctt aacatcaggt aatattgatc agcttgtttt ttatggggat catattaaag
    10201 aattagcacg gttggcaagt caggtttatc ctgctgagca agtacattat tttttaaaaa
    10261 ctgaacaaga agaccagttt gaagccatgg cacaatatgt tcaaaacatt cttaatccct
    10321 ttgatcaaat tcttttaaaa ggcagccact cgatgtcgct agaaaagtta gtggaacgat
    10381 tagtgactga caagaattgt gacagctatc agccttgatt tttataaaac tttcagagaa
    10441 aggcttgttc tctcatgaag attaaggtat aatgaaaaaa atatagaaag ggacgattat
    10501 ggattttttt gcaattgacc ccatcggact gcctcatatc tcacttatct tttacttgtt
    10561 gtcactcttg atagcgcttt tgcttgtttt tttgactttt caagcatata ggttaaaatc
    10621 ttatcgttat tttttcttgt ttttacagtt atctcaggtg attggcttgt acacatggta
    10681 cgtgttaagg gggtttcctt tagatgaagc tttgccgttg tatcattgtc gcatcgctat
    10741 gttagctatc ttttttctac ctgatcgtaa caagtttaag caattatgta tggttttggg
    10801 gataggtgga acattccttg cacttttatc gccagatctt tatccgttta gattatggca
    10861 tgtagcaaat gtctcgtttt atcttggtca ttatgctttg ttagtgaatg gtttgattta
    10921 cctcttgcgt ttttacgatg ccagtcagtt aagactgcta tcagtagtac gctatttagc
    10981 aactgttaac tttcttcttc tgttggtcag tttagccacg aaaggaaatt atggttttgt
    11041 gatggatatt ccagtgattc atacgcgtca tttgctactt aattttgtga tcgttacaag
    11101 tggcttgact tttatggtta aaataaccga atatttttac cttaaatttg gtgaagctca
    11161 acagctggca tttgctttct ctaaagaaaa gtaggtcgtt gaaaaagcac ttaaaatcag
    11221 ctataataag aagagtctgg gatgatttcc aggctcgatt tttttaaaaa agacactcat
    11281 tgatagccca agataagtta taataccagt tttgaagaac tttagaggct aatcgtatat
    11341 ggagaaaccc aatgtagccc aactcttttg ataaacaaat aatacctaac gaaaataact
    11401 cagagaaaag ctctgccatt ttactataca aggaaaaaag aatgtcactt acagaagaaa
    11461 ttaaaaaacg ccgtactttt gccatcatct ctcacccgga tgctggtaag acgactatta
    11521 ctgagcaact attatatttt ggtggtgaaa ttagagaagc agggacagta aaagggaaaa
    11581 aatcaggtac gtttgcaaag tccgactgga tggatattga aaagcaacgt ggtatctctg
    11641 ttacttcatc tgttatgcaa ttcgattacg cgggtaagcg tgttaatatt ttggatacac
    11701 ctggacatga ggatttctcg gaagatacat accgtacctt aatggctgtg gatgcggctg
    11761 tcatggttgt agattcggct aaaggtattg aggcgcaaac gaaaaaactt tttgaagttg
    11821 ttaagcatcg taatattccg gtttttacgt ttattaataa attggaccgt gatggacgtg
    11881 agcctttaga actcttggaa gaattagaag aagttttagg gattgcttct tatccgatga
    11941 actggccgat tggtatggga cgcgcctttg agggcttata tgatttacat aataaacgtt
    12001 tagaactcta taagggagac gagcgttttg caagtattga agacggtgat cagctgtttg
    12061 ccaataatcc cttttatgaa caagtcaaag aagatattga acttcttcaa gaagcaggta
    12121 atgacttttc agaacaagct attcttgatg gtgacctaac tccagtcttt tttggctctg
    12181 cccttacgaa ctttggagtt cagacttttc ttgatacttt cttggagttt gccccagaac
    12241 ctcatggtca caagactaca gaaggtaatg ttgttgaccc cttagctaaa gatttttcag
    12301 gttttgtctt taaaattcaa gctaacatgg accctaaaca ccgtgaccgt attgcttttg
    12361 tgcgtattgt ttctggtgaa tttgagcgcg gtatgggagt taatttgaca cgcactggca
    12421 aaggcgctaa gctctccaat gttactcaat ttatggcaga atcacgggaa aatgtgacga
    12481 atgctgttgc tggggatatt atcggggttt atgatacagg aacttatcaa gttggggata
    12541 ccttaacagt tgggaaaaat aaatttgaat ttgaaccctt accaactttt accccagaaa
    12601 tcttcatgaa ggtttctccg aaaaatgtta tgaagcagaa atccttccac aaaggtattg
    12661 aacaattagt acaagaagga gcaattcagc tttataagaa ttatcagaca ggcgaataca
    12721 tgctgggtgc tgtgggtcaa ctgcagtttg aagtctttaa acatcgtatg gaaggtgaat
    12781 acaacgctga agttgtgatg acaccaatgg gtaaaaagac tgttcgctgg atttcagaag
    12841 atgatttgga tcaacgcatg tcctcaagcc gaaacatttt agctaaggat cgttttgacc
    12901 aaccagtttt cctatttgaa aatgattttg cccttcgctg gtttgcagac aaatacccag
    12961 atgtcacttt agaagagaaa atgtaaccga aacatgattt tcaacagtct aacgttttgg
    13021 agatcaattt actgagtaac tttttaaaag aaatgagtgg ctagttatct cttttgcaga
    13081 tttaagattc tatcaaaaaa cgatatcttc acaactgtaa gcatatttat gatttagcgg
    13141 ataatcgtga aagcgtgtta ttgcttatat tagccttatt tcctatcttt ttgattctaa
    13201 ttgtgttaca ctagttaggt tgttaatcaa acgacgaaaa ttaaaaggtt tataaggcag
    13261 cttcgcactg cctttttaga aaagagaaac aattgaaatt tacagaattt aacttatcac
    13321 aagacatcca atcagctgtt gttaccgctg gttttgagaa agcatctcct atccaggaaa
    13381 tgacgattcc tctcgcacta gaagggaagg atgttattgg tcaagcacaa acaggaactg
    13441 gtaaaacagc agcgtttggt ctaccaacct tgaataagat tcgtactaac gaaaatatca
    13501 tccaagcatt ggtcattgca ccaacgcgtg aattggctgt acaaagtcag gaagaattgt
    13561 tccgttttgg acgtgaaaaa ggggttaagg ttcgctctgt ttacggtgga tcaagtattg
    13621 aaaaacaaat caaagccctt aaatcaggtg cccacattgt cgtgggaaca ccgggtcgct
    13681 tgttagattt gattaagcgc aaggctctta ttttagatca tgtggaaacc ttaatccttg
    13741 atgaagcaga tgaaatgctt aacatgggat tcttggaaga cattgaagct attattagtc
    13801 gtgtcccagc agaccgtcaa accttgctct tctccgcaac gatgccggca cctattaaac
    13861 aaatcggggt taaattcatg aaagaccctg aacatgttca aattaagaac aaagaattaa
    13921 caaacgtcaa tgttgatcaa tactatgttc gtgtgaaaga acaagaaaaa tttgatacta
    13981 tgacccgtct gatggatgtt aaccagccag aattatccat cgtttttggg cggactaagc
    14041 gccgagttga tgaaattaca cgtggcttga aacttcgtgg tttccgtgcc gaaggaatcc
    14101 atggtgatct tgaccaaaat aaacgcttac gtgttatccg tgatttcaaa aacgaccaaa
    14161 ttgatattct tgttgccact gatgtagcag cgcgtggcct tgatatttca ggagttaccc
    14221 acgtttataa ctacgatatt acccaagatc cagaaagtta tgttcaccgt attggtcgta
    14281 caggtcgtgc cggtaaatct ggtgagtcta tcacttttgt ctcaccaaat gagatgggct
    14341 acctctcaat gattgagaac ttaacgaaaa aacagatgaa acctcttcgt ccagcaactg
    14401 ctgaagaggc ctttcaagcc aagaaaaaag tagccctcaa gaaaattgaa cgcgactttg
    14461 cggatgagac tattcgttca aactttgata aattcaaagg cgatgctgtc caattggctg
    14521 ccgaattcac tcctgaagaa ttagctttat atattttgag cttaacggtt caagatccag
    14581 atagcttacc ggaagttgaa attgcacgtg aaaaaccatt accattcaaa tatgtgggtg
    14641 gtggtcacgg caataaaaat ggcaaaggtg gtcgtggacg tgacaaccgt aaccgtggtg
    14701 atcgccgtgg cggctatcgt ggtgaccgta acagagatga gcgtgatggt gaccgtcgtc
    14761 gtcaaaaacg tgataagcgt gatggtcacg atggcagtgg caaccgcgat ttcaaacgta
    14821 aatcaaaacg taatagtaaa gactttttca ataaagagaa aaaatcaagt gctaaaaata
    14881 ctggttttgt gattcgtcac aaaggtgaat aagcttaaag agagttgatg agagtcagct
    14941 ctttttaatt ttctgaaaaa gttgctttta taatgggatc tgttataatg gtttcagtta
    15001 tttcattata atttttaaaa aattaggagg agtataagat gtctgatagc catcttactg
    15061 cctttgacaa agcttcaaaa gcaggtttta ttattgcttt aggtattgtt tatggagata
    15121 ttggaacaag ccccctttat acgatgcagt ctctagttga aaatcaagga ggggttaatc
    15181 aggtttctga atcctttatt ttaggatcaa tctctttaat tatttggaca ctgaccttga
    15241 tcacaacaat caagtacgtt ttgattgctc taaaagctga taaccaccat gaaggtggga
    15301 ttttttcttt attcaccttg gtacgtaaaa tgtcaccgtg gctcattgtt ccggcgatga
    15361 ttggaggtgc aacacttttg tctgatgggg ctcttacacc tgcggtaaca gttacttcag
    15421 ctattgaggg cttgaaagcc gttcctggtc tgagtcacat ctaccaaaat caaaccaatg
    15481 tcattatcac aactctagtg attttaatag tcttgtttgg tattcagcgg tttggaacag
    15541 gttttattgg taaaattttt ggaccagtta tgtttatctg gtttagtttt ctaggtgtta
    15601 gtggtttttt taatatgttg gggcatctgg aaattttcaa ggctatcaat ccttactatg
    15661 ctctgcattt gctgtttagt cctgaaaacc atcgtgggat ttttatcttg gggtccattt
    15721 ttcttgcgac aacaggagct gaagcccttt attcggattt aggacatgtt ggtcgaggaa
    15781 atatttatgt gagctggccc tttgttaaga tgtgtattgt gctgtcttac tgtggccagg
    15841 ctgcgtggat tttagcgaat aaacactcag gtattgaact aaaccctttc tttgctagtg
    15901 tgccatcaca attaagggtt taccttgtta gtttggcaac cttggcagct attattgcct
    15961 cacaggctct tatttcaggc tcctttacct tggtttcaga agccatgcgt ttgaaaattt
    16021 tccctttgtt tcgtgtgact tacccagggg caaatttggg acaattgtat attcctgtca
    16081 ttaactggat tttatttgca gtgacctctt gtactgtttt ggcttttaga acatcagccc
    16141 acatggaggc agcttatggt cttgctatta ccatcaccat gttgatgaca acaatcctct
    16201 taaagtatta cttaatcaag aaaggaacga gacccatctt ggctcattta gtgatggctt
    16261 tctttgccct tgtagaattc attttcttct tggcttcagc tatcaaattt atgcacggtg
    16321 gctatgcggt tgttatcttg gcacttgcaa ttgtctttgt catgtttatc tggcatgcgg
    16381 gcacacggat tgttttcaag tatgtgaaat ctcttaattt gaatgattac aaggagcaga
    16441 ttaaacaact tcgtgacgac gtttgttttg acctctacca aacaaatgtt gtctacctat
    16501 ccaatcgtat gcaggatcat atgattgacc ggtctatttt atactccatt ttagacaagc
    16561 ggccaaaacg cgctcaagtt tattggtttg taaatgtcca ggtaactgat gaaccttata
    16621 cagctaagta caaggtagat atgatgggaa cggattatat ggttcgagtc aacctttatc
    16681 ttggttttag aatgccacag actgtgccac gctatttgcg cacgattgtt caggatttga
    16741 tggaaagtgg ccgtttaccc aaacaagaac aggaatatac catcacacct ggtcgtgatg
    16801 ttggtgattt tcgctttgtt ttaatagaag aacgggtctc caatgcacgt caattgagta
    16861 actttgaacg ctttattatg cagactaagg caagcattaa acatgttacc gctagtccaa
    16921 tgcgttggtt tgggcttcaa tattcagaag taacccttga agtggttccc ttgattctat
    16981 cagatgtctt gaagttacca atcaaagaat tggtgcctgt ggaagacagt gaagcataaa
    17041 aaaatcattg agatgataag tctcaatgat ttttcttttc tttttgatga gtagcaatat
    17101 aggccaattt ctggcttctt gtttttcgtt ttttaaagag tgcttcagcg ctcatcgcag
    17161 cctctttgct atcaaacact tcgtaataaa gtaaagaaac aggtaaccgg tagcgagtat
    17221 atttagcccc ctttcctgcg ttatgtgtag ctaagcgttt tttcaaatca gttgtataac
    17281 cagtgtagag ggttttatca acacattcta acacatacat ataagctttt ttagtggtca
    17341 tgattaggtt ttcctttgga agcagcacca aaataaatct caaaaatatg atcagtgtat
    17401 tctccgtttt ccttgtggac aactaaagga ggtaaaatag tcatcccttc aatagaaccg
    17461 tctttgattg cttcaataag taacatatta gctgatttac ctaatttagg atagacaaat
    17521 tgtacccttt taggggcaag accattagct ctaaggctat ctataatttc caagaatcga
    17581 tctggtcgat gaaccattgc cagacgacca tttgatttaa gggcatgacg ggctacctga
    17641 caaatctctt ctaaattagt ggtcacttca tgacgagcca gtaaataatg ttcagagacg
    17701 ttcttcttag aactttcgtg acttttaaag taagggggat tgcagagcat caaatcaact
    17761 cctgaacgtg gcacatggtt taggaggttt tttaaatcat cacaaatcat ggttacttgg
    17821 tcttccagtt gattgagctg gatagagcgt tgtcccatat cagcaagccg ttcttgtaat
    17881 tcgacctcaa tgatggctgc cttggttcta gtgctagcaa ataagccaac cgccccattt
    17941 cctgagcaca aatccacaat taaccctttg gaaggcatct tggggaagcg ggataagagg
    18001 acgctatcaa tagagtaact aaagacatcc ttgttttgaa taatgccaac atcacttgaa
    18061 aagagttgat caatacgttc gccttctttt aagattgctt ttatcatacc tgttatttta
    18121 acataaaata ggcttgaggt gaatgtttga agagtgattt tgactttcga aaagtcagct
    18181 ttcatgatac aataagtaag attattctat taaaggagaa gcctgtgttt tacgcttact
    18241 tacgcggttt agtagtcttt ttattatggg ttgtcaatgg caatgcccac taccatcatg
    18301 aagaaaagat gttagatgct tctgagaatt atattctggt agcaccccat cgaacctttt
    18361 gggatccagt ttacatggca tttgcagcac gtcctaagca gtttattttt atggctaaaa
    18421 aagagttgtt tgcaaatcgt ctctttgctt ggtggattaa aatgtgtggt gcttttccta
    18481 ttgatcgtga caaaccgagt ccagatgcta ttcgttaccc tgttaatatg ctcaaaaaga
    18541 gcaatcgttc tcttcttatg tttccaagtg gaagtaggca ttcgcaagag gtcaaaggag
    18601 gggtcgctgt gattgctaaa ttggctaagg tcaagattat gccagcagcc tatcaggggc
    18661 ctatgtcggt caagggatta ctagcgggag aacgggttga catgactttt gggaatccca
    18721 ttgacgtctc agatattaaa cgcatgaatg atgaagggat tgctgaggtg gcaaaccgta
    18781 ttcaggcaga gtttgatcgg atggatgacg agttggcacc tttccaacca ggaaaagcaa
    18841 ggaacccttt aacttatctc taccgtttac cattaggatt ggttttggtg gtggtattat
    18901 tattaaccat gttattttcc tatattgcta gttttgtttg gaatccagat aaacatcgcc
    18961 attaagaaca tgagggctaa catgtgcctg aaataagaag tgattgagat tagtcttcta
    19021 ttgaaagaag ccgctaatgc ttaatcactt ttttcatttc taaaaaagat attcgatgat
    19081 acccttaact cttttaagca caaaaaatga caatactact atacttttgg atatcattta
    19141 cgaataaaaa gatgaggtaa aaaaatgtta gacgaattat taattaaagg aaaatcgttt
    19201 ttgagagagc ggtattttct gccataccta attagtcttt tattaggact ttttctaata
    19261 ctaagcttca gttttttgtt ctggggaaat aggcagagca aggcagccgt tcctgctttg
    19321 agagaaatat cgcctgtgaa acagcaggtt agtgaagaaa aaaaggaaat tcaagaagat
    19381 agcagtattt tagtggattt aaaaggcgcc gtacaaaaag agggagttta taagctaact
    19441 gctagcagtc gtgtgcgaga tgtcattgaa ctagctggag gcctaacttc agaagctgat
    19501 aaacatgcta ttaattttgc agaaaaacta actgatgagc aagttgttta tgtacctaaa
    19561 caaggagaag agatttctgt gctaccaaga tcattagttt ctggtaaaaa agaaactgcc
    19621 agcaaggatc agtcaaaggt tcatattaat aaggccagct tagaagaact acaacctatc
    19681 ccaggtattg gtgctaaaag agctcaggat attattgata tgagagataa gttaggtggt
    19741 tttaaagcgt tagaagatct ccgtcaggtt tcggggattg gcgaaaaaac gctcgagaaa
    19801 ttaaaagatg acattttctt ggactaagct agtgcccctg tcaaagattc aatttgcttt
    19861 tttaatcttg gttctctttt atcaaatcca tagccctagc tggttaacgt ttctccttag
    19921 ccttagtttg atttgcttac ttgtcaagcg tttgtctaaa aaagaatttc tcggtgtctt
    19981 tgccatcctt agtttctgtg ctttgttttt gctgtatcag aaacagcagt tgattcaaaa
    20041 actggaaatt cagccagtac aaataacctc tgtagcatta gttcctgata gtattcggat
    20101 taatggtgat caattagctg ttcttggacg tcacgggaaa cattcttatc agttatttta
    20161 ccgtttgaaa agccaagcag aagcccaatt gttcaagaaa gagcaccgtt ggttagtgat
    20221 gcacgcaaaa gtgacattgg aaaaagctga ggaagttcga aattttaaag gctttaatta
    20281 tcagactttt ttggcttatc aaggaattta caggataggg aaggtagagc aaatagaaca
    20341 gcttgaagtg atttcacccg agtcaatatg tgactatttg tcctctttaa gacgacgagc
    20401 cattgttcat tgccagcagc attttccaag acctatgtct cactacctga cgggcctcct
    20461 ttttggttat ttagataagt cgtttggtga gatgacagat tattatagtc agttgggtat
    20521 cattcatctc tttgctttat cagggatgca ggttggcttc tttcttactt gttttagaag
    20581 ggtgttgcta ttactcgcag ttcctttgga atggataaaa tggatagagc ttccttttgc
    20641 ttgcttttat gccgcgttga caggttatag tatttctgtt attcgaagcc tcgtgcagtc
    20701 gcagttgcgt catttaggaa taaaaggatt agacaatctg gcttgtactt ttttacttgt
    20761 ctttttatgg gatgctcatt tcttaatgac tgttggtggc gtattaacgt ttagctatgc
    20821 ttttttgtta acagtggtta ctgttgaaga attatcagga gctaaaagac agttggtaca
    20881 agtcttgacc atttccctag gaatattacc atttttgctc ttttattttt ctagttttaa
    20941 ccccatgtca atggtcttaa cagggctctt gtcttatctc tttgaccttt ttattttgcc
    21001 ccttctttgt ctggtttttt gtctatcccc tttggtgact gtctcaatat gtaaccatct
    21061 ttttatttta ttagaaaaag tcattcaatt tctcggaaat actttcaata gttcccttgt
    21121 ttttggaagc ccgaccagtt ggcacttgct tatcttggtg attagcttcg ctatttttta
    21181 tgattatcgg caggtgcgac aacgagttat cacttgcggt ttggttatag cgttgacgct
    21241 tttgtctgtc aaatatcctt tgaccaatga agtgaccttt atcgacattg gccagggaga
    21301 tagtattttg gtgagggagt ggacagggaa aaatctctta atagatgtag gtgggcgccc
    21361 tttcttttct tcaaaagagc actggcgacg aggacatcac gtagctaatg ctcagaaaac
    21421 cttaattcct tacttaaaaa gtagaggtat tcacacaatt gaccaactat tagtaactca
    21481 cgcagatact gatcatatgg gggatatcga agtggttgcc aaagctattc gaataaagga
    21541 gattttgact agtcaaggca gtttaagcca tccaagcttt gttaggcggc taaggcgttt
    21601 aaaatgtcac gttagggttt tagcagctgg agaccagttg cctattatgg gaagtgtctt
    21661 acaggtactc tacccttggc agttagggga cgggaaaaat aacgattctt tggtacttta
    21721 tggcaggctc ttaaatcgaa cttttctttt cacaggtgac ttggaaaaag agggggaaaa
    21781 cgagatcata aaacgttatc ctcaactaag agtagattat ttaaaagcag gtcaccatgg
    21841 atcaaatacc tcttctagtg cagctttttt agaccatatt cagcctaaag tggcctttat
    21901 ttctgctgga aaaaataacc gttatcagca tcctcacaga gaaaccttag cccgtttgga
    21961 agacagacaa attacttatt accgaacgga tacgcaagga gctattcgtt tgactggttg
    22021 gacaagctgg catcttgaaa cggtccgtta gttaaaattc cagcctgacg aagaaacaag
    22081 cagtcgcttt ccttttagga gaaagatgat ataataaaag catgattgcg atagaaaaga
    22141 ttgaaaaact gagtaaagaa aatttgggtc ttataaccct tgtcacagga gatgacattg
    22201 gtcagtatag ccagttgaaa tcccgcttaa tggagcagat tgcttttgat aaggatgatt
    22261 tggcctattc ttactttgat atgtctgagg ccgcttatca ggatgcagaa atggatctag
    22321 tgagcctacc cttctttgct gagcagaagg tggtcatttt tgaccatttg ttagatatca
    22381 cgaccaataa aaaaagtttc ttaaaagaaa aagacctaaa ggcctttgaa gcctatttag
    22441 aaaatccctt agagactact cgactaatta tctttgctcc aggtaaattg gatagtaaga
    22501 gacggcttgt taagcttttg aaacgtgatg cccttgtttt agaagccaac cctctgaaag
    22561 aagcagagct aagaacttat tttcaaaaat acagtcatca actgggtttg ggatttgaga
    22621 gtggcgcttt tgaacaactg cttttaaaat caaacgatga ttttagtcaa atcatgaaaa
    22681 acatggcttt tttaaaagcc tataaaaaaa cgggaaatat tagcctaact gatattgagc
    22741 aagccattcc taaaagttta caagataata tttttgactt aaccagactt gtcctaggag
    22801 gtaaagttga tgtggctaga gatttgattc atgatttacg cttatcaggg gaagatgata
    22861 ttaaattaat tgctattatg ttggggcaat ttcgcttata tttacagctt gccattcttg
    22921 ctaaagaagg aaaatcagaa cagcagcttg taaccagttt gtcagagata cttgatcggc
    22981 gggttaatcc ttaccaggtc aagtatgcgt taaaggattc taggacctta tctcttgcct
    23041 ttctaacagg agcggtgaaa accttgattg agacagatta ccagataaaa acaggacttt
    23101 atgagaagag ttatctagtt gatattgctc tcttaaaaat catgactcac tctcaaaaat
    23161 aggcgttgga ccgctttcca agagataatc aaattagttg aaagctctta ttttttgcga
    23221 taagatagac gtatcataaa ggaaaagagg acaggaaaat ggctattatt ttaccagaac
    23281 ttccatacgc gtatgatgct cttgaaccac agtttgatgc ggagacaatg actcttcatc
    23341 atgataaaca ccatgccact tatgtcgcta ataccaatgc ggctttagaa aaacacccag
    23401 agatcggaga aaatctggag gaactattgg cagatgtcac taagattcca gaagatattc
    23461 gtcaagcctt aattaataat ggtggcggac acctaaacca tgcgcttttc tgggaactat
    23521 tatcacctga aaaacaagac gtcactcctg atgttgcaca agccattgat gatgcttttg
    23581 ggtcatttga cgcctttaaa gaacaattta cagcagctgc tactggacgt tttggttcag
    23641 gttgggcatg gttagttgtt aataaagaag gacaactcga aatcacatca accgctaacc
    23701 aagatactcc aatttcagaa ggcaaaaaac caattctagc gcttgatgtg tgggagcatg
    23761 cttactactt gaattatcgt aatgtacgtc caaattatat caaagctttc tttgaaatca
    23821 ttaattggaa aaaagtatca gaactttacc aagccgctaa ataagaaaag ggtttccaat
    23881 ctcaaaaaga gattggtttt ttaagtggtt ttgtgaataa cagtgtgggg acatcatttt
    23941 ttagaaaaga taaaaatagt ttgtcttttt aatgtaattc cttgattttt agctaaattt
    24001 agctagaata gaatggatag gagattttag gtgttaagaa agaagaaaac taccattgta
    24061 gggattattt taggaatgat gatagtgacc ttacttgtcg gctgtttgac tggtaaaaag
    24121 aaaggctttc ctcctaaagc aggtgagcgg cagcaaacgg ctgacttaaa aatgaaaaaa
    24181 gattagaaaa actgttcaaa aaccgttacc tgaaaaatct aaagaggcta agcaagctgt
    24241 tatggatgca gatttagcgc aaatggatgc cttagggctc tattacgatg acaaccattt
    24301 gagtttggtc aatacggtca aggtgtattt aaaggaattt ggcattggtg aatctcaaat
    24361 tgccttttcc tacaaagacc tcaattcagg caggactgct gccatgaatg accatcagcc
    24421 aatgattgca ggaagcacct ataagctgcc attaaacatg ctggtggtag ataaggcaaa
    24481 ttatccatga cagagcgttt tgacattaca gaaactgatt atgaatatga tcaggaacat
    24541 catgcctatg ttgctcaatt caatggtgcc atgtctattc cggatatgca agaatattcc
    24601 ttggtgtact cagaaaacac gccagcttat gctctagcag aacgattggg tggcatgaat
    24661 aaggcttacc aattatttga ccgctacggg aaggtatccg gggctattac gaccattgat
    24721 cgtaatggca ataaaatcac caccgcttac tatttgcaag gtttggatta cttgtggcag
    24781 catcaggata aatataagga tatcttgtac tacattggtg aatctttccc tgatttgtat
    24841 tacaagactt atttgcctca tgtaaaggtt tatcaaaaac cgggttatgt cagagaagcc
    24901 ttaaatgtag gtgccattgt ttgcgaagag tctccttatt tgattactct ttattcatct
    24961 ggtttaggtg gagcaacaca agcatcagag gaagtcaatg gccttggtta tgtgcagctt
    25021 gttcagctaa catatgtaat taatgagtgg catagagtca atcacaacat gatctaatac
    25081 tcagttgaca ttagtctgat agtagacata cttactgatg aaagtcacta aaataagttt
    25141 ttgctcatta aaaaagcttg gcctgtttct tcaggtcaag ctttttgaga ttaatgctgt
    25201 gtttttgagg tcttattgtc acctcttgtt tcttgataat ggtgaagcaa ttttttgaat
    25261 tttttaacgg cagcgcgtgt tagctgctta gatgatttgc catttctgct gtaaatggga
    25321 ttgtgcttat ccctttgctg ttctaaatcg ggaactagaa aagttgcatc aggactatta
    25381 attgcttttt caataaaagc cggatcaaaa agcagaatag ggtcaatatc aagcagacgg
    25441 tgcaagtcat cgttttggtt gccataattg aaagaagaac cattgacaat actatttcgc
    25501 gttaggttat caggatctag tgctactaaa aattgtccag attctgacaa aataaactct
    25561 gagtgaaaat gaattaaaat cttatagttg gcttgaggaa tattgtctgt atagactgcc
    25621 tgatgattgc cattagcatc taaaatacta gcaaccttat tatggtatct agaagattca
    25681 taaagagcat aatcgtcatc ttctagagta gccaaatatt tggctaaggc atccgcatcg
    25741 gttttccctt tattcccata atgtgagcga acccactccg tttgctgggt tgatatgagg
    25801 taacggagtt gatgaatttt ccgattaaaa cggctataag gaatgtgttc gagattaaca
    25861 taataagcct gaatcagggt agataattgg tcccaaaaag gatctgttgg cgatagcttt
    25921 ggacttaatt gtgccaatac ttgctgtgtt tgttttgatt gtcctgtcaa ctcgtctggc
    25981 atctttaaat acgtcaagag cagttgtaac cgtttttgag cttcttgagg cgtctgtgcc
    26041 atctcttctt gttgccaaag cttttgaaag tcaggtgatc ctatcaggcg aatgcgacta
    26101 taaagttgtt cgaaatgata agagtttcct ctaacagcga gttgttcaag gtatcctttt
    26161 tggatggctt tataggacca tccgtcctta tcgagctttc gaacataatc aagcaagtcg
    26221 tggcgtaatt taatagcttc tgaaagctgg agttctcgta atttaggata gttttggtaa
    26281 aaaggtgcca ctaaattttg ataaagtttg tgttcttttt caaaggcaat ccgctgatag
    26341 tctttctgcc aaaaactgac gcaaaaagtc agaaacatca agaagaatag ccattttcca
    26401 agtaatttca taggttttta gttgacaaac atggcatcgc caaagctaaa gaaacgatac
    26461 ttttcgtcaa ccgcgtgtct gtaagcttca aggacaaagt cacgccctgc aaaggctgat
    26521 actagcatca caagggttga ttttggaaga tggaaattag ttgaaaaagc atcaactacc
    26581 ttgaattggt agcctggttt aataaaaata ttggtccaac ctgaatcagc ttgaatgtcc
    26641 ccttggaact tacctccgat ggtttccaac gtacgaatag aggtggttcc tacagcgaca
    26701 acacgaccgc cagcttgttt gacgtctctt agggtttgcg ctgcttcttc tgagagggaa
    26761 taaaattccg aatgcatatc atgctcatcg aggttatcca cggaaacagg tctaaaagtc
    26821 ccaagcccta catgtagggt caaataaacc aaatgtaccc ctttggcttc gatttttttg
    26881 agcaagtctg ttgtgaagtg gagaccagca gttggggcgg ccgcagagcc attttcttta
    26941 gcataaacgg tttggtatcg ctcagcatcc tctaattttt cgtgaatata aggaggcaat
    27001 ggcatttcgc cgaggctctc taaaacttct aagaaaatcc catcatagct gaattcaaca
    27061 attcgtccac catgttctaa ttcgtcgata atggtggctt tgagacgccc atctccaaaa
    27121 ttgacttggc taccaacctt gagccgtttg gctggcttgg ctaaaacttc ccattgatct
    27181 ccttgtgtat ttttaagcaa aagcaattcc acatgaccat gagtatcggg tttttctcca
    27241 taaagtctgg caggcaagac acgggtgtta ttcatgacga gggcatcccc tggatttaat
    27301 tgatcaatga tatggtcaaa atggctatca accatagttt tttgacggtg atcaataatc
    27361 aatagttttg aactgtctct tttttcgagt ggggtttggg caatcaattc ttctggtaac
    27421 tcaaaatcaa aatcgttagt attcattggg actcctttaa agccactagt aaatctcttc
    27481 ctagtaactc tttcatgtaa gataaatctt ttctattata tcacttgtcg tctaacccgt
    27541 catctttaat ctttcatcag aaagtaaagg ataggcttga caaaaattgg tatataccat
    27601 ataataaaaa cacgaaaggt ctataccaat tagaaaagga gaatgacatg aaaattattc
    27661 gggtccaaga tcagattgaa ggtgggaaaa tcgcttttac cttattgaaa gatagcttag
    27721 ctaagggagc taagacgtta ggattagcaa caggaagttc accaattagt ttttaccaag
    27781 aaatggtcaa aagtcctttg gatttttcgg acttaacaag tattaacttg gatgaatatg
    27841 tcggtctttc agtagagagc gatcagagtt atgattattt tatgcgtcaa aaccttttta
    27901 atgcgaagcc ttttaaaaag aattacttgc ccaatggttt agcaactgat atcgaagctg
    27961 aagctaaacg atatgaccaa atcattgcag ggcatccgat tgattttcaa gtgcttggga
    28021 ttgggcgtaa tgggcacatt ggttttaacg aacctgggac ctcttttgaa gaagaaacgc
    28081 atgtagtgga tcttcaggaa tcaactatca aagccaatag tcgtttcttt acaagtattg
    28141 aggatgtgcc aaaacaagct atctctatgg gaattgcctc catcatgaag tccaaaatga
    28201 ttgttcttct tgcctttgga caagaaaaag ctgatgcgat caagggaatg gtttttggac
    28261 caatcacaga agacctccca gcaagtattc tccaaaaaca tgatcatgtt atcgtcattg
    28321 tggatgaagc tgctgcgtca caattagatt aacctaacca gtcgttaaac cttgtaagaa
    28381 caaaaatgag gagatagtca acctcattta agaagaagtc ttgtaacttc tatttctttt
    28441 gcaagggtaa atttatgtta cactactaat atgcgtttag ataaattatt agaaggtaca
    28501 aaagttggct ctagaagtca agtgaagaaa ctgattaagg ctcaaggggt ttgggttgat
    28561 cacatgcctg ccagaaatgg tcgacaaaat gttgatcctg gcttgcaact gattgaggtg
    28621 actggacagc gagtgacgca tcctaaacat tcctacatta tcctcaacaa gccatcaggg
    28681 gtggtctcag ctaagaaaga tactaactat ttaaccgtta ttgatcagct tgctgaagaa
    28741 gataaaagtc cagacttgta tcctgttggc agattggata gagataccga agggttggtt
    28801 ttactcactg ataatggtcc cctagggttt cggatgttgc accctattca tcatgtttct
    28861 aaaacgtatc ttgtaaccgt taatggcttg ttagcagaag atgcctcaga tttttttgca
    28921 gctggtattt gtttcccaac aggtgaacaa tgtcaaccag ctcaattgac tattcttaaa
    28981 gccgacacag accagtcaca ggctagtttg accatttcag aaggaaaatt tcaccaagtg
    29041 aaaaaaatgt ttttgaccta tggtttgaaa gtgacctctc tgaaacggac gcactttgca
    29101 ggattggaac tcggtcattt agccagcgga gaatatcgtt atctaacagc gtcagaaagg
    29161 caactcatta aaacgtatct agactgagca aaacatgctt tcaaaaagaa gctattcttg
    29221 cttaatcaat aaagtgtcca ttgactatta tagtcatgat gagtccgact tttgctatgt
    29281 ttagtcatct tgttgttaca aaatcaggta agcaaaaaaa ttcaattccc actgtctcct
    29341 taacctgaag cgttataaaa acccttgtgt taaactctca taaattggaa atgggagtgt
    29401 tttttgatgg ctaaaaaaac taaagctact taaagtttgg tacttagttt tcgaataata
    29461 aggtggaaga tctaatagga ggtgacaaaa atattaaccg ctttagatgg taagaatcag
    29521 ctcatttcgc tagtaactca gcctatttca acgaaaccgc ctttttgttg cccagcttgc
    29581 aaatctcctg ttcgcttgcg tcaggggaca attagacgac ctcattttgc ccatgtacaa
    29641 ctagctcatt gtcaattcca agcagaaaat gaatctgagg aacatttgac gttgaaggca
    29701 aaactttaca ccagtttagt ccgaacagaa gctgtctgta ttgaaaaata tcttcctgag
    29761 ctgcaacaga tagcagacct ttgggtgaat gataagttag ccttagaaat tcagtgcagt
    29821 cccttgccag ttgagcgtct aaagaaacgc accaaagcct atcaagaaaa ggggtatccg
    29881 gtacgatggt tactgggaag aaaattgtgg cttaacactc acttaactgc tttgcaaaaa
    29941 cagtttcttt acttttcatc tagtttgggc tttcatttgt gggagctgga tgcagctgca
    30001 aatctgctac gtctcaaata tttaatccat gaagacttat ttggtaaggt aagttaccta
    30061 accaaaacca tttctttaga ccacaatatt atggaaatgt ttcgattacc ctatcagcaa
    30121 gaaatccttt attcttatca gaaaaaaatg actgtgaacc tatctaaaag aatacaaaga
    30181 gctcttttag ctagacaccc gaaatggcta cgacgacaag aaaaggctta cttatcaggc
    30241 tataatttac ttatgttaac aactgatgct ttttatcctc aatggagacc agttcaatct
    30301 tccagcggtt tttgtcaaat tgaaggaaat cttcgccctt attatgaaag ttttaaagtc
    30361 tattataaaa aagaaaagga taagaaggtg caaacgcttt tttcaccaaa atattatgtt
    30421 aaaatggata gtaatagaaa atgagaagga gatgttatga ctgataatcg cagccattta
    30481 gaagaaaaat acacttggga tttgagcact atttttgcaa cagataagga ttgggaagct
    30541 gaagtcagtg acttagctac tgaaattgac gcggctaagg atttttcagg tcatttacta
    30601 gattctagcg ctaatctgtt gacaattaca gatacttatt tagagttagc tcgtcgtgtt
    30661 gagaaagtct atgtttatgc tcacatgaaa aatgaccaag acactactgt tgccaagtat
    30721 caagaatacc aagctaaggc ttcaggcttg tatgcaaagt ttagcgaagt gttttcattc
    30781 tatgacccag aagttatggc attgagtcag gaagattacc aagcttttct tgcggaaaca
    30841 cctgatttaa aaaggtatca tcatttcttt gacaaattgt tccagacacg tgaccatgtg
    30901 ctcagccagg ctgaagagga attattagca ggagcccaag aaattttcaa cggcgctgaa
    30961 gaaaccttta gtattcttga taacgctgat attgtctttc cagtagttaa aaatgataag
    31021 ggggaagatg ttgaattaac ccatggtaac ttcattagcc taatggaatc taaggaccgt
    31081 agcgttcgcc aagctgctta tgaagccatg tatagtactt atgaacagtt ccaacatact
    31141 tacgctaaaa ccttgcaaac caatgtgaaa gtgcaaaact ataaggcgcg tgttcataaa
    31201 tacgattcag cacgtcaagc tgccatggct gctaacttta ttccagaagc tgtttacgac
    31261 actttattag aaacggttaa caaacattta ccactcttac accgttattt gaaattgcgt
    31321 caagaggttc ttggtttaga cgatttgaag atgtatgatg tttacacacc attatctgaa
    31381 acagatctag ccattggtta tgatgaggct ttggaaaagg ctgagaaggt tttagctgtt
    31441 tttggtaaag attacgcaga tcgtgtgcat cgtgctttta ccgaacgttg gattgatgtt
    31501 catgtcaata aagggaagcg ttctggagct tattcgggtg gttcctatga taccaatgcc
    31561 tttatgctat taaactggca agatacctta gacaatctat acactttagt tcatgaaact
    31621 gggcacagtt tgcattctac tttcactcgt gaaacacagc cttatgtgta tggagattac
    31681 agtattttct tggcagaaat tgcatccaca acgaatgaaa atattatgac agaagctctc
    31741 ttaaatgagg ttcaagatga gaaagaacgc tttgccattt taaaccatta cctcgatggt
    31801 ttccgtggaa cagttttccg tcaaacccaa tttgctgagt ttgaacatgc cattcatcaa
    31861 gctgatcaaa aaggagaagt gttaacaagt gaatacctta accagctata cgctgattta
    31921 aatgaaaaat attatggttt aagtaaaaaa gataatcatt ttattcagta tgaatgggca
    31981 cgtattcctc atttctatta caattattat gtttaccaat atgcaacagg ttttgcagct
    32041 gcaagctatt tagcggataa gattgtgcat ggcacacaag acgatatcga tcattacttg
    32101 gcctacctca agtcaggaaa ttctgattac ccacttgaag tgattgctaa agcaggcgtt
    32161 gatatgacaa aaggtgatta tttggaagcc gcctttaaag tctttgacga acgtctaaca
    32221 gaacttgaag tactcgtgtc caaaggcata cacaaaagct aaaatcacaa aagtcaagga
    32281 caaaaagagt ttaggattga tagaaatgga gattcacagt tggtgtgaat ctccatttct
    32341 atcggtcatt ttctttttat attttagtgg attatcagtc ctgaaaagca ggcttcattg
    32401 accttagttt gacggtttct tttttgtctt ttttattgtg tggtcactca cttaaataaa
    32461 actaatagtt gagtaaggcg cttcagatta acgctattct attgctttag agataacgtt
    32521 cgcataaaac atatttttat tatatttgac attttataaa agagataata gaattaaaat
    32581 actaacattt atgtttttta aaaaataaac gttcgtataa taaaaaagga gtttttcatg
    32641 gaaaaaacaa aacgttatat cattgcaaca gcagggatac ttctccactt aatgttggga
    32701 tcgacctatg cttggagtgt ttatcgtaat ccaattttac aagagaccgg ttgggaccaa
    32761 gctcctgtcg cttttgcctt ttctttagct attttttgtt taggtttatc tgctgctttt
    32821 atgggaaatt tagttgaaca atatggtcct cgtctaaccg gaacggtgtc agctattctt
    32881 tatgcttctg gaaatatgct gactggttta gcgattgatc gtaaagaaat ttggctttta
    32941 tatattggtt atggtgttat tggtggctta ggacttggag cgggatatat cacaccgata
    33001 tcaactatta taaaatggtt tccagataaa agaggcatgg caacagggtt tgctattatg
    33061 gggtttggtt ttgcgtcttt attaacaagt ccgattgccc agtggctaat tgaaacagaa
    33121 ggtttggtag caacatttta ccttttaggg ctgatttatc taatagttat gctttttgct
    33181 tctcaattga taataaaacc aactgctgct gagatagcta tactagataa aaaaagatta
    33241 caaaataaca gttatttaat cgaaggaatg accgctaaag aagcacttaa aacaaaatca
    33301 ttttattgtc tttgggtaat tttatttatc aatatcacat gtggcttagg acttatctca
    33361 gttgttgcgc caatggcaca agatctaact ggtatgagtc cagaaatgtc agcaattgtg
    33421 gttggtgcta tggggatttt taacggtttc ggtcgtttag tgtgggctag tttatcagac
    33481 tatattggac gccgtgtgac agtaatactt ttatttttgg taagcataat tatgactatt
    33541 agtttaatat ttgcacatag ttccctaatt ttcatgataa gcattgccac ccttatgact
    33601 tgttatggtg cgggtttttc tctcattccg ccttatttaa gtgatctttt tggggcaaag
    33661 gaactggcta cattacatgg gtatatattg acagcttggg caatagctgc attaactgga
    33721 cctatgttat tatctatcac tgttgaatgg actcataatt atcttcttac cttatgtgtt
    33781 tttattgtct tatacatttt aggattaatg gtagtattaa ggttgaagaa atgatgtcct
    33841 ataaaaaatt taaaagtaaa ccctagcaaa gcctagggtt tttttatata attaggttta
    33901 tggttaaatc atacagtaaa acagcaaatc ataatatgcg tcgtcctgtt gtcaaagaag
    33961 agcttgttca ttatatgcga acacggcaga agcaaaccac aggtttttta gcagagttag
    34021 agcaattcgc aagacaagaa aacattccta ttattcagcc tgaggtcgtg gcttatttca
    34081 ggtttttatt gcaaagctta cagccaaaac acattttgga gattggcaca gccattggtt
    34141 tttctgcgct tttaatggct gaaaatgcac cagatgcgac gattgtgact attgatcgta
    34201 atcgagagat gatcgatttt gctaaagcaa attttgctaa gtacgacagt cgccaacaga
    34261 tccgcctgtt agaaggagat gcagcagata tattatctac acttgaagga aattttgact
    34321 ttgtgtttat ggattccgcc aaatccaaat atattgtctt tctaccagag attttaagac
    34381 tgctaaaagt cggtggtgta gtcattttgg atgatgtctt tcaaggaggt gacattacaa
    34441 aaccgatcga agacattcga cgaggtcagc gtactattta tcgtggctta cagtctttgt
    34501 ttgatgccac tttaactcat ccaaacctaa caactagcct ggtccctctt agtgatggat
    34561 tattaatgat tcgtaaaaat caagcagata ttgtattacc agattaagta acattgataa
    34621 atttatgtta taataaatta gttaaagata aaaaggagct cagaagtaaa tgaaaaactc
    34681 aaataaactc attgctagtg ttgtgacatt ggcctcagtg atggctttag cagcttgtca
    34741 atcaactaat gacaatacta aggttatttc gatgaaaggt gatacaatta gcgttagtga
    34801 tttttacaat gaaacaaaaa acacagaagt atcgcaaaaa gcgatgctaa atctggtaat
    34861 tagtcgtgtt tttgaagctc aatatggtga taaggtttca aaaaaagaag ttgaaaaggc
    34921 gtatcataaa acagctgaac agtatggcgc ttcattctct gctgctttgg cacaatcaag
    34981 cttgacacct gagactttta agcgtcagat ccgctcttca aaattagtag aatatgcggt
    35041 taaagaagca gctaaaaaag aattgacaac acaagaatat aagaaagcat atgaatctta
    35101 tactccaaca atggcagtcg aaatgattac tttagataat gaagagacag ctaaatcagt
    35161 cttagaggaa ctaaaagccg aaggcgcaga ctttacagct attgctaaag aaaaaacaac
    35221 aacacctgag aaaaaagtga cctataaatt tgattcaggc gcgacaaatg taccgactga
    35281 tgtcgtaaaa gcggcttcaa gtttgaatga gggtggcata tcagacgtta tctcggtttt
    35341 agatccaact tcttatcaaa agaagtttta cattgttaag gtgactaaaa aagcagaaaa
    35401 aaaatcagat tggcaagaat ataagaaacg tttgaaagct atcattatag ctgaaaaatc
    35461 aaaagatatg aatttccaaa acaaggttat tgcaaatgca ttggataaag ctaatgtaaa
    35521 aattaaagac aaagcttttg ctaatatttt ggcgcaatat gcaaatcttg gtcaaaaaac
    35581 taaagctgca agtgaaagtt caacaaccag cgaatcatca aaagctgcag aagagaaccc
    35641 atcagaatca gagcaaacac agacatcatc agctgaagaa ccaactgaga ctgaggctca
    35701 gacgcaagag ccagctgcac aataagtagg attttattga ctaatcagtt aaaaaaactt
    35761 ataataattt agttaagaat tgtttatcaa ggattagata cagaaaagtg acggttgctg
    35821 cgagtcattg ataattagat aaacatgcta cttaacttta caacaattga tatcgtaaat
    35881 taagaatgac aagttcattg aatagaggtg gtaccgcggt tttcgccctc tgtgagatgg
    35941 acttgttttg tattgaggac tatttgaaat caacgaaagg aatagagtag actaggctgc
    36001 caggcttata gctgatctcc tagcaagaaa gacgagctgt gtcaactggt gtgattacat
    36061 tactagcttc agcataacac tgtttttctt actctttgta tctaaatttt tatgaaagaa
    36121 ttatcgtctg cacaaatccg ccaaatgtgg ttggatttct ggaaatctaa aggacattgc
    36181 gttgagcctt cagctaactt ggttcctgtg aatgacccaa cgcttctttg gatcaactca
    36241 ggtgttgcaa ccttgaaaaa atactttgat ggttcagtga ttccagaaaa tccacgtatt
    36301 accaatgcac aaaaatcaat tcgtactaat gatattgaaa atgttggtaa aacagcacgt
    36361 caccatacta tgtttgaaat gcttggtaac ttctcaattg gagactattt ccgtgatgaa
    36421 gctattgagt ggggatttga actcttgaca agtccagact ggtttgattt ccctaaagat
    36481 aagctctaca tgacttatta cccagatgac aaggattcat ataaccgttg gattgcttgt
    36541 ggcgttgaac caagtcactt ggtgccgatc gaggataact tctgggaaat cggtgctggt
    36601 ccttcaggtc cagatacgga gattttcttc gatcgtggtg aagatttcga tccagaaaat
    36661 atcggacttc gcctcttggc tgaagatatc gaaaacgatc gttacatcga aatctggaac
    36721 atcgttctct cacaattcaa tgctgaccca gccgtaccac gttcagaata caaagaatta
    36781 ccaaacaaaa acattgatac aggtgctggt cttgaacgtc ttgcagctgt tatgcaaggg
    36841 gcaaaaacaa actttgaaac tgacctcttc atgccaatca tccgtgaagt agagaagttg
    36901 tcaggtaaaa cttataatcc agatggcgac aacatgagtt tcaaggttat cgctgaccac
    36961 atccgtgcgc tttcatttgc tatcggtgat ggtgcgcttc ctggaaatga aggtcgtggt
    37021 tacgttcttc gtcgtcttct ccgtcgtgct gttatgcatg gtcgtcgtct tggcatcaac
    37081 gaaactttcc tttacaaatt ggttccgact gttggacaaa tcatggaaag ctactaccca
    37141 gaagtgcttg aaaaacatga ttttatcgag aaaatcgtta aacgtgagga agaaacattt
    37201 gctcgtacta tcgatgcagg tagcggtcac ttagattcat tgcttgcgca gcttaaggct
    37261 gaaggtaagg atactcttga aggtaaagat atcttcaaac tttatgatac ttatggattc
    37321 ccggttgaat tgacagagga attggcagaa gatgcaggct acaagattga tcacgaaggc
    37381 tttaagtcag ccatgaaaga acaacaagac cgtgcgcgtg cagctgttgt taagggtggt
    37441 tcaatgggga tgcaaaatga aaccctagct ggtattgttg aagaatcacg attcgaatac
    37501 gacacatata gtcttgaatc aagtctttca gtcatcatcg ctgataatga acgtaccgaa
    37561 gctgtttcag aaggtcaagc tcttcttgtc tttgctcaaa caccattcta tgctgaaatg
    37621 ggtggacagg ttgctgacac aggtagaatc aaaaatgata agggtgacac agttgctgag
    37681 gttgttgatg ttcaaaaagc accaaatggt caacctctac acactgtaaa cgttttggca
    37741 tcactttcag ttggaacaaa ctacacactt gaaatcaaca aagagcgtcg tttggctgtt
    37801 gagaaaaacc acacagcaac tcacttgctc catgcagccc ttcacaatgt tatcggtgaa
    37861 cacgcaactc aggctggttc attgaacgaa gaagaattct tgcgctttga ttttactcac
    37921 tttgaagcag taagcaatga ggaacttcgt cacattgaac aagaagttaa tgagcaaatt
    37981 tggaacgctc ttacgatcac aacgactgaa actgacgttg aaaccgcaaa agagatggga
    38041 gcaatggcac tttttggtga gaaatatggt aaagtggttc gtgtggttca aattggtaat
    38101 tattctgttg aactttgtgg tggaactcac ttaaataatt cttcagaaat cggtctcttc
    38161 aagattgtca aagaagaagg tattggttca ggcactcgtc gtattattgc agttactggt
    38221 agacaagctt ttgaagctta tcgtaaccaa gaggatgccc taaaagagat cgctgctact
    38281 gtaaaagctc cgcaattgaa agatgcagca gctaaagtac aagctcttag cgactcactt
    38341 cgtgatcttc aaaaagaaaa tgcagagctt aaagaaaaag cagcagctgc agcagctggt
    38401 gatgtcttta aagatgttca agaagctaag ggcgtgcgct tcattgctag tcaagttgat
    38461 gttgcagatg caggggcact tcgtacattt gctgataact ggaaacaaaa agactactct
    38521 gatgtgcttg ttctcgtagc agctattggt gagaaggtta atgtccttgt tgcaagcaaa
    38581 actaaagatg tccacgctgg taacatgatc aaagaattgg caccaattgt agcaggtcgt
    38641 ggtggaggta aaccagacat ggctatggca ggtggtagcg atgcaagtaa aattgcagag
    38701 ctgctagcag cagttgctga aacagtgtaa atccataaaa accaagttat ttagaacttg
    38761 gttttttata tttgaaatga ctaccatcat aacctttttg actattatcg ttgtcaaaaa
    38821 gaaaaagata tgttacaatt atagtaacaa atagacatga ggaggtgtta gagaaaatga
    38881 cacatggtat caaacttaag aggcagattt gcttatgggc aagtatcctg actcttttca
    38941 ttttaagtta tcatcttatc agggtaatct ggtttggagt acccttgagt tatctggttt
    39001 tgggctttac aggactagcc ttgctgttga atactttttc ttattttaga aagtaagtta
    39061 taagttaaag aagaatgaag gagatggcaa tggaggttat tcttaaaaat cggctcaaag
    39121 aattacgagc cagagatgga attaatcaaa cggaaatggc taaactggca ggagtatcga
    39181 ggcagaccat cagtctcatt gagcgtaacg agtacacccc atcagttata attgccatga
    39241 aaattgcaaa ggtatttcag gaaccagttg aagaggtgtt tcgtctagtg gaggtcgagg
    39301 aatgaaaaag aagaagaata gttttttacg cttgttaaaa atgttattgc tatcaagttt
    39361 ggcgggaggg attataggtg gcatggttgg agccttttta ggttaccatg gagagagatt
    39421 agatcagctc acgttcctaa aagatgatgt gattaacctg attatcttat taaatcgtct
    39481 agtagttgtc acaggcctca cactttcctt tgttttttta acgcaactga aaaaagagac
    39541 agctgtttat aacacaattg aagaggatga ttattctgaa aatggttacc gtcagctgaa
    39601 taaaaagcat gcttatacca tgttattgat tgccgtagct tctattttat ccatgtgcaa
    39661 tgtgctactt ggtctgacct taactaatga ttcacagcat gccatgctgg ccattccgct
    39721 tctggatatc cttttattat taatggtcat tccttttcaa gctttagcca tgaaacgtta
    39781 caatgccatt cggggaaccg atgttcctta ttttccaaat ttgaaagaat taaaacataa
    39841 tatcatggct ctagatgagg cagagttaca agcctatcat aagacatcgt ttgaaagtgt
    39901 gttgtcatta aatggagtca tcatcccttc cttgtatgtg attttatttt ttgtctatct
    39961 attcactggt caagtggagt taacagccat cttagttttg gtgttaattc agctctattt
    40021 actcgttaag agtgcaacaa tgaccagaca attttaccgt tagcctaaaa ggagactgtt
    40081 tatgaaagga ttcattaatt atttaaaaat agcagtgctc attattctgg ctatggtttt
    40141 caatgtcctt ccgatgattt tattgcaaaa gcaacacgat attcctatgg tgcttaattg
    40201 gggaattggt attttctact tggttattgt tggaagtgtc cttattgtat tatggcgtct
    40261 ttatcaagct aagcaagaca cccctattaa acagcaaaaa atgagattgg ttgactgggg
    40321 ttatttagca ttattttggt taatcatccg tgtgatagct attgcaggta cccttgttaa
    40381 ccagctatgg tctggtcaac aagtgagtgc taatgatgct gcaatacata ccttagctag
    40441 acttatcaaa ggtggtttcc cgctttatac tgccctattt gtacttgtga tagcttttat
    40501 cgctcctatt atggaagaac tagtctttag aggatttcct atgattgatc tcttcaaagg
    40561 aaaatcactt aaggtggcag gtttagtgac ctctcttgtt tttgctttac cacatgccac
    40621 caatagtgtt gaatttatca tgtacagctg tatgggcatt tttctctttg ttgcctatca
    40681 aagacgagga aacttaaaag acgctatctt gttacatatt tttaataact tgattgaagt
    40741 cattttgtta atgtcaatag gcttaggagt catataataa aaagcttcga atggatgaac
    40801 tgcaccccaa aagttggaca aaaaaatcta acatttgggt tggaattgtc aagaaaaaat
    40861 agacataaag ttaagctaat gagtaacta
//
LOCUS       DAUGHY010000012        40827 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.012, whole genome shotgun sequence.
ACCESSION   DAUGHY010000012 DAUGHY010000000
VERSION     DAUGHY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40827
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.012"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..161)
                     /locus_tag="VH752_000590"
     CDS             complement(<1..161)
                     /locus_tag="VH752_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ8288963.1"
                     /translation="MLVLYVSSKLIKRRRMKFMYSALLGNPVEHSISDELFEYLNHNI
                     GVLDEYKHK"
     gene            complement(230..301)
                     /gene="shp3"
                     /locus_tag="VH752_000591"
     CDS             complement(230..301)
                     /gene="shp3"
                     /locus_tag="VH752_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HEQ8288964.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
     gene            381..1232
                     /gene="rgg3"
                     /locus_tag="VH752_000592"
     CDS             381..1232
                     /gene="rgg3"
                     /locus_tag="VH752_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HEQ8288965.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINCFILSINKEEFSNCSYLIDEIK
                     TLLDNELNFYEQTVFLYATGYFEFKRQLETGVEKMKQAIQVLDILNEDKLKLHYADHF
                     DKLVNKD"
     gene            complement(1485..5024)
                     /gene="smc"
                     /locus_tag="VH752_000593"
     CDS             complement(1485..5024)
                     /gene="smc"
                     /locus_tag="VH752_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEQ8288966.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFDKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIKLNQTQDNLDRLEDIIYELDTQLAPLEKQAKVAKQFLEL
                     DANRKQLQLDILVKDIDIAQERQTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKK
                     QVISQESDQTQTTLLELTKLIADLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDG
                     FQAEEKQRTEQLLHIDQQLCDVKQQLNELSNALERFSSDPDQLMETLREEFVLLMQKE
                     AALSNQLTALKAHLDKEKQARQHKAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEM
                     LLQNYQEGDKRVQELERDYQLKQERLFDLLDQKKGKEARKASLESIQKSHSQFYAGVR
                     AVLQSQKKLGGIIGAVSEHLSFDSDYQTALEIALGANSQHIIVTDEAAAKRAIAYLKK
                     NRQGRATFLPLTTIKARSLSEHYHRQLATCEGYLGTAESLVRYDDSLSAIIQNLLSST
                     AIFETIDQANIAARLLGYKVRIVTLDGTELRPGGSFSGGTNRQSNTTFIKPELEQISE
                     ELTRLVEQLKITEKEVAALQSDLIAKKEELTQLKLAGDQARLAEQRAQMAYQQLQEKQ
                     EDSKALLAALDQSQTTHSDESLLAEQARIEEALTAIAKKKNALTCDIDDIKENKDLIR
                     QKTQNIHQALSQARLQERDLLNEKKFEQANQSRLRTQLKQCQQNILKLESILNNNVSQ
                     DSIQRLPQWQKQLQDATEHKSGAQKRLVQLRFEIEDYEARLEETAEKITKESEKNDTF
                     IRRQTKLETHLEQVANRLRAYAKSLSEDFQMTLADAKEVTNSIDHLESAKEKLHHLQK
                     TIRALGPINSDAIIQYEEVHERLTFLTSQKTDLTKAKNLLLETINSMDSEVKARFKVT
                     FEAIQKSFKETFTQMFGGGSADLVLTETDLLSAGIEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDFLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGITMQESGVSKIVSVKLKEAQEMTN"
     gene            complement(5025..5717)
                     /locus_tag="VH752_000594"
     CDS             complement(5025..5717)
                     /locus_tag="VH752_000594"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEQ8288967.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKD
                     YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            complement(6149..6958)
                     /locus_tag="VH752_000595"
     CDS             complement(6149..6958)
                     /locus_tag="VH752_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ8288968.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            complement(6962..8314)
                     /gene="vicK"
                     /locus_tag="VH752_000596"
     CDS             complement(6962..8314)
                     /gene="vicK"
                     /locus_tag="VH752_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEQ8288969.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADIELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTGTGKVYEIVRDYPITSVWLEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            complement(8307..9017)
                     /locus_tag="VH752_000597"
     CDS             complement(8307..9017)
                     /locus_tag="VH752_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8288970.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
     gene            complement(9179..10213)
                     /locus_tag="VH752_000598"
     CDS             complement(9179..10213)
                     /locus_tag="VH752_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ8288971.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PMIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDLVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHMFWKELASLVDQAFPGKTLSQAGELEKRLHQFRYVISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFILDSTGFFLNEVDAEVVTEKGIVNGASFNYGTDGPRHWDLDVDPISRHDP
                     QFRRDTLKGFRSPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
     gene            complement(10269..11516)
                     /locus_tag="VH752_000599"
     CDS             complement(10269..11516)
                     /locus_tag="VH752_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEQ8288972.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDGELLEKADFAVL
                     SSGTTGKAKLFWRCLSTWTRLFDYQNKMFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQAKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPAGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     ALNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCL
                     PLKDNGKINREVLLNKIPKQWLS"
     gene            complement(11473..12621)
                     /locus_tag="VH752_000600"
     CDS             complement(11473..12621)
                     /locus_tag="VH752_000600"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEQ8288973.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNAVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
                     KEYLNHWILRSHQKASYCQEQALLADLILDLPGASDQGIRPRLSSKVLSKVPPILGED
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGHYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK"
     gene            complement(13016..13165)
                     /locus_tag="VH752_000601"
     CDS             complement(13016..13165)
                     /locus_tag="VH752_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HEQ8288974.1"
                     /translation="MQLLDNVYGTLRSFAFMRPVKSSNDTSLADALQADTDMAFILVR
                     GINYQ"
     gene            complement(13251..14036)
                     /locus_tag="VH752_000602"
     CDS             complement(13251..14036)
                     /locus_tag="VH752_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8288975.1"
                     /translation="MAKLRRMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFVIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLDAYESAGS"
     gene            complement(14038..14856)
                     /locus_tag="VH752_000603"
     CDS             complement(14038..14856)
                     /locus_tag="VH752_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEQ8288976.1"
                     /translation="MWSFWKRYRPFLSAGIQELITYRVNFFLYRIGDVMGAFVAYYLW
                     KAVFDSSKQSLINGFTLSDMTFYIIMSFVTTLLTKSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFMEIGFRWIVLMSVGFPFLVVLSGIKVMAGLSILQVLASSCLYLVSLLLA
                     FLINFYFNICFGFSAFVFKNLWGSNLLKNALVAFMSGSLIPLAFFPKMVSIVLSFLPF
                     SSLVYTPVMIVIGKYSLSQIMVALSLQIFWLLVMVVLSQVIWKKVQYHLTIQGG"
     gene            complement(14858..15850)
                     /locus_tag="VH752_000604"
     CDS             complement(14858..15850)
                     /locus_tag="VH752_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ8288977.1"
                     /translation="MVMIEVSHLQKNFSKTIKEPGLKGALKSFVHPQREIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGYCRINGKIPQDNRQDYVRDIGAV
                     FGQRTQLWWDLALQETYVVLKEIYDVPEKAFRKRMDFLNEVLDLNEFIKDPVRILSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIIMIDKGQEIFDGTVTQLKQSFGKMKSLSFELRPGQEQVVSQFMGLPDIT
                     VERHELSLDIQYDSSRYQTADIIQKTMADFAVRDLKMTDVDIEDIVRRFYRKEL"
     gene            complement(15991..17934)
                     /gene="thrS"
                     /locus_tag="VH752_000605"
     CDS             complement(15991..17934)
                     /gene="thrS"
                     /locus_tag="VH752_000605"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEQ8288978.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTE
                     SVEEFVENILADIARKSRPDAQA"
     gene            complement(18356..19690)
                     /locus_tag="VH752_000606"
     CDS             complement(18356..19690)
                     /locus_tag="VH752_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ8288979.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKEELEKEGHEVYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGNGPYLQDLKHLAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAVIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLS
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            complement(19692..20690)
                     /locus_tag="VH752_000607"
     CDS             complement(19692..20690)
                     /locus_tag="VH752_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ8288980.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGIDLEQYGADSKKEAAFRRYFDIKEG
                     EKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGETNKWVIPAQVRQMVNGNHPKNL
                     IFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLEALASRQHLVLRDIPVYYGWVDQ
                     SSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQSRRLETVGHALVDVYKKVMEL"
     gene            complement(20821..21822)
                     /gene="ccpA"
                     /locus_tag="VH752_000608"
     CDS             complement(20821..21822)
                     /gene="ccpA"
                     /locus_tag="VH752_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEQ8288981.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPVVQYTRPNLSSISQPVYDLGAVSMRMLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            21996..23081
                     /locus_tag="VH752_000609"
     CDS             21996..23081
                     /locus_tag="VH752_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ8288982.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFRYVDSENPWEKIKAVLPNTAAKTIYAEFD
                     HLNVNKFHGLQTIFSGQFNNLTSYVQGMRLVKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHKMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLAAIDFIKPGVTAAQVDAAARQ
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMAGNDLVLEEGMCFSVEPGIYIPGKVGVR
                     IEDCGHVTKNGFEVFTHTPKELLYFEG"
     gene            23130..23795
                     /locus_tag="VH752_000610"
     CDS             23130..23795
                     /locus_tag="VH752_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ8288983.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SVGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEGDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            23806..24183
                     /locus_tag="VH752_000611"
     CDS             23806..24183
                     /locus_tag="VH752_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ8288984.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     ALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            24328..25254
                     /locus_tag="VH752_000612"
     CDS             24328..25254
                     /locus_tag="VH752_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ8288985.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMSNQLAFEYIFIDD
                     GSKDNTLGILRELAARFPNVHYLSFSRHFGKEAGLLAGLKEAKGNYITVMDVDLQDPP
                     ELLPVMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKYGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFGDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLETKKRPLYIIKEKH"
     gene            25465..26148
                     /locus_tag="VH752_000613"
     CDS             25465..26148
                     /locus_tag="VH752_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEQ8288986.1"
                     /translation="MEWIKLIGIVIIVLGFILKCDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQALTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYDGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            26148..27074
                     /locus_tag="VH752_000614"
     CDS             26148..27074
                     /locus_tag="VH752_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEQ8288987.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPAFNEQKAEESAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            27124..27771
                     /gene="pcp"
                     /locus_tag="VH752_000615"
     CDS             27124..27771
                     /gene="pcp"
                     /locus_tag="VH752_000615"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEQ8288988.1"
                     /translation="MKILVTGFDPFGGEAINPALEAIKKLPATIHGAEIKYIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(27886..28605)
                     /locus_tag="VH752_000616"
     CDS             complement(27886..28605)
                     /locus_tag="VH752_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEQ8288989.1"
                     /translation="MSIMITKGQVCLLRTYSYDSNLYTQGLEQLNNNHILLSAGRYGF
                     SKVGVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKATCNCLGA
                     YPFEGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELE
                     YVDGYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHL
                     DQQRFLITGKLYPLMLEVVLD"
     gene            complement(28611..29078)
                     /gene="smpB"
                     /locus_tag="VH752_000617"
     CDS             complement(28611..29078)
                     /gene="smpB"
                     /locus_tag="VH752_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEQ8288990.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGSG
                     MTLAPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR"
     gene            complement(29081..31414)
                     /gene="rnr"
                     /locus_tag="VH752_000618"
     CDS             complement(29081..31414)
                     /gene="rnr"
                     /locus_tag="VH752_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEQ8288991.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNDVG
                     YAIDGDTVEVVVKKPADRLKGTAAEAKVVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSALDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNVDRLTQSALMEINSQGHVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVALHRILEAMRSKRGALNFDTQEAKVIVNDKGIPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKAEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYSQPSQEKRDHFTQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVASVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGIK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            complement(31508..31744)
                     /gene="secG"
                     /locus_tag="VH752_000619"
     CDS             complement(31508..31744)
                     /gene="secG"
                     /locus_tag="VH752_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEQ8288992.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            complement(31790..31936)
                     /gene="rpmG"
                     /locus_tag="VH752_000620"
     CDS             complement(31790..31936)
                     /gene="rpmG"
                     /locus_tag="VH752_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ8288993.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            complement(31933..33126)
                     /locus_tag="VH752_000621"
     CDS             complement(31933..33126)
                     /locus_tag="VH752_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEQ8288994.1"
                     /translation="MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEY
                     YAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLI
                     LRLLTGVFAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
                     IRQVFLLVGVILFLCSLMTAVYVKEEFKPIRRFEMIPTKVILKQVKSPQIMLGLFVTS
                     MIIQISAQSVTPILSLYIRHLGQTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGN
                     HRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGI
                     SRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIK
                     VKDIV"
     gene            complement(33248..34750)
                     /locus_tag="VH752_000622"
     CDS             complement(33248..34750)
                     /locus_tag="VH752_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEQ8288995.1"
                     /translation="MTTMTIAKGSQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQN
                     GQPLRTTISEMGPMMLSRLLNLNDTQTGVLNIVFKIADEKGWLLIDLKDLQAILKEVG
                     DHASDYSSHYGNIAKQSIGAIQRSLLTLEQEGAHQFFGEPALDVADLMQLDVASGYGA
                     INILSATKLFQSPTLYTTFLLWLLSEFYELLPEVGDLDKPKMVFFFDEAHLLFKDAPK
                     VFLEKVEQIVRLIRSKGVGIFFVTQNPLDLPETVLAQLGNRIQHALRAYTPKEQKAVR
                     VAADTFRQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSDI
                     ESQQLVQSSPFASKYSQSIDRDSAYEKLAAKVLEDNRLAQEAIATAQREKEAKEAIKA
                     QAATKKANRRSVGRPRKTVVEKATDAFISTTVRTIGRELVRGLLGSLRKR"
     gene            complement(34940..35533)
                     /locus_tag="VH752_000623"
     CDS             complement(34940..35533)
                     /locus_tag="VH752_000623"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEQ8288996.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKADGELDRTKLSEMLFSNPNNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPLLMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNNGDMETLIKQVQSALLSLANPR"
     gene            complement(35530..36357)
                     /gene="mutM"
                     /locus_tag="VH752_000624"
     CDS             complement(35530..36357)
                     /gene="mutM"
                     /locus_tag="VH752_000624"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEQ8288997.1"
                     /translation="MPELPEVETVRRGLETLVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLIAKSQLSAFFAKRKLGPEPKKETFKLKTFEAALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPETASSRLNKAEIKRLHDETIRILALGIEKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKPCPRCGQAIVKLKVGGRGTHICPKCQKKRP"
     gene            complement(36529..37395)
                     /gene="rgg2"
                     /locus_tag="VH752_000625"
     CDS             complement(36529..37395)
                     /gene="rgg2"
                     /locus_tag="VH752_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEQ8288998.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLADEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGYYKLKQEEMSGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLG
                     GKGDEDWSEADL"
     gene            37484..37552
                     /gene="shp2"
                     /locus_tag="VH752_000626"
     CDS             37484..37552
                     /gene="shp2"
                     /locus_tag="VH752_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEQ8288999.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            38466..38735
                     /gene="stcA"
                     /locus_tag="VH752_000627"
     CDS             38466..38735
                     /gene="stcA"
                     /locus_tag="VH752_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HEQ8289000.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTTMTIGHS
                     KQNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(39083..39481)
                     /locus_tag="VH752_000628"
     CDS             complement(39083..39481)
                     /locus_tag="VH752_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEQ8289001.1"
                     /translation="MHRLLNRLIPEEDNNKLSRAAKEVVTEPRLNNPFLLPKKVLSLE
                     DDAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLAHHLLHSDGVIPSLNDKSA"
     gene            complement(39574..40644)
                     /locus_tag="VH752_000629"
     CDS             complement(39574..40644)
                     /locus_tag="VH752_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289002.1"
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGV
                     GYKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSGKSLTAQTV
                     PGMGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTV
                     FLVQPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGAFPDNIK
                     VSINPKGRQATITYGDGSTDIIPPAVLWKKGSVKEPTEADQSVGTPTPGIPGKFKRDQ
                     SLNEHEAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVG
                     SWLEPATALPSVEMSAEDRLKS"
BASE COUNT        12302 a         8681 c         7161 g        12683 t
ORIGIN      
        1 tttttatgtt tatattcatc cagaactcct atgttgtgat ttagatattc aaataattca
       61 tctgatatag aatgttcaac tggattacca agcaatgcag aatacataaa tttcatcctc
      121 ctacgtttaa ttagtttact actaacataa agtacaagca ttacacagag ctaaccatta
      181 cctataagtc tttagagtat ctaaagactt aggtatacac ctaagtaact tacccaccaa
      241 caataatgat aatatccatt gctaaaatta aaatcggcaa aaattttgaa attttcttca
      301 taagtggact tcctttcagt ttttatttat tgtacaataa aaaaaaacgg aattgatttt
      361 tgttgggaaa gtgggaaaat atgaaatcaa aacttggtgc cacacttaga aaagttcgaa
      421 aaggaaagca aattagtctg tgcttcgtgg cagatgaaca cctttcaaaa tcacaaatct
      481 ctcgttttga gcgcggggag tcagagatat cgtgtatccg actgatcaat attctagaca
      541 aattacacat tactttggat gagttcctta ttttacatga tgaagactat accaattccg
      601 aaacatttgc taacctggta cagtatattc gtaagcaata ttcatcacaa aatatcaata
      661 atatagcatg tttactttcc gacacttctg attacactct gaattctttt gagaaaacaa
      721 tggtaaagtc cattttacac acaatggatt caagcatcat tccatcagat gatgaattac
      781 ttcaactaac agattatctc tttaaagttg aaaaatgggg ctactatgaa atccttttat
      841 tgggtaactg tgtacgaaca attaactaca actcctactt cctattgaca aaggagatgt
      901 taaataatta catttactct tcacggaaca aaactaataa acgaattgtc actcagcttg
      961 ctataaattg tttcattctt agcatcaata aggaggaatt ttctaattgc tcctatttaa
     1021 ttgatgagat aaaaaccttg ttagataatg aattaaactt ctatgaacag acagtttttc
     1081 tctatgcaac aggttacttt gaatttaagc gccaattaga aactggagtt gaaaagatga
     1141 aacaagccat acaagttctt gatattttaa atgaagacaa gttaaaatta cattacgctg
     1201 accattttga taaactagtc aacaaagatt aagtgaaatc attctttacc ttttaacgaa
     1261 atgccaatca agagtgtctc aataaaaaat catctactct gacaactttt ctcttttacc
     1321 tatcgacagc taaaaagcct tggaaactgc tatttccaag gctttctttg tactctattg
     1381 tatcaattag cttattccga tttctgttgc tattagttta atcatgaaat ctcctaactt
     1441 atggccgaac atccaaaaaa acagaaggaa ctgccttctg ttttttaatt ggtcatctct
     1501 tgagcttctt ttaattttac agatacaatt ttagagaccc ccgattcctg catggtaatt
     1561 ccataaatac tatctgctgc agccattgtt cctttacgat gggtcacgac aataaattga
     1621 ctatctttgt caaagcgatt gaggaaatcc ccaaagcgct taacatttgc ttcatcaaga
     1681 gccgcttcta cctcatctaa aatcacaaac ggaatcgttt taacacgtat aatagcaaac
     1741 aataaagcca gtgctgacaa agctttttct ccgcctgaca tcaagttaag agattggatt
     1801 ttcttaccag gaggctgcac agaaatctct ataccagcag acaagagatc tgtttccgtt
     1861 aaaaccaaat cagcagaacc cccaccaaac atctgagtaa aggtttcctt aaaacttttt
     1921 tgaattgcct caaaagtcac tttgaaacga gccttcactt cgctatccat actattaata
     1981 gtctctagca aaagattttt agcttttgtc agatctgtct tttgacttgt taaaaaagtc
     2041 aaacgctcat gcacttcctc atactgaata atagcatcac tattaattgg tcctagtgca
     2101 cgaatagtct tttgtaaatg gtgtaatttt tctttggcac tttctaagtg atcaatacta
     2161 tttgttacct cctttgcgtc tgctagtgtc atttgaaaat cttctgataa acttttagca
     2221 taggctcgta agcgattagc cacttgctct aaatgtgtct ctaacttggt ttgacgacga
     2281 ataaatgtgt catttttttc gctttctttg gttatttttt ctgctgtttc ttctaagcgg
     2341 gcctcatagt cctctatttc aaatcttaat tggactaaac gtttttgagc accagactta
     2401 tgctctgtgg catcttgtaa ctgtttttgc cactggggta gtctttgaat gctgtcttga
     2461 cttacgttgt tgtttagtat gctttcaagt ttgagaatat tttgctggca ttgcttcagc
     2521 tgagttctta gacgagattg attagcttgc tcaaatttct tttcattaag taaatctctt
     2581 tcttgcaaac gagcttgaga taaagcttgg tgaatgtttt gtgttttttg cctaatcaaa
     2641 tctttgtttt ctttgatatc atcaatatca caagttaagg catttttttt cttagcaata
     2701 gctgttaatg cttcttcaat acgagcttgt tctgccaata aagattcatc agaatgagtc
     2761 gtctgacttt gatcaagggc agctaacaaa gcctttgaat cttcttgttt ttcttgcaat
     2821 tgctgataag ccatttgagc tctttgttca gctaagcgtg cctggtcacc ggctaatttc
     2881 aattgtgtca gttcttcttt ttttgctatc aaatcgcttt gcaaggcagc tacttctttt
     2941 tcagttattt ttaattgctc aactaatcgc gttaattcct cactgatttg ttctaactct
     3001 ggtttgataa aagttgtatt gctttgtcta ttagttcctc ctgaaaaaga accacctggt
     3061 cgtaattctg ttccatcaag agtaacaatt cttaccttat atcctaataa gcgcgctgca
     3121 atattagctt ggtcaatagt ttcaaagata gcagtactag ataaaaggtt ttggataata
     3181 gctgaaagac tatcatcata gcgaaccaat gattctgcag ttcccagata accttcacaa
     3241 gtcgctaatt gacgatgata gtgttctgat aatgatctag cctttatagt agttaaaggt
     3301 aaaaaagttg ctcgtccttg gcgatttttt ttcaaataag caattgctct ttttgctgca
     3361 gcttcgtctg ttacaataat atgctgacta ttagcaccta atgcaatttc aagagctgtt
     3421 tggtaatcag aatcaaaaga aaggtgttca ctgactgcac ctatgatccc tccaagcttt
     3481 ttttgtgatt gaaggacagc tcgtactccc gcatagaatt gactatgact tttttgaata
     3541 gattctaagc tagccttacg agcttctttt cctttttttt gatccaacaa gtcaaatagt
     3601 ctttcttgct tcagttggta atctctttct aattcctgaa cgcgtttatc accttcttga
     3661 taattttgga gcaacatttc gacttgctct ttttgagctt tataatgagc ttgagctttt
     3721 tgagactcgt cattaagttg atcaagctta gtaacaagca gctgatattc ttgagcctta
     3781 tgttgacgag cctgtttttc tttgtctaaa tgggctttta aagctgtcag ctgatttgat
     3841 aaggccgcct ctttttgcat caataataca aactcttctc ttagagtttc cattaattgg
     3901 tcgggatcac ttgaaaaacg ctctaatgca ttgctcaatt cgtttaactg ttgtttgaca
     3961 tcacaaagtt gttgatcaat atgcaataat tgttctgtac gctgcttttc ttctgcttga
     4021 aaaccatcca attgttcttg taactgttcg aggtgttttt tggcttctgc ctttttctct
     4081 gcttcttggc cactttctag cttaaccaat tcaatttgct tttccaagtc agctattagc
     4141 ttggttaatt ctaataatgt tgtttgggtt tgatctgact cttgactaat aacctgcttt
     4201 ttttgcttaa atttttggta atcttcttcc atagattgac gcttagcgta ataagatgct
     4261 aagtcttgtt gtaaagcagc taaagcttca gtatctttag tctgtctttc ttgagctata
     4321 tcaatatctt tgactagaat atctagttgt agttgcttac gattggcatc aagttctaaa
     4381 aattgtttag ctactttagc ttgcttttca agtggggcca actgcgtgtc tagctcataa
     4441 ataatatcct ctagacggtc taaattatct tgagtttgat ttaatttaat ctgagtttct
     4501 tttttacgtg ttttatattt taaaacacca gctgcttctt caaaaatggc gcgcctttct
     4561 tcaggtttac tattgaaaat ttcctctaca cgaccttgag aaataatgga gaaagaatca
     4621 cgaccaagcc ctgtatccat aaacaaatca tgaatatcac ggagccttac cttgcgacca
     4681 tcaatcagat agtcactatc tccatttcgg taaatatgcc tttcgacacg gatttctttc
     4741 ttggcagttt ttataaaatg atcggaatta tcaagaacaa ccgctacctt agcatagttt
     4801 agaggattac ggttttgtgt cccagcaaaa atgacatctg gcatcttgcc cccacgcaga
     4861 tttttagcac ttgattctcc taaagcccaa cgtaaacttt ctgtaatatt acttttacct
     4921 gaaccatttg gtcctacgac tgcagttact cccttatcaa attcaatttt agtcttgtcc
     4981 gcaaaggact tgaacccttc caactcaatt tcttttaaaa acatctaaac ctcactaagt
     5041 tgggcaaggg catttttagc agcatcttgc tccgctaatt ttttagactt acctaaacct
     5101 ttactaagga cagcaccatt aacaactatc gatacctcaa attgtttagc gtgggcaggt
     5161 cctttctcac taatgacttg ataatcaata gccacatcac cttttgtttg caaaaactct
     5221 tgaagacaag ttttatagtc ttttaccctt tcaaaattcc ctttttccac ctgaggaatc
     5281 atgacttgtt tcaagaagcg acgtactgca tcaatcccct tatctaataa aagagcacct
     5341 aaaaaggctt caaataagtc tcctaaaata gtatcacgac ggcgaccacc tgacttttcc
     5401 tcaccttttc ctagtttgat ataagcgtca aaagaacaaa atcgtgaaaa acctgccaag
     5461 ctttcctcac gtacaatcat ggaacgtaac ttagacatat ctccttctgt ctttttagga
     5521 tactttgcaa ataaatactc tgaaataatc aattgtagaa cagcgtctcc taaaaattcc
     5581 aagcgttcat tatgtgaaac gtttaggaga cgatgctcgt tagcgtaaga ggtgtgggta
     5641 aaagctgttt ctagtaaagt caaatcatta aattgaatat caaaagatgt tgagagtaac
     5701 tcttcaagct gtttcataaa aaatcccttt catatttgtc agtagacctt atttagtata
     5761 ccaaaaatcc acctagaaaa ccgactctaa gtggattttt cgtgatttat taaacatctt
     5821 aaatacccgt caaaggaaac tttctcatac aatcaaaaga ctctacacta tctgagggga
     5881 atgtcccact acagatagta tagagctatt ttgtttttag tctgaacaac taaaaaacta
     5941 ccttatcttt ataatcctca acacctttga taaatagatc agacaagcca aaactacagg
     6001 ctaatttaag tctattgatg ctctaaagga taaccaatga gagttcattg aactcagaag
     6061 acaatacgct ttaagacatc aaaaaatagc cctgactggt atatcaccaa tcagcgctac
     6121 tttgcatatt ctcttttagt ttcttatctc aaatatcagt taatggacaa gcagtatctg
     6181 gagaggtatc atggaccgta aagtctgtac ctactgctaa atctgccata gccagttgat
     6241 tgaccatcgt catatgcgct aactctttga tgttattttc tttactcaga tgacctaggt
     6301 atattttttt ggtattgtac cccaggcttc taatcatggc tcccgctcca tcttcatttg
     6361 ataaatgtcc taaatcagat aaaatacgct gcttcaagct ccaaggataa gagcctgatc
     6421 tcaaaatttc aatatcatga ttggattcaa tcaagtagcc atctgcattt tcaataatac
     6481 ctgtcatcct atcactgaca tatcctgtat ctgtcaacat cacaaaagac ttattatctt
     6541 tcataatccg ataaaattga ggatcaatag cgtcatggct gactccaaaa ctttcgatat
     6601 caacatctcc aaaggtcaaa actttatccc tttggaaaat atgtttttgt gaggcatcaa
     6661 gcttacctag catattgcac tcatccatca gctgccatgt tttttcatta gcatagatat
     6721 ccaaatggta tttgcgggcc ataacgccta cacccttaat atggtcagag tgctcatggg
     6781 taatcaaaat agcatccaaa tcctcaggtt tgcggtcaat ttcagcaaga agactggtaa
     6841 ttttcttacc agtaagccct gcatcaatca ataagcgttt tttaggtgtt tctaaataaa
     6901 aacaatttcc tgtggaccca gatgctaaaa tactatattt aaaaccactc tcattcattg
     6961 attagtctac atcttcctcc cattcttcat agatggctgc atctttttca taaggcaaga
     7021 caatagtaaa ggtcgatcct ttaccatagt cactcttagc ccagataaag ccatggtgct
     7081 gcttgatgat ttctttagca atggcaaggc ctagaccggt ccctccttgg gcacgacttc
     7141 ttgccttgtc tacacgatag aaccgatcaa aaataagagg caaatctgtt ttagggatac
     7201 ctagtccttg gtctgaaatg gaaataatta attgggtatc tgttgttttc atacggactg
     7261 tgatttttcc accatctgga gaatacttaa tggcattgtt caaaatattc tcgataacct
     7321 gtgtcatttt atcattatca atttcaagcc agacagaggt aatagggtaa tctcttacaa
     7381 tttcatagac ttttcctgta cctgtatgct gatttttaac caaatcaaat ctgtttaaaa
     7441 tagaagttat aaaagcagta aaattagtca tctctactgc taattgggtt acttgattat
     7501 caatccgaga caggtttaaa agatctgaaa tcatacgcat catccgatta gtttcatcaa
     7561 gagaaacttt tatgaaactt ggagcaatat cttctttaag tgcaccttca tcaagagcct
     7621 ccaagtagga tttaaccgaa gttaaagggg tccttaattc atgacttaca ttagagacaa
     7681 aaagacggcg ttcacgttct tctttttctt gttctgtggt gtcatggagc accacaacca
     7741 agcccgaaat aaaaccactc tctctcctat tcaacgcaaa gcgcaagcgt aatgagacaa
     7801 actcacccat atcatttcgg ctattaacag ttaccactgg tgttttggat accaaatcac
     7861 ggtaggtata tgaagtatca ccttctaaca aatctgtaat gtttttcttt agtgcctctt
     7921 ctttacttaa atttaattgc ttgcgagctg tctcgttaat catgatgatt ttaccagacc
     7981 ggtctgtagc aagtacacca tctgacatat aagccaaaat acttgccaag cgattttttt
     8041 cttgggcaag attttcatgc gtcaagcgaa aaacatctga caggtcattt aactggtctg
     8101 aaagctccat taactcaatg tctgcttttt cgtcgattat atcagtatag cgaccattaa
     8161 tcaagtctcg gattttatgg ctcatcatcc gaataatacg tgcatttcgg taatcacgca
     8221 ccgcaagatg gataaagtaa aaagcaacaa aaacaagtaa gataaggatc gctaattcaa
     8281 atgtggataa gtttccaatg atatctctag tcataagatt tcatgtagta cccaacacca
     8341 cgtcttgtta aaatatactc aggacgactt ggtgtgtctt caattttttc acggagacga
     8401 cgaacagtca catcaaccgt gcgcacatcg ccaaaataat catatcccca aacaatttcc
     8461 aataagtgtt ctcgtgtcat cacctgaccc atatggttag ctagatgatg caatagttca
     8521 aattcacgat gggtcaactc tacctcttga ccatgttttt tagcaacaaa cgcatctggt
     8581 aaaatctgta aattaccaat ggttagttcc tgtgtacctg aagaagcatt ttcttctgca
     8641 acagccgttt caatagtttc ggtacgacgc agatgagcct tgacacgtgc cagcaattcc
     8701 cgattagaaa agggcttggt cacgtaatca tcagccccaa tttcaagtcc aataaccttg
     8761 tcaaactcac tatctttagc cgacaacata ataatcggga catgactggt tttacgaatt
     8821 tccttggcta cttcaagacc gtccaactca gggagcatca aatcaagaat aattaaatct
     8881 ggcttttctt cttcaaaaat tgttaccgct tcgcgtccat caaaagctgt aacaatgtca
     8941 taaccttctt ttgtcaaatt aaacttaata atgtcagaaa tcggtttttc atcatccaca
     9001 ataagtattt ttttcatgtc tatacctcag aaagttcttg taattgatat tacaatttgt
     9061 gatttttgtg gttaataaaa aatcacaaaa ctatattttt ctaaattatt tctacctgat
     9121 tataccaaaa attcagccta attgacaatg acgactaccg ttacaaatag agtactttct
     9181 aaaaccaacc ctttatagga tgaatttgac gtttgaattg cttagcagat tttttgactc
     9241 tggcaaaact actcttgttg tggtaagcaa aaagtccttt ggcattaaaa taagagaaca
     9301 taaagtcatc tttttgagct ctaaaccact gacgaaatac tctttttggc gatcgaaatc
     9361 ctttaagggt gtcccttcta aactgaggat cgtgacggct aatcggatca acatctaaat
     9421 cccaatgtct aggaccatct gtgccatagt taaaagatgc accattgaca attccttttt
     9481 cggtcactac ttctgcatca acttcattaa gaaaaaagcc tgtgctatct agaataaatt
     9541 cggtatgaaa ccaaagaagt acttttatat tataggaaac ttcttgactt ggaaaaatga
     9601 ctttattatc ttcaaatctt agtttattat gtaacctggc agaatccatc aaggtataat
     9661 ctggtgcttg ccgccacaaa caaggccctt ttttagacct caaaaaaagg gccagagctt
     9721 gggcgtctgt catctcaatc attttataat ggtttctgat agattgagct tgttgactag
     9781 agatgacata acgaaattga tgcaaacgct tttcaagctc acctgcttgg cttagtgttt
     9841 tgccaggaaa agcttgatca actaagctag ccaactcttt ccaaaacata tgatctggca
     9901 atagagatgg atggaatcgg gtgagtagcg tttgagtttc atgcaactga ccacttagct
     9961 caaatggcat ctctaccaaa tcaagaagaa acgttagtaa ctgcttagct gattggtttt
    10021 ctttttcaat aggccatatt acccagaaag ttggagaacc aagtcgatta tcaatcatag
    10081 gctctttagc tatagcacga aatgcttggt ttgataaacc tgcgttttga tagcatttca
    10141 aggcctgata gcgatctaag atagcttgtt tcatggcctt ctcctcatct tttttgacca
    10201 tattataaca catctaaagc tgaatcgtca cttgtatctc actataagca aaacgaaacc
    10261 tgaagtggtt aactaagcca ctgtttgggt attttattta aaagaacttc tcgattaatt
    10321 ttaccattat cttttaaagg caaacaatca atcacaagat agtacttggg aagttgtcca
    10381 gagtttaaat aaagagctaa aaagtcttta ataggtgcta ggcaatcctt cttagtcaaa
    10441 actaatacta atattagggt ttcgttttct ccttttccta tctttaaagc gtgcgcttct
    10501 ttgacgttta gcgcttggtg agcgagttca actaggctag gcaggtggca tttcactcct
    10561 cgttgattaa cccagtcatc ttgtcgccct tctaaaatta aacccgctgg gctcattttt
    10621 cctaagtctg agacactgta tggttgtgac attccttcta cactataagg agtctctaca
    10681 aaaatttcct tatctttgaa actgatagac acatcaggaa agggctgtcc taccaaacca
    10741 ttaattttga cagcagctct gccattacac cacgttataa aagataattc actagccccg
    10801 tagaaaatga caatttcaag ctgaggaaat tttttgtaat aatgtcttag taattcttgt
    10861 gatatcattt gcgaagaggt caataagtga gtcgcagtca tgttgttctt agtcaaataa
    10921 ggtagcaagc gatttaggta ggtcggtaaa aggtaaaggt gactcacctt ttttgcttgc
    10981 cataagctta accacgtttt caaagacagc ttttggctta aaaccagaca tcccccggcc
    11041 catagttgag ctaatgctaa gttaagattg ccagtaaaac taaaactacc atgtaagaat
    11101 agacaactat taccagtcat accaaacatc ttattttgat aatcaaaaag tctcgtccaa
    11161 gttgatagac aacgccaaaa taattttgct ttgccagtcg ttcccgaact caaaacagca
    11221 aaatcagctt tttcaagaag ctcaccatcg acgtgtttta tttgctgctg aaatgtacct
    11281 gagatattag ggtgtaaaat gataggacag ctgccttctt ttaatcctcg taaaaaagag
    11341 agtaactgat taagatagcg actatgactg atgatatatg gtacactgtc ttttattgtc
    11401 tgatccttta ttaaaaccgc ttgccatagc tcttgataag tcaagcttat ctgatcggct
    11461 acgatagctt ttttatttgg gcattgcttt gcccaatatt ctagctttgt cagcatgact
    11521 aaactcctta tgatatttta gtaaaacagc aaacccttgg ccaccagctg ctgctatagc
    11581 agctattcca tagtgtccat ttttaatctc taaagccctc attaagtgca atattataat
    11641 ggctgctgag gcaccatagg gatgaccata agctaatgca ccaccaaata tattatagcg
    11701 gtccaataaa tctgggtaat gggtttcaaa tagaccatca ataacggcaa aggcttcgtt
    11761 ccattcaatc gctgtcatat ctgccatccc tagaccatgt ttttccaaaa ggacttgact
    11821 agccttaatc accattaaag ggctacgctg tgggtctcct gctacttcta ccacatcaat
    11881 cagtttaaaa gctgaaggct gactacttag ctgtaaaaag gcagcagcat catgggttag
    11941 gcaggcattg gctgcactaa tcacgtgatc ttctcctaag atagggggta cctttgacaa
    12001 caccttactt gacaagcgcg gtcgaatacc ttgatcactg gctcctggca ggtctagaat
    12061 aagatcagct aacagggctt gctcctggca ataactagcc ttttgatgac ttctcaatat
    12121 ccagtgattc aaatactcct ttgtaaagcc atgctctctt gccactcgtt ctgccccttc
    12181 aatcatcgca aaagggctga tactatctgg agaaaactga gctaccttgt aggctccttg
    12241 gcgccaatca gctgaagcat ataccgattc aggttgtaag gagctgcttt caatccctcc
    12301 cactaaaagg ttgcttgcca tacctgcctt gattttagca taacccacgg aaagagctgc
    12361 accagctgat gcacactgca tatcaaccgt caaagcagag acagattctc ctaaatgaga
    12421 atacagggtc atcaagcgcc caatattacc acccgttccg acggcattgc cacaaatcac
    12481 ttggtcaatg ggaactggat acttattttg taaagcattg atgagctttg ctccaaggat
    12541 ttctggttgt tctttagcaa attgtttccc tactaaaccg ataggggttc taaggcctgc
    12601 agcaatataa acatccgtca ttatattgac ctcctttatt ttcgatgtcc ttaaatggtt
    12661 attatataat tatttctgat ggttttcttt tttgaactaa taactgctaa aaatacacct
    12721 aaattaattt agagaatctc tttagtctcg ctagtgtgcc atcaataaaa tctataaaat
    12781 tctctaacgt cattgaaatg tcaagtccca caactattgt cgtagaagtc acttactttg
    12841 ttgctttaac attcaaactg taacctttgt ttttgataca gggtgacttt attatactgg
    12901 aaaaccattc ctaagaaaag aaaatcttat atagaataaa gaattagaca ccataaaaaa
    12961 gctaagctgt gacacttaac ttttttatgg tattgacaaa ccttgtcttt actatttatt
    13021 gatagttaat gcctctaact aaaataaaag ccatgtctgt atcagcctgc aaggcatctg
    13081 ctaagctggt atcgttacta gatttgacag gtctcataaa ggcaaaagat ctcaaagttc
    13141 cataaacgtt atctagcaat tgcatgcaac cacttgaaat atatctgctt atgttatttt
    13201 accgccctta atttagacca atctgctgat gatggttcca aacaatgcct ctaggaacct
    13261 gcactttcat aagcatctag gccccatttc caaagcttaa gcgacaaggc caaaaccagt
    13321 aacgacacca ctaccaagcc tccaatatta aagagcaggt gctgacctgt taaaaaataa
    13381 gaagcagggt aataagctgt aaaagcaaag ggaatgacaa aactgattaa ccaccgcaaa
    13441 aaggaatggt aaatggacat agggtacttg gaaaagtcat taaacatgta aaaaatatag
    13501 ataactgccc cagactgctt ggtccaaaaa gaaatgctgg cggtcgcaat ttttaaagag
    13561 gtgtaaatca aggtcgcaaa aggaataacc aaaataaaca aaagcacctt aggaagagtc
    13621 catacgatac taccagccgt ggtcactaat aagataaccc caaccaataa ttcacccagg
    13681 gcatccactt gaaatgtctc aaccaaaaca tgaaacaaag gactaattgg tctggtgagg
    13741 tacttgtcaa attctccttt gcggacaagc cgttgcccta aagcccaaag attgtcaaaa
    13801 aagaggtgat caattccctt tggaatcaaa gaaaagccat aaataaaggc aatttgttca
    13861 aacgtccagc cttctaaaga agggatatgt tggaaaagta ccgataaaaa gagcagatta
    13921 agaccctgag tgagaaaaac acctagcact cccaccacaa aatcaacctt gtattccata
    13981 atttgtttga ggtattgttt gataaaaata gcgtgcatac gtctcaattt tgccatctca
    14041 ccctccttga atcgttaaat ggtactgaac ctttttccag atgacttgcg ataagactac
    14101 catcaccagt aaccaaaaga tttgcaaact tagggcaacc attatctgac taaggctgta
    14161 tttcccaatg acaatcataa ctggcgtgta aaccaaagag gaaaacggta gaaaactaag
    14221 cactatagaa accattttag gaaaaaaagc caatggaatc aaactgcctg acataaaggc
    14281 aaccaaggca tttttcaata aattagaccc ccaaaggttt ttaaagacaa atgctgaaaa
    14341 gccaaaacaa atgttgaaat aaaaattgat taaaaaagct aataaaaggc taactaaata
    14401 cagacaactg ctggctagga cttgcaagat agacagcccc gccatgactt taataccaga
    14461 taagaccact aaaaaaggaa agccgacact cattagcaca atccagcgaa aaccaatttc
    14521 cataaagagg taagaggctg caaaatgaac tggtcgaagc aggcgcatga tgatagaacc
    14581 atctttcact tcttcgccaa tcataaagga gctgtctgat ttggtcagta aagtggtcac
    14641 aaaactcatg ataatgtaaa aggtcatgtc agacagagta aaaccattga ttaaggattg
    14701 cttagacgaa tcaaaaacag ccttccagag gtaataagcc acaaaagccc ccatgacatc
    14761 tccaatacga tacaaaaaga aattaacgcg ataggttatt aattcctgaa ttcctgcact
    14821 gagaaagggt ctataacgtt tccaaaatga ccacatctta aagctccttt cggtagaaac
    14881 gacgaacaat atcctcaata tcaacatcag tcatcttcaa atccctgacc gcaaaatcag
    14941 ccatggtttt ttgaataatg tccgctgttt gatagcgcga actgtcatat tgaatgtcta
    15001 aactaagctc atgccgttcc actgtgatgt caggtaagcc cataaactgt gagaccacct
    15061 gctcctgccc tggcctcaac tcaaaagaca gactcttcat ttttccaaag ctttgcttta
    15121 attgggtcac cgttccatca aagatttctt gccctttatc aatcatgata atccgatcac
    15181 agagctgttc aatatcactc aaatcatggg tcgtcagtaa aatagttgtc tcttcttctt
    15241 gattaatttg agtaatggca cgccgaatgt tgtccttcac agaaacatca agtccaatcg
    15301 ttggttcatc taaaaacaat actttaggat tatgcaacaa ggaagcagca atatcagctc
    15361 gcatccgctg accgagtgat aagatccgga caggatcctt aataaactca ttcaggtcca
    15421 aaacctcatt taaaaaatcc atgcgcttgc gaaaggcttt ttcaggcaca tcgtaaattt
    15481 cttttaaaac gacataggtc tcttgcaagg ccaaatccca ccatagttgt gttcgttgac
    15541 caaaaacagc tccaatgtct ctcacataat cttgacgatt atcttgtgga atctttccgt
    15601 taatgcgaca gtaacctgat gtaggcttta aaatacctgt taacatctta atcgttgttg
    15661 attttcctgc accattggct ccaataaaac ctaaaatttg ccctttgggt acctcaaaag
    15721 ataagtcctt aaccgcctca aagatttctc tttggggatg aacaaaggac ttcaaggctc
    15781 cctttaatcc tggctcttta atggttttgg aaaaattttt ttgaaggtgg gacacttcta
    15841 tcatgaccat ttaccattcc tcgtttctgt tatcgtctta tcaccgttac taaacaattt
    15901 attataacac catgaaaaag actgtgcaac cgtttgctta attttttaag aaagaaaaag
    15961 aagcccataa ggacctcttt ttgccattga ttaagcttgt gcatctggac gtgacttgcg
    16021 agcaatgtct gccaagatgt tttccacaaa ttcttccaca gattcagtgt gagtcgcctt
    16081 actaccatag cggcgaacat tgactgattt gtcttccatt tctttgtccc caacaattaa
    16141 ttggtaagga attttgctgg tttgagacgc acgaatcttg tactgcattt tttcattgcg
    16201 gtcatcgaca tctgcacgaa caccacggtc acgaagggtt tttgctactt cccaagcata
    16261 gtcaatgtgg gcttcatttg aaattggaat cacggttact tggtgaggtg ccaaccaagt
    16321 tgggaaagca cccttgtaag tttcaataag gatagcagtg aaacgttcca tagttgagat
    16381 aacaccacga tgaatcatga ctgggcggtg ttcttcgcca tctgcaccaa tgtatttaag
    16441 gtcaaaacgt tctggcaaca agaagtccaa ctgaatggtt gatagagttt cttcgttacc
    16501 aagagctgtt ttcacctgaa tatcaagctt aggcccataa aaagctgctt caccttctgc
    16561 ttcaaagtaa tctacaccca tctcatcaag tgcagcttta agcatacttt gagcattttc
    16621 ccacatctca tcattgtcat aatatttatg agtatcgtta gggtctcggt atgacaaacg
    16681 aaaacgataa tcagtcaagt tgaagtcagc ataaacatca atgattaatt gaagggcacg
    16741 ttgaaattct tcctggattt gctctggtgt tacgaaaatg tggccatcat tcaaggtcat
    16801 ttcacgtacc cgttgaagtc ctgatagggc acctgatttt tcgtaacggt gcatcatgcc
    16861 aagctcggca atacgaatag gcaattcacg ataagaacgc acatgatttt tatacacttg
    16921 aatgtggtgt gggcagttca ttggacgaag aacaaactcc tcaccatctc ccatatccat
    16981 tacagggaac atatcttctt gataatgatc ccaatgacct gatgtcttgt acaattctac
    17041 agaagcaagt ggtggtgtgt aaacgtgttg gtagcctgat gctaattctt tatctgtgat
    17101 gtagcgctca agtgtacgac gaatagtcgc tccatctggt aaccaaaatg gcaagccttg
    17161 accaacttct tgactaatca tgaacaagtc aagctcttta ccaagcttac ggtgatcacg
    17221 ttctttggct tcttccagac gagtcaagta agctttgagg tcttttttat caaaccaagc
    17281 tgttccatag atacgttgca tcatgttatt gtcgctgtta ccacgccagt aagcacccgc
    17341 cacattgagc aagtggaaga cttggatacg gcctgttgat ggcacatgag ggccacggca
    17401 aagatccaca aattcacctt gacggtaaac cgtcaagcca gcaccagcat gctcattaat
    17461 caattctacc ttgtaaggat catctttgaa caattccaaa gcttcttctt tagtcacttc
    17521 ttcacggata catgggtaat tttctgtcac gattttttgc atttcagctt cgatacgtgg
    17581 caaatcttcg tttgaaattt ggccttcagc attatctgta tcatagtaaa agccttctgc
    17641 aatggcaggg cccacaccca aatgaagatt tgggaacaag cgttttgcag cttgggcaaa
    17701 taaatgggct gcagagtggc gaagcacctc ataagcatct ttgtggtcag gtgtcacgat
    17761 ttcaatactg ccatcttctt caatggcacg agtggtgtca atgagttgat cattgaactt
    17821 accagctaaa gcttttttag ccaatgattt gctgatggac tcagcaatgt caaacgttgt
    17881 tacgccagat tcaaattcac gcacggcgcc atctggaaaa gtaatcttaa tcatttgctt
    17941 tctcctttaa gtcagttaag atttccacta aaacatggat agtaagacaa attagggaca
    18001 agaggttagt gaatagctca ataccaccac gtagaagaac aataccttat tcacgaatct
    18061 accctcccta gtccttaaaa acaaggctaa tcccctccaa aacaaaaaaa ctgaccgcat
    18121 cccaaaggac gcaatcagcg tggttccacc ttaatttatg caggacaaaa aacatgcctt
    18181 gcacctctag tggcgataag ggggccaccc ttttatgttt gcataaaaac caggaaggag
    18241 tatcagatta gctatccttg tgctctttcc accaaccagc actcgcttaa aagattctgt
    18301 ccaatctaac ttgtcttcaa gataattata gcaagttttc aaaaatttac aagtcttaat
    18361 ccagaaaatc tttgatagca tggaccagct tgataggagc cttgactact tttactgagg
    18421 tgatagctgt gaccttagca gcacgtttag gcaagtgaat agcaccttgc actaatttta
    18481 gagaactact tttaccagaa tgattaagat aaagactcag cttttgagct tctttgctat
    18541 ttctagcaat taacgtatct aaatagaacg tgtaaataga ttttccaaag tgctgtgctg
    18601 agatttcata acgttttttt gctaataacc gtttatccat aactggtgtt tttagtatgg
    18661 catcaataac agcatcagtt aaatctgttt cagcgtaata aagagtgcca aacattttat
    18721 cagtcaccac atcatctaaa taaggattgc catgagcaat aataggagtg ccactagcca
    18781 aactttcaat ataggtcaag ccctgagtct cactagttga tgctgagata aagaaatcac
    18841 aagccttata gtacagagca accttatcat gaggcaccat gcctgtaaag gtcacgtgtt
    18901 tgtcaacctc taactgcatc gccaagtgtt tcaaatcttg caaataggga ccatttccta
    18961 caataataag ctttatcttg gcattttcag ccaaaatagc tggcatctga ttgatgatag
    19021 cttgaatatt tttttcataa gaaatccgtg acaaactcaa taacatggtt tcatcaccag
    19081 caatgcccaa ttctgctttt aagttggtta cttcttctgc tgtgatgtca tcacgaatat
    19141 atttttccaa aggaatgcct gttgggataa cccgcttagg gatagtcact tcgtaacctt
    19201 ctagaagatt gaggacaatc ctacttgggc agataacccc atccaaatcc ttcaaatagc
    19261 ccctaagaag aggtttgacc atacttggtc gaatgatttt tccgttggca atataactca
    19321 cgtagtcctc atattgggta tggtaagtat ggacaacagg aattcgcaaa gctttgccta
    19381 tcattttccc taataagcct aagctaaact cagtttgcgt atgaataata tcaagattat
    19441 agtgttttgc aattttgtat gacgaaatga ggccacgata aaccacacgt ctatccgtaa
    19501 atgacacaaa aggaacactt ggcagtcgaa taatggtcgg gtcttcaaag cgtttgacat
    19561 ctctatcagt agtggtgaaa atataaactt cgtgaccttc tttttctagc tcttctttta
    19621 acgtacgaat actagtagcg actcctgaaa cttgtggaaa ataggtatct gtgaatagac
    19681 ctatacgcat cttataactc cattactttt ttatagacat ctactaaggc atggccaacc
    19741 gtttctaggc gacgactctg ggcaaccttg taaccagctt caactttgtt gctggcacca
    19801 gaaaagactt ttttcagagc ttctataaaa cctggtatat cggttgctaa ttccgcacta
    19861 ctttgatcaa cccatccgta gtaaactggt atatcacgta aaacaaggtg ctggcgactg
    19921 gccaaggctt ctaagacaac aatgccttct gtttcttcac gacttggaaa gaaaaaggca
    19981 tctgcaccag tcatggcacc ttcataaaca tcccctttaa tgtatcctgg gaaaataaga
    20041 tttttcgggt ggttaccatt gaccatttgg cgaacttgag caggaatgac ccatttgttg
    20101 gtctcgccaa accagataaa acgaacatct ggcatagctt gggcaacttt gacaaagtca
    20161 tcaattcctt tcctcagaaa aaataatcct gctcccataa ccactttttc accctcttta
    20221 atgtcaaagt agcgacgaaa agctgcttcc tttttagaat ctgctccgta ctgctccaaa
    20281 tcaatcccat ttgacactgc aaaaataggt gacttcactc cataactctc aatcaaagac
    20341 ttagaatata gggtaggggt aatgatagca tctgccttat tgtaaaagtg acaaaggtat
    20401 tttttaaacc aaggagataa tagatttgaa aaaataaaag aatttctaaa atcttcttct
    20461 gtagaatgcc catgcatgat aaccttctta ccagcttttt gtgcttttat catcagcagc
    20521 caacttttta aaccataggt attgagatga accaaatcat aagtttctct tggattagtc
    20581 gtaaaatgtt gaccaacaag tgacaaggct ttagcctgat gcttaatcgc tcgaccaatt
    20641 cctgattttc ttagataatt ttctgcttct aaatacaata agactttcat aagttctatt
    20701 atagctcatt ttatagtatt tttcactaag acatgcaaaa atcagcccta aggctgattt
    20761 tagtattgaa gaaacagtct attgtgatac tttttgttag atggtgctca taagtcacag
    20821 ttacttagtt gtcccacgtt ttttaatacc atgattcaaa agaatttctt tttcttccaa
    20881 ctcttctttg ttcatgattt tagtcaacat ccgcatgcta acagcaccta agtcataaac
    20941 aggttgactg atagaactca aattgggacg agtgtattga accactggtg aatcgttgct
    21001 ggtgatgatt tcaaagtcct ctggtacgcg tttacctgct tcaaacaagc cgtttaaaag
    21061 gcctgctgcc aattcatctt cagccacata agccgctgtt gctcctgagt taatgacgcg
    21121 ctgcgccaat tcaaatcctt ctttataaga atagttcgct tcaaaaacaa gaccttcttt
    21181 gaagtcaagc ttattgtgct tcaacccttc tttgtaccct gctaagcgca ctttaccatt
    21241 gatatcatca atgagtggtc ctgacacaaa agcgatacac ttatgatttt cagctaaaat
    21301 atcaacaacg tttgacaccg cagctctata gtcaatgttg acgcttggta attgatgatc
    21361 aagatctaca gttcctgcca aaacaactgg agtacgagag cgtgaaaatt cggcccggat
    21421 tttttctgtc aagtgatgcc ccataaaaat aataccatcc acttgcttgg caaaaagggt
    21481 attgacaaca ttaacctcct tgtcatcatc ttcatcactg gaggcaagca caatattata
    21541 tttgtacata gcggcgatat cgtcaatacc cttagctaag atagaaaaat aactatttgc
    21601 aatatttgga atcacaacgc caacggttgt tgtctttttg ctggcgagac cacgcgcgac
    21661 agcattggga cgatagtcaa ggcgatcaat aacttctaaa actttcttac gtgtattttc
    21721 cttaacattt ttattgccat taacaacacg actaacggtt gccattgaaa caccagcttc
    21781 acgggcaaca tcataaattg taatggtatc atctgtattc attttttatc ttcctttcta
    21841 tttgaaaatg acgctttcaa aaagtatttt agcatgattt gtaaccactt tcaagtgtta
    21901 taggttaatt tttgaaaaag ttttcaaaaa ttaactcgaa gcttgatttt ttaagctttt
    21961 tttgaaaaaa tagtaagtag aaagaaggtt agcctatgac aaaattagac caaatacgtt
    22021 tgtatcttga ccaaaaggga gctgaattgg cgattttctc agatcctgtt acgattaact
    22081 atttaactgg attcttctgt gacccacatg aaagacaact gttcttgttt gtctaccatg
    22141 atttagcccc tgtgcttttt gtacctgccc ttgaggtggc cagagctagc caggccatat
    22201 catttcctgt gtttcgttat gttgactctg aaaatccttg ggaaaagatc aaggccgttt
    22261 taccaaatac agcagccaaa accatttatg ctgaatttga ccaccttaac gtcaataagt
    22321 tccatggctt gcagactatc ttttcaggac aatttaacaa tctaacctct tatgtccaag
    22381 gcatgcgcct cgtcaaatca gctgatgaaa tcaataaaat gatgattgcc gggcagttcg
    22441 ctgataaggc tgttcaagtt ggttttgaca atatttcctt agatgctacc gaaacagacg
    22501 tcatcgctca aattgagttt gaaatgaaaa aacaaggcat tcataaaatg agctttgaca
    22561 ctatggtctt aactggaaat aatgcagcca atccccatgg cattcctgga acaaataaca
    22621 ttgaaaacaa tgccctactt ctctttgact taggtgttga gaccttaggc tacaccagtg
    22681 atatgaccag aactgtggct gttggtcagc cagaccaatt taaaatcgat atctacaatc
    22741 tctgcctaga ggcacaacta gcggctattg actttatcaa accaggtgta acagctgctc
    22801 aagtagatgc tgctgctcgt caagtgattg aaaaggctgg atatggtgag tacttcaacc
    22861 accgcttggg acatggtatc ggcatggatg tccacgaatt tccctccatc atggctggca
    22921 atgacttggt ccttgaagaa ggtatgtgct tctctgtgga accaggcatt tacattccag
    22981 gtaaagttgg tgtgcgcatc gaagactgtg gccatgtcac taaaaatggc tttgaagtct
    23041 ttacccatac tccaaaagag ttgctttatt ttgaaggcta agcccctacc atctggttaa
    23101 acaaaccctc gcgaaaggaa ggtattcgta tggatcaaac cattcatcac caaatacagc
    23161 aagcactaca ctttaggaca gccgttcgtg tttataagga agaaaagatt tctgatgagg
    23221 atttagccct tatccttgat gctgcttggt taagcccttc ttctgttggc ttagaaggct
    23281 ggcgctttgt cgttttagac aacaagccta ttaaagaaga aatcaagccc tttgcctggg
    23341 gggcccagta tcaactggaa acagctagtc actttattct tttaatagcc gaaaaacatg
    23401 caagatacga tagccctgct atcaaaaata gccttttacg acgtggtatc aaagaaggtg
    23461 atggtctcaa cagccgccta aaactctatg aatctttcca aaaagaggac atggatatgg
    23521 cagataatcc tcgggcactc tttgattgga cagctaaaca aacctatatc gctcttggta
    23581 atatgatgat gacagctgct cttttgggca ttgatacttg ccctattgaa ggctttcatt
    23641 atgataaggt caatcatatc ctagctaagc ataatgtgat tgatttagaa aaagaaggca
    23701 ttgctagcat gttatcccta ggctatcgtt tgcgagatcc caaacatgct caagttcgta
    23761 agcctaaaga agaagtgatt tcagtcgtta aataaggaga ttaaaatgaa agcattacat
    23821 acctgtatcc gcgtcaaaga ccttgaccag tcggtagctt tttacacaag cgccttccct
    23881 tttaaggaaa actatcgtaa agattttcca gatagtcaat ttactcttgt ttatctggct
    23941 ttagaaggag aaagttatga attagaatta acctacaatt atggtcatgg cgactacgac
    24001 ttaggaaatg gctatggtca tattgccctt ggctctgaac attttgaagc agaccataaa
    24061 aaacatcgac aagcaggttt tccagttacc gatatcaaag aactggctga caaatctgcc
    24121 cgctattatt tcattcagga tccagatggc tacaaaattg aagtcattga tttaaacaac
    24181 taatactatt aacaacagaa gctttaaagc ttctgttgtt ttatttttta acctgacgat
    24241 tgggataatc tcattattaa cagagttttg aaatatctca gcttttgtgt tacaattgcc
    24301 ctaaaattcc atttgaaaga aaagtctatg acactactat ctatcattgt cccctgcttt
    24361 aatgaagaag caaacattct cccttatttt gaagaaatgc atcaactaga aacgtccatg
    24421 tccaaccaac ttgcttttga atacattttt attgatgatg gttctaagga taacacctta
    24481 ggtattttgc gtgagctagc tgctcgcttt ccaaacgtgc attatctgtc tttttcacgc
    24541 cattttggca aggaagctgg actcttagct ggactaaaag aagccaaagg caattacatt
    24601 actgtgatgg atgtggatct gcaagatcca cccgaacttt tgccagttat gtacgctaag
    24661 ctaaaagaag gttatgacat cgttggcacc aggcgccaaa atagacaagg agaaccacta
    24721 attcgttcaa tgtgttcgaa tctattttat ggactgatca aacacttatc agatactgaa
    24781 atggtcaatg gagttcggga ttatcggtta atgaccagac aagtcgttga tagcattctt
    24841 gagttgggag aggttaatcg tttttcaaaa gggatctttt cgtgggttgg ttatcgtatc
    24901 acctatctta gttttgaaaa tcaaaaacgc aaatatggaa aaagtcgttg gcatttttgg
    24961 gagttgctcc gatattcttt agatggcttt atcaactttt cagaaatgcc gttaaccatt
    25021 gctacctgga cagggacttt cagctttttg atttctattt tcgccatctt atttatcatt
    25081 atcagaaaga tactctttgg tgatcctgtc tctggttggg ccagcactgt ctctattatt
    25141 ttattcatgg gaggcattca gctcttttgc atgggtatta ttggaaaata tatttcaaaa
    25201 atattccttg aaactaaaaa aaggcctctc tatattatca aagaaaaaca ttaaattaaa
    25261 acatttttct catcaatcgt ttcctactcc ttctgtgcgc gaagctttct ttttaagccc
    25321 gttataccaa atgataatga cctttccaaa atagtatcac cttccttaac actttaagta
    25381 gaaaaggaac tattaggttg agcctttttg caaatcctag cgactaatgt tataatcgtc
    25441 atactattga aaaaggagtt ctttatggag tggattaaat taatcggtat tgtgattatc
    25501 gtgttagggt ttattttaaa atgtgatgct atcgctaccg tagttgttgc aggacttgtt
    25561 accgcgctgg tttcaggaat ttcgtttatt gattttctag atattttagg aaaagaattt
    25621 accaatcaac gtttgctaac cattttcttc atcactttac cgcttatcgg cttgtcagaa
    25681 acttatggac tgaaacaccg agctactcag ttgattcaac gtgttcaagc ccttaccgtc
    25741 ggccgtttat tgactctcta ccttattatt cgagaattag caggtctctt ttcgattcga
    25801 ctagggggac atccacagtt cgttcgcccc ttgattcaac ctatgggaga agctgctgct
    25861 aaagccaaca ttggagaaga gttgaccgat gcagaaaaag atgatatcaa agctatggca
    25921 gctgccaatg aaaactttgg caatttcttt gcccaaaata cctttgttgg cgcaggaggt
    25981 gtcctactta tcgctggaac tttagaacag cttggctatg atggtaacca agctaaaatt
    26041 gcattttctt ctattttaat tgctattatc tctattatta tagttgctat ttacaattac
    26101 ctctttgaga aaaaaatgga acgtcaacat cagaaaggag ataactaatg gctaacattg
    26161 caaaccttgt tttagaaacg atatatatcc ttatcggctt gcaactgttt cacacagcct
    26221 actgcacctt taaagataaa accaaccctg tctattttgg gacagctctg ttctggggat
    26281 tgctgggtgt taccttcgtt ggcggtgcct ttttgcctaa taaagttatc ggttttatcg
    26341 tgattgtcct agctctttta accttgttta agcaagttcg tattggaaca ttaccagcct
    26401 ttaatgaaca aaaagctgaa gaaagtgctc accgcattgg taactggatt ttcttaccag
    26461 ttatgttaat ggctatgatt tctctacttc ttgccttgat ccttcctgat ttcagtaaaa
    26521 gtgccattgg gattgctggt atcttagcca ctattgctat tttaataatc actaagcaaa
    26581 aaccatctgc tcttttagct gaaaataatc ggatgaacca acaagtgtcc acaagcggta
    26641 ttttgccaca gctcttagga gctttaggag ctatttttgc agctgctggt gttggtgatg
    26701 tgattgcttc tctcattcgg gagattgtac ctgctgatag ccgcttcttt ggcgttctcg
    26761 cttatgtgct tggcatggtt atttttacta tgattatggg aaacgctttt gctgccttta
    26821 ccgttattac aaccggtatc ggtgttcctt ttgtttttgc tttaggagct gaccctatta
    26881 ttgccggtgc tttagctatg actgctggtt tttgtggtac cttattaaca ccaatggcag
    26941 caaactttaa tgccttgcct gttgctttga tggagatcaa agatcgaaat gcagttatca
    27001 aaaagcaagc tcctatcgcc cttgttttaa ttattagtca cattgcctta atgtatttac
    27061 ttgcttatta ttaacatttt tgctaagtta gtagcgtcag ttattcattg aaaggacatt
    27121 attatgaaaa ttcttgtaac aggctttgat ccctttggcg gcgaagctat taatcctgcc
    27181 cttgaagcta tcaagaaatt gccagcaacc attcatggag cagaaatcaa atatattgaa
    27241 gttccaacgg tttttcaaaa atctgccgat gtgctccagc agcatatcga aagctttcaa
    27301 cctgatgcag tcctttgtat tgggcaagct ggtggccgga ctggactaac gccagaacgc
    27361 gttgccatta atcaagacga tgctcgcatt cctgataacg aagggaatca gcctattgat
    27421 acacctattc gtgcagatgg taaagcagct tatttttcaa ccttgccaat caaagcgatg
    27481 gttgctgcca ttcatcaggc tgggcttcct gcttctgttt ctaatacagc tggtaccttt
    27541 gtttgcaatc atttgatgta tcaagccctt tacttagtgg ataaatattg tccaaatgcc
    27601 aaagctgggt ttatgcatat tccctttatg atggaacagg ttgttgataa acctaataca
    27661 gctgccatga acctcgatga tattacaaga ggaattgagg ctgctatttt tgccattgtc
    27721 gatttcaaag atcgttccga tttaaaacgt gtagggggcg ctactcactg actgtgacgc
    27781 tactaaacct attaaaaaaa cagagatatg aactaactct gttttttttg tgctaaaaat
    27841 gaaagaccta gggaaacttt tcataggtct ttctcaattg tcatcttaat ctaatactac
    27901 ttctaacatc agcgggtata gtttaccagt aattaagaaa cgttgttgat ctaaatgagc
    27961 aatcccattc aaaacattaa ggtcagggta atgggactta tcaagattta aggcttttaa
    28021 caaaggacta atatcatagg tggctaccac ctttccagaa tcaggttgga gtttgacaat
    28081 agtattggtt tgccaaatat tggcatagag ataaccatct acatactcta attcgttaag
    28141 cattgagatg gggacacttt ctatagcaac tagtacggta tccaataaag caaaatcttt
    28201 ggggtctcgc ttttgcaaaa aagcatttcc actagtcatc caaagacact ggttttcctt
    28261 atcataggct aatccccagc catctccttc aaatggatag gcccctaaac aattgcaggt
    28321 agccttatca aacttgtagg ctacaccctc tttataggtt agtaaccaaa aataatcctc
    28381 aacaactgtt agcccttctg caaaaactgt atctggaaaa gctatttttt ctgaaaagat
    28441 ctcttgagta aggtcataca cccctacttt tgagaaacca taacgaccgg cactaagtaa
    28501 tatatgattg ttgttgagtt gttctaatcc ctgcgtgtat aagttgctat cataagaata
    28561 agttcgtaac aaacaaacct gtcctttagt tatcataata ctcattcacc ttaccttgca
    28621 ttgtagtgtt tcatttgttt tttaatatct ctttcctgat cacggcgttt aatggtttcg
    28681 cgtttgtcat actcgtgttt ccctttagct agaccaatca gaactttagc aaagccatcc
    28741 ttgagataaa ctttgagagg agccaaagtc atgccagatc ctttaagttc atttgctaga
    28801 tgcgtaatct cacgtttttt gagcaacagt ttgcgggtcc gttcaggatc agcgttccaa
    28861 atattacctt gttcaaaagg tgcaatatgc acgttgacca accacgcttc cccattctta
    28921 atttgggcaa agccatcttt cagttgaata cgagctgcac gaacactttt aatctcagtt
    28981 cccgttaaga caatccctgc ttcaacggtt tcaacaatgt gataatcatg tctagccttt
    29041 ttattttgcg ccagtatatg accttcacct tttgccatag ttaactcctt ttacgactct
    29101 tcttttttgc ttgttctttg taaaatggtt ttttagaccc ttttttagtt ttacccttgg
    29161 tttttgcctt ggctacttct ttaggttctt ttttctttat ccctttagat gattttgagg
    29221 atttacggcg atctcgtcga ctagctttat ccgacatctt gattttttca accacatcaa
    29281 agtcgctagg taaatactca aagtcaatgt caccagtttc cttgtcagcc ttaaccaatt
    29341 tcacacgaat aggttgacca actttaaaga ctttacctga tttttcacct tgaaggctta
    29401 gcgttctttc attaaagtga tagtattccg gtaagctagt aatatgaacc aaaccttcaa
    29461 tcgtatttgg caattctaca aaaaaaccaa atttaacgac actggccacg ataccgtcaa
    29521 attcttcacc cacatactct gccatatact cagctttttt catggcttca accacgcgtt
    29581 cagcatcaat agcacgacgc tctaactgtg aagaagatgt tgctaattct ggaatgatct
    29641 gagtaaaatg gtcgcgcttt tcttgactag gttgactgta ctcacgaacc atgcggtgaa
    29701 ccaacaagtc agggtaacga cgaatcggac tggtaaagtg agtgtaatat tctgcggcca
    29761 aaccgtaatg cccatgatta tgttcagaat atcttgcctg ttgcatagag cgtaagagca
    29821 tcatgttaag cacttctgct cctggttgac cttcgacttt tgccataaag gcttgcaagg
    29881 cttcctgaga aatcttgttg gctgttcctt gaatgtgaat accaaaggtg ctagcgtaat
    29941 caataaattg ttgaagtttt tcggcctttg gttcttcgtg aatacggtag ataaacggta
    30001 acttagcttt tgcaaaatgt tctgccacac actcattggc tgctagcata aaggactcaa
    30061 tcatccgctc tgcaattccc ctttgacgaa gcaccacatc aaccggtatt cctttgtcat
    30121 taacaataac tttagcttct tgcgtatcaa aattcaaggc gcctcgtttt gaccgcatag
    30181 cttccaaaat cctatgcaaa gccaccatga gggtcacatc atcagcaatg ctagcgaatt
    30241 cttggagcgc ttcttcatct cctgcaatca tgtcattgac cgtactatac gtcattcggt
    30301 aagttgtctt aatcactgat tgacagattt gataattgac cacatgccct tgagaattaa
    30361 tttccatcaa ggctgactgg gtcaaacgat caacgttagg gttcaaagag cagatgccat
    30421 ttgacaaacg ctctggtaac atcggaacca cacgatcggt gacatagaca gaagtgccac
    30481 gggcgatagc ttccttatca agggcagatc cttctgtcac ataatatgag acatccgcaa
    30541 tatgtacccc aagttcatag ttaccgttat ccaatagctt aatatggata gcatcatcta
    30601 agtctttggc gtccgcacca tcaatagtaa tggtggtttc ttgacgcaaa tccacacggc
    30661 ctattaaatc tttagcagtt ggtgcttctg aaatagcatt ggcctcggct aaaacttctg
    30721 ctgggaactc tgacacgata tccatcgatt caaggacttc taggacatca attccaacgt
    30781 ctccttgatg gcctacgata tcacgcacac tggctacaaa ataatcgtgc ccgcgtgtcg
    30841 ggtatttatc aatatcaacc ttgatgattt cagtgccttt aagcacaaca ggttcttttt
    30901 tgatatagat tttttgttga atcttttgat ttttggacct gatataacca gcatattttg
    30961 gtttgtcatc atctaaaata aaggttccaa cagctgtttt taaagaacgg tcaacaatag
    31021 caacgacctt agcttcagcc gctgtgcctt tgagacggtc tgctggcttt ttaaccacta
    31081 cttcgacagt atcaccatca atagcatacc caacatcatt acgaccgata aacatgtcat
    31141 cttcattctc atccacatgt aagaaaccaa agcctgcttt attggcacga aaaacaccct
    31201 gaacagtagg ttcttttttc ttttcacgct cttttctaag agccagactg ccatcatctg
    31261 aaaaccgtaa caggctctga ctttccattt tggaaatggt tttaataagg ctgggaaaat
    31321 gcttagctcc agccatctcc aaggccgcag ccaagtcgtt cacattggac ttgccatgct
    31381 ccttgaggta agtcattatt ctttctttca tcatacctaa tttctatttc tgttttgttt
    31441 gttggaggat atcaatccgt ttcgctgata aaaaaatgag ctagactaac tctgtcaagc
    31501 tcactcctta tttacttgat aaaatggcaa gcacaagtgc aatcgctagc caaaaaaata
    31561 ctagaactgc tgtgaatcgt tgcatgaatg cttcaaagcc acgagctttt gtacgttcaa
    31621 acaaggcttc tgaaccgctg ctatcaaaaa cattgctact tgggtttttt tgaggttgca
    31681 taaaaatagc aatcacaagc aataccgaca gtactagcaa tatcgtaagt agtaagttgt
    31741 acatgttatt tcctccacta ttgtcatgac cattctaaca caattcttat caagtttcaa
    31801 catgcagggt cacttttctc tccttggggc agtatttggg aacttttatt ttttccggat
    31861 gactgctttt atttttactg gttagataat tgttactgcc acactcactg cattctaaat
    31921 taatttttac tctcacacaa tatccttaac tttaatatat tttcgaaata taatcaaact
    31981 ccatattaaa ttaacaaaca caatcaaact agtcacataa aaaacagaac ggtagcctag
    32041 cactacagcc acatttgatc cgataaaagg gccaatgact tgaccaaggt tactaaacat
    32101 ttgattatag gcaaatactc tagaaatacc ttcttttggt gttaacttag ttagtaaaga
    32161 gttaatactt ggcatcaaag cgcccacacc aaacccataa gcaaagcgta gtactcctaa
    32221 ttgaaaactg gtctgcgcta aggcactgct aaaatacatg ataaagctat aacacaaagc
    32281 ggctaacaat aaccgatgat tgccaaatcg atctcctaat tttcctaagt aagaactgct
    32341 aaataagcta gaaaacccca tggccgaaac taccaggcct gaagtaaaca ttaagttgtg
    32401 agtttgacca agatgacgaa tatacaatga taaaatgggg gtcactgatt gagctgaaat
    32461 ttgaataatc atactagtca caaacaaacc aagcataatc tggggacttt ttacctgttt
    32521 taaaatgact tttgtaggta tcatctcaaa gcgcctaatc ggcttaaact cttctttaac
    32581 ataaacagct gtcattaaag aacataaaaa taatatcaca ccaactaata agaaaacttg
    32641 tcgaattcct aatagctctg ccaaaattcc ccctagtaaa ggaccaatta aagcgcctgc
    32701 tgtcactcct gttgctaatg tgcctaaagc ataacctgac tcctcttttg gggcttggct
    32761 ggcaatcaag gcagtggcat ttggcacata gcccgcaaag acacctgtta ataatctcaa
    32821 aatgagtaac caaaacacat taggaataat agctaaacca cccattgtaa atgtcatgac
    32881 aaaacttgct cgcagcatca taggttttcg gccataacga tcagccaact tcccccaaac
    32941 aggagcaaat aaagctgaag caagtgccgt cacagcaacc gccagaccag catagtattc
    33001 tactaattct gtcggagtac ctaaattttc cacatacaga gccataaaag gcataacaag
    33061 tgaaaagctt gccccagtaa agaagttacc aagccaagcc acttttagat tttgccgcca
    33121 gttcactgtc gttaccgctt tatcactaat agatcatcat cttctttcca tcgggccttt
    33181 gattattata acacatttct gccaaaaaca gtgtcccata atagtgctaa gtctcatctg
    33241 atagccttca tcgtttacgt aagctaccca gtaaacctct gaccagttct cgcccaattg
    33301 ttctaaccgt cgtactaata aacgcatctg ttgctttttc gacgactgtt ttgcggggac
    33361 gcccaacaga acggcgatta gcttttttgg tagcagcttg tgccttgatg gcttctttag
    33421 cttctttttc gcgttgtgct gtcgctatcg cttcttgggc cagtcggttg tcttctaaaa
    33481 ccttagcagc taatttttca taagcagaat cacgatcaat ggactgacta tatttactgg
    33541 caaaaggtga ggactgcaca agctgctgac tttctatatc tgacaaaaca gcaaagctac
    33601 tcttaggcgg catgatatag gcacgttcaa cgatggatgg ttgtccctta tcattcaaaa
    33661 cagaaatcaa agcttcgccg acttctagtt ctgtaatcac ccttgccaca tctaagtcag
    33721 gattttgtcg gaaagtatcc gcagcgacac gaaccgcctt ttgttctttc ggtgtatagg
    33781 cacgtaaggc atgctgaata cgatttccaa gctgagccaa aaccgtttct ggcaaatcaa
    33841 gcgggttttg agtcacaaag aaaatcccaa ctccctttga acgaataagt cgcacaatct
    33901 gttcaacttt ttccaagaaa actttagggg catctttgaa gagcaagtga gcttcatcaa
    33961 aaaagaaaac catcttaggt ttgtccaaat ccccaacttc tggcaataat tcgtaaaact
    34021 cagacaagag ccaaagtaag aaggttgtgt aaagggtagg agattgaaaa agcttagtcg
    34081 ctgacaaaat gttaatggct ccataacccg aagcaacatc taattgcatc aaatcagcca
    34141 catctaaagc aggctctcca aaaaattggt gggccccttc ttgttctaat gtcaagagac
    34201 ttctttggat agccccgata gattgcttgg cgatattacc gtaatggctg gagtagtctg
    34261 aggcatggtc accaacttct tttaagatag cctgcaagtc tttcaaatca atcaaaagcc
    34321 aacctttctc gtcggcgatt ttaaagacga tgtttaagac acctgtctgg gtatcattaa
    34381 gattcaataa acgcgacaac atcatcggtc ccatttcaga aattgttgtc cgtaagggtt
    34441 gaccgttttg accaaaaaca tcccacattc ttacaggaaa ggcctgaggc tggtaatcac
    34501 ttaccccgat tgtggctaaa cgcgcagcca atttatcagt cacctctcct gctttagtaa
    34561 gattagacag gtctcctttg atatctgcca aaaagactgg cacaccagct aaacttaatt
    34621 gctctgctag cacttttaag gtcacagttt tacctgttcc agtcgctcca gcaatcaaac
    34681 cgtgacgatt tagcatccct aattgtaagg ctatcggttg actgcctttt gcaattgtca
    34741 ttgtagtcat aggcttcctc ttttttgatg atcatacttg tcgatatgtg tttacccata
    34801 agttaaggtg tttagaagtg tctcgctctt aatactatta taacatataa cccttgatgc
    34861 gctaatttaa aagggaccag ctagttttag acagaaatag cttgattttg acagctatga
    34921 tacgaatctc tcagcttagc tacctcggat tggctaatga caacaacgca gattgtactt
    34981 gctttattaa cgtttccatg tctccattat tatcaatcac tagactggca tagggctttt
    35041 tttcctctat tggtaactga ctggctattc gctgtcttgc ttttcctttg tctaaacgat
    35101 ttctagccat taagcgctgc aattgtgtct gagcatctac ataaaccagc caaatcgcat
    35161 caaaccagtc ttggtagccc aattccatta aaaggggaat atccatgaaa aaaatggctt
    35221 ggctctgtgc taagtggtca cgcttagccg ccaattcttc cttaatgatt tgattttgaa
    35281 tggctgagga tgttgccata ttatttgggt ttgaaaaaag catttctgac agctttgtgc
    35341 gatctaactc accatctgct tttaaaatct gattcccaaa agcttctcta agagcctcgt
    35401 aaagacgacc gcctttttct tgcaaatcat ggaccacttg atccgcatca atcacctgat
    35461 aaccagcttt tctaatgacc ttaaccacag tcgattttcc tgatgcaatg cctcctgtga
    35521 tgccaataat catggtcttt ttttctggca ttttggacaa atgtgcgtcc cccttcctcc
    35581 aacttttaat ttgacaatag cctgtccaca acgaggacac ggtttcccag tttgaccata
    35641 aacctgcaaa tagtcctgca tggtgccatc agctcccaaa gcattacggt aagtacggac
    35701 ggtcgaaccg cccttttcaa ttcctaaagc taggattcgt atggtttcat catgcagccg
    35761 ttttatctcc gccttattca acctagaaga cgcagtttct ggatgaacct tagcagccca
    35821 aagtacctcg tctacataaa tattccctaa accagcaacc aaggtctggt ctaacagatg
    35881 tggttttatg ggtttttggg aggatagcag agcagcctca aaggttttta acttaaaggt
    35941 ctctttcttt ggttcagggc ctagttttcg tttggcaaaa aatgctgata attgagattt
    36001 tgctatcaaa tcaaaggttc caaatttacg cacatcttgg taaaccaagg tcgaaccatt
    36061 tttcaattca aaaaagacat gaaagtgttt gttatcaggg acctcatctg gaaaaagcaa
    36121 atatttgcct tccatccgca aatgtgatac caacactaat tgccccaaat caattaataa
    36181 atattttccc cgacggccaa ctgactgaat gatttgacca ggtagggtca gagcaaacgt
    36241 ctctaagtcg gttttaacca tttttggtac tttaagcgta acagccacaa tttcttggcc
    36301 aagcactaaa gtctcaagcc ctcgtcggac cgtttcaact tctggtaatt ctggcataat
    36361 ccgttattta aaggacagct agaccctttg ctgctacaaa gaataaactc agtataaaaa
    36421 agtcactcaa tagactcagg tctaggctac tgactgcgtt tcaaaaaaca cagctgtcct
    36481 tctcctttct tatttcttat tttacgaaca catctgatag aaagcaactc ataaatctgc
    36541 ctcagaccag tcctcatccc ccttacctcc cagcactatt tgacggtaat gttccttata
    36601 actataatac aatgagtcct ctccaagata cttaaaaatc tgcaaggcct gccgcatgtc
    36661 ctcttctcca gacatttctt cctgctttaa tttataatac ccatgcacat ataagaaaac
    36721 agtcttttca taaaaattga gctcgtctcg cagcaaaaga tcaattttgt taatcaaata
    36781 gcgactatgt tcaaagcaag aatggtcaat actgataatg agacaattaa tggataactg
    36841 agtcactaac attttattgg ttttattttg ttccgaataa gcaaaagatg ctaccatttc
    36901 cttagtcaat aaaaataggg tattgtaatt catgaagcgc gagcaattcc ccagaagaat
    36961 gatttcataa tatccccact gttccacttt aaataaataa tctgtgaggc gagtcaattc
    37021 ttcttgacta ggtgcaattg aggagtcaat agaaaaaaga atagctttaa tcatcgttcg
    37081 ttcataatct ttatgggcat aatccttcaa cagtttggtc aatttaacca cgtttttctc
    37141 agcataacat tttctcgcct gacttaagag cgtgaaaaaa tgcgtatgcg tcttggaatg
    37201 agccgaaaca aattcgtcaa tcgtaatatt cagtttatcc aaaagattaa gcaagcgact
    37261 gcaggtgatt tctgattccc ctcgttcaaa tcttgaaatt tgtgacttgg acaggtattc
    37321 atctgctaaa aaagaaatgc taacctgctt acccttacgt aatcgcctta aggtttttcc
    37381 gagttctttt tccatttttc ccactttcac aacaaaaaat taaaatctgc taaatccatt
    37441 atataataca agtacaaaat agaaaatcag aaaggagaca gtcatgaaaa aagttaataa
    37501 agctttgctt tttacattga tcatggatat cctgattatc gttggtggat aacctttcgc
    37561 ttaaaaacaa tccacttttg ggaaaccctt atatttagtt tgtaacagga aaactaatag
    37621 atattaaaaa gagtgttagc gacctagcgg cagctttact cttaagtgcg tttgctgcca
    37681 ttgacagcaa ggtcaaagct gatgaaacag caggggcata ccgttatgat gtgtctaaac
    37741 aaaacactta ctatcaaact atcagctcca ttaaccagca acgacctggt gccactctcg
    37801 acgaatgagg tgattattat ggaaccctta cttcatttac ttcccaaagt cgtaccagta
    37861 cttatacact tggtagacaa agccaacgta tcatggttaa cagtgatatt acagttacct
    37921 atactcttaa agacaatttg gtagcccttc ataaggaata taaaaccttt gtcaaaaaaa
    37981 tattacagat aaaccgatca ccagtgacta cgacaaggct aaggttattt ataaccattt
    38041 aatcaacgaa tacacttacg ccacaaaata acttagcacc aatcgcaaaa ccgtaagcag
    38101 catcaacaat catactccta aagctcttta tcgtggcaag tgtggtgctt gtcaagctta
    38161 tgccgtcatg tttaaagata tggcagaagc tgcaggctta tcaacttggt atgtgataaa
    38221 aaaggattga atgagcacca tacttgaaac atcgttacca ttcatggtgt taattcctac
    38281 gttgatgcta cctggaatga ttcagcatac accacaaggt atttccttgc tggaaaagac
    38341 gtgaggaata ctgagtaccg tttagatagc cagtacaacc acttggctaa tactatttta
    38401 aataccagct accaaaaagg ctaaactaac aacaactaca tttttcaact agaaaggcct
    38461 aaacaatgac caaaaaacag ttttatctta tcttagctat tagcatactg tgttttattc
    38521 ttgccgtcag ccctaaacca ccaattccta aacacactat tgaaaggaaa aggactacga
    38581 tgaccatagg ccactcaaaa caaaaccctc acaaaaaaga agcccttaaa cacaaaaaaa
    38641 ataaaaataa ggctctattt cttccaagtg gggacagcgc tatcaaatct gttattagca
    38701 tgacagttag actcctgtta gatgaccaat cttagctctc ctaaaatcag tggacactac
    38761 catttcggta gtgttctttt ttaagctaat ctcatcacca cgctttcttc aagctaaaaa
    38821 actgatcatg aaagagcaac cccatcaccc taacagccca ctttgcccgc actttcttaa
    38881 aaagttagga gaaaacgata taccaattat tttctgatcc cagctcccta tcattaacaa
    38941 gatgtgtagc acggacctac atacaggtaa gctagagtca ttctcgctat ttcggacaaa
    39001 aagaggccgg tagttccctg acctcttctt tatttatctt gtaacactcc tcttcttagc
    39061 gagacttact agcacataat tcttaagcac tcttatcatt tagactagga ataacaccat
    39121 ctgaatgaag caaatgatgc gccagatctg ccagagtatc atctccataa gtaatccctg
    39181 tttggtgctg tttctgatga aaatcagatg actccttaac tttagcagtc acctgctggt
    39241 cacttggatg aaaattaatt tctctcttgg cttcagccgc aactggctcg aggaacaact
    39301 tattgacatt acggtcttcc tccaaatgat taaggatagc agcatcatct tctaacgaaa
    39361 gaaccttctt gggtaacaaa aaggggttat ttagccttgg ctcagtaacg acttccttgg
    39421 ctgctcgtga tagtttatta ttgtcttcct caggtatcaa acgattaagc aatcgatgca
    39481 attcttccgg ctatgaggcg ctcacagcta cttcagaaga aacctccaag ccctttcctg
    39541 tttgcctgtc taaagcaacc ggcaaattat taactaactt tttaacctgt cctcagcgct
    39601 catttcaaca ctaggaagag ctgtcgctgg ttctagccag ctaccaactt ctgctggtct
    39661 taccttaaca tcgctggcag gcttctcctt ctcatcttca ttaggtctaa aaggctcaaa
    39721 ccgccctgtt gatttttcat ctatgacctt tatattgtct tttactacat gagacagtgg
    39781 ttcgacattt accatagctt catgctcgtt aaggctctgg tctcgtttga atttaccagg
    39841 aataccagga gtcggtgttc caacagattg atcggcttca gtaggctctt ttacggagcc
    39901 ttttttccat aaaactgctg gaggaataat atccgtagag ccatccccat aagtaatcgt
    39961 ggcctgcctc ccctttggat taatagatac tttaatattg tctgggaaag ccccactacc
    40021 tcctgtggtt aaaagggcac ccgcattttt aatgagagtt gtcattcgtt caataccagc
    40081 aacgccaata tggttcaagt catctactgg taactgatca acagatttca tggtgtattt
    40141 ccaagcttgc ggttgaacaa gaaaaacagt tcctttatac attgtgttat gcatatcatc
    40201 aatgactttg aaataccttg tatatttggt ttgtacatta gggttagcaa cgggaacagt
    40261 gctttcagga actcctgaca tcgtaaaaat ccctctagca gaagtttgaa agccctcata
    40321 aacatgaccc ataccaggaa cagtctgagc ggtcaaagac ttaccagaac catccaaagc
    40381 aatgcgaata tctttcatca gatacatctg attggtatag gtaaacctaa ccgtgttagc
    40441 ctcacctcga tacaaaacca cttgggaggg ctgatccgaa gaaaactgag aggttgtttc
    40501 ttccttatag ccaactccaa aacgtctatc agacgaatgg actgaatctg ccgcaactgt
    40561 actactcaaa ctgactagta aagtagccgc acaaccaact aataaactgc tcgttttaac
    40621 tttaacatct cttaacatca tcatgttcct ttcattattt tttatttagg aattcgtcat
    40681 actatatttt ctcatattag tctaacaaaa aactcgtaat tctatataaa tcatataaaa
    40741 tgacgagctt tggcaaagat gagtatagca ggatggaaaa cagattgttt ctgaaacttc
    40801 aaaggtatct tcttgtcacg aactatt
//
LOCUS       DAUGHY010000013        40680 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.013, whole genome shotgun sequence.
ACCESSION   DAUGHY010000013 DAUGHY010000000
VERSION     DAUGHY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40680
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.013"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            47..1243
                     /locus_tag="VH752_000630"
     CDS             47..1243
                     /locus_tag="VH752_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ8289003.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPARKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMVIVLCSVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTTVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGVASFRHLFWLAAVIPLICTLLYLLKTK
                     TRQVVS"
     gene            1745..2593
                     /locus_tag="VH752_000631"
     CDS             1745..2593
                     /locus_tag="VH752_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ8289004.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSRKASYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
     gene            complement(2929..3825)
                     /locus_tag="VH752_000632"
     CDS             complement(2929..3825)
                     /locus_tag="VH752_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEQ8289005.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYFGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            4123..4875
                     /locus_tag="VH752_000633"
     CDS             4123..4875
                     /locus_tag="VH752_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEQ8289006.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKTNQPKEIEQLLAHGAHYDALFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            4860..5810
                     /locus_tag="VH752_000634"
     CDS             4860..5810
                     /locus_tag="VH752_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEQ8289007.1"
                     /translation="MVYEMTLAVIKCIENHLHKKVYKGASLALFQSGRWQEYHIGTID
                     GRRPVDANLVYDLASVSKVVGVATICNILLNNGTLALDDPLKVYYPSIADTTVTIRQL
                     LTHTSGLDPYIPNRDVLNAQQLRKALNHLTQKENKNFYYTDVNFLLLGFMLEELFSES
                     LDQIFDKTIFTPFGMYHTSFGPRPEAVPTLKGVSDGEVHDPKAKILKKHSGSAGLFST
                     LADLESFSNHYLNDPFSDCLWRNYSQQTSERSLGWNLDGDWISHTGYTGPFLMLNKKE
                     QTAAIFLTNRTYDEDDKSKWLKERQLLYNALKHDLTTPVS"
     gene            complement(5990..8317)
                     /gene="pflB"
                     /locus_tag="VH752_000635"
     CDS             complement(5990..8317)
                     /gene="pflB"
                     /locus_tag="VH752_000635"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEQ8289008.1"
                     /translation="MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLEYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
     gene            8526..9620
                     /gene="dinB"
                     /locus_tag="VH752_000636"
     CDS             8526..9620
                     /gene="dinB"
                     /locus_tag="VH752_000636"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEQ8289009.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNLALKGKPVVIGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGEQIRRI
                     FKRYTDVVEPMSIDEAYLDVTNNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVEKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKARGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNAKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGVTMTNLEDKVADISLDL"
     gene            complement(9713..10195)
                     /locus_tag="VH752_000637"
     CDS             complement(9713..10195)
                     /locus_tag="VH752_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289010.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGFFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSVLNVLAIIAIIIFCTQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTLSMVGTILILIQAALYGFAFYKAKTLNEKGDDTDQAML
                     "
     gene            complement(10286..12739)
                     /locus_tag="VH752_000638"
     CDS             complement(10286..12739)
                     /locus_tag="VH752_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEQ8289011.1"
                     /translation="MEYVFTGTVDRIIFENQANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFAFQAFEKYKEEALDLVKENPYQLVEDLQGFGFKMADALAENLGIESDSPKRFR
                     AALLHCLLEESVSRGDTYVQARQLLDFAITLLEDARQVECDPAAVAEQLSELIIEGKI
                     KNSDTKLFDASLYFAEEGIASNISRLLDTPLSQSFSHDTIQTTIQAVQKDFAITYDQV
                     QQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANLHQIDLDKKDLPILLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCDLLIVDEFSMVDTWLANQLLG
                     AINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQIALQKIFRQSQESTIVNLADQ
                     MRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSAIKSGIPAEEIQILAPMYKGQ
                     AGINHLNQLMQELLNPLQGQTEFLFNDTHFRKGDKVLHLVNDAQLNVFNGDIGYITDL
                     IPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRLLQRNVIYTAITRSKSKLILLGEYTAFEYAIKHEGDKRQTYLIERFQEQSDLASS
                     QPNQELKSKEQTSLFSNTATLEDDSQKSSSQSTNSNPTENSQSDNDDFRLTPENYSTI
                     DSMIGLTESDIALFFQKKS"
     gene            complement(12797..13390)
                     /gene="lepB"
                     /locus_tag="VH752_000639"
     CDS             complement(12797..13390)
                     /gene="lepB"
                     /locus_tag="VH752_000639"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ8289012.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIVVAQEEENGQKKEIVKRVIGLPGDTISYNDDTLYINGKKTVEP
                     YLAEYLKQFKNDKLQKTYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGD
                     DRIVSRDSREVGSFKKENLIGEVKARFWPLNKMTVFN"
     gene            complement(13401..14303)
                     /locus_tag="VH752_000640"
     CDS             complement(13401..14303)
                     /locus_tag="VH752_000640"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEQ8289013.1"
                     /translation="MNTLVLKIDAILSKHLKKQLASYTISSQNTYVAFAAKKNGVTVL
                     LYKSGKLVLQGNGANALAQELNLPVAKTVFEASNNSQDIPIIGSDEVGNGSYFGGIAV
                     VASFVDPKDHSFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGK
                     SKPYNAISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKNHFPNPLT
                     FQEKAESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYG
                     MSSLEYSAKLHFANTHKAQALLTK"
     gene            14460..14768
                     /locus_tag="VH752_000641"
     CDS             14460..14768
                     /locus_tag="VH752_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289014.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETVAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
     gene            14771..15316
                     /locus_tag="VH752_000642"
     CDS             14771..15316
                     /locus_tag="VH752_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEQ8289015.1"
                     /translation="MLSLLIVLVLTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLDYFDNQWTKCLSGGLALLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLARL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            15465..17804
                     /locus_tag="VH752_000643"
     CDS             15465..17804
                     /locus_tag="VH752_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEQ8289016.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELLELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHTFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISDDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKITVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADRNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            17808..18308
                     /locus_tag="VH752_000644"
     CDS             17808..18308
                     /locus_tag="VH752_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ8289017.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDCPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            18389..18703
                     /gene="trxA"
                     /locus_tag="VH752_000645"
     CDS             18389..18703
                     /gene="trxA"
                     /locus_tag="VH752_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEQ8289018.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(18755..19342)
                     /locus_tag="VH752_000646"
     CDS             complement(18755..19342)
                     /locus_tag="VH752_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8289019.1"
                     /translation="MEIGQQIIRYRKQQALSQEKLAEKVYVSYQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFKRDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQPYKEILAVSNGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVAFAFASRFMFTWLFH"
     gene            complement(19418..20542)
                     /gene="mutY"
                     /locus_tag="VH752_000647"
     CDS             complement(19418..20542)
                     /gene="mutY"
                     /locus_tag="VH752_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEQ8289020.1"
                     /translation="MWDNETIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            20740..21033
                     /locus_tag="VH752_000648"
     CDS             20740..21033
                     /locus_tag="VH752_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289021.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            21206..21496
                     /locus_tag="VH752_000649"
     CDS             21206..21496
                     /locus_tag="VH752_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEQ8289022.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            21518..22009
                     /locus_tag="VH752_000650"
     CDS             21518..22009
                     /locus_tag="VH752_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ8289023.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            22174..22413
                     /locus_tag="VH752_000651"
     CDS             22174..22413
                     /locus_tag="VH752_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEQ8289024.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(22546..23202)
                     /locus_tag="VH752_000652"
     CDS             complement(22546..23202)
                     /locus_tag="VH752_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEQ8289025.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQRAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLALLIIGGVSLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(23334..24278)
                     /locus_tag="VH752_000653"
     CDS             complement(23334..24278)
                     /locus_tag="VH752_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEQ8289026.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISDLRAPLDVEETSRIAVEDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITT
                     CLHDMTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLE
                     AATRNEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNF
                     QNNWLPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            24481..27309
                     /gene="uvrA"
                     /locus_tag="VH752_000654"
     CDS             24481..27309
                     /gene="uvrA"
                     /locus_tag="VH752_000654"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEQ8289027.1"
                     /translation="MQNKIIIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPIVRRKK
                     GQHKTIFEKIQKDGYVRVRVDGDIFDVTEVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLREGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVIYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEIIASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKEKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGVGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            27425..28498
                     /locus_tag="VH752_000655"
     CDS             27425..28498
                     /locus_tag="VH752_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ8289028.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRRVLITDSRYTLLAKASVEGFDIIESRTPLKVVAELLEADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRFIKDTSEIDTIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIVASGYRSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIAKAIAGMTYSDFDGIPRQL
                     ITEAGYGSHFTHGIGHGIGLDIHENPFFGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITKTGCQVLTLAPKELIVL"
     gene            28533..28994
                     /locus_tag="VH752_000656"
     CDS             28533..28994
                     /locus_tag="VH752_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEQ8289029.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGITKITYKAHYRPHPFAIELMEKKGVAYVQHDVPQIVLGGDKEQ"
     gene            29090..29647
                     /gene="efp"
                     /locus_tag="VH752_000657"
     CDS             29090..29647
                     /gene="efp"
                     /locus_tag="VH752_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEQ8289030.1"
                     /translation="MIEASKLKAGMTFEAEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            29693..30082
                     /locus_tag="VH752_000658"
     CDS             29693..30082
                     /locus_tag="VH752_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ8289031.1"
                     /translation="MTTEYIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            30075..30527
                     /gene="nusB"
                     /locus_tag="VH752_000659"
     CDS             30075..30527
                     /gene="nusB"
                     /locus_tag="VH752_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEQ8289032.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTG
                     EDAQVLELPIFLLSLVTGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSEAPSANKS"
     gene            complement(30668..31633)
                     /locus_tag="VH752_000660"
     CDS             complement(30668..31633)
                     /locus_tag="VH752_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ8289033.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEIIKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(31638..33077)
                     /locus_tag="VH752_000661"
     CDS             complement(31638..33077)
                     /locus_tag="VH752_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEQ8289034.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGHYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRDANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIADLDFDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGGV
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYDYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFTDQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTVRSCQIPQGHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTQGQTGIQVVKGQAFGHYYEMRY"
     gene            33319..35202
                     /locus_tag="VH752_000662"
     CDS             33319..35202
                     /locus_tag="VH752_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ8289035.1"
                     /translation="MDNRQIAAEVIEALGGRENVRSVAHCATRLRVMVYDEGKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYDEVVALGLPTSSTSEQKAEAGKQGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEYGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSTLGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGGIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAATAAQAGATLAVAVKTKSTKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGQLLQYLVTMLVGLGVAFAIA
                     YTWGYQDRETLPLPAVEVDQTADQPALAEETLYSPLNGTVVDLAAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKKGDLLGHFDPSKIAEAGLDDTTMMIVTNSADYQSVDILAQGHVLIGDQVA
                     LIK"
     gene            35433..38420
                     /locus_tag="VH752_000663"
     CDS             35433..38420
                     /locus_tag="VH752_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEQ8289036.1"
                     /translation="MDKHLLVKRTLGCVCAATLMGAALATHHDSLNTVKAEEKTVQVQ
                     KELSSIDSLHYLSENSKKEFKEELSKAGQESQKVKEILAKAQQADKQAQELAKMKIPE
                     KIPMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKEVDLAFIFHDWTKDYSLF
                     WKELATKHVPKLNKQGTRVIRTIPWRFLAGGDNSGIAEDTSKYPNTPEGNKALAKAIV
                     DEYVYKYNLDGLDVDVEHDSIPKVNGEASDENLKRSIDVFEEIGKLIGPKGVDKSRLF
                     IMDSTYMADKNPLIERGASYINLLLVQVYGSQGEKGVFQNDTKLVTDTPEERWQGYSK
                     YIRPEQYMIGFSFYEENAQEGNLWYDINSRKDEDTANGINTDITGTRAERYARWQPKT
                     GGAKGGIFSYAIDRDGVAHQPKKYAKQKEFKDATDNIFHSDYSVSKALKTVMLKDKSY
                     DLIDEKDFPDKALREAVMAQVGTRKGDLERFNGTLRLDNPAIQSLEGLNKFKKLAQLD
                     LIGLSRITKLDQSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPVSLKVSGLTGLK
                     ELDLSGFDRETLAGLDAATLTSLEKVDISGNKLDLAPGTENRQIFDTMLSTVSNHVGS
                     NKQTVKFDKQKPTGHYPDTYGKTSLRLPVANEKVDLQSQLLFGTVTNQGTLINSEADY
                     KAYQNQQIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTLATDKEETYKVD
                     FFSPADKTKAVHTAKVIVGDEKTMMVNLAEGATVIGGSADKEMSRNVFDGSLGGDRSN
                     LLGMSGPKSTIIFNLKESGIVKHWRFFNDRARSPQNPAEDIQEIKLQVFDSEKYDIET
                     LLKTPNQFDKDDYWITVDSYKSKSETVNNYDQVLKMNSAKYWRVTVDTKGGNYSWPSL
                     PELQILGYPLPNADTIMKTVTIAKGLSQQKDKFSQKMLDELKIKEMALETSLNSKIFD
                     VTAINANAGVLKDCIEKRQLLKK"
     gene            38684..39568
                     /locus_tag="VH752_000664"
     CDS             38684..39568
                     /locus_tag="VH752_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ8289037.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFPTTTPEETIAQT
                     IAYFKTFEADLAGMAIGSFGPIDIDPSSETYGYITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     PHPDDMAKGFLGVCPFHKGCLEGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQVIVFGGGVMAQEHMVLRVHDKFTTLLSGYLPVPDLTDYIVTPAVADN
                     GSATLGNFALAKLAAQGK"
     gene            39663..40616
                     /gene="manA"
                     /locus_tag="VH752_000665"
     CDS             39663..40616
                     /gene="manA"
                     /locus_tag="VH752_000665"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEQ8289038.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GREHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATMVLDNMVATTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
BASE COUNT        12325 a         7218 c         8613 g        12524 t
ORIGIN      
        1 ctttttttgt aaaatgttta tgaaaataga gaggagttca ttttcaatgg aagaaaaatt
       61 gtttaacaaa cattttgtgg ccatcacagt aataaacttt atagtctata tggtctacta
      121 tctttttacg gttattattg cctttgtggc gactagagag ctaggagctc agactagcca
      181 ggcaggattg gcaacaggga tttatattct agggacttta ttagcccgtt tgatttttgg
      241 aaagcagttg gaggtgtttg gtcgtcgttt ggttttaaga ggaggtgcta ttttttacct
      301 cttaacgacc ttagcctact tttatatgcc aacgattagc atgatgtact tggttcgttt
      361 cttgaacgga tttggttatg gtgtagtatc aactgcgaca aataccattg taacagccta
      421 tattccagct cgtaaaagag gagaaggaat taatttttat ggcttgtcaa ccagtctagc
      481 agcagctatt ggcccttttg taggaacctt tatgttggat aatcttcata ttgattttcg
      541 catggttatt gtcttatgta gcgtcttgat tgggtgtgtg gttgttggtg cttttgcttt
      601 tccagtcaaa aacatgtctt taaatgcgga gcaattggct aaaaccaaat cgtggactgt
      661 ggatagtttc attgagaaaa aagccttgtt tatcactgca atcgcctttt taatggggat
      721 tgcctatgct tcagttttag gatttcaaaa attatacacg tctgaaattc atttgacaac
      781 agtaggcgct tatttctttg tggtctatgc cttaatcatt accatcactc gtccagcaat
      841 gggacgttta atggatgcta aaggcgataa atgggtcttg tacccaagtt atcttttttt
      901 agcaatggga ctcttcttgc taggtagtgt atcgtctgga ggaagctatt tgctttcagg
      961 agccttgatt ggctttggtt atggcacttt tatgtcttgt ggtcaagccg cctccataca
     1021 aggagtggat gagcaccgct ttaatacggc tatgtctact tatatgattg gtcttgattt
     1081 aggtttaggt gctgggcctt acctcttggg cttgattaaa gatttagcac ttggtagtgg
     1141 tgtggcttcc tttaggcact tattctggtt agctgctgtg attccactga tttgtacctt
     1201 gctttacttg ttaaaaacaa agacaagaca ggttgtgtct taaggacaag aaaaggagtt
     1261 tagtcttata cttaaataca aacgtatctt aacagctgta gatagttcct atgagccggg
     1321 attagctgtg ggtaaagcca ttactatagc gctttgaaat aattctagtt tgctattcgt
     1381 acatgtcaat aaaaatgact cgcaaaagcc attcgtgaaa gagaaaatgt ggccttattt
     1441 ttagtaggtg caattggatt aagcacgttt agatacatga caattggatt aacatcagaa
     1501 tataggcttt gtcatgttaa gattgatctg ttgatagtga gagctaataa aaaatgttat
     1561 aatactatga aataaagtga taaagataag gccttttatg gtccttttct ttaccgcttt
     1621 tattattgtg tgaaaataac acttaatgat cattttattt attattttat tacctttaca
     1681 ttcatcaata agcctgttaa aatggtattt gttatatttt attatttgga ggaaaccaga
     1741 aaacatggaa atgacatgga ctgtgaaata catcacagaa tttatcgcaa cggctttttt
     1801 gattattttg gggaatggag ctgttgctaa cgtcgactta aaaggaacaa aaggacataa
     1861 ctcaggttgg ttagtgattg cttttggcta tggcttagga gttatgatgc cagctttgat
     1921 gtttggtaat gtatctggaa accatattaa tcctgctttc acagttggac tagcagtatc
     1981 aggcttgttc ccatgggctc acgtcttaca atacgttgtg gcacaattac ttggagctat
     2041 ctttggtcag ttggtagtgg taatggttta taaaccttac tttatgaaaa ctgaaaatcc
     2101 aaatcatgtt ttaggctcat tctcaacgat ttcatctctt gataatggtc aaaaagacag
     2161 ccgtaaagcc tcttatatca atggtttctt aaacgaattt gtaggttcat ttgttctttt
     2221 ctttggtgct ttggctttaa ctaaaaacta ctttggggta gagttagttg gtaaattgat
     2281 tgaagcaggc tacgaccaaa caactgcagc aacacaaatt tcaccatacg tgacaggctc
     2341 attggcggtt gctcacattg gtattggttt cttagttatg gtcttggtta cttcattagg
     2401 tggaccaact gggccagcac taaacccagc gcgtgatttt gggcctcgtt tgttacacca
     2461 cttcttacca aaatcagttc ttggtcaagc caaaggtgat tcaaaatggt ggtatgcatg
     2521 ggttcctgta gttgcaccta tcttagcggc gatagtagcc gtagcagcgt ttaaatacct
     2581 ttatatcaga taaaaaaaga actaaagctt cttttagact caagacaagc tcgcttaagg
     2641 aacttgtctt ttttagatga gttaagcgaa atctaatgca aacttgactg tcttgtatag
     2701 cgtattttgt aaattttatt agacttcttg taaaaaagcc tctcctcaga taagatgact
     2761 agtgcctcta aacggtgtta caaataaacg aggcatccct aacttccttt atctggtgtt
     2821 gaaactattt tgcacggact aaatcactga cagagtaaaa aacgattttt catttttgtt
     2881 gtaaaatatt aaaaacgtct ctggttagtg attcccagaa acgttatctt atctatttgt
     2941 ttgttgctta tgagaggtaa aggaggaacg aatcagcatt ccaccagtga agaaaacgat
     3001 agcgatgacc agaagtttca agctattaat atctaaattt gttaagaaaa acacagctag
     3061 taaaacgcca ataataccac cagaaacaat tccagcaaaa ccatcccaac tgacacggtt
     3121 tgctttgata aactggctac tagaagctgt cataatcata gctgcatcta gcatcataac
     3181 aggcatagca actgttgggc taagacccat taatgaaaag aaaatcaatt ctggagcata
     3241 attgcctaac cccatagtca tcaagactcc aacgataaaa ttaaaaccaa ttccaacaaa
     3301 aagccagatg ccatggaggc catgaatggt gtctgaaagg tgttctcctg gatggtagat
     3361 tattctaatg atcatgatta tagctgctat gattaaaaga ctaccaagga ttcgttggac
     3421 agttggagca tgccagtttt ttgtaatatg agtcccaaaa taagctccaa taaaggctgc
     3481 tgcagccata gcaagtaaag ttagaacctc aacttttacg acaaaaataa agcaaagaga
     3541 ttgaatgagc accggaagca catgagctac ggtcatagtg ccaggcaatt gtctatcgtc
     3601 agtgactaat ttagttaact taaagcacgt tgtggtagta gcaaaagacc ctatcccaag
     3661 ggtgtcaagg gcatctgtta gcaggccaat ccaaaacccc gtccaaaagc gtttaaaagg
     3721 attgatctta ttttttttaa gatgttttac taacataaag aaaataaaaa gcattgccga
     3781 aactagcaaa gcttgtatca gtctaaggat aagcatttcg ttcataaaga ctagtttaca
     3841 aaaaaaaacc ttagccgtca acgtgattta cttcattaaa acatgccact atgcttagaa
     3901 gagctttagt tcgatgtatt ttgttaaaat taagctaaaa tattaatatt gcatgaaagg
     3961 ggacgccttc ctttttccct tactgagctt agctgttatt gtagtgaatg tttaaaaggc
     4021 tagtttgtcg ctctttatca aaaatctagt aggatgaata cgtataattt cttgtcaaat
     4081 agaggaattt tgtctataat agcagaggat taggaggaaa ttatgacttt aatggaaaat
     4141 attaccttta aaactccagt attacgcgtt aatgatcgcg accttaatat tgcattttat
     4201 caaaataatt tagggcttcg tttagtatct gaagaaaatg cgattgctat cttttcatct
     4261 tggggagaag ggcaggaatg ttttgtaatt gaagagtcac cttcagttag aacgcgtgcg
     4321 gtagaaggcc ctaaaaaggt taatacgatt gtcatcaaaa caaatcaacc taaagaaatc
     4381 gaacaattat tagctcatgg agcacattat gacgctcttt ttaaaggtca aaatggctat
     4441 gcctttgaga ctatttcccc agaaggagac cgattcttgt tgcatgctga acaagatatt
     4501 aaacatctcc aaggtactga tttaccatcg cttgaaaaag atgcgacttt taaaggttta
     4561 actcaattta agtttgatat tattgtttta aatgttatta gtgaagaacg ctcaaaagct
     4621 ttttatcggg atctgtttag tgatcagtta cctattacta tggattttat tcaagaagaa
     4681 ggtcccgatt tggcaattga tccccatatt gcttgggatt tagaaatttt agaatttcaa
     4741 gtctcaaagg actatgatat gaaggtccta aaagcaactt tagaagaaga cgggcacaag
     4801 gtctacattg ataaaaaaca caaagtattg gtcctttctg atccaagtca gatcgaagta
     4861 tggtttacga aatgactttg gcagtcatta aatgtattga aaaccatctt cacaaaaaag
     4921 tatacaaagg ggccagtctg gctctctttc aatctggtcg atggcaagaa taccatatcg
     4981 gaacaattga tggaagacga ccagttgatg ctaacttagt ttatgacttg gctagtgttt
     5041 caaaagttgt tggtgtggcc acaatatgca atattttatt gaataatggt acgttagcat
     5101 tagatgatcc cttaaaagta tattatccta gtattgctga tacgactgtc actattcgac
     5161 agttattgac tcataccagt ggtcttgatc cttatattcc taacagagat gttttaaatg
     5221 cacaacaatt aagaaaagca cttaatcatc ttactcaaaa agaaaataaa aacttttatt
     5281 acacagatgt taatttcctc ttgttaggct ttatgttaga agagctcttt agtgaatctt
     5341 tagaccagat atttgataag actatcttta ctccctttgg gatgtatcat acgtcttttg
     5401 gccctcgtcc agaagctgta ccaacattaa agggtgtgag tgatggagaa gttcatgatc
     5461 ccaaagcaaa aatcttaaaa aaacattctg gatctgcggg gctattttca actcttgcag
     5521 atttagaaag ttttagcaat cattatctta atgatccatt ttcagattgt ctgtggcgca
     5581 actacagcca acaaacaagt gagcggtcac tagggtggaa tttagatggc gattggatta
     5641 gtcatacagg ttacacaggg ccgtttctaa tgcttaataa aaaagagcaa acggcagcta
     5701 tttttttgac caaccgtact tacgacgagg atgataaaag taaatggtta aaagaacgtc
     5761 agttgcttta caatgcacta aagcatgatc tgacaacacc cgtatcgtaa cgtctaaagg
     5821 ggcagtttta gtgatcttat tactgttttt aaagtttctt ctaaaaaata gccatttcat
     5881 ttgtctgatg cgattagagt gattataagt gaaaaaagaa cagccatctc attttgaatc
     5941 aaatgagatg gctgttcttt tttataagtt attgatcatt tagatactac tatttacttg
     6001 aaatagcttc agcggcatca tccattgaaa gcacttcgtg gaagacacgt tgagtcaatt
     6061 cagttttttg ttctggtgta aggtatttgg tgttgacaca gtaaccagag atacgcacga
     6121 taacgtcttc gccgttcatg attttgtcat aaacatcgtt aaggtccata acgttcaagt
     6181 taacgtgttg accaccattt tcaaagtaac cgtcaagcac tgttaccaag ttgtcaactt
     6241 gttcgtcaaa tgttttacca agagcacgag gtgatacttg agtagtcaat gagataccat
     6301 cgttagcatg tgagaattcc agtttagcaa gtgagttaag gttttgtaac cagccacctt
     6361 ttgctttgtt agatgggtta gcacctggtg agaagaattc cacttggcta gtgttaactg
     6421 taccatcttc gttcaagaag acgccgcggt gaactggaga gttaccagtt tgtttagagt
     6481 aagcaacgtt tgaagtgatg gtaagaagtg atactgaagc ctcagcattc ttgtaaagtt
     6541 tgtggcttgc aagacgtgtg tggtaagctt ccatcaacca tttcgcgatg tcatcaacac
     6601 ggtcgtcatc ttcaccgtaa cgagggaagt cacctgttac ttcgtagtca tagatgtagc
     6661 cattttcatc gcggatagtt ttaacagttg cgtatttaat agctgacaag gtatcaactg
     6721 tgttagcgaa accacagata ccgaatccca tgttagcacg ttggtgtgtt ggcaagaagg
     6781 ccatttgaac tgcttcgtag ttgtatttat cagtcatgta gtggatgatg ttaagcgcat
     6841 ctacataagt atctgtcaac cagtcaagag atttctcaaa gttagccata acttcgtcgt
     6901 actcaagaac ttctgatgtg attggttcaa caacgttaaa cactttgtag tctctgtgaa
     6961 catcatcgta accaccgtta agaccagtaa gaagggcttt gagaacgttt acacgagcac
     7021 cgaagtattg gatgttatga cgttgttctt cattttctgg gtcaagtggt gatacacaac
     7081 atgaaataca tgacatctca ccataacctt ctttagccat tgttgtcaca ccttcatatt
     7141 ggatagaaga gtgtttgtgg ctcattttca tacagtagcg acggaatgtt tctggcagtt
     7201 ggtcagtcca aagaactgtt aagtttggtt ctggagagtt accgatatta tcaagagtgt
     7261 ttaagaaacg gtagtccatc ttagtcacac ggtgacgacc gtcgttaccc ataccagcca
     7321 tagaagttgt gatgaatgtt gggtcacctg agtaaagggc atcgtaagct ttcgtacgac
     7381 caaattttac ggtacggagt ttaaggacaa aatcatcaac aaattcttgg atttctgatt
     7441 ctgtaaaggt accgcgagca aggtcacgtt ctgcaaagat atcaagaacg attggcacac
     7501 gtccaagaga agtagcagca ccgttgatca cacgacatac agccatgaaa gcgatgttta
     7561 cccattggat agcttcttta acgttttgtg ctgggcgacg aacatcaact ccgtaaaggt
     7621 caccaagttt aaccacttca ccaagagctt ggtattgaag gttaacttct tcgcgaagac
     7681 gaatggattc ttcatcgatt tcagtgatag cattccagtc attgactttt tcttgcatca
     7741 agtagtcagc accgtaaaga gcaagacgag cgtaaacacc gatgatacgt ccacgtgagt
     7801 aagcatctgg aagaccagtt acagtgtggg catgacgagc gcgacgaata tttgaagtat
     7861 aagcgcggaa gataccgtcg ttaacagttg tcacatactt agtaaagatt tcatgtactg
     7921 ctgggtctgg ttcatagccg ttttctttaa gggtcgtttc cgccatacgg ataccacctt
     7981 ttggcatgaa gttcaatttg aacaattcat cgttttgaat accataaatt aattcatttt
     8041 ctttatcgat gaaaccagca ggaatgtcag caattgaagt tggacgagta tcgtatggga
     8101 aacgtgtagc ttcatagtgt gcttttgttt cttcgataac ttttttgatg tgaagtgaac
     8161 gttcagtagc accagccaag aaagattcat cgccatcgta tggggtgtag ttagcttgta
     8221 caaaacgtga gacacttgct ttttctttcc agtcagttcc tttaaagcct tcccaagctt
     8281 tttcgaaaac atctgtattg gttttaacag ttgccataaa tttctcctta aattctagta
     8341 acaaagcgat ctgttacatt tacaattcta agtttaacac ataggaaacg ttttcccaag
     8401 caaaattctt gacaaaatta agattctttt ataatacaga ttgttgaaac agtcacaaac
     8461 tgtattagaa tgtatttttt tggctttaga tagcctaaaa ggtataatag agaaaggagg
     8521 gacttatgct tatttttcca ctgattaatg acacgtcacg aaaaatcatc catattgaca
     8581 tggatgcctt ttttgctgca gttgaggaaa gggataacct tgctttaaaa ggaaagcctg
     8641 ttgtgattgg gaaagatcca agagaaacag gtggtcgcgg agttgtttcc acttgtaatt
     8701 acgaagcgag aaaatatggc attcattcgg ccatgagctc taaggaagct tatgagcgtt
     8761 gtcccaaagc catttttatt tcaggaaatt atgaaaagta tcgaacagtt ggggagcaga
     8821 tccgccgtat ttttaagcgt tatactgatg tggtagagcc catgtccatt gatgaggcct
     8881 atctagatgt caccaacaat aagttgggta taaaatcagc cgtcaaaata gccaagctga
     8941 ttcagcatga tatctggaaa gaagtaggat tgacctgttc agcaggtgtg tcttataaca
     9001 aatttttggc taaattggct agtgattttg aaaaacccca tggcctcacc ctagtcttga
     9061 aagaagatgc cctgtgcttt ttagccaaac tccccattga aaagtttcat ggtgttggta
     9121 aaaaatcagt tgaaaaactg catgacatgg ggatttatac aggacaggat ttgttggcag
     9181 ttcctgaaat gaccttgatt gatcattttg gtcggtttgg ttttgacctt taccgtaaag
     9241 cgagaggcat cagcaattct cctgtcaagt ctgatcggat acgcaagtca attggcagtg
     9301 agagaaccta cgctaaactg ctttatcaag aaacagacat caaggcagag atcagtaaaa
     9361 atgctaagcg cgtggccgct ctcttacaag accataaaaa gttaggcaag accattgtgc
     9421 tcaaagtgcg ttatgctgat tttaccacct tgacaaaacg tgtcaccttg ccagaattaa
     9481 ccagaaatgc cgcacaaatt gagcaagtag ctggggatat ttttgacagc ttaagcgaaa
     9541 atcctgctgg tatccgcctg ttaggggtaa ccatgaccaa tttggaggat aaggtagctg
     9601 atatttcctt ggacctatag gagaaagagc gctctatccg tccgcctcat tttggagaaa
     9661 aaagaagctg attagggatt tccctaatca gcttctttgg tccattattt tattacagca
     9721 tagcttggtc tgtatcatca cctttttcgt taagggtttt tgctttgtaa aaagcaaagc
     9781 cataaagagc agcttgtata aggataagga tagttccaac cattgacaag gttgttgtga
     9841 actggttaac caagccgata acagaaagga taagtcctaa tatgtaagga atataactaa
     9901 ccgttaatcc ttgttttagt ttggataagt tctgagtaca gaagatgata attgcaataa
     9961 tagcaaggac atttaagaca gataagaaaa tcataaatgg tgtcatgctg ctttgtagct
    10021 gtgccaattg ctcagttgtt aattgggccc gaaatgcttc attcttgagt tggctttgta
    10081 agtagaaaaa acctgcaaat cccattaatg acagtacaac tccaagcccg tttagaataa
    10141 taatggcaat agtagacgtt ttgagtgcct gacgcacttt ttcataagaa atcatataat
    10201 cctcctagag tccttaatcc gaaattgtac aatgtgttta atacaaaaat attataccat
    10261 agattaatca aaaatgtccg tttttctagg attttttctg gaaaaagagg gcaatatctg
    10321 actctgtaag ccctatcatc gaatcgatag tcgaataatt ctcaggtgtt aacctaaaat
    10381 catcattatc tgactgagag ttctcagtgg ggttagagtt tgttgattga gaggaagatt
    10441 tttgagagtc gtcctcaagg gttgccgtat tagaaaaaag ggaggtctgc tcttttgatt
    10501 ttagctcttg gttaggttga gaggaggcta agtcggattg ttcttggaag cgttcgatca
    10561 agtaggtttg gcgtttatcg ccttcgtgtt taatagcata ctcaaaggca gtatattctc
    10621 ccaacagaat taacttactt ttagaccgag taatggccgt gtaaatcaca tttcgttgca
    10681 agagtcggcc gctttggcgt gtgataggta aaatcaccac ttgaaactca ctcccttgcg
    10741 atttatgaat gctcatggca taggccaggg ttaattttag ccattcattt ctagggtacg
    10801 tgacttcgct accgtcaaaa tctaagatta attcgtcttg tttagattcg gtgtatttag
    10861 caggaatcaa atctgtaata taaccaatat ctccgttaaa gacattcaac tgagcatcgt
    10921 tgactaagtg caggacttta tcacctttac gaaaatgcgt atcattgaac agaaattctg
    10981 tttgtccttg caagggattg agaagttctt gcatgagttg attaagatga ttgatgccag
    11041 cttgtccttt gtacataggc gctaaaattt gaatttcctc agcagggatg ccacttttaa
    11101 tagcagatag gacaattttt tgaatcatgt cagggataaa ggctgcttgg gcttcaaaat
    11161 aagaacggtc agctttttta tcacgaaagt cagcagctaa gattcctcga cgcatctgat
    11221 ctgccaaatt gacaatagta gattcttggg attgacgaaa tatcttttgc aaggcgattt
    11281 ggggcaggct attgactttt aaaagatctg acaggacttg accgggtcca accgaaggaa
    11341 gctggtcact atctccgaca ataatcactt gagttgttga gttaatcgct cctaacaact
    11401 ggttggcaag ccaggtatcc accattgaaa attcatcgac aatcagcagg tcgcagtcta
    11461 gataatcctc catggcttgg taatcgttgt cgccattgag gcctaagtgc ctgtggatgg
    11521 ttgcgctagg aagtccagtc aactcattca tgcgacgtgc agctcgacct gttggagctg
    11581 ctaacaagat aggaaggtct tttttatcca aatcaatctg atgcaggttc gcgtaagcct
    11641 gcaaaatgcc tcgaataaca gttgtttttc ctgttccggg accacctgtt aggagaaaga
    11701 ccttgctggt taaggcctta gtaatggctt cttgctgcac ttggtcatag gtgatggcaa
    11761 agtctttttg aacggcttgg atggttgttt gaatggtatc atgactaaat gactgactta
    11821 aaggggtatc taagagacga gagatgttgc tagcaatccc ttcttcagca aaataaaggc
    11881 tagcatcaaa cagtttggtg tcactgtttt tgattttgcc ctctatgatc aactcgctta
    11941 gttgttcagc cacagcagca ggatcacatt ctacttgacg tgcatcttca aggagtgtga
    12001 tggcaaagtc taataattgt cgtgcttgga cataagtgtc tcctcgactg acggattctt
    12061 ccaagaggca gtgcaggagg gcagcacgaa aacgttttgg agagtcgctc tcaatcccta
    12121 agttttcagc aagagcatct gccatcttaa acccaaaacc ttgtagatct tcgactaatt
    12181 gatagggatt ttctttaaca aggtcaagag cctcttcttt gtacttctca aacgcttgaa
    12241 aggcaaaacg gttgctaagg ccaagttcga cgagaccagc aatcagttgc tctgtgccat
    12301 aattcaattt cagtttagcg acaaaggctt gacgattagc cttggacaga ccagagatag
    12361 tttctaattt gcttgggtct tctaaaatat ggtcaatggt attatgacca tatagcgcaa
    12421 tgattttctc cgctgttttt ttaccaatcc ccttaaaatg gtcactagag aaataattaa
    12481 ccaaaccaga tgaactaggt ttgatttttt ggtaacggct tagtttgagt tgctgtccat
    12541 atttagggtg ctgggtcaat tctccccaaa aggtgtagtc atctccttca ataatgtcag
    12601 ccatcgttcc tgtgataatg atttcaaagt cgtcaatgtc actgtctgta tcctcaatgg
    12661 caaggaggag aattttaaag aaattagcct ggttttcaaa aatgatacga tcaacagtac
    12721 ctgtaaaaac gtattccata gtaactcttt ctatttatac aagataactg aaaacctgcc
    12781 caaaggcagg ttttttctaa ttaaaaacgg tcattttatt gagtggccaa aaacgagctt
    12841 tcacttcacc gataaggttt tcttttttga aactaccaac ttcgcgacta tccctggaaa
    12901 caatacgatc atcaccaaga agaaggtatt ctccttttgg aacactcatc tcaaagcgtg
    12961 tttgtccctc agaattagtt gtaaaagcat cagatgtttc tgctaactgt tggaataggg
    13021 tattataggc gtaagttttt tggagtttat cgtttttaaa ttgttttaga tactcagcca
    13081 aatacggctc aactgttttt ttaccattaa tataaagtgt gtcatcatta taagaaatgg
    13141 tatcgcctgg caatccaata actcttttta cgatttcttt cttttgtcca ttttcttctt
    13201 cctgagcaac tacaatatca aagcgatcaa tcctagcttg attaaaaacg ataaggcgtt
    13261 cgccatgagc tagagttggg tccatagaat ggccgtctac tttaacagcc tgccacaaaa
    13321 aaagacgaga tagaccaaag agaattaaaa agagagtgaa tgggccccat tctttaataa
    13381 actgtttcat atgattcctt ttatttagtg agaagcgctt gcgctttgtg agtgttggcg
    13441 aagtgaagtt tagcactata ttctaagcta gacataccat aagcggctaa gagttggcta
    13501 gcaactttat ctgaagctga accagcaccg cttgggagtt ggtaacctaa atcttgacct
    13561 aattgatcga gattgtctaa aaatagattt ctagcaatga tagagctaac agccacagcc
    13621 aaatagtgac tttctgcctt ttcttgaaag gttagaggat ttgggaaatg gtttttttcc
    13681 ttttttagat gcttttcata atttgattgg ctagtgaaag catcaataac aatttgttta
    13741 ggttgaatgc ctttttgtaa taacaaaaaa atagcttggt tatgaagagc aactttgata
    13801 gaaatggcat tatatggttt acttttacca accaattcgt tatatttttt aggtgatagg
    13861 agaagtgact gatgaggtat ttgtttctct aacagaggcg caatctgttg aatggtttta
    13921 tccgataatt ttttagaatc atcaacccca agctttttca ggaaggaatg atctttagga
    13981 tcgacaaaac ttgccacaac agcaatccca ccaaaataag agccattacc cacctcatca
    14041 ctgccgatta taggtatatc ttggctattg ttgcttgctt caaaaactgt cttagctact
    14101 ggaagattaa gctcttgagc taaagcattt gcaccgtttc cctggagaac taattttccg
    14161 gatttgtaca gcaaaacagt aaccccattt tttttagcag caaaggctac ataggtattt
    14221 tggctactga tggtgtagga ggctagctgt ttttttaaat gtttactcag tatagcatct
    14281 attttcaaaa ctaaggtatt cataataatt attttaacat aaaatagttg actatggtta
    14341 attcataagt ttatttaaca aagaaaacag agactcgaat gtttagacgg gttagggcaa
    14401 tggtgttgac tattttacga aagtttcatt ttacggtata atgctaatga ggtgacccta
    14461 tgaaaagtat taatcgctac aaattcacct ttggtgaaaa aacattaaca ttgacaactg
    14521 acaaagacaa tctctttatg gaagaggttg agcgggtagc taaagaaaag tatcaggctc
    14581 ttaaaaatca tttaccagaa gctgatgacg agactgtcgc tattttaatg gctatcaata
    14641 ccttgtcaac acaattaagc agagagattg ccattgaaaa aatggaagct gaaattttag
    14701 atttacgtca aaaaaccttg gttggtcttc aagaaaaagc taatcaaata gatgtgaatg
    14761 aggtttaatc atgttatctt tattgatagt tcttgtttta acttggaatt tttatattgg
    14821 ttatagtaga ggtattattc ttcaatcgtt ttatgtctta ggagccctac tttctctttt
    14881 ggtagctaat cgattttata ttggattagc ccataagcta acgctttgga ttccttattc
    14941 taacccggta gaaggaacat cagtcttttt ctttaaatca gttgatatct ttgttttaga
    15001 taaggtttat tatgcaggcc ttgccttttt tatcattttc ttattggggt atgcattgag
    15061 tcgctttctt gggatttttg ttcatttcct tttgctagat tattttgata accagtggac
    15121 aaaatgtttg agtggcggac tagcgcttct agtatcgctt ctttttttga acatgttact
    15181 gtctattttt gcaacggtcc ctatgccttt tttacagcac tatttgcact ctagttttct
    15241 tgctcgtttg gtgatcgaac accttccacc attgacaata attattcaaa aattgtggat
    15301 tcaagccatt atttgatatc gttttgtgac taatataaga acctggagtg agtttcacct
    15361 ttaggttctt tatttacgag aaactgttta gaaataaaga gaacgcatgg gaacttagtg
    15421 actttcttgc cccattttcc atcattttag gtataatgaa gagtatgaat aacaagattt
    15481 tagagcagtt agaatttaac aaagttaagg aattgctatt accttatctc aagacagaac
    15541 aatcccaaga agaattatta gagctggagc cgatgacgga ggctcctaaa atagaaaaaa
    15601 gttttaatga aatttctgac atggaacaga tttttgttga acatcactca tttggcatag
    15661 tcagcctaag ttcaatctct gagagtttaa aacgcttaga gctttcagct gatcttaata
    15721 ttcaagaact tttggctatc aaaaaagttt tacagagttc ttcggatatg attcactttt
    15781 attctgattt ggataatgtt tctttccaat ctttggatcg tttgtttgaa aatttggaac
    15841 aattccctaa tctgcaaggg tcttttcaag ctatcaatga tggtggtttt ttagaacatt
    15901 ttgcgagtcc agaattagag cgtatccgtc gtcaattaac aaatagtgaa cgacgggttc
    15961 gtcagatttt acaggatatg cttaaggaaa aagcagagct tttatcagag aatctaatcg
    16021 ctagtcgtag tggacgaagt gtcctaccag taaaaaatac ttatcggaat cgtatttctg
    16081 gtgtggttca tgacatctct tcttcaggaa gtactgttta tattgagcct cgtgctgtag
    16141 ttacactaaa cgaagagata acgcagctta gagctgacga acgccacgaa gaaggtcgta
    16201 ttttacacac attttcagac ttgttaagac ctcatgtcgc cactattaga aataatgcat
    16261 ggattcttgg gcatcttgat tttgtaaggg ctaaatatct ttttatgtct gataataagg
    16321 cgacgatacc taagatctct gatgacagca cgttagcatt aatcaatgtt cgtcatcctc
    16381 tgttaagtaa tcctgtggct aatgacttac attttgatca cgatttaact gcaattgtca
    16441 tcactggtcc caatactggt ggtaagacga ttatgctaaa aacactcggt ttagcacaat
    16501 taatgggaca gtccggtttg ccagtattag cggataaagg tagtaaaatt acagtattta
    16561 acaatatctt tgcagatatt ggcgatgagc aatctattga acaaagtcta tcaacttttt
    16621 ctagtcatat gacgcacata gtcagtattt taaacgaggc tgaccgcaat agtttagttc
    16681 tctttgatga actgggagca ggaacggatc ctcaagaagg tgctagtttg gctatggcta
    16741 ttttagaaca ccttaggtta agtcatatca aaacgatggc gaccacgcac tatccagaat
    16801 taaaagctta tgggattgag acaaattttg tagagaatgc gagcatggaa tttgatgccg
    16861 aaacgcttag ccccacgtat cgctttatgc aaggagttcc tggacgatca aatgcatttg
    16921 aaattgcttc tcgccttggt ttagctccat ttattgttaa acaagctaag cagatgacag
    16981 attctgactc agatgttaac cgtattattg aacagttaga ggcacagaca cttgagacac
    17041 gtagaagact ggatcatatt aaagaagttg aacaagaaaa cctcaaattc aatcgtgcgg
    17101 ttaagaaact ctataatgaa ttttcacatg agcgcgataa agagttagaa aaaatctatc
    17161 aagaagctca agaaattgta gatatggctt tgaatgagag tgatactatc ttaaaaaaac
    17221 tcaatgataa gagtcaatta aaacctcacg aaattataga tgctaaggca caaataaaaa
    17281 aattagcacc tcaagttgat ttatcaaaaa ataaagtctt aaataaggct aaaaaaatta
    17341 aagcagctcg tgcccctaga attggtgatg atattatagt gactagctat ggacagcgag
    17401 gtactttaac tagtcaatta aaagatggcc gttgggaagc acaagtggga attatcaaaa
    17461 tgacattaac acaagatgaa tttacccttg ttagagtcca agaagaacag aaagtcaaaa
    17521 gtaaacagat taatgtggtt aaaaaggctg atagttctgg accaagagct cgacttgatc
    17581 ttagaggtaa aagatacgaa gaagctatgc aagagttaga tcattttatt gatcaagcat
    17641 tgcttaacaa tatgggacaa gttgatatca ttcatggtat tggtacaggc gttatccgtg
    17701 agggagtgac aaaatatctt cgtcgtaata agcacgttaa gcattttgct tatgccccac
    17761 aaaatgcagg gggatctggc gccacaattg taacgttagg gtaaattatg gaatcatatg
    17821 aacaatttta tgccaaacta tctcaacctt ttagaaaatc cccacagtta ataattcttt
    17881 taaatttttt actaaaaata gtcacaggaa tgatgtacat actctatcca tcttttctga
    17941 tatttacgct ttggcaaggg atgacatttc aattatggtt aaggctatta attatccctg
    18001 ctgttggctt tatagctttg tcttacatta ggaaacgctt tgattgtcca cgaccgtatg
    18061 aaaaatggaa tattaaacct ttaattgata aggatacaaa gggaaggtca atgcctagca
    18121 gacatgtttt ttcagctacg atgattagta tgtgtttgtt acgatattat gtttactttg
    18181 gaatagtttg tttaatatta tcggttctat tggcaatttg ccgtgtgata gcaggtattc
    18241 actatcctaa ggatgttatt gtaggttacc ttattggctt gatgttaggg ctttgtttat
    18301 tgatttagcg agttcgtagc aaatagtttc aaaaacaact aaattcttgt acaataggct
    18361 tatcattaag atgaaatgga gaaaaaagat ggcattagaa gtaacagatg ctacatttgt
    18421 agaagaaact aaagaaggtt tagtactcat tgatttttgg gcaacctggt gtggtccatg
    18481 tcgtatgcaa gcaccaatct tagaacagtt atctcaagaa atcgatgaag atgaactcaa
    18541 aattttgaaa atggatgttg atgaaaatcc ggaaactgct cgtcagtttg gtattatgtc
    18601 aattccaaca ttgatgttta aaaaagacgg agaagtggtt aaacaagttg caggtgttca
    18661 tactaaagac caactgaaag ctattattgc tgaattgagt taactaaaat aaagcccagc
    18721 gtcacgctga gctttatttt attatctact aacttcaatg gaataaccat gtaaacataa
    18781 aacgacttgc aaaagcaaaa gcaacagtaa tgaggaaaaa tacagtgaca ataaggggtt
    18841 tctgataagg aagttttgct ctttcttctc gtttttcgat ttcgtctaat aattttccat
    18901 ttgaaacagc taggatttcc ttataaggtt gcacatcata ttttttctta aaacgttcca
    18961 cacgattagc ataagtcata gtaattatcg ataataaaac aaaaatgcca agtcctagcc
    19021 actctaagaa atagactaat ggataacttg aaatcattaa tagtatcatc aatgttacca
    19081 ttactttggt atctcgttta aaattctttt tatcaacctg agtgatggta tatttcattt
    19141 tctcaatgtc tcctttaatg agttggtcta agctcacttg aaaaatttga cttaggagta
    19201 agaggctatg gatgtctggg taagttttgt cattttccca gttagatatg ctctgatagc
    19261 ttacatagac tttctcggct aatttttctt gcgagagcgc ttgttgttta cggtagcgaa
    19321 taatttgctg accaatttcc ataaatatca ccggaaaact ttacagcggc gtaaaggaat
    19381 tgtaaacaaa aagtgaaccg ttatagttca cttgcgatta agcatttttc tgttttaagt
    19441 aggtctctag cattttcttt tgtggtgttg caaagggata cagagaaaag tcctcaattg
    19501 ctacccactt taggtgagga gcattaggta aatctgttgc tttgaccacc ccctctatga
    19561 gttctattgt ccacttctgg tgactaaaag tgtgcttaat atttggaaag tgattatcag
    19621 tccattgggg ctttaattga tattctcttt cgaaagtttc attttgggtt tgccagatta
    19681 tggggttgga ttgattgtca tcaaataaat ctaattgttg agaaagaggt gacgtttcga
    19741 taataggaaa tgaccaaaaa cctcctagaa gtctcccttt agtatttttc tccagtagat
    19801 attggccatt ttggttacga atgacaaaag cctgaattcg cattggttta ggctttttct
    19861 ttggattttt tatggggtat ttaccataag taccattcaa ataagcagcg ttaaagaaac
    19921 gaatagggct ttcatcaggc ctaggtgtct tagcggattc gatgtctgta cctagatcca
    19981 taagtgcctg gttaaaatca ccgggtctgt cagggtcaat tagtatttcc ataatagctt
    20041 gaaaaatttt acgattttta gggtcaccga tatcataatt aacttcaaac aaacgagcca
    20101 tgacacgcat cacattgcca tcaacagcag gctctggtaa gttgaatgag atgctggcga
    20161 tagcacctgc agtataagga ccaatccctt ttaatgaagc aatatcatca taagtgtgag
    20221 gaaatatgcc accaaaatca accatcactt gctgtgcagc cttttgcata ttacgcaccc
    20281 gagaataata gcctagtccc tcccatgcct ttagtaattg ttcttcagga gcgtctgcca
    20341 aatcttttat ttgaggaaac cactctagaa aacgtttata ataagggatt accgtattaa
    20401 cttgtgtttg ttgcaacatg atttcagaaa cccaaatata gtatggatta gtagttcttc
    20461 gccaagggag atcacgtttt tcttggtcat accaccctaa aagtgtgcga cgaaaagaag
    20521 cgattgtctc attatcccac atattaatac cataatcttt taattcaatc atgttgttat
    20581 tgtaacacag gagtcttaaa aagagtaaaa aaagatttta ggtatttgcg aaaaagaaaa
    20641 ggaaatatta taataatatt aggtttacct catctaaatg ttgatttaga ataactgaaa
    20701 ttaacgatag aagcagacag gttttaattt ggaggggaca tgttccagaa attaaagaat
    20761 cttattgatc ggtattcgtg gttcgttttt ataatgatga ttcttgtgtt atttcttaac
    20821 tatatttatc catttgatcc ttctttgagc ggactacagt tggaatggct gatcatgacg
    20881 caagtttttc ctggattagg tatcgtatgt gccatggttg ctattatttt taaaaagttt
    20941 aacctttttg ttttggccct aattttatat tttgcacttt ggttaaaact tttccttgct
    21001 ttttcttttt tcacctattt tttaggagtc tgactaagtg ccaattttta aaactcgtga
    21061 aagaaaatgt ttgacaaaac ttagtcaagt agaaaaagtt ccttgctttt aattgactat
    21121 tagtgtacaa taagattctg tgagtatacc tcacttactc gctccaaaag tagtgagtca
    21181 ttagaccaaa aggaggaaaa tatcaatggc taaatacgaa attctttata tcattcgtcc
    21241 aaacattgaa gaagaagcta aaaacgcttt ggtagcacgc tttgactcta tcttgactga
    21301 caacggtgca actgttgttg aatcaaaaga ttgggaaaaa cgtcgtcttg catacgaaat
    21361 caacgatttc cgtgaaggac tttaccacat cgttaacctt gaagcgactg acgcagcagc
    21421 tcttaacgag tttgaccgtc tttcaaaaat caatggtgac attcttcgtc acatgatcgt
    21481 taaacttgac gcttaattcc aactaagagg gtagtttatg attaataatg tagtactagt
    21541 tggtcgtatg accaaggatg cagaacttcg ttacacacca agtcaagtag ctgtggctac
    21601 cttcacactt gctgttaacc gtacctttaa aagccaaaat ggtgaacgcg aggcagattt
    21661 cattaactgt gtgatctggc gtcaaccggc tgaaaattta gcgaactggg ctaaaaaagg
    21721 tgccttgatc ggagttacgg gtcgtattca gacacgtaac tacgaaaacc aacaaggaca
    21781 acgtgtctat gtaacagaag ttgttgcaga taatttccaa atgttggaaa gtcgtgctac
    21841 acgtgaaggt ggctcaactg gctcatttaa tggtggtttt aacaataaca cttcatcatc
    21901 aaacagttac tcagcgcctg cacaacaaac gcctaacttt ggaagagatg atagcccatt
    21961 tgggaactca aacccgatgg atatctcaga tgacgatctt ccattctagt cagaatggat
    22021 tgaacgaata cgaaattatt tgagttcggg ctaaaaattt agaacttaga atttagatca
    22081 actccctagg aaatcaagat ttcttgccag ttttctaaaa ttcagtcgtt ttttaagagc
    22141 ccttaacatc ataatttata aaggagaaaa tacatggctc aacaacgtcg tggcggattc
    22201 aaacgccgta aaaaagttga cttcatcgca gctaacaaaa ttgaatatgt tgattacaaa
    22261 gatactgagc ttcttagccg tttcgtttca gaacgtggaa aaattcttcc tcgtcgtgta
    22321 acaggaactt cagctaaaaa ccaacgtaaa gtaacaacag caatcaaacg tgctcgcgtt
    22381 atggctctta tgccttacgt aaacgaagac taataaacaa acagtccatt tcggactgtt
    22441 ttttagttga ctaggactag tctaggcgta taggtgagtt ttttctaaaa gagagaatga
    22501 tagtaaacga aaatccttgc gtagacagca aggatgaaat gattgttatt tgttaacagg
    22561 tgacatagtg ttttgaatgt tgtatttgcg ttgtaggtaa tagcgtaaag caagggaaac
    22621 accaccaata atgagcaagg caagaggagg caactgtggg ttaagagagg ttggtaaaaa
    22681 ggcagttgca gagtacaagg caatccaaat caacatggct aatgataagg ctgcgatgat
    22741 tttgaaccaa cttggacgaa ggctcttatc ttttccgaga tgacggtaaa taaagtaata
    22801 agtagcgtac atggatgctc ctccaccaaa accaagtgcc agtaaggaaa tcaatcctgt
    22861 aactgtagcg tttgtgttga agaaggtcat gatgccattt aaaagggcaa caattccgat
    22921 gaatagtaaa gaggtatcta gccacatcaa ccaaggattg gtgtttttgt cagtttctgc
    22981 tgctctttga ctagggtctt ggctaaaaga agctgcccaa gcagtcggag tgcctaatag
    23041 atttcgagcg gtgacacctt ttttctggtt ttctagaata gctggaatgg cttcttcgag
    23101 aatcagctta atatcctcat cagacttgcc gtcttggatt aatttgttcg tagcggtgtg
    23161 gataaattct tgattttttt tggtaagttc ttgtaattcc atgggaatcc cttctttata
    23221 tgggttagtt aaagtaagtc aatggtatta gctagttgta atgaaacttt ggagctagcc
    23281 gtttgacaca gagttttgtc aggtcttaaa cgtattctgc agcttgctcc actctagaac
    23341 catttttttc taataaagta aatcacaaca aaggaactaa ggagcatagc aatcaaggta
    23401 atataccaaa aagcatgttc taggccattt agtggtaacc agttgttttg gaaattcata
    23461 ccataggcgg agaaaatcac agttggtata tctaaagcca tggtcatcaa ggccagtgtt
    23521 ttcataatag tgttctggtt gttgttgata atagaagcag tggtttcagt catggcattc
    23581 aagacatttt cataaatgcc agccatctca atggcctgtt gggtttcaat caaggtatct
    23641 tctagcaaat cctcgtcctc aatgtatttc ttgagagagc ttgttgagct ggataatttc
    23701 ttgacgatgc gttcgttgaa tttcaaggaa gcctttaagt agacaatgga tttttcaagt
    23761 tccatcatgt cgataagttc ttcatttcga gtagctgctt caagctgagc ttccaaacgt
    23821 tcgctttggc gatcaatggt cctcaaggca gtcagaaaga gctcggcgtt gcgatataga
    23881 atctgaaaaa caaaacgtgt cttcatgaag gtatagaaat ttttgacccg gcggttgtga
    23941 aaatgatcga aaagggtcat gtcatgcaga caagttgtga taacagcatt ttccgttaca
    24001 ataatgccta aaggcatagt aatatagtaa cttttattgt tacgttcttc gtatatcggg
    24061 acgtctacga taattagggt ataatcatct tctacggcaa tacgtgaggt ttcttccacg
    24121 tccaaagggg cgcgcaagtc agaaatatca atgttaaatt gatcggcgat cttcatagac
    24181 tcttcttggg aagggttaac cagtttaatc caagctcctg gctcgaatgt ttctatttct
    24241 ttgaattcaa ttgccgatga aagaaacatt tgtttcatga caagccctcc tttttgatga
    24301 ttgactggta gagattgaca tcaaaccttg ataataaagc cttctcaagg attttctgaa
    24361 aaattctcaa tacttatcta ttataccaaa attcgcaaag tttggggcaa taggttatca
    24421 gaaatttttg ttataatata agaaagctcg atgaggaggg tgcctttagg cacattcaag
    24481 atgcaaaata aaataataat tcatggggca agagcccaca atttaaaaaa tattgacgtg
    24541 gaaattccac gagacaaatt agttgtcgtg acagggttat cagggtcagg aaaatctagt
    24601 ctagcctttg atactattta tgcggaaggg caacgtcgtt acgtagaaag tttatcagct
    24661 tatgctagac agttcttggg taatatggaa aaaccagatg tggattccat tgacggtctt
    24721 agccctgcta tttccattga tcaaaaaact accagtaaga acccccgctc aacggttggt
    24781 acggttacgg aaatcaatga ttatttacgt ttactatatg cgcgtgtggg cacaccttac
    24841 tgtattaatg ggcatggggc cattacagca tcttctgcag aacaaattgt agagcaggtc
    24901 ttagccctcc ctgaacggac acgcatgcag attttagcgc ctattgttcg ccggaaaaaa
    24961 ggtcaacaca aaaccatttt tgagaaaatt caaaaagatg gttatgtccg tgtgcgggtt
    25021 gacggggata tttttgacgt gacagaagtg ccggagctgt ccaaaagtaa gatgcacaac
    25081 atcgaggtgg ttattgaccg tctggttaat aaggatggta ttcgtagccg tttatttgat
    25141 tcggttgaag cagctttaag gctgggagat ggctacctta tgattgacac tatggatggc
    25201 aatgaactgc tcttttctga gcattattcc tgtcctgtct gtgggtttac ggttcctgaa
    25261 ttagaaccaa gactcttctc ttttaatgcg ccgttcggct cttgtccgac ctgtgatggg
    25321 ctaggcatta agttggaagt ggatttggac ttggttgttc ctgacccaag taagtctttg
    25381 agagagggag ctttggcgcc ttggaatcct atttcctcca attattaccc aaccatgctt
    25441 gagcaagcta tggcaagttt tggggtggat atggatacac cttttgaggc cttgactgag
    25501 gaagaaagag atttagtgct ttatggttca ggagatagag aatttcattt tcactatgtg
    25561 aatgattttg gtggtgagcg taacattgat attccttttg aaggtgttgt gactaatgtt
    25621 aatcgtcgtt atcacgaaac taatagtgat tatacgcgca atgtgatgcg tggttatatg
    25681 aacgaattga cctgtgcgac ttgtcatggt taccgtttga atgaccaagc cttgtgtgtg
    25741 catgtgggtg gcgaagaagg acctcatatt ggtcaaattt cagaattatc gattgccgac
    25801 cacttgcagt tgttggaaga attggaactc acagaaaatg aaagtaccat tgctaaaccg
    25861 attgtcaaag aaattcatga ccgcctgacc ttcttaaata atgtgggact gaattatctg
    25921 accttgtcac gggctgctgg gactttatca ggtggtgaaa gtcaacgaat caggttagcg
    25981 acacaaattg gttctaactt atcaggggtg atctatatct tggatgagcc ttctattgga
    26041 ttgcaccaaa gagacaatga ccgtttgatc gaaagcctga aaaaaatgcg tgatttgggt
    26101 aataccttaa ttgtggtaga acacgatgaa gacaccatga tgcaggcaga ctggctgatt
    26161 gatgtggggc ctggggcagg tgagttcggt ggagaaatta ttgcctctgg aacgccgaaa
    26221 caagtggcta agaataaaaa atccatcacg ggtcaatatt tgtctggtaa aaaattcatt
    26281 ccagtgccat tagagcgtcg ttctggtaat gggcgattca ttgaaataaa aggagcagcg
    26341 cagaataatc ttcaaagcct cgacgtgaga ttcccactgg gtaaatttat tgcggtcaca
    26401 ggggtatcag gctcagggaa atcaaccttg gttaatagta ttttgaaaaa ggctgtcgcc
    26461 caaaaattaa accgtaatgc tgataagcct ggtaaatatc actctatttc tggtattgaa
    26521 catattgaac gcttgattga cattgaccaa agtccgattg gaaggacgcc acggtcaaat
    26581 ccagccacct atacgggtgt ctttgatgat attcgagact tgtttgccca aaccaacgaa
    26641 gctaagattc gtggctacaa aaaaggccgt ttctctttta atgtcaaagg tgggcgttgt
    26701 gaggcttgtt caggtgatgg gattatcaaa atcgaaatgc actttttgcc agatgtttat
    26761 gtgccttgtg aggtttgcca cggcagacgg tataattctg agacgcttga ggtgcattat
    26821 aaagaaaaaa atattgctga agtacttgac atgactgtcg atgatgcctt ggtatttttc
    26881 tctgctatcc ctaaaattgc ccgaaaaatt caaaccatta aggatgttgg tttgggttat
    26941 gtgaccttgg gtcaaccagc tactactcta tctggaggag aagcgcagcg gatgaaatta
    27001 gctagtgaat tgcacaaacg ctcgacaggt aagagcttgt atatcctaga tgagccaacg
    27061 actggtttac acacagatga tattgcaagg ctattaaaag ttttggaacg ctttgtagac
    27121 gatggtaata cggttcttgt gattgagcat aatttggacg ttatcaaatc tgctgaccat
    27181 attattgact taggaccaga aggtggtgtc ggaggtggtc agattgttgc cacggggaca
    27241 ccagaagagg tagctcaagt taaagaaagc tatactggtc attatttgaa agtaaaacta
    27301 caacaatagg attaggagtt cgtttgagct cctttttaat cttttaaatg ctctcaaatc
    27361 atggtaaact taaagtagtt ttttcatgct acaatattct caaaagttac caaaggagtt
    27421 cgcgatgtca ggatttttag agcaacgatt aggtcactgc ctaaggcaga tggcagagaa
    27481 ggggctagag gctcttctag tcacccattt aaccaatagt tattacttga caggtttttc
    27541 tggaactgca gcaactgttt tgataacggc caaacgtcgt gttttgatca cagattcacg
    27601 ttataccttg cttgctaaag ctagtgttga gggatttgat attatcgaaa gccgcacgcc
    27661 gcttaaggtt gtggcagaat tgttagaggc tgatcaaata gattgccttg gttttgagga
    27721 ccaggtatcg ttttcttttt atcaggccat gcaagcagaa ctgtcaggaa taaccttgct
    27781 tgctcagtca ggttttgtgg agcatttacg ttttattaag gatacttctg aaattgatac
    27841 cattgctaaa gcgtgctcga tctcagacaa agcatttgaa gatgctcttg attttattaa
    27901 accagggaca accactgaac gtgacctggc taatttttta gattttcgta tgcgtcagta
    27961 tggtgccagc ggcacatcat ttgatatcat tgtagcttca ggctatcgct ctgccatgcc
    28021 tcatggacgc gccagtgaca aggttatcca gaataaagag agcttgacca tggactttgg
    28081 gtgttactac aatcactatg ttagtgatat gacgaggacc attcatattg gccaagttac
    28141 tgatgaagaa cgtgagattt atgctcttgt tcttgctgct aataaggctt taattgctaa
    28201 agctatcgct ggcatgactt atagtgactt tgacggtatt ccgcgccaac tcatcactga
    28261 ggcgggttat ggcagtcact tcacacatgg cattggtcat ggtatcgggc ttgatatcca
    28321 cgagaatcca ttttttggga aatctgagca acttctccaa gctggaatgg tggtcacaga
    28381 cgagccaggt atctatttgg ataacaaata tggtgtccgt atcgaagacg acttggttat
    28441 cacaaaaact ggttgtcaag tcttgacctt ggcacccaaa gaattaattg tattgtaaaa
    28501 gaatggttca ggaaaggatt aggggagaag caatgacaaa tcgtttatca tggcaagact
    28561 attttatggc caatgcagag ctgatttcca agcgctctac ctgtgatcgc gcctttgtgg
    28621 gagctgtttt ggtaaaggat aaccgcatta ttgccacagg ttacaatggc ggagtttctg
    28681 ctacggataa ctgcaacgaa gcgggtcatt atatggaaga cggacattgt attcgcactg
    28741 ttcatgctga aatgaatgct ttgattcaat gtgcgaaaga aggaatttca acggacggaa
    28801 ctgaaattta cgtgactcat tttccttgta ttaactgcac taaggcgctt ttgcaggcgg
    28861 ggattacaaa gattacctat aaagcacatt accgtccgca cccctttgcc attgaactga
    28921 tggagaaaaa aggggttgcc tatgtgcaac atgatgtgcc tcaaattgtt ttaggaggcg
    28981 ataaggaaca atagaaagtg tacctaaaag gacggtcata aaagatttga gccaaggcca
    29041 cttttgtggt aaaatgaagt gataaatatt ttagtaagag gtaataataa tgattgaagc
    29101 aagtaagctt aaagcaggta tgacatttga agcagaagga aaattaatcc gtgtccttga
    29161 agctagccac cacaaaccag gtaaaggaaa cactatcatg cgtatgaaac tacgtgatgt
    29221 gcgtacaggt tctacttttg acacaactta ccgcccagat gaaaaatttg agcaagccat
    29281 cattgaaact gtcccagcac aatacctata caaaatggat gacactgctt acttcatgaa
    29341 cactgatact tatgatcagt acgaaattcc agttgctaac gttgagcaag aattgcttta
    29401 cattcttgaa aactcagacg tgaaaatcca attttatgga agtgaagtga ttggggtaac
    29461 ggttccaaca actgttgaat tgaccgttgc ggaaacacaa ccatctatta aaggagcgac
    29521 ggtgacgggt tcagggaaac ctgcaactct tgagacagga cttgttgtta acgttccaga
    29581 ctttatcgaa gctggccaaa aactaatcat taacactgca gaaggtactt acgtttctcg
    29641 tgcttaatcc tttgtcaaaa cagattagtc taaaaaagag aaaggaaatg ctatgacaac
    29701 tgaatatatc ggtgaaatcg tcatttcacc acgagtgctt gaggttatta cggggattgc
    29761 tacgacacaa gttgagggtg tccactcact ccataacaag aaaatggcag atagctttaa
    29821 caaagcaagt cttggtaaag gggtttatct ccagacagaa gaagatggaa gtgttacagc
    29881 agatatttat gtttacttgc aatatggagt taaggtcccg actgtatcta tgaatattca
    29941 aaaaacagtc aaatctgctg tgtacgatat ggcagaagtg cctatttctg cagttaatat
    30001 ccatgttgaa ggtattgttg cggaaaagac accaaaacca gatttgaagt cactttttga
    30061 tgaggatttc ttggatgact aacagctttc aaaattcaag aagagatttg cgtgaacgtg
    30121 cctttcaggc tttatttaac atagagatgg gagctgagtt attggcagct tctcagtttg
    30181 cctatggtta tgataaagta acaggggagg acgctcaagt attggagctg ccgatttttt
    30241 tattaagtct tgtgacgggt gtcaataatc ataaagaaga gttggataac ttgatatcaa
    30301 ctcatttgaa aaaaggttgg tcgttagaac gcttgacact aacagacaaa acgttattac
    30361 gactaggtct ttttgaaatc aagtattttg acgaaacacc agaccgtgtt gcactaaacg
    30421 aaatcattga agtagctaaa aaatattctg atgaaacctc tgctaaattt attaatggct
    30481 tacttagcca atatgtctcg gaagctcctt cagcaaataa atcataataa gtcggagcaa
    30541 tcttaggatt gtatccgatt ttttcaattt atgtacgaat attaaaagac aaacaagcat
    30601 actctcgcat ctagaaagag tatgcttgtt tgtcttttag agtaattagt gattggttat
    30661 gttttgttta aatacttgct cctggaagta gcgtaatcgg taaaatatag tctttacttg
    30721 ttttttcttt tttaattttt ttgatgagta tttcgacact caatttagca atctcatcaa
    30781 taggttgacg aatggtcgct aattgaggaa catattgctg gataaatgtc gttccatcat
    30841 aacctatgac tttcatatcc tctggaatag taatgtgaag ttgtttggct actttcataa
    30901 tgaggatagc agttaaatca tcagaaacga aaaggccatc aggttttcgg gttgccaaaa
    30961 tggatttaat ttccatttcg cgtctaacag gagataggtt atttggtaat ttgatgattt
    31021 ctgcacttcg tttgagctgg tagttaaacc ctagctggcg caaccctgtg ggcgaatcag
    31081 aattgtcatt acctgtaatc atgacaatgt tttgacaacc gtgtttttgc aaagtttgtg
    31141 cggctaattt accaccttca aaattatctg aagaaataac cggaatattt ggcgccagat
    31201 tacggtcaaa agctacaata ggagcttcga cacgctcata atcctcaatt cccaaattat
    31261 gactggaaga aataatgccg tccacttgat tggccgcaag catttctaag tattcgcgtt
    31321 ctttgacggg attatgttca ctattgcaaa taatagtttt gtagccctgt ttaaacaatt
    31381 caatttccaa atgttcaatt aattcagcgt aaaagatatt gctaatgttg gggaagataa
    31441 gcccaatgag ttgggttgat ttaccttgca aacttctagc gagattgtta ggcttgtagc
    31501 ccaattcacg catggcctta ttcaccttat tcactgtttt ttgggaaagg tagccttttt
    31561 tgttgattac tcgcgaaaca gttgttggac tgacacccgc caaggctgcc acgtctgtta
    31621 attttgcgac cacttgccta atacctcatt tcataataat gtccaaaagc ttgtcctttt
    31681 actacttgga tacctgtttg tccttgggtt gggaaaacac gactagttag gactttttgc
    31741 ccttggttaa taaagatttc aagaatggaa ttgtccacat agacattaag tgtaacatgg
    31801 ccttgtggga tttggcaaga gcgcaccgta ccatagtctt gggcatattg aacccctgcc
    31861 cgacttcggt caatgcttaa ctgtcctttt gttgtatcca ctttcaatct taggccattc
    31921 cctttttggt cagtaaatag gagcaggtca agcttttttt ggcctggaat cgtcaattcc
    31981 aattcataac agttagaact gtctatttta ttatgaaata attctgctgg ccctcgaaga
    32041 ttttgtaggg ctgaaacagg tgtttgatag agaattccgt ctttaatcct taattctttc
    32101 actaaactaa gagctccttg atagtcataa cgatctgtcg gataatcaat atctggtaac
    32161 ccaatccaag acacggctag aaccctgcca tctggactac taaaggcttg agtggcataa
    32221 gcttcaaaac caaagtcaag attttgaagg acccccccac ccagcaattg gccagtttct
    32281 ggattgaagc tttcaaaaat tttataggta ttggggtaga tattttgata atctaaatca
    32341 gctttagcca gtccttgtgg gctgaaaatc aaaacaggct tgtcatcgac aaacacaagg
    32401 ttggggcatt caatcatgta ttccgtacca gaatcgtcaa agtccaaatc ggctataaat
    32461 cgccagttgt ccacatgatt gtccacagct ttgtaaagtt tgatttttcc tttgccatct
    32521 aaaccttggg cgccaataat agcatagaat tgaccttgat aagaaaaaag ctgaggatcc
    32581 cgaaaatgtt ctgtaacatc atcgggctgt tcaatcaaaa cctgtggtat tttggaaata
    32641 tttccttgtt tatccatcca tgcgccaacc tgtaatggtg ttctgaccca attggcatcg
    32701 cgaacattac cagtgtaaaa gaggaagagc ttgtcatcaa tagcataggc agaacctgag
    32761 taagccccat gactatcgtg agcgtggtct ggcagaaggc ggctccttgt ttccgtaaaa
    32821 tggacgaggt ctgtggatgt catgtgtacc cattgtttca gaccatgcgc agccccataa
    32881 ggccagtttt gataaaaaag atggtagtga ccgttgaaat aagaaaaccc attggggtca
    32941 ttgagtaatc ctgtcttagg ttcgacatga aattggctgt gccatgggga ttgggccatt
    33001 ttttcagtaa tagcttggta gtcttttgag ctccactctt tgtaagggcg gtagcgaata
    33061 gcttgtggta gatccatcaa aatctccttg ttttatagtc gtcttcaaga atatactaaa
    33121 cgttttctgt ttaaaaatca agaatttaaa tcatataaaa taaaaatatg ttaaacgctt
    33181 gacataaaaa atattagtga taaaatgata gtcaagaagt gaagcggatt cactagcgtt
    33241 ggtcatgcac attcttagcc acaaagctgg cagaatggat gagggactaa caccaaatga
    33301 aaaaggagac ttttaggcat ggataatcgt cagattgcag ctgaagtgat tgaggcttta
    33361 ggtggccgag aaaatgtgag aagtgttgcc cactgtgcaa ctcgccttcg cgtgatggtt
    33421 tatgatgaag gaaagattga taaggaaaaa gcagaagcta ttgacaaggt taaaggagct
    33481 ttctttaact ctggtcaata tcaaatgatt tttggaactg gtaccgtcaa taacatttat
    33541 gacgaagtcg ttgctcttgg tttaccaaca tcatcaacca gtgagcaaaa agcagaagca
    33601 ggcaaacaag gcaatatctt ccaacgggcg attcgtacgt ttggggatgt ctttgttccc
    33661 attattccag ctattgtagc aacgggtctc tttatggggg ttcgtggttt ggtgacccag
    33721 ccagctatta tggatttatt tggggtgcat gagtacgggg aaaattttct catgtacact
    33781 cgtattctaa cggatacagc ctttgtctac ttgccagctt tggtggcttg gtcagccttt
    33841 agggtctttg ggggtaatcc tattatcggt attgttttag gcttgatgtt ggtgtccaat
    33901 gaattgccaa acgcctgggt agttgcctct ggtggagatg ttaagccgct aaccttcttt
    33961 ggatttgttc ctgttgttgg ttatcaaggt accgttttac ccgccttctt tgttggtctg
    34021 gtaggagcta agttggagaa atggttgcac aaaaaggttc cagaagcttt agacttattg
    34081 gtcacaccgt ttttaacgtt tgctattatg agtaccttgg gactatttgt gattggacca
    34141 gttttccatt ctcttgaaaa ccttgttctg gctgggacac aggccgtctt gcatttgccg
    34201 tttggcattg ctggtcttat tgttggtggt atccaacagt tgatcgtcgt tactggtatt
    34261 caccatatct ttaacttcct agaagcgcag ctgattgcca ataccggaaa agatcctttc
    34321 aatgcttacc taacagcagc aacagctgct caagctggag ctaccttagc tgttgctgtt
    34381 aaaaccaaat caacaaaact aaaaggtctg gcctttccat caaccttgtc agctctttta
    34441 gggattactg aaccagctat ttttggggtc aacctccgtt atccaaaagt ctttgtttca
    34501 ggtcttattg gtggtgcctt aggtggttgg gtcgctgggc tctttggcat tgcaggaact
    34561 ggttttggga ttacggtttt accaggaact ctcctttact tgaatggcca attattacaa
    34621 taccttgtga ctatgcttgt cggtcttggg gtagcctttg caattgctta tacttggggt
    34681 tatcaagata gagaaaccct tccgttacca gctgtcgaag ttgatcaaac ggctgatcag
    34741 ccagccttag cagaggaaac tttatatagt cctttgaatg gaacagttgt agatttagca
    34801 gctgtttcag accctgtttt ttcatcaggt gctatgggtc aaggcttagc aattaagcca
    34861 gaagataata ccctatactc accagttgat ggtaaagttg aaattgtctt tgaaacaggt
    34921 catgcctatg ctataacgtc aagtcaagga gcagaagtgt tacttcatat tggtattgat
    34981 accgtgtcga tggcaggaga tggctttgaa tctcttgtag cagtaggtca ggcagttaaa
    35041 aaaggtgatc ttcttggaca ctttgatcct agcaagattg cagaagctgg actagatgac
    35101 accaccatga tgattgtgac caatagcgct gactatcaaa gcgtggacat tcttgctcaa
    35161 ggacatgttt tgattggtga tcaagtggca cttatcaaat aaggacaaaa gagctaacta
    35221 agcagctctt ttgtcctgcc atgaatggca ggttggcaaa aaaatgagaa aagcctaaaa
    35281 accttaaatt tgtgttattt tacctatact ttgtaccttg ttttttttaa taaagtgttg
    35341 ttatacttaa ggcgaactat aggaatgcgc ttacatagat ggtatatcaa ctgggaagcc
    35401 atgacttagt accaaaaata aggagtgtcc aaatggataa acatttgttg gtaaaaagaa
    35461 cactagggtg tgtttgtgct gcaacgttga tgggagctgc cttagcgacc caccatgatt
    35521 cactcaatac tgtaaaagcg gaggagaaga ctgttcaggt tcagaaagaa ttatcttcta
    35581 tagatagctt gcattatctg tcagagaata gcaaaaaaga atttaaagaa gaactctcaa
    35641 aagcggggca agaatctcaa aaggtcaaag agatattagc aaaagctcag caggcagata
    35701 aacaagctca agaacttgcc aaaatgaaaa ttcctgagaa aataccgatg aaaccgttac
    35761 atggtcctct ctacggtggt tactttagaa cttggcatga caaaacatca gatccaacag
    35821 aaaaagacaa agttaactcg atgggagagc ttcctaaaga agtagatcta gcctttattt
    35881 tccacgattg gacaaaagat tatagccttt tttggaaaga attggccacc aaacatgtgc
    35941 caaagttaaa caagcaaggg acacgtgtca ttcgtaccat tccatggcgt ttcctagctg
    36001 gaggtgataa cagtggtatt gcagaagata ccagtaaata cccaaataca ccagagggaa
    36061 ataaagcttt agccaaagct attgttgatg aatatgttta taagtacaac cttgatggct
    36121 tagatgtgga tgttgaacat gatagtattc caaaagtaaa tggagaagct agtgacgaaa
    36181 atctcaagcg atctattgat gtttttgaag aaattgggaa attaattgga ccaaaaggtg
    36241 ttgataaatc gcggttattt attatggata gcacctatat ggctgataaa aacccattga
    36301 ttgagcgagg agcttcttat attaatttat tactggtaca ggtctatggt tcacaaggag
    36361 agaaaggtgt ctttcaaaat gataccaaat tagtcaccga tacccctgaa gaacgatggc
    36421 aaggttatag caagtatatt cgtcctgaac aatacatgat tggtttttct ttctatgagg
    36481 aaaatgctca agaagggaat ctttggtatg atattaattc tcgcaaggac gaggacacgg
    36541 caaatggaat taacactgac ataactggaa cgcgtgccga acggtatgca aggtggcaac
    36601 ctaagacagg tggggctaag ggaggtatct tctcctacgc tattgaccga gatggtgtag
    36661 ctcatcaacc taaaaaatat gctaaacaga aagagtttaa ggacgcaact gataacatct
    36721 tccactcaga ttatagtgtc tccaaggcat taaagacagt tatgctaaaa gataagtcgt
    36781 atgatctgat tgatgagaaa gatttcccag ataaggcttt gcgagaagct gtgatggcgc
    36841 aggttggaac cagaaaaggt gatttggaac gtttcaatgg cacattacga ttggataatc
    36901 cagcgattca aagtttagaa ggtctaaata aatttaaaaa attagctcaa ttagacttga
    36961 ttggcttatc tcgcattaca aagctcgacc aatctgtttt acccgctaat atgaagccag
    37021 gcaaagatac cttggaaaca gttcttgaaa cctataaaaa ggataacaaa gaagaacctg
    37081 ctactatccc accagtatct ttgaaggttt ctggtttaac tggtctgaaa gaattagatt
    37141 tgtcaggttt tgaccgtgaa accttggctg gtcttgatgc cgctactcta acgtctttag
    37201 aaaaagttga tatttctggc aacaaacttg atttggctcc aggaacagaa aatcgacaaa
    37261 tttttgatac tatgctatca actgtcagca atcatgttgg aagcaataaa caaacagtga
    37321 aatttgacaa gcaaaaacca actgggcatt acccagatac ctatgggaaa actagtctgc
    37381 gcttaccagt ggcaaatgaa aaagttgatt tgcaaagcca gcttttgttt gggactgtga
    37441 cgaatcaagg aaccctaatc aatagcgaag cagactataa ggcttaccaa aatcagcaga
    37501 ttgctggacg tagctttgtt gattcaaact atcattacaa taactttaaa gtttcttatg
    37561 agaactatac cgttaaagta actgattcca cattgggaac cactactgac aaaacgctag
    37621 caactgataa agaagagacc tataaggttg acttctttag cccagcagat aagacaaaag
    37681 ctgttcatac tgctaaagtg attgttggtg acgaaaaaac catgatggtt aatttggcag
    37741 aaggcgcaac agttattgga ggaagtgctg ataaggaaat gtctagaaac gtatttgatg
    37801 gttctttagg aggagaccga tctaatctac taggcatgtc aggaccaaaa tctacaatta
    37861 tttttaattt aaaagaatct ggaatagtaa aacactggag attttttaat gatagagcac
    37921 ggtcacctca gaatccggcc gaagacatcc aagaaattaa actccaagtc tttgactcag
    37981 aaaaatacga tattgaaaca ctattaaaga cgcctaatca atttgataaa gacgattact
    38041 ggattacggt tgatagttat aaatcaaaat ccgaaactgt taataattat gatcaagttt
    38101 tgaaaatgaa ttcagccaaa tactggagag taaccgtaga tactaaggga ggaaattata
    38161 gttggccatc actccctgaa ctccaaattt taggttatcc gttacctaac gccgacacta
    38221 tcatgaaaac agtaactatt gctaaaggtc tatctcaaca aaaagataag ttttctcaaa
    38281 agatgcttga tgagttaaaa ataaaagaga tggctttaga aacttctttg aacagtaaga
    38341 tttttgatgt aactgctatt aatgctaatg ctggagtttt gaaagattgt attgaaaaaa
    38401 ggcagctgct aaaaaaataa acaaagcaac tttcttagat agcaacattc agattaaatt
    38461 aacaaaatgt gactatgata aaggtttgct ggaattgatt aaccaaaaga ctaaaaatct
    38521 gagatgaata gtcccagatt tttagtcttt tataggtttt gatgacataa agctaaataa
    38581 tcgttagact accagaaagt ggcgcttgtc cgtgagacat ggctgtcttg ataagagcct
    38641 gtgtcatcat aaagataaaa aagaacaaga aaaaggagaa aacatgggaa aattatatgg
    38701 aagcattgaa gctggtggga caaagtttgt ctgtgccgtg ggtgatgagg agtttacagt
    38761 ggtggataaa acacaatttc cgaccaccac accagaagaa accatcgcgc aaaccatcgc
    38821 atattttaaa acctttgagg ccgatttggc tggaatggcg attggttctt ttggaccaat
    38881 tgatattgat ccgtcgtcag agacttacgg ttacatcaca accactccta agtcaggctg
    38941 ggctaatgtg gatttgttag gacaactttc ggctgctttt aagattcctt ttgatgtgac
    39001 aacagatgtt aatagctctg cttatggtga ggtgcttgct cgtcctggtg tggaaagttt
    39061 ggtctattac accataggta ctggtattgg ggcgggagct attcaacatg gccactttat
    39121 tgggggatta ggtcatacag aagcgggtca tacctatgtc atgcctcatc ctgatgatat
    39181 ggccaaggga tttttaggtg tttgcccttt tcataaaggg tgtctggaag ggatggcagc
    39241 aggtccaagt attgaggcca gaacgggagt gagaggtgaa cgacttgatc aggaagctga
    39301 tgtttgggac attcaagcct tctacattgc ccaagcagcg ctgcaagcaa ctatgcttta
    39361 ccgtcctcaa gtgattgttt ttggcggagg tgtcatggcg caagaacata tggtgttgcg
    39421 cgtgcatgat aaattcacta cccttttaag cggttatttg ccagtccctg atttgacaga
    39481 ctacattgtt actccagctg ttgctgataa tggctccgct actctgggaa attttgcttt
    39541 ggccaagttg gctgcacaag gaaaataggg tctgtttttg cctgcttgtc tagtaaaaga
    39601 aagcgcagtc agctagaaca tgctataata aacagagatt aacatcagaa gcgaggaata
    39661 ttatgtcaga accattattt ctaaaatcaa ccatgcacga caggatttgg ggtggcacca
    39721 agttgagaga tgtctttgct tacaacatcc caagtgacac cacaggtgag tattgggcta
    39781 tttctgctca tcctaatggt gtttcgacag ttactaatgg acgttatcaa ggacaacctt
    39841 taaacacatt atatgcgcaa gagccggcct tgtttgggaa tccaaaggaa gaagtttttc
    39901 cactgttaac taagatttta gatgctaatg actggctaag tgtccaagtt catcctgatg
    39961 atgcttatgg acgtgaacat gaaggagaat taggcaagac agaatgctgg tatattattt
    40021 ctgccgaaga gggttctgaa attgtatacg gccatcaggc caagtccaaa gaagaccttc
    40081 gtgccatgat tgaagcaggt gcttgggatg atttgctgac tcgtgtccca gtcaaagcag
    40141 gcgatttttt ctatgttcca agtggcacca tgcatgcgat cggcaaggga attcttattt
    40201 tagaaaccca gcagtcatct gataccactt atcgggttta tgattttgac cgtaaggacg
    40261 ttaatggtaa tcttcgtgac cttcatattg aaaagtctat tgatgtctta acgattggta
    40321 agccagaaaa tagcgtgcca gccactatgg ttttggataa tatggtagca acaactttag
    40381 tctcaacgcc atttttcact gtttacaaat gggtgactag tcaaatggtt gacatgaaac
    40441 aagctgcgcc ttatctttta gtgagtgttt taaaaggtca aggaaaactc tatgtggacc
    40501 agaaggctta tgagctagaa aaaggcatgc actttatctt gcctaacgat gttaaatcgt
    40561 ggtcatttga tggtcaatta gaaatgattg tgagtcaccc caatcaactc gtttaatacc
    40621 atggtatcaa tatgagaaaa aaggataaaa ttatgactct ctgtttctct gataacttta
//
LOCUS       DAUGHY010000014        36238 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.014, whole genome shotgun sequence.
ACCESSION   DAUGHY010000014 DAUGHY010000000
VERSION     DAUGHY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36238
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.014"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            351..1100
                     /gene="truA"
                     /locus_tag="VH752_000666"
     CDS             351..1100
                     /gene="truA"
                     /locus_tag="VH752_000666"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEQ8289039.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
     gene            1090..1854
                     /locus_tag="VH752_000667"
                     /pseudo
     CDS             1090..1854
                     /locus_tag="VH752_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
     gene            1844..2314
                     /locus_tag="VH752_000668"
     CDS             1844..2314
                     /locus_tag="VH752_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ8289040.1"
                     /translation="MQNSKIRQMSLTGILTALVVVLGRFVMLPTPTGFLTLLDAGIYA
                     VSFSFGSAQGAIVGGLSGFLIDLVAGYPQWMFHSLIAHSVQGYFAGWRGRKRWLGVVI
                     GSFIMIFWYFLGSLMLGYGLSGSLAGIWGNVMQNTLGLFVGFIIFKAILRQKKR"
     gene            2328..2888
                     /locus_tag="VH752_000669"
     CDS             2328..2888
                     /locus_tag="VH752_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEQ8289041.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVGQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERHVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQFMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(2953..3798)
                     /locus_tag="VH752_000670"
     CDS             complement(2953..3798)
                     /locus_tag="VH752_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEQ8289042.1"
                     /translation="MTFILHYLEQSHIENIGLTIFKKLVSLIILLLFFAILKRVTNYL
                     FEKTINKSFAYSRQSEARKKTLSKLTHNILNYLLYFLLIYWILSLFGIPVSSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILFENQFEVGDNVTISDIEGSVFGVGIRTTQIRGFD
                     GTLHFIPNRSITVVSNKSRGNMRALIEIPLYSTVNLSQVTRIIDEVNQKELPNHPQIV
                     GKPNILGPQNNSNGQFTFRIAIFTENGEQFKIYHTFYRLYQEALLKEGIQLPTAISYS
                     ITSKS"
     gene            4119..5402
                     /locus_tag="VH752_000671"
     CDS             4119..5402
                     /locus_tag="VH752_000671"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEQ8289043.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLGLGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
     gene            5614..6189
                     /locus_tag="VH752_000672"
     CDS             5614..6189
                     /locus_tag="VH752_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEQ8289044.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL"
     gene            6446..8050
                     /locus_tag="VH752_000673"
     CDS             6446..8050
                     /locus_tag="VH752_000673"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEQ8289045.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
     gene            8218..9144
                     /locus_tag="VH752_000674"
     CDS             8218..9144
                     /locus_tag="VH752_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ8289046.1"
                     /translation="MKTIRIAKYLGILFLLITLISVGASFYFFHVAQIREEKSFINNK
                     KRSTNNPLYPAEQSFDALPYEKRQLTNRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKE
                     DMKPYAMLFHDLGYNVLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQLIKENPESQI
                     TLFGLSMGAATVMMASGERLPAQVTSLIEDCGYTSVWDELKFQAKAMYNLPAFPLLYE
                     VSALSKIRAGFSYGEASSVKQLAKNKRPTLFIHGDKDDFVPTKMVYDNYKATKGPKEI
                     LIVKGAKHAKSFETNPEQYQKKIAAFLKKVEK"
     gene            9198..9283
                     /locus_tag="VH752_000675"
     tRNA            9198..9283
                     /locus_tag="VH752_000675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9232..9234,aa:Leu,seq:aag)
     gene            9490..10371
                     /locus_tag="VH752_000676"
     CDS             9490..10371
                     /locus_tag="VH752_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEQ8289047.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQTLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            10753..10941
                     /locus_tag="VH752_000677"
     CDS             10753..10941
                     /locus_tag="VH752_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEQ8289048.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
     gene            11093..11458
                     /locus_tag="VH752_000678"
     CDS             11093..11458
                     /locus_tag="VH752_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ8289049.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            11458..13122
                     /locus_tag="VH752_000679"
     CDS             11458..13122
                     /locus_tag="VH752_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEQ8289050.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSGLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKTKA
                     EVKDFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSNPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            13359..14249
                     /locus_tag="VH752_000680"
     CDS             13359..14249
                     /locus_tag="VH752_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEQ8289051.1"
                     /translation="MLGPFIFIAFGVIVILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHVRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNVATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFAAKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
     gene            14478..15332
                     /locus_tag="VH752_000681"
     CDS             14478..15332
                     /locus_tag="VH752_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEQ8289052.1"
                     /translation="MKSKKVVSVISLTLSLFLVIGCAKVDNNKSVNRKPATKQTYNSY
                     SDDQLRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDE
                     TVLDNSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISD
                     RSTTQVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLL
                     DFADFSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEER
                     RKSLKSFK"
     gene            15525..16721
                     /locus_tag="VH752_000682"
     CDS             15525..16721
                     /locus_tag="VH752_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEQ8289053.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAEFDQALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            16984..17535
                     /locus_tag="VH752_000683"
     CDS             16984..17535
                     /locus_tag="VH752_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ8289054.1"
                     /translation="MKTLRKLLSNYKFDIKKFKLGMRTLKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLFSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMVNVACNNKSGIIGAVAALLIITLSIPTGQTFIYVTSRVFETFCGVFVA
                     ILVNTDVELIKNKWFNKKHSDVR"
     gene            17602..17973
                     /locus_tag="VH752_000684"
     CDS             17602..17973
                     /locus_tag="VH752_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ8289055.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
     gene            18011..19357
                     /gene="glnA"
                     /locus_tag="VH752_000685"
     CDS             18011..19357
                     /gene="glnA"
                     /locus_tag="VH752_000685"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEQ8289056.1"
                     /translation="MAITVADIRREVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     KPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
                     APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIAREHGLYATFMAKPKFGIAGSGMHCNMSLFDNQGNNAFYDETDKRGMQLSEDAY
                     YFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPVEANIYTMTMEERNEAGIIDL
                     PSTLHNALKALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFVSQWEIDHYIHNY"
     gene            complement(19585..21267)
                     /locus_tag="VH752_000686"
     CDS             complement(19585..21267)
                     /locus_tag="VH752_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ8289057.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEALIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(21269..21499)
                     /locus_tag="VH752_000687"
     CDS             complement(21269..21499)
                     /locus_tag="VH752_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEQ8289058.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLDYEKETGVFELTEF"
     gene            21782..22480
                     /gene="tsaB"
                     /locus_tag="VH752_000688"
     CDS             21782..22480
                     /gene="tsaB"
                     /locus_tag="VH752_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEQ8289059.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHAPLEVIIEQLVEEGQLIFVGE
                     TAPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSHYVKRI"
     gene            22452..22907
                     /gene="rimI"
                     /locus_tag="VH752_000689"
     CDS             22452..22907
                     /gene="rimI"
                     /locus_tag="VH752_000689"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEQ8289060.1"
                     /translation="MIVTTLSESNMKTVEEQAKKIYQLLEMVYGTSPWTLEQVLIDIR
                     RDQTDYFLLYDHDKLLGFLAIQDLAGEVEMTQIAILPSHQGLGLASQLMTHLDSIESD
                     IFLEVRESNHRAQGLYQKFGFKLIGKRPDYYRNPIETALLMKREGKHDR"
     gene            22897..23925
                     /gene="tsaD"
                     /locus_tag="VH752_000690"
     CDS             22897..23925
                     /gene="tsaD"
                     /locus_tag="VH752_000690"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEQ8289061.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMITEDHLEFSFSGLKSA
                     FINLHHNAKQKGDELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQ
                     GLRDRLAQEITHIEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQ
                     FPDSFVIN"
     gene            complement(24096..24365)
                     /gene="rpsN"
                     /locus_tag="VH752_000691"
     CDS             complement(24096..24365)
                     /gene="rpsN"
                     /locus_tag="VH752_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEQ8289062.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
     gene            complement(24585..25343)
                     /locus_tag="VH752_000692"
     CDS             complement(24585..25343)
                     /locus_tag="VH752_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8289063.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     DITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSFIPIEVISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSCQSDTH"
     gene            25583..26362
                     /gene="udp"
                     /locus_tag="VH752_000693"
     CDS             25583..26362
                     /gene="udp"
                     /locus_tag="VH752_000693"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEQ8289064.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            26383..27585
                     /locus_tag="VH752_000694"
     CDS             26383..27585
                     /locus_tag="VH752_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEQ8289065.1"
                     /translation="MQFIYSIIGILLILGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRSTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            27615..28286
                     /gene="deoC"
                     /locus_tag="VH752_000695"
     CDS             27615..28286
                     /gene="deoC"
                     /locus_tag="VH752_000695"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEQ8289066.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECEDAIQNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAKCQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAETFIALGASRLGTSRIIKIVKNEAI
                     KTDSY"
     gene            complement(28381..29550)
                     /locus_tag="VH752_000696"
     CDS             complement(28381..29550)
                     /locus_tag="VH752_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ8289067.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYGYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDDLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVKLKEEDHGR
                     MFPTTDKSRTIIDALEKKIKALGGKVLTSTEVVSVKKQDDLFYLKSADQTFSCHKLIV
                     TTGGKAYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTLSY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVADFLSEDYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
     gene            29755..30285
                     /locus_tag="VH752_000697"
     CDS             29755..30285
                     /locus_tag="VH752_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEQ8289068.1"
                     /translation="MGIEKTVSELADILGVSRQAVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFFTRLFAK"
     gene            30334..30921
                     /locus_tag="VH752_000698"
     CDS             30334..30921
                     /locus_tag="VH752_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEQ8289069.1"
                     /translation="MKHLDTYIAFDLEFNTVNDVSHVIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSNKIAAAPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIDLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAA
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            31073..31810
                     /locus_tag="VH752_000699"
     CDS             31073..31810
                     /locus_tag="VH752_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ8289070.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTAGGRRLYT
                     DSDLEQLRMICFLRDLGFSIEQIRKVLAEENAAQVLELLLVDHIATAKEDLAAKEQQV
                     DIAVKILDRLRKQDPQSLDFLMDISLSMKNQKAWKKLQWKMGISIFLAIVSYVGLILL
                     SNYLQKFWLVYVAMGIFLPGFSWLVIYFRKQFIYLCPHCHQKFEPSFNEFALAGHTPR
                     TRKLTCSHCHQKSYCLELAKEDTVSRG"
     gene            complement(31867..32166)
                     /locus_tag="VH752_000700"
     CDS             complement(31867..32166)
                     /locus_tag="VH752_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEQ8289071.1"
                     /translation="MMNMQNMMKQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDEATKKTLGAFAGKLPF"
     gene            complement(32359..32733)
                     /locus_tag="VH752_000701"
     CDS             complement(32359..32733)
                     /locus_tag="VH752_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8289072.1"
                     /translation="MSAKKTFFASNLKYLRLKKNMEQLELANLLGRKSSSSISEWEKG
                     KYTPKSGLLSDIAAIFDVSLTALMEEDLTLESKGVSYQLPQRELAMISEQLSENNYTK
                     WVDFAQILLEQQAKEDRELDNH"
     gene            32903..33124
                     /locus_tag="VH752_000702"
     CDS             32903..33124
                     /locus_tag="VH752_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8289073.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
     gene            complement(33204..35486)
                     /locus_tag="VH752_000703"
     CDS             complement(33204..35486)
                     /locus_tag="VH752_000703"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEQ8289074.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKANLESFLRKL
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKVNIIRPQSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRRQITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGDYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDHYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYTHGLQDWNVKPRQVYEIFNALPSTINKHLFLHQGEHVYMHNWQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFGTSTIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRASKAALFKGKANQALIDILLEEDLPINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSREPLKELPFREDSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGDTLRVILYTTDFEHTVRDNS
                     NYALTIDLSQSQLIVPIASN"
     gene            complement(35508..36167)
                     /locus_tag="VH752_000704"
     CDS             complement(35508..36167)
                     /locus_tag="VH752_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ8289075.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
BASE COUNT        11467 a         6033 c         7598 g        11140 t
ORIGIN      
        1 gactatgtgg cttatcaaga ggtaatagct agcttagaga accaattaaa ttcgcaagag
       61 cgtatgcagt ttcaagcact tatcaggggc gaacgtttca agggaagacg tgctctactt
      121 aggaagatca gtccctattt taaggaattt gcacagcagt tgtaagcaag acctataagg
      181 aattgggggg taaatctatg aaaaaaggag agagtttgga ggaaatagct ttcttatagc
      241 ttttaaatcg ctagtggctt agagtaagct tcaatacgtc tttcatttgt ttgggtacag
      301 tttgattaaa aatagtttct cttacgttta agaaatgtta aaatagtaac atggtaagat
      361 acaaagcaac gatttcctat gatgggactc ttttttcggg ttttcaaaga caaagacatt
      421 tacgtacggt tcaagaagag attgaaaaaa cactctataa attaaataac ggtacaaaaa
      481 ttataattca tggtgcagga cgtacggatg caggagtaca tgcttatgga caggtgatcc
      541 attttgattt gcctcaagaa caagaagttg aaaaattacg atttgctctt gatacacaga
      601 ctcctgaaga tattgatgtt gttaatatag aaaaagtggc tgatgatttt cattgtcgtt
      661 atcagaagca tttaaaaact tatgaatttt tagttgataa tggtcgacct aaaaatccga
      721 tgatgcgaca ctataccact cattaccctt atactcttaa tattaaactt atgcaagaag
      781 ctattaacgg tctggtaggt acacacgatt ttactggttt tacagcagca ggaacttcgg
      841 tacaaaataa ggtgcgaacg attaccaaag caacggtatc tcgggatgaa aaaactgatt
      901 ttttggtgtt tactttttca ggaaatggtt ttctctataa gcaggtgagg aatatggtgg
      961 gaactttgct gaaaattggt aatggtcaaa tgccagttga acaagttaaa gtgattttga
     1021 gctccaaaaa tcgtcagcta gctggaccaa cgatatctgg aaatgggctt tacttgaagg
     1081 agatttgtta tgaaaactga ttatatcgtg actatatctg ggaacgatat tttaagtgga
     1141 ggtggtctct atgctgattt agcgacttac ataaggtatg acctacaggc ctttgtagcg
     1201 gtaacttgtt taactactag atctgaagag ggctttagtc tatttccggt agctaaagaa
     1261 attttccgtg atcagttgaa tagctttacg aacgctccaa tttctgctat taaaattggt
     1321 ttgttaccta atgcggaaat gtgtgaaata gttttggatt ttataaaggg gcatctggga
     1381 atcccagtgg ttttagatcc tgttctagca tgtaaagaaa tagatgatgt taaaatagtt
     1441 cctctaagat aggagatttt acaattattg ccttatgtaa ctgttgttac tccaaatttg
     1501 gtagaggctc agctattatc tcaaaaagag attgtttctt taaaagatat gcaagaagct
     1561 gctaaatatt tttatcaact tggggcaaaa caggtggtta tcaagggtgg taatcgattt
     1621 agtcagaaaa aggctattga tcttttttat gacggtaaag agattgttac cttggaatgt
     1681 cctgtacttg agaaaaataa tattggcgca ggctgtactt ttgcatctag tatcgctagt
     1741 cagttagtaa agaaaaaaac gccactagaa gctgtgaaaa actcaaaaga gttagtctac
     1801 caagctattt tacaatctga tagatacgga gtaaaacaaa gttatgcaaa atagtaagat
     1861 acgacaaatg agtttaacag ggatattgac agcattggtt gttgttttag gaagatttgt
     1921 catgctacct actccaacag gttttttaac gctgttggat gcaggaattt atgctgtcag
     1981 cttttccttt ggatcagctc aaggagctat tgtgggaggg ttatcaggtt ttttaatcga
     2041 tttagtggca ggataccctc agtggatgtt tcatagtttg attgctcaca gtgtccaagg
     2101 gtattttgcg ggatggagag ggcgcaagcg ttggcttggt gttgtgatag gttcttttat
     2161 catgattttt tggtactttt taggctctct tatgcttggt tatggccttt cgggatcttt
     2221 agccggtatt tggggaaacg tcatgcaaaa tacattaggg ctttttgttg gttttattat
     2281 ctttaaggcc atattaaggc aaaaaaagag ataaggagag tgagaagttg ttgaataatt
     2341 tagagaaaca aacaagggaa atcgtcattg atgttgtaga acgctctgct attcaaccag
     2401 gtaatctttt tgttctaggt ctttcatcta gcgaaatttt gggtagtaga attggaaaac
     2461 aatctagtct agaagtaggt caaatagttg ttgaagttgt gttagatgaa ttaaataagc
     2521 gtggtgttca tttggctgta caaggttgtg aacacgtcaa tcgtgccttg gtagtagaga
     2581 ggcatgtggc agaatctaag cagttagaga ttgtcaatgt ggttccgaat cttcatgctg
     2641 gtggcagtgc tcagatggct gcttttcaat ttatgtcaga ccctgtcgaa gtagaagaag
     2701 ttattgcaca cgctggactt gatataggtg atactgctat aggaatgcat attaagcgtg
     2761 ttcagatccc tctgattcct tgtcaacgag agctaggagg tgctcatgtg acagctttgg
     2821 ctagtcgacc aaaattaatt ggtggagcta gggctgatta caatatggat attatccgta
     2881 aatcctaaac ccttttttct ggaataaaac aattggtgtt ttttctagaa aaaaggattt
     2941 tttaagatta tattaagatt ttgaggtgat agaatagctg attgcagtcg gtaattgaat
     3001 gccttctttg agtaaagctt cttgataaag tctataaaaa gtatggtaaa tcttaaattg
     3061 ttctccattt tctgtgaaaa tagcaattct aaaagtgaac tgtccattag agttattttg
     3121 aggccctaaa atatttggtt tgccaacgat ttgaggatgg tttggcaatt ccttttgatt
     3181 cacttcatca atgattcgag tgacttggct caaattaaca gtggaataaa gaggaatttc
     3241 aattaaggca cgcatattac cacgagattt gttgctgaca acggtgatgc tacggtttgg
     3301 gatgaaatgt agtgttccat cgaatccacg aatttgagta gttctaattc caacaccaaa
     3361 gacactgcct tctatgtcag agatagtaac gttatctcct acttcaaatt gattctcaaa
     3421 taagataaaa aagccattga caacgtcaga taaaaatccc tgtgcaccta acccgattgc
     3481 tactccagca attccagctc ctgctagaag actagagaca ggtatgccaa ataagctaag
     3541 tatccaataa atgagaagaa aatacaataa gtaatttaaa atattgtgag tgagcttaga
     3601 gagtgttttt ttgcgagctt cactttgtct tgaataagca aacgatttgt taatggtttt
     3661 ttcaaaaaga tagttagtaa cacgttttaa aatagcaaaa aagagtaata gaataatgag
     3721 agacactagc tttttaaaaa tcgttaagcc aatattttcg atatgagact gctctaggta
     3781 atgtaatata aatgtcatga gaaatccttt ctttgatagt ttatcaaaaa aaaaccaatc
     3841 ttgaaaagag ataaattaat attttaacca aagttatgat aattgtttta tcataaagaa
     3901 tgccatagtc atccgttgtg ataatgcttg aaaatagtca gttagtaagc gaaaaagtct
     3961 aacattaacc tttataaatt gaaaataact gttagtagac taatgacagg tagatactct
     4021 taatagcgcg tttgatttca cgcataaaaa gctttaaaaa cgctttaaat tatgataaaa
     4081 tgaactgtat cgaaattatt ttaaggagaa atgacttaat gtctacatca tttgaaaaca
     4141 aagctacaaa tcgtggcgtg attacattta caattagtca agataaaata aaaccagctc
     4201 ttgataaagc ttttaataaa atcaaaaaag acttaaatgc accaggtttc cgtaaaggac
     4261 acatgcctcg tccagtcttc aaccaaaaat ttggtgaaga agttctttat gaagatgctt
     4321 tgaatattgt attgccagaa gcttatgaag cagctgtgac ggaacttggt cttgatgtgg
     4381 ttgcacaacc aaaaatcgat gttgtgtcaa tggaaaaagg gaaagagtgg acactttctg
     4441 ctgaagttgt gacaaaacct gaagtgaaac ttggtgatta caaaaacctt gttgtagaag
     4501 ttgatgcttc aaaagaagtt tcagatgaag acgtggatgc taaaattgag cgcgaacgtc
     4561 aaaaccttgc ggaactcatt attaaagacg gtgaagcagc tcaaggtgac actgttgtga
     4621 ttgactttgt tggttcagtt gatggtgttg agtttgatgg cggtaaagga gataacttct
     4681 ctcttggact tggttcagga caatttatcc caggttttga agatcaacta gttggtgcta
     4741 aagctggcga tgaagtagaa gttaatgtca cattcccaga atcttaccaa gcagaagatc
     4801 ttgcaggtaa agccgctaaa tttatgacaa ctattcacga agtcaaaaca aaagaagtac
     4861 cagagcttga tgatgagctt gcaaaagata ttgatgaaga tgttgacaca cttgaagact
     4921 taaaagtaaa atatcgtaaa gaacttgaag cagctcaaga aactgcttat gatgatgctg
     4981 ttgaaggagc tgcgattgaa ttagcagttg caaatgctga aattgttgat ttacccgaag
     5041 aaatgattca tgaagaagtc aaccgttcag tgaatgaatt tatgggcaac atgcaacgtc
     5101 aaggaatctc acctgaaatg tacttccaat tgactggtac aactcaagaa gatttacata
     5161 accaatattc agctgaagct gacaaacgtg ttaaaacaaa ccttgttatt gaagcaattg
     5221 ctaaagcaga aggttttgaa gcaacagata gtgaaattga acaagaaatt aatgaccttg
     5281 caacagaata taacatgcca gctgaccaag ttcgttctct tctttcagca gatatgttga
     5341 aacatgatat tgcaatgaaa aaagcagttg aagtgattac aagcacagca agcgttaagt
     5401 aatcttatta aggaacaaaa cgtacaagcc ggattagaac gtctttctag tctggttttt
     5461 ctttttctaa agataaggaa ttagttaaac gttattttaa tgaattagct ttaaagattt
     5521 gtcaagaaga agtgatatga gttgataaaa gttttgacga attacttttt ttagcgtaaa
     5581 atggtagaga atgataaaat aaggagaact gccttgaaat tagacgtatt tgcaggacaa
     5641 gaaaaaagcg agctttccat gattgaagtg gcgcgtgcca ttttggaaga acgcggtcgc
     5701 gacaatgaga tgtactttag cgatttagtc aatgagattc aaaattactt aggaaaatct
     5761 gatgcaggta ttcgtcatgc attaccattt ttctatactg acttaaatac ggatggctca
     5821 tttattcctc taggtgaaaa caaatgggga cttcgttcgt ggtatgctat tgatgaaatt
     5881 gatgaagaaa ttattactct agaagaagat gaagatggcg cacaaaaacg taagaaaaaa
     5941 cgcgttaatg ctttcatgga tggagatgaa gacgctattg attatagaga tgatgatcca
     6001 gaagatgaag atttcacaga agaatcagct gaggttgaat atgatgaaga agatccagat
     6061 gatgaaaaat cagaagtgga gtcatatgat tcagaattga atgaaattat ccctgaagat
     6121 gattttgaag aagttgatat caatgaagaa gacgaagaag atgaagaaga tgaagaacca
     6181 gttctctaat gtctttagtt cagaaataaa aatgtgatta tcatttgaca aattgtctat
     6241 tatagtttat attattattc gggcacctct ttatgaggtc aggcaaaagc tctctagtta
     6301 atagggggct atttttgttt tctagaataa tgccagtttg cctctatatt tagaaaacta
     6361 aggtgatacc taaccaatgt ggaaccaact aatagggaat taacaagaaa gcaatgcttt
     6421 tattcagctt atataaagga gtactatgac gaaatacatt tttgtaactg gtggtgttgt
     6481 atcatcaatt ggcaaaggaa ttgttgcggc aagtcttggc cgattgttaa aaaatcgtgg
     6541 acttaaggtg acgattcaaa aatttgatcc ttatattaat attgatccag ggacgatgag
     6601 cccctatcag catggagaag tatatgtgac agatgacggt gctgaaaccg acttggattt
     6661 gggtcactat gaacgtttta ttgatatcaa tcttaataaa tattccaatg ttacaacagg
     6721 taaaatttat agtgaagtcc ttcgtaaaga acgtaaaggt gagtaccttg gagcgactgt
     6781 tcaggtgatt cctcatatca cagatgccct taaagaaaaa atcaaacgcg cagcttcaac
     6841 aacggactct gatgtaatta ttactgaggt cggggggact gttggcgata ttgaaagttt
     6901 accgttctta gaagctcttc gtcagatgaa agctgatgtt ggttctgaaa atgtgatgta
     6961 tattcacacc accttgttgc catatttaaa ggcagcgggt gaaatgaaaa ctaagccaac
     7021 gcaacattcc gttaaagaat tgcgtggttt agggattcaa ccgaatatgt tggttattcg
     7081 tacggaagag ccagttgagc aggggattaa aaataaattg gctcagttct gtgatgtgaa
     7141 ttcagaagct gttatcgagt cacgtgatgt agaacacttg tatcaaattc cattgaactt
     7201 gcaagcccaa tcaatggatc agattgtttg tgatcatttg aaattgaatg cgccacaagc
     7261 tgatatgact gagtggtcag ctatggttga caaggtgatg aatttaagaa aaacaacaaa
     7321 aattgccttg gttggtaaat atgtggaatt gccagatgcc tacttatctg ttgtggaagc
     7381 cttgaaacat tctggttatg ccaatgatac cgcaattgac ctaaaatggg tcaatgctaa
     7441 cgatgtgacg gtggataatg cagctgactt gttgggagat gcggatggga ttattgtacc
     7501 aggtggtttt ggtcaacgtg gtacagaagg caagatccaa gcgattcgct atgcccgcga
     7561 gaatgatgtg ccaatgcttg gtatctgtct tggcatgcaa ctgacttgtg ttgaatttgc
     7621 tcgccatgtc ctcaatatgg aaggtgcaaa ctcttttgaa ttagagccaa gtactaaata
     7681 ccccatcatt gatatcatgc gtgatcaaat tgatattgaa gatatgggag gtacgcttcg
     7741 tctgggattg tacccatgta aattgaaacc aggttcaaaa gcagcaatgg cttacaataa
     7801 tcaagaggtg gtgcaacgtc gtcatcgtca ccgttatgag ttcaacaaca aattccgtcc
     7861 agaatttgaa gcagctggtt ttgttttctc aggggtatca cctgacaacc gcttagttga
     7921 aattgtagaa ttgaaagaga aaaaattctt tgtagcagct caataccacc cagaattgca
     7981 aagccgtcca aatcgtcctg aggagctata caccgctttt gttacagctg ctattaaaaa
     8041 tagcaattag tggttttaag ggtaagtttg tggcattcta cttggctggc agatactgat
     8101 aaagattact agctattaga gggatctttt ctataaaaat agcattagaa aaagaaaaac
     8161 gtgatatttt taggttagca ctttcttttt cagtcatttt tttgtaaaat acttggtatg
     8221 aaaactattc gaatagcaaa gtatttagga attctattcc ttttgataac tcttattagc
     8281 gtaggtgcta gtttttattt ctttcatgtt gcacaaataa gagaagagaa atcgtttatt
     8341 aataataaga aacggagtac aaataatcca ttatacccag ctgaacagtc ttttgacgct
     8401 ttaccttacg aaaaacgtca actaacaaat cgtgggttaa aacaagtggg gtggtactta
     8461 ccagctgctc aaaaaacaaa aaagacagct attgttgttc atggttttac gaatgacaaa
     8521 gaagatatga agccatatgc catgcttttt catgatttgg gctataatgt cttaatgcca
     8581 gacaatgagg cccatgggga aagtgaaggg aacttgattg gttatggctg gaatgaccgc
     8641 cttaatgtca tggcttggac agaccaactg attaaggaaa accctgaaag ccaaatcaca
     8701 ctctttggct tatctatggg tgctgcaaca gtaatgatgg caagtggtga gcgattgcct
     8761 gcgcaagtca cctccctcat cgaagattgc ggttatacca gtgtttggga cgaattgaag
     8821 tttcaggcca aggctatgta caacttgcct gcctttcctt tactctatga agtctctgcc
     8881 ttatctaaga ttcgagcagg ttttagttac ggagaagcga gctcagtgaa acagctggct
     8941 aaaaataaac gtccaacttt atttatccac ggtgataagg atgattttgt tcctacaaaa
     9001 atggtttatg acaattataa ggccacgaaa ggtcctaagg aaatcttgat tgttaaaggg
     9061 gcaaaacacg ccaaatcctt tgaaacaaac ccagaacaat accagaaaaa aattgccgct
     9121 tttttgaaaa aagttgagaa ataagtattg acagttaaac tcacagttgc tataataatt
     9181 tatgttactg atgagtagcg gggatggcgg aattggcaga cgcgcaggac taaggatcct
     9241 gtgaccgctt taggtcgtgt gggttcaagt cccactctcc gcataatgta agaggtgaat
     9301 cattgattcg tctctttttt ttggttaaat gaaggacaag aaggcataga tttactggag
     9361 aaaacttttg ataatctgct tttggaaaac gctttaagct tgtgaaagac tagaaattta
     9421 tggaaaattg tgctagaatg aacaatgtaa acggggagtt atcccgaaaa ataagaggag
     9481 gccttacaaa tggcaatcgt ttcagcagaa aaatttgtcc aagcagctcg tgaaaacgga
     9541 tatgctgttg gtggatttaa cacaaacaac ttagagtgga ctcaagcaat cttgcgtgca
     9601 gcagaagcta aacaagctcc agttcttatc caaacatcaa tgggtgcagc aaaatacatg
     9661 ggtggttata aagtatgtca aactcttatt actaaccttg ttgagtcaat gggtatcact
     9721 gttcctgttg ctatccacct tgatcatggt cactacgaag atgctcttga gtgtatcgaa
     9781 gtaggttaca cttcaatcat gtttgacggt tcacaccttc cagttgaaga aaaccttgct
     9841 aaaactgcag aagttgttaa aattgcacat gctaaaggcg tttcagttga agctgaagtt
     9901 ggtacaatcg gtggtgaaga agacggtatc attggtaaag gtgagctcgc tccaatcgaa
     9961 gatgctaaag caatggttga aactggaatt gacttcttgg cagctggtat cggtaacatc
    10021 cacggtccat acccagaaaa ctgggaaggt cttgctcttg accacttaga aaaattaaca
    10081 gcagctgtac caggtttccc aatcgtattg cacggtggtt ctggtattcc tgacgatcaa
    10141 atcaaagaag caatccgtct tggtgttgct aaagttaacg ttaacactga aagtcaaatc
    10201 gctttctcta acgcaactcg tgaatttgct cgtaactacg aagctaacga agcagaatat
    10261 gatggtaaaa aattatttga cccacgtaaa ttcttggctc caggtatgaa agctgttcaa
    10321 ggtgctgttg aagaacgtat cgatgttttc ggatcagcta acaaagcata atttagagta
    10381 ttaaatactc taaagccctc ttgagggctt ttttagatac aattgacacc ctaaaatgga
    10441 taccagtttc tgtgaaacaa tctttgattg tgaacaaaca aagcataatt tagagtatta
    10501 aaatctggac cctgctatct atggtaagtc tttttgttat tgttaaagag gttaagcgga
    10561 atgttaatat gttttagcta aaaaatgtag tgtaaaatga gtacgtagac tattgtaata
    10621 gtaattccgt aaaattgttg aaaaagaaaa tgggaaatac cttgtcaaat taagcaccct
    10681 atgatagaat agataggtat gtggtgctag cacatccgta aacatattct acgtaggagg
    10741 aaacagatca aaatggctaa agtatgttat tttacaggac gtaaaaccgt ttctggaaac
    10801 aaccgttcac acgcgatgaa ccaaacaaaa cgtacagtta aaccaaacct tcaaaaagtt
    10861 actatccttg ttgatggtaa acctaaaaaa gtttgggctt ctgctcgtgc gcttaaatct
    10921 ggtaaagtag aacgcatcta atagaaataa gccttagggc tttttttatt agcttatttc
    10981 tatccataag ctagtcaggg taataaacgc tatcgttaac tattctatct cttttacatt
    11041 acttctaaaa tatgataaaa taggatgata aaaacttttt gaggtagtaa ctatgactgt
    11101 aaaaattaat acaaaagatg gtctaatcga gctatccgac gatgtgattg caactgtcgt
    11161 gggaggatca gcaacggaaa tttttggtgt tgttggaatg gccagcaaaa gtgcaataaa
    11221 agacaatttt caatcactac ttcgcaaaga aaactacgct aagggtgttg tagtaaaatc
    11281 aacagatctg ggtatttctg ttgatgtcta cactgtgatg agttatggtg tgaaaatcag
    11341 tgaagtctct aaaaatatcc aagaacgcgt gaaatttaac cttgaaagtc agcttggtct
    11401 cactgctgat atggtcaatg tttacgtgca aaatattaag gttgtaggag aaaattagtg
    11461 tcaaatatta caacaagctt attccaagaa atggtccaag cggcagcgac tcgccttggc
    11521 aaacaagcag aatatgttaa ttccttaaat gtttttcctg ttccagatgg tgatacagga
    11581 acaaacatga gtatgactat ggacaatggt gccaaggaag ttgctgataa acctgcgtca
    11641 actgtgggag aagttggaca aatgttatca aaaggtcttt tgatgggagc gcgtggaaat
    11701 tctggagtta ttacttcaca actttttcgt ggttttggtc aaagtattaa gggaaaagat
    11761 gagctgacag gtaaagattt ggcacaagcc tttcaagtgg gtgtagaagt tgcctacaaa
    11821 gcagtgatga aacctgttga agggacaatc ttgacagtat ctcgtggagc agcaaccgct
    11881 gccttaaaaa aagcagatct tacagacgac gccgtggaag tcatgcaagc tgctttagac
    11941 ggtgccaaag gtgcccttgc taagacacct gatttattac cagtccttaa agaagttggt
    12001 gtggttgatt caggcggtca aggtcttgtc tttatctatg aaggtttcct atctgctttg
    12061 aatggcgact atgtgacttc agcagacttt aaagcgactc ctgctaacat gtctgaaatg
    12121 attaatgctg agcatcacaa atccgttgtt ggtcatgtgg ctactgaaga cattacctac
    12181 ggttattgta ctgaaatcat ggttgccctt aaacaaggcc caacctatgt gaaagaattc
    12241 aactatgatg agtttcaagg ctaccttagt ggccttggtg actcgctgtt agtggtcaat
    12301 gatgatgaga ttgttaaagt tcacgtccat actgaagacc ccggacttgt gatgcaagaa
    12361 ggtcttaaat atggttcgct catcaagatt aaagtggaca atatgcgtaa ccagcatgaa
    12421 gcacaggttc aaaagactga tgtcgaaaaa accaaggctg aagtaaaaga ctttggcttg
    12481 attgcagtag tagctggaga aggtttatct gaaattttca aggcgcaagg tgttgattac
    12541 gtgatttctg gtggtcaaac catgaatcca tctacagaag atattgttaa agctattgaa
    12601 gcagttaatg ccaaacaagt cattatcttg ccaaacaata aaaatatctt tatggctgcc
    12661 caatctgctg cagaagtggt ggatatcccg gctgctgttg ttgcaacacg tactgttccg
    12721 caaggattta ccagcttgtt ggcctttgat ccttcaaaat ctttagagga caatgtagca
    12781 gatatgtcaa ctagtctttc tgacgtggtc agtggaagcg ttactttggc agtccgtgac
    12841 accaccattg atggtttaga aatccatgaa aatgatttct tgggaatggt tgatggtaaa
    12901 atcatcgttt caaacccaga catggaagca actctaaaag ctgcctttga aaagatgatt
    12961 gacgaagata gcgagattgt gactatcttt gttggtgaag aaggtgatca agacttagca
    13021 gaagaacttg ctgggtattt aggagagact tacgaagatg tggaagttga gatccatcaa
    13081 ggagatcagc ctgtttaccc atatctcatg agtgtagaat aatagaatac aataaaaaaa
    13141 gaggagttag gcaaaggtct gactcttttt gtctatattg atgtcgtaat gagtggtttt
    13201 agtgatggta agcccctaaa aaatgagggt aatccatcac aaagctaatg gttacgattt
    13261 aatttgacaa aacctagaaa aacattataa ttgtattata acaataagtc aagaactgaa
    13321 aacggttcag ttctaagata tgacaaggag gagattttat gttaggacca tttatcttta
    13381 ttgcttttgg ggttattgta atcctagcta ttgttgccag taccctttat gttgttcgtc
    13441 aacaatctgt tgccatcgtg gaacgttttg gtaggtacca aaaaacagcc acgagtggta
    13501 ttcatgtccg ccttccgttt gggattgaca aaattgctgc gcgtgtgcag ctgcgtcttt
    13561 tacagtcaga aatcatcgtt gaaaccaaaa ccaaggacaa tgtgtttgta actttgaatg
    13621 ttgccactca gtatcgtgtt aacgaacaaa atgtaacaga tgcttactac aaattaatga
    13681 aacctgaatc tcaaatcaag tcttatattg aagatgcctt gcggtcatcc gtgccgaagt
    13741 tgaccttaga cgaattgttt gagaaaaaag atgaaattgc cttggaagta caacatcaag
    13801 tggcagaaga aatgtcaacc tatggatata ttattgtcaa aaccttgatt accaaggtgg
    13861 agcctgatgc tgaagtcaaa cagtcaatga atgaaatcaa tgcggctcaa cgcaaacgtg
    13921 tagctgctca agagttggca aacgcagata aaattaaaat tgtgacagca gcagaagcag
    13981 aggctgaaaa agatcgattg cacggggtag ggattgccca acagcgtaag gccattgtgg
    14041 atggtttggc ggagtctatt caagagctta aagaagcgaa tatttcctta aacgaagaac
    14101 aaatcatgtc cattttgttg accaaccaat acctggatac cctcaatacc tttgcggcaa
    14161 aaggaaacca aacccttttt ctaccaaata caccaagtgg ggtagaggat atccgtacac
    14221 aagttttatc tgcccttaaa acaaaataac gtgtaaaagc ttggctaaaa agttgctcaa
    14281 tactggattt agcagaaggc tatcatacct tgtgaagtgg caaacgacga aatgaataca
    14341 ttacttttat tggagaaatg aaagtaaaaa agcctttttc atgattaaca acatagtatt
    14401 gattaaatgt taagttgtat tgacttttaa attaaataag tttaaaatca acttaataag
    14461 caaaaaggag attttcaatg aaatccaaaa aagttgttag tgttatatca cttaccttat
    14521 cccttttttt ggtgataggt tgtgctaaag ttgataacaa caaatcagtt aatcgtaagc
    14581 ctgctactaa acaaacctat aatagctata gtgatgacca attaagatcg cgtgaaaata
    14641 ccatgtctgt tttatggtac cagcgagctg cggaaactca agcgctttat ttacaggggt
    14701 atcagttagc aacggatcgc ttaaaagaac aactcaataa accaacggat aaaccttatt
    14761 caattgtatt agatattgac gaaacagttc ttgataatag cccttatcaa gctaaaaatg
    14821 ttttggaagg aacaggattt acacctgaaa gctgggatta ttgggtacaa aagaaagaag
    14881 caaaaccggt tgctggtgct aaagactttt tgcaatttgc agaccaaaat ggtgttcaaa
    14941 tttactacat ttcagacaga tcaactactc aagtagatgc tacaatggaa aatctccaaa
    15001 aagaaggtat tccagtacaa ggtcgtgatc atcttctatt cttagaaaaa ggcgtaaaat
    15061 caaaggagag tcgtcgtcaa aaggtcaaag aaacaactaa tgtaacgatg ctatttggtg
    15121 ataatcttct agattttgct gatttttcaa aaaaatctca agaagataga acagctttat
    15181 tatcagattt gcaagaagag tttggaagac gctttatcat tttccctaat cctatgtatg
    15241 gttcatggga aggtgccatt tataaaggtg aaaagctgga tgtgcttaag caactagagg
    15301 aacgccgtaa aagtttaaaa agctttaaat aaagtaattg aaaaagaggt gcaaaagaaa
    15361 atgtaacctc tttttaagta ctattatttt ctgaaaatag ggttaatgac tgaaaaaaat
    15421 gttgattttg tgaaatgatt attttattat tttcatattt tatctttatg ctaggaattg
    15481 tggtataatg gaggagtata aaaaatatta aggagtctat tcacatggct aaattgactg
    15541 ttaaagacgt tgatttgaaa ggtaaaaaag tcctcgttcg tgttgacttt aatgtgcctt
    15601 tgaaagatgg cgttatcact aacgacaacc gtatcactgc ggctcttcca acaatcaagt
    15661 atatcatcga acaaggtggt cgtgctatcc tcttctctca ccttggacgt gttaaagaag
    15721 aagctgacaa agaaggaaaa tcacttgcac cggtagctgc tgatttagct gctaaacttg
    15781 gtcaagatgt tgtattccca ggtgttactc gtggttcaaa attagaagaa gcaatcaatg
    15841 ctttggaaga tggacaagtt cttttggttg aaaacactcg ttttgaagat gttgacggta
    15901 agaaagaatc taagaatgac gaagaacttg gtaaatactg ggcttcactt ggagatggaa
    15961 tcttcgttaa cgatgcattt ggtacagcac accgtgctca cgcatcaaac gtaggtattt
    16021 cagcaaacgt tgaaaaagct gtagctggtt tccttcttga aaacgaaatt gcttacatcc
    16081 aagaagcagt tgaaactcca gaacgtccat tcgtagctat tcttggtggc tcaaaagttt
    16141 ctgataagat tggtgttatc gaaaatcttc ttgaaaaagc tgataaagtt cttatcggtg
    16201 gtggtatgac ttacacattc tacaaagctc aaggtatcga aatcggtaac tcacttgtag
    16261 aagaagacaa attggatgtt gctaaagacc tccttgaaaa atcaaatggt aaattgatct
    16321 tgccagttga ctcgaaagaa gcaaacgcat ttgctggtta cactgaagtt cgcgacactg
    16381 aaggtgaagc agtttcagaa ggcttccttg gtcttgacat cggtcctaaa tcaatcgctg
    16441 agtttgacca agcacttact ggtgctaaaa cagttgtttg gaacggacct atgggtgtct
    16501 ttgaaaaccc tgacttccaa gctggtacaa tcggtgtcat ggacgctatc gttaaacaac
    16561 caggcgttaa atcaatcatc ggtggtggtg attcagcagc agctgctatc aaccttggcc
    16621 gtgctgacaa attctcatgg atcagtaccg gcggaggcgc tagcatggaa cttctcgagg
    16681 gtaaagtatt accaggtttg gcagcattga ctgaaaaata atagtcatta taatatatag
    16741 taaagggcat gctgatgcat gtcttttttt agttgatcat gtacagttat tgaactttcc
    16801 ctaacttcac ttatcattta ctagttttta gatatagtgg tatttggctg acactgcaat
    16861 tttcaaaagt ttatgacgat ttcatatcaa ttggtttata aaagatatcc taatcataaa
    16921 taaaacgttt accaaaaggg ttttatggat tgatatgtta aaatagatag tgaagggata
    16981 ggtatgaaaa cgttaagaaa attattatct aattacaagt ttgatattaa aaagtttaaa
    17041 ttgggaatga gaacattgaa aacaggctta tcggtctttt tagttttatt ggtttttcat
    17101 ttatttggtt ggaaagggct tcaaattggt gctctaactg ctgtatttag tttaagagaa
    17161 gactttgata aaagcgtgca ctttggcttt tctagaatta ttggtaatag tataggtggc
    17221 ctattctcct tagtattttt tgcattcaat gagatatttc atcaagcttt ttgggttact
    17281 ttattaattg ttcctatttg taccatgtta tgtatcatgg ttaatgttgc atgtaataat
    17341 aaatcaggta ttattggggc agtggctgct cttttgatta ttacactgtc tattccaaca
    17401 ggacaaacgt ttatttatgt gacttcgcgg gtatttgaaa cattttgtgg cgtctttgta
    17461 gctattttag ttaatactga tgttgaactt attaaaaata aatggtttaa caaaaaacac
    17521 agtgatgtta gataatataa catatatact tgacaggtta tttttttcag cctataataa
    17581 ggaagatgaa aggagtttgt catgaaagaa aaagaactta gacgctcaat ggctgttttt
    17641 cctattggaa cggtgatgac attaacagat ctctctgcga gacaaattcg ctattatgaa
    17701 gatcaaggtt taattaagcc tgaacgaacg caagggaatc ggcgtatgtt ttccttaaat
    17761 gacatggacc gacttttgga aattaaagat tttttgtctg agggattaaa tattgcagct
    17821 attaaacgcg aatatgtcga gcgtcaaggt aagttaatgc aaaaacaaaa agctctaaca
    17881 gatgcagatg tcagacggat cttacatgat gaaatgctga cacaaagcgg tttttctact
    17941 ccctcacagc atattggtaa ttttcgtatt taagatatga attgtataaa atttaaagga
    18001 gacctattta atggcaataa cagtagctga cattcgtcgt gaagtcaaag aaaaaaatgt
    18061 aacgtttctt cgcttgatgt tcactgatat catgggcgtt atgaaaaatg tggagattcc
    18121 tgcaactaaa gaacagttag acaaagtatt gtctaacaag gttatgtttg atggttcatc
    18181 tatcgaaggt tttgtacgga tcaatgagtc agatatgtac ctttaccctg atttagacac
    18241 ttggattgtt tttccctggg gagatgaaaa tggagcagtt gcaggtttaa tttgtgatat
    18301 ttatacagca gaaggaaagc cttttgcagg agatcctaga ggaaatttaa aaagagccct
    18361 gaaacacatg aacgagatcg gctacaaatc atttaatctt ggaccagaac cagaattttt
    18421 cctttttaag atggatgata aaggtaatcc gacacttgaa gttaacgata atggtggtta
    18481 ttttgattta gcgccaattg acttagcaga caacacgcgc cgtgaaattg tgaatatttt
    18541 aacgaaaatg ggttttgaag tggaagctag tcatcatgaa gtggctgttg gtcaacatga
    18601 gattgatttt aaatatgcag atgttttgaa agcttgtgat aatattcaaa tttttaagct
    18661 agttgtaaaa acgattgccc gtgaacatgg actttatgct actttcatgg ctaaaccaaa
    18721 atttggaata gctggatcag ggatgcactg taacatgtct ttgtttgata accaaggtaa
    18781 taatgctttt tatgatgaaa ctgataagcg agggatgcag ctatcagaag atgcttatta
    18841 tttcttggga ggactaatga agcatgctta taactacact gctatcacta accctacagt
    18901 gaattcttac aaacgattag ttccgggtta tgaggcacct gtttatgtcg cttgggctgg
    18961 aagtaatcgt tcaccgctta tccgtgttcc agcatcacgt ggtatgggaa cgcgtttgga
    19021 gttacgttcg gttgatccga cagctaatcc ttatttagcc ttggctgttc tcttggaagc
    19081 tggattagat ggtatcatta acaaaattga agctccagaa cccgttgaag ctaatattta
    19141 taccatgaca atggaagaac gaaatgaagc aggcattatt gatttgccat caacgcttca
    19201 taatgcctta aaagctcttc aaaaagatga tgtggtacaa aaggcactag gataccatat
    19261 ctacactaat ttcttagaag caaaacgaat tgaatggtct tcctatgcaa cttttgtttc
    19321 tcaatgggaa attgaccatt atattcataa ctattagagt tatcaggacc tgctcaagtt
    19381 gagtgggtct tttggatgag ctattgtatt tgagctagca agctattatt agctgatcgg
    19441 acaccaattg attttccatt atagaaaact aaaaaccagt ccatgttcta tgaggactgg
    19501 tttttagttt gataattatc tgaactagaa ctgatatgtt aaagatatag acagatagta
    19561 ttaagaaatg agtcttattt gattttagtg cttatcaggt gttaagacca tcggaattat
    19621 aattggttca cgctcagtct tttcatacaa gaatggacga agagcattga cgatagcacc
    19681 gttaactgat tggatgctag catccttatt ttttaaagca atacggatag cgttaaagag
    19741 cacacgttgg ctttcgcgga tcaagtcacc tgactcacgc atataaatga aaccacggct
    19801 cagaatatct ggtccagcta aaatcatttg tgtgttaaaa tcaacagttg caacagctaa
    19861 gacaacacca tcttctgaca aatcacggcg gtctcgtaaa acggctgctc caatatcccc
    19921 gatgccatta ccatccacgt agatgtcttg ggcattaaag tgaccagcaa tacgcgcact
    19981 gtcactggtt aaagctagaa cgtctccatt ttccataata aagatatttt cttttggaat
    20041 accaatatcc atcgccagtc ctgcatgaac tttttgcatg cgatactcac catgtactgg
    20101 catgaaatat tttggtttaa tcaaactaag catgagcttt tgttcttgct gtcctccatg
    20161 accagaagta tgaatattat tgacttttcc atgaataaca tcaacaccag cttcttggat
    20221 ggtattgatt aatttattga cacttgtggt attaccaggg attggacttg atgagaaaat
    20281 cacagtatca ccaggctgta aagttacttg acgatgagtg ccattagcga tacgagcaag
    20341 tgctgccata gactcacctt gactacctgt acacataatt aaagcttcac tagcatgaag
    20401 atttttaagt tcgcttggct caatgaaggt accttttgga actttaatat agccaagttc
    20461 aataccgtta acaattgctt tttccataga acgaccaaaa acggcaattt tacgtcccgt
    20521 tttaacagca gcttctgcag cctgttgcaa acggtagatg tttgaagcga aggaagcaaa
    20581 aataatacgc ccatgaatac cttcgataat ttttaaaatg gattggccaa caactttttc
    20641 agagttagta aaggttggga tttcagcatt agttgagtct gataataagc aaaggacacc
    20701 ttcttcacca agcgctgcca tacgttgtaa atctgcagga tctcctactg gcgtgaagtc
    20761 aaatttaaaa tcaccagtac agataatctt accttgaggg gtgtgaataa caataccgac
    20821 cggttctgga atggaatgag tagtcttgaa gaaagtgacg ctcatgtttt taaaagttaa
    20881 ctcggtgttg tgattgattt cgtaaacagt tgcttcacgc caaagaccat gttcttccaa
    20941 tttaccacgg ataagagcaa gtgctaaagg tcctgcgtag atagggatat tagcctgttt
    21001 gagaagaaat ggaataccac caatgtggtc ttcgtgacca tgtgtaataa caagagcttt
    21061 gacccggtct aagttatcga caatgtaaga atagtctgga ataacgtaat caattcccaa
    21121 aagatcatct tctgggaatt tgataccggc atcaacgata atgatctcgt cttgatactc
    21181 aataccataa gtattcttcc caatctctcc cagaccacca atggcaaaca ccccaacttc
    21241 attaggtttt aaactgatat ttgtcatctt agaactccgt taattcaaaa acaccggtct
    21301 ctttttcgta atcgaggtgt ttgtcagaaa gtagctcgat aaattccacg ttataatagg
    21361 tgttatcttc aacgagacga cgagccttga tacggccatc gagttcatca gtagcatcaa
    21421 tattaagata gagtgcttta gtactttcac gacgtgggct ttgatccttt gtttcttgat
    21481 agaaaacttt ataaatcatt tgtttttttt cctttcgtta tgaatcaatt atccaaacaa
    21541 taataaatag gtcaaaaggt aggtgttcca ttttgacggt tttatatgtt gtcattattg
    21601 tgtagtagat taattcctga ttctgtgcaa ttattacaat tataccataa acttagcttt
    21661 tagtaaaaaa tttaggtaca tttatggtat agtctaaatc aaattaagag tcagcctttt
    21721 tgtttatgat tcttaatttg tgttataatt gatactagaa cttgcagtag aaaggcataa
    21781 aatgaagaca cttgcatttg atacctcaaa taaaaccttg tcccttgcta tacttgatga
    21841 tgagacactt ctagcagata tgacccttaa cattcagaaa aatcatagtg ttagccttat
    21901 gcctgctatt gattttttga tgacttgtac tgatcttaaa cctcaagatt tagaaagaat
    21961 agtggttgca aaaggccctg gatcttacac aggtttacga gtggcagttg ctactgcaaa
    22021 aacgttagcg tacagtttaa atattgcatt ggtcgggatt tcgagtctat atgctttggc
    22081 tgcgtctact tgtaaacagt atccaaatac tttggtggtg ccattgattg atgctagaag
    22141 gcaaaatgcg tatgtaggtt attatcggca aggaaaatca gtgatgccac aagcccatgc
    22201 tccactagaa gttattatag aacaattagt agaagaaggg cagctgattt ttgttgggga
    22261 gactgctcct tttgctgagc aaattcaaaa gaaactacct caggcgatac tacttccaac
    22321 cctgccttct gcttacgaat gtggtctttt ggggcaaagt ttggcaccag aaaatgtaga
    22381 cgcctttgtc cctcaatatc tcaagagagt ggaagctgaa gaaaactggc tcaaagataa
    22441 tgagataaaa gatgatagtc actacgttaa gcgaatctaa tatgaaaaca gttgaagaac
    22501 aagcaaaaaa aatttatcag cttttggaaa tggtttatgg cacgtctcct tggactttag
    22561 aacaagtatt aatcgatata cggcgggatc aaacagatta tttcctacta tatgatcatg
    22621 ataaattgct aggtttttta gctattcaag atttagctgg tgaagttgaa atgactcaaa
    22681 ttgctatact acctagtcat caaggacttg ggctagcgtc tcaattgatg acgcatttag
    22741 atagcattga aagtgacatt tttttagagg ttagagaatc taatcataga gcacagggtt
    22801 tatatcagaa gtttggtttt aaacttattg gcaaaagacc agactactat agaaatccta
    22861 tagaaacagc tttattgatg aaacgggaag gaaaacatga cagataggta tattttagca
    22921 gttgagagtt cttgtgatga gaccagtgta gcgattttaa aaaacgaaag taccctctta
    22981 agtaatgtca ttgcaagtca ggttgagagc cacaaacgtt ttggtggtgt ggtgccagaa
    23041 gtagctagta gacaccatgt cgaagtgatt acgacttgct ttgaggatgc tttgcaagaa
    23101 gctggcatca gtgcttcaga tttatcagca gttgcggtaa cttatggccc tggacttgtt
    23161 ggagccttgt tagttggatt agcagctgcg aaggcttttg cttgggcaaa tcatttacct
    23221 ttgattccgg ttaatcatat ggcaggtcac ttaatggcag cgcgtgaaca aaagccttta
    23281 gtttatcctt taatagcact gttggtttca ggaggacaca cagaattggt ttatgtccca
    23341 gagccagggg attatcatat tattggcgag acgcgtgacg acgctgttgg tgaggcttat
    23401 gataaagttg ggcgtgtgat gggcttaacc tatccggcag gacgtgaaat tgaccagttg
    23461 gctcataaag gtcaagatac ttatcatttc cctcgtgcta tgataacaga agaccactta
    23521 gaattttctt tttcaggact caagtccgct tttatcaatc ttcatcataa tgctaagcaa
    23581 aaaggcgatg aactgattct agaagatttg tgtgcatctt ttcaagcagc tgttctagat
    23641 attttattgg ccaaaacaaa aaaagctctg agcagatacc ctgcaaagat gcttgtagtg
    23701 gctggtggtg tagcggcaaa tcaaggatta cgagatagat tagcacaaga aattacacat
    23761 atagaagttg tgattcctaa actaagactt tgtggagata acgcgggtat gattgcttta
    23821 gcagcagcta ttgagtatga caaacagcat tttgctaata tgagcctaaa cgcgaagcct
    23881 agtttagctt ttgaccagtt tccagacagt tttgtaataa actagtcaca cttgctagtt
    23941 agtaagattt ataatggata agaatctccc tttgaaaaaa caacccggta tcttggcata
    24001 ccgggttgtc atgatgataa gtaattggct tctatggtta ctaataattc agataattac
    24061 ctgcaaaata attgtctgcc taaggtgaag taagcttacc agctagcttt agtgacgcca
    24121 ggaagctgtc ctttgtgagc taagtcacgg aaattgatac ggctaacacc aaacttacgc
    24181 atgtaagcat gaggacggcc gtcaattttg tcgcggttct tcaagcggtt tggatttgaa
    24241 tcacgaggca atttgcgcag tgattcataa tcgcccttcg cttttaagtc acgacgaagg
    24301 tcagcgtact gttcaatcag ttggagctgc ttttggtatt tagcaatttt tgactttttt
    24361 gccattgctt tctccttctt aatatttaat aacctcagag tcgttatcag cgccctgaat
    24421 ccacttaact ggtatttaac tggttaagtg gatatataat cattatacaa taaataaaag
    24481 caaaagcaac taaaaaaatt aactagttat cttttttagc cataatatca aaaaactcca
    24541 catctaagtg tgggagtcag gtattaataa ctgtttaaag gtgtttaatg agtgtcgctt
    24601 tggcaacttt gagattgaac ttttaagtca aataaatcag caggaacgta gtgtttggtt
    24661 gagaccaaga tgtctttacc attgtagaga gtttcttgga ttaaaataaa agcactattt
    24721 tctgatagag tatacttagt tgctgtgtaa ttgccagtct tggtatagcc gatttggttg
    24781 caggaatgag aagctttacc agattcatag attttctctt ctaagaaagt aagaaggggc
    24841 tcttcttgat tgagatttat agcatattta gaaataacct caatagggat aaaagataaa
    24901 ctataagcaa tagctttatt ttggctatga taccagcgat caacaataat cacaacagga
    24961 gtttcttgtt ttagggatgc tgtaatggca acagtgggca cttcaatccg atattccaat
    25021 tcaacctttg tgatgtcaga tgataggctt gcatatatgg ggtgttgaag atattcgaaa
    25081 ccttggtgat atttagtttc aggagtcttg agtataaaat ttccctttcc tctgatattt
    25141 tttatgagat gatcctcttg gaggagggct agggatttac gtagtgtcat ccgactgaca
    25201 ttgagctgtc tagcaagttc aggttctgat ggcagttgca tgccaggact atatgtacca
    25261 tcttgaataa gttgaaaaat agtattatat acttgtacat gtttaagttt tttgagtttt
    25321 ttggttaagt cgttagtaga catatcactt acctcattaa taatctttag tggagtttaa
    25381 cttgtctaaa aactatttta gcactaacag tattccttga ttagaattgt acccatctat
    25441 agtgttagta tctcacactc aggtactttt tgtcaaaaaa gagatgaaaa acaccttaaa
    25501 aaagcgttta caaaataggg tgtgtgtgat actatacaag tgtatcaaaa attagacaag
    25561 taaaatacag gaggttttta acatgcaaaa ttattcaggt gaagtcggat tgcaatatca
    25621 cctacagatt cgtccaggtg atgtaggtcg ctatgttatc atgccaggtg atccaaaacg
    25681 ttgtgcaaaa attgcagaac attttgataa tgcagttctt gtagcagaca gccgtgaata
    25741 tgttacgtat acaggtacat taaacggtga aaaagtcagt gttacttcaa ctggtattgg
    25801 tggtccatca gcgtctattg cgatggaaga attgaaactc tgtggcgctg atacctttat
    25861 ccgtgttgga acatgtggtg gtattgacct tgatgttaaa ggcggagaca tcgttattgc
    25921 aactggagct atccgtatgg aaggtaccag taaagaatat gctccgattg aatttcctgc
    25981 tgtagctgac ttggaagtga caaatgctct agttaatgca gctaaaaagc ttggttatac
    26041 aagtcacgca ggagtagttc aatgtaaaga tgcattttac gggcaacatg agccagaacg
    26101 tatgccagtt agttatgaat tattaaataa atgggaagca tggaaacgtc tgggaacaaa
    26161 agcgtctgaa atggaatctg cagctctttt tgtagcagca agtcatctag gagttcgttg
    26221 tggatcagat ttccttgttg ttggtaatca agaacgcaac gctttgggta tggataaccc
    26281 aatggctcat gatacagaag cagctattca agttgctgtt gaagcactcc gcacacttat
    26341 tgaaaatgat aaatcacaat aagcgaggtt ttggaagtca tcatgcaatt tatttatagt
    26401 attattggta ttttattgat attaggaatt gtgtatgcaa tttctttcaa tcgtaagagt
    26461 gtttctctaa gtttaattgg aaaagctctt atcgttcaat tcattattgc gctaatctta
    26521 gtacgtatcc cactaggcca acaaattgtt agtgttgttt caactggagt tactagcgta
    26581 atcaactgtg gtcaagctgg tttaaatttt gtgtttgggt cattagcaga tagtggcgca
    26641 aaaactggtt ttattttcgc tattcaaacg cttggtaata ttgttttctt atctgcccta
    26701 gttagtctac tttattatgt gggaatcctt ggatttgtag taaaatggat aggtaagggc
    26761 gttggtaaaa ttatgaaatc ctcagaggtt gagagttttg ttgccgtagc taatatgttt
    26821 cttggtcaaa cagacagtcc aattttggtt agcaaatacc taggtcgtat gactgatagt
    26881 gagataatgg ttgtgttggt atcaggtatg ggaagtatgt cagtttctat tcttggtggc
    26941 tatattgcat taggcattcc aatggaatat ctcttgattg cttcaacaat ggttcctatt
    27001 ggcagtattc tcattgctaa aatcttattg cctcaaacag aacctgttca aaaaattgat
    27061 gacattaaga tggataataa aggtaataac gccaatgtga ttgatgcaat cgctgagggt
    27121 gcaagcacag gtgcacaaat ggctttctca attggggcta gtttgattgc ctttgttggt
    27181 ttagtttctt tgattaatat gatgttaagt ggattgggaa tccgcttaga acaaatcttt
    27241 tcatatgttt ttgctccatt tggttttctt atgggatttg accacaaaaa cattcttcta
    27301 gaaggaaacc ttcttggaag taagttgatt ttaaatgagt ttgtttcgtt ccaacaattg
    27361 ggtcacctaa tcaaatcttt agattatcgt acagcattgg tagcaactat ttcactctgt
    27421 ggttttgcta atttatcaag tttaggtatt tgtgtttcag gtattgctgt tctttgcccg
    27481 gagaaacgta gcaccctagc tcgacttgtt ttccgtgcaa tgattggtgg tattgctgta
    27541 agtatgctta gcgcctttat cgtcggtatt gtaactctat tctaaacgtt gacaaaagaa
    27601 agaaggatag taacgtggaa gtaaaagata ttttaaaaac ggtagaccat actttgctag
    27661 caacaacagc aacgtggcca gaaatccaaa caattttaga tgatgccatg gcttatgaaa
    27721 cagcttcagc atgtattcca gcttcttacg tcaaaaaagc agcagaatac gtttcaggta
    27781 aattagctat ttgtactgtt attgggttcc caaatggcta tagtacaact gcagcgaagg
    27841 tttttgaatg tgaagatgct attcaaaatg gtgctgatga aattgacatg gtcattaatt
    27901 tgacagacgt taaaaatggg gattttgata ctgttgaaga agaaattcgt caaatcaaag
    27961 ctaaatgtca agaccatatc ttaaaagtta tcgttgagac atgtcaatta actaaagaag
    28021 aacttatcga actttgtgga gttgtcacac gttcaggtgc agactttatt aaaacctcta
    28081 ctggtttttc gacagcaggt gctacatttg aagatgttga agtgatggca aaatatgtcg
    28141 gcgaaggtgt taaaattaag gcagcaggtg gaatctcatc attggaagat gctgaaacat
    28201 ttattgcttt aggagcttca cgcttgggta ctagccgtat catcaagatt gttaagaacg
    28261 aagctataaa aaccgatagc tattaactta atcataccga tttagcttga gactaaaaca
    28321 aaaaactcat gtaataggag ttaaaaagcc tactatctta gtgataatag gcttttttag
    28381 ctatgaagac ttccctgcga tccatcctga acaaagagct gaggtaatat taaaccctcc
    28441 ggtgtgagca ttaatatcta agacttcccc agcaaaatac agcccaggaa ctttcttact
    28501 ttccaaggtt ttaggattaa tttcttttaa atccacgcct cctttggtca caaaagattt
    28561 agccaaagac atttttcctg taatgggtat ctggaggtgt ttgagtttat caagcaattc
    28621 cttctcttgt tttggagaga gttgtttgac cttttcggga taatcttctg ataaaaaatc
    28681 agctactcgt tcaggcagta ggcctttaag agcatttttg atgtttttat cacgttgatc
    28741 actcagataa gcggttaagt catctgttga taagtgtggc aaaaaatcta gctctgctat
    28801 ttccccacct ttcacaaaag aagacaaacg gagagctgca ggacctgaaa ggccaaagtg
    28861 agtgaagagt aagtcgtgtg tgatgacgtg tttgtcataa ctaagagtaa cgtcgtctag
    28921 ggaaatacct tgcaagactt tgtgaggaaa atcagttaac agaggactct cagcagcttc
    28981 taaatcagtc acggtcagtt taaaatgccg tgcaatatca tgtccaaaac ctgttgaacc
    29041 cgttgaagga taggccttgc ctcctgtggt cacaatcaat ttatgacagc taaaagtctg
    29101 gtctgcagat ttgaggtaga agaggtcatc ttgtttttta acggaaacca cttcagtgct
    29161 ggttagtacc ttgcctccaa gagccttgat ttttttctcc agagcatcaa tgattgtcct
    29221 agatttatct gttgtgggga acatccgtcc atggtcttct tctttgagtt tgacgccgtt
    29281 ttcctcgaaa aaggcaatga tgtcatggtt gtcaaactgg gaaaaaacac tatataaaaa
    29341 gcggccattt ccaggaatgc ctgccataag gtcatcaagg ttaccactat tagtgacatt
    29401 acagcgtcca ccaccggttc ccgctaattt tttacctaag cggcgatttt tttcaattaa
    29461 gagagtttta tagccatagt agctgctgga gatggcggcc atcatgccag cagggccacc
    29521 tccaataata atcgtatcgt attgagtcat agccttattt tatcataaaa gcggggggtt
    29581 taatagtcag gtaaatgaac taacctggca taccgttttc tattcatcta acaatgtttt
    29641 caagaaataa ttgatgcctt tagcacgact gaaatgaggt caaattgaca gaatactcct
    29701 attatggtac tatttttaat aagaatattc agtgatagtg ataaagagga aaatatggga
    29761 attgaaaaga cagttagtga actagctgat attttgggag tgagtcgtca ggccgtcaat
    29821 aatcgtgtta agtctttacc cgaagaagat cttgacaaaa atgaaaaagg cgttactgtg
    29881 gttaagcgca gtggtcttgt taagctggaa gagatctata aaaaaacaat ttttgacgat
    29941 gagcctatca gtgaagaaac gaaacaacgt gagcttttag agattcttgt ggatgagaag
    30001 aacactgaaa tcacgcgcct ttatgagcaa ctcaaagcca aagatgctca acttgcctca
    30061 aaagatgagc aaatgcgtgt taaagacgtg cagatcgctg aaaaagataa acaattggat
    30121 cagcagcaac aattaactgc taaagccatg gctgataaag aaaccctaaa attagaattg
    30181 gaagaagcta aagcagaagc caaccaagct cgcttacaag tcgaagaagt tcaggctgaa
    30241 gttggcccta aaaaaggctt tttcacccgc ttgtttgcga aataatcaca aaaaatgacg
    30301 aaggccctat cagggtcttt tttggaggaa aacatgaaac acttagatac ttatattgcc
    30361 tttgatttag aatttaacac tgtcaatgac gttagtcatg tcattcaagt atcagcagtc
    30421 aaatatgacc accataaaga agttgacagc tttgacacct atgtttacac agatgttccc
    30481 ttacagagct ttatcaatgg cctaacagga atcacatcta acaagattgc tgcggcacca
    30541 aaagtagagg aggttatggc tgcgtttaaa aattttgtgg gagagttacc actgattggt
    30601 tataatgctc aaaaatctga cctgcctatt ctagctgaaa atggcttgga tttaagggac
    30661 caataccaga ttgacctctt tgatgaagct tacgatagac gtagcgcaga cttaaatggg
    30721 attgctaacc ttcgtctaca aacagtcgca acctttttag gcattaaggg tcgaggtcat
    30781 aatagcttag aagatgccag aatgacagca gcaatctata agtctttttt agaaacagat
    30841 acaaacaaag catatctcag ccagcaagaa gaggtaacca cagataaccc tttcgcagct
    30901 ttgggagact ttttcgacta aaggctctgc tatttttgaa aaataaaaaa atccctaggg
    30961 ttagcccttg cttataacgc taggtcatgt gataagctag ggacatatca agagggagtc
    31021 gagtgcacac gaatcgtttt tgatcactaa atgatagaaa gaaggtgtgg aaatgtctca
    31081 attctattcg actggagaac tggccaactt agctggcgtt tctattcgaa ctgtccaata
    31141 ctatgaccaa cgtgggattt tgattccgac agcattgaca gcaggtggac ggcgactata
    31201 tacggattct gatttagaac aattacgcat gatttgcttt ctgcgcgatt tgggattttc
    31261 cattgagcag attcgcaaag tcttagcaga ggaaaatgca gcccaggtgc tggaattgct
    31321 tctagttgat catattgcga ctgccaagga agacttggcc gcaaaagagc aacaagttga
    31381 catagctgtc aagatacttg acagacttcg aaaacaagac ccccagtcac tagattttct
    31441 gatggacatt tcgcttagca tgaaaaatca aaaagcttgg aaaaaattac aatggaagat
    31501 gggtatcagt atatttttag ccattgtttc atatgtagga cttattttac tatctaatta
    31561 tctgcaaaaa ttttggttag tttatgttgc tatgggaata tttctaccag gtttcagttg
    31621 gttggtaatt tattttagga agcaatttat ttatctctgt ccccattgtc atcagaaatt
    31681 tgaacctagc tttaatgaat ttgcccttgc aggccataca ccacgaacca gaaagttaac
    31741 ctgctcccat tgccatcaaa aatcgtattg cttggaactg gcaaaagagg acactgtctc
    31801 aagaggataa gcactaactc ataaaaaaac tagcctgaga tccttctcaa gctagttttt
    31861 gtttttttag aatggtaatt taccagcaaa agcgcctaat gttttcttag tcgcttcatc
    31921 aatttgcgta agggcatcat taatggcttg agtggtcatg tcttgaagcg tttctacatc
    31981 ttcaggatct acaacagctt ctttgaagtc aatcccaacc aattttttat cacccgtgaa
    32041 agtggcagtg acaaggtctt gagctgattt tccagtaaat tgcatagcag ccaaatccgc
    32101 ctgcttttgc tccatttgtt tctgaagctt ttgtgcttgc ttcatcatgt tttgcatgtt
    32161 catcataaga ctatactctc cttaatttaa tactaactat tataacatat aaaaagaggc
    32221 aataaaaaca atgtcagtta gtagactagc ctgtatcagt aagaaagtcg ctatggataa
    32281 gatggattag cagcaagggt ctggctaatg acagaataag tctagaaatg gattattttt
    32341 gtcatagact agtccgactt agtggttatc taattcacga tcttctttag cttgttgttc
    32401 taaaagaatt tgtgcgaagt caacccactt agtgtagtta ttttcggata attgttctga
    32461 aatcatcgct aactctcgtt gtggcaactg ataagagact cccttacttt ctaaggttaa
    32521 atcttcttcc attagggcgg ttaaactaac atcaaagata gcagcaatat cacttaatag
    32581 accagacttt ggagtatatt ttcctttttc ccactcactg attgaagaag agctttttcg
    32641 tcctaggaga ttagcaagtt ctaattgttc catgttcttt tttaatctaa ggtactttaa
    32701 attacttgca aaaaaagttt tcttggcgga catatttttc tccttttctt tatataaaat
    32761 atgataccat aaaagaggtt aaagcaaaag gtatcgtaaa agatggtcta aaaactctct
    32821 aaaaacttga cttcataaaa accgaagttt ataataaact taacatgatt aactaatctc
    32881 attttagtgt ggagggaaaa ttgtgaccaa atcaacttta aaagagttaa gaacaacaaa
    32941 gaaaatgaca cagcaggaat tgtctcactt aacaggcatt tctgtcagaa cgattgctag
    33001 gtatgaaaaa gatactaaaa agctaaggcg agcaaaatat gagaagctaa aaggtattgc
    33061 acaagcatta gcggtttccg ttgatgatat ttttttagga atagattcgg aatttatgaa
    33121 ctaagacgat cgttttctgt ttgctattag taagaccaat taacaaaaaa gtgcttagtt
    33181 atattgctaa gcacttttat ttgttaattt gatgctattg gcacaatcaa ttgagactga
    33241 ctgagatcga tagttaaagc atagttgctg ttgtcacgga cagtatgttc aaaatcggtt
    33301 gtgtataaga tgactcgaag ggtatctcct ttttctagat gatagatagt tggttgcaaa
    33361 ggaagtctaa cagtcatcca tttattatta ggaatagttt caattgacga caaattattg
    33421 cgattttgta aattcatgaa acctttcgaa ataacacggt aagaatcttc tctaaatggt
    33481 agctctttaa ggggttctct agagaaattt tgcccattgt caatacttga ttgggtgaga
    33541 gcaataggaa gatcgcccaa gcgttttttc ttaccatagt caaggatttg tgcagataga
    33601 agtcctttat tttcacttga tttcactttt aactgtagga caatttcacc gttaataggt
    33661 aagtcctctt ccagtaaaat atcaatcagg gcttgattgg ccttgccttt aaagagagct
    33721 gctttggacg cacggaaatc tttaccgtaa gctttaaatt catcttcacc atagtgatta
    33781 tcaattaaaa gcagttcctg accgaggtca agctctttga tggttgaggt tccaaagact
    33841 ttacgctcct gccagttttg tgggcaggtg ttatcctgcc agatcatttc aggaaggcta
    33901 aaatcattag ctaggcctaa gagtttttga cataaaaggg cattcatgct ttcgcgaaaa
    33961 tcaatggatt gccaattatg catgtaaaca tgctcacctt gatgtaaaaa gaggtgcttg
    34021 ttgattgtgg aaggaagcgc attgaaaatc tcatagacct gtcttggttt gacattccag
    34081 tcttgtaagc catgagtata aacgacatca cactttattt gatgagcatt ttttaagtaa
    34141 ttgcggtcgt gccaaaattg attataatca ccagattgtc ggtctaacgc ctgagactgt
    34201 tggtttagca attcttgata atgatcatta tggcgaagat aatcgccagc caataaatta
    34261 cgcgagtagg tgagctcagt taggacatct agatcctctc caggatagcc accaggacta
    34321 caaactaagc cattttcgcg gtagtagttg taccaagaag aaatagctga ttctgcaata
    34381 atcatggcta atccgtctac gcctgtagtg gctaaaccag tagacatagt tcccaagtag
    34441 gactttccag tcgtacaaac tagaccattt gcccaatcag ctctcacttg gtgggttttt
    34501 gagtggctgg tgtaggctgt ggctcttcca tttagccaat caataacggc tttaaagctt
    34561 tcaatttgag catagtctcc actcgtcata aagccagtgg aaccagcagt tcccacacct
    34621 gaaacataga tattagcaaa cccacgtgcg aggaaataat cgttgaggct ataggagttg
    34681 atgtagctaa agctttctag attttgacct ataggaagtt tgcaaggctg tgtctcaaga
    34741 ggaataaaat ccctgtcttc gacagtgatt tgtctccttt ttttaacgac taactctttt
    34801 tccatacgat aaagtttttt gtcgttggct acttcattga taccttgatg gtatggacta
    34861 ggtgtcatca aagttggtaa tggtttttga gattgaggac gaatgatatt aaccttaata
    34921 agatcaagtt ggccatcttg atcggtatca agaggtgctt caacgtaaac aacttctcga
    34981 atcagctgtg acgtgtcaaa ggtagctaat gatttaccgt tgaagaaatg gtaatcattg
    35041 tccatagtaa gcattccttg gctgaccaaa tcatcaatca gggtcatgcc agacttacaa
    35101 cgggtcgcta ataagtggtg gatatgcttg ataatttctg tttcgtcaaa gtggatagga
    35161 aaggccagtt tatcaaggaa agcatcggcc tctgtaaaat caactccagg aataaagcca
    35221 aggacttgca gggccagtaa atcaaaaacc tctttggata actcttgctc tgattgaaaa
    35281 aagctcaatg cgtccatgtc atttttggcg attagatgac ttaaaggata atcactatca
    35341 gggtagtgga agaagagttt acgtaaaaag ctttctaggt tagctttggc agttttttgc
    35401 agatccagat caaagcccaa ttcttttaat tcttcagccg ctctttctag actagttgga
    35461 atataggaaa attgattata gcgcatagga gtttcctttc tagtggctta gaaaacgata
    35521 ttatctttta atttatcaag attggtaatg ataatatgtt gtttatcaat gcgaatgatg
    35581 tttttttctt tcaactgttt tagaatacgg ctgacgctgc tagcggtaga aatgccacag
    35641 aatttaccga gttcctcatt agtaatgaca aaattaatta aaatttgacc atttggtagt
    35701 tcttcgccaa atagggtcat taagtcatat atttgagtag aaatggcacc gatacggccg
    35761 ttagttaaca tacattgcat ttttttcatg gatttttgaa gacgattatg gtaaaagtcc
    35821 ttcacatatc cttgtagttc aatatcttcg tttatatctt ttaaaaatgc tgagcggcga
    35881 accttgtaaa aactagcttt ctctgactcg atgcgaacat tataaggctc tcccatatct
    35941 tttgaataac cagtgttgag aacagaagta atttcaagtc ctgtaacata tctcaaatta
    36001 aattccattc catattttga aagaacactc tgtttcacaa tgccgtcttt taaaatataa
    36061 gtgaagtcct cttcgagact ttcaaaagtc aagtacttgt gataactttt ttcaatgata
    36121 ggaaagttat gattatcaat ataccgttgc aaaatgtgat gattcacgct tttctccaaa
    36181 tattctttta ccaatgtata atttgtatct tcaagagaaa ttaacatttg ctaatgcc
//
LOCUS       DAUGHY010000015        33767 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.015, whole genome shotgun sequence.
ACCESSION   DAUGHY010000015 DAUGHY010000000
VERSION     DAUGHY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33767
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.015"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            483..959
                     /locus_tag="VH752_000705"
     CDS             483..959
                     /locus_tag="VH752_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEQ8289076.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRPFFSAKFTYDQNNQLMDKSVTFY"
     gene            1017..2198
                     /locus_tag="VH752_000706"
     CDS             1017..2198
                     /locus_tag="VH752_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ8289077.1"
                     /translation="MPLIPEKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINHLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            2188..3435
                     /locus_tag="VH752_000707"
     CDS             2188..3435
                     /locus_tag="VH752_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEQ8289078.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDMEGLTEVAREKLLQAVSISPEPLVIFGLAEIDMSLQYFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLLYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLVTLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(3494..5146)
                     /gene="fbp54"
                     /locus_tag="VH752_000708"
     CDS             complement(3494..5146)
                     /gene="fbp54"
                     /locus_tag="VH752_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HEQ8289079.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRVQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKSLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFEPVLFSDSHATFETLSEMLDHFYQDKAERDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATENAELFRQKGELLTTYLSLVPNNQDSVILDN
                     YYTGEKIEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGKTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLS"
     gene            5500..6498
                     /locus_tag="VH752_000709"
     CDS             5500..6498
                     /locus_tag="VH752_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ8289080.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANRQNITIGIL
                     QFVTHEALDDIKRGIEDQLKKQMPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAATSKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKKLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVVDVPVKVVDIGQPLINKNVAAELGIAINREDFPSASFIEN"
     gene            6843..7712
                     /locus_tag="VH752_000710"
     CDS             6843..7712
                     /locus_tag="VH752_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289081.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCLPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERYFKGVRLTR"
     gene            7709..8467
                     /locus_tag="VH752_000711"
     CDS             7709..8467
                     /locus_tag="VH752_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289082.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDPKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQLTITDYYQLFD"
     gene            8671..10332
                     /locus_tag="VH752_000712"
     CDS             8671..10332
                     /locus_tag="VH752_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ8289083.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKGKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIVYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNYLKKDTFDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
     gene            10465..11250
                     /locus_tag="VH752_000713"
     CDS             10465..11250
                     /locus_tag="VH752_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEQ8289084.1"
                     /translation="MASIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRTAIERLLRHTNLIVVMPSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPETLLEGK
                     LGELAYWQGVFGQFDDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFATNEKAIADF
                     QAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            11280..11606
                     /locus_tag="VH752_000714"
     CDS             11280..11606
                     /locus_tag="VH752_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289085.1"
                     /translation="MKQLLAIILWLPKLIVKMFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            11652..13559
                     /locus_tag="VH752_000715"
     CDS             11652..13559
                     /locus_tag="VH752_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ8289086.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDKTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQDLTRLMTDYDLLTEHFRQQGGFTYESDIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPHGLDRYVGNYSRFMTLKAEKLVAEEKQFDKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKIERLDKPTSARKSAHMTFQADKPSGNVVLTVEKAAI
                     GYNQHVLSEPINLDVHKLDAIAIVGPNGIGKSTLIKSVIGQIPFIKGEVKYGANVEIG
                     YYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRLAT
                     KVLEITENGSTLYLGDYDYYLEKKAELEELARLAAGETVEETKEASATDYQLQKANQK
                     ERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDTAQLIAWQKEWDQLDQEQETLME
                     EWETIAEQIES"
     gene            13846..14814
                     /locus_tag="VH752_000716"
     CDS             13846..14814
                     /locus_tag="VH752_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEQ8289087.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
     gene            14873..15871
                     /locus_tag="VH752_000717"
     CDS             14873..15871
                     /locus_tag="VH752_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEQ8289088.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAAITGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            16056..17465
                     /locus_tag="VH752_000718"
     CDS             16056..17465
                     /locus_tag="VH752_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEQ8289089.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPTVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKG
                     RVHKEDVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIKAILEAAKP
                     AEAKAPAAKEEKVVDLPEGVEHKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            17792..19555
                     /gene="lpdA"
                     /locus_tag="VH752_000719"
     CDS             17792..19555
                     /gene="lpdA"
                     /locus_tag="VH752_000719"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEQ8289090.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVP
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAI
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            19864..19941
                     /locus_tag="VH752_000720"
                     /pseudo
     CDS             19864..19941
                     /locus_tag="VH752_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(20229..22646)
                     /gene="hylA"
                     /locus_tag="VH752_000721"
     CDS             complement(20229..22646)
                     /gene="hylA"
                     /locus_tag="VH752_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEQ8289091.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMMGQGTAIYADTLTSNSEPNN
                     TYFQTQTLTTTDSEKKVVQPQQKDYYTELLDQWNSIIAGNDAYVKTNPDMVTFHNKAE
                     KDAQNIIKSYQGPDHENRTYLWEHAKDYSASTNITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLNRENHQTTGKENKENWWVYEIGTPRAINNTLSLMYPY
                     FTQEEILKYTAPIEKFVPDPTRFRVRAANFSPFEASSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSPIEADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNAFYNVYDNLKTYHDIKLMKELL
                     SDTSVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTS
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKHPYCSYVNNQPVDLNNQLVDFTNTKSIFLESDDPAQNIGYYFFKPT
                     TLSISKALQTGKWQNIKADDKSPEAIKEVSNTFITIMQNHTQDGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYEKKATMFSNHNLSHQGFYSFPH
                     PVKQNQQ"
     gene            22879..23868
                     /locus_tag="VH752_000722"
     CDS             22879..23868
                     /locus_tag="VH752_000722"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ8289092.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFERLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            complement(23977..24768)
                     /locus_tag="VH752_000723"
     CDS             complement(23977..24768)
                     /locus_tag="VH752_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEQ8289093.1"
                     /translation="MTYTSLKSPENTDYLYNLNIAHLYGNLMNTYGDNGNVLMLKYVA
                     EKLGARVTVDIVSLNDAFDQDYYDLVFFGGGQDYEQTIVAKDLPSKKAALADYIAKNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLYDDEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTALKEKYGSAITLPSYDDILKQEVAEEYADIKRKAAFDKA"
     gene            complement(24768..26111)
                     /locus_tag="VH752_000724"
     CDS             complement(24768..26111)
                     /locus_tag="VH752_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEQ8289094.1"
                     /translation="MKMKTLLGIIAGKAAQSILTKLGRGSTYPGQLALACNKDILKDL
                     SKDYDIVVVTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKS
                     GRQIAVLEIDEASLPRITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPK
                     ATILANGDSPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNT
                     YANLGDFICLNCGFKRPQLDYRLTELTAITHQSSEFVIDGQNYKINVGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFTIGDKSCTLILIKNPVGASQALEMIQ
                     LADYPFSLSVLLNANYADGIDTSWIWDANFELITQMPITEINAGGVRHSEIARRLRVT
                     GFDDTKIKQAEKLEQIMEAIEKQESKHAYILATYTAMLEFRSLLADRHVVEKEMN"
     gene            26218..27069
                     /locus_tag="VH752_000725"
     CDS             26218..27069
                     /locus_tag="VH752_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEQ8289095.1"
                     /translation="MNNLSSIDIKFLLSLFADPWLLAVHLLDILIVAYLIYRFIKALT
                     GTKIMSLVQGVIFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEK
                     FGRSTQVFLQKQYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPL
                     NADISSQLLINIFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAI
                     GLSENSDALTIIVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSDSNVSLPWYKK
                     ILGGKSK"
     gene            27066..28022
                     /locus_tag="VH752_000726"
     CDS             27066..28022
                     /locus_tag="VH752_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEQ8289096.1"
                     /translation="MKRFLNSRPWLGMVSVFFAILLFLTAASSNHNNSSSQIYSPIET
                     YTHSLKDVPIDMKYDSDKYFISGYSYGAEVYLTSTNRIKLDSEVNNDTRNFKIVADLT
                     HSHPGTVNVNLRVENLPSGVTATVSPDKISVTIGKKESKVFPVRGSVDAKQIANGYEI
                     SKIETGVNKVEVTSDESTIALIDHVVAKLPDDQVLDRNYSSRVTLQAVSADGTILASA
                     IDPAKTNLSVAVKKITKSVPIRVEAVGMMDDSLSDIQYKLSKQTAVISGSREVLEDID
                     EIIAEVNISDVTKNTSKTVSLSSSQVSIEPSVVTVQLTTTKK"
     gene            28076..29431
                     /locus_tag="VH752_000727"
     CDS             28076..29431
                     /locus_tag="VH752_000727"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEQ8289097.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGTLVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     THPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIIFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKDRAMEVPAI
                     ANIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            29555..30196
                     /locus_tag="VH752_000728"
     CDS             29555..30196
                     /locus_tag="VH752_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289098.1"
                     /translation="MTKQQIEKLADLLPPFAATLYLDMIPEFQEGYASYVYQTQKEDI
                     QKRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQTLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLTD
                     MQEKLPSLSIMVSQESSKLFYQEQGFVLLAKGCKPGNENDSDVCQLVKYFSKP"
     gene            30259..31389
                     /locus_tag="VH752_000729"
     CDS             30259..31389
                     /locus_tag="VH752_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEQ8289099.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLKALIQE
                     FESYDIHDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTIANLKTAGFHNISIDLIY
                     ALPGQTLEQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKEEMMEEELFLGLRKKTGVSIQRFQEKFGISFEE
                     RYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            31399..32151
                     /locus_tag="VH752_000730"
     CDS             31399..32151
                     /locus_tag="VH752_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEQ8289100.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGG
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFKTDFVKKLHRVPKMPLLEQSIDRDYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCAFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQIISDGQLNAQAMIEWRAIKQTESEID"
     gene            32151..32915
                     /locus_tag="VH752_000731"
     CDS             32151..32915
                     /locus_tag="VH752_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEQ8289101.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQEKGIPYLLVTNN
                     TTRTPEMVQSMLAKQFNVETGIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYIEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            32915..33547
                     /locus_tag="VH752_000732"
     CDS             32915..33547
                     /locus_tag="VH752_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEQ8289102.1"
                     /translation="MVENTKLLCSWVWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDPVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
BASE COUNT        10425 a         5887 c         7096 g        10359 t
ORIGIN      
        1 gagataagag attgggaggt tgtgacaatc gagcggatat cagatgttat ggcagaactt
       61 tctgagtctg aataatttta gttcaccaaa gaatcaagaa agaaccattg gggcaaaatt
      121 ggggcataag tttagaactt ttgtcgtttc ttgtgtcgat acaataccgg tttaatacta
      181 acaaatgctt ttattatcga ctttgtaacc attattgttc cttgtgtttt ttcgggaata
      241 aactcaagaa gtgattaaat aaaacattaa agaaccttgt catatcaatt atcgttgata
      301 tgacaaggtt cttttgatag tttaaattga tgtttttcat tgtaggctct tatctacact
      361 tttttaaggc atctttagaa ggttttgagc gtgactaagt attcacactt tgactcataa
      421 aatgatagca tattttctag agattggtat aatagaaaca aagatggaaa ggaaagagat
      481 agatgactca attagcaacc atttgttaca ttgataatgg cgattctttg ctattattac
      541 accgcaataa aaaagaaaat gatgtccaca aaggcaagtg gatttcagtt gggggtaagc
      601 tcgaggctgg tgagacccca gatgagtgtg cccgtcgtga gattttagag gaaacccatt
      661 tgactgttac agagatggcc tttaaaggaa tcattacttt tccagaattt acccctggtc
      721 atgactggta tacctatgtt tttaaagtaa caggatttga aggagacctc atttcagatg
      781 aagagtcacg agaaggaacc ttggaatggg taccttacga tcaagtgttg gaaaagccaa
      841 cttgggaagg tgattatgac atttttaaat ggattttaga agaccgtcct tttttctctg
      901 ccaaatttac ctatgaccaa aacaaccaac tcatggataa atctgtgacc ttttattgat
      961 ttacccaggt taccagtaaa acttatcatt ttcccaaaga aaggaaccct atcttaatgc
     1021 ccttaatacc tgaaaagtct agaacagata gcttatttta caaatggttt ttaaataatc
     1081 aagctaccat ggctttagta ataacacttt tagccttttt aacgattttt gtttttacta
     1141 aaatctcttt tttatttatg ccagtgattt ctttttttgc ggtcatcatg ttgcccttgg
     1201 ttatttcgac cattctgtat tatctgacca aacccttagt tgaccttatt aatcatttgg
     1261 gcccgaatcg taccacctct atttttattg tttttggttt gattacctta ctttttgtct
     1321 gggcgatttc aggctttgtt cccatggtac agacccaact taccagtttt attgaggatt
     1381 tgcccaaata tgtgggcaag gtcaatgaag aagccaataa acttcttgaa aatgaatggt
     1441 tagtcagcta caagccccaa ctccaagaca tgcttaccca tacaagtcaa aaggctttgg
     1501 attacgcgca aagtttttca aaaaatgcta ttgactgggc tggtaatttt gctggtgcca
     1561 ttgctcggat taccgtggct attattattt cgccctttat tctcttttat tttttacgag
     1621 acagtagcca catgaaaaat ggccttgtga atgtcttacc tctcaaatta cgggtgccta
     1681 tggttcgagt cttgggagat attaacaagc aattatcagg ctatgtccaa gggcaggtta
     1741 cggttgcaat tgttgtcggc tttatgtttt ctatcatgtt tagccttgtg ggactgaaat
     1801 atgccatcac ttttgggatt attgctggct ttcttaacat gataccttac ttaggaagtt
     1861 tccttgccat gatacctgtt gtgattatgg ctatggtaca aggcccattt atgttggtta
     1921 aggtgcttgt tatctttatg attgagcaaa ccatcgaagg ccgttttgtg gcaccgcttg
     1981 ttcttggaaa taagctgagc attcatccga ttacgatcat gttcttattg ttgactgcag
     2041 gatcaatgtt tggcgtttgg ggtgtcttct tagtcatccc aatctacgct tctgtcaaag
     2101 ttgtgattaa agaattattt gactggtaca aaaaagttag cggtttgtat gacgaagaag
     2161 tattggtgat tgaggaggtt aaggaccatg ttaaatagtg aaaaaatgat tgcttccttg
     2221 gatcagcaag acctagcgca cgctgaaaaa tatttccaaa aagctttaaa agaagatgat
     2281 gcagatagtt taatcgcctt aggagaatac ttagaaagta taggtttttt gccacatgcc
     2341 aagcgtattt atttgcagtt agctgatgat tacccagaac tcaatataaa tttagctcag
     2401 attgcagcag aagacgacgc cattgaagaa gctttcttgt atttggataa ggtttcaaaa
     2461 gatagcccca attatttgtc agctctctta gtaatggctg acctctatga catggaagga
     2521 ctgacagagg tggcgcgtga aaaattgctt caagcagtga gcatcagccc agagccattg
     2581 gtcatttttg gtttggcaga aattgacatg tccttgcagt attttaaaga agctattgat
     2641 tactatgcac agttagacaa tcgtcaaatc cttgaattga caggtatttc cacttatcaa
     2701 cgcattggac gagcctatgc tagcttaggg aaatttgagg cagccatcga attcttggaa
     2761 aaggccgtgg ccattgaata cgaagatgaa actgtttttg aattggcgac cctcttgtat
     2821 gaccaagaaa attatcaaaa agctaatctt tactttaaac agttggaaac catcaatcca
     2881 gattatccag gttatgaata tggttatgct ttgagtcttc atgaagagca taagacgtca
     2941 gaagctttgc gcttggttca acagggccta cgtaaaaatg catttgacag tcaattatta
     3001 ttactagcat cgcagctttc ttatgaatta cacgataggc agaatgctga aaattacctg
     3061 cttcaagcaa aggaagtagc tgttgatgac gaagagattc tcatgcgttt agtgaccctt
     3121 tattttgatg ccgagcgttt tgaggaggtt attgctctta atcgtgagac gattgataat
     3181 gtcttaacca aatggaccat tgcaaaggct taccacgcct tggaacaaga agaagttgcc
     3241 ttggctcttt ataatgagat ttcggctgat ttggcagaaa atccggagtt tttacaggat
     3301 tatgcctatt tattaaggga gtttggccag tttcacaagg ctatccaaat ggctaccgct
     3361 tacctcagac aagtgcctga tgatgtgaat atgcaagact ttcttgacca tatcaagcac
     3421 caacaatcag aataaaaaaa cagttctcct aggggaaata actttcctag gagagctgtt
     3481 ttttagttgc ttatcaggat aatttcatag agagaatttt ggcttggtca ggagtaaccc
     3541 gtaaagtttt ttgaccagta taagtcacaa atcctggctt ggcgccacta ggtttgtgta
     3601 attttttagc ttcaatcata tcgacctgaa cgagatttga taacctagct ttcgaataat
     3661 aagcagctag ttcggcagca tcagttttaa cctcgtcact tgggtcaaga ttgtctttga
     3721 tgatgacatg acttccaggg atatccttag catggaacca gagttctcct tttttagcca
     3781 ttttaaaggt gagttcttca ttttgcaagt tattgcgtcc caccatgaga atggtttttc
     3841 catctgaagc caggtactgc tcaggctttt tacgtttatg gcgtttgtct ctttggcgac
     3901 ttttcaaaaa gccagcctga tataattctt cgcgaatatc ttcgatgtcg tcgatacttg
     3961 cttgagataa attgtagtcc acgctctcaa agtaagtaat actttgcttg gtatcggcaa
     4021 tcaaaccaga taagtgtttg acagcttctt ttagcttttg gtatttttta aaataacgtt
     4081 gagcattttg atttggtgtc agagccttgt ctaaggcaat ctcaattttt tcccctgtat
     4141 aataattgtc taaaatcaca gagtcttggt tgtttgggac cagactgagg taggtggtca
     4201 ataattctcc tttttgacgg aataattcag cattttcagt ggctagcagc tcagcttctt
     4261 gcttacttag cttgttacga tttttgtcca gttcagtttg cacacggtga atcaaatcac
     4321 tggcttgctg attgatacgg tcacgctcag ccttatcttg ataaaagtga tcaagcatct
     4381 ctgataaagt ctcaaacgtc gcatggctat ctgagaaaag aacaggctca aaagaagctg
     4441 tggtcagatt tgcctgagta ggtctagcaa aaaactcacg gaaccgtttt aatttgtctg
     4501 tggtcagaag ctcggccaac tcttttgcgg tgtctcgccc caaaccttga aaatgctgtt
     4561 gcaagctttt aactgttagt tcttgtgttt ggaggatttc aaatagagga acatctgtaa
     4621 ttgtaaaagg attaacagca gctgttttag ggggctcaat gtaggtagag ccaggtaaaa
     4681 tggtgcggta agagttttgt gaaaagccaa catgtttaat ggattcgatg attttatttt
     4741 ctgcacggtc caccaagatg atattgctgt gcttccccat gatttcaatg attagggttg
     4801 cctgaatagc atctccgatt tcatttttat tagatacctt gatttcgata atgcggtcat
     4861 tatcaatctg ttctaactgc tcaatgactg caccctggag gtatttacgc ataatcatgg
     4921 taaaggtatt aggtacctga ggattttgaa agtctgcttg ggtgatttgt acccttccaa
     4981 aaacaggatg ggcagacaat agcaatttat aattcttgcg atggttacga atggttaaga
     5041 caagttcccg ttcaaagggt tgattgacct tttggatacg accgtaaagc aagttctctt
     5101 ttaattcgtt ggttaaatgg tgtaagaaaa agccgtcaaa agacatggtt tctcctttct
     5161 gaattctaat ctcttcatta taccacaaat acttgactaa aaatatggac gaaagagctt
     5221 gcaatttttt tgatttaggt tataatgtta aaaaaacgag aaaggaagtc aatgatggta
     5281 aaaacacaac acttagtaaa ttggcaaggc tggcatagat aagttgtgtt tatgaattgg
     5341 catagatacg acttttagag tacttctaaa aagatatcta tgcgctgaca ttggatttca
     5401 caagggcgcc tagtaatcat tactaagtgc tttttctttt gagaagtatt ctcatcatca
     5461 acgttttaaa gagattgaca tgaaatgtga ggaattcaga tgaaaaacaa gagtttaata
     5521 gctaccctcc ttgttttaac tgtaatagtg ataggctctc tattatcaaa aggagtatca
     5581 aaggaaaacc gtgatttggc taaccgacaa aacattacaa ttggtatctt acaattcgtt
     5641 actcatgagg ctcttgatga tattaagcga ggtattgaag atcaactcaa aaagcagatg
     5701 cctcaaaaac aaaatgtagt gattaaggtt atgaatgctg agggggatca gagtaaaatc
     5761 caaacaatga gtaggcaatt agtgcaatca ggaagtgata tcgtgattgg tatcgcaacc
     5821 ccggcggcgc aaggcttagc agccaccagc aaggatatcc cagttgtcat gtcagcagta
     5881 tctgatccag taggatctag attagtgatg caattggatc aacctgaagc taatgtaact
     5941 ggtctatcta ataaagtgcc tgttaaacag acaattgatt taatgaaaaa gctgacacca
     6001 catgtcaaaa cagttggaat tttatatgca agtaatgagg ataattcctt atctcaagtt
     6061 aaagagttta ggcggttagc tcgcaaaaag ggctatcaag tcatttcata cgcggttcct
     6121 tctactaacg aagttcctgc cacgatgtca gttatgttag gtaaagtaga cgcggtattt
     6181 atcccccaag acaatactat agcaagtgca ttttcatcag tcatgacaac cagtaaggct
     6241 gcaaagattc ctgtctacac tagcgttgat cgaatggttg aaaaaggtgg tttagcagct
     6301 atttctcaaa atcagtacga tttgggagtt caaacagcta atcaggtgtt aaaattaatc
     6361 aaaggaaagc gtgttgtaga tgttcctgtc aaagttgtgg atattggtca accgctaatt
     6421 aataagaatg tagcagcaga attaggtatt gctattaaca gggaagattt tccttcagct
     6481 tcttttattg agaattaata caatgacatg tgatataaaa ggtcaagaaa aaaccataaa
     6541 aaacaccctc actgagatga atttggatgc caagtcagac aatcgtcttg aggatgtaag
     6601 ggttattttt atcattttta gttttaaaat gctctatttc agacacgtaa aaaatcttgc
     6661 cttttgagta agtttaaaca tgattctcga tttttttgtt ttctgtctta gtttcgttaa
     6721 agtgattgga aaaaagacta agccacggtg ctgaactaag tataaaacaa atctaaaaat
     6781 taatgtaaag aaagggttta tactggcgag agttacccgc taatataaat atgaggaaaa
     6841 atatgattat ttcatcagtt tcacaaggcc ttatatgggg agttttagga ttaggaatct
     6901 acctgacctt taggatttta aacttccctg acatgactac agaaggttct tttcctttag
     6961 gaggagctgt tgcggtcacc gctattagct tagggtggaa tccatttcta tcaaccttac
     7021 ttggtatgtt atcaggtgct ttagcaggtt ttttaacagg gctgttatat acgaaaggaa
     7081 aaatgccaac acttttagct ggaattttgg tgatgacgtc atgtaactcc attatgctaa
     7141 tggtaatggg aagagcaaac ttgggtttac atgatcataa acgtattcag gattgtttac
     7201 ctttttcaat tgatttaaat agccttttaa caggtctgat aactgttgtg attgttatta
     7261 gcgttttgat ctatttcctt tacactaatc taggtcaagc ttatatcgct acgggagaca
     7321 ataaagatat ggctaagagt tttggtatta acacggattg gatggaagtt atgggacttg
     7381 tggtttctaa tagtttaata gctttatcag gtgctctagt gagccaacaa gatggctatg
     7441 cagatgtctc aaaagggatt ggagttattg ttattggttt ggcgagcata attgttggtg
     7501 aagtactata ctcaactgga ctgactcttt tagaacgttt gattgctatt gtaataggct
     7561 cgattcttta tcaattttta atttcagtag ttattacgct aggttttaat acaagttatt
     7621 tgaaattaat tagtgctctt gttttagcac tgtgcctcat gataccagtg gtaaaagagc
     7681 gctactttaa gggagtgagg ttaacacgat gacaaaaatt attgaattga tcaatgcaac
     7741 agttgacgta gataatggtt ttgaagatgc taaaacaata ttggacaatg tgacactaac
     7801 tatctatgaa catgattttt taactatttt aggaggaaat ggagctggaa aatcaaccct
     7861 ttttaatgta attgcaggaa cgctaagttt gacacggggg caaattagga ttcttggtca
     7921 ggatgtgacg cattggccgg cagaaaaaag agctctttac ctatcgcgag tctttcaaga
     7981 tcccaaaatg ggaacagccc ctcggatgac agtggctgaa aatctcttaa tcgcacgtca
     8041 aagaggagaa aaacgctcct tggcttctcg aaaaataaca gagcacttag ctagttttga
     8101 ggatttggtt aaacgaacag gaaatggcct agaaaagcat ttagaaacac cagcaggtct
     8161 tttgtctggt ggacaaagac aggctttaag tttattaatg gcaacgttga aaaagccagc
     8221 tctcttatta ttagatgaac atacggctgc ccttgaccct aagacgagtc aatctttaat
     8281 gcaattgaca gatgagtttg tgacaaaaga tggcttaaca gctttgatga ttacccacca
     8341 tatggaagat gcattaactt atgggaatcg tctgattgta atgaaagatg gaaacattat
     8401 taaagattta aatcaaatgg aaaaagaaca actgacgatt actgactatt accaactatt
     8461 tgattaatca acgatctaaa tcattgacga ataataagtc agtgattttt tgatttccgt
     8521 actgaaaata atctgaaagt atcaaggcta tttagagaaa aaacgcctca aatatgatat
     8581 actatagaag atagtagcat agctacataa cagtaattcg acttacgttg taaggaccgt
     8641 ttgtaataat aaaatattta aaggagtatc atgactgata ttaaaatgat tgccttggga
     8701 ggggtccgtg aatacggaaa aaacttctat ttggtagaaa ttaacgattc tatgtttatt
     8761 ttagacgcag ggctcaagta ccccgaaaat gagcaacttg gggtagattt agtcatcccc
     8821 aatttagatt atgtaattga aaacaaaggt aaagtacaag ggattttctt gagtcatggc
     8881 catgctgacg ccatcggagc ccttccttat ttgttagctg aagtatcagc tcctgtattt
     8941 ggatctgaat tgactattga attagccaag ctatttgtga aaagtaataa cagcactaag
     9001 aaatttaata attttcatgt ggtagacagt gatactgaaa ttgaatttaa agatggccta
     9061 gtttcgttct ttagaacaac gcattcaatc ccagaaagta tgggtattgt tattggaaca
     9121 gataaaggaa acattgttta taccggtgac ttcaaatttg atcaagcagc aagagaaggt
     9181 tatcagacgg atttgctacg tttagctgag attgggaaag aaggggttct agctctttta
     9241 tcagattcag tcaatgcgac aagcaacgat cagattgcca gcgaatctga agttggtgaa
     9301 gaaatggaca gtgttatctc tgatgctgat ggccgtgtca ttgtagctgc agtagcctca
     9361 aatcttgttc ggattcagca agtatttgat tccgccactg cccatgggcg tcgtgttgtt
     9421 ttaaccggaa cagatgcaga aaatattgtc agaacagctt tacgtctaga aaaattaatg
     9481 attacggatg agcgtctttt aattaaacct aaggatatgt ctaaattcga agaccatgaa
     9541 ttaatcatcc ttgaagctgg tcgtatgggt gagccaatta atagcttaca aaaaatggca
     9601 gcgggacgtc accgttatgt tcaaatcaaa gaaggagacc ttgtttatat tgtaactaca
     9661 ccaagtacgg ctaaagaagc tatggtggcg cgtgtagaaa acttgattta caaggccggt
     9721 ggttctgtta aattaatcac tcaaaactta cgcgtgtcag gccatgctaa tgggcgtgat
     9781 ttgcaactat tgatgaattt gttaaaaccc cagtatcttt ttccagtgca aggagaatat
     9841 cgtgatttag ctgcccatgc aaagctagct gaagaagttg gtatttttcc agagaatatt
     9901 catattctga agcgtggcga tatcatggtg cttaatgacg aaggttttct ccatgaagga
     9961 ggtgtacctg ccagtgatgt catgattgac ggtaatgcta tcggtgatgt gggtaatatc
    10021 gttttacgag accgtaaagt cttgtcagaa gatggtattt tcattgttgc tattactgtt
    10081 tctaaaaaag aaaaacgcat tatttctaag gctaaagtta atacgcgtgg atttgtgtat
    10141 gtcaagaaaa gtcatgatat tttaagagaa agtgcagaat tagttaatac aactgttggc
    10201 aattacttaa aaaaagatac ctttgactgg ggcgaattaa aagggaatgt cagagatgat
    10261 ttgtctaaat tcttatttga acaaaccaaa aggcgcccag cgattctgcc ggttgtgatg
    10321 gaagttcgtt agtgaaaata ggaaggttag gcaaaaactg cctaccttct ttgattttaa
    10381 caactggtaa accaggaaac tgttcaccca aaaatacagt agatttacgt gataggatta
    10441 ttttggtata aaggagtgta atgaatggca agtattgcaa ttgaatacca ttcggtagtt
    10501 ttaggaatgg agcgtaaggt aaatgtgatt tatcctgacc agtctgaaat ccctaaaaaa
    10561 gatcaagggg ataaggatat tcctgtttta tatttgttac atggtatggg aggcaatgaa
    10621 aactcttggc aaaaacgaac agctattgaa cgcttgttgc ggcataccaa tttgatagtc
    10681 gttatgcctt cgacagacct aggttggtat acagacacgg cttatggact taactattat
    10741 cgggcgttgt cacaagagtt accgcaggtc ttagcagctt tctttcctaa tatgacccag
    10801 aaacgagaaa aaacctttgt tgctgggctc tctatgggtg gttatggtgc ctttaagtgg
    10861 gcactcaaaa gcaatcggtt ttcttatgcg gcatcgtttt caggtgcttt ggatttttct
    10921 cctgagacct tgcttgaggg aaaactggga gaattagcct attggcaggg cgtttttggc
    10981 cagtttgatg accctgacct tgacaagcat taccttaaaa atatggtggc agaaagtgat
    11041 ggaaaaacca aattttatgc ttggtgtggc tatgaggatt ttctttttgc cactaatgaa
    11101 aaagctattg ctgattttca ggctcaaggt cttgacattg attatcataa aggtcatggt
    11161 aaacatgagt ggtactattg gaaccaacaa ttagaagttc tgttagagtg gttaccgata
    11221 aactaccaaa aagaagaaag gttatcttaa ccatttccta gtacaacatg aggtgacaaa
    11281 tgaaacaatt actggcgatc attttgtggc tgcctaagct aatagtgaaa atgttttggc
    11341 atcttatcaa agggttttta caaactatct tattagtaac gattattatt atagggctga
    11401 tgtactattc taatcacagt gattccgttt tagccaataa gatctcgata gttactgagc
    11461 aagtggtgca aatttttgat atcttaacac aaaagccatc tgcaaagacg cgtcatggga
    11521 gtggtaatag tcattccact ttccttgtta gcgctaaaga aggtaatcaa cgacttaaaa
    11581 agagttcgat ttcttttgga aactaggctg aaaatgtcct caaaagggca tttttttgtt
    11641 agaatgaaag tatgattatt ttacaaggaa acaagttaga acgctctttc tcaggagatg
    11701 ttctttttca aaacatatca ctgcaagttg atgaaagaga cagaattgca ttagtggggc
    11761 caaatggagc tggtaagtca accttactca agctattggt tggggaagaa accccaacga
    11821 gcggagaagt taatactaaa aaagacctaa ccttgtctta tctggctcaa aatagccgtt
    11881 ttgaatctga caaaacgata tatgaggaaa tgctcaaggt tttcgaagca ttacgacagg
    11941 atgaaaagcg cctgcgtcag atggaaatgg atatggcaac cgtttcaggt caagatttaa
    12001 cccgactgat gacagattat gatctgctta cagagcattt tcgtcagcaa ggcggcttta
    12061 cgtatgaatc tgacatcaaa gccattttaa atgggtttaa gtttgatgag agcatgtggc
    12121 agatgactat cgctgagctg tcaggaggtc aaaacactcg tctggccttg gctaaaatgc
    12181 ttttagaaaa gccagaacta ttggtccttg atgagccaac aaaccactta gatattgaaa
    12241 ccattgcctg gttagagaac tacttggcca attatcaagg agctctaatc attgtgagtc
    12301 acgaccgtta ttttttggat aaggtggcaa cagtgacctt ggatttaact cctcatggat
    12361 tggatcgcta cgttggcaat tattctcgtt ttatgacttt aaaagctgaa aaattagtag
    12421 ccgaagaaaa gcagtttgac aaacagcaaa aagaaattgc caagttagaa gattttgttc
    12481 aaaaaaatat tgtcagggct tcaactacaa aacgtgctca ggcgagacgt aagcaattgg
    12541 aaaaaataga acgcttagac aaaccgacta gtgctagaaa atcagctcac atgacttttc
    12601 aggcagataa gccgtctggc aatgtggtgt tgacggttga aaaagctgct attggttata
    12661 accaacatgt tttatctgag cctattaacc tagacgttca caaattagat gcgattgcta
    12721 ttgttggtcc aaatggtatt ggtaaatcca ctctgattaa atctgttatt ggacagattc
    12781 ctttcatcaa aggagaagtg aaatacggtg ccaatgttga aataggttac tatgaccaaa
    12841 cccaatcaca tttgacgtct agcaacacag ttttggaaga attatggcag gatttttcaa
    12901 ctacgccaga agtcgatatc agaaaccgat taggggcttt tctcttttct ggagatgatg
    12961 tcaaaaaatc ggtggccatg ctgtcaggtg gagaaaaagc ccgtctgctt cttgcgaagt
    13021 tatctatgga aaacaataat tttcttgttc tggatgagcc gactaaccac ctagacattg
    13081 acagtaaaga agtgctagag aatgccctca ttgactttga cggcaccttg ctttttgtca
    13141 gccacgaccg ctatttcatt aatcgtttgg caactaaggt tctggaaatt accgaaaatg
    13201 gctcaaccct ttacttaggt gactatgatt attaccttga gaaaaaggca gagctagaag
    13261 aactagcccg cctagcagca ggagagacag ttgaggaaac aaaagaagcc tctgccactg
    13321 attatcagct acaaaaagct aatcaaaaag agcgtcgtcg cttgacccgc cgatatgagg
    13381 agattgaagc aagacttgaa accattgaag agcgcattgg tgctattcaa gaagacatgc
    13441 atgcctccaa tgatacggcg cagttaatcg cttggcaaaa ggaatgggac caattggatc
    13501 aagaacaaga aactcttatg gaagaatggg aaaccatcgc tgaacaaata gaatcataaa
    13561 aataagcttt agcagttttg ctaagcttat ttttatcgct ttgttacttg tttgaaggtg
    13621 tgaaagaggt tttaaaatga tgtgtttttt ttatgtcaag gtcggtaaaa cgtgaaactg
    13681 ttatatagta tttttaaatt ttttagtaca gttttgtttg taatttcaca aactattgca
    13741 tttctaaaaa accaaataag gtaaggtaga aagcgattta attatttgca aattgtgaaa
    13801 aaaagtttta tcataaaaag gtaatatgat aatatggagg ccgaaatggt aacagtttct
    13861 aaagaacaac atttggatat gttccttaaa atggaacgta ttcgtgaatt tgattcacgt
    13921 attaataaac tcgtccgtcg tggatttgta caaggcatga ctcacttctc agttggtgaa
    13981 gaagcagcca atgttggagc ggtggctcac ttgtcttacg atgacattat tttttcaaat
    14041 caccgtggac atggacaatc aatcgctaaa gacatggacc ttaataaaat gatggctgag
    14101 cttgctggta aagcaactgg tgtttctaaa gggcgtggtg gctcaatgca cttggctgat
    14161 tttgaaaaag gaaactatgg taccaatggt atcgttggtg gtggttacgc tcttgcagtt
    14221 ggtgctgccc tcacccaaca atacaaagga actaacaata tagctgttgc tttctcaggt
    14281 gatggcgcta ctaacgaagg atcattccac gagtctgtta atatggctgc aacatggaaa
    14341 cttcctgtta ttttcttcat catcaacaac cgttatggaa tttcaatgag catcaataat
    14401 gcaacaaata caccacactt atacacacgt gcagaagctt acggaattcc tggtttctac
    14461 tgtgaagatg gcaatgacgt gatggctgtc tatgaaacaa tgggcaaagc cgttgaacat
    14521 gttcgtggtg gaaatggccc agctattgtt gaggtagaat cataccgttg gtttggtcac
    14581 tcgactgcag atgctggtaa ataccgtact aaagaagaag tggatgagtg gaaagaaaaa
    14641 gatccaatga tcaaatatcg tacttatctg acaagcgaag gcattgccac tgatgacgaa
    14701 ttggatgcta ttcaagcaca agtcaaaaaa gaagttgacg atgcttatga atttgcacaa
    14761 aacagtccag atccagaact ttcagtagcc ttcgaagacg tttgggttga ctaagatcac
    14821 cttgtcagta agaagcaatc gtatttgaat aataaatgga gaaataaaaa tgatgtcaga
    14881 aacaaaatta atggctttgc gtgaagcagt caaccttgct atgactgagg aaatgcgcaa
    14941 ggacgaaaac attttcctta tgggagaaga cgtaggggtt tacggaggag acttcgggac
    15001 ttctgttgga atgattgaag aatttggtcc taaacgtgtt aaagataccc caatttcaga
    15061 agctgctatc tctggtgcag ctattggtgc agcgattaca ggactacgtc caattgtcga
    15121 cgttaccttc atggatttcc ttaccatcat gatggacgct atcgtgaata atggtgctaa
    15181 aaataattac atgttcggtg gaggccttat tacccctgta accttccgtg tggcatcagg
    15241 ttctggtatt ggttccgctg cgcaacactc acaatctctt gaagcatggt tgactcacat
    15301 tccaggaatc aaagttgttg ctcctggtaa tgccaacgat gctaaaggct tattaaaatc
    15361 agctatccgt gataataaca ttgttctttt catggaacca aaagcgcttt atggtaaaaa
    15421 agaagaagtt aaccaagatc ctgacttcta tatcccactt ggcaaaggtg acatcaaacg
    15481 tgaaggaact gatttgacaa ttgtgtcata tggtcgtatg ttggagcgtg tccttcaagc
    15541 ggctgaagaa gttgcagcgg atggtattaa cgttgaagtt gttgacccac gtaccctcat
    15601 cccacttgat aaagagttga ttattgaatc tgttaagaaa acaggtaaat tgatgcttgt
    15661 caacgatgcc tacaagaccg gtggattcat tggggaaatt gcaaccatga ttaccgaaag
    15721 tgaagctttt gattaccttg atcatccaat cgttcgtctt gcaagtgaag atgttccagt
    15781 accttatgca cgtgtacttg aacaagcgat cttaccagac gttgagaaaa tcaaagctgc
    15841 cattgtgaaa atggcaaaca aaggcaacta gaagataagg caagtggtct gataaaggct
    15901 atgtttatca gtaacgtctc agctaaaaac tattttgaaa gtttttagaa gaactgagtt
    15961 gtcttatccc gtgtcgcggc acctcacctt tcgtatgttt tcgtagcaag gtgaagatgt
    16021 tgatggcgct tatcaaatcg tgaaaggatg tagaaatggc atttgaaatt attatgccaa
    16081 agctgggcgt tgacatgcaa gaaggcgaga tcatcgagtg gaaaaaacaa gaaggtgata
    16141 ccgtcaatga aggtgatatc cttcttgaaa tcatgtcaga caaaaccaat atggaacttg
    16201 aggcagaaga ctcaggtgtg ctattgaaaa ttgtccgtcc agctggtgat actgtacctg
    16261 taactgaagt tatcggttac attggtgcag agggtgaatc agtagatact attgcttcaa
    16321 gtgaaaaaac aacagaaatt ccagttcctg cttcagcaga tgctggacca acagttgctc
    16381 caaaagaaaa tgtggcaagt ccagctcctc aagtggcagc gacagctatt ccgcaaggaa
    16441 atggcggaaa agttcgtgct actccagctg cacgtaaggc agcagcagaa atgggcattg
    16501 accttggcca agttccagga acaggaccaa aaggacgtgt ccataaagaa gacgttgaaa
    16561 actttaaagg tgctcaaccg aaagcttcac cacttgctcg taaaattgca gcagacaaag
    16621 gtattgattt ggcaaccgta tcaggtactg gctttaacgg taaggtcatg aaagaagaca
    16681 tcaaggctat tcttgaagcg gctaaaccag ctgaagcaaa agcaccagca gctaaagaag
    16741 aaaaagttgt tgaccttcca gaaggtgtgg aacacaagcc aatgtcagct atgcgtaagg
    16801 caatctctaa aggtatgaca aactcttacc ttactgcgcc tacctttacg cttaactacg
    16861 atattgacat gacagagatg attgcccttc gtaagaagtt aattgaccca atcatggcta
    16921 aaacaggcct taaagtcagc tttacagact tgattggtat ggcagtggtt aaaaccttga
    16981 tgaaacctga gcatgagtac atgaatgctt ccctcatcaa tgatgctaat gatattgaat
    17041 tgcaccgctt tgtgaacctt ggtattgccg taggtcttga tgatggtctt atcgttcctg
    17101 ttatccatgg ggctgacaag atgagcttgt cagatttcgt gcttgcttca aaagatgtga
    17161 ttaaaaaagc gcaaactggt aagttgaaag cagccgaaat gtctggatca accttctcta
    17221 tcacaaactt gggaatgttt ggtacgaaga catttaaccc aattattaac caaccaaact
    17281 cagcaattct tggtgttgga gcaactatcc caactccaac ggttgtggat ggtgagattg
    17341 ttgcccgtcc aattatggca atgtgcctaa ccatcgacca ccgtttagtt gatggtatga
    17401 acggtgcaaa attcatggtt gatctcaaga aattgatgga aaatccattt gaattgttaa
    17461 tctgatcaat cagttttaaa caagaaaagc attcaataat aaaagggatt ttgctaagtt
    17521 tagacttggt gcccttttct caaaaatata gaaaggatgt agaacgagga gctctcatgc
    17581 aaatgagtga taagcaattg aatccgagcg aaaagctcgg ataaaaatgg taagagttgg
    17641 ctaatcttag cgtcgtagac gtctgaactt tcctcaattt ttgtttcgga aagtcctagt
    17701 cgtcctgacg tgggtgcgtc aacgaaatca aagatttctg acttaccgca aaaattcaat
    17761 cgctttctcc ttgctcttgg tatcttaaat tatggctgtt gaaattatta tgccaaaact
    17821 cggtgttgac atgcaagaag gtgaaatcat cgagtggaaa aaacaagaag gtgatactgt
    17881 caatgaaggc gatattcttc ttgaaatcat gtcagataaa accaatatgg aacttgaagc
    17941 agaagactca ggtgttcttc ttaaaattac acgtcaagca ggtgaaacag tacctgtaac
    18001 agaagttatc ggctatatcg gtgctgaagg tgaatctgtt gaggtttcta gcccagctgc
    18061 ttcagatgtg aatgttgctc gtacaacaga agatttagaa gctgctggac ttgaagtgcc
    18121 aaaagcacca gctcaagctg cttcagctgc accaaaagct gcacttgctg atgatgagta
    18181 tgacatcatc gttgttggtg gtggccctgc tggttattat gcagctattc gtggtgctca
    18241 acttggtggt aaaattgcca tcgttgagaa atctgaattt ggtggaactt gcttgaacgt
    18301 agggtgtatc ccaaccaaaa cttaccttaa aaatgctgaa atccttgatg gtattaagat
    18361 tgcagcagga cgtgggatta accttgcctc aactaactat accattgaca tggacaaaac
    18421 agttgacttt aaaaacaccg ttgttaaaac cttaacaggc ggcgttcaag gtcttctaaa
    18481 agctaataaa gtcactatct ttaatgggct tggtcaagtc aaccctgaca agactgtaac
    18541 cattgggtca caaaccatta agggtcgcaa cgttatcctt gctacaggtt ctaaagtatc
    18601 acgtatcaat atcccaggta ttgactctaa acttgtctta acgtcagatg atatccttga
    18661 ccttcgtgaa atgccaaaat cactagcagt tatgggcggt ggtgttgttg gtatcgagct
    18721 tggacttgtt tgggcatctt acggtgtgga tgttaccgtt attgaaatgg ctgaccgtat
    18781 tatcccagct atggataaag aagtctctct tgaacttcaa aaaattcttt ctaagaaagg
    18841 catgaagatc aaaacatctg ttggtgtctc tgaaattgtt gaagcaaata accaattgac
    18901 cttgaaactt aacaatggtg aagaagttgt cgctgaaaaa gctcttcttt ctatcggtcg
    18961 tgtaccacaa atgaacggtc tggaaaatct taaccttgaa atggatcgta accgtatcaa
    19021 agttaatgac taccaagaga catcaattcc aggtatctat gcgccaggtg acgttaacgg
    19081 aacgaaaatg cttgctcacg ctgcttaccg tatgggtgaa gtggctgcag aaaatgcgat
    19141 acatggcaac acaactcgta aagctaacct taaatacaca ccagcggctg tttacacaca
    19201 ccctgaagtg gcaatggttg gtttaactga agaacaagcg cgtgaacaat atggtgatgt
    19261 gcttattggt aaaaacagct ttactggtaa tggacgcgcg attgcttcaa atgaagcaca
    19321 tggttttgtt aaagttattg ctgatgccaa ataccacgaa atcttaggtg ttcatattat
    19381 tggtccagct gcagctgaga tgattaacga agcagctact attatggaat ctgaattaac
    19441 cgttgatgaa ttgttattat caattcatgg acacccaacc ttctctgaag tgatgtacga
    19501 agcctttgca gatgtgcttg gcgaagctat ccataaccca ccaaaacgta agtaaaacgt
    19561 ctttgtctta ttaactaatg aaaaatatca tcacataaca gatcacaaaa aagactagca
    19621 caactatgaa ctgcacccca aacgttagat ataaaaccct aacgtttggg gtgtttttgt
    19681 atgacattaa gctatgaaga taagctagac atttagcgct aaaaaaacga gagaactccg
    19741 tttgaaagaa gcatctctga aaagatgaag acaaaaaacc attataaaga atggacaaag
    19801 acgagctctg attagacttt cttttaagaa cggctaaatt gactcgttct acctactatt
    19861 atcatcgatt attgcaacaa taaacgtatc aaaataaaac gaaaaggact tagtcctcta
    19921 caacacagaa ctaaatcctt tacttcaaat agagtagcca acttttgggg ctcagtgcag
    19981 tttccagttt ttttgtcttt aataacgttt cattgttaac ccttactttc caataacagc
    20041 taaatcctta agtctttctt acgaaagggt tttgtgatat ggttaaaaga agcaactaaa
    20101 gctccaccta agaattctaa agaggaggac tgatacccag tatgcggtaa gcgtcgttgt
    20161 cttttgtggg gaattttatg agagttaaga gcattgtttt tagctgcaat tccttgatga
    20221 gcaagctttt attgttgatt ttgcttgaca ggatgaggaa aactataaaa gccttgatga
    20281 gaaagattat gatttgaaaa catcgttgct tttttttcat agtgaatgac gtgcatctgt
    20341 tgactatcat gatcgtagac agcggccagt ttgtcattgt tttctaacaa gtcgatatca
    20401 agcttgctaa tataggtttc aaattcttga cgagtcatat ttggaagcat catataggca
    20461 taacgatcgc catcttgagt atggttttgc atgatagtga taaaggtatt tgaaacttct
    20521 ttgatggctt ctggtgattt gtcatcagct tttatgtttt gccatttccc tgtttgaagt
    20581 gccttactta tgctaagtgt tgttggcttg aagaagtagt aaccaatatt ttgagcggga
    20641 tcatcacttt caaggaaaat acttttagtg tttgtaaaat caactagctg attattcaag
    20701 tcaacgggtt gattgttaac ataagaacag taagggtgct tttgattttc ttttcgttgc
    20761 tcaatagttg tatacgcctt gtgagatgac tggtttttga tattgctacc aacaaagatt
    20821 attttgtttc ctaagataaa ccacccttta ttgagagtga ggcttttatt ccaattagtg
    20881 aaggtcatag cagctagagc acttgtgtta ttaagttttt tacttgcaac aaaagcgtca
    20941 tcagagagac tagtcatgcc aacttgttga tagttcgttt taatattctc aggagttccc
    21001 tctagtggtt tttgctcagt ttctgtggtt ccaggtaagc ggtagggatt taccgttgcc
    21061 caatagtttt cactgtagtg tcctaaatcg ttattgtata ggtaaaacat tccatcagaa
    21121 gtaaaccagc catgaagatt ttcattattc atagcttcat aattttgagt tcgattcgaa
    21181 aacattgata gaccaaaagc aaaatcgtgt ttattattat atagtgccaa tttatccata
    21241 ctattgaaac tagctacgta actatcaagt ttttggactg gaacagaagt atcacttagc
    21301 agttctttca taagtttgat atcgtgatag gttttcaaat tatcatagac attgtaaaaa
    21361 gcattccctt gtgtgacgag tgtttttata cgtgttttaa gtgccaaacg gtgaggctct
    21421 tcagacatgt cagcaatacg taaaatagca cgaagtgctt caataccagc aacatgagat
    21481 tgagcattaa aacgactgat agaacgccct cgagtcatat ccatcatttc tccacgaacg
    21541 atgataggga aaaaagaatg gttaatccaa tgatagatag tagccatttt atccgcttct
    21601 ataggagact ttgttttttg aataatagga attaattgcg ataagccatc tataagaaca
    21661 ttaccgtaag ctccagtgta agcaatgcct tttttataaa gtggactttg agcgttagta
    21721 accacgtgat caattaaaga accgtcttgg taaaaaccat ttccttcgtc aactagcgtg
    21781 aaaactttct caattgcttt gattgtatca ctaatttcga gatcatcttt acgaagaata
    21841 ccggaaatga gtttaacacg tcccatatca attaaatttc cgctactggc ttcaaaaggt
    21901 gaaaaattgg cagcgcgaac cctaaaacga gtagggtcag gcacaaattt ttcgattgga
    21961 gctgtgtatt taaggatttc ttcttgagta aaataaggat acatcaagga taaggtatta
    22021 ttaatagcac gaggagttcc aatttcataa acccaccaat tttctttgtt ttcttttcca
    22081 gttgtttgat gattttcacg atttagatta taagcgtgtt catacatgaa ggccataccg
    22141 tcttttacaa tagcaatagc tttactatct tgataatagc atgattcagg attagtgatc
    22201 tgttttgcta ttttttcaat attgcggtaa gttttcgtga tattagtaga agcggaataa
    22261 tcctttgcat gttcccaaag gtaagttcta ttttcgtggt caggcccttg atagctttta
    22321 ataatgtttt gagcatcctt ttcagcttta ttatgaaaag tgaccatgtc aggattggtt
    22381 ttaacataag catcgttgcc tgcgataata ctgttccatt ggtctaacaa ttcagtatag
    22441 tagtcttttt gttgtggctg tactaccttt ttttcgctat ctgtagtagt gagcgtttgc
    22501 gtttgaaagt aagtattatt aggttctgaa tttgaagtca gtgtatcggc ataaatggca
    22561 gttccttggc ccatcatagc aaagctaagg aataggtttg aataaagtag taattgttta
    22621 tggtgtgtgc aaaaataagt attcactgat aaattcctcc aatataaaaa tgagataata
    22681 aaagtagatt ggtttactaa gtaaaaatat accacgttaa agtattttta taaaccctta
    22741 cataaataat ggctgagttt ttgtgcacta ataagtgtag gtactgaaga tgtcatacag
    22801 aataggacac ttgtctttta tatttgtttt cattatacta gagaggtaga ttacattttt
    22861 gaaaggattt tttctcctat gaaatacatc gttaacaaaa gtcacaatcc tgcctttaac
    22921 atcgctttgg aagcctatgc ttttagagaa ctagtggaag aagacgaatt gtttatcctt
    22981 tggatcaatg agccagctat tattattggc aaacatcaaa acaccattca agaaatcaat
    23041 aaagaataca ttgatgaaca tggtattcat gttgtgcgac gtttatctgg tggtggtgcg
    23101 gtttatcatg atttgaataa cttgaactac actatcattt caaataaaac agcggaaggc
    23161 gcatttgatt tcaaaacctt ctcacagcct gtgattgcga ctttagctga tttgggtgta
    23221 acagccaact ttacaggccg taatgatatt gaaattgatg gtaaaaaaat ctgcggtaat
    23281 gctcaagctt actacaaagg tcgcatgatg caccatggtt gccttttgtt tgatgtggat
    23341 atgaccgttc ttggcgatgc tcttaaggta agtaaggata agattgagtc taaaggagtg
    23401 aagtctgttc gtgctcgcgt gaccaatatt ttaaatgaat taccagaaaa aattaccgtt
    23461 gaggaatttt cagataaaat cttgaccaag atgaaagaaa cgtacccaga catgacagaa
    23521 tatgtcttgt cagaggatga gttggctaag attgaacaat ctgccaaaga gcaatttggt
    23581 agctgggatt ggacctacgg caaagcgcca gaatatacga ttgaacgcaa tgttcgttac
    23641 cctgcaggta aaatttcaac ctttgcaaat gttgaaaatt ctatcattaa aaaccttaaa
    23701 atctatggtg atttctttgg aatcaaagat gtgcaagaca ttgagaactt attaattggt
    23761 tgtaaatatg agtaccgtga tgtttttgaa cgtttaaaaa cgattgatac gacccagtac
    23821 ttctcacgca tgaccgttga agaagttgct aaagctattg tttcttagtg gttttaatca
    23881 cttaagaagc tctgtcagaa gaaaaaacta taaaactcct aagcgaaata tcgtgtcctg
    23941 cttaggagtt tttgtttgat gacatgaaag aagaacttaa gctttatcaa aggcagcttt
    24001 acgtttaatg tcggcatatt cttcagcgac ttcttgtttc agtatatcat catagctagg
    24061 tagtgtaata gctgatccgt acttttcttt gagagctgtt gttacgagac ggtaagcaag
    24121 gttcacgttg cgtgataaga taggaccatg gaaataactg ccgtagacgt ttttatagtg
    24181 aacaccttca gtttggtctt ccttattatt accattgcca taaaccactc gtccaagtgg
    24241 cttctcatca tcgtataaga aggtacgacc ttgatgattt tcaaagccat aataggtttc
    24301 gttaaattcg tcattatgaa ttttaatatc tccaataaag cgattttggt gctgattaag
    24361 ggtgtagtgt cccataattc caagtccatc aattttaacc ccattagctt ggacatagta
    24421 ttgccccagt aattggaagc caccacagat ggcaagcacc actttattct tagcgatata
    24481 atccgctaaa gcagcttttt tgctaggcaa atccttagca acgatggttt gctcatagtc
    24541 ttgtccacca ccaaaaaaaa caaggtcgta gtaatcttgg tcaaaggcat cattgaggga
    24601 aacaatatca acggtaactc tagctccaag tttttcagcg acatacttga gcatcaagac
    24661 attcccattg tcaccgtagg tattcatcag gtttccgtag agatgagcaa tgtttaggtt
    24721 atagaggtag tcagtgtttt ctggagattt tagtgaagtg taagtcatta gttcatctcc
    24781 ttttcaacaa catggcggtc ggctagcaag ctacggaatt ctaacattgc agtataggtt
    24841 gctaaaatat aagcatgttt gctttcttgt ttttcaatag cttccatgat ttgctcgagc
    24901 ttttcagctt gttttatctt agtgtcgtca aatcctgtga ctctaagtct tctggcaatt
    24961 tcagagtgac ggacgccgcc agcattaatt tctgtaatgg gcatttgggt aatgagttca
    25021 aaattagcat cccaaatcca gctggtatca atgccgtcag cgtaattagc atttaaaagc
    25081 acagataagc taaatgggta atcagctagc tgaatcattt caagcgcttg actggctcca
    25141 acaggattct tgataaggat aagggtgcat gatttatcac caatagtaaa agtttcttgt
    25201 cttccaaaaa ccgccttact tttgttaaag cccgccttga ttttctcggg agaaacaccg
    25261 aaaaattcag caacagaaac cgcagcaagc gcattgtaaa tattataaag accaccaaca
    25321 tttatcttgt agttttgtcc atcaataaca aattcagaac tttggtgagt aatagcggtg
    25381 agttcagtta atcgataatc caactgtggt ctcttgaagc cacaatttaa acaaataaag
    25441 tctcctagat tcgcataagt attgagacga tactgaagaa tatgttcgca ttttggacat
    25501 aaaatccctt ctgtgttgta atgagctagc tgcggagcat gcttagcagt atcaaaacca
    25561 taatactgaa ctggattaac tatgtcctta gaagagaaaa taggactatc tccattggct
    25621 agaatagttg cttttggagc atttcgagca ccatctacaa tcatctgata agtagtataa
    25681 atctctccgt agcggtccat ctgatcccta aaaatattag tataaacaaa aagactaggt
    25741 tttaagtaag tggtaattct aggaagacta gcttcgtcaa tttctaaaac agcaatttgc
    25801 cttccagatt tccctttttt agcagctaaa aaagtagaag taattccagt aatcatattg
    25861 gcaccacttg gatttgtgat tatctcgcca aatgcctctt tcaaaatacc gacagtaaga
    25921 gcagttgtta acgttttacc attagtacca gtgacgacca caatatcata gtctttagac
    25981 aaatccttta aaatatcttt attacaagct agagccaatt gaccaggata agtagatcct
    26041 cgccctagtt ttgttaaaat ggattgagca gctttgccag caataatgcc gagtaatgtc
    26101 ttcattttca tgcttatatt ttatcataag tttattgatt ttagataggg aaaaatcttt
    26161 gatcatgata tgatataata tagctaataa cttttttgat atagaaatga gagttgtatg
    26221 aataatttat ctagtatcga tattaaattt ttattaagct tatttgcgga tccttggctg
    26281 ctggcagtgc atcttttaga tattctaatt gttgcctatt tgatttatcg ttttattaaa
    26341 gcattgactg gaactaaaat catgtcttta gttcaagggg tcattttctt tttggttctt
    26401 cgttttattg cagagtggat tggctttacg acaatcacat acttaatgaa ccaagtcatt
    26461 acttatggtg taattgcagg agtagtcatt tttacccctg aaattcgggc aggactggag
    26521 aaatttggtc gttctacgca agttttttta caaaaacagt acgtcagcag tgaatctgct
    26581 ttggtcgatg ctttgattaa atctgtggcc tatatgggtc cacgcaagat aggtgcactg
    26641 attgctattg aacagactca aaccttacaa gaatatattg cgacaggaat tccgctgaat
    26701 gccgatattt caagtcaact actcatcaat atttttattc caaatacgcc gcttcatgat
    26761 ggagctgtta tcgttggtca gaataagatt gtggcagcct gtgcttactt gcctttgtca
    26821 gaatcaaagg caatttccaa agaatttggg acccgtcata gagcggctat tggattatct
    26881 gaaaactctg atgcactaac tatcattgtt tcagaagaaa caggagctat ttcagtaact
    26941 cgcaaaggcc aatttttaca cgatttaagc acagatgaat ttgaaacggt acttaggacc
    27001 tacctgatga gtgattcaaa tgttagttta ccatggtata agaaaattct aggagggaaa
    27061 tctaaatgaa acgattttta aatagtcgtc cttggttagg aatggtatca gtcttttttg
    27121 cgattctcct ttttttaact gcagcatcga gcaatcataa taattctagc tcgcaaattt
    27181 atagcccgat tgaaacctat acgcatagcc ttaaagatgt accaattgat atgaagtatg
    27241 atagtgacaa atattttatc agtgggtatt cttatggtgc ggaagtttat ctaacctcca
    27301 ctaatcgtat caaattagat tctgaagtga acaatgatac acgtaatttt aagattgtgg
    27361 cggatctaac acatagtcat ccaggtacag taaatgtcaa cctaagagta gaaaaccttc
    27421 cttctggggt gacagctacc gtatctccag ataaaatatc agtcacaatt ggcaaaaaag
    27481 aatctaaggt atttcctgtt agaggaagcg ttgatgctaa acaaattgca aatggttatg
    27541 aaattagcaa aattgaaacg ggggttaata aggttgaagt gaccagtgat gaatctacca
    27601 ttgctttaat tgatcacgtt gtggcaaagt tacctgatga tcaagtgtta gataggaatt
    27661 atagtagtcg agtgacctta caagctgtct cagctgatgg aactatttta gcaagtgcaa
    27721 ttgatcctgc aaaaacaaat ttatcagttg ctgtaaaaaa aataacgaaa tcagtcccga
    27781 ttagagttga ggcagtgggg atgatggatg atagcttatc agatattcaa tacaaattgt
    27841 caaaacaaac agctgttatc tcaggtagtc gagaggtctt agaagatatt gatgaaatta
    27901 ttgcagaagt taacatttct gatgtcacta aaaatactag taagaccgtg agtttgtctt
    27961 ctagtcaggt gtcaattgag ccgtcagtag tgaccgttca gttgacaacc accaaaaaat
    28021 aatggataat aactaggaaa attgaatgga ggaagccatt aggcttatat aaattatggg
    28081 aaaatatttt ggaacagatg gtgttcgtgg tgaagcaaat gttgagctga cacctgagtt
    28141 agcttttaaa ctgggccgat tcggtggtta tgttttaagt caacatgaga ccgaaagacc
    28201 taaagttttt gtggcgcgtg acacccgtat ttcaggagaa atgttggagt cagccttgat
    28261 tgctggtcta ctatctgttg gtattgaagt ttataaactt ggtgttttag cgacacctgg
    28321 tgtctcttat ttagttagaa ctgaaaaagc aagtgcaggt gtgatgattt ctgcaagtca
    28381 taaccctgcg cttgacaatg gcatcaaatt ctttgggaat gacgggttta agttagctga
    28441 tgaccaagaa ttggaaattg aagctctctt agatgctccg gaggacacgc ttcctcgccc
    28501 gagtgcagaa ggacttggaa ccttggtaga ttatccagaa ggacttcgta aatatgaaaa
    28561 gttcttggta acaacaggta ctgacttaag cggtatgaca gttgccttag acactgctaa
    28621 tggagctgct tcagtgtcag cacgtgatgt ctttttagat ttaaatgcag aaattgctgt
    28681 cattggcgaa aaaccaaatg gtcttaatat taatgacggt gttggttcaa cgcatccaga
    28741 acaacttcaa gagttagtta aggaaacagg agcagatctt ggtttagcct ttgacggcga
    28801 tagcgaccgt ctgattgctg tggacgaaac tggtgagatt gttgacggtg atcgtattat
    28861 ctttatcatt ggtaaatact tgtcagaaaa agggcttctt gctcacaata ccattgtcac
    28921 aacggtgatg tctaacctcg gttttcataa agctttagat aaacaaggta ttaataaagc
    28981 aattacagca gttggagacc gctatgtggt tgaagaaatg cgtagctcag gatacaatct
    29041 cggtggtgaa caatcaggcc atgtcattat catggactac aataccactg gagatggtca
    29101 gttgacagct atccaattag ccaaggtgat gaaggaaact gggaaatcac tttcggaatt
    29161 agctgctgaa gtaaccattt accctcaaaa actggtgaat attcgtgtgg aaaacagcat
    29221 gaaagaccgt gctatggaag ttccagctat tgcgaacatc attgcaaaaa tggaggatga
    29281 aatggctggt aatggtcgta ttctcgtgag accaagtggt acagaacctc ttcttcgtgt
    29341 catggcagag gcgccaacag atgccgaagt tgattattat gtcgatacta ttgctgatgt
    29401 cgtccgtaca gaaatcggat gcgataacta acgagttgag tacaaacaag aaaccttatt
    29461 tctaaggctt ctgtcaacaa ttggaaagag gcttggggcg tttagcccaa gtctcttttt
    29521 ataaattgct aaagtagaag agaaaggata tcggatgaca aaacagcaaa tcgaaaaact
    29581 agcggactta ctgcctcctt ttgcagccac actttactta gatatgattc ctgaatttca
    29641 ggaaggttat gcttcatatg tttatcaaac ccaaaaagaa gatattcaaa agcgccgcat
    29701 caagcagtta actaaaaatt ttgaacgatt actagatacc ccatcttttt atgtgatgcc
    29761 tgtgacaaga aaaacagcag agcaattgct agcagataaa ggcctagctt atcatttgtt
    29821 atcgacagtt gcagctaatc aagtgtttgc ggctgctgtt aacgaggatc agacgctttt
    29881 tcaagtgctt cgaaatggag caagagtagc ctttgtgatt cttcaagaaa agggggaatt
    29941 tctagaaatg actttaggga ttcttgaaac agtgacagaa aacgaggttc cagcagctat
    30001 tgtggaggct gtccagacat atcttctgac agatatgcaa gagaaactcc cttctttaag
    30061 cattatggtc agtcaagaat caagcaagct tttttaccaa gaacaagggt ttgttttgct
    30121 agctaagggt tgcaagccag gaaacgaaaa tgactctgac gtttgtcagc tcgtaaaata
    30181 cttttccaag ccatgaagaa tcaagggaaa tggtttattc atagaaacat tttatcatca
    30241 aagtggtata ataagaccat gtcaaaaaaa cctacttcag cctatgtgca cattcctttt
    30301 tgcacacaaa tttgttatta ttgcgatttt tcaaaagtct ttatccaaaa tcaacctgtt
    30361 gatgcttatt tgaaagcctt gattcaagaa ttcgaatcct atgacattca tgacttaaag
    30421 actctttata ttggaggagg tacgcctaca gctatcactg ctaaacagtt ggagtatctc
    30481 ctaaatcacc ttgaacgaaa cctcaattta gatgatcttg aagaattcac aattgaggca
    30541 aatcctggtg atttgacgcc agaaaaaatt gcggtgcttc agaggtcagc tgtcaatcgg
    30601 atttccttgg gggtgcagac ttttaataac aagcaattaa agcagattgg ccgtagccat
    30661 aatgaagaac aaatttatag caccattgcc aatttaaaaa ctgcaggctt tcacaatatt
    30721 tccattgatt taatttatgc cttgccaggt caaaccttag aacaagtcaa ggaaaatgtt
    30781 gctaaagctt tggcacttga tattccccat cttagtcttt atagccttat tttggaacat
    30841 cacaccgttt ttatgaacaa aatgcggcgt ggtaaactta acttaccaac ggaagacttg
    30901 gaagcagaaa tgtttgagta tattatttca gaaatggaag ctaatggttt tgagcattat
    30961 gaaatttcca attttaccaa accaggtttt gaaagtcgcc ataacttgat gtattgggat
    31021 aatgtggagt attttggatg tggtgcaggg gcttcaggtt accttaatgg cattcgttat
    31081 caaaatcggg tgcccatcca gcattatctt aaagcggttg aagcaggaaa cgctagacta
    31141 aatgaagaag tgctgcgcaa agaggaaatg atggaagagg aactcttttt gggcttacgt
    31201 aaaaagacag gagtctccat ccagcggttc caagaaaaat ttggtatttc ttttgaggag
    31261 cgttatggaa atattgttag ggagcttcaa aatcaaggac tgcttgtcaa agatgacgcc
    31321 tttgtcagaa tgactaaaaa gggtcttttc ttaggtgatt ctgttgctga gcggtttata
    31381 ttagattagg agagtattat gggattaagt tatcaggagg agttgacact tccttttgaa
    31441 ttatgtgatg tcaaatcaga tataaaattg ccccttttat tagactattg tttgatggtt
    31501 tctggtagac agtctgcgca attaggacga agtaacaaca atcttttagt tgattacaag
    31561 cttgtttgga ttgtaacgga ttatgagatc actattcatc gcttgccaca ttttcaagaa
    31621 accatcacca ttgaaacaaa agccctttcc tataataaat ttttttgtta tcgccaattt
    31681 tatatttatg atcaagaggg gggtctttta gtggatatct tagcctattt tgctttgtta
    31741 aacccagata cgcgaaaagt ggcaactatt ccagaagatt tagtagcgcc ttttaagact
    31801 gattttgtta aaaagctaca ccgtgttcct aaaatgcctc ttttagaaca atcaattgat
    31861 cgtgattatt atgtgcgtta ttttgatatt gatatgaatg gtcatgtcaa caacagtaaa
    31921 tatttagatt ggatgtatga tgtgttgggg tgtgcgtttt taaaaacgca tcagcctctt
    31981 aagatgactt tgaaatatgt taaagaagtc tcaccaggcg gtcaaattac ttccagttac
    32041 catttggacc aattaacttc ttaccatcaa atcatctcag atgggcagct gaatgcccaa
    32101 gccatgattg aatggcgagc gattaaacaa acagaaagcg agatagacta gtgccttata
    32161 agggttattt aattgattta gacggaacga tttaccaagg gaaaaaccgt attccagcag
    32221 gggagcggtt tatcaaacgc ctacaagaaa aaggcattcc ctatttatta gtgactaata
    32281 ataccacaag aacacccgaa atggtgcaaa gcatgctggc taaacagttt aatgttgaga
    32341 caggcattga gactatctac acagctacca tggcaaccgt tgattacatg aatgacatga
    32401 accgtggcaa gacagcttac gttattggag aaacagggct caaatcagct attgcagcag
    32461 caggttacat agaagaactt gagaatcccg cttacgtggt ggttggttta gacagtcagg
    32521 tgacctatga gatgttggct atagccacct tagctattca aaaaggtgcc ctctttattg
    32581 ggacaaatcc tgatttaaac attccaaccg aacgtggttt gatgccagga gcaggtgcat
    32641 tgaatgcctt actagaagct gccacacgtg taaaacctgt ttttattggc aagcccaacg
    32701 ctatcattat gaataaatca ctagaggtat tgggcattca gcgctctgaa gccgtcatgg
    32761 ttggcgataa ttacttgact gatatcatgg caggtattca aaatgatatt gcaacgatcc
    32821 tcgtgacaac aggctttacc agacctgaag aggtaccaac cttaccaatt cagccagatc
    32881 atgtcctatc tagtcttgat gagtggaggt tgtgatggta gaaaacacta aattactatg
    32941 tagctgggta tggttattag cgttagctat tctcataacg atctattcaa cgtggctgtg
    33001 gtatcctctt gaggtggacc atctaaagtt agaacaagtt gtttttatga gcaaggatgc
    33061 tatcttgcat aactataacg gactactaaa ttacctcacc aatccttttg taacaaggtt
    33121 ggaatttgct aattttcact catcggcaga cggactcaag cattttgcag atgtgaagtg
    33181 gctttttcac ctaactcaag tcgtttttct tggcctcctt tacccaaccc ttaagacttt
    33241 cacacaaagg ttaaaaacca aacgtttctg gctccttcaa aaacctttaa tactagcagc
    33301 tttgtttcct cttatgattg gtctaatggc tagtttcatt gggtttgaac atttttttac
    33361 tctttttcat caggtgcttt ttgtaggaga tagcagctgg ctctttgatc ccttgaaaga
    33421 tcctgtgatt tggatccttc cagaagtctt tttcttgcac tgtttccttt tctttatgat
    33481 agtttacgaa atcatcctgt ggagcttagt aggtttagca agatggcagc gcctaaatgt
    33541 agaatgataa aacaagcgtt ttgaaagcgc ttgtttttta gtttgcagtc agagtagaat
    33601 agaagtatca aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc
    33661 cttattttaa cttgctatgc tgttttgaat ggttccaaca agattatttt ataactttta
    33721 taacaaataa tcaaggagaa attcaaagaa atttatcagc cataaaa
//
LOCUS       DAUGHY010000016        32714 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.016, whole genome shotgun sequence.
ACCESSION   DAUGHY010000016 DAUGHY010000000
VERSION     DAUGHY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32714
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.016"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            18..98
                     /locus_tag="VH752_000733"
     tRNA            18..98
                     /locus_tag="VH752_000733"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Tyr,seq:gta)
     gene            105..175
                     /locus_tag="VH752_000734"
     tRNA            105..175
                     /locus_tag="VH752_000734"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137..139,aa:Trp,seq:cca)
     gene            187..259
                     /locus_tag="VH752_000735"
     tRNA            187..259
                     /locus_tag="VH752_000735"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:His,seq:gtg)
     gene            268..339
                     /locus_tag="VH752_000736"
     tRNA            268..339
                     /locus_tag="VH752_000736"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Gln,seq:ttg)
     gene            359..442
                     /locus_tag="VH752_000737"
     tRNA            359..442
                     /locus_tag="VH752_000737"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Leu,seq:caa)
     gene            702..887
                     /locus_tag="VH752_000738"
     CDS             702..887
                     /locus_tag="VH752_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289103.1"
                     /translation="MSFEIFTLIIGWKLVGYRVMECGSSFKQGDYRLVVHICLLNVRD
                     EMLSQQGHEDKADWPEP"
     gene            1525..1833
                     /locus_tag="VH752_000739"
     CDS             1525..1833
                     /locus_tag="VH752_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ8289104.1"
                     /translation="MKHRFQVKRGVSVYLEKRIPMCAGMGGGSSDAVTIRALNQLWLL
                     TLSRKDMMDIGIPIGSDVPYCLLSGCAQVTGKGEVVCRILGLLSSWVVLVKPDFGIST
                     "
     gene            1888..2100
                     /locus_tag="VH752_000740"
     CDS             1888..2100
                     /locus_tag="VH752_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ8289105.1"
                     /translation="MVAAIEAGNYNDGILTEMNNLLEDIFIAKRPFIQKIKEKTLQAG
                     AATTLMTGSGPTVFALCQTEKQTIIV"
     gene            2210..2653
                     /locus_tag="VH752_000741"
     CDS             2210..2653
                     /locus_tag="VH752_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEQ8289106.1"
                     /translation="MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHIL
                     MLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAK
                     PIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAEELEG"
     gene            2657..3376
                     /locus_tag="VH752_000742"
     CDS             2657..3376
                     /locus_tag="VH752_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289107.1"
                     /translation="MRYISVKNLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRHLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEVETDDEKGGHGHA"
     gene            3369..4184
                     /locus_tag="VH752_000743"
     CDS             3369..4184
                     /locus_tag="VH752_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ8289108.1"
                     /translation="MLDILSYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVLGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(4224..4607)
                     /locus_tag="VH752_000744"
     CDS             complement(4224..4607)
                     /locus_tag="VH752_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ8289109.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEVRYDLIDLQAALVAKLEQHLPISGVTSVSNDKELMDEGTHFHLHLIPRL
                     TNDSFWESITINQENWDLTPFLKHL"
     gene            complement(4658..5914)
                     /locus_tag="VH752_000745"
     CDS             complement(4658..5914)
                     /locus_tag="VH752_000745"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEQ8289110.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSEDSLNLVDMLVTAGISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LSNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            6006..8318
                     /locus_tag="VH752_000746"
     CDS             6006..8318
                     /locus_tag="VH752_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ8289111.1"
                     /translation="MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVI
                     FLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDL
                     LRTPVANDAISENIKKAIVSTEDEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLT
                     QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAG
                     VEEAARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQN
                     VLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYL
                     IKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLL
                     DDGTGTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPA
                     IDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLR
                     EKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYIVD
                     KITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLAN
                     ADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANA
                     INQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQK
                     GPGAMTYRFAIGGTDADYQKAWGNFGFRKN"
     gene            8582..12148
                     /gene="rpoB"
                     /locus_tag="VH752_000747"
     CDS             8582..12148
                     /gene="rpoB"
                     /locus_tag="VH752_000747"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ8289112.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDPDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGRVTNEIV
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQH
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
     gene            12239..15862
                     /gene="rpoC"
                     /locus_tag="VH752_000748"
     CDS             12239..15862
                     /gene="rpoC"
                     /locus_tag="VH752_000748"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEQ8289113.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDSQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTPDKYFLEPGQDIQEVIDGLDINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIVDAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVVPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAE"
     gene            16014..16379
                     /locus_tag="VH752_000749"
     CDS             16014..16379
                     /locus_tag="VH752_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEQ8289114.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVAYLCKLNL"
     gene            16472..17410
                     /gene="tadA"
                     /locus_tag="VH752_000750"
     CDS             16472..17410
                     /gene="tadA"
                     /locus_tag="VH752_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEQ8289115.1"
                     /translation="MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGQES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYARLLELGITKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII"
     gene            17394..18380
                     /locus_tag="VH752_000751"
     CDS             17394..18380
                     /locus_tag="VH752_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEQ8289116.1"
                     /translation="MKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYV
                     LKMEESLLKGQGLADMLSGLGFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRR
                     KTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLNHFPAFFIGFCSGLILL
                     FGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWSTLIGQGLDLMTILDI
                     MAIEKSSLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGVELE
                     IYAQESWEQFFSQLYQVTQLIQPAIFLVVAVTIVMIYAAILLPIYQNMGGIF"
     gene            18382..18708
                     /locus_tag="VH752_000752"
     CDS             18382..18708
                     /locus_tag="VH752_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEQ8289117.1"
                     /translation="MINQWNNLRHKKLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            18683..19111
                     /locus_tag="VH752_000753"
     CDS             18683..19111
                     /locus_tag="VH752_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEQ8289118.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            19116..19352
                     /locus_tag="VH752_000754"
     CDS             19116..19352
                     /locus_tag="VH752_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ8289119.1"
                     /translation="MIATGILFSIVILVLSSLQQSQAALTYYRKQQEKLNLALMAVQT
                     RTKEMTLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            19345..19779
                     /locus_tag="VH752_000755"
     CDS             19345..19779
                     /locus_tag="VH752_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEQ8289120.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYL
                     ARHDQDNWLLFSHQLREELSGARFCKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD"
     gene            19763..20089
                     /locus_tag="VH752_000756"
     CDS             19763..20089
                     /locus_tag="VH752_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ8289121.1"
                     /translation="MPFKTNLKAGVLLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVFFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            20187..21140
                     /locus_tag="VH752_000757"
     CDS             20187..21140
                     /locus_tag="VH752_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ8289122.1"
                     /translation="MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLAPVNLLTSPQSQLLKQWLKDYAQVVALITLPDSIFGHPSNAKSIIV
                     LQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN"
     gene            21199..22395
                     /locus_tag="VH752_000758"
     CDS             21199..22395
                     /locus_tag="VH752_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEQ8289123.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEEVLAQGIIERIGLKDSISTV
                     KYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVV
                     VNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYH
                     GKSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSG
                     VSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGE
                     NAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGDISTPESKVKVLVISTDEELCIARDVE
                     RLKNTK"
     gene            22582..22890
                     /locus_tag="VH752_000759"
     CDS             22582..22890
                     /locus_tag="VH752_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289124.1"
                     /translation="MYVFIEPNDLKDYLITVYDERNRAIRKQTQVYCFYLMIVLFVAL
                     FFFFPCLTIRDLVSYKLALIFAYLYLIIRSLVAKKMEALGKITTNSQKPPFDTSQVLC
                     "
     gene            complement(22973..23743)
                     /locus_tag="VH752_000760"
     CDS             complement(22973..23743)
                     /locus_tag="VH752_000760"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEQ8289125.1"
                     /translation="MKIGIIGVGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLT
                     LPYAMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRDLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGMPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTTTVSSAIDKTIDKAKSL"
     gene            complement(23791..24858)
                     /gene="pepA"
                     /locus_tag="VH752_000761"
     CDS             complement(23791..24858)
                     /gene="pepA"
                     /locus_tag="VH752_000761"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEQ8289126.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPHIEDIVFDGGFTDKAEAESFGITPGDI
                     IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEDAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKRY"
     gene            25314..25604
                     /locus_tag="VH752_000762"
     CDS             25314..25604
                     /locus_tag="VH752_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEQ8289127.1"
                     /translation="MRDMSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRKRRQMTRDL
                     RTFFSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDK"
     gene            25604..25921
                     /locus_tag="VH752_000763"
     CDS             25604..25921
                     /locus_tag="VH752_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEQ8289128.1"
                     /translation="MIRPTSYESLAALVEKEDKLVLFFTADWCPDCQFIYPIMPEIEA
                     ELTDMTFVCVNRDQFIEVAQKWDIFGIPSFVVIEKGQEVGRLVNKMRKTKTEIMHFLA
                     AYQ"
     gene            25939..26565
                     /locus_tag="VH752_000764"
     CDS             25939..26565
                     /locus_tag="VH752_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEQ8289129.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEASSLITIEGNGQIFLTDEDLARLNAELAKEGFSERLEPTVGPVFVVGQIV
                     EMVAHLDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMPNGSLIFPGK
                     LRGEESYGMMCSPRELALPNAPQKRGIIELDESAVVGEAFDPAKHWKG"
     gene            26717..27112
                     /locus_tag="VH752_000765"
     CDS             26717..27112
                     /locus_tag="VH752_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ8289130.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISVVVWGKLAETLVSYASKGSLISIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(27372..28013)
                     /locus_tag="VH752_000766"
     CDS             complement(27372..28013)
                     /locus_tag="VH752_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEQ8289131.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIEKRGRSFEQVDGNPSLEQYY
                     HQVHGEYPTWYEDYKVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(28033..29010)
                     /gene="dusB"
                     /locus_tag="VH752_000767"
     CDS             complement(28033..29010)
                     /gene="dusB"
                     /locus_tag="VH752_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEQ8289132.1"
                     /translation="MIKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFETVR"
     gene            complement(28997..29869)
                     /gene="hslO"
                     /locus_tag="VH752_000768"
     CDS             complement(28997..29869)
                     /gene="hslO"
                     /locus_tag="VH752_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEQ8289133.1"
                     /translation="MDKIIKSIAQSGSFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSRITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYTSTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEVARYEKRLQEMPAISHLLASKNHVDALLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNESDLEAIINDKA"
     gene            complement(30016..31509)
                     /gene="rofA"
                     /locus_tag="VH752_000769"
     CDS             complement(30016..31509)
                     /gene="rofA"
                     /locus_tag="VH752_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HEQ8289134.1"
                     /translation="MIEKYLESSIESKCQLVVLFFKTSSLPITEVAEKTGLTFLQLNH
                     YCEELNAFFPDSLSMTIQKRMISCQFTHPSKETYLYQLYASSNVLQLLAFLIKNGSHS
                     RPLTDFARSHFLSNSSAYRMREALIPLLRNFELKLSKNKIVGEEYRIRYLIALLYSKF
                     GIKVYDLTQQDKNIIHSFLSHSSTHLKTSPWLSESFSFYDILLALSWKRHQFSVTIPQ
                     TRIFQQLKKLFVYDSLKKSSRDIIETYCQLNFSAGDLDYLYLIYITANNSFASLQWTP
                     EHIRQCCQLFEENDTFRLLLNPIITLLPNLKEQKASLVKALMFFSKSFLFNLQHFIPE
                     TNLFVSPYYKGNQKLYTSLKLIVEEWMAKLPGKRYLNHKHFHLFCHYVEQILRNIQPP
                     LVVVFVASNFINAHLLTDSFPRYFSDKSIDFHSYYLLQDNVYQIPDLKPDLVITHSQL
                     IPFVHHELTKGIAVAEISFDESILSIQELMYQVKEEKFQADLTKQLT"
     gene            31741..>32714
                     /locus_tag="VH752_000770"
     CDS             31741..>32714
                     /locus_tag="VH752_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester-forming surface-anchored protein"
                     /protein_id="HEQ8289135.1"
                     /translation="MVSSYMFARGEKMNNKMFLNKEAGFLVHTKRKRRFAVTLVGVFF
                     LLLACAGAIGFGQVAYAADEKTVPNFKSPDPDYPWYGYDSYRGIFARYHNLKVNLKGS
                     KEYQAYCFNLTKYFPRPTYSTTNNFYKKIDGSGSAFKSYAANPRVLDENLDKLEKNIL
                     NVIYNGYKSNANGFMNGIEDLNAILVTQNAIWYYSDSAPLNDVNKMWEREVRNGEISE
                     SQVTLMREALKKLIDPNLEATAANKIPSGYRLNIFKSENEDYQNLLSAEYVPDDPPKP
                     GDTSEHNPKTPELDGTPIPEDPKRPDESSEPALPPLMPELDGEEVPEVP"
BASE COUNT         9841 a         5878 c         7086 g         9909 t
ORIGIN      
        1 aaaatttaat attttacgga aagatagcga agaggctaaa cgcggcggac tgtaaatccg
       61 ctccttcggg ttcgggggtt cgaatccctc tctttccatc ctaaacgggc atagtttaaa
      121 ggtagaacta aggtctccaa aaccttcagt gtgggttcga ttcctactgc ccgtgttaga
      181 aatatggcgg gtgtggtgaa gtggttaaca catcagattg tggctctgac attcgtgggt
      241 tcgattccca tcactcgcct attttattgg ggtatagcca agcggtaagg caagggactt
      301 tgactccctc atgcgttggt tcgaatccag ctaccccagt ttaaatttta tatttaaagc
      361 cggcgtggcg gaattggcag acgcgctgga ctcaaaatcc agtgtccgca aggacgtgcc
      421 ggttcgaccc cggccgccgg tatacttgaa aaacaaggtt tacaaacctt gttttttgtt
      481 ttgatcttaa gctgtagcta cgtagtacaa tctcttatat tactgataag gtctatttaa
      541 tgcgcaagat ttgtggtatg atgaatatga ttgagtattg atgctttatg ggattaccgg
      601 taaggttttg aaaaaggttt gtcactacta ggtgcttttt tagctataga aagacagtta
      661 tggattgatg tattgtaact aatgttggag gttaatggtg gttgagtttt gagatattta
      721 cactgataat cgggtggaaa ctggttgggt atcgggtaat ggagtgtggt tcttcgttta
      781 agcaaggaga ttatcgtttg gtggttcata tttgcttgtt aaatgttagg gatgagatgc
      841 tgagtcagca aggccatgaa gataaagctg actggccaga gccttgagat gtgacgtttg
      901 gtggaagtcc tttttggtga gatggcacag catatagtaa tattagatcc tgaggaagaa
      961 ttgggacttt cattaggctt gactggggta tgacctcact ttacaattca tttcgatgat
     1021 gggtttgatg atacgttttt agttcaggac tttgtagagt tggagaattt ggttctttat
     1081 gaggaagaag tgcaggtaat tcgttgagac agtcgtgaag atattttagg tatgattgat
     1141 gaaggttcgt tcattcctta tctgaagtat aaaataaatt tgtgctttga gatggttggc
     1201 caatataaag tccatcaatc ttagaagtaa taatttttga attttttctg aggtttgtgt
     1261 atatttgata gtgtactgat gtaagaagta aggagtacta tgatggcaat caatgagaga
     1321 gcttctgtaa aaatcaagtt aggccttgat actaagaata agcgatgaga tggttaccac
     1381 gatttatcga tggtcatgat gagtattgat ctgtgtgact atgtgacggt tgaccatata
     1441 gatgacaata agattgtctt tgcctcaaac tgtccgaaaa tacctattaa ctaagataat
     1501 gatgtttata agatagttca gctaatgaaa catcgatttc aagttaaaag aggagtttcc
     1561 gtttacttgg aaaagcggat tcccatgtgt gctggtatgg gaggaggttc tagcgatgct
     1621 gttactatca gagcattaaa tcagttatgg ctccttactc ttagccgaaa agatatgatg
     1681 gatattggta taccgattgg tagtgatgtg ccttattgct tgctttcagg ttgtgcccaa
     1741 gtcactggta aaggtgaagt ggtttgtcgc attttggggc tgttatcgtc ttgggtggtt
     1801 ttggttaaac cagattttgg tatctcgact tgaacatttt ttctagatat taattgtaaa
     1861 atcatttcaa gagtgtctac tactcatttg gttgcggcga ttgaagcggg taactataat
     1921 gatgggatat tgactgaaat gaacaatttg ctggaagata ttttcattgc caagcgaccc
     1981 tttattcaaa aaataaaaga gaaaacactg caagctggtg ctgctactac tttgatgaca
     2041 ggaagtggac cgactgtttt tgctttgtgc cagaccgaaa aacaaactat aatagtttaa
     2101 aaggactttg caaggaagtt tttaaggtga gaatgttgta atattttgtt gggattgctt
     2161 ctttggaagc ttcatgttat aattaactgg ttaatgaaag aggagattta tggggacttt
     2221 agaaaaaaaa cttgataact tagtaaatac tattttatta aaagcagaaa atcagcatga
     2281 gttattattt ggagcttgtc aaagtgacgt taagcttact aatacgcaag aacatatttt
     2341 aatgttacta tctcagcaac gtctcactaa tacagatttg gctaaggcat taaatattag
     2401 tcaggcggca gtaactaagg ctatcaagag tttggtcaaa caagacatgc tagcaggaac
     2461 taaggatacg gttgatgcta gggtgactta ttttgaatta accgagttag ctaagcctat
     2521 tgcgtcagaa catacccatc atcatgatga aaccttaaat gtttacaacc gtttattaca
     2581 aaaattctcc gcgaaagaat tagagattgt agataagttt gtaacagttt ttgctgagga
     2641 attagaaggg taatccatga gatacatatc agtgaaaaat ctctcctttc aatatgaaag
     2701 tgagccagtt ttagaaggga tcacttatca tttagatagt ggagaatttg tcaccatgac
     2761 cggtgaaaat ggcgctgcaa agtcaacctt aataaaagca accttaggaa ttttacaacc
     2821 aaaggctgga cgagttacta ttgctaaaaa aaataaagac ggtaaacaat taaggattgc
     2881 ttacttgccg cagcaagtag ctagctttaa cgctggtttt ccatccaccg tttacgagtt
     2941 tgtcaaatca ggtcgctacc cacgtagtgg ttggtttaga catttgaaca aacacgatga
     3001 agagcatgtg caagcaagct tagaagcagt cggcatgtgg gaaaaccgtc ataagagaat
     3061 tggtagttta tcaggtggtc aaaaacaacg tgtggttatt gcccgtatgt ttgcttctga
     3121 ccctgatatt tttgtgctag acgagccaac aacgggaatg gatagcggta ctactgatac
     3181 cttttatgaa ctgatgcacc acagtgcaca tcaacacggg aaatccgttc tgatgattac
     3241 ccatgaccca gaagaagtga aggcttatgc tgatcggaac attcatttag tcagaaacca
     3301 aaaacttcct tggcgttgtt tcaacattca tgaagttgaa acagatgacg aaaaaggagg
     3361 tcatggtcat gcttgatatt ttatcctatg atttcatgca acgggcggta atggcggtag
     3421 ttgccattag tatttttgct ccgattttag gtattttcct tattttacgt cgtcaaagtt
     3481 tgatgagcga tacccttagt catgtttctt tggctggggt agcgcttggg gtagtccttg
     3541 gtatttcacc aaccatcact actattattg ttgtggtttt agctgctatt ttgttagaat
     3601 acctgcgtgt agtttacaaa cactacatgg agatttcaac ggcgattttg atgtcacttg
     3661 gcttggccct atctctgatt attatgagta agtcgcatag ttcatcaagc atgagtttag
     3721 aacaatacct ttttggatcg atcatcacga ttagtatgga acaagttgtc gccttgtttg
     3781 ctattgctgc gattatttta atcttgaccg ttctcttcat tagaccgatg tacattctga
     3841 cctttgatga agatactgct tttgtagatg gtttgcccgt tcgcttgatg tctgttctat
     3901 tcaatatcgt cactggggtt gctattgctt tgaccattcc agcagcagga gcacttttgg
     3961 tttctaccat tatggtcttg ccagcaagta tcgcaatgag attgggtaaa aactttaaaa
     4021 cagttatctt actgggaatt gtcatcggtt ttagcggtat gttatctggt attttcttat
     4081 cttatttctt tgaaacgcca gctagtgcca ctattaccat gattttcatt agtattttcc
     4141 tcttagttag tctaggtgga atgcttaaaa aacggttatt ttaataagaa aaatcctcac
     4201 atgtttaaca tgtgaggatt tatttataaa tgctttaaaa agggggtaag gtcccaattt
     4261 tcttgattaa tggtgatgct ctcccaaaag ctatcattag ttaaacgagg aatcaaatga
     4321 aggtgaaaat gagtcccctc atccatcaac tccttgtcat tgctgacgct agtgacgcct
     4381 gaaatgggaa ggtgctgctc tagcttagca actagagcgg cttgtaagtc gatgagatca
     4441 tagcgcactt cctttggtag ttgtgtcaga ctagtgtaat ggtctttgga aatcagtaaa
     4501 aggtgcccct tttggatagg atcaatatca aaaacaacct tgaaatattg actttgagtc
     4561 agtagctgat ttgctttgag ctgatggcaa aagatacatt ctgtcatagg tacatctcct
     4621 tatgaaaaaa cgagccagag ctcgtttaat cggttatcta gtaagtgaga acagcgtatt
     4681 ttttcttacc gcggcgaata acggttaatt gatcatcaat tttatcatca ttacttaatt
     4741 ggtaatctaa atcttgaacg cggtctccgt taatgtagat tgctccattt tgcacgtctt
     4801 cacgcgcttg gcgttttgat ggggaaatac ccgcggtgac aagcatgtca acaaggttaa
     4861 ggctgtcttc tgattgaacg tgataatttg gtacattgct caatccttgt tttaattcat
     4921 tagcagaaag atttttgatg tttccagcaa ataactgctc ggtaatgtta agagcttgtt
     4981 tataagcttc ttcgccatga accaaggtaa ccacttcgcg tgccaaggtt ttttgagcga
     5041 gacgctcgtg acgagcggca ttaaattgag tttcaatttc tgcaatctca tctagagata
     5101 agaaggtgaa gattttcaag aaacgcactg catcatcatc catgacattt aaccagaatt
     5161 ggtacatctc gtaaggagac gtcttatcgg catcaagcca gacagcatta ccttctgatt
     5221 taccgaattt tttcccggtt gagtcagtga tgagtggtac agtcatgacg tgcccagttt
     5281 tatcagcctt tttgcggagc aattcagtac cagctgtcat attaccccac tggtcagagc
     5341 cgccaatttg taaggttaca ttatgcttgt cattgagttc gtagaagtcg tagccctgca
     5401 tgatttggta agcaaactca gtgtaagaaa tgcctgtttc aatgcgtttt ttaacagagt
     5461 ctttactcat catgtagtta acggtgaagt atttaccgac atcacggagg aagtcaataa
     5521 agctgatttg cgagaaccag tcgtagttgt tgacaagctc tgctttatta tcaccatttt
     5581 caaaatcaag gaaagtagac aactgccctt taatcttgtc actccactcc aaaactgttt
     5641 ctttggtttg aaggctgcgc tctgcatctt taaaggaagg atcaccaatt aaacctgtgg
     5701 caccacccac aagagcgtaa ggtttgtgcc ctgctagttg caagcgacga gatgttaaga
     5761 tagccactag atggcccaga tgaaggctgt cagcggttgg atcataaccg gtataatagg
     5821 atacttgccc ttctgttaat gctttgacaa gggcttgttc atcagtcgtt tgaaagacca
     5881 agccacgagc tttgagttct tcaaaaatat tcataggctt tctccatttt cattatagtt
     5941 tcgaatcatt atatcaaaaa acgctactgc tatgcaagct ttatcaaagt ttggtataat
     6001 gataaatgag gtattttatg gtgaaatgga acacgaaaca aaagcgtata agtcatcaaa
     6061 gattaggtct cttggatttg gggccggtgc tattacgtac gttgagacta ctgtctaact
     6121 ttttttatat tgttatcttc ctttttggaa tgatgggatt tggtatggca tttgggtatt
     6181 tggctagtca gattgaatct gttaaggtac caagtaaaga aagtttagtc aaacaagttg
     6241 aatcattaac gatgatttcg caaatgaact attctgataa tagtttaatt tctactttag
     6301 atacggattt acttcgaaca ccagtagcta atgatgcgat ttcagagaat atcaaaaaag
     6361 ctattgtatc aacagaagac gaacattttc aagaacataa aggtgtcgtg ccaaaagctg
     6421 tttttcgggc aacattggct tctgtattgg gatttggaga agctagtgga ggttcgacct
     6481 taacacagca attagtcaag caacaagttt tgggagacga tcccacattt aagcgcaagt
     6541 ctaaggagat cgtttacgct cttgccttag agcgttatat gtccaaagac aatatcttat
     6601 gtgattatct taatgtttca ccttttggcc gtaacaacaa gggtcaaaat attgctggtg
     6661 ttgaagaagc tgcgcgtggc atttttggcg tttctgccaa agatttaacg gtgccacagg
     6721 cagcattttt ggcgggtctt ccgcagagtc ctattgttta ctctccttat ttgtcaacgg
     6781 gacaactgaa gtcagaaaag gacatggctt atggcatcaa gcgtcagcaa aatgttctct
     6841 ataacatgta ccgtacaggt gttctgtcta aaaaagaata cgaggactat aaggcttatc
     6901 cgattcaaaa ggattttatt caaccgggaa gtgcaatagt aaataatcac gattaccttt
     6961 attacacggt gttagcggat gctaaaaaag ccatgtatag ctatttgatt aagcgagata
     7021 aggtgtctag tcgtgacttg aaaaatgacg agactaaggc tgcttatgaa gagagagcct
     7081 taacagaatt gcaacagggt ggctatacca tcaccacaac cattaataag cctatttaca
     7141 atgcgatgca gacagcggca gctcagtttg gtggcctgtt agatgatggc actggtacag
     7201 ttcaaatggg aaatgtcttg acagacaatg cgactggtgc tgtgttaggt tttgttggtg
     7261 gtagagatta tgctctgaat caaaataatc atgctttcaa tacagttaga tcgccaggtt
     7321 ctagcattaa accgataatc gcttatggtc ctgctattga tcaaggttta atggggagtg
     7381 ctagcgtttt gtctaattac ccaacaactt actcgagtgg ccaaaaaatc atgcatgctg
     7441 atagtgaagg aacagccatg atgccacttc aagaggccct aaatacttct tggaacatcc
     7501 cagctttttg gacacagaaa ttactgcgtg aaaaaggggt cgatgtcgaa aattatatga
     7561 caaaaatggg ttataagatt gcagactact cgattgaaag tttacctcta gggggcggta
     7621 ttgaagtctc ggttgctcaa caaaccaatg cttaccaaat gctttcaaac aatggcttat
     7681 atcaaaagca atatattgta gataagatta ctgctagcga tggtacagtc gtttacaaac
     7741 atgaaaataa gccaattcgt attttttctg cagcaacagc tacgatttta caagaattgt
     7801 tgagaggtcc gattacttca ggcgctacga ctactttcaa gaaccgtttg gcggctatta
     7861 atccgtggct tgctaatgct gattggattg gtaagaccgg aacaactgag aattatacgg
     7921 atgtttggct agtcctgtct actccaaaag ttaccttagg cggttgggca ggacatgatg
     7981 acaatacctc attagcgcca ttaacaggat ataacaataa ttctaattac cttgcctatt
     8041 tagctaatgc cattaatcag gccgatccca atgttattgg agtagggcaa cgtttcaact
     8101 tagatccagg agtcattaag gcgaatgtct tgaagtcaac aggtttacaa ccaggaactg
     8161 ttaatgtcaa tggacatact ttttctgttg gtggagaaat gaccaccagt ctatggtccc
     8221 aaaaaggacc gggggctatg acttaccgat ttgctattgg tggcacggat gccgattatc
     8281 aaaaagcttg ggggaacttt gggttcagaa aaaattaggt tgccatttat cgataaaaag
     8341 ggtataatag caataactat ttgtcgtcca atctagctga aatattgtcc agttaggaag
     8401 aacagcagtt aaatcacact gataaagtca gatttagctg ctctttttgt gtctattttt
     8461 agaaaagata gggtttgtaa cctatattta aatattctaa aaattaacat ttagggcaaa
     8521 tgataactta gttgcgattt gctgaaacag caaagcttaa atagaagaag gagctaaaaa
     8581 cttggcagga catgaagttc gatacggaaa acaccgtaca cgtcgtagct tttcaagaat
     8641 caaagaagtt cttgatttac caaatttgat tgaaattcaa actgactcat tccaagattt
     8701 cctcgattca ggtttgaaag aagtatttga agatgtactt cctatttcaa actttacgga
     8761 tactatggaa cttgaatttg ttggttacga atttaaagaa cctaaatata cccttgaaga
     8821 agctcgtatc cacgatgcaa gttattctgc accaatcttt gttaccttcc gtttggtcaa
     8881 taaagaaact ggtgagatta aaactcaaga agtcttcttt ggtgatttcc caatcatgac
     8941 ggaaatgggt acctttatta tcaatggtgg tgaacgtatc atcgtttctc agttggtgcg
     9001 ttctccaggt gtttatttca acgataaagt tgataaaaac ggtaaagttg gttacggatc
     9061 aacagtgatt cctaaccgtg gggcttggtt ggaattagag actgactcaa aagacattgc
     9121 ctacactcgt attgaccgta cccgtaagat tccattcaca accttggttc gtgcccttgg
     9181 tttctcaggt gatgatgaaa ttgttgatat ctttggtgaa agcgaccttg ttcgtaatac
     9241 cattgaaaaa gacattcaca aaaatccaag tgattctcgt acagacgaag cccttaaaga
     9301 aatttacgag cgtcttcgtc caggtgagcc aaaaactgca gatagctctc gtagtctttt
     9361 gattgcgcgt ttctttgatg cacgtcgtta cgatttggct gcggttggtc gttacaaagt
     9421 gaacaaaaaa cttaatatca agactcgtct tttgaaccaa atcattgctg aaaaccttgt
     9481 ggatgctgaa actggcgaaa ttttggtaga agctggaact gagatgactc gtagcgtcat
     9541 cgaatccatc gaagaacacc ttgacggtga tttgaacaag tttgtttaca caccaaatga
     9601 ttacgctgtg gttactgaac ctgttgttct tcaaaagttc aaggttgtgt caccaattga
     9661 tcctgatcgt gtagtaacta ttgttggtaa tgccaatcca gatgacaagg ttcgtgcatt
     9721 gacacctgct gatattttgg cagaaatgtc ttacttcttg aaccttgcag aaggtcttgg
     9781 aaaagttgac gatattgacc acttaggtaa ccgtcgtatc cgtgccgttg gtgaattgct
     9841 tgccaaccaa ttccgcatcg gtcttgctcg tatggagcgt aacgttcgtg agcgtatgtc
     9901 tgttcaagac aacgatgtgt taacaccaca acaaatcatc aatatccgtc ctgtcacagc
     9961 agctgtcaaa gaattcttcg gttcgtctca gttgtcacaa ttcatggacc aacacaaccc
    10021 attgtcagag ttgtctcaca aacgtcgttt atctgcctta ggacctggtg gtttgacacg
    10081 tgaccgtgct ggttatgagg ttcgtgacgt gcattacacg cactatggcc gtatgtgtcc
    10141 gattgaaaca cctgaaggac caaacattgg tttgattaac aacttgtctt catttggaca
    10201 tcttaataaa tatggtttca tccaaacacc ttaccgtaag gttgaccgtg cgactggtag
    10261 ggtaactaac gagattgttt ggttgactgc cgacgaagaa gacgaataca cagttgcaca
    10321 ggccaattcg aaactaaacg aagatggcac ttttgctgaa gaaatcgtta tgggtcgtca
    10381 ccaaggtaat aaccaagaat tttctgcaag tgttgttgat ttcgttgacg tttcccctaa
    10441 acaggtagtt gctgttgcga cggcatgtat tcctttcttg gaaaacgatg actccaaccg
    10501 tgccctcatg ggtgccaaca tgcaacgtca ggctgtgcca ttgattgatc caaaagcacc
    10561 atatgttggt actggtatgg aatatcaagc tgcccatgac tcaggcgctg cggtgattgc
    10621 tcagcacaat ggtaaagttg tcttttctga tgctgaaaaa gtggaaatcc gtcgtcaaga
    10681 tggctcactt gatgtttacc acattaccaa attccgtcgt tcaaactcag gaacagccta
    10741 taaccaacgc acccttgtta aagtaggaga cattgttgaa aaaggtgatt tcatcgctga
    10801 tggaccttct atggaaaatg gtgaaatggc tcttggacaa aacccagtcg ttgcttacat
    10861 gacttgggaa ggttataact ttgaggatgc cgttatcatg agtgagcgcc ttgtgaaaga
    10921 agatgtctac acatctgttc acttggaaga attcgaatct gaaacgcgtg atacaaaact
    10981 tggccctgaa gaaatcactc gggaaatccc aaatgttggt gaagaagccc tcaaagacct
    11041 tgacgaaatg ggcattatcc gtatcggtgc tgaggtgaaa gaaggcgaca tcttagtagg
    11101 taaagtcaca cctaaaggtg aaaaagacct ttctgctgaa gaacgtttac ttcacgccat
    11161 ctttggggat aaatcgcgtg aagtgcgtga tacctcactt cgtgtccctc atggtggtga
    11221 tggtattgtt cgtgatgtga aaatctttac acgcgctaac ggcgatgaat tgcaatcagg
    11281 tgttaatatg cttgtgcgtg tttacatcgc tcaaaaacgt aaaatcaagg tcggagataa
    11341 aatggccggt cgtcacggaa acaagggtgt cgtttcacgt attgtaccag ttgaagacat
    11401 gccatacctt ccagacggaa caccagttga catcatgttg aaccctctcg gggtgccatc
    11461 acggatgaat attggtcagg ttatggaact tcaccttggt atggctgctc gtaatcttgg
    11521 cattcacatt gcaacacctg tctttgacgg ggcttcatca gaagaccttt gggacactgt
    11581 tcgtgaagct ggcatggata gcgatgctaa gacggtcctt tatgatggtc gcaccggtga
    11641 accatttgac aaccgtgtat ccgttggtgt catgtatatg atcaaacttc accacatggt
    11701 tgatgataaa cttcatgccc gttctgtagg accatactca cttgttaccc aacagccact
    11761 tggtggtaaa gctcaatttg gtggacaacg ttttggtgag atggaggttt gggcccttga
    11821 agcttatggt gcatcaaatg ttcttcaaga aatcttgacc tacaagtcag atgacgtcac
    11881 aggacgtttg aaagcctatg aagcgattac taaaggtaaa ccaattccaa aaccaggtgt
    11941 accagaatcc ttccgtgttc ttgtaaaaga attgcaatcg cttggtcttg atatgcgtgt
    12001 gcttgacgag gatgataatg aagtggaact tcgtgatctt gatgaaggtg aagacgatga
    12061 tattatgcat gttgacgatc tcgagaaggc acgtgaaaaa caagctcaag aaacccaaga
    12121 agtttctgaa acaactgacg aaaaataagc aatcaattct tattaaataa ttatttactg
    12181 gtctggggca aaggccccag gaactggtaa agtcatcaaa ggcagaaagg taaaactagt
    12241 ggttgacgta aatcgtttta aaagtatgca aatcacatta gcctcaccaa gtaaggtccg
    12301 ttcatggtct tatggtgaag ttaaaaaacc tgaaacaatc aactaccgta cattaaaacc
    12361 agaacgtgaa ggactctttg acgaagtcat ctttggtcca acaaaagact gggaatgtgc
    12421 gtgtggtaag tacaaacgta tccgttataa agggatcgtt tgtgaccgct gtggggttga
    12481 ggtaactcgt gctaaagtac gtcgtgaacg tatgggtcac attgagttaa aagctcctgt
    12541 atcacatatc tggtatttca aagggattcc atctcgtatg ggattgactc ttgatatgag
    12601 tcctcgtgcc cttgaagaag tgatctattt tgcggcttat gtggtcattg atccaaaaga
    12661 tacaccgctt gaaccaaaat cattattaac agaacgtgaa taccgtgaaa aactacaaga
    12721 gtatggtcat gggtcatttg tcgctaagat gggtgctgaa gccattcaag atctcttgaa
    12781 acgtgtggac ttggcagctg aaatcgctga attaaaagaa gagttgaaat ctgcttctgg
    12841 tcaaaaacgg attaaagcag ttcgtcgttt agacgtcctt gatgccttta acaaatctgg
    12901 aaataaacca gaatggatgg ttcttaacat cctgccggtt attccaccag atcttcgtcc
    12961 gatggttcaa ttggatggtg gtcgttttgc ggcatcagac ttgaatgact tgtaccgtcg
    13021 tgtgattaac cgtaacaacc gtttggcacg tttgttagaa cttaatgccc ctggcatcat
    13081 tgttcaaaat gaaaaacgaa tgcttcaaga agccgttgat gctttgattg ataatggtcg
    13141 tcgtggtcgt ccaatcactg gaccaggtag tcgtccattg aaatcattga gccacatgct
    13201 taaaggtaaa caagggcgtt tccgtcaaaa cttgcttggt aaacgtgtag acttctctgg
    13261 acgttccgtt atcgctgttg gtccgaccct taaaatgtat caatgtggtg tcccacgtga
    13321 aatggctatc gagcttttta aaccatttgt aatgcgcgaa attgttgcca aagaatatgc
    13381 tggtaacgtt aaagccgcta aacgtatggt tgaacgtggc gacgaacgca tctgggatat
    13441 cttagaagaa gttatcaaag aacacccagt cctacttaac cgcgcaccga ctcttcacag
    13501 acttggtatt caggcttttg aaccggttct tattgacggt aaggcacttc gtcttcaccc
    13561 acttgtgtgt gaggcctaca atgccgactt cgatggagac caaatggcca tccacgtgcc
    13621 actttcagaa gaagcacaag ctgaagctcg tcttttgatg cttgctgcag agcacatctt
    13681 gaaccctaaa gatggtaaac cagttgttac cccatctcag gatatggttc ttggtaacta
    13741 ctatcttacg atggaagatg ctggtcgcga aggcgaaggt atgattttca aggataaaga
    13801 cgaagctgtg atggcatatc gtaatggcta tgctcatctt catagtcgtg tgggtattgc
    13861 tgttgacagc atgccaaaca aaccttggaa agacagtcaa agacataaaa ttatggtgac
    13921 aactgttggt aagattttgt ttaacgacat catgccagag gaccttcctt acctccaaga
    13981 gccaaacaat gccaacttga cagaaggaac acctgataaa tacttccttg aacctggtca
    14041 agacatccaa gaagtgattg acggcttaga catcaatgtg ccatttaaga agaaaaacct
    14101 cggtaacatc attgcggaaa ccttcaaacg tttccgtaca acagaaacat cagccttcct
    14161 tgaccgcttg aaagaccttg gttactacca ttcaaccctt gctggtttga cagtgggtat
    14221 cgctgacatt cctgttattg ataataaagc tgaaatcatt gatgctgctc accatcgtgt
    14281 tgaagaaatt aacaaagcct tccgtcgtgg tttgatgaca gatgatgacc gttatgttgc
    14341 cgttacaaca acatggcgtg aagctaaaga agcccttgaa aaacgtctga ttgaaacaca
    14401 agatcctaag aacccaatcg ttatgatgat ggactcaggt gcccgtggta acatctctaa
    14461 cttctcacag cttgccggta tgcgtggttt gatggctgct cctaacggac gcatcatgga
    14521 acttcctatc ttgtcaaact tccgtgaagg tttgagcgtt ttggaaatgt tcttctcaac
    14581 ccacggtgca cgtaaaggga tgaccgatac ggcccttaaa acagccgact caggttatct
    14641 tactcgtcgt ttggttgacg ttgcccaaga tgttatcatt cgtgaggacg attgtggcac
    14701 tgatcgtggt cttcttatcc gtgctattac agatggtaaa gaagttaccg aaacgcttga
    14761 agagcgtctt caaggtcgtt acacacgtaa atcagtcaaa caccctgaaa ctggtgaagt
    14821 cttgattggt gctgaccaat taatcactga agacatggct cgtaagattg ttgatgcagg
    14881 tgttgaagaa gtgaccattc gttctgtctt tacctgtgcg actcgtcatg gtgtctgccg
    14941 tcactgttat ggtatcaact tggcaactgg tgatgctgtt gaagtgggtg aagcagttgg
    15001 tactattgct gcccaatcta tcggtgagcc tggtactcag cttaccatgc gtaccttcca
    15061 cacgggtggt gtagcctcaa ataccgatat cacccagggt cttcctcgta ttcaagagat
    15121 ctttgaagca cgtaatccta aaggggaagc ggtcattact gaagtgaaag ggaatgtcgt
    15181 cgagattgaa gaagatgcgt caactcgtac caagaaagtc tacgttcaag gtaaaactgg
    15241 catgggcgaa tatgtcgtac catttacagc acgtatgaaa gttgaagttg gcgacgaagt
    15301 taatcgcgga gctgccctta cagaagggtc aattcaaccg aaacgtctcc ttgaagtgcg
    15361 tgataccttg tcagttgaaa cgtaccttct tgcagaagta caaaaagttt accgtagcca
    15421 aggggtagaa atcggagaca aacacgttga ggtaatggtt cgccaaatgc ttcgtaaagt
    15481 tcgtgtcatg gatccaggtg atacagacct ccttccaggt acacttatgg atatttctga
    15541 tttcacagat gctaacaaag atattgttat ctctggtggt atccctgcga catctcgtcc
    15601 tgttcttatg ggtattacta aggcttccct tgaaaccaat tccttcttat cagctgcatc
    15661 cttccaagaa acaactcgtg ttcttacaga tgctgctatc cgtggtaaga aagaccactt
    15721 acttggtctt aaagaaaatg ttatcattgg taaaatcatc ccagcaggta ctggtatggc
    15781 gcgctaccgt aacattgaac cacaagcgat gaatgagatt gaagtgattg atcatacaga
    15841 agtctcagca gaagcagaat aatctcatga aagagtcctt aggggctctt tcttgtctga
    15901 gtttgttggc agtaaaaagc attgacaatc agtcttagtc ccaatgagca gatgtttttc
    15961 aaaatactta tgatttagct ataataatgc ataagaggaa tgggtaatgt gttatgtatc
    16021 aagtaattaa aatgtatggt gattgggagc cttggtggtt catcgatgga tggcaagacg
    16081 atattataga tgagcaacaa tttagtgact ggcaagaagc gcttgattat tttaatcaag
    16141 aatggcagcg catgaaagct atttttccta gttatcatag ccaaaagaat ttgttagcta
    16201 ctttttggga aaaagaagat aaaagatggt gcgaggactg tgacgaagat ttgcagcagt
    16261 ttcattctct tttactcctt aaaaataaag atattgtacc aagcaataat tatattcctg
    16321 aatttgaaca acgaaatgat tcaccacagg tagcttatct ttgcaagtta aacctttaag
    16381 tgtttgttaa aaacaaaata agttaaaaga aaatatcatt tcttatgata ttttcttttt
    16441 ttgtttacga ataaatgagt aaggaggaat tatggtacaa gcattagcaa aagctattct
    16501 agcaaaagct gaacaggttc aggcacaaga tatttatatt ttgccaagag cagatcaata
    16561 tgatcttttt ttacgaatag gagatgaaag gagattagtt gatgtttatc agagcgatcg
    16621 gatggctcct cttattagtc actttaaatt cgttgcagga atgatagttg gtgaaaagag
    16681 acgttgtcag gtgggttcat gtgattataa gcttagtaaa gataagcagt tatctttgcg
    16741 cttatctagc gtgggtgatt atcgcgggca agaaagctta gtgattcgtc tgcttcatca
    16801 tcaaaataaa agtgtacatt attggtttga tggattgaca aaagtagcca atcaagttgg
    16861 cggtagaggg ttgtatttat ttgcaggacc agttgggtct gggaagacaa ccttgatgta
    16921 ccagctgatt tcgagttatc atcaagaagc acaggttatt agtatagaag atcctgtaga
    16981 aattaaaaat caccaaattt tacaattaca agtgaatgat gatattggta tgacttatga
    17041 caatttgatc aaactgtctt tacgccatcg accagatatt ttagttattg gtgagattcg
    17101 agatagtcaa acagcaagag ccgttattag ggctagtcta acaggtgcca tggttttttc
    17161 aacggttcac gctaaaagta tctcgggtgt ttatgcaaga ttgttagaac ttggtataac
    17221 gaaagcagaa ctgtctaatt gcttagcatt aattgcttac caaaggttac ttaatggagg
    17281 agcattgatt gactctactc aaaacgaatt tgaatattat tcctcatcga actggaatca
    17341 acaaattgat cagcttcttg aggcaggaca tctcaatccc aagcaagcta agcttgaaaa
    17401 aattatctag taaacatcag cataaattca ttcaattact agctaatctt ttatctacag
    17461 gatttagctt cgcagaggtt attgcttttt taaaaagaag ccagttgctt cagctagatt
    17521 atgttcttaa aatggaagag tctttattaa aaggacaagg cttagcagat atgttgtcgg
    17581 gtttaggttt ttcagatgct attcttactc aaattagttt agctgataga cacggtaata
    17641 ttgaaacaac attagtagcg attcaacatt acttgaacca aatggcaagg atcagacgaa
    17701 aaactgttga agttatcacc tatcctctta ttcttttact ttttcttttt gtgatgatgc
    17761 tagggttgag acgctattta gtccctcaat tagaaactca aaatcagata acttactttc
    17821 ttaaccattt tcctgccttc tttataggtt tttgctcagg tcttattttg ttgttcggga
    17881 tggtatggct acgatggcga tctcaaagcc gcttgaaact ctatagtcgg ctaagtcgtt
    17941 atcctttttt aggtaaactg ttaaaacaat acttaactag ttattatgct agagaatgga
    18001 gtacgttaat aggtcaaggt cttgatttga tgactatttt agacattatg gccattgaga
    18061 aatcctcact aatgaaagaa ttagctgagg acatcagaat gagtctgctt gaagggcaag
    18121 cctttcacat taaagtagct acctaccctt tttttaaaaa agaattaagt ttaatgattg
    18181 aatacggtga aatcaaatcg aaactggggg ttgagttgga aatctatgcc caggaaagct
    18241 gggaacaatt ttttagtcag ctttatcaag tgacacaact tattcaacca gctatttttt
    18301 tagtagttgc tgtgacaatc gtcatgattt atgcggcaat cctattacca atttaccaaa
    18361 atatgggagg tattttttaa gatgattaat caatggaaca acttacgaca caagaagtta
    18421 aaaggattta ctcttctaga aatgttattg gtgattcttg tcatcagtgt tttgatgcta
    18481 ttatttgtgc ctaatttaag caagcaaaaa gacagggtta cagaaacagg taatgccgct
    18541 gttgttaaat tagtggagaa tcaagcagaa ctatatgaat tatctcaagg ctcaaaacca
    18601 agtttgagcc agttaaaggc agatggtagt atcactgaga aacaagaaaa agcttatcaa
    18661 gactattatg acaaacataa aaatgaaaaa gcccgtctta gcaattaaag cttttacttt
    18721 gcttgaaact ttactaagtt tgtcagtgat gagtttcatt atattgggat tgtctgtacc
    18781 agtgacaaaa agctatcaaa aagtagaaga gcatctgttt tttagccatt ttgaacattt
    18841 gtatcgacat cagcaaaagt tagccatatt acagcaaaaa cagcgcgtct tggatatttc
    18901 ttcaacaaaa attgttacag aaggtaatag cctaacagtt cctaaatcaa taacagtgaa
    18961 tcatccttat cggttagtta ttgatcagat gggaggcaac cattccttag ctaaaattat
    19021 ttttgacatg actgatcgac gctttaaata ccaattttat ctagggagtg ggaattatca
    19081 aaaaacaagt caaagcttac atagcccttg aaagtatgat tgcaacaggc atcctcttta
    19141 gtattgtcat tctcgtctta agcagtttac agcagagtca ggctgctcta acgtactatc
    19201 gaaagcagca agaaaagctt aatctagcct tgatggcagt acaaactaga actaaagaga
    19261 tgacattgaa tggttgtcat attactattt tacgtacgga tagatacatt agtattcacg
    19321 atgacgaagg agaagtgatg aagattgagt aaacaattaa gtaacataaa agcttttacc
    19381 ctcctagagg cgttaatagc cttactcgtg atatcagggt ctttattggt ttatcaaggt
    19441 ttgacccaaa ccctccttaa acgtagccat tacctagccc gtcatgatca agacaattgg
    19501 ctcttatttt ctcatcaatt gcgagaggag ttaagtggag caagattttg caaagtagct
    19561 gataataaac tatacgttga aaaaggaaag aaagtactag cttttggcca atttaaaagt
    19621 catgattttc gaaaatcagc tagtaatgga aaaggatatc aacccatgtt atttggaata
    19681 tcacgtagtc atattcacat agagcagtca cagatttgca ttactttaaa gtggaaaagt
    19741 gggttagaaa ggacttttta ttatgccttt caagactaat ctcaaagcag gcgtcctttt
    19801 gtatgctatg tttatggcta gtattttttt gctggtttta caggtttacc tttctcaagt
    19861 gacagctcta cacaaggaat accaagctca aactgactat gttaaagccc gactgatagc
    19921 agaaatagtt tatcaagatc atagatataa agcttctaat cctgtctttt ttaaaggtgg
    19981 tcaagtcatt tgtcgagagc gaaaagagag atggatgtta attgttaagt tagatcagca
    20041 acgacagtat cagtttgaat accttaaacc caaacaatat agtcaataaa tagtattgtt
    20101 gtcataaatc cttaaaatga tccaatatat agtaattaaa ggtgacactt cttttcattt
    20161 caagtgtaat ttggtatgat aaggttatga cttttgaaaa aattgaagaa gcttatcagc
    20221 tgctcttaga gaactgtcaa ctaattgaaa atgatctaaa aacacatata tacgatgcca
    20281 ttgttgaaca aaactccttt tatttagggg ctgagggagc tagtcctcaa gttgctcaaa
    20341 atagtgataa actgaaagcc ttgtgcctga caaaagaaga atggcgtaag gcctaccagt
    20401 ttctttttat taaggcagct cagactgagc aactccaagc caaccatcag ttcacaccag
    20461 atgctattgg cttcattctg ctgtatcttt tggaacaatt gagtgataaa gatagcttag
    20521 aggtacttga gattggaagt ggaacaggga acctagccca aacccttctc aacaacacga
    20581 gcaagagcct tgattatgta gggattgaac ttgatgatct cttgattgat ctgtcagcca
    20641 gtattgctga gataatggat tcttcagctc attttattca agaagatgcg gtaaggcctc
    20701 aattactaaa agaaagtgac attgtcatca gtgacttacc agttggttat tatcctaacg
    20761 atgatattgc caaacggtac aaggtggcta gttcagataa gcatacctat gcccatcatt
    20821 tattaatgga acagtcttta aaatacttga aaaaagacgg ttttgcgatt tttctggcac
    20881 cagtcaattt attgacgagc cctcagagtc agttattgaa acagtggtta aaagattatg
    20941 ctcaggtggt ggccttgatt acgctaccag attctatttt tggtcatccc tcaaatgcca
    21001 agtccattat tgtcttacaa aaacaaacag accacccaat ggaaaccttt gtctatccaa
    21061 ttcgggattt gaagcttgca gagaatattc atgattttat ggaaaatttc aaaaaatgga
    21121 aactgagtaa tgtcaattaa ttgatttttt gttataataa aaaagcatca cagaaaacga
    21181 tttcaagaga ggttaaaaat gtcaaagaca attgcaatta atgcaggtag ttctagttta
    21241 aaatggcaac tttatcagat gccagaagaa gaagtgttag cacaaggaat tattgagcgt
    21301 atcggtctca aggattctat ttcaacagtt aagtacgatg gcaaaaaaga agagcaaatt
    21361 cttgatattc acgatcatac agaagctgtt aaaatcttat tgaacgattt gattcacttt
    21421 ggaatcattg cagcttatga tgaaattaca ggagttggac accgtgttgt tgctggtggt
    21481 gaacttttca aagaatccgt tgtggttaat gacaaagttc ttgaacagat tgaagaatta
    21541 tccgttttag ctccacttca taatcctgga gcggcggcag ggattcgtgc cttccgtgat
    21601 attttgccag atattaccag tgtttgtgtg tttgacacgt catttcacac cagcatggca
    21661 aaacatactt acctttaccc aattcctcaa aaatattata cggactacaa ggttcgtaaa
    21721 tatggagctc atgggacaag tcataaatat gtggcgcaag aagctgctaa aatgcttggt
    21781 cgtccattag aagaattaaa actaattact gctcatattg gaaatggtgt ctcaattaca
    21841 gctaattacc acggtaaatc agttgatacc tcgatgggct ttacaccact tgctggtcct
    21901 atgatgggaa cacgttctgg tgacattgac ccagctatca ttccttattt aattgagcaa
    21961 gacccagagt taaaagacgc tgctgacgtt gttaatatgt taaacaaaaa atcaggtctt
    22021 agtggggtgt caggcatttc tagtgatatg cgtgatatcg aggctggttt acaagaagat
    22081 aatccagatg ctgttttagc ttacaatatc tttattgatc gcattaaaaa atgcattggt
    22141 caatattttg ctgttttgaa tggagcagat gctttggtct ttacagctgg tatgggtgaa
    22201 aatgcaccat tgatgcgtca agatgttatt ggtggcttaa catggttcgg aatggacatt
    22261 gatcctgaga aaaatgtttt tggataccgt ggtgatattt caacacctga gtcaaaagtg
    22321 aaggttcttg ttatctcaac agacgaagaa ttgtgcatcg cgcgtgacgt tgagcgtttg
    22381 aaaaatacaa aataaaagag actaagtctc aatagattaa ccaagtaagg caacacttta
    22441 cttggttttt tatgaggttt atctgttagt tatggtgcct aggtattagt tctgattagt
    22501 ctccttgctt tttgtaatct tgtgctaggc gatttggatg tcagccttat ttagaagttt
    22561 ttaattgctt caacgctttt cttgtatgtg tttatagagc ccaatgatct aaaagactat
    22621 ttgataacgg tttatgatga gcgtaatcgt gcgattcgaa aacagacaca agtttactgc
    22681 ttttacctga tgattgttct atttgttgcc ttattctttt tcttcccttg cttaaccatt
    22741 cgagaccttg tttcttataa gctagctttg atttttgctt acctttactt gattatcaga
    22801 agtctggtgg ctaagaaaat ggaggcacta gggaaaataa caactaatag tcaaaaacct
    22861 ccgtttgata cgagtcaggt tctttgttag atgacttgtc taacggttga gtttcctgtc
    22921 tggtcaacgg aggtgtttgt attgttgtct gatttgtatt ggggcactta ttttacaagc
    22981 ttttagcctt atcgatggtt ttgtcaatgg cagagctgac agtagttgtg aggccaaggc
    23041 gttctaactc catcagacca gcaatagttg tgccaccagg gctacaaata gcgtcaatga
    23101 aatcgtgcgg actttgagaa ctggtcttga gattgctggc actagccaag actgtttgag
    23161 taacaatctc cagcgccttt gctttaggca tgccattctt gacgccagcc ttagccaaag
    23221 cctcaataaa gagataaata taggcaggac ttgaccctgc taaagcggta aaggtgtcaa
    23281 aatccttttc actaatatca aatgtgctac caaagctatc tgttaagtct cgaacacgtg
    23341 cttgtaattc ctgagacacc aaagcatttc ccgttaaagc ggtactgctt tggagaattt
    23401 gtgcattcat gtttggcatg atacgtagca gcggaaggtc ttgtcctacg aatgttgcta
    23461 gtcgttgaag ggaaatgcct gctgccatag atataatagg ctgtttgaag tgaagcggtt
    23521 tgagtaccgt ttcaaatagt tgaggcttga tgcctaaaat aacaagatca acctggtcaa
    23581 taaggtcttg gtgggacata gcataaggca gtgttaactg ctccgcaatt tccttggacc
    23641 gttctaaaga tgatcctgaa atgataagtt catggggtgt ttgtttaagg cctttgatga
    23701 tagcgctagc cattttgcca acaccaataa tgccaatttt cattctgttg ctccttgtgg
    23761 gatattatgg ttaatcaggc taatgctagt ttagtaacgt ttaatcaagt caacggttga
    23821 gcgatccagt tttttgataa tggcttgtaa gaaggcttgg gcttctacga aatcatccat
    23881 agcgtagagg gtttgatgag agtgaatgta gcgtgcacag actccgatgg ttgttgatgg
    23941 gacaccacca ttttgaaggt gtgcagcacc tgcatctgtg cctcccttgc cacaatagta
    24001 ttggaaattg acaccagcgt cctcagcagt agtcagtaag aagtcgcgca tatctttgag
    24061 catgacatgg cctgggtcgt agaaacgcaa caaggtgcca tccccgattg ttccaggatt
    24121 gccataaata tcaccagcag gcgaacaatc tactgcgaaa aagagttcag ggtcgaactt
    24181 ggtggttgag acgtgggctc cgcgcagacc aacttcttct tgaacgttag cacctgcaat
    24241 tagggtgttg ttaaggtctt gtccttttaa cgcttcaagc atttctgtta tcatgagaac
    24301 gccatagcga ttgtcccaag cttttgaaat aatatttttt tgattggctg ttaggatcgt
    24361 ttcagattga gggacaataa tatcacccgg tgtaatgcca aagctttcag cttctgcctt
    24421 atctgtaaag ccaccatcaa acacaatatc ttcgatatgt ggtagactag cagagccatt
    24481 tgccccacgg agaaaatgag gaggtaccga tcctgaaata aggggaataa cttggccagt
    24541 gcgtgtgtaa agggtaaacc gttgtgaact gacaacaagt gggttccaac caccgatacc
    24601 aaccacgcgt agcgttccgt caactttgat atcactgacc ataaaaccga cttcgtccat
    24661 gtgcgcagct actaaaatac ggggggcttt ttcagcttta ctatctctga taccaaaaat
    24721 gccaccaagc ccgtctgttt caacacggtc aaccagcggg gttattttgg tgcgtaggta
    24781 gtcacgaacg ctatgttcat agcccgcaat gccatccagt tcggtaactt ctttgatttt
    24841 tgagaataag tctgtcattt ttcatctcca tttaaaaggt ttcagaccag ttcaacgcgg
    24901 tcatctcctt atattctacc actttttaag taagaatggg tttcacagaa aagaaaaatc
    24961 ccagagacta gagatctctg gaagaagtgt ttgtcttttg gcgataatct gtttttttag
    25021 gcagcaagga aatcattaaa atgataaagc acaagctggt taataagaat agtagggcgc
    25081 ttaggatgcg aaaagactct tcactagcga aaagtaagga taatggagtc ataaggagaa
    25141 ccttccattt taggttaatc agtgattttt atagcgtggt taaattaata ataaaccaca
    25201 cctaatcaac gtacctttag tttagttgtt tttactattt ttgtcaatac ataacagtag
    25261 aaatctttag ttttcttatt tcagatcgtc caatttgatc tttttatgat aaaatgaggg
    25321 atatgagtaa aaagaaaata ggtatgattt caggtatctt tggatttagt ttagctattg
    25381 gcctaggaat agttatcaaa gattattgcc aagataggaa gcgccgacaa atgacaaggg
    25441 atttacgcac ctttttttca cctttaggac aaatcgaagt tttatatatc aatccttgtc
    25501 aggtcaaaca agattatatt tcaggtgggg ttgttatgtc aaacggcaaa cagtaccaat
    25561 tcacctatca cagtcgacaa attagttttg aggaggacaa gtgatgatca gaccgacttc
    25621 ttatgagtca ttagcggctc ttgttgaaaa agaagataag ctggtgttat tttttacggc
    25681 agactggtgt ccagactgtc aatttattta tcctattatg ccagaaattg aagcagaact
    25741 tacagatatg acttttgttt gtgttaatcg tgatcagttt atagaggtag cacaaaagtg
    25801 ggatattttt ggtattccta gttttgtcgt gattgaaaaa ggtcaggaag tagggcgtct
    25861 ggttaacaaa atgagaaaga caaaaactga gattatgcat tttttagctg cttatcaata
    25921 agaaaagagg aagaataaat gatttttgca tacaataaag aacaagttgg cgatgtcttg
    25981 atggttatct tacaagacac caaagatatc aaacgtcaag tagaacgaaa aggcaaagta
    26041 gcccgtgttt ttgcagaaga aagcggcaaa acccttgctt ggaatatctt tgaagcgtca
    26101 agcttgatta ctattgaagg caatggacag atttttttga cagacgagga ccttgcaaga
    26161 ttaaatgcag agcttgctaa ggaaggattt tcagaaaggc ttgaaccgac tgtgggacct
    26221 gtttttgtgg tcggtcaaat tgttgagatg gtggctcatc tagatagcga ccatcttaat
    26281 atctgtcaag tggctattgg tgaagatcaa acggttcaaa tcgtagcggg cgcaccaaat
    26341 gctgcgcttg gtttaaaaac gattgttgcc ttaccaggtg ctataatgcc aaatggtagt
    26401 ctgattttcc caggaaaatt gcgtggtgaa gaaagttatg gtatgatgtg ctctcctcgt
    26461 gagttagcct tgccaaatgc accgcaaaaa cgtggcatta ttgaacttga tgagtcagct
    26521 gtggtaggag aagcttttga cccagccaaa cattggaaag gttagaaaaa ccagctgaaa
    26581 taagactgta tttaactaaa tgaccagaaa aaaaggatta ggatccccta ttcctttttt
    26641 cttatgtttt caaaaacgtt taaaatgaaa cttattttac cacaattttt acgaataata
    26701 agataaggag gtcagtatgt ataataaagt gatagcaatc ggtcgtttgg tagctaaacc
    26761 agaattggta aaaacagcta cggataagca tgtagcacgt ctctctttag ctgttaatcg
    26821 aagatttaaa aatgcttctg gagagcgaga agctgatttt atttcagttg ttgtttgggg
    26881 aaagttagca gaaactctgg tttcttatgc tagcaaaggt agtttgatat ctattgatgg
    26941 cgaacttagg acccgcaagt atgataaaga tgggcaagtg cattatgtga cagaagttct
    27001 ctgccaatca tttcaactgc ttgaaagtcg tgctcagcgc gctatgagag aaaataatgt
    27061 tactaatgat ctagttgatt tagtcttaga agaagatact cttccctttt gatagaggat
    27121 aatgcttctc atcagggttc agctgattac tgaaatatat gatgaggtga tatccgagca
    27181 acaaaagtgt gactagatac ttctgataaa aataaaaaaa caccttacga gtatgaacaa
    27241 agtttcaagt gctagatgtt ggaactttgt tcatcatagg aatagtaggg tgttttttag
    27301 tgtattactt ctttttgttg ctggtgttaa ggtttacttg cttacaagcg gttatcaggc
    27361 cactagaact cttataataa atggagctct ttgagtttgg tgtcaatcat ttttaagaca
    27421 gtcgccaaat cttgtgggtt ttgaacgaaa tcaagggaat tgccatcaat tttcattttt
    27481 ggagagacct tgtagtcttc gtaccaggtt gggtattcac cgtggacttg atggtaatat
    27541 tgctcaagac taggattgcc gtcgacctgc tcaaaacttc ggccacgttt ttcaatgcgc
    27601 tcaagcattt tgtcaaaaga cacatcaatg tagatgagaa gatcagggcg ttttttaggc
    27661 atgccttcta gttcttctaa catgttggcg agaagttcct gatagatgtc aagttctgtc
    27721 ttagtgacat tgccattttt gtagttaagt tttaagaaga gttcgtcttc aaaaatagag
    27781 cggtcaagaa tgttgttatc ggcttgataa gcttctttga tggacttaaa ccgtttattc
    27841 aaaaaataaa tttgaagtaa aaaggcgtac tttttagggt cttggtagta gaggtctagc
    27901 acggggttat tatcaacagc ttcgtaaaaa acgtcagttc ctaaatgctc tccaagtgca
    27961 gcagcaagcg agctcttgcc agccccaatt gttccagcta gtacaatcaa catgcgtctc
    28021 cttctaatgt cattagcgta ctgtttcgaa gatggcttca acctcagcta gagtctcagc
    28081 gcgtgaaaca gcaccacgaa ctttggcagc acctgctgtt ccacgaagat agtgtggcgc
    28141 taagccccga aattcccgaa cagcaatggt ttcacctttg aggttaatca ggcgtttaag
    28201 gtggtctttg gcgatgtcta attttttggc aaagggaaga tctggcaatt cttgaccagt
    28261 ttcaaagaaa tgattgattt gggtaaaaag ataaggattg ttcatggctg cgcggccaat
    28321 catcacggca tctacaccga tttcttcaat cataaatttg gcgtcttgca ccgaacgcac
    28381 gtccccattt ccaataaagg gaatcttagt gatagcctta gaaactcggg ctaatgtctc
    28441 atggtcgcag gttcctgtat acatttgttc acgggtacga ccgtgcatgg caagagcaga
    28501 tacacctgcc gactcagcgg caagggcatt ctcaacggca agtgaactat ccgcccaacc
    28561 tgtacgcatt ttgacagtca gtggaatatc taaaacagac gtgacttcct tgacaatgtg
    28621 gtaaatctta tcagggtcac ggagccactt ggcaccagcc tcgtttttga caactttatt
    28681 aacagggcag cccatattaa tatcaacaat atcggccttg gtattggttt gaatgaaatc
    28741 agctgcacgt ttcaaccctt ctgcatcacc accaaataat tgaatggaca tagggtgctc
    28801 attctcgtca atatggagca tatggagggt cttttcgttg ttgtagagca gccctttttc
    28861 ggaaatcatt tccataacca ccaaaccagc accaaattct ttggcgatgg tacgaaaggc
    28921 agagttggtc acaccagcca tgggagcaag gacagtgcgg tgagggattt ccacatcacc
    28981 aatcctaaag gaagaattaa gctttatcat tgataatggc ctccaaatca ctttcattaa
    29041 attggtactt ggtaccacaa aattggcaaa caatctcagc acccttatct tcgtcaatca
    29101 tggcctgtaa atctgctttt ggcaaagtca ttagggctgc ttcaaaacgt tctctagaac
    29161 agtcacactg aaaactcaga ggttcttcag aaagacgctt gtaaggttca tctccataga
    29221 tggcctccaa aagggcatcc acatgatttt tggaagctag taggtgagaa atggcaggca
    29281 tttcttgcag gcgtttttca tagcgagcaa cctcttcttc agaagctcct ggtagcacct
    29341 gcaccataaa gccaccagca accttgacct tatcattttc atctaaaagc acattaagtc
    29401 cgattgcaga tggtgtttgc tctgattcgg ttaagtagta ggcaaaatcc tcaccaattt
    29461 ccccagtaat aagaggcgtt gttgaggtgt aagggttacc tgtgccataa tcaataatcg
    29521 tcacaaaatg accattcccc ataaaaggcc caacaaggac ttcacctgtt gcggtctttt
    29581 taatatcgac accagtattt tgaatgtaac ctttcacatg tcctttggta tctgccacag
    29641 agataatgtg tccaaaagaa gaatcaccaa tcactttgac ggtgattcta ctatcgcctt
    29701 tttgattagc cgccaaaatc tggttggcga tcaaggtacg gccaagcgca acagtagacg
    29761 atgacagcgt gttgtgtttt tcttgagcta atgcaactgt ctcagtgcta tcaaggacat
    29821 aagccctaaa agaccctgat tgtgctatcg atttaattat tttatccata acttagatta
    29881 tagcataaag tgtaaaactt gtattgacag gattgcttga aaaatcaata cagatagaca
    29941 gtaaaaaatc ccagagaatg actcgggatt aagttaaggt gtggtctaaa gtcgcgcaat
    30001 gtggtgacat ggcgcttatg ttaattgctt ggttaaatca gcttggaatt tttcctcttt
    30061 aacttgatac atcaattctt ggatagacag aatcgattca tcaaaagata tttcagcaac
    30121 agcaattcct tttgtaagtt catggtgaac aaaaggaatc agttgactgt gagtgatgac
    30181 caaatctggc tttaaatcag gaatttgata aacattatct tgcaatagat aataggaatg
    30241 aaaatcaatg cttttatccg agaaatacct tgggaaagaa tctgttagga gatgagcatt
    30301 gataaaatta ctggctacga aaacaacaac taaaggaggt tggatatttc ttagaatttg
    30361 ctcgacatag tggcaaaaaa gatgaaaatg cttatggttc aagtaacgct taccaggaag
    30421 tttggccatc cactcttcga caattaactt taaggacgta tagagttttt ggtttccttt
    30481 atagtacgga gaaacgaata agttggtctc aggaataaaa tgttgcagat taaacaagaa
    30541 tgattttgaa aaaaacataa gagcttttac taaactagcc ttttgctctt ttaggttagg
    30601 taaaagagtg atgataggat ttaaaagcag gcgaaaagta tcattttctt caaaaagttg
    30661 acaacattgt ctgatatgct caggtgtcca ttgtaagctc gcaaaagaat tattagcggt
    30721 gatataaatt aaatagaggt agtccaaatc tcctgctgaa aagtttagtt ggcagtaagt
    30781 ttcgataata tcacggctac tttttttcaa agaatcgtag acaaaaagtt tttttaattg
    30841 ttgaaaaatt ctggtttggg gaatagttac cgaaaattga tgccgcttcc acgataaagc
    30901 taataaaatg tcatagaaag aaaacgattc cgataaccaa ggagaagttt taaggtgggt
    30961 ggaactatgg gataaaaagc tatgaataat gtttttgtct tgctgcgtca agtcataaac
    31021 tttaatgcca aacttactat atagcagagc gatgaggtaa cggatacgat attcctcacc
    31081 gacaatcttg ttcttagaga gttttaattc aaagtttctt aataaaggaa tcaatgcttc
    31141 gcgcatccga taagctgagg agtttgataa aaaatgactt cttgcaaaat ccgtaagggg
    31201 acgagagtgg gaaccatttt ttattaaaaa cgctagtaat tgtaagacat tagatgatgc
    31261 atagagttgg taaagataag tttctttaga aggatgtgta aattggcaag atatcatcct
    31321 tttttggatg gtcatagaca gactatcagg gaaaaaggca ttcagctcct cacaataatg
    31381 gtttagttgt aaaaaggtta agccagtttt ttctgctacc tcagttattg gcaaagaaga
    31441 tgtcttaaaa aacaagacaa ctaactgaca ttttgattcg attgatgatt ccaagtattt
    31501 ttctatcaag gaaaggggtc ctctctttct acacttagaa agccaaataa gtctttgata
    31561 agtgtattct cctatttcaa ttctatctaa gctaaaaata gaaatcaaat ctaatggttt
    31621 ttggtgaaaa gtcgaaacct tttgaaatag ctttaaagca atattttctc aaaaaatcat
    31681 tttcaaaaac attaatttgg tgaaatatac aatcaaaaaa atatctttct tgacaataac
    31741 atggtaagct catatatgtt tgcgagagga gagaaaatga ataacaaaat gtttttgaac
    31801 aaagaagccg gttttttggt acacacaaaa agaaaaaggc gatttgctgt cactttagtg
    31861 ggagtctttt ttctgctttt ggcatgtgcg ggtgctatcg gttttggtca agtagcctat
    31921 gctgcggatg agaagactgt gccgaatttt aaaagcccag atccagatta tccctggtat
    31981 ggttatgatt cgtatagagg aatatttgca agatatcaca atttaaaagt aaatctaaaa
    32041 ggaagtaagg agtatcaagc gtattgtttt aacctaacaa aatactttcc tcgccccact
    32101 tatagtacta caaataattt ttacaagaaa attgatggga gtggatcagc gttcaaatct
    32161 tatgcagcga atcctagggt tttagatgag aatttagata aattagaaaa aaatatactg
    32221 aatgtaattt ataatggata taaaagtaat gcaaatggtt ttatgaatgg tatagaagat
    32281 cttaatgcta tactagtaac tcaaaacgct atttggtact attcagatag tgctccatta
    32341 aatgatgtta ataaaatgtg ggaaagagag gttcggaatg gggagattag tgagtcacaa
    32401 gttactttaa tgcgtgaggc attgaaaaaa ctaattgatc ccaatttaga agctactgca
    32461 gctaataaaa tcccatcagg atatcgttta aatatcttta agtctgaaaa tgaagattac
    32521 caaaatcttt taagtgctga atatgtacct gatgatcccc ctaaacctgg tgatacgtca
    32581 gaacataatc ctaaaactcc cgagttggat ggcactccaa ttcccgagga cccaaaacgt
    32641 ccagatgaga gttcagaacc tgcgcttccc ccattaatgc cagagctaga tggtgaagaa
    32701 gtcccagaag ttcc
//
LOCUS       DAUGHY010000017        32356 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.017, whole genome shotgun sequence.
ACCESSION   DAUGHY010000017 DAUGHY010000000
VERSION     DAUGHY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32356
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.017"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            14..853
                     /locus_tag="VH752_000771"
     CDS             14..853
                     /locus_tag="VH752_000771"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEQ8289136.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMFAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFD
                     LAI"
     gene            933..1430
                     /locus_tag="VH752_000772"
     CDS             933..1430
                     /locus_tag="VH752_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEQ8289137.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAIL
                     MGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRIIKTVIHHRFKGDTYRPNLDFSHFTQESQTFYARDAKNPYDFTVT
                     ALKHK"
     gene            1750..2979
                     /gene="clpX"
                     /locus_tag="VH752_000773"
     CDS             1750..2979
                     /gene="clpX"
                     /locus_tag="VH752_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEQ8289138.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELSEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLKTSDLIRILTEPRNALVKQYQA
                     LLSYDGVELEFDKAALEAIATKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            2989..3588
                     /locus_tag="VH752_000774"
     CDS             2989..3588
                     /locus_tag="VH752_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEQ8289139.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPQDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDDKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTTRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESVVKKALNFDKSDTFIVFSSVERIGIDDSWDAILEQV"
     gene            3736..4479
                     /locus_tag="VH752_000775"
     CDS             3736..4479
                     /locus_tag="VH752_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289140.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKALKKSPSSV"
     gene            complement(4537..6636)
                     /locus_tag="VH752_000776"
     CDS             complement(4537..6636)
                     /locus_tag="VH752_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ8289141.1"
                     /translation="MTHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATNSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPHRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKESQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQSQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGNRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSGSNIMDRIAPYFRPEFLNRFNSIIKFNHLQKENLEEIVDLMLAEVN
                     QTTAKKGISLTIADDAKAHLIDLGYNHAMGARPLRRIIEQEIRDRITDYYLDHPEVKK
                     LQAILKEGQLVIRQKDQ"
     gene            7014..7697
                     /gene="rpiA"
                     /locus_tag="VH752_000777"
     CDS             7014..7697
                     /gene="rpiA"
                     /locus_tag="VH752_000777"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEQ8289142.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEGLGIPLKSIDDIDSIDLTVDGADEVDKDFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     IVLEAPKAG"
     gene            7774..8985
                     /locus_tag="VH752_000778"
     CDS             7774..8985
                     /locus_tag="VH752_000778"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEQ8289143.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     TAGLSVPNMAKIGLGNISRPIPLKTIPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIFANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSASFTGNGLIPQGHFADISATVAENFGVDTA
                     MIGESFLGHLK"
     gene            9004..9444
                     /gene="arsC"
                     /locus_tag="VH752_000779"
     CDS             9004..9444
                     /gene="arsC"
                     /locus_tag="VH752_000779"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEQ8289144.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAHGLANQAVAEKDVINAMLADPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYMKEDGESVNPIEVKGEKDVIDD"
     gene            9428..10237
                     /locus_tag="VH752_000780"
     CDS             9428..10237
                     /locus_tag="VH752_000780"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ8289145.1"
                     /translation="MSLMTKINETKDFLVTKGIETPEFGLILGSGLGELAEEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTK
                     VYRNKAHEMAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQHIKEDFKGLVKAILAEL"
     gene            10900..11613
                     /gene="deoD"
                     /locus_tag="VH752_000781"
     CDS             10900..11613
                     /gene="deoD"
                     /locus_tag="VH752_000781"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ8289146.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMALGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKVGLETLIAND"
     gene            11606..12394
                     /locus_tag="VH752_000782"
     CDS             11606..12394
                     /locus_tag="VH752_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEQ8289147.1"
                     /translation="MTNNQTLDILLDVYAYNHAFRIAKALPNIPKTALYLLEMLKERR
                     ELNLAFLAEHAAENRIIEDQYHCSLWLNQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWHFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADVLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYRCEPFYFDDMNAIIKKELSLWRQSIV"
     gene            complement(12474..13379)
                     /locus_tag="VH752_000783"
     CDS             complement(12474..13379)
                     /locus_tag="VH752_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ8289148.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLSMDDLSDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHSKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            13601..14137
                     /locus_tag="VH752_000784"
     CDS             13601..14137
                     /locus_tag="VH752_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289149.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     PAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDVLYEHIQRVNLEE
                     ETFIKYYESPKVSTGEFE"
     gene            14414..15106
                     /gene="pyrF"
                     /locus_tag="VH752_000785"
     CDS             14414..15106
                     /gene="pyrF"
                     /locus_tag="VH752_000785"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEQ8289150.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQG
                     PDIVRSIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGVVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            15164..15793
                     /locus_tag="VH752_000786"
     CDS             15164..15793
                     /locus_tag="VH752_000786"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEQ8289151.1"
                     /translation="MTLASQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFVYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKEDQVNWQQ"
     gene            15990..17444
                     /locus_tag="VH752_000787"
     CDS             15990..17444
                     /locus_tag="VH752_000787"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEQ8289152.1"
                     /translation="MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGLPFAGVPLFLKDLGQELKGHSSTSGSRLFKEYQATKTDLFVK
                     RLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPKDNTRNAGGSSGGAAALVSSGIS
                     ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKDSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALKQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LPMGIQLIAAKGREDLLLGIAEQFEAAGLLKIPQ"
     gene            17562..18425
                     /locus_tag="VH752_000788"
     CDS             17562..18425
                     /locus_tag="VH752_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ8289153.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHKSQKSSWDTIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     DVQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYYAGQDLTKPFGKDK
                     KIPVIDTKDVN"
     gene            18454..19104
                     /locus_tag="VH752_000789"
     CDS             18454..19104
                     /locus_tag="VH752_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ8289154.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFFTGLFLGLGLALLGR
                     SRQPLIHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWIIAFLLERYQEFLENKLALV"
     gene            19237..19890
                     /locus_tag="VH752_000790"
     CDS             19237..19890
                     /locus_tag="VH752_000790"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEQ8289155.1"
                     /translation="MAHSIWHEKIKSFLPEHYYGRINHFLDEAYASGLVYPPRENVFK
                     ALQVTPLEETKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWEPFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKAFITNPKHHIIESPHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGVDWLK"
     gene            20022..21290
                     /locus_tag="VH752_000791"
     CDS             20022..21290
                     /locus_tag="VH752_000791"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEQ8289156.1"
                     /translation="MILIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVQVVAFAQQLGAKVTAEVSPQHFSTTEDLLLTAGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFA
                     SKASNSPFIGNKLKGVVKYTIADGEVVYPN"
     gene            complement(21348..21989)
                     /gene="plsY"
                     /locus_tag="VH752_000792"
     CDS             complement(21348..21989)
                     /gene="plsY"
                     /locus_tag="VH752_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEQ8289157.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            22124..24073
                     /gene="parE"
                     /locus_tag="VH752_000793"
     CDS             22124..24073
                     /gene="parE"
                     /locus_tag="VH752_000793"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEQ8289158.1"
                     /translation="MTKKEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEALSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGSVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTISERLKESAFLLKNVKMSLTDLRGDD
                     PIIEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDQDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVESIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDSRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGADFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVETGHVYIALPPLYKMSKGKGKTEKVSYAWTDGEL
                     EDLRKEFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTIDDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            24164..26623
                     /gene="parC"
                     /locus_tag="VH752_000794"
     CDS             24164..26623
                     /gene="parC"
                     /locus_tag="VH752_000794"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEQ8289159.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQDWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEVRLSEIAGYLLQDIEKNTVSFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPTGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKDADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAEKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKADAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLEVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
     gene            26701..27768
                     /locus_tag="VH752_000795"
     CDS             26701..27768
                     /locus_tag="VH752_000795"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEQ8289160.1"
                     /translation="MKKSYNRKKQKRGTMMTIAIDWDNLGFEYHKLPFRYISYYKNGQ
                     WDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAARLQATAD
                     RLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYI
                     FTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAG
                     FSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLSPSILPSITKYSLLQLAEERLG
                     MTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
                     ELVGIQFGDIEAPEGWIVKVD"
     gene            27832..28062
                     /locus_tag="VH752_000796"
     CDS             27832..28062
                     /locus_tag="VH752_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEQ8289161.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETKIDGYQLQIGKRVVGEIAELDE
                     KFAVLKNDGVDCFFKTLEQAVENIIENYNLNH"
     gene            28123..28203
                     /locus_tag="VH752_000797"
     tRNA            28123..28203
                     /locus_tag="VH752_000797"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28157..28159,aa:Tyr,seq:gta)
     gene            28255..28326
                     /locus_tag="VH752_000798"
     tRNA            28255..28326
                     /locus_tag="VH752_000798"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28287..28289,aa:Gln,seq:ttg)
     gene            28453..29658
                     /gene="rpsA"
                     /locus_tag="VH752_000799"
     CDS             28453..29658
                     /gene="rpsA"
                     /locus_tag="VH752_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEQ8289162.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERISLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
     gene            29771..29842
                     /locus_tag="VH752_000800"
     tRNA            29771..29842
                     /locus_tag="VH752_000800"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29804..29806,aa:Arg,seq:ccg)
     gene            complement(30205..30507)
                     /locus_tag="VH752_000801"
     CDS             complement(30205..30507)
                     /locus_tag="VH752_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289163.1"
                     /translation="MTYELCLEYGTYPLSRVYAYWGEDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPEKRAQIRILYQEVATILKSKYKDYPIKIETFLL"
     gene            30707..31693
                     /locus_tag="VH752_000802"
     CDS             30707..31693
                     /locus_tag="VH752_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEQ8289164.1"
                     /translation="MSEKIRVLLYYKYVSIENAQEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWLVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENETKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPEFVGA"
     gene            31764..31955
                     /locus_tag="VH752_000803"
     CDS             31764..31955
                     /locus_tag="VH752_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289165.1"
                     /translation="MQQDTSERGHQAHFVMQSHQQRRRQHQLCLEPFQKNCEFLYYEI
                     EKVKLDSEDCLTINPLGMV"
     gene            32088..>32356
                     /locus_tag="VH752_000804"
     CDS             32088..>32356
                     /locus_tag="VH752_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEQ8289166.1"
                     /translation="MDFLTGDFPLVFRPMYNPHRYTISQDNQALEKVKQASYKRMDIA
                     MTHLDGLIGDSGHVYRDQQTIADAYAYAMALWSQKTPKSYENYPHL"
BASE COUNT        10137 a         5701 c         6747 g         9771 t
ORIGIN      
        1 aaggttatta gagatgacaa aagctgacca gatttttaaa gctaatattc aaaaaattat
       61 aaacgaagga tcattgagtg agcaagcacg tcctaaatat aaagatggtc gaacagctca
      121 ttctaagtat attactgggg cgtttgctga atatgattta gctaaaggag agtttcccat
      181 cacaacttta cgacccattc caattaaatc tgctataaag gaattatttt ggatatatca
      241 agatcagtct aacagtttag atgtgctaga agctaaatat aatgttcatt attggaatga
      301 atgggaagtt gaccagacac gtactattgg acaacgttat ggtgctgtag tcaaaaaaca
      361 tgatatcatc tcaaaaatac taaagcaatt agcagaaaat ccttggaatc gtcgtaatgt
      421 catttctctc tgggactatg aagcatttga ggagacaaaa gggttgttgc catgtgcctt
      481 tcaaatcatg tttgatgtta gacgtgtagg ggaagatctt tatttagatg ctagtttgac
      541 gcaacgttct aatgacatat tagttgctca tcacatcaat gccatgcaat acgttgcctt
      601 gcagatgatg tttgctaaac atttcggatg gaaaataggt aagttctttt attttgttaa
      661 caatttacat atttatgaca accaatttga tcaggctcaa gaattactaa aacgacagcc
      721 agttgccagt cagcctaagc ttgttttaaa tgttccagat ggtactaatt tctttgatat
      781 taaaccagat gattttgaat tacaaaatta tgatccagtg aagcctcagt tgcactttga
      841 ccttgctatt taaactttat ttagattttt tgtgagcatt ttgttcataa atttggtaaa
      901 ataaatagag tttaaaatga aggaaataat taatgacaaa ggaaattatt gctatttggg
      961 cagaggatga agcagggctt attggtgttg cgggtaagtt accatggtat ttaccaaaag
     1021 agttagaaca ttttaaaaag acaacgctac atcaagcaat cttaatgggc cgtgtgactt
     1081 ttgaaggaat gaattgcaaa agacttcctc aacgtcaaac ccttgtcatg acaagaaata
     1141 gagattatca agtagatgag gtgttgacaa tgactagtat tgaaaaagtc ttggagtggt
     1201 accatgcgca ggataaaaca ctttatatca ttggtgggaa taaggtttta gaagcgttta
     1261 atggttattt tgatcgtatc ataaaaacag ttatccatca tcgttttaaa ggagatacct
     1321 accgacccaa tctagatttt agtcatttca cgcaagaaag ccaaacgttc tatgctcgag
     1381 acgctaaaaa tccttatgat tttaccgtta ctgctttaaa acacaaatag tctgaggaga
     1441 tgataagcga tgcaacgtag tatttttgga gtatttacgg cttttttgtg cgtgatttgt
     1501 gttctttgtg ccatcccagc ttttaaaaag aatcgttatg gcttaggaag tctgttctta
     1561 ttaaatgcgt ttactaatct ggttaatagt attcacgcct tctatggtac attgttttga
     1621 gatgcgactt gcaaagacag cactggtgaa gatttagcta tcaagcaaga atcaccaaaa
     1681 accaatacaa cacattgact aatattaaaa ttcagtaaaa gataagagaa agtaagagaa
     1741 ttataagaaa tggcaggaag tagaactaac gatattaaag tatactgttc attttgtggt
     1801 aaaagccagg atgacgttaa aaaaattatc gcaggaaata atgttttcat ctgcaatgaa
     1861 tgcgtagctt tatcacaaga aattataaaa gaagaattgt ctgaagaagt ccttgccgat
     1921 ttgacagaag ttccaaaacc aaaagagctc cttgatgttt taaatcaata tgtggttggt
     1981 caagatcgtg ctaaacgtgc tttatcagtt gctgtgtaca atcattataa gcgtgtctcg
     2041 tttacagaaa gtcgtgatga cgatgatgtg gatttacaaa agtctaacat tttgatgatt
     2101 ggaccaacag gttcaggaaa aaccttctta gcacaaactc tggctaaaag cctcaatgta
     2161 ccatttgcta ttgcagatgc aacttctttg actgaagcag ggtatgttgg agaagatgtg
     2221 gaaaatattt tactcaaact gattcaggct gctgactata atgtcgaacg agcagagcgt
     2281 ggtattattt atgttgatga aatagataag attgctaaaa aaggtgagaa cgtatctatc
     2341 acacgtgatg tatctggtga aggtgtacag caagcccttc ttaaaattat tgaaggtacg
     2401 gtagcaagtg ttcccccaca aggtgggcgg aagcacccta atcaagaaat gatccaaatt
     2461 gatacaaaaa atatcttgtt tattgttggc ggggcttttg atggcatcga ggagattgtt
     2521 aaacaacgtt taggtgaaaa agttattggt tttggtcaaa acagccgtaa aattgatgac
     2581 aatgcttctt atatgcaaga aattatttca gaagatattc aaaagtttgg gcttattcca
     2641 gaatttattg gccgcttacc agttgtggca gcccttgaac aacttaaaac atcagatttg
     2701 attcgaatct taacagagcc aaggaatgct cttgtcaagc aatatcaggc ccttctttcc
     2761 tatgatgggg ttgaattaga atttgataaa gcagctcttg aagctatcgc aactaaagcg
     2821 attgaacgta agacaggagc acgtggtctt cgttctatta ttgaagaaac aatgcttgac
     2881 attatgtttg aaattccaag ccaagaagat gtcacaaaag tgcgtatcac caaggctgct
     2941 gtcgaaggca agtcaaaacc cgttttagag acagcttaag gagaaataat ggctgaagaa
     3001 caagtcttaa atacccataa cgcctcaatt ttattgagtg ctgccaataa atctcattac
     3061 ccccaagatg atttgccaga aattgccctc gctggacgtt caaatgttgg taaatcaagt
     3121 tttattaata ccatattggg gcgcaaaaat ttggcaagaa cctcaagcaa gccaggaaaa
     3181 acccaactat taaatttttt caatattgat gacaagctta gatttgtgga tgtccctggt
     3241 tatggctatg ccaaggtttc taaaagtgaa cgggccaaat ggggaaagat gatcgaagaa
     3301 tacttaacca ccagagataa tctgcgtgct gtcgtgagtt tggttgattt acgtcatgcc
     3361 ccttcaaaag aagatattca gatgtatgac tttttgaagt actatgacat tcctgttatt
     3421 gttgtggcaa ccaaagcaga taagattcct cgtggcaaat ggaacaaaca tgagtctgtg
     3481 gtcaagaaag ctttgaattt tgacaaatct gacactttta tagtattttc ttcggtagaa
     3541 cgtattggta tcgatgattc ttgggatgct attttagagc aggtctagcg ataggtgttg
     3601 atttatctat gctaaaacat tctcagtaga gggtgttttt cttatcgaaa acataaaaaa
     3661 tcatagttag gtttaccgat tttacttgac gccttaatga tgacatgcta gtattttata
     3721 ttaaggagat gagtcatgac acaagtagcc caagggcgct ctaaaggaaa gtcattgtta
     3781 tgccatcaat gcaatgccct ttttgtattt attatttttc ttatcttgct tgctgtactt
     3841 tttggtgttc gatatcgtaa tagctctatt gaaggtatct ggcgaacaac aagcattgat
     3901 cagaagttag gcgatgattt tgctaaacgt cttactggtt tgcatcagag ccctcttatt
     3961 gatgatagtt tattaacgtc atctcagatg atactaacag ttaaaaataa caacgttgac
     4021 ttgagtttta gtgttcaagt tgaacgagac atttttgtca aacgtttagc tgcttatcac
     4081 caaaatgaat tattaaagac attaaaggaa aatcatttag ttgttggtga cttgagtagt
     4141 aaagaacgcc aaattattga gaatagtatg ccagctagcc atgaattaga aatgatttta
     4201 gatcaagctt ttgagaaatt agcttctcag atcggaggca aatataatca aaaaacagga
     4261 catttatctg ctgtggttct caaaggaaaa gttaatcgta ttttgcacac cattgatatc
     4321 aaagaagaag tagcagcggg acatacttct ttttcaaaag gattgctcac acctaatggt
     4381 tattttgatt acaccagatt tggaaagaaa cttgagttat taggcgatga aaaaattatt
     4441 tttaaaaaag cgctaaaaaa aagtccgtca agtgtataac gtagtcacaa gacggatttt
     4501 ttcatgtttt gaaaattatt caattgaaga agtttattac tgatcttttt gcctaatcac
     4561 tagttgtcct tcttttagga ttgcctgaag ttttttgact tctggatggt caagataata
     4621 atcagttata cggtcacgaa tttcctgttc gataatacgt cgtaatggac gagctcccat
     4681 agcgtgattg taccctaaat ctatcaaatg agctttagcg tcatcagcaa tcgttaaact
     4741 aatacctttt tttgcggtgg tttgatttac ttcagctagc attaaatcaa caatttcttc
     4801 taaattttct ttttgcagat gattaaattt aatgatacta ttaaaacggt tcaaaaactc
     4861 tggtctgaaa taaggtgcaa tgcgatccat aatgttagac ccagaagttt ccgtatcttg
     4921 ttggccaaaa ccggcatttg atgtagcaat aataacggta tttttaaagt taatagtatt
     4981 tccttgtcca tctgtcaatc gtccatcatc taacacttgt agaagaagtg taatgatttg
     5041 aggatcagct ttttcaattt catctagtaa gacaatagca taagggttac gacgaacacg
     5101 ctcagttagg gtattattat tatcatcgta gccaacataa ccagccgttg tcccaatcaa
     5161 ttttgacaca gcagtgcgat cgttatattc ggacatatca agtcgaataa tagcatcttt
     5221 ggaaccaaat aaatcaagtg ctaattgttt agctaactct gttttaccaa caccagttgg
     5281 acccacaaaa aggaaagaac ctattggacg gttaccatca tcaaaacctg ctcgatttcg
     5341 tcgaatagca cgtgctactg ctgcgacagc ctcatcttgt ccgatgacat ggctttttaa
     5401 gcggctgcta attcctttga gcctttctaa atcattagtc cccatgttag agacaggaat
     5461 accagttaag cgctctacag actctgcgat atcattgact gtagcagtaa cattttggct
     5521 ttggttatgt tgctcaattt gcttttgtaa tgtttcaatc cgtgtttttg ctgctaaagc
     5581 tttttcaaag tcttctttgg cgacagcttt ttcttgactc tctttttgtt tagctatttc
     5641 agtttcgagt gtttttaagt cagtgaccgg gtgttgcgca gctaaatgag cagctgtcat
     5701 atccagtaag tcaatcgctt tatctggcag gctacggtgt gggatgtatt gaatagaata
     5761 atcaacagca gcttttagaa cattatcagg aagggtaata tggtggtgtt gttcataaag
     5821 attacgaata cccattaaaa tgtgaaaagt atcttcagct gatggtgcat tcactttaac
     5881 ttcattaaag cgacgagcaa gagcagcatt ttttaaaata gtattgcggt attcgtcttg
     5941 agtagtagca ccaatcagtg ttaattctcc tcgtgacaag gctggtttaa gaatatctgc
     6001 aagtcccttt gagcctgaat cactgcttgt ggcaccagca ccaactattt gatgaatttc
     6061 atcaaaaaat aagatgatat tcccagcttc ttttacttct tgaatcaagt tttgaatggt
     6121 ctcttcaaag gaaccacgat actgtgtacc agcttcaaga ctagaaatat caattgatac
     6181 gatttctttg tttttaatag cggctgggac atctccatta acaatggctt gagctaaacc
     6241 ttcaatgact gcagttttac cgacaccagc atctccgaca aggacaggat tatttttagt
     6301 tcgtcgtgcc aagatttcag cggtatcttg aatttctttg ttacgaccaa tgacggggtc
     6361 aagatgccct tgacgagctt cttgtgttaa gttagttccg agttgagtca atacactatc
     6421 agctttaggt gttaacattt gagaatttgt tgctttggtt gtcgctacag ggagttgtcc
     6481 tgtttgacga tagtgttgga attcttcggg agttatttcg cgaccgttaa cgagatagcg
     6541 gggattttcg ctacgataac cacccatgtt tgccattaat tggttgaaaa tatcatccat
     6601 atttacaaaa ggatctttac cagaaaaatg tgtcatatca gttacctcgc aataaaaaat
     6661 agactataag atgtttgtaa gcagtcgcag aatcgttgtt aggatttcac atgataggaa
     6721 accctttgct aataaaatga ttcagacatt atttttactc atcttatagt attattataa
     6781 tcaaaaggtc agtaaaggtc aaacaaaaac acttgatttt caaaaagcta aataaacacg
     6841 aaaagtaagt gcttatatga tgactttaga ataaatcata agttttaaaa agatcatcct
     6901 attctatagc acttaggctg tcaatgagag aaaaacaaat gttttcatta acaagttggc
     6961 gctttcatgg tataataaag tgctaagacg caattctatt ataaggggga atcatggaag
     7021 cactcaaaaa aattgccggt gttaccgctg cgcaatatgt gactgatggc atgaccattg
     7081 ggcttggaac tgggtcaacg gcttactatt ttgttgagga aattggccgt cgcgtaaagc
     7141 aagaaggact gcaagtggtt ggtgtgacta cgtcaagtgt gactagcaag caagcagaag
     7201 gcttaggcat tcctttaaaa tcgatagatg atattgatag cattgacctc accgtagatg
     7261 gtgctgatga agtggataag gatttcaatg gaatcaaggg tggcggcgca gccttactaa
     7321 tggaaaaaat tgttgcaaca ccaacaaaag aatacatctg ggttgttgat gcttctaaaa
     7381 tggtagagca tctgggtgcc tttaagttac cagtcgaagt cgttcaatat ggtgctgatc
     7441 gtttgttccg tgtctttgaa aaagctggtt acaaaccgtc ttttcgtatg aaaggtgata
     7501 gtcgtttggt gacagacatg caaaactata ttattgatct tgatttgggt tgtattaaag
     7561 atcctgttgc ttttggtcac ctgctagatg gaacagttgg tgttgtggaa cacggtctct
     7621 ttaatggtat ggttgataag gtgattgttg ctagtaaaga cggcgttatt gttttagaag
     7681 cgcctaaagc aggctagtct tagcatataa cagtgttaat atattttata ggtattgggg
     7741 agtcaaccgt aacctcccct agattggaga cttatgtcaa aatttaatcg tattcacttg
     7801 gttgttttag actctgtcgg tattggtgca gcgccagatg ctgacaaatt ctttaatgct
     7861 ggtgtagctg atacagattc tgacaccctc ggtcatattt cagaaacagc aggcttatcg
     7921 gttccaaata tggcaaaaat tggacttgga aacatttctc gtccgatacc actaaaaact
     7981 attccaacag aagacaatcc tactggttac gtgaccaaat tggaagaagt atctcttgga
     8041 aaagatacca tgacaggcca ttgggaaatt atgggcttga acattacgga accttttgac
     8101 actttctgga atggtttccc agaagagatt ttaactaaga ttgaagaatt ctcaggtcgc
     8161 aaaattattc gtgaagctaa taaaccctat tcaggaactg cggttattga tgattttggt
     8221 ccgcgtcaaa tggaaacagg tgagttgatt gtttacacct cagcagaccc agtgcttcaa
     8281 attgcagccc atgaagacat tattcctgtg gaagagcttt acaagatttg tgaatatgct
     8341 cgctccatca cgcttgaacg ccctgccctt ttaggccgta ttattgcgcg tccttatgta
     8401 ggcgaaccag gaaactttac acgtacggct aaccgtcatg actatgcagt gtctcctttc
     8461 caagataccg ttttgaacaa actggctgac gcaggcgttc caacttatgc cgttggtaag
     8521 attaatgaca tctttaatgg ttcaggtatt acaaacgaca tgggacacaa taagtcgaac
     8581 agccatggta ttgacacgct tattaagact cttcaattac ctgagtttac taagggattc
     8641 tcctttacta acctcgttga ttttgatgcc aactttggtc atcgtcgtga cccagagggt
     8701 tatcgtgact gcttgcatga atttgacaat cgcctacctg aaatcttcgc aaatatgaaa
     8761 gaagacgatt tgcttctcat cactgctgac catggtaatg acccaaccta tgcaggcacc
     8821 gaccacactc gtgaatacat tccattgctt gcctactctg catcctttac tggaaatggt
     8881 cttattccac aagggcattt tgcggatatt tcagcaactg ttgcagaaaa ttttggagtt
     8941 gatacagcca tgattgggga gtcattcctt ggtcacttaa aataagaaag ttagtaattg
     9001 ataatggaag aaatcacgat ttatcataat ccaaactgtg gcacttcacg taatgttctt
     9061 gccatgattc gccatgcagg tattgagcct actattattg agtatttgca gacgccacct
     9121 aatcgggaga ctttaattga gctcttacaa tcgatgggaa tctctgcgcg agaactcttg
     9181 cgaaccaatg ttcctgaatt tgaggctcat ggattagcaa atcaagcagt agctgagaaa
     9241 gacgttatta atgccatgtt ggcggatcca attttgatca atcgtcctat tgttgtgact
     9301 cgcaaaggag taaaactttg tcgtccttct gagaccttac tggatattct tcctgttccc
     9361 ctgcctagtc cttatatgaa agaggatggc gagagtgtga accctattga ggtaaaagga
     9421 gaaaaagatg tcattgatga ctaaaattaa cgaaacaaaa gactttttag ttactaaagg
     9481 aattgaaact cccgaatttg gattaattct gggttcaggc cttggtgaac ttgctgaaga
     9541 agtagaaaat gctattgtta ttgattatgc agatattcca aactggggca agtcaacggt
     9601 tgttggccat gctggaaagt tagtttacgg agatttagct ggccgtaaag tgctcgcctt
     9661 acaaggacgt ttccattttt acgaaggaaa tccgcttgaa gtggtgactt tccctgttcg
     9721 tgtcatgaaa gctcttggat gtgaaggcgt tcttgtcaca aatgcagctg gtggtatcgg
     9781 ctacggccca ggtaccttga tggcaatcac agaccatatc aatatgaccg gaaataaccc
     9841 actaattggt gaaaacttgg acgagtttgg tccacgcttc cctgatatgt ctgatgccta
     9901 tactaaggtt tatcgcaata aggcacatga aatggctgaa aaaatgaaca tcaaactaga
     9961 agatggtgtt tatatgggct tgactggtcc aacttatgaa acaccagcag aaatccgtgc
    10021 tttcaaagta ttaggagcag atgctgttgg catgtcaact gtaccagaag ttattgtggc
    10081 tgctcattct ggacttaaag tacttggtat ttcagctatt acaaactttg cagctggttt
    10141 tcaatcagaa ttgaaccatg aagaagttgt ggaagtgacc caacatatca aagaagattt
    10201 caaaggtctt gttaaagcta ttttagcgga gttgtaggac gaacaaaatc aactggagca
    10261 ttattacagg aatgttattt tatgtcgtca gtttggcaca tctgttcgca ataaaaaaat
    10321 caaaaaataa tcacggagtg tgagttccat gaatgttgaa ctcagattct agcttttcta
    10381 actaacataa ccaacgaagg gaaagagacg tattcggttt aaattgagta tggactcgaa
    10441 catttcttcg tattaatttt taaataataa aggaaagaga gttagtcctt tcttatttga
    10501 actcgatttt tagctctctt ctattcaata tcaattatga aaggaatggg gttgtattca
    10561 attgaatacg ggcggaaagc tcggaaacta gataaactaa cttttgagct cacacatggc
    10621 gttagttccc taaatttcag tcgctttctg tccgccctct gaccttctac aaaataaaag
    10681 gaaaggtagg gctaactgta ttcattgaat tgaatacggg cggagaactg tttttcttcc
    10741 ttataaaaaa taaaaaaata agaaaggtga gggctgagtt cattaatttg aattcgggct
    10801 aaggacttgg tcaaaaagat aagttgtcta gaaaatcagg attttctgcc aacttcctat
    10861 tttgccatcg ttcttttaac gccctcagta tcataattta tgtctatcca tatttctgcc
    10921 aaaaaaggcg atattgctga taaaattctt ctacctggag atcctttgcg cgcgaaattt
    10981 atcgctgaaa atttcctaga agatgcagtg tgttttaacg aagtgcgtaa catgtttggc
    11041 tacacaggaa cttacaaagg gcaccgcgtt tctgtcatgg gaactggtat ggggatgcct
    11101 tcgatctcta tctatgctcg tgaattaatc gttgattacg gtgtcaaaac attgatccgt
    11161 gttggtactg cgggtgcgat tgacccagaa gttcatgtgc gtgaattggt gcttgcccaa
    11221 gcagcagcaa ccaactcaaa tattatccgt aatgacttcc cagaatttga tttcccacaa
    11281 attgctgatt ttggtttact tgacaaggct taccatatcg ccagagaaat gggagtaaca
    11341 actcacgttg ggaatgtgct ttcttctgat gtcttttaca ctaacatgcc tgagcgtaat
    11401 atggctcttg gtaaattggg tgttaaagcc atcgaaatgg aagcagcagc tctttactat
    11461 ttagctgctc agcaccatgt caaagccctt ggaatcatga cgatctcaga taacttgaat
    11521 gatccaaccg aagatactac agctgaagag cgccaaacca ctttcactga tatgatgaag
    11581 gtcggcttgg aaaccttaat tgccaatgac taataatcaa acactagaca tccttttgga
    11641 tgtctatgct tataatcacg cctttagaat tgctaaagcc ttgccaaata tccctaaaac
    11701 tgccctctat ttactagaga tgttaaaaga gcgcagagaa ttgaaccttg cctttctggc
    11761 ggaacatgca gcagagaatc ggatcattga agaccagtac cactgttcat tatggcttaa
    11821 ccaatcgctt gaagatgagc agattgccaa ttacattttg gatttagaag ttaaagtaaa
    11881 aaacggtgct attattgatt tcgttaggtc agtgtcgcct attctttacc gactttttct
    11941 cagactaatc acgtcagaaa ttccaaactt caaggcttat atttttgata caaagaatga
    12001 ccaatatgat acctggcatt ttcaggccat gttggaatct gatcacgagg ttttcaaggc
    12061 ttacctgtct caaaagcagt ctcgcaatgt gacgaccaaa agcttagcag acgtgttgac
    12121 gttgacctcc ttacctcagg aaatcaagga cttggttttt ttgttacgac attttgaaaa
    12181 ggctgtccgt aatcctctgg ctcatttgat taagcctttt gatgaagagg aactgcatcg
    12241 caccactcat ttttcttctc aggctttttt ggaaaacatt atcaccttgg cgactttttc
    12301 tggtgtaatc taccgatgtg agccttttta ctttgatgac atgaatgcca ttattaaaaa
    12361 ggagttgagc ctttggagac aatctattgt ctgatattta tcaaaaaaca cgataacttg
    12421 accgatacag gtcttactac cgtgtttttt tagtatgacg attgaaaaag aaattaatca
    12481 aaggttactt cttcgagaag gtattcgata aaacgttccc ccattttgga caagttggct
    12541 ttttcatgct tgataaaaac aatgtcaatt tcgtcaggga catctagcgg gatagccaca
    12601 atctggtcac catttagatt gctatttaga ataccactgg caacggtgta gccatctaaa
    12661 ccaatcatta agttaaagag ggtggcacgg tcactgacca caattgattt gctgtggggc
    12721 atctgggaca tcatctcttc tgaaaaataa aaggagttgt gaatcccttg gtcatagctc
    12781 aggtaaggaa aatcgctcaa gtcgtccatg cttaataggc ttttagttgc tagaggattt
    12841 gatttgctga caaaaatatg aggctgtgcc ttaaataatg gagaggcagt caaatgattg
    12901 tcatcaaata atttggtcaa gacatctcgg ttatagtcgt tgataaagag aacaccaatt
    12961 tcagatcgaa agtttttgac atcatcaatg atttcccaag ttcttgtctc gcgcaaaaag
    13021 agttcatacc gagtcatgtc ggtccgcttg aggagtgaga caaatgcatt gactacgaag
    13081 gcataatgtt gagatgaaac gctaaagagc tctcggccag tattgtggtt tttatagcgg
    13141 tcttctaaca gtgatgtttg ctctataatt tgtcgagcat aagataaaaa ttccacccca
    13201 tcttttgtca aggtgatccc tttaggattt cggttgaaaa tggtaatgcc catctccatt
    13261 tccaaatcct tgaccgcatt ggatagactt ggttgtgtga taaataattg cttagcagct
    13321 tcattcatag agccgcactc aacaattttg ataatataat gcagttgttg aattctcata
    13381 agtacagtct agctcaaaaa aaataaagac gcaagcacaa acgtcatcat gggtttagaa
    13441 ctttgaaaaa gcgctggagt cttttctcaa tatggcctag gataaatatg attcaatttt
    13501 gagagttgaa tctatcaatt tcattgccct tttatttttc ttgtgttata ttaatagtat
    13561 taacaaatag gtatttttct aatcaaaggg tggtggtcgt gtgaatgtta ctgaacaaaa
    13621 tagtcatcaa atcttaatcc aaaagttatt ggtcagtatc cactatttga ctctttttcg
    13681 agatgaactg aagcttgtag aacgaactcc ttctatactt ggaggggaat ttccagctca
    13741 tttagtacag tctgagttgg gagatattgt tgcggccatt gataccttgg atatgcagca
    13801 acgcttgatt gagtctacct tttggtatga agaatcagct tttaagttga tgaacaaaac
    13861 tttggatatt gtcgataatt ggattaaagg agtggatcat ctgatagatt tatgccagtc
    13921 caaagaagtc tttcaaatta ttattggaga taaacgcatt cgagtttttg gggtcttaag
    13981 tgatgttttt tcatctttaa aagtgagtgc tttgtcactt aaggaagctc ctattccgga
    14041 tgtgctctat gaacatatcc agagggttaa tcttgaagaa gaaactttta taaaatatta
    14101 cgaaagccct aaggtatcca caggtgagtt tgaataaatg attgggtaaa aatgataaaa
    14161 tgagttgacg taagtataca aaaaaagata tagtaggaaa aatgtgttca aataacaaaa
    14221 agttttgaca tcttgtttta aaaaagctat aatagtttaa atttagacac ctttaagtga
    14281 gtccagagag gcaaacaagg tatacaagat aaaaaaaggg aattcccttt tagtttgctg
    14341 tagcctcgct tctttggtag cgaggctttt tggtgcttaa tgagatattg acacattata
    14401 gaaaggataa gttatgaaag aagaacgccc aattattgct ttagactttt catcatttga
    14461 ggagactaaa gcatttttag acttatttcc agcagaagaa aagctatacg tgaaaattgg
    14521 tatggaatta tattatgcgc aaggaccaga tattgttcgg tctatcaaat cgttagggca
    14581 taatgtattt ttggatttaa aattacatga tattccaaat actgttcgtg ctgctatggc
    14641 agttcttaaa gaattagata ttgatatggc aactgttcat gcagctggtg gtgtagagat
    14701 gctaaaagca gctcgagaag gactaggcca aggccctacc ttaattgcgg taacgcagct
    14761 aacttcaacc agtgaagacc aaatgcgtgg tgatcaaaat attcaaacga gtcttttaga
    14821 gtctgttttg cattacagca agggggcagc taaagctcag ctagatgggg tggtttgttc
    14881 ggcacaagaa gtagaagcta tcaaggcggt gacgcctacg ggatttactt gcttaactcc
    14941 tggcattcgt cctaaaggca gtaatattgg cgatcaaaaa cgagtgatga ctccaaacca
    15001 agcaagacgc attggaagtg attatattgt ggtagggcga ccaattaccc aggcaaaaga
    15061 cccagtggct gcttaccaag ccataaaagc tgagtgggca ggctaacaga taagattagg
    15121 aaaccaacac ttgtctgtta aagaagaaaa aggagaataa gaaatgacac tagcaagcca
    15181 aattgcaact caattacttg atattaaagc tgtttattta aagcctgaag atccttttac
    15241 gtgggcatct ggcattaaat cacctattta cactgataat cgtgtcaccc tttcttatcc
    15301 taagactcgt gatttaattg agaatgggtt tgtggaaact atcagggctc atttcccaga
    15361 agttgaggtc atcgctggta cagctacagc aggtattcct cacggtgcca ttatagcaga
    15421 taaaatgact cttccttttg tttatattcg cagtaagcca aaagatcatg gagcaggcaa
    15481 ccagattgag gggcgtgtgc ttaaaggcca aaaaatggtt atcattgaag atttgatttc
    15541 aacaggtggt tcggtactag atgcagccgc agcagccagt cgtgagggag ctgacgttct
    15601 gggagttgtt gcgattttca cttatgagtt accaaaagct agtcagaatt ttaaagaagc
    15661 tggtatcaag ttgattacgc tttctaatta tactgagcta attgcagttg caaagttgca
    15721 aggctatatt actaatgatg gtttacattt acttaaaaaa tttaaagaag accaggtaaa
    15781 ctggcaacaa taaggaacta tcagccaggt ttatttttaa tgaaaaaatc tctttagccc
    15841 caattcaaaa tgttatgttt tgattgtttt gtctacattc cttacacatc acattctaat
    15901 ttctaacctt ttaatggtgg ttttctttta tttgtgtttt aataaaaaag aatgttttag
    15961 gattcatatc ttagaaagga agagtagcta tgacttatca agatgcgacg gctatggcta
    16021 ttgctgttca gactggtcag accacaccac tagaattagt aacccaggct atttataagg
    16081 ctaaaaagtt gaaccctacc ttaaatgcta ttacctcaga acgctttgaa gcagccctag
    16141 aagaagccaa acaaagggat ttttcaggtc tgccttttgc tggtgtgcct ctttttttaa
    16201 aagatttggg acaagaatta aaaggtcact catcaacctc aggttccaga ttgtttaaag
    16261 agtatcaagc aactaaaacg gatttatttg tgaaaagatt agaagcttta ggctttatta
    16321 ttttagggcg ctccaacacg cctgaatttg gctttaaaaa tattagcgat agcagtttgc
    16381 acggaccagt gaatttacca aaggataata cacgaaatgc tggtggctca tctgggggag
    16441 ctgctgctct agtgtcatca ggaattagtg cgctagcaac tgctagtgat ggtggtggtt
    16501 ctattcgaat tccagcctcg tttaatggct taattgggct caaacctagc cgtgggcgca
    16561 tgcctgtcgg acctggttct tatcgaagct ggcaaggagc ctcagttcat tttgccttga
    16621 ccaaatccgt tagagatact agaaatctac tctactattt acagatggaa caaatggaaa
    16681 gccccttccc cctagccacc ttaacaaaag atagcattta tcaatcgcta caacgtccct
    16741 tgacaattgc tttttatcaa cggttaagtg acggtagtcc agtatcttta gatactgcaa
    16801 aagctttaaa acaagcagta acttggctaa gagaacaagg gcatcagcta gttgaattgg
    16861 aagagtttcc tgtaaatatg acagaagtga ttcgtcatta ttatattatg aatagtgtgg
    16921 aaacggctgc tatgtttgct gatattgaag acacttttgg acgtccgatg accaaagacg
    16981 atatggaaac catgacctgg gctatttacc aatcaggtaa agacatccca gcttggcgtt
    17041 actcacaggt ccttcaaaaa tgggatacgt acagtgcaac catggctagt tttcatgaga
    17101 cttatgacct gttgttgacg ttcacaacca acacaccagc tcctaagcac ggagaacttg
    17161 tcccagattc gaaactcatg gctaacctgg ctcaagctga aattttcagc agtgaagagc
    17221 aattcaattt ggtcgaaacc atgtttggta agagtctggc tattaatcct tacacagctc
    17281 tgcctaatct aactggccag ccagctatta gtttacctac ttacgaaact aaggaagggc
    17341 tcccaatggg cattcaacta attgcagcca aaggtagaga ggacctatta ttaggaattg
    17401 cagagcagtt tgaggcagca ggattattga agattcctca ataataatct tataaaacag
    17461 tttaactatt atagattaac tttttaaaga gattgagcgc tcactgataa cacaacttaa
    17521 tcaaaatgtt atactattct tatcttaata aaggagttac tatgaagaaa tcagttactt
    17581 tactgtctat cggacttgcc agccttttat tggcagcatg tgcgccacat aaatctcaaa
    17641 aatcaagttg ggacactatc aaagaaaaag gggtgttaaa agtagctacc ccaggaacct
    17701 atcagccaac ttctttttac aacgataata atgagttagt aggttacgaa gtagatatgg
    17761 tcaaagaaat cggtaaacga cttaacatta aagtcaagtt tgttgaaaca ggatttgacc
    17821 aagcgtttac ctcagttgat agtggtcgag tggatatttc tttgaacaac tttgacatta
    17881 ccccaaaacg tcagaaaaaa tacaatatct ctacgcctta taaatacggg gtaggaggca
    17941 tgattgtccg tgctgatggc agctcaaaca tggctaaaaa agatcttagc gactggaaag
    18001 gaaaaaaagc agctggtgct tctggaacag aatacatgaa agtagcgcaa aaacagggag
    18061 ccgaactggt gacctacgat aatgttacag gagatgttta tctaaacgat gtggctaacg
    18121 gtcgaacaga tttcatccca aatgattacc cagctcaaaa attatttgtg gattacatgt
    18181 tatctcaaaa tccaaatcta aatgtcaaaa tgagcgatgt tcagtacaac ccaaccgaac
    18241 aaggaattgt tatgaacaaa aaagacgata gcctcaagaa aaaaatagat gctgttatta
    18301 aagacatgat aaaagatggt agcttgaaga aaatatctga aacctactat gctggtcaag
    18361 atttgacaaa accttttggc aaagacaaaa aaattcccgt cattgatacg aaagacgtta
    18421 actaatactt gatgttaaag gagtcctaac cacatgatta atatcccatt aatgaaagat
    18481 agtttaggat ttgtgttatc gggattacct tatacccttg gtatttcgtt gctcagtttt
    18541 tttacgggtc tgtttttagg tttaggccta gcccttctgg gaaggtcacg tcagccgttg
    18601 attcattatc ttgttagagc gtacatctct attatgcgag gtgtgccgat gatcgtggtg
    18661 ctctttgttc tttatttcgg gttgccttat tatggtttag aattgccagc tttactttgc
    18721 gcttatcttg gtttttctat ggttagcgct gcctatattt ctgaagtttt tcgctcttct
    18781 atagaagcta ttgataaggg acaatgggaa gcagctaagg ctttagggtt gccatatgct
    18841 cttatggtta agaaaattat tcttcctcaa gcctttcgaa ttgcagttcc tcctctaggc
    18901 aatgtcatta ttgatatggt taaaagttcg tcgctagctg ccatgattac cgtaccagat
    18961 atttttcaaa atgccaaaat tatcggtggt agggagtggg actacatgtc catgtacatt
    19021 ttagtagcct ttatctattg gattattgct tttttgttag aacgttacca agaattctta
    19081 gaaaataagc tagcacttgt ttaatatcct gtaaaaaacg aatattaacc ctggaaatat
    19141 catattttca gggtctttct atttaaaatg tggtaaacta gatagaaaag gacatcgtat
    19201 ttaacttaga aactgacgtg attagaaagg aatcttatgg ctcattcaat ttggcatgag
    19261 aaaatcaaat catttttgcc ggagcattac tacggtcgca tcaatcactt tttagatgaa
    19321 gcctatgctt ccggtcttgt ctatcctcca cgagaaaatg tctttaaagc cttacaagtc
    19381 actcctttgg aagaaaccaa agtgttgatt ttaggacaag acccttatca tggccccaaa
    19441 caggcccagg gcttatcatt ttcagttcca gaagagattt ctgctccgcc atcccttatt
    19501 aatattttaa aagaattagc agatgacatt ggtcctcgtg accatcatga tttaagcact
    19561 tgggctagtc aaggggttct acttttgaat gcttgcttaa cagtgccagc aggccaggct
    19621 aatgggcatg caggcttaat atgggagcca tttacagacg ctgttattaa agtgctgaat
    19681 gagaaagaca gccctgtagt tttcattttg tggggagcct atgcaaggaa gaaaaaagcc
    19741 ttcattacta atccaaagca ccacatcatc gagagccctc atccaagccc tttgtcatct
    19801 tatcgtggct tttttggcag caaacccttt tcaagaacca acgctatttt agaaaaagag
    19861 ggcatgactg gcgtagattg gctaaaataa gccagccatt tcaactaaat agcttaagct
    19921 agacattttg atacctttaa taagtgaaat gagtgcagta gatttttcct attaaagaac
    19981 gtaaggtctt atggctaaaa cagataaata aaggagaaaa catgatactc atcaaaaatg
    20041 gacgcgttat ggacccgaaa tcgcagagag atcaagtggc agatgtcttg attgatggta
    20101 aacagattgt aaagatagct tcagctattg aatgccaaga agctcaagtg attgatgcta
    20161 gtggtcttat tgttgctccg ggtttggtcg atattcatgt gcattttaga gaacccggtc
    20221 aaacgcacaa agaagatatt cacacgggag ccttggcagc agcagcaggt ggtgtgacta
    20281 cggttgtcat gatggctaat accaatcctg tcatttcaga tgtcgaaact ttacaggaag
    20341 tgctagctag cgcagctaaa gaaaagattc atatttacac caatgccagt gtcacccaag
    20401 cctttaatgg aaaagacgtg actgatttta aagctttgtt ggaagcagga gcagtaagct
    20461 tttcagatga tggtattccc ttagaaagtt ctaaagtttt aaaagaagcc tttgatttgg
    20521 ctaatgccaa ccaaactttc atttctttgc atgaggaaga tcctcagtta aatggggttc
    20581 ttggttttaa tgaaggtatt gctgaggagc attttcattt ttgtggggca actggtgtag
    20641 cagaatatag tatgattgca agagatgtca tgattgctta tgacaggcaa gctcatgttc
    20701 atatccaaca tttatctaaa gcagaatcgg ttcaagtagt ggcttttgct cagcagttgg
    20761 gtgctaaggt aactgctgaa gtgtccccac aacatttttc aacaactgaa gatctcttat
    20821 tgaccgcagg taccagcgcc aagatgaatc ctccccttcg tacacaaaga gatcgcttag
    20881 ctgtcataga aggcttgaaa tcgggtgtta tcacagtcat tgccactgat catgctcctc
    20941 atcacaagga tgaaaaagct gttgatgata tgactaaagc cccatctggt atgacaggac
    21001 ttgaaacttc tttgtcatta ggtttgacgc atttggttga accaggtcat cttacactta
    21061 tgtcactatt agaaaaaatg acgcttaatc cagctttgct ttatggtttt gatgcgggtt
    21121 atttggcaga aaatggtcca gcagatttgg ttatttttgc agacaagcaa gaacgtctga
    21181 tcactgagaa ttttgcttct aaagctagta attccccatt tattggtaac aaactcaaag
    21241 gtgtcgtgaa atacactatt gcagacggag aggttgttta ccctaattaa caaatgagtt
    21301 aaaacaaaaa agcttgactt tcagaggaag tcaggctttt agtaaattta ttttttaggc
    21361 acttgtttgc ttaaatttag cccccaaggt attagatttt cagtatgatg tttgatacga
    21421 ctaatgttat ctttgtgtct tataataata atgaacgcaa gtaatatcac aataaaagtt
    21481 aaaaagtagt cataatttgg taaaagaaaa tgaatggcag gaaatgttaa aacagataac
    21541 acaccaacga tagctgaaac cacactagct aaagatatca tgctaaataa atagagaact
    21601 aaaacaaaga tagatgctaa aaaaaagaga tataacggag caaagcctag caatacacca
    21661 gcacttgttg ctacggcctt accaccttta aagttggcaa aaataggaaa agtatgccct
    21721 aaaactgcga aaaagccgat agcaatggat gaaattgaag tcataccaaa aataattggt
    21781 aacaatattg aaagtgtccc tttaaacata tcaatagcta aggtagctgt tcctgccttg
    21841 acacctaaaa tccgaaaagt atttgtggtt ccagtatttc ctgatccatg ctctcgtaag
    21901 ttgatgtggt aaaagtactg tccaatccat agtccagttg gaatagaacc aagtaaatag
    21961 gcaatggtaa taaaaagtag taatttcata atccttatta taacatattc atagattgga
    22021 gaactagatc tggaaaagaa ttcggaaaaa attagcaaat tattttgcaa aattggtaaa
    22081 aaacttgtaa gataaaaaag atgaaactgt ctggaggtcg gtgttgacta agaaagaaat
    22141 tacaattaat aattataatg acgatgctat ccaagtccta gaagggttag atgctgttcg
    22201 caagcgtcca gggatgtata tcggttcaac agatgctacg ggattgcacc acctgatttg
    22261 ggaaattgtt gataatgctg tcgatgaggc cttgtctggt tttggagatg atattaaggt
    22321 tgttatcaat aaagatggct cggtcagtgt ggcagatagc ggacgtggaa tgccaacagg
    22381 ccagcatgcc atggggattc ccaccgttca agttattttt acgattcttc atgcaggtgg
    22441 taaatttggt cagggtggct acaaaacgtc aggtggtttg cacggtgttg gctcctctgt
    22501 tgtcaatgct ttgtctgcct ggttagaagt cgaaatcaca agagacgggt cagtgtatcg
    22561 tcagcgcttt gaaaatgggg ggaaacctgt tacaaccctt aaaaaagttg gcacagctcc
    22621 taagtctaaa tcaggaacgg ttgtaacctt tatgccagat gataagattt tttcaactat
    22681 tgactttaag ttcaatacca tttcagaacg attgaaagaa tctgcttttc ttttgaaaaa
    22741 tgtcaagatg agcttgacag atcttcgtgg agatgacccg attattgaag agtttcatta
    22801 tgaaaatggg gttcaagatt tcgtcgaata cctcaatgaa gataaggaaa ccttgacacc
    22861 tgttatttac atggaaggtc aagaccaaga ctttcaagtc gaggttgctt tgcagtataa
    22921 cgatggtttc tccgacaata tcttatcctt tgtaaacaat gtgcgcacca aagatggtgg
    22981 aagccatgaa acgggactaa agtcagctat taccaaagcg atgaatgact atgctcgtaa
    23041 aaccaatctc ttaaaagaaa aggataaaaa tctagagggc tcagattatc gagaaggact
    23101 ctcagcagta ttatctatct tagttcctga gcagcacttg caatttgaag gacagaccaa
    23161 agacaaattg ggaagtccct tagcgagacc tattgtagag agcattgttt ctgaaaaatt
    23221 aaccttcttt ttactcgaaa atggagaagt agcttctcat ctagttcgca aagccattaa
    23281 agctagagat gctcgcgaag ctgctcgaaa agcgcgtgat gactcacgta atggtaagaa
    23341 aaacaaaaaa gacaaaggct tgttgtcggg aaaattaacc ccagcccagt ccaaaaatgc
    23401 caagaaaaat gaattgtatc tggtcgaagg agattctgcg ggaggttcag ccaaacaagg
    23461 acgagaccgc aaatttcagg ctattttacc attacgtggc aaagttttaa ataccgaaaa
    23521 agctaagatg gctgacatct tgaaaaatga agaaattaac actatggttt ataccattgg
    23581 agctggagtg ggagctgact ttaatctcga ggatatcaac tatgataaga ttattatcat
    23641 gaccgatgcc gataccgatg gtgcccacat tcaaaccctc ttattgacct tcttttaccg
    23701 ttatatgcga ccattagtag aaacaggtca cgtttatatt gcgcttcccc cgctttataa
    23761 aatgtcaaaa ggtaaaggta aaacagagaa agtgtcctac gcttggacag atggtgaatt
    23821 agaagattta cgtaaggaat ttggtaaagg agccattctt caacgctata aagggctagg
    23881 tgaaatgaac gccaaccaat tgtgggaaac caccatggat cctgaaacca gaacccttat
    23941 tcgtgttact attgatgatt tagcaagagc agaacgacgc gtttctgttt tgatgggtga
    24001 caaagccgct ccacgccgtc agtggatcga ggacaacgtc aagtttacct tagaagaaaa
    24061 tacggtgttt tagtcataca agtttctcac agataattac aagtgctaaa gacgattgtt
    24121 ttttaggtaa ttaataaata atgaaaaaga aagctatttg aatatgtcaa acattcaaaa
    24181 catgtccctt gaggatatta tgggagaacg cttcggccgt tattccaagt atattattca
    24241 ggaacgggca cttcctgata ttcgagatgg attgaaaccc gttcagcgcc gcattcttta
    24301 ttcgatgaat aaggatggta atacttttga aaaaggttac cgtaaatcag ccaaatcagt
    24361 tggtaatatc atggggaatt tccacccgca tggggattcc tctatttatg atgccatggt
    24421 acggatgagt caagattgga agaaccgtga aattcttgtg gaaatgcacg gaaataatgg
    24481 ttcaatggat ggcgatccgc ctgcggctat gcgttataca gaagttcgct tgtctgaaat
    24541 tgcgggttat cttcttcaag acattgagaa aaatacggtc tcttttgcgt ggaactttga
    24601 tgacacagaa aaagaaccca ccgttttacc agcagctttt cctaacttac ttgtcaatgg
    24661 gtcttctggt atttccgcag gttatgcgac agatattcca ccgcacaatt tgtcagaagt
    24721 gattgatgcc gttgtttaca tgattgacca tcccaaggct agcttagaga aattaatgga
    24781 gtttttgcct gggccagatt tcccgacagg aggcattatc caaggagctg acgaaatcaa
    24841 aaaagcctac gaaacaggta agggacgtgt cgttgttcgc tctaggacgg aaattgagga
    24901 acttaaaggt ggtaaacaac aaattattgt cactgagatt ccttatgaag ttaacaaagc
    24961 agtcttggtc aaaaagattg acgatgttcg tgttaacaat aaagtcccag gcatcgtaga
    25021 agtacgtgat gaatctgatc gcacaggtct tcgcatagct attgagttga aaaaagatgc
    25081 agatagccaa accattctaa attatttgtt gaaatacaca gaccttcaag ttaattataa
    25141 tttcaatatg gttgctattg atcactttac accacgccaa gtaggtcttc aaaaaatctt
    25201 atcaagctat atcagccacc gcaaagatat tattattgag cggtctaaat tcgataaagc
    25261 caaagctgaa aaacgcctcc acattgttga aggattgatt cgtgtcttgt ccatcttaga
    25321 tgagattatt gctttgatta gatcgtctga caataaggct gatgctaaag aaaatctgaa
    25381 agtcagctat gatttctctg aggaacaagc agaagcgatt gtaaccttgc agctttatcg
    25441 tttaactaat accgatattg ttactctcca aaatgaagaa aatgatttaa gagacttaat
    25501 cacgaccctt tccgctatta tcggtgacga agcaaccatg tataacgtca tgaaacgtga
    25561 attaagagag gtgaaaaaga aattcgccaa tcctcgttta tcagagttac aagcggaaag
    25621 tcaaatcatt gagattgaca ctgccagctt gattgcagag gaagaaactt ttgttagtgt
    25681 gacacggggc ggatacctca aaagaaccag cccacgatcc ttcaatgcgt ctagtttaga
    25741 agaagttggc aagagagatg atgatgaact catcttcgtt aaacaggcca aaacaacaga
    25801 acaccttttg ttgtttacaa ctttaggcaa tgtcatttac cgaccaatcc atgaattgac
    25861 cgatttacgt tggaaggata tcggtgaaca tttatcacaa accatttcaa acttcgcgac
    25921 agaagaagag attctctacg ctgatattgt gacttccttt gaccagggct tgtatgtggc
    25981 tgtcacacaa aatggtttta tcaaacgctt tgatcgtaaa gaactaagtc cttggcgaac
    26041 ttataaatct aaatcaacca aatatgtgaa gctaaaggat gacaaagacc gcgtggttac
    26101 cttgtcacca gttattatgg aagacctcct attagtgact aaaaatggtt atgctttgcg
    26161 attctccagt caggaagtgc ctattcaagg gttgaaatct gctggtgtta aaggcattaa
    26221 cttgaaaaat gatgacagcc ttgcctcagc ctttgctgtg acaagtaact ctttctttgt
    26281 cttaactcag aggggttctc tcaaacggat ggctgtcgat gatattcctc agacaagtcg
    26341 tgccaatcgt ggactattgg tcctacgaga gttaaaaacc aaacctcata gagtttttct
    26401 ggccggaggc gttcaatcag atacttcagc tgagcagttt gatttgttca ctgatatacc
    26461 tgaagaagaa actaatcaac aaatgcttga agtcattagt aagactggtc aaacctacga
    26521 gattgcttta gaaacgcttt cgttatcgga aagaaccagc aatggctcct ttatttcaga
    26581 cactatttcg gatcaagaag tactggtagc tagaacacgt taaaaagagt gaaaactctt
    26641 tttaatttta tggtaaattt agaatattaa tgcaaaagaa tagaataaac ttgaaaatta
    26701 ttgaaaaaaa gttacaatag aaaaaagcag aaaagaggta cgatgatgac aatagcaatt
    26761 gattgggaca acctaggatt tgaatatcat aagttgccat ttcgttacat ttcttattac
    26821 aaaaatggtc aatgggataa aggtcaatta acagaagatg ccaccttaca tatctcagaa
    26881 agcgctccag cgcttcatta tggacaacaa gcttttgaag gtttaaaggc ttatcgcacc
    26941 aaagatggct ctattcaact atttagacca gaccgtaatg cagcgcgttt acaagccaca
    27001 gcagaccgct tgttgatgcc acaagtatca acagagcaat ttattgatgc agctaagcaa
    27061 gtggttaaag ccaatgaaga ctttgtacca ccttacggaa caggtgcaac gctttacctg
    27121 agaccgttat taattggagt tggagatatt attggggtta aaccagccga agaatatatt
    27181 ttcactattt ttgccatgcc agtaggaaac tatttcaaag gtggacttgc tccaactaat
    27241 tttattgttt ctgaagcgtt tgatcgcgcc gctccttacg gaacaggtgc tgctaaggtg
    27301 ggaggaaact atgcaggaag tttgctacca ggtaaggccg ctaaatcagc aggcttttct
    27361 gatgtgattt acttagatcc agcaacccat actaaaattg aagaagtcgg agctgcaaac
    27421 ttttttggca ttactgcaaa taatgaattt gttacaccac ttagtccatc catcttgcca
    27481 tctattacga agtattcact cttacaacta gctgaagaaa gacttggtat gacagtaatt
    27541 gagggtgatg ttccaattaa tgaattggat aaatttgtgg aagcaggagc ttgtggaaca
    27601 gcagctgtta tctctccaat tggcggtatc cagtacaagg ataacttaca tgtcttttac
    27661 agtgaaacag aagttggtcc tgtgacacgc cgtttatacg atgagttggt cggcattcaa
    27721 tttggggata ttgaagcacc agaaggttgg attgtaaaag tagattaaaa tagagcttgc
    27781 tcttgcaagc ttttttgatt ttgtgtaaaa tagacaaagt gaggtaaagt gatgagtaaa
    27841 aaagataaaa aaattgaaat ccaactgatt gatcacaaag ttatggttaa tgagactaag
    27901 attgatggct atcaattaca aattggtaaa cgtgtggttg gagaaattgc agaacttgat
    27961 gagaaatttg ctgtcttaaa aaatgatgga gtagattgct tcttcaaaac gctcgaacaa
    28021 gcagttgaaa atatcattga aaactacaat ttgaaccatt aaaattagac aaaaacactt
    28081 gccagcttac aaaaactatg atagaatagt ttttgttgtt acggaaagat agcgaagagg
    28141 ctaaacgcgg cggactgtaa atccgctcct tcgggttcgg gggttcgaat ccctctcttt
    28201 ccatttcgga aactaatcta attagaaaat tagttttctg taaaaaaata tttttggggt
    28261 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
    28321 ccccagtcag aggtatcagg tgctccctga gaccgtcaaa gataagagtt gttgtcgttg
    28381 cgggttacct tgagaaaata tattgttatc aaaagctagc attggctagc ttttgttgga
    28441 ggatttttta gaatgaatga atttgaagat ttgctaaaca gtgttagcga agtgaaccct
    28501 ggtgatgttg tcactgcgga agttttaact gttgataacg gtcaagcaaa cgttgttatc
    28561 gaaggaacag gtgttgaagg tgtcttgaca cttcgtgaat tgactaacga ccgcgatgct
    28621 gatattaacg actttgttaa agctggcgac acagttgaag tacttgttct tcgtcaagta
    28681 gtaggtaaag atactgatac agttactttc ctagtatcta aaaaacgttt ggaagctcgt
    28741 aaagcctggg acaaacttgt tggtcgtgaa ggcgaagttg ttactgttaa aggtacccgt
    28801 gctgttaaag gtggtctttc agttgaattt gaaggacttc gtggatttat ccctgcttca
    28861 atgattgaca ctcgttttgt tcgcaacact gaaaaatttg ttggacaaga gtttgatgct
    28921 aaaatcaaag aagttgatgc agcagaaaac cgtttcatcc tatcacgtcg tgaagttatc
    28981 gaagaggccg ctaaagaagc tcgtgctgaa gtgttctcta agatttcaga aggtgcagtc
    29041 gttacaggta ctgtagcgcg tttaacaagt tttggtgctt tcattgacct tggtggtgtt
    29101 gacggacttg ttcacgtgac tgaattgtct cacgaacgta atgtgtcacc taaatcagtt
    29161 gtttcagttg gtgaagaagt tgaagttaaa gttctttcaa ttgacgaaga agctggtcgt
    29221 gtgtcacttt cacttaaagc aacaacacct ggaccatggg acggcgttga acaaaaactt
    29281 gctcaaggtg atgttgttga aggtaaagta aaacgcttga ctgacttcgg tgcttttgtt
    29341 gaagtattac caggtattga tggacttgtt catatttcac aaatttcaca caaacgtgtt
    29401 gaaaatccaa aagatgtact ttctgtagga caagaagtaa cagttaaagt tcttgaagtg
    29461 aatgcagctg atgagcgcat atcattatca atcaaagctc ttgaagaacg cccagcgcaa
    29521 gctgaaggag acaacaaaga agaaaaacgt caatcacgcc cacgtcgtcc aaaacgtgaa
    29581 tcaagacgtg actacgaact cccagaaaca caaactggat tctcaatggc tgatcttttc
    29641 ggtgatattg aattgtaata aatctaaaaa gttgaggttt tcctcaactt tttattgtct
    29701 tctggttaga aaagttattt catattattt cattttatga tatactataa gggtattgac
    29761 aaagttgtga ccacctttag tgtaatggat atcacgtaag attccggttc ttgagatggg
    29821 ggttcgattc cctcaaggtg gatagaaatc atataaaaaa gccctatttg ataggctttt
    29881 tttgtttctt ttccaaaatt tgtccaaaat tatctagaag agttgacgaa ccttttcaaa
    29941 atcattattt ttctgacctt gaaaacgatg agtataggtt ttaaatatct ctgtaatgcc
    30001 acagcctgaa ttttatttga ctcagattca taagattgta agtaatcaag gttaattatt
    30061 gatgagatgg tgaaaccatt tttagcaagt gctattaaag catttagtga tttttcgaaa
    30121 cgttttaaaa tttaagaaga gttttaccta aaaaactagc tgtgtcatct gtctaagcac
    30181 agttagtttt ttagtattag atttttaaag aagaaacgtc tcaatcttaa ttggataatc
    30241 cttatatttg gattttaata tagtagctac ttcttgatat agtatccgaa tctgcgctct
    30301 tttttcaggc atgttaaaac caacataatg gaattgtgat tcaatagtta caaataaatc
    30361 atggaacagg tgattcatag tttctagttt atggcaaagc agtcgatctt cttgaatgaa
    30421 tgttggggga ttttgatctt ctccccaata agcataaaca cgtgacaacg gataagtccc
    30481 atattctaaa caaagttcat aagtcatttt ggcctccttg ttttatctta gcattttcta
    30541 aataaagctt ctaatgatct gcttaaagga gtaatggatt gcatctcatc tcatcaaaaa
    30601 taaattgaga tctattttgt gaaaaaaaag acaaacaaat gacttgccta gacttgtttt
    30661 tcctagatat aataggaagt aactgaataa caatgaagga gaaaaaatgt cagaaaaaat
    30721 cagagtttta ctctattata aatatgtttc aattgaaaat gcgcaagagt acgctgcgaa
    30781 acatctagaa ttttgtaaat ctattgggct taaaggtcgt attttgattg cggatgaggg
    30841 gattaatgga actgtttctg gagattatga aacaactcag aagtatatgg attgggtcca
    30901 cagcgatgag cgttttgctg atctttggtt caagattgac gaggaaaatc aacaagcttt
    30961 ccgtaaaatg tttgttcgtt ataagaaaga gatcgtgcac cttgggttag aagacaataa
    31021 ctttgatagt gatattaatc ctttggaaac aacaggtgag tatttaaatc ctaaacaatt
    31081 taaagaagcc ttgcttgatg aagatactgt tgttttagat acgcgtaatg attatgagta
    31141 tgacttaggt cacttccgtg gagcaattcg tcctgatatt cgcaatttcc gtgagttacc
    31201 acaatgggta cgtgacaaca aagataagtt tatggaaaaa cgtgttgttg tgtactgtac
    31261 gggtggtgtt cgctgtgaga aattctcggg ttggctggtt cgagaaggct ttaaagatgt
    31321 tggacaatta cacggtggta ttgcaacata tggaaaagat ccggaagttc aaggtgaact
    31381 ttgggatggt gcgatgtatg tttttgatga tcgtatctcg gtgccaatta atcatgttaa
    31441 tccaactgtc attagtaaag attattttga tggaacacct tgtgaacgtt atgtcaattg
    31501 tgcaaatccg ttttgtaaca aacaaatttt tgcttcagaa gaaaacgaaa caaagtatgt
    31561 tcgaggatgc tcgcctgaat gtcgtgcaca tgaacgtaat cgctatgtgc aagaaaatgg
    31621 tttatcacgt caagaatggg cagaacgttt agaagctatt ggagaatcct tgccagaatt
    31681 tgtaggtgcc taatatctca aaacaaatca ggaaactggt ttgttttttg tgatacagat
    31741 gatgagacca aattgcaaag actttgcaac aagatactag tgaacgtgga caccaagctc
    31801 attttgtcat gcagagccat caacagcgaa gacgtcaaca ccaactctgt ctagaacctt
    31861 ttcaaaagaa ttgtgagttt ctgtattacg agatagagaa ggtaaaactg gattctgaag
    31921 actgccttac catcaatcct ttgggaatgg tttaagcttt acagcttgat gatggtcaaa
    31981 tcgtcacaca ggcaggtatt atcttgctct ttatagcgaa acaaaccctg aaatctcagc
    32041 tcattcccca agataatctt ttggcagaaa gttgattcaa cgaaatcatg gatttcttaa
    32101 caggtgattt tccccttgtt tttaggccaa tgtacaaccc tcatcgctac actatttctc
    32161 aagacaatca agcgcttgaa aaagtcaaac aagcttctta caagcggatg gatattgcta
    32221 tgacgcattt agatggtctt attggggata gtgggcatgt ttatcgagat caacaaacca
    32281 ttgcagatgc ttatgcttac gccatggcgc tatggtctca aaaaacacca aaatcttacg
    32341 agaattatcc gcatct
//
LOCUS       DAUGHY010000018        31768 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.018, whole genome shotgun sequence.
ACCESSION   DAUGHY010000018 DAUGHY010000000
VERSION     DAUGHY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31768
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.018"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            12..2744
                     /locus_tag="VH752_000805"
     CDS             12..2744
                     /locus_tag="VH752_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnA family nuclease"
                     /protein_id="HEQ8289167.1"
                     /translation="MINKKYIIPVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKR
                     ESVISDKSNFPVISPYLASVDFGERKTPLPTPDKGVKVTTEQSIAQVRKGPEERTYTV
                     TGKITSVINGWGGYGFYIQDSEGIGLYVYPEKDLGYSKGDIVQLTGTLTRFKGDLQLQ
                     QVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDD
                     SGKSIVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKK
                     VTSSNSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDG
                     DLATSDGIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVTKT
                     GTAQVPSPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGP
                     MKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAG
                     PVSYSYGNYKVFVDDSKNMPSLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSST
                     KDEKVKRIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGP
                     TYRYVDIAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSV
                     GRIDPTNAAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGRVQPVTFKSEQR
                     RHVLANMLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVSRHDISDRY
                     SYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKEN
                     DKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      2909..3024
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            3022..3522
                     /locus_tag="VH752_000806"
     CDS             3022..3522
                     /locus_tag="VH752_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEQ8289168.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
     gene            3716..5674
                     /gene="ligA"
                     /locus_tag="VH752_000807"
     CDS             3716..5674
                     /gene="ligA"
                     /locus_tag="VH752_000807"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEQ8289169.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTSVVAKRQLATFLYQE
                     ASPTARNQQNEVLAELAGLGFSVNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKENFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRQL"
     gene            5688..6710
                     /locus_tag="VH752_000808"
     CDS             5688..6710
                     /locus_tag="VH752_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ8289170.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEIEEIVKKFAHEVEDL
                     EQELEE"
     gene            7102..7299
                     /locus_tag="VH752_000809"
     CDS             7102..7299
                     /locus_tag="VH752_000809"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEQ8289171.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            7334..8050
                     /gene="atpB"
                     /locus_tag="VH752_000810"
     CDS             7334..8050
                     /gene="atpB"
                     /locus_tag="VH752_000810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEQ8289172.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAVIFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            8068..8562
                     /gene="atpF"
                     /locus_tag="VH752_000811"
     CDS             8068..8562
                     /gene="atpF"
                     /locus_tag="VH752_000811"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEQ8289173.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRSQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            8562..9098
                     /locus_tag="VH752_000812"
     CDS             8562..9098
                     /locus_tag="VH752_000812"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEQ8289174.1"
                     /translation="MTKKEQALIEQYAKSLVEVASEHHSLDALQADVLAILETFVTTN
                     LDQSLSSLAVPHAEKIKLLTLLKGNNSVYMNNFLNLILQNEREAYLYQMLQAVLNEIA
                     IVSNQYDVTVTSSLPLTEEQKSRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            9114..10622
                     /locus_tag="VH752_000813"
     CDS             9114..10622
                     /locus_tag="VH752_000813"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEQ8289175.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            10638..11513
                     /locus_tag="VH752_000814"
     CDS             10638..11513
                     /locus_tag="VH752_000814"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEQ8289176.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            11675..13081
                     /gene="atpD"
                     /locus_tag="VH752_000815"
     CDS             11675..13081
                     /gene="atpD"
                     /locus_tag="VH752_000815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEQ8289177.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVAETVRGFKEILEGKYDDLPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            13094..13510
                     /locus_tag="VH752_000816"
     CDS             13094..13510
                     /locus_tag="VH752_000816"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEQ8289178.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKCISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTVVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            13776..14033
                     /locus_tag="VH752_000817"
     CDS             13776..14033
                     /locus_tag="VH752_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEQ8289179.1"
                     /translation="MVCYNNPMELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYH
                     PENLGRLRLFVFLLATALGYLVSHCMLELLQISQTLFFEFC"
     gene            14098..15369
                     /gene="murA"
                     /locus_tag="VH752_000818"
     CDS             14098..15369
                     /gene="murA"
                     /locus_tag="VH752_000818"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ8289180.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDIAAQADRLQGAMI
                     YMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLT
                     ITGVTSLHGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLMEMGVS
                     VTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFEN
                     RFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTI
                     VNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
     gene            15373..15561
                     /locus_tag="VH752_000819"
     CDS             15373..15561
                     /locus_tag="VH752_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ8289181.1"
                     /translation="MTNGLKYVLEQMLLLLVIAALACLFLAIGLMIGYSFMGDGHSPW
                     HILSMDKWAELVNKFTGK"
     gene            15597..16136
                     /locus_tag="VH752_000820"
     CDS             15597..16136
                     /locus_tag="VH752_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEQ8289182.1"
                     /translation="MLTRKKRKRVQEQKVPKRANALLTKSTRQYQDRSQLKKGYRSFK
                     PAGWHRLHHLKGSYDHAVDRGHLLGYALVGGLKGFDASTGNPDNIATQLSWANQANKP
                     YSTGQNYYEGLVRRALDKGHRVRYRVTLLYDGDNLLASGSHLKAKSSDDSLAFNVFVP
                     NVQAGLTVDYRTGQIAINL"
     gene            16419..17462
                     /gene="pheS"
                     /locus_tag="VH752_000821"
     CDS             16419..17462
                     /gene="pheS"
                     /locus_tag="VH752_000821"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEQ8289183.1"
                     /translation="MDLQAQLEELKSKTLETLQSLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQI
                     EGLVVGKNISMGDLKGTLEMIIKKMFGEERSIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDQRFSEQFN"
     gene            17672..20077
                     /locus_tag="VH752_000822"
     CDS             17672..20077
                     /locus_tag="VH752_000822"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEQ8289184.1"
                     /translation="MLVSYKWLKELVDIDVTPVALAEKMSTTGIEVEGIEVPAEGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIELSIASDN
                     VLTYASRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYLGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVAEKPVALAGVMGGQATEIDGNS
                     QTVVLEAAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYGYDKLPTTLPEAGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVSGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKEDLPNEVNHFAFAICGLVAQKDFQTQAQAVDFYHA
                     KGILDTLFANLNLKVQYVPTKDLANMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELDMAALEAALPSDQTFAEITKFPAMTRDVALLLDREVSHQAIVTAIESAGVK
                     RLTSIKLFDVYEGATIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKALTEQVGA
                     EVR"
     gene            20187..20564
                     /locus_tag="VH752_000823"
     CDS             20187..20564
                     /locus_tag="VH752_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEQ8289185.1"
                     /translation="MYQTKITGNRLYHTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTVAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVTVTITLEGLSHV"
     gene            20557..20943
                     /locus_tag="VH752_000824"
     CDS             20557..20943
                     /locus_tag="VH752_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEQ8289186.1"
                     /translation="MSDASLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFHCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKDHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            21016..22092
                     /locus_tag="VH752_000825"
     CDS             21016..22092
                     /locus_tag="VH752_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289187.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAVDLWKADSVLLAKDADATLTLSQVSRAQENQITADKVAPLAQLNTVAWSVKNPKDA
                     DKVKVRLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGEPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTFITKLPGYSAQLLTFGFMISFLVIISAIIIGIFMYILTIQKAPIFGIMKAQ
                     GIGNITIAQAVLLQTFLLSALGSGLGLLGTWLTSLVLPEAVPFQSNWLVYLAILVSMV
                     CFALLGTLFSVFSIVRIDPLKAIG"
     gene            22102..22770
                     /locus_tag="VH752_000826"
     CDS             22102..22770
                     /locus_tag="VH752_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289188.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGGLQTPSSGQLIIDGTDYTHLSEKERSRLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGIAGLKHQLPQELSGGERQRAAIARALYHDPA
                     LILADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLKYCDKVYRMQDGEL
                     RQER"
     gene            complement(22874..23791)
                     /locus_tag="VH752_000827"
     CDS             complement(22874..23791)
                     /locus_tag="VH752_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEQ8289189.1"
                     /translation="MKTDDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWK
                     TKLVILLLLLVMGGGGLSGVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVF
                     ASTEDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISF
                     YNELSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASTRQGKSKAKANQLNVKLE
                     LQADYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRAVPDSFTHGTS
                     KQRQRWFDRGYQYGDFEHGDTFSIPYSKL"
     gene            23942..27157
                     /gene="rexB"
                     /locus_tag="VH752_000828"
     CDS             23942..27157
                     /gene="rexB"
                     /locus_tag="VH752_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEQ8289190.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRALMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVIVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKNDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLIDKLILFLEKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     HIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDQERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVPTVINETFDDLSPYLKELINFGLPLLDKGKNYLSYDNSDIGNYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIKVIDQE
                     KNFHLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLVTYLA
                     ALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQIDNTLVESIYKALTYKGIFSEVEKE
                     HLSTGAYQTKNALYSNDELETLLNYNKYLYLKAAKHIKKGHFLINPYTSDGKTVQGDQ
                     LKAITRFEADLDMAQARRLVTLPAKEKKECFLTLMRKESHL"
     gene            27118..30786
                     /gene="addA"
                     /locus_tag="VH752_000829"
     CDS             27118..30786
                     /gene="addA"
                     /locus_tag="VH752_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEQ8289191.1"
                     /translation="MLFNINEKGEPLVISFAPFLSPEAIKHLQENERCSDQSQKRTAQ
                     QIEAIYTSGQNILVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRE
                     RIENKLYSQIAQTTDFQMKVYLTEQLQSLGQADIGTMDAFAQKVVSRYGYSIGISSQF
                     RIMQDKAEQDVLKQEVFSKLFSEFMNQKEAPVFRALVKNFSGNCKDTSAFRELVYTCY
                     SFSQSTENPKIWLQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDY
                     GQLTKAGSRSAKYTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTGLIPSGNDVTV
                     SKVKYPVFKTLHQKLKQFRHLETILMYQKDCFPLLEQLQDFVLAFSEAYLAVKIQESA
                     FEFSDITHFAIKILEENTDIRQSYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFM
                     VGDIKQSIYRFRQADPQIFNQKFRDYQKKTEQGKVILLKENFRSQSEVLNVSNAVFSH
                     LMDESVGDVLYDEQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFSE
                     VTIVAKEIIKLHNDKGVPFEDITLLVSSRTRNDIISHTFNQYGIPIVTDGGQQNYLKS
                     VEVMVMLDTLRTINNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKI
                     QRAVIGRGAHPELIHDTLLGKLNIFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFV
                     ASQAKAEQAQANLYALALRANQFEKSGYKGLYCFIKMIDKVLETQNDLADVEVAAPKQ
                     AVNLMTIHKSKGLQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGE
                     TTLNSVKVSMETLPYQLNKQELRLATLSEQMRLLYVAMTRAEKKVYFIGKASKSKSQD
                     ITDPKKLGKLLPLALREQLLTFQDWLLAIADVFSTEDLYFDVRFIEDSDLTQESVGRL
                     QTPQLLNPDDLKDNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKAAYEP
                     LLEPIGVDIIEKSSRSLSDFTLPHFSKKVKVEASHIGSALHQLMQVLPLSKPINQQTL
                     LDALREIDSNEEVKTALDLKKIESFFCDTSLGQFFQTYQKHLYREAPFAILKVDPISQ
                     EEYVLRGIIDAYFLFDDHIVLVDYKTDKYKQPIELKKRYQQQLELYAEALTQTYKLPV
                     TKRYLVLMGGGKPEIVEV"
     gene            30926..31738
                     /locus_tag="VH752_000830"
     CDS             30926..31738
                     /locus_tag="VH752_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ8289192.1"
                     /translation="MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAV
                     SPDYAPFEFKALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKAD
                     LAISGISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIE
                     EGLVKIQLPKANLISLTAMGEAINELKAGQVYAVTLEAPVAAGFLAQHKDLALAPFSL
                     KTSDGDAKAVALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
BASE COUNT         9957 a         5537 c         6639 g         9635 t
ORIGIN      
        1 aaggagatat catgattaac aagaaatata taatacctgt ttcattgttg acactagcta
       61 ttacgcttac tagtgttgaa gaagttactt cacgccaaaa tttgacttat gccaatgaaa
      121 tcgtaacaca aaggccaaag agagaatctg ttattagtga taaatcgaat tttcccgtca
      181 tatcacctta cctagcaagt gtggattttg gtgagagaaa aacacctttg ccaacacctg
      241 ataaaggagt aaaagtaact actgaacagt ctattgctca agtaagaaag gggcctgaag
      301 aaagaaccta tactgttact ggcaagatta cgagtgtgat caatggctgg ggaggctatg
      361 gcttttatat tcaagatagt gaaggtattg gactttatgt ttatcctgaa aaagatttag
      421 gatacagtaa gggagatatt gttcaattaa caggtacact tactcgcttt aaaggtgatt
      481 tacaactcca acaggtgact gcacacaaaa agttagagtt atcttttccg acttctgtta
      541 aagaagcagt aatatcagaa ttagaaacaa caacaccctc aacattagtt aagttatctc
      601 acgtgacagt tggagaatta tcaactgatc aatataacaa cacatctttc cttgtaaggg
      661 atgatagtgg taaaagtata gttgttcata tagatcatcg tacaggggtt aaaggggctg
      721 atgttgttac taaaataagt cagggtgatt tgattaacct cacagccata ttgtctattg
      781 ttgatggtca gttacaatta agaccgtttt ctcttgaaca attggaagtg gttaaaaagg
      841 tcacaagctc aaatagtgat gcttcatctc gtaatattgt gaaaataggc gagattcaag
      901 gagctagtca tacgtcgcca cttctcaaaa aagcggtcac cgtagaacag gttgttgtca
      961 cttatttaga cgattccact catttttatg ttcaagatct taatggtgat ggtgatttag
     1021 cgacttcaga tggtattcgt gtttttgcta aaaacgctaa ggttcaagtc ggcgatgttt
     1081 tgaccatttc aggtgaagtg gaagaattct ttggtcgtgg ttatgaggaa cgtaagcaga
     1141 ctgaccttac catcacccaa attgtggcta aagcagtgac caaaacaggg acagctcaag
     1201 ttccatcacc gcttgtttta gggaaagatc gtatcgcgcc agccaatatt attgataatg
     1261 atggcttgcg tgtgtttgat ccagaagaag acgctattga ttattgggaa tcaatggaag
     1321 gcatgttagt ggcggttgat gatgctaaaa tccttggtcc aatgaaaaat aaagaaattt
     1381 atgtcttacc tggctctagt acaagaccgt taaataattc aggtggagta ttacttccag
     1441 ctaattctta taacacagat gtgattcctg ttcttttcaa aaaaggcaaa caaattatta
     1501 aagcaggaga ctcttacaaa ggaagattag ctgggccagt atcttatagc tatggtaatt
     1561 acaaggtctt tgttgatgac agcaaaaaca tgccaagttt aatggatggt catctaaaac
     1621 ctgaaaaaac aaacttgcaa aaagacctta gcaagttaag cattgcttct tacaatattg
     1681 aaaacttctc agccaatcct tcttcaacta aagatgagaa ggtcaaacgg attgccgaat
     1741 cctttattca tgatctgaat gctccagaca ttattggatt aattgaagtc caagataata
     1801 atgggccgac tgatgatggg acaacggatg cgacacaaag cgcacaacgc ctcattgatg
     1861 ctattaaaaa actaggtggc ccaacttatc gttatgttga tattgctcca gaaaataatg
     1921 ttgacggagg tcaaccaggt ggtaatattc gaacaggatt cctttatcaa ccagagcgcg
     1981 tcagcctttc tgataagcca aaaggcggtg ctcgtgatgc tctaacttgg gttaatggag
     2041 aattaaacct tagtgttggt cgaattgatc caactaacgc cgcttggaaa gatgttcgta
     2101 aatcactagc agcagaattt atcttccaag gtcgtaaagt cgttgttgtt gcaaatcatt
     2161 tgaactctaa gcgtggggat aatgctcttt atggtcgtgt gcaaccagtc acttttaaat
     2221 ctgagcaaag acgtcacgtc ttggctaata tgctagcaca atttgcgaaa gaaggggcaa
     2281 aacaccaagc taatattgtg atgctaggtg actttaatga ttttgaattc acaaagacga
     2341 ttcaattaat cgaagaaggt gatatggtta acttggtgag ccgacatgat atttcagatc
     2401 ggtattctta ttttcaccaa ggcaataatc agacccttga taatatatta gtttcacgcc
     2461 atttacttga tcactacgaa tttgacatgg ttcatgtgaa ttccccattt atggaagctc
     2521 acggacgcgc atcagatcat gatccattgt tacttcaatt atcattttcc aaagaaaatg
     2581 ataaggcaga gtcttctaaa caaagtgtaa aagctaaaaa aacttcaaaa ggaaaactgt
     2641 tgccaaaaac aggagatagt cttgtttatg tgataacgct actaggaacg gctagtttat
     2701 tagtgcctat tttattattg actaaaggca aaaaggaatc atagcgattt gaagaattgg
     2761 agttcataaa gagtcgttaa gggcttgata tacttacgtt agaccttaag ctaaaatgga
     2821 tgactatata aaaactaata ttgataacaa aagaagataa caataagaca gctttacttg
     2881 ctaaagactt ttgtcaagct aattgtttta tggtacaatg taaccgttgc tttgcaaccc
     2941 ttggtgctta gttccctttc accaagcata ttagaaacgt agatagcttt tgagagttat
     3001 ccagacgtta aaggagaaaa aatgacaaaa ttgactgtac acgactatgt tcacattggg
     3061 ctagtagctg ccttgtatgt tgtcttaact attaccccgc ctttaaatgc gatttcttat
     3121 gggatgtatc aatttagaat atctgaaatg atgaatttct tagcttttta tcatcgcaag
     3181 tatattattg cggtgactct tggatgtatg attgctaact tttatagttt cggactgatt
     3241 gatgtctttg taggcggcgg ttcaactctt atttttgtga ccttaggtgt tattttgttt
     3301 agtaagtatc aaaaagatta tctttttaat ggcattttta ataaagcttt tgtttacttt
     3361 tctttctttt ttgcaacttc catgtttact gtagcaatcg aactttactt ctttggagct
     3421 ccatttttat taacatggtt tacaactgcg ttgggagaat tagtatctct gctcatagga
     3481 tcacttatta ttgataagtt gtctcaaaga atttcctttt agtaatgcat catactcaaa
     3541 agagttgggt caataccctt ctctttttta tgttgtttgt aatgttaaaa tcgttctaga
     3601 tagaaatatt taacacttta gctaatagta ccatatggag tggccaacga ttacttttga
     3661 tgttgttatg atatagaaat agctcatttg ttatctttta tgataaaata aaagtatgaa
     3721 aaaacgcata aaagaattaa cagatcttct taaccgttac cgttatgact attatactaa
     3781 agatgcccca tcagtttctg acagtgacta tgataagctt tatcgtgaac tggtgacttt
     3841 agagcagtca tatcccgagt atgtcttgca agatagccca acgcagcagg ttggagggac
     3901 tattttaaag gggtttgaaa aatatcgaca tcagtatccg ctttttagtt tgcaggatgc
     3961 tttttcacgt gaggagctgg atgcttttga taagcgcgtc aaagccgaat ttccaaatgc
     4021 cacctatcta gcagagttaa aaattgatgg tttatctatt tccttgagtt atgagaatgg
     4081 cttcttacag gttggagcaa cacggggtga tggcaatatc ggtgaaaata tcacagaaaa
     4141 tatcaaaaaa ataaaggata ttcctcacca attgagtgag cctttaacca tcaccgttcg
     4201 aggagaggct tacatgtctc gtcagtcctt taaagcgatc aatgaagcac gccaagaaaa
     4261 tggggagact gaatttgcta atcctagaaa tgctgccgct gggacactaa ggcagctaga
     4321 tacgtcagtg gttgctaaac gtcaactagc cacttttttg tatcaagaag ctagtccgac
     4381 agctagaaat caacaaaatg aggtcttagc agaattagca ggtctaggtt tttcagtaaa
     4441 tccgtattat caactcacat cgtctatgga tgagatttgg gattttatca aaactattga
     4501 ggcaaaaaga gatcaattag cttatgacat agatggcgtt gttattaaag ttaatagcct
     4561 agccatgcaa gaagagctag ggtttacggt taaagctcca cgctgggcca ttgcttacaa
     4621 atttccagca gaagaaaaag aggcagaaat cctttcggtt gactggacag tgggacgaac
     4681 aggcgtggtc acacctacag cgaatctaac tcctgtgcaa ctagcgggga caaccgtgag
     4741 tcgtgcaacc ctacataatg tggattatat tgccgaaaaa gatatccgta ttggcgatac
     4801 agtgattgta tataaggcag gggacattat tcctgctgtc ttaaatgtcg ttatgagtaa
     4861 gcggaaccag caagaagtca tgcttattcc taaactatgc ccatcttgtg gtagtgaatt
     4921 agttcatttt gaagatgagg ttgctttgcg ttgtattaat ccgttgtgtc ctagtttgat
     4981 tcagcgaagt ttggagcatt ttgcaagtcg tgatgccatg aacattactg ggctgggacc
     5041 agctattgtg gaaaaactat ttttagcggg gtttgttcat gatgttgctg atatttacca
     5101 gttgaccaaa gaaaatttca tgcaattaga tggcatcaaa gaaaagtcag ctgataaatt
     5161 gctcgctgcg attgaggctt ctaaatcaaa ttcagctgaa aaactgttat ttggtctagg
     5221 aattcgtcat attggctcta aagttagtcg cttaatttta gaggtatacg gagatatttc
     5281 tgctctttta accgctaaag aagaggagat cgcaaggata gatggccttg gctcaacgat
     5341 tgctcaatca cttactcaat actttgaaca aaaaacggct gcgattttag tagacgagtt
     5401 aaagacagca ggtgtcaaca tgcattactc aggtcaaaaa gttaatagtg acgcagcgct
     5461 ttttggtcta actgttgttt taactggcaa attaaatcag ctgaaccgaa atgaggccaa
     5521 agataaatta gaggcattag gtgccaaagt tactggaagt gtatctaaga aaacagattt
     5581 agttattgca ggtagtgacg ctggttctaa gctagaaaaa gcaaagagct taggaattcg
     5641 gattgaagat gaagattggc tacgccagct ttaagtgagg taatgttatg aaaaaacaat
     5701 tgagagcacg tctcatttat aatccaacgt caggacaaga acttatgaga aaaagtgttc
     5761 ctgaagtttt agacatatta gaagggttcg ggtatgaaac atctgccttt caaacaacag
     5821 cccaaaagaa ctctgctcta aatgaagcaa gaagagcggc aaaggcaggt tttgacttat
     5881 tgattgctgc tggaggtgat ggcactatta atgaagtggt caatggaatt gctcccttga
     5941 aaaagcgccc taaaatggcc attattccaa caggtaccac taatgatttt gctagagcgt
     6001 taaaagtacc aagaggaaat ccgagccaag cagcaaaact tatcggaaaa aatcagacta
     6061 tccagatgga tattggtaga gctaagaaag atacttattt catcaatatt gctgcagcag
     6121 gaagtttgac ggaattaact tacagtgtac caagccagtt aaaaacgatg tttggatact
     6181 tggcttatct tgctaaagga gtagaactgc tcccaagagt tagcaatgtg cctgttaaaa
     6241 ttacgcatga caaaggcgtc tttgaaggtc aagtttctat gatttttgca gctattacca
     6301 attctgttgg gggatttgaa atgattgctc ctgatgcaaa actagatgac ggtatgttta
     6361 cgctcatttt aataaagaca gcaaatcttt ttgaaattgt acatcttttg cgtttgattt
     6421 tggatggtgg taagcatatt acagatcgcc gtgtcgaata cattaagacc agtaaaatag
     6481 tcattgaacc ccaatgtggt aagcgaatga tgattaattt agatggtgag tacggaggag
     6541 atgctccaat tacattagag aacctaaaaa atcatatcac tttttttgca gatactgatt
     6601 tgatttctga tgacgcttta gtcttagatc aggacgaatt ggaaattgag gaaatcgtaa
     6661 agaaatttgc tcatgaagta gaagacttag aacaagaatt agaagaataa tacaatagtt
     6721 attagctact gacagtttag tcaaatgcac tactattaga atctaagatt tttaaatcaa
     6781 ttgaggtagt gttttttgat gcactcaaag agtcaattta ttcatatgag gggcctattt
     6841 gaggtctctc ttttttgatc atatgcaaga aaatgccatg ttttaggggt gttattatac
     6901 cctttttgga atcttgttgc ctttttgtta taataagagt gtaattctat ttttaaaaag
     6961 atcttaacaa agacagactc gtttatcaaa aaggttagta ttagtctaag aaagcgtact
     7021 cagtctaaag aaatctgttg acattcttaa ttttaaggaa tagaatagca aagtaaaaaa
     7081 aatttaaagg agtattctac tatgaatcct attttcgcat tggctttggc ctgttttggt
     7141 gtttctcttg ccgaaggttt cttgatggca aatcttttta aagcagcatc tcgccaacca
     7201 gaaatcattg gtcaattacg ttcattaatg atcttgggtg ttgcctttat cgaaggtact
     7261 ttctttgtta cccttgttat ggccttcatt cttaaataac agcctatttg ataatagaaa
     7321 aggagggtgt tagatggaag aagctaaaat acccatgcta aagcttggtc caataacctt
     7381 taatttgacc ctacttgctg tttgtattgt cacaattgct gttatctttg cctttgtttt
     7441 ttgggcaagt cgccaaatga aattgaaacc agaagggaag caaactgctt tagaatattt
     7501 aatcagtttt gtggatggta ttggagaaga gcacttagat cataatctac aaaaatctta
     7561 ttcgctgtta ctctttacca tttttctctt tgtggctgtc gctaataatt tagggttatt
     7621 tactaagtta gagacagtta atggctataa cctatggacc tcgccaacag ccaatctggc
     7681 ttttgacctt gctctatctc tttttattac cttaatggta cacattgaag gggttagacg
     7741 tcgtggcttg gttgcccatt tgaaacgttt ggctacaccg tggccaatga ctccgatgaa
     7801 tttattagaa gagttcacaa atttcttatc acttgccatt aggttattcg gtaatatctt
     7861 tgccggggaa gttgttacag gtttgattgt tcaactggcc aattatcgag tttattggtg
     7921 gccgatcgct ttcctagtca atatggcgtg gacagccttt tcagtcttta tttcctgcat
     7981 acaggctttt gttttcacaa aactaacagc gacctatcta ggaaagaaag ttaatgaatc
     8041 agaagaataa ggaaggagaa caaacgaatg tcaatcacat ttggtgaact cgttgggaat
     8101 tttattctag taacaggttc tgtaatcgtc ttgttactct tgatcaaaaa attcgcttgg
     8161 ggtgcaattg aatcgatttt acagacacgc tcacaacaaa tctctcgaga tattgatcag
     8221 gctgagcaat cacgtctaag tgctcaacag ttagaggcaa aaagtcaagc taacctagat
     8281 gctagtcgtt cacaagcaag taaaatcatt agtgatgcca aagaaattgg tcaattacaa
     8341 ggtgataaat tggtggcaga agctactgat gaagcaaaac gcttgaaaga aaaagcgttg
     8401 acagatattg aacaaagcaa atcagacgct atttcagcag tcaaaacaga aatgtctgat
     8461 ttaacggttc ttttagcgga aaaaattatg ggagccaatc ttgataagac ggcgcaaagc
     8521 cagcttattg acagttatct tgatgactta ggagaagctt aatgaccaaa aaagaacagg
     8581 ctcttatcga gcaatatgca aaaagccttg ttgaggtggc aagtgaacat cattctcttg
     8641 atgccttaca agctgatgtg cttgcgattt tagagacgtt tgtaacaact aatcttgatc
     8701 aaagtctatc aagtctagct gttccgcatg ctgaaaaaat aaaattgcta accttattaa
     8761 agggaaataa ttctgtttat atgaataatt tcttgaatct cattttacaa aacgaacgtg
     8821 aagcttattt gtatcaaatg cttcaggctg ttttaaatga aattgcaata gtttcaaatc
     8881 agtatgatgt gacagttacc tcatcactac ctttgactga ggagcaaaaa agccgtgttc
     8941 gagcagttgt tgcaaagaaa tttgctgtaa cagcgggtcg tttaattgaa aaagtagatc
     9001 cttcgcttat tggaggattt attatcagcg tcaataataa agtaattgat accagtattc
     9061 gtcgtcaatt gcaagcattt aaaatgaatt taaaatagaa agtggtgtga cttttggcaa
     9121 ttaatgcaca agaaattagc gctttaatta aaaagcaaat tgagaacttc cagccaaatt
     9181 tcgatgtcac agaaactggg atcgttacct atattggtga cggtattgca cgtgcccgtg
     9241 gattagataa tgccatgagt ggtgaacttc ttgaatttga aaatggtgcc tacgggatgg
     9301 ctcaaaacct tgagtcaaat gatgtgggta ttattattct tggtgatttt tcagcaatcc
     9361 gtgaaggaga tgtggttaag cgcaccggta aaatcatgga agtaccagtt ggtgaagctc
     9421 tcattggacg tgtggtaaac ccacttggac aaccagttga tggcttggga gatattgaaa
     9481 caactggctt tagaccagta gaaacgccag ctcctggtgt tatgcagcgt aaatccgttt
     9541 ctgaaccctt acaaactgga ttaaaagcaa ttgatgcttt ggttccaatt ggtcgtgggc
     9601 aacgtgagtt gattatcgga gaccgtcaaa caggaaaaac atccgtagct attgatgcga
     9661 ttttgaacca aaaaggtcaa gacatgattt gtatctatgt ggctattggt caaaaagaat
     9721 caaccgttcg tacacaagtt gaaacccttc gccgttacgg tgccttggat tatactattg
     9781 ttgtgacagc gtcagcctca caaccatcac cattgctctt tatcgctcct tatgcgggtg
     9841 ttgccatggc tgaagaattt atgtaccaag gcaagcatgt cttgattgtc tatgatgact
     9901 tatcaaaaca agcggtggct tatcgtgaat tgtctctctt gcttcgtcgt ccaccaggtc
     9961 gtgaggctta cccaggggat gttttctact tgcatagccg tcttttggaa cgctctgcaa
    10021 aagtttctga tgatttaggt ggaggttcta tcacagctct accatttatc gaaacacagg
    10081 ccggagatat ctctgcatat attgcgacta acgttatttc gattacagac ggacaaatct
    10141 tcttgcaaga gaacctcttt aattcaggaa ttcgtccagc gattgatgca ggatcatctg
    10201 tctcacgtgt aggtggttca gctcaaatta aagcgatgaa aaaggttgct ggtactcttc
    10261 gtttggattt ggcttcctat cgtgaattag aagcctttac ccaatttggt tcggaccttg
    10321 atgctgcaac acaagcaaaa cttaatcgtg gtcgccgtac ggttgaaatc ttgaagcaac
    10381 cattgcataa accgttacct gttgaaaagc aagtcgttat tctttatgcc cttactcatg
    10441 gtttcttaga tgatgttcct gtagatgata ttttggcttt tgaagaagct ctttacgact
    10501 actttgacgt tcattacaat gatttatttg aaactatccg taccacaaaa gaccttcctg
    10561 aagaagctgc ccttgatgcc gctattaaag cgtttaaaga acactcaaac tttaagtcat
    10621 agagggagga tactagtatg gcaggctctc taagtgaaat taaagctaaa attatttcaa
    10681 ctgagaaaac aagtaaaatc actagtgcca tgcgtatggt ttcttctgca aaacttgtta
    10741 aatctgagca agcagctcgt gattttcaaa tttacgcctc aaaaattcgc cagattacta
    10801 cagatttatt gaaatcagaa ttaaccatag gatctgataa tccgatgttg gtgtctcgtc
    10861 ctgtcaaaaa aacaggctat attgtcatca cgtctgataa aggacttgtt ggaggttaca
    10921 attcaaaaat cttgaaatct gttatggata tgattacaga gtaccatgct gatggtgact
    10981 atgaaatcat ttctattggt agtgttggtt cagacttctt caaagcaaga gggatgaatg
    11041 tggcttttga acttcgtgga cttgcggatc aaccaagttt tgaacaagtg agacaaatca
    11101 tttcacaatc cgttgacatg tttgtcaatg agatttttga tgagttatat gtctgttaca
    11161 atcaccatgt gaatagctta accagccaag tccgtgttca acagatgttg ccaatttctg
    11221 acttggttgc tgatgaagca gcagaagaag gtgttactgg gtttgaatta gaacctaatc
    11281 gtcatgatat tttagatcaa ttgttacctc aattcacaga aagtttaatt tatggggcta
    11341 tcattgatgc taaaacggct gaacatgcag caggtatgac tgccatgcag acagcgacag
    11401 ataacgctaa gaatgtcatt aacgatttga ccatccaata taaccgagct cgtcaagcag
    11461 caattacaca agaaattact gaaattgttg caggagcaaa tgcactagaa taaaaaataa
    11521 cgctgactag cgattaggtt attgtcagca ttcaatttta tcaatcgaga taaaatgact
    11581 tatgggactt ttctattgaa agactgctct tttcagtata aaagaagtta gcaagactaa
    11641 ctaagccctg actatcgaat ataaggagaa atgaatgagc tcaggcaaaa ttgctcaggt
    11701 tgttggtcca gtagtagacg ttatgtttgc gagtggggac aaacttccag agattaataa
    11761 tgcattgatt gtttataaag atagtgataa aaagcaaaaa atcgtccttg aagttgctct
    11821 ggaacttggt gacggtatgg tgcgaacaat cgctatggaa tcaactgatg ggcttacacg
    11881 tgggttagaa gttcttgaca ctggtcgtgc gattagcgta ccagtaggta aagaaacttt
    11941 gggacgcgtc tttaatgtac ttggagaaac cattgatttg gaagaaccat ttgcagaaga
    12001 tgttgaccgt cagccgatcc ataaaaaagc accatcgttt gatgaattat caacatcatc
    12061 agaaattctt gaaactggta tcaaggtaat tgaccttctt gccccttacc ttaaaggtgg
    12121 taaagttgga cttttcggtg gtgccggagt tggtaagact gtccttatcc aagaattaat
    12181 ccacaatatc gcccaagaac acggaggtat ttcagtattt accggtgttg gtgagcgaac
    12241 acgtgaagga aatgaccttt actgggaaat gaaagaatca ggcgttattg aaaaaaccgc
    12301 catggttttt ggtcagatga atgagccgcc tggggcacgt atgcgtgtag cccttactgg
    12361 tttaaccatt gctgagtatt tccgtgatgt agaaggccaa gatgttttgc tctttattga
    12421 taatatcttc cgtttcactc aggcaggttc agaagtatca gccctcttag gccgtatgcc
    12481 ttctgctgtt ggttaccaac caacacttgc tactgaaatg gggcaattgc aagaacggat
    12541 tacatcaact caaaaaggtt ctgttacatc tatccaagcg atttatgtac cagcagatga
    12601 ttatactgac cctgcgccag caacagcatt tgctcacctg gattcaacaa ctaaccttga
    12661 acgtaaattg acacaaatgg ggatttatcc agccgttgat ccattggctt caagttcacg
    12721 ggctttatca cctgagattg ttggtgaaga gcactatgct gtggcaacag aagttcaacg
    12781 tgtgcttcaa cgctaccgtg aattacaaga tattattgct attttgggga tggatgaatt
    12841 gtcagatgag gaaaaaacat tggttggacg tgcgcgtcgt atccagttct tcctctcaca
    12901 aaattttaac gttgctgaac aatttaccgg tcttcctggt tcttacgtgc cagttgctga
    12961 gacagttcgc ggctttaaag aaattcttga aggaaaatac gatgatcttc cagaagacgc
    13021 ctttcgttca gttggtccaa tcgaggatgt gattaaaaaa gccgaaaaaa tgggctttta
    13081 gtgaggtgat gccatgacac aaatgactgt tcaagtggta acgccagatg gaatcaaata
    13141 tgatcaccat gctaaatgta tttcggtaac aacaccagat ggggaaatgg ggattttacc
    13201 taatcacatc aatttaattg cacctttgca agtccatgaa atgaaaattc gccgaggtgg
    13261 tgaagatgaa aaagtggact ggattgctat caatggtggt attattgaaa tcaaagataa
    13321 tgtggttacc gttgtcgctg attctgcaga acgtgaccgt gatatcgatg tcagccgagc
    13381 agagcgagca aaactaagag cagaacgtga aattgcacag gctgaaacga cccacaacat
    13441 tgatgaagtt cgtcgtgcca aggttgcctt gcgccgcgct ttgaatcgta tcaacgttag
    13501 taaaaagtaa gtggtgttga taaaagacaa ggataagtaa gaaaggtttt taacctatta
    13561 ttttgtttgc tgagtctttt taacatcagt ttctgagagt ttgaccatac taccttctct
    13621 aaacacgtta ttattgttga gaggagttat atgggggaac tcttttttgt atccacgttg
    13681 ttagtagaaa ctcagtaatg acgcttaatg tatttttagg ctatcaactt agtcagagag
    13741 gatgtcaatt taattgataa gttggacgga tcaagatggt atgttataat aatcctatgg
    13801 aattattaat gaagatcatt gccttttcgt gtcatttatt ttttattggc cttagttacc
    13861 aattattgat tagtgtgatt gactggacaa aatttagtca ttatcatcca gaaaatcttg
    13921 gccggctacg actttttgtc tttttattag ctactgccct agggtatttg gtgagccatt
    13981 gtatgctgga attgcttcaa attagccaga cgctcttttt tgagttctgc taatactgga
    14041 ataaaagaag tatggtataa ttttactacg actaagagat agataaggga gaaaattgtg
    14101 gataaaataa tcattgaagg tggtcaaaca cgcttagaag gagaagttgt tattgagggt
    14161 gctaaaaacg cagttcttcc tttactggca gcatctattt taccttcaaa aggaaaaaca
    14221 attcttagaa acgttcctat tctatctgat gtttttacca tgaataacgt ggtacgtggt
    14281 ttagatattc gtgttgattt taatgaagca gctaacgaaa tcacagttga tgcctcagga
    14341 cacattttag atgaagcccc ttacgagtac gtgagccaaa tgcgtgcctc tattgttgtt
    14401 ttaggaccaa ttttggcacg taatggccac gcaaaagtat caatgcctgg aggctgtacg
    14461 attggcagta ggcctattaa tttgcactta aaaggattag aggctatggg ggcaacgatt
    14521 actcaaaaag gtggagatat tgccgctcaa gcagatcgac tgcaaggtgc catgatttat
    14581 atggacttcc catctgtagg agctacccaa aacttgatga tggctgccac tttagctgat
    14641 ggtgtgacca ctattgaaaa tgctgctcgt gagcctgaaa ttgttgattt agctcaattt
    14701 ttgaataaaa tgggagctag aattcgtggt gctggaacgg agacgttaac gataacaggc
    14761 gtcacaagtc ttcatggggt ggagcacgat gtggttcaag accgtattga agcaggtact
    14821 tttatggttg cagcggcgat gacttcaggc aatgttttga ttagagatgc tgtttgggaa
    14881 cataatcgtc cattgatttc aaaattaatg gaaatgggtg tttctgtaac agaagaagaa
    14941 tatggtattc gcgttcaagc aaacacgcca aagctcaaac ctgtaacggt taaaaccttg
    15001 cctcatccag gttttccaac agacatgcaa gctcaattta cagctttaat ggcagtggtc
    15061 aatggagagt ctactatggt tgaaactgtc tttgaaaatc gatttcaaca tctggaagaa
    15121 atgcgacgca tgggcttaca atctgaaatc cttagagaaa ctgccatgat tcatggtgga
    15181 agacaactgc aaggtgctcc tgtgatgtca acagatttac gtgccagtgc tgcccttatc
    15241 ttgacaggga ttgttgctca aggagttaca atagttaata atttggttca tttagatcgt
    15301 ggctactatc aatttcatga aaaattagca aagcttggag ctactattag tcgtagcagt
    15361 gaggtctagc ttatgacaaa tggtttaaaa tatgtcttag aacaaatgtt attactttta
    15421 gtaattgctg ccttggcgtg tcttttttta gccattggac ttatgattgg ttatagtttt
    15481 atgggagatg gccatagtcc atggcatatt ttatctatgg ataagtgggc agagttagtg
    15541 aataaattta ctggaaagta ggccttgcct actttttata gcatctgaga ggaagtatgt
    15601 taacaaggaa aaaacgaaaa agggtgcaag agcaaaaggt tcctaaaaga gctaatgcct
    15661 tacttactaa gtcaacccgg caatatcaag accgttcaca acttaagaaa ggttaccgtt
    15721 catttaagcc agcaggttgg caccgattgc accacttaaa aggaagttat gatcacgcgg
    15781 ttgatagagg gcatttgcta ggttatgctt tagttggtgg actgaaaggt tttgatgctt
    15841 ctactggtaa tcctgataat attgccactc aactaagttg ggctaatcaa gcaaataaac
    15901 cctactcgac aggtcagaat tactatgaag gtttagtacg tcgtgctttg gataaggggc
    15961 atcgcgtccg ctaccgtgtc accctacttt atgatggtga taatctacta gccagcggta
    16021 gccatttgaa ggctaaatcc tctgatgaca gcttggcttt taacgtgttt gttccaaatg
    16081 tccaagcagg attgaccgtt gactatcgga cgggacaaat agctatcaat ctctaagcga
    16141 aaagtcactg atttaagatt gtcactaagc actttttttg atagaataga actaattgaa
    16201 taagtgtcta tgagactagt actaatagag aaattaacag ggagtgaggg ccgtgactgg
    16261 aagccttctt aagggaacta ttaggaattc actcagctaa agacaattaa ttaaggtctg
    16321 ataacatcag ataaaaacgg atggtaccgc gtgtcaacgc tccgcttatc aagtggagga
    16381 gtggcatgct tttttcatcg ccaaaagaag ggaaaactat ggatttacaa gcacaattag
    16441 aggaattaaa gtcaaaaaca ttggagacct tacagtcttt gacgggtaat cataccaaag
    16501 aactgcaaga tttacgcgtg gctgttcttg ggaaaaaggg atctttgaca gaattactaa
    16561 aggggctaaa agacttgtct aatgacttgc gtcctgtggt tggtaaacag gtgaacgaag
    16621 ttcgtgactt attgacaaaa gcctttgaag aacaggccaa aattgtagaa gctgctaaaa
    16681 ttcaggccca acttgatgct gaaagcattg atgtgacttt accgggtcgt caaatgactc
    16741 tggggcatcg tcatgtgttg acacaaacca gtgaagaaat cgaagatatt ttcttaggaa
    16801 tgggttttca gattgttgat ggctttgagg tggaaaaaga ttattacaac tttgagcgca
    16861 tgaatttgcc aaaggatcac cctgcccgtg acatgcaaga tacattttat atcacagaag
    16921 aaatattgtt acgaactcat accagtcctg tgcaggcccg tactcttgat caacatgatt
    16981 tttcaaaagg gcctttaaaa atggtttctc caggtcgtgt tttccgtcgt gatactgatg
    17041 acgctaccca ctcacatcaa ttccatcaaa ttgaaggttt ggttgtgggc aaaaatattt
    17101 ccatgggaga cctcaaaggg actcttgaaa tgatcatcaa gaagatgttc ggtgaagaac
    17161 gtagcattcg tttgcgcccg tcatatttcc cgttcacaga accatctgtt gaagtggatg
    17221 tttcttgctt taaatgtggt gggaaaggct gtaatgtctg caagaaaaca ggatggattg
    17281 aaattcttgg tgctggtatg gttcacccaa gtgtcttgga aatgtcaggt gttgatgcaa
    17341 aagagtattc aggctttgcc tttggtttag gtcaagagcg aattgccatg ttgcgttatg
    17401 gcattaatga cattcgtggt ttctatcaag gcgaccaacg tttctctgaa caatttaact
    17461 aaaacgagtc gtttgaagac ctctgtcagc tattttatct aagattgagt aaaagttgat
    17521 aggtaatgat aataggttaa ttgtctaagg agaaagtccc tctcgcttca acgtcataag
    17581 ggttttgact tggaacttaa aatacgaaag gaaatgactc aaatcacggc ttgagagctt
    17641 tctctagccg agtgatttgg gcactaaaat tatgttagta tcttataaat ggttaaaaga
    17701 attagtagat atcgatgtga caccagtagc acttgctgag aaaatgtcga caacgggtat
    17761 cgaggtagaa ggaattgaag tccctgcaga aggtttatca aaactggttg tggggcatgt
    17821 gctttcttgt gaagatgtgc cagagacaca tttgcatttg tgtcaggtag atacaggtga
    17881 tgaaacgcca cgtcaaattg tgtgtggcgc accaaatgtg aaagcaggga ttaaggttat
    17941 cgtggctgtg ccaggtgctc gtattgcgga taactataaa atcaaaaaag gcaaaatccg
    18001 cggcatggaa tctcttggca tgatttgttc cttgcaagaa cttggtttgt cagatagtat
    18061 cattccaaaa gaattttcag atggtattca aatcttgcca gaggaggctg tgccaggtga
    18121 tgctattttt aaatacctgg acttggacga tcatatcatt gaattgtcca ttacacctaa
    18181 ccgtgctgat gcgctttcta tgcgaggtgt ggcacatgaa gtcgcagcta tttatggcaa
    18241 gtcagtatca ttcccccaaa aaaatctaca agaatctgat aaggcaactt cagaagccat
    18301 tgaattgtcc attgcctctg ataacgttct aacttacgct agccgtgtcg ttgaaaatgt
    18361 caaagttaag ccaagtccac aatggttgca aaacctttta atgaatgctg gtattcgtcc
    18421 tattaataat gtggttgatg tgaccaacta tgtactcttg tatcttggtc aacctatgca
    18481 tgcttttgat tatgataaat ttgaagacca taagattgtg gcgcgtgcgg ctcgtcaagg
    18541 agaaagtttg gtcacacttg atggtgaaaa acgggatttg accactgagg atcttgttat
    18601 taccgtggct gagaaaccag ttgctttagc aggtgttatg ggtggtcaag ccactgaaat
    18661 tgatggcaac tctcagacgg ttgttcttga agctgctgtt tttgatggca aatcgattcg
    18721 taaaaccagt ggtcgtttaa accttcgttc ggaatcctct tcacgttttg aaaaaggggt
    18781 taactatgca actgttttag aagcgcttga ctttgcagcg gctatgttac aagaattagc
    18841 agaaggtcaa gtcttatcag gccatgttca agctggacaa ttgccaacag aacctgttga
    18901 agtctcaact agccttgact atgtgaatgt tcgtttagga acagaattaa cttttgctga
    18961 tattcaaagg atttttgacc aacttggatt tggtctaaca ggagatgaaa caagcttcac
    19021 ggtagctgtt cctcggcgtc gttgggatgt tagcatccca gctgacttgg tggaagaaat
    19081 tgcgcgtatc tatggttatg ataagttgcc gacaacctta ccagaggcag ggggaacggc
    19141 tgcagaatta acgccaacac aggctcttcg tcgtaaagtc cgtggcttag cggaaggatt
    19201 gggcttgact gaaatcattt cgtatgcctt gacaacacca gagaaagctg ttgagtttgc
    19261 agtggcacca agtcacctaa cagaactcat gtggccaatg tctgtggaac gttcagcgct
    19321 tcgtcaaaat atggtttcag ggatgttaga tacggtagct tataatgtgg ctcgtaaaca
    19381 aagtaacttg gctctttatg aaattggaaa aatctttgaa caagaagcta atccaaaaga
    19441 agaccttcct aatgaagtga accattttgc ttttgctatt tgtggcttag tggcacaaaa
    19501 agatttccaa acccaagctc aggcagtgga tttttaccat gctaaaggta tcttggatac
    19561 tttatttgca aaccttaacc tcaaggttca atatgtgcca accaaagacc tagcaaacat
    19621 gcacccagga cggacagcgc ttatcctatt agatgagcaa gtgattggat ttgttggtca
    19681 agttcatcca ggaacagcaa aggcttatag tattccagaa acctatgtgg cagaattgga
    19741 tatggctgct ttagaagcag cgttgccaag tgatcaaact tttgcggaaa tcacaaaatt
    19801 ccctgctatg acacgtgatg ttgccttgtt acttgaccgt gaggtgagtc atcaggccat
    19861 tgtaacagct attgaatcag caggtgtaaa acgcttgact agcattaaac tctttgacgt
    19921 ctatgaagga gcaaccattc aggctggcaa aaaatcaatg gcttatagcc ttactttcca
    19981 aaatccaaat gacaatttaa cggatgaaga agttgccaaa tacatggaga aaatcaccaa
    20041 agcattgaca gaacaagtcg gtgctgaagt acgctaagaa aaaaactggg cctctaaggc
    20101 tccgtttttt tctgttataa tagaggaaaa aagaactcca gttcaaatga ttggagaaaa
    20161 acatcagaag gtggaggaaa atcagaatgt atcaaacaaa aataacaggc aaccgccttt
    20221 accacacggt ttcgaaaggc tacggagata gtgtaacctt atttggaaaa actgatcaag
    20281 gggaaacccc tatgagttta ctggttattg ccttgtcttc ttgtgtgact atgtgtgtgc
    20341 agggttttta ccatcgtcaa tttcagcaag acacagttgc tgtttctgtt gaggctagtt
    20401 atgaggaaga agtttttgaa ctacttgtta ggattgatga tgttttagat gacaaaaaga
    20461 aagctgattt attggcttac attggaactt attgtcgtgt gaaacaactc ctcaaacctg
    20521 aagttacggt gaccattacg ttggaaggat tgtctcatgt ctgatgctag tttgaaaact
    20581 taccaaaaac aatgggctta tcaaaagtat tgggtcatgg cacactcaca gcagcattac
    20641 aatgctttaa gagaattgtt taaaggaaat cagtggtcag aagaaaaggt gttgactttt
    20701 cattgtttga ttgaagaagc gcaagccatt cctcccacag taaaaagctt acgaacggca
    20761 tatcagcatg tttgggggta ttttaaaaag gtagcgagtc aggaagaaaa agaccatttc
    20821 aaagatttgg atgctcagtt ggaaactaaa tctgaggaga tgctatgctt tttacaagag
    20881 atgactgctc attatcagcc ctcttatctg ctatcttgcc gtttgattac caaaggtcct
    20941 taaatgctaa acagaaaagt agggatttct gggaagttat gctaagctag tgatataaat
    21001 aagggggaat gagagatgtt tttagcgctt aatgaaatga aacaatccaa attgcggtat
    21061 ggtttgattg ctggtttgct atgtttggtt gcctatttga tgttcttttt gtcaggattg
    21121 gcctttggtt tgatgcagga gaaccgctcg gcggttgatc tttggaaagc tgacagtgtt
    21181 ttactagcca aggatgctga cgctaccttg accttatcac aggtgtccag agctcaagaa
    21241 aatcaaataa cagcagacaa ggtagctcct cttgctcaac tcaataccgt ggcgtggtca
    21301 gttaaaaatc ctaaggatgc cgacaaagtt aaggttagac ttttcgggat tgattctaat
    21361 agctttattc gtcctaacat tgtaaaaggt cgattattta agactaacaa agaggttgtt
    21421 ttggatcaaa gccttgcaaa agaggaagct tttgcgattg gcaaggactt ttacacatcg
    21481 agttctagtc aagcattaac tatcgttggt tatactcaaa atgctagatt tagtgttgca
    21541 ccagtggttt atatgaattt ggaagctttt gaaacattaa aatatggaga accactacca
    21601 aaagataagc aagttgttaa tgcttttatc actaaaggaa gtttaacaga ttatcctaaa
    21661 aaagacttcc aaaaattaga tattaaaacc tttattacta aattacctgg ttatagcgct
    21721 caacttttaa cttttggctt tatgattagt tttcttgtca ttatttcagc tattattatt
    21781 ggtattttta tgtatatttt gactattcaa aaggcaccta tttttgggat tatgaaagcg
    21841 caaggtatcg ggaatataac gattgcacaa gcagttctgc tgcaaacctt cttgttaagt
    21901 gctttaggaa gcggtttagg gttattggga acttggttga cgtctttagt gctccctgaa
    21961 gcagttccct tccaaagtaa ttggctagtt tacttagcga tattagtcag tatggtttgt
    22021 tttgctttgt taggaacctt attttctgtt tttagcattg tacgaattga tcctttgaaa
    22081 gcaattggat aggaggtagt gatgtcggtt ttaacattta aacaagtgac caaaacattt
    22141 caagatggtc atcacgaaat caacgcatta aaggcaactg acttttccat tgaagctgga
    22201 gaatttgtgg ctattattgg accatcaggt tctggaaaat caaccttttt gaccattgct
    22261 ggtggcttac agaccccaag ctctggtcag ctaatcattg atggcactga ttatacccat
    22321 ttatcagaaa aagagcgctc acgcttgcgc ttcaaatccg ttggttttat cttacaagct
    22381 tccaatctga tacctttttt aacggtccag cagcagttgg aattggtcga tcatttgaca
    22441 ggaagcaaag aaaaagccaa agccaatcag ttatttgacg atttaggcat tgcagggcta
    22501 aaacatcagt tacctcaaga attgtccggc ggagaaaggc aaagagcggc tattgcccgt
    22561 gctctttacc atgatccagc actgatattg gcagacgaac ctactgcaag tttggatact
    22621 gaaaaagcct atgaagtggt caaactgttg gctaaggaaa gtaaagaaaa aaacaaagcc
    22681 attattatgg tcactcatga tgatcggatg ctaaaatatt gtgacaaagt ctatcggatg
    22741 caagatggag agttacgtca agagagatag tagactggta agagtttatg atattagaag
    22801 aaaaaaccaa acaagcacag cacttgtctg gtttttttct aagtgtgact attatcaata
    22861 cgtcaccatt tccctaaagc ttactatacg ggatgctaaa ggtatcacca tgctcaaagt
    22921 cgccatattg gtaacctcgg tcaaaccaac gttggcgttg cttagaagtt ccgtgggtaa
    22981 aactatcagg aacggctctg ccataggttt cttcttgcag agtatcatcc ccaactgcgt
    23041 gagctgctgc cattgcttcc tcaatatctc ctttttccaa aaggccttgc ccttgaacat
    23101 aattagccca ggcaccagca tagtaatcag cttgtaattc caacttgacg ttgagctgat
    23161 tggctttagc ctttgatttt ccttgtctag tgctagcgta attatccata attcccaatt
    23221 cattttggat gtggtggcca acttcatgag caatcacata ggccatagcg aaatcacctt
    23281 ttgcgccgta cttggttgat agttcattat aaaaagaaat atctaaatac actttttgat
    23341 cgcctgggca atagaaagga ccagaagagg cttgccccct gccacaagcg gtttgagttg
    23401 ctccagtata tagaactaat gttggtttat gataagttaa ccctttctca cgaaatgtct
    23461 tagtccaata gtcctcggta gaggcgaaga ccttactgac aaaagaaacc tgttcttgac
    23521 tagccttgtc accatttgta cgcgttactt ggcttgattg ataggcattg ttgttggtag
    23581 aagaaggctt gcctcctaaa acaccagata ggccacctcc tcccataact aggagaagga
    23641 gaagaataac cagcttggtt ttccaacctc ctcgtgaaaa gagtaattga agcaggagac
    23701 cgccccctag gccgccgccc ccaaaagaac cagacgattg accacgtcta tcttcaacct
    23761 gttgactttc tctcaaatca tccgttttca taagtttctc ccatctattt cttgatttta
    23821 ttataaaata attctattag aattgctatt tttatgatga gattataact aaaaatagga
    23881 aaagcaatag gtatttgcta taatataaca agggtagatt cagtttgtat aaaggagtca
    23941 tatgaaatta atttacacag agatgagtta ctcgatgaca gagattttgg tgaatgaagc
    24001 acgaaaagct gctgaccaag gttatcgagt gttctatatt gctccaaatt ctctttcatt
    24061 tgaaaaggaa agagaagtct taaccttatt gcctgaacga gggacttttt cgataatagt
    24121 cacccggttc gttcaaatgt caaggtattt tacagttgag agctctcctt ctaagcaaca
    24181 tctagatgac acgactttgg ctatgatttt ttatcgggcc ttgatgcaat taaaaccaga
    24241 agatttgccg agttatggtc gtttgcaaaa taattcggtt tttattgaac aactagttga
    24301 gttatataag gaattaaaaa acgcacaatt aagcgtacat gatttaacag ggttagacca
    24361 tcctcaaaaa caagaagatc ttattaaaat tattgagctt gcagagacga tcatgattca
    24421 gcaagattac aatcaagata gtcctctaca atcttttgca agagcaatta agttaggact
    24481 tttaaataat caactatcaa aaacagttat tgttattgat ggtttttcac gtttttcagc
    24541 tgaagaggat tatttattat cacttttaaa taacaactgt caagaggtca tcattggcag
    24601 ttatgtgagt caaaaagctt atcaaaaatc ttttattaag ggaaatatct atgaggctag
    24661 ccttcatttt ttgcaagatt tggctcaaaa ataccatatc aagccggtat ttgcgacctc
    24721 taatcaagtg tttaaaccag ccttttcacg gctaacacaa ttatttgaag caactcatga
    24781 tttttcacaa gtagactggc aactccaaaa gaatgatctt gatcacttta gcctttggca
    24841 atgtcatcat caaaaagagg aaattgaaca tgtagcaaaa tccattcggc aaaagctata
    24901 tgaaggttac cgttataagg atatcttagt gttgctagga gatatggatg cttatcaatt
    24961 acaaattggg cctatctttg ataagtttga aatcccctac tatttaggaa aagcagagcc
    25021 tatggctgcc catccattag tccaatttat agaatcgctc gaacgaagtc agcgctataa
    25081 ttggcgtcgt gaagacattt taaacatgtt aaaatcagga ctttttggtt gttttgatga
    25141 tagtgatatt gatcgctttg aggaatatac tcagtttgca gatattaaag gatttactaa
    25201 attttcaaag ccttttacca ttaactcttc taggcagtat cctcttgact ttttaaatga
    25261 aatgagacaa gacatcgttt tacctttgca agaattgttt aagagtcaaa aacagcttgg
    25321 tgcttcactt attgacaaat taatactttt tttagaaaag attagattag ctgagaatat
    25381 gcaaggttta gcacagtccc aattagaggt ggaaaaaaac gaagaagttt ggaaaaggtt
    25441 tacagatatt ttgacttctt ttcatcatat ttttggccaa gaaaaattac ggctatcaga
    25501 ttgccttgct ttaatcaaaa cgggaatgaa atcagctcag tatcgtgttg ttccagcgac
    25561 attagatgtt gtcactatta aatcttatga ccttgttcaa ccacattcaa aacctttcgt
    25621 ttatgcaatt ggtctaactc aatcacattt tcctaagcaa attcaccatt caggactact
    25681 ttctgatcaa gagcgtgcta gaattaatga aataagaaat tatcgtcatt ttgatattgc
    25741 tagtgccgaa aatagtaaaa aaaaccatca aactgcgtta tctctgttta atgctgctac
    25801 taaagagtta gttcttagtg ttccaacggt aatcaacgag acattcgatg atctatctcc
    25861 ttatcttaaa gaattaataa actttgggtt acctcttttg gataaaggca aaaactatct
    25921 atcttatgat aatagcgata ttggtaacta taaagctctc ttgtcacaaa ttattgctat
    25981 taaccgacaa gatcttatag aaatgtcaga tcaagataag atgttttgga ctgttgtctt
    26041 acgttatctc aggaaacagt taagaaagca acagctagag ctacctactt cagattatcg
    26101 tttatcaaca aagcctttgt ctaaagaagt tatcgaggta tgttttccaa agggaatccc
    26161 tttaaagcta tctgcaactg ctttaaccgt tttttataat aatcagtaca actatttttt
    26221 gaaatatgtt ttgaatttaa acaaaacaga atccattcat ccagattcca gaattcatgg
    26281 tcaatacttg catcgtgtct ttgagcggtt aatgaaagat cacacgcaag aaccgtttga
    26341 taataagtta aaacaagcaa tctaccatac caatcaagaa agtttttttc agcaggttta
    26401 ccaagataat gccgaggctg agtacagttt agctatacta gaagatattg ttagatcaac
    26461 agcacctatc ttgcaactta accaaaacat aaaggtcatt gatcaagaaa agaactttca
    26521 tctggatatg ggaaatgaga tattagtcca cgggatcatt gatcgtattg accagctgag
    26581 cgacggtagc ttgggcattg ttgactataa atcaagcgcg aatcaatttg atattgggac
    26641 cttttataat gggttgagtc cacaactagt gacttacctc gcagccttaa aacagatcgc
    26701 accgcacgat ataaaccaat tatttggggc tatgtacctt catttgcaag atcctaaatt
    26761 ggatctagta acatttaaac aaattgataa cacattagtg gagagtatct ataaagcgtt
    26821 gacctataaa ggtatttttt cagaagtcga aaaagagcat ctatctactg gtgcttacca
    26881 aaccaaaaat gctctttatt ccaatgacga attggaaaca cttttaaatt ataacaagta
    26941 cctctatctt aaagctgcta aacacattaa aaagggacat tttttgatta atccttatac
    27001 aagcgatggt aagacagttc aaggagatca attaaaagcc attacccgtt ttgaagcaga
    27061 tcttgacatg gcacaggcaa ggcgactagt gactctccct gctaaggaga aaaaagagtg
    27121 ctttttaaca ttaatgagaa aggagagcca cttgtgattt cttttgcccc atttttaagc
    27181 cccgaagcta ttaaacattt acaagaaaac gaaaggtgca gtgatcagtc tcaaaaacgc
    27241 acagctcaac aaattgaagc aatttatact agtggccaaa atatacttgt atcagcttct
    27301 gctggttcag gaaaaacctt tgtaatggtc gaacgcatac ttgataaaat tttgagaggt
    27361 gtttcaattg atcggctttt tatctcaacc tttactgtta aagcagctac agaactgcgt
    27421 gaacggattg aaaacaaatt atactcacaa attgctcaaa ctacagattt tcaaatgaaa
    27481 gtttatttaa cagaacaatt gcaatctctt ggtcaagctg atattggtac tatggatgct
    27541 tttgcacaga aagtagtaag tcgctatggt tatagcattg gcatttcatc ccaatttcgt
    27601 atcatgcagg ataaagcaga acaagatgtt ttaaagcaag aggtgtttag caaactcttt
    27661 agtgagttta tgaatcaaaa agaggcaccg gtgtttaggg ctcttgtgaa aaatttttct
    27721 ggtaactgta aagacacttc agcttttaga gagttagttt atacttgtta ttcttttagc
    27781 caatcgacag aaaatccaaa aatatggttg caagaaaatt ttctaagcgc tgctaaaact
    27841 taccaaagac ttgaagatat cccggatcat gatattgaac tcttactttt ggcaatgcaa
    27901 gacactgcaa atcagctaag agatgtgact gatatggaag attatgggca gctgactaag
    27961 gcaggtagcc gatctgctaa atacactaaa cacttaacga tcatagaaaa gttgtctgat
    28021 tgggtgcgtg attttaaatg tttgtatgga aaagccggat tggatcggtt gatcagagat
    28081 gtgacaggcc ttataccatc tgggaatgat gttacagtct cgaaggtaaa ataccctgtt
    28141 tttaagacct tgcatcaaaa attaaaacaa tttaggcatt tagaaacaat tttaatgtat
    28201 cagaaagact gttttccctt attggaacag ttacaagatt ttgtgcttgc gttttcagaa
    28261 gcttatttag ctgtcaaaat acaagaaagt gcttttgaat tttcagatat tacacacttt
    28321 gcaatcaaaa ttttagagga aaatacggat attcgccaat cctatcagca acactatcat
    28381 gaggtgatgg ttgatgaata tcaagataac aatcatatgc aagagcgact cctgacctta
    28441 ctatcgaacg gtcataatcg ctttatggta ggagatatca aacaatcgat ctatcgattt
    28501 cggcaagccg atcctcagat ttttaatcaa aagtttagag actatcaaaa aaaaactgag
    28561 caggggaaag tgattttact caaagaaaac tttcgtagcc aatcagaggt gttaaatgtc
    28621 agcaatgctg tttttagtca cttaatggac gaatcagtag gagacgtctt atacgatgag
    28681 caacatcagt taatagcagg tagtcatgct caaacagtcc cctatctaga ccgtcgtgct
    28741 cagttattgc tatataatag cgataaagat gatggcaacg ccccttcaga tagtgagggt
    28801 atttcattta gtgaggttac aattgttgcc aaagaaatta ttaagcttca caatgataag
    28861 ggtgtccctt ttgaagacat tacgttactc gtttcttcaa gaacaagaaa tgatatcatt
    28921 tctcatacat tcaatcaata tggtattcct atagtaacag atggtgggca gcaaaactat
    28981 cttaaatctg ttgaagtgat ggttatgtta gatacattac gcaccattaa taacccaaga
    29041 aatgattatg cccttgtggc tttactgcgc tcaccgatgt ttgcctttga tgaggatgat
    29101 ttagcaagaa tagcacttca aaaagacaat gagctagata aagattgcct atatgacaag
    29161 atacaaaggg ctgtgattgg aagaggtgct catcctgaat tgattcacga taccttgctt
    29221 ggcaagttaa atattttttt aaagacgttg aaaagttggc gtcgatacgc taagctaggg
    29281 tcgttgtatg acttgatttg gaaaattttt aatgatcgtt tttattttga ttttgtagct
    29341 agtcaagcaa aagcagaaca agcacaagct aatctatacg cattagctct acgtgctaat
    29401 cagtttgaaa aatcgggcta taaagggcta tactgtttta ttaaaatgat tgataaggta
    29461 cttgagacgc aaaatgactt agcagatgtg gaagtggctg ctcctaaaca agctgttaat
    29521 ttaatgacca ttcacaagtc taaaggttta caatttccgt atgtatttat ccttaattgt
    29581 gacaagcgct tctcaatgac agatattcat aaatcattta ttctgaatcg gcagcacggt
    29641 atcggtatca agtaccttgc agatatcaaa ggtttacttg gtgaaacaac actcaattct
    29701 gttaaagtaa gcatggaaac cttaccttat caattgaaca aacaagagtt gcgcttagca
    29761 actttatcag aacaaatgcg cttattgtat gttgctatga cacgagctga aaaaaaagtt
    29821 tattttattg gtaaagctag taagagcaaa agtcaagata tcacagatcc taaaaagtta
    29881 ggcaaacttt tgccgctggc tttacgagaa cagttattga cattccaaga ttggctatta
    29941 gcaatagcag atgtattttc aactgaagat ctttattttg atgttcgctt tattgaagat
    30001 agtgatttga cacaagagtc agtcggacga cttcaaacac cacagttatt aaatccagat
    30061 gatcttaaag ataatcgtca atcagaaaca attgcacggg ctttagatat gttagaagca
    30121 gtgtctcaat tgaatgccaa ttatgaagca gctattcatt tgccaacagt tcgaacgcct
    30181 agccaactta aggcagctta cgagccttta ttagaaccca ttggtgtaga tattatagag
    30241 aaatcttctc gatcgctatc tgattttact ttgccacatt tttcaaaaaa agtaaaagtt
    30301 gaagcaagtc atattggatc agctcttcat cagttgatgc aggtgctccc tttgtcaaaa
    30361 ccgataaatc aacaaacgct tttagacgct ttaagagaaa ttgatagtaa cgaagaggta
    30421 aaaacagctc ttgatctcaa aaaaatagag tcgttctttt gtgatacaag cctaggccaa
    30481 ttttttcaga cttaccaaaa acacttgtat cgagaagcac catttgctat tttaaaagtt
    30541 gaccctatta gtcaagaaga atatgtccta cgtggtatta tagatgccta ctttttgttt
    30601 gatgatcata ttgtattagt ggactataaa acagataaat acaagcagcc cattgagtta
    30661 aaaaagcgtt accaacaaca gttggagtta tatgcagaag ctctcactca aacgtataaa
    30721 cttcctgtga ctaagcgcta tcttgtttta atgggaggtg gaaagccaga aattgtcgaa
    30781 gtttaaggtt gttgtgacac aagggtattt tgcaaatgat aatagtatag ttgaggattt
    30841 tacgaaatgc ttgaaaagct attgaccttt gaataataat acaatataat aagttttatt
    30901 agtgacaaaa aggaataaat atactatgaa ttttaaaaaa ataagatttg gttttttgct
    30961 ttgcctccta ctttatcctt tagcagcttg taacaagtct gaacaactta atcactacga
    31021 aagaattaaa aaaacaagaa aattagtcgt tgctgtaagc cctgattatg ctccttttga
    31081 atttaaagct ttagttaatg gaaaagatac tattgttgga gcagatgttc aattagctca
    31141 ggctattgct gatgaattag acgtcgatct tgagctttca ccaatgagtt ttgataatgt
    31201 tttgtctagt ttacagactg gtaaagctga tcttgctatt tcaggaatct cacatactaa
    31261 agaaagagct aaagtatatg atttttcaat tccttactat caggcagaaa acgctatagt
    31321 gatgagagca tctgatgcta aagtgaccaa aaatattagt gacctaaacg gtaagaaagt
    31381 agccgctcaa aaaggtagta tcgaggaagg tttagttaaa atacaattac caaaggccaa
    31441 tctgatttct ttgactgcta tgggagaagc cattaatgaa ctaaaagcag gacaagtcta
    31501 tgcagttacc ttagaagcac ctgtagctgc tggtttttta gcccaacata aggatctggc
    31561 tttagcaccc tttagcttaa aaaccagtga tggagatgcc aaagcagtgg cccttcctaa
    31621 aaatagtggt gacttaacca aagccgttaa taaggttatt gctaagttag atgagcaaga
    31681 acggtacaag tcatttattg cagagacaat agcactaacg aaaaatacta tgaagtaata
    31741 aggttaataa aaactttatt agtatccg
//
LOCUS       DAUGHY010000019        29378 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.019, whole genome shotgun sequence.
ACCESSION   DAUGHY010000019 DAUGHY010000000
VERSION     DAUGHY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29378
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.019"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            14..685
                     /locus_tag="VH752_000831"
     CDS             14..685
                     /locus_tag="VH752_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ8289193.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
     gene            660..1496
                     /locus_tag="VH752_000832"
     CDS             660..1496
                     /locus_tag="VH752_000832"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEQ8289194.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGET
                     DS"
     gene            1493..2398
                     /locus_tag="VH752_000833"
     CDS             1493..2398
                     /locus_tag="VH752_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ8289195.1"
                     /translation="MRFEFVADKRIKVKTLLKSHDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGTLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSYAIHLTDDFDSVIIGLQKKDRGY"
     gene            2402..3397
                     /gene="pta"
                     /locus_tag="VH752_000834"
     CDS             2402..3397
                     /gene="pta"
                     /locus_tag="VH752_000834"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEQ8289196.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPEGNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKEAFVDIRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIAKGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFVFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            3523..4278
                     /locus_tag="VH752_000835"
     CDS             3523..4278
                     /locus_tag="VH752_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEQ8289197.1"
                     /translation="MGNIALVTGASAGFGRAIVEHLIADGIKVIGAARRLEKLKALET
                     SFGSDNFYPLQMDVTDTKAIDDALTSLPNQWQDITILVNNAGLALGLDKAYEADFENW
                     MTMINTNIVGLIYLTRQLLPHMVSKDAGIIINLGSTAGTIPYPGANIYGASKAFVKQF
                     SLNLRADLAGSHIRVTNIEPGLCEGTEFSKVRFNGDEARVKAIYQGAHAIQPQDIAST
                     VSWVIHQPPHVNVNRIELMPVSQSYGPQPVSRD"
     gene            complement(4472..5161)
                     /locus_tag="VH752_000836"
     CDS             complement(4472..5161)
                     /locus_tag="VH752_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEQ8289198.1"
                     /translation="MKNAISILALLTPENQLEDTFIRELSHHFSHLFEVTILTSKANI
                     QSNQLSTFQGIFSFHEHDIDLPTLYFKTSQHGQGFLVTESVFDQATTVLLLSQYLTRF
                     YQKFDGYFLQYLPLQARLSDAKGNILVDNHAFNGSFLPTTDNEIEDWILAELRLSDSP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLNQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            5587..6315
                     /locus_tag="VH752_000837"
     CDS             5587..6315
                     /locus_tag="VH752_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289199.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNVPQKELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPEM
                     KQWSAEEIRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVVNLFGGDEHA"
     gene            6308..7834
                     /locus_tag="VH752_000838"
     CDS             6308..7834
                     /locus_tag="VH752_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEQ8289200.1"
                     /translation="MPSLFVTFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGIAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIMISFGITLL
                     ALLASYTPMALSQFSKGKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDMYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAKEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKTSLLTKHPELKGILNQLAGKITEKEMQDMNYEVSVKGADANKVARDYLLKTGLIQK
                     "
     gene            8112..9095
                     /gene="guaC"
                     /locus_tag="VH752_000839"
     CDS             8112..9095
                     /gene="guaC"
                     /locus_tag="VH752_000839"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEQ8289201.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAEQLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKAYEY
                     EFVTSLKEDTPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTVEVNGETFKEYYGSASAYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     regulatory      9218..9313
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9400..9981
                     /locus_tag="VH752_000840"
     CDS             9400..9981
                     /locus_tag="VH752_000840"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEQ8289202.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     gene            9981..11264
                     /locus_tag="VH752_000841"
     CDS             9981..11264
                     /locus_tag="VH752_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEQ8289203.1"
                     /translation="MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSA
                     RELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSSAM
                     FGALIASGIYVSLVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
                     QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIH
                     VPTPFYFGMPTFEITSIVMMCIIATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSE
                     GIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVIIGLLPKFGAMAQM
                     IPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFAS
                     LPETAQMFLTNGIVIATLTSVVLNLVLNGKDKQDE"
     gene            complement(11328..12266)
                     /locus_tag="VH752_000842"
     CDS             complement(11328..12266)
                     /locus_tag="VH752_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEQ8289204.1"
                     /translation="MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAINQVAGVKPVSVHSDLFNLIQIGKAHSLSTPSNLNIAIGPLVQAWRIGFE
                     DARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
                     IEDGIDSALINLGGNVRVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTS
                     GIYERQFTSKGKQYHHILDRQTGCPIETDMLSLTIMAPSSFYCDIWATRLFGLDSSMI
                     ITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH"
     gene            complement(12319..12504)
                     /locus_tag="VH752_000843"
     CDS             complement(12319..12504)
                     /locus_tag="VH752_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEQ8289205.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            12642..13211
                     /locus_tag="VH752_000844"
     CDS             12642..13211
                     /locus_tag="VH752_000844"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEQ8289206.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPEI"
     gene            13246..14325
                     /gene="prfA"
                     /locus_tag="VH752_000845"
     CDS             13246..14325
                     /gene="prfA"
                     /locus_tag="VH752_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEQ8289207.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            14325..15164
                     /gene="prmC"
                     /locus_tag="VH752_000846"
     CDS             14325..15164
                     /gene="prmC"
                     /locus_tag="VH752_000846"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEQ8289208.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLTKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYG
                     VSS"
     gene            15148..15738
                     /locus_tag="VH752_000847"
     CDS             15148..15738
                     /locus_tag="VH752_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEQ8289209.1"
                     /translation="MEYLASIIESGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILVFSKEQPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPSHPITTALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGERAVILRQGAITKEELLANVPELRF"
     gene            15756..16208
                     /locus_tag="VH752_000848"
     CDS             15756..16208
                     /locus_tag="VH752_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8289210.1"
                     /translation="MLRPLSKTDCPMLQEINAKALGYLVSLDLLERQYERLIEDCHHY
                     FLAYADKDTNQLLGYVHAERYETLYASDGLNLLGLAVLPAYQRRGIGSALLRALESQA
                     RQEGIAFIRLNSASHRKEAHAFYRNLDYADDKTQLRFIKNLQGDDNDF"
     gene            16198..17454
                     /locus_tag="VH752_000849"
     CDS             16198..17454
                     /locus_tag="VH752_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEQ8289211.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDADTEMLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAIADHVGAYLMVDMAHIAGLVAAGVHPSPV
                     HYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALDPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMGVKESQTIAHLIIKALVN
                     HDQETILEEVRQEVRQLTDAFPLYKK"
     gene            17461..18438
                     /locus_tag="VH752_000850"
     CDS             17461..18438
                     /locus_tag="VH752_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEQ8289212.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFVQFDK
                     DGEPFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALVINLKSGQYYLI
                     EKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQDDFTFQSKMKS
                     TLFKQLEADDVLSPEKLADQLFDDNLTARLTFVDQVKDVIPEPIKISDIDHSRQIKKL
                     ENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIKTK"
     gene            18439..19038
                     /locus_tag="VH752_000851"
     CDS             18439..19038
                     /locus_tag="VH752_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEQ8289213.1"
                     /translation="MFRLLKRACSFLLLFVIYQSFVIHHNVQRVLAYKPMVEKTLAEN
                     DTKANVDLVLAMIYTETKGGEADVMQSSESSSGQKNSITDSQASIEHGVNLLSHNLAL
                     AEEAGVDSWTAVQAYNFGTAYIDYIAKHGGQNTVDLATTYSKTVVAPSLGNTSGQTYF
                     YYHPLALISGGKLYKNGGNIYYSREVHFNLYLIELMSLF"
     gene            19048..20766
                     /locus_tag="VH752_000852"
     CDS             19048..20766
                     /locus_tag="VH752_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289214.1"
                     /translation="MKRLRPYLKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLLSVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHLGYASLVSRLTSDSFQIQTGINQFLRLFLRAPIIVCGAMVMAYWISPSLTLWFV
                     MMVIVLLTLVFVMSHLLGPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KQQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEVPLPLLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSQYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSMPINDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKALKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGE"
     gene            20769..22496
                     /locus_tag="VH752_000853"
     CDS             20769..22496
                     /locus_tag="VH752_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289215.1"
                     /translation="MIKTDHHLLKRVLQDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVVIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQLVFNQFILGLLTILCTIIAMAQID
                     WLMLILVLVLTPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEILRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKAIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEQEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DAGSIKLDKVPIKCYAKEELRSITGMVLQETWLKDATVHELIAYGSEEASRGEVVAAA
                     KAAHAHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLILDEATSSID
                     IRTEAVIQEALKELMRGRTSFIIAHRLSTIQSADLILVMDQGRLVEWGTHASLMSKNG
                     CYARLQKIE"
     gene            22737..24107
                     /locus_tag="VH752_000854"
     CDS             22737..24107
                     /locus_tag="VH752_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEQ8289216.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDAKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIDLFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDVSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(24266..25249)
                     /locus_tag="VH752_000855"
     CDS             complement(24266..25249)
                     /locus_tag="VH752_000855"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEQ8289217.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIINKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            25440..27926
                     /gene="gyrA"
                     /locus_tag="VH752_000856"
     CDS             25440..27926
                     /gene="gyrA"
                     /locus_tag="VH752_000856"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEQ8289218.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTQFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDTLAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELVSTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIDEINTDRENSISKNKDN"
     gene            27946..28695
                     /locus_tag="VH752_000857"
     CDS             27946..28695
                     /locus_tag="VH752_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEQ8289219.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIVVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQMAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLEK
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAPADVLKAFNHSYNQVST"
     gene            28778..29191
                     /locus_tag="VH752_000858"
     CDS             28778..29191
                     /locus_tag="VH752_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ8289220.1"
                     /translation="MKLNAIHHVAIIVSDYHLSKDFYVNKLGFEIIRENYRPDKHDYK
                     LDLSCGRIELEIFGKVTSDPYYQAPPKRVSQPEFKSEACGLRHLAFRVTNIESYVDDL
                     KSLGIPVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
BASE COUNT         9153 a         5075 c         6124 g         9026 t
ORIGIN      
        1 aaggagacaa tagatgacct tagattggga agaattttta gatccttata tccaaacagt
       61 tggtgaatta aaaataaaac tacggggtat tcgcaagcaa tatcgtaagc aaaatcgtta
      121 ttctccgatt gaatttgtta cgggacgtgt taagtcaatt gaaagtatta aagaaaaaat
      181 gattttacgt ggtgttattg aagaaaatat agcacaagat attcaagata ttgctggact
      241 acgtattatg gttcagtttg tcgatgatgt ggaagaagtc ttagctctat tgcgtcaacg
      301 acaagacatg acaattgttt atgaacgaga ttatattcgc aatatgaagt ccagtggcta
      361 caggtcttat catgtggtgg ttgagtaccc tgttgacacc attgaaggtc aaaaaaaggt
      421 cttagcggag attcagattc gtactttggc aatgaacttt tgggcgacca ttgagcattc
      481 tttaaattat aaatatggtg gagacttccc agaagaaatt aaaaagcgtc ttgaagtgac
      541 tgctaaaatt gctttagaat tagacgaaga aatgcgtaag attcgagaag atattcgaga
      601 ggctcaatta ttgtttgacc ccgtgacacg aaatttaagt gatggtgtag gaaatagtga
      661 tgacacagat gaattataca ggtaaggtaa agcgagttgc tattattgca aatggtaagt
      721 accaaagtaa acgcgttgcc tccaaacttt tctccgtatt taaagatgat cctgatttct
      781 atctttcaaa gaaaaatccg gatattgtga tttctattgg cggagatggg atgctcttat
      841 ctgcttttca catgtatgaa aaagaattag ataaggtacg ttttgtagga atccacaccg
      901 gtcatcttgg cttttatacc gattataggg attttgaagt tgataaattg attgataatt
      961 taagaaaaga caagggagaa caaatctctt atccgatttt aaaagttgct attactttag
     1021 atgatggtcg tgtggttaaa gcgcgtgctt tgaatgaagc gacggttaag cgtattgaaa
     1081 aaacgatggt agcagatgtt attattaacc atgtcaaatt tgaaagcttc cgaggtgatg
     1141 ggatttcagt atcgaccccg acagggagca cagcctacaa taaatcttta ggtggtgctg
     1201 tcttgcatcc gacgattgaa gcgctgcaat tgacggaaat ttccagtctt aataaccgtg
     1261 tctttagaac cttgggctca tcaatcatta ttcccaaaaa agataagatt gagttagtgc
     1321 caaaacgatt aggaatttat accatttcca ttgataataa aacctatcag ttaaaaaatg
     1381 tgacgaaggt ggagtatttt atcgacgatg agaaaattca ttttgtttcc tctccgagtc
     1441 atacgagctt ttgggaaagg gtcaaggatg cctttattgg agagactgac tcatgagatt
     1501 tgaatttgta gcagacaaac gaataaaagt aaaaaccctt ttaaaaagtc atgatgtctc
     1561 taaaggacta ttggctaaaa tcaaatataa gggcggtaat atattagtga atggtattga
     1621 acaaaatgcc atttatttat tgcaagtggg agatgttgtc accattgaca ttccaaacga
     1681 agagcccttt gaaaagcttg aagccatccc ttttgatttg gatattgttc acgaggatga
     1741 tcattttttg gtgattaata aacccattgg ctttgccagt attccaagtg ccatccactc
     1801 caataccatt gctaacttta tcaaggcata ttatgtggac aatcattacc tagaccaaca
     1861 agtgcatatc gtgactcgtt tagacagaga tacgagtggc ttgatgttat ttgctaagca
     1921 tggttatgct catgcaaggt tggacaagca actccaaact cgctcaatag agaaacgtta
     1981 ttttgctctg gtttctggaa atgggacgtt gccagatgaa ggagatatta ttgctccaat
     2041 tgggcgttca aaagatagta ttattaccag ggcagttgac cctatgggca aatatgccaa
     2101 gactagctat aaggttgtgg cgcgctattc agaaaatgtg catttagtgg atatcaaatt
     2161 gcatacagga cgtacccatc aaatcagagt tcattttgcc catattggtt ttccactttt
     2221 aggggatgac ctttacggag gtcgtttgga cttaggaata acaaggcaag ctttacattg
     2281 tcattatttg aatttcaaag atccatttac agaaagtgat tgtagctatg caatacactt
     2341 gacagatgac tttgatagcg ttatcatagg tttacagaaa aaagatagag gttattaaag
     2401 aatgagtatt cgtagtttat tcggtggttt aagagaaaaa attcttggta aaaacatgaa
     2461 gattgttttc ccagaaggta atgatgagcg cgtcgtccgt gcagctgctc gtttgaaatt
     2521 tgaaggactc ttagaaccga ttatccttgg acagtcagaa gaggtacgca accttttgac
     2581 aaaattaggt ttcgctgatc aagactatac tatcattaat ccaaatgaat atgcggattt
     2641 tgacaaaatg aaagaagctt ttgttgacat ccgtaaagga aaagcaacgc ttgaagatgc
     2701 tgataaaatg cttcgtgatg tgaattactt tggtgttatg ttggttaaaa tgggattagc
     2761 agatggaatg gtatctggtg ctattcattc aacagctgat actgttcgtc cagcccttca
     2821 aatcattaaa accaaaccag gtatttcaag aacttcaggt gttttcttga tgaatcgcga
     2881 aaatacgagt gagcgttacg tgtttgctga ttgtgctatc aatattgacc caacagcaca
     2941 agaattggca gaaatcgctg ttaacacagc tgaaacagct aaaatctttg atattgatcc
     3001 taaaattgct atgttaagct tctcaacaaa aggttctggt aaagcgcctc aagttgataa
     3061 ggtaagagaa gcaacagaaa ttgctaaagg gttgaaccct gatttggcac ttgatggtga
     3121 gttgcaattt gatgcagcct ttgttcctga aacagcagct atcaaagcac cagattcagc
     3181 agttgctggt caagctaata ctttcgtttt ccctgactta caatctggaa atattggtta
     3241 caaaattgca caacgtttag gaatgtttga tgctattgga ccaatcttac aaggattgaa
     3301 caaacctgtt aatgatttat ctcgtggctc aagcgctgaa gatatttaca aattagctat
     3361 tattacagca gcacaagcta ttgaaagcca agggtaactt aagacccctt gatataggat
     3421 tctaaccgct aagtgatgtg ttagaagttg tcctaagact aattgacatt attaaaaacg
     3481 agttggccag accatctcgt ttttttgaag gtgaggaaat gtatgggaaa tattgcctta
     3541 gtaacagggg cttcagccgg ttttggcaga gccattgtcg aacacttaat tgcagatggg
     3601 ataaaggtta ttggtgccgc tcgtcgtctt gagaaactca aggccttaga aacgagcttt
     3661 gggtcggaca acttttaccc tttgcagatg gatgtcacag ataccaaagc gattgatgat
     3721 gctttgacaa gtctaccaaa tcagtggcaa gatattacta ttttagtgaa taatgcaggc
     3781 ttggcattgg gacttgataa ggcttatgaa gccgattttg agaattggat gacgatgatt
     3841 aataccaata ttgttgggtt gatttatctc acacgtcaat tattaccaca tatggtctcc
     3901 aaagatgctg gtattattat taatttaggg tcaactgcag ggacaattcc ctatccggga
     3961 gctaatattt atggggctag caaggctttt gttaagcagt tctcactcaa tttacgagct
     4021 gatttagcag gtagccatat acgtgtgacc aatattgaac ccggtctatg tgaagggaca
     4081 gaattttcaa aggttcgctt taatggtgat gaggcacggg tgaaggccat ttatcaagga
     4141 gcacatgcca ttcaacctca agatattgcg tcgactgtat catgggtgat tcatcagccc
     4201 cctcatgtta atgttaatcg tattgagctg atgccagtgt cgcaaagtta tggaccgcaa
     4261 ccagttagtc gtgactagca agggatgagt ccataagaat aataatcttt agaaaaatgg
     4321 tttactggta tggattattc tttataagtg agacttaccg atttagtcat caaaaaaagc
     4381 aatcaagaag aaggttttga ggtcttcaag cgttcatgat tgcttcttgt gcgttgatga
     4441 tttaggctga actccctatt taaaatcaag tttagtgatc taaaatggaa gggcctgatg
     4501 tgacatcgga ccagccaaca atggcttgtc cagtttcttc taaatactgg tttaataacg
     4561 gtttaatgtc ttgaacggta tccaaaactc ccaccagctg accttgagga tttttaagag
     4621 cttgataatg ctgcatgtaa atatgatcca atgaacccga aggaagtagg aaggttttac
     4681 aagggctatc tgataaacgc aattcagcaa gaatccaatc ttctatttcg ttatcagtag
     4741 ttggcaaaaa acttccatta aaagcatgat tatcgactag aatgttcccc tttgcatcag
     4801 ataaccttgc ttgcaaagga aggtactgta aaaaatatcc atcaaatttt tggtaaaatc
     4861 tagtgaggta ttggcttaac aataagactg ttgttgcttg atcaaagaca ctttctgtca
     4921 ctaaaaagcc ctgaccatgt tgagaggttt tgaaatagag tgtaggcaag tcaatgtcat
     4981 gctcatggaa actgaaaatt ccttggaaag tcgacaattg atttgactga atgttggctt
     5041 tgctagtgag aatagtcacc tcaaaaaggt gtgagaagtg gtgggaaagc tctctaataa
     5101 acgtatcttc aagttggttt tcaggtgtta agagcgctaa aatggaaatg gcatttttca
     5161 taattgtatg atagctgtat tttctagaaa agacaattaa tctgcttatc tatcatcata
     5221 ggcaagtcta ggatttttga taaggtaatt ttggagagat aggctgacaa agtgacctgt
     5281 ttttctataa ggggtaagga ggaatagtct cattatttga cttatagtgg gaaacttttt
     5341 ataatggtca acaacaaacg acaagaaaat aatgagagaa aatgtttttg agaattggta
     5401 gtttttacct tactgaggat acctaagagt ccctttaatt agtagatgcg tttgtctaaa
     5461 aatggaagtg cctgcagtaa ttccttgtag atacttagaa cgttttgaga aaaaatgaca
     5521 gagatacagg ttgaatgaac tcatcagctt ttatgctgtt gctttaacca atgaattgga
     5581 gagataatga tacgttttaa taatgtttct aaaacttttg gtcagacaaa ggttttgcaa
     5641 gagcagacct ttcaaattaa tgaccgtgaa ttttttgtgc tggttggccc cagcggatcg
     5701 ggcaagacca cacttttaaa aatgattaat tgccttattg aaccaagctc tggagatatt
     5761 ttactaaata acgtcccaca gaaagagtta gatttacgtg agatgaggct atctattgga
     5821 tacgtattgc agcaaattgc tctgttcccc aatctcacag tagctgaaaa tattgcgatt
     5881 atccctgaga tgaagcaatg gtcagctgaa gaaattagac aaaaaactga agaattattg
     5941 gataaggtgg gcctacctgc taaagattat cttgatcgtt acccaagtga tttatcggga
     6001 ggtgaacagc aacgtattgg tattgtacgt gctatcattt ctcatcctaa aattctattg
     6061 atggatgaac cattttcagc cttagatcct atttcacgta aacaattgca agagttaatg
     6121 ttgagtcttc acaaggaatt tgacatgacg attgtatttg tgacacatga tattgatgag
     6181 gccattaaat taggagaccg tgtagctatt ttaaatgagg gagaaattgt gcaactggat
     6241 cgcccagaaa tgattaagac ccaccctgct aatgcttttg tggtcaattt atttggaggt
     6301 gatgagcatg cctagtttgt ttgtaacttt ccaaaaccgt tttaatgaat ggctggcggc
     6361 tctgggggaa cacctgcaaa tttccctttt atctcttatg atcgccttgc taataggtgt
     6421 gcctttagca gcccttctca gtcgcagcaa acgttggtca gacattatgt tacaggtaac
     6481 aggtgttttt caaaccattc cctcactggc tttgcttggt cttttcatcc ctttaatggg
     6541 aattggaacg ttgcctgcag tgacagcttt agttatctat gcgatttttc cgattttaca
     6601 aaacaccatc acaggattaa atggtattga cccaagtctc gtggaagcag gaatagcttt
     6661 tgggatgacc aaatgggagc ggttaaaaac gtttgagatt ccaattgcaa tgcctgttat
     6721 tatgtcaggt gtgcggacgt cagcagtcat gattatcggc acagctactt tagcttcctt
     6781 gataggagcc ggtggacttg gctctttcat cttattgggg attgatcgta ataatgctaa
     6841 cctgattctg ataggggcta tttcttcagc tctgctagct attattttca atagtttgtt
     6901 acagtacctt gagaaagctt ccttgcgacg gattatgatt agttttggaa tcaccttact
     6961 tgcattgcta gcatcgtata ctcctatggc gcttagtcag ttttcaaaag gaaaagatac
     7021 agtggttatt gccggtaaat tgggagcaga gcccgatatt ctgattaatc tctataagga
     7081 attaattgaa gaccaatcag atataagtgt tgagttgaaa tctaattttg ggaaaactag
     7141 cttcttatat gaagccctta aatctggaga tattgatatg tatcctgaat ttacaggaac
     7201 cataacatca agtcttttac gcgacaaacc acctttgtct aatgacccta agcaggtcta
     7261 tgaggatgct aaaaaaggca ttgctaagca agataaactg acccttctca agccatttgc
     7321 ttaccaaaat acgtatgctg ttgctatgcc agaaaaattg gctaaggaat atcagattga
     7381 aaccatttct gatttaaaag cgcatgctga tactttaaag gctggtttta ctttggaatt
     7441 taaggacaga gcagatggct ataagggaat gcaatctcaa tatggattac agctatctgt
     7501 ggcgacgatg gagccagctc ttcgttatca agcaattcaa tcaggagata tccaagtaac
     7561 agatgcttac tctacggatg ctgaaattac gaaataccat ttgaaagttt taaaggatga
     7621 taaacagttg tttccacctt atcagggagc tcctttgatg aaaacttcct tattaacaaa
     7681 acatccagaa ttaaaaggca ttcttaatca attagcggga aaaattactg aaaaagagat
     7741 gcaggacatg aactacgagg tatctgttaa aggagcagat gctaataagg tcgctcgaga
     7801 ttatctgttg aaaacaggct taatccagaa ataaacagaa atgttggtcc gtaggactgg
     7861 cattttttcg tctcttaagt tcaagaagta acaattcata aacacaaaaa ccgaacttta
     7921 aaattaaaaa tagcatataa atttgacaaa acataaacaa ataagtaatc tataaaatga
     7981 aaggtaagtt tttacctaaa gatacctcta atcatcagag gtcataggaa gattattttg
     8041 tatagaggac ttagagaaga aggtgagaac taacttgttt ttcttaatct atgttaaaag
     8101 aggagaaact tatgtttaat gacatccctg tatttgatta tgaagatatt caactgattc
     8161 ctaacaagtg cattattact agtcgttcac aagcagatac aagtgtgaca ctcggaaaat
     8221 accagttcaa gctaccagtt atcccagcaa atatgcaaac cattattgat gaaacaattg
     8281 ctgagcagtt ggcaaaagag ggttattttt atattatgca tcgttttgat gaagatagcc
     8341 gcaaaccttt tatcaaacgc atgcatgaac aaggcttgat tgcatccatc tctgttgggg
     8401 ttaaagctta cgaatatgaa tttgtgactt ctttgaaaga ggatactcct gagtttatca
     8461 ccattgatat tgctcatggt catgctaaca gtgttattga tatgattaag catatcaaaa
     8521 ctgaattacc agaaacattt gtcattgctg gaaatgttgg tacgccagaa gctgtacgag
     8581 aactcgaaaa tgcaggtgct gatgctacta aagtaggcat cggtccaggc aaagtatgta
     8641 tcactaaagt taaaacaggt ttcggtacag gtggttggca gttagcagca ctgcgctggt
     8701 gcgcaaaggc agctcgtaag cctatcattg ctgatggtgg gattcgcaca catggtgata
     8761 tcgctaaatc catccgcttt ggagctagta tggtcatgat tggttcgctt tttgcagggc
     8821 atattgaaag tcctggaaaa acagtagaag tgaatggtga aacgtttaaa gaatattatg
     8881 gctcggcgtc agcatatcaa aaaggcgagc ataaaaatgt agaaggcaaa aaaatcttat
     8941 tgcctacaaa aggtcactta tcagataccc tgaccgaaat gcaacaagat ttgcaatcgt
     9001 ccatttctta cgcgggtggt aaagatttag acagtttgcg tcatgttgat tatgttatcg
     9061 tcaaaaattc tatttggaat ggtgattcca tttaaatgat atggagttag ctaggaccag
     9121 taaagcattt tttagcttaa tgctggagat gcttttatca tatctctgta ggtattgaaa
     9181 aaaataagaa acatggtaaa atagtaaatt atcataatga attcaataat gacatactta
     9241 tttatgctgt gaattggcgc agcgtctcta caagacacct taatgtctaa caataagtaa
     9301 gcttttaggc ttgcttggtt tttaaccttg caaagtgaga aactcttata gtttctggct
     9361 ttgcggcagg ctttttctat ttgaatcaaa aggagattta tgcagttact tgaagaacgt
     9421 atcttaacag atggcaatat tctaggagag aatattctaa aggtagataa ttttttaact
     9481 catcaagttg attaccggtt gatgaaagca attggtaaag tgtttgctca aaaatatgct
     9541 gaggctggca ttacaaaagt ggttacaatc gaagcttcag gtattgcacc agccgtatac
     9601 gctgcagaag caatggatgt tcctatgatt tttgcgaaaa aacataaaaa cattaccatg
     9661 acagaaggca ttttgacagc agaagtttat tctttcacta aacaagtgac gagcacggtg
     9721 tctatcgctg gtaaattcct atctaaagaa gacaaggttt tgattattga tgacttttta
     9781 gctaatggtc aggcagccaa aggcttgatt gagattattg gtcaagcagg ggcacaagtc
     9841 gtcggcgttg gtattgtgat tgagaaatct ttccaagatg gtcgtcgatt gattgaagat
     9901 atgggcattg aagtgacctc attagcccgt attaaaaact ttgaaaatgg taatctaaac
     9961 ttcttggagg ctgacgcata atgacacatt caacaaagca ggaacattct cactcgcaat
    10021 cagctgtcct tggcttacag catgtgcttt cgatgtatgc cggatctatt ttagtaccaa
    10081 ttatgatcgc aggtgcttta ggttattccg ctagagagtt gacttacctg atttcgacag
    10141 atattttcat gtgtggtgtg gctacttttt tacagttaaa gttaaccaag catacaggag
    10201 tagggttacc agttgtttta ggctgtgctt ttcaatcagt ggcacctcta tccattattg
    10261 gagcgcaaca aggttcaagt gccatgtttg gagccctcat tgcgtcagga atttatgtca
    10321 gtttagtggc aggtattttt tctaagattg ctcgtttctt cccaccaatt gttacaggat
    10381 cggtcattac cgttattggc ttaagtctcg taggtgttgc tatggggaat atgggagata
    10441 acgtcaaaga gcctacggca caaagcatga tgttatcatt gttgacgatt gtcattattc
    10501 ttttagtcca aaaatttacc aaaggttttg tgaaatccat ttccattttg attgggcttg
    10561 tagcgggaac tttggtttca gccatgatgg gattagtaga tacaactcct gttgtagagg
    10621 cttcttggat tcatgtgcca accccatttt attttggcat gccaactttt gaaatcactt
    10681 ctattgtgat gatgtgtatc atcgcaacgg tttctatggt tgaatcaaca ggtgtctact
    10741 tagcgctttc agatttgact aatgatcaat tagatgaaaa acgtttgcgc aatggttatc
    10801 gctcagaagg gatcgcagtc tttctcggtg ggttatttaa taccttccct tacactggtt
    10861 tttctcaaaa cgttggactt gtccaaatct caggtattaa aacccgtcgt ccgatttact
    10921 acgcagcagg tatccttgtg atcattggcc tactccctaa atttggagca atggcacaaa
    10981 tgattccaag cccagttctt ggtggagcta tgcttgttct ctttggtatg gttgctcttc
    11041 aaggaatgca aatgcttaat cgtgttgatt tccaaaagaa cgagtataat tttattattg
    11101 ctgccgtttc catctcagca ggtttaggct ttaatggcac taatcttttt gccagcttac
    11161 cagagacagc tcaaatgttc ttaacgaatg gtattgtgat cgcaaccttg acatctgttg
    11221 tcttaaattt agttttaaat ggcaaagata aacaagatga ataattaaaa aacggctaat
    11281 tgagccgttt tttctgtgat cagttttgag caaaccgtta acccctttta atgatagtat
    11341 ggttgaaaat agtgtctcaa accatttgac ataaggacat ggtgctttct tgttactaga
    11401 agaccttcaa tattatcaaa ggtgtttaaa agtgtgataa tcattgagct atcaagacca
    11461 aataaacggg tagcccatat gtcacaataa aaagaagaag gagccatgat cgttaagcta
    11521 agcatatctg tttctatagg gcagcctgtc tgacgatcta agatatgatg atactgtttc
    11581 cctttggatg taaactgcct ttcataaatt ccagaagtca caacagagtg attgttaacc
    11641 ttgatgacac caagatgttg cccacgttta gcatctggtt tttgaatacc aattcgaaaa
    11701 gtcttgtcag ggcttttcgg attaggtcca tgaacgcgta catttccacc taaattaata
    11761 agggcagagt caataccatc ttcaatcagg taggccataa tcttatcagt aatataacct
    11821 tttgctaagg ctcctaagtc taaagccatt cctacttgtt gtaaaaagac agtctgtttt
    11881 ttgtcatcta taagaacctg tctaggatct gttagagcta actgttgtga gataaggtta
    11941 tgactaggca ctctagcatc ttcaaatcca attcgccagg cttgaacaag tggcccaatg
    12001 gcgatattaa gattacttgg tgttgacaag ctatgagctt taccaatttg gattaggtta
    12061 aataaatcag agtgaacgct gacgggttta acaccagcca cctgattaat agccatcaat
    12121 tcagagtttg aatcattagc tgaaaaacga tttttgtaag tatataataa gtcaatgact
    12181 ctacttagtt gttggcaagc tttgtcagat tcgatctgaa tatcaataac tgttcccatt
    12241 agctttaatt gttgcgtaac aaacataatt agtcctcttt ttagcagaat acccccacta
    12301 aggagtgggg gaaggtcatt atgatttttg tttcatttca ccttgaggat agtaagttcc
    12361 ttcaggcata tcgttgataa aaacatggat attttcttta ggtgcttttg caatgcgtga
    12421 gactacttcg gtaacctcgc gagctaattg atttttttgt tcttgtgagc gtccctcaaa
    12481 caagtcaatt gttacaaatg gcataatgtt actcctttat gttattgggc atcattgtaa
    12541 cataatgctg tgaaaaagta tacactgttt tcaatggctg ggaaaacttc agcctttgtg
    12601 gtaaaatagg tagaatacta tagaaagtag gattttccgc tttggctcaa ctctattata
    12661 aatatgggac aatgaattct ggtaaaacca ttgaaatttt aaaagttgct cataactatg
    12721 aagaacaagg caaacctgtt gtcattatga caagtgctct tgatacacgt gatggttttg
    12781 gcatcgtctc aagccgtatt ggtatgcgac gagaagctat ccccatttca aatgacatgg
    12841 atattttcac atttattgcc caattggagg aaaaacctta ttgtgtcctg attgacgaaa
    12901 gccaattttt aagcaagcaa aatgtttatg atttggcgcg tgtggtggat gagctaaatg
    12961 ttcctgtcat ggcttttggt ttgaaaaatg atttccaaaa caatcttttt gaaggttcaa
    13021 aacatttgct attattggcc gataagattg atgaaatcaa aacaatttgc caatactgta
    13081 gtaagaaagc aaccatggtt ttaaggacag aaaatggaaa acctgtctat gaaggtgatc
    13141 agatacaaat tggtggtaat gaaacatata tcccagtttg tcgaaaacat tattttaatc
    13201 cagagattta aaaggtaatc ggttttaaag gagatacaaa gaaaaatgaa tatttatgat
    13261 caattgcagg cagttgaaga tcgttatgaa gaattaggtg agttacttag tgatccagat
    13321 gttgtttcag atacaaagcg atttatggaa ttatcacgtg aagaagctaa tactcgagaa
    13381 actgtcacag cttatagaga atataaacaa gtcattcaaa ccatttcaga tgctgaagaa
    13441 atgatcaaag atgccagtgg tgatcctgaa ttagaagaaa tggctaaaga agaacttaaa
    13501 gaatcaaaag cagctaaaga agagtatgaa gaaaaattaa aaatccttct attacctaaa
    13561 gatcctaacg atgataaaaa cattattcta gaaattcgtg gtgctgctgg tggcgatgaa
    13621 gcagcgcttt ttgctggtga tcttttaaca atgtatcaaa aatatgctga aactcaagga
    13681 tggcgttttg aagttatgga atcatccgtt aatggtgttg gtggtattaa agaagtggtt
    13741 gccatggttt ctggtcagtc agtttattct aagcttaaat atgaatcagg agctcatcgt
    13801 gttcaacgtg tcccggtgac agagagtcag ggtcgtgttc atacgtcaac tgcaactgtt
    13861 ttggtaatgc cagaagttga agaagtagaa tacgacattg atcaaaaaga ccttcgcgtc
    13921 gatatttacc acgcgtctgg tgctggtggt cagaatgtca ataaagttgc tactgcagtt
    13981 cgtatggtac acattcctac aggaatcaaa gttgaaatgc aagaagaacg tactcagcaa
    14041 aaaaaccgtg ataaagcgat gaaaatcatt cgtgctcgtg ttgctgacca ttttgcgcaa
    14101 attgctcaag atgaacaaga tgctgaacgt aaatcaacag ttggtactgg tgatcgttca
    14161 gaacgtattc gtacttataa tttcccacaa aatcgtgtaa cagatcatcg tattggtttg
    14221 acacttcaaa aattagatac tattttatct ggaaaaatgg atgaagttat tgatgcactc
    14281 gttatgtatg atcagacaaa gaaattagaa tctctcaata actaatgaac tatgctacat
    14341 taatcagaac ttatgaagat aaactagaac aaatagatga ggatcgcgaa aatctcgcct
    14401 atgtttttag agaaatcaaa gaatggtcaa gtctagatat gttgattcac caaaatcaag
    14461 ctgtgactcc tgaagatgct gttcttttgg aacatatttt ctgttctcta agtcagcatt
    14521 tatctcctca atatattaca ggtaatgctt attttcgtga tttgaagctt gcagtcgaca
    14581 agcgtgtatt aattccaagg ccagaaacag aagaattagt tgacatgatt ttagctgaaa
    14641 atcttgatgc accgttgaat gttttagata ttggtacagg tagtggagcg attgctattt
    14701 ctctcaaaaa agaaaggcct aactggcaag taacggcatc tgatatttct agagctgctc
    14761 ttgacttggc aaaagccaat gctgatgctt accagcttga tataacgttt attgaatcag
    14821 atgtttttag tctcatttca ggaacatttg atattattgt gtcaaatcct ccctacatat
    14881 cctacgagga taaggaagaa gtcagtctaa atgttttgca atcagagcct catttggcac
    14941 tttttgctaa agaaaatggc tatgctattt accgtaagat tattgaacaa gctgacaatt
    15001 acttaactaa ggaggggaag ctttactttg aaattggtta taagcaagct gaaggtataa
    15061 aagatatgtt acaagcctat tttcctcaaa gacatatacg agtcgtaacg gatatatttg
    15121 gcaaagaaag aatggtggtt gttgattatg gagtatctag ctagtatcat tgaatcaggt
    15181 gatgcccttg ttttgccaac agaaacggtt tatggtcttt ttgccaaggc tttagatgaa
    15241 aaagcagtaa atgctgttta tgacttaaaa caaagacccc gagataaagc aatgaacctt
    15301 aatgtggctg attttaatag tatcttagtc ttttcaaaag aacagccgag atacctcaag
    15361 aaattatacc aagccttttt accaggtccg ttaacgatta ttttaaaagc taatgaccag
    15421 gttccttatt ggattaattc gggtctttca acggttggtt ttcgcttacc cagtcatcca
    15481 attaccacag ctttaattca aaaaacaggc cctttaattg gtccttctgc aaacttatct
    15541 ggcaaagcta gtggtcgagt ttttgatcat attatgcaag attttgactt tcaagtgttt
    15601 ggttatgcag atgatccttt tctaacaggt aaggattcaa ccatcttaga cttatcaggt
    15661 gagcgtgctg tgattttacg tcaaggtgct atcaccaaag aagaattatt agccaacgtg
    15721 cctgaacttc gcttttaaga gaggagccgc ttggaatgtt gagaccatta agtaaaacgg
    15781 actgtcctat gctacaagaa attaacgcca aagcccttgg ctatttagtt tcgcttgact
    15841 tgttagaaag gcaatatgaa cgattgattg aggattgcca tcactatttt ttagcttatg
    15901 ccgacaagga taccaatcaa ttattaggct atgttcacgc tgaacggtat gagacccttt
    15961 atgcttctga tggtcttaat ctcttgggct tggctgtttt accagcctat cagagaaggg
    16021 gaattgggag tgccttacta agggccctgg aatcccaagc taggcaggaa ggcattgcct
    16081 ttattcgcct caattctgcc agtcatcgta aagaagcgca tgctttttac cgaaacctcg
    16141 actatgcaga tgataaaaca caattacgat tcatcaaaaa cttacaagga gatgacaatg
    16201 atttttgata aaggtaatgt tgaagacttt gataaagagc tttgggatgc cattcatgct
    16261 gaagaagaaa gacaagaaca tcatatcgaa ttgattgcat ctgaaaacat ggtttcaaaa
    16321 gctgtcatgg cagctcaagg ttctgtttta accaataagt atgcagaagg ctatccaggt
    16381 aatcgctatt atggtggaac agaatgtgtg gatattgtcg aaacgcttgc tattgaacgt
    16441 gctaagaaac ttttcggtgc cgcatttgcc aatgttcaag cccattccgg aagtcaagcc
    16501 aatgcagcag cttatatggc cttgattgag gctggtgaca ccgtattagg aatggatttg
    16561 gctgcaggtg gtcatctcac gcatggctct ccagtaaatt tttctggaaa aacctaccat
    16621 tttgtgggtt actcagtaga tgctgatact gaaatgttaa attatgaggc gattttagaa
    16681 caagcaaagg cggtccagcc aaagcttatc gttgcagggg cttcggccta ctctagaagc
    16741 attgattttg aaaagtttcg tgccattgct gatcacgtag gtgcttactt aatggtggac
    16801 atggctcaca tcgctggcct tgtagcagca ggtgtgcacc cgagtcctgt ccattatgcg
    16861 catattgtga catcaacaac ccataaaacc ttacgtggtc ctcgtggagg cttgatatta
    16921 accaatgatg aagctcttgc taaaaagatt aattcggccg ttttccctgg tttacaagga
    16981 ggacctctgg aacacgtgat tgctgccaaa gcggttgcct tcaaagaagc cttggatcca
    17041 gcctttaaag actatgcaca agctattatt gataatactg cggctatggc agcagttttt
    17101 gcacaagatg atcgtttccg tctgatctca ggtggtacag ataatcatgt ctttttagta
    17161 gatgtcacta aggttattgc taatggtaaa ctagcgcaaa atcttctgga tgaggttaat
    17221 attactctaa ataaaaatgc cattcctttt gaaaccttat ctccttttaa aacatcaggc
    17281 attcgtattg gttgtgcagc tataaccagt cgtggcatgg gagttaaaga aagccaaact
    17341 atcgctcacc tcatcatcaa agccttggtt aatcatgatc aagaaactat tcttgaagag
    17401 gttcgtcagg aagtgcgtca gttaaccgat gccttcccac tctataaaaa gtaaggaatc
    17461 atgttagata gttatataaa acgtattgtc attcatcaat tttctcccaa tgacactgag
    17521 ttactgttat cagatcgttt agtgagcatt acaccacgaa ttgatgaata tttccggaaa
    17581 aaattggcta aagtgttctc ggatgaagcc aaacggggtc agtttgaggc taataatacc
    17641 ttttttacaa ccattggtga tgatttgttg gaaacgtctg ttaccattgc ccagttatgg
    17701 aaggaagcct ttgttatttc agaggatcaa aaaaccaatg atttagtctt tgtccagttt
    17761 gataaggatg gcgaaccctt ttttgctttc ttgcgaattg ctttgaaaga acagtttgct
    17821 cacctttctg acaattatga acatccattt acggtgacgc agaataatct gccgagtccc
    17881 acccagacac cagatgaagc actagtcatt aatctcaaat ctggtcagta ctacttgatt
    17941 gaaaagcgtg tgaagcataa tggcagtttt gctaattatt tttcagaaca cctcttgaaa
    18001 gttacacctg agcaatctgt taaaaagtct atcaaaatga tagaacaaac cgctcaaaaa
    18061 attgcagagc attttaatca agatgatttt acttttcagt caaagatgaa atccactctt
    18121 tttaagcagt tagaagcaga cgacgtttta tcaccagaaa aattagctga ccaactcttt
    18181 gatgataatt taacagcccg tttaaccttt gtggatcaag tcaaagatgt cattccagaa
    18241 cctataaaaa ttagcgatat tgatcattct agacagataa aaaaactaga aaatcaaaag
    18301 ctatcgttat caaacgggat agaattaaca gtaccaaatg ccatttatca ggatgctgaa
    18361 gcggttgaat ttttgttaaa tgatgatggg acttattcaa tcttgattaa aaatatagag
    18421 gatattaaaa ccaaatagat gtttagactt ttaaagcgtg cctgcagttt tcttcttctt
    18481 tttgtcattt atcaatcctt tgttattcat cacaatgttc aacgtgtttt ggcatataag
    18541 cctatggttg agaaaacatt agcagaaaat gatacaaagg caaatgtaga cttagtctta
    18601 gctatgatat acaccgagac aaaaggagga gaagcagatg tcatgcaatc cagtgaaagc
    18661 agttcaggac aaaaaaactc gattacagat agtcaggcca gtattgagca cggggtgaat
    18721 ttactgtctc ataatcttgc tctagctgag gaagcaggag tagattcttg gacggctgtt
    18781 caggcctata attttggaac agcttatatt gattatattg ctaaacatgg aggtcaaaat
    18841 accgttgatt tagcgactac ctattccaaa accgttgtgg cgccaagcct aggcaacact
    18901 agtggtcaaa cctattttta ttaccacccc ctcgccttga tttctggagg taagctttat
    18961 aagaatggcg gcaatatcta ttactcacga gaagttcatt tcaatcttta tttaattgaa
    19021 ctgatgagcc ttttttagaa agttatcatg aaacgattac gtccatattt gaaagggtac
    19081 ctaaaagaaa gtatcttagg tcctcttttt aaattattag aagctttatt tgaattatta
    19141 gtccctttgt taattgctaa catgattgat atatcgatta gtcaacacaa cagccaggga
    19201 attttgaggg ttgttttaac attatttggt ttagcaacca ttggcttatt gctttccgtt
    19261 acagcccagt atttttcttc gaaagcagct gttggtttta caagacaaat gacagatgat
    19321 ttgtttaaaa aaatcatgtt tttgagcaag gaggaccaag accatcttgg ttatgctagt
    19381 ctggtatcac gattgaccag tgatagtttt caaattcaaa ctggtatcaa tcaatttttg
    19441 cgtctttttc taagggcccc tattatcgta tgtggtgcta tggtaatggc ttattggatt
    19501 agtccaagtc taaccctatg gtttgtgatg atggtgattg tcttactaac acttgttttt
    19561 gttatgtcac atttattagg tcccctttat ctcttaatcc gccgagaaac agatcacttg
    19621 gttcgtttga ctagccaaca gttacaagga atccgagtaa ttaaggcttt taatcaaacc
    19681 caaaaagaac tccaagcatt taagcagcaa aatatgctct taagtcgcca ccaatatcaa
    19741 gctgctaccc tagccaatgt cttgaatcct atgacttttt tagtggtcaa tcttactctg
    19801 ttaatcttga tttggcaagg aagttggcaa gtagctcata gatcgcttag tcagggcatg
    19861 ttggttgctt taattaatta cctcttgcag attttggcag aacttttgaa aatgaccatg
    19921 ttgatgggaa ccatcaatca aagtgtaaca gccgcaaaac ggattaatca ggtgtttgtt
    19981 ttagctgatg aagtgccttt gcccttactt aaagatggtc ctatttcaac tcatctgttg
    20041 accatccggc acttgacctt tacttatcca ggagcagcag agccaagtct gtatgatatt
    20101 cagttaagtg ctgatcaggg agaatggatt ggcatcattg gtggtactgg agcaggcaaa
    20161 acgactttga ttgacttgat ctgccaaact tattcacaat atagtggtga gattagtctt
    20221 aattggcaag gagaagtgcc aaaaacacta accgagtggc gaaatgtgat tgctcttgtt
    20281 cctcaaaaag ctcaattatt taaaggaacg attagaagta acctcctact gggacaatca
    20341 atgcctatta atgatgagga gctgtggcgg gctcttgaat tagctcaagc aaaagagttt
    20401 gtcgctgcct taccagaaca attagaggca cctgttgaag cctttggccg tcatttttca
    20461 ggagggcaac ggcaacgtct agctattgcc agagcacttc tgaagccaaa acccattttg
    20521 attttggatg atgccagttc cgccttagac aatgagactc gaggacgact ttttaaagct
    20581 ctaaaagaag agttatcaga tgttttagtg attctagtca cgcaatccat taagaatttg
    20641 cagtttgctg ataagatttt agtgttggaa cagggccatc aattggattt tgccagtcat
    20701 gaccagttaa aagtaagcaa tgcactttat caagaaatgc tagcgcttta cgtgaagggg
    20761 gagtaaaaat gattaaaaca gaccatcacc tcctaaagcg agtgcttcag gatttgttaa
    20821 aaaaacctct acctgtttgt attctcgtga tagctagttt tgtacaagtt ggtttaagtg
    20881 tttacttgcc tgttcttatc ggtaaagccg ttgatatgag tttgtcagtc aattcttggc
    20941 aaactttaaa atggctgttg gggcaaatgt tagttgttat tgtggtcaac acattgattc
    21001 aatgggtgat gcctcttgtc tatagtcgtt tgctatatca atatagccag caattgaaag
    21061 ataagctttt ggaaaaaatt catcgtcttc cttttgcata tctagatcgt caaaccattg
    21121 gagatttggt tagtcgagtg ataaccgaca ctgagcaact gatcaatggt ctacaattgg
    21181 tttttaacca gtttatactt ggactattga cgattctgtg taccattata gctatggcac
    21241 agattgactg gctcatgtta atcttggttt tggtgttaac accaagctcc ctctttttag
    21301 ctcgttttat tgctcaaaag agttttcatt atgcacaagc acagaccaaa agcagaggaa
    21361 atttagctca gtttactgaa gaaatccttc gtcaagaagg tttggttcaa ctgtttaatg
    21421 ctcaagagca gtctatctgt gattatcatg ttttaaataa aacctattgc gaggcttcac
    21481 aaaaagccat tttttacgcc tcaacagtca atccagccac tcgctttatt aatagtgtca
    21541 tttatgcttt actggcaggt ttaggggctg ttcgtatcat ggctggactt ttttcagtag
    21601 gtcagctaac cactttttta aatgttgttg tccaatatac caaacctttc aacgatattt
    21661 catctgtctt ggcagagata cagagttctt tagcttgtgc gcaaaggctt tatgaccttt
    21721 tagatattga aataaaagag caagagcact ttctaacatt taaggcatca gcggttaagg
    21781 gacaaattga ctttgaagaa gtaagttttt cttatcaaaa agacaggccc ttactgaagg
    21841 atattaattt ttctgttcct gcgggttcca aggtagcgat tgtagggcct acaggtgcag
    21901 gcaaatcgac tttaatcaat ttactcatgc gcttttatga acttgatgct ggtagcatca
    21961 agttagataa agttcctatt aagtgttatg ctaaggaaga acttaggtcc attactggca
    22021 tggtattgca agaaacctgg ttgaaagatg cgactgttca tgagttgatt gcttacggca
    22081 gtgaagaggc tagccgtggt gaagtagtgg cagcagccaa agcagctcat gcacacttct
    22141 ttattatgca acttcctaag acttatgata cttacttaag tgcttctgat gatgctttgt
    22201 cccaagggca gctccagtta ttagctattg ccagaatgtt tttgaaaaaa ccaaaagtct
    22261 tgattctaga tgaagccacc tcctctattg atattagaac agaagctgtt attcaagagg
    22321 cactaaaaga actcatgaga ggaaggacca gctttatcat tgcccatcgt ttatcaacga
    22381 ttcaatcagc tgatttgatt cttgttatgg atcaaggtcg attggttgag tggggaacac
    22441 atgccagctt aatgtcaaaa aacggatgtt atgctagatt acaaaagata gaataaagtt
    22501 cgtcttttaa caaaggtgaa ctttttaact cttcctaaag ttagttaatt aacaaacttt
    22561 aaactgttat ataaaaaaat aacagcgaaa aagcggacga tcttttttta taagtgagaa
    22621 aaaacagttg actaaagaag aaataactca aaaaagtgag caatatattt ggaagcgttt
    22681 tttgactgtg gtataataga gccctatata atattgtgaa atgaggaaca aaatatatga
    22741 gtaaaatcgt tgttgttggt gcaaaccatg ctggtacggc ctgtatcaag actatgttaa
    22801 caaactacgg tgatgctaat gagattgttg tatttgacca aaactcaaat atttcatttt
    22861 taggctgtgg tatggcactt tggattggtg agcaaattgc tggaccagaa ggacttttct
    22921 attcagataa agaagaatta gagtctttag gggctaaagt ttacatggaa tcacctgttc
    22981 aatcaatcga ctacgatgcc aaaacagtga cagcgcttgt tgatggtaaa aaccacgtgg
    23041 aaacttacga caagttgatt tttgcaactg gctcacaacc tatcttacca ccgattaaag
    23101 gtgccgaaat caaagaagga tcacttgaat ttgaagcaac tcttgaaaat cttcaatttg
    23161 tgaagttata ccaaaactca gctgatgtga ttgcaaagct tgaaaacaaa gacattaaac
    23221 gtgtagccgt agttggtgct ggttacattg gtgttgagtt agctgaagct ttccaacgca
    23281 aaggcaaaga agtggttctg attgatgtcg tggacacttg cttggcaggt tattacgacc
    23341 gtgatttgac tgacttaatg gctaaaaaca tggaagaaca tggtattcaa ctagcctttg
    23401 gtgaaacagt taaagaagta gctggtaatg gtaaggttga aaagatcatt actgacaaaa
    23461 atgagtacga tgtggatatg gttatcctcg ccgttggttt ccgtccaaac acaactcttg
    23521 gaaatggtaa gattgatctt ttccgtaatg gtgctttcct tgtcaataaa cgccaagaaa
    23581 cttctattcc aggtgtttat gctatcggtg actgtgcaac tatctacgat aatgctactc
    23641 gcgatacaaa ctacattgct ttagcttcaa atgctgtccg tacaggaatt gtagcagcac
    23701 ataatgcttg tggtacagac cttgaaggaa tcggtgtcca aggctcaaac ggtatttcca
    23761 tttacggctt gcacatggta tcaactggtt tgactcttga aaaagctaaa cgacttggtt
    23821 ttgatgctgc agtgactgag tatactgata accaaaaacc tgaatttatc gaacacggta
    23881 acttcccagt aaccattaag attgtttacg ataaagactc acgtcgtatc ttgggtgctc
    23941 aaatggcagc ccgtgaagat gtgtcaatgg gaattcacat gttctcactt gctatccaag
    24001 aaggcgtaac cattgaaaag ttggcattaa ctgatatttt cttcttacca catttcaaca
    24061 aaccttacaa ctacatcaca atggcagcac ttggtgccaa agactagtat ctgataggaa
    24121 aaagccagag cagttcagca gcactggctt ttttgctttt taattgttgc aacctgaaaa
    24181 gttctaaact tatgattatc ctcttatagc acgttggaaa aggaatatca ggcaaaacaa
    24241 aaagagacca aagtctcttt tgtgattagt ttttagcagc agaagcaaat tcttccttag
    24301 caaaggcttc atcaatgata gctttcaatt gatttgcaga ggcttgcatt ttttgcaatt
    24361 cagcatcgtt aagtggaatg tttactggac gaacaattcc atacgctcca acgatagctg
    24421 gttgaccaat atagcaatct tcaacacctt cgtattggcc ttcttggaaa acagaaagcg
    24481 gaagaacagc attttcatca tcaagaatag cttttgtaat acgagctagg gcaactgcaa
    24541 taccgtagaa agtagcacct tttttattga tgattgagta agcagcatca cgtacagaaa
    24601 tgaaaagatc aactaggcct tgctcatcaa tgtcacgatt agcttggagc cagtcgtaaa
    24661 gacctacacc agcaacgtta gcgtgtgacc aaacagcaaa ttcagagtca ccgtgttctc
    24721 ccatgatgta agcgtgaacc gaacgcgcat caacaccgat tttagcagcc agagcttgac
    24781 ggaaacgggc tgaatctaga gatgtacctg aaccaatgac acgctcttta gggaaacctg
    24841 agaatttcca tgttgagtaa gtcaaaacgt caactgggtt agcagcaaca agaaagattc
    24901 ctttgaaacc agatgcaacg atttgtgtaa caacctcttt attgatacga aggttttttt
    24961 caacaaggtc aaggcgtgtt tcacctggtt tttgaggagc accagctgtt aagacaacaa
    25021 gatcagcatc atgacagtct gagtagtcag ctgcatagat ttttttaggt gatgtaaaag
    25081 ctagagcgtg gcttaaatct tctgcatctc cttgagtttt ttctttaaaa atatcaataa
    25141 taccaagttc ttgagcaata ttttgtgtta ccaatgcaaa agcgtatgat gaaccgacgg
    25201 caccatcacc gaccaagatt acttttttgt gttgtttagt tgcagtcatt tctaaacatc
    25261 tccttaattt tattagagca aaagctcata cggtcttatt ttaacatatt gataattgtt
    25321 tgtcattgaa ttcagaaaaa taatgaaaat agtttcctga taacgcttcc aataagaaaa
    25381 aataaggaat ttatggtata atgaaatcta gtattgacta gaaaaggagc attccttaaa
    25441 tgcaagatcg aaatttaatt gacgtcaatc tgacaagtga aatgaaaacg agtttcattg
    25501 actatgccat gagtgtcatt gtggcaagag ctttgccaga tgtgcgtgat ggtctgaaac
    25561 ctgtgcatcg tcgtattctc tatgggatga atgaactcgg tgtgacacct gataaaccgc
    25621 ataaaaaatc tgctcgtatt acgggtgatg tcatgggtaa atatcacccg catggggatt
    25681 cttctattta tgaagctatg gttcgtatgg ctcagtggtg gagttatcgc catatgcttg
    25741 ttgatgggca tggcaatttt ggttctatgg atggtgatgg tgctgccgct caacgttata
    25801 ctgaagcgcg catgagtaaa attgcgcttg aattattaag ggatatcaat aaaaataccg
    25861 ttaattttca agataactac gatggaagcg aaagagaacc agtagtatta ccagctcgct
    25921 ttcctaattt attagtcaat ggagcgacag ggattgccgt tgggatggca actaacattc
    25981 ctcctcataa tttggcagag tctattgatg ctgtcaagat ggtaatggaa catccagact
    26041 gtacaacaag agagttgatg gaagtgatcc ctggacctga ctttccgact ggtgcccttg
    26101 ttatggggcg ctcaggtatt catagggctt atgatactgg taaaggttct atcgttttac
    26161 gctctcgaac agaaattgaa acaactcaaa caggtcggga acgtattgtg gttactgaat
    26221 ttccctatgg cgtcaataaa acgaaagtgc atgaacacat tgttcgttta gcacaagaaa
    26281 aaagactaga aggtattaca gcagttcgtg acgaatctag ccgtgaagga gtgcgttttg
    26341 ttatcgagat tcgtcgagag gcatccgcga cagttatctt aaataacctc tttaaattaa
    26401 caagccttca gacgaatttc agttttaata tgttggctat tgaaaatggt gtgcctaaaa
    26461 tattgtcact cagacaaatc attgataatt acattagtca tcaaaaagaa gttattatac
    26521 gtcgcaccca atttgataaa gataaagctg aagccagagc tcatatttta gaaggcttac
    26581 tgattgccct tgatcattta gacgaagtga ttgctattat tcgtaacagc gagacagatg
    26641 tcattgctca gacagaattg atgtcacgtt tcgacttgtc tgaacgccaa agccaagcta
    26701 ttttggatat gcgtttgcgt cgtttgactg gtttggaacg tgataagatt caatcagaat
    26761 atgatgattt gttggccttg attgctgact tatctgatac tttagccaaa ccagaacgta
    26821 ttatcactat catcaaagaa gaaatggatg agatcaagcg taaatacgct aacccacgcc
    26881 gtacagaatt aatggtcggt gaagtccttt ctctagaaga tgaagaccta attgaagaag
    26941 aagatgtttt gattaccttg tcaaataaag gttatatcaa gcgtcttgct caagatgaat
    27001 ttcgtgccca gaagcgtggt ggacgtggcg tccaaggaac tggggtcaac aatgacgact
    27061 ttgtacgtga attggtatca acaagtaccc atgatacttt gctcttcttt acgaattttg
    27121 ggcgtgtcta tcggttgaaa gcttacgaaa ttccagaata tggtcgtaca gcaaaaggtc
    27181 ttccaattgt taatctattg aaattagaag atggtgaaac cattcaaact attatcaacg
    27241 cacgtaagga agaaacagca ggtaaatcct tcttctttac aacgaaacaa ggcattgtca
    27301 aacgtacaga agtttcagaa tttaataata tccgtcaaaa cggtctccgt gctcttaagt
    27361 tgaaagaggg agatcagctg attaacgtcc ttttgacaag cggtcaggat gacattatca
    27421 ttggaactca ttccggatat tctgttcgtt ttaatgaagc aagcatccgc aacatgggcc
    27481 gatcagctac gggtgttcgc ggggttaagc ttcgtgaaga tgatcgcgtt gttggcgcgt
    27541 cacgtattca agataaccaa gaagttcttg taattaccga aaatggcttt ggtaagagaa
    27601 caagtgctac agactatcct acaaaaggta gggggggaaa aggaataaaa acagctaata
    27661 ttacccctaa aaatggtcaa ttagctggat tggttaccgt agatggtaca gaagatatca
    27721 tggtgattac aaataaaggt gtcattattc gcacaaatgt tgcaaatatt tcgcaaacag
    27781 gtcgtgctac tttgggtgtt aaaattatga aactagatgc tgatgctaag attgtaacat
    27841 ttaccttggt tcagcctgaa gacagtagta tcgatgaaat caatactgat cgtgaaaata
    27901 gtatttcaaa aaataaagat aattaatcat aacaaggagt aaaaaatggt aaaaaaacaa
    27961 aagcgtcgaa aaataaaatc catgtcttgg gcaagaaaac tactaatagt tgtactttta
    28021 attctaggtt tagcattgct ttttaacaaa cctatccgaa atacattaat tgctcgaaac
    28081 tctaataaat atcaagtaac gaaagtttcg aaaaaacaaa tcaaaaagaa taaagaggct
    28141 aagtcaacat ttgatttcca agccgtagag ccagttagta cagagagtgt cttgcaagca
    28201 caaatggcgg ctcagcaact tcctgttata gggggcattg ccataccaga gcttggcatt
    28261 aatttaccaa tttttaaagg tttaggaaat actgagctta tttatggcgc aggaacgatg
    28321 aaagaagaac aagttatggg aggagaaaat aattattctc ttgccagtca tcatattttt
    28381 ggaattacag gttcatctca aatgctcttt tcgccgcttg aaaaagcaca aaatgggatg
    28441 tccatctatt taacagataa agaaaaaatt tacgaataca tcataaaaga tgttttcacg
    28501 gtagctcctg aacgcgttga tgttatcgat gatacagctg gtctcaaaga agtgacttta
    28561 gtgacttgta cagatatcga agcaacagaa cgtattattg tcaaaggaga actaaaaaca
    28621 gaatacgatt ttgataaagc gcccgccgat gtattgaaag cttttaatca ttcttataac
    28681 caagtatcta cctagatata tcttaaatat aatatttaaa aagtaggacc atagagaaaa
    28741 tttatctcat cctacttttt ttgaaaggag aaatatgatg aaactaaatg ccattcatca
    28801 cgttgctatt attgtatctg attatcatct gtcaaaagat ttttatgtga ataaactcgg
    28861 atttgaaatt atcagagaaa attatcgtcc tgacaagcat gattacaaat tagatttatc
    28921 ttgtggtcgt attgaattgg agatttttgg taaggtgaca agtgacccct actatcaagc
    28981 tcctcctaaa cgtgtaagtc aaccagaatt taaaagtgag gcctgtggtt tgagacacct
    29041 tgcttttcgt gttaccaaca ttgagagtta tgtagatgat ctaaagagct taggaattcc
    29101 agtggagcct attaggcatg atgattacac aggtgagaaa atgactttct tttttgatcc
    29161 ggatggtttg cctttagaat tacatgaata gacttattag gttaacgact ggatcgtgct
    29221 attaaactcg ttaaaaatag ttatttgttg tatcttcttt tgcttgatta atctcttttg
    29281 atcgttaaat gatttggtta actttcaaag tgaagagcaa tttttggagt aatcttgtcg
    29341 aagtttttta atagaataac tataggaaaa gcaaaata
//
LOCUS       DAUGHY010000020        28182 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.020, whole genome shotgun sequence.
ACCESSION   DAUGHY010000020 DAUGHY010000000
VERSION     DAUGHY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28182
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.020"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            16..1188
                     /gene="sda1"
                     /locus_tag="VH752_000859"
     CDS             16..1188
                     /gene="sda1"
                     /locus_tag="VH752_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase Sda1"
                     /protein_id="HEQ8289221.1"
                     /translation="MSKHWRHLIIHSALTILATLFLGILPITENTSSTIYAESGTISN
                     NWSIEQHPNYYHVEGKAQLDIKNFPELYRTTERVYKKSGQSTKPVTVSNIHYSVLDGY
                     GRSGEAYGIITKDMIDMSAGYREKWESKPEPSGWYSYFFKNTNQRATESDYKHSPKNV
                     SKISNNIKASILLSNGNVRNGYLFDRSHLIADSLGGRPFRNNLITGTRTQNVGNNDRK
                     GGMQYIENKVLDHIKRNPKVHVYYKATPVYQGSELLPRAVLVSALSSDGFIDETVRVF
                     NNVAGFNIDYQNGGLLSSTADVDINNVEENEIETTDDEIEEGIENEPDTDALKKDNKD
                     TSLQDTVYVASNGQSDVYWYNKDSMPKTVNLEKVVEMSEQVALTRGKHHSTQENIE"
     gene            complement(1304..2500)
                     /locus_tag="VH752_000860"
     CDS             complement(1304..2500)
                     /locus_tag="VH752_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEQ8289222.1"
                     /translation="MTFLDNIKQGCLDGWTKYKILPSLTAAQAILESGWGKHAPHNAL
                     FGIKADASWTGKSFNTKTQEEYQPGIVTDIVDRFRAYDSWTDSIIDHGKFLNDNPRYQ
                     SVVGETDYKKACHAIKDAGYATASGYAELLIQLIEENDLQKWDKEILTNQKEVTMTTA
                     NEIVKYCVDLANSGMGVDKDGAYGTQCCDLPCFIVKNWFGIDLWGNAIDLLNSASAQG
                     LEVIYNAPGVNPKASDLFVMEVAGSPYGHTGAVIEDSDGYTIKTVEQNIDGNWDSLQV
                     GGPARFNTRDFTGVVGWIRLPVDHAHQTVDTAPQNSDTIVETPKSGTFTLDVVEINIR
                     RWPSLASEVVGSYKQGDTVSFDSEGYANGYYWISYLGGSGMRNYMAIGITDKDGNIIS
                     LWGKLN"
     gene            complement(2611..2796)
                     /locus_tag="VH752_000861"
     CDS             complement(2611..2796)
                     /locus_tag="VH752_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289223.1"
                     /translation="MSKWFKKVGIKTIKTMAQTAVGLIGSSVLITDINWPTMLSAVLL
                     SGLTCVLMNVSQIKDEE"
     gene            complement(2793..3092)
                     /locus_tag="VH752_000862"
     CDS             complement(2793..3092)
                     /locus_tag="VH752_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289224.1"
                     /translation="MHFDLLQAGAAAAALMSVFGLWKAVVTPFKKAMEANEFAMAQLK
                     DSLKELAYELKNLDRDREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            complement(3103..3723)
                     /locus_tag="VH752_000863"
     CDS             complement(3103..3723)
                     /locus_tag="VH752_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289225.1"
                     /translation="MRNWKVTGNYPQWDGTGAVVDTQVIITDDRGAVISEKVKKDLRT
                     ANDAEIIEAALEEFKKTAYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQKMINLQTIHVLTSGGKIDPDIYKGMLELIEPAKKGEYKEHDIFTVVDADKDTD
                     GVAGEGNLVFVYVNDKFTYDAQTLKDLEELEDKVTVIKYADLVKQD"
     gene            complement(3726..3887)
                     /locus_tag="VH752_000864"
     CDS             complement(3726..3887)
                     /locus_tag="VH752_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289226.1"
                     /translation="MQEKLLGKIINDLTLKVANLTLENAQLKAQHEIELEELNAQLDE
                     ATAPKEEGK"
     gene            complement(3896..5803)
                     /locus_tag="VH752_000865"
     CDS             complement(3896..5803)
                     /locus_tag="VH752_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEQ8289227.1"
                     /translation="MSRDPTLLIDESNLTIGSDGRAYYTFKADGDTKSVKLANNKCIG
                     TTRFNQLMIERGGKPTNYVAPVVVEGSGESTGLFKNLEGALSQLKELNLELTDTVNSQ
                     LWAKIKLTTNGMLREYHRDNITTEIVESANGIATRISEDTDKKLALINETVSGIRRDY
                     QDADRQLSSTYQAGINGLKATMANDKNDLKAVIQATAQGLSQKYDDEIQQLSAKITTT
                     SSGTTEAYESKLADLRAEFTRSHQGMRTVLESRISGLQSTQQSTAYQISQEIKNREGA
                     VSRVQQDLASYQRRLQDTEKNYSSLTQTVRGLQSTVSAPNRGLESRVTQLAGLIEQKV
                     TRGEMESYIRGAGDSIMLAIKGKLPQSKMSASEIVSAINLNGYGVRISGERIALDGNT
                     TVNGAFGAKLGEFIKLKADQIIGGTIDANKINVINLNASSIVGLDANFIKARIEHTIT
                     SLLEGKVIRARNGAMMIDLNNSTIDFNSDASINFNSNNNALVRKSGTHTAFVHFGNAT
                     PKNFTRSALYASIGITSSGDGINSASSGRFCGARFFRTASGYEHTASVDQAEIYGDTI
                     IFADDFGINRGFKMTPTGVNTLVDINKMYYAIVALARCWKHLQNVGWDTTHPNFTSAI
                     MNEHSNYMTGI"
     gene            complement(5814..6449)
                     /locus_tag="VH752_000866"
     CDS             complement(5814..6449)
                     /locus_tag="VH752_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289228.1"
                     /translation="MAVQEIPDTYFYRLDSQSIDGTLLQKASLVVDQFYIKIDDYYSF
                     YTDQDVDVYKIISSTQADVYKANKYNYQSLSKPDTSVQKPSAAMGLYQSITIDGLPIA
                     RVICNNQVIWSQSAEPQEVYNITAYVDANVSKLNDYAVIDYNQDFATYGVVWHRVDKV
                     AVEVDGKIVAKLQKPVRTVAGGGAMLSIETWTYPFDGIDKYKNPTFKISQH"
     gene            complement(6449..7504)
                     /locus_tag="VH752_000867"
     CDS             complement(6449..7504)
                     /locus_tag="VH752_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
                     /protein_id="HEQ8289229.1"
                     /translation="MTLEERIPIKVLFDRKDAAEWQKLNPVVDDGELVVELDTHRLKV
                     GDGKLNYNDLPYYEGPQGESITKVQLSENGDLSVWIGDKETKLGNIKGQKGDKGTSIA
                     DITKVGETLTITLSDDTQKTFSIPNGQKGDRGKGVESARVDEEGNLFVKFEEESEKLV
                     GNIKGQKGDKGDSLKFEDLTSDQIAQIKAKDVDLSAYATKAELKEIDVSKQLADYLSK
                     AEANSTYAKASHKHSLSDITDLNLDRYATKIELQNKASTYHRHRTSDIDGIDEYLKQT
                     DLPQDIVKQSDIRDVVRNAQLSGYVRLADIQYQLNNIGKLKDVATGQYLSVRVVDKGK
                     VPYNTSGLIVFERSGGK"
     gene            complement(7501..9483)
                     /locus_tag="VH752_000868"
     CDS             complement(7501..9483)
                     /locus_tag="VH752_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ8289230.1"
                     /translation="MITFLDHRDIEYGAISVIRHTNAVNGERSVSGEIYTNSDVLNNI
                     DRGWRLRFEDEYYVVIYAKPVDVGQKTQVSFDAIHQFFWDFSKSSIYEGLGDGSHTID
                     TYLETVFKGSGYRYKLEVGVNAFRKQSFNYKSRLDLFNEIIKATGLEFSVSGKVVRLL
                     KNIGTDLSTVVRKNFNMNDLTIEKNIDSFITYQKGFGAWTDPEDHSKGRLEAEYESPL
                     AKEYGRLEGAPLTDERFTVADNLKEALKSNVENSYKISVKIDMEDLMRAGYRCERPVA
                     GDYIMAINETLGFQERIRIVSFTSYYDATGALVKHEVTCNDIGSVKKQSVGSLSINSR
                     INQIDADIASAIEVATQALVSADGKNTVYGGTEMPKDEPKGTLKKGDILFLKVGDTTR
                     MYFWNGAEWEEPEVVNDPERWREDLEKQISEAIEKAKKAQEEINRRTDKELEEFRETL
                     KNLALPEEAIKKITDAIKVDDIPSIKQSFDDLKNKVSETSETSRLNAEIIGTDGKTRY
                     NKNLLVGNPNRVKKIDEDFIEVEANDGGFKRGETYTISFSQTCEPLNKVAITLIQANN
                     KGLKLVLTPTKAKMEPQTFEVTKDKQSIEVYPLSYKGVLTGDWYKSKQIDLTASEGQI
                     LALDMAYKAVVDAKGATITAKQSSNPKIILDGRRDR"
     gene            complement(9493..10335)
                     /locus_tag="VH752_000869"
     CDS             complement(9493..10335)
                     /locus_tag="VH752_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ8289231.1"
                     /translation="MDLLIEKESQATRLSDFGIYNIAIEDSAPLLSVSHRAVKGRSGY
                     IYDGATFTTKTLKVKGRVTVSDVEGVLDKQDELNALLVVDEPFYVTKMYPENSDLFNF
                     ELPGESTGDLQLIGSPHKPWKYRFKVILDDTIAYEFIGKTSQGLKYNLSFTLRTAELP
                     FGETKPKDITLSGGSFAYGGTAKASQLEWPFIIELTPSGGQTNFYIEIDGRRFEFKQN
                     SQLQNSDKLLLTGIATTLNGNYVNAKTNYEYFIFNPNPNKRITYKTDFLGTIRILNFV
                     ELYK"
     gene            complement(10347..14729)
                     /locus_tag="VH752_000870"
     CDS             complement(10347..14729)
                     /locus_tag="VH752_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEQ8289232.1"
                     /translation="MPGTFDGSIFADIGANTKDYEQAMARIVSTTQNAFRKAQDTAVN
                     SSNKMVQIIGQIMAQLANNGESLGKRLGSAYATGLKLSIGEIQRIAASIGEKIPEPIK
                     NGFQKIIIPVASVIDKIRGNLTGFINQTKMQLQNLVDIGKIRSAFAKAATGVDILTQR
                     ASSKLNNLSAIFETVAGKLPRPFGTAFRKIASSVSTLNSSIQSVGGKISNSLGRQVLN
                     PALQSWNNFFSSVSAKASAFANKVSTSLFGRLTSSLANLSSKIGSSLSNGFSRMSSSA
                     ATSLNGISQKFANTSSAGERLKSTVMSIVQAFSLMAVVQKAMHAITGAIDGAVSRVDT
                     MNRFPKTMALFGYSAEQSKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTS
                     LALAFNNAMIGYGATTEGAEQALRQFNQSLGSGKIQAEEFNSVSEAAPGLMSKMAEAF
                     GFGKNGVQDLKSALSDGKITAQEFADKMIELNDAQGGFAEMAQSSAGGIRTAWKNVHT
                     AVVKGVAGMISAFDEAAKANGMKTIAETLLSLKPAITSVFDTINSLIPNAVAAFARLK
                     QSINIDFSPLGASVKEVFALINTVLGDFAHTGELSGQAFDNLKAKITSLAPKVIALWA
                     VMNPASAIATIMPLLSLFGKVGLALGSLGASVGVFGGIISSGIASASGVVGAFAATLS
                     GLPGVFATAAGRGLSVLGTMTSAMSSLVSLALAAIGPAAILGLVVAGLGLINSQFGAQ
                     IDQLLNTAVTKGPSIIQGLVKGITSKIPALIASGTQLIAKFANAITVLLPVIIQAGVQ
                     LITSLVQGIGQNATSLISSAIKIIGSFVSSIASALPQLISVGMELLLNVVNGIVQNIP
                     LIIQQAQQIIGSFGNSLQANLPSIISHGIAILVNLVQGITQMLPTVLQIATQVITSFV
                     SGIVQFLPQLLQGGIQIIISLVQGIIQNLPQIVQSAVQIIQSLVSGLTQALPQIIAAG
                     IQLVVQLAVALIKGLPQIISAGIQLITGLGKAMLEAIPNALSGVWEGIKSGFSSMWDQ
                     ITGKSSTSTAKVSADATAMALNVGTQTTAMANQANTDTTSMLNSISQNTELANINATS
                     NAIQMANNVNAQTGTMSLQGLNDSMSLASGISANMSVANINATTQAQEMASGVNGATS
                     SMNLDAINHTLSLASGVGANMGTASTSATSQAQAMSSGVSNSLASMQSNSTKAASGLS
                     NSMTSEMSSAATSATSSANRLSSAVESGFNKAKTSATTSMNGIANAVKTGFSSINSTA
                     KQSMANLVSSVTSGMSRATAVSNNACQKILSIFRALAGQMSSAGAYAGQGFANGLASS
                     AGTIYAIANSIAANVAATIRRALDIHSPSRVTKTLGAFTGEGFALGMAEWIGEINSLG
                     KAYATAVTDQNWGVNSTVSTSAKVNNSGINTSLDNLSEEVRQSQLSEPIFEVHNEIVG
                     DKIYTAVKEKESREQSKDSYFVFA"
     gene            complement(14744..14977)
                     /locus_tag="VH752_000871"
     CDS             complement(14744..14977)
                     /locus_tag="VH752_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289233.1"
                     /translation="MEGLEYKQVAERENLSELSLKLRYTLNAKKVDVGKLKYDKHRLT
                     IKRSYQKASRSQADSDSSIVERIQMLNNHFQNR"
     gene            complement(15052..15507)
                     /locus_tag="VH752_000872"
     CDS             complement(15052..15507)
                     /locus_tag="VH752_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289234.1"
                     /translation="MEFTAAKRNIDIKFDFKTMFKINNKLGTINPETGERNADGVGAL
                     FFNILERNESAIVDLVRLSAGSGKKALTEDEILDAIAEAVDEEGTTEGLFAEIEKEMV
                     DSGFFRAKILKYIENMEKSARYLKAKDDTDATQIQIIEDMIGRMSNAVS"
     gene            complement(15561..16160)
                     /locus_tag="VH752_000873"
     CDS             complement(15561..16160)
                     /locus_tag="VH752_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEQ8289235.1"
                     /translation="MANTKEGTTIEITTGKPIVGKKIFYFIQSVDAKKGSKALLPAYR
                     TDGTTTMGGEYIDEQTQQGRVIEKATDEHSIDLTTYFVPTDPSVAVIEEAKKTGKSIK
                     IWEVIADESVKEQIQISESAGPKKDVYPAKFGYAKIDEIERGTGISDLVEMSYTANIV
                     GALQDGKFPLTKEEIEMLENVYGYQNPGDTTGDYDNITK"
     gene            complement(16172..16531)
                     /locus_tag="VH752_000874"
     CDS             complement(16172..16531)
                     /locus_tag="VH752_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289236.1"
                     /translation="MDYSPETLYLKKVKNRLGALGIPIYFKLPKSDVLEPFIVVGTNI
                     SDLSKTAQTGAVIDDFSLNIDAFLPGDSRLDAEEIKARLLRLLGRNNQIKAQILVDNS
                     IGREVYRVAINITETLF"
     gene            complement(16535..16879)
                     /locus_tag="VH752_000875"
     CDS             complement(16535..16879)
                     /locus_tag="VH752_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEQ8289237.1"
                     /translation="MSLIYRMRGLDRFLRSVERKQKSVRIAVDKELSKSAARIERQAK
                     ILAPVDTGWLRAQIYNEQQRLLHYRVVSPALYSIYLELGTRKMEAQPFLDPALRKEWP
                     VLMANLKKMFKR"
     gene            complement(16876..17154)
                     /locus_tag="VH752_000876"
     CDS             complement(16876..17154)
                     /locus_tag="VH752_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289238.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGHYDGFSKIIYKGKPRLIKGLAHHKNSTVIYV"
     gene            complement(17165..17521)
                     /locus_tag="VH752_000877"
     CDS             complement(17165..17521)
                     /locus_tag="VH752_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEQ8289239.1"
                     /translation="MGDKQLIDDIKLFIGISKDDGAQDELITLAISESKERVLAKLNE
                     YSETEITKIPDRLRFIVRDVAIKRFNRINSEGTVEDSEEGKTFKWDSYLKEYESTLRS
                     AAIGKVYSGKGVARFI"
     gene            complement(17533..18420)
                     /locus_tag="VH752_000878"
     CDS             complement(17533..18420)
                     /locus_tag="VH752_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEQ8289240.1"
                     /translation="MAEKNLNTMADLGDIKSIDFVNKFSKNINDLLKLLGVTRRETLT
                     NDLKIQTYKWEVTLDQTDPGEGETIPLSKVTRTKDKDYTVKWFKKRRATTAESIARHG
                     AARAITEADKRIMRELQNGIKDAFFTFLKTKPTKVKGVGLQKALSASWAKLATFNEFE
                     GSPLVSFVSPLDVANYLGDTKVGADASNVFGMTLLKNFLGMQNVIVMPSVPEGKIYST
                     AVENLVFASLNVKGGDLGGLFADFTDETGLIAAARNRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEAAAVPGIGG"
     gene            complement(18433..19002)
                     /locus_tag="VH752_000879"
     CDS             complement(18433..19002)
                     /locus_tag="VH752_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEQ8289241.1"
                     /translation="MAEENVTTETTEQVDTQKEAVEHPKQEHERTFTRADISKMMAAE
                     RTKWESEQSEAIEKARTEGERLAKLSKDERAKEEEQKRLDAIAEREKAVAEREMRIET
                     HSLLVEKGLPLDFIDIVLTTTAEEVKANIDNLQTIFDKAVEKRVNDRLTQKPPRTGNG
                     SVGMTKADIMAIEDDDERMRLIAENRNLF"
     gene            complement(19158..19424)
                     /locus_tag="VH752_000880"
     CDS             complement(19158..19424)
                     /locus_tag="VH752_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289242.1"
                     /translation="MNKRIKKKRKLETVVVMLVAENAMQAEAIKNQNKEIMELKSIVQ
                     RNALATNEEFASVKAATLDNQAAITNIAIDVNYIKKNYKRKWGK"
     gene            complement(19427..19615)
                     /locus_tag="VH752_000881"
     CDS             complement(19427..19615)
                     /locus_tag="VH752_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289243.1"
                     /translation="MGVRPSSFFISIIHHPIKTIKEENMFIWQMVLVALGVLVLILIV
                     GIAAIAVKSIIAELKKVG"
     gene            complement(19646..21091)
                     /locus_tag="VH752_000882"
     CDS             complement(19646..21091)
                     /locus_tag="VH752_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEQ8289244.1"
                     /translation="MLVVKKNQQQKSLNYWQKRQEDILSYLDRADLDVFSELQKLYNE
                     QAFELQKELFDFYTKYSEENKMTYQDVVKKLRHEDLSDYVANANKYRKQAEKDPELLK
                     RLNEQYVSARATRMDALNLELVYRAGILKGVLDSAFENHLKKAASYAYKKAMGGRSGT
                     INGPVLEELVRTPFDGYNYSEQLWGNTDNLVKNLQKKLKQGFVRGEHPRAIARDLAKQ
                     FNVANHRAETLIRTDGTMVINNATARRYLDAGLKYYRDLVRRDDRTTEICRKIAKENK
                     RKLLSELKPGINAAPYHFNCRTTLIPDEDELSIEVEPIDDKSTKYFKDVTSDWIDGNG
                     HKPQLSLLNEYVKNGTPYKVDGHNVVLDHSNYEYRVANWLSKKTGLQVDMVPRVNSPE
                     HINTPDYLVDRVPFDLKEITGSGKNVIDGNLRKAKKQATNIIFDITKTPLSFEEIMGQ
                     LEQIYMIDRRGLDISIIKNKDEVLAVLEKEG"
     gene            complement(21051..22583)
                     /locus_tag="VH752_000883"
     CDS             complement(21051..22583)
                     /locus_tag="VH752_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ8289245.1"
                     /translation="MEEKQFLAGTRFNENANKQFIMLQEDFEAIDYESKLWIDQLKNY
                     ISRFKAEQLERLKELKRYYLGDNNIKYRPAKTDKYAADNRIASDFAKYITVFEQGYML
                     GVPVEYKNENKDLQVAIDLMSVRNNEDYHNVKIKTDLSIYGRAYELLTVEEIDDKKTE
                     VKLYQLPAEQTFVIYDDTYQRNSLMAVHFYNIDYGSGKRKQIIKVYTSDTIYTYEDYN
                     LETKGTCLKEHEEHFFKGVPVNEYANNEERTGAYESVLDNIDAYDLSQSELANFQQDS
                     VNALLVIAGNAYTGADENDYLDDGRLNPNGRLAISIGFKKAQVLILDDNPNPNGVKPQ
                     AYFLKKEYDTAGSEAYKNRLVADILRFTFTPDTQDMKFSGVQSGESMKYKLMASDNYR
                     EKQERLFKKGLMRRLRLAANIWAIKGNEATTYSLVNDTSIVFTPNLPQNDNEIVTAAQ
                     NLYGIVSDQTIFEILNTVTGVDAEAELERLKEEADKKQSLPEPRLVGDASGQEEPTAE
                     KP"
     gene            complement(22599..23876)
                     /locus_tag="VH752_000884"
     CDS             complement(22599..23876)
                     /locus_tag="VH752_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEQ8289246.1"
                     /translation="MTIKQRPKINIVIKYPSKVFNKHIYDKLYNYSNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGILDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQIYLMFNPVSKVNWVYKAFFVKTPKNTVVYQTTYKDNRFLDDVTRENIEELAD
                     RNEAYYKIYALGQFATLDKLIFPKYDKQILNKDKLSHLSSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYVIEEYVRKNLTNDKIANAIKDLGYAKEEIRGDSAEKKSNQELRNLGIP
                     RMIDVTKGPGTVMQGIQYLLQYDWVVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSADRSKRMKNAKYYF"
     gene            complement(23866..24318)
                     /locus_tag="VH752_000885"
     CDS             complement(23866..24318)
                     /locus_tag="VH752_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEQ8289247.1"
                     /translation="MLGGDGKLSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARS
                     IGQENLTKPDIKAYIDERLEKLESEKIATQEEVLQYLTSIMRGDQQEKTLISVGEFGQ
                     KIVDIDVGAKDRIKAAELLGKRYRLFTDKVEMDVSSDVTINVGEWDDD"
     gene            complement(24408..24824)
                     /locus_tag="VH752_000886"
     CDS             complement(24408..24824)
                     /locus_tag="VH752_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HEQ8289248.1"
                     /translation="MSRLSNAQLKAFDEWLFDYRFIDNKIALRKLELQTDMNTEVDKN
                     IGGGRANFVSKVTEDVVARWDSDRELKGLSNFKEAVIATLNLLDEELTDIFNLRWGVG
                     SSNTWEEIAYTRHLSRKSIYRKRERILEIFSKKIGM"
     gene            complement(24821..25012)
                     /locus_tag="VH752_000887"
     CDS             complement(24821..25012)
                     /locus_tag="VH752_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289249.1"
                     /translation="MKIEFFLKQGPTKSVSNYGEICIDEVIGAMKNNDVIALNGKNNA
                     VIIPKQNISHVIIHKEVTG"
     gene            complement(25002..25853)
                     /locus_tag="VH752_000888"
     CDS             complement(25002..25853)
                     /locus_tag="VH752_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEQ8289250.1"
                     /translation="MEIKCGNCLELLAELTDGLIDMVITDPPYNISVKNNFATMGRTG
                     IDFGDWDKGFDLLSWIDIASQKLTKNGGMIIFNDWKNLGDIARHCEKNGLVVKDIFRW
                     VKDNPMPRNRDRRYITDSEYGVWVVRKKSKWVFNRKSEKYDRPEYRYPVVAGAEKTQH
                     PTQKPVALMKDIITRHTTKGAVVLDPFMGSGSTGVACLLTGRDFIGYELNKDYFNIAN
                     KRIAELTGNGGVGMNIIHRMENGIDAVGDLTTYAVPCCPECKNETYSQKHCPFCGENL
                     MEVANEN"
     gene            complement(25862..26128)
                     /locus_tag="VH752_000889"
     CDS             complement(25862..26128)
                     /locus_tag="VH752_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289251.1"
                     /translation="MTEELIKPLLEEIKRKDDRLYMQSNVIAVLMTAVIMLLMISIAL
                     QNHYEPQITGLRTQLSRTQKQLKRASEDRARQTKRIAELTGNGG"
     gene            complement(26125..26292)
                     /locus_tag="VH752_000890"
     CDS             complement(26125..26292)
                     /locus_tag="VH752_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289252.1"
                     /translation="MKKEYVVRIYTGREKNFEAKPQFEEKTFTRKADMLKFWNSCEAT
                     VKEKYTREWKK"
     gene            complement(26293..27615)
                     /locus_tag="VH752_000891"
     CDS             complement(26293..27615)
                     /locus_tag="VH752_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEQ8289253.1"
                     /translation="MRLHEYQEYAKTWIVEHPYCGLLLDMGLGKTLTTLSAIDEIQNI
                     FSEDHKILIVAPKKVAEETWPTEIEKWHFDFTYSKVLGSEGKRIEALETEADIYLINR
                     ENVTWLVEYYKTKWPFTFVVIDELSSFKSSKSKRFRALRKVRPKVQRLVGLTGTPAPN
                     SLIDLWPQIYLMDRGDRLETSQTRFKDKYFVPDKRNGPIIYSWALRDGAEAEIYNKIE
                     DICVSMKAKDYLKLPPRTNNVVSVKLSNMKAYKQLEADLVLEFKDKEISAANSAVLAN
                     KLLQMANGAIYDDDKSTVAIHDDKLDALEDIVEESQGQPILVFYQYQHDFERLKKRFP
                     QAEELTSVDKWNSGKIPILLCHPQSAGHGLNLQKGGHIIVWFGLTWSLEYYQQANARL
                     DRQGQTEPVIVHHIVAENTVDEKVLRILQGKEKNQNALLEAVKAQLGV"
     gene            complement(27612..27887)
                     /locus_tag="VH752_000892"
     CDS             complement(27612..27887)
                     /locus_tag="VH752_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEQ8289254.1"
                     /translation="MRTEKDIENYLKKKTKGLCLKFTSPGTIGVPDRIVVMNTGTFFV
                     EVKAPGKKPRPSQVAMHKKIKEAGQHVWVVDSYESVDMALKEMGNWV"
BASE COUNT         7647 a         6270 c         4936 g         9329 t
ORIGIN      
        1 aaggaggaca taaatatgtc taaacattgg agacatctaa ttattcactc tgctctgacc
       61 attctagcta cgttattttt aggtatacta ccaattactg aaaatactag tagtaccatt
      121 tacgctgaat cgggcactat ttcaaataat tggagtatcg agcaacaccc caattattac
      181 catgttgaag gtaaagcgca actggatatt aaaaattttc ccgaacttta tcgtacaacc
      241 gaaagggtct ataagaaaag tgggcaaagt actaaacctg ttacagtttc caatatccat
      301 tactctgtac ttgatggcta cggccgttct ggagaagctt atggtattat cacaaaagat
      361 atgattgaca tgtctgctgg ctatcgtgaa aaatgggaaa gcaaaccaga gccaagtggg
      421 tggtattctt atttcttcaa aaatactaac cagagagcca ctgaatccga ctacaagcat
      481 agccccaaaa atgtgagtaa gatttcgaac aatatcaaag ctagtattct cttaagtaac
      541 ggaaatgttc gtaacggcta cctgtttgat agaagtcatt taatagccga tagcttagga
      601 ggaagacctt ttagaaataa tttgattacg ggtacccgca cccaaaacgt aggtaataat
      661 gatcgtaaag gtgggatgca gtatattgaa aataaagttt tagatcacat taaaagaaat
      721 cctaaagtcc atgtttacta taaagcaact cctgtatatc aaggatccga attgctacct
      781 agagcagttt tagtgtctgc tttatcatct gatggattta ttgacgagac agttcgtgtg
      841 tttaataatg tagcaggttt taatattgat taccaaaacg gtggactctt atcttctact
      901 gctgacgtag atattaataa cgttgaagaa aatgaaatcg aaactactga tgacgagatt
      961 gaagagggaa tcgaaaacga gcctgacacg gatgcactaa aaaaagataa caaagatact
     1021 tctttacaag acactgtata tgtggcaagt aatgggcaat ctgatgtata ctggtacaac
     1081 aaagacagta tgcctaaaac tgtaaactta gagaaagttg tagaaatgag tgaacaagta
     1141 gctttgacta gaggtaaaca tcattcaact caagaaaata tagaataaca aattcaacag
     1201 accaactaga acttgaaata gtttttatat tcttattagt tatgcataag catacgaaac
     1261 acacaaaaaa ccgccctcaa tcaagagagc ggttttgtct tatctaattt aatttacccc
     1321 aaaggctgat aatgttacca tctttgtcag ttatcccaat agccatgtaa tttcgcatac
     1381 ctgagccgcc aagataacta atccagtagt agccattggc ataaccctcg ctgtcaaagc
     1441 tgacagtgtc tccttgtttg tagctaccta ctacttcact ggctaggctt ggccaacgtc
     1501 taatattaat ctccacaaca tcaagcgtaa aggtaccaga ttttggtgtc tctacgattg
     1561 tgtcagagtt ttgtggtgct gtatctaccg tctgatgagc atggtcaaca ggtaacctaa
     1621 tccagccgac aacgccagta aaatcacgag tattaaaacg ggctggtcca cctacttgta
     1681 agctatccca attaccgtca atattttgct caacggtttt aatagtatag ccatcactat
     1741 cctcgatgac agctcctgta tgtccgtagg gactaccagc tacctccatg acaaaaaggt
     1801 cactagcttt gggattgact ccaggggcat tatatatgac ctctagccct tgcgcagatg
     1861 cgctatttaa caggtctatg gcattacccc ataaatcaat gccgaaccag tttttgacga
     1921 taaaacatgg taagtcacag cattgcgtcc cataagcacc gtctttgtca actcccatac
     1981 ctgaattggc aaggtcgaca cagtatttta caatctcatt tgcggttgtc atcgttacct
     2041 ccttttgatt tgttaagatt tccttatccc atttttgtag gtcattctcc tcgattagtt
     2101 ggataagcag ctccgcatat ccacttgccg tggcataacc tgcgtccttg atagcgtgac
     2161 aggctttttt atagtcagtc tcaccaacaa cagactgata gcgtggatta tcgtttaaaa
     2221 acttgccatg gtcgataatg ctatcagtcc agctatcata ggccctaaat cggtccacaa
     2281 tatccgtgac gataccaggc tggtactcct cctgcgtctt agtgttaaaa gacttacctg
     2341 tccagcttgc atcagcttta atcccaaaca gagcgttatg tggggcatgt ttaccccacc
     2401 cgctctctaa gattgcttgt gctgcggtca aggatggcaa gattttgtac ttagtccagc
     2461 catctaagca gccttgctta atgttatcta aaaaggtcat ctgtcctcct tatctacaaa
     2521 cgggtaaaag ataagagcaa ccacagataa tggcacatac agtattgcga ttgctagtat
     2581 taacgctaat cgtgtgattg ctcgcatggc ttattcctcg tctttaattt gtgacacgtt
     2641 catcaggaca catgttagtc ctgatagcag taccgctgac aacattgttg gccagttaat
     2701 atccgtaatc aacacgcttg acccgataag accaaccgca gtttgtgcca ttgttttgat
     2761 tgttttaatt cctacttttt taaaccattt gctcattttt cttccccttt tttaaatagc
     2821 gtgataatac gctctttgtt gataataact tcgtcttcga cgcgacctaa acgctcttcg
     2881 tgacgatcga taattttttt ggtaatttca cgatcgcgat caagattttt aagctcataa
     2941 gctaactctt tgagagagtc cttgagttga gccatggcaa actcattagc ttccatggct
     3001 tttttaaaag gcgttacaac tgccttccaa agaccaaaaa cagacatgag agccgctgcc
     3061 gcagctcctg cctgtagtaa atcaaagtgc atctaaccac ctctaatcct gcttaaccaa
     3121 atcagcgtac ttgataactg tgactttatc ctctaattcc tctaaatctt tgagtgtttg
     3181 tgcatcataa gtaaacttat cattgacata aacaaagact aagttcccct caccggctac
     3241 accgtccgta tctttatcag cgtctacaac tgtaaaaata tcatgctctt tgtactcacc
     3301 ttttttagca ggttcgatga gctctaacat gcctttataa atgtcaggat caatcttgcc
     3361 accgcttgtc aaaacgtgga tggtttgcaa gttaatcatc ttttgcgtgc gttcagcgga
     3421 cactttagct agtccagcgg ctgtttgagc agtcttggca gtcttagcgg tttcttgtga
     3481 gattttttca aggtcgtcta ctttttgcac ggcttcgccc attgcaattt cgacgtaagc
     3541 agtcttttta aactcctcta aagcagcctc gataatttct gcatcattag ctgttctcaa
     3601 gtcttttttg accttctctg agatgactgc acctctgtca tctgtaataa ttacctgtgt
     3661 atcaacgact gctcctgtac cgtcccactg tgggtagttt cctgttactt tccaatttct
     3721 catgtttatt taccttcttc cttcggtgct gttgcttcat ccaattgtgc gtttaattct
     3781 tccaattcga tttcatgctg tgcttttaat tgagcatttt ccagcgttaa attagcgact
     3841 ttaagtgtta aatcgttaat aatttttcct aataattttt cttgcattgt ttctcctaaa
     3901 ttccagtcat ataattgcta tgctcgttca tgatagcgct agtaaaattc gggtgtgtag
     3961 tatcccagcc aacattttgt agatgtttcc agcaccttgc caaagctaca atcgcataat
     4021 acattttatt gatatccaca agtgtgttta cacccgttgg tgtcatttta aatccacggt
     4081 ttataccaaa gtcatccgca aaaatgatgg tatctccata aatttctgcc tgatcaactg
     4141 atgctgtatg ttcatatccg ctagccgtcc gaaaaaacct tgctccacaa aaacgaccag
     4201 atgacgcgct attgatacca tcaccagatg aagtgattcc gattgacgca taaagagccg
     4261 accttgtaaa gttttttggt gtcgcattac caaaatgcac aaaagcagtg tgggtacctg
     4321 attttcgaac aagcgcatta ttgttgctgt tgaaattgat ggatgcatcg ctattaaaat
     4381 cgatggtaga gttgtttaaa tcaatcatca tagcaccatt tcttgctctg atgactttac
     4441 cctcaagcaa acttgtaatc gtatgctcaa tccttgcttt aataaagtta gcatctaaac
     4501 caacaatgct gctagcgttg agattaatca cattgatttt gtttgcatcg attgtcccac
     4561 cgataatctg gtcggctttt agcttgataa actcaccaag ttttgcgcca aacgctccat
     4621 taaccgtggt attgccgtct aaggcgatac gctcgccaga gatgcggaca ccgtagccgt
     4681 ttaaattaat ggctgagaca atctcacttg cggacatttt gctttgcggg agtttgccct
     4741 taatcgcaag cataatactg tcgcctgccc cacgaatata actctccatt tcaccacgag
     4801 tgactttttg ttcaatcaag ccagctagtt gtgtgacacg ggattctaac cctcggttag
     4861 gggcgctgac agttgattgt aaccctctaa ctgtctgggt taagctactg taattcttct
     4921 cagtatcctg caatcgccgc tgatagctag ctaggtcctg ttgcacacga ctaacagcac
     4981 cctcacgatt tttaatctct tgtgagattt ggtaggcagt tgattgttgc gttgattgca
     5041 acccactgat tcttgactct agcactgtcc gcatgccttg atggctacga gtaaactcag
     5101 ctcgtaagtc cgcaagctta ctctcgtagg cctctgtggt gccgctagat gtcgtggtta
     5161 tcttagcaga taactgctgt atctcatcat catacttttg cgacaaacct tgagcggttg
     5221 cttgtatcac agcttttagg tcgtttttat cattggccat tgtggccttt aggccgttaa
     5281 tgccagcttg ataagttgat gatagctgtc tatcagcgtc ttgatagtca cgtctaatac
     5341 ctgagactgt ctcattaata agcgcaagtt ttttatcagt atcctcacta atccgtgttg
     5401 ctataccatt tgcactttcg acaatctctg ttgtgatgtt atcacgatga tactcacgca
     5461 acatgccgtt tgttgtgagt ttgatttttg cccaaagctg agaattaacg gtatctgtca
     5521 gctctaaatt aagctctttt aactgactga gcgctccttc caagttttta aaaagtccgg
     5581 ttgactcacc gctgccctca acaaccactg gcgccacgta gttagttggc ttaccccctc
     5641 gctcaatcat gagctggtta aagcgagtcg taccgataca tttgttatta gctaatttaa
     5701 cgctttttgt gtcaccatca gctttaaatg tataataagc acgtccgtca gagccaatcg
     5761 ttaaatttga ctcgtctatt aaaagtgttg gatctctact caattgttac ctcctaatgt
     5821 tgcgaaattt taaatgttgg gtttttatac ttgtctatcc catcaaacgg ataagtccac
     5881 gtctcgatag acagcattgc gcctccgccg gcaactgtcc gaactggttt ttgtagctta
     5941 gcaacgattt tgccgtcaac ctcaacagcg actttatcaa ctcgatgcca aacaacccca
     6001 taagtcgcaa aatcctgatt gtaatcaatg acagcataat catttaattt agatacgtta
     6061 gcgtctacat acgctgtaat gttgtaaact tcctgaggct ctgctgattg agaccaaatg
     6121 acctgattat tacaaataac gcgagctatt ggcagtccat caatagtaat cgattggtac
     6181 aaacccatcg cggcagaagg cttttggacg ctagtatctg gttttgataa tgactgatag
     6241 ttatacttat tagctttata cacatcagcc tgtgtgctag atataatttt atatacatca
     6301 acgtcttgat ctgtgtaaaa gctatagtaa tcatctatct taatataaaa ttggtcgaca
     6361 accaaactgg ctttttgtaa taaagtacca tcaatgcttt gactgtctaa gcggtaaaaa
     6421 tacgtgtctg gtatctcttg cactgccatt acttacctcc agagcgttca aaaacaatta
     6481 atccgcttgt gttatatggc actttacctt tatcaaccac acggacagat aggtactgac
     6541 ctgttgccac atcttttagc tttccgatgt tgtttaactg atactggata tcagctaatc
     6601 taacatagcc gcttagctgc gcatttcgga caacgtccct aatatctgat tgcttaacaa
     6661 tgtcttgtgg cagatcagtt tgtttaaggt attcatctat accatcaata tcagatgttc
     6721 tgtgccggtg atatgtactt gctttgtttt gtagctctat tttagtcgca taccggtcta
     6781 ggtttagatc agtaatatca cttagcgagt gcttgtgact tgctttagca taagtgctgt
     6841 ttgcctctgc tttagacaga tagtctgcaa gctgcttact cacatcaatc tctttaagtt
     6901 ctgcctttgt agcgtaggca gataggtcta cgtccttagc tttaatttgg gcgatttggt
     6961 cagaagtaag gtcttcaaac tttaaggaat cacccttatc acctttttga cctttgatgt
     7021 tgcctactaa cttttcagat tcttcttcaa acttgacaaa aaggttcccc tcttcatcaa
     7081 ctcgagcact ctctactcct tttcctctat cccctttttg gccattaggg atactaaagg
     7141 ttttttgagt gtcatctgat agtgtgatag taagcgtctc acccacttta gtgatgtcgg
     7201 caatacttgt ccctttatca cctttttgac ctttgatgtt gccaagctta gtctctttgt
     7261 cgccaatcca cacagacaag tcaccatttt cggatagctg tactttagtg atagattctc
     7321 cttgaggacc ttcgtagtaa ggcaagtcgt tgtaattcaa tttgccatct ccgaccttta
     7381 gcctgtgagt gtctagctca acaactagtt caccatcatc aacaacagga tttaattttt
     7441 gccattcggc ggcatccttg cggtcaaata agactttaat tggtattctt tcttctagcg
     7501 tcatctgtcc ctccttccgt ctaaaataat ttttggattg cttgattgtt tagctgtgat
     7561 tgttgcacct ttagcatcaa ccacagcttt ataagccata tccagagcta aaatttgccc
     7621 ctctgacgcg gttaaatcta tttgcttaga tttataccaa tcacctgtta aaacaccttt
     7681 atagctcaaa ggatagacct ctatagactg tttatcctta gtgacctcaa acgtctgtgg
     7741 ctccatctta gcctttgtcg gtgtcagcac cagcttgaga cctttattat tagcctgtat
     7801 cagcgtaatt gccactttat ttagtggctc acatgtctgg ctaaagctaa tcgtataggt
     7861 ctcgccacgc ttgaaaccac catcattggc ttctacctca ataaaatcct catcaatttt
     7921 tttaacacga ttagggttgc cgaccagcaa atttttgttg tagcgggtct taccgtctgt
     7981 gcctatgatt tcggcgttta gacgggatgt ctcgctcgtt tcactgactt tgtttttgag
     8041 gtcgtcaaag ctttgtttaa tcgatgggat gtcatcaacc ttgatagcat ctgtgatttt
     8101 tttaatggct tcttctggta acgctaggtt tttgagtgtt tctctaaact cttcaagctc
     8161 tttgtcagtg cgccggttga tttcttcttg cgcttttttg gctttttcga ttgcctcaga
     8221 gatttgtttt tcgaggtctt cacgccatcg ttctgggtcg ttcacaacct cgggttcctc
     8281 ccactcagca ccattccaaa agtacattct agtagtatca ccaactttta gaaataatat
     8341 gtctcctttt tttaaggttc cttttggttc atctttgggc atttctgtgc caccgtaaac
     8401 agtatttttc ccatcagcag atactagtgc ttgtgttgct acttcaattg cagaagcaat
     8461 atctgcatct atctgattga ttctactgtt tatagacaga cttcctacgc tttgtttttt
     8521 cacagatccg atatcattac aagtgacttc atgttttact aaagctccag ttgcgtcgta
     8581 gtagcttgta aaagaaacta tccgtatacg ctcttgaaaa cctaaagtct catttatcgc
     8641 cataatgtag tcaccagcaa ccggcctctc acatctataa ccagctctca ttaaatcttc
     8701 catatcaatt tttactgata tcttataaga attttcaaca ttacttttta aagcttcttt
     8761 tagattatct gccaccgtga agcgctcatc cgtcagaggc gccccttcaa gtcgaccgta
     8821 ctccttggca agagggcttt cgtattccgc ctcaagtctc cccttagagt ggtcctctgg
     8881 gtcggtccat gcaccaaagc ctttctgata ggtaataaag ctatcaatat ttttttcaat
     8941 ggtaagatca ttcatgttga aattttttcg taccactgtt gacagatcag tacctatgtt
     9001 ttttaatagc cgaacaactt tcccgctgac tgaaaactca agtccggtcg ctttaataat
     9061 ctcattgaat aaatctaacc tagacttata attaaaagat tgttttctaa atgcgttcac
     9121 acctacttct agcttatagc ggtacccact acctttaaat actgtctcta gataggtatc
     9181 aatggtatga gacccatctc cgagaccctc atagatacta ctcttgctga agtcccaaaa
     9241 aaattggtgt atggcatcaa acgaaacttg cgttttttga ccaacatcaa caggctttgc
     9301 gtaaataaca acgtaatact catcttcaaa tctaagtcgc caccctctat cgatattatt
     9361 gaggacatca ctgtttgtat aaatttcccc gctcactgag cgctcaccat taacagcatt
     9421 agtatgccta atgacactaa tggccccata ctcaatgtca cgatgatcca aaaaagtaat
     9481 cattagaccc tcctatttat acaactctac aaaatttaaa atcctaatcg tgccaagaaa
     9541 atccgttttg taagtaattc tcttgttagg attaggattg aagataaaat actcatagtt
     9601 cgttttagca ttgacataat ttccgtttag tgtggtagct atgcctgtca aaagtaattt
     9661 atcactattt tgtagctggc tattctgctt aaactcaaag cgcctgccat caatttcaat
     9721 ataaaaatta gtctgacctc cagagggagt aagctctata ataaaaggcc actctaactg
     9781 acttgcttta gcggttcctc cataggcaaa actgccccct gatagagtta tgtcctttgg
     9841 ttttgtctca ccgaaaggta attcagcagt tcttagtgtg aaggaaagat tatattttag
     9901 tccttgactg gtttttccta taaattcata agcgatagtg tcgtctagga tgactttaaa
     9961 tctatatttc caaggtttat gaggagagcc aataagttgc aggtctcctg tgctttcccc
    10021 tggaagctca aagttaaata gatctgagtt ttcagggtac atcttagtca cgtagaaagg
    10081 ttcgtcaaca acaagcagag cgtttagttc gtcctgtttg tccaaaactc cttctacatc
    10141 gcttaccgtc actcttcctt tgaccttaag cgttttagtc gtaaaagtag ccccatcgta
    10201 gatgtaacca ctccttcctt tgactgctcg gtgtgataca gatagtagag gagcactatc
    10261 ttcaatagca atattataaa taccaaagtc agacaatcta gttgcctgac tctctttttc
    10321 tattaataaa tccaaaatac tcccttctag gcaaaaacaa aataagaatc tttagattgc
    10381 tctcttgatt ctttttcttt aacagccgtg taaattttat ctccaacaat ctcattatgc
    10441 acttcaaaga taggctctga caattgagac tgcctaacct cttcgctaag attatcaaga
    10501 gaggtattga ttccactatt attaacttta gcagatgttg aaacagtact atttaccccc
    10561 cagttttgat cagtaactgc tgttgcgtat gctttaccta gactattgat ctcacctatc
    10621 cactctgcca taccgagggc aaacccctct ccagtaaatg ctcctagtgt tttagtgaca
    10681 cgagatggcg agtgaatatc cagagcgcgt ctaattgttg ctgctacgtt agcggcaatg
    10741 ctgttagcta ttgcgtagat tgtgcctgcg ctactagcta gaccattagc aaaaccttga
    10801 cctgcataag ctcctgcaga agacatctgc cctgccaaag ccctgaatat tgagaggatc
    10861 ttctgacaag cattattaga caccgcggtt gcacgagaca tcccagaagt cactgaactt
    10921 acaaggtttg ccatagattg ctttgcagta ctgttaatgc tactaaatcc tgttttaacc
    10981 gcattggcta tcccattcat tgaggttgtt gctgaagtct tcgccttatt gaagccagac
    11041 tcaaccgccg aggacaacct attggcgctt gaggttgcag aggttgctgc agaagacatt
    11101 tcacttgtca tgctattaga cagaccagag gctgctttag ttgagtttga ctgcattgat
    11161 gccagactgt tactcactcc tgagctcata gcttgcgcct gagaagttgc acttgtgcta
    11221 gcggttccca tgtttgcacc aacaccacta gctaagctga gcgtatgatt aatggcgtct
    11281 agattcattg aagaagtcgc accattaacc cctgaagcca tttcttgagc ctgagtagta
    11341 gcgttaatat tagccacaga catattagcg ctaatgccac tcgcaagaga cattgaatca
    11401 ttcaacccct gaagactcat cgttcctgtc tgagcattaa cattattagc catctgtata
    11461 gcatttgatg tagcgtttat attagcaagc tctgtatttt gactaatgct attaagcata
    11521 gatgtcgtat ctgtattggc ttgattagcc atagctgttg tttgagtacc aacattcaag
    11581 gccatggccg tggcgtccgc agagacttta gctgtacttg tggaactctt acctgtgatt
    11641 tgatcccaca tcgaactgaa tccgctctta ataccttccc aaactcctga aagagcattt
    11701 gggatagcct ctaacatggc tttaccgagt cccgtaatta attgaatacc cgcagaaata
    11761 atctgtggaa gtccctttat caaggctacg gctagttgta caacaagctg aatgcccgct
    11821 gcgataatct gaggcagagc ttgcgtcagt ccagaaacta aagactgtat aatctgtaca
    11881 gcagattgta caatctgggg tagattttga ataatcccct gcacaaggct aataatgatt
    11941 tgaatgccac cttgaagtaa ctgcggtaaa aactgtacaa ttccagatac aaaactcgta
    12001 ataacttgag tagctatctg cagaacagtt ggcaacattt gagtaattcc ctgtacaaga
    12061 ttcaccaaaa tagctatacc gtggctgata atactaggaa ggttagcctg taaactgttc
    12121 ccaaaactac caatgatttg ctgtgcttgc tggataataa gaggtatatt ttgaactatg
    12181 ccgttgacaa catttaataa tagctccatt ccaacagaaa taagctgcgg caaggcactc
    12241 gcaattgagc taacaaaact accaatgatt tttattgctg aactaattaa actcgttgca
    12301 ttttgtccaa taccttggac gagactggta attagctgaa cgccagcttg aattataacc
    12361 ggtaataaca ctgtgatagc atttgcaaat ttagcaatca attgagtacc actagctatc
    12421 aaagctggta ttttagaagt aatccctttt acaaggcctt gaataatgct agggcctttt
    12481 gttacagcag tgttaagtag ctggtcgatt tgtgcgccaa actggctatt aatcaaacca
    12541 agtcctgcca ccacaagtcc taggatagcg gccggtccta tggccgccag tgctaaactc
    12601 acaagactgg acattgcgct tgtcatagtt ccaagcacag atagcccacg ccctgctgcg
    12661 gtagcaaaaa cgccaggtaa tccgcttaga gttgccgcaa aggcaccaac aacgccgctg
    12721 gcactagcta tcccactaga aattatgccg ccaaacaccc cgacagaagc ccctaagctt
    12781 cctaaagcta gaccaacttt tccaaataga gaaagcaaag gcattatcgt tgctatagcg
    12841 ctagcggggt tcatcactgc ccaaagggca atgaccttag gggctaaaga agtgattttt
    12901 gcttttagat tatcaaaagc ctgccctgat aattcaccag tatgtgcaaa atctccaaga
    12961 acagtattaa taagagcaaa cacctcttta acgctggcgc ctaatgggct aaagtcaata
    13021 ttgatagact gttttagcct agcgaatgct gcaactgcat ttggaataag agaattaatc
    13081 gtatcaaaaa cgctggttat tgcgggcttc aggctaagaa gtgtttcagc aatggtcttc
    13141 ataccattag ctttagccgc ttcatcaaag gctgaaatca tacctgctac gccttttaca
    13201 acggcggtat gtacgttctt ccacgcagtt cggattccac cagccgatga ttgggccatc
    13261 tctgcaaatc cgccttgggc atcgttaagc tcaatcattt tatctgcgaa ctcttgtgca
    13321 gtgattttgc catcagataa ggctgatttt aaatcttgta cgccattttt accaaaaccg
    13381 aaggcttccg ccattttaga cattaaacct ggcgcagctt ctgatacaga gttaaactct
    13441 tcagcttgaa tttttccaga ccccaacgac tggttaaact gcctgagtgc ttgctcagcc
    13501 ccttcagttg ttgccccgta accgatcatt gcgttattga aggcgagggc taaactagtc
    13561 cctttatcta aacttcctgt ggttatggcg agctgttgag cgctttttac agcactgtct
    13621 agaggagttg gcagaccttc aattcctttt gatagcttat caattgatgc cttagattgt
    13681 tcagcagaat acccaaatag cgccatcgtt ttcggaaagc ggttcatggt atcaacgcga
    13741 ctaaccgccc cgtctattgc cccagtaata gcgtgcatag ctttttgaac aacagccatt
    13801 aaactaaagg cctgcacgat gctcatcact gtacttttga gtctctctcc agcagaagag
    13861 gtgttggcga atttctggct gattccattc aaggaagtag ccgcagaact agacattctg
    13921 ctaaaaccat tgctaagact actccctatt ttacttgata aattagctaa acttgaggtc
    13981 agccgaccaa acaaactagt actcactttg tttgcgaaag cagacgcctt agctgataca
    14041 gaactaaaaa aattattcca tgactgtaag gctgggttca agacttgccg accaagtgaa
    14101 ttagaaatct tccctccaac agactgtata gagctattca gggtagaaac ggatgaggca
    14161 atttttctaa acgctgttcc gaatggtcgc ggaagcttgc ctgcgaccgt ttcgaatatt
    14221 gcagataagt tattaagctt actacttgcc ctctgagtta agatatcaac tccagtcgca
    14281 gccttggcaa atgcagatct tatcttacca atatccacaa gattctgaag ttgcattttt
    14341 gtttgattaa tgaaaccggt taagttaccc cgtattttgt caataacact tgcaactgga
    14401 ataatgattt tttgaaaacc attctttatg ggttcgggaa ttttttcgcc aatggaagct
    14461 gctatgcgtt gaatttcacc aatactcagt tttaaaccag tagcgtaggc ggaaccaagt
    14521 ctctttccga gcgattcgcc gttgttcgct aattgggcca tgatttgtcc aatgatttga
    14581 accattttat tactactgtt aaccgctgta tcttgggctt ttctgaaagc attctgagtc
    14641 gtactaacaa tacgagccat ggcctgctca tagtcctttg tgttggcacc aatatcagcg
    14701 aaaatagaac catcaaacgt tcctggcatc tactcctcct tatctatcta ttttgaaaat
    14761 gattattaag catttgtatt ctctcaacaa tactgctatc actatcggct tggctacggc
    14821 tagctttttg atacgatctt ttgatagtta gcctatgctt atcgtatttg agcttaccta
    14881 catcaacctt tttagcattt aatgtatatc taagttttag agacaattca gagaggttct
    14941 cgcgctcagc aacctgctta tactcaagcc cctccataat agcctctagc tcccacttag
    15001 tacatttata gatagtatca atgtctgtga gccctaaccg tgcgcaattt actaagatac
    15061 tgcgttgctc attcttccaa tcatgtcttc gataatttgg atttgagttg cgtccgtgtc
    15121 gtccttcgct ttgagataac gagctgattt ctccatgttt tcgatatatt tcaaaatctt
    15181 cgctctgaaa aaaccagaat caaccatttc tttctctatt tcagcaaaca acccttctgt
    15241 tgttccttct tcatcaacag cttctgcaat tgcatctaga atttcatctt cagttagcgc
    15301 tttttttccg cttcccgcag ataaacgcac aaggtcaaca atggcgcttt cattacgctc
    15361 taagatattg aaaaacaaag caccaacacc atctgcattg cgctctcctg tttctgggtt
    15421 aattgttcct agtttattat tgatcttaaa catagtttta aaatcaaatt taatatcgat
    15481 atttcgtttc gctgctgtaa attccattat tttatctcct ttttaaatca aaatggggca
    15541 gcagttgccg cccctgtgta ctacttagtg atattatcgt agtcgccagt agtatcaccc
    15601 ggattttgat agccgtatac gttttctagc atttcaattt cttctttcgt gagagggaat
    15661 ttaccatctt gtaaggcccc tacaatgtta gcggtgtagg acatttctac taagtctgaa
    15721 ataccagtac cgcgttcgat ttcgtcaatc ttggcgtacc caaacttagc agggtaaaca
    15781 tctttcttgg gtccagccga ttcagaaatt tggatctgtt ctttgacact ttcgtcggca
    15841 atgacttccc aaattttgat agatttacct gttttcttag cttcctcgat gacagcaaca
    15901 gatggatctg tcggtacaaa gtacgttgtc aaatcaatag agtgttcatc tgtagccttc
    15961 tcaataacgc ggccttgttg ggtttgctca tcaatgtatt caccgcccat ggttgtagta
    16021 ccatcggtac gataggctgg cagcaaagcc ttgctacctt ttttagcatc aactgattgg
    16081 atgaaataaa aaatcttttt ccctacaatc ggtttacctg ttgtaatttc aatagttgtt
    16141 ccttctttag tgtttgccat ttaggtctcc tttaaaatag tgtttctgta atgttgatag
    16201 caactctata gacttctcgt cctattgaat tatctaccaa aatctgagcc tttatttggt
    16261 tattccgccc aagtagtcta agcaggcgag ctttaatctc ttctgcatcc aaacgactat
    16321 cgccaggtaa gaaagcatcg atattcaggc taaaatcgtc aataactgct ccagtttgag
    16381 ctgtttttga caagtctgat atatttgtac ctacaacaat aaaaggctct aaaacgtctg
    16441 atttaggcaa tttaaaatag attggtatgc ctaaagctcc caatctattt tttacctttt
    16501 ttaaatatag tgtttctggt gagtaatcca tgcatcacct cttaaacatt tttttgagat
    16561 tagccattag cacaggccat tcttttctca gggcagggtc taaaaacggt tgagcttcca
    16621 ttttacgagt acctaattca agataaatag aatataaagc aggagaaacc actctatagt
    16681 gtaagagtcg ttgttgctca ttgtagattt gagctctcag ccatccagta tcaaccgggg
    16741 ctagtatttt agcctgtctc tcaatcctag cagctgattt actaagctct ttatctacag
    16801 cgattcgtac tgacttctgc ttgcgctcaa cgctgcgtaa aaacctatct aggcctctca
    16861 tccgataaat aagactcata cataaataac cgtgctattt ttatggtgtg ctagtccctt
    16921 tatcaacctc ggttttccct tgtagataat cttgctaaag ccgtcgtaat ggccttgcaa
    16981 gtgcaactta aaagcattca aatcatattt accaaatagc cccatctgtt cagattttgt
    17041 gaaagtattt tccatgcagg ggattttccc gctaggtttt tcaacagttc ttttatctag
    17101 aaaatcgtcc tgctcatcga catacactaa gattactcta tctttataga tcattgtgta
    17161 tctcctaaat aaatcttgct acccctttgc ctgaatatac cttcccaata gcagcgcttc
    17221 tgagtgttga ttcatactct ttaaggtaac tgtcccactt aaaagtcttt ccttcttcgc
    17281 tatcttcaac ggttccttct gaattaatcc tattaaaccg tttaatagca acatcacgga
    17341 caataaacct caatctatca ggaattttgg tgatttcagt ttctgagtat tcattaagtt
    17401 tagctagcac acgctcttta ctttcagata tagcaagggt gatgagctca tcttgcgcac
    17461 catcatcctt ggaaatacct ataaagagtt tgatatcgtc aataagttgt ttatcgccca
    17521 tataaatagc ctttaaccac caatgccggg tacagcagca gcttcgattg ttgcctctac
    17581 aaccccttca ggaatttcag caaacagcac atttgcccca aagaaaacag attcataggt
    17641 aagattagaa agctgacgat tacgagctgc agcaattaaa cctgtctcat cagtaaaatc
    17701 agcaaacaaa ccacccaagt ctccgccttt aacatttaaa gatgcaaaaa ctagattctc
    17761 tacagccgtt gagtagattt taccctctgg cacagatggc ataacaatca cgttttgcat
    17821 acccaaaaag ttcttaagca atgtcattcc aaaaacatta gaagcatccg cacctacttt
    17881 agtatctcca agatagttgg ctacgtctaa aggagagaca aaagaaacaa gcggggaacc
    17941 ctcaaactca ttaaaagtag ctagcttagc ccatgatgca gacagcgctt tttgaaggcc
    18001 aacgccttta acttttgttg gttttgtttt gaggaatgta aagaatgcat ccttaattcc
    18061 attctgaagc tcacgcataa tgcgcttatc tgcttcagta atagcacgag ctgcgccatg
    18121 gcgagcaatt gactcagctg tagttgcacg acgtttcttg aaccacttca ctgtatagtc
    18181 tttatcttta gttcgagtaa ccttagacag agggatggtt tccccttctc caggatcagt
    18241 ttgatctaaa gtcacttccc acttataggt ctgaattttt agatcgttag ttaatgtttc
    18301 acgacgagta acccctagca attttagtaa atcattaata tttttggaaa acttgttaac
    18361 aaaatcaatt gatttaatat ctcctaagtc cgccatagtg tttaaatttt tttcagccat
    18421 aatattcctc tcttaaaata agttacgatt ctcagcaatt aaacgcatac gttcgtcatc
    18481 atcctctatt gccatgatgt cagccttagt cataccgacc gagccgtttc cagttcgtgg
    18541 tggcttctgg gttaaacgat cgttaacacg tttttcgaca gctttatcaa aaatagtttg
    18601 taaattatca atattggcct taacctcttc tgcagtagtg gttaaaacaa tatcaataaa
    18661 atccaatggc aatcctttct ccacaagcag ggaatgcgtt tcaatgcgca tctcgcgctc
    18721 tgctactgct ttttcacgct ctgcgatagc atctagacgt ttttgttcct cctctttagc
    18781 gcgctcatct tttgacaact tggccaaacg ttcaccctct gtacgagctt tttcaatagc
    18841 ttctgattgt tcagattccc atttagtgcg ttcggcagcc atcatttttg agatgtctgc
    18901 gcgagtaaaa gttcgctcat gttcctgctt aggatgttca acagcttctt tttgagtgtc
    18961 gacttgctca gttgtttctg ttgttacatt ctcttctgcc ataactttct cctagtggtt
    19021 acgccaccaa ccgatagtct agctttacgt cctgcgacga aacagtgtag cttttagtgt
    19081 catcaacaaa gtttggacac aataaaagcc gtattgctac gactttgatt tctaaagggg
    19141 tcgaattcgt ttaaaattta ttttccccac ttacgcttat aatttttctt aatgtagtta
    19201 acatcaattg caatgtttgt aatagctgct tgattatcta aagtagcagc tttaacagac
    19261 gcaaactctt cgtttgttgc cagagcgttc cgctgaacaa ttgatttcaa ctccatgatt
    19321 tctttgtttt gatttttaat tgcttctgct tgcatggcat tctctgcaac aagcatcaca
    19381 accactgttt ccaatttacg ttttttctta atgcgcttat tcatgtttat cctacctttt
    19441 tcaattctgc aataattgac ttaaccgcta ttgctgcaat gccaacaatc aatatgagca
    19501 ccaatacacc tagtgccact aataccattt gccaaataaa catattttcc tccttgatag
    19561 ttttaatagg gtgatgtata attgagataa agaaggatga tggtctaacg cccacgaagc
    19621 agtttactgt ggaggcggtg gggcatcatc cttctttttc taatacagct aatacctcat
    19681 ctttattttt gataattgat atatcaagac ccctacgatc tatcatataa atttgttcta
    19741 actgccccat tatctcttca aatgataaag gggtttttgt tatatcaaaa atgatattag
    19801 ttgcctgctt ttttgctttt cgtagattgc catcaatgac gtttttaccc gaacctgtga
    19861 tttcttttag atcaaaagga actctatcaa ctaaataatc aggagtattg atatgttcag
    19921 gggaattaac tcttggcacc atgtcaactt gcaatcctgt ctttttagat aaccaattag
    19981 caactctata ttcataattc gagtgatcaa gaacaacatt atggccatct actttgtaag
    20041 gagtgccatt ttttacatac tcatttagta gtgacagttg tggtttatgc ccatttccat
    20101 ctatccaatc tgaggtgaca tccttaaaat acttagtgct tttatcatca attggttcta
    20161 cttcaatgct tagctcatct tcatcaggaa taagggttgt tctgcagttg aaatgataag
    20221 gtgctgcatt aattccaggc tttagctcag ataatagctt tcttttgttt tctttagcga
    20281 ttttacgaca tatctcagtt gtcctgtcat cacgcctaac taaatcacga taatacttaa
    20341 gacctgcatc caagtagcgc ctagcggtag cattattaat caccatagtt ccatcagttc
    20401 tgattagtgt ttcagccctg tgattggcaa cattaaattg cttagctaaa tctctggcta
    20461 tcgccctagg atgctcgcca cgaacaaaac cttgttttaa tttcttttgg agattcttga
    20521 caagattgtc agtattgccc cacagctgtt ctgaatagtt ataaccatca aacggggttc
    20581 taaccagttc ttctaaaact ggaccattga ttgtccctga ccgtccaccc attgcttttt
    20641 tataagcata tgaggcagct ttttttaaat gattttcaaa tgctgaatca agtacacctt
    20701 ttagtatccc tgcgcgataa acaagttcaa gatttaatgc atccatcctt gtagctcttg
    20761 ctgatacata ctgctcatta agtcgtttta gcagctcagg gtctttctca gcttgtttgc
    20821 gatacttgtt agcattagct acgtaatctg ataaatcttc atgacgtagc tttttaacga
    20881 catcctgata agtcattttg ttctcttcag aatacttagt ataaaaatca aacaattcct
    20941 tttgtaactc aaaagcctgt tcattatata acttctgcag ttctgaaaag acatctaaat
    21001 cagctctgtc taaataactt agaatatctt cttgacgctt ttgccagtaa ttaaggcttt
    21061 tctgctgttg gttcttcttg accactagca tcacctacca atctaggctc cggtaaagat
    21121 tgctttttat cagcttcttc tttcaaacgt tcaagctctg cttcagcgtc tactcctgtg
    21181 acagtgttta agatttcaaa gatagtctga tcgctaacaa tgccataaag attttgtgca
    21241 gctgtaacaa tttcattatc gttttgagga agattaggtg tgaaaactat actcgtatca
    21301 tttacaaggc tatatgtagt tgcttcattc cctttgatgg cccagatatt agctgctaag
    21361 cgtaagcgcc gcattaagcc ttttttgaac aacctctctt gcttctcgcg atagttgtca
    21421 gaagccataa gcttatactt cattgattca ccagattgaa ctcctgaaaa tttcatatct
    21481 tgtgtatctg gcgtaaaagt aaacctcaaa atgtctgcaa ctagtctatt tttgtaggct
    21541 tcgctaccgg cggtatcata ctcttttttg agaaagtacg cttgtggttt aacgccattt
    21601 ggattaggat tatcgtctaa aattaacact tgggcttttt taaacccaat tgagatcgct
    21661 aaacgaccat taggatttaa tcgtccgtca tctaagtagt cattctcatc agcccctgtg
    21721 taagcattac cagctattac aagcaacgca ttgactgaat cttgttgaaa attagcaagc
    21781 tcggactgag ataaatcata ggcatcaata ttatcaagta cagactcata agcccctgta
    21841 cgctcctcat tattagcata ttcattgact ggtacacctt taaaaaaatg ttcttcatgc
    21901 tcttttaagc acgtaccttt tgtttcgaga ttatagtctt cgtaggtgta aattgtgtcg
    21961 gaagtatata ctttgataat ctgtttgcgt ttacctgagc cataatctat gttgtaaaaa
    22021 tgaacggcca tcaacgaatt tcgctggtat gtatcatcat agataacaaa agtttgctcg
    22081 gctgggagct ggtagagttt tacttcggtt tttttgtcat ctatttcctc aacagttaac
    22141 agctcgtagg ctcttccata aattgataaa tctgttttta ttttgacgtt gtggtagtcc
    22201 tcattgtttc tgaccgacat aaggtcaata gccacctgga ggtctttatt ttcatttttg
    22261 tactcaacgg gaacaccaag catataacct tgctcaaata ccgtaatata tttagcaaaa
    22321 tcacttgcaa tacgattatc cgctgcgtat ttatctgttt tagcaggtct atacttgatg
    22381 ttattatccc caagatagta tcgctttaac tctttcaaac gctctaattg ttctgcttta
    22441 aaccttgaaa tgtaattctt aagttgatct atccaaagtt ttgattcata gtcaattgct
    22501 tcaaagtctt cttgcagcat aataaattgc ttattagcat tctcattaaa acgagttcct
    22561 gctaaaaatt gtttttcttc caaagtctca cctcattcct aaaaataata tttagcattt
    22621 ttcatgcgct tacttctatc cgccgactgg tatattctgt cttgtacggc atatcttatg
    22681 gcgtcaatgc agtggttata actgtcaact ggttcgttga tatactcatt agttttcttg
    22741 tctttcttcc aagtgtaatt ttcgagttct tcaatagtct tgacacacct ttcatcaaca
    22801 acccaatcat actgaagcag gtactgaatt ccttgcataa cggttccagg cccttttgta
    22861 acatcaatca ttctaggaat acctaaattc ctcagctctt ggttagattt cttttcagcc
    22921 gaatctcctc tgatttcttc tttggcatag ccgaggtcct ttatagcatt tgctattttg
    22981 tcattggtca aattctttct gacatattcc tctatgacgt ataacttctt gtttgcgtca
    23041 tcgattttaa catgcaaaaa tgccgaaggg tcattgataa acccatagtc caaaccaaaa
    23101 aaagaagaca agtgtgataa cttgtctttg tttaatattt gcttgtcata tttgggaaaa
    23161 attagtttat caagtgtagc aaactggcca agagcatata ttttgtagta agcttcattc
    23221 ctatcggcta gttcctcaat attttctctc gtgacatcat ctaagaagcg gttgtcttta
    23281 taagttgttt gatagacaac tgtattctta ggtgtcttaa cgaagaaggc tttataaacc
    23341 cagtttactt ttgacacagg gttaaacatc aagtatattt gcttctctag atgtttctta
    23401 tctcttaaac gcaaagtcaa ctgcgtataa tcatcaagcg taaactcact agcttcttcc
    23461 atgacgacat cagatatccc cttaattgac ttaatcttct cagggttatc cataccctta
    23521 aaaatgaatt ccgagccatt cgggagcgtt attctaaaag ccgacatatt tatcttacat
    23581 ttatccaaga tgccgaaata cgacaaatta gacatgatat cagcaaatac agagtctcta
    23641 acggttgcac cgacttttct gagaactaat atctttctgg gatgtttgaa tttagggtta
    23701 agtgctttta aaattatctt ttgaaaaaca ccgtgagact ttccgctaga cgccccacca
    23761 taatgaactt ccgtgaagtt gctatagttg taaagtttgt cgtagatatg tttgttaaaa
    23821 actttgctag gatacttgat tacgatatta attttagggc gctgtttaat cgtcatccca
    23881 ctcacctacg ttaatggtta catctgagct gacatccatt tcaaccttgt cagtgaataa
    23941 tctgtaccgc ttacctagta gctcagctgc cttgattcta tcttttgcac caacatcgat
    24001 gtcaactatc ttctggccaa actctccaac gctaatgagc gtcttttctt gttggtctcc
    24061 tcgcataatt gaagtgagat attgtaggac ttcttcttgc gtagcaatct tttcagattc
    24121 aagtttttca agccgctcgt ctatataagc tttaatgtca ggtttggtca agttttcttg
    24181 acctattgac cttgctgtct ttttactata ccctgcttta atagcagctg ctgtcgcgtt
    24241 agctgagatg atgtactcat ctgcaaaacg cttctgtttt agggttaatt tactcaattt
    24301 tccatcacct ccaagcataa caaaaaggca agacactatt tgccttacct ttaaactcat
    24361 actatcaatt tatcatcaaa aagatgacaa ttccatacat ttttgtgtca cattcctatt
    24421 tttttggaga atatttctag aatccgctct cttttgcggt aaatagactt acgtgacaga
    24481 tgtcttgtat aggcaatttc ttcccaagtg ttacttgaac caacacccca cctaagatta
    24541 aaaatatcag ttagctcttc atccaaaaga tttagcgttg caataactgc ttctttgaaa
    24601 ttggatagac cctttagttc cctatctgaa tcccaccttg caaccacatc ctcagtaact
    24661 tttgaaacaa aatttgctct tcctccaccg atatttttat ctacttctgt attcatatca
    24721 gtttgtagct caagttttcg aagtgcaatc ttgttatcaa taaaacgata atcaaacaac
    24781 cattcatcaa aagcctttag ctgtgcatta gataatctac tcatcccgtt acctctttat
    24841 gaataattac gtgagaaata ttttgtttgg gtataataac tgcgttgttt tttccattta
    24901 gagcaatgac atcgttattt ttcattgcgc caattacttc atcaatacaa atttcaccat
    24961 aattagaaac gctcttagta ggaccttgct ttaaaaaaaa ttcaattttc atttgctacc
    25021 tccataagat tttcaccgca aaaaggacag tgtttctgac tatatgtttc atttttacac
    25081 tctggacaac atggtactgc ataagttgtt aaatcaccta cagcatctat cccgttttcc
    25141 attctatgga ttatattcat acctaccccc ccatttcctg tcaattctgc aatccgtttg
    25201 tttgcgatat taaagtaatc tttatttagc tcatatccaa taaagtctct ccccgtcagt
    25261 agacaagcta ctcctgttga tccgctgccc ataaaaggat ctaggacaac agcccctttt
    25321 gttgtgtgtc gcgtaatgat gtccttcatc agagctactg gcttttgtgt cggatgctgt
    25381 gttttttctg ctcctgccac aactggatat ctgtattctg gcctatcgta tttttcagat
    25441 ttacgattaa atacccactt ggattttttt cggacgaccc ataccccgta ctcgctatct
    25501 gtaatatacc ttctatctct gtttctgggc atagggttat cttttaccca cctaaaaata
    25561 tcctttacta ctaacccatt tttttcacaa tgtcttgcaa tatctcctaa atttttccaa
    25621 tcgttgaaga ttatcatgcc accgttttta gttaattttt gacttgcaat gtcaatccaa
    25681 ctcagtaaat caaaaccttt atcccaatca ccaaaatcta tacctgttcg ccccatggta
    25741 gcaaagttat tttttactga tatgttgtat ggtgggtccg ttatcaccat gtcgatcaat
    25801 ccatcagtta actccgctag taattctaag caatttccgc attttatttc catgtgtttt
    25861 cttacccccc attccctgtc aattccgcaa tccgttttgt ctgtctagct ctatcctcac
    25921 tcgcacgttt aagctgcttt tgtgtcctgc ttagctgtgt ccgtaatcct gtaatttgcg
    25981 gctcatagtg gttttgtaag gctatactta tcatcagtaa cataataact gctgtcatta
    26041 agacggcaat tacattagac tgcatataca atctatcatc tttccgcttt atctcttcta
    26101 gcagcggttt tattagttct tctgtcattt tttccactcc ctagtatatt tttctttaac
    26161 tgttgcttcg caagaattcc aaaacttcaa catgtccgct tttcgcgtaa aggttttctc
    26221 ttcaaactga ggttttgcct caaaattctt ttctctaccc gtgtagattc taacgacata
    26281 ttcttttttc atctagactc ctaactgcgc cttaactgct tcgagtaagg cgttctgatt
    26341 tttttctttg ccttgtaaaa tcctaagtac tttttcatca actgtatttt ctgcaacaat
    26401 gtggtgcaca ataacgggtt ctgtctgccc ttgtctatct aatctggcat tagcttgctg
    26461 ataatattca agactccacg ttagcccaaa ccaaacaata atatgcccgc ctttttgtag
    26521 attaagccca tgcccagctg attgagggtg acacagaaga attggtattt tcccggaatt
    26581 ccacttgtca actgacgtca actcttcagc ctgagggaat cgtttcttaa gtctttcaaa
    26641 atcatgttga tactgataaa aaactaagat tggctggcct tggctttctt ctactatgtc
    26701 ctcaagcgcg tcaagtttgt catcgtgtat agcaactgtt gatttatcat catcatagat
    26761 agcaccatta gccatttgaa gtaatttatt ggccaagacc gcagagttag ccgcagatat
    26821 ttccttatcc ttaaactcca acaccaaatc agcttcaagc tgtttgtaag ctttcatatt
    26881 agataactta actgatacaa cgttgttggt tcgcggcggt aacttgagat agtctttagc
    26941 tttcatgctg acacagatgt cctcaatctt gttatagatt tctgcttctg cgccatccct
    27001 aagcgcccaa ctgtaaatga ttggaccatt acgcttatca ggaacaaaat acttgtcttt
    27061 aaatcgagtc tggctagtct caagcctgtc gcctctgtcc atcagataaa tctgcggcca
    27121 caaatcaatc aaactgttag gcgctggggt tcctgttagt cctacaaggc gttggacttt
    27181 cggtctaact tttcgcaaag ccctaaaccg ttttgactta ctagacttaa agcttgacag
    27241 ctcatcaata accacaaagg taaacggcca tttagtcttg tagtattcaa caagccaggt
    27301 aacattctca cgattaatca aatagatatc agcttctgtt tctaacgcct caattcgttt
    27361 tccctcgctc cccaaaactt tagagtaggt gaaatcaaaa tgccatttct caatctccgt
    27421 aggccacgtt tcttccgcca cttttttagg ggctacgatt aaaatcttat gatcctcgga
    27481 aaaaatattt tgaatctcat ctatcgccga tagtgttgtc agcgttttac caaggcccat
    27541 gtcaagtaaa aggccgcaat aagggtgctc tactatccat gtcttagcgt attcctgata
    27601 ctcgtgcagt ctcacaccca gttccccatt tcttttaagg ccatgtccac tgattcgtag
    27661 gagtcaacaa cccaaacatg ctgcccagcc tcttttattt ttttgtgcat tgcaacttgg
    27721 ctgggtcttg gttttttacc aggcgctttg acctctacaa aaaaggttcc cgtattcatg
    27781 acaacaattc tgtcaggcac tcctatcgtc cctggactag taaattttaa acacagccct
    27841 tttgttttct ttttcaaata attttcaata tctttttcag tcctcatcct tcctcctttg
    27901 gtcaataaag gtcagggtta ccgttttttt caacttactt tctctttttt atatatgtgt
    27961 tttatatatg ccttatttta tatatattta ttttttatat ttatatttaa gttaatagaa
    28021 gaaaagtggt agattggtaa ccacaggggc taaactattg ctatgaaaga cctcttaggg
    28081 ctaccaaaaa gctaccgggg ttacttttcg aacccatttc taagggtcga gttactaagt
    28141 taccgatttt tccaattcgg ttttggtttt ttaaaaatta tt
//
LOCUS       DAUGHY010000021        27070 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.021, whole genome shotgun sequence.
ACCESSION   DAUGHY010000021 DAUGHY010000000
VERSION     DAUGHY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27070
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.021"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(493..1619)
                     /locus_tag="VH752_000893"
     CDS             complement(join(493..1385,1385..1619))
                     /locus_tag="VH752_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ8289255.1"
                     /translation="MSTVKRYDEEFKQSLVNLYQTGKTQSELCKDYGVSASALAKWIK
                     QYSQVKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHATTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYRLMKNMALPKMATVKPKTALKRTQKTYPQNLLNQKFNPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLELALNKRKI
                     EGTLLFHSDQGSQFKARKLRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KKYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEEN"
     gene            1923..2720
                     /locus_tag="VH752_000894"
     CDS             1923..2720
                     /locus_tag="VH752_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ8289256.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYHGEILFADMMTKEQYTEVAKKVLANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            2724..3548
                     /locus_tag="VH752_000895"
     CDS             2724..3548
                     /locus_tag="VH752_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ8289257.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAAREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDITYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKAVADAVTTLTNDESGVAEAVKTFILEEES"
     gene            3548..5098
                     /gene="ftsY"
                     /locus_tag="VH752_000896"
     CDS             3548..5098
                     /gene="ftsY"
                     /locus_tag="VH752_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEQ8289258.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQDK
                     IEVVQQEDVSSEGAGSVENGPEAASVNAQVEEETGDNSNHSSEDTNEFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATYDTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEMLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEKLVDIYEKDGRYNEAINYQDGLTVMLFVGVNGVGKTTSIGKLAY
                     RYKQEGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVA
                     KEVDILLIDTAGRLQNKENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQA
                     KEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFM
                     KGLLEGIL"
     gene            complement(5153..6520)
                     /locus_tag="VH752_000897"
     CDS             complement(5153..6520)
                     /locus_tag="VH752_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ8289259.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFNWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLGLFILSLILFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGARLSLYLG
                     NALFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            6848..7690
                     /locus_tag="VH752_000898"
     CDS             6848..7690
                     /locus_tag="VH752_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEQ8289260.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNRFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSE
                     MSYDDDDAELLIVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            7692..9554
                     /locus_tag="VH752_000899"
     CDS             7692..9554
                     /locus_tag="VH752_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ8289261.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKVKDQQ
                     VKSISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLPMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPVLAATIAGILLHPAFVAMVAEGKPLALFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFTNALIAGAATIVLAFSLTWFMGIDEE
                     SPEQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GEVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDFVK
                     RGQALLKMDIDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKIVTKEDALLVIL"
     gene            9573..10997
                     /locus_tag="VH752_000900"
     CDS             9573..10997
                     /locus_tag="VH752_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ8289262.1"
                     /translation="MGIFPKDFLWGGAVAANQVEGAFEADAKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMGFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFDELLNYGIEPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTAWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(11096..11911)
                     /locus_tag="VH752_000901"
     CDS             complement(11096..11911)
                     /locus_tag="VH752_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEQ8289263.1"
                     /translation="MIRFLILSGYFELTMYLQLSGKLDQYINVRYSYLAYISMVLSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFLDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLAEVHQTKEPNNPYVYRVF"
     gene            complement(11911..12813)
                     /locus_tag="VH752_000902"
     CDS             complement(11911..12813)
                     /locus_tag="VH752_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEQ8289264.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKAFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            complement(12961..13116)
                     /locus_tag="VH752_000903"
     CDS             complement(12961..13116)
                     /locus_tag="VH752_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289265.1"
                     /translation="MAITHKKNDELEKMLAGFASIPSFDRPLEVNTDDKLATKEKTTE
                     QTKPSKP"
     gene            13350..15482
                     /locus_tag="VH752_000904"
     CDS             13350..15482
                     /locus_tag="VH752_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEQ8289266.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYNFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKLL
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWVSKIDLDRHKVNLSLLPPRDTH"
     gene            15469..15906
                     /locus_tag="VH752_000905"
     CDS             15469..15906
                     /locus_tag="VH752_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEQ8289267.1"
                     /translation="MTLTNYVQEVSLADFGKPFHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEEHGELIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVPTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            15970..16242
                     /locus_tag="VH752_000906"
     CDS             15970..16242
                     /locus_tag="VH752_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEQ8289268.1"
                     /translation="METKFYKQRKNRLVAGVIAGLADKYGWDLALARVLAALLIYGTG
                     FGVLLYILLAIFLPYKEDLLEERYGRGPRRRKDADVLNEEEDGWFW"
     gene            16607..17539
                     /locus_tag="VH752_000907"
     CDS             16607..17539
                     /locus_tag="VH752_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEQ8289269.1"
                     /translation="MTVTVKMLVQKVKLDVVYATDNLLSKEITTSDISRPGLEMTGYF
                     DYYAPERLQLFGMKEWSYLTQMTSHNRYSVLKEMFKKDTPAVVVSRNLAIPKEMVQAA
                     KEEGISLLSSRVSTSRLAGEMSYFLDASLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAIYLENFEAGKVFDRLGNGNEEITFSGVRIPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKEMGFDATKTFEDRLTQLITKNEVSQ"
     gene            17536..18315
                     /locus_tag="VH752_000908"
     CDS             17536..18315
                     /locus_tag="VH752_000908"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEQ8289270.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESLWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            18337..18744
                     /locus_tag="VH752_000909"
     CDS             18337..18744
                     /locus_tag="VH752_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEQ8289271.1"
                     /translation="MDLVGISLMIIALGFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDVNGKVATIDPLFVAIADLSESLSDLNSQARHFGQK
                     ATNATGNVSKAGKLALVGKVASKVFGKKGEKHE"
     gene            18737..19165
                     /locus_tag="VH752_000910"
     CDS             18737..19165
                     /locus_tag="VH752_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEQ8289272.1"
                     /translation="MNKSFKNLVIGAVSGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     LAEVEAKEKQDDVIIDLNEEDIIIDYTEQDEPVSDTLDKH"
     gene            complement(19325..19609)
                     /locus_tag="VH752_000911"
     CDS             complement(19325..19609)
                     /locus_tag="VH752_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEQ8289273.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLLAKVIKSLLM"
     gene            19808..20734
                     /locus_tag="VH752_000912"
     CDS             19808..20734
                     /locus_tag="VH752_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ8289274.1"
                     /translation="MEKIIITATAESIEQVKALLAAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDQIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            20836..22122
                     /locus_tag="VH752_000913"
     CDS             20836..22122
                     /locus_tag="VH752_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ8289275.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIVICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFYAIKEELIDELWKVAQRELATGFYYGIPTENEQLFGARRKIPQYKFVGEVV
                     AFDSASMTATIRQRNVIMEGDRIECYGPGFRHFETVVKDLHDADGQKIDRAPNPMELL
                     TISLPREVKPGDMIRACKEGLVNLYQKDGTSKTVRT"
     gene            22332..22544
                     /locus_tag="VH752_000914"
     CDS             22332..22544
                     /locus_tag="VH752_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEQ8289276.1"
                     /translation="MAEFTFNIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
     gene            complement(22641..22781)
                     /locus_tag="VH752_000915"
     CDS             complement(22641..22781)
                     /locus_tag="VH752_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289277.1"
                     /translation="MTKEYISYISATIFLLYGLLAAKWIFLGLGLILVLVGLADRLNS
                     KK"
     gene            complement(22917..24410)
                     /gene="lysS"
                     /locus_tag="VH752_000916"
     CDS             complement(22917..24410)
                     /gene="lysS"
                     /locus_tag="VH752_000916"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEQ8289278.1"
                     /translation="MSNQHIEELNDQQIVRREKMMALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVD
                     FWPEMTVEEAIALAKEKQVPLEKHFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            24584..25486
                     /locus_tag="VH752_000917"
     CDS             24584..25486
                     /locus_tag="VH752_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEQ8289279.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKHVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAK"
     gene            complement(25594..26217)
                     /locus_tag="VH752_000918"
     CDS             complement(25594..26217)
                     /locus_tag="VH752_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ8289280.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVDFDAVYTSDLQRAMATAAIILDAFDQQPKLYHTDQLREWRLGKLEGAKIATMAAIY
                     PQQMLAFRENLAQFKPDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLAKGGLDNTSLTILETKDYLTYDCLIWNDKSFIEV"
     gene            complement(26558..27037)
                     /locus_tag="VH752_000919"
     CDS             complement(26558..27037)
                     /locus_tag="VH752_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEQ8289281.1"
                     /translation="MAKKTKLKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
BASE COUNT         8401 a         4799 c         5827 g         8043 t
ORIGIN      
        1 tttttaacat gaattatatt cgagacatca ttatattatt gttcgattag atggaagtat
       61 ggaatacaga ttttcatctg tagctttaaa aaataaaatt aaaagcatac ttgatagttg
      121 atgtatactg tcatttagaa taataatcac atattgttgt caacgaaacc caccatagtc
      181 tataatgaaa gcctagtagg gatgagctat ataccaacta tctatcaatc cagacgtctc
      241 tactaaaaaa tactccgtct tctatgaaac tagaaacgga gtatttctgt ttgtctatac
      301 cactaaatga acagttatat agaaaggtga ttggatgttt ggaataaaca tcatgaaaaa
      361 aatttgagac cttcttatat cctaaactag aacgaatgtc tagtgaaata aaaaaaaacg
      421 acaccattag gatgtatttt ggactaatgt caaataagta gacagaaaac cgtgttattt
      481 tattacgtta aactaatttt cttctttctg attaggggtt agtcctagat tagccgtatg
      541 tgggttgtaa ttgttataaa aattctcaat gtattcaaag cagtctaatt gaacctgttt
      601 gatattttga tacttttttc ggttgatttg tctatgcttt aaatacttga aaaatgcttc
      661 agttacggca ttatcataag gatatccagg tttagaaaaa gaatgcatga tagtgttgtc
      721 atcaattatt tttctcaatt tcctggcctt aaattgtgac ccttggtcgg aatgaaagag
      781 aagtgttcct tcaatctttc ttttattaag agctaattct agagtgtcac atgctaactt
      841 tgcatccata tgatgactca gtttccaagc aatacatttt ctagaataga gatcaagtat
      901 tgcgcagaga tagacatatt tcttatatcc aatagaaata taggtgaagt cagtagacca
      961 tacttgattt ggtttgtcag gattaaattt ctggttgagt aagttttgag gatacgtttt
     1021 ttgagtcctt ttaagagctg tttttggctt aacggtagcc attttaggga gcgccatatt
     1081 cttcataaga cggtaaattc ttccttcaga gatttttgtg tcgtagtctc tttgaagaat
     1141 gaccttgata gacttcacac caagtctttt gtttgctttg gtataaatct caagtatttg
     1201 ttttctataa atttgattat ccaattctct tttggagggc ttatgtttta gaaatttata
     1261 gtaagtggag cgattgacac gtaaaacacg acataaggtt gttgtggcgt gttcaaagcg
     1321 taaccgatag acagcgagga gtcttacttt aagttttgca tgaatatggc acttgctttt
     1381 tttaggataa ggttctcctc ttcaagctgc gcattacgtt tttgtagctc ttgtatttgc
     1441 ttggcagtaa gtacagaatt atcttcgagt ttgacttgag aatactgttt gatccatttt
     1501 gcaagcgcag aagcggatac cccatagtct ttacagagtt cagactgagt ttttccagtt
     1561 tgataaaggt tgacaagaga ttgtttgaat tcttcatcgt agcgtttaac tgttgacata
     1621 ggttgtcctt tcgtttttgt gtcttttaaa acagattgta acacacaaag ttttgtctac
     1681 ttttatagta tacatccagt ggtcatttat aaaaataaaa aagtagcagt tcacttcaaa
     1741 aacggtcagg ttatcgaaat ataatctggc tgttttttac gaatggaatg aaagctgctt
     1801 tttatgatat aataagaaaa attgaacaga atgcagaaat gatggggctt atttaaatcg
     1861 ttaaaaagtg cacaccctaa atggcgtttg caccccccta gagtaaaaaa aggagacatt
     1921 gtatgattaa actaatagca acagatatgg atggtacctt cctagctgaa gatggtactt
     1981 ataatcaaga gcaactagct gcgctcttgc caaagttagc cgaaaaaggc attttatttg
     2041 cggtttcgag tggccgctcg ctcttagcca ttgatcagct ctttgagccc tttttggatc
     2101 agattgctgt tattgcagaa aatggttccg tggttcaata ccatggtgag attctatttg
     2161 cagatatgat gaccaaagag cagtataccg aagttgcaaa aaaggtttta gccaatcctc
     2221 actatgtaga aacaggtatg gttttttctg ggcaaaaggc agcctatatt ttaaaaggcg
     2281 catcagaaga gtacattcaa aaaacaaaac attactatgc taatgttaag gttattaatg
     2341 gttttgaaga tatggagaat gacgccattt ttaaagtttc taccaacttc acaggccata
     2401 ccgtcttaga aggaagcgat tggttgaatc aagccctccc ttatgccact gcagtgacca
     2461 ccggctttga ttccattgat attatcttaa aagaggtgaa taagggcttt ggtatggagc
     2521 atctttgtca agccttgggc attaagaaag cagaaacgat tgcttttggg gataatttta
     2581 atgattacca gatgttggag tttgctggta gggctattgc tacggaaaat gcaagacctg
     2641 aaatcaaagt gatttcagac caagtcattg gccactgtaa tgacggagca gtactaacgt
     2701 atttaaaagg actagtgtaa ctcatgaata ttagaatttt ggcgcttgat ttagacggta
     2761 ccctctacaa cactgaaaaa attgtcacag atgccaataa aaaagcctta gcagcggcta
     2821 gagaaaaagg ggttaaggtg gttattacca caggtcgccc tctaaaggcc atcggtaact
     2881 tgctagaaga gttggacttg cttgaccacg atgactacag tattaccttt aatgggggtt
     2941 tggttcagcg aaatacaggg gaggttttgg ataagagctc tctcagtttt gaccaggttt
     3001 gccagattca gcaagcttta gaggcggttg gcttaccaac agatattatc agtggtgggg
     3061 atgtgtatag cattccaagt aaggacggta ggcattcgca gtaccatcta gctaacccac
     3121 tgttgacctt tattgaggtg acatctgtag cggaactacc aaaagacatt acctataaca
     3181 aaattgtgac agtcaccgat ccagactttt tggatcagca gattataaag ctatccccaa
     3241 gcttatttga ggattttgaa gcctttaaat cacgagatat tatttttgaa atcatgccaa
     3301 aaggcattga taaagctttt ggattaaact tgctttgtca gcaccttggc cttgacgcta
     3361 gacatgtcat ggctatggga gacgaagcta acgactttgc gatgttagaa tgggcaggac
     3421 ttggagtagc tatggctaat ggtgtttcag gagctaaagc cgttgccgat gccgtgacca
     3481 ccttgaccaa tgatgagtct ggggttgcag aagctgtgaa gacatttatt ttagaagagg
     3541 agagttaatg ggattatttg atcgtttatt tgggaaaaaa gaaaccccga aggtagctga
     3601 ggaaaagtta gaagaaaatc ttttgacaga aacaactcag aaagaggagc tttctgaaaa
     3661 agccaatgag caagacaaga ttgaggtggt tcaacaagag gacgtttctt cagagggcgc
     3721 aggttcggtg gaaaatggtc ccgaagcagc ttcagtaaat gcacaggttg aggaagaaac
     3781 aggtgataat agtaatcatt catcagaaga taccaatgag tttgctgccg acaaaacaga
     3841 ccttaaggta agtgaactta gtcaatctac ggcatcagaa ccaaaagacc ttgttgacca
     3901 gccagttgtg gaacagtttc caacaaaaca agcccaagct gatgcctcaa atgattcggc
     3961 gaatgaagaa gcagtagaca cttcaaaaga gcaatccagt agccaacagg ttatggaaga
     4021 ctactaccgt cgtaaggctg ctcttgaaaa aagccttcaa gaaaaagcag cggcaacggt
     4081 tccagtgatg ccagaagaag ttccacaaga aaatcaagcc tctacctcag cagaagcttc
     4141 tcaaaacaag gcaacctacg ataccattcc tgagacagat caagaaaaat acaagcgtag
     4201 cttgaaaaaa acgcggactg gcttctcagc tcgtctgaac agtttctttg ccaatttcag
     4261 gcgagtggat gaagaattct ttgaagactt ggaagaaatg ctgatcttat cagatgtcgg
     4321 tgtacatgtg gcgacaactt tgacagagga gttgcgttat gaagccaaac tggaaaatgc
     4381 gaaaaaacca gatgccttga aacgtgtgat tgtggaaaaa ttggtagata tctatgaaaa
     4441 agacgggcgt tataatgaag ccattaatta tcaagatggc ttgacggtta tgctttttgt
     4501 aggtgttaac ggggttggta aaacgacttc aatcgggaag ctggcttacc gttacaaaca
     4561 agaaggtaaa aaagtcatgt tggtggcagc agataccttc cgagcagggg ctgtggctca
     4621 gctagtcgaa tggggacgcc gagtagatgt gccagtcatt actggccctg aaaaggctga
     4681 tccagcgtct gttgtttttg acgggatgga aaaagctgtg gcaaaagaag tagatattct
     4741 tctgattgat acggctggtc gcctgcagaa caaagaaaat ctcatggcag agctggaaaa
     4801 aatgggtcgt atcattaagc gcgttctacc agatgcccct catgaaacgc ttttggcctt
     4861 agatgcgtca acaggtcaaa atgcgcttag ccaagccaag gaattttcaa aaataacacc
     4921 tttaacaggc ttaattttaa cgaaaattga tgggactgca aaaggtgggg tggtcctagc
     4981 catccgtcaa gaattagaca ttccagtcaa gttcatcgga tttggtgaaa aagtggatga
     5041 tattggagaa ttccactcag aagactttat gaaaggtctt ttagaaggta ttttataaga
     5101 aaaaaaacca gtcttgttca acagaacgag gctggttttt aagtaagtca gcttaggcaa
     5161 gtcctttttt ccctaaatgg taaaacatag caaagaaaaa gataaaatca agtagagcaa
     5221 aaatagtaaa gagaaggtag acagactgga caccgcttgt gaattctggt tgactatttg
     5281 ctatcagtga tgccatagca gttcctagag cgccagcaaa ttgctgcatc atctgaaaaa
     5341 tggccgtggc atcggcattt ttctcggcag gcaattctcg aatagccgtg gctaagctat
     5401 tattaaagcc catgttacgc ccaaacgtga ataaaatata taaaagagtg aatggtaaaa
     5461 gcataaaatg tcttgtttga agtgtcataa taatcaatga taaactaaat aaggcatttc
     5521 ctaaataaag cgaaagtctt gctccttttt gatcataaag tttaccaaaa gcaggtgcta
     5581 gtagagctcc gagtaaggta ccaggtagta ataccatacc agcttgagaa ctattcgcaa
     5641 tcttttccat gacaataaag tttggcgtta gaaaatttat gcctaaatta atcagctgga
     5701 agacaaaaaa gggaatcagg ccaaaggtta gagagggaat tttgagaatg cgaatatcaa
     5761 gaaagggttg cttagctgtg agattcttgt agaggaagag gataaggctg agaataaaaa
     5821 gccctaagta atacaaatta acacttccgt tttctagact agtaatagcc aataaggcag
     5881 aagttaagct aatcgatagt gcaataaatg ccaaccagtc aaagggtact ttttcgctta
     5941 ctggagaatc ttcgaggtag taataagcta gaatacctgc aatcagtggt acagggagta
     6001 tacagataaa aatccattgc caattaaaat ggctaatcat aaagccccca taagtaggcc
     6061 caaaagcagg tgctaagcta ataataagac cagcaaatcc cataaataga cctaccttat
     6121 gcattgggac acgctctaaa ataatattaa acatctgagg cattaccaga ccagtaccaa
     6181 tgccttgaaa aatgcgggct agcaacatga tcgcaaagga ctgggtcaga acggccaaga
     6241 tggtgccaaa tgtaaaaaga cctgtggcca taaaaaagag gggtctttcc cgcacatttt
     6301 ttttcagtgt agccgaagtg gtcatcataa tcgccactgc tagtaaataa atggtcgtca
     6361 tccattgcaa gctatttaag ggcaactgat agactgacat cagtgtcggg aaggtgacat
     6421 tcatactggt ttcagacaaa attcctgaaa aaccaatgag tgctatggcg ataatcgcca
     6481 taattgtttg ttttgaaacg gtttgttgat gatgtgacat gataactcct ttatctaatc
     6541 gattgttatt gtatcatata atcttaaaat tgtcagaaac tcttttttgc taaatcaata
     6601 gatacgaata acaatagttg ctctttcaga gaattttttg aaaacgcttg acagaatctt
     6661 aaagagatga taacattaaa gcgttctcag atgtgattac ttcaagtaag caggctagag
     6721 acataacaat cagaatatta aaggatgtta tcacagtttt gtgagcaggg acctattcga
     6781 aaagtgcttt ttagcgagct tttggatagg tcatttttta ttgtgatact aaaggactag
     6841 gacttttatg ttgattaaac gcgttttaaa ccataatgcg gcgatttcga ccaaccatca
     6901 aggattagat attttattga tgggaaaagg gattgccttt ggaaaaaaag ttggagatag
     6961 tattgagctt aatgctatcg aaacatcatt tgttttaaaa aatagtgaca atatgaatcg
     7021 cttcacagag ttatttatta cggtgccaca agaagttgtt gcttgctcag agagaattat
     7081 caatctaggt aaaattaaat taggcaaaac cttagatgaa attctctata ttaatttaac
     7141 ggatcatatt cactctgcta ttgaaaggca tgagcaagga atgttgattc ataacccact
     7201 acggtgggag attcaacgct attatcctga tgaatatagt ctaggggtaa aagccttaga
     7261 gctaattgag cgtaatctag gagtcactct agctattgat gaagcagctt ttattgccat
     7321 gcattttgtc aatgctagtt tggacactcc ttttaaggag ccacatcgcc tgacggagat
     7381 tgtttcttat attgagcaaa aaatcaaaac agacttcaaa acagaattgg atgacacatc
     7441 catcgattat taccgcttta tgacccatat taaattattt gctcaacgtg tgttatcaga
     7501 gatgtcttac gatgatgatg atgcagaact gcttatagtg gtgaaaacga aataccctaa
     7561 agaatatcgg tgcgtcttag atattagtga ggagataaaa aagcggtaca actatcacct
     7621 taattcaagc gaacttttgt atttgacggt tcatgttaaa cgtttagtga aacatttaaa
     7681 ggagacttaa tatgacgtat caagaaactg ccaaggctat tttagcggcg gttggtggca
     7741 aaacaaatat tcaacgtgtg acacactgtg taacacgtct acgcttagta ctgaaaaatg
     7801 atgagaaagt taaagatcag caagtcaaat ccatttcaaa tgtgattggc gtgatgcgca
     7861 aaaacggcca gtaccaaatt atcttgggca atgatgtcaa taattattac caagcctttt
     7921 taagcctagg gcattttgac aatcaagatg aagatcattc ttcaaaagcg aaaggaagta
     7981 tccttgagcg cttgattgaa accattgctg gtgtgattac accgctgatt ccagcccttc
     8041 ttggtggtgg aatgctcaaa gtggtgggga tcttgcttcc tatgcttggc ttagcgagtg
     8101 cggactctca aaccgttgcc tttattaact tctttggcga tgcggcttat tattttatgc
     8161 ctgtcatgat tgcctattca gcagcggcgc gctttaaggt gacacctgtt ttagcagcga
     8221 cgatcgcagg gattttattg catcctgcct ttgttgcaat ggtagcagaa ggtaaaccat
     8281 tggctttatt tggagcacca gtcactcctg ctagctatgg ctcatctgtt attcccattt
     8341 tgatgatggt ttacttgatg caatacattg aaaaatgggt caatcgcttg gtgccaagcg
     8401 tgatgaaaag cttcttacag ccaaccttaa tcattttgat ttctggcttt ttggctttag
     8461 tggttgtagg tcctcttggt gtgattattg gtcaagggtt atctaatacc atgctcgcta
     8521 tttatcatgt ggctccgtgg ctagcacttg ccattttggg agcgattatg ccgcttgttg
     8581 tcatgacggg gatgcactgg gcttttgcac caatcttttt ggccgcttcg gtcgcaacac
     8641 cagatgtctt gattttacca gcaatgttgg cttctaattt ggctcaagga gccgcatctt
     8701 tggctgttgc ctttaaaaca aagcagaaac aaacacgtca agttgccctt gccgcgggga
     8761 tttcagcttt gctggcaggt atcacggaac cggcacttta tggggtcaca ctgaaattta
     8821 aaaagccact ctatgcagcc atgatttcag gtggtctggt tggagccttt attggttttg
     8881 tcaatattgc ttcttatacc tttgtcgtac cttctattat tggtttacca cagtacatca
     8941 acccatcagg cggagctaat tttacaaatg ctttgattgc cggagctgcg acgattgtgt
     9001 tagccttcag tttgacttgg ttcatgggaa ttgatgaaga atccccagag caagtgagtg
     9061 ttgcagcaga tatgtcacaa gttaagagcg gcttgtcaac caaacaaacg ttgtatgctc
     9121 caatgacagg tgagatgctt tttctatcag aagttcctga tgaaaccttt tcttccaagt
     9181 tattaggaga aggatttgcc atattaccaa gcgaaggtga ggtctatgcc ccctttgatg
     9241 gtgaagtcat cactttcttt ccaaccaagc atgctgttgc cttaaaaaac acacgtggtg
     9301 tggaagtctt gattcatgtc ggtattgata cggttgagtt aaaagggcaa ggtttcgagc
     9361 agttagtgtc tgttggcgat ttcgtgaaac gaggccaggc acttctaaaa atggatattg
     9421 atttcattac ttcaaaaggc tattcactca tcagtcctgt ggtcgtgacc aattcggctg
     9481 agcagttgga gattattatt caagatgaca aaaaaatagt gactaaagag gacgctttgt
     9541 tagtcatttt ataacctaaa aggagaggga gaatgggaat ttttccaaaa gatttcttat
     9601 ggggcggcgc agtagctgcc aaccaagtgg aaggagcatt tgaggcagac gcaaaaggtt
     9661 tgtccgtaca agatgttcta ccaaatggtg gactaggaga atggacagat agtcctacca
     9721 gcgataattt aactttagag gcgattgatt tttaccaccg ctacaaagaa gacattgctc
     9781 tttttgccga gatgggcttt aaagtctttc gaacgtctat tgcttggtct cgtatttttc
     9841 caaatggtga cgatgaccag cccaatgaag caggtttgca attttatgat gacttattcg
     9901 atgaattgtt gaactatggt atcgaaccct tagtgacctt gtcgcattat gagacacctc
     9961 tgcatttggc caaagcctat aatggttgga cagatagacg tctgattggc ttttttgagc
    10021 gctttgctca gacagtcatg gaacgctata aggacaaagt caaatactgg ttgaccttca
    10081 acgaagtcaa ctcgatttta cacatgccct ttaccagtgg tggcatcatg acagaaaaag
    10141 aaaagctgag cctgcaagat ttataccaag ctattcatca tgaattggta gcatctgcta
    10201 gtgtcacaaa gctagcgcat gaaatcaatc ctgatgttaa agttgggtgt atgattttgg
    10261 cgatgcctgc ttacccaatg acctctgatc ctcgtgatat acttgctgcg catgcatttg
    10321 aaaatcttaa tctcttattt tcagatattc atgtgagagg caagtatcct tcatatatta
    10381 agtcgtattt taaagaaaat gggattgaga ttgtttttga agacggagac aaggaattat
    10441 tagcggaaca tacggttgat tttctatcct ttagctatta catgagtgtc acacaagccc
    10501 acaatcctga agcttacaca tctggtcagg gaaatattct aggtggtttg tccaatccat
    10561 atttagaatc ttcggagtgg ggttggcaga tagacccaat tggtctgcga ttggtcttga
    10621 atcagtacta tgaccgctac cagattcctt tgtttattgt tgaaaatggc ttaggtgcaa
    10681 aggatcagct ggtgcaaacc gcagatggca gcatgacggt tcacgatgat tatcgcattg
    10741 actacatgag tcagcatctc gtacaagttg ccaaagcgat tgaagatggc gttgaggtca
    10801 tgggttacac tgcttggggc tgtattgatt gtgtctctat gtcaacagcc caattaagta
    10861 agcgctacgg ttttatttat gttgaccgaa atgatgatgg tactggtcaa ctcacacgct
    10921 ataagaaaaa atcatttgat tggtaccgac aagtcattca aaccaatgga cgttaccttg
    10981 aggataattt ttcctaaata ggtcttgttt aagatggtga ctctgttcct atgctaactg
    11041 ataaaacgca aaaagcattt gttttgataa aaaacaagtg cttttttatt agtctttaaa
    11101 aaacgcggta aacataagga ttattaggtt ctttggtttg atgaacctca gcaagttgaa
    11161 ggacaggtag atgttgttct aagtttttat catattccat atgaaggcga cctttaactg
    11221 ttagccatgt attgttaggg tagctggttt gattacctgt ggtcaaaagt ccgtacactc
    11281 ctgagtcggc aatacagtga ataatgccga agcggaaaag gaattggtag ttgtcatggc
    11341 ccggttcgtt ataaacaaag ccagtgtatt ggatgtcacg atcaagaaac tcatctgggt
    11401 agagatagat caattccatc acttccatat aattttcagt ggtaatggtg accggtttct
    11461 tgcctttata tttatggagt tcttgacgca tctccttttg gtaggcagac tttgtaaagt
    11521 aaaggctggt atcaggtttg aggtattgaa tggtggtacc gtcatcactg actcccgttt
    11581 tagaggctcc cgcagccaat gggaaagtat agcctttagc agaaaccgtg gtggaatcta
    11641 gggttactgt tggcacaagg agaccaatca aaacaggaaa gaccaagata aaaggactgg
    11701 tcagccttgc gatttttcct gttaaatggc tatgtacctt aatgtttttc atccaggtat
    11761 agagttgaac aagagctaaa atgaaagata aaaccatgga aatataagca aggtaagaat
    11821 accgaacatt gatgtactga tccagtttgc cagacagctg gagatacatg gttagttcaa
    11881 aatagccaga taaaattaaa aagcgaatca ttagagcacc cccacaagta aacaataaac
    11941 ggcaatcata agaacggata cgccgataaa ttggacaatg aagcgtcctt taaaagcctt
    12001 taccatcatc ataaggtttt taatatccac cataggacca attaaaagaa aggcaagaac
    12061 tggagccact ccaaaggtgg ataataagga agctccgata aaggcatcgg cttcactaca
    12121 taatgataga atgaaagcca tcagcatcat gatcaaaatg gctgtcaagg gattgtgccc
    12181 aatcgttgtc aacacacgag ttgggacata tatttgcatg gcagaggcaa tcaaggttcc
    12241 aaatacgaga tacctaccgg tgtcgaagaa ttcatcaata gcatgtacca gtgctagata
    12301 aatgcgtttt ggcaagcttt catgagaata atcatgaaaa tgcacgggtt gagcattatc
    12361 cttgagaatg ttatcatcta caataaaagc taacattacc ccaagtgtaa tggctactag
    12421 tgcagcaccg accaagcgaa gtatcagaaa gcgcaaagaa ttcccaaaag cagaataagt
    12481 agcgaacaaa acaataggat taataatagg cgctgttgcc agaaaaggga cggccgtata
    12541 actaggcacc tttttttcca aaaagcggtt gatgatggga ataatcccgc attcacaaga
    12601 aggaaaaaca aatccaacaa aggttccaaa cagaatgcgc aagcattttt gcttaggtag
    12661 gtatttttga accaattctg gcgttacaaa gacttcaata caaccagata aaatagttcc
    12721 taggagcaca aaaggaagag cttcaatgat aatggacata aaaatcgcaa accattgcaa
    12781 gacactggga ggaaggttgg aaaagagtga cattcaccat atcctttcta gatgataaaa
    12841 gaggaggctg aaacacgaat gttcagcctc gactgatacg ttttgttagc ctgatcgcta
    12901 aaaatctctc aactcagttt aaacgaatgg acgatatgtt aagagaatga gacaaactag
    12961 ctatggtttg cttggttttg tttgttctgt cgttttttct ttggtcgcta gtttatcatc
    13021 agtattgact tccaaaggcc tatcaaaact agggattgat gcaaagcctg ctagcatttt
    13081 ttcaagttca tcattttttt tatgggtaat tgccatatct atgaccttct ttcattcaag
    13141 ataagtctat tatactatca tttgagaaca atgccaaaac ggaagtgttg tttacttctt
    13201 tcttttttaa aattaggtat tgttatttag ggtttcgtca gttatttact aaaagtagct
    13261 ttttttgatt ctttacgcaa ctttatatta ggacaatgtt tcgctatcat tttttgcatt
    13321 aatatggttt gtgttataat agcaatatta tggaaaataa caataatcac aacattgcag
    13381 aggctttgtc agttagtctc catcaaattg aacaggtgct tgccctaaca gcacaaggca
    13441 ataccattcc ctttattgca cgttaccgta aagaagtaac aggaaatcta gatgaagtgg
    13501 tgattaagtc cattattgat atggataagt ctctcactac cctgaatgag cgcaaagcaa
    13561 ccattctcgc taaaattgag gaacagggaa aactgacaga tcagttacga acgagtattg
    13621 aagcaaccga aaaactcgct gatttggaag agttgtacct gccttataag gaaaaacgcc
    13681 gtacaaaagc gacgattgcg cgtgaagcag gcttgttccc attagcgcgt ttgattttac
    13741 aaaatgcgca aaaccttgaa acagcagcag aaccctttgt cacagaagga tttgccagcc
    13801 cacaagaagc tctagcagga gctgtggaca tccttgtgga ggccatgtca gaagatgcta
    13861 aactacgctc ttggacctac aatgagattt ggcagtacag ccgcttagta tcaacgctta
    13921 aagatgagca gttggatgag aaaaaggttt tccaaatcta ttacaatttt tctgaccaag
    13981 tgtctaacat gcaaggatat cgtactttag cccttaaccg tggcgaaaag ttaggcatct
    14041 taaaagtgtc ttttgagcat aacttggaga aaatgcaacg ctttttcagt gtgcggttca
    14101 aagaaaccaa cccttatatt gaagaggtca tcaatcagac catcaaaaag aaaattgttc
    14161 cagctatgga aagacgggtt cgttcagaac tcagtgatgc cgcagaagat ggggctatcc
    14221 acctcttttc tgaaaatctc cgtcatcttt tgttagtgtc tcctttaaaa gggaaaatgg
    14281 ttcttgggtt tgaccctgcc tttcgaacag gtgcaaaatt ggctatcgtt gatcagactg
    14341 gaaaattgtt gactactcag gttatttatc ccgtagcacc agctagccaa acgaagattc
    14401 aagcagctaa agaaacactg actcagctca ttgagactta ccagattgat attattgcca
    14461 ttggaaacgg aacagccagt cgggaaagtg aagcctttgt agcagacgtt ttgaaagatt
    14521 tcccaaatac gtcctatgtc atcgttaacg aaagcggtgc ttctgtttat tcagcgtcag
    14581 agttagcccg ccatgagttt ccagacctca cagtggagaa gcgctctgct atttctattg
    14641 ctcgtcgctt gcaagaccca ctcgcagaat tggttaaaat tgatcctaaa tctattggcg
    14701 tcggtcagta ccagcacgat gtgagtcaga aaaagttgag tgagaatctt ggttttgttg
    14761 tggatactgt ggttaaccaa gttggtgtta atgtgaacac ggctagtcca tctttattag
    14821 cacatgtgtc aggtttgaac aaaacgattt cagaaaatat tgtcaaatac cgcgaagaaa
    14881 atggagcact gacatcacgc gctgacatca aaaaagtgcc gcgtttagga gctaaggcgt
    14941 ttgagcaagc agcaggtttt ctcagaattc caggagccaa aaatatttta gacaacacag
    15001 gagttcaccc cgagtcctac ccagcagtca aagaactctt taaggtactt ggtattcagg
    15061 acttggacga cgctgccaag gcaactttag cagcagttca agttccccaa atggcagaaa
    15121 cattggctat tgggcaggaa acccttaaag atatcattgc tgatctcctt aaaccaggtc
    15181 gtgaccttcg tgatgacttt gaagcaccaa tcttacgtca agatatcctt gatttgaaag
    15241 atttggaaat tggccagaag cttgaaggaa ctgtgagaaa cgtcgttgac tttggtgctt
    15301 ttgtagacat tggtgttcat gaagatgggc ttattcacat ttctgaaatg agcaaaacct
    15361 ttgtcaacca ccctagtcaa gttgtttcag tgggtgactt ggtaactgtt tgggtctcta
    15421 agattgactt ggaccgccac aaggtcaatc tcagcctatt gccaccgcgt gacactcact
    15481 aattatgtcc aagaagtttc cctggctgat tttggcaaac catttcacca caaggcttat
    15541 tggaacaagc gcctaaaaac aactggcggt cgcttttttc caaaagatgg tcatttggat
    15601 tttaacccta ggatgcttga ggagcacggt gagcttattt ttcggaaaat tgttcgccat
    15661 gagctctgcc attaccatct ttattttgaa ggaagagggt accatcataa agatagggat
    15721 ttcaaagatc tgctagctca agtaaacggg cttcgttatg tcccaactag ctctaaatca
    15781 aagactaatc atcattatag ttgccaaacc tgtgggcagg tttatcaacg aaaaaggcgt
    15841 atcaatctgg ctaaatatgt ttgtggaaat tgccacggga aacttatgga aaaaaatcag
    15901 tcgtaagact ggttttattt tgcgaaaaac aatgtaaact agtatcatag aatgaggagg
    15961 taaagaaacg tggaaactaa attctataaa cagcgaaaaa atcgcttggt tgcaggtgtc
    16021 attgctggtt tagcagataa gtatggttgg gatcttgctc tagcgcgtgt cttagcggct
    16081 cttcttattt acggaactgg ttttggagtt ttgctttata ttttgttggc catcttctta
    16141 ccttacaagg aagacttgct tgaagaacgt tatggccgag gtcctcgtcg tcgtaaagac
    16201 gctgatgtct taaatgaaga agaagatggc tggttctggt aaacaatcag ttaaaatgat
    16261 aattcatcac gtaagagcaa ccatgtgatt aactgatgat aataaaatgt tttgatatgt
    16321 aagtaagcga ggggtcttat cagctatgat gaggcttttt gctttttgac ttagtaagat
    16381 gatacctttt taaataccat aataagcact aattgctcaa tgatactagt aaagtatttg
    16441 tttgccattg tcttattttt gactcatatc tctaaatgat aaaacctagt ttttaataga
    16501 gctaagacca tttttggtgt cgttgttctc atttagtagt gaaaaaatga gtatttgcaa
    16561 gggtctaacg gtcgatttgt gttataatat tgaaaaagga gaaattatga ccgttactgt
    16621 aaagatgcta gtgcaaaaag ttaaacttga tgtggtttat gctacagata acttgctgtc
    16681 aaaagagata acaacatcag atatttcacg tccaggtctt gaaatgactg gttattttga
    16741 ttattatgct ccagaaaggt tgcagctgtt tggaatgaaa gagtggtctt atcttacaca
    16801 gatgacttct cacaaccgtt attctgtttt aaaagagatg ttcaaaaagg acactcctgc
    16861 agtggttgtc tctagaaatt tagccattcc aaaagaaatg gttcaagctg caaaagaaga
    16921 aggtatttca cttttaagta gccgtgtttc aactagccgc ttagctggag aaatgtctta
    16981 ttttcttgac gcttctttag ctgaacgtac tagtgttcac ggcgttttaa tggatattta
    17041 tggtatgggg gttcttatcc aaggggattc tggaattggt aaaagtgaga ctggtttaga
    17101 attagttaag cgagggcatc gtttggttgc agatgaccgt gtggatgtct atgccaaaga
    17161 tgaagaaacc ctttggggag agccagcaga gatcttgcgc cacttgcttg aaattcgtgg
    17221 ggtaggtatt attgatgtga tgtcgcttta tggagctagt gctgtgaagg actcatcaca
    17281 agttcaattg gccatttacc ttgaaaactt tgaggctggt aaagtttttg atcgtctcgg
    17341 aaacggtaat gaggagataa ctttctcagg agtgagaatc cctcgtattc gtattccggt
    17401 aaaaacaggt cgaaatgttt cagtggttat tgaagccgct gcgatgaatc atcgggctaa
    17461 agaaatgggc tttgatgcta caaaaacttt tgaagatcgt ttaactcaac taatcaccaa
    17521 aaatgaggtg agtcaatgat caatcccatt gccttaaaat gtggtccact agccatccac
    17581 tggtatgctc tatgtatctt gtcaggactt gttttggcgg tttacttagc atcaaaagaa
    17641 gcacccaaaa aaggaatatc atctgacgct attttcgatt ttattctgat tgcttttcca
    17701 cttgctattg ttggtgcaag aatttactat gttatttttg aatggtctta ttatgtcaag
    17761 catttagatg aaatcatagc catttggaat ggcggtattg ctatttatgg cggtctcatt
    17821 acaggagctc ttgtactttt ggcttactgt tataacaagg tgctcaatcc cattcatttt
    17881 ttagatattg ccgcaccaag tgtcatggtc gctcaagcta tcggtcgctg gggaaatttt
    17941 atcaaccaag aagcctatgg taaagctgtg agccagttaa attacttacc aagctttatt
    18001 caaaagcaaa tgtttataga gggaagttac cgcattccga cctttctcta tgaatcactt
    18061 tggaatcttt tgggatttgt cattattatg atgtggcgtc gtaagccaaa aagtctatta
    18121 gatggagaaa tctttgcatt ttacttaatt tggtatggta gtggaagact agtcattgaa
    18181 ggtatgcgaa cagatagtct tatgttttta ggtattcgca tctcccaata tgtgtctgcc
    18241 ttattaatta ttattggctt aatctttgtt ataaaaagac gtcgtcaaaa aggaatttct
    18301 tattatcaag aataaggata gaaaaaggag aaacttatgg acttagttgg aatctcactg
    18361 atgattattg cactcggatt tgttgctttg gtgattttct tgattattgt attaaagaaa
    18421 gtctcagaaa caattgatga agccaaaaaa acgatttctg tattgacaag tgatgttaat
    18481 gtgacacttc atcaaactaa tgatatttta gcaaaagcta atatccttgt tgaagatgtt
    18541 aatggtaaag tagcaaccat cgatccactg tttgttgcta ttgctgattt gtcagaaagt
    18601 ctttcagatt taaatagtca agcaaggcat tttgggcaaa aagcaactaa tgctacaggt
    18661 aatgtttcaa aagctggaaa attagcatta gttggtaaag tagcctcaaa agtatttgga
    18721 aaaaaaggag aaaagcatga ataaaagttt caaaaactta gtgattggag ctgtttcagg
    18781 agtagcggca gcttactttt tatcaacaga aaaaggcaag gctttgaaaa atcgtgctga
    18841 aaaagcttat caagcctaca aagaaagtcc agatgattac caccaatttg ctaaagaaaa
    18901 aggtagtgaa tacagccatt tagcacgtga tactttctat gatgtgaaag ataagttggc
    18961 ttctggtgac ttaactaaag aggacatgtt agacttgctc aaagataaaa caacagcttt
    19021 cgtgcaaaaa acaaaagaaa cccttgccga agttgaagcg aaagaaaagc aagatgatgt
    19081 tatcattgat ttaaatgaag aagacatcat cattgactat actgaacaag atgagcctgt
    19141 tagtgataca ctagataaac actaactaaa acgacctaaa acatagaaga agaaaagatt
    19201 gaaggatctg atcactagtc ccgatggtta gttttaggct aactgaagaa ggggggtatt
    19261 gtggcttcaa tctttttttg tttttgacac aaaaaaaaga gtcacgtaaa acggactctg
    19321 gaagttacat cagtaatgat ttgataacct tggctaataa ggaagtaatg cctaagctga
    19381 tgatgcttgc aaataagaaa gcaaacattg gcgctaaatt cattacgagc aggacaaatg
    19441 caaataaaac ggtaataagg ctgaaactag cgatacgagc aaagaagact aattctttta
    19501 gctttgcact ttggccattg aactcttgaa aatctcgaaa tttactttga gattcttggt
    19561 aagaatcatc ctgttcaacc gaattatgct tctttaacgg tgcaggcata gtcctctcct
    19621 ttccttaaca tactaagtaa ttctatcata aaagtctctt cttgtcatct ttttttaaaa
    19681 gatttgaaaa gatttactaa tgacaagtga cttatcatgc tataacagta aggggactag
    19741 cttaacgttt gggcttttgc aagaaatatg cttaaggtct taaaattttt ggtataataa
    19801 agcttatatg gaaaaaataa ttattacagc cacagctgaa agcattgagc aagtcaaagc
    19861 tttattagct gctggtgttg atcgtattta tgttggtgaa gccaattacg ggttacgtct
    19921 tccccataac ttttcttatg atgaattgag acaaattgcc aaactagtgc atgatgcagg
    19981 caaagagttg acagtagctt gcaatgcttt gatgcatcaa gatatgatgg atcagatcaa
    20041 acccttttta gacttaatga tagagattgc tgttgattat cttgtggttg gtgatgcagg
    20101 tgtgttttat gtcaataaaa gggatggcta taactttaaa ctgatttatg atacctctgt
    20161 ttttgtgact tctagccgtc aggtaaattt ctgggggcaa catggtgctg ttgaaagtgt
    20221 tttagcaagg gaaatcccat cagcagagtt attcacctta gctgaaaatt tggaatttcc
    20281 agcagaggtg ctagtttacg gcgcctcggt gattcatcac tcgaaacgtc ccttgctaga
    20341 aaattattac cattttacaa agattgatga tgaggttagt cgtgaacgag gacttttctt
    20401 ggcagaacca ggagatgcta gcagccatta ctccatttat gaagacaatc atggcactca
    20461 tattttcatt aataacgata tcgacatgat gtctaaattg ggtgagctgt atgcgcatgg
    20521 cttaactcat tggaagctag atggcattta ctgtccaggt gatgattttg tcgctattac
    20581 taaattgttt attcaggcta aaaccttatt agaagctggg caatttaccc aagaagaagc
    20641 tgaaaagctt gatcaagcag tgcatgccca tcatccagct ggccgtggat tagatactgg
    20701 cttttatgag ttcgatccaa aaaccgttaa ataagtaatc cttgtcttga gtgagtttac
    20761 tatttcaggg caagacaggg ttatgacacc attacacagg taatctacct atcttgaaaa
    20821 tagaaagaat atcacatgtc acatatgaaa aaacgtcccg aggtcttatc acctgctgga
    20881 acacttgaaa aattaaaagt tgcgattgac tatggcgcag atgctgtttt tgttggaggg
    20941 caggcctatg gcctaagaag ccgcgctggt aacttctcta tggaagaatt gcaagaaggc
    21001 attgattatg cacatgcgcg tggagctaag gtctatgttg ctgctaacat ggttacccac
    21061 gaagggaacg aaattggtgc gggcgagtgg tttcgtcaac tgcgtgatat ggggcttgat
    21121 gcggtcattg tttcagatcc agccttgatt gttatttgtt caacagaagc cccaggtttg
    21181 gaaattcatt tgtcaacgca agcttcatct accaattacg agacctttga attttggaaa
    21241 gccatgggct tgacacgtgt tgttttagct cgcgaggtta atatggccga gttagcagaa
    21301 atccgtaagc ggacagatgt ggaaattgaa gcctttgtcc atggagccat gtgtatttct
    21361 tattcaggcc gctgtgtttt gtcaaaccac atgagtcacc gtgatgccaa taggggcggc
    21421 tgctcacagt cttgccgctg gaagtatgat ttgtatgaca tgccatttgg aggagagcgc
    21481 cgctccttaa aaggggaaat tccagaagac tattctatgt cctctgttga catgtgtatg
    21541 attgaccata ttcctgacct gattgaaaat ggggttgata gcttaaaaat tgaaggccga
    21601 atgaaatcta tccactacgt ctcaaccgta accaactgtt acaaggcggc tgtagatgct
    21661 tacatggaaa gcccagaagc tttttatgct atcaaagagg aattgattga cgagttgtgg
    21721 aaggttgccc agcgcgagtt ggctacaggt ttttattatg gtatcccaac tgaaaatgaa
    21781 caattatttg gtgctcgccg caaaattcca caatataaat ttgtcggaga agtagttgcc
    21841 tttgactcag ctagcatgac agcgaccatt cgtcagcgta atgtcatcat ggaaggcgat
    21901 cggattgaat gttatggacc aggtttccgt cattttgaaa cggttgttaa ggacttacat
    21961 gatgcggatg gccaaaagat tgaccgtgcc ccaaatccaa tggaactctt aaccatctct
    22021 ttaccgagag aagttaagcc aggggatatg attagggctt gcaaggaagg ccttgtcaac
    22081 ctctatcaaa aagatggcac cagtaaaact gttagaacat agtaacaaga aatactagag
    22141 gccctctttt tccaaatggt atacgaccat agttgggaaa agagagcctg ttttttttga
    22201 aaattggata tcacaatagc ttattttctc aaaacttgat agaataatag gagaatgagt
    22261 cataaaaaaa gtgaaaaaaa gatgattttt tccactttaa tttcgaatag tatagatgag
    22321 gaggaaaact aatggcagaa tttacattca acattgagga acatttactc accttatcag
    22381 aaaatgataa aggttggacg aaagaattga accgcgtcag ctttaatggc gcagaagcga
    22441 aatgggatat ccgcacgtgg agtccagacc atacgaaaat ggggaaaggc attactctta
    22501 ctaatgaaga atttaaaacc atcttagacg cttttcgtaa gtaatgttac cttaatcgaa
    22561 acataatccc tcgttcgccc ttgtcctctt aacctggtga tgacatcgct aggttaagag
    22621 gacaggggcg aatagaagga ttattttttt gaattgaggc gatcagcaag gccgacaagt
    22681 accaaaatta agcctagccc aagaaaaatc catttggcag ctaaaaggcc ataaagcaag
    22741 aagatagtag cactaatgta ggagatatat tctttggtca tcttgttaag tcactttcta
    22801 taagctactg ttaataaacc agtcttcatg gtaatgaaga ctggttagag ataataaaac
    22861 gttatgttat attgtcactt atcggtttta agatatgaat acaaatctta aaaaagttaa
    22921 ggtttcatcg ttgggaataa aagcacatct ctgattgttg ttgtgtttgt taataacatg
    22981 caaagacgat caatcccgat tccgagtcca ccggttggcg gcataccata ttcaagagct
    23041 tcaacgaagt cgtagtcaat gccagttgct tcgtcatcac caagttcttt agcttgtgct
    23101 tgtgcttcaa aacgagataa ttggtcaatg ggatcattta actctgtgaa agcattggcg
    23161 tattctttgg tcatgatgaa aagttcgaag cggtcggtga agcgggtatc ttctggattc
    23221 ttcttagcca aaggtgatac ttcaacagga tgaccaaaga caaaggttgg ttggaccaag
    23281 gtttcttcaa cgaattcttc gaagaaagca ttgatgatat gtccgacact tgtgaagtgt
    23341 ttttcgagtg ggacctgttt ttctttggca agtgcaatag cttcttcgac agtcatttca
    23401 ggccagaaat caacacctgt cacttccttg atagcatcta ccatgtggac acgtttgaaa
    23461 ggttcattga tacggatttc agttccttga taatcaatag gcccatcacc tttaacagct
    23521 ttagcagcat gttggataat gccttctgtc aggttcatga tatcaagata gtctgcatag
    23581 gcttggtaaa cctcaataga agtgaactca ggattatgag tagcatccat tccctcgtta
    23641 cggaagatac gaccaatttc atagacacgt tccataccac caacaatcaa acgtttcaaa
    23701 tgaagttctg ttgcgatacg aagcaccata tcaatgtttt gagcattatg gtgagtaacg
    23761 aaaggacgag cagcagcacc accagcctcg ttatgaagca caggcgtttc tacttctagg
    23821 aaatcaagac cgtctaaata acgacggatt tctgaaatca ttttggaacg ggtcacgaag
    23881 cggtcaaagc tttcacgatt tgaaattaaa tcaaggtgac gcttacggta aatggtttca
    23941 atatcagtta atccgtggaa tttttcagga agaggacgaa gtgatttaga aaggtgagtc
    24001 aacttggttg ccttgattga caactcgccc atgtctgtac gcatgacttc gccttcaacg
    24061 ccgataaagt cgcctaaatc ggcttttttg aagatttcat agttgtcttc gccaacagag
    24121 tccttgcgaa cataaagttg gatttggcct tcacggtctt gaagatgagc aaaaccaacc
    24181 ttacctttgc cacgttttgt catcaagcgg ccagcaacaa tagctgtttc gtttaactca
    24241 tgtaattctt cttttgtttt atcagcatac ttttctttta attcagcaga attggctgtg
    24301 cggtcaaagc gtttaccgaa aggatcaatt ccttgttcag ccagagccat catcttttca
    24361 cgacggacaa tctgttggtc atttaattct tcgatgtgtt gattagacat attttcctcc
    24421 aatacgattt cctttccatt gtatcataaa agaggctaga atacacctat atttcgttgg
    24481 aaaatgcgac tgcagcgggg cacttttttg aaaataaaat tgaaaagcct aatggaaaat
    24541 agtataatga ttgggatatt aaactagaaa agaaaagaga ctcatgatta ctgctattgt
    24601 ttttgatgtg gatgacacaa tttacgacca gcaagcgcct tatcgaatcg ccatggaaaa
    24661 gtgcttccca gattttgata tgtcagtaat gaatcaagct tatattcgct ttcgtcatta
    24721 ttcagatgtt ggattcccaa gagtaatggc aggggagtgg accacagaat atttccgatt
    24781 ctggcgttgc aaggaaacac tgcttgagtt tggctatcga gaaatcgacg aagctgcggg
    24841 agtacatttt caagaagtct atgagcacga acttgagaac attaccatgt tagatgagat
    24901 gcgcatgacc cttgactttt taaaatcaaa gaatgttcct atggggatta ttaccaatgg
    24961 gccaacagaa catcagctga aaaaagttcg taaattaggg ctttatgatt atattgatcc
    25021 taagcatgtc attgtgagcc aagcaacagg ttttcaaaaa ccagaaaaag aaatttttaa
    25081 cctggcagca gaacaatttg acatgaatcc acaaaccaca ctatatgttg gggactctta
    25141 tgacaatgat atcatgggtg cctttaatgg tggttggcat tccatgtggt ttaatcatag
    25201 aggtcgtcag ctaaaaccag gaactaagcc tgtttatgat gtggctattg ataactttga
    25261 acagctcttt ggtgccgtta aagtactctt tgatttacct gataataaat ttatttttga
    25321 tattaatgac aagaaaaatc cgattttaca gatgggaatc aataatggct tgatgatggc
    25381 agcagagcgt ttacttgaaa gtaacatgag cattgacaaa gtggttatct tgcttcgatt
    25441 aaccaagcag caagaaaaag tgctacgttt aaaatatgcc aaatagatga aaccataata
    25501 gcccactaga taataataaa aaaagctagc aaagtgagtg tcttgtcagt aagaaatcaa
    25561 tgtgttagct ttttttatta ttgtctagtg gttttacacc tctataaaag atttatcatt
    25621 ccaaattaaa cagtcataag ttaaataatc cttagtttca agaatagtaa gactggtatt
    25681 atctaagcca cctttagcga gtaaaagagc aggttcaaaa cctaataatg agcgaatagt
    25741 tgctgttaag ttagcgccgt gtcctacaat aagaacgttt tgataatgtt tatctttaaa
    25801 cgattggata agatggcaga cacgttgagt cgtttggtaa attgattcag cctcgaactg
    25861 atctggtttg aactgggcta gattctcccg aaaagccagc atttgttggg gatatatagc
    25921 tgccattgtt gctattttag ccccttccaa tttacctaat ctccactccc gaagctggtc
    25981 ggtgtggtaa agttttggct gttggtcaaa agcatctaag ataatagctg ctgttgccat
    26041 agctctttga aggtcgctag tataaacggc atcaaaatcc accttagcta actctttgcc
    26101 aagtaagtga atttcgtctt ttgcctcttc aagtaatggc gaatcgcccc cagcgccttg
    26161 aaagcgacct tctaaattcc aaagggtttt cccatgtctt acaaaatata atttcattta
    26221 agcctccttt tagagataac tgatgcgagc gctaaaaata aaaaagtaac cagaaaaagt
    26281 ttaggcataa ccaagcaaaa ataataccta tagtgaaaag ataaagtaaa aaagcgtagt
    26341 ataagtgtat aatagctagg ttctgattaa agtgtaaagc ccatattaga tttaagacaa
    26401 atcgacctga atttgtcgtg atatcaagcc tttacaaaag aaaaagatta acaggatcaa
    26461 tgttaaagca gttgagatca aaataatact aaacaaagac tagcttaatc aaggcgaaaa
    26521 acttaaaaaa gaaaccagat aaagttggca gtagtgttca ttttctcttc tttaaatcag
    26581 caaagcttgc gccaacgaaa tcagctagag cctggctaga aatctttata gaacgcccaa
    26641 cttcaccagc tgaaacgatt atttgacctt tttccagtgc agtttggtca ataaaaatag
    26701 gatatgaatg tttttgacga atcccaacag gattattggc accgtgaata tagcctgttg
    26761 tcttttgcaa gtctttttga ggaaccatgg acactttttt gttacctgat acttttgcca
    26821 attgtttttc tgataagtgt tctgttaatg gaataatgcc aattagagga cctgtttgat
    26881 caccagtcag agcaagtgtc ttgtaaatat ctgatggttg aagatcatct ggaaagtcgc
    26941 cttctaaagc attaagcttg agtccttgat gagcaatatt agccttatcg aggatttgtt
    27001 ctactaaggt ttttttgagt tttgtttttt tagccatatc aagtccttac ctttatctaa
    27061 acataaaaaa
//
LOCUS       DAUGHY010000022        25662 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.022, whole genome shotgun sequence.
ACCESSION   DAUGHY010000022 DAUGHY010000000
VERSION     DAUGHY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25662
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.022"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..608
                     /gene="speC"
                     /locus_tag="VH752_000920"
     CDS             <1..608
                     /gene="speC"
                     /locus_tag="VH752_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeC"
                     /protein_id="HEQ8289282.1"
                     /translation="NVKSDLLYAYTITPYDYKDCRVNFSTTHTLNIDTQKYRGKDYYI
                     SSEMSYEASQKFKRDDHVDVFGLFYILNSHTGEYIYGGITPAQNNKVNHKLLGNLFIS
                     GESQQNLNNKIILEKDIVTFQEIDFKIRKYLMDNYKIYDATSPYVSGRIEIGTKDGKH
                     EQIDLFDSPNEGTRSDIFAKYKDNRIINMKNFSHFDIYLEK"
     gene            complement(676..1890)
                     /locus_tag="VH752_000921"
     CDS             complement(676..1890)
                     /locus_tag="VH752_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEQ8289283.1"
                     /translation="MTFLDKIKQGCLDGWAKYKILPSLTAAQAILESGWGKHAPHNAL
                     FGIKADASWTGKSFNTKTQEEYQAGVITDIVDRFRAYDSWDESIADHGQFLVDNPRYQ
                     SVIGEADYKKACHAIKDAGYATASGYAELLIQIIEENNLQKWDKEAIVRKEKQMINSQ
                     CREVIEFFINLANAGMGVDKDSFAGWQCADVPCYAAKHWFDVDLWGNAIDLLDSAAAV
                     GWEVHRMPTDANPLTGAFFVQSVPYHQFGHTGIVIEDSDGYTMRTVRTVEQNIDGNPD
                     ALYVGAPARFNTRDFTGVIGWFYPPYQGDTVTQPVSTEPQTSETIVETAKTGTFTLDV
                     AEINIRRWPSLASEVVGSYKQGDTVSFDNEGYANGYYWISYVGGSGMRNYLGIGQTDK
                     DGNRISLWGKLN"
     gene            complement(2006..2233)
                     /locus_tag="VH752_000922"
     CDS             complement(2006..2233)
                     /locus_tag="VH752_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEQ8289284.1"
                     /translation="MINLKLRLQNKVTLMAILGAIFLLAQQLGIKLPSNIADIANTAV
                     TLLVLLGVVTDPTTKGLSDSEQALTYHEPKK"
     gene            complement(2230..2505)
                     /locus_tag="VH752_000923"
     CDS             complement(2230..2505)
                     /locus_tag="VH752_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289285.1"
                     /translation="MATELIFGVGGFILAIVTTYNIFNAKSIKHATDITLLQSEVEHL
                     KIVTRQNARRLEEHAEQNKTLITMTEQIKNLTDDVKDLKDMMRGEIK"
     gene            complement(2515..3132)
                     /locus_tag="VH752_000924"
     CDS             complement(2515..3132)
                     /locus_tag="VH752_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEQ8289286.1"
                     /translation="MRNWKVTGKYPQFDSTGAVASTHIIITAEDGSVISQLVKQDLIS
                     TNDTEIIKATLEEFKKSEYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQKMINLQTIHMLMSGGKIDSDIYKGMLELIEPAKKGEYQAYDVFTVVDSTKEED
                     GEAGEGNLVFVHVNEAFEYDKQTLEELESEAKVTVIKYADLVKQD"
     gene            complement(3135..3566)
                     /locus_tag="VH752_000925"
     CDS             complement(3135..3566)
                     /locus_tag="VH752_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEQ8289287.1"
                     /translation="MDLTLKNKELNTLYRVLDKIKITNMRANRGRAKLLAKVVDKINE
                     YAKDETDLIDMYAAKDKDDKFVIDEHKNIKLADPAKLDELNDLLNELADEEIVIKGGE
                     YSKRFIDFLNFLEECEDEFTSSEIILIDNILEQFEESKKGE"
     gene            complement(3578..5464)
                     /locus_tag="VH752_000926"
     CDS             complement(3578..5464)
                     /locus_tag="VH752_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEQ8289288.1"
                     /translation="MSRDPTLTLDESNLVIGKDGRVHYTFTAEDDNPKVRLASKCLGT
                     AHFNQLMIERGDQATSYVAPVVVEGTGNPTGLFKDLKEISLELTDTANSQLWSKIKLT
                     NRGMLQEYYDGKIKTEIVNSARGVATRISEDTDKKLALINDTIDGIRREYRDADRRLS
                     SSYQSGINGLKAQLANDKIGLQAEIQATAQGLSQKYDNELRQLSAKITTTSSGTTEAY
                     ESKLAGLRAEFTRSNQGMRIELESQISGLRAVQQSTASQISQEIRDRTGAVSRVQQDL
                     ESYQRRLQDAEDNYSSLTHTVRGLQSDVGSPTGKIQSRLTQLAGQIEQRVTRDGVMSI
                     ISGAGDSIKLAIQKAGGINAKMSGNEIISAINLNSYGVTIAGKHIALDGNTTVNGTFT
                     TKIAEAIKIRADQIIAGTIDAARIRVINLNASSIVGLDANFIKAKIGYAITDLLEGKV
                     IKARNGAMLIDLSSAKMDFNSNATINFNSRDNALVRRDDTHTAFVHFSNATPKGYTGS
                     ALYAAIGITSSGDGVNSASSGRFAGMRCFRHATGYNHTAAVDQTEIYGDSVLIADDFS
                     INRGFKFRPDKMTKVLDMNDLYAAVVALGRCWKHLANVGWNTVHGNFTNAVTNELNNH
                     INKI"
     gene            complement(5477..6487)
                     /locus_tag="VH752_000927"
     CDS             complement(5477..6487)
                     /locus_tag="VH752_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
                     /protein_id="HEQ8289289.1"
                     /translation="MTETIPLRVQFKRMTAEEWTRSDVILLESEIGFETDTGYAKFGD
                     GKNQFSKLKYLNKPDLGAFAQKEETNSKITKLESSKADKNAVYLKAESKIELDKKLNL
                     KGGVMTGQLQFKPNKSGIKPSSSVGGAINIDMSKSEGAGVVVYSNNDTSDGPLMSLRT
                     GKETFNQSALFVDYSGKTNAVNIAMRQPTTPNFSSALNITSGNENGSAMQIRGVEKAL
                     GTLKITHENPNVEANYDENAAALSIDIVKKQKGGKGTAAQGIYINSTSGTTGKLLRIR
                     NLGDDKFYVKHDGGFYAKKTSQIDGNLKLKNPTADDHAATKAYVDSEVKKLKALLMDK
                     QV"
     gene            complement(6484..8628)
                     /locus_tag="VH752_000928"
     CDS             complement(6484..8628)
                     /locus_tag="VH752_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ8289290.1"
                     /translation="MIPVLYEAKETKFRTFGLGEIADAYEVKATRERNGNYSLYIKYP
                     LDGVFASVFKEEMKIKSDAGRRTKWQTFEINRVLRNSKDHIEIFARHISMRTQDIALK
                     PFVNGASVGAESALEIWKKNLVGDDKFDVKSDILTLGSFSWEADKIGNARGALGGVAG
                     SILDVYGGEYEFDNRTIILRKQMGRKAPTVLEYGRNIVSVEEERLLDGNYTSIYPYVR
                     YTPQPKPQEEAPGKPHVGEHKQPEEQLVTLPEFILDGQYLSLYAQRRIQMVDLSSHFN
                     DDKNKKEPTVEEIRKLAQKYLKDNNVGAPKVSIEVDYIDLSQTLDYQDFRVMEEVELC
                     DIVPLYYPKFGITTESEKVVEIVYDVYTDSNHTIKLGTIGQSISKSLTGGVSERINAL
                     ENNQKVITNNQKQFELNLPKYLNDINGKRVWYEKPDDNIEHKIGDYWFEKNGKYQRTW
                     IWDGNQWVKVLDTEDLNPNQRAFDEAMAEIEKAKKAQEEINQRTDKELEEFRATLKNL
                     ALPEEAIKKITEAIKVDDIPSIKQSFDDLKNKVSETSETSRLNAEILGNNGKTRYNKN
                     LLVGDPNRTKTYDEDYIEVEANDGGFKRGETYTISFSQTCELLQKVAITLTQANNKGV
                     KLVLTPTKAKTDAQTFEVTKDKQSIEVYPLSYTGVLTGDWYKPKQIDLNASDTQELAL
                     EMAYKEVVDGKDAAITGIWSDSPQIILDGGKK"
     gene            complement(8625..9332)
                     /locus_tag="VH752_000929"
     CDS             complement(8625..9332)
                     /locus_tag="VH752_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEQ8289291.1"
                     /translation="MRYIEFNGTKSNDLGLLLERERSIKSTNNDVDLIEVAGRDGVLI
                     KDNERLKAVEQDFPFSLVGDVTANQQKISEWLHVKGWHDLALSWDKDYIYRASVVNLF
                     EIDEILKQFGRLKINFLIHPIKYLKTGKQEVSLVNGSTLQNPGNVQAKPILKIKGIGN
                     GVLTINGFKTGLENVQGELVIDMERHLVYKDVLSAWDNIVRTERHRMPLFDVGQNKIS
                     WTGSFTITAVPNWGVKV"
     gene            complement(9332..13255)
                     /locus_tag="VH752_000930"
     CDS             complement(9332..13255)
                     /locus_tag="VH752_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEQ8289292.1"
                     /translation="MGNIGDLVATATLDISPFMSNTRNLKTYMKGLDNSLKAVEKSFQ
                     GHGGRIKGLKAVYAETGSALKGYQELLKTQSQKYSDLKKEIGDVNNATAEQKQKLIGA
                     KSAMLETAAQVAELQNRLRALATETSVFTRFGKAAERIGGKMKSFGDSVAGVGAAFTR
                     GVTAPIVAGAGYAIKAAVDYESAFAGVKKTVDETATVSYAKLSQGIRQMAKELPASAV
                     EIAHVAEAAGQLGVKTGDILSFSRTMIDLGESTNLSAEEAATSIAKIANITGLASSEY
                     SRFGSAVVALGNNFATTERDIVAMTNRIAASGKLAGLTNQEMLALATAMSSVGIEAEA
                     GGTAMTQSLSAIERAVASGGDNLNKFAQIANMSSADFARAWKEKPIVALQEFIKGLGQ
                     LDKKGESATKVLDELGLSGIRQSNMLKSLGLASETLGKALGISNKAWKENTALTDEAN
                     KRYETTESKLKMLKNEVNDVAIEFGGPLVDALRNGLEAGKPIIQMAADLAKQFNSLDK
                     EQQQQIIKWGLIAAAAGPALSILGKGIGVIGGTIQAIGKMSKGIGALSGWLRTFKAGA
                     VAASVGAEAATASMGSLTGAVALLSNPVTWGVLLGGAAVIGIGLIADSMYKAQKRTEE
                     WGTAVSATEATALSNFKKKVDETNTSLQMFEAGAGSVKKVTEAFDDLVGSIEKLAQSK
                     LDKNINLAKKLGLSEETINALKSKTESVVNNVKSMNTQIKAIMEKHNGDMSQLSSAEK
                     ELVLRNQREMIIAQLDLMKFSASEKKALTAALNNELDALNARQLEKVSENTVKMLDKE
                     NSAYKTKKAELKEILKQFGSDTSKLSAEELAARQEVLNRLTELNMQHNLKTKALNDQY
                     LAIQRERVQRLKESGKSQEEIHRGISQMASDMAQKLGISYDDAYRKMAYYTEKSGETL
                     KVLSRNTANATAEVAAANAQWDSLFTSDNPQQSLNELLSTAEGWNSFEIMVKNADVEP
                     TGRAALAEMLVAGGQWQNMTLEQKKLVVDGQQAMIEIFDSKELLAQWQVLTPEEKVLL
                     AKNLTQEPTMSAQQALDSVKQTVPADVNATDKTAGDTQSAQSKIDNVKQKAPADMKAS
                     DKTGPDVASANRAVNSPKQNSPAVIRAQDNASGVAENVIWSLARIPRSVTTTITTFVR
                     KIFGHEKGTDFHPGGLAVVNDQKGALYRELVTLPTGESFIPTGRNVILPLPRGSKVLK
                     ASRTKQLFPHYANGIGFDDTRIASLTTRLKSVQDKGTVVVNADPQLAELIKLLKDRDD
                     RNVTNNYTLNATNSSSSEDMFSQENMRRLVRELAYYTKGEEGRLA"
     gene            complement(13268..13417)
                     /locus_tag="VH752_000931"
     CDS             complement(13268..13417)
                     /locus_tag="VH752_000931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289293.1"
                     /translation="MKNGYTILDIKKMRLSDIELMVQALEIETVEKEEVIETTLDKAF
                     PFLFG"
     gene            complement(13465..13791)
                     /locus_tag="VH752_000932"
     CDS             complement(13465..13791)
                     /locus_tag="VH752_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289294.1"
                     /translation="MYEITLKKGGVDKEFKKDFINVEDNLLAVEHQVRQSAVFSDEKR
                     RLDSKAHRKLNESYLQMFVDMYAGQFTVDDLKQSDMTVLNTLNDLYIAALGGEQEEDE
                     SEKKEQ"
     gene            complement(13844..14452)
                     /locus_tag="VH752_000933"
     CDS             complement(13844..14452)
                     /locus_tag="VH752_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ8289295.1"
                     /translation="MGKVKFGLRDFYYGVLDGNDKVTKAESIKHLPGMKSAKLDITNE
                     LVTVPADDGPYVVLSGGISETKLEIELLDLTSDARKDFFGITVEKGVEKYNKNLTPND
                     IVCLFRTSDENGKAIWIALLKGKFNLPGMEAQTKEGAPDPKPDTTTGNFVARGPKEEV
                     LLVGREGNSGFNLNEFLKCVFAGCGDAEIKKIPIAEQASTVM"
     gene            complement(14468..14893)
                     /locus_tag="VH752_000934"
     CDS             complement(14468..14893)
                     /locus_tag="VH752_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289296.1"
                     /translation="MIAETAAYKLLSNDKTLNELLDRLRGGTFKNGFKQGIFTYDIPD
                     NPVNLRKVELAPFMRINTTLDGPFDYADDEILCNEQRITINFWCKTASEADQINKCID
                     NILKQGGFERYTANEKPRYKDRDIDLLMNVRKYRYFDFY"
     gene            complement(14890..15267)
                     /locus_tag="VH752_000935"
     CDS             complement(14890..15267)
                     /locus_tag="VH752_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEQ8289297.1"
                     /translation="MTKGLDEILANLTKLEVKASKTAKAAVTKVAKEFEKTLKSNTPV
                     YEIETDERLQEDTVISGFKGSNVGIVSKEIGYGKATGWRAHYPNDGTIYQRGQDFKER
                     TINQMTPRARQIYAEKVKEGLGL"
     gene            complement(15264..15611)
                     /locus_tag="VH752_000936"
     CDS             complement(15264..15611)
                     /locus_tag="VH752_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEQ8289298.1"
                     /translation="MITRKMNVRITIFSQSGGQNEDGEVVSAIRKDLYTCWAEVLKTQ
                     LRDFNYQSKFQNASNLPTNKDTKVFLIRYNPELSIDNTMFVEFDKRIYKIDKIESDES
                     GKDMIMISGVSMS"
     gene            complement(15608..15910)
                     /locus_tag="VH752_000937"
     CDS             complement(15608..15910)
                     /locus_tag="VH752_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEQ8289299.1"
                     /translation="MAVSKELLDSVKLYCKIDFDFEDDIIKEMIESAQEQICFAIEEG
                     STAGTFKESAKFALAVKKQVKEEYDHRGLSADSFRYPLANGVLNIIHQLRLRGDDS"
     gene            complement(16055..17242)
                     /locus_tag="VH752_000938"
     CDS             complement(16055..17242)
                     /locus_tag="VH752_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEQ8289300.1"
                     /translation="MFEEKIKEIKATIASLNQAIATKTAEVKNALESDDLETARSIKA
                     EVEQAKADLVEAENDLKLYEASIEKGGAENTGGKEVPQETKTYRESVNEFIRSKGTVT
                     NEALRFEGKDEVLIPLNQTTPIDPKTDGVKKTDVKPVSSEEILYTPAREVKTVVDLKQ
                     FTSIHPAKKASGKWPVLQCATEKMVSVEELEKNPKLAKPKFKDVEWKVETYRGAIPLS
                     QESIDDADVDLVGIVAETIGQMKVNTTNDAIAKVLKTFEPKADVKTLDEIKKILNVDL
                     DPAYNVSFIVSQSFYQSMDTLKDENGRYLLQDSITSVTGKVFLGKPVFVLADEVLGKN
                     KAFVGDFKRGVLFADRKDLGLRWVDNDIYGQYLQAVLRFGVAKVDDKAGYYVTFTPEQ
                     LPS"
     gene            complement(17267..17932)
                     /locus_tag="VH752_000939"
     CDS             complement(17267..17932)
                     /locus_tag="VH752_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEQ8289301.1"
                     /translation="MPKRINLKGPLIANNSQEVYDYYGMEATSAKSIINQLPEDSSDI
                     ILEVNSNGGLVTVGSEVYTALRNYKGKVTAEITGMAASAASVAVMGADKVVMSPTAQM
                     MVHKALFNWVAGNSDDLDKASNALKSSDKAIVNAYVAKTGLSEDEIMNLMRNETFMSA
                     QDAVEKGFADEVMSFEAVASIDNQMLPQAVIDDYYANRSKRKQEISNMLLEIEKEEIL
                     QGL"
     gene            complement(17910..19130)
                     /locus_tag="VH752_000940"
     CDS             complement(17910..19130)
                     /locus_tag="VH752_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ8289302.1"
                     /translation="MSFFRPLGSSKVSYDDYISSVLAGDVSQKYLGVSALKNSDILTA
                     TSIIAGDIARFPLVKKDVNGDIIHDEDINYLLNVKSTKNASARTWKFAMAVNAILTGN
                     SFSRILRDPKTNQALQFQFYRPSETTVEETDSHEIIYTFTDTLTAKQVKCFAHDVVHW
                     KFFSHDTILGRSPLLSLGDEIDLQTGGINTLIKFFKDGFSSGILTMKGAQLSGDARQR
                     ARQEFEKMREGSVGGSPLVFDSTMEYTPLEIDTNVLQLITSNNFSTAQIAKALRVPSY
                     KLGVNSPNQSVAQLMEDYVTNDLPFYFDAITSELGLKTLNDKDRRLYRIEFDTRSVTG
                     RNVDEIVKLVNNQILTPNQGLIELGKQKSTDPNMDRYQSSLNYVFLDKKEEYQDKVGI
                     KGKGGEVNAKEDKS"
     gene            complement(19164..19388)
                     /locus_tag="VH752_000941"
     CDS             complement(19164..19388)
                     /locus_tag="VH752_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289303.1"
                     /translation="MNKRIKKKRKLETAIVLLIKEVAELRSIVSANAKATNNELAAVK
                     SAILDNQVAIKSIGDEVGHIKQNYKRKWRK"
     gene            complement(19381..19551)
                     /locus_tag="VH752_000942"
     CDS             complement(19381..19551)
                     /locus_tag="VH752_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289304.1"
                     /translation="MIDFILKNIHTLILLAGLGLLMYGLFLFGDKVGFIASGLILIVL
                     AIYVDSVGGKRE"
     gene            complement(19548..21302)
                     /locus_tag="VH752_000943"
     CDS             complement(19548..21302)
                     /locus_tag="VH752_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEQ8289305.1"
                     /translation="MKVEYDYSAISDIYKDDAFYYAKMVVDEQIKSSKKVFRACLRHL
                     NDLKKIDGDNFKFIYLPEKAADPINFIEILPDVKTGKPYPLAMFQKFIIGNLYGWRKK
                     TDHSLRRFRKAMISVARKNGKTILIAGILLYEFLFGHNPSMSRQLFCTANDRTQAKIA
                     WDMAKKQLSSLRAKDADVRKATKIVRDELKNLHDESYIRALSRDTGAVDGFEPYVGVL
                     DEFAASKTNEMLELLESGQGQLDNPFILIISTAGMDLNVPMHTIEYPYITKILDGEIT
                     DEGYFGYVAEQDNEEEIKDESNWIKSNPILEVEALHDKLMDYLRTRRRVSLETGEINK
                     VLIKNFNMWRQSSEESYIDKTTWELARIDKPDTNKRRVWLGVDVGRVSDLFAITPVVM
                     MDDFWYIDSFSFVATKYGLTAKEKRDGVSYSNLECQGYCEITTLESGVIDDERVLEKI
                     EEMVYSNDWEINGICFDPYQFGTLLTMIEKRHPEWPLIEVSQTTMVLNMPTKQFRDDL
                     KKGKIKHSGNPLLTMAINNAYIKTDNNGMRIDKNKNSNKIDPLDAALDGYAVCYLEPF
                     DGSGYWTNEKIIGGESLF"
     gene            complement(21306..21536)
                     /locus_tag="VH752_000944"
     CDS             complement(21306..21536)
                     /locus_tag="VH752_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289306.1"
                     /translation="MERVKEFSVKLLENGKFPIVEINGEKINGITKLIVKYDSDDAQN
                     NPLDNGFLVEYISEEGGRYYMQTIGQSFRIGL"
     gene            complement(21539..22006)
                     /locus_tag="VH752_000945"
     CDS             complement(21539..22006)
                     /locus_tag="VH752_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEQ8289307.1"
                     /translation="MGRNLKLVETTKKHLTKEEKIVRETAQNKASDGLKKLQKTPPEH
                     FNNVAKYEYRRIIEDLQNLPLRNLDRGLLELYCTWYAIYKETSRKLDEVGYFTNDPDK
                     GIIPSPLILTLEKATANIRSSASQLGLTVDSRMKMFIPKEEEKPKSIFDKFGG"
     gene            complement(22177..22515)
                     /locus_tag="VH752_000946"
     CDS             complement(22177..22515)
                     /locus_tag="VH752_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEQ8289308.1"
                     /translation="MRPQKLTIAGGRRTTVDYDDRSAEYRDYNRNRWKYDKQAKQFYN
                     SRIWRETSKQVLLQNDYICAMCGGEATMTDHIVSVKQDWNKRLDWNNLQASCKACNDS
                     KAIRERRKNN"
     gene            22751..22936
                     /locus_tag="VH752_000947"
     CDS             22751..22936
                     /locus_tag="VH752_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEQ8289309.1"
                     /translation="MPMTPKQMIKLLKKNGFYEVSQNGSHKKFRDNNGHQTIVPMHNK
                     DLGKGLEDTILKQAGLK"
     gene            22988..23365
                     /locus_tag="VH752_000948"
     CDS             22988..23365
                     /locus_tag="VH752_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEQ8289310.1"
                     /translation="MLIYPATFTQDTDYIMVTFPDVPEAITQGEDFQEAYEMAVEVLG
                     FALEDYTDYPKASAVSDLKEQYPDSDIALIGIDMVAYMKKYHSKKVRKNVTIPEWLND
                     AAEDKNLNFSQVLTEALELKLQA"
     gene            complement(23492..24385)
                     /locus_tag="VH752_000949"
     CDS             complement(23492..24385)
                     /locus_tag="VH752_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEQ8289311.1"
                     /translation="MLKLDEKKIRKGKPVGLPYQGSKKKISKKIVEIIKQNFDADKPI
                     YDVFGGGGAITAECVLNGLEVHYNDLDNDITDMFQRVISQDREWIKTLIISRDEFNKI
                     RQKEPKSVDDNLKLLVNSFGNELSSYLYGADWSDTKYDLAVEIINKHDVFSGYKQTET
                     YKKADKPYDEGELEKNKKLTQLEQLQQLGRLQQLGRLEQLEPTNYSYEAFSDIEDAIF
                     YLDPPYENTTQKSYKGDFNSQAFYDWAFGMSKNNIVLISSYDISDERFECVYEFKTAR
                     STMQGGGAGKRTEKLFMAVIT"
     gene            complement(24968..25405)
                     /locus_tag="VH752_000950"
     CDS             complement(24968..25405)
                     /locus_tag="VH752_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEQ8289312.1"
                     /translation="MGNIPTTKANNFLEELKTIPHLIETLERDANLMSRSLVKSPQWS
                     DMKVSGGVKQSQEDKNIKMLHMVGYYSDQIEQLKDRRQEMANLIVQSMGICESHVLLT
                     TYLDCDGDYERARERLNIGNRNKYFMFVRRGKESLELILKNTN"
BASE COUNT         6850 a         5744 c         4430 g         8638 t
ORIGIN      
        1 cgaatgttaa aagtgattta ctttatgcat acactataac tccttatgat tataaagatt
       61 gcagggtaaa tttttcaacg acacacacat taaacattga tactcaaaaa tatagaggga
      121 aagactatta tattagttcc gaaatgtctt atgaggcctc tcaaaaattt aaacgagatg
      181 atcatgtaga tgtttttgga ttattttata ttcttaattc tcacaccggt gagtacatct
      241 atggaggaat tacgcctgct caaaataata aagtaaatca taaattattg ggaaatctat
      301 ttatttcggg agaatctcaa cagaacttaa ataacaagat tattctagaa aaggatatcg
      361 taactttcca ggaaattgac tttaaaatca gaaaatacct tatggataat tataaaattt
      421 atgacgctac ttctccttat gtaagcggca gaatcgaaat tggcacaaaa gatgggaaac
      481 atgagcaaat agacttattt gactcaccaa atgaagggac tagatcagat atttttgcaa
      541 aatataaaga taatagaatt atcaatatga agaactttag tcatttcgat atttatcttg
      601 aaaaataatt catcatacac aaaaaaccgc ccagaataat ctgagcggtt ttgtcttatc
      661 tcggagcttt acctcctaat ttaatttacc ccaaaggctg atgcgattcc catctttatc
      721 agtctgtcca atacctaggt agttacgcat acctgagccg ccaacatagc taatccagta
      781 gtaaccgttg gcataaccct cattgtcaaa gctgacagta tcaccttgct tatagctacc
      841 tactacttca ctggctaggc ttggccagcg tctgatattg atctctgcaa catcaagggt
      901 aaaggtgcct gtttttgctg tctccacgat cgtttcagaa gtttgcggct cggtgctgac
      961 tggttgcgtg actgtatccc cttgatatgg tgggtaaaac caacctatca cgccagtaaa
     1021 gtcacgagtg ttaaaacgag ctggtgcacc gacatacaaa gcatcaggat tgccatcaat
     1081 gttttgctcg acagtgcgca cagtgcgcat ggtgtaaccg tcactatcct cgataacaat
     1141 tcccgtatgt ccaaattggt gatacggcac tgattggaca aaaaatgctc ccgtcagtgg
     1201 atttgcatct gtcggcatgc gatggacttc ccaacctacg gcagcagcgc tatctagtaa
     1261 gtcaatcgca tttccccaaa ggtcgacatc gaaccagtgc tttgctgcat aacaaggcac
     1321 atctgcacat tgccagcccg caaaactatc tttgtcaaca cccataccag cattggccaa
     1381 attaataaaa aattcaatga cttctcgaca ttgagaatta atcatttgct tctcctttct
     1441 gacaatagcc tctttatccc atttctgcaa gttattctcc tcaataattt ggataagtag
     1501 ctccgcatag ccactcgcag tcgcatagcc tgcatctttt atagcatgac aagctttttt
     1561 ataatcagcc tccccaataa cagactggta gcgtggatta tctactaaaa attgtccatg
     1621 gtcagcgata gattcatccc agctgtcata ggctctaaac ctgtcaacaa tgtcagtaat
     1681 aacaccagct tgatattcct cttgggtttt ggtgttaaac gacttaccgg tccagcttgc
     1741 gtctgcctta ataccaaaca aagcgttatg tggtgcatgt ttaccccacc cgctctctaa
     1801 gatagcttgt gctgcggtca aggatggcaa gattttgtac ttagcccagc catctaaaca
     1861 gccttgttta attttatcta aaaaggtcat ctgtcctcct tatctaaaaa cggataaaag
     1921 ataagagcaa ccacagataa tggcacatac agtattgcga ttgctataac taatgctaat
     1981 cgtgtgattg ctcgcatggc tcctcctatt ttttgggctc atggtaagtc aatgcttgtt
     2041 cgctgtcaga taagcctttt gtagtaggat ctgtgacaac gccaagcaac accaaaagcg
     2101 ttacagctgt gttggcaata tccgcgatgt ttgatggtaa tttaatacct aattgttgcg
     2161 ctaacaaaaa gatagctccc aaaatagcca tcaaggttac tttgttttgt agtcgtaatt
     2221 ttaaattgat catttgattt ctcctctcat catatctttt aaatctttaa catcatctgt
     2281 taaattttta atttgctctg tcattgtaat caacgtttta ttttgctcag cgtgctcttc
     2341 aagccgccga gcattttggc gtgtaacaat ttttaaatgc tctacctcag attgcaacaa
     2401 agtaatatct gtcgcatgct tgatggattt tgcattaaaa atattgtaag tcgtgacgat
     2461 agctaaaata aagccaccaa cgccaaatat caactctgtt gccataaacc acctctaatc
     2521 ttgtttaacc aaatcagcgt acttgataac tgttactttg gcttctgact ctagctcctc
     2581 taaggtttgt ttgtcatact caaatgcttc gttaacgtgt acgaagacta ggttcccttc
     2641 gcctgcttca ccgtcttcct ctttagtact gtcgaccacc gtaaagacat catacgcttg
     2701 atactcacct tttttggctg gctcgattag ctcaagcatg cctttataaa tgtcagaatc
     2761 aatcttgccg ccgctcatta acatgtggat cgtttgtaag ttaatcatct tttgcgtgcg
     2821 ctctgcggac accttagcta gtccagcagc tgtttgagca gttttggcag tcttagcggt
     2881 ttcctgtgag attttttcaa ggtcgtctac cttttgcacg gcttcgccca ttgcaatttc
     2941 aacgtattca gattttttaa attcttccaa agtagctttg ataatctctg tgtcattagt
     3001 cgagattaag tcttgcttaa caagttgaga gatgactgag ccatcctcag cagtgataat
     3061 aatatgtgtg cttgcgactg ctcctgtgct gtcaaattgt gggtattttc ctgtcacttt
     3121 ccaatttctc atggttattc tcctttttta ctttcttcaa attgttccaa aatgttgtcg
     3181 ataagaatga tttcagatga tgtaaattca tcttcacact cttctaaaaa gtttaaaaag
     3241 tcaataaatc gcttggagta ctcaccccct ttaatcacaa tttcttcatc tgctagctcg
     3301 ttgagtaggt cgttgagctc gtcgagttta gcggggtctg ctagcttgat gtttttgtgc
     3361 tcatcaatga caaatttgtc atctttatcc ttagctgcat acatatcaat taagtcggtc
     3421 tcatcttttg catactcatt gattttatcg actactttgg caagcagctt agcacggcca
     3481 cgattagcac gcatattagt gattttgatt ttgtctagta cacgatatag tgtattgagt
     3541 tctttatttt ttaatgttaa atccattgtt tctcctatta aattttgttg atatgattat
     3601 ttaactcgtt agtgaccgca tttgtaaaat tgccatgaac ggtattccag ccgacgttag
     3661 ctaagtgctt ccaacaacgg cctaaggcta ctacggccgc atacaagtca ttcatatcga
     3721 gtactttagt cattttgtcc ggcctaaatt taaagcctct attgatgcta aagtcatctg
     3781 caataagtac actatcacca taaatttctg tttggtcgac tgccgcagtg tggttgtagc
     3841 ctgtagcgtg cctaaagcaa cgcatacccg caaaacgtcc agatgacgca ctgttgaccc
     3901 catcaccaga tgaggtgata cctatggctg catagagtgc cgaacctgta taaccttttg
     3961 gtgtggcatt actaaaatgt acaaaagcag tgtgtgtgtc gtccctgcgg accagagcgt
     4021 tatcacgact gttaaagttg atggtcgcat tactattaaa atccatctta gccgagctaa
     4081 gatcaataag catagcgcca ttacgagctt tgatgacttt gccctcgagc aaatcagtga
     4141 tagcatagcc aattttagct ttgataaagt tagcgtctaa accaacgata ctacttgcgt
     4201 taaggttaat tactctaatc ctagcagcgt caatcgtgcc tgcaataatc tgatcagccc
     4261 taatcttgat agcctctgct atctttgtgg taaaggtgcc gttgacagtc gtattgccat
     4321 cgagagcgat gtgtttacct gcgattgtta ctccgtagga gttgaggtta attgctgaga
     4381 taatctcatt accagacatt ttggcattaa tgccgccagc cttttggata gctaatttaa
     4441 tgctgtctcc agcgccacta ataatactca tgacaccatc tctagtaacc cgctgctcaa
     4501 tttgtcctgc tagttgagta aggcgtgatt ggattttacc agtcggggac cccacatcac
     4561 tctgcaaacc tctaactgta tgggttaagc tactgtaatt atcttccgca tcctgcaaac
     4621 gacgctgata actctctagg tcttgctgca cacgactgac agcacctgtc ctgtctctaa
     4681 tctcttgtga gatttggcta gctgttgact gctgtacagc tcttagtccg ctgatttgag
     4741 actcgagctc tatcctcatg ccttgattac tacgagtaaa ctcagcacgt aagcccgcaa
     4801 gcttactctc gtaggcctct gtagtgccgc ttgaggttgt tgtgatctta gccgataact
     4861 gtcttagctc gttatcatac ttttgtgata atccttgagc ggttgcttgt atctcagctt
     4921 gtaaaccgat tttatcattg gctagttgag cttttaggcc gttaatgcca gactggtaac
     4981 ttgaggataa gcgcctatca gcatctcgat actcacgcct gataccatca atggtatcat
     5041 tgatgagcgc tagcttttta tcagtatcct cgctgatacg tgtagcgaca cctctggcgg
     5101 agttgactat ctcggtcttg atcttaccgt cgtagtattc ctgcaacata ccacggttag
     5161 tcagcttaat ttttgaccaa agctgggaat tagcagtatc tgtcagctct aagctaatct
     5221 ctttgaggtc tttaaatagt ccagtcggat tacctgtacc ctcaactact actggcgcaa
     5281 catagctagt agcttggtct cctcgctcaa tcatgagctg attaaaatgc gctgtgccta
     5341 gacacttgct agctagtctg acttttgggt tgtcgtcctc tgcggtaaat gtgtaatgca
     5401 cacgtccatc cttaccaata acgagatttg actcgtctaa tgtcaacgtt ggatctctac
     5461 tcatatttcc tcctctttac acttgcttat ccattaagag tgcttttaat tttttgactt
     5521 cactatcaac ataagcttta gttgccgcat gatcatccgc tgtgggattc ttgagtttca
     5581 ggttgccatc aatctgcgaa gttttcttgg cataaaaacc accgtcatgc ttgacgtaga
     5641 acttatcatc accaagattt ctaatcctaa gcaacttccc tgtcgttcct gaggttgagt
     5701 taatgtagat tccttgagca gcagtacctt ttccgccttt ctgtttttta acgatatcaa
     5761 tagataacgc tgcagcgttt tcatcgtaat ttgcctcaac gtttgggttt tcgtgtgtga
     5821 ttttgagcgt tcccaatgct ttttcgacgc ctctaatttg catcgcacta ccattttcat
     5881 tgccgctagt aatattaagc gcagaggaaa aattgggggt ggttggctga cgcatcgcaa
     5941 tattaacggc attagtctta ccgctgtaat cgacaaaaag cgctgattga ttgaaggtct
     6001 ctttacccgt ccgcaagctc attaacggcc catcactggt atcattgtta gaatagacaa
     6061 caacaccagc accttccgat ttagacatat caatgttaat cgctcctcct acggaagatg
     6121 agggtttaat accactttta ttaggtttaa actgtagttg acctgtcatg acgccgcctt
     6181 tgagatttaa tttcttgtca agctctattt ttgactctgc ttttaagtaa acagcgtttt
     6241 tatctgcttt gcttgattct aatttggtga ttttactatt agtttcttct ttttgtgcaa
     6301 acgcacctag atctggttta ttaaggtact taagcttact aaattggttt ttaccatcac
     6361 caaacttagc atatcctgta tcggtctcaa agcctatttc actctcaagt aagatgacat
     6421 cgctgcgagt ccattcttcg gcagtcatcc gcttaaattg gactcgtagc ggtatagttt
     6481 ctgtcatttt ttacctccgt ctaatataat ttgtgggctg tctgaccata tccctgtgat
     6541 tgcggcatcc ttaccatcca caacctcttt ataagccatc tctagagcca attcctgcgt
     6601 gtctgaggcg tttaaatcta tttgcttagg tttataccag tcaccagtta aaacgcctgt
     6661 ataacttaaa ggatatacct ctatagactg tttatcctta gtgacctcaa acgtctgtgc
     6721 atccgttttt gctttagttg gtgtcagcac taacttaact cccttgttgt tagcctgtgt
     6781 cagcgtgata gccacttttt ggagtagctc acaagtctgg ctaaagctaa tcgtgtacgt
     6841 ctcgccacgt ttaaaaccac catcgttggc ttctacctcg atgtaatcct catcataggt
     6901 tttggtgcgg ttagggtcgc caaccaataa atttttgtta tagcgggtct taccgttgtt
     6961 acctaaaatt tcggcgttta gacgggacgt ctcgctcgtt tcactgactt tgtttttgag
     7021 gtcatcaaag ctttgtttaa tagacgggat gtcatcaact ttgatagcct ctgtgatttt
     7081 tttaatcgct tcctctggta acgctaggtt tttgagggtg gctcggaatt cctctagctc
     7141 tttgtcagta cgctggttaa tttcttcctg cgcttttttg gctttttcga tttcagccat
     7201 tgcctcgtca aaggcccgtt ggttagggtt taaatcctct gtatctagta ctttgaccca
     7261 ttgattgcca tcccaaatcc atgtgcgctg atacttccca tttttctcaa accagtagtc
     7321 gcctatctta tgctcaatat tgtcatctgg tttttcgtac caaacgcgtt taccattgat
     7381 gtcattgagg tatttaggca gattgagttc aaattgtttt tggttgttag taattacctt
     7441 ttgattattt tccaacgcat taatacgttc agaaacacca ccagtcaaac ttttagagat
     7501 tgattgacca atcgtaccta atttgattgt gtgattgcta tctgtataga cgtcatagac
     7561 aatctcaact actttttcag actcagtcgt gatgccaaac tttggataat aaagcggcac
     7621 aatgtcgcaa agctcaacct cctccatgac cctaaaatct tgatagtcaa gcgtttgtga
     7681 caagtcgata taatcaacct caatgctgac tttaggggcg cctacgttat tatccttaag
     7741 gtatttctga gccaactttc taatttcttc gactgttggc tcttttttat ttttgtcatc
     7801 gttaaaatga cttgataaat caaccatttg gattctgcgc tgagcatata agctgagata
     7861 ttgaccatct aggataaatt caggcaatgt caccagctgt tcttcgggtt gtttatgctc
     7921 gcctacatgc ggcttaccag gggcttcctc ttgcggtttt ggttgtggcg tatatcttac
     7981 gtaaggatag atagaggtgt aattgccatc tagcaagcgc tcctcctcta cgctgacgat
     8041 attacggcca tactccaata ccgtgggagc tttacgcccc atttgcttgc gtaagatgat
     8101 tgtacggttg tcaaactcgt actcgccacc gtaaacatct aggatagagc cagcgacacc
     8161 tcctagagca ccacgggcat tgccgatttt atcagcttcc cagctaaagc tcccgagcgt
     8221 taggatgtcg cttttaacgt caaacttatc atcaccgaca aggtttttct tccagatttc
     8281 taaagctgat tcggctccta cgctcgcacc gtttacaaac ggttttaaag caatatcctg
     8341 tgtgcgcata gagatatgac gcgcaaaaat ctcgatgtgg tctttactat ttcgtagtac
     8401 ccgattaatc tcaaaagtct gccatttggt tctacgacca gcgtcagact taatcttcat
     8461 ttcctcttta aaaactgagg caaagacacc atctagcgga tatttgatgt acagtgagta
     8521 attaccattg cgctcacgag tggctttaac ctcataagca tccgcaatct caccgagacc
     8581 aaaagtccta aacttggttt ccttagcctc atacaaaact ggtatcatac tttaaccccc
     8641 cagtttggca ctgcggtaat tgtaaagcta ccagtccacg agattttatt ttgtccaacg
     8701 tcaaataacg gcatgcggtg gcgctctgtc cgcacgatat tatcccaagc cgacaagaca
     8761 tctttataga ccagatgcct ctccatatct atgacgagtt ctccttgcac attttccagc
     8821 cctgtcttaa agccattaat ggttaaaacc ccattgccta tacctttgat ttttaagata
     8881 ggtttagcct gaacattgcc gggattttgt agagtactac cattaacgag agacacctct
     8941 tgcttacccg tttttaagta tttgataggg tggattaaga agttgatttt cagtctgccg
     9001 aattgtttta gaatctcgtc tatctcaaaa agattgacaa cactagcccg atagatatag
     9061 tccttgtccc aagacaaagc taagtcatgc caacctttga cgtgcaacca ttcacttatt
     9121 ttttgttgat tggcagtcac gtcaccaacc agcgaaaaag gaaagtcttg ctcaaccgct
     9181 tttaagcgtt cgttgtcttt aataagtaca ccgtcacgac cagctacttc gattaaatca
     9241 acgtcattat ttgtcgactt aattgaccgc tcgcgttcta gcaacaaacc taaatcattg
     9301 cttttagttc cgttaaactc aatgtatctc actaagctaa cctcccttct tcaccttttg
     9361 tgtagtaagc taattctctg actagccgtc tcatgttttc ttgactaaac atatcttctg
     9421 aactactgct attagtagcg tttagtgtat agttgtttgt gacatttctg tcatctctgt
     9481 ctttaagcag cttaatcaac tcggcaagtt gtggatcagc gttaacgact acagttcctt
     9541 tatcttgcac agatttaaga cgagttgtta agctagcgat tcttgtatca tcaaaaccta
     9601 ttccatttgc atagtgcggg aataactgtt ttgttcggct ggcctttaaa acttttgacc
     9661 ctctcggtag agggaggata acgtttcgac cagttgggat aaatgattct ccagtcggta
     9721 aggtaactaa ctctctatat agcgcccctt tttggtcatt gaccacagct aacccgccag
     9781 ggtggaaatc agtccctttt tcgtgtccga aaatcttacg gacaaacgtt gtaatggttg
     9841 ttgtaacact tcttgggatt ctagccagtg accatataac attttctgca acgccgcttg
     9901 cgttatcttg tgctctaata acagctggac tattttgttt aggactattg actgccctat
     9961 tggcgcttgc aacatctggt ccagtcttat ccgatgcctt catatcggct ggcgctttct
    10021 gcttgacatt atcaatctta ctttgcgccg actgagtatc acctgctgtt ttatccgtag
    10081 cattcacatc agcaggtact gtttgtttaa cgctatcaag agcttgttga gcggacatag
    10141 ttggttcttg tgttaagttt ttcgctagta agactttttc ttctggtgtc aacacttgcc
    10201 attgcgctaa caactctttg ctatcaaaaa tttcaatcat ggcctgttgc ccatccacaa
    10261 ccaatttttt ctgttctaac gtcatgtttt gccattggcc accagctact agcatttcag
    10321 caagtgcggc tctccccgtc ggttcaacat cagcgttctt aaccatgatt tcaaagctat
    10381 tccaaccttc tgccgttgat aacaattcgt ttaaactttg ttgtggatta tcactcgtaa
    10441 acaaactgtc ccattgagca tttgctgctg ctacctctgc agtagcatta gcggtattac
    10501 gtgataaaac tttcaacgtt tcaccggatt tttcggtata gtaagccatt ttgcgataag
    10561 cgtcatcata gctaatgcca agcttctgag ccatatctga cgccatttga cttatgccgc
    10621 ggtggatttc ctcttgactt ttaccagatt cttttaaccg ttggactcgc tccctctgga
    10681 tagcaagata ctgatcattc aaagctttgg tttttaggtt gtgctgcata ttaagttcgg
    10741 taagtctatt caaaacttcc tgtctagcag ccaactcttc agcactcaat ttactagtgt
    10801 cgctgccaaa ttgcttcaaa atctctttta actctgcttt ttttgtttta tatgcagaat
    10861 tttctttatc gagcatctta acagtgtttt cagatacttt ttccaactgc cttgcattta
    10921 gcgcatctaa ctcgttattc aaagctgctg ttaaagcttt cttttctgat gcagaaaact
    10981 tcattaaatc aagttgagca ataatcattt ctctctgatt tcgcaaaaca agttcttttt
    11041 cagcgcttga caactggctc atgtcaccat tgtgcttctc catgattgct ttaatctggg
    11101 tgttcatgct tttaacattg ttaactactg attcagtttt ggatttcaaa gcatttatgg
    11161 tttcttctga caatcctaat tttttagcta agtttatatt cttatctaat tttgattgag
    11221 ctaacttttc aatacttccg accaaatcat caaaagcttc agtcactttc ttaacgctac
    11281 ctgcgcctgc ctcgaacatt tgcaaagaag tgttagtttc gtcaactttt ttcttaaagt
    11341 tacttagtgc agttgcttct gtcgcggaaa cagcggttcc ccactcttcc gtgcgttttt
    11401 gggctttata catgctatca gcaattaaac caataccaat aacagctgcg ccacctagca
    11461 aaacacccca cgttaccggg ttgcttaata aggcaaccgc gccagtcaaa ctgcccatag
    11521 aagctgtggc agcttcagct ccaacacttg ctgctactgc accggcttta aacgtgcgta
    11581 gccaaccaga taaagcacca atccctttgc tcatcttgcc gatagcttga atggttccgc
    11641 cgataacacc aataccctta cccaaaatag ataaagccgg cccagcggcg gctgcaataa
    11701 gtccccactt gataatttgc tgttgttgct ctttgtcgag cgagttaaat tgtttagcta
    11761 agtcagccgc catttggatg attggcttcc ctgcttcgag cccgtttctc agagcgtcaa
    11821 ccaaaggacc accaaattct atggctacat cattgacttc gtttttaagc attttcagct
    11881 tagactctgt tgtctcgtaa cgtttgttag cttcgtcagt caacgccgtg ttttctttcc
    11941 aagccttatt ggaaattcca agtgccttgc ctaatgtttc agatgctaaa cctaatgatt
    12001 tcaacatgtt agactggcga ataccgctta atcctaactc atcaagtact tttgtggcac
    12061 tttcgccttt tttatcaagt tgaccaagcc ccttaataaa ctcttgcaat gcgacaattg
    12121 gcttttcttt ccacgctcta gcaaaatcgg ctgaggacat gttagctatc tgagcaaatt
    12181 tatttaaatt atcgcctcca gatgcgactg cacgttcaat agctgataat gattgagtca
    12241 ttgctgtacc accagcctcc gcctctatac caacgcttga cattgctgta gctaaagcta
    12301 acatctcctg attagttaat cccgcaagct taccagatgc tgctatacga ttggtcattg
    12361 caacaatgtc tctttcagtt gtcgcaaagt tattccctaa cgcaacgaca gcacttccaa
    12421 aacgcgaata ctcagatgat gctagacctg taatatttgc aattttagca atagacgtcg
    12481 ccgcttcctc tgcagataaa ttggtagatt ctcctaaatc aatcattgta cgagagaaac
    12541 taagaatatc tcctgtctta actcctaatt gacctgctgc ttctgcaacg tgagcgattt
    12601 caacagcact ggctggcaac tctttggcca tttgtctaat gccttgcgac aacttagcat
    12661 aggataccgt cgccgtttca tccacggttt tcttgacacc ggcaaaagca gattcataat
    12721 cgacagccgc tttaatagca taaccagctc ctgcaacaat cggagctgta actcctcttg
    12781 taaatgcagc acctacaccg gcgacagaat cgccgaatga cttcatcttc ccgccaattc
    12841 tttcagcagc tttaccaaat cttgtaaaga cgctagtttc agtggctaaa gctcgtaatc
    12901 tgttttgcaa ttccgccact tgcgctgctg tttctagcat ggctgattta gcgccaatta
    12961 atttttgttt ttgctcagca gtagcgttat taacatcacc tatctctttt tttaggtcgc
    13021 tatatttttg tgactgagtt tttagtaatt cttggtaacc ttttaatgca ctacctgttt
    13081 cagcatagac cgctttaaga cctttaatcc gcccgccatg cccttgaaag ctcttttcaa
    13141 ctgcttttaa agagttatct aagcctttca tgtaggtttt taggtttctt gtgtttgaca
    13201 taaagggtga tatgtctaat gtagctgttg ctactaaatc acctatattc cccatttagt
    13261 ctcctttcta gccgaaaagg aatgggaatg ccttatccaa ggtcgtttca atcacttctt
    13321 ctttttcgac agtttcaatt tctagtgctt gtaccatcaa ttcgatgtct gataagcgca
    13381 tttttttaat atctaaaatg gtataaccgt ttttcaaaag gctctgtatc cacaagagta
    13441 agttttcttt agcctcttgt ggggttattg ttcctttttt tcgctttcgt cttcctcctg
    13501 ctctcctccg agtgctgcaa tgtacaggtc attaagtgta tttaaaacgg tcatatcaga
    13561 ttgctttaaa tcatcaacgg taaactgacc tgcgtacata tcaacaaaca tttgcaagta
    13621 agattcgtta agttttctat gcgctttaga atctaaacgt cttttctcat cgctaaacac
    13681 agcgctttgt ctaacttgat gctcaacagc caacaaatta tcttcgacat tgataaagtc
    13741 ttttttaaat tccttatcaa caccgccttt ttttaaggta atttcgtaca tatctactcc
    13801 ttgtcaaaaa taaaaggtcg cagcttaaat gcgaccttaa aaattacata accgtacttg
    13861 cttgttccgc aattggtatt tttttaattt ctgcatcacc gcagccggca aatacacatt
    13921 ttaaaaattc attcaaatta aaaccgctat taccctcgcg cccgaccaaa agcacttcct
    13981 cttttggtcc gcgggcaaca aagttacccg ttgtagtatc tggtttcgga tcaggtgcgc
    14041 cttcttttgt ttgtgcctcc attcccggaa gattaaattt ccctttaagc aacgcaatcc
    14101 aaatcgcctt accgttttca tcgctagtcc taaataaaca aacaatgtca tttggcgtta
    14161 ggtttttgtt gtacttctca acaccttttt ctactgtgat tccaaagaaa tctttgcgtg
    14221 catctgatgt taaatctaac aactcgattt caagttttgt ctcgctgatt ccacctgata
    14281 atacaacgta aggtccatcg tctgctggca cagtgaccaa ttcattggta atatcaagtt
    14341 ttgcggattt cattcccggc aaatgcttta tactctctgc ttttgtaacc ttgtcatttc
    14401 cgtctagtac tccatagtaa aaatcacgta gtccaaattt tacttttccc attttttcct
    14461 ctttctttta ataaaaatca aaatagcggt atttccttac attcatgagt aagtcaatat
    14521 ctctatcttt atatcttggt ttttcattcg cggtatatct ttcaaaaccg ccttgtttta
    14581 aaatgttatc tatacatttg ttaatttggt cagcttctga agctgttttg caccaaaaat
    14641 taatagttat acgttgctca ttgcaaagta tctcgtcatc agcataatca aaaggaccat
    14701 ctaacgttgt gttaattcgc ataaatggtg ccaattctac ttttcgtaag ttaacaggat
    14761 tatccgggat atcataagta aaaatccctt gtttaaatcc atttttaaaa gtaccgcctc
    14821 tgagcctatc caacagctca tttagcgtct tatcgttact taacaattta taagctgctg
    14881 tttcagcaat caaagtccta gtccctcctt aactttttca gcataaattt gtctggctct
    14941 cggtgtcatc tgattgattg ttctttcctt gaaatcctgt ccacgttgat aaattgtccc
    15001 atcgttagga tagtgagcac gccaacctgt cgctttacca taaccgattt cttttgaaac
    15061 aatgccgaca ttagaacctt tgaaaccgct gataactgta tcttcttgta gccgctcatc
    15121 tgtttcaatc tcataaacag gtgtatttga tttgagggtc ttctcaaatt ctttcgcaac
    15181 cttagtcacg gcagcttttg cagtttttga agcttttact tcaagctttg tcagattagc
    15241 caatatttca tctaaaccct ttgtcatgac atgcttactc cacttatcat gatcatatct
    15301 ttgccggatt cgtcagattc gattttatct atcttataaa tgcgcttgtc aaactcaaca
    15361 aacatcgtgt tatctatgga taattcaggg ttatacctaa ttaaaaaaac ttttgtatcc
    15421 ttatttgtcg gcagattgct agcgttttga aactttgatt ggtaattaaa gtctcttaac
    15481 tgcgttttca atacttctgc ccaacaggta tataagtctt ttctgatagc agatactact
    15541 tcaccatctt cattttgccc acctgattgg ctaaaaatcg ttatcctgac gttcatcttg
    15601 cgtgtaatca tgaatcatca ccccgcaatc gtagttgatg gatgatattt aaaacgccat
    15661 ttgccaacgg atagcgaaaa ctatccgcag acaaaccgcg atggtcatac tcttccttga
    15721 cttgtttttt gactgctaaa gcaaatttag cgctttcctt aaacgtgcct gcggttgagc
    15781 cctcttctat cgcaaaacaa atttgttcct gagcagattc aatcatttct ttaatgatgt
    15841 catcctcaaa atcaaagtca attttacaat agagctttac actgtctaat aactctttcg
    15901 atacagccat ggctatacct cttcaacgcc tgctagtgca agtaaatctg atttcaatgt
    15961 cttaccacta aagtcaattc ccttgcttgt taaatagcgt ttgatttctt ctacagtgct
    16021 tttgcttgtt ggtttcgcca cttcttcagt ggccttacga gggcaattgt tctggcgtaa
    16081 atgtcacata gtagccagct ttgtcatcta ccttagcaac accgaaacgt aacacagctt
    16141 gcaaatattg accgtaaatg tcattatcga cccaacgcaa acctaagtct ttgcggtcgg
    16201 cgaataacac accacgtttg aaatctccga caaatgcttt atttttacct aaaacctcat
    16261 cggctagtac aaaaacaggt tttccaagga atactttccc agtaacagaa gtgattgagt
    16321 cttggagtag ataacggccg ttttcgtctt ttaaagtatc catggactgg taaaagcttt
    16381 gagatacgat aaatgacaca ttgtaagctg gatctaaatc tacatttagg atttttttga
    16441 tttcatctaa tgttttgaca tcagctttag gctcaaatgt ttttaggact ttagcaattg
    16501 catcattagt tgtattaact ttcatttgac cgattgtttc agcaacaatt ccaactaaat
    16561 caacatctgc atcgtcaatt gactcttgag ataatgggat ggctccacga taagtctcga
    16621 ctttccactc tacgtcttta aattttggtt tagcaagctt tggatttttt tcgagctctt
    16681 caacgctaac cattttttca gtcgcacatt gcaatactgg ccatttccct gacgcttttt
    16741 tagctgggtg gatgctagtg aattgtttca aatcaacaac tgtcttaact tcacgagctg
    16801 gtgtgtaaag aatttcttca ctagagacag gttttacatc tgttttctta acaccgtccg
    16861 ttttaggatc tataggagtc gtttggttaa gtgggataag aacctcgtct ttcccttcga
    16921 aacgcaaagc ttcgttagtt actgttcctt ttgaacgaat aaattcgtta acgctttcgc
    16981 ggtatgtttt agtttcttgt ggcacttctt ttcctccagt attttctgca ccgccttttt
    17041 caatgctagc ttcatataac ttcaagtcgt tttctgcttc tactaggtcc gcttttgctt
    17101 gttcaacttc tgctttaatt gagcgagcag tttcaaggtc atctgattcc aaagcatttt
    17161 ttacttcggc tgttttagta gcaatcgctt ggtttaaact agcgatagtc gctttaattt
    17221 ctttgatttt ttcttcgaac ataaattcct ccaataaaaa aagagcttat agcccttgta
    17281 aaatttcttc tttttcaatt tctagcaaca tgttgctaat ctcttgcttg cgcttacttc
    17341 tgtttgcgta atagtcgtca ataacagctt gtggcaacat ttggttatcg atactcgcca
    17401 ccgcttcaaa agacatcact tcatcagcaa aacctttttc aacagcgtct tgagcagaca
    17461 taaacgtttc attccgcatt aaattcatga tttcatcttc cgataaccct gtctttgcga
    17521 cataggcatt cacgatagct ttatcacttg atttcaaagc attagaagcc ttatctaagt
    17581 catcgctatt accagctacc caattaaaca atgccttgtg taccatcatc tgtgctgttg
    17641 gactcatgac aactttatct gctcccataa ctgcaacgga tgctgcactt gcagccatgc
    17701 cagtaatttc tgcagttact tttcctttgt aatttcgcaa agcggtatag acttcactcc
    17761 ctactgtaac aagaccaccg tttgaattaa cttccaaaat aatatcactg ctatcttctg
    17821 gcaattggtt aataatgctt ttggcactag tcgcttccat tccatagtag tcataaactt
    17881 cttgcgaatt atttgcaata agtgggcctt taagatttat cctctttggc atttacctca
    17941 cctcctttcc ctttgatacc aaccttgtcc tgatattctt cttttttatc caaaaagaca
    18001 tagtttagac tcgattggta cctatccata ttaggatctg tagatttctg cttacctaac
    18061 tcaataaggc cttggttagg tgtcagtatt tgattgttaa ccaatttgac aatctcatca
    18121 acattacggc ctgtaacgct acgtgtatca aattcaatac gatagagacg tctatcttta
    18181 tcatttaacg ttttaagacc taattcgctt gtaatcgcgt caaaataaaa aggcaagtcg
    18241 tttgtgacat agtcctccat cagttgagct acggattgat tagggctatt aacaccaagc
    18301 ttgtagctgg gaacccgcaa agcttttgcg atttgcgccg ttgaaaaatt gttactagtg
    18361 attaactgca atacgttagt atcaatttca agcggcgtgt attccatggt actatcaaac
    18421 accaatggac tgccaccgac cgaaccctcg cgcattttct caaactcttg acgtgctcgc
    18481 tgccgtgcgt ctccgcttaa ttgagcacct ttcatagtta agataccgct agaaaatcca
    18541 tctttaaaga atttaattaa ggtattgata ccacctgttt gcaaatcaat ctcatctcct
    18601 aaagacaata gcggagacct gccaaggatt gtgtcgtggc taaaaaactt ccagtgtaca
    18661 acatcatgag caaagcattt aacctgtttt gctgttaacg tatcagtaaa agtgtagatg
    18721 atttcgtgac tatccgtttc ctctactgtt gtttctgatg gcctgtaaaa ttggaattgc
    18781 aaagcttgat tagtctttgg atctctcaaa atacgcgaaa aagaattacc agtcaaaatg
    18841 gcatttactg ccatagcaaa tttccatgtc ctggcgctcg cattttttgt agatttaaca
    18901 tttaaaagat aattaatatc ctcatcatgg ataatgtccc cattaacatc ctttttaaca
    18961 agcgggaacc tagcaatatc cccagctata atagacgttg ctgttaaaat atcgctattc
    19021 ttcaaagccg acacccctaa gtatttttga gagacatcac cagctaaaac agatgatata
    19081 tagtcatcgt aggacacctt tgaactgccc aaaggtcgga aaaaactcat cgatttctca
    19141 cctcctttct aaaccattaa catctatttc cgccacttgc gcttataatt ttgcttgatg
    19201 tgaccaacct catcaccaat tgacttgata gctacttgat tgtctagtat cgctgattta
    19261 actgctgcaa gctcgttatt tgtagctttg gcatttgctg acacgatgga tcgtaattcg
    19321 gcgacttctt taattagcaa cacaatagct gtttccaatt ttcgtttttt cttaatgcgt
    19381 ttattcacgt tttcctccta cgctatctac atagatagct aaaacaatta aaataagacc
    19441 actggcaata aaaccgactt tatcaccaaa caaaaacaac ccatacatca ataaacctag
    19501 tccagctaac aagattaatg tgtgtatgtt ttttaaaata aaatcaatca aaacagcgat
    19561 tctcctccta ttattttttc atttgtccag tagccagaac catcgaacgg ttctaagtaa
    19621 caaacagcgt agccatcaag agcagcatca agcgggtcaa tcttattgct attcttattc
    19681 ttatcgatcc tcataccatt attatcagtt ttaatataag cattgttaat agccatggtt
    19741 agcaaagggt taccagagtg ctttattttg ccttttttga ggtcgtcacg aaattgtttt
    19801 gtcggcatgt tcaaaaccat tgtcgtttga ctaacctcaa tcagaggcca ctctggatgc
    19861 cttttttcaa tcattgtaag taatgttcca aattgataag ggtcaaaaca aattccatta
    19921 atttcccagt cgtttgagta gaccatctct tcaatttttt cgagtacacg ttcatcatcg
    19981 atgacaccac tttctagggt ggttatctcg caataacctt ggcattcaag gttgctataa
    20041 gatacgccat cccgcttttc tttagcagtt aaaccatatt tggtagccac aaaagaaaag
    20101 ctgtcgatat accaaaaatc atccatcata acaactggtg tgatagcaaa taagtcgcta
    20161 acacgaccaa cgtcaacacc taaccaaact ctccgcttat tagtatctgg cttatcaatc
    20221 cgagcaagtt cccaagtcgt tttatcgatg taagattcct cactagactg acgccacatg
    20281 ttaaagtttt tgattaatac tttattgatt tcaccagttt caagggacac tctacgacgc
    20341 gttctcaggt aatccattag cttatcatgt aaagcttcaa cctcaaggat tggatttgat
    20401 tttatccagt ttgattcgtc tttaatttcc tcttcgttgt cctgttctgc aacatatcca
    20461 aaataaccct cgtctgtgat ttctccgtct agtattttag tgatgtatgg atactcaatt
    20521 gtgtgcatag ggacattcaa atccatccct gccgtcgaaa taatcaagat aaacgggtta
    20581 tcaagctgac cttgaccaga ttctaaaagt tctaacattt cattcgtctt cgatgctgca
    20641 aattcatcca aaacgccaac ataaggctca aatccatcta cagccccagt atctcgacta
    20701 agtgctctga tatatgattc gtcatgtaag tttttaagct catcacggac aatttttgta
    20761 gcctttctga catcagcgtc cttcgctctg agagatgata actgcttctt tgccatatcc
    20821 caagcgattt tagcctgcgt tctatcattt gcagtacaaa acaattgtct gctcatagat
    20881 gggttatggc caaacaaaaa ttcgtaaagc aagataccag ctatcagaat tgttttaccg
    20941 tttttacgag caacagaaat catagctttt ctaaaacgtc tcaaggaatg atctgttttc
    21001 tttcgccatc catataaatt cccaataata aacttctgga acatcgccaa cgggtaaggt
    21061 ttccccgttt ttacatctgg caaaatctca ataaagttaa tcggatcagc tgctttttct
    21121 ggtaaataga tgaatttaaa attatcacca tctatttttt tgaggtcatt caaatgtctt
    21181 aaacaagctc taaatacctt cttacttgat tttatctgtt cgtcaacgac catttttgca
    21241 taataaaaag cgtcatcttt atagatgtcg ctgattgctg aataatcgta ttccactttt
    21301 atcacctaca aacctatacg gaatgattgg cctatcgttt gcatataata ccgaccacct
    21361 tcttcgctaa tatactccac taaaaaacca ttatccaacg gattgttttg ggcgtcatct
    21421 gaatcatact taacaattaa tttagtgata ccatttattt tctcaccgtt gatttcaaca
    21481 attggaaact taccattttc taatagctta acagaaaatt ctttaactcg ttccattttt
    21541 atcctccaaa tttatcaaaa atactcttag gcttttcctc ttctttagga ataaacattt
    21601 tcatccgact atccacagtc aacccaagct gacttgcgct acttctaata ttcgctgtgg
    21661 ctttttctag tgttaaaata agcggactag gaataatgcc cttgtctgga tcattcgtaa
    21721 aataaccaac ctcatctagt tttctacttg tttctttgta gatagcatac catgtgcaat
    21781 ataactctaa tagccctcta tcgagatttc ttaggggtag gttttggagg tcttctatga
    21841 ttctcctata ctcgtactta gctacattat taaagtgttc aggaggtgtc ttttgcaact
    21901 ttttaagacc gtcagacgcc ttattttgcg cggtttctcg cactattttt tcttctttcg
    21961 taagatgctt ttttgtcgtt tcgactagct ttaaatttct tcccataaga cctcctttac
    22021 acgaacttta cagttttaaa aatttcaaaa agggaatttt ttgcacggaa aagggcgcgt
    22081 tcttaagttt ccgaacaata tagccccgtt taaaaatgaa gggggtagtt tccgaatatt
    22141 aacatactgt tgttaatttt tacacccgtt tttacattaa ttgtttttac gtctttctcg
    22201 tatcgcttta ctatcattac atgctttaca gcttgcttgc aagttattcc aatctaatct
    22261 tttgttccaa tcttgtttga ctgatacaat gtggtcagtc atagtcgctt cgccaccaca
    22321 catagcacag atgtaatcgt tctgaagtaa tacttgctta cttgtctctc tccatatcct
    22381 tgagttataa aactgtttgg cttgtttatc gtacttccag cgattacgat tatagtcacg
    22441 atactctgct gatctatcat cgtagtcaac tgttgtacgt ctaccacctg cgatggttaa
    22501 cttctgtggc ctcataccct gacctcacca gttagcctat taatataatg aactaatgtt
    22561 gtgtatggaa tagtgtagcc gttatcaacg tatatattac ttacttcttt ttcccattgt
    22621 ggtttactat acggatactt atttggtctc atttgttatc tcctaatttt tgcgtatttt
    22681 atgcgtattt tgcattgaca tctacattgt ttatgtgtat aatataagta tagaaagtga
    22741 ggtaagcaat atgccaatga ccccaaagca gatgataaaa ctgctaaaaa agaatgggtt
    22801 ctacgaagtc agtcaaaacg gaagtcacaa aaaattccga gacaataacg gacatcaaac
    22861 cattgttcca atgcacaata aagacctcgg aaaaggtctt gaagatacca ttctaaagca
    22921 ggcgggttta aaataatccg cttgccagat ggtgtgctta tctcacagta atcaaaggag
    22981 gatcattatg ttaatttatc cagctacatt tacacaagac acagactata tcatggttac
    23041 atttccagat gtacctgaag caattactca aggtgaagac tttcaagagg cttatgaaat
    23101 ggctgtcgaa gtcttaggtt ttgcccttga agactatact gactatccaa aggctagcgc
    23161 cgtttctgat ttaaaagaac agtatcctga ttccgacatt gctttaattg gcatcgatat
    23221 ggttgcctat atgaaaaaat atcactccaa aaaagtccgc aaaaacgtga ctatccctga
    23281 atggttaaac gacgcagcag aagataaaaa cctcaacttt tctcaagttc ttactgaagc
    23341 gcttgaatta aaattacaag cataagagcc actgttgtgg ttctttttta cataataaaa
    23401 aaccacgacg ttgacctatc gaaacattgt aatttcaacg tttgtaacta ataagtaacc
    23461 atgtcgcgga ccataacaaa aaaaatcacc actatgtgat gactgccata aacagttttt
    23521 cagttctctt acccgctccg ccaccttgca tggtgcttct tgcagtctta aactcatata
    23581 cgcattcaaa acgttcatct gatatatcgt aactagaaat taaaacaatg ttgtttttag
    23641 acatcccaaa cgcccaatca taaaatgctt gactgttaaa atcaccttta taactttttt
    23701 gcgtggtatt ttcgtatgga ggatcaagat aaaaaatagc gtcttcaata tctgaaaatg
    23761 cttcgtaact ataatttgtt ggttctaact gttctaaccg tcctaactgt tgtaaccgtc
    23821 ctaactgttg taactgttct aactgtgtta gttttttatt tttttcaagc tctccttcat
    23881 cgtaaggctt gtcagctttt ttatacgttt cggtttgttt gtacccgcta aacacatcgt
    23941 gcttattaat tatttcaacc gctaagtcat acttcgtatc agaccagtct gcgccgtata
    24001 aataactgct taattcattc ccaaaactat ttactaacaa ttttaaatta tcatcaactg
    24061 acttaggttc tttttgccta attttgttaa attcatcacg actgataatg agtgttttta
    24121 tccattctct atcttgacta atcacacgct gaaacatatc tgttatatcg ttgtctaagt
    24181 cgttataatg cacttctaaa ccatttagca cacactcagc agtaatcgct ccgcctccgc
    24241 cgaaaacgtc gtatatgggt ttatctgcat caaagttttg tttaataatt tcgactattt
    24301 tcttactaat tttcttttta ctaccttgat acggaagtcc aactggtttc ccttttcgta
    24361 ttttcttttc gtctaattta agcattttca ccccacaata aaaagccacc acaatgtgat
    24421 gactaattgg ttaaccatag ataaatagca aatgaattct aagcctattg cctaccccat
    24481 tctgggacac ttctatttat caaacaggaa cagtcggaat cgaaccgact tacaaccgtt
    24541 gtccctacta accacaagca aggttgcgac ccttgtttta cttgtggtta atcaaatgag
    24601 tcaatctgtt gtgaatacgt acacgtagac tatacagccg atctatgtat cttttttacc
    24661 aatcgtcatt ggtaggaata tgactatcaa ccaagtaaaa accagtatat cagtgcgtga
    24721 ctgcctttcg ggatactggg tcgcgcctct tctgggcgct gattgagacg gcaggaatcg
    24781 aacctgcata cccagttgtc gtctcaactc gctgtaccgc catcaggcta caaaataaca
    24841 agtttgattg tagttaaagt tggcgactaa ataaatagtc agttggtaaa atggttatct
    24901 cttcttgtta ttttgctatg ctactatatt aacactgtaa ttagtattat gtagtatcaa
    24961 tttgtatcta attagtattt tttagtatca attccagact ttcctttcca cgcctaacaa
    25021 acataaagta tttattacga tttcctatgt ttaagcgttc tctggctctt tcatagtctc
    25081 catcacagtc aagataagtc gtgagtaaaa cgtgactctc acaaatgccc atgctttgca
    25141 caatcaaatt agccatttct tgtcgacggt cttttagttg ctcaatctga tcgctataat
    25201 agccgaccat gtgtaacatt tttatgtttt tgtcttcctg tgattgttta accccgccag
    25261 ataccttcat atcagaccac tgaggagact taacgagcga ccgactcatt aggttagcgt
    25321 ctctttcaag ggtctctatg agatgtggga tagtctttaa ttcttccaaa aaattattag
    25381 cttttgttgt cggaatgttg cccatctaac tctccattca tgatataata ttgttgggta
    25441 tttaatcatg aaggcgttcg catggacgtc tttttttgtg gagaaaagtc ctctctttcc
    25501 tttgtttttt gacacaggcg cacgatgtcg tgttaaagca ttagcgacgc cttggataat
    25561 cacggatgac cgataacccg ctttagattt gttttggtgt aaggaggttc tcgtttctat
    25621 ttttttaatt tcggtccacc cccacagagc catcgcaggc tc
//
LOCUS       DAUGHY010000023        25260 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.023, whole genome shotgun sequence.
ACCESSION   DAUGHY010000023 DAUGHY010000000
VERSION     DAUGHY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25260
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.023"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(525..911)
                     /gene="rplQ"
                     /locus_tag="VH752_000951"
     CDS             complement(525..911)
                     /gene="rplQ"
                     /locus_tag="VH752_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEQ8289313.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(926..1864)
                     /locus_tag="VH752_000952"
     CDS             complement(926..1864)
                     /locus_tag="VH752_000952"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEQ8289314.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            complement(1910..2293)
                     /gene="rpsK"
                     /locus_tag="VH752_000953"
     CDS             complement(1910..2293)
                     /gene="rpsK"
                     /locus_tag="VH752_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEQ8289315.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2311..2676)
                     /gene="rpsM"
                     /locus_tag="VH752_000954"
     CDS             complement(2311..2676)
                     /gene="rpsM"
                     /locus_tag="VH752_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEQ8289316.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2694..2810)
                     /gene="rpmJ"
                     /locus_tag="VH752_000955"
     CDS             complement(2694..2810)
                     /gene="rpmJ"
                     /locus_tag="VH752_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEQ8289317.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2836..3054)
                     /gene="infA"
                     /locus_tag="VH752_000956"
     CDS             complement(2836..3054)
                     /gene="infA"
                     /locus_tag="VH752_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEQ8289318.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(3172..3810)
                     /locus_tag="VH752_000957"
     CDS             complement(3172..3810)
                     /locus_tag="VH752_000957"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEQ8289319.1"
                     /translation="MNLLIIGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            complement(3960..5264)
                     /gene="secY"
                     /locus_tag="VH752_000958"
     CDS             complement(3960..5264)
                     /gene="secY"
                     /locus_tag="VH752_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEQ8289320.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            complement(5281..5721)
                     /gene="rplO"
                     /locus_tag="VH752_000959"
     CDS             complement(5281..5721)
                     /gene="rplO"
                     /locus_tag="VH752_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEQ8289321.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            complement(5936..6118)
                     /gene="rpmD"
                     /locus_tag="VH752_000960"
     CDS             complement(5936..6118)
                     /gene="rpmD"
                     /locus_tag="VH752_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEQ8289322.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            complement(6133..6627)
                     /gene="rpsE"
                     /locus_tag="VH752_000961"
     CDS             complement(6133..6627)
                     /gene="rpsE"
                     /locus_tag="VH752_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEQ8289323.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6646..7002)
                     /gene="rplR"
                     /locus_tag="VH752_000962"
     CDS             complement(6646..7002)
                     /gene="rplR"
                     /locus_tag="VH752_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEQ8289324.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(7107..7643)
                     /gene="rplF"
                     /locus_tag="VH752_000963"
     CDS             complement(7107..7643)
                     /gene="rplF"
                     /locus_tag="VH752_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEQ8289325.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            complement(7846..8244)
                     /gene="rpsH"
                     /locus_tag="VH752_000964"
     CDS             complement(7846..8244)
                     /gene="rpsH"
                     /locus_tag="VH752_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEQ8289326.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(8395..8580)
                     /locus_tag="VH752_000965"
     CDS             complement(8395..8580)
                     /locus_tag="VH752_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEQ8289327.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            complement(8596..9138)
                     /gene="rplE"
                     /locus_tag="VH752_000966"
     CDS             complement(8596..9138)
                     /gene="rplE"
                     /locus_tag="VH752_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEQ8289328.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(9162..9467)
                     /gene="rplX"
                     /locus_tag="VH752_000967"
     CDS             complement(9162..9467)
                     /gene="rplX"
                     /locus_tag="VH752_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEQ8289329.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYSKKSGEVLD"
     gene            complement(9546..9914)
                     /gene="rplN"
                     /locus_tag="VH752_000968"
     CDS             complement(9546..9914)
                     /gene="rplN"
                     /locus_tag="VH752_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEQ8289330.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QAIPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9939..10199)
                     /gene="rpsQ"
                     /locus_tag="VH752_000969"
     CDS             complement(9939..10199)
                     /gene="rpsQ"
                     /locus_tag="VH752_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEQ8289331.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(10225..10431)
                     /locus_tag="VH752_000970"
     CDS             complement(10225..10431)
                     /locus_tag="VH752_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEQ8289332.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10441..10854)
                     /gene="rplP"
                     /locus_tag="VH752_000971"
     CDS             complement(10441..10854)
                     /gene="rplP"
                     /locus_tag="VH752_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEQ8289333.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10858..11511)
                     /gene="rpsC"
                     /locus_tag="VH752_000972"
     CDS             complement(10858..11511)
                     /gene="rpsC"
                     /locus_tag="VH752_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEQ8289334.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11524..11868)
                     /gene="rplV"
                     /locus_tag="VH752_000973"
     CDS             complement(11524..11868)
                     /gene="rplV"
                     /locus_tag="VH752_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEQ8289335.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11884..12162)
                     /gene="rpsS"
                     /locus_tag="VH752_000974"
     CDS             complement(11884..12162)
                     /gene="rpsS"
                     /locus_tag="VH752_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEQ8289336.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(12302..13135)
                     /gene="rplB"
                     /locus_tag="VH752_000975"
     CDS             complement(12302..13135)
                     /gene="rplB"
                     /locus_tag="VH752_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEQ8289337.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(13153..13449)
                     /locus_tag="VH752_000976"
     CDS             complement(13153..13449)
                     /locus_tag="VH752_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEQ8289338.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13449..14072)
                     /gene="rplD"
                     /locus_tag="VH752_000977"
     CDS             complement(13449..14072)
                     /gene="rplD"
                     /locus_tag="VH752_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEQ8289339.1"
                     /translation="MANVKLFDQTGKEVSSVELNDAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            complement(14096..14722)
                     /gene="rplC"
                     /locus_tag="VH752_000978"
     CDS             complement(14096..14722)
                     /gene="rplC"
                     /locus_tag="VH752_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEQ8289340.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVILIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14970..15278)
                     /gene="rpsJ"
                     /locus_tag="VH752_000979"
     CDS             complement(14970..15278)
                     /gene="rpsJ"
                     /locus_tag="VH752_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEQ8289341.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGAKVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(15477..16766)
                     /locus_tag="VH752_000980"
     CDS             complement(15477..16766)
                     /locus_tag="VH752_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEQ8289342.1"
                     /translation="MIYNRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSGV
                     SIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGLL
                     SVVGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKIPMQV
                     SLLINVLNAIFSALSIYVWGFGLLGVAWATVLSRLVGVFLLCQFIPIKQVAKRLMRPL
                     DKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAMA
                     TATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTDA
                     QRSAMVVLLFSLLGAPATAGTLVYTAVWQGLGKAKLPFYATTIGMWVIRIGLGYVIGV
                     VWQYGLIGVWMATVLDNTSRWFILSKQFKKYQEITFH"
     gene            complement(17154..18170)
                     /gene="adhP"
                     /locus_tag="VH752_000981"
     CDS             complement(17154..18170)
                     /gene="adhP"
                     /locus_tag="VH752_000981"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEQ8289343.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPNVGHGKALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLKVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADIVVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVVPIVEKVPVDTAPEVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(18422..21064)
                     /gene="adhE"
                     /locus_tag="VH752_000982"
                     /gene_synonym="adhC"
     CDS             complement(18422..21064)
                     /gene="adhE"
                     /locus_tag="VH752_000982"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEQ8289344.1"
                     /translation="MTEGHNTVETTSVSVTIDALVQKGLAALAEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYEETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKPATWIAEQAGFTVPEGTN
                     ILAAECKEVSENEPLTREKLSPVIAVLKSESREDGVEKARQMVEFNGLGHSAAIHTAD
                     AELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNAVGDNVSAVN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTELMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVLTVPGFIAADTGMDVLTHATEAYVSQMANDF
                     TDGLALQAIKIVFDNLEKSVKTADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPASTP
                     EEAVESYAKAVYDLGCRVGIQMNFKAQGIDENEWKEHSRELAYLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYAERPGRRK"
     gene            complement(21374..23149)
                     /locus_tag="VH752_000983"
     CDS             complement(21374..23149)
                     /locus_tag="VH752_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEQ8289345.1"
                     /translation="MRIKWFSFVRVTGLLLVLLYHFFKNVFPGGFIGVDIFFTFSGYL
                     ITALLIDEYTKKESIDIIGFLKRRFYRIVPPLVLMILLTIPFTFLIKKDFIANIGSQI
                     TAVLGFTTNIYEILTGSSYESQFIPHLFVHTWSLAIEVHFYLFWGVFVWLLARRKETQ
                     KQLRGLLFLISLGIFAISFLSMFIRSFMTSNFSLIYFSSLSHSFPFFLGAMFATITGI
                     NETTVRFQKNVRLWPRQYVLAAMVGAFTLLLVLTVTLDFNHITTYLFGFALASLFASI
                     MIYAARVLHEQTPDVQEPKAITYIADISYGIYLFHWPFYIIFSQLMSHILAVILTVFF
                     SILFATVSYYIVEPLVQGRKPNLLGLEIDCSPYYKWIVGGTLALALLTLGTCMIAPKV
                     GKFEKQLLVSSLQQAQSNMERTHTLAAGDANALSDVGIIGDSVALRSSAAFSKIMPQA
                     QLDAAVSRNFKEAFDLFNNQIKSKSLSKTVVLAVGVNSLDNYSQAVQSFIEALPNGHR
                     LVLVSPYNAKNASQVAEARDYGLKLSKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSE
                     DSQGAELYVSTIQTAVEKSAKKPAK"
     gene            complement(23146..23547)
                     /locus_tag="VH752_000984"
     CDS             complement(23146..23547)
                     /locus_tag="VH752_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289346.1"
                     /translation="MINMQDIVKKWSITRAKLEIVSVIVILVCAISVFSVRISNKTSL
                     TYDKGRMHYTGYVINHKMNGEGKLVYPNGDIYEGTFKNGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
     gene            complement(23571..24008)
                     /locus_tag="VH752_000985"
     CDS             complement(23571..24008)
                     /locus_tag="VH752_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEQ8289347.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     KPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREGGVPDPWYTNDFEETYQLVRKGCQDWLSRLMNKEY"
     gene            complement(24146..25144)
                     /gene="ruvB"
                     /locus_tag="VH752_000986"
     CDS             complement(24146..25144)
                     /gene="ruvB"
                     /locus_tag="VH752_000986"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEQ8289348.1"
                     /translation="MARILDNDVMGNEEFSDRTLRPQYLREYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITAQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
     gene            complement(25191..>25260)
                     /locus_tag="VH752_000987"
     CDS             complement(25191..>25260)
                     /locus_tag="VH752_000987"
                     /inference="COORDINATES: protein motif:HMM:NF038244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quorum-sensing system DWW-type pheromone"
                     /protein_id="HEQ8289349.1"
                     /translation="IFAALLSFKVVQELSAVDWWRL"
BASE COUNT         7467 a         5323 c         4448 g         8022 t
ORIGIN      
        1 aattttgttg gaaacggctt aaagtgacag attgtaaaag taaaaaagat attttcacct
       61 ccaaattaaa agaggatttt aaaatactta ttaataaaat attgaatagg ggataaaact
      121 aacaatatag tttgagcatc tgtttgcgat gcaagttttt ttcggacatt taaacgaggg
      181 gattacaccg tttatttgcc catgagaggt ctttaaacac ctgcataagg ttttcttatt
      241 ctgatgacct tattgtctct gagaaaactt tataaacgca tcagaagagg cttctgtgtt
      301 attgttttac tcagcttaaa agcgggagtg tcacctcata atacaacaaa acgcctgctt
      361 ataaaaagtt tctatagctt agataccttt cataaacaag cgtcacatat taacaatatg
      421 atgagtgttc ccgctaggaa aaatcctagc ggtagaccag agctagacta agaataatct
      481 ctcgattact ccatcatcat aacactcaac aaaattgata aaaattaaac taattcaata
      541 attgccattg gagcagcatc tccacggcgt ggttctgttt taagaatacg agtgtatcca
      601 ccgttacgtt ctgcataacg aggtgcaatt tctgagaaaa gtttttgaag agctgtcgtt
      661 gatgtgtatt tatcggtagc ttcatcataa ttttctgatg cgatttcgtt acgaacgtat
      721 gcagctgctt ggcgacgagc atgaagatca ccacgtttac caagagtaat cattttttca
      781 actgttttac ggatttcttt tgcacgtgct tctgtcgtta caattgattc gttgatcaaa
      841 agatctgtcg tcaaatcacg aagcattgct ttacgttgtg agctagtgcg tcctagttta
      901 cggtaagcca ttttgtcctc ctatattatt tatcgttttt tagtccgaga cctaagtcag
      961 caagtttaac cttaacttct tcaaggctct tacgtccaag attacggact ttcatcattt
     1021 caggctcaga tttttctgtt aagtcgaaga cagtgttaat gcctgcacgt tttaaacagt
     1081 tatatgagcg tacggataaa tcaagctcct cgatggtgcg gtcaagtacc ttttcatcgt
     1141 tcactttttc agtttctttc attacttcgg tagcttttgc aacctctgtt aaatcagtaa
     1201 acaggtttaa gtgttcgatc aaaactcgag cagatagacc taaagcatct tcaggaataa
     1261 ttgttccgtt tgtcatgatt tcaatcgtta atttatcaaa gccatcattg ctacctacac
     1321 gggcaggttc aacttgataa ttaacttttt taactggcgt atagattgaa tctactgcca
     1381 atgtacccac aggtgcatca tcttttttgt ttccttctgc tggcacataa ccacgttttt
     1441 tagcaacagt cattgttgca cgtagagaat gtccttcagc gattgtaaaa agataatgat
     1501 ccgggttaac aagttcgata tcactgtcag taagaatatc accagctgtt acctctgctg
     1561 gtccttctac ttcaagttcg ataatctttt cgtcttcgac gtatgatttc acagcaagtc
     1621 ccttaacatt aaggataatt tgcatgacat cttcacgtac acctgggatt gtatcaaatt
     1681 cgtgtagtac tccatcaatt ttaattgatg ttactgctgc acctggaagt gaagacaaga
     1741 gtacacgacg aagagaatta cctagagttg ttccgtagcc acgttcaagt ggttcgataa
     1801 caaatctacc gtaatcttta ttttcatcaa tttttgttat tattggtttt tcaaactcaa
     1861 tcattttttc acccctcgaa acgaaacttg tgtactattt gttatatgat tatacacgac
     1921 gacgttttgg aggacgagca ccattatgtg gcacaggggt cacatcacga attgcagtta
     1981 cttcaagacc tgcagctgct agagcacgaa tagctgattc acgacctgaa ccaggacctt
     2041 ttacagtcac ttcaacagtt tttagtccgt gttcttgtgc agattttgca gcagcttctg
     2101 cagccatttg agcagcaaat ggagtagatt tacgagaacc tttgaaacca agagcaccag
     2161 ctgatgacca tgcaagagca ttaccatgca catctgtaat cataacaata gtgttattaa
     2221 atgtagcgtg aatgtgtgca acaccagatt cgatgttctt tttcacacga cgtttacgtg
     2281 ttggtttagc caattgtttt acctcctatt ttattttttc ttacctgcaa tcgcaacagc
     2341 tttcccttta cgagtgcgag cattgttttt agtattttgt ccacggacag gaagtccacg
     2401 acgatgacgg attccacggt atgaaccgat ttccatcaaa cgtttaatgt tcatgtttac
     2461 ttcacgtcga aggtcacctt caactttgat tgcatccact tcacgacgga tagcatcttc
     2521 ttgatctgat gttaaatctt taacacggat atcttcagaa atacctgcag ctgctaagat
     2581 ttttttagat gttgcaagac caattccgta aacataagta agtgaaatta ctacgcgttt
     2641 atcatttgga atatcaactc cagcaatacg agccattttt tctcctttct attttatcct
     2701 tgacgttgtt tgtgttttgg atttgtcgga caaattacca taacacgacc attacggcga
     2761 ataactttac agtattcgca aattggttta accgatggtc ttaccttcat gttttaatcc
     2821 ctccaattat ttcgattatt taaagcggta tgtgatgcgt ccacgtgtca agtcataagg
     2881 actcatttct actgtaacac ggtcacctac taaaatacga atgtaatttt tgcggatttt
     2941 tcctgataca gttgctagaa tttgatgtcc attttccaat tcaacagtaa acattgcatt
     3001 tggcatcgtt tctactactt tgccttcaat ttcaatcacg tcttcttttg ccacgcaaaa
     3061 gtacccccta aatttcgatt tgatgtcctc tgagcacaga ggtaacaatt ataagtcaga
     3121 ctaaactagt atatcattac taatctactt atgcaagtag attagtataa tttatttgag
     3181 ttctagcaac gctttttcaa catctgcaaa aacgtctgtt atttcttgat taccttcaat
     3241 atctgtaaca agaccaagct tacgatagtg ttcaagaata ggttctcctt gagccatatt
     3301 gacgtccaaa cgacgtttaa cagtttcagg cttatcatct tcacgttgat agtaatcttc
     3361 ttctttataa tctactggtg ggttgaacac tttatggaac gtttcaccag ttttacgatt
     3421 gataatacga ccactcaaac gctctacaag acatgatgga tccactttaa tattaataac
     3481 accatctaag cggagtccta gttcttcaag tgttgcatct aaggcgtgtg cttgttcaat
     3541 agtacgtgga tacccatcaa gcaaaaaacc tttttctgcg atatcatcct cagctaagcg
     3601 ctcttttaca atcccgtttg tgacttcatc aggaaccaat tcacctttat caatataact
     3661 tttagctaaa cgtcccattt cggtttgatt agccattgcg gcgcggaaca tatcccctgt
     3721 tgagatgtga gcaacaccaa attcttcaac aatcttagct gcttgggtcc ctttaccagc
     3781 tcccggcaaa cctatgatta aaagattcat cttttcctcc tcttttaaaa taaatacata
     3841 gtaggaaatt attttccttg gtttttgttt tcaaatggca acaagaaatc gtttgatgat
     3901 aaccaagtct tattgtcatt taaaaacaaa atagaaggtt gacaatgtca acctgtattc
     3961 tattctgctg tattcataaa tccgacatat tttctcttta gaagatatcc ctcaagctgt
     4021 ttcatacctt cgataccagt tgaaatcaaa atgagcaaac tagttccacc taaagcaata
     4081 ctagaagaaa ggttgagagc ttgttgtgct gctattggcg ctaaagagat aaatgctaag
     4141 aagatagctc ctacggttgc tagttttttt agcaatgcgg acataaattg ttctgtctca
     4201 cgtccagggc gaacacttgg tatatatgag gaattcttct gaagattttc tgctgtcttc
     4261 tcaggattaa cttgtacaaa ggtatagaag aatgagaaca atataatcaa caaggcgtaa
     4321 actatcattc cgactggagt ttgataatta aaaatttctt gcaaacggtt taaccacggt
     4381 aaatctctac cattttgaac aaaaggaata atcgtacttg ggatagttgt aatcgagcta
     4441 gcaaagataa cgggaataac gccggctgga tttactttta atggaagata tgaacttgta
     4501 ggtgcacctt gcattagctt tgtgtattgg attggaattt tatattccgc ttgttggaca
     4561 tatgttgtaa agaaaacaat agcaagaaca gctatgatta aaatcccaac aatgagataa
     4621 gacgagtgta aatcacttgc cttaacattt acaaaatagt cttcacgaat tgttgcaatt
     4681 gcacttggaa tagacgaaat aatacccgca aagataatca ttgatacgcc attaccaaat
     4741 cctttatctg tgatttgttc tccaagccag gtaacaataa cgctacctgt tgtcagtaat
     4801 gccccgatca gtaaatatgt tttgatgtct ggtgtcttaa caagtgcaac gttcgacaaa
     4861 gtattaaacc ctgcagtgat accaatggat tgggcaaaag ccaaaacaag tgaaatatag
     4921 cgcgtcgctt gatttaattt acgacgtcca acctcacctt gtttgcccca ctcaacaaac
     4981 ttaggtaaaa tatccatctg caataactga acaacgatag aagcagtaat atatgggcta
     5041 acccccattg aaaatactga gaaatttctc atcgcattac cactaactaa gtttaacata
     5101 tttaagaaag ggagttcact taattgctct aagctcttgg catttacacc aggtacggta
     5161 atatgtgtcc caattcggaa tacaagaatg ataaatatag taaagaaaat tttattccgt
     5221 acagtcttta tctttaacgc gtcttttagt atttttaaga acataatgag tatcccctca
     5281 ttagatgact tcgattgaac cacctttagc aataatagct gcttcagcag attttgagaa
     5341 cttagctgct tttacagtca attttttagt caattcaccg ttaccaagca ctttaacgcc
     5401 tgatttttca gctcggacaa taccagcatc ttttaaaata gccggagtca cttctgtacc
     5461 atcatcaaaa acattcagtt gatcaagatt cacaagtgca tattctttag tattaatatt
     5521 tgtaaagcca cgttttggaa tacgacggaa caatggtgtt tgtccacctt caaaacctaa
     5581 acgtacaccg ccaccgctac gagctttttg acctttttga ccgcgaccag atgttttacc
     5641 attacctgat gatgtcccac gaccaacacg gttacgtact ttacgtgagc cttcagcagc
     5701 ttttaattca tgaagtttca ttttttctcc tttttttgta aaatgctagc gcctctatga
     5761 gggaaaagga ctccctatag aaactcgcct atacattaat gatacttaag ttatccaaaa
     5821 taacttaata cagatacgca ctaaccgact actgtttgtt gccaaaaaag caaactacaa
     5881 gagtcggttt tgtgctcgtt tttaattatg aaatcagaga tttcacagtt ataacttatt
     5941 taacatcttc aacggtaacc aagtgagaaa tagctgtcac cataccacgg atagcagcat
     6001 tatcttcttt gactactgaa gagtttaatt taccaagtcc aagagcaaca acagttttac
     6061 gttgttctgg cttacgtccg ataggagact tagtcaaagt aattttaatt tgagccatga
     6121 tgatcccctt tcttatgcta agtcagaaac tgagatgcca cgaagtgcgg caacttcttc
     6181 tgcacgttta agttgtttca atccttcaac agttgcacga acaatgttga ttggagtgtt
     6241 tgaaccaaga gattttgaag taatatcagc aacacctgct aattcgatga cggcacgaac
     6301 tgcaccacca gcagcaactc cagaaccttc tacagctggt ttcaataata ccttagctcc
     6361 accgaagtta gtgtaaactt cgtgagggat tgttgtacca accattggta cttcgatcat
     6421 gtttttctta gcagcttcaa ctgctttacg gatagcttct ggaacttctt gagctttacc
     6481 agtaccaaat ccaacacgac catttccatc accaacaact acaagagctg caaagcgaag
     6541 acgacgtcca cctttaacaa cttttgtaac gcggttaatc gcaacaacgc gttcttcaag
     6601 ttcaactgca ttatctttaa atgccattat tttgtgtcct ccctattaga atttcaatcc
     6661 gttttcacga gctgcatcag ccaaggcttt aacacgtccg tgatagagat atccaccgcg
     6721 gtcaaacacc acttcagaaa tacctttagc cactgcacgt tcagcaacaa gtttgccgac
     6781 tacaacggct tgttctgttt ttgttccttt agaaacgtct ttatcaagag ttgatgcact
     6841 tgcgagcgtt acacccgcta cgtcatcaat aacttgagcg tagatgcctg tattagaacg
     6901 gaatacgttc aaacgtgggc gatcagcagt tccagagagt ttaccgcgga cgcgacggtg
     6961 gcgtttttgg cggattttgt ttttatctgg tttcgaaatc acaattttca cctcttaatt
     7021 ttaatcaatt tgtctcattg acaagttgtc gattgaactt atcgagcatg ttaaggtgca
     7081 atacttccca tacccgacta agaacattat ttacctgttt taccttcttt aaggcgtacg
     7141 tattcaccaa cgtaacggat ccctttgcct ttgtaaggct ctggtgaacg caagctacgg
     7201 atgtaagcag ctgtttgacc aacaacttct ttgttgattc cttcaactga gattgaagtt
     7261 gggttagcaa cagtgaaagt aattccttct ggagcttcaa cttcgtcttg gtgagattta
     7321 cctactgaaa ggacaagttt agtaccttga agttgagcac ggtaaccgac acccttcatt
     7381 tcaagatctt ttttgaaacc ttcagaaaca cctacaacca tgttattcaa gttagcacgg
     7441 gttgtcccat ggattgtttt catttctttt gagtcgttag gacgtacaac tgtgatttca
     7501 gtcccttcaa ctttgatttc aatatttttg ttaaactcac gagtgagttc gcctttaggg
     7561 cctttaacag taataacatt gttgttattt gttaattcaa cgcctgcagg catagtaatt
     7621 actttattac caatacgtga catgtttatt ttctcctgtt aaattgtcaa gccgcatagc
     7681 gactagtttt cacgggggtt tgatagaatg atatacttca atttatattt caaaattgaa
     7741 cacgagctgt ggtctgtgtg aaaaagataa atcttccttg agtgtaaaca cgctacgtca
     7801 gatttcctat tttcactttg tccacgacgc tcttagtatc ttaaattacc aaacgtaagc
     7861 gatcacttct ccaccaacat tcttttggcg agcttctttg tcagtcaaaa gaccttctga
     7921 tgttgaaatg attgcaattc caagtccgtt aagaacttta ggcatgtcat cacgttttgc
     7981 gtaaacgcga agacctggtt ttgaaatacg ttttaagtta gtgataacac gctcaccatt
     8041 ttttccgtat tttaagaaca cacgaatgat gccttgttta tcatcttcga taacttcaac
     8101 attttttaca aaaccttcac gtttaagaat ctcagcaatc ccttttttaa tgtttgaagc
     8161 aggtacttct aacacttcgt gttttacttg attagcatta cggatacgtg ttaaaaagtc
     8221 tgcaattggg tcagtcataa ccatttttta tattctcctc ttactagcag tttgatgcta
     8281 tcacttacta gttaatgttg tccttaaagg actttggtat cagcttatgc tcacaccgtt
     8341 ttaattaagc acaagactta atctgaagaa caaattaagt tttgtgtcat atttttacca
     8401 agaagctttg acaacacctg gaatttgacc tttgtaagcc aattcacgga agcaaacacg
     8461 acaaagttta aacttgcggt aaactgaatg tggacgtcca catttttcac agcgagtata
     8521 agcttgtgta gagtgttttg caggacgttt gttcttagca atcatagatt ttttagccaa
     8581 tttatttacc ccctattatt ttgcaaaagg cattccaagg cccttaagca attcacgtga
     8641 ttcttcgtca gtatttgcag tagtgacgat tacaatatca agaccacgta ctttatcaac
     8701 atcatcaaag ctgatttctg ggaagataag ttgttctttc acaccgagag tgtagtttcc
     8761 gcgtccatca aacgattttg ttggaacgcc atggaagtca cgtacacgag gaagtgaaac
     8821 gctaaccaat ttgtctagga attcgtacat acgttcgcca cggagggtaa cttttgcacc
     8881 gatcgctaca ccttcacgaa gacggaagcc agcgattgat ttcttagctt tagtaataag
     8941 tggtttttga cctgagataa gcgctaattc agctgcagct ttctctaggt tttttgcgtt
     9001 ggatacagca tcaccaacac ccatattgag aacaattttc tcaacttttg gcacagccat
     9061 aactgatgtg taattgaatt tctctgtcaa cgcaggaatt acttcgttag tatatttttc
     9121 ttttaaacga tttgccatta tgcttctcct ttccttcgtg attaatcaag cacttcgcct
     9181 gattttttgc tgtaacgtac ttttttaccg tcaacaactt tataaccaac acgtccagct
     9241 acaccatttt tatctaatac ttgaacatta gagacatgga taggtgcttc tttttcgacg
     9301 atagctcctt gaggattttc agtgttaggt ttttggtgtt ttttaatcat tccaacacct
     9361 tcaacaataa ctttgttaac ttttggaagt gctttaagaa ctacagcttc agtgcctttg
     9421 tccttgccag caattacgcg aactttgtcg ccttttttta caaacattct ggtttctcct
     9481 attatttctt acgccccgat gggcacccta ggaaaaccta ggggactacg tttgttaacc
     9541 tgagattaaa gtacttctgg tgcaagtgat acgatcttca tgtagccacc ctcacgcaat
     9601 tcgcgtgcaa ctgggccaaa gatacgagtt ccgcgaggag ttttatcatc acggatgatt
     9661 acagcagcat tgtcgtcaaa tttgatgtat gaaccgtctg gacggcgtgc accagttttt
     9721 gtacgaacga taacagcttt aaccacatca ccttttttaa ctgctccacc aggaatagct
     9781 tgttttacag aagcaacgat tacgtcaccg atgttagcga atttacgtcc tgaaccacca
     9841 agtactttga tagtcaagat ctcacgagca ccgctattat cagcaacttt caagcgagtt
     9901 tcttgttgaa tcatttcaat tttctccttt tagtttgatt agataataac agctttttcc
     9961 actacttcca caagacggaa acgttttgta gctgaaagtg ggcgagtttc catgatacga
    10021 acgatatcgc cttctttagc aacgttgttt tcgtcatgtg ctttatattt tttagaatag
    10081 ttgatacgtt taccatagac tgggtggtta cgtttagttt caactacaac tgtgattgtt
    10141 ttgtccatct tgtcagacac tacgcgtccg taaagagttt tacgttgatt acgttccatt
    10201 tagaatttct cctttcccaa tctattattt catttctgat tgcacagttt taacacgtgc
    10261 aatttgtttc ttaacttcgt caaggcgagc agtcttttca agttgacctg ctgcagcttg
    10321 gaaacgaaga tcaaacaatt cttttttgag ctcgttttct ttcttagcaa gttcttcttg
    10381 agacaatcca cgaagctctt taacaaaatc tttaatttct tgaagtttca tgtcttctcc
    10441 ttattctgct tcacgtttta cgaatttgca cttaactggt aatttgtggc tagcaagacg
    10501 taatgcttcg cgagcaattt cttcagaaac accagcaatt tcaaacatca ctttaccacg
    10561 tttaactggt gctacccaac cttcaggtgc ccctttacca gaacccatac gtacaccgat
    10621 agcttttgca gtgtatgatt tgtgtgggaa gattttaatc caaactttac caccacgttt
    10681 catgtaacga gtcatcgcaa tacgggcagc ttcgatttga cggtttgtaa tccagtggct
    10741 agttgtggct tgaagtccgt attcaccgaa tgaaacttct ttaccacctt tagcctcacc
    10801 gcgcattttt ccacggaatt cgcgacggtg tttaacacgt ttaggtacta acatttgtta
    10861 tttgcctcct ttagtgtttt tacgagctgg aagaacttct ccacggtaaa tccaaacttt
    10921 aacgccaagt ttaccataag ttgtgtcagc ttcttcccaa gcatagtcga tatcagcgcg
    10981 aagcgtgtga agtggaacag ttccttctga ataaccttca gcacgagcga tatcagcacc
    11041 gttcaaacga ccagaaactt gagttttaat ccctttagct cctgcacgca ttgtacgttg
    11101 gattgcttgt ttttgagcac gacggaaagc aacacgttgc tcaagttgac gagcaatgtt
    11161 ttcaccaaca aggtgagcat ctaaatcagg ttgtttgatt tcgatgatgt tgatgtgtac
    11221 ttgttttcca gttaatttgt taagttgacc acgaagagcg tcaacgtttg cgccaccttt
    11281 accgataacc atacctggtt tagcagtgtg aagtgaaaca ataactttgt ttactgcacg
    11341 ttcaatttca atagttgaaa ctgatgcgtc agctaattct ttattaatga atttacggat
    11401 tgcaagatct tcatgaaggt aatccgcgta ttctttttca gcataccatt tcgcatccca
    11461 gtcacggatg attccgacac gcataccaat tggatgtact ttttgaccca cgattttacc
    11521 tccttatttt tctgatacaa ctacagttac gtgagttgta cgtttgttga ttggtgaagc
    11581 tgaacctttc gcacgtggac ggaaacgttt cattgttggt ccttcgttag cgaatgtttc
    11641 agatactacc aagtttgctt tttccaaacc aaagttgttt tctgcgttag caattgctga
    11701 gttaagagtt ttctcaataa cacgagctgc tttgtttgga gtgaatttta agattgcgat
    11761 tgcgtcagca actttcttac cacggataag atcaagtact aaacgtgttt tacgaggtga
    11821 aacacgcact gtacgagcca ttgctttagc tgaagtaatt tctgccattg tgtcctcccc
    11881 ctattaacga cgtgttttct tgtcgtcagc tgcgtgacct ttgtaagtac gcgttggtgc
    11941 aaattcacca agtttgtgac ctaccatgtc ttcttggatg taaacaggta catgtttacg
    12001 tccgtcataa actgcgattg tatatccgat gaaacttggg aaaatcgttg aacgacgtga
    12061 ccaagttttg attacttttt tcttttcgtc gtttgcttga gcttcaactt ttttcatcaa
    12121 atgctcatcg acgaaaggtc cttttttaag actacgtccc attttgtagt tttctccttt
    12181 aaataatgta ccacagcggc ttgagttcaa gagcaagact ccaaaactct accgagttgg
    12241 cggaataaat agcaaagcta ttgtaacgct atttcagcaa gttttaagca acttactaag
    12301 attatttttc gttacgacga cgaacgataa gtttgtctga tttagctttc ttgttacgag
    12361 ttttaagacc aagcgctggt ttaccccatg gagttgatgg cgctttacgt ccaactggtg
    12421 ctttaccttc accaccaccg tgtgggtgat cgttagggtt cattacagaa ccacgaactg
    12481 ttgggcggat acctttccaa cggctacgtc ctgccttacc aatgttaaca agtgattgtt
    12541 gttcgttacc aacagtaccg atagttgcac ggcatgtacc aagaatcata cgaacttcgc
    12601 ctgattgaag acgaacaaga acgtatttac cttcttgacc aagtacttga gcagaagctc
    12661 cagctgcacg aacaagttct ccacctttac caggtttcaa ctcaatattg tgaacaactg
    12721 taccgacagg aatgtttgct aatggaagtg cgttaccaac tttgatatct gcatctggac
    12781 cagaaacaat acgttgacct acttcaagac ctttaggtgc aatgatgtaa gctttcacac
    12841 cgtcagtgta atgtacaagt gcgatgtttg cagtacggtt tggatcatat tcgattgttt
    12901 taacaactgc ttcaacgcca tctttgttac gtttgaagtc gatcaaacgg taatgacgtt
    12961 tgtgtccacc accttggtga cgaactgtga tgcgaccatt gttgttacga ccagctttgc
    13021 tcttaagaga aacaagcaat gatttctcag gcgtgcttgt tgtgatttcc gcgaaatcca
    13081 aagaagtcat gttacgacgg ccatttgtcg ttggtttata aactttaata cccacgttat
    13141 ttcctcctta gattattcag cttcagctgc gaacaactcg attgctttag aatcagctgt
    13201 aagagtgatg atagcttttt tagtttttga agtgaaacct gtgtaacgac caacgcgttt
    13261 tgcttttggt ttaacgttaa cagtgtttac acttgcaact ttaactccgt caaaagcagc
    13321 ttcaacagct tgtttgatca aaagtttgtg tgcacgagta tcaacttcga aagtgtattt
    13381 gcctgcttca agagcaatca ttgacttctc agtgataact ggttttttga ttacgtcgta
    13441 caaattcatt atgcaagaac ctcctcaatt gtagagattg cttctttagt aacaagaagt
    13501 ttgtctgcgt tcacgatatc aagaacactt gcagttgctg cagttgcaac agttacgttt
    13561 ggaaggttac gtgcagaaag tgctgcaaat tcatttcctt cttcaacaag aacaagtact
    13621 tttgtatcaa tgctaagagc tgaaagcact tttgcaaatt cagcagtttt tggtgctgca
    13681 aatgaaaggc cttctacagc tacaaattta tcttcagcaa cttttgctga gtaaactgat
    13741 ttcaacgcaa ggcgacgaac tttttgtgga agtttgtatc cgtatgaacg gggagttggt
    13801 ccaaagacaa caccaccacc acgccattgt ggtgagcgga tagaaccttg acgagcgcgt
    13861 ccagttcctt tttgacgcca tggtttacgt ccaccacctg atactgctga acggttttta
    13921 accgcatgag taccttggcg aaggctagcg cgttggctaa ttacaacatc aaaaacaact
    13981 gattcgtttg gttcgatacc gaagatagcg tcgtttaatt caactgagct aacttcttta
    14041 ccagtttggt caaatagttt tacgtttgcc attttaactg ttttctcctt tcttattatt
    14101 tagcagcttt aactgctgac ttgatagtga taagagattt cttagcacct ggtacgttac
    14161 ctttaataag gataacgttc ttttctggga taacttgtac aatttcaagg ttttgaactg
    14221 ttacacggtt accacccata cgtcctgcca agcgtttgtt tttgaaaacg cggttaggcg
    14281 caacaggtcc catagaacct gggcgacgat ggtaacgaga accgtgagcc ataggaccac
    14341 gtgattgacc atggcgtttg ataacacctt ggaaaccttt accttttgat gtccctgtga
    14401 cgtcaacaac atcaccagct tcaaattgtt ctacagataa ttctgcacca acttctaagc
    14461 cttcaatgtt tttgaattca cgaatgaagc gcttaggagc tgtgtttgct tttgcaacat
    14521 ggcctttggc aggtttgtta ctcaagactt cacgtttgtc atcaaaacca acctgaactg
    14581 cttcataacc gtctgtttca acagttttaa cttgaagcac aacgtttgga gttgcttcaa
    14641 tgacagtaac agggatgaat tcgcctgatt cagtgaagat ttgagtcatt cccacttttt
    14701 tccctaagat tccttttgtc atgagaaaat atttcctttt cttaataata tttcaaaaag
    14761 ttttttatga gcgttttttg tgctcaaaaa agttttttac gagcgttttt tgtgctcata
    14821 gcaagataca aagagcgtca aactcaaaat aaaaatagaa aatctaacac aaaaacttct
    14881 gtttcgagga agatttatct ttttcatgta gagtttagct cgtgttcaag ttccatcaaa
    14941 cactaactgt acttgcaaaa tctcaccgat taaagtttga tttctacgtt gacaccactt
    15001 ggaagatcaa gtttcatcaa agcatcaaca gttttttgtg ttggattgat gatgtctacc
    15061 aaacgtttgt gtgtacgcat ttcaaattgt tcgcgagaat ctttgtattt gtgagtcgca
    15121 cgaataattg tgtaaagact gcgttcagtt ggaagtggaa ctggtccagc aactttagca
    15181 cctgtacgtg ttgcagtttc aacgattttt tctgccgctg tatcaagtgt acggtgttcg
    15241 tacgctttca aacggatacg gatttttttg tttgccatct tttctcctct cgtctattta
    15301 cgataatagg ctagctcctc aagaaaaccg acacctgttg cgtggcaatg caaccgagcg
    15361 tgtcgcaacc tcttgtatca ttgctaaagc tgtattttta cagcaccaca atataataac
    15421 agaatcattt agtcattgca agctttttag ggcatttttt caaaaaaact accttcctaa
    15481 tgaaaggtaa tttcttggta ctttttaaat tgtttagaga gaataaacca ccgactggta
    15541 ttatctaaca ctgtcgccat ccagacccct atgagtccat attgccaaac cacaccaatc
    15601 acataaccaa ggccaatgcg aatcacccac attcctatgg ttgttgcata gaagggaagc
    15661 tttgcttttc ccaaaccctg ccagacggct gtataaacta aagtgcctgc cgttgcggga
    15721 gctcctagta aagaaaaaag taaaacaacc atggcggacc tttgagcatc agtattctga
    15781 gtaaatagcg gcaagagact aggacctagc agataggtca aggctcccat aaccagcatc
    15841 atgagggtgg ataaaataaa agcttctcta ataatatacc ttatctcagt caccttaccg
    15901 ccccccagtt gcctggccac tagaataatt gttgccgttg ccatggctaa gccaggcatg
    15961 taattaaact gagtcaaggt ttctccaata gcattacctg ccaaggccgt tgtcccaaaa
    16021 cgaacaacaa tgccaataat taggacatct cctgctctca tcatcaaacg ttcaccagca
    16081 gcaggtagtg ataaatcaaa aataattttg tccaaaggtc tcatcaatcg tttcgcaacc
    16141 tgtttaatag gaataaactg acaaagtaaa aacacaccca ctaaacgaga taaaaccgtc
    16201 gcccaagcaa caccaagaag accaaatccc catacataga tagacaaggc agaaaaaatg
    16261 gcattgagta cgtttattaa taagctcact tgcataggaa tcttaggata cccttgagct
    16321 cgaacaatag ctcctaagct agttaaaagt cctagactaa caatcatacc gccgacaatg
    16381 gatagatatt gccctccaac caatgttaca gaagcttcag ctcctagcca ttctaacacc
    16441 tgatgatgtc caacaactga taacaaccct aagccgaccg aaaggaggag tgttacttgc
    16501 aagacacctg ccatatagtt taattgtttg ttctgattat tttctccaat acttcgagca
    16561 atcagactag atacagcggc tcccagcgcg ataaagaggg actgataaat actgataatg
    16621 ttgttagcta tggagacacc agagactgct acaagtccaa tttgagccac caggtaatta
    16681 tcaaccattc ccattaacat ctgtaaaata ttttctatca tagaaggcag agctaaagag
    16741 aaaatttttc ttctattata tatcatttat gtcccctaat aaaaaattct tgatcaaacg
    16801 atttttttaa acacagtcac tggactcatt cagttatttt gttccattaa tgaattcata
    16861 aagtctacct taatagcaga gtcccaagat acacgacact tagtgttaca gtaacaattg
    16921 accacatgcc aatccttaac atttacctaa cgtttataac taagaagacg aagaacaaaa
    16981 ggactccaaa cgaatttttt ctattatata atatattctt catttagcaa ctttcaatgt
    17041 atcaaaatag cactctcgag tcaaaaatgt tttctgatac tcgagagtgc tattttattt
    17101 ggaaccaaat ttaagcaact taggcttatc tcatatttaa tacatttgta tgtttagctg
    17161 atgaaatcta agaccttgcg cccttggatt aaaccacgtt ccatttcatc aaagacttct
    17221 ggagcagtgt caactggaac tttttcgaca atcggaacaa ccagaccttc tgcaccaaag
    17281 gcaaaggctt cttccaagtc tttacgtgtc cctacaagtg atccgaccac tttgatacca
    17341 tctaaaacag tttttacgat agacagttcc atgtattctg atggaagacc tacggctaca
    17401 actgtcccac cggcacggac actatcaatt gcttggttaa aagcaacttt agaaacagcc
    17461 gttaccacaa caccatgtgc tccaccggtt ttttcttgaa tatagcctgg aacatcttca
    17521 atttctttgc cgttaactac aatatctgcg ccaacttctt tagctaactc aagtttatca
    17581 ttattaatgt ccactgcgac aacatgtgca ttaaagactt ttttagcgta ttgaacagcc
    17641 agatttccaa ggccacctgc cccaaagata acaatccatt ggcctggagc agcacctgcc
    17701 tctttaatag ctttataagt tgttacacca gcacaagtaa tggaagaagc ttgagccggg
    17761 tctaaacctt ctggaacttt aactgcataa tcagctgtta ctactgcata ttcactcatt
    17821 cctccatcaa cactgtatcc agcattttta acactacgac aaagtgtctc acgtcctgta
    17881 gtacagtatt cgcaatgacc acatccttca aagaaccatg caatagagac acgatcacca
    17941 acctttaagg atgttacgcc ttctccaatt tcttctacta taccaatacc ttcgtgcccc
    18001 aaaatacgtc ctggtacctg accaaaatcg ccatgtgcaa cgtgtaaatc agtatgacaa
    18061 acgccacagt attctacttt aacaagcgct ttaccatgac caacatttgg aagatcatgc
    18121 tcaacaactt cgacacctgt actagcttga ttaacaacta cggctttcat atcagtcctc
    18181 catctaataa tgactttttt gtaatcggtt acaaataaat tgtacatctt ttcacaatga
    18241 aagtcaatag aaacctgaaa agtttctgaa acttaactgg ttaatcttat agctgtttgc
    18301 ctatctgcaa aatacttaac tacattttct gacactggat ataaatgata tatttagaaa
    18361 ataaaatgat ttcttatgac tttaatcata agaaatcatt tcctggtatc aacattaaaa
    18421 atcacttacg tcgtcctgga cgttcagcat aaccatagta agcatcttca ataatttctt
    18481 gcatgtgatc aaccattgga agacgtgggt tagcaggtga acattggtct tcataagcaa
    18541 ggtaagccaa ttcacgagaa tgttctttcc attcgttttc gtcgatacct tgagctttga
    18601 agttcatttg aataccaact cggcaaccca gatcataaac agctttcgca taagattcca
    18661 ctgcttcttc tggtgtagaa gctggaagcc ctaacaattt cgcaatgtct tggtattttt
    18721 catccgcacg gtagtaatta tacttaggcc atgttgctgt tttagctgga cgagttccat
    18781 tatagcggat aacatacggc aaaagaattg catttgtacg tccgtgtacg gtatggaatt
    18841 gtgcgccaat tttatgcgcc attgagtgag aaatacctaa gaatgcattg gcgaaagcca
    18901 tacctgccat agttgacgca ttgtgcatct tttcacgtgc ttcaaaatcg gctgttttaa
    18961 ctgatttttc aagattatca aaaacaattt taatggcttg aagagctaaa ccatccgtaa
    19021 agtcattggc catttgtgac acgtaagctt cggttgcgtg tgtcaaaacg tccataccag
    19081 tatcagcagc aataaagcct ggaacagtca ataccaaagc tgggtcaaca atggctacag
    19141 ttggtgttaa tgagtaatca gcgattggat atttacgatt atttgctttg tcagagataa
    19201 cagcaaatgg tgtcacctct gaacctgttc cagaagttgt tgggatagca acaaattttg
    19261 ttttcttacc aagttctggg aatttgaacg cacgtttgcg gatatccatg aatttttgaa
    19321 cgaggtcatg gaaatcaact tctggttgct cgtagaagag ccacataact ttggctgcat
    19381 ccataggaga tcccccgcca agggcaataa tggtgtctgg tttgaaggta cgcattaatt
    19441 cagtaccttt cataacagtt gtaatgtctg gatctggttc tacttcagca aagatctgat
    19501 aaacaacttt attgcgacga agatcaagtt gttcaatgat acgatccaag aaaccaagct
    19561 caaccatagc gtgatctgta acaatcatga cacgttcaac gtcacgacat ttttgcaaat
    19621 attgaattga atcacgttcg aagtatgttt ttgaagggac tttaaaccat tgcatattat
    19681 ttctacgtct tcctactttt ttgatgttca agagattaac agcactaacg ttatcaccaa
    19741 ctgcgttacg tccatacgaa ccacaaccaa gagtcaatga tggtaagaaa gcattgtaaa
    19801 catcaccgat accaccaaat gtagaaggtg aattccagat gacacggata gcacgaattc
    19861 tagtaccaaa ttcttttgcc aactctgcat ctgctgtatg aatggcagct gagtgaccaa
    19921 gtccattaaa ttcaaccatt tgacgagctt tttcaacacc gtcttcacgt gattcagatt
    19981 tcaaaacagc aatgactgga gataattttt cacgtgttaa aggctcattt tctgaaactt
    20041 ctttacattc tgcagcaaga atattggttc cttctggaac agtaaatcca gcttgttctg
    20101 caatccaagt agctggtttt cctacaatat ttggatttaa ttttgcacca gcacagtttt
    20161 tgctatttgc tttggcacca aaacagaatt cttcaagaag ggctttttct tttttattta
    20221 caaaataagt atggtaagat ttgaattctg ctacaaaatc gtcatagatt tctttatcga
    20281 tgataacagc ttgttcagag gcacagacca taccattatc aaatgattta gacatcacaa
    20341 tatcatgagc ggcttgacgg atattagctg atttttcaac ataagctgga acgttaccag
    20401 cacctacccc aagggcaggt ttcccacatg agtatgccgc tttaaccatc gcatttccac
    20461 ctgtcgcaag gatagtggca atgccgtcat gattcatcaa agcatttgtt gcttcaagtg
    20521 atggtgtttc aacccactgc acacaatttt caggagcacc agcggcaata gcagcatcac
    20581 gaacaatacg agcggcatgt gctgatgatt cttgtgctga tgggtgaaag gcaaaaataa
    20641 ttggattacg tgtttttaac gagatcaaag atttaaagat agctgttgaa gttgggttag
    20701 ttgtgggtgt aatcccacag ataacaccaa ccggctcagc aattaatgtc aaacctgtca
    20761 catcatcttc ttcaataatt cctactgttt tttgatggcg catgttatta accacatgct
    20821 cacaagcaaa tagatttttt gttgctttat cttcaaagac accgcgacct gtttcttcat
    20881 aagcatgttt agcaagttca ccgtgagcat ccaaagcagc aacagaagct ttggctacga
    20941 tatagtcaac ttgttcttgg tcaagcttac gcatctctgc aagagcagct aaaccttttt
    21001 gaacaagagc atcaattgtc acagaaacag aggttgtttc aacagtatta tgtccttcag
    21061 tcatttgaga ctcctttatc ttgttaaaag tttcacaatt ttgtttacaa aattattata
    21121 gcactttttg aaattttgta aatcatttca caataaattt gtgaagtttt tttcaaatta
    21181 tttctgaaat cctatggtat caatattctt cattaacttt ttttcagaaa tatatctaaa
    21241 aaaagccgtt ttatttctga aaccgctttc ttttaaaaaa cctcaaagta ctactatttt
    21301 acaatcatca tttgttcaca agaaaaagcc agagcacaat ggatctgact tatctcttag
    21361 acatttctta actttattta gctggttttt tagctgattt ctcaacagca gtctggattg
    21421 tggagacata tagttctgct ccttgactat cttcactata atgaacaccg tcactgccat
    21481 accaaatatc tggatgttca acagcaactt tataccagtc tgcaatagtg acatacttgt
    21541 attttttgga caatttaaga ccatagtctc tagcttctgc aacctgactg gcatttttgg
    21601 cattatatgg agaaaccaaa actaaacgat gaccatttgg caatgcctca ataaaagact
    21661 gaactgcttg agaatagttg tcaagcgagt taacacctac tgctaagaca acagtcttag
    21721 atagactctt agatttaatt tggttattaa ataaatcgaa agcttcttta aaattccgac
    21781 tgaccgctgc atccagttgc gcttgtggca taatttttga gaatgctgca ctagagcgta
    21841 atgctacaga atcaccaata atacctacgt cactcaaagc attagcgtct cctgcagcta
    21901 gtgtatgtgt ccgctccata ttactttgag cctgttgtag ggaactaacc aacaattgtt
    21961 tctcaaactt accaacttta ggcgctatca tacaagttcc taaagttaat aatgctaagg
    22021 cgagagtccc tcctacaatc cacttatagt aaggagaaca gtcaatttct aaaccaagca
    22081 ggtttggttt tctcccttgg accaaaggtt ctacaatata gtaagacaca gtggcaaaca
    22141 agattgaaaa gaaaactgtt agaataactg ccaaaatatg agacattaat tggctaaaaa
    22201 taatgtaaaa tggccaatgg aagaggtaaa tcccatagct aatatcagct atataagtga
    22261 tagctttagg ctcttgtaca tctggtgttt gctcatgaag aacacgcgct gcataaatca
    22321 taattgatgc gaataaacta gccaaagcaa agccaaatag ataggtcgtg atatggttaa
    22381 aatccaatgt gaccgttagc acaagcaaaa gtgtaaatgc tccaaccata gcagcaagga
    22441 catactggcg cggccataaa cgtacatttt tttgaaatcg aacagtagtc tcgttaatac
    22501 ctgttatcgt ggcaaacatc gcccctaaaa agaagggaaa actgtgagaa agactcgaaa
    22561 aataaatcag ggaaaaattt gaggtcataa aagatcttat aaacatgctc aagaaactaa
    22621 tagcaaatat tcccaaagaa atcaaaaaca gtagtcctct cagttgtttc tgagtctcct
    22681 tgcgtctagc taataaccaa acaaatacac cccaaaacaa gtaaaaatga acttcaatag
    22741 ctaagctcca cgtatgaaca aagagatgtg gaataaattg gctttcataa ctacttcccg
    22801 tcaatatttc ataaatatta gtggtaaatc ctaaaacagc tgtaatctga cttcctatat
    22861 tagcaatgaa atccttctta attaaaaacg taaaaggaat ggtcaataaa atcattaaca
    22921 ccaaaggagg aacaattcga taaaagcgtc tttttaaaaa accgataata tcgatagact
    22981 ctttttttgt gtattcatca atcaagaggg ctgtgattag atatcctgaa aaggtgaaga
    23041 aaatgtcaac accaataaaa cctcctggaa atacattttt aaaaaagtgg tagagtaaaa
    23101 ccagtaaaag tcctgttacc ctaacaaacg aaaaccattt tattctcatt tttgaaatat
    23161 cccctgttta aaaatccctt tatagacttt attattcttt gcttttaaca cacctttgcc
    23221 atttgcttgt cctttatgaa attccccgtt atataaccag cccgtcttag cagtaaaagt
    23281 tccttttcct tcaaataggc cattcttaaa agtaccctca tagatatcac cgttggggta
    23341 gacaagcttc ccttctccat tcattttatg gttaatcacg taaccagtat agtgcatacg
    23401 ccctttatca taggtcaaac ttgtcttatt cgaaattctt acgctaaaaa cagaaattgc
    23461 acaaaccaat atgacaatca ccgagacaat ttccaacttc gcccgtgtaa tactccattt
    23521 ttttactata tcttgcatat ttatcatttc ttttactcaa cttctaatta tcaatactct
    23581 ttattcatta aacgagaaag ccaatcctgg catccttttc taactaattg gtaggtttct
    23641 tcaaaatcgt tagtgtacca aggatcagga acaccccctt ctctaaacag atagatttta
    23701 gagtcccatt gatgttgcga catctctttg agatttttca cattatcgct atccatacca
    23761 ataatatagt caaaggtgtt aaaatcagtt atagttatct gcttggagca tttagttata
    23821 tcataattaa tttggtaagt ttttaatatg gactgtgtac ctgagtgaat aggttttcca
    23881 tgctcccaat cagaagtagc acgactttct atcatcataa catcacttga tactattgac
    23941 ttcattacaa actcagccat ggggcttcga caaatattac ccagacagac aaaacatact
    24001 tttttcatta aatactcctt tgaattatta tatcaaattt ctaactgata tcggagaaat
    24061 caatctaata attttctgtc aacttatcaa aaaatccgaa cacaaaaagc aggttcagat
    24121 taattttctt agatttgaaa aatcattaag tgttttgata gggatagcca agatgacgat
    24181 aagccttttg agtcgctaca cgacctgttc gtgttcgcat gagaaagcct ttttgaatta
    24241 agtagggttc atacatctct tcaactgtat tacgctcctc ggcaatattc acagataatg
    24301 tccccaaacc aactggtcct ccctgataca tctcaatcat cgttcgtaaa attttctgat
    24361 caatgtaatc gagaccttcg cggtcaacat ctaacatagt caaagctcta tcagttattt
    24421 gagccgttat gatgccatca ccaataattt gagcatagtc acgcactcgt tttagtaatc
    24481 gattagcaat gcggggcgtt cctctactgc gacaagctaa tttccgtgca gcttcatggt
    24541 ctatttttat ttcaaaaata gtagctgtac gctccacaat ctctgtcaaa tccttttctt
    24601 gatagtattc catgtgacca gtgataccaa aacgagctct gaggggatta gataacatgc
    24661 ctgcacgtgt agtagcacca atcaatgtaa atggaggcag atccaaatga atactacgac
    24721 tagtatctcc agcgccaatc ataatatcaa tatagaagtc ttccatagca ctatatagta
    24781 cttcctcaac agacattggc atgcggtgaa tttcatcaat aaacaaaata tcccctggtt
    24841 ctaattcatt taaaatagct accaaatctc cagctttctc aacggcagga cctgaagttt
    24901 gctttaaatt aactcccaat tcattggcaa tcacaaatgc cattgttgtt tttcccagtc
    24961 ctggaggtcc aaataataaa acatggtcta aagattcgtc acgtctttta gctgcttcta
    25021 taaaaatagc gaattgttcc ttaactttat cttgtccaat atattcgcgt agatattgag
    25081 ggcgaagagt ccgatccgaa aactcctcat ttcccattac atcattgtct aaaattctag
    25141 ccataggact attataacat aaagtaaaag agagcagtgg cctactttta ttataatcgc
    25201 caccagtcaa cagcactaag ctcttgaact actttaaaag atagtagagc tgcgaatata
//
LOCUS       DAUGHY010000024        24900 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.024, whole genome shotgun sequence.
ACCESSION   DAUGHY010000024 DAUGHY010000000
VERSION     DAUGHY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24900
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.024"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..1143
                     /locus_tag="VH752_000988"
     CDS             <1..1143
                     /locus_tag="VH752_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEQ8289350.1"
                     /translation="SFVEKKLTAGVQLLDEKDFSNIKEKNQLVQLVTKSNRPIGTAYI
                     SKQNKGIGWYLGPEKIDLSISYFVSLFSVAKAKRQDFAQSDETNAYRLFNQEGDGFGG
                     VTIDLYKDFAVFSWYNAFVYDKKEMIMEAFQQVFPEVKGAYEKCRFKGPDTETAHLYG
                     ELAQETFSILENGIAYQVFLNEGLMTGIFLDQHDVRRALVDGLAMGKSLLNLFSYTAA
                     FSVAAAMGGAIETTSVDLAKRSRELSLAHFEHNQLNLASHHFVVMDVFEYFKYAKRKK
                     LIFDVIVIDPPSFARNKKQTFSVSRDYHKLITEALDILSPKGTIIASTNAANMTVSQF
                     KKQIIKGFGSRRPESMTLQQLPSDFTINKADERSNYLKVFTIKVRE"
     gene            1140..1826
                     /locus_tag="VH752_000989"
     CDS             1140..1826
                     /locus_tag="VH752_000989"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEQ8289351.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVTLNDMKRI
                     IEVLEMDISN"
     gene            1920..3086
                     /gene="aroC"
                     /locus_tag="VH752_000990"
     CDS             1920..3086
                     /gene="aroC"
                     /locus_tag="VH752_000990"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEQ8289352.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGVGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            3147..3488
                     /locus_tag="VH752_000991"
     CDS             3147..3488
                     /locus_tag="VH752_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEQ8289353.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADASASELFDE
                     FVAMQEKIQGMMQSGQMPTAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            3709..5061
                     /gene="gorA"
                     /locus_tag="VH752_000992"
     CDS             3709..5061
                     /gene="gorA"
                     /locus_tag="VH752_000992"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEQ8289354.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHGAKVLLAEGKKIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKEKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKAIVDVLVEEM
                     AVGGPCLHPHSEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFGLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSERLFNGKIDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKQYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(5149..6417)
                     /locus_tag="VH752_000993"
     CDS             complement(5149..6417)
                     /locus_tag="VH752_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ8289355.1"
                     /translation="MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTFSFQHDNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISEVRQYCLSNANIFKNIFP"
     gene            complement(6447..6887)
                     /locus_tag="VH752_000994"
     CDS             complement(6447..6887)
                     /locus_tag="VH752_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289356.1"
                     /translation="MSSRYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITISRALQPVPDKTSNDTPKESSNTIVKIIEKESSQSNQNHTGTSETTDDNKTTLP
                     SSSTAPSHNETTPTAPETPSTTEGTQLPNATGAEDANTGLPTKP"
     gene            7122..8264
                     /locus_tag="VH752_000995"
     CDS             7122..8264
                     /locus_tag="VH752_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ8289357.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNSLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALS
                     AYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGS
                     LVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            8276..9490
                     /gene="thiI"
                     /locus_tag="VH752_000996"
     CDS             8276..9490
                     /gene="thiI"
                     /locus_tag="VH752_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEQ8289358.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAIAVR
                     SDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTKITPEIV
                     NDEVENLIDALL"
     gene            9528..10820
                     /locus_tag="VH752_000997"
     CDS             9528..10820
                     /locus_tag="VH752_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEQ8289359.1"
                     /translation="MILLKSLYKGFFAAMKEENFEARCYNREKGGCVLIKTMQYKKTM
                     ATVVALIVALLLFGLIYDLLGVQKNKLAAQKSAQPKVKTARVVANGDILIHDILYMSA
                     RKADDTYDFTPYFEYVKDWISGADLAIGDYEGTISPDYPLAGYPLFNAPEEIAGALKN
                     TGYDVVDLAHNHILDSQLDGALNTKKVFHQLGIDSIGIYDKDRSKESFLIKNVNGIKI
                     AILGYSYGYNGMEATLSQEDYEKHMSDLDEAKIKKELQLAEKKADVTIVMPQMGTEYA
                     LEPTAEQKELYHKMIDWGADVVLGGHPHVIEPSETVIKGRQKKFIIYSMGNFISNQRL
                     ETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAHPTMVLAKGKGIVGKEGFELYNYRTM
                     VLEDFIKGGKYHDKIDEETKAKVALAYQEINDLVNLKW"
     misc_feature    10925..10999
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            11034..11348
                     /gene="rplU"
                     /locus_tag="VH752_000998"
     CDS             11034..11348
                     /gene="rplU"
                     /locus_tag="VH752_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEQ8289360.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            11360..11686
                     /locus_tag="VH752_000999"
     CDS             11360..11686
                     /locus_tag="VH752_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEQ8289361.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            11714..12007
                     /gene="rpmA"
                     /locus_tag="VH752_001000"
     CDS             11714..12007
                     /gene="rpmA"
                     /locus_tag="VH752_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEQ8289362.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            12355..13269
                     /locus_tag="VH752_001001"
     CDS             12355..13269
                     /locus_tag="VH752_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ8289363.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLNDIQGLPAVRFTQERDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            13266..13724
                     /locus_tag="VH752_001002"
     CDS             13266..13724
                     /locus_tag="VH752_001002"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEQ8289364.1"
                     /translation="MKKRLFVLSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMVHLDFVDFAIFNVADSYLTVGVILLVICLWKEEDYGN"
     gene            13714..14604
                     /locus_tag="VH752_001003"
     CDS             13714..14604
                     /locus_tag="VH752_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ8289365.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDALAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGHGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
     gene            15000..15521
                     /gene="pyrR"
                     /locus_tag="VH752_001004"
     CDS             15000..15521
                     /gene="pyrR"
                     /locus_tag="VH752_001004"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEQ8289366.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDIKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
     gene            15537..16796
                     /locus_tag="VH752_001005"
     CDS             15537..16796
                     /locus_tag="VH752_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEQ8289367.1"
                     /translation="MKDVIYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKAIGNDWIDNILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPLLLGLLVGYVVALLVGVLTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKIFSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            16857..17792
                     /locus_tag="VH752_001006"
     CDS             16857..17792
                     /locus_tag="VH752_001006"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEQ8289368.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGHFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKEGYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            17836..18918
                     /gene="carA"
                     /locus_tag="VH752_001007"
     CDS             17836..18918
                     /gene="carA"
                     /locus_tag="VH752_001007"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEQ8289369.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLS
                     NGPGNPEDLPEALDMIRGVQGKIPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVR
                     EIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDA
                     APGPHDASYLFDEFLEMIDSWRCTSK"
     gene            19164..22340
                     /gene="carB"
                     /locus_tag="VH752_001008"
     CDS             19164..22340
                     /gene="carB"
                     /locus_tag="VH752_001008"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEQ8289370.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPALDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQELQMHVDHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLREYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSVLIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLAIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSQLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GVPLFTALDTAKAMLTVLESRCFNIEAI"
     gene            22546..23817
                     /locus_tag="VH752_001009"
     CDS             22546..23817
                     /locus_tag="VH752_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ8289371.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGVVLWKQQQNTLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNKDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGAGSSTGAAYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVAVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            23817..24527
                     /locus_tag="VH752_001010"
     CDS             23817..24527
                     /locus_tag="VH752_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289372.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEILNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            24539..>24900
                     /locus_tag="VH752_001011"
     CDS             24539..>24900
                     /locus_tag="VH752_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289373.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNA"
BASE COUNT         7819 a         4232 c         5350 g         7499 t
ORIGIN      
        1 tcttttgtcg aaaagaagct gacagcaggg gtacaattat tagatgaaaa agatttttct
       61 aatataaagg aaaaaaatca gttggttcaa ctggtgacca agtccaatcg tcccattgga
      121 acagcatata tctctaagca aaataaagga attggttggt atctaggacc agaaaaaatc
      181 gacttgtcta tctcttattt tgttagtcta ttttcagttg ctaaggccaa aagacaagat
      241 tttgctcaat cggacgagac caatgcttat cgccttttta atcaagaagg agacggtttt
      301 ggtggagtaa caattgatct ttataaagat tttgcagtct tttcatggta caatgccttt
      361 gtctatgaca aaaaagaaat gattatggag gcttttcaac aggtttttcc tgaagtcaaa
      421 ggagcttatg aaaagtgtcg ctttaaaggt ccagatactg aaactgctca tctgtatggt
      481 gagctggcac aagaaacctt ttcgatttta gaaaatggca tagcctatca agtcttcttg
      541 aatgagggct taatgacagg tatttttcta gaccagcatg atgttagaag agcactagta
      601 gatggtttag ctatgggcaa gtcccttctt aacctatttt cctatacggc tgccttttct
      661 gtagcagctg ctatgggtgg tgctatcgag accacttctg tggacttggc taaacgatcg
      721 agagaattat cactagctca ttttgagcat aaccagttaa acttagcctc acatcatttt
      781 gtggtgatgg atgtttttga atacttcaaa tatgccaaac ggaaaaaatt aatctttgat
      841 gtgattgtga ttgacccacc aagctttgct agaaataaaa aacaaacttt ctctgtgagc
      901 agggactacc acaaattaat cacagaggcc ttggacatat tgagtcctaa gggaactatt
      961 atagcttcaa caaatgctgc taatatgact gttagccagt tcaaaaagca aattatcaaa
     1021 ggatttggaa gtcgtcgtcc tgagtctatg actctccagc agcttccgag tgactttact
     1081 atcaataaag cagatgaaag aagtaactat ttaaaggtat ttactataaa ggtacgagaa
     1141 tgagaattgt agcaccagtc atgccaagac attttgacga ggctcaagcc attgatattt
     1201 ctaaatatga agatgttaat ttaattgaat ggcgagcaga ctttcttccc aaggatgaga
     1261 ttgtcgcagt agcaccagct atttttgaaa aatttgccgg aaaagaaatt atctttaccc
     1321 ttcggaccgt tcaagaaggg gggaatatta ccctttcaag tcaggagtat gttgatatta
     1381 tcaaagaaat caatgctatt tataatccag actatattga ctttgagtat tttacgcaca
     1441 agtcagtatt tcaagaaatg cttgattttc caaatcttat tttgtcttat cacaactttg
     1501 aagagactcc tgaaaattta atggaagctt tttcagaaat gaccaagttg gcaccgcgtg
     1561 tggttaaaat tgctgtgatg ccccagagtg aacaagatgt tctagatttg atgaactata
     1621 ccagaggctt taagacgctc aatcctgagc aagaatttgc aactatatct atggggaaat
     1681 taggaagatt gtctcgtttt gcgggagatg tcattggctc ttcctggaca tacgtttcat
     1741 tagatcatgt tagcggtcca ggtcaagtga ctctcaatga tatgaaacgt attattgagg
     1801 tcttagaaat ggatatctct aactagtgaa tggtctaaaa atattatttc tacaatagtt
     1861 aagaaatgat gtttttagat taatgttttt aaaataagtg gaaccaaaaa ggagaaaaat
     1921 tgcgttattt aacagcagga gaatctcatg ggccatcctt aacagctatt attgaaggta
     1981 taccagcagg attaactctt catccagcgg atattgatca cgaattacag cgacgtcaag
     2041 gaggttatgg ccgtggtgcc agaatgtcta ttgagactga tcgggttcaa atatcctcag
     2101 gagttcgtca tggcaaaaca acaggggccc ccattacctt aacggtaatt aataaggacc
     2161 accaaaaatg gctagatgtc atggctgtag gagacattga agagactctc aagttaaagc
     2221 gtcgtgttaa gcatcctaga ccaggacatg cggatttggt aggaggtatt aaatatcatt
     2281 ttaacgattt aagagatgct ttagagcgtt cctcggctag agaaacaacg atgagagtgg
     2341 ctgtaggcgc tgttgctaaa cgtatcttag ctgaacttgg aattgatatg ttacatcata
     2401 tcttaatatt tggtggtata acgatcacaa taccttctaa gctctctttt agagagttgc
     2461 aagaaagggc tctccattca gaattatcta ttgttaatcc aaagcaagaa gaagaaatta
     2521 aaacgtatat tgataagata aaaaaagaag gggatactat tggtggtatt atcgaaacga
     2581 tagttcaagg ggtgccagca ggtcttggat cttatgtcca atgggataaa aaattagatg
     2641 ccaagctggc tcaagcagtt ctttccatta atgcttttaa aggggtggaa tttggagttg
     2701 ggtttgatat gggattccaa aagggctctc aggtaatgga tgaaatcacc tggacaccta
     2761 cccaaggcta tggtcgtcaa acaaaccact taggtggctt tgaaggtggc atgaccacag
     2821 gacaacctct tgttgttaaa ggggttatga agcccattcc tactttgtat aaaccactca
     2881 tgtcggtaga tattgatagt catgaacctt ataaagcaac ggtggaacgc tctgatccga
     2941 cggccttacc agctgcaggg gttatcatgg aaaatgtggt ggcgacggtg cttgccaaag
     3001 agattttaga gactttttct tctactacca tgtctgaatt gcaaaaagct ttttcggact
     3061 atcgtgccta cgtcaagcaa ttttagatag cgagatactg gcttttatgg tatcatacag
     3121 gtattaatga acaaaaggag actgtcatgt cacaagaaat ttatgactat gctaatcaac
     3181 ttgaaagagc agttcgtgct ctgccagaat accaaaaagt gttagaagta aaagaggcca
     3241 ttcaagcaga cgctagtgct agcgaactgt ttgatgagtt tgtcgctatg caagaaaaaa
     3301 tccaagggat gatgcaatca ggccaaatgc caacggctga agaacaaaca agcattcaag
     3361 aacttagcca aaagattgaa gctaatgacc agctaaaagc ttattttgaa gcgcagcagg
     3421 ccttatctgt ttacatgagt gatattgagc gtattgtatt tgctccgttg aaagatttag
     3481 tcaagtaagc caattaagat aggaaaaaaa cagattgctg gcagctcgtc aggatctgtt
     3541 ttttagtgtt tattttcttt gtatcagaaa gttgagcctc tttcttattt gtgatatagt
     3601 caaataaagt gaccaaaata gcaggccagt agtggcttta atgaaggcaa gttgtctgct
     3661 aactgactat gctaaaacat cagtattaga aagtaaaagg tgataaaaat ggttattcct
     3721 tatgattata ttgttattgg tggagggagt gcaggtattg cttctgcgaa cagggcagcc
     3781 atgcatgggg ccaaagtatt gttagctgaa ggtaaaaaga ttgggggaac ctgtgttaac
     3841 ctaggctgcg ttcctaaaaa agtcatgtgg tatggcgctc aagtagcaga tattttaggg
     3901 acttacgcta aggattatgg atttgacttt aaagagaaag catttgattt taaacaattg
     3961 aaagcaaatc gtcaagctta tattgatcgg attcatgctt cttatgagcg aggctttgag
     4021 cagaatggtg ttgaccgtat ttatgattat gctgttttca aagatgctca tacggtagaa
     4081 attgcagggc agctttatac cgcaccgcat attttgattg ccacaggagg acatcctgtt
     4141 tttccagata ttgagggggc acaatacggt atcagctcgg acggcttttt tgccttagat
     4201 gaggtaccaa aacgtacggc tgtggttggg gcaggctata ttgctgtgga acttgccggt
     4261 gttttgcatg ctttggggtc gaagacggat ttgtttatcc gtcacgatcg acctttaaga
     4321 agttttgaca aagccattgt ggatgtttta gttgaggaaa tggcagttgg tggtccttgc
     4381 ttacaccctc attcagaagt ggcaaaggtt gtcaaaaata ctgatgagtc cttgaccctt
     4441 tacctcaaag atggccaaga agttgaggtt gaccagctca tttgggctat cggtcgcaag
     4501 cctaatctag aaggttttgg tcttgacaaa actggagtga cgttaaatga taaaggttat
     4561 attgagacag atgcctatga aaacacgtct gtcaaaggta tttatgccgt gggagatgtt
     4621 aatgggaaac tggctttaac tccagttgct gtggcagctg gacgtcgcct atcagagcgt
     4681 cttttcaatg gcaaaataga cgagaaattg gactaccaaa acgttgctac tgtgattttt
     4741 agtcaccctg ttattggctc tgttggcttg tcagaagaag cagcagtcaa acagtatggg
     4801 caagaagcag ttaaaacata tcaatcacgt tttacgtcta tgtttactgc tatcactaac
     4861 catcgccaac cttgtctgat gaagttggta acagtcggag ataccgaaaa aatagttggt
     4921 cttcacggaa tcggctatgg ggttgatgaa atgattcagg gctttgcagt agccatcaaa
     4981 atgggagcaa ccaaggcgga ttttgataac accgttgcta ttcacccaac tggctcagaa
     5041 gaatttgtca ccatgcgcta aaaaatgtag cctaaggcaa taaaaaatga tgttaacagt
     5101 ttttgaggaa agtcttctaa actgctagca tcgttttttg ttaggtgatt aggggaatat
     5161 gtttttaaag atatttgcgt tgctcagaca atattgcctt acctcggaga taaaataaag
     5221 tgaccctgta acaacaaaga gttcatcaga tgttacggaa ccttgtaacc agtctctgta
     5281 atcttttacc tgtgggtatt tatcaggata atcctctaaa agatttgctt cagggaaatc
     5341 aaaagtcgtg acagatactt caaaagtatc tagttgagcc agcaagtcgg ctaaaggctt
     5401 acgtttgaga cctgcaaaga gaatatgaat aggacgtttg ggaaattctt cttgcagtag
     5461 agactttaat ttagcaatgg aatctggatt gtgagcgccg tctaatagaa gattaggttg
     5521 aataaattcg gaccggccag gccaagttgt tgcctcaatt cctttttgaa tcgtttttgg
     5581 agatatttta ggaaaggatt ttctaagtaa ttgagctgtc ataatggcta aacaggcatt
     5641 cgctttttga tgttgaccta atagtttgag acggagtttg gttaaattca agttatcgtg
     5701 ttgaaaggaa aacgtctttt ctgaaggatt gagcaaaaaa tcttttccta attgatataa
     5761 gcgtgaaccg gctttttggg taacttgttg aaagacttga ctagcctcct gctcaagttg
     5821 accgataata acgggttctt tagctttgat aacttctgct ttttggtgag caatttctgc
     5881 taggctataa cctaaacgtt cttggtgatc aaaactaata gaagggcata cgacggccag
     5941 agcatgaaaa acattggttg cgtcggtttt cccaccaatc cctgcctcaa taatggcaat
     6001 atcaacaggt ttaagattgg caaagtagca aaacattatg agcgtcacta actcaaactc
     6061 ggtaggtcta tcccaatttg tttcaaggga cattttagtg agaatgggtc taatcacgct
     6121 cacacattgc gccaattcta ggtctgaaat gggctggcca ttaaggcaaa tccgatcgtg
     6181 aaaagagata ataaaaggag atgtaaaggt tccgacctga taaccagaag ttgacaggat
     6241 atgctgtaag taggctgtca cagagccctt gccgttagtt ccaacaacgt gaatagcagg
     6301 gaatttgtct tgtggccggc ctagtgcctc aagtaaccaa gccattctgg ccaaatcggt
     6361 cttgcgacca ttagccttaa aggtatgaat ccaggaaatg ctatcatagg tagtcattac
     6421 tatttcttgt ccttctaaca atgcttttaa ggtttcgtag gaagaccagt attagcatct
     6481 tcagctccag ttgcattagg aagttgcgtc ccttcagtgg ttgatggtgt ctcaggagct
     6541 gtaggagtag tctcgttgtg agaaggtgcg gtagaagatg acggaagagt tgtcttatta
     6601 tcatctgtgg tttctgaagt acctgtatgg ttttgattac tctgagaaga ctctttttca
     6661 ataatcttga caatggtatt actcgattct ttgggtgtat cattagaggt cttatctggt
     6721 acaggctgca gtgctcttga aatggtaatg gttgccacga tgatactcaa aatactacca
     6781 ataagtgcaa tggatttttg gaaagctgta aaacctgttt taatatgctc ggtagggaca
     6841 cctcctgatt tacgaggttt ttgttgacgg gaataacgtg atgacatatt tttctccttt
     6901 aaaatcatcg taatgccaag attaaggagg agtaaacaga agtcctagct cctctttaac
     6961 acttcatcta tcagtataat ctagaaaaaa agtgaagtca acggtttacc ttatgtctag
     7021 gacaaggaag tcctttgggc ttattgtaat acgaatattc ttgatttcat tttagctatt
     7081 ttgtgataga ataaagtatt gagtaaaaat gaaggtgaca catgatttat tttgataatg
     7141 cagccacaac cataccttat ggagaggctc ttaaaactta ccaagaagta gcaacaaaaa
     7201 tatatggcaa tccctcaagt ctgcaccaat tagggacaaa tgcgtctcgc attttagaag
     7261 cttcccgtaa acagattgct ggtttgttgg gtgttaagtc agaagaaatc ttttttacgt
     7321 ctggtgggac tgaaagtgct aattgggcta ttaagggaat tgcttttgaa aaaactgctt
     7381 ttggcaaaca tattattatc tcagccattg aacaccctgc cgtgagtgaa agtgtcaaat
     7441 ggcttctcac acaaggattc gaagtttctt atgctccagt gacaacacag ggagttgttg
     7501 atgtgaatgc tttagctgag ctgattagac cagacaccat cttaatctcg attatggctg
     7561 ttaataatga aatgggagct atccaaccga ttagagccat ttcaaaccta ttggctaacc
     7621 aaccaacgat cacctttcac gttgatgctg ttcaagccat tggtaaaata ccactctgtg
     7681 attatatgac taatcgtgtg gatttggctt ctttttcagg gcacaaattt catagtgttc
     7741 gtggtgttgg cttcctttat aaaaaagcag gcaagcgact caattctctc ttgtcaggcg
     7801 gcggccaaga gcaggagtta cgttcaacca ctgaaaatgt tgcaggaatt gctagcatgg
     7861 ccaaagcatt aagaatagtc actgaaaaac aagtgagcgt cttacctaaa ctaacagcga
     7921 tgcgagatgt gatttacaaa gctttgtccg cttacccaga tgtgacagta ttttcagctc
     7981 aagaaggctt tgcgcccaat attttgacct ttgggattag aggggttcga ggagaagtta
     8041 ttgtccacgc ctttgaaaaa tatgagattt atatttcgac cacaagtgca tgttcgtcca
     8101 aagcaggtaa gccagcagga agcctagtgg ctatggggat tcctgtaaaa actgcccaga
     8161 cagccgttcg tattagtcta gacgatgaca acgatatggg acaagtagag cagtttttga
     8221 ccatattcaa acagatttat gataaaacac agaaagttag gtaagattag gtaagatgga
     8281 ctattcagaa attatggttc gccatggtga actgtcaact aaagggaaga atcgcatgcg
     8341 attcattaac aaacttaaaa acaatattca agacgtactg gctcctttcc cagccattgc
     8401 tgtccgttca gatcgtgata ggacccatgt gtctctaaat ggcacagatt atcagcctat
     8461 tgtagaagct ttaaagctgg tgtttggtgt tcaagctcta tctcctgttt ataaacttga
     8521 aaaaagtgtc cctcttttgg taacagctgt tcaagatatc atgacttctc tttatcgtga
     8581 tggactgacg tttaagattg ccactaagcg tagtgatcac gcgtttgaat tggatagtcg
     8641 tgagcttaac agtcttcttg gtggagctgt ttttgaggtt ttaccaaata tccaagccca
     8701 gatgaaacat cccgatgtga ctctcaaagt cgaaattcgt gatgaagcag cttacatttc
     8761 ttatgaagag attaagggag caggcgggtt accagtagga acgtcaggga aagggatgct
     8821 gatgttatcg ggcggtattg attcaccagt agctgggtat ttagccctta aacgtggcct
     8881 cgatattgaa gttgttcatt ttgctagccc tccgtacacc agtccaggtg ccttggctaa
     8941 agcccaagac cttactcgtc ggttaactcg ttttggtgga aatatccaat ttatcgaggt
     9001 tccttttaca gaaattcaag aagaaattaa aaataaggca ccagaggctt accttatgac
     9061 attaacccgc cgctttatga tgcgcatcac agatgccatt cgtgagcaac gtaagggtct
     9121 tgttattgtt aacggtgaga gcctaggtca ggttgctagt caaacccttg aaagcatgca
     9181 ggccattaat gcggtaacat caaccccaat tattagacca gtcgtgacaa tggataaact
     9241 tgaaattatc gagatggctc aggcgattga tacttttgac atttctattc aaccgttcga
     9301 agattgctgt actatttttg caccagaccg tcctaaaacc aaccctaaac taggtaatgc
     9361 tgaaaaatat gaagaacgct ttgatattga tggtcttgtc cagcgtgctg tgtcaggaat
     9421 tgttgtcaca aaaatcacac ctgaaatcgt caatgatgag gttgaaaatc tcattgatgc
     9481 tttactttaa aagaaaagat caagtgtaaa tcttttagta gtcagtaatg atactattga
     9541 aaagcctcta taagggcttt tttgctgcaa tgaaggagga aaattttgag gcaagatgtt
     9601 ataatagaga aaaaggaggg tgtgtcttga ttaaaactat gcaatacaaa aaaacgatgg
     9661 caacagttgt tgctcttatc gtcgcacttt tgctatttgg cttgatttat gatttacttg
     9721 gggttcaaaa aaataagcta gcagctcaaa aatctgctca gccaaaggtc aaaacagcgc
     9781 gtgttgtagc taatggcgac attttaattc atgatattct ctacatgagt gctagaaaag
     9841 ctgacgatac ctatgatttc actccttatt ttgaatatgt taaggactgg attagcggtg
     9901 ctgacttagc tattggagat tatgaaggga ccattagccc agattatccc ttagcaggct
     9961 atcccttatt caatgctcca gaagagattg caggtgctct gaaaaatact ggctatgatg
    10021 tagtcgattt agcccataat catatcttag attcccaatt agacggtgcc cttaacacta
    10081 aaaaggtctt tcatcaatta ggtatagaca gtattggcat ctatgacaag gatcgttcaa
    10141 aagaatcctt cttaattaaa aatgtcaatg gtatcaaaat tgcaatttta ggttattcgt
    10201 atggttataa cggcatggag gccacactta gccaagaaga ctatgagaaa cacatgtctg
    10261 atttagatga agctaagata aaaaaagaac ttcagctagc tgaaaaaaag gctgacgtga
    10321 ccattgtcat gccacaaatg ggaacagaat atgccttaga accgacagca gagcaaaaag
    10381 aactttacca caaaatgatt gattggggag ctgatgtcgt cctaggaggc catccgcatg
    10441 ttattgaacc ttcagagaca gttataaaag gtaggcaaaa gaaattcatt atttactcta
    10501 tgggaaattt tatttcaaat cagcggcttg aaaccgtaga tgatatctgg actgagcgtg
    10561 gcctattgat ggaccttact tttgagaaaa aagataacaa aacaaaaatt aaaacagttg
    10621 aggcccatcc aaccatggtt ttagccaaag gtaaaggcat tgtgggtaaa gaaggttttg
    10681 aactatacaa ttatcgaaca atggttttag aagattttat caaaggtgga aaataccacg
    10741 acaagattga tgaggaaacc aaagcaaaag tagcacttgc ctatcaggaa attaatgatt
    10801 tagttaacct gaaatggtaa tgtttggtgg tttagcatct aacgataaaa aatagagatt
    10861 aaacttaatt tttccttgat aaaaagcagt tatggtgcta aaatagtaac gttgactaaa
    10921 tgcacatcct gtgcaaccgc acgaaaatcg ttgtcaagta gaccaagtct cacgattcca
    10981 ggcgagtctt cacagaggga aacctcaact aaaaatttta taggaggtgc atcatgagca
    11041 catacgcaat catcaaaact ggtggaaaac aagttaaagt tgaagtaggt caagcaatct
    11101 acgttgagaa aattgacgct gaagctggcg cagaagtcac ttttaacgaa gttgttcttg
    11161 taggtggtga caaaactgta gttggtactc cagttgttga aggagctact gtcgttggaa
    11221 ctgttgaaaa acaaggaaaa caaaagaaag ttgtaacttt caaatacaaa cctaaaaaag
    11281 gtagccaccg taaacaaggt catcgtcaac cttacactaa agttgtcatc aacgcaatca
    11341 acgcttaatt tactagctta tgattaaagc aatttttact cgccaaaaaa atggtcaact
    11401 gagcagtgtt acactgacag gtcatgctgg tagtggtaaa catggctttg atattgtttg
    11461 tgcctctgtt agtactctag ccattaactt tgttaattct cttgaggtgt tagcagattg
    11521 ccaagcatta gtagatctta atgatgttga aggtgggtat atggctatta caataccacc
    11581 acatgacaac aaggaggaag ttcaattatt atttgaatct tttcttctag gaatgactag
    11641 tttggcgaag gattcttcga aatttgtcaa cactcaggtc atctaaattt aatttaagag
    11701 aggaaacaac attatgttaa aaatgaatct tgctaacttg caacttttcg cccacaaaaa
    11761 aggtggaggt tctacatcaa acggacgtga ctcacaagct aaacgccttg gtgctaaagc
    11821 tgctgacggt caaactgttt caggtggatc aatcctttac cgtcaacgtg gaactcatat
    11881 ctacccaggt gtaaacgtag gccgtggtgg agatgacaca ctttttgcta aagttgaagg
    11941 tgttgtacgt ttcgaacgta aaggacgcga taaaaaacaa gtttctgttt acccagtagc
    12001 taaataattg tttaacatcg cattatataa ggctttccag gtgtctggaa agccgatttt
    12061 tttattttgc tactcatttg ctacccatat tctaaaagcc gatgtaggaa gagagcttgt
    12121 cagcagtttt atcttttgag gatttagtaa catgagcgta gatattcatg gtagtttgga
    12181 tagtttcacg accaagtgta ttggattttc ttctatccta ctaaagattg tagttgctta
    12241 tttagagcaa tagttctaat tgtaaaaagt cagcataata aaacaatatc ataagatgtt
    12301 gttataacgg gctttatggt ataattaagg atactcattt taggaaggaa agcaatgaat
    12361 attcaacaat tacgctatgt ggtagccatc gctaataatg gaacttttcg agaagctgcc
    12421 tctaaattgt ttgtcagtca gcctagctta tcagtttcta tcaaagattt agaggcggaa
    12481 ttagggtttc aaatttttaa tcggactact tcaggaacag tcttgacaag tcaaggacta
    12541 gttttttatg agaaagcttt agaagtagtc aaatcttttg attcttttga aaaaaccttt
    12601 tctcaagctg atctggatca aaatgaattt tctattgcta gccaacatta tgactttttg
    12661 ccacctctta ttacagcttt ttcgcaacaa tacgatggtc atcgtgtctt tcgtatcttt
    12721 gaatcaacaa ctattcaaat tttagatgaa gttgctcaag gaaacagtga aattggtatt
    12781 atttacttga atgtagacaa tcaaaaaggt ctctttcagc ggatggataa gctgggctta
    12841 gaatatgttt ctctaattcc ttttactact catatttatc tttcaaaaac gcatccattg
    12901 gctaatcgcg aagcgcttta tttgaacgat attcaaggat tacctgcggt aaggtttacc
    12961 caagaaaggg acgagtattt gtattattct gaaaattttg tggatactag tgaatgccct
    13021 cgtatttata atgtgtcaga tagggcaacc ttgaacggta ttttagagag aaccaatgcc
    13081 tttgcgacag gatcaggttt tctcgatcat cggagtgtta atgggattaa agtgatacct
    13141 ctagctgatc atattgacaa tcaaatgatt tatgtcaaac gaaaagataa aaacctttct
    13201 gttgctgggg ccacttttgt gactatttta aaagactatt ttgataaaag aaggaaggcc
    13261 ttaacatgaa aaaacgattg tttgtgctta gcttgatcct ccttgtagct ttggatcaac
    13321 ttagtaaatt ttggattgtt tctcatatag cgcttggaga agtgaaaccc tttatcccag
    13381 gtatcgtcag cttgacttac ttgcaaaaca atggggctgc cttttccata ttgcaggacc
    13441 agcaatggtt ctttgttgtc ataacggttt tagttatcgg ttatgctatt tattaccttg
    13501 ctactcatcc ccatttaaat atctggaagc aattagctct cttgcttatt atttctggtg
    13561 gaatcgggaa ttttattgat cgtttgcgtt tagcttacgt gattgatatg gttcatttag
    13621 actttgtgga ttttgccatt tttaatgtgg cagattcata ccttaccgtt ggtgtcatat
    13681 tgttagtgat atgtttatgg aaagaagagg attatggaaa ttaatgtgat aacatcgggg
    13741 caacgcttgg ataaggcact agctgattta agtcccctgt cacgtggtca agccaatgac
    13801 caaattaagc aaggccttgt tttagttaat ggtcagcaaa aaaaggctaa atatacggtt
    13861 caagcaggag atgtgatttg ttttgaactc cctaaagaag aagtcttaga gtaccaagct
    13921 cagaatattc ccttggacat tatctatgaa gatgatgctc tcgccattat taacaagcca
    13981 cagggaatgg tggttcaccc ttcggcagga cacccttcag gaacaatggt taatgccttg
    14041 atgtatcata tcaaggattt gtcctcaatc aatggtgtgg ttcgtccagg aattgttcat
    14101 cggattgata aggacacctc aggtctctta atggtagcta aaacagatgc agctcatcaa
    14161 gccttggcag aagaattaaa ggctaaaaaa tctctcagaa aatacctcgc cattgtccat
    14221 ggaaacctac cgaatgatcg cgggatgatt gaagctccta taggtcgtag cgaaaaggac
    14281 cgtaaaaaac aggctgttac agcaaaaggc aaagaagcag tgacacgttt tacagtgcta
    14341 gaacgttttg gcgattacag tctagtggaa ttacaactgg aaactggtcg aacgcaccaa
    14401 attcgtgttc atatggccta tattggtcat cctgttgctg gggacccttt atatggacca
    14461 cgtaagacac tatcaggtca tggccaattt cttcatgcaa aaacattggg attaacgcat
    14521 cctatgacag gaaaagaaat gatatttact gttgaagctc ccgaaatttt tcaaaaggtg
    14581 ttaaagctgc taagaaaaaa ataaacagcg aggagtagcc attattttta gtcttatatc
    14641 tttgtgcaaa gtttttaaca ttataaaaaa tactaaaaag catttacgat aagccaggaa
    14701 atatgagaag tcttttatgt tattgggata tgcaacatat ctgttagaga tctttattta
    14761 aactccttaa agaaaagaga tattccaaat agatgctagg tattgtcttt tttacttgtt
    14821 tttcttttct ttttttgcta tactgattga aataaaatat tcctttaaaa cagtcctgtg
    14881 aggctgtcaa ggagacggat taaaaacatg caccatgata cttaattgtg ctatattttg
    14941 ctgaatctcc ttgtttccaa ggagatcttt ttatttgaat ttttagagga ggtttcaaca
    15001 tgaaaactaa ggaaattgtt gatgacgtga ccatgaaacg cgccattacc cgtattactt
    15061 atgaaattat tgaacgtaat aaacaattag acaatgtggt tttggcaggc atcaaaacga
    15121 gaggtgtttt tctagctcgt cgcattcaag aaagactgca tcaattagaa gggttggatt
    15181 taccaattgg tgagttagac atcaagcctt ttagagatga catgagagtt gaggaagaca
    15241 caactctcat gtcagtagat attacaggca aggatgtgat tttgattgat gatgtgcttt
    15301 atactggtcg taccatccgc gcagccatcg ataacttagt gagtttaggt cgaccagccc
    15361 gtgttagtct agctgttttg gtggatcgtg gccatcggga attaccgatt cgcgcggatt
    15421 acgttgggaa aaatattcca acaagcagtg tagaagaaat tgttgttgag gttgtcgaag
    15481 tcgatggccg tgatcgtgtt agcattattg acccaacata agaaagaggt tttactgtga
    15541 aagatgtcat ttatgatgtg gaagaagtgc caaaggcagg tatgttagtt ggtttgtcct
    15601 ttcagcactt atttgctatg tttggtgcga ctgttctggt tcccattcta gttggaattg
    15661 acccatcagt tgctttgtta tctagtggtt taggaaccct tgcccactta tcagtgacca
    15721 aatttaaaat tccagcctac atgggctcaa gttttgctta tatagcagct atgcagttac
    15781 tgatgaagac aaatggtatt ggtgctgtag ctcaaggggc tatgacagga ggtctcgttt
    15841 acctgattgt agctttgatt gttaaagcta ttggcaatga ttggattgat aacatcttac
    15901 caccgattgt ggttggtccg attgttatgg tcattggtct aagcctagct tctacagctg
    15961 ttaatgatgt catgctaaaa aatgggaact ataatctgac ttatcttgtt attgggttag
    16021 tcaccttatt atcagttatt tttttcaata tttatggtaa gggaattgtt gctattgtac
    16081 cgcttttatt agggctatta gttggttatg ttgtagccct tttagtgggt gttctcaccg
    16141 gtcaagaaat tgttgatttt accaatgtgg ctcaggccaa atggtttagt atcccatcag
    16201 tggaaattcc tttcttaacc tacggggtta aattctaccc aagtgccatt ttaactatgg
    16261 cacccattgc ctttgttaca atgacagaac attttggaca cattatggtc ttaaatagtt
    16321 tgacaaaaag agattatttc aaggatccag gacttgaaaa gactttaact ggtgatggtt
    16381 ttgcgcaaat cattgccggc ttcttagggg cacctccggt cacttcttat ggtgaaaata
    16441 tcggtgtaat ggctttgaat aaaatattct cggtttatgt tattgcaggt gccgcagtga
    16501 ttgccgctct cctcagtttt atcggtaagg tatcagcctt aattcaatct attccaacac
    16561 cagttattgg aggtatatca gttgccttgt ttggtgtaat agcttctagc ggtttgaaaa
    16621 tcttgattga atccaaagtt gatatggata ataagaaaaa cttattgatt gccagtgtta
    16681 ttttggtctc tgggattgga ggattgatgc ttcaagtaaa tggtcttcaa atttcaggtg
    16741 tcgctttttc aacacttctt ggcatcatct tatatcaggt acttcctgaa aaataggaag
    16801 aattgttgtt agaccactta catagtcatc agtcgttagt gataaagggg ataatcatgt
    16861 cagttgtaaa taatcgcgtt gcacttacca atttagtttc aatggaagca ttaaccaccg
    16921 aagaagtttt aggtcttatc aaccgtggtt cagaatataa ggctggtaag gttgttattt
    16981 cggatcatca aaaggatctt gttgccaatt tattctttga aaactccacc cgaacccata
    17041 agtcttttga agtggctgag aagaaactcg gcttgacagt tcttgatttc aatgctgatg
    17101 caagtgcggt taacaaggga gaaagtttgt atgatactgt gttaaccatg agtgcacttg
    17161 gaacagacat ctgtgtgatt cgtcacccag aagatgatta ttacaaggaa ttagtagaaa
    17221 gtccaactat tacagcttcc attgtcaatg gaggagatgg ttctggacaa cacccgagtc
    17281 aatgtttact tgacttatta accatctatg aagaatttgg acactttgaa ggcttaaaga
    17341 ttgcgatcgc tggtgactta actcactcta gggtggcaaa gtccaacatg caaatcctca
    17401 aaagattagg agcagagctt tatttctatg gacctgaaga atggtattca gaagccttta
    17461 atgcttacgg cacttatatt gctattgacc agattattaa ggaattggat gttcttatgt
    17521 tgctgcgtgt acagcacgaa cgtcatgacg gccatcagag tttttcaaaa gaaggttacc
    17581 atcaagcgtt tggattgaca caggaacgtt accaacaatt aaaagattct gctattatta
    17641 tgcatccagc tccagttaat cgggatgtgg aaattgcaga tagtttggtg gaagctccta
    17701 aagcgagaat tgtatctcag atggctaatg gtgtctttgt tcgcatggct attattgaag
    17761 ccattttgaa tggtagaaat aaaaactcat gatgaaagat taaagcatgt ttagaaggta
    17821 ggaaaaggta ggaaaatgac aaaacgatta cttattttag aagacggcac tatttttgaa
    17881 ggagaaccct ttggtgctga tattgatgtg actggtgaaa ttgtctttaa tacaggaatg
    17941 acaggctacc aagaatccat cactgaccaa tcctacaatg ggcaaatttt aacctttacc
    18001 tatcctttga ttggaaatta tgggattaat cgagatgact atgagtccat ttcacccacc
    18061 tgtaaagggg ttgttgtgtc tgaagtcagt cggttggcaa gtaattggcg caagcaaatg
    18121 acgctagatg cgttcttgaa aataaaaggg attccaggga tttcgggaat tgatacgaga
    18181 gctttgacca aaattattcg gcaacacggt accatgaaag ctacgatggc agatgatgga
    18241 gatagtatcc agcatttaaa agatcaactg cgtgcaacag tcttaccgac taacaccatt
    18301 gaacaagtgt caactaaaac agcttatcct gcaccaggga tcgggaaaaa tattgtattg
    18361 gttgattttg gtttaaaaca ttctatttta agagaatttt ctaaaagaca atgtaatatc
    18421 accgttgtcc ctttcaacat cactgcagaa gaagtgctcc aactaaatcc ggatggcttg
    18481 atgctatcta atggcccagg aaacccagaa gatttgccag aggctttaga catgattcgt
    18541 ggggtacaag gtaagattcc tatttttggt atttgtatgg gacatcaact ctttagtcta
    18601 gcaaatggtg ccaagacttg taaaatgacc tttggtcatc gtggctttaa ccatgccgtt
    18661 agagaaattg caacgggaag aattgatttt accagtcaaa accatggtta tgccgttgaa
    18721 cgtagtagtc tacctgatac tttaatggtt acgcatgaag acattaacga taagacggtc
    18781 gaaggagtga aacatagaga ttttccagct ttttcagtcc aatttcaccc tgatgcggct
    18841 ccaggacctc atgatgccag ctacttattt gacgaatttt tggaaatgat tgattcttgg
    18901 agatgtacga gtaaatagta aatcagttgc aatttcacga ctatatcacc tcttgttact
    18961 ctatttatta acatttacag ttgatttagt accctatcaa aaatattatt taatgttgct
    19021 ctgtgaggca acaaatagag aaaggaaggc aaggagaaca caagtaaata atttagtaaa
    19081 aagttaggtt ttcaaagaga taaagctctt attgagaagt gctgacttta ccgcttcttg
    19141 tggtattctt ggtatcatta attatgccaa aacgaaaaga tattcaaaaa atcatggtta
    19201 tcgggtctgg cccgattatt attggacaag ctgctgaatt tgattatgca gggactcagg
    19261 cctgtttggc tctaaaagaa gaaggctata aggtcatact tgttaattca aatcctgcaa
    19321 cgattatgac agataaagaa atcgctgata aggtatatat tgagcctttg actcttgaat
    19381 ttgttaatcg tatcattcga aaagaaaggc cagatgccat actgccaacc ttaggtggtc
    19441 aaacgggcct caatatggca atggcattat ctaaagctgg tattcttgat gatttagaga
    19501 ttgaattgtt aggtactaag ttatctgcga ttgatcaggc agaagacaga gaccttttca
    19561 aacaattaat gcaagaacta gaccaaccca ttccagaatc tacaattgtt aaaacagttg
    19621 atgaagctgt tacatttgct agagatatcg gttatccagt cattgtgcgc ccagctttta
    19681 ctcttggtgg aactggtgga ggaatatgta gtagtgaaga agaactttgt gaaattactg
    19741 aaaacggcct aaagttatca ccggtcacac agtgtttgat tgaacgctca attgctggct
    19801 tcaaagaaat tgaatacgaa gtaatgcgag atagtgctga taacgcgctg gttgtttgta
    19861 atatggaaaa ctttgatccc gtagggattc acacaggaga ttctattgta tttgcaccaa
    19921 cacagaccct ttctgatatt gaaaatcaaa tgttacgtga tgctagttta aaaataattc
    19981 gagctctgaa aattgaaggt ggctgtaacg tccagttagc actagatcct tatagtttca
    20041 aatattatgt tattgaagta aatcctagag tgtctcgttc ctctgccctt gcttcaaaag
    20101 caacaggtta tcccattgcc aaattggctg caaagattgc tgttggccta acacttgatg
    20161 aaatgattaa ccctatcaca ggcactactt acgctatgtt tgaacctgcc cttgattacg
    20221 ttgttgctaa aattccgcgt tttccgtttg ataaatttga acatggtgaa cgccaactgg
    20281 gaactcaaat gaaagcgaca ggtgaagtca tggctattgg ccgtaatttg gaagagtcct
    20341 tactaaaagc ctgtcgttct ttagaaattg gtgtttgcca taatgagatg acaagtttaa
    20401 gtaacattag cgatgaagag ttagtgacta aagtgattaa agctcaagat gatcgacttt
    20461 tttacttgtc agaagcaata cgacgaggtt atagtatcga agaacttgag agcttgacca
    20521 agattgattt atttttcttg gataaattac ttcatattgt tgaaatagag caagaattac
    20581 aaatgcatgt tgaccatttg gaaagcctca aaaaagcaaa gcgttatgga ttttcagatc
    20641 aaaaaattgc agaaatttgg caaaaggatg aaagtgatat cagagcgatg agacattctc
    20701 atagcctgta tcctgtctat aaaatggtag atacttgtgc agctgaattt gatgctaaaa
    20761 ccccttattt ttattcaaca tatgagttgg aaaatgaatc tgttcagtcg aataaagaat
    20821 caattttagt tttaggttca ggccctattc gtattggtca aggggttgaa tttgactacg
    20881 caacggttca ttctgttaaa gccatccaaa aagctggcta tgaagccatt atcatgaatt
    20941 caaatccgga gactgtatct acagattttt cggtatcaga taaattatat tttgaaccgt
    21001 taacctttga agacgtcatg aatgtgatag atttagagca gcctaaggga gtaatagttc
    21061 agtttggagg acaaactgct attaacttag cacaagcact ttctgaggcg ggtgttacaa
    21121 tattaggaac acaagtagag gatttggata gggctgaaga cagagattta tttgaaaagg
    21181 cgttgaaaga gcttggaatc ccacaaccac aaggtcaaac ggcaactaat gaagaagaag
    21241 ctttagaggc agctaaaaaa attggttttc cagtcttagt tagaccttct tacgtacttg
    21301 gtggccgggc tatggaaatt gttgaaaaca aagaagatct tagagaatat ataagaacag
    21361 cggttaaagc aagtcctgaa catcctatct tagtggactc ttatattttc ggtaaggagt
    21421 gtgaagtgga tgccatttca gatggaaaat cagtcttaat tcctggtatt atggagcaca
    21481 tcgaaagagc aggtgttcat tcaggagatt caatggcagt ttatccgcca cagcaattat
    21541 ctaagcaaat tcaagaaaca atagctgagt atactaaacg tttagccatt gggttaaatt
    21601 gtattggtat gatgaatgtt caatttgtta ttaaaaatga acaagtttat gttattgaag
    21661 tgaatccacg cgctagtcgt accgtacctt tcttatcaaa agtaacaggt attccgatgg
    21721 ctcagattgc tactaagttg attttaggtc aaacattaaa ggatctagga tatgaagatg
    21781 gtctttatcc ccaaagtcaa ctggtgcata tcaaagcgcc tgtgttttca tttacaaagt
    21841 tggcacaggt tgatagtctt ttaggtcctg aaatgaaatc aactggagaa gtaatgggtt
    21901 cggatactag tcttgaaaaa gcactttata aagcgtttga agccaataat tctcatttat
    21961 cagaatttgg tcaaattgtc tttactatag cagatgatag taaggcagaa gctctaagtc
    22021 tagcaagacg atttaaggcg attggctatc aaattatggc tactcaaggt acagcagctt
    22081 actttgctga gcaaggctta agcgcgtgtt tagttggaaa aattggtgat gctgccaatg
    22141 acatcccgac actcgtgcga catggtcacg ttcaggcaat tgttaataca gttggaatta
    22201 aacgcacagc tgataaagac ggacagatga ttagaagttc agctattgaa caaggagttc
    22261 ctttatttac agctttagat actgcaaaag ctatgttaac ggttttagaa agcagatgct
    22321 ttaacatcga agcgatataa aaattaaaaa atcattttta agaaaactta aaaatgattt
    22381 ttttagatta taatgacaaa agtttcagta agctattaat ttgtcatagc atataagtca
    22441 ttatcttgtg gcttgttttc gactaagggc taagaatttc ttgacaacaa tataaaaagg
    22501 tcatatactt gtcttataca aatagtacaa aaggggagga tagaaatgtc taaaagagga
    22561 aaaattaaaa taacaacgaa aacaaagctt attacagcta gtgttataac gctagtatta
    22621 attataactg gagtagtctt gtggaaacaa caacaaaata cgctaacagc tgatatcgct
    22681 aaagaacctt actctactgt tagtgtaact gaagggagta ttgcttcttc gactttacta
    22741 tcaggtactg taaaggcttt atcagaggaa tatatttatt ttgatgctaa taaaggaaat
    22801 gatgcaactg ttacagttaa agtaggtgat caggtaacgc agggccagca attagttcaa
    22861 tataatacaa caacagctca gtcagcttat gatactgctg ttaggagtct taacaagatt
    22921 ggccgacaaa ttaatcatct taaaacatac ggagttcctg ctgttagtac agaaactaat
    22981 aaagatgaag ctaccggtga agagacgaca acaacagttc aaccatcagc tcagcaaaat
    23041 gctaattata aacagcagct gcaagattta aatgatgctt atgcagacgc acaagcagaa
    23101 gtaaataaag cgcagatagc gttaaatgat acagtagtta tcagtagtgt ctctggaact
    23161 gttgtggaag taaataatga tattgatcct tcttcaaaga acagtcaaac acttgttcac
    23221 gtagcaaccg aaggacagct tcaggtgaaa ggaacattga cagagtatga tttagcaaac
    23281 gttaaggttg gtcaatctgt aaaaattaag tctaaagttt attctaatca agaatggact
    23341 ggaaaaatat catatgtttc aaactatcca actgagtcta atgcaggttc aacaacgcca
    23401 gcaggtagca ctggagcggg aagttctaca ggagctgcct atgattacaa gattgatatt
    23461 ataagtcctc tcaaccagct taaacaaggt ttcactgttt ctgttgaggt tgttaatgaa
    23521 gctaaacagg ccttagttcc tttaacggct gttattaaga aagataaaaa acactatgtt
    23581 tggacttatg atgatgctac tggcaaagcc aaaaaagtag aggtgacact tggaaacgca
    23641 gatgcacaac aacaagaaat tcataaagga gtagctgttg gtgacattgt tattgccaat
    23701 ccagataaaa atatcaaacc ggataaaaaa ctagaagggg ttatttcaat aggtaccaac
    23761 acaaaaccgg aaaaagattc tcaatcaaag aataaaaaat caggggtgga taaatagtgt
    23821 cgcatgaaaa aaaacaatta atgcaattat caaatattgt gaagagctat caaaatgggg
    23881 atcaagttct taaagttttg aaaggtatta atttaacagt atatgagggt gaatttttag
    23941 ctattatggg tccgtctggc tcgggaaaat caacactaat gaatattatt ggtttattag
    24001 atcggccaac atccggagat tacacattac ataatactaa aatagaaatc cttaatgatc
    24061 gtgaattagc aaaagtacga aacgatgaaa tcggctttgt ctttcaacaa ttctttctgt
    24121 tagctaaatt aactgcatta caaaatgttg agttgcctct tatttatgct ggtgtaaacg
    24181 tctcaaaaag acgtgaacag gctaagcaat ttcttgaaaa agtaggactt ggacgacgta
    24241 tcaaacatct accgtcagaa ctgtcaggag gtcaaaagca aagggtggct atcgcacgag
    24301 ctttagtaaa tgacccatct attattttag cggatgaacc aacaggtgca ctagatacta
    24361 aaacaggtca gcaaattatg gaactcttga cagagttaaa caaagaaggt aaaacaatta
    24421 ttatggttac tcatgaacca gaaattgctg attttgcaac tcggaaaatt attattcgtg
    24481 atggtgatat cacaacggat actacagcta gtgttgttat tgactaggag gaaaaataat
    24541 ggaaaattgg aaattcgctc ttagctcaat ttgggggcat aagatgcgct caatcctgac
    24601 aatgttggga attatcatcg gtgtagcagc agttgtgatt attatggggt tgggaaatgc
    24661 tatgaaaaat agtgtaacaa gcacattttc tagcaaacaa aaagatattc aactctattt
    24721 tcaagaaaaa ggagaagaag aagacctgta tgccggattg cacacgcatg aaaataatca
    24781 cgaagtgaag ccagagtggt tagaacaaat tgttaaagat attgatggta ttgattctta
    24841 ttactttacc aattctgcta cttctactat ttcgtatgaa aaaaagaaag tagacaatgc
//
LOCUS       DAUGHY010000025        23426 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.025, whole genome shotgun sequence.
ACCESSION   DAUGHY010000025 DAUGHY010000000
VERSION     DAUGHY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23426
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.025"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            167..415
                     /locus_tag="VH752_001012"
     CDS             167..415
                     /locus_tag="VH752_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ8289374.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLNKTNN"
     gene            452..1063
                     /gene="amaP"
                     /locus_tag="VH752_001013"
     CDS             452..1063
                     /gene="amaP"
                     /locus_tag="VH752_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEQ8289375.1"
                     /translation="MLSRKEVCMSKLLKISYCLVGLVLLSVFGWVVGITGGYIYLPYS
                     YRWLSWGMDSFPNLLDSALSYYYFWTALVLFVITFLALLVIILYPRIYTEVQLRHKNK
                     KGTLLLKKSAIESYVATAIQTAGLMPNPTVTAKLYKRKFKIIVKGRLASRVAVADQIS
                     GVKEGIEKGITEFFGINYPVNFKVYVKDIADSDRKHITRNRVE"
     gene            1074..1262
                     /locus_tag="VH752_001014"
     CDS             1074..1262
                     /locus_tag="VH752_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEQ8289376.1"
                     /translation="MEFYEKFKYPIIGGLVGLIIAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            1275..1814
                     /locus_tag="VH752_001015"
     CDS             1275..1814
                     /locus_tag="VH752_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ8289377.1"
                     /translation="MTETYIKNTSKDLTSAIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            1855..2055
                     /locus_tag="VH752_001016"
     CDS             1855..2055
                     /locus_tag="VH752_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ8289378.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGFGKISGDKSLETEGKVDKVTGKIK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            2066..2554
                     /locus_tag="VH752_001017"
     CDS             2066..2554
                     /locus_tag="VH752_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ8289379.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTSAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            2717..3346
                     /locus_tag="VH752_001018"
     CDS             2717..3346
                     /locus_tag="VH752_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8289380.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVNDFLMQSQSVSKQYPLAPYEIICSQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNL"
     gene            complement(3501..4043)
                     /locus_tag="VH752_001019"
     CDS             complement(3501..4043)
                     /locus_tag="VH752_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ8289381.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAKIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKAVTLDN"
     gene            4161..4862
                     /locus_tag="VH752_001020"
     CDS             4161..4862
                     /locus_tag="VH752_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289382.1"
                     /translation="MAFIELKQVSKSYQVGETTVFANHEVSFEINKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHYTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMARTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            4877..7513
                     /locus_tag="VH752_001021"
     CDS             4877..7513
                     /locus_tag="VH752_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEQ8289383.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMALGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWPKRETDLVLSSSLAKNYQIGDELAVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSNLRLTNAFS
                     KDYQKRVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRAALAKETVKLAASEENL
                     TFLEGSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSALISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPITGYQKVSYASLTLPVKGLPDKQSISILSSSATSLSPYFNLLDS
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLVLTDRKGQSYKVTIKQVIDMTVGHYLI
                     MSNTYFKNHFKGLEAAPAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNANHIKSVQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNRHLKKVDMLEALKSID"
     gene            7605..8201
                     /locus_tag="VH752_001022"
     CDS             7605..8201
                     /locus_tag="VH752_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ8289384.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLINL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDSPIGQALLDAVDHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            8212..9168
                     /locus_tag="VH752_001023"
     CDS             8212..9168
                     /locus_tag="VH752_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ8289385.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGPQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVNTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            9265..10605
                     /locus_tag="VH752_001024"
     CDS             9265..10605
                     /locus_tag="VH752_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEQ8289386.1"
                     /translation="MNEIKCPHCHTLFTINESEYSQLLEQVRGQAFDEELQKRLINEI
                     ALLEEKAKHQLHEVVAKKETAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVISLTNQLDKLALEKDATFHSKLATIEKERDGIKNQL
                     VLQAKESELSLASVRSDYEAQLKAANEQVEFYKNFKAQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFVKDNQLSSRGSKGDYIYREVDTNGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKDCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAALNSLHYKQELALIKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVKRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFEALKDQ"
     gene            10721..11605
                     /gene="truB"
                     /locus_tag="VH752_001025"
     CDS             10721..11605
                     /gene="truB"
                     /locus_tag="VH752_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEQ8289387.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARAGESVERPRREVTISLFERTSPLNFT
                     EDGLCRFSFKVACSKGTYVRTLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKKVLI"
     gene            11634..12566
                     /locus_tag="VH752_001026"
     CDS             11634..12566
                     /locus_tag="VH752_001026"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEQ8289388.1"
                     /translation="MEIEYIKDYRDINQEDDTVLILGYFDGLHRGHKALFDKAREVAN
                     KEGLKVVVFTFTESPKLAFSRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFS
                     KVSSDHFITHYIKNLKAKHIVVGFDYKFGHNRTDSDYLTRNFEGQVYTIEEIKEDHRK
                     ISATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLP
                     ADGVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDK
                     IRDMEKFEGIEDLTDRLEYDKRTALNWKKDSKLS"
     gene            12609..13013
                     /locus_tag="VH752_001027"
     CDS             12609..13013
                     /locus_tag="VH752_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEQ8289389.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDVDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            13015..13293
                     /locus_tag="VH752_001028"
     CDS             13015..13293
                     /locus_tag="VH752_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEQ8289390.1"
                     /translation="MSGNYYYPLDLSWSTEEISSVLHFLNKVELAYEKKVDAKQLLDS
                     YKTYKTIVKSKAQEKQIDRDFQKVSGYSTYQVVKKAKAIEKGFFSLGN"
     gene            13283..14071
                     /locus_tag="VH752_001029"
     CDS             13283..14071
                     /locus_tag="VH752_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEQ8289391.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAVTLEDQ"
     gene            14074..15384
                     /locus_tag="VH752_001030"
     CDS             14074..15384
                     /locus_tag="VH752_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEQ8289392.1"
                     /translation="MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQL
                     KTFEHPIPNTLWGYYGKLSGKSQEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAA
                     PGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
                     HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALTMLKPGGELI
                     YSTCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQ
                     FVAKLKDKRQEGQSTKLKAPKSNLTKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLL
                     PDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVS
                     GNVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
     gene            15523..16389
                     /locus_tag="VH752_001031"
     CDS             15523..16389
                     /locus_tag="VH752_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEQ8289393.1"
                     /translation="MKMKKKFFLLSLLVLSTFFLSACSSWIDKGESITAVGSTALQPL
                     VEAVADEFGSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGS
                     RATFDSVIMKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFK
                     ANAKNVATNDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDME
                     VVKSHDGKVTKR"
     gene            16400..17335
                     /gene="pstC"
                     /locus_tag="VH752_001032"
     CDS             16400..17335
                     /gene="pstC"
                     /locus_tag="VH752_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEQ8289394.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFVDKVNLFDFLLGKEWQPSVKNAAGIPYLGALPMITGSFLVTILSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRSIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATRWQTIWRVVLNAARP
                     GIFTAVIFGMARAFGEALAIQMVVGNSAVMPSSLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            17325..18212
                     /gene="pstA"
                     /locus_tag="VH752_001033"
     CDS             17325..18212
                     /gene="pstA"
                     /locus_tag="VH752_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEQ8289395.1"
                     /translation="MNAKKVDKVATGTLYTIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSARFIGKKLHSKMTAAK"
     gene            18228..19031
                     /gene="pstB"
                     /locus_tag="VH752_001034"
     CDS             18228..19031
                     /gene="pstB"
                     /locus_tag="VH752_001034"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289396.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            19044..19802
                     /gene="pstB"
                     /locus_tag="VH752_001035"
     CDS             19044..19802
                     /gene="pstB"
                     /locus_tag="VH752_001035"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289397.1"
                     /translation="MTESILQIRDLSVYYNQKKTLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            19870..20523
                     /gene="phoU"
                     /locus_tag="VH752_001036"
     CDS             19870..20523
                     /gene="phoU"
                     /locus_tag="VH752_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEQ8289398.1"
                     /translation="MLRTKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWIVYLKTGKIIEL"
     gene            20728..23265
                     /locus_tag="VH752_001037"
     CDS             20728..23265
                     /locus_tag="VH752_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEQ8289399.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGLWTFDTTPRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIGNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNHHGFE
                     KKADESDEDEMVRQVALGRLWLAENQTIIDGLRTIFEAYQSNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQSTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
BASE COUNT         7398 a         3945 c         4841 g         7242 t
ORIGIN      
        1 attttgaaat atcggtattc aaacaaaaaa taaactaaca ctttattatt tagtttatat
       61 agtcactttt tgaaagtcga ctattaaaag actattgctt gaagaataac agctgttatt
      121 cttccttgga caaaaggtac aggagtatct aaaaaggaga attatcatgg gacttatttg
      181 gactttaatc gtcggtgcgc ttattggtgt gattgctgga gcacttacta aaaaaggtgg
      241 ttcaatgggc tggattgcaa acatcgctgc tggtctagtt ggttcttccg ttggtcaagc
      301 tttacttggt tcatggggcc cttctttagc aggaatgtct ttgattccgt cagttattgg
      361 tgccgttatc gttgttatga tcacttcttt tgtactaaac aaaactaaca actagtttcc
      421 tctttattta gtaagtactt acaaatagag aatgttaagt agaaaagagg tctgtatgtc
      481 aaaattgtta aagattagtt attgtcttgt cggacttgtt ctattgtctg tttttggatg
      541 ggtagttgga ataaccggag gctatatata tttaccttat agttatcgtt ggctaagttg
      601 gggtatggat agttttccca atctcttaga ttcagcttta tcctattatt acttctggac
      661 agcccttgta ttatttgtta taacatttct tgccttatta gttattattt tatacccaag
      721 aatttatact gaggtccagt tacgccataa aaataaaaaa ggaacacttt tattaaaaaa
      781 atcggctatt gaaagttatg ttgcgaccgc tattcaaact gcaggtttga tgccaaaccc
      841 aacagtcaca gctaaattat ataagcggaa gtttaagatt atcgttaaag gacgtttagc
      901 ttctcgtgta gcagtggcag atcaaattag tggcgtgaaa gaagggatcg aaaaaggtat
      961 aaccgagttc ttcggaatta attatcctgt taatttcaaa gtttatgtta aagatattgc
     1021 tgattctgat cgtaaacata ttaccagaaa tcgtgtagaa taggagatct atcatggaat
     1081 tttatgaaaa attcaagtac cctattattg gtggcttggt ggggctaatt attgccatat
     1141 tgctcatggc ttttggttta ttcaaaacgc tactagcaat tattttcatt attttaggaa
     1201 tatatggtgg cctgtatgcc aaaaaaacag gtattattga tcaatttctt aatcgtaaat
     1261 aaaggagact tattatgact gaaacttata tcaaaaacac atctaaagac ttaacttcag
     1321 caattcgtgg ccaattaaca tatgatgata aagtaattga aaaaattgtt ggtttagcac
     1381 ttgaaaatgt tgatgggtta ctgggtgtaa atggtggttt ttttgctaac ctaaaagata
     1441 aattagtaaa caccgaatct gtacgtgatg gcgtgaacgt ggaagttgga aaaaaacaag
     1501 ttgccgttga tcttgatatt gtcgctgaat atcaaaagca tgtgccgact atttatgact
     1561 ctattaaatc gatcgttgaa gaagaagtga agagaatgac cgatctggat gtgattgaag
     1621 ttaatgttaa agtagtcgac attaaaacaa aagaacaatt tgaagctgaa aaagtaagtt
     1681 tacaagacaa agtttcagac atggcacgtt caacatctga atttacgagt catcaagttg
     1741 agaatgttaa agcttcagtg gataacggtg ttgaaaaact tcaagatcaa aaagctgagc
     1801 cacgtgtaaa ataatcatat gtcgtcaatt aggtaagcta aaggagggtt tattgtgtca
     1861 gatgaaaaat ataatgcgaa attagatcaa gctggtggca agttaaaaga aggatttggt
     1921 aagatttctg gagataaatc tttagaaact gaaggtaaag tagataaagt tactggaaaa
     1981 attaaagagg ttattgcaga tgccaaagat actgtaaaag gattagctaa aggcttagat
     2041 aataaagata aataaagggg acatgatgga aaaaactcaa attaaaagca ctcttactta
     2101 tgacgataag gtcatcgaaa aaattgtcgg tcatgctttg gaaaacgttg gcggattact
     2161 agctgtaaca ggtggatttt tctcaaatat caaaaataat ttggtgaatt ctgagtctgt
     2221 aactgatggt gtttcagttg aagttggtag caaggaagta gcagttgatt tagcaatcat
     2281 tgtagaatac ggtaaagata ttcctgctat tgcagaatct attaaagcta ttgtttctca
     2341 aaacgttgac agtatgacac atttgaaagt tgttgaagtc aatgtgaacg ttgttgatat
     2401 tcgtactaag gaagaacatg aagctgctag cgttactgtt caagatcgtg tcactagtgc
     2461 agcttcaagt acttcacaat ttgtatcaga acaaacagaa aaattaaaag atactatctc
     2521 tgatactgta aatagtgatg aggcagctaa ataagcaatc gtctaggaag tattattgac
     2581 tagtggtgat aagtttgagc tggtatttac cagctctttt tgtcacggtt aaaaagatac
     2641 ggactattgt catctctcta aaaatatgat ttaattagat ataaattttt caaattgtta
     2701 gaaaggagta aattaagtga taagtcctaa aaaagagata acaagttcaa aatatcaaaa
     2761 aattgctatt tcagtagctc agcgtatcgc aaatggcgaa tatgaagtgg gcgaaaagtt
     2821 aaagtcaaga actactatcg cctcgacatt taatgtatca cccgaaacgg ctcgtaaagg
     2881 cctgaatatt ttagctgact tgaaaatttt gactttaaaa catggtagtg gagctattgt
     2941 gctgtcaaaa gaaagagcta ttgagtttat aaatcaatac gaatcaaccc attccatcgc
     3001 tgttttaaaa gaaaaaatac gtgagactat taatgatcag ggaaaagcaa tggaaaaaat
     3061 ggctgttttg gtcaatgatt ttttaatgca aagtcaatct gttagtaagc aatacccatt
     3121 agcaccttat gagattattt gtagtcaaga ttcggaacat tttggcaaat ctatcggtgt
     3181 gttaaatatc tggcaccaga caggagcaac tatcgtcgct attgaacatg ctggtcagtt
     3241 tatcgtttca ccaggtcctt attctgttat tgaaaaaggc gatcatattt atttcgttgg
     3301 tgatgagtca gtcatttcac gaatgaagac cttttttaat ttatgaaaag gattataagt
     3361 ttgagggccc acctcaaact ttttttgttg ctgttgagca ttattaaaag aggcagatca
     3421 tcatggtttg tctttccaaa atatctgttt agacagttgt tttggattgg gagcatgatt
     3481 tcagactctt ttgatgtttt ttaattgtct agagtaactg ctttgagaat aatatcggca
     3541 acctcttcag tggtttcttg atagccatta gcaatccaat agctgataat gctctcaata
     3601 ctggcaaggt aaacttctaa agcataggga taaggaatat ggtattgatt ggtcacaatg
     3661 tcttggaaac cagtaatagt agtcaaaaat tggtaacaga aatccttgat gagttgcgga
     3721 aatttgaggt aagaaatagt agcgagtgcg gttataaatt ctctatgttc tataaggtag
     3781 gacaaggttt gattgaggac ttgtcgagtg tctgtgtaga ttttagcctg atttaacaaa
     3841 cggtacaaat catccagagt gtcatttttg aagtgattca tcatgtcaaa tttatcagtg
     3901 tagtggaggt agaaggttcc tctattgata ccagcctttt tagtaaggtc agagacagta
     3961 agagtttcaa aagattgttc agttagtaag gtggttaagg cggtctttac ataagctttg
     4021 gtttcagtat gtcttttggt cattctcagg tgctccttaa atcaacagtt gcgtcagatt
     4081 tgttcttgtt aaatacttag ggccattata taataaggct ataattaaat caacactatg
     4141 ttcaaataat ggaggggcag atggctttta ttgaattaaa acaggtttca aaatcttatc
     4201 aggtaggaga gacgactgtt tttgccaacc acgaagtgtc ttttgagatt aataaaggag
     4261 aactagtggt tattttggga gcatccggag ctggtaaatc gacagtcctc aatattttgg
     4321 ggggcatgga cacagtagat gcaggtcaag taattattga tggcaaagac attgctcatt
     4381 acacgtctaa ggccttaact cagtatcgtc ggaatgctat tggtttcgtt tttcaatttt
     4441 ataatttggt tcctaatttg acagctaaag aaaatgttga attagcagtt gaaattgtag
     4501 cagatgcttt agatcctgtg accattttaa aggaagtagg actcagtcat cgtctggatc
     4561 attttcctgc tcagctctca ggtggtgaac agcaacgggt ttcgatagca cgtgccttag
     4621 ctaaaaaccc taaattgctt ctttgtgatg aacctacagg tgcccttgac taccaaacag
     4681 gaaagcaaat tctaaccctc ttacaggata tggcacgaac taaggggacc acggtagtta
     4741 ttgtaaccca caatgctgct attgctccga tagctgatcg tgtcattttt atgcatgatg
     4801 cacaggttac caagacagtc attaataagg aacctgcaag cattgagaca attgattatt
     4861 aagaggaagg tgtcgtatga taaagaaaac cttatggaag gatattttga gagctatcaa
     4921 gaatagtaaa gggcgattca tttccttatt tttcctaatg gccttaggct cctttgcttt
     4981 agttggactt aaagtcaccg gtccagatat ggaacgaaca gccagccgtt acctcgaaag
     5041 acaccaggtg atggacctaa cggtactggc ttctcatcaa ttttcccaag ccgataaaca
     5101 agagttagat acgttaaaag gggctcattt agaatatggt catttacttg atgtcagtct
     5161 aactagcaac cagaaatctc ttaggctcta tagcgtgcca aagaaagtgt ctaagccagt
     5221 cttggttaaa gggagttggc caaagcgaga gacagatttg gttttatcct cctcacttgc
     5281 taaaaactat cagattggag atgaactagc agtcacctca cctatggaag gtttgctgac
     5341 gacaacgcat tttcaagtgg ttggttttgc gaattcttca gaagtttggt ccaagtccaa
     5401 tttaggaagt tcctcaaccg gagatggaag tctctacgct tatgcttttg ttaatccaaa
     5461 cgtttttaaa agtgccttta acctgctgcg catccgtttt agtaatcttc gtctgacaaa
     5521 cgctttttcc aaagactatc aaaaaagggt aacccaaaac caagctcatt tggataatct
     5581 acttaaagat aatggccaga agcgttatga tgacctccaa aatcagtatg accttgcctt
     5641 aaaaaatggc agagcagcac ttgcaaagga aacagtgaaa ctagctgcga gtgaggagaa
     5701 cttaactttt ttagaaggct ctgctttaca agaagctaag catcagattg aacaaggcaa
     5761 acaagcatta gccaaggagg aaaagcagtt agagcaggtg caggctacaa aagataagct
     5821 agaaaaaccc agttacctga cttataatcg ctcgacccta ccaggaggag aaggatatca
     5881 tacttatgca acttcaacga cctccatttc aaatgttgga aatatttttc ctgttgttct
     5941 ttatctcgta gctgctctag tagcgtttac caccatgaca cgttatgttg atgaagaaag
     6001 aacaagctct ggtctattaa aagccattgg ttattctaac aaggatatca gtttaaagtt
     6061 tcttatctat gggcttttag ccagtttttt agggacaact ttaggtatta ttgggggaac
     6121 ttacctctta tctgccttga tttcagagat cttaacagga gctttgacta ttggaaagac
     6181 tcacctttat agttattggt tttataatgg catagcttac ttgctggcta tgttatctgc
     6241 tgttttacca gcctacttaa ttgtcaaaaa ggaattattc ctcaatgcag ctcagttatt
     6301 gctgcccaaa cctcctagta agggggcaaa aatctggttg gaacacctta cttttgtctg
     6361 gaaagccctg tcctttactc acaaggtgac catacgtaat atttttcgct ataaacaaag
     6421 aatgctgatg accattgtag gcgttgcggg ctcagtagcc cttttatttg caggcttagg
     6481 gattcagtcg tcattagcca aagtagttga gcatcaattt ggtgatttaa cgacttatga
     6541 tattttggct gtcggttcgg ccaaagcgac agcgacagag caaaccgact tagctagcta
     6601 tcttaaacaa gaacctatta cagggtacca aaaggtatct tatgccagct taacccttcc
     6661 tgtaaaggga ttacctgata agcaaagtat ttccatttta tcaagttcag ctacttctct
     6721 tagcccctat tttaatctgc tggatagtca ggagcaaaag aaggttccca ttccaacctc
     6781 tggtgttttg atttctgaga aattagcctc ctattacaag gtaaaaccag gtgatcagtt
     6841 ggtattgact gatcggaaag gacagtctta taaagtgacg attaaacagg ttattgacat
     6901 gacagttggc cattacctga taatgtctaa tacctatttt aagaatcatt ttaaaggatt
     6961 ggaggctgct cctgcctatc tgattaaggt aaaagacaaa gatagcaagc acataaagga
     7021 gacagccagt gacttgttaa ccttaaaagc gattagagca gtttcacaaa acgccaatca
     7081 tattaaatct gttcagctag tagtcacctc tcttaatcag gtcatgaccc tccttgtctt
     7141 cttgtctatt ttattagcaa tcgttatcct ttataactta acgactatta atattgctga
     7201 gcgtattcga gaattatcca ctattaaagt tctgggattt tacgatcagg aggtcacctt
     7261 atatatttat cgagaaacta tttcgctatc cctagtaggc attcttttag gtatctattt
     7321 aggaaaaggc ctgcatactt atatcatgac aatgatttca actggggata ttcaatttgg
     7381 tgtaaaggtt gatgcttatg tttatctagt gccaattcta gtaatcctta gcttgttagc
     7441 ggtattaggt atctgggtta atcgccattt aaaaaaggtt gatatgttag aagctttgaa
     7501 atccatagat tgattggtga aaagacagag atagacagca cttgaaaagt taatcatatt
     7561 atgatagtat ctaaatacta tgatttaata gaaagatact atgtatggaa aatagcattg
     7621 aggcctatct agcagatagc agagaaaagg taacattagg tacagttgtt gattgtttta
     7681 aaggaaaagc agtctcaagt aaggttgtgc caggtgatgt gggattgatt aatttatctg
     7741 acatgggcac cttaggaatt caatatcacc aattaagaac tttccagatg gatcgtaggc
     7801 agctcttgcg ttaccttttg gaagacggag atgtcttaat cgcctctaag ggaaccctaa
     7861 aaaaagtctg tgtttttcat aagcaaaatc gagatgtggt ggcatcatca aatatcactg
     7921 tcttacgccc tcaaaaactg ttaaggggct actatatcaa gttctttttg gactcaccta
     7981 ttggtcaagc gctcttggac gcagtggacc atggcaaaga tgtgatcaat ctctcaacga
     8041 aagaattgtt ggatattcca attcccgtta ttcctttagt gaaacaggac tatttgatta
     8101 atcactactt gagaggatta acagattatc atcgtaaact caatcgagca gaacaggagt
     8161 gggaatatat tcagaatgaa attcaaaaag gattaggtta ggagtcttgt catgggttca
     8221 gttgaacgta cattaaaaat gaccttggct accattgttg ccattttaat tgcttaccaa
     8281 cttcatctgg attatgcaat gtcagcgggg atcattgccc tgttaagtgt tttagacacc
     8341 cgtaagtcca gtttggtggt cgctaggaat cgcttgctat cttttttcct ggcttttggg
     8401 attgcgatga tgtgctttag cttgtttggt tttacgacag taggttttat gtgttatttg
     8461 ttaattatta ttcctttgct ctatcacttt cagatagagg caggactggt tcctataaca
     8521 gtacttgtga cacatttgat tgctaaaaag agtattgcat tacctatttt atcgaatgag
     8581 tttatgctct tttttgtagg gactagtgtc gctttactat ttaatgctta tatgggtcca
     8641 caagatcagc aaattcggta ctatcaccaa aaagttgaat cggatctgaa aggtatctta
     8701 taccgttttg aaagttttct gctggaagga aaaggtcaaa atgaagggct cttgatcaaa
     8761 aaccttgata aaatattaga tgaagctcta aaactagttt atcgagaaag gcataatcaa
     8821 ctatttcagc agaccaatta tcaagttcat tattttgaga tgcgacgaca acaaaataga
     8881 ctattgggac aaatggctat caacgtaaac acgttaatga gacaaagtaa ggaaagtatt
     8941 cttttgtcac atctttttca cgaaactgct tgtcagctaa gcgaacaaaa tccggcttta
     9001 accttgattg atgacatcga acaattgctt gaaacctttc gtcatggtga tcttcctcaa
     9061 actcgggagg aatttgagcg acgtgcggtt ttatttcagc tcttacaaga cttggaacgt
     9121 tttatcttat taaaggtaga gttttatcag gattatcaaa atgactaaat ctctattgca
     9181 cacttcttat ctatgacagc ttgggactta acttcctgag cttttttgct ataataaaaa
     9241 caagaatctc agaatggaga caagatgaac gaaataaaat gccctcattg tcatacccta
     9301 tttactatca atgagtcaga gtatagtcaa ttattggaac aagtcagagg gcaagccttt
     9361 gatgaagaat tgcaaaaacg gctaatcaat gagatagcct tgctagaaga aaaagcgaag
     9421 catcaattgc atgaggttgt cgcaaaaaaa gaaacagcta ttaccagctt gacaaaccaa
     9481 ttagagcaaa ttgaaaaaga acaagcctac ctcagacaag aagaactggc taaaaaagac
     9541 cagcttattg cttctttaga agcgaaactt gataaattag cctcacaaaa tgctttggaa
     9601 ctggccaatc aattagctga gaaggacaaa gaagtgatta gtttgaccaa tcagctggac
     9661 aaattagcac ttgaaaaaga tgctacgttc cactcaaagc ttgctacaat cgaaaaagaa
     9721 cgcgatggaa tcaaaaacca attggtgctt caagctaaag aaagtgaatt gtcgttggct
     9781 tcggtgcgca gcgactacga agcacagtta aaagcggcta acgagcaagt tgaattttat
     9841 aaaaatttca aagcgcagca atctaccaaa gcaatcggag agagtctaga actctatgct
     9901 gaaacagagt tcaataaagt aagaagctat gcttttccca atgctagctt tgtcaaagat
     9961 aaccaattgt ctagtcgtgg gtcaaaaggg gactatattt accgagaggt tgataccaat
    10021 ggtgtagaaa tcctctctat catgtttgag atgaagaacg aggcagacac caccaaaaca
    10081 aaacataaaa atagtgattt tttcaaggag ttagataaag accgtcgcga aaaagattgt
    10141 gagtatgctg ttttggtaag catgttagaa gctgataatg attactacaa tacagggatt
    10201 gttgatgtca gtcatgagta tcaaaagatg tatgtggttc gtcctcaact ctttattcag
    10261 ttgataggga ttctgcgaaa tgcagccctg aatagtttac attataagca agagttggcc
    10321 ttgattaagg aacaaaatat tgacatcact cacttcgaag aggatttgga ccaatttaag
    10381 aatgcctttg ctaagaacta tcagtctgcc agcaacaact ttaaaaaggc tattgacgag
    10441 attgataaat cgattaaacg catggaggaa gtgaaacgct ttttaaccac tagtgaaaat
    10501 cagttacgct tagctaataa taaattagag gatgtttcag ttaaaaaact gacccgccaa
    10561 aacccaacca tgcgagagaa atttgaagcc ctcaaggacc agtaagcctg aaagtgagtc
    10621 tttccctgct aaaattggtt aagcgtttat tttggcaaaa ctgatgataa agcggtagaa
    10681 tagaatcagt aataattact aattagagag aaaaatagac atgataaatg gaatcattaa
    10741 tttaaaaaaa gaagccggca tgacctctca tgacgcagtt tttaaattac ggaagttatt
    10801 acaagaaaaa aagattggac atggggggac cttagatcct gatgtggtcg gtgttctacc
    10861 aattgcagtt ggtaaggcta ctcgtgtgat cgaatacatg acagaagctg gtaaagttta
    10921 tgaaggacaa gtgactctcg gttactcaac aacgactgag gatgctagtg gagaggtagt
    10981 tgcgaggtcc tctttgccag ctgtgctaac agaagaactc gttgatcaaa cgatgacgac
    11041 tttccttggg aaaatcacac agaccccacc tatgtattca gccgtaaagg ttaatggccg
    11101 taaattatac gagtatgcgc gtgctggtga aagtgtggaa cgtcctagaa gagaagtgac
    11161 tatttctctg tttgaacgca ccagcccttt gaattttact gaagacggtc tttgccgatt
    11221 ctcttttaaa gttgcttgta gcaaagggac ttatgtgcgg accttagcag ttgatttagg
    11281 tcgagctcta ggggtagaga gtcatatgtc cttcttgcag cgatcagctt cagcaggatt
    11341 aaccctcgag actgcctata ctttgggaga aattgctgat atggtttcta aacaagagat
    11401 gtcttttttg ttaccgatcg agtatggcgt tgctgatctt cctaaaatgg ttattgatga
    11461 tactgaattg actgaaattt catttggtcg ccgtcttagt ctccctagtc aagagccact
    11521 gttagctgct tttcatggcg aaaaagtaat tgctatttta gaaaaaagag atcaagaata
    11581 caaacccaaa aaagttctta tttaaatagt ttgagtaagt agaatggata attatggaaa
    11641 ttgaatatat caaagattat cgtgatatta accaagaaga tgatacagtc ttgatattgg
    11701 gatattttga tggattacac cgtggtcata aagccttatt tgacaaagca agagaggttg
    11761 caaataaaga agggttaaaa gtagtcgttt ttacgtttac agagtctccg aaattggctt
    11821 tttcgcgttt ttctcctgag ttattgcttc atattactta tccaaagaag cgttatgaaa
    11881 aatttgccga ttacggtgta aataaattat atttggttga ttttacttct aaattctcta
    11941 aggtctcatc tgatcatttt ataactcatt atattaaaaa cctgaaagca aagcatattg
    12001 ttgtcggttt tgactataag tttgggcata atcgtacaga tagtgattat ttaaccagaa
    12061 attttgaagg acaagtttat accattgagg aaataaaaga agatcatcga aaaatttctg
    12121 ctacatggat aaggaagctt attcaagaag gtaatgtagt taaagcaaac cacttgttag
    12181 gctatgactt atcgacaagg ggaagagtcg ttcacgggga tgcgcgtggc cgtactattg
    12241 gtttcccaac agccaatcta gctccaattg acaacacata cttaccagct gatggagtct
    12301 atgttacaaa tgttattgtt gctaataaaa tctatcgctc tatgacgagt ttaggtaaaa
    12361 atgtgacatt tggggggaaa gagttgcgtc tagaagttaa catttttgac tttgacgaag
    12421 agatctatgg agaaattatt gagattgttt ggttagataa gattcgagac atggaaaaat
    12481 tcgagggaat cgaagatttg actgatagat tggaatatga taaacgaaca gcattaaatt
    12541 ggaaaaaaga tagcaaatta tcttaaattt ttgtataata aggtaagtaa atttgataga
    12601 ggaaaaatat ggttacctta tttttatcac ctagctgtac cagttgtcgt aaagcaaggg
    12661 catggttagt aaaacacgaa gttgactttc aagagcataa tattattaca agtccattaa
    12721 gccgtgatga attaatgtca atcctctctt ttacagagaa tggaacggag gatattattt
    12781 caacacgttc aaaggttttt caaaaacttg atgttgatgt agaagagctt tcaatttcag
    12841 atttaattga tttaattgct aaaaatccaa gtcttcttcg tcgccctatt atcatggatc
    12901 aaaaacgcat gcaaattggt tttaatgaag atgaaattag agcgttttta tcgagagatt
    12961 atcgcaagca agaactacgt caagcaacga taaaagcaga aattgagggg tgatatgtca
    13021 ggtaattatt attatccact tgacttaagt tggagcactg aagaaatatc ctcagtgctt
    13081 cattttttga ataaagtgga gttagcatat gaaaaaaaag tagatgcaaa acaactccta
    13141 gatagttata agacttataa aaccattgtc aaaagtaaag ctcaggaaaa gcaaatcgac
    13201 cgtgattttc aaaaagttag tggttattcg acctatcaag ttgttaaaaa agcgaaggca
    13261 attgagaaag gattcttttc ccttggaaac taaatacgca tttgcaaggc aaatcattaa
    13321 agaagcaggg ctttttatca agtcaaaaat gtcagagcag ctagatattc aggtgaaaac
    13381 acaatttgat gatctggtta ccaatgttga tcaagaaact caacagttac tgatggatcg
    13441 tattcatcag acctatcctt gtgatgccat tttagctgaa gaaaatgacg ttcgtcaccc
    13501 cattaatcaa ggaaatgttt gggtcatcga tcccattgat ggaacggtta actttattgt
    13561 gcagggatca cagtttgcag tgatgattgc ctattatgaa caaggaattg gccaatttgg
    13621 gcttatttat gatgtgatgg cagatcaatt actagctggt ggtggtgatt ttgaagttac
    13681 tttaaacggt gataagcttc cagcttacca agaaaaacct ttagagcgta gcttaattgg
    13741 ctgcaatgca ggcatgtttg ccagaaatga tcgtaacctt gctcatctaa ttgcaaagac
    13801 tttaggtgta agggtttatg gtggtgcagg tatctgcatg gttaaggtca tgaagcagga
    13861 attactggcc tatttttcat ttatccaacc atgggattac gcagcagcaa aagtactagg
    13921 cgataaactg ggttatgttt tactaactat tgacggatat gagcctgatt ttcaaacacg
    13981 tcaaaaaatc atgtttgttc ctaaatgcca actcactagg atagcgtctt ttctaacaaa
    14041 ggactctgcc gtcacacttg aagatcaata gaaatgatgt cattaccaaa agaatttatt
    14101 aacacttatc aagcaatact aggcaaagaa ttagaagatt tcttggcaag ttttaaccaa
    14161 gagccggtca atgcttttcg gattaacccc ttaaagaatc aactaaaaac ctttgaacac
    14221 cccattccaa ataccttatg gggatattat ggtaaactat ctggcaagtc tcaagagcat
    14281 gtgtcggggt tggtttattc ccaagaacca gcggctcaaa tggtggcaca agtagcggct
    14341 cctcaaaagg ggagtcgtgt tttagacttg gcagctgcac caggaggaaa atcaactcat
    14401 ttattagctt atttagacaa tacaggtctt ttagtcagca atgaaatttc taagaagcgc
    14461 agcaaggttt tagttgaaaa tatcgaacgt tttggcgcta gaaatgttgt ggtgactaat
    14521 gaatccgcag atcgtttagc caaggtattt tctcattatt ttgatactat cgtctttgat
    14581 ggaccatgtt ctggtgaagg aatgtttcgc aaggaccctg atgctattca atattggcat
    14641 cacgggtatc ccgcagaatg cgccaagttg caaaaatcta ttttagaaga cgccttaacc
    14701 atgttaaaac caggaggaga attgatatac tccacctgta cttgggctcc tgaagaaaat
    14761 gaagatgttg tgcagtggct cttagagact tatacctttt tagaattggt cgatgttcca
    14821 aaactaaatg gaatggttag tggtatcgga cttccagaga ctgctagaat gtaccctcat
    14881 cgttatcaag gggaagggca atttgtggcc aagctaaaag ataagcgtca agaaggacaa
    14941 agcactaaac taaaagcacc taaatccaat ttgaccaaag accagttacg tctttggaaa
    15001 atgtttgaaa aagaccatct aaaccttacc ctatcaggaa cactacaaac ctttggtgat
    15061 tacctatacc tcttgccaga tggtcttcct gctttagatg gccttaaaat tgctcggaac
    15121 ggtttagaat taggcgtctt caagaagaaa cgctttgaac cctcttacgc cttgggatta
    15181 gctttaaagc ctgatgaggt ccgatcatct attgagctta gagaggaaga ctttagacct
    15241 tatgtgtcag gtaacgtcat tactttgccc caatcttatc caacaggctg gtaccagtta
    15301 ctcattaatg gcaatggtct aggatttgcc aaggtcgttg acaagaccat caaaaataat
    15361 ttccctaaag ggttaagatt ttagcgtaaa aaataagatt aaaaagttaa atatttaaag
    15421 aaaaaatgct ttcaaagcat aggtagatat gttactataa agaatatgtg gcacttagtt
    15481 tctacatata taccctttat ttttacgaat aggaaataaa taatgaaaat gaaaaaaaag
    15541 ttctttttgt taagtctttt ggtcctatca actttctttt tatccgcatg ttctagctgg
    15601 attgataaag gtgagtcaat aaccgctgta ggatcaacag cactacaacc cttagtagaa
    15661 gcagtagctg atgaatttgg aagcagtaat ctaggcaaga ctgtcaatgt tcaaggtggt
    15721 ggttcaggta cagggttgtc tcaagttcaa tcaggagctg tccaaattgg aaatagtgat
    15781 gtctttgcgg aagaaaaaga tggtattgat gcttctaaat tagttgatca tcaagtagct
    15841 gttgcaggac ttgcagttat tgccaatcct aaagtcaagg tttccaatct cagtagtcag
    15901 cagttgcaaa agattttttc aggagaatat accaattgga aacaagttgg aggagaagat
    15961 cttgcgattt cagtgatcaa ccgagcagca agttctggct cacgagcaac ctttgacagt
    16021 gttatcatga aaggggtcaa cgctaaacaa agtcaagagc aagactccaa tgggatggtt
    16081 aaatcgattg tttcacaaac accaggtgcc atttcttacc tttcctttgc ctacgttgat
    16141 tcatctgtta aatctttgca attaaatggg tttaaggcaa atgctaagaa cgtggctaca
    16201 aatgattggc caatctggtc ctacgaacac atgtatacca aagataaacc aacagggttg
    16261 accaaggaat ttcttgatta tatgttttca gatgaagtac aacagaacat tgttacacat
    16321 atgggatata tttcgataaa tgatatggaa gtggtcaaat ctcatgatgg aaaagtaaca
    16381 aaaaggtaat ggaggaatta tggaaaatca ggaattggct aaaaaattag cctcaccatc
    16441 aaagaattca aggcttgaaa cctttggccg caccattacg tttttgtgtc tggctttgat
    16501 tgtttttatc gttgcgatga ttctgatctt cgttgctcaa aaagggttgt caaccttctt
    16561 tgttgacaag gttaatcttt ttgatttctt gcttggaaag gagtggcaac caagtgtgaa
    16621 aaatgcggcc ggcattcctt atcttggtgc tctgccaatg attacaggat cctttttggt
    16681 taccatttta tctgctatta ttgccacccc atttgccatt ggcgcagccg tttttatgac
    16741 tgaaatctca cctaaatatg gcgctaaatt attacagcct gcggttgagc ttttggttgg
    16801 aattccttcg gttgtttatg ggtttatcgg tttgcaagtg attgttcctt ttatgcgctc
    16861 tatctttggt ggcacaggtt ttggaatcct atctggggtc tgtgtcttgt ttgttatgat
    16921 tttaccaaca gtgactttta tgacaacaga cagtctgcgg gcggtgcctc gtcattaccg
    16981 cgaagcgtct atggctatgg gagcaacacg ttggcaaacc atctggcgtg ttgtccttaa
    17041 tgcagctcgt ccaggaattt ttaccgctgt tatttttgga atggcgagag cttttggaga
    17101 agccttggct atccaaatgg tagtcggtaa ctctgctgta atgccaagtt cactaacgac
    17161 accagccgca accttaacgt ctgtcttgac aatgggtatt ggtaacacgg ttatgggaac
    17221 ggtccaaaat aatgtgcttt ggtcccttgc cctagtctta ttattaatga gtctggcctt
    17281 caattccctt gtcaaattaa tcacgaaaga aagaaagaga aattatgaac gctaaaaaag
    17341 tcgataaagt agcaactggt actctttata ccattgctgg aattattgta gctattttag
    17401 cttccttaat tctatatatc cttgtccgtg gcttgccaca catcagctgg tcctttttaa
    17461 caggaaaatc gtcttcgtac gaagctggtg ggggaattgg tatccagttg tataattcct
    17521 tcttcctatt gattgttact ttaattattt ccattccttt atcaactgga gcggggattt
    17581 acttggctga atatgccaaa aaagggcctg ttaccaactt tattagaacc tgtattgaga
    17641 ttctgtcttc cctaccatct gtggttgtgg ggctctttgg ttacttgatt ttcgttgtgc
    17701 agtttgaata tggcttttct attatttcag gggctcttgc tttgacggtc tttaatcttc
    17761 ctcaaatgac ccgtaatgtt gaagatagtt tactgcatgt tcatcatacc caaagagaag
    17821 caggattagc tttaggcctg tcacgctggg aaacggtttt ttatgtggtt atcccagaag
    17881 ctctcccggg aatggtaaca ggtattgtct tagcttcagg tcgtattttt ggtgaagcgg
    17941 cagcgcttat ctatactgct ggtcaatcag caccagctct ggattggtca aattggaatc
    18001 cacttagtgt tactagtcca atctctattt tccgtcaatc tgagaccctt gctgttcaca
    18061 tttggaaagt caatagcgaa gggacaattc cagatgctac tcttgtatcc gcaggaagtg
    18121 cagcagtgct attaattttt atccttattt tcaacttttc agctcgcttt attgggaaga
    18181 aacttcattc taaaatgaca gcggccaaat aaaatagtgg agaaatcatg acagaataca
    18241 attggaatga acgtcatatc atcacttttc ctgaagaaac gcttgctcta gctaccaagg
    18301 atttacatgt ttactatggt gcgaaagaag ctatcaaagg gattgatatg cagtttgaaa
    18361 aacataaaat aacagccttg attggtccat ctggttgtgg caaatcaact tatcttcgta
    18421 gtcttaaccg catgaacgat accattgata ttgctcgggt tacaggtgag attctttatc
    18481 agggcattga tgttaatcga aaagacatga atgtttacga gattcgcaag cacttaggaa
    18541 tggtttttca aagacctaat ccttttgcta aatccattta taaaaacatt acctttgccc
    18601 atgaaagagc tggtgtgaaa gacaaaaaag ttttagatga aattgttgag acttcactga
    18661 aacaagccgc gctttgggat caagttaaag atgaccttca caaatcagcc ttcaccttat
    18721 cagggggtca gcaacaacgc ttgtgtattg ctagagccat ttctgtcaaa cctgatattc
    18781 tattaatgga tgaaccagcc tctgcccttg acccgattgc aacgatgcag ctggaagaaa
    18841 ccatgtttga attgaagaag aattacacga ttattattgt gacccacaat atgcaacaag
    18901 cagctcgtgc cagtgattac actgccttct tctatttagg caacctcatt gaatatgata
    18961 agacacgtaa tattttccaa aatgcgcaat gtcagtcaac aaacgactac gtttcagggc
    19021 actttggtta gaaaggcaat atgatgacag aatctatttt acagatcaga gacctttctg
    19081 tttactacaa tcaaaagaaa acgttaaaag atgtctcttt ggacctttat ccaaatgaaa
    19141 taacagcttt aattggtcct tctggatctg ggaaatcaac gctcttgcgt tctatcaacc
    19201 gtatgaatga tttgaatcct gaagtaacca ttacaggctc tatcgtctat aacggacaca
    19261 atatttatag tccaaggaca gatacggttg atttacgcaa agaaattggt atggtattcc
    19321 aacaacccaa cccatttccg atgtctattt atgagaacgt ggtttacggc cttcgcttaa
    19381 aaggtatcag agataaatca atcttagacc atgctgttga aagctctctt aaaggggctt
    19441 ccatttggaa tgaagtcaaa gatcgtttac atgattctgc ggttggctta tcaggcggac
    19501 agcaacagag agtttgcata gctagagttc ttgctaccag ccccagaata atccttttag
    19561 atgaacctac ctcagctctt gatcctatct cagctgggaa aattgaagaa acattattgt
    19621 tattaaaaaa agattataca ctggctattg tgacccgctc aatgcagcaa gcctcacgtt
    19681 tatcagatcg aacaggtttt ttcttggagg gtgacttact tgagtgtggt cctacaaaag
    19741 ccatgtttat gaatcctaag cgcaaagaaa ccgaagacta tatttctggt aaatttggtt
    19801 gagaaagacg tatatggttg acccctctgt gtttttatta taatgactag attgtaggaa
    19861 gaaaaaatca tgttaagaac gaaatttgaa gaagaattag ataaattgca taaccagttt
    19921 tattcaatgg gaacggaagt attggcacaa atcaataaaa ctgttcgtgc ctttgttagt
    19981 catgatcgtg aattggccaa agaagttatc gaagaagatg acactattaa taattttgag
    20041 acaaagcttg aaaagaaatc gctggaaatt attgccctac aacagccagt ttcgaatgat
    20101 cttagaatgg ttattactgt tttaaaggca tcaagtgata ttgaacgtat gggtgaccat
    20161 gctgcttcga ttgccaaggc cactattcgt atgaaaggtg aggagcgtat tccagtggtt
    20221 gaagaacaaa tcaaccttat gggaaaagct gtgaaacaga tggtagaaga agcccttaat
    20281 gcatatatca atgctgacga tacaaaagct tatgaaatcg cagcttctga tgagattatc
    20341 gatcaatatt tccgtaatat ccaaacctta gctgttgaag aaatccgtaa gtctccagat
    20401 gctgtttttg caggaaaaga atattttcaa gtattaatgt atttggaacg tattggtgac
    20461 tatgctcgca acatttgtga atggattgtt tatcttaaaa ctggaaaaat tattgaatta
    20521 taagaaatta tgctataatg agactgggaa tcagcttgcc agtccctggt tttatacgaa
    20581 attgtgcaag ttagaaaatg aactaacaag aaaagttttc tttgctgaat ctgattttgt
    20641 taggttaaca aatatatgta gacagagatg atacttaggt atcatctgtt gatctattat
    20701 gatttgttat tagaattaag gagttatatg aagacagtag aacatctcat cgaaacattt
    20761 gtgcctgaaa attataacat ctttttagat attaatcgtc agactaaaac atttacgggg
    20821 aatgttgcga ttaatggtga agctttagat aatcatgtgg ctttccacca aaaagatttg
    20881 gacattaagt ctattttgct cgataatgaa gctgttattt atcaagtgga taacgacaat
    20941 gaagttgtgc gtgttgaatt acctgaaaca ggaatgatga cgcttgtcat tgaattttca
    21001 ggtagcatta cagataatat gacaggtatt tacccttcat attacactaa aaatggtgag
    21061 aaaaaagaag tcatttcaac tcagtttgaa agccattttg cgcgtgaagc tttcccatgt
    21121 atcgatgaac cacaagcaaa agctactttt gatttaagct tgacctttga tcaagaaatt
    21181 ggtgagatag cgttatcgaa tatgccagaa gtcaatattg accgtcgcga ggagactggt
    21241 ctttggacat ttgatacgac accaagaatg tcatcttact tgctagcgtt tgcgctagga
    21301 gaacttcatg gaaagactgt agaaagcaaa aaaggaacaa ctgttggtgt gtatgcgaca
    21361 acagcgcatc ctcttagctc gcttgatttt tcattggata ttgcagttcg tgttatcaac
    21421 ttttatgaag actattttgg agtacattac ccaatcccgc aatctcttaa tattgcgctt
    21481 ccagattttt catctggtgc tatggaaaat tggggtttaa tcacttatcg tgaaatttat
    21541 ttattagtag atgaaaattc gactgttcaa agccgacaac aagttgcact tgtaatcgcc
    21601 cacgagatag cacaccaatg gtttggaaac ttggttacaa tgaaatggtg ggacgatctg
    21661 tggttaaatg agagttttgc caacatgatg gaatatgtct ctatcgaggc tattgagccg
    21721 tcttggaaaa taatagaaga tttccaaaca ggtggtattc ctcttgcttt aaaacgtgat
    21781 gctactgatg gtgttcaatc agtgcatgtt gaagtcaatc atccagatga gataaataca
    21841 ttgtttgatc ctgctattgt ctatgcaaaa ggtagtcgtt tgatgcatat gttacgccgt
    21901 tttattggtg atcgtgattt tgccattggc ttacatcact actttgaaaa atatcaatat
    21961 cgaaatactg tcgggcgtga tttatggaat attttatcag atacttcagg caaagacgta
    22021 gctgctttta tggatgcttg gcttgaacaa ccaggttatc cagttttaac agctcgcctt
    22081 gaaaacgatc aattgatttt gagtcaaaaa caatttttta ttggaaaagg tgaagaaaaa
    22141 ggtcgccttt ggccgattcc tttaaacact aattggcatg gccttcctga aactttgaca
    22201 gaggcagaaa tggtcattcc aaactttagt caattggcag cagaaaatga aggagctcta
    22261 cgtttcaata ttggtaatac agctcattac attacagact accaaggatc attattagat
    22321 gccttagtca ctgaacttgc ccaacttgat aatacttctg ctttacaagt tattcaagaa
    22381 cgtcgtttat tggcagatag tggcttgatt tcttatgctg aattggttga cttaattgct
    22441 caattggacg attctaagtc ttatatggta gcagaagcgg ttcagcaagt ggtctctggt
    22501 ttgaaacgct tcattgatga aggaagctta gcagaaaaat cctttaatcg cttggtaacc
    22561 accatctatc aagaagactt taatcaccat ggttttgaga aaaaagctga cgaatcagat
    22621 gaagatgaga tggtacgtca ggttgccttg ggacgcttgt ggttagcaga aaatcagacg
    22681 attattgacg gattgagaac tatttttgaa gcatatcaaa gcaatattgc aagtattcca
    22741 gcagctgttc gtcgcttggt tttagccaac caaatgaaat attttgaaac agatagcttg
    22801 gttgatattt attttgagac ttatgtagca actactgata ataatttacg cagtgattta
    22861 acggttgctt ttagtcaaac aaaacaatca actactatta gacgtattct tgttagttta
    22921 aaagataaag atattattaa acctcaggat ttatcttatt ggtacaatgc tttgcttgga
    22981 caaagcttta ctcaagatat catttgggaa tgggctagag aaaattggga ttggattaaa
    23041 tctgcacttg gtggagatat gagctttgat aaatttgtta tttatccagc aagtaacttt
    23101 aaaacaccaa aacatctagc agaatataaa tctttctttg aacctaagct agatgatatg
    23161 gctattagcc gtaatattac catgggaatt aatgaaattg aagcacgtgt tgctttgatc
    23221 actaaagaaa aagaagcagt tattgcagct ctcagtcatt actagaccat aagcaaaaga
    23281 gttgggggac tttccagctc ttttttgtag taagctagct tcctttctta gttccgtcta
    23341 taactaaatg attatccata gtttaattca aaaggagatt gcactttaaa gcgaaggcta
    23401 ttagcctctt aaatactttt ttacca
//
LOCUS       DAUGHY010000026        23329 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.026, whole genome shotgun sequence.
ACCESSION   DAUGHY010000026 DAUGHY010000000
VERSION     DAUGHY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23329
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.026"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            161..643
                     /locus_tag="VH752_001038"
     CDS             161..643
                     /locus_tag="VH752_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEQ8289400.1"
                     /translation="MDWRWWKIAYEELTEKQQRFVDKYITTFNATESAKQAGYSEKSA
                     YSQGQRLLKNVEIQKAMKERFLEAKDTKGDRIQDVAETLEQDTSIARGEIQISEFKET
                     DMLTGQVVIHTKREYTPSHEEQGRARDRIYKVNGAYSEKRELEHSGTVVFANEDNIPD
                     "
     gene            621..1910
                     /locus_tag="VH752_001039"
     CDS             621..1910
                     /locus_tag="VH752_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEQ8289401.1"
                     /translation="MKTTSLIKVDLPSTIGIGYGAFWRSRNFYRVVKGSRGSKKSKTT
                     ALNFIVRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNQLKVTHLFKFNESLPEITV
                     KATGQKILFRGLDDELKITSITVDVGALCWAWFEEAYQIETEDKFSTVVESIRGSLDA
                     PDFFKQITVTFNPWSERHWLKRVFFDEETKRADTFSGTTTFRVNEWLDDVDKRRYEDL
                     YKTNPRRARIVCDGEWGVAEGLVFDNFEVVDFDVEKTIQRVKETSAGMDFGFTQDPTT
                     LICVAVDLANKELWLYNEHYQKAMLTDHIVKMIRDKNLHRSYIAGDSAEKRLIAEIKS
                     KGVSGIVPSIKGKGSIMQGIQFMQGFKIYIHPSCEHTIEEFNTYTFKQDKEGNWLNEP
                     IDKNNHVIDAIRYALEKYHIRSNESNQFEVLRAGFGY"
     gene            1922..3424
                     /locus_tag="VH752_001040"
     CDS             1922..3424
                     /locus_tag="VH752_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ8289402.1"
                     /translation="MYTESFRDSTGKTKTLEFRFHREARMRYQAESLESLLTEKYKLL
                     REMIEHHDKVQKPRIQELLDYAEGNNHTISEIGRRKDDDMADVRAVHNYGKYISTLKQ
                     GYLVGNPIRVEYIDGTEQQQDLLKDLSVKNNFHQLNRRLVKDLSKVGRAFELIYRSMD
                     DKTEVVRLDPREVFVIYQNNLEQSSLAGVRYYNKNQLDGTTKIVELYTDNKILKFEYD
                     GDLTPIGETSSHAFGSVPITEYLNTDDGMGDYETELSLIDLYDAAQSDTANYMQDLSD
                     AILAIIGRVSFPGYVDTAEKAIEYLRKMRKARLLNLEPPVDQDGREGSVDAKYLYKQY
                     DVQGTEAYKNRIVSDIHKFTNTPDMTDSKFAGQQSGEALKWKVFGLDQERVDMQALFE
                     QSLKRRYKLIARVSQLLKEIDDFDISKLKITFTPNLPKSLQEKIEAFKALGGELSQET
                     AMAITDIVEDAKKEISLINSESKSRSQLAQKLEETSRLTDRELAHDHQKE"
     gene            3405..4967
                     /locus_tag="VH752_001041"
     CDS             3405..4967
                     /locus_tag="VH752_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEQ8289403.1"
                     /translation="MTTRKSKYWRDRIKKEMDAKEADDISLEQSMKQLHDYHFRNIEK
                     EIESFYQRYADKEKIDLSEARKRASELDISAYQKKAKELVAKAEKLRKEGRTVTRDDF
                     THQENADMSIYNLAMKTNALELLRLNIDLEMQELANGEHKLTKKFLDEGYRKETEFQA
                     GLLGLSVASQASVKSLADAVINANFKGAKWSDNIWDRQDKLRSIISQSVQSAILRGKN
                     GLTIARDIRREFDVSASYAKRLAITEHARVQMEVGRLSMAENGFAMFDILPEPKACDV
                     CKDIAKHGPYHLDKWIIGENSPPFHPYCRCAVVGLDKKQEEKVNDKSEKFENITPNLM
                     PLFSKFASKITDLQRKIVYSADLADTGYIRTPHAFDINNTLRNKGYNYLNVDDKLITD
                     TLDSVISINSTPKNIKVYRFDDFELLGSINEQNNNIFDSGNFMDKLNQGGLSYTNDGY
                     TSASYDVKKNVMGYRPIKTEIKVPKGSHVYLTDNEEESEIILPRGTKYDIINAKINEY
                     EEIEITMEIRKE"
     gene            4971..5156
                     /locus_tag="VH752_001042"
     CDS             4971..5156
                     /locus_tag="VH752_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289404.1"
                     /translation="MDFSDFLNKKQKEWDESHPIPDFSAMSDEELLYQPMSEALVSER
                     FAKELSKEVRKRNLLSK"
     gene            5542..5808
                     /locus_tag="VH752_001043"
     CDS             5542..5808
                     /locus_tag="VH752_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289405.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAMQAKALRNQNRRIAELRAIIQ
                     QNTQAINREFVTAKNTILDNQLAIKSIGDDVNHIKQNYKRKWRK"
     gene            5952..6485
                     /locus_tag="VH752_001044"
     CDS             5952..6485
                     /locus_tag="VH752_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEQ8289406.1"
                     /translation="MAEETQVVETVEEQVVPEAKQPQDEKKYTDADVDAIIDKKFAKW
                     KSEQEAEKSEAKKMAKMNEKEKADYEKQKLLDELQELKNDKTRNELTAVARQMFAESE
                     INVNDDVLGLVVTLDAEQTKANVTTLANAFAKVIADDRKALVRQTTPSTGGGIAKQTN
                     YGANLASKAAQQSTKLF"
     gene            6495..6875
                     /locus_tag="VH752_001045"
     CDS             6495..6875
                     /locus_tag="VH752_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEQ8289407.1"
                     /translation="MNKRKVTTSKEILHNLDYEAISVTLDSNKIGKKVVPAGTILAGK
                     DKSIFEDRKQKVETVTNEEVSTKEYVDGILFTDVDLTNGDAVGSCVYRGTINADKLAD
                     SSVAENYDDLEEVLPHIVFIKGGK"
     gene            6878..7927
                     /locus_tag="VH752_001046"
     CDS             6878..7927
                     /locus_tag="VH752_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEQ8289408.1"
                     /translation="MALIHEIITSENIKGFYNAKNENVENTLGEKAFPPKQQLGLKLS
                     FIKGAAGKPVTLKAAAFDTKVPLRDRMAVELLDEKMPFFKEAMSVKEADRQQLNILSQ
                     TKNNELIDTILASIYNDQATLIAGAKARLEAMRMEVLSKGKIHIQSNGVMKDIDYGLA
                     EDQTTKPDAKWDSAGTATPLKDIEKAIEKMAERGFVPEAIIMNSKTFSLIKNAESTLD
                     VVKPMAPNGAAVTKRDLNTYLEDELQIKVILKDGMFVGDDGESRKYFPDGFATLVPNG
                     NLGYTVFGTTPEQSDLLGGEATDANVSIVETGIAITTTKTTDPVNVQTKVSMIALPSF
                     ERLEEVHIINGLQDA"
     gene            7939..8205
                     /locus_tag="VH752_001047"
     CDS             7939..8205
                     /locus_tag="VH752_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289409.1"
                     /translation="MAKVIKTFRDKVTTNAYYVGDDYKGDRIEELTANGYLAGNTPKL
                     DTVEEVDLDKLKSDEIKAKLDELGIEHDSKLKKAELLELLKANI"
     gene            8217..8570
                     /locus_tag="VH752_001048"
     CDS             8217..8570
                     /locus_tag="VH752_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEQ8289410.1"
                     /translation="MANVNTFSIVKNVKLDLGIEDDNQDQLLEMLLNRITDHFKANYG
                     VLEIDNAFSFVLEDCLIARFNRRGSERAKTEEVEGHKTTYYDHLNEFEPYDAMIMTKL
                     NLIKDKSRKGGLYFL"
     gene            8567..8875
                     /locus_tag="VH752_001049"
     CDS             8567..8875
                     /locus_tag="VH752_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289411.1"
                     /translation="MRYADRVTFVKTTDEQYNPDLGEYTHTEVISITKPCFVMDMGME
                     KSVQIFGDYQKDRKVIYLKQPYTKAFDYCEYEGRRYKTQANKLGAIVFYLEGDDSIGG
                     "
     gene            8856..9221
                     /locus_tag="VH752_001050"
     CDS             8856..9221
                     /locus_tag="VH752_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEQ8289412.1"
                     /translation="MTLLVADISLKVVGTAGLKKKLELIIKKDAVKKIVRDNGTQLQR
                     KMINKAVFTKGYSTGATRRSITMQIGDGGLSVKVKPGTHYAGYLERGTRLMSKQPFVL
                     PALKEQKVKFRKDLEALVK"
     gene            9218..9607
                     /locus_tag="VH752_001051"
     CDS             9218..9607
                     /locus_tag="VH752_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEQ8289413.1"
                     /translation="MIKTRDQSIFDEMFKRIQSLGFTVYDYKPMTEVPYPFVEMESTD
                     AEYIPNKDDIKGSVELMLSVWGMQKKRKQVSDMASAIFSQALTVESSDVFRWSLNTRQ
                     SSIQMLDDTTTVTPLKRAIVTLRFNLR"
     gene            9617..10270
                     /locus_tag="VH752_001052"
     CDS             9617..10270
                     /locus_tag="VH752_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEQ8289414.1"
                     /translation="MPESETLSPDIHTKSHEDSPNTVKKQEEVMETQLEAKQGIHSIL
                     LFRLLKEASSEAATKLAFQTEHEVGKSRDVDGQKTKDGIIQSVGALEYDFKATSILAK
                     GDVLAAKLEKAMENGELVEIWDIDLEETSKNGDSDNKLANVWGIDKNGTNRGNGKYLA
                     TYYQGYISSFSAKKNAEENIEIEMEFAINGVGQKGFATLTDAQKAAVQYAFKDTTKG"
     gene            10330..10683
                     /locus_tag="VH752_001053"
     CDS             10330..10683
                     /locus_tag="VH752_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HEQ8289415.1"
                     /translation="MQLEIKGKTHNVKFGTRFVAEMDKNHIAERQGFKFGAGLQSSVP
                     FLIDHSVVTLAEVIYTGTITEPPRPSLNDIYDYIDEVEDIEKLFDDVLDELRQSNASK
                     LFMAQVEKDLAEAKA"
     gene            10725..11054
                     /locus_tag="VH752_001054"
     CDS             10725..11054
                     /locus_tag="VH752_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289416.1"
                     /translation="MIVLNCIRYLGMTDINEIGRLTLYEYDLLMTGKALAAVDESHKA
                     HKQAWINHQVTATKLVGGKKNKKEVPVYKKFKDFFDYEEEIRKITQEIDEGYDKKGMD
                     LLLKANL"
     gene            11069..14704
                     /locus_tag="VH752_001055"
     CDS             11069..14704
                     /locus_tag="VH752_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEQ8289417.1"
                     /translation="MGESYSVEAVLTAVDKTFGKTLQSAIRSIDGLEKRSTGFSSVSQ
                     KASSMFKSMLGANLAGQAISAMTRTVSSGLGSMLGEMNSSAKAWKTFDANLADIGFGK
                     KQILAAKTAMQDYATKTIYSASDMASTYAQLAAVGVKDTGKLVKAFGGLAASAENPKQ
                     AMKSISQQMTQAVGRPTVAWQDFRIMLEQAPAGMAKVAKSMGKNLDELVADIQAGRVK
                     TSDFLEAVKKAGNDKSFQKMATEFKTVDQAIDGMREGLSNKLQPAFEKVNQFGIRAIE
                     AIGKQLDKVDFSKFASNLGKFLEGINIDKIVSNISSAISSVTSKVKEFWGGFKQTGAI
                     SAFSGALKSVWGALKNVASAMSGGSWKNFGSIVGGIVKHVSNFAKAIADVVGKMEPGR
                     LQSWIATFAAVGGGLKLFEKLTGQSVVGSFLDKISTKFGLFGKKAKEGTDQAANGSRK
                     SGGIISQIFNGLGNIVKSAGTAISTAAKGIGTGIKTALSGAPPIISSLGTAISTVAQG
                     IGTGLAIAFRGLGAAIAMVPPTTWLALGTAILMVGAAFALAGTQADGISQILRTIGDV
                     VVQVLQQVTDSLATLLTIIANAIGSMLPIVAGAISQIVGAVAGGLSQLIIAVSTGVSL
                     VIGAFTGLLGGISGVINSISAVIQSLTGVITAVFNGIATVISSVGSTIKDVLTGLGTA
                     FEGFGNGVKSALEGVGAVIESFGSAVRNVLDGVANILDSMGTAALNAGRGVKEMAKGI
                     KMLVDLSLGDLVATLAAVASGLGKMASSAGEMTTLGSAMSKVANGMTRLATSATIAIT
                     GLTVFATTMATIKTAVATLPPVLTMAASGFTTFTTQAVAAVTGLAAINAPITMFKAQL
                     MTITPALAQAGAGFAAFVAQSSTFSTGLASAGPTIAAFNANLMSLSATTGVLVASIAG
                     LSAVLSVVSAGFSQIGASATATVGQIQAFASSTTVVSSAFASMQSMIQSAMAAIVSSI
                     ITSFNQAASQMQSILSRMLSQARTFGSQLEQQMRQSGQRSGQNLARGLSSQQGAVINA
                     ISSMVNAAVSRANAGAGPMRQAGAYIGQGLAQGMYSALGAVTAAANALVAQAERAARA
                     KAMIHSPSRLFAKRVGQYIPQGVAMGIDKNADVVDDSVGGLFDSINSFDFNIADRLTS
                     IGAKFQGVVKSESSQSLSQQQEFVHTAQPAYINFSLGGNEYEAFVSDITNQQAKIEKI
                     RLKRSSW"
     gene            14737..15516
                     /locus_tag="VH752_001056"
     CDS             14737..15516
                     /locus_tag="VH752_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEQ8289418.1"
                     /translation="MYEFNDTIRGTPKVTFNLKTTIGGKVLEDELNNGFGTFRTLTVS
                     GRDVVDLEHQTTSVLGRNGEYFHNATVEVRKLEIKAKITGKDNQSMRLQYEKLNRLIV
                     SHNQVFLSFSDEPDRNYLGIFKSKVVPEEVSNEQIIGLTFICYNPFKMSDVKTKKGTS
                     IQNGGLFQTKPIITLNLSSPTKEIKLLHVESQKYIRLTGTYTTDEIKIDMATGKITQN
                     GRNILGNLDMVNSRYFELLPGKNTLQCDNATITAEFREVYL"
     gene            15513..17564
                     /locus_tag="VH752_001057"
     CDS             15513..17564
                     /locus_tag="VH752_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ8289419.1"
                     /translation="MIYLFDKLERLVATVGTDDLLSWHFKVKNNDWDQASFEVPVDYD
                     IEPFVYFGFFNYDPNQKEDVFKLFKVIDYNLEDSKFYKGLDKAESDLDTIAIIKDKRF
                     RQSSADACIDGALEGTGYQVGKVEGITNVRTLSYYYISPRAALIKIVEAFNCEFNVRY
                     TFINNKITSRYIDLKKRFGKPTGKQFEHGNNLLKIVYEESTDDIVTCLIGRGKGEEIQ
                     HEEAEPKDVEGHLPQEERRQGYGRRIEFTDVVWSVEKGDPIDKPAGQNFVALDSAREE
                     YGLSQNGELKHRWGVFVNEEIEDKTELLKATWEELQRLSIPIRIYKAEILDIGPETWK
                     GDSVAIIYDEVKIAFETRVDEIDIDKLNFNRSVVTLGDYSVVQNRESRSRKEAVQNMI
                     DESLETITDLGMTFQEFLQGIEKRIETGKKEMEDNWRKVNLEFDNFKKKVEQEGLQFN
                     TLKEQIKEVDERTDKELEEFRATLKNLALPEEAIKKITETIKVDDIPSLKQNFDDLKN
                     KVSETSETARLNAEIIGTDGKTRYNKNLLVGDPNRTKTYDEDYIEVEANDGGFKRGET
                     YTISFSQTCELLKKVAVTLTQANNKGVKLVLTPTKAKMEAQTFEVAKDKQSIEVYPLS
                     YTAVLTGDWYKSKQIDLNASEVQELALEMAYKDVVDGNNATIAGQWSDSPQMILDGGK
                     K"
     gene            17561..18682
                     /locus_tag="VH752_001058"
     CDS             17561..18682
                     /locus_tag="VH752_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
                     /protein_id="HEQ8289420.1"
                     /translation="MTETIPLRVQFKRMKAAEWASSDVVLLEGEIGFETDTGFAKFGD
                     GQNTFSKLKYLTGPKGPKGDTGLQGKTGGTGPRGPAGKPGTTDYDQLQNKPDLGAFAQ
                     KEETNSKITKLESSKADKSAVYSKAESKIELDKKLSLTGGIVTGQLQFKPNKSGIKPS
                     SSVGGAINIDMSKSEGAAMVMYTNKDTTDGPLMILRSDKDTFDQSAQFVDYSGKTNAV
                     NIVMRQPSAPNFSSALNITSANEGGSAMQIRGVEKALGTLKITHENPNVEAKYDENAA
                     ALSIDIVKKQKGGKGTAAQGIYINSTSGTTGKLLRIRNLNDDKFYVKPDGGFYAKETS
                     QIDGNLKLKDPIANDHAATKAYVDGEVEKLKALLAAKQM"
     gene            18695..20608
                     /locus_tag="VH752_001059"
     CDS             18695..20608
                     /locus_tag="VH752_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEQ8289421.1"
                     /translation="MSRDPTLTLDESNLVIGKDGRVHYTFTASQDNQTVRLASNCLGT
                     AHFNQLMIERGDKATGYVAPVVVEGSGESTGVFKSLEEMLSGLQSINLELTDTKNSNL
                     WSKIKLTTNGMLREYHRDNITTEIIEKADGIATRISEDSDKKLALINDTIDGIRREYQ
                     DADRELSASYQAGIEGLKATMASDKLGLQAEIKMSARGLSQKYDDEIRKLSAKITTTS
                     SGTTEAYENKLEGLRAEFTRSNQGMRIELESQISGLRAVQQSTASQISQEIRDRTGAV
                     SRVQQDLDSYQRRLQDAEDNYSSLTHTVRGLQSDVVSPTGKIQSRLTQLAGQIEQRVT
                     RDGVMSIISGAGDSIKLAIQKAGGINAKMSGNEIISAINLNSYGVTIAGKHIALDGNT
                     TVNGTFTTKIAEAIKIRADQIIAGTIDAARIRVINLNASSIVGLDANFIKAKIGYAIT
                     DLLEGKVIKARNGAMTIDLQSGQINHYTNESAMRRIDSSTASQFIKMTKSGFISEIGN
                     MQAAMTVIGSNSDGSENHENKTFGGIRIWNGKSSYQSTSFVELVGNRVAIYGNKNRSP
                     WLFDSTTSGYAYLIPQNDRGIKHVIGRADRKIDQIHVGDIYVQGERVAMMLKDLALKI
                     GYIGTGGWANRIG"
     gene            20622..20780
                     /locus_tag="VH752_001060"
     CDS             20622..20780
                     /locus_tag="VH752_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289422.1"
                     /translation="MQEKLLGKIINDLALKVANLTLENAQLKAQHEIELEELNAQDEA
                     TAPKEEGK"
     gene            20783..21394
                     /locus_tag="VH752_001061"
     CDS             20783..21394
                     /locus_tag="VH752_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEQ8289423.1"
                     /translation="MRNWKVTGKYPQLDGTGAVASTHIIIAAEDGAVIPQLIKQDLTS
                     TNDTEIIKAALEEFKKSEYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQRMINLQTIHVLTSGGNVEPDIYKGMLELIEPAKQGEYQAYDVFTVVDESHEDQ
                     AVEGNLVFVHVNEPFTYEAQTLKDLESEDKVTVIKYADLVKQD"
     gene            21405..21701
                     /locus_tag="VH752_001062"
     CDS             21405..21701
                     /locus_tag="VH752_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289424.1"
                     /translation="MIDFVQIGAFCGAALSILGVWGFIVNPFKKAMEANEFAMAQLKD
                     SIKELAYELKNLDREREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            21698..21883
                     /locus_tag="VH752_001063"
     CDS             21698..21883
                     /locus_tag="VH752_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289425.1"
                     /translation="MNNWFKKVAIKTIKTMAQTAVGLIGSSVLITDINWPTMLSAVLL
                     SGLTCILMNVSQIKEEE"
     gene            21969..23258
                     /locus_tag="VH752_001064"
     CDS             21969..23258
                     /locus_tag="VH752_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEQ8289426.1"
                     /translation="MHIDGSLGAQCWDGYADYCKYLGLPYANCTNTGYARDIWEQRHK
                     NGILNYFDEVETMQAGDVAIFMVVAGVTPYSHVAIFDSDAGSGYGWFLGQNQGGANGA
                     YNLVKIPYSTTYPTAFRPKVFKNAVTVIGNIGLNKGDYFIDVSAYQQADLTATCQQAG
                     TTKTIIKVSESLAWLSDRHQQQANTSDPIGYYHFGRFGGDSNLAQREADLFLSNLPTK
                     KVSYLVIDYEDSASADKEANTNAVIAFMDKIANAGYKPVYYSYKPFTLNNIDYQQIIA
                     KYPNSIWIAGYPDYEVRTEPLWEFFPSMDGVRWWQFTSVGVAGGLDKNIVLLADDSSK
                     VDIPKIDKPQSQLTFNQKLDTNTKLDNSNVPYYEATLRTDYYVESKPNASSADKEFIK
                     AGTRVRVYEKVNGWSRINASQSDQWVEDKYLSNATQV"
BASE COUNT         7896 a         4106 c         5241 g         6086 t
ORIGIN      
        1 tactgattta atttcaattt tagtagtttc aagttggttg agctcaacag taatgatgtt
       61 cctaagttcg ttctttgtgc taagaacatt aattttgtta gttgaaactt ataataattt
      121 caaaaataaa taggtcatca cattgtggtg gctttttatt atggattgga ggtggtggaa
      181 aatcgcatac gaggaattaa ccgaaaaaca acagcgtttt gtggataagt acatcaccac
      241 atttaacgct actgagtctg caaaacaagc tggatattct gaaaaaagcg cttatagcca
      301 aggtcaacgc ttgttgaaaa atgttgaaat tcaaaaagca atgaaggaac gttttttgga
      361 ggcaaaagat actaaaggag accgtattca agatgttgca gaaacgttgg aacaagatac
      421 atcgatagcc cgtggagaaa tccaaatatc tgaattcaaa gaaacagata tgctgacagg
      481 tcaagtggtt atccacacga aaagagaata taccccaagt cacgaagaac agggtagggc
      541 tagggataga atttataaag ttaatggagc ttactcagaa aaacgtgaat tagagcattc
      601 tggaacggtg gtgtttgcaa atgaagacaa catccctgat taaagtagat ttgccatcaa
      661 caatcggtat aggttatggc gctttttggc ggtctagaaa tttttatcga gtagttaaag
      721 gcagccgtgg atctaaaaaa tctaaaacga ctgctttaaa ttttatcgtc agactgctga
      781 agtacccttg ggctaactta ttggtcatcc gtagatactc aaacactaac aaacaatcta
      841 cttataccga ttttaaatgg gcgtgtaatc aattaaaggt tacacacctt tttaagttta
      901 atgagagttt gccagaaata actgtaaagg caacgggcca aaagatactg ttccgtggac
      961 ttgatgatga gttaaaaatc acatctatta ctgtcgatgt tggcgctttg tgctgggctt
     1021 ggtttgaaga ggcttatcaa attgagaccg aagataagtt ttcaacagtt gtcgaatcaa
     1081 tccgcggtag tttagatgct cctgattttt ttaaacagat aacagtcacg tttaacccgt
     1141 ggtcagaaag acattggctt aaacgtgtct tttttgatga agaaactaaa cgggctgata
     1201 cattttctgg gactacaaca tttagagtaa acgaatggct tgatgatgtc gataaaagac
     1261 gctacgaaga tttgtacaag actaatccaa ggcgggctag aatcgtgtgc gatggtgaat
     1321 ggggcgttgc tgaaggtctt gtttttgata actttgaagt cgtagatttt gatgttgaaa
     1381 aaacaattca acgcgttaaa gagacctcgg ccggtatgga ctttgggttt actcaagacc
     1441 ctacaactct tatatgtgtt gcagttgacc tcgcaaacaa agagttatgg ctttacaacg
     1501 aacattatca aaaggctatg ttaacagatc atattgtcaa aatgataaga gataaaaact
     1561 tgcataggtc ttacatcgca ggggatagcg ccgaaaaacg cctcattgca gaaataaaaa
     1621 gtaaaggggt gtctggaatt gtcccgagta ttaaaggtaa agggtcaatc atgcaaggga
     1681 ttcaattcat gcaggggttt aagatatata ttcacccatc ttgcgaacac acaatagaag
     1741 agtttaatac ttacactttt aagcaagaca aagaaggtaa ttggttaaac gaaccgatag
     1801 ataagaataa ccacgttatt gatgcgatta gatatgcgct tgaaaaatac catatcagaa
     1861 gcaacgagtc aaatcagttt gaagttctta gggctggttt tggttactag aaaggaaaat
     1921 aatgtacaca gaatcattta gagatagtac gggaaagact aaaacattag agtttaggtt
     1981 ccaccgtgaa gctcgcatga ggtatcaagc ggaaagtcta gaaagcttgt taaccgaaaa
     2041 atataagcta ctccgtgaaa tgattgaaca ccacgataaa gtccaaaaac cacgcataca
     2101 agagcttcta gattatgcag agggaaataa ccacaccatc agcgaaatag gccgtaggaa
     2161 agacgatgac atggctgatg ttcgtgctgt gcataactat ggtaagtata tttcaacgct
     2221 caaacagggc tatttggtgg gtaatcctat tcgtgtagag tatattgatg gtaccgagca
     2281 gcagcaagac ctattaaagg acctatctgt taaaaacaat ttccaccagc tgaaccgcag
     2341 attagtaaaa gacctatcca aggttggtcg agcgtttgaa ttgatttatc gcagcatgga
     2401 tgacaagaca gaggtcgtta gactagatcc acgggaagta tttgttatct atcaaaataa
     2461 cctagagcaa tcaagcttag ctggtgtgcg gtactataac aaaaatcaat tagatggtac
     2521 tacaaaaatt gtcgagcttt acaccgataa taaaatcctg aagtttgaat atgatggtga
     2581 tttaacacct atcggagaga cttcctctca tgcgtttggt tcggtgccaa tcacggagta
     2641 cctcaacaca gatgacggca tgggtgacta cgagacagag ttgtctttaa tcgacttgta
     2701 tgatgcagcg cagtctgaca cagctaacta catgcaggat ttgtcagacg cgattctggc
     2761 aatcattggt cgtgtatcat tccctggcta tgtcgacact gccgaaaaag ccattgaata
     2821 cttacgtaag atgcgtaaag ctagattgct taacctcgag cctcctgtcg accaagacgg
     2881 gcgtgaggga tctgtagatg ccaaatatct atataaacaa tatgacgtac aaggaaccga
     2941 agcctataaa aatcgtattg tgtccgacat ccataaattt accaatacgc cagacatgac
     3001 agacagtaag tttgccggtc aacaatccgg agaggcgttg aagtggaaag tgtttggtct
     3061 tgatcaggag cgtgtcgaca tgcaagcttt atttgagcaa tctcttaaac gtaggtacaa
     3121 actaatcgct cgtgtaagcc aactgcttaa agagattgat gattttgaca tcagcaagct
     3181 taaaatcaca tttacgccaa acctacctaa gtcgctacaa gaaaagattg aagcctttaa
     3241 agcattgggt ggagagttgt cgcaagagac agctatggct attacagaca tcgtggaaga
     3301 tgctaagaaa gaaattagcc ttatcaacag cgagtcgaaa tcacgtagtc aactagcgca
     3361 gaagttagaa gaaaccagta gattgactga tagggagtta gctcatgacc accagaaaga
     3421 gtaaatactg gcgtgaccgt atcaagaaag aaatggatgc taaagaggca gacgatatct
     3481 ctcttgagca atccatgaag caattgcacg attatcattt caggaatatc gaaaaagaaa
     3541 ttgagtcgtt ttatcaacgt tatgctgaca aagagaaaat agacctttca gaagcccgta
     3601 agagagcttc tgagcttgat atttctgctt accagaagaa agctaaggaa cttgttgcaa
     3661 aggctgagaa gctacgaaaa gaggggagaa cggtaacaag agatgatttt acccaccaag
     3721 aaaatgcaga catgtctatt tacaacttag ccatgaaaac gaatgctttg gaactattgc
     3781 gcttaaacat tgatttagaa atgcaagaac ttgccaacgg cgaacacaag ctaaccaaga
     3841 aatttcttga tgaaggctat cgcaaagaaa ccgagtttca agctgggcta ttaggattat
     3901 cagttgctag ccaagcgagt gtgaaaagct tagctgatgc cgttatcaat gctaatttca
     3961 aaggagcaaa atggtcagat aacatttggg acagacaaga taagttacgc agtatcatat
     4021 ctcaaagtgt tcagagtgct atcctaagag gtaaaaatgg cttaactatt gcaagggata
     4081 tcagacgaga atttgatgtg tcagcgtctt acgcaaagcg actagcgata acggagcatg
     4141 caagggttca gatggaagtt ggtagattat ccatggcgga gaatggcttt gctatgtttg
     4201 atatattgcc tgagcctaaa gcatgtgatg tttgcaaaga tatagctaag catggtccat
     4261 atcaccttga caagtggata ataggggaaa actctccgcc gtttcatccg tattgtcgtt
     4321 gtgcagttgt aggattggat aagaaacaag aagaaaaagt gaatgataaa tctgaaaaat
     4381 ttgaaaacat aacaccgaat ctcatgccgt tatttagtaa gtttgcaagt aaaataacag
     4441 atttgcaacg taaaatagtg tactctgctg atttagcaga taccgggtac attagaacac
     4501 cgcatgcgtt tgatataaac aataccttgc gaaataaagg ctacaattat ctaaacgttg
     4561 atgataaact aattaccgac acattggata gtgtcatctc gataaattca actcccaaaa
     4621 atataaaagt ataccgcttt gatgactttg agttattagg ttcaatcaac gaacaaaaca
     4681 ataatatttt tgattcaggt aattttatgg ataaattaaa ccagggagga ttatcataca
     4741 ccaacgatgg atatacttct gcaagttatg atgttaaaaa aaacgtgatg ggatatcgtc
     4801 ctattaagac tgaaataaaa gtacctaaag gaagccatgt ttatcttaca gataatgagg
     4861 aagaaagtga aattatacta ccacgaggga caaagtatga tataattaat gcgaagataa
     4921 atgaatatga agaaatagaa atcactatgg aaataagaaa ggagtaagcg atggattttt
     4981 ctgatttttt gaataaaaaa caaaaagagt gggatgaatc tcatccaatt cctgacttta
     5041 gtgcaatgag tgatgaagaa ttgctttatc agccaatgag tgaagcttta gtgtctgaaa
     5101 gatttgctaa agagttatct aaagaggtta gaaagcgtaa tttattatca aaataaaaac
     5161 atattattat agacggaaga ctgtggtcgc ccgtcttttt ttgtacccaa aaacaggagg
     5221 aaacatgaat cgtgaaagta aaccaggtat ggaaagcgta aaaatcggat ggttgacata
     5281 ctctgttgaa aaggcatcgg acctgtaagg caaagatggc aattggggtc ttatacacta
     5341 caaaacacaa caaattaaac tagatgactc attaaccgag cagctcgaag atcaaacact
     5401 tatccacgaa attgttcatg gaattttagt tgaggcaggc tatactaacc acgaagagga
     5461 tcaagctaac cgcattggtc tgattttgta tcaagtattg caggataatg attttagctg
     5521 gttgtacaaa ggggaataaa tatgaataaa cgcatcaaga aaaaacgtaa gttagaaaca
     5581 gcagttgtga tgcttgttgc agaaaatgct atgcaagcaa aagctcttag aaatcaaaac
     5641 agacgaattg cagagctgag agcgattata caacaaaaca cccaagcaat aaatagagag
     5701 tttgtaactg ctaaaaatac gattttagat aaccaattag ctattaagtc aattggtgat
     5761 gatgttaatc acatcaagca aaattataag cggaagtggc gaaaataaat tttaaactgg
     5821 tcgaattcga cccctttaga atcaaactaa gtcgtaggaa tacggttttt ttattatgtc
     5881 caagcattga cgacgataaa agctatggaa ttaataatcg gggacgattt aaaacatagg
     5941 aggtgccaat catggcagaa gaaacacaag tagttgagac ggttgaagag caagtggtac
     6001 ctgaagcaaa acaaccgcaa gacgaaaaaa agtatacaga tgcagatgtg gacgctatta
     6061 tcgacaaaaa gtttgcgaag tggaagtcag aacaagaagc tgagaaatcg gaagccaaaa
     6121 aaatggctaa gatgaacgag aaagaaaaag cagactatga aaagcagaag ctgttagacg
     6181 aattgcaaga gctaaaaaac gataagacac gcaatgagtt aacagcagta gctcgtcaaa
     6241 tgtttgcaga atctgaaatc aacgtcaacg atgacgtact tggtttagtt gtgactttgg
     6301 acgcagaaca aacaaaagca aatgtaacaa cgctagcaaa cgcatttgct aaagttatcg
     6361 ctgatgaccg caaggctctt gtacgccaga ccactccgtc aacaggcggt ggtatcgcaa
     6421 aacaaaccaa ttacggtgct aacttggcta gtaaggcagc acaacaaagc accaagcttt
     6481 tttaggagga aattatgaac aaacgtaaag taacaacatc taaagagatt ctacacaatc
     6541 tcgactatga agctatttca gtaactttgg attcaaacaa aatcggcaag aaagttgttc
     6601 cagctggaac aattttggca ggcaaagata aatccatttt tgaagaccgc aaacagaaag
     6661 tagaaaccgt gacaaatgaa gaagtctcta caaaagaata tgtcgatggt attttattta
     6721 cagatgtcga tttaacaaat ggcgatgcag tcggttcctg tgtttatcgt gggactatca
     6781 atgcagacaa gcttgctgat tcatctgttg cggaaaatta tgatgatttg gaagaagtac
     6841 taccacacat tgtctttatc aaaggaggta aataaacatg gcattgatcc acgaaattat
     6901 cacatcggaa aatatcaaag gtttttataa cgctaaaaac gaaaatgttg aaaatacatt
     6961 gggagaaaaa gcattcccgc caaaacaaca actgggcctt aagttgtcat ttattaaagg
     7021 tgcagcagga aaaccggtta ctcttaaagc ggccgctttt gacactaagg tgcctctacg
     7081 tgaccgcatg gctgttgaat tgcttgacga aaaaatgcca tttttcaagg aagctatgtc
     7141 tgttaaagag gcagatcgtc aacaacttaa cattttgtct caaactaaaa ataacgaact
     7201 tatcgacacc atcttggcat ctatctacaa tgaccaggct acgcttattg caggtgctaa
     7261 agcacgcctt gaagcaatgc gcatggaagt actatcaaaa ggtaaaatcc atatccagtc
     7321 taacggcgtg atgaaagaca tcgactatgg attggccgaa gatcaaacga ccaaaccaga
     7381 cgccaaatgg gatagcgcag gaacagctac accacttaaa gacattgaaa aggctatcga
     7441 aaaaatggca gagcgtggtt ttgtgccaga agctatcatc atgaactcta agacatttag
     7501 tttgattaaa aacgcagaga gcaccttaga tgtcgtgaag cctatggcac caaatggggc
     7561 agcggttact aaacgtgacc taaacacata tcttgaagat gaactacaaa tcaaagtcat
     7621 cctaaaagat ggcatgtttg ttggtgatga tggtgaatct cgtaaatact tcccagatgg
     7681 ttttgcaaca ttagtaccta acggcaacct tggctacact gtatttggca caactccaga
     7741 acagtcagac ttgcttggtg gtgaagcgac agatgcaaat gtctcaattg tcgaaactgg
     7801 tatcgcaatc acaacaacca agacaactga tccagtaaac gtccaaacca aggtatctat
     7861 gattgccttg ccatcatttg aacgcttgga agaagtacat attattaatg gcttgcaaga
     7921 tgcataagga ggtgctttat ggccaaagtt attaagactt ttagagataa agtgacgact
     7981 aatgcttatt atgtaggcga tgattacaaa ggagatcgta tcgaggaatt gacagccaat
     8041 ggttacttgg ccggaaatac tcctaaactg gatactgtcg aagaagttga cctagataaa
     8101 ctcaaaagcg atgaaatcaa ggcaaaactt gacgaattgg gcattgagca cgattctaag
     8161 cttaaaaagg cggaattgtt ggagctttta aaagctaaca tttaacggag gtgtttatgg
     8221 caaatgtaaa cacatttagc attgtaaaaa atgtcaagct cgatttaggc attgaggacg
     8281 acaatcaaga ccagttgttg gagatgttgc taaatcgtat tacagaccac ttcaaagcaa
     8341 attatggcgt gttagaaatt gacaatgctt tttcttttgt cttggaagat tgtctgattg
     8401 ctcgttttaa ccgtagaggt tcagagcgtg ccaaaacaga ggaagtggaa ggtcataaaa
     8461 caacctacta cgaccatttg aatgagtttg aaccctacga tgccatgatt atgacaaagc
     8521 ttaatttaat caaagacaaa tctcgtaaag ggggactgta ctttttatga gatatgcaga
     8581 tagagttaca tttgttaaaa cgacggatga gcaatacaat cccgatttag gtgagtatac
     8641 gcacacagag gtcataagta tcacaaaacc ttgttttgtg atggacatgg gcatggaaaa
     8701 gtccgtacag atttttggag attatcaaaa ggatcgtaaa gttatctacc taaagcagcc
     8761 ttatacaaaa gcatttgatt attgcgagta tgagggcagg agatacaaaa cgcaggcaaa
     8821 taagcttggc gctattgttt tttatctgga aggagatgac tctattggtg gctgatatat
     8881 ctttaaaagt agttggaaca gctggtttaa aaaagaaact tgagcttatt atcaaaaaag
     8941 atgccgtcaa gaagattgtc agagacaatg ggacgcagct tcaaaggaaa atgattaata
     9001 aagcggtatt tacaaaaggc tattcaacag gtgcaactag acgttctatt accatgcaaa
     9061 tcggcgatgg tggattgagt gtcaaggtta aaccaggaac tcattatgcc ggctaccttg
     9121 aaagaggaac tcgccttatg agcaaacaac cgtttgtttt gccagctcta aaagagcaaa
     9181 aagtaaaatt tagaaaagat ttggaggcgc ttgtcaaatg attaaaacta gagatcagtc
     9241 tatttttgat gaaatgttta aacgcatcca gtctttaggc tttacagttt acgattataa
     9301 accgatgact gaagttccat atccatttgt ggaaatggaa tctactgatg cggagtatat
     9361 tccaaataaa gacgacatta aaggttctgt tgaacttatg ttgtctgttt gggggatgca
     9421 gaaaaaacga aagcaggtgt ctgacatggc atctgctatt ttttcgcaag ctttgacagt
     9481 agagagttct gacgttttcc gctggtcatt aaatactcgt caatcatcta ttcaaatgtt
     9541 agatgacaca acaactgtga cacctcttaa acgagcgatt gtcacactta gatttaattt
     9601 gagataggag gtagaaatgc cagaatcaga aacattatcc cctgacattc ataccaaaag
     9661 tcacgaagat agtccaaata ctgtaaaaaa acaggaggaa gtaatggaaa cacaattaga
     9721 agcaaaacaa gggattcatt caatcttgtt atttcgtttg ttgaaagaag catctagtga
     9781 ggcagcaact aaacttgctt ttcaaacaga gcatgaagta ggtaaaagcc gtgacgtaga
     9841 tggacaaaaa actaaagatg gtattatcca gtccgtgggg gctttggagt acgactttaa
     9901 agcaacatct attttagcta aaggtgacgt actagcagct aaactagaaa aagccatgga
     9961 gaatggtgag cttgtagaaa tttgggatat tgatttagaa gaaacaagca aaaacggcga
    10021 cagcgacaat aagcttgcaa acgtttgggg tattgataaa aatggaacaa acagaggaaa
    10081 cggtaaatac ctagcgactt attaccaagg gtacatctca agcttcagtg ctaagaaaaa
    10141 tgctgaggaa aatattgaaa ttgaaatgga atttgccatc aacggtgttg gccaaaaagg
    10201 attcgctaca ttaacagatg cccaaaaagc agctgtacag tacgcattta aagatacaac
    10261 gaaaggttaa tcaacaaggc cagttaatta ctggtctttt atttttaagt ttttaaagga
    10321 gaaagaataa tgcaattgga aattaaaggt aaaactcata acgtaaaatt tggtacacga
    10381 tttgttgccg agatggacaa aaatcatata gcagaacgtc agggatttaa atttggagct
    10441 ggtctacaat ctagcgttcc gtttttaatc gaccacagcg ttgttactct tgcggaagta
    10501 atttatacag gtacaatcac cgaaccacca cgtcctagtt tgaatgatat ttacgactat
    10561 attgacgaag ttgaagatat tgaaaagctt tttgatgatg ttttagacga actacgtcaa
    10621 tcaaacgcat caaagttgtt tatggctcag gtggaaaaag acctggcgga agccaaagcg
    10681 taacaaatat aaaaaagagt tattcttctc aagaaagctt tgagatgatt gtgcttaatt
    10741 gcattagata ccttggcatg actgacatca atgaaatcgg gcgattaact ttgtatgaat
    10801 atgatttatt aatgacaggc aaagcactag cggctgttga tgaatcacat aaagctcaca
    10861 aacaagcttg gataaaccac caagttacgg caacaaaact tgttggtggc aagaaaaata
    10921 aaaaagaagt ccctgtttat aaaaaattca aggacttttt tgattatgag gaagaaatcc
    10981 gaaaaatcac tcaagaaatt gatgaaggtt acgacaagaa aggtatggat ttacttctca
    11041 aagctaacct ttaaagaaag gaggttaaat gggagaatct tattctgttg aagcggtttt
    11101 gacagctgtt gataaaacct ttggcaaaac attacaatcg gcaatccgtt caatcgatgg
    11161 cttggaaaag cgttcaaccg gtttttcatc ggtgtctcaa aaagctagtt ccatgtttaa
    11221 atccatgtta ggagcgaatt tagccggaca agctatctca gcaatgacaa ggacagtgtc
    11281 atcaggcctt ggctctatgc ttggcgagat gaatagttca gcgaaagcgt ggaaaacttt
    11341 tgacgccaat ttagcggaca ttgggtttgg aaaaaaacaa attttggcag ctaaaacggc
    11401 gatgcaagac tatgcaacta aaacaatcta ctcggcatca gatatggcta gcacgtatgc
    11461 acagttagcg gcagttggtg tgaaagatac cggaaagctc gtaaaagctt ttggcggttt
    11521 agctgcatct gctgaaaacc cgaagcaggc catgaagtct atcagtcaac aaatgacgca
    11581 agcagtagga agaccaacag ttgcatggca agactttagg ataatgctgg aacaggcgcc
    11641 tgcagggatg gctaaagtcg ctaaatctat gggtaaaaat cttgatgaac tcgtcgccga
    11701 tatccaggcg ggtagggtta aaaccagcga ttttttggaa gcggtaaaaa aagcaggcaa
    11761 tgataagagt ttccaaaaga tggcaactga gttcaaaact gttgaccaag ccatcgacgg
    11821 tatgcgagaa ggcttatcca acaaattgca accagcgttt gaaaaagtga accaatttgg
    11881 aattagagcg atcgaagcaa tcggtaaaca actcgataaa gttgattttt ctaagtttgc
    11941 tagtaatctt gggaaattcc ttgaaggaat taatatcgat aaaattgtat ctaatatttc
    12001 atcggcgatt tcatctgtca cttcaaaggt taaagaattt tggggcggtt tcaaacaaac
    12061 tggagcaatt agtgcttttt caggagcttt aaaaagtgtt tggggagcgt taaaaaatgt
    12121 agctagcgct atgagtggag gcagttggaa aaactttggc tctattgtag gcggaattgt
    12181 aaagcatgtg tctaattttg caaaagctat tgctgatgtt gtcggtaaaa tggaacctgg
    12241 cagattgcaa agctggatag ccacttttgc agcagtcggg ggagggttaa agttatttga
    12301 aaagctaaca ggacaaagcg ttgttggctc ttttttagat aaaatcagta caaaatttgg
    12361 attatttggc aaaaaagcta aagaaggaac cgatcaagca gcgaatggct ctcgtaaaag
    12421 tggtggaatc atcagccaaa tctttaatgg cttgggtaat atcgttaagt ctgctggtac
    12481 agccatatca acagctgcaa aaggtatcgg tacagggatt aaaaccgcct tgtctggggc
    12541 acctcctatc attagttctc taggaaccgc aatatcaaca gttgcgcaag gtataggcac
    12601 tgggctagca atcgctttta gaggtttagg agctgcaatc gctatggtgc ctcccaccac
    12661 ttggctagct ttaggaacgg ctattttaat ggtaggagcg gcttttgcct tagcaggaac
    12721 tcaggctgat ggcattagtc aaattttaag gactattggc gatgttgttg tacaagtttt
    12781 acaacaggtc actgatagtc tagccacttt actaactatt atcgcaaacg ctattggctc
    12841 tatgttgcca attgtagctg gagctatctc tcagattgta ggcgcagtag cgggcggatt
    12901 atctcagctc attatagccg tttcaacagg ggtatctctc gttataggag ctttcacagg
    12961 acttcttggt ggtatttctg gggttattaa ctccattagc gctgttatcc aatcgctaac
    13021 tggtgtgatt accgcagtat tcaatggcat agctactgtt atttcatctg tcggttcgac
    13081 tatcaaagat gtattgacgg gtctaggaac cgcttttgaa ggatttggga atggtgtaaa
    13141 atcagctcta gaaggtgttg gggcagtaat tgaatcattt ggtagtgcag ttaggaatgt
    13201 ccttgacggt gttgcaaata tccttgattc tatggggact gcggcactta atgcaggccg
    13261 tggcgtcaaa gagatggcta aaggtattaa gatgcttgtt gatttatccc ttggagattt
    13321 ggttgctaca ttagcagctg tggcaagcgg tctagggaag atggctagct cagctggcga
    13381 aatgacaaca ttaggttctg ctatgagcaa agtagccaat ggtatgacac gtctagcaac
    13441 aagtgctacg atagcaatta ctggattaac agtctttgcc accaccatgg caactattaa
    13501 gacagcagtt gcaactctac cgccagtcct aacgatggca gcgagtgggt ttaccacatt
    13561 tactactcag gcggtggcag cagtgactgg attggctgca attaatgctc caatcactat
    13621 gtttaaagct caactaatga caataacacc agctctagca caagctggcg ctggctttgc
    13681 cgcgtttgtt gctcaatcat caacatttag tacaggttta gcatctgccg gtcctacaat
    13741 agcagcattc aatgctaatt tgatgagctt atctgcaaca acaggagtgc tagttgcatc
    13801 aatagctggt ttatcagctg tgctttctgt tgtatcagct ggctttagcc aaataggggc
    13861 ttctgcgaca gcaactgttg gtcaaataca agcttttgct tctagtacaa cagttgtttc
    13921 gtcagcattt gctagcatgc aatctatgat tcaatctgcc atggctgcaa tagtaagcag
    13981 cattataaca tcatttaatc aagcggcctc tcaaatgcaa tcaatcttat ctcgaatgct
    14041 atctcaggcc aggacatttg ggtctcaact agagcaacaa atgagacaat cgggacagcg
    14101 ttcaggacaa aatcttgctc gggggctatc ttctcaacaa ggtgctgtta ttaatgctat
    14161 ttctagcatg gttaatgctg cggtatcaag agccaacgcg ggagctggtc ctatgcgtca
    14221 agctggagcg tacatcggac aagggcttgc gcaaggaatg tattcagcgc taggagctgt
    14281 aacagctgca gcaaacgccc ttgtagcaca agccgagaga gcagcaagag ccaaggcgat
    14341 gattcattcg ccgtcaaggt tgtttgcaaa acgagttggt caatatatcc cgcaaggggt
    14401 agctatgggt atcgacaaaa acgctgatgt cgttgacgac tctgttggcg ggttatttga
    14461 tagcatcaat agctttgatt ttaatatcgc agatagactg actagcattg gagctaaatt
    14521 ccaaggtgtt gtcaaatcag agagttcgca atcgttatcg cagcaacaag agtttgtaca
    14581 tacagctcaa ccagcgtata taaactttag tttaggcgga aacgaatacg aagcatttgt
    14641 aagtgacatc actaatcaac aagcaaaaat tgaaaaaatc agactaaaga gaagcagctg
    14701 gtagttgttt ctcttagttt ttttgaaagg agtaaaatgt acgaatttaa cgatactatc
    14761 agaggtactc cgaaagttac ttttaattta aagacaacaa ttggtggaaa ggtattagaa
    14821 gacgaactca ataatggttt cggtaccttt aggacattga ctgtttctgg tcgtgatgtg
    14881 gtggacttag agcatcaaac aacaagtgtt ttagggcgaa atggtgagta tttccacaat
    14941 gccacagtcg aagtcagaaa actagaaata aaagctaaaa tcactggaaa agataaccag
    15001 tcaatgcgtt tgcaatatga aaaattaaac agattaattg ttagtcacaa tcaagttttt
    15061 ttatcatttt cagacgaacc tgacagaaat tatctaggta tctttaaatc taaagttgtt
    15121 ccagaagaag tttctaacga gcagattata ggactaacat tcatctgcta caatccgttt
    15181 aaaatgtctg atgtaaaaac taaaaaagga acatctatcc aaaatggtgg gttatttcaa
    15241 acaaagccta tcatcactct caacctatca tcaccaacaa aagaaattaa gttgttgcat
    15301 gtcgaaagcc agaagtatat cagattgact ggaacttata ccactgacga aatcaagatt
    15361 gatatggcca cgggtaagat tacccagaac ggacgcaata tccttggcaa tttagatatg
    15421 gttaacagtc gctattttga gctactacct ggtaaaaata ctttgcagtg tgacaatgcc
    15481 accataactg cagagtttag ggaggtttat ctatgattta tctctttgat aaactggagc
    15541 gattggtcgc cactgttggt actgacgatt tgctctcgtg gcattttaag gtcaaaaata
    15601 acgactggga tcaagctagt tttgaggtac cggtggatta tgatatagag ccatttgtct
    15661 actttggttt ttttaactac gaccctaatc aaaaagaaga tgtctttaag ctctttaaag
    15721 tcattgatta caacctagaa gatagcaagt tttacaaagg cttagacaaa gctgagagtg
    15781 atcttgatac cattgccatt atcaaagata agcgctttag acaatcgtcc gcagatgctt
    15841 gtattgatgg tgctttagag ggtacgggtt atcaagtcgg caaagttgag ggaataacaa
    15901 atgttagaac acttagttat tactacatca gtccacgagc ggctctgatt aagattgtgg
    15961 aagcttttaa ctgcgagttc aatgtcaggt atacctttat taataacaaa atcactagtc
    16021 gttatatcga cctcaaaaag cgctttggaa agccaacagg caagcaattt gaacatggca
    16081 acaacctgct aaaaattgtc tacgaggaat caacagatga cattgtgact tgtctgattg
    16141 gcagaggtaa gggtgaggaa atccaacacg aagaagctga gcctaaagat gtcgagggac
    16201 acttgccaca ggaagaaagg cggcaaggct acggtcgaag aatcgaattt actgatgttg
    16261 tctggtcggt cgaaaaaggc gacccgatag acaaaccagc tggtcaaaac tttgtagcac
    16321 tagatagcgc aagggaagaa tacggcttgt ctcaaaatgg tgagctaaaa caccgctggg
    16381 gtgtctttgt caatgaggaa atcgaggata agacagaact cttaaaagca acttgggaag
    16441 aattgcagcg tttgtcaatc cctatcagaa tttacaaggc agaaatctta gacattggtc
    16501 cagagacgtg gaaaggcgac tcagtagcca ttatctatga tgaggtaaaa atagcttttg
    16561 aaactcgggt tgatgagatt gatattgaca agcttaattt taacaggtca gtcgtaacac
    16621 ttggtgatta cagcgttgtc caaaatcgtg agtcacggtc tcgtaaagag gctgtccaaa
    16681 acatgataga tgaatcttta gagactatca cagacttagg gatgacattt caggaatttt
    16741 tgcaaggcat cgaaaaacgc atcgagactg gcaaaaagga gatggaagac aattggcgca
    16801 aagttaacct tgaatttgat aactttaaaa agaaggttga gcaagaaggc ttgcaattca
    16861 acaccttgaa agaacaaatc aaagaagttg atgaacgcac cgataaagag cttgaagaat
    16921 ttagagccac cctcaaaaac ctagcgttac cagaggaagc gattaaaaaa atcacagaga
    16981 ctatcaaagt tgatgacatc ccgtctctta agcaaaactt tgatgactta aaaaacaagg
    17041 tcagcgagac aagcgagacg gcacgtctaa atgccgagat tatcggtaca gacggtaaga
    17101 cccgctacaa caaaaatttg ctggttggcg accctaaccg caccaaaacc tatgatgagg
    17161 attacataga agtagaagct aacgacggtg gttttaagcg tggcgagacc tacacgatta
    17221 gctttagcca gacttgtgag ctactcaaaa aagtggctgt cacgctgaca caggctaaca
    17281 acaagggagt taagttagtg ctgacaccta ccaaggctaa gatggaagca cagacgtttg
    17341 aggtcgctaa ggataaacag tctatagagg tttatccttt gagctacacg gctgttttaa
    17401 ctggcgactg gtataaatct aagcagatag atttaaacgc gtcggaggtg caggaattgg
    17461 cccttgagat ggcttataaa gatgtggttg acggtaataa tgccacgata gcagggcagt
    17521 ggtcagacag cccgcaaatg attttagatg gaggtaaaaa atgacagaaa ctataccatt
    17581 aagagtccaa tttaagcgga tgaaagccgc cgagtgggca agtagtgatg tcgtcttact
    17641 ggagggtgag attggctttg agactgacac tggttttgct aagtttggcg atggtcaaaa
    17701 cacttttagt aagcttaagt accttactgg tcccaaaggt cctaaaggag acactggtct
    17761 ccaaggtaaa actggaggaa ctggtcctcg gggccctgct ggcaagcctg gaacgacaga
    17821 ttatgatcaa ctccaaaata aaccagatct aggtgcgttt gcacaaaaag aagaaactaa
    17881 tagtaaaatc accaaattag aatcaagcaa agcagataaa agcgctgttt actcaaaagc
    17941 agagtcaaaa atagagctag acaaaaaatt gagcttaaca ggcggcatag tgacaggaca
    18001 actacagttt aaacctaata aaagtggtat taaaccctca tcttccgtag gaggagcgat
    18061 taacattgat atgtctaaat cggaaggtgc tgctatggtg atgtatacaa ataaagatac
    18121 tactgatgga ccattgatga ttttacgttc tgacaaagat acgtttgatc agtcagctca
    18181 atttgtggat tacagcggta agactaatgc tgtaaatatt gtaatgcgcc agccaagcgc
    18241 acctaatttt tcctcggcac ttaatataac cagtgccaac gaaggcggta gtgcgatgca
    18301 aattagaggc gtcgaaaaag cgctaggaac gctcaaaatc acacacgaaa acccaaacgt
    18361 tgaggcaaaa tacgatgaaa acgctgcagc gttatctatt gatatcgtta aaaaacagaa
    18421 aggcggaaaa ggtactgctg cccaaggaat ctacattaac tcaacatcag gcacgacagg
    18481 gaagttgctt aggattagaa accttaatga tgataagttc tacgtcaagc ctgacggtgg
    18541 tttttatgcc aaggaaactt cgcagattga tggcaacctg aaactcaagg atcccatagc
    18601 gaatgatcat gcggcaacca aagcttatgt tgatggtgaa gtcgaaaaat taaaagcact
    18661 cttagcggct aagcaaatgt aaaaaggagg ataaatgagt agagatccaa cgttgacatt
    18721 agacgagtca aatctcgtta ttggtaagga tggacgtgtg cattatacat ttactgctag
    18781 tcaagacaac caaacagtta gactagccag caactgctta ggcacagcgc actttaatca
    18841 gctcatgatt gagcgagggg ataaagcgac tggctatgtg gcgcctgtgg ttgttgaggg
    18901 tagcggtgag tcgacaggtg tgtttaaaag tcttgaggag atgcttagtg gtctacagtc
    18961 tattaattta gagctgacag atactaaaaa ctccaatctt tggtcaaaaa tcaagctgac
    19021 gacaaacggc atgttacgtg agtatcatcg tgataacatc acaacggaaa tcatcgaaaa
    19081 agctgatggt attgccacac ggattagcga ggattctgac aaaaagctag cgctcatcaa
    19141 tgacaccatt gatggtatca gacgtgagta tcaagatgct gatagggagc tatccgcaag
    19201 ctatcaggca ggcatcgagg gactaaaagc cacaatggct agcgataaac ttggcttgca
    19261 agctgagatt aagatgtcag cccgaggctt atctcaaaag tatgatgacg agatacgtaa
    19321 gctatcggct aagatcacaa caacctcaag cggcacgacc gaggcctacg aaaacaagct
    19381 cgagggttta cgtgctgagt ttactcgtag taatcaaggc atgaggatag agctcgagtc
    19441 tcaaatcagc ggactaagag ctgtacagca gtcaacagct agccaaatct cacaagagat
    19501 tagagacagg acaggagcag tcagtcgtgt gcaacaagac ttagacagtt atcagcgtcg
    19561 tttgcaggat gcggaagata attacagtag cttaacccat acagttagag gtttgcagag
    19621 tgatgtggta tccccgactg gtaaaatcca atcacgcctt actcaactag caggacaaat
    19681 tgagcagcgg gttactagag atggtgtcat gagtattatt agtggcgctg gagacagcat
    19741 taaattagct atccaaaagg ctggcggcat taatgccaaa atgtctggta atgagattat
    19801 ctcagcaatt aacctcaact cctacggagt aacaatcgca ggtaaacaca tcgctctcga
    19861 tggcaatacg actgtcaacg gtacctttac cacaaagata gcagaggcta tcaagattag
    19921 ggctgatcag attattgcag gcacgattga cgctgctagg attagagtga ttaaccttaa
    19981 cgcaagtagt atcgttggtt tagacgctaa ctttatcaaa gctaaaattg gctatgctat
    20041 cactgatttg cttgagggta aagttattaa ggctcgtaat ggtgcgatga caatagactt
    20101 gcaaagcggt caaatcaacc attacacaaa tgagtcagcc atgagacgta tcgatagcag
    20161 tacagctagt cagtttataa agatgacaaa gtcaggcttt atatctgaga taggtaacat
    20221 gcaagctgca atgacggtta taggctctaa tagcgatggg tcagaaaacc atgaaaataa
    20281 aaccttcgga ggcataagaa tttggaacgg gaagtcatct tatcaatcga caagttttgt
    20341 tgaattagtt ggtaaccgtg tagcaatcta tggaaataaa aatcgtagtc catggctctt
    20401 tgactcaaca acgtcaggat atgcttacct tatccctcaa aatgacagag gtataaagca
    20461 tgtcataggg agagctgatc gtaagataga ccaaatccac gttggagata tctatgtgca
    20521 aggcgaacgt gtagccatga tgttaaaaga tttagcactt aaaattggct atataggtac
    20581 aggtggttgg gctaaccgca taggataaat taggagaaac aatgcaagaa aaattattag
    20641 gtaaaattat taacgattta gcacttaaag ttgctaattt aacgctagaa aatgctcaat
    20701 taaaagcaca gcatgaaatc gaattggaag aactaaacgc acaagatgaa gcaacagcac
    20761 cgaaggaaga aggtaaataa acatgagaaa ttggaaagta acaggaaaat atccacaact
    20821 tgatggcaca ggagcagtcg caagcacaca tattattatc gctgctgagg atggagctgt
    20881 tatcccgcaa cttattaagc aagacttaac ctcaactaat gacacagaga ttatcaaagc
    20941 cgctttggaa gaatttaaaa aatctgaata cgttgaaatt gcaatgggcg aagccgtgca
    21001 aaaagtagac gaccttgaaa aaatctcaca ggaaaccgct aagaccgcta aaacagccca
    21061 aacagccgca ggattagcta aggtgtccgc agagcgtaca cagcgaatga ttaacttgca
    21121 aaccatccac gtattgacaa gtggtgggaa cgttgaaccc gatatctaca aaggtatgtt
    21181 agagcttatt gagcctgcta aacaaggcga gtatcaggct tatgacgtgt ttactgttgt
    21241 agatgagtcg cacgaagatc aagcggtaga agggaacctt gtctttgtac atgtcaacga
    21301 gccgtttact tatgaggcgc aaactcttaa agatttggaa tcagaggata aagtcacagt
    21361 tattaaatat gcggatttgg ttaaacaaga ttagaggtgg ttagatgata gattttgtac
    21421 aaattggtgc tttttgtggc gctgctttgt ctatcttggg tgtttgggga tttatcgtca
    21481 atccctttaa aaaagccatg gaagctaatg agtttgccat ggctcaactc aaggactcaa
    21541 ttaaggagtt agcttatgag cttaaaaatc ttgatcgtga acgtgagatt accaaaaaaa
    21601 ttatcgacag acacgaggag cgtttaggtc gcgtagaaga cgaagttatt atcaacaagg
    21661 agcgtattat tacgctattt aaaaaaggag aagaaaaatg aataactggt ttaaaaaagt
    21721 agcaattaaa acaattaaaa caatggcaca aacggccgtt ggtctaatcg ggtcaagcgt
    21781 gttaatcacg gatattaatt ggccaacaat gttgtcagca gtgctgttgt caggactaac
    21841 atgtatcctg atgaatgtgt cacaaatcaa ggaagaggaa taggtcatgc gagcaatcac
    21901 acgattagca ttagttatag caatcgcaat actgtatgtg ccattatctg tggttgcttt
    21961 gattttttat ccatattgat ggatcgcttg gtgcgcaatg ttgggatggc tacgcagact
    22021 actgtaagta tctaggactg ccatacgcaa actgtacaaa tacaggatat gcaagggata
    22081 tatgggaaca acgtcacaaa aatggtattt taaactactt tgacgaagta gagactatgc
    22141 aagctggaga tgtcgcaatt tttatggtag ttgcaggtgt tacaccgtat agccatgtgg
    22201 ctatttttga tagtgatgca ggtagtggat acggatggtt tttaggtcaa aaccagggtg
    22261 gagcaaacgg agcatataat ttagtaaaaa taccatattc aacgacttat cctactgcat
    22321 ttagaccaaa agtttttaaa aatgcagtta ctgttatagg taatatagga ctaaataaag
    22381 gcgattattt tattgatgta tcagcttatc aacaagcaga cttaaccgct acttgtcagc
    22441 aagctggcac tactaaaacg attatcaaag tatccgagtc actcgcttgg ctgtctgata
    22501 ggcatcagca acaagctaat actagtgacc ctattggtta ttatcacttt ggacgatttg
    22561 gaggagatag caacttagcg caacgagaag cagatttatt tctgtccaat ttaccaacca
    22621 aaaaagtctc ttacttagtc attgattatg aagactctgc aagtgccgac aaagaagcta
    22681 acactaatgc agttattgcg tttatggata aaattgcaaa cgctggatat aagcctgttt
    22741 attacagcta taaaccattt acgcttaata atattgatta tcagcaaatt atcgctaagt
    22801 acccaaacag catttggata gctggttatc cagactacga agtacgaaca gagccacttt
    22861 gggagttctt cccttcaatg gatggtgtgc gctggtggca gttcacaagt gtaggagtag
    22921 caggtggttt agataaaaat attgtattat tagcagatga tagtagcaaa gtggatatac
    22981 ctaagattga caaaccacaa agccagctta cttttaatca aaagctagat actaacacta
    23041 aattagacaa ctcgaatgta ccttactacg aagcaaccct tagaacagac tattatgtag
    23101 agtctaagcc aaacgcaagt agcgctgata aagaatttat caaggcagga actcgtgtaa
    23161 gagtctatga aaaagtgaat ggatggtcac gtattaacgc ttctcagtct gaccagtggg
    23221 tcgaagataa gtatttatct aatgcaacac aagtataaaa taggaggtaa agctccttta
    23281 gataagacaa atgccctcgc ttttgcgggg gctgtttttg ttataaaga
//
LOCUS       DAUGHY010000027        23199 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.027, whole genome shotgun sequence.
ACCESSION   DAUGHY010000027 DAUGHY010000000
VERSION     DAUGHY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23199
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.027"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            476..655
                     /locus_tag="VH752_001065"
     CDS             476..655
                     /locus_tag="VH752_001065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ8289427.1"
                     /translation="MTKLERPQEENEYLRTEVAYLKKPRELRLKEASLQRRRQKTIIK
                     NGQRRALIRFSFKDG"
     gene            complement(804..1727)
                     /locus_tag="VH752_001066"
     CDS             complement(804..1727)
                     /locus_tag="VH752_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289428.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1720..2790)
                     /locus_tag="VH752_001067"
     CDS             complement(1720..2790)
                     /locus_tag="VH752_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289429.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGRQITEVIIKHQKVSNAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQDLHQKILRKMSQQEEGNV"
     gene            complement(2799..3725)
                     /locus_tag="VH752_001068"
     CDS             complement(2799..3725)
                     /locus_tag="VH752_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289430.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGIGLPTTTPSLGRLIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3725..5227)
                     /locus_tag="VH752_001069"
     CDS             complement(3725..5227)
                     /locus_tag="VH752_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289431.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSLIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAIYKKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDRFSTATMTFMLALPSIAVI
                     YVVRFLGGMVGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5292..7262)
                     /locus_tag="VH752_001070"
     CDS             complement(5292..7262)
                     /locus_tag="VH752_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ8289432.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTIGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKAEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDNAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVT
                     AKQYENAKEKWMKAKAKSNAKYAEKLADHVEK"
     gene            7593..8825
                     /locus_tag="VH752_001071"
     CDS             7593..8825
                     /locus_tag="VH752_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ8289433.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDAKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFAGQTI
                     YSYNYMLKGMPCYREGVDGLFVGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            8993..10174
                     /locus_tag="VH752_001072"
     CDS             8993..10174
                     /locus_tag="VH752_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ8289434.1"
                     /translation="MTKRIILTIFTFICFSMMPLVHAEDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRINA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYTFLRKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSPKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFAMGR
                     Q"
     gene            complement(10326..11744)
                     /gene="sufB"
                     /locus_tag="VH752_001073"
     CDS             complement(10326..11744)
                     /gene="sufB"
                     /locus_tag="VH752_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEQ8289435.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(11737..12216)
                     /locus_tag="VH752_001074"
     CDS             complement(11737..12216)
                     /locus_tag="VH752_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEQ8289436.1"
                     /translation="MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVQLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV"
     gene            complement(12203..13429)
                     /locus_tag="VH752_001075"
     CDS             complement(12203..13429)
                     /locus_tag="VH752_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ8289437.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATHKYEASRQQVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLANKLTT
                     KTRFVSLVHVSNVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMINFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQPSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(13460..14722)
                     /gene="sufD"
                     /locus_tag="VH752_001076"
     CDS             complement(13460..14722)
                     /gene="sufD"
                     /locus_tag="VH752_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEQ8289438.1"
                     /translation="MTKEKLVAFSQAHAEPAWLQERRLAALEAIPELELPTIERVKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGVVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKISANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWALAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKGAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLNQETAERLVIRGF
                     LGAVIAEIPIPSVHQEIIKVLDEKLLNR"
     gene            complement(14817..15587)
                     /gene="sufC"
                     /locus_tag="VH752_001077"
     CDS             complement(14817..15587)
                     /gene="sufC"
                     /locus_tag="VH752_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEQ8289439.1"
                     /translation="MSILEINNLHVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAAIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADEDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGKDFGAMIITHYQRLLNYITPDLVHV
                     MMDGRIVLSGDAALATRLEKEGYAGIAQDLGIEYKEES"
     gene            complement(15709..16878)
                     /locus_tag="VH752_001078"
     CDS             complement(15709..16878)
                     /locus_tag="VH752_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEQ8289440.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARRV
                     NKVPMPTSGGLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVITLTGF
                     LDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFLTVLWI
                     VSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLISAIAGFF
                     PYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDTIVAII
                     RRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSSRIGGV
                     LLMLGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDLKH"
     gene            complement(16885..17646)
                     /gene="mecA"
                     /locus_tag="VH752_001079"
     CDS             complement(16885..17646)
                     /gene="mecA"
                     /locus_tag="VH752_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEQ8289441.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAADPSTNHESEPLDYVH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            complement(17792..18631)
                     /locus_tag="VH752_001080"
     CDS             complement(17792..18631)
                     /locus_tag="VH752_001080"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEQ8289442.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIAFIWIEKQNAQQEPAVTDLARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTHYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            complement(18697..20622)
                     /locus_tag="VH752_001081"
     CDS             complement(18697..20622)
                     /locus_tag="VH752_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEQ8289443.1"
                     /translation="MKKILMTLVLCFSLLGIRIKAADVDYSITNYEGQLLLSKENTAR
                     FEQKVTYQFDTSYNGQYISLGRTGHLPAGFAIDQKPKVEVYQNGQQVPVSQEFSDLGD
                     GYRLKLYNAGQAGDKVDVKVIWQLHHLLTAYQDVAELNWTPISDWDKTLEKVSLTVTT
                     PTDIQDSNLWAHRGYYQKKPQVLKEGNSRYQINAKNVSGQLELHAYWDKKALLGKEPV
                     DVSTSKKNKIVALETKISRRRTLLQLLFGKVIPLVEVGFLLSQLIQFTRLKKQFNRYH
                     LANHTDHSYEVPEDLSPLVLTQAIYGQSFAYLSPTASESQKLLIPKGVTFEALVQATL
                     LDLIDQKVLLLTKEEGKAYLEISQLDRVTDEEAAFLDMAFGNKVTLPVDQLFSQYHYD
                     ADTIKQLKKTYKGKKLEQEVRQSSEQVIKAMKKASATITNNVLETIKKLNLPDTYRQM
                     TPAEKRKSHSVQGLGCLLVVLNSSLLIYLTMKGSGLALIYLALMVLMMCLGFYISLKL
                     DQYKKLGIETPEGGVRLHQWQSFKNMIRDIDKFEDVAIEGLVVWNRVLVYATLFGYAK
                     KVERYLKVHRIALPEVYQAVRPGELSMVMYATTPTFVSSLSSATTSSNFSVSSGGGIS
                     GGGGFSGGGGGGGGGAF"
     gene            20820..22388
                     /locus_tag="VH752_001082"
     CDS             20820..22388
                     /locus_tag="VH752_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ8289444.1"
                     /translation="MKKLILSCLVALALLFGGMSRAQANQYLRVGMEAAYAPFNWTQD
                     DASNGAVPIEGTSQYANGYDVQVAKKVAKAMNKELLVVKTSWTGLIPALTSGKIDMIA
                     AGMSPTKERRNEISFSNSYYTSQPVLVVTANGKYAEAKSLKDFSGAKVTAQQGVWHVN
                     LLTQLKGAKLQTPMGDFSQMRQALTSGVIDAYISERPEAMTAEAADSRLKMITLKKGF
                     AVAESDAAIAVGMKKNDDRMATVNQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAKAS
                     FLGQMWAIFKGNWKQFLRGTGMTLLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALG
                     QKLFGWLLTIYIEIFRGTPMIVQSMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFVLTFTVTRILRHIERRFDADTYT
                     TGANQMQIAEVSNV"
     gene            22381..23121
                     /locus_tag="VH752_001083"
     CDS             22381..23121
                     /locus_tag="VH752_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289445.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRAQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDRIIFMDKGL
                     IAEEGSPQQIFENPTQDRTKEFLQRFLK"
BASE COUNT         7190 a         4823 c         4180 g         7006 t
ORIGIN      
        1 ttcatctttt ttcaataagt gtgttacagg aacctctctg acagctttat tagtatacga
       61 tatctttact gtcattgtca actctttttt tacttttttt gtactttttt atttgcccgc
      121 gttgtaaaat gaagtgcaac aaaaaagagg aaaagctttg aactacaccc caaacgttag
      181 acaaaaaaat ctaacgttcg gggtattttt tatgaaatta agtgatgaag gtacgaaata
      241 gacatcgtta agaaagaaaa aaatacctac tatccaccag agataaagca agaaataata
      301 gataacgttt tgatagaagg cccgccacaa taccaagtat cacttgttta tggcttgtta
      361 catgcaggaa ctctcccaag ttgaatagca caatataaga aaaacagtta tactgttgag
      421 aaaacaagag caagaccatc acaaacagga cgtaaaccaa aagaaacctg aagaaatgac
      481 taaattagag cggcctcaag aagaaaatga atatctcaga actgaggttg cttacttaaa
      541 aaaaccgagg gaactccgtt tgaaagaagc atctctgcaa agaagaagac aaaaaaccat
      601 tataaagaat ggacaaagac gagctctgat tagattttct tttaaggacg gctaaattga
      661 ctcgttctac ctactattat catcgattat tgcaacaata aacgtatcaa aataaaacga
      721 aaaggactta gtcctctaca acacagaact aaatccttta cttcaaatag agtagccaac
      781 ttttggggct cagtgcatct cttttacaat tctttttgat atttttcaat ttctgcttgg
      841 tttgcccaaa caaagtgatt tggtttgatt tcaaccattg atggcttatc taatgtataa
      901 tcatgctggt ctggatgata gacaacaagt tctttttgac gctctaaaat tggatctggg
      961 ataggcacgg ctgataacaa agattgggtg tagggatgaa ttgggttatt aaacagttct
     1021 tctgtttctg caacttctac aataacccct ttatggataa ccgcaatacg atctgaaata
     1081 aagcgaacga ctgaaagatc atgggcaatg aagagataag tcaaaccttt ttcggcttgc
     1141 attcttttaa gaagattcaa gacctgtgcg cgaacggaaa cgtccaaagc tgaaatcggt
     1201 tcatcagcaa taacgaattc agggttcatt actagggctc tagcgatacc gattcgctga
     1261 cgttgacctc ctgaaaattc atgagggtag cgcgtcaaat gctctgatag caaaccaact
     1321 tcggccatca tgttcttaat tttttcttta cgttcttcct ctgttttaaa cagattaaag
     1381 ttataaaggc cttctgaaat gatgtagtca acggttgccc gttcattcaa actagcagcg
     1441 ggatcttgga aaatcatttg aattttacga atgagctcat tggcttctga ttttgatttt
     1501 ctgccattaa ttactttccc atcgtataaa atttgacctg aactagtatc gttcaaacca
     1561 ataatagcac gaccaattgt tgttttacca ctcccagact ctccaactaa agaaaaggtt
     1621 tctccttttt taataaagaa attagcattt ttaactgcaa caaatttttt ctttccttct
     1681 ccgaaggaaa tttctaggtc ttttacttcg actaatttct cagacatttc cttcctcctg
     1741 ttgtgacatt ttccttaaga ttttttgatg caaatcttga atgacttctg gtttttgaac
     1801 ttttggagcc tctgggtgta aaagccatgt tttggcccaa tgagtctcag atacgttaat
     1861 cgccggaggt gctttttcaa agtctaaaac catagcatat tctgagcgaa gtgcaaaggc
     1921 atctccgata attggtgaat aaagtgatgg aggcgttcct ggaatagcgt aaagttcacc
     1981 agattcatct gccaactgcg gtaagctaga cagcaaactc catgtatagg ggtgtcttgg
     2041 atcatagaaa atctcttcga ctgttccaaa ttcaacaatt tctcctgcat acatgacagc
     2101 cactttatct gcaatgcttg ccacaacacc taaatcgtgc gtaataaaga taatggtgaa
     2161 atgatattct cgttgaagcg acttcaataa ctcaacgatt tgagcttgaa tagtcacatc
     2221 aagggctgtt gttggctcat cacagataag aatatctggg cgacaagcta aagcaatagc
     2281 gataacaata cgttgacgca ttcctcctga atactcaaat gggtaatctt caaagcgttt
     2341 tttggcattt gggataccca ctttattcat gtaatcaagg gccatttctt tagctttggc
     2401 attacttact ttttggtgct taataatcac ttctgtgatt tgtctaccga tagttttaat
     2461 gggactaaga ctggtcattg ggtcttggaa aatcgttgcg atttttgagc cgcgaatctt
     2521 tgcccactct ttatttgttt ttaaatctgt caattcttgc ccacgataga caattgaacc
     2581 attagcaatg cgtccattag actccaacat ccctgtaaag gtctttgtta aaacagattt
     2641 acctgagcct gattccccta caaaagcaag gacttctcct tcaacaagtt ccagtgagac
     2701 gttacggata gctgttaaaa cacgatcacg cacatcaaat tctaccacca catctttggc
     2761 agttaagatt acattatttt cttttgtcat tttaactcct atctatgtga acgtgggtca
     2821 ctggcatcag ccaagttttg tccgacaatg tatagtggta acgatactaa aatcaatgtt
     2881 actaagggaa tccaaaagag gtaggcattt gttgttaagt tgcttgaata attagcaatc
     2941 aaacgtccta aacttggagt ggtggttggt aaaccaatcc caaagaagga taagaaggcc
     3001 tcagaagata cataaactgg tagcatttgt gacaacatag tcatgataac tgaaaccaat
     3061 tgaggcagga ggttcttaac agcaatcttg tacatgggtg ttcccaaagt ttgactagca
     3121 aggttgtatt ctaaatcacg gtaacgcaag atttgaacac ggatggagta ggcgacacca
     3181 atccatccag tgatacagaa agctagaatc aaattccaaa atcctgcacc taatgaatag
     3241 gtcaaaacaa tgataataag catagaaggg atatttgaga taatgttata aatttcaatc
     3301 ataactttat caaatgcttt agaaactccc catatggctc ctaacactac cccaatggtg
     3361 atattaatta gtgtcgctat aactgagatt aaaatagaat tacgtgcccc ataccaaaca
     3421 ccatcaaaca gagattgccc atttttgtcg gttccaaacc agtactctgc atttggccaa
     3481 atataacgct ttgaaaagtc attgatatta ctaacgtcat taaagtcgta gttggcaaac
     3541 attggataaa taaagctcat catcaagact gtcactaaaa tcacgagcat aaagactgta
     3601 gattttttag aaaaaaactg acgaaacact gatttccagt aagaataagc aggggtatca
     3661 atcacttcgg aggcttcact atcgcgctca acaaatcgaa atttagattt atcaatcgat
     3721 tccattactt accccctttt tttgttgaca atttaatacg tggatcgaca agggtcatga
     3781 caatatctcc tagtaaaagt gaaacaattg acaagacagt gaagataaag gttaatccaa
     3841 caatcataga attgttagca gatttaatag aatcaattaa cattttaccc atacccggga
     3901 aggcaaagac agtctctgtt aaggttgcac caccaatagc aaggataata cttgcaggaa
     3961 cccctgacac aattgggacc atggcatttt tgaagagatg ccctctgtaa atttcagatt
     4021 ctgataatcc ttttgaacgc gcaaaacgta cccaatcgct ggcctgaagg tcaacaagat
     4081 agcgacggaa ccagataacc gtagttggaa tatttaaaat tcctaaaata agggctggta
     4141 aaatataaga tttaggatca gatgccccca gcattgggaa actatctggt aaaccaacca
     4201 tgccacctag gaagcgaaca acatagataa cagcaatact tggtaaagca agcatgaagg
     4261 tcatggtcgc tgttgagaaa cggtcaaaat aggtattttt aaaccgagcc ataaataaac
     4321 caagaggcaa accaacgata taggaaaaca taacaccaaa aaaaccaatt acaaaagaat
     4381 tgtgaatcat tgatgggtcg acataattat tgatggtttt agtgtaggaa tctcccttac
     4441 catagtttac cttggtgata tcatccaatg attttggatt tttgtagcta cggctataga
     4501 tatccactga cgatttctta gtgacacctg atgggaaggt cacgtcttga agagctgttc
     4561 gtccttgccc ttgtgaaata acctgaagaa caggaatatt gctgtatgtt ggataagatg
     4621 ttcctaagtt tagggtaaca aagttttggt gaagataagg aaattttccg ttagtataga
     4681 ggagatattt atgttttgtc cccgaaccaa caagtgacca gccaactgat ttatcttttt
     4741 ctagacgaat atacctagct aattttggat tatctttatc ctgaatcttc caagggtgat
     4801 caataacaac tagatttgag aaaaagttcc aaacacgttc gtaaatagga atattgcgaa
     4861 ttgcataaaa ctgtttgcta gtggggaagc gttttagctg ccagccattg ccaagagaat
     4921 caatatactt cttatagata gctttgttag cactagttgc ttctgtcgtt acactactat
     4981 ctactttaga agcattatct tttaattctt tgcttgaaaa ataattgaca taacccatac
     5041 gttgaaaagt tagattttca tacgctactt ttttatctgg tgttgttgtc attttattat
     5101 aattagggtc ttgtttaaag ataaggctgg taggtactag agtatatacg atggtgtatg
     5161 ttaaagccgt taccaaaact accgacacta acgatcgcat gatacggttt aaaatgtatt
     5221 ttttcatagt tatcctcaat agttcaaaag aactccctca cataaacgag aaagttcttt
     5281 tgaacatcgt tttatttttc aacgtgatca gctaacttct cagcatattt tgcatttgac
     5341 ttagcctttg ctttcatcca tttttctttt gcattttcgt attgttttgc tgtgacaggt
     5401 ttgtcttgaa gtttcattcc tttatatgct agaggacctt tagaccctgc ccaagagaag
     5461 cccccgctaa atggaacggc tttagtaact cgtggagtgc cacccaatgc cacaactgga
     5521 atatctacgg cattatctgt aaggtaggct tgtgcttttg cgtaagcttt atagcgcgca
     5581 tcgttgtcgt ctgtaattgc tgctgcttca tcaagaagag tttggtaggt atcaagacct
     5641 gcagctgcca caacatcctt attttgacct gctttgattc caagtttttg gataacagat
     5701 ccaccaccta ctggactcat gatgtcaagg taggtccgtg gatcttggta gtctggtccc
     5761 caccatgatg aaatgatatc gtagtcttgt tgttctgggg tctcagcata gaagccttgg
     5821 gcttcgtgag ttgatgtttc tgtttcaaga acattgacaa tgacattttc tttaccaaga
     5881 gatgcttcaa cagattgttt gaaagactga gcttcctgaa cagttgctgc gtttgcttgg
     5941 tcaacagggt aatctaattg aactgggaag gttacacctt cagctgtcaa agcttctttg
     6001 gcttttgcaa actcagcttt tgctttttca ggattataga aaccatcttg agcatcagct
     6061 aagttaacgt ctttccattc atcaccaagt tttgccattt ccttttcaac ttctgaacca
     6121 aaatcacttt ctccaatggt cacaaatgtt ggtggcacaa gcatgttacg taaggctttt
     6181 gttttggcat cttgacctgc agtttgtgct tggaatgacg ctcggtcaaa agcaaactga
     6241 atagcttgac gaaaatcctt gttgttaaga gctttcttac cggcatcttg ttgtgcaggg
     6301 tctttcttag tgtttttgaa agaagtgcgg ttcaaattcc atgttaaatg acggatatct
     6361 ccagtcaaca ttccgtaagt aatgttatca gcatagtttt tcttagctga tttgtaggta
     6421 gggtcatttg ggtaaagtcg tgcaacgctg aactcaccct tgtcaaagtt cttgtagaac
     6481 gaacctgggt ctgaaccatc tgagtaagtc aatttaacag attctatccc aacattctta
     6541 gcatcccagt agttttcatt tttatggaat tccatagatg attttgaggt gaaggcgctc
     6601 aagaagtaag caccattaac aaggattgat gatggatcgg ttgtaccaaa atctttacct
     6661 tttgacttca aaaatttcgc attaactggg aaaagcacac tataagttgt ttttgaattc
     6721 cagtagcttt caggcttgtt caaagtatac tgaacagttt tatcgtcaag ggctttgaca
     6781 ccaacttctt taaaatctac ttcaccattt tggtaagcct ttaagttttt tattgaatct
     6841 tcaacaacgt aaagagcatc tgatttatcg tcaaccgcgt gcttcaaacc agtcacaaaa
     6901 tcttctgctg ttactggggc atattcttca ccatcagccg tataccaaga gacaccatcg
     6961 cgaagagtat aagtataagt cagaccgtct tttgaaacct tccaatcttt agcaagtgat
     7021 ggtactaaat taccatactc atcgttttcc aaaagaccat caaccatttg tgttatcaca
     7081 tcagtcgttc caccgccatt agtcaaaata taatccaatg attttggatc gttaacaaaa
     7141 acgtacttgt aggtttttgt agcttctgag ccacctgaag catttttatt accacaagct
     7201 gccaaagcgg atactgacaa gatcgcaaca cttacagctg ccaaccattt acttttcttc
     7261 ataaaagtca cccctaaact ttatttttca ttttttggca attgctaaat gaaattggtt
     7321 aattgaagac attatatcat aaaactttct taaagaaaat aatgaaatga aaaattatct
     7381 gaatatttcg tagaattttc tttattttat acatttatct atatgaaagc gttacactcc
     7441 ttttttaatg aggtttcaat tagtttaata aagattgtca ttctctcatg gtagcgtttt
     7501 attacatcac tactcttgtg aggtaagtca ttaccttaga caaaaatatg gtataattaa
     7561 aaaaataata cttgagtaag gataatctat aaatgatcaa acgattaatt tccctagtgg
     7621 tcatcgcctt attttttgca gcaagcactg ttagcggtga agagtattcg gtaactgcta
     7681 agcatgcgat tgccgttgac cttgaaagtg gcaaagtttt atacgaaaaa gatgctaaag
     7741 aggttgtccc agtcgcctca gtcagtaagc tcttgacaac ctatctggtt tacaaagaag
     7801 tttctaaggg caagctaaat tgggatagtc ctgtaactat ttctaactac ccttatgaac
     7861 tcactacaaa ctatactatt agtaacgttc ctcttgataa gagaaaatat accgttaaag
     7921 aacttttaag tgcgttagtt gttaataacg ccaatagccc cgctattgct ttagctgaaa
     7981 aaataggcgg aaccgaaccc aaatttgttg acaaaatgaa aaaacaatta agacaatggg
     8041 gcatttccga tgcaaaggtc gtcaattcaa ctggcttaac taaccatttt ttaggagcta
     8101 atacttatcc taatacagaa ccagatgatg aaaattgttt ttgcgccact gatttagcta
     8161 ttattgccag gcatctctta ttagaatttc cagaagtact gaaattatct agcaaatcct
     8221 ccactatttt tgctggacaa accatttaca gttataatta catgcttaaa ggcatgcctt
     8281 gttatcgaga aggcgtggat ggtctttttg ttggttattc taaaaaagcc ggtgcttctt
     8341 ttgtagctac tagtgtcgaa aatcaaatga gggttattac agtagtttta aatgctgatc
     8401 aaagccacga ggatgattta gctatattta aaacaaccaa tcaattgttg cagtaccttt
     8461 taattaattt tcaaaaagtc cagttaattg aaaataataa accagtaaaa acgttatctg
     8521 tcttagacag tcctgaaaaa actgtcaaac ttgtagccca aaatagttta ttttttatca
     8581 aaccaataca tacaaagacc aaaaataccg tccatattac taagaaatca tccacaatga
     8641 tcgcacctct atcaaaggga caagtcttag gtagagcaac ccttcaagat aaacatctta
     8701 ttggacaagg ttatctggat actcctcctt ctatcaatct tatccttcaa aaaaacattt
     8761 ctaaaagttt ctttttaaag gtctggtgga accgttttgt gaggtatgtc aatacctctt
     8821 tatagaataa cacaatgctt tttgtatgac attgactgac agagtacttc tgcagtcttt
     8881 ttcattttta tttaagcaaa ctaggctaat cctaacaatt tttcaaaaaa acttcacatt
     8941 gtattagcaa agtgatatac tgaaaatgca ttacatttaa aggagtcgga aattgaccaa
     9001 aagaattata ctaactattt ttacctttat ttgcttttcg atgatgcctc ttgtccacgc
     9061 cgaagatgtc atggatatta caaggcaagc aggctacaca gttagtgagg ttaatcgacc
     9121 aaaatcttct attgtcgttg atgcaaatag ttcagatatt ctttggcaag ataatattga
     9181 tattcctagg gatcctgcta gtatgtctaa gatgtttacg ctttacattt tatttgaaga
     9241 gctcgctaag ggaaaaatta cgatggacac tactattacg gcaactccaa ccgatcaagc
     9301 tatcgctaac atttatgaga ttagcaataa taatatcgtc gctggagtag cttatcctat
     9361 tcgtgatctt attactatga cagctgtgcc ttcatcaaat gcagcaactg ttatgattgc
     9421 caattattta tcaaacaatg atgcttctgc gtttatcgat cgtattaatg ccacagccaa
     9481 acaattaggc atgactaaca cccatttttc aaacgctagt ggtgcagcag cacaagcttt
     9541 tcaaggttat tataatccta ctaaatatga tttatctgct tcaaatatta cgactgctcg
     9601 agatttgtcc aagttactct ataccttctt aagaaaatac cctgaaatta tctcctttac
     9661 aaataaatct gttgtgcaca ctatggtagg aacgccatat gaagaagaat ttcacaccta
     9721 taaccactct ctcccagata atcaatttgg tatgaaagga gttgatggat taaaaacagg
     9781 ttctagtccc agcgctgctt ttaacgccat gataactgcc aaaagaggca aaactcgact
     9841 cattactatt gtcatggggg tcggagattg gtcagatcaa aacggagagt tttatagaca
     9901 tcctttcgtt aatgccttga ctgagaaagg gtttaaagat agtaagacct tatccaaaaa
     9961 agcacgccaa aaactcgaaa aattagtgcc acaaaccaaa aaagaaacat cacctaaaca
    10021 gcagcacttc aaagccacta aaaagcaatc gtacttggaa agagtcgaag attttatgaa
    10081 tcacaaccat actttcttgc ttatttgctt agctatcttt ataattacta tccttttact
    10141 aagcctcgtt gtctttgcaa tgggacgcca ataatgaatt atcaaaaacc tctgtcaaag
    10201 cacagaggtt tttgatatgt tatcttacgt gtttaaggca actaaaaact aaccacttgg
    10261 ggttagtact acaactcata aaattctatc tcaaagatct ttcagatgga taagagaaaa
    10321 tgcaattaac cgacagaacc ttccatctca taggaaatca atcgattgag ttcgaccgcg
    10381 tattccattg gcaattcttt ggtaaaaggc tccacaaatc ccatgacaat catctctgtg
    10441 gcttcgcttt ctgataagcc acggctcatg agatagtaga gttgttcttc agaaatctta
    10501 gacactttgg cttcatgttc taacgccacc tgtgaattat gaatttcgtt aaatgggatc
    10561 gtatctgatt tagaaatatc gtccatcaaa atagtatcac attcaatatg ggaaacggat
    10621 tttttagatt gcttgttaaa ggtcacttgg ccacgataat ctactttacc accagacttg
    10681 gcaattgatt ttgacacaat tgatgatgat gtatgtggag cattgtgaat cattttagcc
    10741 cctgtatctt ggtgttggcc tgcgttagca aaggcaatag acagcatggt gccacgcgca
    10801 cctggcccat caagataaac agaagggtac ttcatggtgg ttttagctcc tagattgcca
    10861 tcaatccatt ccactgttgc atccgtaagg gcacgtgcac gttttgtcac tagattatac
    10921 acattgtctg accagttttg aatggtggta taacgcatgt atgcaccgtc aagcgcaaag
    10981 atttcaacga tagcagcatg taagctgtta cttgaataag tgggagctgt acatccttca
    11041 acataatgaa cacttgctcc ttcatcaaca ataatcaatg tccgctcaaa ctgaccagta
    11101 ttttcattgt taatgcggaa ataagtttgc aaagggatat ctaccttgac ccctttagga
    11161 acataaataa aggtaccacc agaccaaact gctgaattga gggctgctaa tttgttgtct
    11221 gttggcggaa ccagtttggc aaagtattgt ttgaacaaat caggatattc tttgagggcg
    11281 gaatcggtat ctgtaaagat aatccctagt ttttcaaatt cacccttcat gttatggtaa
    11341 accacttctg actcatactg agctgatgct ccagcaagat aagcacgttc tgcttctgga
    11401 attccaatac gatcaaaggt ttcttttatt ttttctggaa catcatccca agaacgagct
    11461 ggcttatcag atgctttttg atagtaaatg atatcatcaa agttaatatc tgataagtct
    11521 gctccccagg tttgcatcgg cattttatta aaggtttcca aggattttaa acgaaactcc
    11581 aacatccact caggttcatt tttggcagct gatagttcgc gaaccactgc ctcactcaat
    11641 ccttttcctg tagaataaat gggctctaca tcatcgtgaa atccaaattg gtagtcccct
    11701 aagtcaattg gctttggctc tactttctca tttatatcag acatttttcc cttcctttac
    11761 attttggtcc gtgaggtgct gagcattggc acttcgttta atagcttcct tgagagcatt
    11821 ccaagctagg gtagagcatt tgatacgctg tggaaatttt gcaactcctg ccaacaattc
    11881 tgcttcacct agctcttttt gagcaggatt ttcctgtcct tgtaccatct ctgaaaaaat
    11941 atcagctaac gcgagtgctt cttctttact tttaccaata acagcatctg tcatcatgct
    12001 tgatgaagct gtggaaatgg tacagccgtt gcctgcaaaa gcaatatctt caattttatc
    12061 ttcgtcaaac ttaacggtca aagaaatcac atcaccacaa gtcggattat tcagttgaac
    12121 agcctctacg ccatctagtt gcccatgatg atgtggacgt ttcgaatggt ccgctaccac
    12181 agccatgtat agatggttca gtttagagag tgccattgaa aaactccttt gcttttagaa
    12241 ttgcttcgac taaacggtca caatctgcct tggtgttata gatataaaaa cttgctctaa
    12301 cagttgctgg tacacctaaa taactaagaa gaggttgggc gcagtggtgc cctgctctta
    12361 ctgcaacacc ttcatagtcc aaggctgttg ccaagtcatg aggatgcaaa tcatccagat
    12421 taaaagaaat cagaccagat cttgcactag gctggcttgg tccatatatg gtaagcccct
    12481 caatagcttc taatttcggc aaaacatagg ctattagttc tgcttcatgc gcatgtatat
    12541 cagccatgcc tagtctctga aggtaagaaa tggctgcgct tagcccaata gcaccagcta
    12601 tgtgaggtgt tcctgcttca aacttccagg gcaattcttt ccaagtggcc tcttgttcgt
    12661 aaacaaaatt aatcatttct ccgccaaatt caagaggctc cacttgattc aaaagctctt
    12721 ctttgccgta aagaacaccc aaacctgttg gccccaacat cttatgagct gaaaaagcaa
    12781 agaaatcaca atccaagtct tgaacatcaa tagccaaatg tggaaccgac tgggcaccgt
    12841 caacaacaag gtaggctcct ttagcatgtg ccagcttggc aatttcttta atgggattga
    12901 tgcaaccaag aacattggag acatgtacta ggctaacaaa acgtgttttt gtcgtcaatt
    12961 tgtttgccaa atcgtccatg tcaagttggc catcttttaa ataaacatag actaaccttg
    13021 ctcctgtttt ttgacaggct tgttgccaag gaatgatatt ggcatgatgc tccataatcg
    13081 aaatcaacac ctcatcttct ggcgtcaaga cctgctctgc aaaccgagca acccaattca
    13141 aactggttgt tgttcctctg gtaaagagca cttccttgct tgatttagca tgaataaagt
    13201 cagcaacctg ctggcgactg gcctcatatt tgtgcgttgc acgttcagcc aaggtatgaa
    13261 ctcctcggtg gacattagca ttatcttctt gataatagga ttgcaaagcc tcaagaacaa
    13321 gcgccggttt ttgtgtggtg gcggcattat ccaaataaac aaggggttca tcattgactt
    13381 gttgatttaa gatttgaaag tcttgtttga tgtcttttgc gtctagcaat actaacctct
    13441 ttctttttgg cagtaggtct taacgattaa gcaatttctc atctaaaacc ttaataatct
    13501 cttggtggac tgatggaata ggaatttcag cgataaccgc tcctaggaat cctctaataa
    13561 ccaatcgttc tgctgtttct tgatttagtc ctcgactcat caagtaatac atatcttcag
    13621 ggtcaacctg accgatagaa gctgcatgac ctgctgttac ttcattttca tcaattaaga
    13681 ggattggatt ggcatcggct cttgcttggt cagaaagcat caaaacacgg ctttcttgtt
    13741 gagcatcagc tcccttagcg ccttttagaa tatgaccaat cccgttaaac gttaaggtgc
    13801 cacgttccaa aatcacacca tgctgtaaaa tatgaccaac cgtacgttga ccatagttgg
    13861 tcacgcgcgt gtcaatacct tgtacctgac gaccacttga ggctgcaaca actttcaaat
    13921 cagcttgtga gccctgacca atcaaatcac tgtcaaaatc agcaatgaca ttgccttcat
    13981 tcatcacagc taaggcccaa tcaatgttgg catccttctc taaacgtcct cgacggctaa
    14041 tataggttgt cactgaagga cctaagcggt cgatagccga gaatttaatc tggctgcctg
    14101 cttgagcaat cacttctaca ctgatattag cgctgatctt ttgagtggca ttgccaatag
    14161 attcaaaacg ctctaaatag gtgaacttac tttcttttcc tgcaatcact agaacatgct
    14221 tgttaaaagg aacgtcactg tcactatctt gtaagaaaat agcttcaata ggagttgtga
    14281 tttccaagtg atcaggaacg tagagcacgg ctgcgctatt aaaataagca gtgtggtagg
    14341 cagctagttt gtcttcatca aaagctaatg cctgaccaaa atgagcttca attacttctg
    14401 ggatttcctc aagcgccgta taaaaatcac tgaaaacaac tcccttgtca attaacgcca
    14461 ttggtaactg ttctaagact gtttgcgtgc ctacctgaac aagctttggg ttatctccaa
    14521 tagctataaa atctggaaca ctagctagac tttcattttc tgttaaggta ccatctccta
    14581 gattccaacg gtgaaattta accctttcga tggttggtaa ctctaattct ggtatggctt
    14641 ctaatgccgc taaacgccgc tcttgcagcc aagcaggctc agcgtgggct tgcgaaaaag
    14701 ccactagttt ttcttttgtc atctgtcaat cctttgtctc tggtttttaa attggctggg
    14761 tatatagaac atcacctagc tttttgtaga aagctaaagt aattgtctcc aaaaacttaa
    14821 ctttcttcct tatactcgat tcccagatcc tgtgcaatac ctgcgtagcc ttctttttca
    14881 agacgagtag ccaaggcagc atcaccagat aagacaatac gaccatccat cataacatga
    14941 actaggtctg gggtaatgta attcaacaaa cgttgatagt gggtgataat catagctcca
    15001 aagtctttcc cacgcatctc attaacccct ttagagacta cttttagggc atcaatatca
    15061 agaccagaat caatttcatc taacaaagca aatttaggct ctaacatgag caattgaaga
    15121 atttcattgc gttttttctc cccaccagaa aaaccttcat tgaggtaacg ctctgccatc
    15181 tcttctttca tacctaacaa agccattttc tcatcaagtt tggtgataaa atctcgcacg
    15241 gaaatcttat cctcatctgc cttaccggca ttcattgctg cacgcataaa ttcagcgttg
    15301 gtgatccctg gaatttctga agggtattgc atggcgagaa aaagtcccag tctcgcacgc
    15361 tcatcaactt ctagatcgag aatattgacc ccgtcaagca aaatttgccc ttgggtcact
    15421 tcgtagttag gatttcccat aatagctgct gaaagggtag actttcctgt cccatttggc
    15481 cccataatgg cagctacttc cccagtttta agagttaggt taaccccttt caaaatctct
    15541 ttaccttcta ttgaaacatg taaattatta atctcaagaa ttgacatgat acacccctta
    15601 ttttctctgt catctatacc atctcaagaa aagacagtgt catttgcact gcttttttta
    15661 taatggtcac tgcatctatt atagcaaaaa aagccttact cggctttttt agtgtttcaa
    15721 atccttcttt tctttccatt tcagaagcat tgcttgacga taatcactat taccaataaa
    15781 ctttaacaag ttaaacaaag gcgttcgctt ttcaccccag atctccaatc cttcaataaa
    15841 aacttctaaa ccaaataaaa gtccaagcat caagaggacg ccaccaattc gactagaaac
    15901 atttaaaagt aaagaaataa gggaaaaaag catggtaata ccataaacta ctaagacagc
    15961 tcctcgatga gtaaagccca ttgataagag tcgatgatgc aggtgcatct tatccggctc
    16021 gtagaatttt tgacctgata aactacgtcg aataatagcc acgatggtat ccataatggg
    16081 tacaccaaga ataataacag gtgtcaccac agccacagcc gttgaatttt tcaatccttg
    16141 aagagacaaa acccctatca taaagccaat aaagagggct cctgtgtcac ccaaataaat
    16201 catagctgga tggtaattat aaggaaaaaa tcctgcaata gcagaaatca agaccaaaat
    16261 ggttaacgtc aaaaagaaat ccttttgagg taaaaagaaa taagatacaa tagccatggt
    16321 cactaaacta ataatagaga ccccactaac caaaccatcc aaaccgtcaa tcaagttaat
    16381 agcattagtg atggaaacaa tccacaggac tgttaagaat aaggttaaga aaggaccaaa
    16441 aactaacaac ggccctccaa aaggaatttt gaagctatca aatttaaagt cggtaaaagc
    16501 ccagacaatc actgcaccaa ttagaatacc aaacatcttt aatttaggac ttaactcata
    16561 caaatcatct aaaaaaccag taagggtaat tactgtagca ccaatcacga caggtaaaat
    16621 ataactgaag taggtctgac caaaaaacat ggcgccttta gaagcaatcg gaataagtcc
    16681 caaacttgct actaaaaaag acataaaaat agctaatcca ccactggttg gcatcggaac
    16741 cttattgacc cgtctggcat tgggattgtc tactgcaccc acacgaaagg ctaaaaagcg
    16801 aacaagaggg gttaagaata aggacattaa caaagcccct atcagaacaa ggacataatc
    16861 tattgtgaat gaaaacatac tattttatcc caactcaatc ttttgtaatt gctcaacagc
    16921 accatcaagc atcaattgta agccatgctc ttgtaaataa gctcttgttt ttgatccagg
    16981 gtttgcatgc tcaatcaatc tcgcatacat gacattcgca aaataagatg gttgttgctg
    17041 aacatctaat aaaatagtca tatggtagca gttgctcccc ttatataact ctgaagcttc
    17101 tatcgaaaaa tcaatcgttt tagcaaaagc cactgcctct gtaatcgttg aaaaatccaa
    17161 tacataatgg acatagtcta aaggttctga ctcatgatta gttgatggat cagccgcctc
    17221 agactgatta gctagagtgg cttcaacaac atcttccatt atctcttcaa ttttttccaa
    17281 tttttcatga gctttgacat ctcccttttc acgcatggat tgctccaagc tcttgaaaaa
    17341 atcttccgga gtcatttggg acatatcacc aaactctgct aaatcttcaa gattaatatc
    17401 tttatttatc tcagatttgg taacaaaaac atctagacgg tccttgcgtg gagtcactcg
    17461 aaaactaagc ataccactat ctttaaaatt atctggaaga tcgagttcgt ccatcacaga
    17521 atagaaaaac tcttctgttt tttcttgagg aatcaagaaa tctttcaact ccattcctct
    17581 ttcttctaaa tcgtccatac taattgttat ttttagcgtt gtctcgctaa tttgtttcat
    17641 ttccattctc aatacctcgc acgcgtagtc attttattat actagaaatg ctgtaatttc
    17701 gcaatttatg actaactcct cttctacaaa aggaaattta aaaagcgaca gtccccaaga
    17761 tctgtcgcaa catcctaaac gatatgtaag tttaaaaaac aaatttaaag aagctataaa
    17821 taagtaaaag gcttcctaac acaatacggt attttccaaa aatggtgaaa tcgtgtttct
    17881 taacataatg tgtcaagaac ctaatcgcca ataaactaac cacaaaagcg gtcaagctag
    17941 ccactaaaag aataaggact tgtgcaaaat caaggtgatg gccatcaaga aaaaatttca
    18001 cagcctttaa tccactatat ccaaacatgg taggaatggc caaaaagaag gtgaaatcag
    18061 cggccactgt tcgactagtt cctaaaataa tagctcctaa gatggtagcc cctgatcgac
    18121 tagtaccagg cacaatactt aagacttgaa aacaaccaat gaagaaagct gttttgtaag
    18181 acattctagc taagtcagtg acggcaggtt cttgttgagc attttgtttt tcaatccaga
    18241 taaaggcaat cccgtagacg attaaagcga tggcaatcgg aaccataaaa taaaaatgtg
    18301 cttcaaacca attatcaaga ggaaccgcta ttaaaataga aggaatacac gcaatcacca
    18361 cttttagcca taattgccag gtaagctgta cttctcttgc tgttttccca ggttgaaagg
    18421 gattgagacg ttcaaaataa atcaacataa cagcgataat cgcccctagt tgaataacga
    18481 tattaaacat ttctataaaa gccttatcct gatttagtcg gataaattcc tgcactaaaa
    18541 tgaggtgacc agtgcttgaa actggtaacc attctgtgat tccctcaatg atgccaaaga
    18601 aaatggcttt tagcaattcg attattaaca tgttttctcc ttattaacca ctcttattgt
    18661 gattttctaa actgtgatta atgccaagca gcttttttaa aaggcaccgc cgccgcctcc
    18721 tccgccaccg ccagagaagc caccaccacc actaatcccg cctccggaag agactgagaa
    18781 atttgaggaa gttgtagctg aagacaagct agacacaaag gttggtgtcg tagcatacat
    18841 taccattgat aattcacctg gtcgaacagc ttggtaaacc tcaggtaagg caatacgatg
    18901 gactttcaaa tagcgttcca ctttcttagc gtaaccaaat aaggtggcat aaaccaaaac
    18961 acggttccaa acaaccaaac cttcaatagc aacatcctca aatttgtcaa tgtctcgtat
    19021 catgttttta aagctctgcc attgatgtaa ccgaacaccc ccctcaggtg tttcaatacc
    19081 caatttcttg tattgatcta acttcaggct aatgtaaaag ccaaggcaca tcattaacac
    19141 cattaaagca aggtaaatga gggctaaccc acttcctttc atagtcagat agatcaataa
    19201 actactattc agaacaacta acaagcagcc taacccctgg acactatgac tttttcgttt
    19261 ctctgccggg gtcatttgac gataagtatc tggcaagttt aactttttga tagtttctag
    19321 gacattgttt gtaatagttg ccgacgcttt tttcatcgcc ttaatcacct gctcagacga
    19381 ttgacggact tcttgctcga gctttttacc tttgtacgtt ttctttaatt gtttaatggt
    19441 atcagcatca taatggtatt gactaaacaa ttgatccacc ggcaatgtca ccttgtttcc
    19501 aaaagccata tctaagaagg cagcttcctc atctgtcaca cgatctaact gactgatttc
    19561 aagataagct ttcccttctt ctttcgttaa taaaagcacc ttttggtcga tcagatccaa
    19621 caaggtagct tgaacaaggg cctcaaaggt cactccttta gggatcaata atttctggct
    19681 ttctgatgct gtaggggata aataggcaaa actctgtcca taaatcgctt gtgtcaacac
    19741 aagaggcgaa agatcttcag ggacttcgta actgtgatct gtatggttag ccaaatgata
    19801 acgattgaat tgtttcttta atcttgtaaa ctgtattaac tgcgaaagaa gaaaacctac
    19861 ctctaccaga ggaatgactt tgccaaatag cagctgtaac aaggtccttc gtctggaaat
    19921 cttagtttct aacgctacaa ttttattctt tttactcgtt gaaacatcaa ctggctcttt
    19981 tcccaataat gcttttttat cccaataagc atgcaattcc aattgccctg agacattctt
    20041 ggcattaatc tgatagcgac tgttaccttc ttttaacact tgaggtttct tttggtaata
    20101 tcctctgtga gcccatagat tagaatcctg aatatcagtt ggggtagtga cggtcaaact
    20161 caccttttct aatgttttgt cccaatcact aatcggtgtc caattcagct ctgccacgtc
    20221 ttgataagct gttaaaaggt ggtgcagttg ccagataacc ttgacgtcaa ccttatcacc
    20281 agcttggcca gcattataaa gttttaaacg atagccatct ccaaggtcac taaactcttg
    20341 actaacaggt acctgttgac cattctgata tacctcaact ttaggctttt ggtcaatagc
    20401 aaagcccgct ggcaaatgac ctgtccggcc cagcgaaata tactggccgt tataagaagt
    20461 gtcaaattga taggttactt tttgctcaaa ccgagcagta ttttcttttg ataacagtaa
    20521 ctgaccttca tagttggtga tgctatagtc tacatcagct gcttttattc gtatccctaa
    20581 aagactgaag cagagcacaa gcgtcatcaa aatttttttc atggcaacat cctctttctg
    20641 ccctcatttt accatatttt aaggagatga caaaacctaa accaagtcct tcatcttatt
    20701 tttactcatt attttgaata tttttccata tttcaaccta ttcactgata atatattgaa
    20761 ataggatgca aattgctgta aaataggtct gaatactatt tttgaaacag gagacctata
    20821 tgaaaaagct aatccttagc tgtttggtcg ccttggccct tctgtttgga ggaatgagcc
    20881 gggctcaagc aaaccaatat ttaagagtcg gaatggaagc agcctatgct ccttttaact
    20941 ggactcaaga tgacgcttca aacggggctg tccctattga gggaactagc caatacgcca
    21001 acggttacga cgtccaagtc gctaaaaaag tcgctaaagc tatgaacaaa gaactgttag
    21061 tcgttaagac ctcttggacc ggtttaattc cagcattaac ttctggaaaa atcgatatga
    21121 tcgctgctgg tatgagtcct accaaagagc gtagaaacga aattagcttc tcaaacagct
    21181 actacactag ccaacctgtt ctagtcgtaa ctgctaatgg caaatatgct gaggcaaaaa
    21241 gcctcaagga tttttctgga gctaaagtaa ctgcccagca aggcgtttgg catgtcaatc
    21301 tcttaactca actaaaaggt gctaagttac aaacaccaat gggggatttc tctcaaatgc
    21361 gacaagccct tacttcgggt gttatcgatg cctatatttc tgaacggcct gaagccatga
    21421 ctgctgaagc tgctgatagc cgtttgaaaa tgatcactct taaaaaaggg tttgctgttg
    21481 ctgaatcaga tgctgctatc gctgtcggaa tgaaaaaaaa tgacgatcgt atggcaactg
    21541 tcaaccaagt gcttgaagga ttttctcaaa cggatcgtat ggccctgatg gatgatatgg
    21601 ttaccaaaca acccgtggaa aagaaagccg aagatgctaa agcatcattt ctaggccaaa
    21661 tgtgggctat ttttaaaggt aactggaagc aattcttacg tggaactgga atgacccttc
    21721 tgatttccat ggtcggaacc attacaggtc tctttattgg attattaatc ggtattttcc
    21781 gtacagctcc taaagctaag cataaagtag ccgccttggg acaaaaactc tttggttggt
    21841 tactcactat ttatatcgaa atcttccgtg ggacacctat gattgttcaa tctatggtta
    21901 tctactacgg aacagcgcaa gcctttggta tttcgatcga ccgtacccta gcggctattt
    21961 ttatcgtatc tatcaatacg ggtgcctata tgagtgaaat tgttcgcggt ggtattttcg
    22021 ctgtcgacaa aggtcaattc gaagcagcaa ctgctcttgg ctttactcac ggacaaacca
    22081 tgcgtaaaat cgtgctacca caagttgttc gcaacatttt accagcaaca ggtaatgagt
    22141 ttgtcatcaa tatcaaagat acttctgttt tgaatgttat ctctgttgtg gaactttact
    22201 tctcaggtaa taccgtagcc acacaaacct accaatattt ccaaaccttt acgattatcg
    22261 ccattatcta ctttgttctt accttcacgg tgacacgtat tcttcgtcat attgaacgcc
    22321 gtttcgatgc cgatacttac accactggag caaaccaaat gcagattgcg gaggtctcaa
    22381 atgtctaact caattattga aatcaaaaac ctcaaaaaat cttacggcag caatgaagta
    22441 ctcaaagata tctccctttc tgttaacaag ggagaagtga tttccattat cggttcttct
    22501 ggttctggaa aatcaaccct cctgcgctcg atcaatcttt tggaagagcc aagtgccggt
    22561 caaatcctct ttcatggtga agatgtcctt gctgaacact acaacttgac tcactaccgt
    22621 gaaaaactag gcatggtttt ccaatccttt aacttgtttg aaaacctaaa tgtattggaa
    22681 aatgctatcg tcgctcaaac caccgtcctc aaaagagacc gtgcacaagc cgaacaaatc
    22741 gctaaagaaa acctcaatgc ggttggtatg acagaacaat actggcaagc caaaccaaaa
    22801 caattatcag gggggcaaaa acaacgggtt gccattgcca gagccttatc tgtcaatcca
    22861 gaagccatgc tctttgatga accaacatca gctcttgatc ctgaaatggt tggtgaggtc
    22921 cttaaaacca tgcaagactt ggcaaaatca gggttgacca tgattattgt tacccatgaa
    22981 atggaatttg ctcgtgatgt ttctgaccgt atcatcttca tggataaggg attgattgct
    23041 gaagaaggaa gtcctcaaca aattttcgag aatcctacac aagatcgtac caaagaattt
    23101 ttacaacgct tcttaaaata agataaagga tccaactatt tctataccag ttgggtcttt
    23161 tttcaattca aaaagaattt gtcataaaat actttacca
//
LOCUS       DAUGHY010000028        22394 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.028, whole genome shotgun sequence.
ACCESSION   DAUGHY010000028 DAUGHY010000000
VERSION     DAUGHY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22394
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.028"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(28..2043)
                     /gene="recG"
                     /locus_tag="VH752_001084"
     CDS             complement(28..2043)
                     /gene="recG"
                     /locus_tag="VH752_001084"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEQ8289446.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSVIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDARMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAQNIRKKEVYD"
     gene            complement(2418..3872)
                     /locus_tag="VH752_001085"
     CDS             complement(2418..3872)
                     /locus_tag="VH752_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289447.1"
                     /translation="MERLGGADLDYCYHNHHDTSMISAEQLVFTYHDQKNPACQVPTC
                     QIASGQFIVLCGPSGSGKSTFLKLLNGIIPDYYAGKYEGRLDVADCQAGRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKTIIVAE
                     HRLHYLMDLADNFFYFKNGRLTDKLTTQDLLALTDEQRQDMGLRRLDLSDLKPVLAGK
                     IESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAAGSISGITGSNGLGKSQLVYYIA
                     GILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERHPRTTE
                     VIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQMKALAN
                     LLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            complement(3809..4489)
                     /locus_tag="VH752_001086"
     CDS             complement(3809..4489)
                     /locus_tag="VH752_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ8289448.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWHFPEVWLLTLAVMCRFIPMIRQECCVIHRSLTIRGIILTKWSILIRP
                     KQYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            complement(4486..5079)
                     /locus_tag="VH752_001087"
     CDS             complement(4486..5079)
                     /locus_tag="VH752_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEQ8289449.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPILLMWLLKDAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSAFAHYLLKKHFIKSGLLS"
     gene            complement(5076..6746)
                     /locus_tag="VH752_001088"
     CDS             complement(5076..6746)
                     /locus_tag="VH752_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289450.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGICHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLSVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     KTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLTFGRQDISDESIWNLA
                     EACGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LNEAILLDLIKTHCQGLVFLISHRPSTLACADHLFCVKNGFLKEVNKK"
     gene            complement(6739..8502)
                     /locus_tag="VH752_001089"
     CDS             complement(6739..8502)
                     /locus_tag="VH752_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEQ8289451.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHAIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLPLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQATERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQA
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLSQEIINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGKLLSPGQKQQVICVRALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLSTGQPACLGKPCDLYR
                     DQPTYRHLVDTQARLEASIYG"
     gene            complement(8499..9335)
                     /locus_tag="VH752_001090"
     CDS             complement(8499..9335)
                     /locus_tag="VH752_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289452.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQVWLEQEELATISLKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDIQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGRLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNDIRTQRRDRNK
                     NR"
     gene            complement(9332..10354)
                     /locus_tag="VH752_001091"
     CDS             complement(9332..10354)
                     /locus_tag="VH752_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ8289453.1"
                     /translation="MTASDLQRDQSLLAKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYGAIVKGLFVTYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AITGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYGLAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            complement(10356..11240)
                     /gene="isdE"
                     /locus_tag="VH752_001092"
     CDS             complement(10356..11240)
                     /gene="isdE"
                     /locus_tag="VH752_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEQ8289454.1"
                     /translation="MIKRCKGIGLALMAFFLVACVNQHPKTAKETEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSVNPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDHVGDHP"
     gene            complement(11237..12100)
                     /locus_tag="VH752_001093"
     CDS             complement(11237..12100)
                     /locus_tag="VH752_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEQ8289455.1"
                     /translation="MTKVVIKQLLQVIVVFMISLSTMTGVVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDSQKPVDTKLGESQDESHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKREKK"
     gene            complement(12297..16124)
                     /gene="shr"
                     /locus_tag="VH752_001094"
     CDS             complement(12297..16124)
                     /gene="shr"
                     /locus_tag="VH752_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEQ8289456.1"
                     /translation="MKKISKCAFVAISALVLIQATQTVKSQEALVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKVIVDNDVYEKSLEGERTFDINYQGIKINANLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQGSLKKDVLSDKTVPQN
                     QGTQKVVKSGKNTTNLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDVIITVTKKDGQI
                     HFVSAKQKQHVTAEDRQSTKLDVTTLEKAIKEADAIIAKESNKDAVKDLAEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEGLSRLTSLGFAENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRNHITRIDNISFKNLPKLSVL
                     DLSTNEISNLPNGIFKQNNQLTKLDFFNNLLTQVEESVFPDVETLNLDVKFNQIKSVS
                     PKVRALIGQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTNSAIDKELVS
                     IEEYQQLLQEKGSDTVSLLNDMQVDWSIVIQLQKKASNGQYVTVDEKLLSNDPKDDLT
                     GEFSLKDPGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDLVENGLRALN
                     QKQLRDGIYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVSYLEVEFRGIKVGKML
                     GYLGELSYFVDGYQRDLAGKPVGRTKKAEVVSYFTDVTGLPLADRYGKNYPKVLRMKL
                     IEQAKKDGLVPLQVFVPIMDAISKGSGLQTVFMRLDWASLTTEKAKVVKETNNPQENS
                     HLTSTDQLKGPQNRQQEKTPTSPPSAATGIANLTDLLAKKATGQSTQETSKTDDTDKA
                     EKLKQLVRDHQTSIEGKTAKDTKTKKSDKKHRSNQQSNGEESSSRYHLIAGLSSFMIV
                     ALGFIIGRKTLFK"
     gene            complement(16614..18143)
                     /locus_tag="VH752_001095"
     CDS             complement(16614..18143)
                     /locus_tag="VH752_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ8289457.1"
                     /translation="MNKNKLLRVAMLLSLLAPTAESMTVLAQDVMLETHKATTNETSD
                     SSSKEENNKNAAPTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAVGSSDNKA
                     EAENQVDDKSTDHSKPTDHSKPTDQPKPTDQPKPSPSKVDTAPASSLSRQLPEVRTPI
                     QSLSPYVSDLDLNEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKS
                     TGIAYDSTRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAF
                     DLDPTQASKFNDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFT
                     DTTGSGKRRAQIMEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLD
                     YQASSYAWGQCTWYVYNRAKELGYRFDPFMGNGGDWKYKVGYALSKTPKVGYAISFAP
                     GQAGADGTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSK
                     N"
     gene            complement(18230..19330)
                     /locus_tag="VH752_001096"
     CDS             complement(18230..19330)
                     /locus_tag="VH752_001096"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEQ8289458.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELELAVANAIT
                     VTIASLDWIALARLEKKECQGLKVHVKVDSGMGRIGLRSSKEVNLLIDSLKELGADVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSGSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGVTYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLSKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
     gene            complement(19327..19683)
                     /locus_tag="VH752_001097"
     CDS             complement(19327..19683)
                     /locus_tag="VH752_001097"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEQ8289459.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLSYLAGRWSGKEAFVKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            complement(19799..22318)
                     /gene="secA"
                     /locus_tag="VH752_001098"
     CDS             complement(19799..22318)
                     /gene="secA"
                     /locus_tag="VH752_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEQ8289460.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMASLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLIDEAR
                     TPLIVSGAVSSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAESYFNLS
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEDGEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYNMRIIP
                     IPTNRPIARIDHTDLLYPTLESKFRAVVEDVKTRHAKGQPILVGTVAVETSDLISRKL
                     VEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDRMKLDE
                     EDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDVITANR
                     DLGPEIKAMIKRTIDRAVDAHARSNRKDAIDAIVTFARTSLVPEESISAKELRGLKDD
                     QIKEKLYQRALAIYDQQLSKLRDQEAIIEFQKVLILMIVDNKWTEHIDALDQLRNAVG
                     LRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDVTRTMMKAQIHEQERERASQRATTAAP
                     QNIQSQQSANTDDLPKVERNEACPCGSGKKFKNCHGRKSFS"
BASE COUNT         6903 a         4661 c         3819 g         7011 t
ORIGIN      
        1 atttgtaaaa caacgacttg ttttaagtta atcataaact tctttttttc taatattttg
       61 tgccactaat tgccactgtt tttcatatat ccagttagga tcagaaacaa tcgctgcaga
      121 aactttgcgt gcttcttcta aaataggata atcctcaacg atatcagcta cttgaaattc
      181 tggaattcca gactgacgag taccaaagat ttcaccagaa ccacgcattt ttaaatccga
      241 ctcagctaaa acgaaaccat ctgtcgtttc tgtcatgatt gtcattcgtt ttttccccga
      301 atcagttttg ggattagcca ctaaaacagc gtatgattgt ttatatccac gaccaacacg
      361 cccacgaagt tgatgtaact gacttaatcc aaaacgatcg gcatccataa taatcatgat
      421 cgttgcattt gggacattta cccctacttc aataactgtt gtggatacta aaatatgact
      481 ttttttatcc ttgaaatctt gcattctggc atctttttca tcatttttca tacgtccatg
      541 tacaagagca accttagcaa ttccttcaaa ataagtagat aattcggcat gcaatgctac
      601 tgcattcttt aaatctaaag cttctgattc ttcaatcaac ggtgaaatga catacacttg
      661 agcatctttt tgcaattcac cttttaccca ttccaacaca gtccctagct gctcgtgttt
      721 cacccagcgt gtcataatag gtttacgacc ggcaggtaat tcatcaataa cagaaacatc
      781 catttctcca aaagctgtga ttgctagagt tcggggaatt ggggtggctg tcatcattaa
      841 aacatcagga ttttctccct tttcacggaa aattctacgc tgtttaacac caaaacgatg
      901 ttgctcgtct gtaatgacaa gccccagttt atggtactgt accgagtctt ggataagagc
      961 atgagttcct acaatcatat ccactgagcc atttgcaata gctgctaaaa ccgtacgctt
     1021 gacagcggct ttcatacccg aagttaatat agcaattgat agatctggaa ataactcttg
     1081 cagactaatg tagtgttgtt cagctaggat ttccgttgga accatcaaag ccgattgaaa
     1141 acctgctgta taagctgcat acattgatag accagcaatg actgtctttc ctgatcctac
     1201 atctccttgc aataaacgat tcatatgagc tcccgatgac atatcagata atatgtcatc
     1261 taaagagcgc ttttgagcat ttgttagaat aaagggtaaa gaggaaatct ttgtctccat
     1321 agcatgttta ctataaagaa taggcaaacc atttgtttca gatttatttt cggctttcaa
     1381 aacttgaagg ttcatttgaa agtaaaataa ttcttcaaat ttaatccgac ggagcgcttg
     1441 cttatactct gtgatatctt ttgggaaatg catagctaaa caagcctgac tacgacccat
     1501 caatcggtat ttttccaata aagtagctgg taaattttcc ttcaattcca aatgcgcatc
     1561 gatttcaaaa gctgacttaa tagctttaat caaagtagac tgtgaaattc cctgagctac
     1621 gcgataaaca ggttgcatgt catcttcaac ttgagctaaa accttcatcc cagtaatagc
     1681 cgatttagtg gcatcccatt taccaaaaac agctacctct tgaccaagtt ctattttatc
     1741 agctaaatag ggttgattaa aaaaactaac atttaagaca gcttcccctt gacgcaattt
     1801 gaaacttaaa cggttacgtt taaaaccata atattgtaca ttagctggag taacgactaa
     1861 gcctgtaatg actgcttttt caccatccac taaatcaaaa acagattttg atttaaaatc
     1921 ttcatagcga aacggataat aaagcagtaa atcttctact gtataaatat ctaatttctg
     1981 aaatttttct gctgattttg gtccaaatcc ctttaagttg gacataggag ctgttaatat
     2041 cattatcttt ttcccttcac aaaaactctt gtcagattga agacaaagtt tgtcgtgata
     2101 ctttccttag gtggtgctta cggcagcgac gttctttgga attccctgcc tttatttttg
     2161 agcctgaggt ctcaaaaatc ccgagtggcg tcaatctcgt attgacgcca ctcttttacc
     2221 acagcaaaaa gcatctgttt tggctcactt tatatgtaaa aatcggaaac cgaatgtcaa
     2281 atcagcagag ctatgcatgc tttttgaaca taaaactggg gttgtctacg ttctgactct
     2341 tgtatctatt atacaagagt tcctatttat tttcagataa aagcctttta gtacttttaa
     2401 acagttctag gggctcgtta aggtagcatt tgataaatgg tatcgcagac actacttaga
     2461 agttcttcat catgtgaaat gaggatgaca accttatgtt gggtcttcag ctgcatgaga
     2521 agattagcta aagccttcat ttgtaacaaa tcaagtccgc tacttggctc atcaaaaatt
     2581 aagatatctt tatcagccaa aagactagcg gcaatcatca ctctttgttg ctcacctcct
     2641 gacaaactag ctggatgacg ttctaaaaga gtcgttaaag ataaccgctc tattacttct
     2701 gtcgtccgtg gatgtcgttc atggcccaag ttgacttcct ttgaaaccga ttctgcaaat
     2761 agttgcaaag aaacttcttg caagacaata ctagtctttg ataaacgttg cttggcagat
     2821 aaaggtatgc cttgaaactt aatagttgct tttttatcgt ctaagatacc agctatataa
     2881 taaactaatt gcgattttcc aagcccgttt gatcctgtaa tgccagaaat acttcctgca
     2941 gcaaaactaa gctgttcaat acatctaagt attttgcttc ccgctctaac tgttagatgc
     3001 tcaatacaca ggctatcatc tgggcgataa tgttgactct caatcttacc agctaacaca
     3061 ggttttaggt cagacaagtc taagcgacgt agtcccatat cttgccgttg ttcatctgtc
     3121 aaggctagca aatcttgtgt tgttaactta tctgttaatc taccattttt aaagtagaaa
     3181 aagttatctg ccaaatccat caagtaatgt aaacgatgct cggctacaat aatggttttt
     3241 cctgctgctt ttaattgggt aagataggct ttaacagcag ctatgcctgc actatctaag
     3301 ttagcagttg gctcatcaaa aagcataata ttagtacctt gcataatggc cgttgcaata
     3361 gcaactcttt gtttttgccc tcctgacaat ccaaacatat ccttgttaag gagctctgca
     3421 aaagcaaaat cttctgctaa agtccaaaga cgcttcatga taacctttgc atctagacct
     3481 tgattttcac agggaaagac cagttcgtgt tggacttcgc gatagaaaaa ctgactggct
     3541 ggattttgaa aaacagaggc cacagatcta gaaaatgttt caacactgtc tctgcctgct
     3601 tggcaatcag ccacatctag tctaccttca tattttccag cataataatc aggaattatg
     3661 ccattaagaa gttttaaaaa ggtagattta cctgaaccag aaggtccaca caaaacaatg
     3721 aattgtccag atgcaatttg acaagtcggt acctgacaag ctggattttt ttgatcatga
     3781 taggtaaaaa caagttgttc tgctgaaatc attgaagtat cgtgatgatt atgataacag
     3841 taatccaaat ctgcaccccc caatctttcc atgttaagtg tgagctaaag cattcactgg
     3901 tgcctttgtt gactgctagc cctttagtta gactagcgat agttagttct tggctacttc
     3961 ttatcaaaga taaaagcaat ggaaccatta aatactccaa gtattgttta ggtctgatca
     4021 aaatagacca ttttgttaaa ataatccccc tgattgttaa agagcgatga ataacacaac
     4081 attcttgtct aatcattggt atgaaacggc acataacggc taaggtcaat agccagactt
     4141 ctgggaaatg ccatttgcgt aatccatgaa ccaattcgta gatggtagtt gtcttaatag
     4201 taattaaacc tgctagtaaa gaagggaaaa ctaaccttcc tgctattgag acaaaactca
     4261 ataagtgatc aagataagaa gggaaaatag atagcgggat tacagataga ccgatcatta
     4321 acagataaac aaccgccaac ttgaacgcta gctttttctt atttaaagca gacaaaagta
     4381 ataagagaaa aataacaatt ataaattcta aaaagccatc aacacgaaaa aagaagcagg
     4441 cattggcaag taccaacaaa agcagtttgg tgcgaacatc aagcctcatg ataacaatcc
     4501 tgatttgata aaatgtttct ttagtaaata atgtgcaaaa gctgacccta ataaggctcc
     4561 tgcaagaaca attcctacaa accaaataac atgccctaaa ctaaaatcat acataatgcg
     4621 attgacatag gtcatacttt taccgcgggc taacaaactt tcaacatagg catctttcaa
     4681 caaccacatc agcaaaatag ggccactatt gacaaaagca aaaaccatat aagataggtc
     4741 attaaaaaac cttgacttgt attttcctaa agaagcaata atatccgcta ttaaaccgaa
     4801 aataatgctg ggaaacatcg cggcaaaaaa gtgaccacta aggaagaaaa aacctcccat
     4861 catcagtcct aataatgtta ttggcccaaa tgtttgcaat ttagcaatca aaagaaaata
     4921 aattgttccc cccagtaaag ctgcaaaggc aggggcatat aacatatttc ctgagcgatc
     4981 aaaaaagatg ttaataaacg tgcctagacc aacacaaata aagtagagcg cggcaaatgc
     5041 gcctgttatc ataatatctt ttattgttaa ctgtttcatt ttttattcac ctcttttagg
     5101 aaaccatttt ttacgcaaaa tagatgatca gcacaagcta gggttgatgg cctgtgagaa
     5161 attaagaaga ccaatccttg acaatgtgtt ttaattaaat caagcaagat agcttcattt
     5221 agagaatcta aatttgacgt gggttcatca aaaatatagc aactcgcatc tttcaataaa
     5281 gctcgcatca actctaaacg ttgaccttct ccagctgaaa agtcagaagg actcttgata
     5341 atcgtatcca aaccttcctt acaagctaac agtctgtctt tcataccaca agcctcagct
     5401 aaattccaaa tggactcatc tgaaatatct tgacggccaa aggtaagatt ttcacggata
     5461 gactgttgga aaagctgcgc tgtttgtggc acataagcaa tagtatcttg taatttagca
     5521 gcatttagta agcaactatt cctatcatta agaaaaatat ctcctgtctt ccaattatac
     5581 catttcataa ttaatttcac caaagttgat ttaccagatc ctgattcgcc tttaatgcca
     5641 ataatcccct tttcttgaaa ggtaactgat aaatccttaa aaaccagctc ttggcgcttt
     5701 ggataagcaa aactgaggtc ctcaaaagaa acactcctta aacgatctat ttgttttgtg
     5761 ccttcatcta ctatgacatt ttcgtctaga aggtcaaaaa tatttcgagc cgcattcata
     5821 gcacgcttaa aacccaatgg caaacgtcct agttctaaaa aaggagaaaa cgaagcagtg
     5881 aaagcaataa aagttaataa gcctttatca aatgataagc tgtcatggca tatcccatca
     5941 aaaaccatat aagaaaatgc taagatcatt aagccaagcc agaaaaaagt caatgccatt
     6001 tgcaaaaatt gggcttgcgc tacgttacga tcaagagcat ttacatgatt actttttttt
     6061 attaatcgtt caaattgctc gtctaatacc tgaaattgca aaagatcttt gactgatcgt
     6121 aaactctcta aaaaataaga caggtaatct tttcgacctt cattttggct ttttagtaaa
     6181 acctgtaaaa tattagcaaa ataaatgggt ataacaatgg ctaaacaagc ataggttgcc
     6241 aaagctaata aggctaactg ccaactactt tgacaaaagt accagaacat taaacctgca
     6301 gacaatattg ctgtacaaat aggcgcgatt gtgtgggcaa aaaaaacttc taatgcctca
     6361 atatcttctc caatcatttt aagcaaataa ccactgtctt gactatccaa gcaagctgga
     6421 gaaagagctc ttagtttctt aaagatcaag ttcctaaaag ctgctaaact atgaaaagca
     6481 acaaaatgac caaaataatg ctctccataa cgaacaaaac ctcttaaaaa ggctagagca
     6541 atcaaaagat ataaagctaa tattggaatc acttcttgtc taatagctag tagtcctaaa
     6601 taagctattc cagttggaat acttaccgtg atgacaaaac ccataacagc aaagccaaca
     6661 gccaagataa tccaaggtag cagagttttc attagggtta ataaacgcca aaccaggctt
     6721 aaggtagatc tgttaggttt agccataaat acttgcctcc agtcttgctt gagtatcaac
     6781 taggtgacga taagttggtt ggtcccgata aaggtcgcaa ggtttgccta aacaagcagg
     6841 ctgccctgtg cttaaaaaga gtacttggtt agctcctttc agtcgagaca tcttatgagt
     6901 aatcacaata actattgccg tcttagctaa tctcgttatt aaattgtcaa taatacgttc
     6961 attttctgca tctagactag atgttgcctc atcaaagata taaagagacc tcttacttaa
     7021 tagagctctt acgcaaatca cctgttgctt ttggcctggt gataacagct taccattttc
     7081 accaactatt gtgtcaaggc catctggtag ccagttgatg aagcttaaca aaccatgttg
     7141 atctatccaa tctaagattt cttttttaga gagatttgcg gctaacctta aattatcgta
     7201 aatgctacgg ttcaataagg tagattggtc cgatacataa agaacctgct ggttgataat
     7261 ttcttgcgac aaattgtcac tgtctaaacc gtcaaataaa atatgcccag tcgttgcaga
     7321 ctggcgtttc aagaggagtt gagctagact tgtttttcct tgtcctgaca cccccgcgat
     7381 agctgttagt tggcccttcg ttaatgtcat ggtaacccca ctaagcactg tttttttctc
     7441 ataagctaaa gaaatatttt ggatgtcaat ctggttaaag tttattgctg ttttactctt
     7501 gttatctttc cttgctggaa cactatccaa gaaactaaag atacggtcag ccattttggt
     7561 attcatcatg accaagtgca tgccataacc ctgctcacga atcggtgtaa aaaattctgt
     7621 agcaatcaag agaaaaaaga ggaaattaaa aaaagataac tgacctgttg ccaaagcttg
     7681 aacggctaaa aaacctgaca aaccaattcc cagatacata actgcatcca tataaccaac
     7741 tgcttgtaac tgaaagccaa gcaaagacat ggtagccttt cgaaactgtt ctgccttacc
     7801 cgaaaattct tgctcgtaac gttctgttgc ttgataagaa tacagtgtat taagaccttt
     7861 taagtcatcc ataaataaat taccaacatc catataagaa gaccaataat gactcataat
     7921 ttgcttagac cgcttttgca tagcaacgat tgaaagtgga atcagaggca gaactcctaa
     7981 aaagacaagg cctgccaatg gataaattaa aaagactagt atcataatag ttgtgcagtt
     8041 ccacttggtt ctcatagaca aagacaaata gtaaccataa taagtatcca aactatcaat
     8101 cccctgtgaa gccaacgtta aaatatcagc atcactggca tgagcatcaa attgcccgtc
     8161 taaatcaata aaagcctcaa agaaagattg tttgagtgaa tctctagcaa attgtgaagc
     8221 gatcccttgc agttgcttgc ctaatatagc aagaacaaag cctaccacat ttaaaagtag
     8281 gagtaaacct gccaagcgtc ctaaggcaat agcatgaccc aaaataaagg tcgaaaacgt
     8341 tttggcaatg aggtaaaaac tgatcatcct catcacaaat tgtaaccaag ataaaaaggc
     8401 tgacacataa agcaaatacc tttttggtgc aattcgttca cgtaatcgtt ttaacagtct
     8461 tttccttttt ccccttgata ttgtatcttc tgtttccatc atctgttctt gttcctatct
     8521 ctcctttgag ttctaatatc attggtttca ttgaccaatg aagtgaggac aaactgttga
     8581 ccatttctct caacaaaggg caattcaaca ccaaagacat ctctgataaa ctgttgatca
     8641 aggaggtctc ttggttgccc ttgggcataa agtcgcccat ctttcagtcc aataagatgg
     8701 tctgaaagag acaaggcctg attgatgtca tgtaatacta acacaatcgt taagcctagc
     8761 tcttggttga ttttagtaat cagcttgaga atgtcaagct ggtacttaat atccaaataa
     8821 gtcgttggct catctaaaaa aataatatct gtatcctggg caagggccat agcaatccaa
     8881 accctctgct gttcaccacc agaaaggtgt tggatgtcac gataaacata atcactcaaa
     8941 gccgttactt ctaaggccca agcaaggcgt tcctcatctt ctttggtagt agttttaaaa
     9001 aaagaacgac gggcaagcct agccatggct accaattcac ccactaacaa accttcgacc
     9061 acttgatggt attggtgtac cactgccact tttttcgcaa aagccttgag agaaatcgtt
     9121 gctaattctt cctgctcaag ccaaacctgc ccttgtttta acggaaggtt tttggttaat
     9181 aattgtaata aggttgactt gccactgcca tttgccccca taatagtggt aattttccct
     9241 tttggaatgc taaaggtcag gtcatctaaa acaagttttt cctcataagc aaaggaaagg
     9301 ttttgcacct gaatatgact gctgttctta ctcatataat atcagacctc cttagcaggt
     9361 aaataaagta aggcccgcct acaatactca ttataatcgc agggctaatt tccaatgggt
     9421 aagcaagact tcttcctaaa gtatcggcta gcaaaaagac aaaggctcca agcaaagctg
     9481 aaaaaggaat taagatttgg tgcttacttc caaccagcaa gcggctcata tgagggacaa
     9541 tcaagcctag gaaagaaacc actccaacga tcgaagtggc taccgaagcc aataaaacag
     9601 caaccaagga aattcccaac cgtaacgctg tagcatcgat tcctaaatga cgaatcacct
     9661 gatcatctaa aagaagaagg ttacaggttt ttgacaataa caaagctagt aacaagccaa
     9721 tgaatgtata agggaataaa acccctacat cagcccatgt tttttgacta atatgacctg
     9781 ctaaaagagc attgaccatg ggactagctg aggtgaaaaa actggttaaa gcagttgata
     9841 accccataaa taaagcatta atagcaatcc ctgttagaat aagccgaata ggattaagcc
     9901 ctttcttcca tgctaaacca taaatcagca ggaaagacac tccgcctcct aaaaaggata
     9961 cgattggacc atacagcagc aactgcggta gaagtaaact agagactaaa accataaaac
    10021 tagcaccact acaaatccct gtaatagcag ggtctgaaat aggatttttc aacactgctt
    10081 gaaaaagaac tcccgaaaca gctatccctg ctcctgctaa taaagcaatg acaatccttg
    10141 gaaaacgcaa atcataaatc aaagccactt gggggtcata agtcacaaac agtcccttga
    10201 caattgcccc ataagaaacc gccaaaccac caagggataa ggcaataacc gccaaaaata
    10261 ggaggctgag aattaaaaga actagataaa gaccacttct gaaacgtttt ttagccagta
    10321 gggattgatc cctttgtaaa tctgatgctg tcatgttacg gatgatctcc cacgtggtca
    10381 aaaagctgtg ttaaggtgtc caaggcttct gggtagttca atttagcact catgccaaac
    10441 agggtattgt ccaaatcata gactttccct tccttgactg ccgtaaaatg tttccaaata
    10501 tcattttcag caaattcttt gtcaaacatc acttttacct tgtctggaat ggcgtgagct
    10561 gttcgtaaaa tcaagtcagg ctccttagcc agcatgtctt caggattaac tgatagaaat
    10621 tctttctcat ctgactgata aacattctca ccacctgcca aatccaaaag attccctaca
    10681 taagattggt tcgtcgccac caaataacta cctggcaagc ccataagaat aagcacttta
    10741 ggctttttct tccccttacg tttagcttgg aaagcacgat aatagtcctg gtattgctgg
    10801 cgcaattctt ttgcttcttg ttgacgttgg aaaaggttcc ctaaatcatc aatggactgg
    10861 tacatgccct caacacttcg taagttcaaa aaaccatact cagtatccaa tttttgatac
    10921 ttgggttcca aatcttcttg taaagaattg ggactcaaaa tccaagttgg tttcaaagaa
    10981 gcaatcaact ctatatcagg attcatgggt aaacccacac gcttaacagc atcatagcgt
    11041 ttaggaaggg tatataattt actatcacaa accccaacga ggtctaaatt taaacggtca
    11101 cagatatcaa ccacagcaac cgaagtggct acaattctct gctgttcagt ctctttagcc
    11161 gttttagggt gctgattcac acaagctacc aaaaagaagg ccattaaggc tagaccaatt
    11221 cctttacatc gttttatcat tttttttccc tctttttcca aacccataca gcaaaccctc
    11281 ctaacatcag aaaaaaagtt ggaaagtagt aaatcatgat tgataagtct tttttagacc
    11341 gaaacttatc ttcttttttt aactctaaag aagtcgttaa gcccatctta gaaggaagaa
    11401 tttttttatc tgatagactc ttgttatttg atgagttggc tttaggctta ttctgagtta
    11461 tcatagcacc tgtgtgagac tcatcttgac tttctcctag ttttgtatca acaggctttt
    11521 gactgtcttt tacttcttga ttttgagaat tgtctgtttc agtaacaagc tgggccaaca
    11581 tattacctga atacttttgt attaactctg aagctgacag gtaaaagacc acttctctgc
    11641 ccataggttc aacaaacatg cttcctcgaa tgatactatt tacagtaggc aaactaatag
    11701 caatatctag tgttgtccca ttggtatcag tacctttttg ggtgatattg taatcaactg
    11761 cctgaaagct tccagttcct ccaggttgta tccagaactg ataattgccc gaataatcag
    11821 ctaagctcat acgaaaagta agaacgatct ttccagcatc agatacttct agcatggcat
    11881 cactatagac agtcccttca accatacctt gaccaatatc aaaggaatgc tcccctccgc
    11941 tatcttctat ctgcccagat atcggatgac gataatttct ctgaataata catccataaa
    12001 tttgaccttt atctgcatac acaacacctg tcattgtcga taaacttatc atgaacacta
    12061 ctattacctg caacaattgc tttattacta cctttgtcat atgctcctct tgtctatctt
    12121 ttcttaaaca tcttatttca ttttttaaat gaatcccatt ttataacatc tccaaatgtg
    12181 aaaaacacac ctatcttgcg actacttcaa cctgtatcag gagtcctgtc ggaaataggt
    12241 atgtttttag ctcttgctga ctagcttttt tgttatttca cttggtacta agtttcttat
    12301 ttaaataatg tctttcgacc aataatgaat cccagagcta cgatcataaa gctagataga
    12361 cctgcaatta agtgataacg agagctactt tcttcaccat ttgattgttg attggaacga
    12421 tgtttcttat cagatttttt agtcttagta tcttttgccg ttttaccttc aattgatgtt
    12481 tgatgatcac gcactaactg cttcaatttc tctgccttat cagtatcatc tgtcttagaa
    12541 gtttcttgag ttgattggcc ggttgctttt ttagccagga gatcagttaa gttagcaata
    12601 ccagtagctg ctgaaggagg acttgtaggt gttttttctt gttgacgatt ttgaggtcct
    12661 ttcaactgat ctgttgaagt tagatggcta ttttcttgtg gattattagt ttctttgaca
    12721 acctttgcct tctctgttgt aaggcttgcc cagtctaaac gcataaaaac ggtttgaagg
    12781 ccagaccctt ttgaaatggc atccatgata ggcacaaaga cctgtaatgg cacaagtccg
    12841 tctttcttcg cttgttcaat caatttcata cgcagcactt ttggatagtt ttttccataa
    12901 cgatctgcca atggtaggcc agttacatcc gtgaaataag acacaacctc tgcctttttt
    12961 gttcgaccaa ctggtttacc agctaaatct ctttggtaac catctacgaa atagctcaat
    13021 tcaccaaggt agcctaacat tttaccaacc tttatacctc taaactcaac ttctaaatag
    13081 gaaacacctt ttttaactac caaagtcact cgatgattaa tagccttgtt tgacatggac
    13141 tcagatgcta agtcagtttt taacatgctg gcatttaaat agtaaatacc atcacgcaat
    13201 tgtttttgat ttaatgctct aaggccgttc tcaactaaat ctttctgatg tgaatgtggt
    13261 cctttcgcca caaggatatc attagatgtc aaatagatat gttctttttg agtagcaaat
    13321 ttctttgtta ttaaagcttt gcgaatccga tatgtacctg gatcttttaa agaaaactct
    13381 cccgttaagt catctttcgg atcatttgag agaagctttt cgtcaaccgt cacatactgt
    13441 ccattggaag cttttttttg caactgaatc acaatactcc aatcgacttg catatcatta
    13501 agtaaagaaa cagtatctga acctttttct tgtaacaatt gttgatattc ttcaatagac
    13561 actagttctt tatcaatggc agaatttgtt ttttgctccc aataatacaa atctaaaaga
    13621 ctgaaagctt gatgatattt tattttttcg ccatctaaag aagcttcaag ttttgcaata
    13681 tgtttttgtg gagtcagttt gtgttgtccg ataagagctc ttacttttgg actcacactt
    13741 tttatctgat tgaacttcac atctaaatta agcgtttcaa catctggaaa tactgattct
    13801 tcaacctgag taagcaagtt attgaaaaaa tcaagttttg ttaattggtt attctgttta
    13861 aatataccat ttggaagatt tgaaatttca ttagttgata aatcgagtac agataattta
    13921 ggaagatttt taaatgaaat attatcaata cgagtaatat gatttctact tgccacaata
    13981 gtgcttaagg cgcgcaattt ttctattgtt tttggcagta gtgctaaatt attttgagat
    14041 aagtcgataa aattaagtga tgttagaggc tctagcgctt tttcaggtat ctcctcaaga
    14101 ctattttctg cgaaacctaa tgaagtcaga cgagacaatc cttcaaatgg gttatcacaa
    14161 agtcggccaa tacgattctt ggaaagatca agttgctcca ggtgatgaag cgactggaat
    14221 agtcctcctt caagagagtg aataaagtta cttgataagt agagttctct taattgagca
    14281 ttgcttttaa atgtatcttt gtctagataa gttaaatgat tacttcttaa gttaagaaat
    14341 cgaagttgct tcatttgact aaataactct tttggaattt ccgtaatttg gtttccatcc
    14401 agatgtagtt ctgtaatatt tttagcatgt ttcaataaag aaatatctgt taactcgtaa
    14461 tggtctagac gcaactcacc tcgaatctgt tctaattccg tttttgataa tttaccatcg
    14521 ccgtctaaat ctttgccaag tttaactaaa actttttcaa gacgttcagt cccaacccct
    14581 ttttctttat catcagtttc tacctgataa ccactccaac ctttggtaac ggtcttacta
    14641 aaggtcatgt caagtttggt gtatttgtca tagtggttta aatcactttc ttgacctccc
    14701 atggctgata ccaaaacagc acctttgtgt aatttatcca aatcatcaat aggcatggta
    14761 aattcgcttc gaatatagct accattgata tctttttggc caacttgttt acgcactgct
    14821 gctgggtagg tccctttgct ttcaatagaa aagtcaatca aaaattgtcc caaagcagta
    14881 ttaagcatgg aaatttccat tgtaccatct gctttaacca ctaatttggc tcttttatca
    14941 aaagcacctt gaagcatgga ggactcttct gagttttctt tattagcttt aaagtttagc
    15001 gtataggttc cttctgtgag attgttaata gaaacctcac ctgcttgata agactcgatg
    15061 gtatctttta acagtcgatg agtatcggcg agtagcttac tatctttgat ttctttgtaa
    15121 gaatccttga tgacttgaag tttctcagcc agatctttga ccgcgtcttt gttgctttct
    15181 ttagcaataa tcgcatccgc ttctttgata gctttttcca aagtggtgac atccaacttt
    15241 gttgattgtc tgtcttcagc agtcacatgt tgtttttgtt tggcagatac aaaatggatt
    15301 tgaccatctt tcttggtaac ggtaatgata acgtcttcaa atccttttga ggatatcacg
    15361 attgtatttt ctccatcttt gaaagctttt gttccaagat gaagccctgt catattcgag
    15421 acccagccat tagtatcttt tacagaatct gaaataagat ctttataaac tgtaccattg
    15481 actgtaacct ttgtgatttg ctgagtcaat gcttttatct gtttgttatc agaataacga
    15541 tcaatttctg gaaataaaat tgcaccatct tcttgactca attttgttat taatgacaag
    15601 ttagtagtat ttttcccaga tttaacaact ttttgtgtgc cttggttttg tggcactgtt
    15661 ttatcactga gcacatcttt ttttaatgaa ccctgatgat ctgttgttcc taatttttga
    15721 gctgaaataa aggtcacttt atcgcccttt ttaataaagg taattaggat atcaccatct
    15781 tttttattaa caatagtcaa ttcatgctta ccgtctttaa taaggttagc attgatctta
    15841 atcccttggt agttaatatc aaaggttcgc tcgccttcca ggcttttttc atagacatca
    15901 ttatcaacaa taaccttttc aatatgttta taatactctt ttccagctga ttgactagcg
    15961 taagggccta tctcttcaac taaaagtcga ttatcctgag ttaaagctac tgttgtcacg
    16021 agttgtgact gaactaaagc ctcttgtgat tttacagttt gagtagcctg aatgagaaca
    16081 agggcagata ttgccacaaa cgcacatttg ctgatttttt tcattttcaa tcaactttct
    16141 aaaaattttt atgaataagc taactttttt attaactgaa cttaatctat cactttgtga
    16201 ttattttgtc aagattgttt ccaatggtcc aattgtctgt taaatagctc cactatgtga
    16261 gctaagacac tagtaataag cctggattga taatgaggat atctctgata aaagaattcc
    16321 tctacaacaa tattactgag ataaagctag ccttaggata gttattacta gggctgattc
    16381 aatcattact tcattcacaa aacatgtaac acgttataag ctccataatt gtgttaggag
    16441 acaacatggg atgtcactac tgcatattca tcacaataaa aaaagacaat agccctaatc
    16501 gtgctattgt ctcaaaatca tttatttact tgaaacttta tcgtgttaca ccaacagttt
    16561 aaaaggccta actcccgcta agaaaattaa gccgtaagaa aagtcattag ttattagttt
    16621 ttactcttgc caataacata tgttagctgt tcagcctgct gagctgtaaa ggtacgataa
    16681 gaaatcttac ctaagccgat acagttagac tctgaaataa gaatagaccc atcttttcta
    16741 acatcttcta caattgatac gtggccataa gtgccatcag cgcccgcttg ccctggtgca
    16801 aatgaaatag cataacctac ttttggagtc tttgaaaggg cataccctac tttatacttc
    16861 caatctccac catttcccat aaaaggatca aatcggtaac ccaattcttt ggcgcgatta
    16921 tacacatacc aagtgcattg tccccaagca taactcgaag cttgataatc caagacatca
    16981 taactcttag agagcttata acctgctggc acagatgcaa aactagctga tgactgcact
    17041 ttcaattcgg ctggaatggc tggtgtgcca ccatggtcat caatccactt atccaggtct
    17101 tccataattt gtgcgcgacg tttaccacta ccagtagtat cagtaaaata aacacccccg
    17161 tcacttgcaa agtttaattg acctcgtgaa aacttagccg cttttaaatc ttgaagtgca
    17221 aaattgctat ttttgttttt aataatggta tcttgtgtca ttttgacaat agcactatca
    17281 tcattaaact tacttgcttg agtcggatct agatcaaaag ctgcatagcc aaacatatta
    17341 gctccaagca aagtcgcaat cccttgagtt cctaaagaac tctcagacat cgcaatagct
    17401 acgatagctc gaacatccag cccacttttc ttttcccatt gcaatagctt ctcaccatta
    17461 atacgtgtgc tgtcataggc aatgcctgtt gattttaagt aactatcaat ctggtcagct
    17521 ttaataccat aacggcgaga taaaagatgg tgggtatagg catttttacc actccaatgc
    17581 tctacatatg ccgcgtatgt gttgacagaa gggatatcta tctcattcaa atctaaatct
    17641 gatacgtaag gtgacaacga ctgaatagga gttcttacct ctggcaactg tctcgacaat
    17701 gaagaagcag gtgccgtatc aactttagat ggtgatggtt tgggctgatc agtcggtttt
    17761 ggctggtcag ttggtttcga atgatcagtt ggtttcgaat ggtcagttga tttatcatct
    17821 acctggtttt ctgcttctgc cttgttgtcc gaagaaccta cagcagactg actagaatcg
    17881 cttctttttt tatcatccgc gttaacaaga ctattagagt ttgtttctgc agaagcatca
    17941 aggggacctt ggtcagtttt atctgatgtt gtaggtgctg catttttatt attttcctct
    18001 tttgaagaag aatcactggt ttcatttgta gttgctttat gcgtctcaag cattacatct
    18061 tgagccaaca ctgtcatgct ttctgctgtc ggggctaaga gacttagtag catggcaact
    18121 cttaatagtt tgtttttatt catatattaa ttagtcactt ctttctaatg tcctcacttt
    18181 attataacat taaagataag cacaatgcaa ttatttttac gtgattttct taatatatcc
    18241 gaggaatacg gtcacttaaa aggcatagaa cttcataatt gattgtatta cggtaatttg
    18301 cgatatctgt tgtagaaata tttttttgct gattgctgcc aatcaaagtg acttttgttc
    18361 ctaaaggata tgctttggaa agtcgtatgg tcagttgatc catcgataca cgccctataa
    18421 tttcacagaa ttgtccatca actaacaccg aaaagccttg catgttcctg gtccagccat
    18481 ctgcataacc gattgggacg gtccctacat attcagactt tttggcagta taagtaactc
    18541 cataaccgac tgtatcacca gctgaaatca tcttgacatg cactaagcta gattctagag
    18601 ataaagcctc ttgcagtgga aatggtaagc ttagatcaga accacttgga ttcaaaccat
    18661 aactgacaat tcctaaacga acagcattaa aaatggtatc actatgccag atacttgtgg
    18721 ctgaattact agcatgtact aaacgaggct tatcctcaag tccagctatc agctttttaa
    18781 aaaactgtaa ctgctggtta aatttagtat catctgcctc atcagctgta gcaaaatgag
    18841 taaaaatacc ttctacatca gcacccaact cttttagact atcaatcaat aaattgactt
    18901 cttttgaaga acgaagcccg atccgcccca taccagaatc aacttttaca tgaactttta
    18961 agccttgaca ttcttttttc tccagtctag ctaaagctat ccagtctaaa ctagcgattg
    19021 taacagtaat agcattagca actgctaact ctaattcatt tggcagcaaa accccaagaa
    19081 ttaaaatctc tttatcaata cctgcttgac gtaattgcaa agcctcatca agatttgaca
    19141 cacagtaccc atccacttga ggtaggagtg cttttgacac ctggacagca ccatgaccat
    19201 aagcatcagc cttgacaact gcatacgttt ttacccctag tggaatatgc ttttgaacac
    19261 tggcaacatt ctccttaata gcttgtaagt tgacccttgc aactgttggg cgatggaaac
    19321 ttgaaatcat tttttatcct ccaaaataac actagcctgt acataattgc cactatgtga
    19381 gatgctaata aaactatttc ctttaaacgg agattttgtc agaatgggac atcctctaac
    19441 atcatttaaa atctcaatat cttgaaaggt taaccgccca atacctgtcc ctatagcctt
    19501 aacaaaagct tccttacctg accagcgtcc tgccaaatag ctgagtcgac gtttgtaagg
    19561 aaaactctca aaaatagcca attcctgctc agttaaaatt ttttgagcaa agcgaggatt
    19621 tctttgataa actttttcaa tagctgaaat ctcttgtaag tctattccat gtccaacaat
    19681 cattactttc caccaaacct tatttcatct taatgttaga agatttaaac agtgagaagc
    19741 tgagaattat tctcagcttc tttctatata cctaactttg tcctgttgtg tctcgttctt
    19801 atgagaagga tttacgaccg tgacaatttt tgaatttttt gcctgaacca catggacaag
    19861 cttcgttacg ctccactttg ggtaaatcat cggtgttagc tgactgctgc gattggatgt
    19921 tttgaggtgc tgctgttgtg gcacgttgac ttgcacgttc acgttcttgt tcgtgaattt
    19981 gtgctttcat catggtacgt gttacatcaa actcgatagc accaatcata tcttggaaca
    20041 tcttgaaacc ttctgcttgg tattccacca cagggttatt ttgagcatac ccccgaagtc
    20101 caacagcatt acgaagttga tctaaggcat caatgtgttc agtccatttg ttatccacaa
    20161 tcatcaaaat gagtactttt tggaattcaa taatagcttc ttgatcacga agttttgata
    20221 gttgttggtc atagatagca agcgctcttt gataaagctt ctctttgatt tgatcatctt
    20281 ttaaaccacg taactcttta gcactaatag actcttctgg taccagcgag gtcctagcaa
    20341 aagttacaat agcatctata gcatctttac gattgctacg tgcatgagca tctacagcac
    20401 ggtcgatagt acgcttgatc atagctttaa tttctgggcc aagatctcga ttagcagtta
    20461 tgacatcacg acggttagca tagataattt cacgctgttc acgcatcaca tcatcgtatt
    20521 gtaagacctg tttacgggta tcgtagttat ttccttcaac ccgtttttga gcagattcca
    20581 cttgacgccc taacatacct gatttaataa cagtgtcttc ttcatctaat ttcatccgat
    20641 ccaaaaaggc ttttatacga tcagagccaa aacgtctcat caaatcatct tctaatgaaa
    20701 gatagaattg ggattcacct ggatctcctt gacgacctga acgtccacga agctggttat
    20761 cgatacgacg gctttcatga cgctctgtac caatgacaca taacccacca agttcacgaa
    20821 caccttcacc aagcttgata tcagtaccac gaccagccat gttagttgcg attgtaacag
    20881 caccacgttg cccagcattc ataatgattt gagcttcttt aaagtgattt ttagcattca
    20941 aaacctcatg aggaatccct gcttcaacca gcttgcgtga aatcaaatca ctagtttcaa
    21001 cagcaaccgt acctacaagg ataggttgtc cttttgcatg acgcgtttta acgtcttcca
    21061 caactgctct aaactttgat tctaaggtcg ggtaaagcaa atcagtgtga tcaatacggg
    21121 caattggacg attggttgga attggaataa tacgcatgtt ataaacttcg cgaaattctt
    21181 cttcctcggt cttcgcagta ccagtcatac ctgccaattt tttgtacata cgaaacatat
    21241 tttggtaagt gattgaggcg ctggtttttg attcttcctg aatacgaacg ccttcttttg
    21301 cttcaatagc ttggtgcaag ccgtctgaga agcgacgccc ttccatcgta cgacctgtaa
    21361 attggtcaac aataagaatc tcaccatctt cgctgaccac ataatcgata tctaacaaca
    21421 taatatagtt tgcacgaagg gcgttgtcta taaagtgagt caaggctaca ttttcaatat
    21481 cgtaaagatt gctcaagtta aagtaactct cagctttatc aatacctgaa tcgcttaatc
    21541 caatcgtttt agtgggcaca tcaataacat aatccactga cgtcagagtt tttacaaaca
    21601 tgtcagcacg aatatacaat tgattggttt cagagctaac agcaccagaa acaattaaag
    21661 gtgtccttgc ctcgtcaatt aagactgagt ccacttcatc aaccaaggca aaattgagtg
    21721 ggcgttgtac catgtcttcc tggcgaacaa ccatgttgtc acgaaggtaa tcaaaaccga
    21781 cttcagagtt ggttgaataa gtaatatcac aattataagc ttcacgtttt tcagcaggag
    21841 acttagcagc taggttgatc ccaactgaaa gacctaacca gctatacact tcacccattt
    21901 cggtagcgtc acgcgttgaa agatactcat taacagtaat aacgtgaaca ccctcaccag
    21961 caatagcatt gagataaact ggcatggtgg ctgtaagagt ttttccttca ccagtacgca
    22021 tttcagggac atccccgtta tgaagaacaa ttcctcccat aatttgaaca cgataaggga
    22081 acaagcccaa cacacgtttt gctgcttcac gaacaactgc gaaagcttca ggtaatagtt
    22141 gttcgagggt ctcacctttt tgatagcgtt ctttaaattc aagtgttttt ccttgtagat
    22201 ctctatccga caatgacgcc atttggtctg cgtaggattc tacttttttt gcaatttttt
    22261 ctaattttct tagttcgcct ttgtcatttt cgataacttt gcgtagaata ttggccattc
    22321 tctttccttt cgatcatcta atcatttcat tttaacataa atatgcaggc agttcaagaa
    22381 aggttggtaa agtt
//
LOCUS       DAUGHY010000029        22277 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.029, whole genome shotgun sequence.
ACCESSION   DAUGHY010000029 DAUGHY010000000
VERSION     DAUGHY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22277
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.029"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(347..532)
                     /locus_tag="VH752_001099"
     CDS             complement(347..532)
                     /locus_tag="VH752_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289461.1"
                     /translation="MNKLELFLLVTTIILAIITRVQHEVIKKHNSPENKRRIFREVAL
                     ENSKGWSEKRSGGKVVS"
     gene            complement(534..845)
                     /locus_tag="VH752_001100"
     CDS             complement(534..845)
                     /locus_tag="VH752_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEQ8289462.1"
                     /translation="MPKAEITYRAVDVNETASHGDYKHLIQQWEGLTVATAKQWATEM
                     RDHPDFKQFVLNPTHRIVFIDYEGFKLFVQWKSRNRYKTKKETLSEMLENIKFEKRVG
                     A"
     gene            988..1224
                     /locus_tag="VH752_001101"
     CDS             988..1224
                     /locus_tag="VH752_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289463.1"
                     /translation="MIFFFLSVVSKHINQNFNVDNQLVEFVVAELNQMNAPITQKQAQ
                     HIVNILEYVSKSTSKSTIAAMTNALLELGVLKGD"
     gene            1366..2172
                     /locus_tag="VH752_001102"
     CDS             1366..2172
                     /locus_tag="VH752_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEQ8289464.1"
                     /translation="MLNIDTQFVDTIGKILSDYVSHSEITRMGEVLGYPQNDQNSGFN
                     KHKRVHNLVSDILNRTQNTDNIKLVIEYVCNPLRYINEVSIFEQLRTAINIPLSLKGL
                     IVSDSGQIVTTTTSKTLSEAKKRFETLDSRLKELKVHPHVLKFCTQELLQENYFHAVF
                     EASKGVFHRIRLLTGSAMDSASLIDQCFKPGEPIVIINGNKLQTLDEQSEYKGLKNLL
                     LAIAHLYRNSKAHKLKYYNPDNLNDALTALTLMSLAHNLLDSCSNTRRLD"
     gene            complement(2107..2373)
                     /locus_tag="VH752_001103"
     CDS             complement(2107..2373)
                     /locus_tag="VH752_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289465.1"
                     /translation="MEEKKFFTTYEMDLMNSVVTLQKSILTQTEHLSDQLTKKLHHLE
                     DYNSPIDDEAIRLAEVTAEFYKLLIKSPSVGATVKEIVSEGHKR"
     gene            complement(2405..3130)
                     /locus_tag="VH752_001104"
     CDS             complement(2405..3130)
                     /locus_tag="VH752_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEQ8289466.1"
                     /translation="MNHLINVTLNENQEPVVSARDLHKELKVKTRFSEWVKQNFKILE
                     EGYDFTSVVGTTVVNNGAVREIQDYVLSLDAAKNLAMVSKTDKGKEVRKYFIQVEKDF
                     NSPEKIMARALLMADKKVHKLESQIEADRPKVLFADAVSASHTSILVGELAKLLKQNG
                     VNIGATRLFTWLRKHGYLIKRNGRDWNMPTQKSVELGLIRVKETSITHSDGHITVSKT
                     PLVTGKGQQYFINKFLNQEYLPV"
     gene            complement(3181..3408)
                     /locus_tag="VH752_001105"
     CDS             complement(3181..3408)
                     /locus_tag="VH752_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEQ8289467.1"
                     /translation="MTKKQRLKQEHLKPKKRLREERLKREYTEMYMADLIGLKNRKGY
                     SEKEDGLQPFKDYEMAIISQKFNVSESELFF"
     gene            3568..4323
                     /locus_tag="VH752_001106"
     CDS             3568..4323
                     /locus_tag="VH752_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ8289468.1"
                     /translation="MNILGNSIKEIRKSKKMTQKELAKLTGFKQNTISNHENGNRQLD
                     EVDIRKYAKALKIEPQQLFDYSSSPTNPQVELIPSTLQKITSTSSQLEHKRQLNVLDY
                     AETQLEQQNTVESNKDIVEEPQATYYTYNYYDHAASAGTGQYLNDVQVETIELPVDYD
                     ADFVIPVHGDSMEPKYHSGDYVFVKLSVELADGDIGVFEYYGDAYIKQLLINDEGAFL
                     HSLNSKYEDIPVDRDSDFRIIGEVVGSYSGNHS"
     gene            4361..5041
                     /locus_tag="VH752_001107"
     CDS             4361..5041
                     /locus_tag="VH752_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289469.1"
                     /translation="MKFWNLVVKTLKQKNNQAVAEKVVKITESTTNIKSPSYPDLNKY
                     RVNPSGKRYDDTYITGVGYKLREILLLVWWGRTKNPRKPTSKPPRYFFYDYHLNTKKT
                     TDMFIRDGLLKKNKEGCITLTLSGKVLYDEYKILWEIHSYKGYIGELPNMDRVFHGWN
                     YNSYKANNNLLEIRHLEDIVKYNTIMRDQYKKGSNEYNAFQQDIEQDKNQIALLFNEH
                     QLLENIDN"
     gene            5177..6316
                     /locus_tag="VH752_001108"
     CDS             5177..6316
                     /locus_tag="VH752_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ8289470.1"
                     /translation="MWSEKHKSGKVNFVERYKHPYTGKWCRASVLMEKDTPRIRKEAK
                     KYLDEKISKILTSLTTTDAYLLDVMNEWWEHHQKSLKSTSIRSLDFRIRELRNLIDPE
                     VMIAKITTKYLQSIIDKIPGSYDKRKRARQLLKQTFDYAIALEYVSINPVISTQLAKP
                     VKTIKDFEDVAQKFLEKDELKRLLDEMYRRKGSIKMAYLAEFMSLNGCRIGEALAIQP
                     DNIKNDIIEIHGTLDYTSNGYRNAIKTTPKTNSSWRETLITKREKEIIQDILKINALE
                     KNTNPNYKDMGYIFISRNGVPIQDNALNTSIRAANKRLEKPIQKELTSHIFRHTLVSR
                     LAENKVPLKTIMDRVGHADSKTTQQIYTHVTKSMKNEVVDILNRL"
     gene            complement(6410..7450)
                     /gene="rfbB"
                     /locus_tag="VH752_001109"
     CDS             complement(6410..7450)
                     /gene="rfbB"
                     /locus_tag="VH752_001109"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEQ8289471.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKTDAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLYGE
                     GKNVRDWIHTNDHSIGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(7694..8287)
                     /locus_tag="VH752_001110"
     CDS             complement(7694..8287)
                     /locus_tag="VH752_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEQ8289472.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYAFVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            complement(8287..9156)
                     /gene="rfbA"
                     /locus_tag="VH752_001111"
     CDS             complement(8287..9156)
                     /gene="rfbA"
                     /locus_tag="VH752_001111"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEQ8289473.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTSQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLERGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHELAQSLKKN
                     EYGQYLLRLIGEA"
     gene            complement(9214..10320)
                     /locus_tag="VH752_001112"
     CDS             complement(9214..10320)
                     /locus_tag="VH752_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEQ8289474.1"
                     /translation="MKIAIIGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLINDLKEDGFATDFYQQNGIYVLKKQEEKLRD
                     LYELALARKVESPIIGELAIKNRKELGNDFKGLIGFDNCLYASGAARVEGATLCETLL
                     KASGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLRPLGYQVDVRPQKGQ
                     LLDYDVHHIISDNYPVVMPEGEIDLIPFNQGKISVGASHENDKGYDLEPDWQVLEKLE
                     MQALTYLPLLKEATQKTCRVGIRAYTSDYSPFYGQVPGLKNFYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLRSNGKA"
     gene            complement(10355..11143)
                     /locus_tag="VH752_001113"
     CDS             complement(10355..11143)
                     /locus_tag="VH752_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEQ8289475.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMEGDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDLIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISVYVSHTNID
                     IVPGGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            complement(11133..11819)
                     /locus_tag="VH752_001114"
     CDS             complement(11133..11819)
                     /locus_tag="VH752_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEQ8289476.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIA
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            complement(11891..12547)
                     /gene="nth"
                     /locus_tag="VH752_001115"
     CDS             complement(11891..12547)
                     /gene="nth"
                     /locus_tag="VH752_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEQ8289477.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
                     "
     gene            complement(12544..13227)
                     /locus_tag="VH752_001116"
     CDS             complement(12544..13227)
                     /locus_tag="VH752_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEQ8289478.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFYLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTSQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISEDFL
                     SAMNLWSDS"
     gene            complement(13308..13826)
                     /locus_tag="VH752_001117"
     CDS             complement(13308..13826)
                     /locus_tag="VH752_001117"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEQ8289479.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            complement(13977..16187)
                     /gene="recJ"
                     /locus_tag="VH752_001118"
     CDS             complement(13977..16187)
                     /gene="recJ"
                     /locus_tag="VH752_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEQ8289480.1"
                     /translation="MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYDRGIRTE
                     EALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEEGERILIYGDYDADGMTSASIVK
                     ETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
                     EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPT
                     DCLDLVAIGTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDA
                     IGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQA
                     MAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAI
                     NIFEALNGKRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTI
                     DERLSLDDLSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQARTIGQDQSHLKFKVSQ
                     GKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLD
                     LRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKHPY
                     YLTGFGSREQFAKLYKTIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVT
                     IDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            complement(16184..16948)
                     /locus_tag="VH752_001119"
     CDS             complement(16184..16948)
                     /locus_tag="VH752_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEQ8289481.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKMVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAQEIADM
                     FQVNTLASIHFACLIGQKMAEQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFDQADPSGHYLESVGTFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYLARKVFNYK"
     gene            complement(16948..17877)
                     /locus_tag="VH752_001120"
     CDS             complement(16948..17877)
                     /locus_tag="VH752_001120"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEQ8289482.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFEVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            complement(17892..18527)
                     /locus_tag="VH752_001121"
     CDS             complement(17892..18527)
                     /locus_tag="VH752_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEQ8289483.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            complement(18520..19758)
                     /gene="hflX"
                     /locus_tag="VH752_001122"
     CDS             complement(18520..19758)
                     /gene="hflX"
                     /locus_tag="VH752_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEQ8289484.1"
                     /translation="MIETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRERRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDIAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            complement(19831..20730)
                     /gene="miaA"
                     /locus_tag="VH752_001123"
     CDS             complement(19831..20730)
                     /gene="miaA"
                     /locus_tag="VH752_001123"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEQ8289485.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DIGTAKATQEEQEAAVHHLIDIREVTESYSAYDFVQDAQKAISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
                     ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVNRMLENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            20864..21040
                     /locus_tag="VH752_001124"
     CDS             20864..21040
                     /locus_tag="VH752_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEQ8289486.1"
                     /translation="MVKKYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            complement(21158..22066)
                     /locus_tag="VH752_001125"
     CDS             complement(21158..22066)
                     /locus_tag="VH752_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEQ8289487.1"
                     /translation="MKHLKNPPLVMSGLALGTLSFGNLLATYVSIFSYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGVLLSSLFLKVTGGCWLGFLTWWLF
                     FLGNLVLIAYYQYRFVFSFSWDNVFPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LPLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     ISSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQGLGWQVLL
                     LGEVLLATALVTYVYGAYLRFLFQNK"
BASE COUNT         6774 a         4742 c         3923 g         6838 t
ORIGIN      
        1 taaaacgtta agttgtaaaa tcactaatca tttttttaaa tgcttgttcg gatatctttt
       61 tatctgagca aaatctataa tattgtatgc ctttccctcg ccctaaagat tttctgtaag
      121 ttctcacata tccgactctc tctaattctt ctaagcaagt ccttacaact ctcacgctat
      181 ctctggacct tctagccaat tcctctggat atactctcca atcttcttta tttctaagaa
      241 ttaccataag caggcctttt gattttaggg ataattcaca atcatctata aattcgttgc
      301 taacggatgt atagctactt gtcgtatttt tgaaaaatgt actgcatcag ctgaccacct
      361 ttcctccaga acgcttctcg ctccaccctt tgctgttttc taaagccact tctctaaaaa
      421 ttctgcgttt attctctggc gaattatgtt ttttaatgac ttcgtgttgt actctagtga
      481 tgattgctaa gatgatcgtt gttactaata aaaatagttc taatttgttc atgttatgct
      541 cctactcttt tttcgaattt aatattttca agcatttctg acagtgtctc tttttttgtt
      601 ttataacgat tgcgagattt ccactgtaca aacagtttga atccttcgta atcaatgaat
      661 acgattctat gcgttggatt taatacaaac tgtttaaagt ctggatgatc acgcatttct
      721 gtcgcccact gctttgcagt agcaactgtc aacccctccc attgttgaat caagtgttta
      781 taatcaccat gagaagctgt ttcattaaca tcaactgctc tataagtaat ttctgctttt
      841 ggcatattaa tatcctctct cttgtggtat aattaagtaa attaatctag ttttgagtcc
      901 gattgccgtc ggactttttt tgttatctga attcgtccaa gctgacacct aagacatcgg
      961 cgattttaac gacatcatca aatttcaatg atttttttct tcctttctgt tgtctcaaaa
     1021 catatcaacc aaaatttcaa tgttgacaac caattagttg aatttgtcgt tgcggaactt
     1081 aatcaaatga atgcacctat tacacaaaag caagctcagc atatcgttaa tattttggag
     1141 tatgtctcta agtcaacctc taaatctact atcgcagcta tgacaaatgc attgttagag
     1201 cttggcgtac ttaagggaga ttgatgcaat caactttacc ggtttttatc agttcagggt
     1261 ctatcttatg ataggctctc tttttctctc cgctatacgg atatcgtttt ggtctcatgt
     1321 ggttctcctt tctgtggtat aatttaaata aaaacgaggt ttgttatgtt aaatattgac
     1381 acccaattcg ttgatacaat cggtaaaata ctatctgact atgtttcgca ttctgaaata
     1441 acccgaatgg gcgaagtttt aggttatccc caaaacgacc aaaattctgg atttaacaag
     1501 cacaaacggg tgcacaatct cgtgtccgat atattaaata ggacacagaa tacagataat
     1561 ataaaacttg tgattgaata cgtctgtaat cctttaagat acattaatga agtttcaatt
     1621 tttgaacagt taagaaccgc cattaacatt ccgctctcgc taaaaggttt gattgtatca
     1681 gatagcggac aaattgttac tactactact tcaaaaactt tatcagaggc caaaaaacgc
     1741 ttcgaaacac ttgatagtag attaaaagaa ttaaaagtac accctcacgt tttaaaattc
     1801 tgcactcaag agctcttaca agaaaactat tttcatgccg tatttgaagc aagtaaaggt
     1861 gtttttcatc gtattcgtct gctaacgggc tcagcaatgg actcagctag tctgatagat
     1921 caatgtttca aacccggaga acccattgtg attatcaacg gtaataaatt gcagactcta
     1981 gacgaacaaa gtgagtataa gggattaaaa aatttgctac ttgcaatcgc tcacctttat
     2041 cgcaactcta aagctcacaa actcaaatac tacaatccag ataatctcaa tgacgctctt
     2101 acggccttaa cgcttatgtc cctcgctcac aatctccttg acagttgctc caacactagg
     2161 agacttgatt aacaacttgt aaaattctgc tgttacctca gccaatctaa ttgcttcatc
     2221 atcaatcgga ctattgtagt cttctaggtg atgaagcttt tttgttagtt gatctgacaa
     2281 atgttccgtt tgcgttaaga ttgatttttg aagagttaca actgaattca tcagatccat
     2341 ttcatatgta gtaaaaaatt ttttctcttc catattttct cccttcgtta gttttgttac
     2401 gtttctaaac tggtagatat tcctgattaa gaaatttgtt gataaagtat tgctgacctt
     2461 ttccggttac cagaggtgtc ttgcttactg taatatggcc atcagaatgg gtgatactag
     2521 tttccttgac tctgataagt ccaagctcta cgcttttctg agtaggcata ttccaatcgc
     2581 ggccattgcg cttgataaga tagccgtgtt tacgaagcca agtgaagaga cgggttgctc
     2641 caatatttac cccattctgc ttgagtaact ttgcaagttc tccgactaag atagaggtat
     2701 ggctagcact tacggcatca gcaaatagta ctttgggacg gtccgcctca atctgagact
     2761 ctaatttatg cactttcttg tccgccatca gcaacgccct tgccatgatt ttctcaggac
     2821 tgttgaagtc tttttcaacc tggatgaagt atttacggac ttctttgcct ttgtcagtct
     2881 tggataccat tgccaaattt ttggcagcat caagtgagag aacgtagtct tggatttctc
     2941 tgacagcccc attatttaca accgtagttc caactacact tgtaaaatca tatccttctt
     3001 caagaatttt aaagttttgt tttacccact cactaaaacg agttttgact tttaattctt
     3061 tatgtaagtc tcttgcactt actactggtt cttgattttc gtttagtgtt acgttaatta
     3121 gatgattcat attgtccttt ctagtgttgt acgctttatc tcgttttgag atatttttgt
     3181 ttaaaaaaat aattcactct ctgaaacatt aaatttttga gaaatgatag ccatttcata
     3241 atctttaaaa ggctgtaaac catcttcttt ctcagagtaa ccttttctat tttttaaacc
     3301 aattaaatca gccatataca tttctgtgta ctcacgtttt aaacgttctt ctcttaaacg
     3361 ctttttaggt ttgagatgtt cctgttttaa acgttgtttt ttcgtcattc ccccacctcc
     3421 tttcgctttg cttaattcct taagcttgat tatattatat ctcaaaacga gatattttgt
     3481 caactatttt ttatctcttt ttgagatttt tttatttact tttttgtttc ttgatgttat
     3541 atttatagta gagaaaggaa aaatattatg aacattctcg gaaattctat caaagaaatt
     3601 cgaaaatcca aaaaaatgac ccaaaaagag ttagcaaaac taactggatt taaacaaaat
     3661 acaatttcta atcacgaaaa tggtaataga caacttgacg aagtagatat tcgaaaatat
     3721 gctaaagcac tgaaaataga gccacaacag ctttttgact attcctcttc tcccacaaac
     3781 ccccaagttg aactcatccc atctactcta caaaaaatca cttctacttc ttctcaatta
     3841 gaacacaagc gacaactaaa cgttcttgat tatgccgaaa cacaattaga acaacaaaac
     3901 acagtagaaa gcaataagga tatagtagaa gaaccacaag ccacctacta cacctacaat
     3961 tactacgacc acgcagcttc agctggtaca ggtcagtatc taaatgatgt acaagtagaa
     4021 acaattgaat taccagtcga ttatgacgct gattttgtca taccggttca tggcgattct
     4081 atggaaccaa agtatcactc tggggactat gtgttcgtta agctatccgt agagctcgca
     4141 gatggcgata taggcgtctt tgaatactat ggtgacgctt atatcaaaca gctacttata
     4201 aatgacgagg gggcatttct gcacagttta aatagcaagt atgaagatat acccgtagat
     4261 agagatagcg actttagaat tattggtgaa gtcgttggaa gttattcggg aaatcatagc
     4321 tgatgagtga tagttaaaga atttaggtta ggagattgat atgaagtttt ggaatttggt
     4381 agttaaaact ctaaaacaga aaaataatca ggctgtagct gaaaaggttg taaaaattac
     4441 cgaatcgact actaatataa agtcaccaag ctatcccgac ttaaataagt atagagtaaa
     4501 tccaagcggg aaaagatatg atgatacata cataacaggt gtaggataca aactacgtga
     4561 gattttattg ttggtgtggt gggggcgtac taaaaacccc agaaaaccga caagtaaacc
     4621 accacgctac ttcttctacg attatcattt aaatactaaa aaaactacag atatgtttat
     4681 tcgagatgga ttgcttaaaa agaataaaga agggtgtatt actttaacac tatcggggaa
     4741 agttctttac gatgagtaca aaatcctctg ggaaatacat tcgtataaag gatatatcgg
     4801 tgaattacca aacatggata gagtgttcca cggatggaat tacaactctt ataaggccaa
     4861 taacaattta ttagagatta gacatttaga agacattgta aaatacaata caattatgag
     4921 agatcaatat aaaaaaggta gcaatgaata taatgcgttt caacaagata ttgagcaaga
     4981 taaaaaccag atagcactat tgttcaacga acatcaattg cttgaaaata tcgataatta
     5041 gttaatctaa aacaaaaaac cccacgctct caaagtttgg cgactctgag cgtgaggcaa
     5101 gacagtatag taaaaacctg cttcgcagta ggtctcttta ctatacctat tttaacagaa
     5161 aatgaggtaa aaaacaatgt ggtcagaaaa acacaaaagc ggaaaagtaa attttgtaga
     5221 acggtacaaa cacccgtata ctggcaagtg gtgcagagct tctgtcctta tggaaaaaga
     5281 cacgccacga atacgaaaag aagccaaaaa atatcttgac gaaaaaatat ctaaaatctt
     5341 aacaagtctt accacaaccg atgcctacct actagatgtc atgaacgaat ggtgggaaca
     5401 tcatcagaaa tcactcaaat caacttctat ccgctctctt gattttagaa taagagaatt
     5461 gcgaaactta attgatccag aagtgatgat tgccaagata accacgaaat acctgcaaag
     5521 tattatcgac aaaataccag gtagttatga caaacgtaaa cgagcacggc agctgcttaa
     5581 acaaaccttc gactacgcta tcgctttaga atatgtctca atcaatccgg ttattagtac
     5641 ccaactcgca aaacctgtta agaccatcaa agactttgaa gacgttgccc agaagttttt
     5701 agaaaaagat gaactcaaaa ggctattaga tgaaatgtat agacgtaaag gatcgattaa
     5761 aatggcttat ctggcagaat ttatgagctt aaacggctgc cgcattggag aagctctagc
     5821 tatacagcca gacaatatca aaaatgatat cattgaaatc cacggaactc ttgactacac
     5881 ctcaaatggc tatagaaacg ctatcaaaac cacccctaaa acaaattcca gttggcgtga
     5941 aacattgata accaaacgtg aaaaagaaat tattcaggat atactgaaaa ttaatgctct
     6001 tgaaaaaaat actaatccta attacaagga catgggctac atcttcattt ccagaaatgg
     6061 cgttcctatt caagacaacg ccctcaacac ctctatcaga gcggcaaata agagactaga
     6121 aaaaccgatt caaaaagaac tcaccagcca catctttcgt cataccctgg tgagtcgcct
     6181 tgcagaaaac aaagtgccct taaaaactat catggataga gttggacatg ctgactcgaa
     6241 aactactcaa caaatctata cccacgtcac gaaaagcatg aaaaatgaag tcgtcgacat
     6301 cttaaaccgg ctataatata ccccaaatat gccccaaaaa tgccccaaaa cacaagaaaa
     6361 aaccttgata tcttcaacaa acgttgatat gacaaggttc tttaatgttt tatttaatca
     6421 cttcttgagt tttagcatac ttggcttcta cagcatcttt ttctgctttc caccatgtct
     6481 cattttctgt ataccactta atagtttctt ccaaaccttc tgaaaagttt gtaaattgtg
     6541 gttcccagcc gagttcttca cgcaattttg tagaatcaat agcgtaacga agatcgtgac
     6601 cagcacggtc agttacgtga tcataagcgt cttttggttg acccattttc tcaaggataa
     6661 gctcaagaac ttccttgttg tttttctcgc cgtcggcacc aataaggtat gtttcaccga
     6721 tacgaccctt agtcaaaata gcccatactc ctatagaatg atcattagta tgaatccagt
     6781 cacggacatt tttcccttca ccgtatagtt ttggcttgat gcctgataaa atattggtga
     6841 tttggcgagg aataaatttc tcaatgtgct ggtaaggtcc gtagttattg gaacaatttg
     6901 aaatggtcgc tttcacaccg aaggaacgta cccatgcctt aacgataagg tcagaagctg
     6961 ccttagttga tgagtaaggt gatgatggat tgtattttgt ttcagcagtg aatttttcac
     7021 ctggtccttc accctgtcct ggaaggtctt cacgaagtgg aagatctcca taaacttcat
     7081 cagttgacac gtggtggaaa cggatatcgt atttacgagc tgcttcaagc aaagtgtaag
     7141 ttccgataaa gtttgtatgg ataaatggac ttggatcttc caatgagtta tcgttgtgac
     7201 tctcagccgc atagtgaaca atagcatccg ttttggcagc caatttgtct accaattcag
     7261 cgtcagcgat atcaccaaca actaactcaa cacgatcacc aagaatagct tcaatgttag
     7321 cacggttacc tgcatatgtc aatttatcaa ggacagttac atgaacatct gggtggttat
     7381 tgtagacata gtgcacaaag ttagatccga tgaaaccagc tccaccagtt acgataatat
     7441 ttttatacat aagatataaa gggacctttc gtttccgaaa aaatctatta aaagtaataa
     7501 ctctaacaaa gacgttttag gcctaagaaa acatcgcttt ctaagatttg agtcccatgt
     7561 tcagctcaaa gagagaaact gaactttcct ttcatcattt ttagttgtta cttgttacct
     7621 gaagtagaga agaataactc ccctcatatc cacctcaaat gattaatccc aatcatctga
     7681 gtatgttgtt tccctatagg tcttttggtt tcagtggttt gacatcacta agaagaggat
     7741 ggtttttatc tgcttcagaa acttctgcag ctgctagatt ttcccaagta atcccaagac
     7801 ttgggtcagc atagtttaca aaagcatact taggtttcaa gtcaagagcc cagtagtcat
     7861 tgacaagata actgtaagag acagtctctg aaagaacttg aaagccatta gcaacccctc
     7921 taggaacaaa cattcctttt gaagcatcaa tcactgtctg gtagacgttt ccaaaagtct
     7981 ctccctcacg aagatcaacc caagctccta aaaccttgcc atcatcagca actgagatgt
     8041 atttatccca aggttcagca tgaagtccac gcagcacatg ctgacgtgaa aaagaaacgt
     8101 tgttttgtag ttttccttcc tcaaaaaaac gttctggaaa gccaattggc aacatttttt
     8161 ctttttggaa attttcttta aaccagccac gattatcccc acgaacagga atatcaaact
     8221 ctagtaagcc tgggatttct ttgatttctc ggcaagccaa tggtttgtca aaaaaagttt
     8281 ctgtcattaa gcttctccaa tcaaacgaag caagtattgc ccgtattcgt ttttctttaa
     8341 agactgcgcc aattcatgga catcttcttt actgatgtaa cccatgcgat aggcaatttc
     8401 ttccagattt gccacttgag cgttttgcaa acgttgaact gtttcaatat attgagccgc
     8461 ttcaagtaga ctttcatgcg ttccagtgtc tagccatgca aaaccacgcc ccatcaattc
     8521 aacggagagg tcaccacgtt ccaagtaagc cttgttgacg tctgtaattt ccaactcacc
     8581 acgcgcgctt ggtttaatgt ttttagcaat ttctaccacg tcattgtcat agaaataaag
     8641 tccagtcact gcaaagtggg acttaggcac ttctggtttt tcttcgatag agatagcatt
     8701 catgttctca tcaaattcca ccactccaaa gcgctcaggg tctttcactt gataaccaaa
     8761 aacggtggct cctttttctt tggcagctgc cttttgaagc attttagtga ggccattacc
     8821 gtggtaaata ttatctccta aaataagagc aacacggtca tcaccgataa actcctctcc
     8881 aataatgaaa gcttgtgcta aaccatctgg gcttggttgt tccttatagg ataagctaat
     8941 gccaaattct gacccatctc ctagcaattc ttcgaaacga ggaagatctt gggaagttga
     9001 aatgattaaa atatctttaa tccctgccaa catcaaggtt gacaatgggt aataaatcat
     9061 tggtttgtca taaatgggca tcaactgttt agatgcggca cgggtcagtg gatacaagcg
     9121 tgtccctgaa cctcctgcaa gaataatacc tttcatagta gtgctcctat tcttctatat
     9181 accctatcat tctatcattt tttgaaagca gtgtcacgct ttaccattag accgcaaaag
     9241 gtaaggctca ggggaatagt cgctaggcgt taaaagtcct tcgtgtccta gcaataattg
     9301 agctaattca tagccaatca aaggtccaac tgttaatccc gatgacccta aaccactagc
     9361 tgtataaaag ttcttcaatc ctggaacctg accataaaaa ggagaataat cactggtata
     9421 agctcttatt cctacacgac acgttttttg agtagcttct tttaataacg gcaaataagt
     9481 aagagcttgc atttcaagtt tttctaaaac ctgccaatca ggctccaaat catagccttt
     9541 atcattttcg tgactcgcac caacagaaat cttaccttga ttgaaaggaa taagatctat
     9601 ttcaccttct ggcataacaa ctggataatt gtcgctgatg atgtgatgaa cgtcataatc
     9661 caagagttgt cccttttgtg gtcgcacatc aacctgatag cctaaaggcc tcaataagtc
     9721 aggtagccat gctcctgctg ccagaatgac ctgatcaaaa taatgaccag caatctcata
     9781 accacttcct tgctgtttca gagtgacttt ttggcgaata actggataac cactagcctt
     9841 gagtaacgtc tcacataaag tagccccttc aactctagca gctcctgaag cgtacagaca
     9901 gttatcaaag cctatcaatc ctttaaaatc attccccagt tctttacgat ttttaatggc
     9961 taactcacca ataatcggtg attctacctt tcttgctaac gctaattcat aaagatctct
    10021 tagtttttct tcctgctttt ttaaaacata gatgccattt tgctgataaa aatctgttgc
    10081 aaaaccatct tctttgaggt cattgattaa ttgctgataa aagtccgctc ctaaacgagc
    10141 catgcggtac caaactttat tgcgacgctt tgaaaaccaa ggactaataa taccagcagc
    10201 agccttagtg gcctgtcctt gaccatggtc aaaaatggta acttcttttt gaccactttg
    10261 ttgtaaataa taagcggcgg ttgatcctac aatccccgcc ccaataatgg ctattttcat
    10321 tcctggtatc cttttatttt aagaccttaa cagcctataa atggctaaat ggattggtcg
    10381 aggctttgct tgaaataatg ctgacatccc aaccgttctc ttctttccac ccttggagtt
    10441 tctcctttaa tttctccgta aaaagcacct caatatgatg ccccgggtca accgcaaata
    10501 gtccttctgt cagcatctcc tgtgctgtat ggtaatagat atcccctgta atataaacgt
    10561 ctgcgccttt ttgcacagca tcctgataaa attcaccacc acttccgcca cagatagcaa
    10621 tctttgaaat caaagggttt tccttatcat aacggataag tcgcacggta tctaaatcaa
    10681 acaccctttt gaccttactt gctaactctt ctaacgcttg ttctttaact gttccaatac
    10741 gaccaatgcc aaagccttct ttggtttcag acaagtaagt tgcttccttg atttctagca
    10801 ggtcacaaaa ccaatcattt agcccacctg gtacaatgtc aatattggta tggctcacat
    10861 agacagaaat gtcatgcttg accaaatcta gaaggatatc acgctgaggg gatgaaacca
    10921 gatctttaag cggtttaaag atgggagcat gcttagttat aatgagatca acttcatttt
    10981 tgatggcttc tgctactgtt gactctcgga tatctaaagt aatcataacc ttacgaatgt
    11041 ctttatccaa tgaacccatt tgaagacctt taacgtctcc ttccatagat aaatcaagtg
    11101 ggcaaaaggc ttcataggca tcaattaggg ttttagcttt catgaacaat cacctctttt
    11161 atttgttgta tttttgtcaa gagaagctga cgctcttgag ttttttcttc tgggatgcag
    11221 gacaaggcat aagctaactt atccatttct cgttgccact tctctttaaa aacgactgat
    11281 ttttcctgag aaaggtatgg accaaaacgc aattctgtcg cggataaggc cttttcacca
    11341 tgctcagcaa cgatgatttc ataatatttg tcattttcag ccatgatggt ttctgcaaca
    11401 attttaaaag cgttaaccga taaccaagcc cgtaaatcat cctcacgatt gttgggttgt
    11461 aaaaccagac gttcaatgcc ctgcaatttt tccttacctg cctctaaaat atcagcaatc
    11521 aaacggccac ccatgccaca aatggtaatg gctgtcacat catcagcttc ttcaaaggct
    11581 gcaaggccat tggctaagcg cacctgaata tgctcggcaa ggccagactg tgtgacattt
    11641 ttaagagcag attcgtaggg acccctaacc acttcgccag caatggctgc tgaaatctga
    11701 ttagtttcta ccaaaaatat gggaagatag gcatgatcac tgccaacatc taataatttg
    11761 acaccttttg gaacataggc cgcaacttgg gccaaacggt ttgataattg actatccatc
    11821 ttgatttctt tctaaaacgt aatgattcta ttatagcaaa attacctcca taaaaaaaga
    11881 aaaccaaagg ctaggctttc gattttccat aagtattttg atagtacttg cagtaagact
    11941 gaacgggaca aatctcacat tttggttttt tggctaaaca atggtagcgt ccaaagaaaa
    12001 tcaatcgatg gtgagtaata atccaatctt tctttggaat tttagccatc aaatctgctt
    12061 caatctgctt gacatcggca tctggtgatg aaatattgag cctctttgag actctagcca
    12121 catgagtatc aaccgcaata gctggaacac cataaacctc tgccaatacc acattggctg
    12181 tttttcggcc aacacccggt aaactttcaa gttctttgtg ggtttttggc acttgaccct
    12241 taaaatcatc acgaatagcc tgagcagttt taatgatatt tttggcctta tttttataga
    12301 gtccaatcgt tctcagagca ttttcaacat cagaaagttc agcaaaagct aagtcttcta
    12361 tttctggata agactgccat agtccaggag ttaccttatt gacggcctta tctgtcgttt
    12421 gagctgataa aatcaccgca atcaacaatt gaaaaggtgt ttcccagtct aactctccct
    12481 tagcctcagg aaacatttga ccaatgatgg tcaggacttt tgctaatctt gcttttccaa
    12541 ttctcatgaa tcactccata aattcatcgc agatagaaaa tcctctgata tcgtcacttg
    12601 agaaagatct tcgccttctc taacacgtct acgttcttct acttgtcgaa gattaacaat
    12661 accttctttt cgccaattac gtaagatagc ctggatatat ttccaatttg ttttgccgtt
    12721 gaagacagct tcttttaaag cttctcgtat caaatcagga tctgttttat catcacgaag
    12781 cgttttttca agatcttcta actcaaaagg actcaagaat cgtcctaatt cacgttcaaa
    12841 ttcatcaact aatcgtttga aatgatttgg tgtttcttgt ttgtcaattt cagttgccgt
    12901 ttgactgaca aacaattgat ctaacttggc taacacagga ctcgcatcaa aaataatttc
    12961 aatttctcca gtaagttcaa ttgtccgcat atctaaaagc ccttgactag tcaaagatga
    13021 aatgatttta ttaatatcag caactgattt tccaagcgca tgagcaattt gactgggcgc
    13081 taagtcgtct cgctttgtcg tattttgaag atagaaaaac tgccagacca aaaaatcatc
    13141 tgagctctta aaaagatctt tgtaatgaaa aagcaaggca cttgggataa ccaaattacc
    13201 tgacttataa tgttctaaaa aactcataat ccctcaatca aaaggctaaa atcgaatcta
    13261 tagcctttac tactttaaat cccctaatca gatgctgatt aatgtgctta cccaggaaac
    13321 tgcattaaga ctttatagtc atagttccta atagcatgac gaccatttaa accttctaat
    13381 tcaataagga aggcacaacc agctacaata ccaccaagtt tttcaatcat ttcaatagtt
    13441 gcttttaccg taccgccagt cgctagtaaa tcatcgacaa tcaaaacacg ttgtccaggc
    13501 ttaatagcat ctgcatgcat agttaaagta tcaagaccat attctttttc gtaatcggca
    13561 gagacaacat ctcttggcag ttttcctggt ttacgaacgg gagcaaaacc aattcctaac
    13621 tcaactgcaa caggacaacc aatgataaaa ccacgcgctt caggtccaac aaccatatca
    13681 atatctttat cacaagcgta ctgtgctatt tcacgaatag catagctata tgctttaccg
    13741 tcagccatta aaggtgagat atctcgaaac gtaataccag ctttagggta atctttgatc
    13801 gatgcaatgt aatttgttaa atccatagtt aattctttct agtggcaaat tgccaaatat
    13861 aaaatactct tcattatatc acgaaacata tttttcgtaa aataagactt ctgacattat
    13921 tgtttatctg atacttcatt cgtaactacc tcaataacta ctgtcaactg ctttacttat
    13981 tgatcgtttg atttcaccaa ataatcatac atctctttcg ggctagccaa agccatgatt
    14041 tcttgaaatt tcaccaattc ttttaagtct tggtaaatgt ggctctctga aatctctcgt
    14101 ttttgcgctt gggggttaac cgtcatcagc ccatcgtcaa tcgtcacaaa agacaactct
    14161 tcaaaaattt gaatcaactt aatcagtaaa agtttttcta tgtttaaata gtgactcaac
    14221 tctgtcaatt tatgccgtaa atcaaattca ggaaactggt agatggtttt gtagagcttg
    14281 gcaaactgct ctctgctgcc aaatcctgtt aaataataag gatgtttcat ctgattttta
    14341 aaatagatgg cgtcaaaatc cttgtgaaca aactggttgc gccaggtttt gaaatcttca
    14401 gggatgtcat taatcagaat gactctggca tttgggtctt cttcaatagt cggaatacct
    14461 tcaggaactt tggctgtttt tgtccttaag tcaagaagtt gcactccgtc aacacgagca
    14521 tctactaaca tgaattggag actcgtgttc ccattccaat ggttgacaga taaggtaact
    14581 gctaattcta aaccagtagc ttgtcggaac tcttgcagtt gacttccttg accaaaagcc
    14641 agtacgtcaa aggaagcttt gccctgactg actttaaatt taagatggct ctggtcttgc
    14701 cctatggtac gcgcttgact aacccttatg tccttgacat aaaagaccgg cttttgatga
    14761 tccatcccat aaggggctaa tttatctaaa ctctttaaaa tatcaaggct aaggtcgtcc
    14821 aaagacagtc tttcatcaat agttaacgta tttttggcag tctgatctaa gccacgttct
    14881 atcacaaact gacaaagaaa gtctgaaaga gcttccaagt tgtcaacggg cagggtcatt
    14941 cctgctgcac cagcatgtcc cccaaaggct gtaaataact cacgcttgcc attcaaggct
    15001 tcaaaaatat taatagcttc taaacttctg gccgagcctt ttgcaaagcc attatcaatg
    15061 gtcaaaacga cgaccgtttg tccaatagtt tccatgatac gaccagcgac aatcccaaga
    15121 acaccaggat gccagccagc ttgtgccaag acttgaacag gtttttgagg atcaaccata
    15181 gccatcgctt gatcaaaaat gtcttggact aaggccttac gctcttcatt ttttttctta
    15241 atcatcaagg caatcgcttg agcttcttgg tcatcaaagc ccgtcagtag ctcaatggct
    15301 ggattagggt catctaagcg ccctaaagca tttaattgcg gagcaatctg aaagccaatg
    15361 gcgtcttcat taaaatgttc caaatcaata ggagacaagg acatcaactc ctgcaagccc
    15421 actctttcgg tgtgtttgag catggccaga ccatttttga ctaaaacacg gttttcacct
    15481 gtcaagctta ccatatcggc aatggttcca atagctacca agtctagaca gtctgttggt
    15541 aaactttcta gcaaagctgt tgccaattta aaggcaacgc cacagccagc taagtgttta
    15601 aagggataat cagcatcagg atgctcagga tgaatgatgg caaaggcttc tggcaattct
    15661 tcaggcaggc tatgatggtc agttacaatg acatcaactt cctgttcttg agcatatcta
    15721 atcgcttcat gtccggccac gccattatca accgtgataa ttagggacac agcttcctgc
    15781 tcaataaaat atttatagac gctctggttg gggccatagc catctgtaaa gcggttggga
    15841 agataaacca aaggctctgc tcccatcatg tctagggttt ctttgacaat agaagccgac
    15901 gtcatgccat cagcatcata gtcgccgtaa atgagaattc gttcgccctc ttcaatagct
    15961 tgacgaattc ttggaacagc ttttgccatg tcatgtagca agtaaggatc atgcagttga
    16021 gacaagtccg ctgttaaaaa ctcatctaag gcttcttcag tccttatccc tcgatcataa
    16081 atcaactgag cggcggtctg ggttagacct tttgtctttg ctagcttaaa gaagccatca
    16141 tctggcttct tgtcttttat tttccagcta tatttggatt taatcattta taattaaata
    16201 cctttcttgc aagataatca gataatttag ggaaaagggt gtaaaattga tgggtcaccg
    16261 ctaaactaaa gggcaaattc aattctcgtt tatttttccc gataatagaa accaaacgct
    16321 tagccacttg atttggttgg agagtaaatg taccaacgct ttccaaataa tgtccagacg
    16381 ggtcagcttg gtcaaaaaac ttggtggcaa tgggacctgg attcacggtg gtcacgtaaa
    16441 cccctttatc cgctaattct aagcgaaggg cattggaaaa tccgataagg gcaaacttgg
    16501 tggctgaata aatgctcgat ttggctgacg caatcaagcc tgccatggac acaatattaa
    16561 taaggtgacc ttgcccctgc tctgccattt tctgaccaat caagcaagca aagtgaatgc
    16621 tcgctagggt gttaacctga aacatatcag ctatttcttg ggcagaaaac tcttcaaagc
    16681 ctttgaaagc tccgtagcca gcattattaa tcaagacatc aatacggcca tagcgctggt
    16741 aaatctgggc gaccattttc tcaatggcta ctggattggt aatatccaac tcaaggcatt
    16801 ctttgttgtc aatatgctga taacagtgtt ctaggcgttc tttgttacgt cctaataaaa
    16861 tcaagctgtc ttccttgggt aactgcttaa caattgcctg agccagtcct ccagaagctc
    16921 ccgtgataac aatgattctt tgtgccatta aataatcact tcctctaaat cttgtaccat
    16981 tttaacattt tcaaatatag tggcggcatc tttttccatc tggcgacaat cacgccctaa
    17041 aaagcgagct gatacatgat tgagcaagag gcgttttgcg cctgcttcat gagcgatttg
    17101 tgctgcctgc atgttggtag aatgcccgtg atttctcgca atccgctcgt cacccttacc
    17161 ataggtggat tcatgaacca aaacatctgc atctttagcg agtttaacac tggcagacgt
    17221 tttgcgcgta tcgccgataa tggtgatgat tttgcctttt ttaggggcag aaatataatc
    17281 tttggcacaa atcaggcggc cgtcttccaa ctcaacgtct tgaccatttt taattttgcc
    17341 aaagagtggt ccaaaaggaa cacccgctgc ttttaaggct tctgcgtcta gggttccttc
    17401 taagtctttt tgaactactc gataacccat gcaaaaaatg gtatgcgcca gacgctctgc
    17461 atagacctca aacttatctg tttccattat tttacctaat gatttgtcat caaattcatg
    17521 aaaatgaatc tgataaggta cacgcgctcc agaaactttt agactagtta acacgtaagt
    17581 tttaatgcca attggtccat aaatatcaag atctgtctgt tcttcactag cttgaaaaga
    17641 ccgacttgat aaaaatcctg gtaatccaaa aatgtggtcc ccatgcaaat gagtaatgaa
    17701 aatctttctt atttttcgtg gcttaatcgt cgtttctaaa atttgtcttt gcgttccttc
    17761 accgcaatca aacatccaga cttcattgat ttcgtctagt aatttcaagg caagacttga
    17821 cacattgcgc tgcttggctg gttgaccagc tccagttcct aaaaattgta attccattca
    17881 atctattctt tctattgggt gatgtaaatc ataaattctc gttgggcaaa atcataatac
    17941 ttttgaccag aaaatgtttg gctaagagcc cataactcct ctacattaaa ccccttaagc
    18001 atggtgatac cgtttgccag acggtaaatc ttcgacaaga gtgtgtcatc aggcgtaatg
    18061 tcttcacatt ctccttgctc aaactgctta gcctgcattt taagctggcc tttgtcttca
    18121 taaaccatat attgtgggaa aatttgtgtt aattcaaaga agagttcggg tttcttaggc
    18181 tctgcttttt cagaaaatac caatgcaact tcttggtcat tttggtaagc aaaaccataa
    18241 gcttttccag acaaattgag gcgtacaaag ggcttttctt cctcaaaaga tggttggtca
    18301 gtaaaaagcc ccaaagcctt agaaagatta aaataataat cagtcgcagc ttgcggtgac
    18361 tgtttttggt agatttcagc aaagtaggca ttaataacac ttggtggtgg tgtgataaaa
    18421 gctagttttt gctggtctgt caaactggct aatagatggt ttaggtagtt ggtatccaga
    18481 ctagtaaatc caccataggt ttttgctaaa tcaatataat tattcataaa aatcgtcaag
    18541 tctccattgc tgttttgggg aaatgtagcc cgaaatatcc tctatttctt ggtcaaacgt
    18601 gtagtggtct aaaagagcaa ctcgattcaa atcatacagc ttgtacgcct tgtcttgatg
    18661 cactttgatt ctaaaaggaa caaactggtc tcgaatttgg tcaatgatta aacgtctcaa
    18721 gagaattttg ctatcctttg aacgggctga aatcctgata tttggaaaag cagtagctgt
    18781 gaattgttct gctatatcca ccttgttata aatagctaaa cgcggaatat taagcatgtc
    18841 tagctctttt agaaggttta aaaccacttt ttcttgctca ctatgattgg ggtcgctagc
    18901 gtcaatcaca tgtaacagca aatccacata tttgctttct tctagggttg atttaaaagc
    18961 agccactaat tccgttggca agtcttgaat aaagcctacg gtgtctgtta aggttgcttg
    19021 aaattgattt tctaaataga gttgctttgt agttgcatct aaggtagcaa ataattcatt
    19081 ggcttcatag tggctgtcat ctgtcaacaa gttcataatg gttgacttgc cagcattggt
    19141 gtagccaatc aagccaattt taaaggtatc tgaacccaca cgacgttctc taattgtttg
    19201 gcgatttttc tctacctgag ttaattggcg ttcaatatca gcaatctgat gacgaatcga
    19261 acggcgattt aactctagtt ggctttcacc tggtccacgg ctcccaatac cacctgcttg
    19321 acgacttaac atgatgccct gtccaaccaa acgaggtaac atatatttca attgggcaag
    19381 gtgtacttgt aatttgccct catggcttct ggcacgcatg gcaaaaatat ctaagattaa
    19441 ctgcatacgg tcaatgactt ttacctctaa aacagcttct agattagcat tttgcctagc
    19501 agtcaaccga ttgttcacaa taactgcatc aatctcatca gcttccacaa tcgccttaat
    19561 ctcatccagt ttccctgagc cgataaaggt tttgctgtcg taacgttctc gtttttggct
    19621 gaaacttgcc ataactttga caccagctgt tttggcaaga ttagctaatt ctgtcatgga
    19681 catgtcaaaa tgttctgttg tctgaagctc aactcctaaa agaatcactc tttcttgctg
    19741 acgctttgtc tcaatcatca cctttaccaa tcctccaaat tccgtaaatt ctattataac
    19801 atatcttcaa cagattctgc taattttgcg tcatgacttc tctttctgac caagaaaatc
    19861 cctgacccta tcatgtacta cctgtgggta atcaggagcc gttatggcag tgaagctaac
    19921 agccattcga ttgcggaacc aagttagctg tcgcttggca aaacgtctcg tgttttgttt
    19981 taattgatca ctagcttctg ccaaagtcat ttcacctaca aaatagggga ataattcctt
    20041 ataacctatg ccacgactag cctgaacagt cgggtaatgc tcatataacc attttgcttc
    20101 ttccaataag ccgttttcaa gcatacgatt cacgcgctga ttgatacggt catagatgac
    20161 ttggcggtca tcattcaagc cgataataag cggctcataa gccgtctcgg cattctctaa
    20221 ttcatcagca aactgagcca attcaagggc tcggatcgcc cgtctgcgat tcacctgctc
    20281 aatggtaatg ttattgactt gcaagcgttc atacaagtcg tgatcgtcca attgctccaa
    20341 ctcatttcga taggctttta ccgcctcttg atcaacttga cctcctagat gataaccttc
    20401 gagtaaactt tgtaagtaaa gaccagttcc acctacaata attggtaatt tcccacgact
    20461 aacaatgtct gaaatagctt tttgagcatc ttggacaaaa tcgtaggccg aataagactc
    20521 cgtcacctca cgaatatcaa ttaagtgatg aaccgctgct tcttgctctt cttgagtggc
    20581 cttggctgtt ccaatatcca actgtcgata aacttgctgg ctatctcctg aaataatttc
    20641 tccattaaaa gccttggcta aactaatccc aagtgctgtt tttccaaccg ctgtcggacc
    20701 aacaatcaca actattttta tttttgtcat tttcttgtct ttctattaga aaagcgtgtc
    20761 caaaaacgcc ttttacttga aaattagctt atttttcgct accatatatt ataacataag
    20821 attaaggacc actgaggtcc ttcactagaa atggagaata atcatggtta agaaatatca
    20881 atttgttaaa ggtcttgcta ctggcgtcct agcaactgct gctactgttg ctggtgctgt
    20941 ctttgctgta aaaaaaacaa tcattgaccc tgaagaagaa aaagctgctt tcattgaaga
    21001 aaaccgcaaa aaagctgcgc gtcgtcgtgt agctcgctaa cgtgacaatg gatcaattta
    21061 aaagagacta gcacattaaa gtgccggcct cttttttagt aatctcgata actagttagt
    21121 agtaactgtc ttttgatgag ccaagatatt agctgcttta cttattttga aatagaaatc
    21181 tcaggtaagc cccataaaca taagtgacta aagctgtcgc caacaatacc tctcctagca
    21241 aaagaacctg ccaacctaaa ccttgccaac ctaaaaaaga taaggtcatt tttaaggaag
    21301 tggcactaat cacaaacgga aaggtaaaag ctgaaaaacc tgggttaaag ggacgattga
    21361 gtaagcgagg aagctgtaca accacaaaag catataacag ttgtgagctg atgagaagaa
    21421 agataaccat tcctacttgt ggtctaggaa aggttgctaa ataagacgct gaaagtaaag
    21481 acagaggggc acaaaacgtt gaaatattag gcattactgc ttgacctaga ccaatcccat
    21541 aagtcttttt ggccataaaa ggtaaaatca cagcagttag gggaagacag acccaaaaaa
    21601 tcacctgtcc taataaaaac tgcctactag caggtgctgt taatgccgcc attgcaattc
    21661 caacaaaaag cacggaccag gaaggaaaaa cgttatccca tgaaaaagaa aagacaaaac
    21721 gatactggta ataagcaatc aggacaagat ttcccaaaaa gaatagccac caagtgagaa
    21781 agcctaacca acaccctcct gtcactttga gaaagagtga agacaacaac acccctgtca
    21841 taaaaaaagt tggaaaaaca gaggcaatta gaggttgtcg taactgcatt ttggtatcgt
    21901 taagattcct aaccatgcca acaagcaaga taccatagat aaaaagagct gccagaattc
    21961 ccaaataact aaaaatcgaa acataagtag ccaacaaatt gccaaatgac aacgttccta
    22021 atgccaatcc tgacatgacc aatggtgggt ttttaaggtg tttcataagt gtccttttct
    22081 gcctattaac agctattttt agtgacataa tcttgtttat tttatcacat ttcgagcatt
    22141 tttgccataa aggctgccag atgcggcacg ccgtacgcat gaaaactttc tcttcatcct
    22201 tttgtgcccg gacatcaatt ttgaaaagat tcctttttaa atcaatcatc tgcttagcct
    22261 ctttctccaa ataattg
//
LOCUS       DAUGHY010000030        21803 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.030, whole genome shotgun sequence.
ACCESSION   DAUGHY010000030 DAUGHY010000000
VERSION     DAUGHY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21803
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.030"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            6..1301
                     /locus_tag="VH752_001126"
     CDS             6..1301
                     /locus_tag="VH752_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8289488.1"
                     /translation="MTKIYIFSFLSDITLPVWKQLTILALALFFNQFPYLSPLLIDPL
                     LFLVVLRQETKQLFSLKALFLAVAPSVLVDLLSRFMGTIVIPYLFLSSGIYLGHIIFD
                     LLAYLLIFPSFAIINYMIGKDYKMICQSGYSKRSHNFYQTLLIFVLVYYVDIFVILGF
                     TDPFLHFHHSLFVPTPYKLLFLMFILLLVYLLSYFNHSSKEYLKNELRREQQAYMTNL
                     ETYGKHLEKLYRDVRAFQSDYLSRIERLGQAIKSESITQIQDIYAQTVHEANDYWDDK
                     HYNISKLRKINISSIKSLLSAKIISAEKSGIDLNVEVPDNIKETYIPELDLLLLMSIF
                     CDNAIEAALEAQQPHMSIAYFLLDDYQMFVVTNTTKKKVDINKIFEEGYSSKGSERGI
                     GLSNAQRILKKYPYLSLRTKSFDKEFSQTLTMPKEEVDR"
     gene            1298..2581
                     /locus_tag="VH752_001127"
     CDS             1298..2581
                     /locus_tag="VH752_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8289489.1"
                     /translation="MMVIYSMALFYATIFINSWVIFAKVSAIKLSWKRVSVIGISFVI
                     ANMIFDKVILIDQLFFIIVSLLSAPKKKLFEHMFNGFFTILIVELLFRVIGSFFLPAV
                     LGFSIGQINNNLKLLELCYLFVLPIFYLFSYIFSIDLSLIRFISEDKMKKWVFWMNTA
                     MFSYYFFAHFLVTVQSGFLALYFQYRSILVFIYLAIFIWVIVKLDRFAKDQLSQKLTQ
                     AQNERIAYLENYNQSIEQLYREIRTVKHDSENILISLKDSIDSGDIDLITRVYDTVIQ
                     QSATSMMRTNYEISSLDNIKEAVIRSIMNSKLLEAQYLGIELYIEIPDVIDHLPIKLI
                     DLIVLFTGLVDNAIETAKGSRRPFLSIAYFKQDNKQLFIIENSTKTNRVDIAKRFDAQ
                     QQNSAHFLTVLDSYPQITLSTKSDHYRLRQLLEMR"
     gene            2585..3325
                     /locus_tag="VH752_001128"
     CDS             2585..3325
                     /locus_tag="VH752_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8289490.1"
                     /translation="MMNIFILEDDFIQQTRIESIVVGILKETRIPCNQLEVFSTPQKL
                     FESIQERGDHQLYFLDIEIGEYTRCGLELAAAIRQKDPNAVIVFVTTHSEFAPISFKY
                     KVSALDFIDKAGGQKQFKEQIEECIRYTYDMMSSRESKDMFLFETPQTRLKLPYKDIL
                     YFATATTPHKVCLWTQTERLEFYGNLSEIQAVAPKLFLCHRSYLVNLDKVVRIDKSKQ
                     LLYFENGDSCMVSRLKMKCLFERWEALR"
     gene            3865..4224
                     /gene="rnpA"
                     /locus_tag="VH752_001129"
     CDS             3865..4224
                     /gene="rnpA"
                     /locus_tag="VH752_001129"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEQ8289491.1"
                     /translation="MKKTYRVKCEKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGYQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            4190..5017
                     /locus_tag="VH752_001130"
     CDS             4190..5017
                     /locus_tag="VH752_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ8289492.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLMLMPLFNMQIKSSQKMQDIQPELRELQKKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIFFMGFNL
                     ASGVVLYWTVSNAFQVVQLLLLNNPFKIIAERQRLANEEKERRLRERRARKKAMKRK"
     gene            5029..5943
                     /locus_tag="VH752_001131"
     CDS             5029..5943
                     /locus_tag="VH752_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEQ8289493.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLSRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINQKNAPAVNSADVEPEEIKATQKLEAED
                     TKVVPLMSEDSPAQTPSNLAETVTERKVQQPSIPVEESEVPQDPGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKIVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            6257..6391
                     /gene="rpmH"
                     /locus_tag="VH752_001132"
     CDS             6257..6391
                     /gene="rpmH"
                     /locus_tag="VH752_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEQ8289494.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
     gene            6665..7369
                     /locus_tag="VH752_001133"
     CDS             6665..7369
                     /locus_tag="VH752_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEQ8289495.1"
                     /translation="MPDKPTKEKLMEQLKGGIIVSCQALPGEPLYSETGGIMPLLAKA
                     AQEAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMAEVDQLAALNI
                     AVIAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTT
                     LSGYTPYSRQEAGPDVALIEALCKAGIAVIAEGKIHSPEEAKKINDLGVAGIVVGGAI
                     TRPKEIAERFIEALKS"
     gene            7418..8737
                     /locus_tag="VH752_001134"
     CDS             7418..8737
                     /locus_tag="VH752_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ8289496.1"
                     /translation="MNMKKLASLVMLGASVLGLAACGGKSQKEAGASKSDTAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTKEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALNKKMLKDAGVLDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPR
                     AFFANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFA
                     NGQTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDE
                     QKVAASKTFIQFIADDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMEKIAEWTKFYSPY
                     YNTIDGFAEMRTLWFPMVQAISNGDEKPEDALKAFTEKANKTIKKTQ"
     gene            8841..9728
                     /locus_tag="VH752_001135"
     CDS             8841..9728
                     /locus_tag="VH752_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ8289497.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTDFTFVGFANYARMFQDPIFMKSLMNTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIAILLTTSVGQPIILYIAAMGNIDNSLMEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            9741..10571
                     /locus_tag="VH752_001136"
     CDS             9741..10571
                     /locus_tag="VH752_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ8289498.1"
                     /translation="MTKKKLTASDVLTTVILCILTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQWILFSIFIAAMALPKQVVLVPLVRIVNFIGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            10728..11390
                     /locus_tag="VH752_001137"
     CDS             10728..11390
                     /locus_tag="VH752_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEQ8289499.1"
                     /translation="MTSKKQGLLHSLFKLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMALYASLLDWREGQVSQLFTTYSSYFKHYFKRGMRLGLIELLIMSICVLDLF
                     LIRNQSGLVFQGFKVLCIAVLFLVVILFLYAYPQAVRKELSLSTLFKRSFLLAGLFFP
                     WSFAFLALICLTIFSLQLSLLTLFGGVSLLAIIGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            11402..12316
                     /locus_tag="VH752_001138"
     CDS             11402..12316
                     /locus_tag="VH752_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEQ8289500.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGAISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDDHIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIVDKDLEKAKTLQYTINEIIGVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLAELVEEDRVICQRAAALINQAKETF"
     gene            12338..13276
                     /locus_tag="VH752_001139"
     CDS             12338..13276
                     /locus_tag="VH752_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ8289501.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDKGEIFYAGPQIPNYAGTQFKKEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVALAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMAQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITPEKIENNLASS"
     gene            complement(13387..14217)
                     /locus_tag="VH752_001140"
     CDS             complement(13387..14217)
                     /locus_tag="VH752_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEQ8289502.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEKLLNLAKLIDASERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL
                     "
     gene            14502..15293
                     /locus_tag="VH752_001141"
     CDS             14502..15293
                     /locus_tag="VH752_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEQ8289503.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHETEELTLAQEMGVAYHNVV
                     GFDQATISGALALADQYPNVYATIGWHPTEAGSYSEAVEESIVSQLSHPKVIALGEIG
                     LDYYWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEVIKAAGVGPRGGIM
                     HSYSGSLEMAERFIELGMMISFSGVVTFKKALDIQEAAQHLPLDKILVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKETTANAKRVFKLD"
     gene            15265..15855
                     /gene="rnmV"
                     /locus_tag="VH752_001142"
     CDS             15265..15855
                     /gene="rnmV"
                     /locus_tag="VH752_001142"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEQ8289504.1"
                     /translation="MQRGYLSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEAVPSSKS
                     KGRSLGVEHANFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMETYEE"
     gene            15969..16841
                     /gene="rsmA"
                     /locus_tag="VH752_001143"
     CDS             15969..16841
                     /gene="rsmA"
                     /locus_tag="VH752_001143"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEQ8289505.1"
                     /translation="MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVKD
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            17265..18137
                     /gene="rsgA"
                     /locus_tag="VH752_001144"
     CDS             17265..18137
                     /gene="rsgA"
                     /locus_tag="VH752_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEQ8289506.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYDFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALETGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALETGELWPVRYEHYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            18147..18809
                     /locus_tag="VH752_001145"
     CDS             18147..18809
                     /locus_tag="VH752_001145"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEQ8289507.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGADIMTIHTESTRHIHGTL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKGLSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     HD"
     gene            18802..19434
                     /locus_tag="VH752_001146"
     CDS             18802..19434
                     /locus_tag="VH752_001146"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEQ8289508.1"
                     /translation="MTKVALFSGGDLSYFTREFDYFVGIDSGSLFLLENGLPLNMAVG
                     DFDSVSQEAFTDIKEKAELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            19436..20707
                     /locus_tag="VH752_001147"
     CDS             19436..20707
                     /locus_tag="VH752_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEQ8289509.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     MTYQLDTANKQQLLELTQLMNRQQADLYQQLTDIRDVLHRSLSDSRDRSDKRLEKINQ
                     QVNQSLKNMQESNEKRLEEMRQIVEEKLEETLKNRLHASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNAAFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQATKSLLNMPLLEEENNEN"
     gene            20697..21635
                     /locus_tag="VH752_001148"
     CDS             20697..21635
                     /locus_tag="VH752_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEQ8289510.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVEEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPDNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
BASE COUNT         6489 a         3910 c         4673 g         6731 t
ORIGIN      
        1 attatatgac aaaaatttat attttttcct ttctatctga tatcacatta ccagtttgga
       61 aacaactaac gattttggct ttagccctat ttttcaatca gttcccgtat ttgtcgccat
      121 tgttaattga tcctctatta tttttagttg ttttaaggca agaaaccaag caattatttt
      181 ctttgaaagc tctttttttg gctgttgcac ctagtgtttt ggtggatttg ctatctcgat
      241 ttatgggtac aatcgttatc ccctatcttt ttttgtcgag tggtatttat ctgggtcata
      301 ttatctttga cttgcttgct tatctgctga tttttccaag ttttgctatt attaattata
      361 tgattggaaa agattacaag atgatatgcc aatccggata ttcgaaacga tcgcataatt
      421 tctatcaaac attgctgatt tttgttttgg tttattatgt ggacattttt gtcattttag
      481 gatttacgga ccccttttta cattttcatc attctctttt tgttcccaca ccttataagt
      541 tattgttttt gatgtttata cttcttttag tttatcttct atcttatttt aatcatagtt
      601 ctaaagaata ccttaaaaat gagttgagaa gagaacaaca agcttatatg actaacttag
      661 agacttatgg gaaacatctt gagaaactct atcgagatgt aagagctttt caaagtgatt
      721 acctgagccg tatagagcgt ttaggtcaag ccattaagag tgaatccatt actcagattc
      781 aagatattta tgcccaaacg gttcatgaag ctaacgacta ctgggatgat aaacactata
      841 atatctcgaa gcttaggaaa attaatatct catcgattaa gagcttattg tcagctaaaa
      901 ttatcagtgc ggaaaaatca ggtattgatt tgaatgtgga agtgccagat aatataaaag
      961 agacttacat tcctgagttg gacttactat tgttgatgtc tattttttgt gataacgcca
     1021 ttgaggcggc tcttgaagca cagcaacctc atatgtcaat tgcttacttt ttgttagatg
     1081 actatcagat gtttgttgtg acaaatacga ctaaaaaaaa ggtagacatt aacaagatct
     1141 ttgaagaagg ctattcttca aaaggttcag aacgtggtat cggcctttct aatgcgcagc
     1201 gtattctcaa aaagtatcct tatttatcct tgcggactaa aagttttgat aaggaattta
     1261 gccaaacctt gaccatgcca aaggaggagg tggatagatg atggttatat acagtatggc
     1321 tttattctat gctactatct ttattaacag ctgggtcatt tttgcaaagg tttctgctat
     1381 taaactaagc tggaaacggg ttagcgttat aggcattagt tttgtaattg ctaatatgat
     1441 atttgacaaa gtcattttga ttgaccaact gttttttatt attgttagtt tgttaagtgc
     1501 tccgaaaaag aaattatttg agcatatgtt taatggtttc tttactatct tgattgtcga
     1561 attactgttt cgcgtcatcg gatctttctt tttaccagct gtcttaggat ttagcattgg
     1621 tcaaattaat aataacctta agcttttaga gttatgttat ctttttgtat taccaatatt
     1681 ttatctgttt agctacattt tcagtattga cttaagctta ataaggttta tcagtgaaga
     1741 taagatgaaa aagtgggtat tttggatgaa tactgccatg ttttcttact actttttcgc
     1801 ccattttttg gtaactgtgc aaagcggatt tcttgcacta tatttccagt accggtctat
     1861 tttagtattt atttatttag ctatttttat ttgggttatt gtgaaattag atcgatttgc
     1921 taaagatcag ctgtcacaaa aattaacaca agcacaaaat gaacgtattg cgtatcttga
     1981 aaattacaat caatcgattg agcaacttta ccgagaaatc agaacagtta aacatgattc
     2041 tgaaaatatt ttaattagtt taaaagatag tatcgatagt ggtgatatag atctgattac
     2101 tagagtttat gacacggtta ttcagcaatc ggcaacttct atgatgcgca caaattatga
     2161 aatatcttct ttagacaata tcaaagaggc tgtgattcgc agcatcatga attccaaatt
     2221 gcttgaagct cagtatttgg gtattgaact ttatattgag attccagatg ttatcgatca
     2281 tttacctatt aagttaatcg atttaattgt actttttact ggactagtgg acaatgctat
     2341 tgagacagcc aaaggcagta gacgtccttt tttatctatt gcatacttta aacaagataa
     2401 caagcaatta tttattattg aaaatagcac aaaaaccaat cgtgtggata tagccaaacg
     2461 ctttgatgct cagcagcaga actcagccca ctttttgact gttttagata gttaccccca
     2521 aataacgctt tcgactaaaa gtgaccatta tcgattaaga caattgttag aaatgagata
     2581 aagtatgatg aacattttta ttcttgagga cgattttatc cagcagacac gcatagaatc
     2641 tatcgtagta ggtatcttaa aagagactag aataccatgt aatcagcttg aagtgttctc
     2701 gactcctcaa aaattatttg agagtatcca agaaagagga gaccatcagc tgtatttttt
     2761 agatattgaa attggtgaat atactcgttg tgggcttgaa ttagctgcag ctattcgaca
     2821 aaaagatcct aatgccgtta ttgtgtttgt caccactcat tcagaatttg ctcctatttc
     2881 ttttaaatat aaggtatctg ctctagattt tattgataaa gcaggtggtc aaaaacagtt
     2941 taaagaacaa atcgaagaat gtatccgata tacttacgat atgatgtcta gtcgtgagtc
     3001 aaaggacatg tttttatttg agactccaca gacaagatta aaattgcctt ataaggatat
     3061 tttatacttt gctacagcca caactcctca caaagtttgt ttatggactc agacagaaag
     3121 attagagttt tatggtaatc tatctgagat tcaagcagta gcaccgaaac tttttttgtg
     3181 ccatagatcc taccttgtga atctagataa ggttgttcga attgataaga gtaagcagct
     3241 cctttatttc gaaaatggtg attcttgtat ggtatcacga ttaaaaatga aatgtctgtt
     3301 tgaacggtgg gaggctcttc gctaaagtta agagagcaat aacattttag gacgaaaaag
     3361 caacatttca ggaaatgatg ctcttattga agtatttttc gctataataa ttaatgtaaa
     3421 taaagattta cgaagtcatg agtttatcga gcaatcaatc cccaattcaa tcaaaatatt
     3481 gttttctctc tctccataaa caaatagtat ttttgatatg atggctcggc agacagaatg
     3541 acgatgtcag ttgtctttgt ttggatatca cattagctga ttgtaaaatc agtattaaag
     3601 cccggcttgc cgggcttttt gataggtaaa tacagggata agtagctgac gtcttgtgga
     3661 ttctgacttt ttatggttgt tttctgaagg ttattccttt gtcgaaagaa gaactatact
     3721 acgaaaagat ggagtgacaa gaggcagttg aagcacaaaa actagaaata gtacagaaag
     3781 aggcttaatc tgaaagggtt agggcttttt aattttttag gggatgtggt ataatggtaa
     3841 taatttagat tatggagtta aattttgaag aagacctatc gtgtcaagtg tgagaaagat
     3901 ttccaagcca tatttaagga tggaaaaagt acagcaaatc gaaaatttgt catttatcat
     3961 ttaaatagag gccaagacca tttccgtgtg ggtatttctg tcggtaaaaa aataggaaat
     4021 gcagtcacca gaaatgcagt caaacgaaag atacgtcatg ttatcatggc attagggtat
     4081 cagctgaagt cagaggattt cgtggtgatt gcccgtaagg gtgtcgagtc tttggagtat
     4141 caagagcttc aacaaaattt acatcatgtt ttaaagttag cacaattgct tgagaaaggt
     4201 tttgagagtg aagaaaaaca ttaaaattgc aagaatagtc cccttagttc tattattagt
     4261 ggcctgtggt cgtggtgagg taacggcaca atcatctagt ggttgggacc agttggttta
     4321 cttatttgcc agagcaattc aatggctttc ctttgatggt tcaattggtg ttggcattat
     4381 tctttttacc cttactatcc gtctcatgct catgcctttg tttaatatgc aaatcaaatc
     4441 aagccagaag atgcaagaca tccaacctga gcttagagaa ttgcaaaaga aatacgctgg
     4501 taaagacaca caaacgcgga tgaagttggc tgaagaaagt caagcccttt acaaaaaata
     4561 tggggttaac ccttatgcca gtctcttacc cctcttaatt cagatgccag ttatgattgc
     4621 cttattccaa gccttgacac gggtatcttt cttaaaaaca gggactttct tgtgggtgga
     4681 attggcacaa catgatcatt tgtacctttt accagttttg gcagctgtct tcactttctt
     4741 gtccacttgg ttgaccaatc tagcggctaa agaaaaaaat gtcatgatga ctgttatgat
     4801 ttatgtgatg ccgctaatga tctttttcat gggctttaac ttggctagtg gagtagtgct
     4861 ctattggacg gtctcaaatg cctttcaagt ggtacaacta ctgttattaa acaatccttt
     4921 taagattatt gccgaaagac agcgtcttgc caatgaagaa aaagaacgcc gtcttcgtga
     4981 acgtcgtgct cgtaaaaaag caatgaagag aaaataggag tttaggttat ggtattattt
     5041 acaggaaaaa cagtcgaaga agctatcgaa acaggacttc aagagttagg gttatcacgc
     5101 cttaaagcgc atatcaaagt catttcaaaa gagaaaaaag gtttcctcgg gttcggtaaa
     5161 aagccagccc aagttgatat cgaaggtatc agcgacaaga cggtttataa ggctgataaa
     5221 aaagccactc gaggtgttcc agaggatatt aatcaaaaaa atgctcccgc ggtcaattct
     5281 gctgatgtag aacctgaaga aataaaagcc actcaaaagt tagaagcaga agataccaaa
     5341 gtggttcctc tcatgtctga agatagtcca gcgcaaaccc cttctaatct tgctgagaca
     5401 gtcactgaaa gaaaagtaca acaaccgtca atcccagtcg aggaatcaga agtgccccaa
     5461 gatcctggta atgatggttt cagcaaagat attgaaaaag ctgctcagga agtgtccgat
     5521 tatgtgacta aaatcatcta tgaaatggat atcgaagcca ccgttgaaac cagcaacaat
     5581 cgtcgccaaa tcaatttgca gattgaaacg ccagaggcgg gacgtgtgat tggttaccat
     5641 ggtaaggttt tgaaatcctt gcagttgttg gcgcaaaact tcttgcacga ccgctattca
     5701 aaaaattttt cagtatcctt gaatgtccat gattatgtgg aacatcgaac agaaaccttg
     5761 attgacttta cgcaaaaagt tgccaaacgc gtcttggaat ctggccaaga ttacaccatg
     5821 gatcccatga gtaacagtga gcgtaaaatc gttcataaaa ccgtttcatc cattgaaggg
     5881 gttgatagtt attctgaagg caatgaccct aaccgctatg tggtagtcag tcttcaacgt
     5941 tagatcctaa aagaatcttt ggtagttagt ttagctatcg aagatttttt gtatggccag
     6001 cttaaccgtt tgttttactg atctcattga ttattgttgc tattgataag gtttcaacgg
     6061 taaggcaaag gaacagtgtc aaagaagaga cactttatga aatgagcggc ctcttaaatg
     6121 agagagaaac tgttgaaaaa cgaaaataaa tcttgacagt agaccctctt taagatagaa
     6181 tattatagta tgtttagtaa ctgatatcac aaatagccgg ctaaacgaat acaaaatcta
     6241 tgaggaggta ttcactgtga aacgtactta tcaaccaagt aaaatccgtc gtcaacgtaa
     6301 acacggattc cgtcaccgta tgtcaactaa aaacggacgt cgcgtgcttg ctgcacgtcg
     6361 tcgtaaagga cgtaaagtat tatcagctta attgcaaaaa atacaactaa agtaaaccaa
     6421 gagtgctcga ggctcttggt tttttaacgt tttctaatcg gaaatttgca atgtataagt
     6481 atggtgtcaa ctccttacct ttttatttgt tgtatgaaag agcattccaa ataatagaaa
     6541 ataatataaa aaatgaaagt agattctaaa agtctcattg ctattgaaag cgctttattt
     6601 tttgttataa taactttgtg aacgttattt ttattaaaac taagaaacac aaggagtaga
     6661 aactatgcct gataaaccaa ccaaagaaaa gctcatggag cagttaaagg gtgggattat
     6721 cgtttcttgt caggctttgc caggtgagcc tctctattca gagactgggg gtattatgcc
     6781 actgctggca aaagctgctc aagaagctgg tgctgtcggc atcagagcta attcggttag
     6841 agacattaaa gaaattcaag ccataacaga cctaccaatt atcggtatca tcaaaaaaga
     6901 ttacccgccg caagagcctt ttattacggc aacgatggcc gaagtggatc aattagctgc
     6961 cttaaatatt gcagtgattg ctatggattg taccaagcgt gaccgacatg atggcttgga
     7021 tattgctagc tttattcgac aagttaagga aaagtacccc aatcagcttt tgatggcaga
     7081 tattagtacc tttgatgaag gactagtggc ccatcaggct ggcattgatt ttgttggaac
     7141 caccttgtca ggctacaccc cgtatagccg tcaggaagca ggtcctgatg tggcattgat
     7201 agaagctctt tgcaaagcag gcattgctgt cattgccgaa ggaaagattc attccccaga
     7261 agaagccaag aaaattaatg acttaggtgt ggcaggtatt gttgttggag gggccattac
     7321 aaggcctaag gaaattgcag agcgttttat cgaagcgctc aaatcctaat agtggttgtc
     7381 aaactagaaa cacacatcaa aaaaaggaga ttttacgatg aacatgaaga agttagcttc
     7441 attagtgatg cttggtgcat ctgttttagg actagcagct tgtggcggaa agagccagaa
     7501 agaggctggt gcaagtaaat ctgatactgc taagacagaa atcacatggt gggctttccc
     7561 ggtcttcacg caagaaaaag ctgaagatgg tgtgggaacc tatgagaaga aattgattgc
     7621 tgcctttgag aaagctaatc cagaaatcaa ggtaaaattg gaaaccattg attttacatc
     7681 tggtcctgag aaaatcacaa ctgctattga agctggaaca gctcctgatg tcctctttga
     7741 tgctccaggt cgtatcattc aatatggtaa aaatgggaaa ttggctgact tgaatgactt
     7801 gttcacaaaa gagtttacca aagatgttaa caatgacaaa ttgattcaag cctcaaaagc
     7861 aggagataca gcttacatgt atcctatcag ctctgctcca ttctacatgg cgttgaataa
     7921 aaaaatgctc aaagatgctg gcgtccttga ccttgtgaaa gaggggtgga caacagatga
     7981 ttttgaaaaa gtcttgaaag cattgaagga caaaggatat aacccaggtt ctttctttgc
     8041 taatggtcaa ggtggtgacc aaggaccacg tgccttcttt gctaaccttt atagtagtca
     8101 tattaccgat gacaaagtaa ccaaatacac cacagatgat gctaactcta ttaaagccat
     8161 gactaaaatt tccaattgga ttaaagatgg tttaatgatg aatggctctc aatacgacgg
     8221 ttcagctgat attcaaaact ttgcgaatgg ccaaacttcc tttaccattc tttgggcgcc
     8281 tgcacaaccc ggcatccaag ctaaattgtt agaagctagt aaagtggatt accttgaaat
     8341 cccattccca tcagatgatg gcaaaccaga actagaatac ctcgtaaatg gttttgcggt
     8401 ctttaataac aaagatgaac aaaaagtcgc tgcctctaag acatttatcc aatttatcgc
     8461 tgatgataaa gaatggggac ctaaaaatgt tgttcgtaca ggtgctttcc ctgtaagaac
     8521 ttcttatggg gatctttaca aagacaaacg aatggaaaaa attgctgaat ggacaaaatt
     8581 ctactcacca tactacaaca cgattgatgg gtttgctgaa atgagaactc tttggttccc
     8641 aatggttcaa gccatctcta atggtgatga aaagccagaa gatgctttga aagccttcac
     8701 tgaaaaagca aacaagacaa tcaaaaaaac acaataagtg ttcagaacaa gtggcttctt
     8761 ttcacggcac atgtcaaatg gtcactggaa aggagccttt ccttttttag gagagattaa
     8821 tagaaaaaga ggtatttcaa gtgaacgtca ataaattaaa aatgagagag acgctcatct
     8881 catacgcttt cttagctccg gtcttggttt tctttgtgat ttttgtcttg ataccgatga
     8941 ttatgggctt tgtgacgagt tttttcaatt attccatgac ggattttacc tttgttggtt
     9001 ttgctaatta tgctaggatg tttcaagatc ccattttcat gaagtctctt atgaacacct
     9061 tgattattgt tattggttcg gtacctgttg tagttttctt ttccctcttt gtggcggcta
     9121 aaacctacga caaaaatgta gtggcgcgtt cgttttaccg ggcagttttc tttctgcctg
     9181 ttgttaccgg aagtgttgcg gtgacggtgg tttggaaatg gatctatgat ccgatgtcag
     9241 ggattttgaa ttatgtctta aaatatgctc atgtgattga acaaaacatt agttggctgg
     9301 gagataagca ctgggctttg ttagctatta ttgctatttt attaaccaca tctgttgggc
     9361 agcctattat tctatatatt gctgctatgg gaaatattga taattctttg atggaagctg
     9421 cgcgtgtaga cggcgccaca gaatttcagg tgttttggaa tatcaagtgg ccaagtctcc
     9481 tgccaacaac cttatatatt gctgtcataa caaccattaa ttccttccaa tgttttgctt
     9541 tgattcaact cttgacgtct ggagggccaa attattcaac tagtaccctc atgtactatt
     9601 tgtatgaaaa agcctttaag ctctctgaat acggctatgc caacacaatg ggtgtgttct
     9661 tggcagtcat gattgcaatc atcagctttg ctcaatttaa aattttaggt aatgatgtgg
     9721 aatattagga ggatattaag atgacgaaaa agaaactaac cgcatcggat gttctgacta
     9781 ctgttattct atgcatcttg accattttgt ttattttccc attttattgg attatgacag
     9841 gagcatttaa ggctcaagcc gataccatta tgattccacc gcaatggtgg ccaaaggcac
     9901 ctactattga aaattttaag gccttggtag tgcaaaatcc agccttaaaa tggttgtgga
     9961 atagtgtctt tatttcggtg gcgaccatgt tcttggtttg tggcacttcc tctttggctg
    10021 gctatgcctt ggccaaaaag cggttttatg gacaatggat tttgttttcg atttttattg
    10081 ctgctatggc cttgccaaaa caggttgttc tagtgcctct tgtgcggatt gttaatttta
    10141 ttggaatcca tgatactttg gcggctgtta ttttgcctct tgtgggctgg ccatttggtg
    10201 ttttccttat gaaacagttc tcagaaaata ttccaaccga gttattggaa tccgctaaga
    10261 ttgatggatg tggcgaaatt cgtacctttt tcaatgtggc tttccctatt gtcaaaccag
    10321 gctttgcagc gctagcgatt tttaccttta tcaatacttg gaacgattat tttatgcagt
    10381 tggtgatgtt aacctctcgg gaaaatttaa cgatttcact tggggttgcc actatgcaag
    10441 ctgaaatggc tactaattat ggcttgatta tggcaggggc tgccatggct gcagtgccta
    10501 ttgtaacggt ctttcttgtt ttccaaaaat catttaccca aggcattact atgggtgctg
    10561 tgaaaggtta gactaaataa agcgtgtcac taagcttaat ctataggttg cctcttaatt
    10621 taggtgcata gatagataag tcaaaaggga agcaagtcat gtgttaaacg tttaagaggg
    10681 gaattagctt ttgtttgtaa gcgtatttct agaaaggaag ttgtgatttg acatcgaaaa
    10741 aacagggact tttacactct ctcttcaagc ttgatagtaa atggatgagg gctagcgcag
    10801 cactgtttga tttgctcgtg tttaatctct tatttgtctt gtcctgcctt ccccttctaa
    10861 cgattggggt tgctaaaatg gctttgtatg cgagtttgtt ggattggcga gaaggtcaag
    10921 ttagtcagct ttttactact tatagctctt attttaaaca ttattttaaa agagggatgc
    10981 gcctaggtct tattgagctt ttgataatga gtatttgtgt cttagatctt ttcttaattc
    11041 gaaaccaatc aggcctagtt tttcaagggt ttaaagtact ttgcattgct gttttatttc
    11101 tggtagttat actctttttg tacgcttatc ctcaggccgt cagaaaagag ctttccctat
    11161 ctacgctgtt taagcgaagc tttttactag ccggactctt ttttccatgg agttttgctt
    11221 tcttggctct tatttgtctc acgatattta gcctacaatt gtctctgtta acgttatttg
    11281 gaggcgtgtc tttgctagct atcatcggca tcagtagcct aacttacctc tacttgatta
    11341 tcatggaatc ccttcttagg cgattcccac ttaataatga cattgaataa aggacattac
    11401 tatgacagat cttacgaaat accaaggcat tattcctgcc ttttatgctt gttacgatga
    11461 ccaaggagct attagccctg agcgtgtgcg tgccttgacc cagtactata ttgacaaagg
    11521 ggttcagggg ctttacatta atggctcttc tggagagtgt atttaccaga gcgtttttga
    11581 ccgtcaattg gtgttagaaa atgtcatggc agtcgctaag ggcaagctga cgattattaa
    11641 tcacgttgcc tgcaacaaca ctaaagatag tattgaattg gcagctcatt cagaacgttt
    11701 gggcgtggat gccattgcag ctattccgcc tatttatttc cggttaccag aatatgcagt
    11761 tgctgattat tggaatgcga tttcttcggc tgctcctcac acagatttta tcatctataa
    11821 tattcctcaa ctggcagggg ttgccttgac cccaagcctt tataagacta tgttggctaa
    11881 taagcgcgtg attggcgtta aaaattcatc catgccagtg caggatattc aaaccttctg
    11941 tgcgattggc ggagacgatc atattgtttt taatggccca gatgaacaat ttctaggagg
    12001 gcgcttgatg ggtgctgctg caggcattgg tggcacctac ggggctatgc cagaattatt
    12061 tttaaggctg aatcaactta tagttgacaa ggatttagaa aaggccaaga cccttcaata
    12121 tactattaat gagattatcg gtgtccttgt ttctgctcac ggcaatatgt atggggttat
    12181 taaggaagta cttcgtatta atgagggtct tgatattggt tctgttcgat cacctttagc
    12241 tgagttggtc gaggaagata gggttatttg ccaacgggct gctgctctga ttaatcaggc
    12301 taaggaaacc ttttaataag atgcgagggg ataacaaatg aagcattatt tagcaattga
    12361 tattggtggt actgctatca agtatggctt gatttctgaa acaggagacc tgttggaaaa
    12421 agaggaaatg gccactgagg cttataaagg tggtccctct attttggaaa aggtcaaggg
    12481 cttggtaaag acctatcaag accaaatgga tctggctgga gtggccattt cttctgcagg
    12541 aatggttaat cctgataagg gagaaatctt ttatgccggc cctcagattc ctaactatgc
    12601 tggaactcag tttaaaaaag agattgaaga gactttcggg ttaccgtgtg aagtggaaaa
    12661 cgatgttaac tgtgctggtc tagccgaagc tatttctggc agtgccaagg attatcctgt
    12721 ggctctttgc ttgacaattg ggacgggaat cggtggctgt ttattgttta attcccaagt
    12781 ttttcatggt agtagtcatt cagcttgcga agttggttac cttcatttgt cagatggtca
    12841 gtttcaggat ctggcatcta ccacagctct tgttcaagag gtggccttag cttatgggga
    12901 tgacattagc caatgggatg gtcgtcgtat ttttgagcag gctaaagcgg gtgatgcgat
    12961 ttgtatagca gccattagta agcaggtgga ttacttagga caaggtattg ctaatatctg
    13021 ctacgttgtt aacccaaatg tggtggtact tggtggcggt atcatggcgc aaaaagatta
    13081 tttagctgat aaattaaaga cagctcttga tagctacttg gtttctagtc ttgccaagaa
    13141 aactcagctg aagtttgcta gtcatggaaa taatgctggt atattaggag cttactacca
    13201 ttttaaacaa aagaatgagc gatcctgttc atttataact cctgagaaaa tcgaaaataa
    13261 tttagcgagt agctaatctt agtggctgtg agtctatatg acgtgaagag ttcttgttta
    13321 agtatactgg gggaggtgaa tgacgaccct ttaaaaagaa aacaacgccc tttttgctat
    13381 tgtagtctaa aggtcgttat tttctttgtc aataatagtg tttttgaaga ttttttcttt
    13441 gtgcggtttg tcaatcgcta aaacgtaagc ataaataatg tccatcataa taagaagagg
    13501 aaattgtggt gacacacgat tcccataatg tagttggtgg gttgatgata cgggaataat
    13561 atctaaggag tcgtcaaatt cggtttgatt atctgttgtt aagaggacag ttttggcccc
    13621 tcgctgacta gcttgatgaa gtgctgtgat gacagagttt gttttgccag ataaagaaaa
    13681 accaaaaaca aggcaatttt cattgacgag gctatttgcc caagtaaaac catcagtgtc
    13741 tgaataagca tcacagatta aaccaagtcg cataaagcgt agcttcattt ctcttgctac
    13801 tagtccagaa gatcctttgc caaagaaata gactcgctca gaagcatcga tcagcttggc
    13861 taagttcagt aatttttcct cattaacgag ctcataggtt ttattaataa gagcatcgta
    13921 gtccatcagc acgcgctttg tcaattctaa gtgaatagat tggaaagtct cttgtgattc
    13981 ttgaagactg tgaaggtaat caaaggcaaa agcacgataa ccagtaaaac cacacttttt
    14041 tgcaaatcga gtcaaggcgg cttgagagat gtgtagtcgc ttaactatct cggatgcggt
    14101 caactcttga ggtgtcagat cggttgtaat aaaaaaatga gcaatccctt tttctaaact
    14161 ggtcatatgt tccaatgatg cttcgatttt agtaattagg cggtggtttt taggcatagc
    14221 aaagcacact ccttacagtt taactatcta ctagtatagc atagatcgct aacagaaggt
    14281 agcgaccctt tttgtgatac agatttgttc aacccacttt atttgagtag gttgtagtat
    14341 ttttgagggc ttatgtagga gccgactatt aaaggtcatt tgtcaaaaaa gtagaaaata
    14401 gacaagtgtc agctagcaaa gggttttacg atcaacctaa acggcagaag ttctcaccaa
    14461 gttttctgat gtggttaaga tctttatgtt ataataacat catgacaaat aaagtagaaa
    14521 aactaactat ttttgatacc catacgcacc tgaatgtagc agaatttcaa ggacacgaaa
    14581 ctgaagaatt gactttggct caagaaatgg gcgtggccta ccataatgtg gttggctttg
    14641 accaggccac tattagtgga gcacttgctc tcgcagacca gtatcctaac gtgtatgcta
    14701 ctattggatg gcacccgaca gaagcaggct cttattctga agcagttgag gagtctatcg
    14761 tctctcaatt atcacatccc aaagtcattg ccttaggtga aattggctta gactattatt
    14821 ggatggagga ccccaaagag gtgcaaatag aggtttttaa gcgccaaatg caattagcaa
    14881 aagaccatga tttacctttt gtggtgcaca cccgcgatgc cttggaagat acctatgagg
    14941 tcatcaaagc agctggtgtt ggccctcgtg gaggcatcat gcattcttac tcaggatcat
    15001 tagagatggc tgaacgtttc attgaacttg gcatgatgat ttctttttca ggtgtggtca
    15061 ctttcaaaaa ggcccttgat attcaagagg cagcgcagca cttgcctttg gataagattt
    15121 tggtggaaac agatgcccct tatcttacac cagttccaaa acgtggcaaa caaaatcata
    15181 cagcttatac ccgttatgtg gttgataaaa tcgcagagct acggggaatg accgtggaag
    15241 aagttgcaaa agaaacgact gctaatgcaa agagggtatt taagcttgac tgaaaaaatt
    15301 aatattcaag aggttcttgt tgtagaaggc aaggacgaca cggctaacct tcgccgtttt
    15361 tacgaggtgg atacctatga gaccagaggc tctgctatca ctgaagaaga tttagaacgg
    15421 attaaccgcc taaatgactt gcgtggtgtc attgttttga cagacccaga ttataatggt
    15481 gagcgcattc gtaagctcat tatggcagct gtgccaacag ctcgtcacgc ctttttaaac
    15541 cgaaacgagg cggttccgag ttctaagagt aagggccgtt ccttaggggt tgaacatgcc
    15601 aattttgagg acctccaaaa ggcacttgct catgtcactc agcaatatga tgatgagtct
    15661 tattttgaca ttcgtcaaac agacctgatt cgtctagggc ttttaatggc atcagatagc
    15721 cgtaaacgca gggaatactt gggcgaaaag ctccgcatcg gctatgccaa cggcaaacaa
    15781 ctgctcaagc gcctcgaatt atttggcatt accctagcag aagtggaaga agtgatggag
    15841 acatatgagg agtaaagagt acaataaaat aaaacagccc cattaactga atggcgtatc
    15901 ttggcaaaaa agtttaggct agtggttcaa atgccaagaa gaaagtgatc ataaaaagaa
    15961 gagaatatat gagaattgca gattatagcg tgaccaaggc tgtccttgat cgtcacggat
    16021 ttacctttaa gaaatctttt ggtcagaact ttttgaccga taccaatatt ttacagaaaa
    16081 tcgttgacac ggctgagatt gatcagaatg tcaatgttat cgagattggt cctggaattg
    16141 gggctttgac ggaatttttg gcagaaaatg ctgctgaagt gatggctttt gagattgatg
    16201 accgcttggt gccgattttg gcagatacct tgcgcgattt tgataatgtg caagtggtca
    16261 atcaagatat tttaaaagct gatttgcaga cccaaatcaa gcagtttaaa aatcctgatt
    16321 tgcccattaa ggtggtggct aatctacctt actacatcac aacgcctatt ctcatgcact
    16381 tgattgagag caaaattcca ttccaagagt ttgtggtcat gatgcagcgt gaagtggcag
    16441 atcgtatctc ggctgagcca aacactaaag cttatggttc cttgtccatt gcggtgcagt
    16501 attacatgac tgctaaggtt gctttcattg tgccgcgcac tgtctttgta cctgcaccaa
    16561 atgtcgatag tgctatcctt aaaatggtgc gtcgcgacca gcctttgatt gaggttaagg
    16621 acgaagattt ctttttcaga gtgtctcgcc ttagttttgt ccatcgtcgc aagaccctgt
    16681 ggaataactt gactagccat tttgggaagt ctgaagacat caaggctaaa cttgaaaaag
    16741 gtctggcact agcagatatc aagccttcta ttcgtggtga ggccttgtcg attcaagatt
    16801 ttggcaaatt agctgatgct ttaaaagaag ttggattata aaagctttcc cccggtgaca
    16861 ataaaaaaat tcccttgtag acaaaacaga aatgttcagt tcagggggat ttttcttatt
    16921 gaagctggcc gttaaggtat cgcagtgcag agctagtttt ttaatacccc ggaaaaaagg
    16981 ggcaattaac aagagcgtga ctatagcggt acggttagac tctttttcgt aaatagcaaa
    17041 ggtcttctta aaattcacat cgtttcctac ctcaaaaaat catcagtgat ctgattttta
    17101 gttgataaaa tattctattt ttaaaaagca gtgacctaaa tgatagccag tggtctagag
    17161 aatcttgaca gttttttaag gcgagtgaaa cttagagtca aatgctgatt tgggagcggt
    17221 tttctgatat aatagagtta ttatagtagg gcaaaggagc acagttgcaa ggtaaaatca
    17281 ttaaatctct ggctggtttt tattacgtgg agtcagaagg acaggtatat cagacgcgag
    17341 cgcgtggtaa ttttaggaag cgcggtgaga caccttatgt tggagatatc gtggatttct
    17401 cagcagagga caactctgaa ggttatattt tagcgattca tcctcgtaaa aacagtttgg
    17461 taagacctcc tatcgtcaat attgaccaag cagtggttat tatgtctgct aaagaaccag
    17521 aatttaacag caatcttcta gaccgctttc tcatcttgct tgaacacaaa gccattcatc
    17581 cagtggttta tatctctaag atggacttat tagatagtcc cgaggagatt aaggctattg
    17641 gtcgacagta tcaggcaatc ggttacgatt ttgtgaccag tcttgaggaa ttgttgcctt
    17701 tgctagctga taaaattacc gtctttatgg gacaaacagg tgttgggaaa tcaacccttc
    17761 tcaaccgaat cgcccccgaa ttggctcttg aaacaggaga aatctcagat agtctagggc
    17821 gtggtcgcca caccacccga gctgtgagtt tttacaatac ccatggcgga aaaatcgcgg
    17881 atacgccagg tttctcatca ttagactatg atattgctaa tgctgaagat ttgaacgagg
    17941 cttttccaga gctgcgccgg ctgagccatg agtgtaagtt tagatcctgt acccataccc
    18001 acgaacccaa gtgtgctgtc aaggcagcgc ttgaaacggg agagttgtgg ccagttcgtt
    18061 atgagcatta cctgcaattt ctcagtgaaa ttgaaaaccg tcgtgaaaca tataaaaaag
    18121 ttatcaaaag aaagtaggtc tactccatgt caactctaaa aattgcccca tctattctag
    18181 cagctgatta cgctaatttt gcttctgagc tagctcgtat tgaagaaaca gatgccgaat
    18241 acgttcacat tgacattatg gatggtcaat ttgttcctaa catcagtttt ggtgcagatg
    18301 ttgtggcaag tatgagaaag cacagtaaat tggtctttga ttgccatcta atggtggttg
    18361 atcctgaacg ctatgttgag gcttttgcac aagctggtgc tgatatcatg accattcaca
    18421 cagaaagtac tcgtcatatt catggaaccc ttcaaaaaat caaggcagct ggtatgaaag
    18481 caggggtagt gattaatcca ggaactcctg caacagccct agaacctttg ctagacttag
    18541 ttgaccaagt cctcattatg acagtcaacc caggttttgg gggtcaggcc tttatcccag
    18601 aatgtttaga aaaagtggct accgtagcca aatggcgaga tgaaaaagga ttgtcctttg
    18661 acattgaggt tgatggcgga gtagacaata agaccattcg tgcttgttat gaagcagggg
    18721 ccaatgtctt tgtggcaggt tcttaccttt tcaaggccag tgacctagtg agccaagtgc
    18781 agactctaag gacggcccta catgactaaa gtagccctct tttctggggg agatctttct
    18841 tatttcaccc gtgagtttga ttattttgtc ggtattgata gtgggagcct gtttttattg
    18901 gaaaatgggc ttcctttgaa tatggccgtt ggtgattttg attcggtttc ccaagaagca
    18961 tttacggata taaaagagaa ggcagagctt tttattactg ctcatccaga aaaaaatgat
    19021 acggatactg aattagctct taaagaggtc tttgcacgtt tccctgaggc ggaagtgacc
    19081 atttttgggg cttttggggg tcgaatggac cacctcttat ccaatatttt cttgcctagc
    19141 gaccctggta ttgctccctt tatggcacaa atcgccttgc gtgaccaaca aaatatgatt
    19201 acctaccgcc cggcaggtca gcaccttatc catcaagaag aagggatgac ctatgtggca
    19261 tttatggcag aaggagaagc tgacttgacc attactggtg ctaaatttga gttgacgcaa
    19321 gataattttt tcaaaaagaa aatctattcc agtaatgcct ttatccatca acctattaca
    19381 gttagtttgc caagtggcta cttgattatt attcaaagta aagattggag ttagtatgga
    19441 ccttatcttg ttccttttgg tcttggttct cttaggttta ggggcttatc tgttgttcaa
    19501 agtcaacggc cttcaacatc agcttgccca aaccctagaa ggcaacgcgg ataatttgtc
    19561 tgaccaaatg acctaccagt tggatacagc taacaagcaa caattgttag agctaacaca
    19621 gctgatgaac cgacaacaag cagaccttta ccaacaatta acagatattc gtgacgtctt
    19681 gcaccgtagt ttgtctgata gtagggaccg gtctgacaaa cgcttagaaa aaattaacca
    19741 gcaggtcaac caatcgctca aaaatatgca agaatctaac gaaaaacgtt tggaggaaat
    19801 gcgccagatc gttgaagaaa aattggaaga aaccttaaaa aatcgtctgc acgcctcttt
    19861 cgattctgta tccaagcaac tagaaagtgt caataaaggc ttgggagaaa tgcgtagcgt
    19921 ggctcaagat gtgggtactt taaataaggt tttgtccaat accaaaacac gaggcatttt
    19981 aggcgaactt caactaggcc aaatcattga ggatatcatg acatcaagcc agtacgaaag
    20041 agaatttgta acggttagtg gttctagtga acgcgtagaa tatgcgatta agctcccagg
    20101 aaatggtcaa ggcggctata tttacctacc gattgactca aaattccctc ttgaagatta
    20161 ttaccgatta gaagatgctt acgaagttgg tgataaactg gccatcgagg ctagccgaaa
    20221 agcacttcta gcagctatca aacgctttgc caaagacatt cataaaaagt acttgaaccc
    20281 cccagagacg accaatttcg gagttatgtt cttaccaaca gaaggtcttt attcagaagt
    20341 ggtcagaaat gcggctttct ttgatagcct tcgtcgggaa gaaaatattg tggttgcagg
    20401 tccttcgacc ctgtctgctt tgctcaattc cttatctgtt ggtttcaaga cccttaatat
    20461 ccaaaaaaat gctgatgaca tcagtaaaat tttaggcaat gtcaagttag aattcgataa
    20521 atttggcggc ctgcttgcca aggctcaaaa acaaatgaat acagctaata atacgctgga
    20581 tcagctcatt tcaacaagga caaatgccat tgttcgagcc ttgaataccg ttgaaactta
    20641 tcaagaccaa gcaacaaaat ctctcttgaa catgccctta ttagaagagg aaaataatga
    20701 aaattaatca aatgaaaaaa gaccagcttt ttgaagggtt ctacctcatt aagtcggcag
    20761 aggttcgtaa gacaagagct ggtaaagatt ttattagcct tacctttcag gatgacactg
    20821 gcgagatttc aggaaatctg tgggatgccc aaccttataa tgtggaagaa tttactgctg
    20881 gtaaagtcgt gtttatgaaa gggcgacgcg aggtctataa tggtacccct caggttaatc
    20941 aaatcacctt gcgaaatgtt cgcccagggg agcccaatga tccaaaggac ttcaaagaaa
    21001 aggcacctgt cagtgttgaa gaagtacgtg actatttgga acagatgctc tttaaaattg
    21061 aaaatgcaac gtggcaacga attgtaaggg ctctttatcg taagtatgat aaagaatttt
    21121 atacttatcc tgcagctaaa acaaaccacc atgcttttga gtcaggtctc gcctaccata
    21181 ctgctacgat ggtcagacta gcagatagca ttggagatat ctatcctgac ttaaataaga
    21241 gtctactgtt tgcaggtatt atgttacatg acttagctaa agtcatagaa ttaacaggac
    21301 cagataatac agaatacacc gttcgtggta acttgattgg gcatatttcc ctcattaacg
    21361 aagaaattac aaaagtaatt tcagaactac aaatcgatga taccaaagaa gaagtcattg
    21421 tccttaggca cgtgatttta agccatcacg ggcaattaga atacggtagt ccggtacgtc
    21481 cacgcatcat ggaagctgaa attattcata tgattgacaa tattgatgct aatatgatga
    21541 tgatgacaac agctcttagc cgtgttagcg agggcgagat gaccaatcgt atttttgcca
    21601 tggataatcg gtctttttac aagccaaact attgatcagt gtttctcaag taatagttcc
    21661 tttgcttttg tgatgatttt tgatgtgctt aacacttatg atggtttcat aagtgttttt
    21721 ggctcttgtt atggttatta agtagaatgt cctttcagta ggaatgttga ttagtttatc
    21781 ttagattgaa ttcttgcatg ata
//
LOCUS       DAUGHY010000031        21607 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.031, whole genome shotgun sequence.
ACCESSION   DAUGHY010000031 DAUGHY010000000
VERSION     DAUGHY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21607
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.031"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            81..1913
                     /gene="lepA"
                     /locus_tag="VH752_001149"
     CDS             81..1913
                     /gene="lepA"
                     /locus_tag="VH752_001149"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEQ8289511.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDAKK"
     gene            2082..3065
                     /locus_tag="VH752_001150"
                     /pseudo
     CDS             2082..3065
                     /locus_tag="VH752_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164232585.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            3251..3688
                     /gene="msrB"
                     /locus_tag="VH752_001151"
     CDS             3251..3688
                     /gene="msrB"
                     /locus_tag="VH752_001151"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ8289512.1"
                     /translation="METSDELKQRIGDLSYEVTQHAATESPFTGEYDDFFEKGIYVDI
                     VSGEVLFSSLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAGSHLGHV
                     FSDGPKEAGGLRYCINSAALKFIPYDQMEKEGYAQWLTLFDET"
     gene            3818..4837
                     /locus_tag="VH752_001152"
     CDS             3818..4837
                     /locus_tag="VH752_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEQ8289513.1"
                     /translation="MSTHLRKLPGLLLCLLLALPAWCLGRLFPIIGAPVFAILLGMLL
                     ALFYEHRDKTKEGISFTSKYILQTAVVLLGFGLNLTQVMAVGMQSLPIIISTIATALL
                     VAYGSQKWLRIDVNTATLVGVGSSICGGSAIAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNEGFAIFAGTAVNDTSSVTATATAWDALHHSNTLDGATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEEGFSLRKSFPRFILFFLLASLITTLMTSLGVS
                     ADVFHSLKTLSKFFIVMAMAAIGLNTNLVKLIKTGGQAILLGAICWVAITLVSLAMQL
                     SLGIW"
     gene            5044..5469
                     /locus_tag="VH752_001153"
     CDS             5044..5469
                     /locus_tag="VH752_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ8289514.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDDFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPGEALTKDEIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            5488..5979
                     /locus_tag="VH752_001154"
     CDS             5488..5979
                     /locus_tag="VH752_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ8289515.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRATDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            5996..6805
                     /locus_tag="VH752_001155"
     CDS             5996..6805
                     /locus_tag="VH752_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ8289516.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            6802..7629
                     /locus_tag="VH752_001156"
     CDS             6802..7629
                     /locus_tag="VH752_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ8289517.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            7765..9414
                     /locus_tag="VH752_001157"
     CDS             7765..9414
                     /locus_tag="VH752_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8289518.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDDLGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPNQGLLFASNPHLGNVFSKSVYIQEVLRAAHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPAKTLTSELVIADKLDNTFTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLAVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            9418..10206
                     /locus_tag="VH752_001158"
     CDS             9418..10206
                     /locus_tag="VH752_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8289519.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEATKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLQTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            10200..11246
                     /locus_tag="VH752_001159"
     CDS             10200..11246
                     /locus_tag="VH752_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ8289520.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNVAMCIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDV
                     YESVAEGKMIVGLTYEDPCINLQKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAK
                     QFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKIL
                     NTYNRLHEALERGK"
     gene            11361..12758
                     /locus_tag="VH752_001160"
     CDS             11361..12758
                     /locus_tag="VH752_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEQ8289521.1"
                     /translation="MTYQTIYPYTNEVLHTFDNMTDQGVADVLERAHLLYKKWRKEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVDLCADIADYYADKADEF
                     LMSTPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFAPNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEAGSITNLFISYDQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIIFDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQAKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGIKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNSNTKV"
     gene            12860..14656
                     /gene="uvrC"
                     /locus_tag="VH752_001161"
     CDS             12860..14656
                     /gene="uvrC"
                     /locus_tag="VH752_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEQ8289522.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPFKKCKNPINKVCF
                     YYHLGQCCAHTICHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASEEMAFERA
                     AEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDVNL
                     FPYYNDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGEKK
                     QLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLRIDKPVRIEAFDNSNIQGT
                     SPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPNLI
                     IVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSEEF
                     FLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIASA
                     TSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
     gene            14840..15442
                     /locus_tag="VH752_001162"
     CDS             14840..15442
                     /locus_tag="VH752_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEQ8289523.1"
                     /translation="MKFLELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQP
                     WKFVVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDD
                     LIGYYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTT
                     NEILDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIERR"
     gene            15567..16976
                     /gene="pepV"
                     /locus_tag="VH752_001163"
     CDS             15567..16976
                     /gene="pepV"
                     /locus_tag="VH752_001163"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEQ8289524.1"
                     /translation="MTTIDFKAEVDKRKEAMLADLVDLLRINSERDDTLADDKHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFGHLDVVPAGSGWDTDP
                     YEPVIKDDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNPDFGFSPDAEFPIINGEKGNITEYLHFAGDNNGAFVLHRFQGGLREN
                     MVPESATAVITSPHDLDVLEAALEQFLSEHGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGVNGATLLAKFLNQFTFEGAAKDYLYVAGEVLHEDFAAEKLGLAYTDDRMGALSMNA
                     GVFTFDSQSADNTIALNFRYPKGTDAATLKAGLEKLPGVIKVSLSEHEHTPHYVPMDD
                     ELVATLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
     gene            complement(17044..18420)
                     /gene="mnmE"
                     /locus_tag="VH752_001164"
     CDS             complement(17044..18420)
                     /gene="mnmE"
                     /locus_tag="VH752_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEQ8289525.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQDSNRI
                     ILLNKTDLEQKIELEQLPADLIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     misc_feature    18592..18727
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            18756..19256
                     /locus_tag="VH752_001165"
     CDS             18756..19256
                     /locus_tag="VH752_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEQ8289526.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     gene            19321..19686
                     /gene="rplL"
                     /locus_tag="VH752_001166"
     CDS             19321..19686
                     /gene="rplL"
                     /locus_tag="VH752_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEQ8289527.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKSKLEEAGATITLK"
     gene            20251..20543
                     /locus_tag="VH752_001167"
                     /pseudo
     CDS             20251..20543
                     /locus_tag="VH752_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850651.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20718..21043
                     /locus_tag="VH752_001168"
                     /pseudo
     CDS             20718..21043
                     /locus_tag="VH752_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            21034..21607
                     /locus_tag="VH752_001169"
                     /pseudo
     CDS             21034..21607
                     /locus_tag="VH752_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261819.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
BASE COUNT         6684 a         3915 c         4596 g         6412 t
ORIGIN      
        1 ataatccgta agaaaagata ggcaaaggcc tacctttttt gttataatag aaggaaagac
       61 taaatgtata ggaaaataga atgaacagtc aagatttaaa aaaacgtcag gagaagattc
      121 gtaatttctc cattattgca catattgacc atggcaaatc tactctcgct gaccgcattt
      181 tggaaaagac agagacggtt tcgtctcgtg aaatgcaggc tcagttattg gactccatgg
      241 atttggagcg tgagcgtggg attaccatca agttaaacgc cattgagctt aattacacgg
      301 ctaaagatgg ggaaacctac attttccacc ttattgatac cccagggcat gtggacttta
      361 cttatgaagt atcgcgttcg ttggcagcct gtgagggagc gattctagtt gtggatgcag
      421 cgcagggtat tgaagcgcag acccttgcta atgtttacct agcccttgat aatgatttgg
      481 aaattttacc ggttatcaat aaaattgatt tgcctgctgc tgaccctgag agagtccgcc
      541 atgaagtaga agatgtcatt ggacttgatg cttctgaggc tgttctggcg tcagccaagg
      601 ctggtatcgg gatcgaagag attcttgagc aaattgttga gaaggttcct gctcctacgg
      661 gtgatgtgga tgcgccttta caggccttga tttttgactc tgtttacgat gcttatcgag
      721 gcgttatcct gcaggttcgg attgtgaacg gtattgtcaa gcctggcgat aaaatccaga
      781 tgatgtcgaa tggaaaaacc tttgatgtca cagaagttgg gattttcact cctaaggcag
      841 ttggacgaga tttccttgca acgggagatg ttggttatgt tgcggcttct atcaaaacgg
      901 tagcagatac ccgtgtgggg gatacggtga ctttagctaa taaccctgct aaagaagcct
      961 tgcatggcta caagcagatg aacccgatgg tctttgcagg gatttatccg attgaatcaa
     1021 ataaatacaa tgacttgcgt gaagcgcttg aaaaattaca gcttaacgat gccagtttac
     1081 agtttgaacc cgaaacgtca caagcgcttg gctttggttt tcgatgcggc tttttaggct
     1141 tgcttcacat ggatgtgatt caagaacgtt tggagcgtga gtttaacatt gatttgatca
     1201 tgacagcacc atctgtagtc taccatgttc acacaacaga tgaggacatg attgaagtgt
     1261 ctaacccttc agaatttcca gacccaaccc gggtggcttt tattgaagag ccttatgtta
     1321 aagcgcaaat catggtgcca caagagttcg taggggctgt tatggaatta tcgcagcgca
     1381 agcgtggtga ttttgtgacc atggattaca ttgatgataa ccgtgtcaat gtgatttacc
     1441 aaatcccgct ggctgaaatt gtctttgatt tctttgataa attgaaatcc tcaacgcgag
     1501 gctatgcgag ttttgattat gatatgtcag aataccgcag gtcacaattg gtgaaaatgg
     1561 acattctttt gaacggcgat aaggtcgatg cgcttagctt cattgttcat aaagaatttg
     1621 cttatgaacg cgggaaaatt attgttgaga aattgaaaaa aatcattcca cgtcaacaat
     1681 ttgaagtgcc gatccaagcc gctattggtc aaaaaattgt tgcgcgttca gatatcaagg
     1741 ctcttcgtaa aaacgtttta gcgaagtgtt atggtggtga cgtgtctcgt aaacgtaaat
     1801 tgctcgaaaa acaaaaggct ggtaaaaaac ggatgaaggc tattggttct gtcgaagtcc
     1861 ctcaagaagc cttcttgagt gttctttcaa tggatgacga tgccaagaaa taaccaagcc
     1921 taatcgctta gtcttcagag gagaactctt gtgggcctac atgaccaaaa aattgattat
     1981 ctcataaaaa aagaacttta caatctcttt cacataacac tataatggta ttatagtgtt
     2041 atctcattat taggtgagcc tgcatttaat cttattaaag agaaagagga aacaaaaact
     2101 gtgaacaaaa caaaacaaaa catagccttc tttgtcgcta tggcttaacg tcagcggctg
     2161 ctcttttgct ggcctttgga ggtgcaagtg cggttaaggc ggatcaacag ccaactattg
     2221 aagagacaca gagtcaatta caggacttac gcgaagcttt ccctaaacta gaagatagtt
     2281 atcaagagtg ggatatcttt aggcaaaaca aaatttataa gcatttagat gagtttgagc
     2341 ataagctaaa tagctatttg aaagtattag atggaagttt atctcagtta aagggtgaaa
     2401 aaggtgaacg tggtccagta ggtcccgctg gtccacaagg ccttcaaggt accaaaggtg
     2461 accgcggtga aactggtgaa caaggtcaac gaggcgaaac cggtcccgct ggcccacaag
     2521 gtcccgcagg tccagtaggt ccagctggca aggacggcca agatggtaaa gatggtcttc
     2581 caggcaagga cggccaaaac ggtaaagatg gtcttccagg caaggacggc caaaacggta
     2641 aagatggtct cccaggtaaa gacggtaagg acggccaaga tggcaaagat ggtcttccag
     2701 gtaaagacgg taaagatggc ctcccaggta aagacggtaa ggacggtcaa ccaggtaaac
     2761 cggctcctaa aacaccagag gtccctcaaa aaccagatac cgcaccacat acaccaaaaa
     2821 cccctcagat tcctggccaa tcaaaagacg tgacacctgc ccctcaatcc ccttctaata
     2881 gaggtctaaa caaaccacaa acacaaagtg gtaatcagct cgcaaaaaca ccagcagctc
     2941 acgacacaca cagacaattg ccagcaacag gcgaaacaac caatccattc tttacagcag
     3001 ctgctgtagc tatcatgacg acagctggag ttgtagctgt tgcaaaacgt caagaaaaca
     3061 actaatcctc tttttgctac ctaactgatg gaaagtaaaa ctcagagaga acctcaaggt
     3121 tctctctttt ttgaatcaag atagtagctc taggtataac gtcttgtctg aggtgctgtg
     3181 aacaaatcag ttactgctca cctctttttt tggtaaaatg taagcactaa aacatcaaaa
     3241 ggagttttcc atggaaacat cagatgagtt aaaacaacgg attggtgatc tttcttacga
     3301 ggtcacccag catgcagcaa cagaatctcc ctttacaggg gaatatgatg atttttttga
     3361 gaagggtatt tatgtcgata tcgtcagtgg tgaggtcttg ttttcatcac tggataaatt
     3421 taattctggc tgtggttggc ctgccttttc aaaacctatt gaaaatcgta tggtcacaaa
     3481 ccacgatgac tcttcttatg ggatgagacg agttgaggtt aaaagccgtg aagcaggatc
     3541 gcatttgggg catgtcttta gtgacggccc caaagaagct ggaggcttgc gttactgtat
     3601 caattcagca gctcttaaat tcattcctta tgaccagatg gaaaaagaag gctatgccca
     3661 atggctcaca ttgtttgatg agacataaca gggtctcaaa atgactaaaa aagaagcgtc
     3721 agtgattccc ataagcactg gattggtgag gcgcacgggt caattttaca agcctttgta
     3781 aaaggtctat aatagggaat gaaaaaagga ggttagtatg tcaactcact taagaaaact
     3841 tccaggcctg ttactttgct tattgttagc tcttccagcc tggtgtttag ggcgcttgtt
     3901 tcctattatt ggagcacctg tttttgctat tcttttagga atgttgttag ccttgtttta
     3961 tgaacatcgt gacaagacta aagagggaat tagttttaca tccaagtata ttttacaaac
     4021 agcagtggtt ttgcttggtt ttggattaaa cctaacccaa gttatggcag tgggcatgca
     4081 gtctttaccg attatcattt caactattgc gacagctctt ttggtagctt atggctcaca
     4141 gaaatggctg cgcatagatg tcaatacagc caccttggtg ggtgtagggt cttccatttg
     4201 tggggggtct gctattgcag cgacagctcc tgtcattaag gcaaaggatg acgaggttgc
     4261 taaggcaatt tcagtcattt ttctctttaa tatgttagca gctttgctat ttccaagtct
     4321 aggacaatta ctgggcttat ctaatgaagg ttttgctatt tttgctggga cagctgttaa
     4381 cgacacctct tcagtaaccg cgacagcaac agcctgggat gcccttcacc attccaatac
     4441 actagatggc gcaaccattg tcaaattgac tcgcaccttg gctattcttc caattacttt
     4501 aggcttatcc ctttaccgag cgaaaaaaga gcacgacatc gttacagaag aaggcttcag
     4561 ccttaggaag tcattccctc gcttcatcct cttcttttta ttagcttccc tcatcacgac
     4621 attgatgacc agtttgggtg tttctgccga tgttttccat tccctaaaaa ccttatcaaa
     4681 attctttatc gtgatggcta tggcggctat cggtttaaac acaaacttgg ttaaactgat
     4741 taagacgggc ggtcaggcta tccttttagg ggctatttgc tgggtagcta tcacccttgt
     4801 cagtttagcc atgcaattaa gtttgggcat ttggtaatca gacacgagaa atcagtttga
     4861 ctgatttctt tttttgtatc ttatttgtgt agaattgtct acattttaaa gagaataatg
     4921 cattttaaaa acgttttcat ttggtaaaat aaacctatcg ttaaaccagt tcagtaaaga
     4981 caactggttg agataagcaa ttaccgttta ttcagcggtt tttgttaata gaggaggata
     5041 ggaatgaaac gaaaatttct catcgggagt cacggcagac tagccagtgg tttgcaaagc
     5101 tctattgaca ttttagcagg tatgggacag gcacttgaaa ccattgatgc ttatgttgat
     5161 gatagtgact acactagcca gattgatgac tttatcgctg gggtagcagc agatgagcag
     5221 ggccttattt ttactgattt gttaggcggc agtgtgaatc agaaaatggt aaccgctgta
     5281 atgaacagtg gcaaagacaa tatcttttta atcacaaaca gtaacttagc cactttattg
     5341 tcgttggtgt ttttaaaacc aggcgaagcg cttactaaag atgaaattgt aaccgttatc
     5401 aatgaatcgc aagttcagtt agtggatttg gtccctgaga caaattcaga agatgacttt
     5461 tttgattaat atctaggagg aagacaaatg attactcaaa ttcgtgttga cgaccgtttg
     5521 attcacgggc aagtggcagt tgtatggacc aaagagttaa atgcgccgct tttagtagtg
     5581 gctaatgatg aagctgcaaa aaatgaaatc acacaaatga cgcttaaaat ggctgtgcca
     5641 aacggcatga aactgcttat tcgttcagtt gaagactcga ttaagctctt taatgatcca
     5701 cgcgcaactg acaagcgtat ttttgtgatt gtcaattctg ttaaagatgc ttgtgccatt
     5761 gctaaagaag ttccagactt agaagctgtt aatgtggcta atgtggggcg ttttgacaaa
     5821 tcagacccag caagcaaagt caaagtgaca ccaagtcttt tacttaaccc agaagaaatg
     5881 gcagccgcaa aggaattagt cagtttacca gaacttgatg tctttaacca agtcttacca
     5941 tcaaatacca aggtccattt gagccaatta gtcaactaaa gagaggtaaa aaagtatgtt
     6001 agtaccagca acaatggcag ctttagctgt attgatttgt tttgggggaa attatttaac
     6061 cggtcaaagt atgatggaaa gacccctagt agttggactg gtcacaggtc ttcttttagg
     6121 agatatgaag gtcggtatct taatgggggc ctctctagaa gctcttttcc taggaaatgt
     6181 gaatattggg ggtgttattg cagctgagcc tgtaacagcg actgccatgg caacaacctt
     6241 taccattatt tcacatattg accaaaaagc agccatgacc ctagctgttc caattggaat
     6301 gttggcagcc tttgtggtta tgtttttgaa aaacgtcttc atgaatatct ttgctccaat
     6361 ggttgataaa gctgcagcag ctaaccatca aggtaaacta gtgatgctcc actatggcac
     6421 ttggattatc tattacttaa tcattgcctc tatttctttc attggtattt tagtaggtag
     6481 tggacctgtt aatgcctttg tggaacacat tcctcaaaat ctcatgaatg gcttgagtgc
     6541 tgcgggaggc cttcttccag ctgtcggttt tgccatgctg atgaaattat tgtggaccaa
     6601 caaattagct gttttttacc tattgggttt tgtcttaaca gcttatttga aattaccagc
     6661 ggttgcagtt gctgccctag gagcagttat ttgcgttatt agttcccaac gtgatctaga
     6721 attagacgca attactagag gtgcaattag taaacaaaca acctttgact ctaaagaatc
     6781 agaagaggag gacttcttcg catgacatca caagataatt tgactaaaga agaccgcaaa
     6841 atgcttcgct cagtcttttg gcgctcatgg acgatgaatg ctagtcgtac tggggcaaca
     6901 caataccacg cagtaggggt tatttacact ttactaccag tcatcaatcg tttttataag
     6961 actgataagg ataaagcaga ggcactggtc cgtcatacaa catggttcaa tgccaccatg
     7021 cacatcaata actttatcat gggtcttgtg gcatcgatgg aaaagaaaaa cagtgaagat
     7081 cctgattttg atgctagtgc tatcaccgct gttaaggctt cgttgatggg acctatatct
     7141 ggagttggag attccttctt ctggggaatt ttaagggtta ttgcggcagg tatcgggatt
     7201 tccttggcaa gtgcaggctc agccatggga gctgttgtct tcttactctt gtacaatatt
     7261 cctgctttta ttattcacta ctatagcctt tatggtggtt attctgtagg tgcaggattt
     7321 atcaaaaaac tttacgaatc tggtggtata aaaatcgtca ctaaaacctc aagcatgctt
     7381 gggttaatga tggtaggctc aatgacggct tcaaatgtaa aatttaaaac gattcttaca
     7441 gttgctgcga aaggtgccaa agaagcagct tctattcaag attaccttga ccaattgttt
     7501 attggtattg taccattaat ggtaacacta gcagcttttt ggcttctacg aaagaaagtg
     7561 aatatcaact ggattatgtt tggtatcatg ttccttggca ttatccttgg acttttaggt
     7621 atttgctaat aatctcctag caatgaaggt aagtaagaca agttttcttg ttttgcttac
     7681 ctttttcctt ttaagatgat gagacttgtt ttgatgaaaa acgtagtagc attaagaaat
     7741 ttgttataat gaaaggagct taagatgcga ggtgaacagg tggaagaaca ttttaaaaaa
     7801 caattacaag atgatatttc tagacatttt tcgtaccaat cgttaatgtt atcattgttg
     7861 ctaattggtc tttttattat tttttcatta gcgccacaac aattaggcct ctatcgtgat
     7921 atcaatgcca ctgcaacccg ttatcaccgt ttgattagca aacaagaagc cttgctggat
     7981 gacttaggga aaaatagctt actacctttt ttaaataaaa acctcagcac tgctgattta
     8041 agcaagcact attttcattt gcgccatagc agccaaacgt caccagaact tttactattt
     8101 tcacctaatc aaggcctttt atttgctagt aacccacatt taggaaatgt ttttagtaaa
     8161 tctgtttata ttcaagaagt cttgagagca gctcatcctc caaaaacctt gtttaaagtt
     8221 gctatggata gtgaagatgg tcactacttg atgatcatta agccaatgat agatcaaaac
     8281 caactaaagg gatatgcttt tttagtgatg agtggtaaag attttcttca tcctgcaaaa
     8341 acattgacgt cagagttggt cattgctgat aagctggaca atacgtttac gttttccaat
     8401 cgtgagttta tagcatctag tctagataag atcaacagtc agtatttaca ccattatttt
     8461 gtttttcaag ataatcgagc ctttatcaca agaaaggttg ccttacaagg aggtctttgg
     8521 ctttacatgt ataggccctt gattccaatg gtatcggtga tgttattttc acttatttca
     8581 tcagctgtta tctttgtgat tttgcaacgt aaatcaagtg gtttagctaa ccgaattgca
     8641 gctaaaaatt caagagcaat caatcaaatg gttagagata tgagtgcgat ctctcggcaa
     8701 gaaaaaagac gtattgacct tgagagtcaa gatgaatttc aatatttatc tgatcaaatc
     8761 aatcaaatgg tagagcgatt gcaacagtta catgataaaa cgttagattt ggaaactcaa
     8821 aaattattat ttgaaaaacg gatgttagaa gctcaattca atccgcattt cctctacaat
     8881 acgctagaaa ccattttgat tacgagccat tatgattctg ccctaacaga aaaaattgtt
     8941 atccaattga caaaattgtt gcggtatagt ctcacggatt ctagcaaacc tgtcttgctc
     9001 aaagatgatt taagtgtcat cgagtcttat ttagtgatta atcaggtacg gtttgaagaa
     9061 ttgcaatata gcattaacct atccccagac ctcgatagtc ttgcagtgcc taaattgttc
     9121 ttgttacctt tgatagaaaa tgccatcaaa tatggcttga aagaacgaca cgatgtcaag
     9181 attaacattg cttgttatta tcaagatgac cacattattt tttctgtgag agataatggt
     9241 tcgggaattg atgctcatca ccaaaaagtg attcgagagc aattggaagc tggagagtca
     9301 caccatgggt taattaactc ttatcgtcgg cttaagtatc atttttcaga agtatccttg
     9361 gtttttgatc aaggtgataa acagtttaat gttagctacc atgttaagga gtagtctatg
     9421 tacaaattag tcattattga agatgaacat attattagga aatggttgcg atacgctatt
     9481 gattataaag ctttagatat tttggtcatt ggcgaggcta aagatggcaa agaaggagct
     9541 gttttaatca aagagagtca accagatatc gttttaaccg atattaacat gcccatcatg
     9601 acagcttttg acatgtttga agcaacaaaa gatcaaactt acgccaaaat tattttatct
     9661 ggctatgccg attttccaaa tgcaaggtct gccatacact acggtgtact tgaattttta
     9721 acaaaaccga ttgaaaaagc agctctttgg gaatgtcttc aaaccattat tgctaaaata
     9781 gaaaagcaaa aggggtctaa tcaaaaaaca gatgcttgcg tctatattcc cttaccccag
     9841 atgactgatc agattcctga agtagtcaaa gatattttgg agtgggtaca tgctcatttt
     9901 caagacaaga tcagcacgag ccgcctagct catgacttag ggtatagtga gagttatatc
     9961 tatcaaaata tcaaaaagca tcttcagatg cctttaagtg attacattaa tcaatatcgg
    10021 attaatcagg caattcaatt gatgcaacaa gaaccagatt taatggtata tgaaatagca
    10081 caagctgtag gtatttatga ctatcgctat tttgataggg tttttaaaaa gtatctagga
    10141 caaactgtaa aggcttttaa agaagagcat tttatgaaag ataccgatag gagtaagcca
    10201 tgttaaaact caaacgcaaa tggctattaa gctttttgtt agtaataatt attttagcaa
    10261 tcgttaacgt tgcaatgtgt atattctcat cgtcaaaaaa agatagtgct aaagaattag
    10321 ttatcttgac tcctaatagc caaactattt taacagggac tattccagcc tttgaggaaa
    10381 agtatggggt taaagtaaga ttaatccaag gtgggacggg ccaacttatt gatcaattag
    10441 gtcgaaaaga taagccatta aacgctgata ttttctttgg tggcaattac actcaatttg
    10501 aaagccataa agatttattt gaatcctatg tttctccgca ggtttctact gtcatttcag
    10561 attaccaatt gcctagtcat cgtgcaaccc catatacgat caatggcagt gtactgattg
    10621 ttaataacga attagcaaga ggacttcata ttaccagtta tgaggatttg ctacaaccag
    10681 ctttaaaagg caaaattgct tttgctgacc caaatagctc atctagcgct ttttcacaat
    10741 taacaaacat cttactggct aaaggaggct atacgagctc agaagcttgg gagtatgtga
    10801 aacgtttatt actgtctatg aattctatca gagcaacaag ttcttcagat gtttacgaat
    10861 ctgttgcaga aggtaaaatg attgttggtt tgacctatga agatccttgc atcaatctgc
    10921 aaaaaagcgg tgcaaacgtt tcgattgttt atcctgatga ggggacagta tttgttccgt
    10981 catcagtcgc tattatcaaa aaagcaaagg caatgaaaga agctaagcaa tttattaatt
    11041 ttattttatc atcagatgta caaaatgcct ttgggcagtc aactagtaat agacctatcc
    11101 gacgagatgc tcaaacaagt catgaaatga aaccattaga tgaaattatt actttaaaag
    11161 aagattacgg atatgttact agacataaga aaaaaatatt gaatacctat aatcgactac
    11221 atgaagcatt agaaagagga aagtagactc taggcttgtt gttgctatac tagtagtgtt
    11281 acaaaatagc gtagtcattt tttgaaatcg atttcataat ctggtagact aaggttatca
    11341 cgaaaaggag gctatatgtg atgacttatc aaactattta cccttataca aatgaagtcc
    11401 ttcatacatt tgataatatg accgaccaag gtgtggcaga tgtccttgaa agagctcacc
    11461 ttctctataa aaaatggcgc aaggaagatc atttagaaga acgtaaagcc cagcttcatc
    11521 aagtggctaa tattcttcgc cgtgaccgcg ataaatatgc tgaaatcatg accaaagaca
    11581 tgggaaaact gttcacagaa gcccaaggtg aagtagacct ttgtgccgat attgctgatt
    11641 actacgctga taaggcagat gaatttttga tgtctacgcc actagaaaca gattcaggtc
    11701 aagcttacta tttgaaacaa tcaactggtg ttattctagc cgttgaacct tggaatttcc
    11761 cttactatca aatcatgcgg gtttttgcgc caaactttat tgttgggaac ccaatggttt
    11821 taaaacacgc gtccatttgc ccacgctctg cccaatcttt cgaagaatta gttcttgaag
    11881 ctggtgctga agcaggtagt attaccaatt tgtttatttc ttatgaccaa gtatcacaag
    11941 tgattgctga taaacgtgtg gttggtgtct gcttgacagg ttccgaacgt ggaggagcta
    12001 gcattgcaga agaagctggt aaaaacttga aaaaaacaac ccttgaattg ggtggagatg
    12061 atgcctttat tatctttgat gacgcggatt gggatcaatt agagaaggtg ctttacttct
    12121 ctcgtctcta caatgcagga caagtatgta cctcatcgaa acgttttatc gtgcttgaca
    12181 aagactatga ccgtttcaaa gaattattaa caaaagtctt caaaacagct aaatggggtg
    12241 acccaatgga tcccgaaacg acgttggcgc cattatcatc agctcaagct aaagcagatg
    12301 ttcttgacca aattaagttg gccttggatc atggtgcgga gttggtttat ggtggtgaag
    12361 cgattgatca tccaggtcat tttgtcatgc caaccattat tgctggcctc acaaaagaca
    12421 accctattta ctatcaagaa atctttggcc cagtcggtga aatctacaaa gtgtcttctg
    12481 aagaagaagc gattgaagtg gcgaatgatt ctaactatgg ccttggaggt actatcttta
    12541 gtagcaatca agaacatgcg aaagccgtag ctgctaaaat tgaaactggt atgtctttta
    12601 tcaactcagg ttggacaagt cttccagaac tcccatttgg tgggattaaa cattcaggct
    12661 atggtcgtga attgagtgag cttggcttta catcttttgt gaatgaacac cttatctata
    12721 ttccgaataa aacgaataat agcaatacta aagtttgatc tagattgtta aaggagatct
    12781 gctcgcctta ataaacaatc aaggcgttac actcttcttt tttaaagtca aaattttaaa
    12841 aatttgataa actagtaaca tgaacgaact gattaaacat aaactagaac tcttaccaga
    12901 cagccctggc tgttacttac ataaagataa agaaggaact attatctatg tcggtaaggc
    12961 aaagaatttg aaaaaacgcg tccggtccta ttttcgtggg agccatgata ccaaaacaga
    13021 gttactagta tctgagattg tagatttcga atatattgta acagagtcag ataccgaagc
    13081 gcttttgttg gaaattaact tgattcaaaa aaatatgcct aagtacaaca tcaagttaaa
    13141 agatgataaa tcttatccct ttctcaaaat cacaaatgag tcttttcctc ggttagtgat
    13201 tacaagatat attaagaaaa atgatggatt gtactttggg ccctacccag attcttacac
    13261 tgctaatgaa gttaaaaagt tattagatag gatttttcca tttaaaaagt gtaaaaatcc
    13321 tatcaataag gtctgttttt attaccattt aggccagtgc tgtgctcata ccatttgtca
    13381 tacagacaag gcctattggg atcgtttaat tgatgatgtt aagcactttt taaatggtaa
    13441 agatgacaaa attattgaag atcttcgttc taagatgtta gcagcttctg aagaaatggc
    13501 atttgaacgt gccgctgaat atcgagattt gatttcaggt attgccacca tgcgcaccaa
    13561 gcagcgggtg atgagcaagg atttgcaaga tagagatatc tttggctatt atgttgataa
    13621 gggatggatg tgtgtccaag ttttctttgt gcgtcaaggg aaattaattc agcgtgatgt
    13681 taaccttttt ccctattata acgatgcaga agaagatttc ttaacttata tggggcaatt
    13741 ttaccaagat aaacagcatt tcatccctaa agaagtcttt attccagaag ctattgatga
    13801 agaactggta gccgctattg tgccaaccaa aatcatcaaa cccaaacgcg gtgaaaaaaa
    13861 acaactggta gctttggcta ctaagaatgc tcgtgttagt ctgcaacaaa aatttgattt
    13921 attagaaaaa gatatcaaaa aaacgagtgg tgctatcgaa aatttaggtc agttacttag
    13981 gattgacaag cctgtgcgca ttgaagcctt tgataattct aacattcaag ggacaagtcc
    14041 agttgcggct atggttgttt ttgttgatgg caaacctagt aagaaagatt atcgtaaatt
    14101 taagattaaa acagtggtgg gaccagatga ttatgccagt atgagagaag tgctttttcg
    14161 aaggtacagt cgggtgaaaa aggagggact gcaagctcct aacttgatta ttgttgatgg
    14221 aggcgttgga caagtcaatg tggccaaaga tgtgatagaa aaacaacttg gactcactat
    14281 tcctgttgcg ggacttcaaa aaaacgataa gcaccagacc catgatttgc tttttggaaa
    14341 cccgcttgaa gttgtaccgt tgccgcgccg ttctgaagag tttttcttac tgcatcggat
    14401 tcaagacgaa gtgcatcgct ttgcagttac atttcataga caagtgcgac gtaaaaattc
    14461 tttttcatca acattagatc atatttcagg gcttggccca aagcgaaaac agttattact
    14521 aagacacttt aaaaccataa cagcgattgc atcagctacc tctgaggaga ttcaagcttt
    14581 aggtatacct aaaacagtag ttgaagcaat acagcaacag ataacagaca ataaaaatga
    14641 tagatcgtct ccttaaataa gtgttatgtt ttcagtactg atgaggtaaa aaggcgctac
    14701 ttgttacgca cgacatttca gttcgttatt ttgaaacttt cagaaagttg cgaagtgtag
    14761 tgagttgatg attttaaaaa ctattttatg gtaaaataaa atggtataaa aaaaacagat
    14821 ttagatagga agttttgaaa tgaaatttct agagttaaac aaaaaacgcc atgccattaa
    14881 gacctttaat gaccagccag ttgactacga ggatttgcga acagccattg agattgcaac
    14941 cttagcacct agtgccaata acattcaacc ttggaaattt gtagtcgttc aagagaaaaa
    15001 agctgagtta gcaaaagggt tacctctagc aaataaagtg caggttgaac aggctcaata
    15061 tgtagtggcg ctattttcag acactgattt agctttacgt tctcgtaaaa ttgcccgtat
    15121 cggtgtcaaa tcattaccag acgacttaat tggttattat atggagactt tgccaccacg
    15181 ttttgctgcc tttaatgagg ttcaaaccgg tgagtactta gctattaatg caggcattgt
    15241 ggctatgaac ttggtgttat ccttgacgga tcaaaagatt gcttccaata ttatccttgg
    15301 ttttgataag tcaacgacca atgagatttt agacatcgat ccgcgttttc gtccagaatt
    15361 attgattact gttggctata gtgacgagaa accagaacca agttatcgtt tgcctgttga
    15421 tgaagtcatc gaaagaagat agagtcagga caattagtta aatgtaatgt ccagatgcgc
    15481 tacggagcga ccactattgt tagactgata ctattttggc ttattttaag ttaaacatta
    15541 tccctaaaac gaataaggag agtataatga caaccattga ttttaaagca gaagttgaca
    15601 aacgtaaaga agccatgtta gcagatttag ttgacctatt acgcatcaat tcagaacgcg
    15661 atgacacatt agctgatgac aaacatccct ttggaccagg accagttaag gctttagagc
    15721 atttcctcgc aatggctgag cgtgatggct acaaaacgcg taatattgat aactatgcag
    15781 gtgactttga atttggtcaa ggtgatgaag tactaggaat ctttggtcac cttgatgttg
    15841 tgccagctgg tagcggttgg gatacagacc cttatgagcc agtgattaaa gacgatcgca
    15901 tctatgcgcg tggatcttcg gatgataagg gaccaaccat ggcgtgctac tacgctctga
    15961 aaatcattaa agaattgggt cttcctgtgt ctaaaaaagt tcgtttcatt gttggtaccg
    16021 atgaagaatc tggctggggg gatatggatt attactttgc ccataacggt ttgaaaaatc
    16081 cagactttgg tttctcacca gatgctgaat ttccaattat caatggtgaa aaaggaaata
    16141 tcaccgaata cctccatttc gctggtgaca acaatggtgc ttttgtgctt catcgcttcc
    16201 aaggtgggtt gcgtgaaaac atggtaccag aatctgcaac agctgtcatc acgtcaccgc
    16261 atgaccttga tgtgctagaa gcagctttgg agcaattttt atcagaacat ggtgttaaag
    16321 gttctatgaa ggctactgat ggtaaaattg aagtaactat tattggtaag tctgctcacg
    16381 ggtcgactcc agaagcgggt gttaatggtg ccaccttgtt ggctaaattc ctgaatcaat
    16441 ttacctttga aggggctgct aaggattatt tatatgtggc aggtgaggtg cttcatgaag
    16501 actttgcggc agaaaaacta ggccttgctt ataccgatga ccgtatggga gccctgagca
    16561 tgaacgcagg tgtcttcacc tttgatagtc aatcagctga caataccatc gccttgaatt
    16621 tccgctatcc aaaaggaact gatgcggcaa ccctaaaagc aggattggaa aaattaccag
    16681 gagtaattaa ggtaagcctt tctgagcacg aacatacacc tcattatgtg ccaatggatg
    16741 atgaattagt agctacctta cttgctgttt acgaaaaaca aacaggtctt aaaggctacg
    16801 aacaagttat cggcggagga acctttgggc gcttgctaga acgtggtgtg gcctttggag
    16861 caatgttccc aggtgatgaa aacactatgc accaagctaa tgaatatatg cctttagaaa
    16921 atatttatcg ctcagcagct atttatgctg aagcaatcta cgaattaatt aaataagatg
    16981 cttatgacag caaaaagagc tggaacactg ttccagctct tttgtgatga ttagagactg
    17041 ttgttattta ccaagacaga attgactaaa gagttgggta atgagttcat caggagcagc
    17101 atcccctgtg atttctccga gaatttccca agttctggtt aaatcaacct gtagtaaatc
    17161 aactggcatg ccaagcgcca agccgtcatt gacagcttct aaactctgga ctgccttttc
    17221 aatcaaagaa atgtggcggg catttgacag gtaagtggca tcttgttcta ctagtccagc
    17281 attatcaaag aagagctggt tgatacgatc ttcgatcaga ttaatgttct gattggttaa
    17341 caccgaaata ggaataaggt ccgctggcaa ctgttccaat tcaatctttt gttccaaatc
    17401 tgttttattc agcaatataa tgcggttact gtcctgactg agatttaaca aagctcggtc
    17461 ttggtcagtc agtttttcag aagcattcag cacaagaaga acaaggtcag cttcttgaag
    17521 agctttttta gaccgttcaa ctccgatttg ttcgaccaga tcatctgtct cacgaatacc
    17581 ggccgtatcg acaagtttta gaggaactcc cttgatatta acgtattctt caatgacatc
    17641 tcgggtggtt cctgcaatat cagtcacaat agccttgtct tctcgcaaca aattattaag
    17701 taagcttgac ttaccgacgt ttggacgacc aataatagct gttgataagc cctcacgcag
    17761 aattttaccg cgtttagctg ttcgaaggag attttccaaa aggctttgga attcttgtgt
    17821 tttctcacgt agcagagcag tggtcatttc ttccacatcg tcgtattcag ggtagtcaat
    17881 attgacttcg acttgagcta aggtgttgag aatttcttgt cgggtgtcat tgataagctg
    17941 ggaaagggag ccatccagtt gcttgactgc aatcgtcata gccttgtctg ttttggcacg
    18001 aataatgtcc atgacagcct cagcttgagt caaatcaact cgcccattta agaaggcacg
    18061 tttggtgaat tcgccaggct ctgccattct agcgccttgg cgaatcaata gttgcaagat
    18121 ttcatttgtc acagcaatac caccgtgcgt attgatttca accacatttt cacgggtaaa
    18181 ggttttgggg gctagcatga ctgacaccat aacttcatcg ataatagttc ctgttttagg
    18241 atcaatgata tggccatagt taatagtgtg tgaggcgact tgctctaagt tctttccctt
    18301 aaaaacagat tgtgcaatgg caagcgcatc tgtccctgat agacgaacaa tgccaatagc
    18361 tccttcacca agaggggttg aaatggcggt aattgtatca aattctttag ttatactcat
    18421 gccctctatt ttaaatcaag tcctagtaga aagcaaggta ttattttgta tagaaaatag
    18481 gactagcacc attttgagtt tagctaatac taaaaaagaa taaatgtaaa aaaatcattt
    18541 ttttaataat ttttattgac aatagtatta taaaagaata taatgatata ggttttgata
    18601 aaaaactgac ctaagacagc aggggagcat gctcataata ttcctgccga ggcacaaaac
    18661 gtgattaaag aaaataacgt atttgtactt tcgtgtctag gtttaggtgc gatttttttg
    18721 tacctagccc aaaaataaaa acggaggtaa aactaatgag tgaagcaatt attgctaaaa
    18781 aagctgaaca agtcgaactt attgctgaga aaatgaaagc agcagcaagt atcgttgttg
    18841 ttgattcacg tggtcttaca gttgatcaag atactgttct tcgtcgttca cttcgtgaaa
    18901 gtggcgttga gttcaaagtt atcaaaaact caattttgac tcgtgcagct gaaaaagcag
    18961 gtcttgatga gttgaaagat gtattcgtag gaccatctgc agtagcattt tctaacgaag
    19021 atgttatcgc accagctaaa gttatcaacg actttactaa aactgctgac gcacttgaaa
    19081 tcaaaggtgg tgcaatcgaa ggcgctgttt cttcaaaaga agaaatccaa gcacttgcaa
    19141 cattgccaaa ccgcgaagga atgctttcta tgcttctttc tgtacttcaa gctccagtcc
    19201 gcaacgttgc atacgctgtc aaagctgttg cagaaaacaa agaaggcgct gcttaagacg
    19261 tctcagagta gcttaaggct acgcttaaca ttttaaatta attatttgga ggaaatcaca
    19321 atggcattga acattgaaaa cattattgct gaaattaaag aagcttcaat ccttgagctt
    19381 aacgatcttg taaaagctat cgaagaagaa tttggtgtaa ctgcagctgc tcctgtagct
    19441 gctgcagctg ctggtggtgc tgaagaagct gctaaagatt cattcgacgt tgaattgaca
    19501 tctgctggcg acaaaaaagt tggcgttatc aaagctgttc gtgaaatcac aggtcttggt
    19561 cttaaagaag ctaaaggtct tgttgatgga gcacctgcta acgttaaaga aggcgttgct
    19621 gctgcagaag ctgaagaaat caaatctaaa cttgaagaag ctggagcaac aatcactctt
    19681 aaataagaag cagatagcaa tttgagagcg aaatcccgat ttatcagtct tttagagctt
    19741 atagcgattt gaagaaactg ataaaatgat ttggtttttt gccaaaaatc ctgccaaaaa
    19801 aatctttggc aggattttta ttttctataa aaattaagct gagtagagtg gttattctat
    19861 ctatatgtgt ctagtaataa tttttttatt ttcaggagga aaatcatgca aatgaatgaa
    19921 cagtcatcac gacaaatggt gattgccatc tatctcattt attccaactc aaaatgatag
    19981 tactccttgg ctgttccaag tcgaaattgt tagtgattaa gaaaacctac gagactatgg
    20041 tttgatcgat gaaggccagc agtctcccag tgaaaaaggg cgtttggcta ataagattta
    20101 tttaggaaag ttggaacatg atttcacccc agtcttaagt tcagacgggg ccagtctcaa
    20161 aaacagacca gccccgtgtt aaattcagcc cctagtgaga ctgaaaagag tgaaactgaa
    20221 ggtagtgaaa ctaaagagag taatttagtc attgaggacg aggaggaaag aaaggagtgc
    20281 actagtgtga aaaagactga gggtcacttt actcgtcaag ttgatcatgt caccaaatat
    20341 gacaaagatt atatttggag tttggttcat tcccaattac gtgaggtggt ttatcacaag
    20401 ctgctagtga cttagtcatg agctattttg aagaacgcta tgcttatgcg ctggaacaca
    20461 tacggttcgc aagaacagct gaagctattg cggaatatgt ctttaatggt gtcttatccg
    20521 aatggaccaa gcaactaagg tgacaagaag cgaagtgagt aagatgtttt ggtggctatt
    20581 attaggtgtt tggggactaa ggacaatttg gttgattatt gaagttatca ctgcaccaga
    20641 aatggaagat tatagttgag taaggacgtt acataagcag taaggtcctt ttattttgtc
    20701 agaaaggaat gagagatatg cagtttttag atttgtttgc aggaattgga ggctttcgat
    20761 tgggtttaat taatcagtgc catgagtgta ttggcttttg tgagattgat aagtttgcga
    20821 ggcaatctta caaagccatc tatgagaccg aaggagaaat agaatttcat gacattagac
    20881 aagtcacaga ccaagacttt agacaactta gagggcaagt ggatatcatc tgcggtggtt
    20941 tttcccttgt caagcatttt cactcgcagg cagacgactg ggatttgagg gcactaggag
    21001 aactctcttc tttgagatta ctcgagcgac caaacagata aaaatcatac ccataatcac
    21061 atccaatcaa cgccattgac cgtaattcag ataaaaaatt gatatggaat tatgccttgg
    21121 aacgaaacct ccgtatgatt tcagaccgta tttcaaaaat gactggagca aaaattatcg
    21181 agaagcgtta ttcctaccgt gactatcaaa aatataggca gtctagtcat aaatttgaat
    21241 tgaaacaacg tctttatttt ttgatgcagc agtcaaagtt ctttgatgat tttttagaaa
    21301 aagcagagta attacatgtt catattgatt ttagtcagaa gcatagcaga ttcatgatga
    21361 cggatcgagt aatgaaaaaa ccaattcgag gccgtcaact cagtaaacga gatttatatg
    21421 atgaagaatt ttttcgtaca cattttgcca agatagaaat tgaaagtcga ttagaatttt
    21481 tgttgaaccg tgccaattca ttagaggagc tactattaaa agcaaaagaa gtgaatctaa
    21541 ccattgactt aaaacaaaaa atgtaacttt tatcctagaa gaagatactc aaaagataag
    21601 tttaggt
//
LOCUS       DAUGHY010000032        19915 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.032, whole genome shotgun sequence.
ACCESSION   DAUGHY010000032 DAUGHY010000000
VERSION     DAUGHY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19915
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.032"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(241..390)
                     /gene="rpmG"
                     /locus_tag="VH752_001170"
     CDS             complement(241..390)
                     /gene="rpmG"
                     /locus_tag="VH752_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ8289528.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(406..588)
                     /gene="rpmF"
                     /locus_tag="VH752_001171"
     CDS             complement(406..588)
                     /gene="rpmF"
                     /locus_tag="VH752_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEQ8289529.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            808..2088
                     /locus_tag="VH752_001172"
     CDS             808..2088
                     /locus_tag="VH752_001172"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEQ8289530.1"
                     /translation="MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLLEKLGIKD
                     VTLHLNSLGSPESRSAYRQTLIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVKAMLEALDIPYVIDTNMVRGLDYYNHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGLALPIETEMDVYLA
                     VLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQEVEVSFEDMMTNFANISEQLLS"
     gene            2426..4174
                     /gene="aspS"
                     /locus_tag="VH752_001173"
     CDS             2426..4174
                     /gene="aspS"
                     /locus_tag="VH752_001173"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEQ8289531.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIATVMKETKEIDVTLPFPRMSY
                     DVAMNSYGSDKPDTRFEMLLQDLTVTVKGIDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLTAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANAQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            4167..5108
                     /locus_tag="VH752_001174"
     CDS             4167..5108
                     /locus_tag="VH752_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ8289532.1"
                     /translation="MIKKTTYKKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDFPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIIPQVTLTTDPLINAIFGGLVMGMGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIVTSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            5128..6006
                     /locus_tag="VH752_001175"
     CDS             5128..6006
                     /locus_tag="VH752_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ8289533.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVTGILSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGIIGGLGGGLVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     DELGRGVTYLTGEGAYSGRRKKVIYVALGRRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            6049..6921
                     /locus_tag="VH752_001176"
     CDS             6049..6921
                     /locus_tag="VH752_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ8289534.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILHAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(6948..7256)
                     /locus_tag="VH752_001177"
     CDS             complement(6948..7256)
                     /locus_tag="VH752_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ8289535.1"
                     /translation="MPSEKEILDALSKVYSEQVIQADDYFRQAIFELASQLEKEGMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(7344..9035)
                     /locus_tag="VH752_001178"
     CDS             complement(7344..9035)
                     /locus_tag="VH752_001178"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEQ8289536.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKAKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLAHIFAKMGYKPVKINHLGD
                     WGKQFGMLIVAYKKWGDEAAVQAHPIDELLKLYVRINAEAETDPTVDEEAREWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYDQLHVTFDSYNGEAFYNDKMDEVLDLLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLKEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKEAVAHAVGVGAIKFYDLKTDRMNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDDNSERDNRLALCYATATVLKEALRLLGVDAPNEM"
     gene            9326..9763
                     /gene="argR"
                     /locus_tag="VH752_001179"
     CDS             9326..9763
                     /gene="argR"
                     /locus_tag="VH752_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ8289537.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVATPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            10103..12658
                     /gene="mutS"
                     /locus_tag="VH752_001180"
     CDS             10103..12658
                     /gene="mutS"
                     /locus_tag="VH752_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEQ8289538.1"
                     /translation="MTKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAVDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRELSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGYTLAQVPYIKAILESFDSPCVDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRTGFDERLDHYRKVMREGTGWIADIEAKERQESGINNL
                     KIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKTLATVDVLQSLAVVAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTKLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPKSLLKRADEVLTRLETQSRSTEIMSVPP
                     QVESSSAVRQGQLSLFGDDEKAHEIRQALEAIDVMNMTPFQAMTTLYELKKLL"
     gene            12787..14769
                     /gene="mutL"
                     /locus_tag="VH752_001181"
     CDS             12787..14769
                     /gene="mutL"
                     /locus_tag="VH752_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEQ8289539.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLPLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTGDLRQAIAGIYGLNTT
                     KKMLVISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQSRGLYYDPQKNDFFVKESAVSEKIPETDFYSGAV
                     DNSVKVEKAELLPHSEEVIGPSSVKHASRPQNTFTETDHPNLDLKNRQKLSQMLNRLE
                     NEEQSVFPELDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGDVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            14780..16000
                     /locus_tag="VH752_001182"
     CDS             14780..16000
                     /locus_tag="VH752_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ8289540.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFLTILANLPNAAIPW
                     LTFAGILLVEIASSFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFGVAYYYFDETRPETHAFDHGKGLLASFQNYRQVFQDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLAFTFTQFYAIWLSVVLLTFGEMINVP
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFVVIALL
                     GIYFTVVSAKMKKV"
     gene            16002..16598
                     /gene="ruvA"
                     /locus_tag="VH752_001183"
     CDS             16002..16598
                     /gene="ruvA"
                     /locus_tag="VH752_001183"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEQ8289541.1"
                     /translation="MYDYIKGQLTKITAKYIVVETNGLGYIINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLAGKFVEAPQETGNTKARSNKAGNTQLDEAIEAL
                     LALGYKATELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            16608..17168
                     /locus_tag="VH752_001184"
     CDS             16608..17168
                     /locus_tag="VH752_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEQ8289542.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLDDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVATFTSEEIADALENPSIIRHKLKLAATVNNAIA
                     VQKIQKEFGSFSTYLWNFVGGKPINNLVNQENLVPSQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            17237..18508
                     /locus_tag="VH752_001185"
     CDS             17237..18508
                     /locus_tag="VH752_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEQ8289543.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQTLAKYLRKDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKSM
                     VNEELVPLLSKQYSTLYSKVLRFFGVGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKY
                     DKTITAAESLTAGLFQSTLASFPGASQVFNGGFVAYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVRYIATLHAFNMVRKTLLKLENLL"
     gene            18597..19733
                     /gene="recA"
                     /locus_tag="VH752_001186"
     CDS             18597..19733
                     /gene="recA"
                     /locus_tag="VH752_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEQ8289544.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLAEHPELFD
                     EIDRKVRVKFGLLEESEEESAMAVASEETDDLALDLDNGIEIED"
BASE COUNT         5872 a         3426 c         4416 g         6201 t
ORIGIN      
        1 agatgtcaac ttaaaatcca ccaaaatata gataataagt tagtgtacca aatattaaaa
       61 agccctgcca tcgaaatgat agcagggctt aacttcaata tccagatgat atatttatct
      121 aaaaaaggta gagtaaaagg tagagttttt attgatttgt agtgcgatat aatgagtttc
      181 aaaaaatcaa aaatcgctta aaatcaatat tttgacgtct attgacgtgt actgaaaccc
      241 ttacttaacc tcagtaaaaa caacgtgttt acgaagtttt ggtgagtatt ttttcaattg
      301 aagacggtct ggagtgttac gtttgttttt tgaagtaagg tacaagcgtt caccagattc
      361 tttatgttca agtgtaatat ttacgcgcat ggtatctccc ttctattatt tagcttcgtt
      421 agctttagcg attttacgtc ctttgtagta tcctttgagt gatacacggt gtgaacgtga
      481 gtaatctcca gtagtttcgt cgaattgtac agatggagct gtcaatttgt agtgtgtacg
      541 acgtttgttt ttcttcgctt ttgaagtgtg acgtgcaggt actgccattt tcttattctt
      601 cctttcgatg tgtaagtttt gattttcatc aacttcacta tgataacata tcttttttgt
      661 aaagtaaagt tacttgacaa aactttttat ttttacaatt tcatggtgaa aatactatca
      721 aactaaagca aatgatatat gcttgtttaa aatataagaa aaaatggtag aatgtttaaa
      781 gagaaaacta atcgaagagg taaacttatg aaacttcaaa aaccaaaagg gacccaggat
      841 attctgcctg gggatgctgc gaaatggcag tatgtggaat ctgttgcgcg tgatacgttt
      901 agccaatata attatggaga aattcgtact cctatgtttg aacactacga ggtaatctcg
      961 cggtctgtag gtgatacgac tgatattgtg acgaaggaaa tgtatgattt ctatgataaa
     1021 ggagatcgtc acattacctt gcgaccagag ggtacagctc cagtggtgcg ttcttatgtt
     1081 gaaaataaat tgtttgcgcc agaggtccaa aaacccgtca aactttatta tatcggttcc
     1141 atgtttcgct acgaacgtcc tcaagctggt cgtttacgtg aatttcacca gattggggta
     1201 gaatgtttcg gtgctgctaa tccagcaaca gatgttgaaa ccattgcgat ggcctatcac
     1261 ctccttgaaa aattgggtat taaggatgtc acccttcatt taaatagttt ggggagccct
     1321 gagagtcgtt cagcttatcg tcaaaccttg attgactact taacaccaat gcgtgatcaa
     1381 ttatctaaag atagtcaacg tcgcttggat gaaaacccac tgcgcgtgct tgattctaaa
     1441 gaaaaagaag ataaactagc cgttgaaaag gcgccttcta ttttggatta cttagatgag
     1501 gaaagtcagg cgcactttga ggcagtcaaa gctatgcttg aagcacttga cattccttat
     1561 gtgattgaca ccaatatggt gcgtggcctt gattactata accacactat ttttgaattc
     1621 atcacgtctg tggagggttc tgacttaact atctgtgcgg gtggacgtta tgatagtttg
     1681 gtaggttatt ttggtggacc agaaacccct ggctttggct ttggtcttgg tttagaacgt
     1741 ctcttgatga ttattgaaaa gcagggtctt gccttaccaa ttgagacaga gatggatgtt
     1801 taccttgctg tgctaggtga tggtgctaat agcaaagctt tagaattggt tcaagccatt
     1861 cgccgtcaag ggtttacagc tgaacgagat taccttggcc gtaagattaa ggctcagttt
     1921 aaatcagccg atacctttaa agcaaaattg gtaatgactt taggagaaag tgaagttgaa
     1981 gcaggcaaag ctgtgattaa gaataatcgt agtcgtcaag aagtggaagt cagttttgaa
     2041 gatatgatga caaactttgc gaacatctca gagcaattat tatcttaatg attttattgt
     2101 cgtcataagc ttgtgttgat atgattgttt agaagataat cactaaaaca ggtgtaactt
     2161 ctctttttag aagggagttg cacctgtttg ttttctgtaa ggggattgat tagctttctt
     2221 agtattctgt gaggacacct gatatgtcaa aatgctgacg actcagtatt cttgctttat
     2281 ctttagaaaa actcttttta aatgggtggg gtttttaagg gctttttgct ataatagtag
     2341 ggaatgaatt aattgattta tatagtgaaa ggaattggaa acgagcaggt ttcacaggat
     2401 tctgtttgtt ttagggaatt tatttatgaa acgtagcatg tatgcagggc gtgttcgcga
     2461 agaacacatt ggaacgacaa tcacattgaa aggatgggtc agtcgtcgtc gtgacttggg
     2521 tggtctcatc tttattgatt tacgtgaccg tgagggtgtg atgcaattag tcattaaccc
     2581 tgaggaagtt tccagtgatg tgatggcaac tgctgaacgc ctacgtagtg aatatgtgat
     2641 tgaagtggaa ggttttgttg aagctcgaca acaagccaat gataagttgg cgacaggaat
     2701 ggttgagtta aaagtatctg ctttgaccat tttaaacaca gctaaaacga cgccttttga
     2761 aattaaagat gacgttgaag taagtgatga cacgcgttta cgttaccgtt acttagattt
     2821 acgacgtcca gaaatgttag aaaacttcaa gctacgtgca aaagtgactc attctatccg
     2881 taattacctt gatgaccttg agtttattga tgtcgagaca cctatgttga caaaatcaac
     2941 tccagaaggt gcgcgtgact atctggtacc aagtcgtgtt agccaagggc atttctatgc
     3001 tttgccacaa agtccgcaaa ttaccaaaca gttattgatg aatgctggct ttgatcgcta
     3061 ttatcaaatt gtgaaatgtt tccgcgatga agatctacgt ggtgaccgtc aacctgagtt
     3121 tacacaagtt gacttggaaa cgtcattcct ttctgaacaa gagattcaag acatcgttga
     3181 aggcatgatt gctacagtga tgaaagagac aaaagaaata gatgtaaccc taccattccc
     3241 acgtatgagc tacgatgtag caatgaatag ctacggctct gataaaccag acacacgttt
     3301 tgagatgctt ttacaagact tgacagtaac tgtcaaggga attgacttca aggttttctc
     3361 agaagcacca gctgtcaagg cgattgtggt gaagggaaat gcagatcgat actctcgtaa
     3421 agatattgac aaattaactg agtttgccaa acaatttggt gctaaaggcc ttgcctgggt
     3481 taaggtgaca gatggtcaat tggcaggccc agtagccaag ttcttgacag ctattgagac
     3541 agagttgtca agccaattaa aacttgctga gaatgattta gttttatttg tagcggatac
     3601 tcttgaggtt gctaataata cattaggtgc ccttcgtaat cgtattgcca aggaccttga
     3661 catgattgat cagtcgcagt ttaatttcct ttgggtggtt gattggccaa tgtttgagtg
     3721 gtcagaagaa gaaggacgtt atatgtcagc tcatcaccca ttcactcttc caacgccaga
     3781 atctgctcat gagttagagg gtgatttggc taaggtacgg gctattgctt acgacatcgt
     3841 tttaaatggt tacgagcttg gtggtggtag cctgcgtatc aatcaaaaag agatgcaaga
     3901 gcgcatgttc aaggctcttg gctttaccgc tgatgaagct aacgcccaat ttggtttctt
     3961 actagaagcg atggactacg gctttcctcc tcatggtggc ttggcaattg gtcttgaccg
     4021 ttttgtcatg ttgcttgctg gaaaagacaa tattcgagaa gttattgcct tccctaaaaa
     4081 taacaaagcc tctgatccaa tgacgcaagc acctagtctt gtttctgaaa accaattaga
     4141 agaactcagt cttcaaatag aaagtcatga ttaagaaaac aacctataag aaaaaagtta
     4201 aatatgtcat tagtagaggg gccaagaaag ttggcctact ccacgctcta agaagtattt
     4261 caagagaaaa atatgcagag aagatttcgg cttctctgct ttatggcatt ctctctagta
     4321 ttgctgtgaa ttttttcttc cagcctgggc atgtttattc aagtggagca actggtctag
     4381 cacaggtttt ttcagctctt agtcatcgtc ttttaggcta tgattttccc atcgcctttg
     4441 cgttttattt gattaatatt cctttgctta ttttagcttg gtataaaatt gggcatcaat
     4501 ttaccatttt tacctttatc acagtcagca tgagttcttt ctttattcaa atcatacctc
     4561 aagtgacgct gacgactgat cctcttatca atgctatttt tggtggtttg gttatgggaa
     4621 tgggaattgg tacaggtctc aaatcacgta tctctagtgg ggggactgat attgtcagtt
     4681 tgacccttag aaaacgaaca ggcaaggatg tgggcagtct ctcattgatg gttaatggtg
     4741 caattttagc ctttgcaggg attttatttg gctggcagta cgccctttat tctatggtct
     4801 ctatctttgt atcaagtcgt gttacggatg ccattttcac caagcaaaag aaaatgcagg
     4861 caactattgt taccagccat ccagagcgtg tgattcatat gatccataaa cgtctgcatc
     4921 gcggggtgac cagtatcaac gacgcagaag ggacttacaa gcatgaacaa aaagcagttt
     4981 tgattaccat tttgacatgt gaagaatacc cagaattcaa atggctgatg ttaaaaacag
     5041 acccacaagc ctttgtttca gtggctgaga atgttagaat tatcggtcgt tttgtggaag
     5101 atgattaaga aaaacacagg aggtgtcatg tcaattgacc aacgtttaca gttaaagaaa
     5161 ttttgcttta ttattttagg agctgctatc tacgcctttg cttttgttta tttttacatg
     5221 gctaatcgca ttgcagccaa tggtttagct ggtctcaccc tagttggtaa ggcacttttt
     5281 gagattgatc cttctttgat aggctatttg attaatttgc ctttggtctt gctgggagct
     5341 agatgttttg ggaaaagagc catggtttac acagtaacgg gtattttatc actttacttt
     5401 tttgtgtggc tgtttcaacg ctttcctttg gtcgttgact tggaccatga caatttagtc
     5461 gtttccttga tggctggaat tattggtggt ctaggtggcg gtcttgtgtt tcgcaatggt
     5521 ggaaccattg gtggagcaga tattattgct aagttattag aagacaagtt aggactacag
     5581 ctgaatcaag ctcttttagg cattgatctt tttgtcatga ttgtctcttt gacttatatt
     5641 tccctgccgc aaatgatgta tgctctgatt gctagcttta tgtatggtca aattgttcgc
     5701 ttggtacaac aaggtggtta ttcagcccgt ggcattttta tcgtttcaga acaagcggag
     5761 aaaattgccc ggttcatcat ggacgaattg ggaagaggag ttacttatct aacaggtgaa
     5821 ggagcctact ctggtcgccg taaaaaagtg atttatgtgg ctttggggcg aagagatatt
     5881 agggaattaa aagctttttt aactcaggtt gatcccaatg cctttatcac ttattttgat
     5941 gttaatgaag tcaatagccc agaattttta actattaaaa gcaaatacca taaaaagaga
     6001 aaataaatga gcattagagt tgcctgcgaa gaatagtgag gataaaggat ggcacatcac
     6061 gataaattga ctaaactact aaaactgttt ttgattgccc taggtgtagc catttatacc
     6121 ttcggttttg ttaattttaa catggctaat gctttggctg aaggtggtgt ggcaggaata
     6181 accttgattt tacacgcgca ttttgggatt aatcctgctt actcttctct cttatttaat
     6241 cttccacttt ttattttagg ggcaaagatt tttgggaagc gttctttggc tctaaccatc
     6301 tatggaacag ttctgatgtc cgcttttatc tggatgtggc aaaaagttcc tatcgaactt
     6361 ggcttggaaa atgacatgat gttggtggct gtagtggcag gtctcttttc agggattggg
     6421 agtggcattg tttttcgcta tggtgcaacc acaggtggaa cggatattat tggtcgcatt
     6481 gcagaagaaa aatttggagc taagctaggt caaactttgc ttttggtgga tgctcttgta
     6541 ttgacagctt cgttgactta tgtggattta aaacacatgc tttatacttt ggtggcaagc
     6601 tttgttttta gccaaatgat tagtgtggtt caaaatggtg gttacactat ccgtggaatg
     6661 attattatta ccaagcattc agaggctgcc gctcaagcta tcctaaccga aatcaatcgt
     6721 ggagtgactt acttgaaagg tcaaggagct tactctggta atgattacaa tatcatgtat
     6781 gtgaccttga acccaactga agttcgggaa gtcaaacgta ttttagctgg tttggatcca
     6841 gatgccttta tctccattat tgatgtggac gaagttatta gctctgattt taaaattcgc
     6901 cgaagaaatt atgataaata aaaatacagg tcaaacagcc tgtattttta ttttggtccc
     6961 ccacttaaga aactaccaag cataatagct gatatagcag ttcccttgta atggctagct
     7021 tttgttttta ccaagcgact aagatcaaat atgcttttag gagcatcaaa ttgatgtgtt
     7081 aagatgtatt gattgataag tgagtcaatt tttgtagcta ggagtgagct catcccctct
     7141 ttttcaagtt gactggcgag ctcgaagatc gcttgacgaa aataatcatc ggcttgtatt
     7201 acttgttcgc tataaacttt actaagagca tctaatattt ctttttctga tggcatggca
     7261 ttctcctgta acatattgtg ctattattat aatataaaaa actaagactg tcataaatgt
     7321 gacagcctta gtaatgttat tacttacatt tcatttggtg cgtcaacacc gagtaagcga
     7381 agcgcttctt taagaacagt agcagttgca tagcagagag ctaagcggtt atcacgttct
     7441 gagttgtcgt caagaatgcg agtgtgagca taatatttgt taaagctttg ggctaagtta
     7501 atagcaaatt tagccataat agaaggctca aagttatctg atgttcgctt gataatgcgt
     7561 gggaaatctt ggataagttt gataatttcc cagctttcag catcagctaa actgtaagtg
     7621 gtggttgcag atggcgtaaa gtcagcttta cgtagaatag attggatacg agcatgagca
     7681 tattgaacgt aaggtccagt ttctccttca aaggaaacca tggcttcaag gtcaaagtca
     7741 tacccattca tacgatctgt tttgagatcg taaaacttga tggcaccgac accaacagcg
     7801 tgtgcaacgg cttctttgtc agcaaggttt ggatttttcg cttcaatttg agaagctgca
     7861 cggttaatgg cttcagcaac cgtaggttca agaagaataa cattaccttt acgtgttgat
     7921 aatttagcac cgccttttgt gacaagtcca aaagcaacgt gtgtcatatc atctgaccaa
     7981 tcgtaaccca tttcttttag cactgctttt aattgtttga agtgagcagc ttgctcatta
     8041 ccaacaacat aaacggattt agcaaagtca taagtgcgct tacggtaaag tgcggcagct
     8101 aggtcacggg taatgtaaag agtagcgcca tctgattttt tgatcaaggc agggtgttca
     8161 atgccgtatt tttcaaggtt aacaacttgg gcacctttag attctacaag aaggttttta
     8221 gcttctaata agtcaagaac ctcatccatt ttgtcattat agaaggcctc accgttgtaa
     8281 ctatcgaagg tgacatgtaa ttggtcataa agtcggttga actcaagtaa actttcatca
     8341 cggaaccatt gccataattc agtagcttct ttgtcgccat cttccaattt acggaaccat
     8401 tcacgagctt cttcatcaac ggtgggatca gtttcagctt cagcattgat acgaacgtaa
     8461 agttttaaga gttcatcaat aggatgtgcc tgaacagcag cttcatctcc ccattttttg
     8521 taggcaacaa tgagcatacc aaattgttta ccccagtcac caaggtgatt gatttttaca
     8581 ggtttatagc ccattttggc aaaaatgtga gcgagactat caccaataac tgttgagcgg
     8641 aggtgaccga ttgaaaatgg cttagcgatg tttgggcttg acatgtcaat agcaacattt
     8701 cgcccttgac cttcgtcttg ctgagcatag tctgaacctg ctgtaatcac ttgttcaagg
     8761 acctgtgagg aaatcttagc tttatcaagg aaaaagttga tgtaagggcc gacagctacg
     8821 actttttcaa attggctctc atcaatctgt tcagctaact cgctagcaat catttgaggg
     8881 gctttgcgaa ggacttttgc taaactaaag gctggaaagg caaggtctcc catgtcagaa
     8941 ttttttgggg tttcaagcaa gttaaaaata gcatcttgtt ctaattctgg aacaacttta
     9001 gctatttcac ttgcgattag agttttagta tccatacagc gtctcctaaa aattgtttgt
     9061 caccctattc taacatattt ctttaaaata aacgaggaga ggattgataa attatactga
     9121 aactttcaag gaaatgaatt gcttgaggtt gttgtttttc tgaaaatgtt ataaaaagag
     9181 aaatgaggtt tcttttcaac agttttcaag tgaaattcta gttgaatttg ataaaaaatt
     9241 gctgaaaata gctgggcgaa gatgaaataa tacacttttt ttgttataat ttgagaggat
     9301 aattatacaa ttggggtgat atatcatgaa taaaatggag cgtcaacagc agattaagcg
     9361 aatcattcaa gcagagcata ttggcacaca agaagatatt aaaaatcacc ttcaaaaaga
     9421 aggcattgtg gtcacccaag caactctatc tcgggactta agagaaattg gccttttgaa
     9481 attgagagat gaacaaggca aactctatta tagtttgtct gaacccgttg caacaccatt
     9541 tagtcctgag gtgcgttttt atgttttgaa agttgatcga gcaggtttta tgctggttct
     9601 ccataccaat ttaggagagg ctgatgttct agctaatttg attgacaatg atgccattga
     9661 agatattcta ggaaccattg ctggtgcgga taccttgctt gtgatttgca gagacgaaga
     9721 gattgctaaa cgctttgaaa aagatttggc tgcaggccta tgactagcta tcaagagtct
     9781 ttgacacaat tgattcttgc tatccatgaa ttctagtatc gttgcttata aagaagtgaa
     9841 gcaatagctg tctaagctcc atgattttaa aaagtaagct tatcagatga aacgctatca
     9901 acaagatgcc ttgcttttca aaaaaaaata gataaagaaa aggctgcgac agtatctgca
     9961 agcagggcaa aagaactaga agataggctc agtcatcagc cattattaat tgatgattat
    10021 cgagaaaaga tgcaagatgc aagtgatgtg attcagtata tcaccaaacg tatagaagat
    10081 cagttaaaca aggagttaac aaatgacaaa aactaacatt tctcctggaa tgcaacagta
    10141 tctggacatc aaaaaagatt atccagatgc ttttttgctt tttaggatgg gtgactttta
    10201 tgaattattt tacgaggacg ctgtcaaagc agcacaactc ttagaaattg gtttgaccag
    10261 tcgcaacaag aatgcggaaa atccaattcc catggcaggc gtgccacatc attctgccca
    10321 acaatacatt gatgtgttaa ttgagttggg ttacaaggtt gctgtcgcag aacaaatgga
    10381 agacccaaag caagctgttg gggtggtgaa gcgtgaggtc gttcaagtca taactcctgg
    10441 aacggttgtg gattcagcta agccagatag cgccaataac tttttggtag ctgttgactt
    10501 tgatggttgc cgttatggat tggcttatat ggatgtatcc acaggtgaat tttgcgtgac
    10561 agatttggcg gactttacga gtgttcgtag cgaaatccaa aacctcaagg caaaagaagt
    10621 cttactaggt tttgatttat ctgaagaaga acagacgatt ttggtcaagc agatgaattt
    10681 gctgctttct tatgaagaaa cggtctatga agataaatct ttaattgacg gccaattgac
    10741 aacggtagaa ctgacagcgg caggaaaact cttgcaatac gttcacaaaa cacaaatgcg
    10801 agaactcagc cacttgcaag ccttggttca ctatgagatc aaggattatt tgcagatgtc
    10861 gtatgccact aagtcaagtt tagatttggt agaaaatgct aggactaata aaaaacatgg
    10921 aagtctctat tggctgttag atgaaaccaa gacagctatg gggatgaggc ttttgcgctc
    10981 atggattgat cgacctttgg tttctaaaga agctatttta gagcgtcaag aaattattca
    11041 agtttttctg aatgctttta ttgagcgaac cgatttaagc aatagtttaa aaggtgttta
    11101 cgacatcgaa cgcttatcta gtcgcgtgtc ttttggcaag gcaaatccga aagatttact
    11161 tcaattgggg tataccttag cccaagtgcc ttatatcaaa gctatcttag agtcttttga
    11221 cagtccttgt gttgacaaac ttgtcaatga tattgacagt ttgcctgagt tggaatactt
    11281 gattagaaca gccattgatc cagatgcacc agcaactatt agtgaaggca gtattattcg
    11341 tactggtttt gatgagcgct tggaccatta tcgtaaagtg atgcgagaag gaacaggttg
    11401 gattgcggat attgaggcca aagagcgtca agaaagtggc attaataacc taaaaattga
    11461 ttacaataaa aaagatggtt attattttca cgttacgaat tcaaatctta gcttagttcc
    11521 cgagcatttt ttcagaaagg caactttaaa aaattctgag cgttatggaa cagcagaatt
    11581 ggctaagatt gaaggtcaga tgttagaggc tagggaagag tcatctagtt tagaatacga
    11641 tatttttatg tgtattcgag ctcaagttga aacctatatt aatcgtttac agaaactggc
    11701 taaaactttg gcaacggtgg atgttttgca aagtttagca gtcgttgctg aaaccaatca
    11761 ttatattcgg ccgcagttca atgataatca tgtgattaca attcaagaag gtcgtcacgc
    11821 cgttgttgaa aaggttatgg gagtgcagga atacattccc aatagtatct cttttgacca
    11881 acagaccagt attcagctga ttacaggtcc aaatatgagt ggtaagtcga cttatatgag
    11941 acagctggcc ttaacggtta tcatggccca gatgggttca tttgtggctg ctgatcatgt
    12001 tgatttacct ttatttgatg cgatttttac gcgtattggg gctgctgatg atttgatttc
    12061 tgggcaatca acctttatgg tggagatgat ggaagcaaac caagcaatca aacgcgcaag
    12121 tgacaactct cttattctat ttgatgaact gggacgaggt acggcaactt atgatggtat
    12181 ggctttagcc caggcaatta ttgaatatat ccatgataga gttggtgcta agaccatatt
    12241 tgcaacgcat tatcatgaat tgacagattt gtcaactaag ttgacaagtc tagtcaatgt
    12301 tcatgtagca acgcttgaaa aagatggcga tgttaccttc cttcataaga ttgctgaggg
    12361 accggcggat aaatcttacg gtattcatgt tgcaaaaata gcaggactgc caaaatccct
    12421 attaaagaga gcagacgaag ttctgacccg tttagaaaca cagtcacgat ctactgagat
    12481 aatgtcagtc cctccacaag ttgagtcaag cagcgctgtt agacaggggc aattatccct
    12541 ttttggtgat gatgagaaag ctcatgagat taggcaagca ctggaagcta ttgatgtcat
    12601 gaacatgacc ccgtttcaag caatgacaac tctttacgaa ttgaaaaagt tgttatagtc
    12661 tttcagctga aaatgaaaaa gatgctttct atattgaagg catctttttg ttctgtcaaa
    12721 aacggtccga ggccttcggc tatttattaa gtgtgttata atagtccata agaatgcgag
    12781 gaaattatga caaacattat tgaattaccg gaagttctcg ccaaccaaat tgcagctggt
    12841 gaagttgtag aaaggccagc gagtgttgtc aaggaattgg ttgagaatgc tattgatgct
    12901 aaaagtagcc agattaccgt tgaaattgaa gagtctggcc ttaagatgat acaggttaca
    12961 gacaacggtg agggaatgtc tcatgaagat ttacctttaa gtctgcgtcg ccacgctact
    13021 agtaaaatta agagtcagag tgatttgttt agaattagaa cacttggctt ccgtggagag
    13081 gctttaccgt ctgttgcctc tatcagtaaa atcacgataa aaacagcaac aaaagaagtc
    13141 actcacggtt ctcttcttat agctactggt ggggaaattg agacacttga agcgatctca
    13201 actcctactg gaaccaaaat taaggttgaa aacctttttt acaacacgcc tgctcgtctc
    13261 aaatatatga aaagtttaca ggcagaatta gctcacattg tagatgtggt caaccggttg
    13321 agtttggcac atccagaagt tgctttcaca ctgattagtg atggtcgcca attaactcag
    13381 acatcaggaa ctggcgattt acgccaagca attgcaggga tttatggttt aaatactacc
    13441 aaaaaaatgc tggttatctc taatgctgat ttggattttg aagtttccgg ctatgttagc
    13501 ttgccagagt tgacacgcgc caaccgtaat tacatgacga ttttggttaa tgggcgttac
    13561 atcaagaatt tcttgcttaa tcgagcaatt cttgatggtt acggttctaa gctcatggtt
    13621 ggacgtttcc caattgttgt gattgatatt cagattgatc cctatttggc cgatgtcaat
    13681 gttcatccca caaaacaaga ggttcgtatt tcaaaagagc gtgaattaat ggctttaatt
    13741 agcacggcta tttcagagag tcttaaggaa caagatttga ttccagatgc tctggaaaac
    13801 ttagccaaat caagcacacg acatttctct aaaccagagc aaacacaact tcctttacag
    13861 tctagggggc tttattatga tcctcaaaag aatgactttt ttgtcaaaga gtcggctgtc
    13921 tcggaaaaaa tacctgaaac tgatttttat tccggcgctg ttgacaacag tgtaaaggtt
    13981 gaaaaagcag agctgttacc ccactcagaa gaagttatag gaccttcttc ggttaaacac
    14041 gcaagtcgtc cccagaatac ctttaccgag actgatcatc ctaatcttga cttaaaaaac
    14101 agacaaaagt tatctcaaat gttgaatcgc ttggaaaatg aagagcaatc agtgtttccc
    14161 gaattggatt attttggtca gatgcacgga acttatctct ttgctcaggg aaaagatggt
    14221 ttatttataa ttgaccaaca cgctgcccag gagcgggtta aatacgaata ttatcgtgat
    14281 aagattggcg acgttgacag tagtttacag caattgttgg tgccctattt gtttgagttt
    14341 tctggttctg atttcattaa cttacaggag aaaatggcac tcttaaatga agtcggtatc
    14401 ttcttagaag tttatgggca caatactttc attttgaggg agcatcctat ctggatgaaa
    14461 gaagaagaga ttgcatctgg tgtctatgaa atgtgtgaca tgttgcttct aaccaatgag
    14521 gtatctatca aaacttaccg agcagagtta gctattatga tgagttgtaa acgatctatc
    14581 aaggcaaatc atagcttgga tgattattca gcaagaaatc tgctactgca attggcccaa
    14641 tgtcaaaatc cttataactg ccctcatggt agacccgtat tgattaattt tagtaaggca
    14701 gatatggaaa aaatgttccg tcgaattcaa gaaaatcaca ctagcctgcg agagctagga
    14761 aaatattaga gaggatgaga tgcaagagtt tttaaacctt cctaagcaga ttcagctgag
    14821 gcaactggta cgctttgtga ccattacctt aggcagtagt atctttccct ttatggccat
    14881 gtattatacg acttactttg gtacgttttg gacaggcctc ttaatgatga ttaccagttt
    14941 gatgggattt gttggaactt tatacggtgg gcatctgtca gatgctcttg gtcgtaaaaa
    15001 agtcattatg attgggtcag taggaacaac gctaggctgg tttctgacta ttttagctaa
    15061 tttgcctaat gcagctattc cttggttaac ctttgcgggt attttattgg tagagattgc
    15121 ttctagtttt tatggtcctg cctatgaagc tatgttgatt gatttgactg atgagagtaa
    15181 tcgtcgattt gtttacacca tcaattattg gtttatcaat attgccgtca tgtttggtgc
    15241 agggctatct gggctttttt atgaccatca ttttttagcc ttgttagtag ccttattact
    15301 cgttaatgta ctttgttttg gcgttgctta ctactatttt gatgagacta gaccagaaac
    15361 acacgctttt gatcatggta aaggattact ggcgagtttt cagaactacc gtcaggtgtt
    15421 tcaggatcgt gcctttgtct tgtttacctt aggtgccatc ttttctggta gtatctggat
    15481 gcagatggat aactatgtgc cagtccattt gaaactgtat tttcagccaa cggctgtgtt
    15541 aggtttccaa gtaactagtt ctaaaatgtt atcattaatg gttttaacta atacattgct
    15601 gattgtcctt ttcatgacag tagtaaataa attaacggaa aaatggaaac tattacctca
    15661 gcttgtggtt ggttctttac tatttactct agggatgctc ttggcattta cctttacgca
    15721 gttctatgct atttggctat cagttgtttt gttaactttt ggggaaatga taaatgttcc
    15781 tgctagtcaa gtcctacgtg ctgatatgat ggatcattcc caaataggat cttatacagg
    15841 ttttgtgtca atggcacaac ccctaggtgc tattttggct agtctactag tatctgtcag
    15901 ccattttaca ggtcctttag gtgtgcaatg cttatttgta gtcattgctt tgctagggat
    15961 ttattttacg gttgtttctg caaaaatgaa aaaggtgtag tatgtacgat tatattaaag
    16021 gtcaattgac caaaattacg gcaaaataca ttgtcgttga aacaaatgga ctaggctata
    16081 ttatcaatgt agccaatcct tatagcttta cagatagtgt caaccaactg gtaactattt
    16141 acctgcatca agtgattcgt gaggatgctc acttgttgtt tgggtttcat acggaagacg
    16201 aaaaagatgt ttttctgaaa ttaatttctg tatctggcat tggccccaca acagctcttg
    16261 ctattgtggc agttgatgat aatgagggac ttgtgaatgc cattgataac agtgacatta
    16321 agtacctaat gaaatttcct aaaattggta aaaaaacagc gcagcaaatg gttcttgact
    16381 tagctggcaa atttgtggag gctccacaag agactggtaa taccaaagcg cgcagcaata
    16441 aagcaggcaa tactcaactg gacgaagcga ttgaagccct ccttgccctc ggttataagg
    16501 caacggaatt gaagaaaatt cgtgccttct ttgaaggaac ctctgagacg gcagagcaat
    16561 acatcaaatc agcattgaaa ctgttaatga aagggtaggt gtttcacatg aaacgttgct
    16621 catgggttcc taaggataac cagctttact gtgattacca cgatttagaa tggggtcaac
    16681 ccctggatga tgatagagat ttttttgaat tgctgtgcct tgaaagttac cagtcaggct
    16741 tatcctggct gactgttttg aaaaaacgcc aagctttcag aactgttttt catcattacg
    16801 acattgcatc ggttgcaacc tttacctcgg aggaaatagc tgatgcttta gagaatccat
    16861 ccatcatccg ccacaagcta aagctagcag caactgtcaa caacgctatc gcagtacaga
    16921 aaattcaaaa agagtttggt agtttttcaa cttatctctg gaattttgtt gggggaaagc
    16981 caattaacaa ccttgtcaat caagagaatc ttgttccttc tcaaacagaa ttatctattc
    17041 gcttagctaa ggatttaaag aaaagaggat ttaaattttt agggcctaca acggtttatt
    17101 cttttatgca agcatcgggt ctggtaaatg accatgaaga ggcttgtgtt ttcaatagaa
    17161 tcttctaatt cttcgtgaat tggaagattt tttgataaaa taagatcaga ttacgaatag
    17221 ataagtaggt ggggaaatga aagctgaact gattgcagta ggtaccgaaa ttttgactgg
    17281 tcaaattgtg aataccaatg ctcaatttct gtcggaaaaa atggcagagc taggtattga
    17341 tgtctatttt caaacggctg ttggggacaa cgaggagcgt ttactttcag tgattacaac
    17401 tgctagtcag cgtagtgact tggtgatttt atgtggtggc cttggtccaa cgaaagatga
    17461 tttaaccaaa caaactttag caaagtacct taggaaagac ttggtttatg atgagcaagc
    17521 ttgtcagaaa ctagatgact tttttgctaa gcgcaagcct tcatcacgga caccaaataa
    17581 tgagcgacag gcacaagtga ttgaagggtc aatccctttg ccaaataaaa ctggtcttgc
    17641 ggttggtggg ttcatcacag tcgatggtat tagttatgtt gtcttaccgg gtcctccaag
    17701 tgaattgaag tcgatggtaa atgaagaatt ggtaccactt ctgtcaaaac aatacagtac
    17761 attgtattca aaggtactac gcttttttgg tgttggggaa agtcagttgg taacagtttt
    17821 gtcagatttt attgagaatc aaactgatcc aactattgct ccgtatgcta agactggcga
    17881 agtgactctt cgcttatcaa caaaaactga aaaccaagct ctggcagata aaaagttagg
    17941 tcagctagaa gcgcagctac tatcccgaaa aactcttgaa ggtcaaccct tagctgatgt
    18001 cttttatggc tatggggagg ataattcctt agcgcgtgag acatttgagc tcttagtaaa
    18061 atatgataag acaattacag cagcagaaag tctaaccgcg ggattatttc agtcaacttt
    18121 ggcgagtttt ccaggagctt ctcaagtatt caatggaggc tttgtggctt atagcatgga
    18181 agaaaaagcg aaaatgctag gccttccttt agaggagttg aaatcgcatg gcgttgttag
    18241 tgcttataca gccgagggga tggcggagca agcaaggtta ttgactggtg ctgatattgg
    18301 ggtaagttta acaggtgttg ccggaccaga tatgttggag gaacagcctg caggtacagt
    18361 tttcattggc cttgccactc aaaataaggt agaatcaata aaggttttga ttagcgggcg
    18421 aagtcgtttg gatgtgcgct atatcgccac tttacatgcc tttaatatgg tccgtaaaac
    18481 tttattaaaa cttgagaatt tgctataatg ttatttagta gctatagtag gatatggttt
    18541 acaattatgt agattaatcc tgctgacgat ggtttgttaa aaaagaggag aaagtattgg
    18601 caaaaaaatt aaaaaagaat gaagaaatca ctaagaagtt tggtgacgaa cgtcgcaaag
    18661 cccttgatga tgctttgaaa aatattgaaa aagattttgg taagggcgca gtgatgcgat
    18721 taggagaacg tgcagagcaa aaagttcagg ttatgagttc aggaagtcta gctcttgata
    18781 ttgcgcttgg agctggtggt tatcctaaag gacgtatcat cgaaatctat ggtccagagt
    18841 cttccggtaa aacgactgtg gctttacatg ctgtagcaca agctcaaaaa gaaggtggaa
    18901 tcgcagcctt tatcgatgcc gagcatgcgc ttgatccagc ttatgctgct gcgcttgggg
    18961 ttaatattga tgaactcctc ttgtctcaac cagattctgg agaacaagga cttgaaattg
    19021 caggtaaatt gattgattct ggtgcggttg atctggttgt tgtcgattca gtagcagctt
    19081 tagtgccacg tgctgaaatt gatggtgata ttggcgatag ccatgtcgga ttgcaagcac
    19141 gtatgatgag tcaggccatg cgtaaattat cagcttctat taataaaaca aaaactatcg
    19201 ctatctttat taaccaattg cgtgaaaaag ttggtgtgat gtttggaaat cctgaaacaa
    19261 caccaggtgg tcgagctttg aaattctatg cttctgttcg gctggatgtg cgtggaacaa
    19321 ctcaaattaa aggaactggt gaccaaaagg atagcagcat tggtaaggag accaaaatca
    19381 aggttgttaa aaacaaggtc gctccgccat ttaaggtagc agaagttgaa atcatgtacg
    19441 gggaaggtat ttctcgtaca ggggagcttg tgaaaattgc ttctgatttg gacattatcc
    19501 aaaaagcagg tgcttggttc tcttataatg gtgagaagat tggccaaggt tctgaaaatg
    19561 ctaagcgtta tttggccgaa catccagaat tgtttgatga aatcgaccgt aaagtacgtg
    19621 ttaaatttgg tttgcttgaa gaaagcgaag aagaatctgc tatggcagta gcatcagaag
    19681 aaaccgatga tcttgcttta gatttagata atggtattga aattgaagat taaaaagagc
    19741 ccttcagggc tttttaaaat gtatttttaa aggttagtag gactactgtt cataggtgtg
    19801 tgggtgaata gaaaccatca tttaaaaaga tgtgagaaag cagaaattag gataactgat
    19861 ataaaattaa aataactgat aattcaaggg aatacaggtg aaagacctta tttta
//
LOCUS       DAUGHY010000033        18132 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.033, whole genome shotgun sequence.
ACCESSION   DAUGHY010000033 DAUGHY010000000
VERSION     DAUGHY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18132
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.033"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(8..1174)
                     /locus_tag="VH752_001187"
     CDS             complement(8..1174)
                     /locus_tag="VH752_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEQ8289545.1"
                     /translation="MENQLGQLALEQAKTFGGKLEVQPKVDIKNKHDLSIAYTPGVAS
                     VSSAIAKDKTLAYDLTTKKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAG
                     VDAIPIVLDTKDTEEIISIVKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHD
                     DQHGTAIVVLAAIFNSLKLLKKSLDEVSIVVNGGGSAGLSITRKLLAAGATKVTVVDK
                     FGIINEQEAAQLAPHHLDIAKVTNREFKSGTLEDALEGADIFIGVSAPGVLKAEWISK
                     MAARPVIFAMANPIPEIYPDEALEAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAAKGIASLVPDDALSTTNIIPDAFKEGVAEIVAKSVRLVVLKS"
     gene            complement(1206..2537)
                     /locus_tag="VH752_001188"
     CDS             complement(1206..2537)
                     /locus_tag="VH752_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEQ8289546.1"
                     /translation="METISKKMPQKDLSEHSKAWQNRRIGSVPLPVYLVLATLILVTG
                     WLQQLPVNMLGGFAVILTLGWLLGTIGATIPGLKHFGGPAILSLLVPSILVFFNLLNP
                     NVLEATNVLMKQANFLYFYIACLVCGSILGMNRKILIQGLFRMIIPMLLGMVCAMGVG
                     TLVGVILGLDWQHTLFYVVTPVLAGGIGEGILPLSLGYSAITGVGSEQLVAQLIPATI
                     IGNFFAILCTALLNRFGEKHPSYSGQGQLVKIGHSENMSDALKDNSGALDVKLMGAGV
                     LTACSLFIAGGLLQHLTGFPGPVLMIILAAFLKYLNVIPQETQNGAKQLYKFISSNFT
                     FPLMAGLGLLYIPLKDVVATLSWQYFIVVISVVLTVVSVGFFVSRFLNMNPVEAAIIS
                     ACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRIIFS"
     gene            2698..4239
                     /locus_tag="VH752_001189"
     CDS             2698..4239
                     /locus_tag="VH752_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8289547.1"
                     /translation="MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETH
                     LLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTHPN
                     PKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
                     LNDVALTSKRNYTLSLLLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLD
                     QIEAAVFVVDKAGILQLCNQAGQKLIARKCQLGKPTGNSFNHLFPDFPKLSLQEGHEQ
                     LFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHK
                     FMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKY
                     QELNVHLKIDVLSEIPHSATKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHL
                     ISHYTLTDNWIDLERVQPIFDLPYFQQLLTDTNSQFHQHQTVTELQFSVTTPYVGGQH
                     ERFNH"
     gene            4220..4885
                     /locus_tag="VH752_001190"
     CDS             4220..4885
                     /locus_tag="VH752_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ8289548.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRAQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIMENIKAFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(4940..6013)
                     /locus_tag="VH752_001191"
     CDS             complement(4940..6013)
                     /locus_tag="VH752_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ8289549.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDDIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(6006..6782)
                     /locus_tag="VH752_001192"
     CDS             complement(6006..6782)
                     /locus_tag="VH752_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289550.1"
                     /translation="MKKFANLYLASVFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGTFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            complement(6779..7573)
                     /locus_tag="VH752_001193"
     CDS             complement(6779..7573)
                     /locus_tag="VH752_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289551.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMVAIMWLTKEKSK"
     gene            complement(7557..8711)
                     /locus_tag="VH752_001194"
     CDS             complement(7557..8711)
                     /locus_tag="VH752_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289552.1"
                     /translation="MTEPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKEFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEDGLINAIEEERNEENL"
     gene            complement(8757..9644)
                     /gene="murB"
                     /locus_tag="VH752_001195"
     CDS             complement(8757..9644)
                     /gene="murB"
                     /locus_tag="VH752_001195"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEQ8289553.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     AYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKLNAVHLNGYTLKAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTPSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHAGFMINVADGTAKDYEDLIAYVIETV
                     ENHSGVKLEPEVRIIGENL"
     gene            complement(9794..10294)
                     /gene="folK"
                     /locus_tag="VH752_001196"
     CDS             complement(9794..10294)
                     /gene="folK"
                     /locus_tag="VH752_001196"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEQ8289554.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEVYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            complement(10291..10650)
                     /gene="folB"
                     /locus_tag="VH752_001197"
     CDS             complement(10291..10650)
                     /gene="folB"
                     /locus_tag="VH752_001197"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEQ8289555.1"
                     /translation="MDKIVLEGCRFYGYHGAFKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGEKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            complement(10657..11457)
                     /gene="folP"
                     /locus_tag="VH752_001198"
     CDS             complement(10657..11457)
                     /gene="folP"
                     /locus_tag="VH752_001198"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEQ8289556.1"
                     /translation="MKIGKFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESTRPGCQFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWVDPGFGFAKSVQQNTELLKGLDRVCHLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLSQLM"
     gene            complement(11466..12050)
                     /gene="folE"
                     /locus_tag="VH752_001199"
     CDS             complement(11466..12050)
                     /gene="folE"
                     /locus_tag="VH752_001199"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEQ8289557.1"
                     /translation="MKRERLMSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKM
                     YAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSD
                     GRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRG
                     IKKPGSKTITTTARGLYKESRAERQEVISLMTKD"
     gene            complement(12085..13362)
                     /locus_tag="VH752_001200"
     CDS             complement(12085..13362)
                     /locus_tag="VH752_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ8289558.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYP
                     RLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILF
                     GAIDTKPIADMLVALEQIGDLQVTGFHYPNAYPLEKYPERFGRVADFKDFLALRKHAK
                     ADDFFVITGSLYFISEIRQYWKKHIEKNVLLTH"
     gene            13694..14656
                     /locus_tag="VH752_001201"
     CDS             13694..14656
                     /locus_tag="VH752_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ8289559.1"
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGL
                     SDVSQFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPIL
                     MYHAIHVMSPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKK
                     VVWLTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEIGSAANLTLKQMKEMKQFGM
                     SFQDHTVNHPDLEQTSPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAA
                     RLNYKLGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            complement(14773..16008)
                     /locus_tag="VH752_001202"
     CDS             complement(14773..16008)
                     /locus_tag="VH752_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ8289560.1"
                     /translation="MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSEPHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDHRYPGSTLEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKN
                     QNTRFEQANRLLDYVARTYCCRKILKKGSLVSERSLPIQDGQVKELPVSVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(16166..17353)
                     /locus_tag="VH752_001203"
                     /pseudo
     CDS             complement(16166..17353)
                     /locus_tag="VH752_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943304.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(17356..17576)
                     /locus_tag="VH752_001204"
                     /pseudo
     CDS             complement(17356..17576)
                     /locus_tag="VH752_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
     gene            17663..17806
                     /locus_tag="VH752_001205"
     CDS             17663..17806
                     /locus_tag="VH752_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289561.1"
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHG
                     VSH"
     gene            complement(17877..18095)
                     /locus_tag="VH752_001206"
     CDS             complement(17877..18095)
                     /locus_tag="VH752_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8289562.1"
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADV
                     LVQFSKLYNVSIDYLLGQTDYPYRFHNK"
BASE COUNT         5678 a         3916 c         3096 g         5442 t
ORIGIN      
        1 atatattcta tgatttaagg actaccaacc tgacgctttt agcgacaatc tcagcgactc
       61 cttctttaaa ggcatctgga ataatattag tagtgctcaa agcgtcatct gggacaagac
      121 tggcaatacc ttttgcggca gcaatttgca tctcaacagt aatggttttt gcccgtgcat
      181 ctaatgctcc cctaaaaata cctggaaagg caaggacatt attaatctgg ttaggaaaat
      241 cactgcggcc agtacctaca atgtaagccc ccgcttcaag ggcttcatca gggtaaatct
      301 ctggaatagg gttggccatg gcaaaaatta ctgggcgcgc tgccatttta ctaatccact
      361 ctgcttttaa gactcctggt gctgatacgc caatgaagat atctgctcct tcaagagcat
      421 cttctaaggt acctgattta aactcacgat tagtgacttt cgctatatcc aaatgatgtg
      481 gcgctaattg agcggcttct tgttcattga taatgccaaa tttatccact actgtcactt
      541 tcgtagcacc tgctgctaat aatttacggg taatggataa tccagcagat ccaccaccgt
      601 tcaccacaat ggatacctca tcaagggatt ttttaagaag tttcaaacta ttgaagatgg
      661 ctgctaacac cacaatagct gttccatgct gatcgtcatg gaaaacagga atatgacatt
      721 ccttaatcaa gcgctgctca atttcaaaac agcggggcgc actaatatcc tccaaattaa
      781 taccaccaaa ggttggtgca agcgccttga caatactaat aatttcttca gtatctttgg
      841 tatctaatac aatcggaata gcatcgacac ccgcaaatgc tttaaaaagg gctgcttttc
      901 cttccataac tggcatggct gcttcagggc caatatcccc cagaccaaga accgcagtac
      961 catcactgat aacggctact gtatttttct tggtggttaa gtcatatgct aatgttttat
     1021 cttttgcgat ggcagatgac acactagcta cacctggtgt ataggcaatg ctcaaatcat
     1081 gtttattttt aatgtcaact tttggttgaa cttccaattt ccctccgaac gttttagctt
     1141 gttcaagggc aagttgacct aattgatttt ccatagggaa tacctctctt ttttattggt
     1201 ttggattaac taaaaataat tcttaaaata gctgtcatag tgataacggt aatagcccca
     1261 cctaaacggg tagcgacttg ggcaaacggc attagattca tgcggtctgc ggtactcaaa
     1321 atagccacat cacctgttcc acccatacca ctttgacaag ctgaaataat agctgcttcc
     1381 actgggttca tgttaaggaa acgagagaca aaaaaaccga cactgacaac cgttaacacc
     1441 acactaatca ccacaataaa atattgccag ctaagggtgg ccacaacatc ttttaatggg
     1501 atataaagga gtccaagccc tgccattagt ggaaaagtaa agttgctgga aataaattta
     1561 taaagttgtt tggcaccatt ttgtgtttct tgggggataa cattcaggta tttcaagaag
     1621 gccgccaaga taatcatcaa caccggtcct ggaaaacctg ttaaatgctg taacaagcca
     1681 ccggcaataa aaagtgaaca agctgttaaa actcctgccc ccattaattt aacatctaag
     1741 gctccagaat tgtcttttag ggcatcagac atattttcgc tgtgaccaat tttaaccagt
     1801 tgcccttgac cagaataact tggatgcttc tccccaaaac gattgagcaa agccgtgcac
     1861 aagatagcaa agaaattccc aataatggtg gctggaatca gttgagccac taattgctca
     1921 cttccgactc ctgtaatggc actgtaacca agagataagg gtaaaatacc ttcaccaatt
     1981 cctccagcaa ggactggcgt cacaacataa aagagggtat gttgccagtc taatcctaaa
     2041 atgacgccaa ccaaagtccc tacacccatt gcacaaacca ttccaagtaa catgggaata
     2101 atcatccgaa aaagaccttg aatcaagatt tttcgattca tgcctaaaat actaccgcag
     2161 accaggcagg caatataaaa ataaagaaaa ttggcttgct tcatcaagac attcgtcgct
     2221 tctaagacat tgggattgag gagattaaaa aaaaccaaaa tcgaagggac taagagtgat
     2281 aaaatagctg ggccaccaaa atgtttgagg cctggaatgg tcgctccaat ggttcctagt
     2341 agccaaccaa gggttaaaat aacagcaaag ccacccagca tattaacagg caattgttgg
     2401 agccagcctg tgactaaaat taaggtagct aaaaccagat aaacaggcaa gggaacggag
     2461 cctattctcc tattttgcca tgcttttgaa tgctcagaca agtctttttg aggcattttc
     2521 ttgcttattg tttccatgaa acaacctcct ttagtgataa ggctagtata aaagagaaca
     2581 tgtcaaaagc atagtttatt taagttgttt aactacttta attttttcaa gttatttaat
     2641 taatttaagc tagcaggtca tttcttatcg tgttatactc aataaagagg agaccttatg
     2701 aaaaaaccac tacgtttatg ggccagttta tcgttgattt tggtatctat gattgttgtc
     2761 acaacctctc tattttacgg gattatgcta catgatacgc accaatccat caaaaatcag
     2821 gaaacccatt tgctaacgtc aactggaaaa atgttagcaa gtcatcaagc gattaaagaa
     2881 ttactcctaa acaaccaacc aaatgctaaa acaacagcct ataccaactc cattgcctcc
     2941 atctataacc ttgattatgt ggtcgtgatg aacatgaaag ggattcgact cacccatcct
     3001 aacccgaaaa atatcggaaa accttttcaa ggaggtgatg aggaggctgt tctagcagga
     3061 aaaaaggtta tctctaccgc caagggcact ttaggaaagt cacttcgcta tctagtacct
     3121 gtgtttgacg gtgacaaaca gattggtgct attgcggttg gcattaaact aactacccta
     3181 aatgatgtgg ctttaacgtc aaaacgcaat tataccctgt ctttactcct ttgtcttctt
     3241 attagtttac tggtcaccag ttttatttct tttcgactca agcgtcaact gcaccagtta
     3301 gaaccaagtg aaatttatca gctctttgaa gagcgtaatg ccatgttgga tcaaatcgaa
     3361 gccgccgtat ttgtggtaga taaagctggt atcttacagc tctgcaatca ggctggacaa
     3421 aaattaattg ctcggaaatg ccagttggga aaacctactg gcaacagttt caatcacctc
     3481 tttcctgatt ttccaaaact tagtttgcaa gaggggcatg aacaactctt tcggtatggt
     3541 gaggaagact acttgttagc catttcaccc atctgtgtca aaaacgatca ccgaggccac
     3601 attattttca tgcgagaagc agttaaggct attgatacct tggatcagct agcctatacc
     3661 accgcctatg cttctgctct acaagcccaa actcataaat tcatgaatca attgcatgtt
     3721 atttatggct tggtggatat tgcttattat gaccaattga aaatttattt agacagtatt
     3781 ttggagcccg aaaatgaaat tctcactagc ttatctgtcc ttgttaagga gcctcttcta
     3841 gctagttttc tcattggaga gcaagagaaa taccaagaat taaatgtcca tctcaagatt
     3901 gatgttttaa gcgagattcc tcattctgcc accaagaatc aacttaataa tggtttgatg
     3961 atttaccgct ttatccatac taatcttcta accacactga gacccaaatc actcgttttg
     4021 agtattcaac atgatcagaa tcatttaatc agccattaca ccttgacaga caattggatt
     4081 gatttggagc gggtacagcc tatttttgac ttaccttatt ttcaacagct cttgaccgat
     4141 acaaattccc aattccatca acatcaaacc gtaaccgagt tacagttctc agtgacaaca
     4201 ccttatgtag gagggcaaca tgaacgtttt aatcattgaa gatgatccta tggtggattt
     4261 tatccatcgc aattacctcg aaaaactaaa tctttttgat cgcattatat ccagcgactc
     4321 tatgaaagct gttcaaagca tacttactga ttacgcaatt gacctcattt tactggatat
     4381 tcatattaca gatggcaatg ggattcaatt tttggagaaa tggcgtgctc agcatattcc
     4441 ttgcgaagtc attattattt cagccgcaaa tgatgggaat attatccgag atggttttca
     4501 tttaggaatt atcgactacc tcatcaagcc ctttaccttt gaacgctttc aagaaagcat
     4561 ccaacagttt gttacacatc gagaacattt ggccaatcag caactagagc aggcccaaat
     4621 tgaccaactg aaatgtttga ctagcaaaaa agacactaaa aacaaacaat tgttagaaaa
     4681 aggcttatct gaatccactt tccagtggat tatggaaaat atcaaagcct ttgatcaacc
     4741 ttttactatc caagaattag cttccgcttg tcacttatcc catgtctctg tccgtaaata
     4801 cattgcttat ttagaagaaa ataagcaact gaacagccaa caaattttta ccaaggttgg
     4861 ccgtccctat cgcgtatact ggtgacgtca tctgtcgata cattaaaaag gctggagaaa
     4921 taccagcctt tttgagtgct tatttgcgat acattttaaa ttggaggtat aaatcattat
     4981 aaatccccaa ccatcttgac cctaaattgt cataaacttc caattttttg ataatgtcat
     5041 ctgttggata aaaagcagga tcatttttta tctcatctgg aagtaaggcc ttggcttttt
     5101 tatttggtgt cgcataacca atatatgcag cattttgcgc agcattttca ggacgattga
     5161 taaagttcaa aaaagcataa gcttcttttt cgtgtttcat ggtttttggt agtaccaaat
     5221 tatcaaacca aaggttagac ccttctgaag gcacgatgta gtgaaggtgt tcgttactat
     5281 ctaacatctc actggcttca ccagaaaagg taattccaat agcagcgtca ccttgaatca
     5341 tgtagccttt catctcatct gctacaatgg ctttaacatt cggcgtcaac tgctgcagtt
     5401 ttctctcggc tgcctgcaac tgctccagat ttttagaatt cacactataa ccaaaagttg
     5461 ttaaaccaac ccctagcatt tcacgcgctc catcaatcag cataatacta tttttatatt
     5521 ctggacgcca cagatcttcc cagtgcatag gcgccttatc aactaattga tcattataaa
     5581 caatcccaac ggttccccag aaataaggca aagaatagtc gttttgtggg tcaaagcttt
     5641 tccctaaaaa ttctttcccg atattatcca tgccaactaa ttttgactta tcaagcttat
     5701 tgagtaggtt ttctttgatc attttatcaa tggtgtaatc actaggaaca gcaatgtcgt
     5761 aagtggttcc gccctgcttg attttagtgt acatggcttc attggaatcg aaagtttcat
     5821 actgcacttc aatgcccgtt tctttggtga attttttgag caaagctgga tcaatgtaat
     5881 ctccccagtt ataaataact aatttatccg attgactacc agaacccgat tttttctgca
     5941 agatgaaaga aagacttgtt aaaataacaa taacacccaa aactcctgct agaaaggaat
     6001 aaagtttacg catgtttttc ctccttgtcc tgtgacatat aataataacc gatcactaag
     6061 aggatggaga aaaagaaaac gatggttgac aaagcattga tatccaagga aattccctga
     6121 cgagcccgcg aataaatctc aacagataaa gtagtaaaac catttccagt caaaaagaaa
     6181 gtcactgcaa aatcatctaa ggaataggta aaggccataa aataacctgc aataatccct
     6241 ggtgttaagt atggcagcat gacttctttg agcatttgga aataattagc tcccaaatca
     6301 taagcggcgt tgaccatgtc ttgattcatc tctttcaagc gcggcaatac catcaaaacc
     6361 acaataggaa tcgaaaaagc aatatgactt aataaaactg aagacatgcc cagctgaaac
     6421 ttcaatgatg taaaaagaat taaaaaggaa gccccaatca tgacatctgg tgataccatc
     6481 aagacattat tggctgatag catggcattt tggtacttac ctctaacatg gtggataaag
     6541 atagctccaa aggtcccaat aatcgttgct agtagagcgc tactaaaagc aagaacaaag
     6601 gtttgcagta agattgtcat gagacgacta tcctcaaaca tggtttggta atgctcaagg
     6661 gtaaatcctg taaaaccatt catatcccca cctttgttga aagaatagaa aatcaaataa
     6721 aaaataggaa tgtagagtaa aacaaagaca ctcgctaaat aaagattggc aaattttttc
     6781 atttactttt ctcctttgtg agccacataa tagcaaccat ggtcaaaatc aagacgacac
     6841 caatggttga tcccattccc cagttttggg tggtcaaaaa atgttgttca atggctgtac
     6901 caagtgtaat cacgcggttt ccaccaatca aacgggttaa cataaagaga ctcaaacttg
     6961 ggataaagac agactgaaca cctgccctaa ccccatttaa agaaagggga aaaataactt
     7021 ttgagaaggc ctgaaattca ctagctccta gatcgcgact ggcattgatg acattatggt
     7081 caatatcatc caaagcatta aaaatcggta acatcataaa aggaagctca atgtaagagg
     7141 ctacaaaaat gaaggagaaa tccgtgaaaa ggatttgctg cgggccaatc cccataaagg
     7201 ttaaaaagct gttaatccct ccttgttgac caaagattcc cataaaggca taggctttta
     7261 gcaataagtt tacccaagtt ggcaaaataa tgagcataag ccacaactgc ttgtgcttta
     7321 ggcgcgttaa aaagagagcc gtaggatatg aaattaagag cgtgacgagt gtgataatac
     7381 cagcgtataa aatagaattc acactcattc tcaaataggt ccaagagcta aaaaaggttt
     7441 cataattggc taaggtcacg cgcccttcta tgtcaaaaaa ggacttgtaa aacaagagag
     7501 tgactggtgc cacaacaaaa aagagaatcc ataagaagta aggaatcgaa aaaagactag
     7561 aggttttctt cattacgctc ctcctcaatg gcattaatca atccatcttc aggctcatcc
     7621 atttccacat attcttcaat acgggcatca aattcctctt cagtctcatt aagacgcatg
     7681 atatgaagat cttcaggggt aaagtctaat ccgataactt ctccctcgat agctttgcgg
     7741 gtagaatgaa tcatccattc attacccaat tcatcatagg caataatttc gtagtgaacc
     7801 ccgcggaata attgggtatc aaccttaact tgtaatttcc cttcttctgg caaagtaatt
     7861 tgaagatctt caggacgaat aaccacttca acaggctcat tagggcgcat tccaccgtcc
     7921 acagattcaa attctttccc gttaaaggag acaagatagt cttcaatcat ggtaccgtta
     7981 ataatattag attctccaat aaaattagca acaaaatgat taattggctc atcgtaaata
     8041 tccactggtg ttcccgactg aacaatttca ccttcattca tgacaaaaat ccaatcactc
     8101 atggccaagg cttcttcttg atcgtgagta acaaaaacaa aggtaatgcc tagacgttgt
     8161 tgcaattcac gtaattcata ttgcatctct gttcttaatt tcaaatcaag ggctgagagt
     8221 ggttcgtcaa gtaagaccac acgcggttga ttgataatcg cacgcgcaat ggcaacacgt
     8281 tgacgttgac caccagataa cttctggata gaacgatttt catacccttc taattgaacc
     8341 attttcaagg tttctttcac gcgcttagca atctctttct tgtccacttt cttcagcttc
     8401 aaagcaaagg caacattctc aaaaacagtc atatgaggga agagggcata attttggaaa
     8461 acggtatgaa tgtcacgttt attaatcggc aaatcattaa tacgttcccc atctaaataa
     8521 atgtctccgc tgctggcatc tagtaggccc gccataatat ttaaaatagt tgattttcca
     8581 gagccagaag ctccaagcag ggtgtaaaat tttccttctt caaggtcaaa gttaatgttc
     8641 tttagaacct gtgttccact gtcctcaaat gtttttgaaa cattgttaaa tgtgataatt
     8701 ggctcagtca attgtcataa atcctccacg gtactttctt taattatcaa taatggttac
     8761 aggttttccc caataatccg aacttctggt tcaagcttga caccagaatg gttttcaact
     8821 gtctcaatga cataagcaat caaatcttca taatctttag ctgtgccatc tgccacatta
     8881 atcataaaac cagcgtgttt ttcggaaact tccacaccac caatccgatg ccctttaaga
     8941 tttgcttcca tgattaattg acctgcaaag tgtcctggcg ggcgcttaaa cacagatcca
     9001 caagatggaa attctaaagg ttgttttagt tggcgaaggt gatttaacct attcatttct
     9061 tggctgatcg tatcataatt acccggctta agagcaaact tagcagaaat gacaatgtca
     9121 cctgtttctt gaatggcaga gtgacggtaa ccaaaagcca tatccctagc tgaaatggtt
     9181 ttgatctctc cacttggggt aagcacctta gcggataaga aaatatgtga tatttcccca
     9241 ccataagcac ccgcattcat aaaaacagca cccccaatgc ttccaggaat gccacatgca
     9301 aattcaaacc ctgttaaact atggaatttg gcaatcttcg ttgtttcaat cagattggca
     9361 ccagcttcgg cttttaaggt ataaccattt aagtgcactg catttagctt atcaaacatg
     9421 atgacaaagc cacgaatacc accatctcgc acaatgagat tgctggcatt tcccaaaaca
     9481 agccaaggca tattttcttt gttggcataa gcaacgatgc gtgatagctc gtagtgattt
     9541 ctaggaaaag ccaagaaatc tgctgggcca cctaccttag tgtaagtgta atgttttagg
     9601 ggttcattct ctcgaatatc aatcccatga agttcagtta tcatagttat cttctttctt
     9661 ttatcacttc ttatacgcgt aaaaaaatcg gcataaagcc gatactatta tatcaaattt
     9721 tagaaaaaat aacgatgaat ttggcaaaac tttatggatt ggcttatagc aggcattact
     9781 ccgaaaacag agtctagaac tctgtctgtt gagctgaaaa gagcgtgata tctgattgat
     9841 ctaaagctgc aagataatcc cttaaaaact tatgattagg aggcaatttt aaatctggct
     9901 gcaattccaa taaggggatt aaaacaaaag cacgctcagt catataaggg tgtggcactt
     9961 tcaattcctt tgtgtcataa acctcttctc caaatagcaa gatatcaatg tcaatagttc
    10021 ttgatcccca tttttcatgc ctcacacgac caagagattg ctcaatagct tgtgtttctt
    10081 ttaagaaatc agccgcggtt aattgtgtat ctaattgaca ggccatattg agaaaatcag
    10141 cctggcctgt cttgccccaa gcagtcgttt cataaatgga tgattgagca agcaaccgtg
    10201 tttgaggtaa gtcagctaaa gcctcaagag ctttttgcaa gtaagctgct cggtccccca
    10261 tattggtgcc taaacttaaa taaacaatgg tcatctctgt ctctccaatt caataccaac
    10321 tgccttatag tggcctgcaa tcggtgggtt ttccttttta atggccactt tgatggcttc
    10381 aataggtgga aattcgttaa aaagctgttc acaaatcgca cctgctaaac gttcaatcaa
    10441 aataaacttt tccccttcca ctaactgacg gacactatca aacaccatcc cgtaatggac
    10501 cgtatctgtt aattggtctg acaaagaagc tgcttgcaaa tcgactgaca attctaaatc
    10561 aaccagaaaa atttgcccaa gggtctgttc ttctttaaag gctccatggt agccataaaa
    10621 acggcaacct tctaatacga ttttatccat aagtcctcac atcaactggc tcaacacagc
    10681 cacaatgtct tgattagcct tgacatcgtg tacgcgtaca atctgacagc cttttccaag
    10741 ggcataagca gacaaggctg ctgtcgctcc gtctcgctct ttagctttgg tgttgccgcc
    10801 taacaaggca tctacgacac gctttctcga aataccaaac aagacaggat agcccaaatg
    10861 acagacgcgg tccaatcctt ttaataactc cgtattctgt tgaacagatt tggcaaatcc
    10921 aaatcctgga tcaacccaaa tattgttttt tggcacgcca gcatcaagag ctgcttgtgc
    10981 tctattgcct agaaaatcac aaacgtcttg tgttacctct tgataaactt cttcgtcttg
    11041 gttatgcatc aagatgatag gcgcatcgta ctcggctgct aaggcaaaca tttgaccgtc
    11101 gtacaaacct gcccaaacat cattgagaat atcggcaccc gcctctaaag ctgctctagc
    11161 tgtttcggtt ttataggtat caatgctgat tagaatatca tagttttctt tgatggcctt
    11221 gatcacagga accacccggt caatttcatc ggtagcgctt acaaattggc aacctggacg
    11281 tgttgattct ccaccaacgt cgatgatttt agccccatca gcaatcattt gctcaacgtg
    11341 atctaatgct ttttgcacag tagtgtaaga ccccccatct gaaaaagaat ctggagtcac
    11401 atttaaaatc cccatgatag ccgcattgcc ctcaatcaca aactttccaa ttttcataag
    11461 ttctcctaat cttttgtcat tagagaaata acttcttgcc tctcagcacg actttcctta
    11521 tataagcctc tggcagtggt ggtaatggtt ttactacctg gctttttgat gcctcgcatg
    11581 gtcatgcaca tgtgctctgc ttctaccata actaaagtcc ctttaggatt gagggcttca
    11641 actaaagcat ccgctatttg agaggtcaaa cgctcttgga gttgaggtcg tttactagct
    11701 acttcaactg ctcgcgctaa tttactcaaa cctgttactc gaccatcact tggcaagtaa
    11761 gcgatatgag ccttgccata aaaaggaact aagtggtgtt cacatataga ataaaaacta
    11821 atgtctttga caatcaccac atcctcatgt tgttctttaa agacagccgt aaactcctct
    11881 ttaggatctt ttcccagtcc caaaaacatc tccgcataca ttttagcaac ccgtttaggc
    11941 gtgtcaagaa gaccttcccg atttggattt tcaccaatag cctctaaaaa ctgataaatg
    12001 gctgcttctg ctttttcctt attaatagac attagtcttt cccttttcat tagttttttc
    12061 cttttccttt tcattttctc ttttttaatg ggttaaaaga acgttttttt caatatgttt
    12121 tttccaatac tgtctaattt ctgaaataaa atatagcgac cctgtaatca cgaaaaagtc
    12181 atctgctttg gcatgcttac gcagggccaa gaaatctttg aaatcagcaa ccctaccaaa
    12241 acgttctggg tatttttcca atggataggc gttagggtaa tgaaagccag taacctgcaa
    12301 gtcacctatt tgctcaagag caaccaacat atccgcaata ggcttggtgt cgatcgcacc
    12361 aaacaaaata tgaagctttt tatcattgta gttatttttt aagacggcta ccaaagcagc
    12421 aacgctttca ttgttatgag ccccatcaat cattagattt ggtgctaata attcggtacg
    12481 gcctagccac aaactatttg ccagaccttc ccttatatga tctgaagtta gcctaggata
    12541 gcggtctttc aaggttaagc aagtcataat agctaaagct gcattagaca cttggtggtg
    12601 tcctggcata gcgatgtgaa tatctgaaat aactcctaaa ggactagtaa aacgatagcc
    12661 agatgcgttc tcagccattt gaaactgctc ttgccattcc cagattgaag caccaacctg
    12721 ctcagctttt gttaaaaaaa cctctcgagc tgacgggttt tcaacggcaa aaacaagggt
    12781 ctcgccccct tctaaaacac cagctttttg ggcggcaata tcagcgtaag tctctcccaa
    12841 aatagcttgg tgatctaaac caattgaggg acataccaca accattgctt gaaaaacatt
    12901 ggtcgaatca taaagaccgc caaggcctgc ttcaataata gcaatatcta caggatgcat
    12961 gtctccaaaa tagaggaaca tgatcaaagt aatcacttca aattcagtta cctctccaaa
    13021 atcagtctct tgaactagac gctctgtcaa aggacgaata cgattagcag caataaccaa
    13081 atctttttct gaaatcattc gaccatttat gctaatacgt tctttgaaat ccataatata
    13141 aggagaggta aaggtcccca cttcataacc agctgtcgta aaaatgtgtt gtaaatgatt
    13201 gaccgtggaa ccttttccgt tagttccaac gatatgcact cccttgacat ttttttgggg
    13261 atttcctaac tgtccaagaa cccaaagcat acgttttaag cctggcttga tgccgaaaac
    13321 taagtgatca tgaatccatt ctagagtctc ttcataagtc atttcacacc tcacatctat
    13381 ctttagtata ccatatataa aaaaaagtga cagcagataa cctagagtag tacccttttt
    13441 ttattatcct caccaaacac tcaaataagt tctaaagtac actacacttt tatcttcatt
    13501 gcttttttta cgtgataaga ataccagccc cacatagtct ttgctacctc ctaaagtgtt
    13561 atctgtgaca ataatttttt tactccttaa tacacgttct tccaaaaaac aagaaaaaag
    13621 gctgacttca agccttcttt tctctaaaaa aatgttacaa tagaggtact tatatcaaaa
    13681 aaaggagcaa gtaatgaaca atagacataa acggcgccaa aaaagaaaag ccttcactct
    13741 cattaacctt atactattat ttgcctgcat tcttggtttg accctcattt tctatttata
    13801 ccaagggtct aacaaattcg gcttgtcaga tgtctctcaa tttgtaccta aaataagtct
    13861 ttttcatcac aaaaagacgg caaaaaaaga aacgacaaaa cttaagaaga ctcatttcga
    13921 ttcctcaaaa tctcaaaaaa aggcccattc taaattaact tggaccaagc aagaaacacc
    13981 tgtcaagatc cccattttaa tgtatcatgc tattcatgta atgtcccctg aagagacggc
    14041 caatgctaat ttgattgtca acccagatct ctttgatcaa caacttcaaa aaatgaaaga
    14101 cgagggctac tattttttga gtcctgaaga ggtctaccgc gctctttcaa ataatgaatt
    14161 gcctgctaaa aaagttgtat ggctaacatt tgatgacagt atgattgatt tctacaacgt
    14221 tgcttaccca atcttaaaaa aatatgatgc taaagccacc aataatgtta ttacaggact
    14281 aaccgaaata ggaagcgctg ccaatctgac tcttaagcag atgaaagaaa tgaaacaatt
    14341 cggtatgtct ttccaagatc atacagtgaa tcatcctgat ttagagcaga ctagtcctga
    14401 tgttcaaaca actgaaatga aagattcaaa ggactatctt gataagcaat taaaccaaaa
    14461 cactattgcc attgcttatc catctggtag atataatgac actactttac aaattgcagc
    14521 tagacttaac tataaattag gtgttaccac taacgaagga atagctagcg cggctaatgg
    14581 acttctttct cttaaccgga ttcgtatcct cccaaacatg agtccggaaa atcttttaca
    14641 aacaatggaa ccataacatc aattttttta tcattccaaa tcatactcac tgtaaactaa
    14701 aaagacagtt atgaaaaaat tctttcataa ctgtcttttt ttgattgttt taaaattatt
    14761 ttattcttat ctttaacgtc ttttttcaag aaaatctcgc caccacagct gtaagtcaga
    14821 ggctttttta agagattggc tagctttaag aggaatacgc ttaggttcct ttaaatacct
    14881 cactgactga tctgttattc ttggagacgt caaataagca accacttcac ctttagtaat
    14941 cggtgctagt aaagaagtct ctgatatcat aaattgttta ggtttgggaa cttgttcacc
    15001 ctgttgcaaa atgatggtta catcttcggc aacagatacc ggtagttctt ttacttggcc
    15061 atcttgaata gggagcgacc gctccgagac aagacttcct tttttaagaa tcttacgaca
    15121 acaataagtt ctagccacat agtccaacaa acgatttgct tgctcaaacc gcgtgttttg
    15181 gtttttatca gatccatcgg catttagaat aacggtaatc actcgcatac cattttcaat
    15241 agaagttacc acaagacaat gacctgccaa gtctgttgtc ccagtcttta aaccatccac
    15301 tcctgctcta cccttagcca tacctggtaa gagttgatta aaacttttaa tagaattgcc
    15361 agcaaaatta acttctgttt gtttggtgat ctctaaaatc tcaggaaaat cttctaacaa
    15421 atgcatcgtc acaactgcaa catcttgagc actcaaggta ttttcttctt ccaacgtaga
    15481 accaggataa cgatggtcct ttaacacctc atttggcaga cctgaggcgt tgaggatttt
    15541 accagaagta atcccccaat gagatagctg ttctctcatt tgatttacaa aatgaggctc
    15601 tgagcccgcc actttttcgg ccaacgcaat agcagcgcta tttgagcttg ctaccaaagt
    15661 agctgtcaac aggtcttgaa ctgaataagt ttttttgtca aaaggtacat tacttaaaga
    15721 cctatctttt gttaactcga aggcataatc tgagagatta acttgatctg ataattgcag
    15781 ctggcctgat ttaaccttat ctaaaaccag ataagccgtc accaatttgg tcaaagaggc
    15841 aatagcatct ggcgtcttag cgtctttctc atacagaact ctcccagagt ctgcctctac
    15901 gacaatcgca tgttctgctc ccacttgaaa atcagctgcg agaactgttt taccaaaccc
    15961 tgaacaagtt aataccataa aaaaaaggat aacccacctt tttatcatta tatctttcat
    16021 agttactatt atagcaaaaa ttctcagaaa tttggaagga agtatagtgg cacacacctt
    16081 tagtagctta aacaagctca taaagcaagt accctgtaac aataacacat cctgttatat
    16141 gggcgatata ataagcttcg ttatatcaat tagttgtttg gcaggaattt tggtataaaa
    16201 tggaatctaa tcctgaactt attacatttt gggttatttc ttcagatact gttttatcta
    16261 atgcgttaaa tcagtgacta tatgtattca aagttgtaga cttatcagca tgtcccatac
    16321 actttgcaac gtacagaatg tcttttttca aaacattaat aagataggaa gcatgactat
    16381 gtcgcaagcc ttttcccgtt atcactggta ctcctacttc ttgagctttg tgcttaataa
    16441 ctcgacaaat cgttgattta caaaatggtt caccaaaccg tgaaataatg aaatctgtgt
    16501 cagtattgac tacttgattt cttctccaat tttccaatac tactattgtc acatcatcta
    16561 actcaataag acgttctcct gctggcgttt ttgtttggtc tttacgatac caatttccat
    16621 tttcatcctt ttccaaggtt gtatgaactt tcaagaattt ttgttcgaaa tcgatatttt
    16681 cccatcataa tgataaacct tcacttacac gaacacctgt catataatat agccaaatag
    16741 ttgtaaatct ttgtagctct tcataatctt gcaaatcaaa cgattttata aaattctgaa
    16801 actcaatata agtccaaaat ggagtgtcgg gatgtttccc tttaggatta tccaaagcct
    16861 tacatggcac ttttgaaata tagcctaacc tttcagcata ccccatgcaa gctttgaatc
    16921 gcgaccataa attctttgca taattttcaa taatatgtaa actaaaagat tcacaatctc
    16981 aagctgttat tccctttaaa tgtttagaac caaaatactg aataaataac ttatgatgtg
    17041 gtaaggctat ttcataagtt acagcctgaa ccttttgctt atatgctctt aggtaaatag
    17101 tattcatata gttttcaaac gatatattgt cattttcaat actaagatta ttactgaact
    17161 catacctaat ccgaaccagt tcatcatacg cttctttcat tgtaccaaat ggctttccat
    17221 actaattctt tctggctttc ttttgaactc gtttacctgt tactggatct acaccaagat
    17281 cagcttgata aaatatcttc ccattcttat cacaataaac acctttgtac tttttttgat
    17341 tttttatagt catctttaat tctcactttc taaactttct tcagagagta gaatacctat
    17401 taactcttct acaatatctt taggagcaat accaagtctt ctattgtcaa agggcgattt
    17461 acttaattgt accgcatttt gttcaagttc gcgagcttct tcaacttttt tatagctatt
    17521 ctttttgctt ctctaataat atttcttgca gtatgttcga gaaaaccaat attcatcaag
    17581 ctttttatat gttactattt tcacacctcc actcctttca aaaaaattac aacctccgtg
    17641 ggtatgactg gttaaatcac tcatgggaat ctcacccact ggccttttac gctggcagcc
    17701 tgaacgttca gaagtatcat tgtccctatt tgtggtcatg gcagatagag aaccactcta
    17761 ttttatagct aataattatc gctcctatca cggcgtatct cactaaattg ctcgacaaac
    17821 aagaaagtcc cgcctcggtt tattcagttg tcaatgaaca aatcaaggcg agacttttat
    17881 ttattatgaa aacgatacgg ataatctgtt tgccctaata aataatcaat actgacgtta
    17941 tagagtttag aaaactgcac aagaacatct gccattaagg caacctctcc tctctcaatt
    18001 tttgcataat ttgcatgaga gaaggaaagt aaatttgcaa caaacttttg agtgaaatcg
    18061 ttatcttctc tcaaattcct aatacgcgga tacatctaac attctccgaa tatcagtata
    18121 aaggagattt ga
//
LOCUS       DAUGHY010000034        17045 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.034, whole genome shotgun sequence.
ACCESSION   DAUGHY010000034 DAUGHY010000000
VERSION     DAUGHY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17045
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.034"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            266..706
                     /locus_tag="VH752_001207"
     CDS             266..706
                     /locus_tag="VH752_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289563.1"
                     /translation="MTKKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKDSAKKIDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEEKWQDTKDAVEVYGGIAAE
                     TVEESLGQAKEKVEGIGEATSQTIQSKMEKLTSDKTDADDDDEK"
     gene            complement(756..1667)
                     /locus_tag="VH752_001208"
     CDS             complement(756..1667)
                     /locus_tag="VH752_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ8289564.1"
                     /translation="MKTVRIFYNPNSGKKEDQLAGQVKDYFCQHGFSEDSVEVITPKD
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAHCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIKQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQADDG
                     YFQVYTMKKVSFFKFLWHINDFKQGDFSKAEEISHFQANTLSLLPQAKKQAILPRTRI
                     DGDKSDYLPIQLDIIPKAISIIVPTED"
     gene            complement(1784..2434)
                     /locus_tag="VH752_001209"
     CDS             complement(1784..2434)
                     /locus_tag="VH752_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEQ8289565.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(2431..2871)
                     /locus_tag="VH752_001210"
     CDS             complement(2431..2871)
                     /locus_tag="VH752_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEQ8289566.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQADSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLLQEVTQ"
     gene            3090..3938
                     /gene="ylqF"
                     /locus_tag="VH752_001211"
     CDS             3090..3938
                     /gene="ylqF"
                     /locus_tag="VH752_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEQ8289567.1"
                     /translation="MATIQWFPGHMSKARRQVQENVKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIQRLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTHY
                     PSRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYTLFVKEVRDGKLGQYTLDQV
                     GDMDVD"
     gene            3928..4719
                     /locus_tag="VH752_001212"
     CDS             3928..4719
                     /locus_tag="VH752_001212"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEQ8289568.1"
                     /translation="MSTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKA
                     IQAELAEEERLEAMLSYEKALYKKGYQAIAGIDEVGRGPLAGPVVAACVILPKYCKIK
                     GLNDSKKIPKSKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     SQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            4784..5620
                     /gene="dprA"
                     /locus_tag="VH752_001213"
     CDS             4784..5620
                     /gene="dprA"
                     /locus_tag="VH752_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEQ8289569.1"
                     /translation="MNHFELYKLKKAGLTNKNILNILDYQEHQEKSLSLRDMAVVSGC
                     KHPSHFIEAYKQLDIQNLKIEFKQFPSISILDKHYPMALKEIYNPPVLLFFQGNLDLL
                     EKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIVSGLARGIDTSAHLACLKNGGQTI
                     AIIGTGLDRFYPKENRELQTFLGKNHLVLTEYGPGEEALSYHFPERNRIIAGLSRGIL
                     VVEAKNRSGSLITCQIGIEEGRDIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILS
                     EY"
     gene            5727..7856
                     /gene="topA"
                     /locus_tag="VH752_001214"
     CDS             5727..7856
                     /gene="topA"
                     /locus_tag="VH752_001214"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEQ8289570.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSIAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSINFDNNYEPQYINIRGKGPLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDG
                     LFKKGTKKFQATFYGINGKKTKLDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLP
                     YTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQ
                     NDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKL
                     YTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKMLP
                     EMEEGENVKKVSTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEVIQRRYY
                     VKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEEWQHVIDQF
                     YQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNT
                     KAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDY
                     LIEKKVRGGKQVMCSNETCDYKEEKIK"
     gene            complement(7931..8815)
                     /locus_tag="VH752_001215"
                     /pseudo
     CDS             complement(7931..8815)
                     /locus_tag="VH752_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8828..9394
                     /locus_tag="VH752_001216"
                     /pseudo
     CDS             8828..9394
                     /locus_tag="VH752_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
     gene            9409..10401
                     /locus_tag="VH752_001217"
     CDS             9409..10401
                     /locus_tag="VH752_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEQ8289571.1"
                     /translation="MKLKLYNVRGEEAVLAKKWADDNGIEISLTESSLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIALNLIRHVELIRENVKKQNFTWRLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGCKVVGYDIYQSDAAKAVLDYKESVEEAIKDADLVSLHMPPTAENTHLFNA
                     DLFKSFKKGAILMNMARGALIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYYTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
     gene            10506..11171
                     /locus_tag="VH752_001218"
     CDS             10506..11171
                     /locus_tag="VH752_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEQ8289572.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLALRTGHHNQDDVLNSLLAAGDAIKANWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     TDD"
     gene            11164..11880
                     /locus_tag="VH752_001219"
     CDS             11164..11880
                     /locus_tag="VH752_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEQ8289573.1"
                     /translation="MINGVSHYLAQTPTLTLFLICHVLSDYQLQSQQVADLKEKHLTY
                     LGYHLIGVSIPLICLTLIIPQAWLMSLLVMISHALIDWLKPKMANSLKWKREWIFLLD
                     QCLHIAISSFAGLRLAGVTLPNWLPISILMTVLFILVITKPTNIVFKLFFSKYQPDQG
                     EKMDTIIGAGATIGILERIVIGLCMIMGQFASIGLVFTAKSIARYNKISESPAFAEYY
                     LIGSLFSILSVFIAAWICFF"
     gene            12043..13389
                     /gene="trmFO"
                     /locus_tag="VH752_001220"
     CDS             12043..13389
                     /gene="trmFO"
                     /locus_tag="VH752_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEQ8289574.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAESVTAELENHPLIDVIRDEITEIPDDAITVIATGPLTSDALAEKIHAVNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPL
                     NSFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRNNPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKREEALVFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL"
     gene            13566..14963
                     /locus_tag="VH752_001221"
     CDS             13566..14963
                     /locus_tag="VH752_001221"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HEQ8289575.1"
                     /translation="MQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTILDKIGYYSL
                     ECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHYADDIVDKF
                     ISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVHTLNYYLSL
                     VKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSGISEMTYLK
                     VAEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRD
                     HYRETGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLAEQGLADKYEEVLAEVPKVRAD
                     LGYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKI
                     IGDEAVIACRPADLIEPQMIYLRDEIAPYARSEEDVLSYASFPQQARDFLGRREDPFY
                     DVPVQEVTVQLDIQD"
     gene            complement(15074..16126)
                     /gene="citC"
                     /locus_tag="VH752_001222"
     CDS             complement(15074..16126)
                     /gene="citC"
                     /locus_tag="VH752_001222"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEQ8289576.1"
                     /translation="MPYYTVSKVFPSDKTTMASVKNLLHQEGIRLDAHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGEGLLNRIVSHLIDEEYALGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAIVINANPFTLG
                     HQFLVEKAARENDWVHLFMVSEDQSLVPFSVRKMLIQEGLRHLDNIIFHETGPYLISQ
                     ATFPAYFQTEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDNDWDLLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(16215..16697)
                     /locus_tag="VH752_001223"
     CDS             complement(16215..16697)
                     /locus_tag="VH752_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEQ8289577.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTIDDVINLPVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
BASE COUNT         5372 a         3102 c         3401 g         5170 t
ORIGIN      
        1 ttgacaacca tttattggtc cttttagata agacaatttt gtggctcttc agttgaaagg
       61 atgtgttgtt gatgttagag gaaacaatca tgttaaatta actcaggctt aaagaccaaa
      121 aaaggagtga agtctattat gaaaaaaaga aattttaaac ttacagaaat gttacaattg
      181 gttttattag tggttcctgc tgtaaaactt gctaaaaaaa tttttaacga tagtaaaaca
      241 ccgtctaaaa gaaagtaggt ctacaatgac taaaaaaaat catggcgttt tttgggggtt
      301 gttgcttgga tcagcagcag ctagcattgc ttacttaagt ttatcgtctt ccaaaaaaga
      361 tcaactcttg aaagatagtg ctaaaaaaat agatgatctc aacgcttact tgcaagataa
      421 aagtaagcaa gttctggatg cagtatcgga aaaagtacaa gaatccaaag atgctgttga
      481 agtttatggt ggtattgccg cagaaaccgt tgaagaaaag tggcaagata ccaaagatgc
      541 tgttgaagtt tacggtggta ttgccgcaga aacagttgaa gaatctttgg gacaggctaa
      601 agaaaaggta gaaggaattg gcgaagctac tagtcaaact atccaatcta aaatggaaaa
      661 actcaccagc gataagacag atgctgatga tgatgatgaa aaataggttt taaaatacct
      721 ttaagataag acaaaaagtg ccattaggca cttttttaat cttctgttgg aacaataatc
      781 gaaatggctt ttggaatgat atctaattga atgggcagat agtcactttt atccccatca
      841 atcctggtac gtggtaatat cgcttgtttt ttagcttgtg gtagtaagct aagcgtattg
      901 gcttgaaaat gactgatttc ctctgctttg gaaaaatcac cctgtttaaa atcattaata
      961 tgccataaaa atttaaagaa agagactttt ttcatggtat aaacttggaa atagccatcg
     1021 tcagcttgag caccaggaga aaaagaagga aaaccagcaa tggtatttgt catcgtgatt
     1081 aagagaaact tcgtttttag gtgaatacga tggttgtggc taattaaaga gatggcatag
     1141 ctgcgatttc ttttcagtat tcgaatacta tcttttaaaa atgctagagg accaaagcgt
     1201 cgtttttcct cagccgttac gtcggcagca atatcagcca gtaatcctaa ggttaaactg
     1261 gaaatcatgt attgctggtt agctttgcaa atatcaactt gtttgatttg tcccgtcaag
     1321 atagtatcca acgcctcagt aatttgtaaa gggatatgca tagccttggc aaaattattg
     1381 actgtccctg agggcactaa gcctattaaa caatgagctc ctccttcata gatgccaccg
     1441 atgattttat ttagtgtccc atcgccacca agagggataa ccaagtcaat cttgtcctta
     1501 gcagcttgtt ttgctaattg aaaagcctgg tcagcatctt taggggtaat aacttctaca
     1561 gaatcttcgg aaaaaccatg ttggcaaaaa tagtctttga cttgcccagc aagttggtct
     1621 tctttctttc cagaattagg attgtaaaaa atccgaacgg ttttcatcag gtctctcctt
     1681 tgacagctta ttacttgcta tccattatac cacttctaag aattgatgtc ttacaccaac
     1741 tcatcacatt aagcctctca atcagtctga gcatctattt atcttaaagc ataaagaaag
     1801 tgatagcgat aaattggagg gcagaggcca ataaaataaa gagatgccaa atcatgtgga
     1861 agtagggtct ttttttggca taaaataccg ctccaatggt ataagacaag ccaccaaata
     1921 acatgagaag accaaaagca agactagttt tttgaatgat gactggcaag ataaacacaa
     1981 cgagccagcc cataacgata taaagcatca aactgaattt ttcattgata cgtttagcaa
     2041 aaattttgta taaaatccca aaaagcgtaa tgccccactg caaaacaata atgatatagc
     2101 ctagccaacc agaaacaagg gataaggcaa caggtgtata acttcctgcg atagcaatgt
     2161 aaatcatact gtgatcaata atgcgtagaa tgtatttgtg aaccgaacca taagccatgg
     2221 agtggtaaat cgtagaggac aaaaacatca aaaagagact gatgacaaag atggagatac
     2281 caatagcggc tttaaggtca taagtttgat aagcatagct ggctgaaata ggaagtagaa
     2341 tgagcatagc aaatgctccg atagcgtgtg tcacgctgtt tgctacttcc tcactaaaag
     2401 ataagggtaa actttgttta aaggtttggt tcattgggtc acctcctgta ataaataaat
     2461 agttttgtgg taatctttga ccgtttgaca ggcaacctgc acaatcggtg gaatgtcctc
     2521 ctcaatggga gccttctgat tccaacaggt tacaaaagta ttgtaaagac tttcagaatc
     2581 agcctgtgcc tgaagttcct ccaatacgcg actaatatct tgaataggaa ataccgtttt
     2641 aaacaaactg atggcaatta agcgcgctag ttgttgcttt tggtattttt tcttgatagg
     2701 tttagtcaca tacccatgct tgacgtaatt attaatcata gatgccgtta gtgatttatt
     2761 atctgacact tctgaaaaat cagtgcactg gttgacataa agtaagactt gatccaaata
     2821 taagtctaaa tcagggaggt ccttccaata aggtaatgat ggaaaaatca tagtttcgtc
     2881 tctctatcta gttttgataa ctagattata tgattaatga aaacttattg caagcaaaaa
     2941 ctgtaaagac ttaaaagagg aaagtagaaa tggcgaagtg aagctaatca tgctataatg
     3001 ttaaagaatt gattgggata gatgccttct gtcgcaatca gactatttta tcacgaagaa
     3061 gatgatgcta cgaaaggaat ttaaactcta tggctactat tcaatggttt cctgggcaca
     3121 tgtccaaagc cagaagacag gttcaggaaa atgtaaagca tgttgacttt gtaactattt
     3181 tagtggatgc acgcttacca ctttctagtc aaaatcccat gcttacaaaa attgttggtg
     3241 ataaacctaa attaatgatt ttaaataaag ctgacttggc agatgcaact cgaactaaag
     3301 agtggaaggc ttattatgaa agtcaaggta ttaaaacatt agccatcaat tccaaagaac
     3361 aatcaacggt taaaaaagta acagaagcag ccaaggaact catggctgat aaaattcaac
     3421 gcttgcgaga gcggggcatt caaaaagaaa ccttgcgaac catgattatt ggcattccaa
     3481 atgctggtaa atctacctta atgaatcgct tggctggtaa gaaaattgct gtagttggca
     3541 ataaacctgg tgtgactaaa ggacaacaat ggttgaagtc caataaggaa ttggaaattc
     3601 tagacactcc tggtatttta tggccaaaat ttgaggacga attggtgggc ttaaaattag
     3661 ctttaacagg tgctatcaag gaccaattac taccaatgga tgaggtcact atttttggtt
     3721 tgaattactt taaaacacat tatcctagtc gccttactaa gcgttttaag aatattcctc
     3781 ttgaggaaga agctcctgaa atcattatga ccttgacacg ccaactggga tttaaagatg
     3841 attacgaccg tttctacact ttattcgtca aggaagttcg tgatggtaaa ttaggtcaat
     3901 acaccttaga tcaggtggga gatatggatg tcgactagta ttaaagctat taaagaaagc
     3961 ctggaagctg ttactagcct cttggacccc ctctttcaag aattggcaac cgacactagg
     4021 tcaggcgtcc aaaaagctct aaaaagccga caaaaggcta ttcaggccga gttagcagaa
     4081 gaagaacgat tagaagccat gctttcttat gaaaaagctc tttataaaaa aggttatcaa
     4141 gccattgcag gtattgatga ggtgggacgt ggtcccttag caggtcccgt tgtggcagct
     4201 tgtgtgattt tacctaagta ttgtaaaatt aaaggcctta atgattctaa aaaaatccct
     4261 aaatctaagc atgagaccat ttatcaggca gtgaaagaaa aggctttggc tatcggtatc
     4321 ggtattattg acaatcagct tattgatgag gtcaatattt atgaagcaac caaactagct
     4381 atgctagaag ccattaaaca gttagagagc caactcacac agccagatta tctcttgatt
     4441 gatgccatga cattggatat tgctatttcg cagcagtcta ttcttaaagg cgatgccaat
     4501 tccttgtcta ttgcagcagc atcaattgta gctaaggtca ccagagatca gatgatggct
     4561 aactatgatc gcatttttcc tggttatggc ttcgctaaaa atgcaggcta tggcaccaaa
     4621 gaacatttac agggattaaa agcttacggc ataacgccta tccatcgtaa aagttttgaa
     4681 cctgttaaat ccatgtgctg cgattcaact aatccttaaa aagttctgtt tttagcagaa
     4741 tttttttctt ttttacgaat aaatgaagta aggaggtaac ttagtgaatc attttgaact
     4801 ttataagcta aagaaagctg gactgactaa taaaaacatt ctcaatattc ttgactacca
     4861 agaacaccag gaaaaatcgt tgtcacttcg agatatggcc gttgtttctg gttgtaagca
     4921 tccgtctcac tttatagaag cctataagca gttagatatt caaaatttaa aaatagaatt
     4981 taaacagttt cctagtattt ctatcttaga taagcattac ccaatggcat tgaaagaaat
     5041 atacaatcca cctgtcctct tgttttttca gggaaattta gaccttcttg agaaacctaa
     5101 attagccatt gtcggctcca gacgctcaag cgataccgga gtaaagtctg tccgtaaaat
     5161 tcttaaagaa ctcgggaacc gttttgtgat tgttagcgga cttgctcgag gtatcgacac
     5221 tagtgctcat ttagcctgcc ttaaaaatgg gggacaaacc atcgctatta ttggaacagg
     5281 gttggatcgc ttttacccta aagaaaatag ggagttgcaa actttcttag ggaaaaatca
     5341 tcttgtgcta acagaatacg gtccaggaga agaagcttta tcttatcact ttccagaacg
     5401 gaatcgtatt atcgcaggcc ttagccgagg tattcttgtc gttgaagcaa aaaatcgttc
     5461 aggttccttg attacttgtc aaattgggat agaagaaggc cgagacattt ttgctgtccc
     5521 aggaaacatt ttggacggga aatccgaagg ttgccttcag ttaattaaag agggagcaac
     5581 atgcgtcacg tcgggcatgg atatcctttc agagtactaa aaaagttaaa gtttcttcct
     5641 atgtagcatt tttttattga cagtttctta aaaatgggat attatgctat aagctttaaa
     5701 caaactgtta taggaagtgt gtcgaattgg gaacaaaaac tacaactaag ccaaaaacgg
     5761 gtactaaaaa gtccaccact aaaaagaaat caatagcgaa aaaaaatctc gttattgttg
     5821 aatcacctgc caaagctaag actattgaaa aatatcttgg acgcagttat aaagttgttg
     5881 cttcggtagg acatattcgt gacttaaaaa aatcgtctat gtctattaat tttgataata
     5941 attatgagcc acaatacatt aatatccgtg gtaagggacc ccttatcaat tctttaaaaa
     6001 aagaagccaa agctgctaaa aaagtttatt tagctagtga cccggatcgt gagggagagg
     6061 ctatttcttg gcatttgtca catatcttag gacttgatcc tcaagataat aaccgagtgg
     6121 tttttaatga aattacgaaa gatgcagtta aacatgcctt tgtggagcct cgtcagattg
     6181 atatggactt ggtagattct caacaagcac gtcgtgtttt ggaccgtatt gtgggatatt
     6241 ccatttcacc aattctttgg aaaaaggtca aaaaaggact ttcagcagga cgtgtccagt
     6301 cggttgcttt aaaactcatc attgaccgtg aaaatgacat caaagcattt gttccaaagg
     6361 aatactggtc tattgatggt ctttttaaaa aaggaactaa aaagtttcaa gcgacttttt
     6421 acggtattaa tggcaaaaaa acaaagcttg ataataacaa cgatgtcaaa gaggtattag
     6481 caaagctaac aaatgaagat tttcttgtca gcaaggttga caagaaagag cgacgtcgta
     6541 atgctccctt accttacaca acatcatctc ttcaacaaga tgctgctaat aaaatcaatt
     6601 tcagaacgcg taaaaccatg atggtagccc aacaactgta tgaggggatt catttaggag
     6661 aaaatggaac tcaaggttta attacctata tgcgtactga ttctactcgt atcagtccag
     6721 tagcacaaaa cgatgctgct caatttatta tcaatcgctt tggagctaat tattcaaaac
     6781 atggtaatcg tgtcaaaaat accagtggtg cccaagatgc tcacgaggcg attcgtccat
     6841 ctagtgttaa tcatacgcca gactctattg ccaaatacct caacaaggat cagttaaaac
     6901 tttatacgct tatttggaat cgttttgtgg ctagccaaat gacagcagct gtttttgata
     6961 ccgtaaaggt gaacttggag caaaatggcg tgatttttgt tgccaatggt agccaaatga
     7021 agtttgatgg ttacatggct gtttacaatg attccgataa aaacaaaatg ttacctgaaa
     7081 tggaagaagg agaaaacgtt aaaaaggtat caacttctcc agaacagcac ttcacgcaac
     7141 ctccggctcg ctactccgaa gcaactctga ttaaaacttt agaagaaaat ggggtcggtc
     7201 gaccatcaac ttatgcccca accttagaag tgattcaacg caggtactat gttaaattat
     7261 ctgctaagcg ctttgagcca actgagcttg gggaaattgt caacaagctt attgtcgaat
     7321 ttttcccaga tattgttgat gttgccttta ctgccgagat ggaaggtaag ctagatcaag
     7381 ttgaaatcgg tgaagaagaa tggcaacatg tcatcgatca attctatcaa ccctttgtca
     7441 aagaattgaa taaggctgag tctgaaattg aaaagattca aatcaaagat gagccagctg
     7501 gttttgactg tgatgtttgt ggccacccaa tggtcattaa gttaggacgt tttgggaaat
     7561 tttacgcgtg tagcaacttc ccagagtgcc gtaatacaaa agccatcact aaagaaattg
     7621 gtgttacttg tccggtttgt cataaaggac aagttatcga gcgtaaaacc aaaaaaaata
     7681 ggatatttta tggttgtgat cagtatccag attgtgagtt tatctcgtgg gatttaccta
     7741 ttggacgtgc ttgccctaag tcaggtgact atcttattga gaaaaaagtt cgtggtggca
     7801 aacaagtaat gtgtagcaat gagacctgtg attacaagga agaaaaaatt aaataaaaaa
     7861 agaattggaa ctccttgtct tgttccaatt ctttttttat ttacaaaggt atttttaact
     7921 acaaaaatcg ctagttcgtt tttaaaaggc ttaaaaattc ctcaataact ggactgaaac
     7981 tcgcatcatc tcggtaagca atcccaatag ggtaaaaatt atttttatct tgcaaaggaa
     8041 tccaaactaa gttatctacc tgattaagac tctcaaaatc actcgggagg atagtcaagg
     8101 tatttaaatc gtgcaaacta tgaataagaa cttcccaatc attgtgttta aagacaatat
     8161 ctggttcaaa accgagagct cggcattttc taggaagcat ttcgcctaac atgtagtgat
     8221 cgtttaaaga agcaatctta tagcctttta aatctttaag ttttaactga ggatgttgcg
     8281 ccaaagggtg ctgtttgagg agaacgatac tgaccttata gccttttgtg gacgtttgta
     8341 atagctcaat ggtaatatcc ttttgaatac ttagaaagga gaggagacca atatcgattt
     8401 ttccttggga caataattct tataatttgc gagaaccatc ctgtataagt gatacttcaa
     8461 catttgagtg agtagccata aagattgaaa tttgtttcat gaattgtata gcaaacagtg
     8521 atgttaattc aacacgaatc atctcttttt ttcttgattt aattgctgaa tatccgcaac
     8581 aatttgatta aagtcatcca caaggtcttt gcctttgtga taaagaatat ctccagcttc
     8641 ggttagtagt agctgtcaat cactttgtgt aaaaagcgtg gtgttcaaac taggactcta
     8701 gccttttaat tgattgagaa agagtatgtt gcgttacaaa caaactttta gcagcgtggg
     8761 aatgattttt tgtttcagca actgcaataa aataggttaa ttatctaata tccatagtag
     8821 atccttcatg attagtcagg gaattgcgtc tctacttggt ttacaaccta ttttgatgtc
     8881 tctccttatt gccatgattt tctgcttcct tattgtttct ccaattacaa cggtaggaat
     8941 tgctcttgcg attaacttat ctggtattgg ttcaggtgct gcaaacttag gtatttgtgc
     9001 cgcaagcttt ggactttgtt tagcaggttg ggctgttaat tcaaaaggaa cttcacttgc
     9061 tcacgtcttg ggatcaccga aaatttcaat ggccaacgtt ttatcaaaac caaaaatcat
     9121 gttgccaatg ttatgttctg cagctgtact tggtgttatt ggtgctatct ttaatattca
     9181 aggaacacct gctagtgcag gctttgggat tagttgtttg atcggaccta tcaatgccct
     9241 taatttagct aaaggcggat ggtctctagt aaatatccta cttatcatta tcatttttgt
     9301 aagggcacca attgttctca atatgatctt taactacctt tttatcaagg tgcttaaagt
     9361 gattgatcca atggattaca aacttgatat ctaagaaagg aacttttgat gaaattaaaa
     9421 ttatataatg ttagaggcga agaagcagtc ttagcaaaaa aatgggctga tgacaatggg
     9481 attgagattt ccttgacaga gtcttcctta actcctgaaa cggttaaaga agctgaaggc
     9541 tttgacggga ttgccaatgc ccaaattggt cctttagatg atgcaatcta tcctctctta
     9601 aaggaaatgg ggattaaaca aatcgctcag cgcagtgctg gtgtcgatat gtataacctt
     9661 gatttggcca ccgaaaatga cattattatc acaaatgttc ctagctattc tccagaatcc
     9721 attgcagaat ttacggttac tattgccctt aatttaattc gtcatgtgga gttgattcgt
     9781 gaaaatgtta aaaaacaaaa tttcacttgg agactcccta tccgcggccg tgttttaggt
     9841 gatatgacag ttgcgattat cggaactggc cgtattggtc tagctactgc taaaatcttt
     9901 aaaggttttg gctgcaaggt cgttggatat gatatttacc aaagcgatgc tgccaaagct
     9961 gtcttggact ataaggaatc tgtagaagaa gcgattaaag atgctgatct tgtttcctta
    10021 cacatgccac caactgcaga gaacacacac cttttcaatg ctgatttatt taaatcattc
    10081 aaaaaagggg ctattttgat gaatatggcg cgtggtgctc tcattgagac gcaagattta
    10141 ctggatgctt tggatgcagg cttattgagc ggagctggta ttgacactta cgaatttgaa
    10201 ggaccttata ttcctaagaa ttttgaaggt caagaaatta cagattcact atttaaagct
    10261 ttgattaacc atcctaaggt tatttatacg cctcatgcag cttattatac tgatgaagcc
    10321 gttaaaaacc tagttgaagg tgcccttaat gctacagtag agattatcaa aactgggaca
    10381 acgacaactc gtgttaacta acaactgatt tgacgtccat tataaaggaa taattccaaa
    10441 aattccttta taatggactt ttttgtatta gtccgttata atagactgta agaaaggggg
    10501 ctcttatgac atatatagcg cttattggag atattatcca gtccaaacaa ttgacggatc
    10561 gttccaaagt tcaaaaaact ttagcagctt atttagacga cctcaacaaa acctttgctc
    10621 cttatattat ttctaaatta tccctaacgt tgggagatga atttcaagga ctttttcagg
    10681 tggatactcc tattttccat ctgattgatt tgattaatca tcacatggat atccctattc
    10741 gatttggagt aggagtaggt tctattctaa cagacatcaa tcctgatatt agtattgggg
    10801 ctgatggacc tgcttattgg catgctcgtg aggctattcg ttatattcat caaaaaaatg
    10861 attatggtaa tactacatta gctcttcgga ctggtcatca caaccaggat gatgtattaa
    10921 acagtttact agcagctgga gatgctatta aagccaactg gcgagctagt caatgggaaa
    10981 tttttgacac gcttttagat ttgggcattt atgaggaata ctttgatcaa caacgactag
    11041 gaaagcaatt atctcttagc tctagcgctt tgtcaaaacg tctaaaaagt agtcatgtta
    11101 aaatttatct gagaacacgg cagagtgcgc taaactgtct caaacaaatc aaggaggaaa
    11161 ctgatgatta atggtgtctc tcactaccta gctcaaaccc ctacacttac cctgtttctc
    11221 atttgtcatg tgttatcaga ctaccagtta caaagtcagc aagtagctga tttaaaagaa
    11281 aaacatttga cttatttagg ttaccacttg attggagtta gcattcccct aatttgttta
    11341 accctcatta tcccacaagc atggttgatg agcctattgg ttatgataag tcacgcgctt
    11401 attgactggc taaagccaaa gatggctaac tctctaaaat ggaaaagaga gtggattttt
    11461 ctgctggatc aatgcttaca tatcgctatt agttcatttg ctgggctaag gttagcaggg
    11521 gtgaccttgc ctaattggct tccgatatct attttgatga ccgttttatt tatcctagtc
    11581 attaccaaac caactaatat tgtctttaaa ctctttttca gcaaatacca gcctgaccaa
    11641 ggagaaaaaa tggacacgat cataggagcg ggggctacca taggaattct agagagaatt
    11701 gttattggtt tgtgtatgat catggggcaa tttgcttcaa taggtctggt ctttaccgct
    11761 aaatctattg ctcgctacaa taaaatttcg gaaagtccag cttttgcaga atactacctg
    11821 attgggtctt tatttagtat tctcagtgtt tttatcgcag catggatttg tttcttctag
    11881 ttgaaaaggg ttcccgtaga aagaggttat cgcttagtct ctttctcttt ctctttcttt
    11941 ttctttcttc ttatttctgt tataataagt caggtaacta cattagaaag atttgtgggt
    12001 ctgtcatcta agagatgtca ttcccgaaga ggtattagtg acttgtctca atcaactgca
    12061 acttatatta atgttattgg agctgggcta gctggttctg aagctgccta tcagattgct
    12121 aagcgcggta tccccgttaa attgtatgaa atgcgtggtg tcaaagcaac accgcaacat
    12181 aaaaccacta attttgccga attggtctgt tccaactcat ttcgtggtga tagcttaacc
    12241 aatgcagtcg gtcttctcaa agaagaaatg cgtcgcttgg attccattat tatgcgtaat
    12301 ggtgaagcta atcgtgtacc tgctggagga gcaatggctg ttgaccgtga ggggtatgca
    12361 gagagtgtca ctgcagagtt ggaaaatcat cctctcattg atgtcattcg tgatgaaatc
    12421 acagaaatcc ctgacgatgc cattacggtt atcgcgacgg gaccgctgac ttcggatgcc
    12481 ctggcagaaa aaattcacgc ggtaaatggt ggcgatggat tctatttcta cgacgcagca
    12541 gcgcctatca ttgataaatc tactattgat atgagcaagg tttacctcaa atctcgctac
    12601 gataaaggcg aagctgctta cctcaactgc cctatgacca aagaagaatt catggctttc
    12661 catgaagctc tgacaaccgc agaagaagcc ccgctgaatt cttttgaaaa agaaaagtat
    12721 tttgaaggct gtatgccgat tgaagttatg gctaaacgtg gcattaaaac catgctttat
    12781 ggacctatga aacccgttgg attggaatat ccagatgact atacaggtcc tcgcgatgga
    12841 gaatttaaaa cgccatatgc cgtcgtgcaa ttgcgtcaag ataatgcagc tggaagcctt
    12901 tataatatcg ttggtttcca aacccatctc aaatggggtg agcaaaagcg tgttttccaa
    12961 atgattccag ggcttgaaaa tgctgagttt gtccgctacg gcgtcatgca ccgcaattcc
    13021 tatatggatt caccaaatct tttaaccgaa accttccaat ctcgtaacaa tccaaacctt
    13081 ttctttgcag gtcagatgac tggagttgaa ggttatgtcg aatcagctgc ttcaggttta
    13141 gtagcaggaa tcaatgctgc tcgtttgttc aaaagagaag aagcgcttgt ttttcctcag
    13201 acaacagcta ttgggagttt gcctcattat gtgactcatg ccgacagtaa gcatttccaa
    13261 ccaatgaacg tcaactttgg catcatcaaa gagttagaag gcccacgcat tcgtgacaaa
    13321 aaagaacgtt atgaagctat tgctagtcgt gctttggcag atttagacac ctgtttagcg
    13381 tcgctttaac actaaaaaag agttcctttt cagttgagtt gctgaagagg acttttttac
    13441 ttgctgattt gagcgctagc tatgcaaaag aatgcacaaa aaatacatga atagactatg
    13501 gtcactagaa gcaatattat ataatgaata gtactcatta gttgattcag tttggaggtt
    13561 ttagcatgca gcagcaagtc gcaataacag aaacagtttt acgggacggt catcaaagtt
    13621 tgatggcaac gcgtttatct attgaggata tgcttcctgt ccttaccatc ttagacaaga
    13681 ttggttatta ttccctagag tgttggggag gagcaacctt tgatgcctgt attcgttttt
    13741 tgaacgaaga cccttgggaa agactgcgca cgttaaaaaa aggcttacct aatacccgtt
    13801 tacaaatgtt attaagaggc caaaaccttt taggctaccg tcattatgca gatgacatcg
    13861 ttgacaaatt cattagttta tcagctcaaa atgggattga tgttttccgc atttttgatg
    13921 ccctaaatga cccacgaaat atccagcaag ccttaagagc tgtgaaaaag acgggtaaag
    13981 aggctcaact gtgtattgcc tatacaacta gcccagtgca cacgcttaac tattaccttt
    14041 ctttagtgaa agaattggtt gagatgggag ctgattctat ctgcatcaaa gatatggctg
    14101 gtattctaac tcccaaagcg gctaaagagt tagtatctgg tatcaaagcc atgactaact
    14161 tacctcttat tgtgcatacc catgctacca gtggtatttc agaaatgact tacttaaaag
    14221 tagcagaagc aggagctgat attattgata cggcgatttc ctctttttca gggggaacca
    14281 gtcagcctgc cacagaatca atggcgattg ccttgacgga tttaggcttt gacacaggct
    14341 tggatatgca agaggtagcc aaagttgcag aatatttcaa taccattcgt gaccattacc
    14401 gagagacagg gattttaaat cccaaagtca aagacactga gccaaaaaca ttgatttacc
    14461 aagttccagg tggtatgcta tcaaacctat tgagccaatt agccgagcaa ggcctagctg
    14521 ataaatacga agaagtctta gcagaagtac ctaaagtaag ggctgatctt gggtatccgc
    14581 cactcgtaac gccattatca caaatggttg gcacacaggc cttgatgaat atcatctcag
    14641 gggaacgcta caaggtagtt ccaaatgaaa ttaaagacta tgttcgaggg ctatacggtc
    14701 aatcaccagc gccattagca gaaggtatca aagagaaaat cattggtgac gaagcagtca
    14761 ttgcttgcag accagccgac ctaatcgagc ctcaaatgat ttatctacgt gacgagattg
    14821 ctccatacgc tcgttcagaa gaagacgtgt taagctatgc gagtttccca cagcaagcca
    14881 gagatttctt gggacgccgt gaagatcctt tctatgatgt tccggttcaa gaagttactg
    14941 tacaactgga cattcaagac taacagggaa tgactaatcc tcttaaccat taatctcctt
    15001 ggtgacacgc atttgctgta agaacaatac caaaaaccta cctgattttg ataatcagat
    15061 aggttttttt atcttagtaa tgtttgacag atgaagtggc ttgtatcttt ttaataatgg
    15121 gttgggcttc tagcgaacaa aaataatcta aagaagtttt gggaaggagt tttgccagaa
    15181 ggtcccaatc attatctttc aatgcctgcc tagccgttga ggcactgata ggatcttgct
    15241 gctgattgat agctttcctt ggcaaaatat ctaggaggat accagcttgc tgcaactgct
    15301 ctgccatgat ttcattgtaa atagcagtta ctcggctggt tggctcttct ccaacgtatc
    15361 tttttgtaat ctgtaaggtt tgagcaatct ttagaaaaat agcagtatcc agcaaagctt
    15421 ggcttttgat cacatcattg tcttccgttt ggaaataagc tggaaaggtt gcctgactaa
    15481 tcaaataagg acctgtttca tgaaagataa tgttatcaag atgtctcaat ccttcttgaa
    15541 tcaacatttt tcgtactgaa aaaggaacca agctttggtc ctcactaacc ataaacagat
    15601 gaacccaatc attttctctt gcagctttct ctactaaaaa ttgatgccct aaggtaaagg
    15661 ggttagcgtt gataacaata gcagcgactt taccaggtgt ttgctcaggc ttattaagtg
    15721 tcatcagata atcttgaaaa ccagtttttt tattttccaa gaaactgatg tgattgtcga
    15781 tatggacaat ttcagtaaaa ccaagatctt tgaaaaaagc agcagaagag gtcttcgtat
    15841 aaacaaaaag atgatagttg ccaagggcat attcttcatc aatgagatga ctcacaatcc
    15901 tattaagaag tccttcacct tgataggcac tggatacgca aagacagcgc aagctatttc
    15961 caaagtaaga accggtagca atcacatcat tttgcgcatt catgatggca caagtataat
    16021 ctaaatgagc atccaaacga ataccttctt gatgaagcag gttctttaca ctagccattg
    16081 tcgttttatc tgaaggaaag acttttgaaa cagtgtagta aggcatttct tctccttatc
    16141 tttaatttta ttgaatcata acataactta gtaccaaaac aggctgatct atctaaaggc
    16201 aactctgttt tttatcaacg ccatttttta aaaggaaatc gaatgtgttt gacagactca
    16261 aaagtatcag cgattttttg attgcgttcc acagtccgca agttttcttg gtctaataag
    16321 tcttgacttt tttccaaaac atcaacagta atgtagtaga gtttgatcag gcttcctatc
    16381 ttatatttac cagattttgg agacatagca accacttcat tgagattact atgaagccat
    16441 tttttatgag gcttagcagg tatagtggct actacaaagc caagttgttc caaatgagct
    16501 tgtgcctgat caacaggaag attaatcaca tcatctatcg tgcgtagttg catctctttt
    16561 tcatgttttt gttccatacg tttttcgata attggtcgac tgttatcaat cgtttttcca
    16621 ataatttcag tagtgtcagg aacaattgac atcagtttcc caactactcc tagagctttt
    16681 cttattctct ttggcatctt ttatctcctc aagtcgtttc tttttattat aacaaattgt
    16741 ctaaaaatag tatgcgataa accttgttct aaaagattaa atagatcaat tcaaggaaaa
    16801 aataggaggg gatccgacaa aaaatacaaa gattctaaaa aagtcaagct attttagcct
    16861 ttgtcaatcg tatcagttca aatagaaaat acaagttttg acaagaattt atttatgtaa
    16921 caaactttgt ttttgattct tcagactatc tagcgataat gttatctgat tcttgttttc
    16981 aaccaattga tgttaaagaa gctgtcttca tgattctcta acattccttc agttttagct
    17041 ttata
//
LOCUS       DAUGHY010000035        16863 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.035, whole genome shotgun sequence.
ACCESSION   DAUGHY010000035 DAUGHY010000000
VERSION     DAUGHY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16863
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.035"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            195..644
                     /locus_tag="VH752_001224"
     CDS             195..644
                     /locus_tag="VH752_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEQ8289578.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSDQFALTGAVKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
     gene            819..1103
                     /locus_tag="VH752_001225"
     CDS             819..1103
                     /locus_tag="VH752_001225"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEQ8289579.1"
                     /translation="MRLEEIRQEINGIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QARENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRQYQTQRLTGGDIND"
     gene            1096..2358
                     /locus_tag="VH752_001226"
     CDS             1096..2358
                     /locus_tag="VH752_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEQ8289580.1"
                     /translation="MIKSIATLPKPSLKEALGLIGLALPIGATVGVVDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKDQIPPILIPL
                     ILFSTWVTHLFGASAGREGVAVQIGATISHYCQRFVTSQEAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYCALLPSLVAACVASWTSHSLGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFAYLLGWFKPYLSQKLPNPYFRIAFIGALLSICLMIGHV
                     GRYSGLGTHLIAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGIV
                     LAPYLGLPVLLVAALGYTTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMVS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            2473..2820
                     /gene="rplS"
                     /locus_tag="VH752_001227"
     CDS             2473..2820
                     /gene="rplS"
                     /locus_tag="VH752_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEQ8289581.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            3067..3138
                     /locus_tag="VH752_001228"
     tRNA            3067..3138
                     /locus_tag="VH752_001228"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3100..3102,aa:Arg,seq:cct)
     gene            complement(3101..3415)
                     /locus_tag="VH752_001229"
                     /pseudo
     CDS             complement(3101..3415)
                     /locus_tag="VH752_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004234471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3823..4392
                     /locus_tag="VH752_001230"
     CDS             3823..4392
                     /locus_tag="VH752_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEQ8289582.1"
                     /translation="MKGMNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAV
                     YQKLKESTAIAVAMFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNF
                     LISHRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGD
                     RLIDKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            4393..6345
                     /gene="gyrB"
                     /locus_tag="VH752_001231"
     CDS             4393..6345
                     /gene="gyrB"
                     /locus_tag="VH752_001231"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEQ8289583.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
                     ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNHGLKISITDKR
                     SGMEQEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRDFIEENAVYSTLDI"
     gene            6737..8461
                     /gene="ezrA"
                     /locus_tag="VH752_001232"
     CDS             6737..8461
                     /gene="ezrA"
                     /locus_tag="VH752_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEQ8289584.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKRNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLSEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(8593..9051)
                     /locus_tag="VH752_001233"
     CDS             complement(8593..9051)
                     /locus_tag="VH752_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEQ8289585.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKQTPEKTKSFWKRFLKK"
     gene            9284..10591
                     /gene="eno"
                     /locus_tag="VH752_001234"
     CDS             9284..10591
                     /gene="eno"
                     /locus_tag="VH752_001234"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEQ8289586.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMITLDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQV
                     DYLEELVNKYPIITIEDGMDENDWDGWKVLTERLGKRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(11066..12097)
                     /locus_tag="VH752_001235"
                     /pseudo
     CDS             complement(11066..12097)
                     /locus_tag="VH752_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677767.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12364..12924)
                     /locus_tag="VH752_001236"
                     /pseudo
     CDS             complement(12364..12924)
                     /locus_tag="VH752_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            12957..>16863
                     /locus_tag="VH752_001237"
     CDS             12957..>16863
                     /locus_tag="VH752_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEQ8289587.1"
                     /translation="MLQTVTVTKDQQNPISITLSEDQAKSLKNKEKLKVSIKQKQSKK
                     TSKDFFFEVGIDPKVEAKQKEKLLELDKVKKQIEDSINGDAWLPEKPEGEKPVQNTNK
                     ELQLQELNKKYQMAKEAIESATTLDDVETQFDKYTKVGDKDKYPDSLRNQYTQGDKDK
                     EIEKAKKSLGDLSDKVNGKIEEDKWLSAEVKKKQQQELEARKQKVNDSLKGSDSLKSL
                     RETVEKASSKNQKKPESFEDVYVPGNEETEKTKVRDILQKTYQKTEQNIETDPWLSPE
                     QKKAQKENAKTRLDAGLKAVETTESLDKLKEVESDFLDKEKNPDSIPNQHKAGTPEVA
                     RKTFLDNFEKEAKKEIESIKNDVTLTDAEKATAKAKVEAQLQEAKGKAKESKSFDDLK
                     NIQDKFNSELPHTPGKPLKDQQSDAIAELEKKQQEIEKAIEGDKTLPRDEKEKQIADS
                     KERLKSDTQKVKDAKNADAIKKVFEEGKVNIPQAHIPGDLNKDKEKLLAELKQKAADT
                     EKAIDSDKTLTAKEKEEQKAKTKEELQKATEAVGAIDNREELDKKVPELKKAIEDTHV
                     KGNLEGVKNKAIEDLKKVHDETVAKINGDDTLDKATKEAQVKEADKALAAGKDAITKA
                     DDADKVGAAVTEHTPKIKAAHKTGDLKKVQEEANQALDKAAEKERGEINNDITLTAKD
                     KEQQLKEVETALTKAKDNVKAAKTADAINDARDKGVATIDAVHKAGQDLGARKSGQVA
                     KLEEAAKATKDKISADPTLTSKEKEEQSKAVNAELKKAIEAVNAADTADKVDDALGKG
                     VTDIKNQHKTGDPVVARREAHGKQLDRVAQETKDAIEKDPTLTTEEKAKQVKDVDAAK
                     ERGMAKLTEAKNADELDKAYGEGVTDIKNQYKSGDPIEARRGLHNKSIDKVAQATKDA
                     ITADTTLTEAEKETQRGNVDKEATKAKEELAKAKDADALDKAYGDGVTSIKNQHKSGK
                     GLDVRKDEHKKALEAVAKRVTAEIEADPTLTPEVREQQKAEVQKELELATDKIAEAKD
                     ADEADKAYGDGVTAIENAHVIGKGIEARKDLAKKDLAEAVAKTKALIIEDKTLTDDQR
                     KEQLSGVDTEYAKGIENIDAAKDAAGVDKAYSDGVRDILAQYKEGQNLDDRRNAAKEF
                     LLKEADKVTKLINDDPTLTHDQKVDQINKVEQAKLDAIKSVDDAQTADAINDALGKGI
                     ENINNQYQHGDGVDVRKATAKGDLEKEAAKVKALIAKDPTLTQADKDKQTAAVDAAKN
                     TAIAAVDKATTADGVNQELGKGITAINKAYRPGEGVKARK"
BASE COUNT         5555 a         2896 c         3535 g         4877 t
ORIGIN      
        1 tggtaaatta atctaggaag aaaagaggtt agttatcagt tatgatacta tcaaagctag
       61 ttttgcaaat gcaagtgaaa aaatagtttt taagtgtctt aagtaatcat atcaactttc
      121 tctttaaaca attagtgctt ttcctaaaaa agtgtgctaa aataaacata gtttagtaat
      181 tttgggagga atttatggca ttagcaaaaa tcgtttatgc cagcatgacg ggaaatactg
      241 aagaaatcgc tgatatcgtt gcaaataaat tgcaagaatt gggccatgat gttgacattg
      301 atgagtgcac aacagttgac gcttcagagt ttgagaatgc tgatatcgct gtcgttgcga
      361 cttatactta tggtgacggt gacttaccag atgaaatcgt tgatttttac gaagatttac
      421 aagatttaga tttagaaggt aaaatctatg gcgttgtcgg ctctggtgac actttttatg
      481 actatttttg caaatctgtt gatgactttt cagatcaatt tgcgcttact ggtgccgtca
      541 aaggtgcaga gccagtcaaa gtagatttgg cagcagaaga tgaagacatc gatcgtttag
      601 aggcctttgc agagcaactc tcacaggctg ttaataatct ataaaactat ttagtaaaaa
      661 tgtcagggac ttccttggca tttttagatt tttaacctaa aataaaggga atgtcacttc
      721 agggtaggaa ttgaaaaaac aatcagcttc accttgcttc tgatcctctt attaactata
      781 ataaaaagat aatctaatac atctagaaaa ggaaagttat gcgtttagaa gagatacgac
      841 aagaaatcaa tggtattgat caccacttgg ttgccttatt agaaaagcgg atggcacttg
      901 tagagcaagt aacagcttat aaactagcca atcacttgcc tgtcttagat caggcgaggg
      961 aaaatcagat cctagatagg gtttcttatc tcgttaaaga ccaagcattt gagccagcta
     1021 tccatgagac ttttaaaacc attatgtctc tttcacggca gtatcaaacg caacgcttaa
     1081 caggtggtga cattaatgat taaatcaatt gcaacactgc ctaaaccctc cttaaaagaa
     1141 gcgttaggac taataggctt ggcgcttccc ataggggcca cggttggagt ggttgatgtg
     1201 atttttggta aagggctttt gtttctttcc gaatatcgcg atcaccacct gttcttgctt
     1261 cttccttttc ttgccctagc aggtcttgtt atcgtttttc tttacgataa gttaggcaaa
     1321 gaggttcgtc aagggatggg attggttttt caggtagggc atggtcaaaa agatcagatt
     1381 cctcctattc tcattcctct tattttgttc tcgacttggg taacccactt atttggagcg
     1441 agtgcgggtc gagaaggggt agccgttcaa ataggagcta ccatctctca ttattgtcag
     1501 agatttgtga ctagtcaaga agccgcacga caccttttga ttatggggat ggcagccggt
     1561 tttgcaggac tttttcaaac gccgattgct gctgtcgtat ttgcccttga ggttttgttg
     1621 gtaggaacgc ttcgctactg cgctttattg ccttccttag ttgctgcttg cgtggcttct
     1681 tggacctctc attctttggg attagagaaa tttacgattg ttcttgaaga aacattgacc
     1741 ataactccct taacattggt taaattaatt gggttaggcc ttatttttgg tctggtggga
     1801 aatagttttg cctatttact tggctggttt aaaccttacc tcagtcaaaa gctaccaaat
     1861 ccttatttcc ggattgcttt tatcggtgct ttgttatcta tctgtctgat gattggtcac
     1921 gtcggaagat atagtggtct aggaactcat ttgattgcgg cagctttttc tggccaaact
     1981 atcctaactt acgactggct tttaaaaatg atcgtgacgg tgattagctt atctgcaggc
     2041 tttcaaggtg gtgaagtgac gcctctattt gctattggag cttctttagg catcgttttg
     2101 gccccttact taggtcttcc tgtactgttg gttgctgcac ttggttacac cacggtattt
     2161 ggaagtgcaa caaatacctt ttgggcccct atctttattg ggatagaggt ctttggtcca
     2221 gagaatgccc tagcatattt tgtcacctcg gctgcagcct atatggttag tcacagacat
     2281 tccatttaca gttatcaaaa agtagtcatg atcaatttaa aagacatgac ggtaaatcag
     2341 atggatttaa aaaaatagct ttacaataaa gaagcttttt gttagaataa atctcgtgtg
     2401 taatggacac acgaaaatta cggctaatcc gctaagacaa gtacttaaga ttagtaagat
     2461 aggagaataa aaatgaatcc attaatccaa agtttgacag aaggtcaact tcgctctgat
     2521 atccctaact tccgtcctgg tgacactgtt cgcgttcacg caaaagttgt cgaaggaact
     2581 cgcgaacgta tccagatctt tgaaggtgtt gttatttcac gtaaaggtca agggatctca
     2641 gaaatgtaca ctgttcgtaa aatctcaggt ggtatcgggg tagagcgtac attcccaatc
     2701 cacacaccac gtgttgacaa aatcgaagtt atccgccacg gtaaagtccg tcgtgcgaaa
     2761 ctttactact tgcgtgcatt acaaggtaaa gctgctcgta tcaaagaaat tcgtcgttag
     2821 tttgaaccga aacgtaacgt aatagaataa aaagactaga gtctctagtc tttttatgtc
     2881 gtcgtgacaa tgtgacaatt ggtgattaac gctatgctcg taggacactt tgaaagttcc
     2941 ttagcagtta taattagtat attgtaataa caaaatgaag caaaataaac tttcagttat
     3001 ctgatatttc atcttgcatt accttatttt ctaggttaaa atagaagaca aggtgaatat
     3061 aagataggcc ccttagttca atggatataa caactccctc ctaaggagta gttgctggtt
     3121 cgattccggc aggggtcatt attgtcactg aaaaagcctt tacatcaaag gctttttgtt
     3181 ttattccggt tttaaaaggg gcagtttgtt tatttataat ttctttcatg tttacagttg
     3241 tgtgactgta aatagataat gttgtttttg gatcgctgtg accaactcta tccattatcg
     3301 catttagcgg tatccctttt tctgctaaaa atgatatatg cgagtgtcta aataagtgcg
     3361 tgtgataatc tccgtaaatt ttcaatcgct tattgatgtg tgcatttaaa attggtaaac
     3421 cttttgaata tggaaagctg ttagcactct gcttgtgatt aatctatcgt atgttaaaag
     3481 tgacatttaa attaaatcat tttttataca tatatttcaa caggatttat catattggaa
     3541 aatagcggtg cttctattgg cattttacta ttgacaggaa tttccctgtc ttttgtctcc
     3601 taaagaagat cagctttgat ttctaacttg attagagtgg gcttggtact gtttatgaat
     3661 tatcagtata tactttctgg agatcaaaaa ggaaaggaac agctaaagag gtgtatgtgt
     3721 taccattaga ggaatagaac atcgcaagac tcgtttattc agtcattacg atacctgttt
     3781 tatttgggaa ataactataa tattaagtga atttgtgata taatgaaagg tatgaattat
     3841 caagattata tatgggattt gggaggtacc ttactcgata attacgagct ttcaacccaa
     3901 gcttttgtcc aaacgctagc ttttttcagc cttccaggag atcacgatgc tgtttaccaa
     3961 aaattaaaag aatcaaccgc tatcgctgtg gcaatgtttg ctcctaatga gccagaattt
     4021 ttgcatgtgt ataggctaag agaagcagac aaattggcac aacccatttg gtgtttggga
     4081 gctaaagaaa ttttggaaaa gatagcgact tcaggttcgc gaaatttttt aatttctcat
     4141 cgagattgtc aggtgaacca acttttagag caagctgggt tgctaatcta ttttacagaa
     4201 gtcgtgacag cttcaaatgg ctttgctcgg aaaccaaatc ctgaaagttt gttttattta
     4261 aaagaaaagt atgatattaa tagtggatta gtaattggag atcggctaat tgataaacaa
     4321 gcaggacaag cagcgggctt taataccttg cttgttgacg gtagaaaaaa cctattggag
     4381 atagtaacgt agatgattga agaaaataaa cattttgaaa aaaaaatgca agaatacgat
     4441 gccagtcaaa ttcaggttct agaagggctg gaggctgtcc gcatgcgtcc agggatgtat
     4501 attggctcga cagctaaaga gggtttgcat catttagtct gggaaattgt tgacaactca
     4561 attgacgaag cattagcagg ttttgcctct catattaaag tctttattga agcagataat
     4621 tccattacag tagtagatga tggccgtgga attccagttg acatccaagc caagacagga
     4681 cgtcccgccg tcgaaacagt ttttacagtc ttacacgcag gtggtaaatt tggcggaggc
     4741 ggctataagg tttctggagg attacatggt gtagggtcat ctgttgttaa tgctttatca
     4801 acacaattag atgtacgtgt ttataaaaac ggccaaattc attaccaaga atttaaacgc
     4861 ggggctgttg tagcagatct tgaggtcatt ggaaccactg atgtgactgg aacaacggta
     4921 cactttacac ccgatccaga aatttttacc gaaacgactc agtttgatta cagtgtttta
     4981 gcaaaacgta ttcaagagtt agccttttta aaccatggtt taaaaatttc cattacagat
     5041 aagcgttcag gtatggaaca agaagaacat ttccattatg aaggtggaat tggttcttat
     5101 gttgaatttt taaatgataa aaaagatgtt atctttgaaa cgcccatcta tacagatggt
     5161 gaattagaag gtattgcagt tgaagtagcc atgcaatata cgactagcta tcaagaaaca
     5221 gtcatgagtt ttgctaataa tattcatact catgaaggtg gaacgcatga acaaggcttt
     5281 agagcggctc ttactcgggt catcaatgac tacgctaaga aaaataaaat tcttaaagaa
     5341 aatgaggaca atttgacagg agaagatgtt cgtgaaggtt tgacggcggt aatttctgtt
     5401 aagcatccaa atcctcaatt tgaaggtcaa accaaaacaa aattgggcaa ctcagaagtg
     5461 gttaagatca ctaatcgtct ctttagtgag gcctttcaac gttttctttt ggaaaaccca
     5521 caagttgctc gtaagattgt ggaaaaaggg attttggctt ctaaagctag aattgcagct
     5581 aagcgagccc gcgaagtcac ccgcaaaaaa tcaggcttag aaatttcaaa cttacctgga
     5641 aaattagcag actgttcgtc aaatgatgct aaccaaaacg aacttttcat cgtcgaagga
     5701 gattcagcgg gtgggtcggc caaatcaggt cgtaaccgag agtttcaagc tatcttgcct
     5761 attcgcggta aaattttgaa cgtggaaaaa gcaactatgg ataagattct tgccaacgaa
     5821 gaaattagaa gtctctttac cgctatgggt acaggttttg gtgcagactt tgacgtgtca
     5881 aaagctcgct accaaaagct ggttatcatg accgatgccg atgtggatgg cgctcatatt
     5941 agaaccttgc ttttaacttt gatttaccgc tttatgaggc ctgttctaga agctggctat
     6001 gtttacatcg cccagccacc tatttatggt gttaaggtcg gtagtgagat taaagagtat
     6061 attcagccag gtattgatca agaagaccaa ttaaaaacag ctcttgaaaa atatagtatt
     6121 ggtcgttcaa aaccaactgt tcaacgttat aaaggtcttg gggaaatgga tgaccatcaa
     6181 ctttgggaaa ctactatgga tcctgaaaat cgtttgatgg cgcgtgtgac agttgatgat
     6241 gccgcagaag cagataaagt atttgatatg ttaatgggag atcgtgttga accaagacgt
     6301 gatttcattg aggaaaatgc ggtttatagt acactggata tttagtcacc tttttagttt
     6361 cacaaggaat ctgctaccaa agaggacgaa ttgactaaaa atcatgatgc atgatgccaa
     6421 cagctttgtt agttagtacg tcttcatctt tgttagttag tacgtcttca tcttgaggga
     6481 cagcgttaat gcataggtat tgagaccgcc ccttaatacc tatgttagtc cttattaaga
     6541 aagccttgtg agtcgctggt acgagattgt tgaattagtc aaatatattt tgaaatgcat
     6601 tttaaagtat gttataatcg taatgttata gtgtatttta aataaaattt taataaaagg
     6661 cgattgagga atttcagttt ttagttatta ctggaacaat gttgataaat cgtgtctaaa
     6721 taataaggag attcagatgt caagcggaat tatcttgctg attgtggcta tagtcctact
     6781 agtgattatc gcctatctag tgggtgtcat catacgaaag cgtaatgatt ccctaattac
     6841 cagtctagaa gaacgaaaac aggccttgtt tgctttgcct gttaatgatg aaattgaaga
     6901 agtaaagtca cttcatttga taggacagag ccaaacctct tttcgggaat ggaatcagaa
     6961 atgggtagac ttaactgtaa attcgtttgc agatattgaa aatcatattt ttgaagctga
     7021 aaatttgaat gatacgttta attttattcg cgccaagcat gagattaata gcgttgaaag
     7081 tcagcttaat cttgttgaag aagatatcgc ttctattcgt gaggcactta atatcttaaa
     7141 agagcaagaa gaaaaaaata gtgctcgtgt cacccatgct cttgatttat atgaaaagct
     7201 ccaagcttct atttctgaga atgaagataa ttttggttca actatgcctg aaattgacaa
     7261 acagatgaaa aacattgaaa ctgaattttc acagtttgtc gcactcaact catcaggtga
     7321 tcctgtagaa gcttctgaag ttctggatag agcagaagag catactattg ctctagggca
     7381 gattacagag cagattccag ctattgtcgc caagttggaa gatgattttc cagatcagtt
     7441 agatgatttg gaaacaggat atcgccgttt acttgaagaa aactatcatt ttccagaaaa
     7501 aaacattgag gcacgttttc aggagattcg tgagtctatt cgtgccaatt catcagagtt
     7561 agttacactt gacttggatc gtgctagaga agaaaacacg catattcaag aacgcattga
     7621 ttctctttat gaagtctttg agcgcgaaat tgccgcttat aaggtagctg ctaaaaatag
     7681 taagatgcta cctcgttacc tagcacatgt gaaacgtaat aatgaacaac tcaaagatga
     7741 aattgcacgt ttatcacgta aatatattct aagtgaaacg gaaagtctga ctgttaaggc
     7801 atttgagaag gatattaaag agatcgaaga cagtacactt gctgttgcag aacaatttgg
     7861 tttacaagaa aaaccatttt cagaactgca agtgactttt gaacgcagta taaagacgct
     7921 tactaatgtc gagtcaggtc aaatggatgt ttttgcagca gttaaagata ttgaaaaaat
     7981 tgaatcacag gcacgccata accttgatgt ttatgttact cagcttcata tgattaaacg
     8041 ttatatggaa aaacgtcact tgcctggaat tccacaagac tttcttagtg ctttctttac
     8101 aacgagttca cagttggaag ctttgatgga tgagcttagt agaggacgga tcaatattga
     8161 ggcagtgtca cgtttgtcgg aagttgcgac ggttgctatt gctaatctag aagatttaac
     8221 ctatcaagtg gttcaaaatg ctaccttgac tgaacaatta ctacaatatt ctaatcgcta
     8281 tcgttctttt gaagcaggag ttcaaagtag ttttgaacat gctttgagac ttttcgaagt
     8341 agaaaatgat tatcaggctt cttttgatga aatttcttat gctcttgaaa cagttgaacc
     8401 tggggtaaca gatcggtttg ttaactctta tgaaaaaaca cgagagcaca ttcgtttttg
     8461 atacttgaaa gtcgtgatat tctcacgact ttttattagc tttttggacc aatacgtgtt
     8521 tttgactaga atcttctttt taatgatcaa aagaagaagt agtaacagtg ttttttgcta
     8581 ttgagaggat agttattttt tcaaaaatct tttccaaaag gatttggttt tttcaggagt
     8641 ttgtttgcta agttgatcgt tttttgtctg ctttaagatg acttctaaac gcgtttcctt
     8701 tagatttatt gcgtggtcgg tatgaaggat aaacgcaaaa ggtgaagtca tatgcgtttc
     8761 tgtaataata gtaacaggta aatggttttc ttttgctaat ttcatataga gtatttgaag
     8821 ttgaggtgcc agcttggaag agatttttaa gtgtaaaggt tgatagacta actgaaggct
     8881 cggtaattct cttttcaaat agtcttttac tgttttagtt tgagcttcgt ctaataaagc
     8941 acttaaaacg actcgttcag catacgttcc cagatagtag cgttgctgct caggatttaa
     9001 tcttctttct ccccaggctc cttttaatag cttatcttct aaattagtca tagataccct
     9061 cgcttcttta taataataat agtttcttta ttatatcgat ttctacaata aaataccaat
     9121 ttgaaaattt tcggtaaatg tgttaaaaaa caagaaagaa agtgattttt cttgcaaaaa
     9181 acaaaggaag cttttcatga gattgctttt taagttgatt aaaggcctag tttgtggtat
     9241 tatgaacatg taaaatatta aaactcataa ggagagtatt acaatgtcaa ttattactga
     9301 tgtatacgct cgcgaagtcc ttgactcacg cggtaaccca acacttgaag tagaagttta
     9361 tacagaatca ggtgcattcg gacgtggtat ggttccttca ggagcttcaa ctggtgaaca
     9421 tgaagccgtt gaacttcgtg atggtgacaa atctcgttac cttggtctag gtactcaaaa
     9481 agcagttgat aacgttaata acattatcgc tgaggcaatc atcggttacg atgttcgcga
     9541 tcaacaagct atcgaccgtg caatgatcac tcttgacggt actcctaaca aaggcaaact
     9601 tggtgctaat gctattcttg gtgtttctat cgctgttgct cgtgcagctg ctgactatct
     9661 tgaagtgcca ctttacactt accttggcgg attcaacact aaagttcttc caactcctat
     9721 gatgaacatc atcaacggtg gatcacactc agatgcccca attgcattcc aagagttcat
     9781 gatcatgcca gttggtgcac ctactttcaa agaaggtctt cgttggggtg ctgaagtttt
     9841 ccacgctctt aagaaaattc ttaaagaacg cggacttgtt acagctgtag gtgacgaagg
     9901 tggatttgca cctaaatttg aaggaactga agacggtgta gaaactatcc ttaaagctat
     9961 tgaagcagct ggttacgaag ctggtgaaaa cggcattatg atcggttttg actgtgcatc
    10021 atcagaattc tacgacaaag aacgtaaagt ttacgactac actaaatttg aaggtgaagg
    10081 cgctgctgtt cgtacatctg cagaacaagt tgattacctt gaagagttgg ttaacaaata
    10141 cccaattatt actatcgaag atggtatgga cgaaaacgac tgggatggtt ggaaagttct
    10201 tactgaacgc ctaggcaaac gtgttcaatt ggttggtgac gacttcttcg ttacaaacac
    10261 tgaatacctt gctcgtggta tcaaagaaaa tgcagctaac tcaatcctta tcaaagttaa
    10321 ccaaatcggt actttgactg aaacgtttga agctatcgaa atggctaaag aagctggata
    10381 tactgccgtt gtatcacacc gttcaggtga aactgaagat tcaacaatcg ctgacatcgc
    10441 agttgcaaca aacgctggcc aaatcaaaac aggttcattg tcacgtacag accgtattgc
    10501 taaatacaac caactacttc gtatcgaaga tcaacttggt gaagttgctc aatacaaagg
    10561 tatcaaatca ttctataact taaaaaaata gacggtaaga cagaaatcgg tcattcgtag
    10621 aatgcgattt cgtcgtcgca cctccgtcag gctgactagg ttggtcacga caaaaggagt
    10681 ggacatccag tcagctacga cgcaaaagtt aaagaataga aagcttggga aaatcccaag
    10741 ctttttgctt tctaaatagt aaaaatttac tgtgggctaa tttatcctga gctaatgtat
    10801 tagcgaggta aagttactga agaagacttg gatttgtact gcctctaaaa agttggacac
    10861 tatattttaa atacaggact aagtcctttt ataccaattg tcaagcacat caaggtttta
    10921 actgaaaaaa tgttttagac aatagcctag gtttagctgt tgtcttactt gttgtgctct
    10981 gattagcata aaaaagtgta caagaaaaaa accttgccta gtatttttaa gatcaaggtg
    11041 ttacaatgat agagtaacaa cgactttaat gattttgggt cgaagcgtaa ttgtaaagtt
    11101 tgttatgcgt tatgaggtaa agcattgtcc tgacaaggca atgtctagag gcaatcgtat
    11161 gtggcttggt tgaagctgtt tacgattgcc tttttcgttt ctcatagaag tctgcgatat
    11221 gacaaggatt ggtatgacta gcagaagcaa tgtcatggat acacttgaaa agaatctttg
    11281 gagcataagg attcccacgt tcggtaatgt gctcttgagc tagacagttg ccagactcat
    11341 aatgtctaaa gtcaatccca atgaaagcat taatttgatt agcagattga aaacgacgaa
    11401 tagcaccgag ttcgtcaata atacttgttg cagttgtttc tgcgatactt ggaatagagc
    11461 gtagaatctt gtcttctggt aaaggttgag ccaacgccac catcttatca aaaatagctt
    11521 gacgacgttc agaaagtcta agtaattctt tggcataata gcgaacctct tctatcatcg
    11581 gagaggtttt cgtaacagta aaataagact gcttggctaa tgagacaagt ttttccacta
    11641 agtagatccc acgcttgtca gagatacgtt tagaggtaga ttgacggatg atagctgaca
    11701 atgctttgtc agctagctcc agtacaaagc tattacatgg aaaagccgtt accacattcc
    11761 agtattactc accagttggt gcaaataaga tgtgttccag ttcaggaaaa gtgacttgta
    11821 agatcttgtg aaggcgattc ttaactctcg aacaatatta tcctcagtct caagttttga
    11881 tagaagcgac tcaagtcacg tagcagctga taaacttctt cctggacgta agttggtttt
    11941 ctaactagaa taaattgaga ctgagccaat ttttcagtgt ctaacttatc cgctttacgc
    12001 acccgtagac tatctagttt tctaaaggat tgagttgtgt ataagcgtag ccatttttct
    12061 caaggaagga ctgaagccga cgagaataga ctcctgttgt gatggtctcc cttttgtctt
    12121 ttaaacaata gtatagccgt ttttcaaaat gttattaatg attttttcct ttattctaga
    12181 agaataagtc tgatactgtt tcttcttaat aatctcaatg ccctaacaat ccatcagtcg
    12241 aacggtattt ctcaaattac tgcagttgac tcttgcgttc agttccctca ccttgtggat
    12301 gccgtttaga acaatattag cttgctgagc cttttggtaa tgtgaacaaa gcatctttat
    12361 cactcaagga gaagacaggt tcttgtggta agtgtccaac aaggtcacga taaactcatc
    12421 gtaccgtttt tggcagaagt ctagaaatgc cttatgaatg taagcctcag aaacgtctct
    12481 aaaactaaac aagaaataca agaacaaatg cccttgattc tggtacaaat gctgtatcag
    12541 gttcaggtat tgcctatctg cgataatgac actctgcttt tccttaacaa tatctttgaa
    12601 aggaatgatt tcgacattga caggcataac cgttgcaata gctggcggga agtaaatcga
    12661 gagtgcattg gtcatcaaat ccagcgcagc agtgttattg gtcaaaagat agacaggtat
    12721 cggtggcaaa tatgttttta ggagttcctc taactgtatc gtgaagagca gtaaatgata
    12781 ggacggcagt ttccccacga taccttctgc gctcatccat tcctcaacga tggtttttag
    12841 agtattgtag aagctcttgt ttcgtttttg ctgtcttctg aggtaatgga acaagtgtat
    12901 atcaggtaac aaacttcgaa ggtgatgaga taagcctttt caactctagg ggagagttgt
    12961 tacagacagt tacagttaca aaagatcaac aaaatcctat atcaataact ttatctgaag
    13021 atcaagccaa atctttaaaa aataaagaaa agttaaaagt aagcattaaa caaaagcaat
    13081 caaaaaagac ttctaaggac tttttctttg aggtaggaat tgatcctaaa gttgaagcaa
    13141 agcaaaaaga aaaactcttg gaactagaca aagttaaaaa acaaattgaa gactcaatca
    13201 atggtgatgc atggctacca gaaaaaccgg aaggcgaaaa accagtacaa aatactaata
    13261 aagaattaca gttacaagaa ctgaacaaga agtatcagat ggccaaagag gcaattgaaa
    13321 gtgctacaac tcttgacgat gttgaaactc agtttgataa atacacaaaa gttggagata
    13381 aagataaata cccggattct ttacgtaacc aatatactca aggagataaa gataaagaaa
    13441 tagaaaaagc taaaaaatca ctgggtgatt taagcgataa agtaaatgga aaaattgaag
    13501 aagataagtg gctatctgcc gaagttaaaa aaaaacaaca gcaagaatta gaagcgcgta
    13561 aacaaaaagt aaacgactct cttaaaggat ctgatagttt aaaatcattg agagaaacag
    13621 tggaaaaagc ttcttctaaa aatcagaaaa aaccagaatc ttttgaagat gtttatgttc
    13681 ctggaaatga agaaactgaa aaaacaaaag ttcgtgatat acttcaaaaa acttatcaaa
    13741 aaacagagca aaatattgaa acagatccat ggttgagtcc ggaacagaaa aaagctcaaa
    13801 aagaaaacgc taaaacaaga ttagatgcag gtttgaaagc tgtcgaaact acagaaagct
    13861 tagataaact taaagaagtt gagagtgact tccttgataa agaaaaaaat ccagattcaa
    13921 ttccaaatca acataaagct ggaactcctg aagttgctcg caaaactttc ttagataact
    13981 ttgaaaaaga agctaaaaaa gagattgaat caattaaaaa tgacgttaca ttaacagatg
    14041 ctgaaaaagc aacagctaaa gctaaagtag aagcacaact tcaagaagct aaaggtaagg
    14101 ctaaggaatc aaaatcgttt gatgatttga agaacattca agataagttc aattctgaat
    14161 taccacacac accaggtaag ccgcttaaag atcaacaatc tgatgctatt gcagaattag
    14221 agaagaagca gcaagaaatt gaaaaagcta ttgagggtga taaaacatta ccaagagacg
    14281 aaaaagagaa acaaattgct gactctaagg aacgcttaaa atctgacact caaaaagtta
    14341 aagatgctaa aaatgctgat gctattaaaa aagtatttga agaagggaaa gtgaatattc
    14401 ctcaagcaca tatcccaggt gatttgaaca aggataaaga aaaacttctt gcagagttga
    14461 agcaaaaagc agctgatact gaaaaagcga ttgattccga taaaactttg acagcgaaag
    14521 aaaaagaaga gcaaaaagcg aaaacaaaag aagaacttca aaaagcaaca gaagctgttg
    14581 gagcaattga caatcgtgaa gaactagata aaaaagtccc agaacttaag aaagctattg
    14641 aagacactca cgttaaaggt aatcttgaag gtgttaagaa taaggctatt gaagatctta
    14701 aaaaagttca tgacgaaaca gttgctaaga taaatggtga tgataccctt gacaaagcta
    14761 ctaaagaagc tcaagtgaaa gaagctgaca aagctttggc agcaggtaaa gatgcgatca
    14821 ctaaagcaga tgatgctgat aaagtgggtg cagctgttac agagcacaca ccaaaaatta
    14881 aagcagcaca taaaactggt gatcttaaaa aagttcaaga agaggctaac caagctcttg
    14941 acaaagcagc tgaaaaagaa cgtggagaaa tcaataatga tatcacatta acggcaaaag
    15001 acaaagaaca acaactgaaa gaagttgaga cagctcttac taaagctaaa gataacgtga
    15061 aagctgctaa gacagcagac gctatcaatg acgcacgtga taaaggcgta gcaacaattg
    15121 atgccgttca taaagcaggt caagacttag gtgctcgtaa gtcaggtcaa gtcgctaaac
    15181 ttgaagaagc agctaaagca acgaaagaca agatttcagc tgatccaact ttgacaagca
    15241 aagaaaaaga agagcaatct aaagctgtta acgctgaact taagaaagcg atagaagctg
    15301 ttaacgcagc tgacacagct gacaaggttg acgatgctct tggtaaaggt gtcacagaca
    15361 tcaagaacca acataagaca ggtgaccctg tcgtagctcg tcgtgaggct catggtaagc
    15421 aacttgatag agtcgctcaa gaaactaaag atgcgattga aaaagaccct actttgacga
    15481 ctgaagaaaa agctaaacaa gttaaagacg tagatgccgc taaagaaaga ggcatggcta
    15541 agcttactga agctaagaat gctgatgagt tagacaaagc ttacggtgaa ggtgttacag
    15601 acatcaagaa ccaatacaag tcaggtgacc ctatcgaagc tcgtcgtgga ttacataaca
    15661 agtcaatcga caaagtggcg caagcaacta aggacgctat cacagcagat acgactttga
    15721 ctgaagccga aaaagaaaca caacgtggca atgttgataa agaagcaact aaagctaaag
    15781 aagaacttgc taaggctaaa gatgctgatg ctttagacaa agcgtacggt gacggtgtga
    15841 ccagcatcaa gaaccaacac aagtcaggta aaggtcttga cgttcgtaaa gatgagcaca
    15901 agaaagctct tgaagctgtt gctaaacgtg tcactgctga aattgaggct gatccaacct
    15961 taacaccaga agtgagagaa caacaaaaag cagaggttca aaaagagctt gaacttgcga
    16021 ctgataagat tgctgaagct aaagatgcag atgaagcaga caaagcttac ggtgatggtg
    16081 tcacagcgat cgaaaatgcc cacgttattg gtaaaggtat cgaagctcgt aaagaccttg
    16141 ctaagaaaga ccttgctgaa gctgttgcta agacaaaagc tctcattatt gaagacaaaa
    16201 cgcttactga tgatcaacgt aaagaacagt tatcaggtgt tgatacagag tatgctaaag
    16261 gtatcgagaa tattgatgca gctaaggatg ctgcaggtgt tgataaagca tatagtgacg
    16321 gtgttcgtga catcctggca cagtacaaag aaggtcaaaa ccttgatgat cgtcgtaatg
    16381 ctgccaaaga atttcttctt aaagaagcag acaaagtgac gaaactaatc aatgatgatc
    16441 caaccttgac tcatgaccaa aaagttgatc aaatcaacaa agttgaacaa gctaagttag
    16501 acgcaatcaa gtctgttgat gatgctcaaa cagctgatgc tatcaatgat gctcttggta
    16561 agggtattga aaacatcaac aaccaatacc aacatggcga tggcgttgat gttcgtaaag
    16621 cgactgccaa aggcgatctt gaaaaagaag ctgctaaagt gaaagctctt attgctaagg
    16681 atccaacctt gactcaagct gataaagaca aacaaacagc agcggttgac gcagctaaga
    16741 acacagcaat tgcagcggtt gataaagcga caacagcaga tggtgttaac caagaacttg
    16801 gtaaaggcat cacagctatc aacaaagctt accgtccagg tgaaggtgtt aaagcacgta
    16861 aag
//
LOCUS       DAUGHY010000036        15463 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.036, whole genome shotgun sequence.
ACCESSION   DAUGHY010000036 DAUGHY010000000
VERSION     DAUGHY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15463
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.036"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            68..637
                     /locus_tag="VH752_001238"
     CDS             68..637
                     /locus_tag="VH752_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ8289588.1"
                     /translation="MGKEIKVKCFLRRSAFGLVAVSASVLVGSTVSAVDSPIEQPRII
                     PNGGTLTNLLGNAPEKLALRNEERAIDELKKQAIEDKEATTAIEAASSDALEALADQT
                     DALQSEEAEVVQSDNAASDAWEKAATPIALDVKKTKDTKPVVKKEERQNVNTLPTTGE
                     ESNPFFTAAALAIMVSTGVLVVSSKCKEN"
     gene            875..1417
                     /locus_tag="VH752_001239"
     CDS             875..1417
                     /locus_tag="VH752_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8289589.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIEHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            1426..2709
                     /locus_tag="VH752_001240"
     CDS             1426..2709
                     /locus_tag="VH752_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ8289590.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCEDSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMVYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            2725..3585
                     /locus_tag="VH752_001241"
     CDS             2725..3585
                     /locus_tag="VH752_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEQ8289591.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTGSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            3587..4552
                     /locus_tag="VH752_001242"
     CDS             3587..4552
                     /locus_tag="VH752_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEQ8289592.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSTF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSDLSPSQSIEDSHTD"
     gene            4663..5946
                     /gene="aroA"
                     /locus_tag="VH752_001243"
     CDS             4663..5946
                     /gene="aroA"
                     /locus_tag="VH752_001243"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEQ8289593.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSHLKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDTLNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            5939..6430
                     /locus_tag="VH752_001244"
     CDS             5939..6430
                     /locus_tag="VH752_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEQ8289594.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIESQVLKDLLFANDNSVIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            6638..8089
                     /locus_tag="VH752_001245"
     CDS             6638..8089
                     /locus_tag="VH752_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ8289595.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDAPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIL
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILVLGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYMMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKTLAKLGLR"
     gene            8138..9526
                     /gene="rlmD"
                     /locus_tag="VH752_001246"
     CDS             8138..9526
                     /gene="rlmD"
                     /locus_tag="VH752_001246"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ8289596.1"
                     /translation="MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVD
                     NALPEEVIDMRVLKVNKNSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQ
                     LTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKH
                     SHDLIPISDYYIQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGE
                     MMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTI
                     TDSMLGNNYAISAQSFYQVNTIMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFA
                     KTVKAVYGVEVIEAAVRDAQQNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVD
                     PPRKGLTESFIQASVAMGPQKIIYISCNPATMARDIKRYQELGYKLTKVQPVDLFPQT
                     HHVECVVLLIKE"
     gene            9565..10359
                     /locus_tag="VH752_001247"
                     /pseudo
     CDS             9565..10359
                     /locus_tag="VH752_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            10444..10845
                     /locus_tag="VH752_001248"
     CDS             10444..10845
                     /locus_tag="VH752_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEQ8289597.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     NAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKISLTMFVTGRRHNG"
     gene            complement(10950..12434)
                     /locus_tag="VH752_001249"
     CDS             complement(10950..12434)
                     /locus_tag="VH752_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEQ8289598.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLEKNGIASYLHYPIKGYPTDINHIISSEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIEASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQYHKLYRK"
     gene            12808..14028
                     /locus_tag="VH752_001250"
     CDS             12808..14028
                     /locus_tag="VH752_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ8289599.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLGL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGIAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     LSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPIKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLCFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISFGAMW
                     TIGQLKAERYQQLR"
     gene            complement(14148..14513)
                     /locus_tag="VH752_001251"
     CDS             complement(14148..14513)
                     /locus_tag="VH752_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEQ8289600.1"
                     /translation="MTIYSHTYETRPNHSAKAMGSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVGEQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            14627..15358
                     /locus_tag="VH752_001252"
     CDS             14627..15358
                     /locus_tag="VH752_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ8289601.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKAVFPVGRL
                     DKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
BASE COUNT         4807 a         2687 c         3278 g         4691 t
ORIGIN      
        1 aattataatt tccaaattat cggtaattta atatgctaat gcatataaaa ataaaaaagg
       61 agaaacaatg ggaaaagaaa taaaagtgaa atgctttttg cgtagatcag cttttggatt
      121 agttgcggtg tcagcatcag tattagtcgg ttcaacagta tctgctgttg actcacctat
      181 cgaacagcct cgaattattc caaatggcgg aaccttaact aatcttcttg gcaatgctcc
      241 agaaaaactg gcattacgta atgaagaaag agccattgat gaattaaaaa aacaagctat
      301 tgaggataaa gaagctacga cagctataga agcagcaagt tcagatgcct tagaagcatt
      361 ggcggatcaa acagacgctt tacaatcaga agaagctgaa gtagttcaat cagataacgc
      421 tgctagtgac gcctgggaaa aagcagcaac tccaatcgct ttagatgtta agaaaactaa
      481 agatacaaaa cctgtagtta aaaaagaaga aagacaaaac gttaataccc ttcctacaac
      541 tggtgaagag tctaacccat tctttacagc tgctgcgctt gcaataatgg taagtacagg
      601 tgtgttagtt gtaagttcaa agtgcaaaga aaattagttg ctatttgttc tagcaaatga
      661 aacaagggaa tcgaaagatt ctctttttct gatatttaaa aatgctacag tttataatag
      721 cttgaaagac taaatgttct caaactcatt agaacaagct ttaataaaac taacccagtt
      781 gtatcaaagg atagataaag actaagactg ttatagaata tttgcaaaag atttgttata
      841 atggacccaa tacaggtatt gtgaaaggta taatatggat atttggacaa agcttgcagt
      901 gtttgctttt tttgagactc cgaaagtaat attacggcct tttcgctatg aagatcattg
      961 ggatttttac agcatggtta acgacactaa aaacctttat tatgtttttc cagaacaaaa
     1021 aactaaggca gcaagtgact atcttttagt acatagtttt ataaagtttc ctttaggtca
     1081 gtgggcaata gaagataaag caacccacca agtaataggt tctattagaa ttgagcatta
     1141 tgatgctaaa acgcgttgtg ctgatattgg ctatttttta aactatgcct tttggggaca
     1201 aggaattatg acagaagtcg taataaaact tgtttattta tcttttcacg aatttggtct
     1261 caaaacgttg cgtattataa ctcatttaga aaataaggcc agccagaaag tagctaaaaa
     1321 agcaggtttt caactaaaaa cctgttttaa aggaagtgat cgtaatactc ataaaatctg
     1381 catttataaa atgtaccaac taactaatga taggtgataa tcattatgag caaacatcaa
     1441 gatattttag attatttaga aaaacttgct attggtaaaa aagtgagtgt tagaagcatt
     1501 tcaaaccact taaaagtaag cgatggaaca gcctaccgtg ctattaaaga ggcagaaaat
     1561 cgaggtattg tcgaaaccaa accaagaagt ggaactgttc gtattgaaaa aaaaggacgg
     1621 gttcgtattg accgtctgac ctattccgaa attgctcgga tcagtgattc ggaagtgcta
     1681 gcgggacatg ctggtttagg acatgaattt agtaggtttt caattggtgc tatgactcaa
     1741 caaaatattc gtcgttactt ggtcaaagga gggttattaa tagttgggga tcgtgaaacg
     1801 attcaactct tagctcttga aaaccataat gccattttgg tgacgggcgg atttccagta
     1861 tccaaacgtg ttattgaaat ggcaaataac caacgaatcc cagttatggt aactcattat
     1921 gataccttta ctgtggcaac aatgattaat cacgcgctat ctaatattcg tattaaaaca
     1981 gatctcaaaa cagttgaaca ggtaatgata cctattacag attatggtta cctttgcgaa
     2041 gacagctctg ttgaagaatt caatacatta ataaaaaaaa cacgacaagt caggtttcct
     2101 gtattagatt ataaaagaaa agtgattggt gtagttagta tgcgtgatgt ggtggatcag
     2161 ttgccaacaa ctaagcttac caaggtcatg tctaaaaatc caataacggc tagaccaaat
     2221 actagtcttg ctaatattag ccagaaaatg atctttgaag atttaaacat gttacctgtc
     2281 actgacgaag aaaataattt acttggtatg attactcgta gacaagcaat ggaaaatttg
     2341 ccaaatcacc aaccaaataa cccctatact tatagtgagc aaatactttc taaccttgaa
     2401 gaaacggttg attattacca agtagtagtt gagccaacaa tgattgatag tgctggtaac
     2461 atgtcaaacg gtgttatttc agagttctta aaggaaatta gtatcagagc cctcacgaaa
     2521 aagcaccaaa aaaatatcat tatcgaacaa atgatggtat actttttaca tgctattcaa
     2581 attgaggatg aattaaaaat atatcctaaa atcattactg aaaatagacg aagcagcacc
     2641 attgatattg aaatttttgt ggatgatcaa gtgattgcta aagctattat tacaacgaaa
     2701 ataaattaga ggagtaacaa aacaatgata acattaaaat cagcccgtga aatagaagcg
     2761 atggatagag caggagactt cttagcaggt atccatattg gcttaaggga tattattaag
     2821 cctggtgtgg atatgtggga agtggaagca tatgttcgtc gccgttgtaa agaagacaac
     2881 gttcttcctt tgcaaatagg ggttgatggt cacatgatgg actatcctta tgcgacctgt
     2941 tgtggtctaa atgacgaagt agcacatgcc tttccccgtc attacattct gaaagaaggg
     3001 gatttgctta aagttgacat ggtactcagt gagcctttgg ataaatcaat tgttgatgta
     3061 gcagctttag attttgataa cgtgcctgag atgaagaaat ggacaggatc ttataccggt
     3121 ggtttagctg actcttgttg ggcttatgct gttggaacac cgtctgacga aataaagcaa
     3181 ttaatggatg tcaccaaaga agccatgtac cgtggtattg aaaaagcagt aattggcaat
     3241 cgtattggtg atattggcgc agctgttcaa gaatacgctg aaagcttcgg ttatggtgtc
     3301 gtgcgtgatc ttgttggtca cggtgttggt cctacaatgc atgaagagcc aatggtacca
     3361 aactatggaa cagcaggtcg tggacttcgt ctaaaagaag gcatggtctt gactgttgag
     3421 ccaatgatta atacaggaac ttgggaaatt gataccgaca tcaagacagg ttgggctcat
     3481 aaaaccctag atggtggttt atcttgccaa tacgaacacc aatttgtgat tactaaagat
     3541 ggtcccgtta ttttaacaag tcaaggagaa gaaagaactt actaaaatgg cagaaaaaaa
     3601 atggtttgat aaggtactgt ccaagtggca gtatgaaccc attcaagtct ttatgaggca
     3661 tttacaaagt gcagaaatgg atttatctgc cattgcggtt gcgtattacc ttatcttaac
     3721 ggcctttcca ttaattgtta ttgcagccaa tatttttcct tacctcaata ttgatattgc
     3781 tgatttgtta cgtttaatga agcaaaattt gcctaaagat atttttagac cggcttctgc
     3841 aattgtagaa aatattttct ctaaaccgtc tggaagtgtt ttgggagtag cgactttgac
     3901 aggattatgg acaatgtctc gaagtttgac atcgctgcaa aaagctatta ataaagccta
     3961 tggcgcttct cagcatcgag atttctttat cggacacttg gttggtttgt tgaccagttt
     4021 aattatttta tttttattag cctttgcctt aatcttttca acattttcta aagctgctat
     4081 tcaagtgctg gataagcatt accatttgag tgataatatc acaacgattt tcttgctcct
     4141 cattcaacct attacagttt tgattatttt tgttggcttg atgctactat attttttatt
     4201 acccaatgtt aagattaaaa aaattcgcta cattttacca ggaaccctat ttacatcttt
     4261 tgtgatgaca tttttgagta atctggttgg caattacgtt gtgtataatg ttgagcggat
     4321 ggtagatatt aaaatgtttg gttcagttat gatttttatc atcatgctat ggtttatttt
     4381 cttagcaaga atcctgattt taggagctat atttaatgcg acctatcaag aaatgtcctt
     4441 ggggaaatta gaaggtcgta gtggtgatat gatcgctatc cttaaaaaaa cacttgggaa
     4501 tgattcagat ctatctcctt cacagtctat cgaagatagt cacactgatt gatacaaaaa
     4561 agataataag tcgtggagtg agaaactatt ttcttgcttc attttttatg aggatgaata
     4621 gaaaatcatt gatcttagca aatagaaagg cagatgaaac ggatgaaatt gcgaacaaat
     4681 gcaggaccat tacagggaac gattcaagtt ccaggagaca agtccattag tcaccgggca
     4741 gtcatattag gagccgttgc taaaggggaa acacgagtaa aaggactctt aaaaggagaa
     4801 gacgtgcttt ccacgattca agcctttagg aatttggggg tccgtatcga agaaaaagat
     4861 gaccagcttg tgatagaagg tcaaggtttt caaggattga ccgctccttg tcagacactt
     4921 aatatgggaa attctggaac ctctatgcgc ctcattgctg gattattggc aggtcaacct
     4981 tttagtgtca aaatgattgg cgatgaaagc ttatccaaac gtcctatgga tcggattgtt
     5041 tatccgttaa agcaaatggg ggttgagata tctggagaaa cagataggca atttcctcca
     5101 ctacaattgc aaggtaatcg taatttacaa ccaatcacat ataccttgcc tatctcttct
     5161 gctcaggtta agtcagctat tttattagca gctttgcaag caaaaggaac aacacaggtt
     5221 gttgaaaaag agattactcg aaaccataca gaagaaatga ttcaacaatt tggtggacga
     5281 ttgatagttg atggtaaacg cattactttg gtgggacccc agcagctcac tgctcaagag
     5341 ataactgtcc ccggagatat ttctagtgca gctttttggc ttgtcgccgg cttaattatt
     5401 cctggctcgg agctcctttt aaagaatgtt ggagtcaatc cgacacgaac agggattctt
     5461 gaagtggttg aaaaaatggg agcacagatt gtctatgaag atatgaataa gaaagagcaa
     5521 gtaactagta ttcgagtggt atattctcac ttgaaaggaa ctatcatatc aggaggtctt
     5581 atccctcgat tgattgatga acttcccatc attgcacttc ttgcaaccca agcacaagga
     5641 acaacctgta ttaaagatgc tcaagaatta cgagtcaaag aaacagatcg tattcaagta
     5701 gtaactgata ctcttaacag tatgggagct aatatcaaag cgacagcaga tggcatgatt
     5761 atcaaaggcc ctacggtact atatggggct aacacaagta cctatggaga tcatcgtatt
     5821 ggtatgatga cagcaattgc agccttgctt gttaaacaag gacaggtcca tttggataaa
     5881 gaagaagcca tcatgacgtc ttatccgacg tttttcaaag atttagaaag gctgtgtcat
     5941 gactaaagtg ttattgggat ttatgggagt ggggaaaaca acggtttcga aacacctaag
     6001 tatgcactgt aaagatatgg atgctattat tgaagctaaa atcggtatgt ctattgcagc
     6061 tttttttgaa cagcatggag aaatagcatt tcgaacaatt gaaagtcaag tgttaaaaga
     6121 tctattgttt gcaaatgata acagtgtcat cgtaacaggt ggcggtgtag tggttttaca
     6181 agaaaatcga caattactga gaaaaaatca ccagcataat attttattgg tagcttcttt
     6241 tgaaactttg taccagcgtt taaaacatga taaaaagtct caaagacctc tatttcttaa
     6301 gtactctaag gaagcatttt atgaatttta tcaacaacgt atggtatttt atgagggact
     6361 ttcagattta gtaatcagag ttgaccatcg tacgcctgaa gaagttgcca acatcattga
     6421 gggttattag caggtcattg tcgtaatctg atatttatgg ttttaaacag gtatctgtct
     6481 aaagcacttc ttatggcaag ttggctttat taaatattag gaaataatct tgattattct
     6541 ataatgatta aaattttgag aggacaatct ggctaatata ggttataatg gtagtattcg
     6601 ttaaaagtgc aattgtaata ctaagagatg ataagatatg acaaaatacc ccatgggtgg
     6661 ccttagtcac catgaagaat tacgttattt ttatctccta aggaatttga gttaccttag
     6721 tgagaatgaa aaaaaagagt ttgctttttt aaaatctaaa ttggaaattg gtcgagctta
     6781 tgcgccttct aagcagcatt ataggaagtc aaaacggcaa gaaccttact ttgaagatga
     6841 ctactataac gactactcac caaatgattt actagaagat gatgacgtca atcatgactc
     6901 ttcttttgtt ccatatggtt taccgatata tcctaaagaa gacagatatt taaacaaaaa
     6961 aacaaaactt acagctcgta gacctataga tgctccgcag cctattgatg aagacgatgc
     7021 ttttttaaca gagtctgttg ctcgttgtgc tcttcctaga tctcaaaaaa gaaagcataa
     7081 gaaaaaagga tgtatgaaat ggttttttaa cattcttggc ttattgctaa tgactgtgct
     7141 tatggggtta gggttgatgt ttgcaaaagg ggtttttgat atttctacta ataaagctaa
     7201 ttacaaacca gctgtttccc aagcttttga tggtcaagaa actcaagatg gtacgaacat
     7261 tctggttttg ggttctgacc aacgagttac tcaaggttct acagatgctc gcacagatac
     7321 cattatggtt gtcaatgtcg gcaaccatgc taaaaaaata aaaatggtct cttttatgcg
     7381 tgacactttg attaacattc caggatacag ttataatgat aattcgtatg atcttaaatt
     7441 aaatagtgca tttaatcttg gagagcaaga agatcaccac ggagcagagt acgttcgacg
     7501 ggccttaaaa cataattttg atatcgacat taagtattat atgatggtag attttgaaac
     7561 atttgcagaa gctattgaca ccttgtttcc aaatggtgtt aagattgatg ccaaatttgc
     7621 gacagtaggt ggtgtagctg ttgactccgt agaagttcca gatgacctgc gaatgaaaaa
     7681 tggggttgtt cccaaccaaa ccatcgaagt tggagaacaa cgcatggatg gtagaacctt
     7741 gctcaactat gctcgtttcc gtaaagatga cgaaggagat ttcggacgaa ccgtccgaca
     7801 gcaacaggtt atgtcagctg ttatgtccca aatcaaggac ccaactaaac tctttacagg
     7861 gtctgcggct attggtaaaa tctatgcgct gacttcgacc aatgtttctt ttccatttgt
     7921 tgttaaaaat ggtgtttctg ttttaggaag tgggaaaaat ggagttgagc acgtgaccat
     7981 cccagaaaat ggggattggg ttgacgaata cgatatgtat ggaggacaag ccctttacat
     8041 agattttgat aagtatcaga aaacgttagc caaattaggt ctgcgctaag atatggtata
     8101 atggagagta actaaagttg ctcttctttt ttgagcaatg gttagcccta gaaaaggaaa
     8161 aagaataaga atgttgaaaa aaaatgatat tatccaagtt gctatttcag atttaagcca
     8221 tgagggagca ggtgtcgcta aacacgatgg ttttgttttc tttgttgata atgcattgcc
     8281 agaagaagtg attgacatgc gtgtcttaaa ggtcaataaa aatagcggtt ttggcaaagt
     8341 agaagcctat cattacctgt cgccagctag aaatgcagat gtcaatttaa cttatttgcg
     8401 aacaggcatt gcagaccttg gacatctaac ttatgaagac caactgactt tcaaaaagaa
     8461 acaagttcag gacagcctct ataaaattgc tggaatcagt gacgtgacag tcgaaagcac
     8521 gattgggatg acagaacctt tagcttaccg aaataaggct caagttcctg ttagacgtgt
     8581 taacggtcag ctagaaacag gctttttccg caagcattct catgacttga ttcctatttc
     8641 ggattattat attcaggaca aagagattga tcgtctcatt aattttaccc gtgatttatt
     8701 acgccgcttt gatattaaac cgtatgacga aacagaacaa acaggtctct tgagaaatat
     8761 tgttgttcgc cgaggccatt actctggaga aatgatgctg gtcttagtga ctacacgccc
     8821 aaaagttttc cgtgttgacc aagtcattga aaaaatcgtt gaagcttttc cagcagtggt
     8881 gtctattatt caaaatatta atgataagaa caccaatgca atctttggta aagatttcaa
     8941 aacgctttac ggcaaggata ccattactga tagcatgctt ggaaacaact atgccatttc
     9001 tgctcagtct ttctaccaag tgaatacgat tatggcagaa aaactctatc aaacagctat
     9061 agccttttca gacctttcta aagatgacat tgtgattgac gcctactcag gtatcggaac
     9121 cattggccta tcatttgcta aaacagttaa agctgtttat ggtgttgaag tgattgaagc
     9181 agctgttaga gatgctcaac agaatgccgc tcttaatggc atcaccaatg cctactttgt
     9241 cgcagatacc gcagaacatg ccatggcaac atgggcgaaa gatggcatta aaccaagtgt
     9301 tattctggta gacccgccac gaaaaggctt aacagaaagt ttcatccaag ccagcgtggc
     9361 aatgggacca caaaaaatca tctacatttc ctgcaaccca gccaccatgg cgcgtgacat
     9421 caagcgttat caagagttgg gttacaaact gaccaaagtg cagcccgtag atttgtttcc
     9481 gcaaacgcat cacgtcgagt gtgtagtgtt gctaatcaag gagtaaggta gctgcctcca
     9541 catgacaaaa ataaggagaa acgaatgcct attacccaac acgagattcc ccttttagaa
     9601 tgtgatagta atccatcagc cgtcgttata cctacacatg aaggtctagc tatttagctt
     9661 ccagctaagt gtgtctatgc tttcttagaa gacgagattg aagcttttgc tttagaccat
     9721 caaggtaaaa aaagtagcag aattcatttc agccacaaag gtttaccctg tttatcagat
     9781 atcttataaa ggtcaagaca tttgcctagc tcaagctccc gttggctcag ctcctgcggc
     9841 gcaattcatg gactggctga ttggttatga ggttaaagaa atgatcagta caggaacctg
     9901 tggcgttttg gttcctatcg cagaaaaccg ttttttagtg ccagttaagg ctttgcgaga
     9961 tgaaggaaca agttatcatt acgtggcacc atctcgttat attgatattg atcctaagat
    10021 gcttagactg attgagaaaa gcttttagca caggggttag cttatcaaga ggtcatcacc
    10081 tggtcaacag atggttttta ccgagaaacc aaagaaaaag tggcttatcg ccaggaagaa
    10141 gggtgttcgg ttgttgagat ggaatgttct gccttagcag cagtagctca attaagaggc
    10201 atcctttggg gacaactctt atttacagca gatactctag cagatgtaga ggtttacgat
    10261 caacgcaact ggggcgctga ttcctttagt tttgcgctcc atttatgctt ggaagtactc
    10321 aatacgctag aaaaggatgg aaaggctact gatttttgaa acatgagctc tgccttctgt
    10381 tcctatttgt agtataatga ggcttgtatt gcttttagac taagcttaga aaagagaaaa
    10441 acaatgtcag atttaacaca agagatgacc ttaaatgtta ttagtatttt tgataattac
    10501 caaattgaac ttgctgaaaa aggacacctt attttaagta ctgaagtgac tgagacggct
    10561 ttgaattatt atgggaatgc ccatggtggt tatcttttca ccctatgtga tcaagtaggt
    10621 ggtttggttg ctagaactac tggcgtagaa tctgtcacct tacaggccaa tgctaactat
    10681 ttaaaagcag ggcacaaggg agacaagctg atggttgaag gtcgattggt tcatggaggt
    10741 cgaaccacgc aggtggttga tgtgacgatt cataaccaaa caggagctct tctaacaaaa
    10801 ataagcttga ccatgtttgt gactggccga cgtcataacg ggtgattaat gaatcaataa
    10861 aggaaaaaag tcccaactct ctgagaattg ggactttttt cctttattga gagataccat
    10921 aaatcacccg ttagacgggt ggttatggtt tatttacggt aaagtttgtg atattggtaa
    10981 acagggtcaa aggtaatatt taccccaagt tttcggagta catttttatc ttcttcggtt
    11041 aagataacgg tagaatgggc ttcgctacct ttcaaattgc caagttcttt catggctaga
    11101 ttagcttctt cactagtcat agcagtaatg gccaacgcaa tcagaatttc attggaatga
    11161 agccgcggat tgtgactgcc taagtggttc actttgaggc cttgaatagg tttcacgtat
    11221 tcgggttcaa tcaaatgagt ggttttatca atctttgcca aggttttaat cgcattgatg
    11281 ataacagctg cagttggtcc aaagagttca gatgttttac ctgtcacaat ctgcccattt
    11341 ggtagttgga gagcaagggc aggttggcct gtttgctctg ctttttgatg agcagctaca
    11401 gtaacgtgac gatcgtcagg ggtgacaccg atatcattca tcaagagctc aatttttttg
    11461 acagcagatt ctgtgactcg ctctgcctta aaatccacga gggtttggta ataacgacgg
    11521 atgatttctt gtttagaagc ttcgatagca gcttcttcat tgacgataga aaaaccaacc
    11581 atgttaacac ccatatcggt tggtgaggca taaggagact tgctcaaaat acgctcgaag
    11641 gtccgattta agacaggaaa gacctcgata tcacggttgt aattaacggc cgttttgcca
    11701 taagtttcca aatggaaggg gtcaatcata ttcacatcat ctaggtctgc cgtggctgcc
    11761 tcataagcca gattgacagg gtgatgaaga ggaagattcc aaactgggaa ggtttcaaat
    11821 ttagcatagc cagacttaac cccattgatt tggtcgtgat acatttggga catgcaagtg
    11881 gctaattttc cagacccagg gcctggtgct gtaacaacga taaggttacg actcgttttg
    11941 atgtaatcat ttttccccat cccctcagat gagataatat gattaatatc tgtcgggtag
    12001 cccttgatag gataatgcag gtaagaagca atgccatttt tttcgagttg cttgcgaaag
    12061 gcatctgcag cgggttggtt attgtattga gtaataacga cagaacctac gtaaatatct
    12121 aacgtgttaa atttgtcaat taagcgaaag acttcttggt cataagaaat gcctaaatca
    12181 ccacgcgctt tagaatgttc aatattatta gcgttaatgg caattacaat ttcgacttgt
    12241 tctttgagct ctttgagtaa tttaatctta ttgtcaggtt catagccagg aagcacacgg
    12301 gcagcatggt aatcttctaa cattttacca ccaaattcca tgtaaagctt gccatcaaat
    12361 tggctgatac gttctaaaat atgatctctt tgcaagttta aatacttgtt agaatcaaaa
    12421 gcaatagtct tcattttttc acctctgtaa agcattataa caaagaaatt ccaagaaaga
    12481 aaaggttttt tgcagggata cctcattttg tgacttttta aaaaaactta ggcttggttc
    12541 ttagaagaaa aatgtatccg ctatgttcaa aagaacttga caattaaaat gagctttact
    12601 atactagtaa caattaaata ctattttaaa catgttccgt gaagcatgaa agtcttaaca
    12661 gtaaagagag tttttttgtc atctcttatt atttagtgaa ctttgatgga cttgcggact
    12721 tgtttccaag tctttttttg acctttggac aaagggtatt caattaatta aggtagtcag
    12781 tgagctacta ttaagaagga gacaattatg tcaacattat cattggacac cacaaataag
    12841 cgagctttag tggcagctat tgcagcttca ggaacggatg acttgaatgt catgtttctt
    12901 gccttttcca tgtcctccat tatgacagac ttggggctta gtggcactca gggtggctgg
    12961 attgcgacca ttaccaattt aggaatgttg gtgggaggcc tcttattcgg gttgctggct
    13021 gataggcacc acaagtttaa ggtctttaaa tggacgattt tactgttttc agtagcaaca
    13081 ggattaattt attttaccca gtctttgccc tacttgtact tgatgcgatt tattgctggc
    13141 ataggagttg gaggtgagta cggtatagcc attgctataa tggcagggat tgtaccacca
    13201 gaaaaaatgg gtcggatgtc ttctttaaat ggtattgctg gccagcttgg ctccattagt
    13261 tcggctcttt tagcagggtg gctggctcta agtctaggct ggcgtggcct ttttctcttt
    13321 ggattacttc cgattctctt ggtgatttgg atgactttag ccatagatga ccaaaaaatt
    13381 tgggatcatt atgggcaaga ggaggaagaa tgcagtcaac caattaaaat caatgaacta
    13441 ttcaaaacaa aatccttaac agctcaaacc cttgctttaa tggtcatgac gactgtgcag
    13501 attgcgggct attttggaat gatgaattgg ttgccaacca ttattcaaac aagcttaaac
    13561 ctttcagtaa aaagttcgtc cttgtggatg gtggctacta ttgtcggcat gtgtttgggg
    13621 atgctgtgtt ttggtcagct tttagattgc tttggtccac gtctgattta ttccctcttt
    13681 ttattagcat catctatttg tgtttacctt tttcaatttg ccaattccat ggcgagtatg
    13741 gttattggag gtgctattgt cggttttttt gtcaatggga tgtttgctgg ctacggggct
    13801 atgattacca gactttatcc ccatcacatt cgatccacgg ctaataatgt tattttgaat
    13861 gttggccgtg ctctaggagg attttcgtcc gttgctattg gtagtatttt agatgcctca
    13921 ggtatttcaa tggtcatgat tttcctagct tctctgtatg tgattagttt tggagccatg
    13981 tggactattg gtcagttaaa agcagaacgc taccaacaat tgcgttagca aagagatggg
    14041 tgttagtatt tgagttactt tcactcacaa aactatttta ttgattgaga taagcaaaaa
    14101 ggtaagcgct tagggattgc ttaccttttt ggttcttttc ttttgtttta ctccgctact
    14161 ttggccataa aatgtttact gttcacccta actctagtat gacaggcttt tccaatgagt
    14221 tgttctccca caaaagcttc caagcggaaa tcgtattttc ggccttcttc tttaagggct
    14281 gtaatgatga ttgtaacagg atctccaatg gctgaagctg ccaaatgttg gatagctatc
    14341 tcgctgccaa ccgttgtaaa gtcactgtcg agctgttctt gggcaaataa gtaagaagca
    14401 ttttccataa aagcaacaag agcaggagta gctaggacat tcagcgttcc tgagcccatg
    14461 gctttagccg aatgattggg acgggtttca taagtgtgag aataaatagt cataagttaa
    14521 tctcctcgtt tctataagcc tattataata tattaagcgc ttacagtcat gaagccactg
    14581 ttttttatgt tatacttaat aatgaatttt ctttgaaagg gttatgatgc gcttagataa
    14641 atttttagtg gcaactggtg ttggaactag gagccaagtc aaacttctct taaaaaagaa
    14701 ggctattttc gtcaatcaaa aggttgaaac ctctgccaaa gcacacattg atgaatacaa
    14761 ggatttagtg acgtatcaag gcacgccctt ggtctatgaa tcctttgttt attacctgtt
    14821 aaacaagccg tcagggtatg tgtcagcaac ccaggacaga cagcaggcta ctgttatgga
    14881 gttgttagat gacacggcta ggcaaaaagc agtttttcct gtgggtaggt tagataagga
    14941 tacccgtgga cttttgctct tgaccaataa tggacaacta gcccatgacc ttttatcccc
    15001 taaaaaacat gttacgaaag agtacttggc taaagtagca ggcatcatga ctgaagctga
    15061 taaagattat tttgctagag gtatttcctt aaaggatcac cagtgcctac ctgctcattt
    15121 agaagtgctt gctagtgatt tacagcagca aacaagtttg gttaaaatta ccatacaaga
    15181 aggaaaattc catcaggtga aacgcatggt agcagcctgt ggaaaggaag tgcttgactt
    15241 acagcgtctc agcatgggac cattaaaact agaccctagc cttgcagaag gggagtttag
    15301 gcgcctgacg cctgaggaat tgcaaagcct ggcaccttat tgtcagagct cactataaaa
    15361 agaaacctcc cctgtcagta ctgaccccaa aaagttggac aaataattat tgaaaggatt
    15421 tagttctgta ttggacagga ctaagtcctt ttagttttgc ttt
//
LOCUS       DAUGHY010000037        14883 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.037, whole genome shotgun sequence.
ACCESSION   DAUGHY010000037 DAUGHY010000000
VERSION     DAUGHY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14883
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.037"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            23..727
                     /locus_tag="VH752_001253"
     CDS             23..727
                     /locus_tag="VH752_001253"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEQ8289602.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNKAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVIAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     LKGWFAQIGLNLIDFKLEFGFDQEGTIILADEFSPDNCRLWDKNGNHMDKDVFRRDLG
                     NLTDVYQVVLEKLIAL"
     gene            851..4576
                     /locus_tag="VH752_001254"
     CDS             851..4576
                     /locus_tag="VH752_001254"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEQ8289603.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLTSLKALRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIESLPGQFDQRAVSSQE
                     ALLLFGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPNLDFFETYKADDFTAYKVEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQTTYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRAGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNDETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEARITSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNAYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLV
                     AFGVTTADSRKILSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFEAIQTQHKIT
                     AASAVKYGGVLESFALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADAVKIG
                     QTQADFTVTVNGNDLAGASLLAAFEGKLEEVYPTEFEQTDVLEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEVAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQTLFASAVKYFTGK"
     gene            4737..6191
                     /locus_tag="VH752_001255"
     CDS             4737..6191
                     /locus_tag="VH752_001255"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEQ8289604.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDRGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTVRKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            6219..7241
                     /locus_tag="VH752_001256"
     CDS             6219..7241
                     /locus_tag="VH752_001256"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEQ8289605.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVREPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNNPVKFEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVRAALPLIKEELVNGIGHITGGGFIENIPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            7409..7963
                     /gene="purN"
                     /locus_tag="VH752_001257"
     CDS             7409..7963
                     /gene="purN"
                     /locus_tag="VH752_001257"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEQ8289606.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLTGYMKIVGETLLLAYEGRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            8147..9694
                     /gene="purH"
                     /locus_tag="VH752_001258"
     CDS             8147..9694
                     /gene="purH"
                     /locus_tag="VH752_001258"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEQ8289607.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVVLKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAKKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQDSIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(9752..10876)
                     /locus_tag="VH752_001259"
     CDS             complement(9752..10876)
                     /locus_tag="VH752_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEQ8289608.1"
                     /translation="MKKFHRFLVSGVILLGFNGLVPTMPSTLISQQENLVHAAVLGDN
                     YPSKWKKGNGIDSWNMYIRQCTSFAAFRLSSANGFQLPKGYGNACTWGHIAKNQGYPV
                     NKTPSIGAIAWFDKNAYQSNAAYGHVAWVADIRGDTVTIEEYNYNAGQGPERYHKRQI
                     PKSQVSGYIHFKDLSSQTSHSYPRQLKHISQASFDPSGTYHFTTRLPVKGQTSIDSPD
                     LAYYEAGQSVYYDKVVTAGGYTWLSYLSFSGNRRYIPIKEPAQSVVQNDNTKPSIKVG
                     DTVTFPGVFRVDQLVNNLIVNKELAGGDPTPLNWIDPTPLDETDNQGKVLGDQILRVG
                     EYFTVTGSYKVLKIDQPSNGIYVQIGSRGTWVNADKANKL"
     gene            11129..12394
                     /gene="purD"
                     /locus_tag="VH752_001260"
     CDS             11129..12394
                     /gene="purD"
                     /locus_tag="VH752_001260"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEQ8289609.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLAVGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPVLEGMIAEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGVKFAENSELLLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDPTGLFYRNDIGSKAIRE"
     gene            12672..13163
                     /gene="purE"
                     /locus_tag="VH752_001261"
     CDS             12672..13163
                     /gene="purE"
                     /locus_tag="VH752_001261"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEQ8289610.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            13150..14223
                     /gene="purK"
                     /locus_tag="VH752_001262"
     CDS             13150..14223
                     /gene="purK"
                     /locus_tag="VH752_001262"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEQ8289611.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPANDCPAARV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IVEKDFLTNKAGVTVAPYKVVTSSLDLEGLDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGQDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVTENPSAHLHMYGKLE
                     AKHNRKMGHVTVFAEDADEVEEF"
     gene            14244..>14883
                     /locus_tag="VH752_001263"
     CDS             14244..>14883
                     /locus_tag="VH752_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289612.1"
                     /translation="MELREFKAWLLKNHFEEDELYKESIKCYQVEAYKAAYIFSYLAN
                     HKFIAKLAIDYSGVPSNFMSKCPDDEVRKSSWQKRVNSLKNEDEWEQTVNEKLIQAWE
                     GDNVFKLPFKIKEKYSHMRVLRNNAAHAKDCRISESTVLELWNEIEYIYPYFVINGTI
                     DAWMEDLEKLVRYSNSSIESQNQLQNKFGDFSTENKTLIVKKIIQKYLVDSEW"
BASE COUNT         4269 a         2907 c         3500 g         4207 t
ORIGIN      
        1 agaaagaaag gcgaataatc aagtgactaa ccagcttatt tataaaggaa aagcgaaaga
       61 tatttatagt acaaaagatg aaaatgtgat tagaacggtc tataaagacc aagctactat
      121 gttgaatgga gctcgtaagg agactattga tggcaaggga gcactaaata atcagatttc
      181 atctcttatt tttgagaaat taaataaggc tggtgtggtg acacattata ttgagcaaat
      241 ttcaaaaaac gaacaattga ataaaaaagt tgatattatt ccacttgagg tggtgttacg
      301 gaatgtgata gcaggctcat tttcaaaacg ttttggagtg gaagaaggac gtgttcttga
      361 aacgccaatt gtggaatttt actacaaaaa tgatgatctg aatgatcctt ttatcaatga
      421 tgagcatgtg aagtttttgg gtattgttaa tgatgaggag attgcttatc tgaaaggaga
      481 gactcgccgt attaatgaac tcttgaaagg ttggtttgct cagattgggc ttaacttgat
      541 tgacttcaag ttggagttcg gatttgatca agaaggtaca attatcttag cagatgaatt
      601 ttcgcctgat aactgtcgcc tatgggataa gaatggtaac catatggaca aggatgtttt
      661 tagaagagac cttggcaacc tgacggatgt ctatcaagtt gtacttgaga agttgatagc
      721 attgtaacta gaagaggaag gaattgaatc cgttcgggaa actccgaaaa aaagatggaa
      781 cttactgaaa tctcaatttc agatgtcctc ctattttcca gtcgctccct tgtcggactt
      841 ggtatcttat atgaataaac gtatttttgt tgagaaaaag gctgactttg gtattaaatc
      901 ggctagcctt gtgaaagagt tgacgcataa tctacaactg acttctttga aggctttgcg
      961 tattgtgcag gtctatgatg tcttcaattt ggctgaggat ttgctggcgc gtgctgagaa
     1021 gcatattttc tctgagcagg tgacagactg tcttttgacg gaaactgaaa tcactgcgga
     1081 gcttgataag gttgccttct ttgccattga gtcgcttcct ggtcaatttg accaacgtgc
     1141 tgttagttcg caagaagctt tgctattatt tggaagtgac agtcaggtta aggtcaatac
     1201 agcccagctt tacttggtca ataaggatat tgcagaagca gagcttgaag ccgttaagaa
     1261 ctatcttttg aaccctgttg attcgcgttt caaggacatt actttgccgc ttgaagagca
     1321 ggctttctct gtgtctgata agacgatccc taatcttgat ttcttcgaga cttataaggc
     1381 tgacgatttt acggcttata aggtagagca gggcttggct atggaggtcg atgatcttct
     1441 cttcatccaa gattatttca aatcaatcgg gcgtgtgcca actgagactg agttgaaagt
     1501 tttggatacg tattggtcag accactgtcg tcacacaacc tttgaaactg aattgaagaa
     1561 cattgatttt tcagcttcta aattccaaaa acaattgcag acaacttatg acaaatatat
     1621 cgccatgcgt gatgagcttg gtcgttctga aaagccacaa acacttatgg atatggcgac
     1681 tatttttggt cgttatgagc gtgccaacgg tcgtctggac gatatggaag tctcagatga
     1741 aatcaatgcc tgctcagttg agattgaagt agatgttgat ggtgtgaaag agccttggct
     1801 cctcatgttt aagaatgaga cacacaatca cccaacagaa attgagccat tcggtggagc
     1861 ggcgacttgt atcggtggtg ctattcgtga cccattgtca ggacgttcat acgtttatca
     1921 ggctatgcgt atttcaggcg caggcgatat cacgactccg attgcggaaa cacgtgctgg
     1981 taaattgcca caacaggtta tttctaagac tgcggcgcac ggctattctt catatggtaa
     2041 ccaaattgga cttgcgacaa cttatgtgcg tgagtacttc caccctggct tcgtagccaa
     2101 acgtatggag cttggagctg tggttggtgc tgcacctaag gaaaatgtgg ttcgtgaaaa
     2161 accagaagca ggtgatgtgg taatcttgct tggtggtaaa acagggcgtg acggtgtcgg
     2221 cggtgcgaca ggttcatcta aggttcaaac ggttgaatct gtggaaacag caggagcaga
     2281 ggtacaaaaa gggaatgcca tcgaagagcg taagattcaa cgtcttttcc gtgctggcaa
     2341 tgtcactcgt ctgatcaaga aatcaaatga cttcggtgca ggtggtgtct gtgttgccat
     2401 cggtgaattg gctgacggtc ttgaaatcga tttggacaag gtgcctctta aataccaagg
     2461 tcttaatggt actgaaattg caatctcaga atctcaagag cgtatgtcag tcgttgttcg
     2521 tccaagtgat gtggatgcct tcatcgcagc ctgcaacaag gaaaatatcg atgcagtcgt
     2581 agttgcgact gtgactgaaa aaccaaatct tgtcatgact tggaatgatg aaacaattgt
     2641 tgatttggaa cgccgtttcc ttgataccaa tggtgtccgt gtcgttgttg atgctaaagt
     2701 cgttgacaag gacttgacag ttccagaagc acgcataaca tcagcagaga cacttgaagc
     2761 agatacgctt aaggtcttgt ctgacctcaa ccatgctagt caaaaaggtc tccaaaccat
     2821 ctttgactca tctgttggtc gttcaaccgt taaccaccca atcggtggtc gttaccaaat
     2881 cacaccgaca gaaagttctg ttcaaaaatt gccagttcaa catggtgtga caacaactgc
     2941 atctgttatg gctcaaggtt acaatgctta tattgcagag tggtcacctt atcacggtgc
     3001 tgcctatgct gttattgaag cgacagctcg cttggtagca acaggtgctg actggtctcg
     3061 tgcacgtttc tcttaccaag agtatttcga gcgtatggat aaacaggcag agcgttttgg
     3121 tcagccagta tcagctcttc ttggttctat tgaggctcag attcaacttg gcttgccatc
     3181 aatcggtggt aaggactcta tgtctggtac tttcgaagac ttgacagtac caccaacctt
     3241 ggtagctttc ggtgtgacaa cagcggacag ccgcaagatt ctctctcctg agtttaaagc
     3301 ggctggtgag aatatctact acattccagg tcaagctatt tcagaagata ttgattttga
     3361 ccttatcaag gctaacttca gtcagtttga agctattcaa actcaacata agattacagc
     3421 tgcctcagcc gttaaatacg gtggtgtcct agaaagtttt gctctcatga cttttggtaa
     3481 ccgtatcggt gcttctgttg aaattgcaga gcttgacagc agcttgacag ctcaactcgg
     3541 aggttttgtc tttacatcag ctgaggaaat tgctgacgcg gtgaaaatcg gtcaaactca
     3601 ggcagacttt acagtcactg tcaatggaaa tgaccttgct ggcgctagcc ttctagcagc
     3661 cttcgaaggc aaattggaag aggtttaccc aacagaattt gaacagacag atgttcttga
     3721 agaagttcct gctgtggtat cagatactgt tatcaaggct aaggaaacaa ttgaaaaacc
     3781 agtggtttac attccagtct tccctggtac caactcagag tacgattcag ctaaggcctt
     3841 tgaacaggtt ggagctagtg tcaacttggt accatttgta accttgaatg aggttgctat
     3901 tgctgagtca gttgacacta tggttgctaa tattgctaag gcaaatatca tcttctttgc
     3961 tggaggtttc tcagcagcgg atgaaccaga tgggtctgct aagtttatcg tcaatatctt
     4021 gcttaacgag aaggtccgcg cagctattga cagcttcatc gaaaaaggtg gccttatcat
     4081 cggtatctgt aatggtttcc aagcccttgt taaatcaggt cttcttccat acggaaactt
     4141 cgaggaagct ggtgagacaa gtccaactct cttctataac gatgctaatc agcatgttgc
     4201 caagatggtt gagactcgta tcgcaaatac caactcacct tggttggcag gagttgaggt
     4261 cggcgatatt catgccattc cagtttcaca tggtgaaggt aaatttgttg tcagcgcttc
     4321 tgaatttgca gagctaagag acaatggtca aatctggagc caatatgtgg actttgacgg
     4381 acaaccatct atggattcta aatacaatcc aaacggctct gtcaatgcca tcgaagggat
     4441 taccagcaag aatggtcaaa tcatcggtaa gatgggacac tcagaacgct gggaagacgg
     4501 actcttccaa aatattcctg gtaacaaaga ccaaaccctc tttgcaagtg ctgtaaaata
     4561 ctttacaggg aagtaaaaag gttcagtgaa cctttttagc cctcgccttg aattagtgaa
     4621 gggcgaggtt agaaagaatc tcgcttaatt tttgagaaat agtaaacacg ggcgataagt
     4681 tgtgcgaaaa actttctatt ttcgctttac ttgacagcct ttgtatcgag gtaaaaatga
     4741 catacgaagt aaaatctcta aatgaagaat gtggagtctt tggtatctgg ggacatcctc
     4801 aggcagctca agtcacttac tttgggcttc atagccttca acatcgcggt caagaaggag
     4861 ctggtattgt ttcaaatgac aatgggaaac tctatggtta tcgaaatgtt gggctccttt
     4921 ctgaagtttt taagaatcaa tctgaattag ataatttaac tgggaatgcg gctattggac
     4981 atgttcggta tgctactgca ggttctgcag atattcgcaa tattcagcct tttctttata
     5041 aatttcatga cgggcaattt gctttatgcc ataatggtaa tttgacaaat gctatttcct
     5101 taaggaaaga attagaaaag caaggtgcaa ttttcaatgc ctcctcagat actgaaattt
     5161 tgatgcactt gattcgtcga agccataact caagtttcat gggaaaggta aaagaagctc
     5221 taaatacagt aaagggtggc tttgcctatc tactgatgac agaaaataaa ctcattgctg
     5281 ctcttgaccc taatgccttt cgtcctttgt caattggaca aatgcaaaat ggtgcctggg
     5341 ttatttccag tgagacctgt gcttttgagg tggtaggcgc aaaatgggtt agagatgttg
     5401 aacctggcga agttattctt atagacgatc gtggtattca atgtgatcgt tatactgatg
     5461 aaacgcaact ggcaatttgt tcgatggaat atgtctattt tgcaaggccg gattcaacta
     5521 tacatggtgt taatgttcat acggttcgaa aaaatatggg aaagcgtctt gcacaagaat
     5581 ttaaacagga tgctgatatt gtaattggtg tcccaaattc gtccttatcg gctgctatgg
     5641 gctttgctga agaatccgga ttaccaaatg agatgggtct tgtaaaaaat cagtatacgc
     5701 agcgaacctt tattcaaccg acacaagaat taagggaaca aggtgttcga atgaaactat
     5761 cagcggtatc tggtgttgtc aaaggaaagc gcgttgttat gattgatgac tcaattgtaa
     5821 gaggaacgac ttctagaagg attgtcggat tattaagaga agcaggagct agtgaagtac
     5881 atgttgctat agctagtcca gaattaaagt atccttgttt ttatggtatt gatattcaaa
     5941 caagacgaga attaatttca gcaaatcacg ctgctgacga agtgtgtgat attattggcg
     6001 cagatagtct gacctatctc tcactagatg ggttgattga atcaattgga ctcgaaacaa
     6061 aagcaccaaa tggtggttta tgcgtagctt actttgatgg acattatcca acaccgcttt
     6121 atgattatga agaagaatac ctcagaagtt tagaagaaaa aacaagtttt tatattcaaa
     6181 aagtgaaata agctctgcta attatataag gagaaaacat gtctgaaaaa aatgcttatg
     6241 caaaatctgg tgttgatgtt gaagctggtt atgaagtggt tgaaagaata aaaaaacacg
     6301 ttgctcgtac agaacgtgcg ggagtcatgg gagctctagg tggctttggt gggatgtttg
     6361 acttgagtaa aacaggagtt agagagcctg ttttggtctc agggacagac ggtgttggca
     6421 caaaactcat gcttgctatc aagtacgaca agcacgacac aatcggtcaa gactgtgttg
     6481 ccatgtgtgt caatgatatt attgcagcag gtgctgagcc cctttacttc cttgactacg
     6541 tagcgacagg aaaaaataat ccagtcaaat ttgaagaagt tgtttctggc gttgcagaag
     6601 gttgtgttca agcaggagca gcactcattg gaggtgagac cgctgaaatg cctgggatgt
     6661 atggcgaaga tgattatgac cttgctggct ttgctgttgg tgtggctgaa aaatctcagc
     6721 tcatcgacgg ttcaaaagta aaggaaggcg atattctttt gggacttgct tcaagtggta
     6781 tccattcaaa cggttattct ttggtacgtc gtgtctttgc tgactatact ggtaaagagc
     6841 tgcttccaga acttgaagga aaacaactta aggatgtcct tcttgagcca actcgtattt
     6901 atgttagagc agctctgcca ttaatcaagg aagaactagt caacggtatc ggccacatca
     6961 caggtggtgg ctttatcgag aatattcctc gtatgtttgc agatgatttg gctgcggaaa
     7021 tcgatgagga caaggtgcca gtgcttccga ttttcaaggc gcttgaaaaa tatggtgaca
     7081 tcaagcacga agaaatgttt gaaatcttca atatgggtgt cggtcttatg ctagctgtta
     7141 gtcctgaaaa tgttaaccgc gtcaaagagc tcttggatga accagtttat gaaatcggtc
     7201 gtatcatcaa gaaagcagac gatagtgtgg tgattaaata agtcactaaa ttttataaga
     7261 aaggagtgtt tagttagttt gcctaactga acacgggatt aaatttctag gaaaaaagat
     7321 aaaatgactt agacgcaagc gtcgttgttc gttttcctat ttttctacga aatttttcgg
     7381 caagccgaat catccctttg tatcgaatat gaaaatcgct gtttttgctt ctggtaatgg
     7441 ttccaacttt caggtcatag cagagcagtt tccagttagt tttgtctttt cagatcatcg
     7501 tgatgcctat gttttagagc gtgctcagaa tttagccatt ccaagcttcg cttttgaact
     7561 caaggagttt gagaataagg cggcttacga gcaagccatc gttgatttgt tggacaaaca
     7621 cgagattgat ttggtctgtc taacgggcta tatgaagatt gttggagaaa ccttactctt
     7681 ggcctatgag gggcgtatta tcaatattca cccagcctac ctgcctgaat ttccaggtgc
     7741 ccacggtatc gaggatgctt gggaagctgg tgttgaccag tctggcgtga ccatccactg
     7801 ggttgactct ggtgtggata ccggtcaggt catccaacaa gtgcgcgtgc cacgcctagc
     7861 agacgatagc ctagaaagct ttgaaacccg tatccatgaa accgaatatc aactctaccc
     7921 agctgtctta gatagcttgg gagtggagag gaaagtaatc tagcctttat cttaaaattt
     7981 taggtaaagt taggaaagat agtaaagaag aattaacgtt ccaaaaagaa aggaattgaa
     8041 cccgtctgga aggcacagaa aaaagataaa catctcctag gcgcaagcgt cttcgtcgag
     8101 tttcctattt ttccttggcc ttctagtcgg acttggtatc ttaaatatga ctaaacgtgc
     8161 tttaatctca gtgtctgaca agtcaggaat tgttgacttt gcaaaagaat tgaaaaactt
     8221 gggttgggat attatctcaa ctggtgggac taaggttgcc cttgataatg ctggcgttga
     8281 gaccattgcc atcgacgatg tgactggatt cccagagatg atggacggtc gtgttaagac
     8341 cctccaccca aacattcacg gtgggctttt ggctcgtcgc gacgctgaca gccaccttca
     8401 ggctgctaag gacaacaata tcgagctgat tgacctcgtg gttgtcaacc tctatccctt
     8461 caaggagact atccttcgtc cagatataac ctatgatttg gcggtggaaa acatcgacat
     8521 cggcggtcca tcaatgcttc gctcagccgc taaaaatcac gctagcgtga ccgttgtggt
     8581 tgacccagcg gattatgcga ctgttttggg agaattggct gacgctggtc agacgacatt
     8641 tgaaacgcgt caacgcttgg cagctaaggt ctttcgtcac acagcagcct acgacgcttt
     8701 gattgctgag tacttcacaa ctcaagtggg agaggctaag cctgaaaaat taaccatcac
     8761 ttacgacctt aaacaggcta tgcgttacgg agaaaatcca caacaagacg ctgatttcta
     8821 tcaaaaagcc ttgccaacag actactcaat tgcttctgct aaacagctca acggaaaaga
     8881 attgtccttc aataatatcc gtgatgccga tgcagcaatc cgtattatcc gtgatttcaa
     8941 agatcgtcca actgttgttg tcctcaaaca catgaaccca tgtggtatcg gacaggctga
     9001 tgatattgag acagcttggg attacgctta tgaagctgac ccagtttcaa tctttggtgg
     9061 aattgtcgtc cttaaccgtg aagttgacgc agcgacagct aagaagatgc accctatctt
     9121 cttggaaatc atcatcgcac catcatactc agaagaagcg ctagctattc tcacaaataa
     9181 aaagaaaaac ttgcgaatcc ttgagttgcc gtttgatgcc caagctgcca gcgaagtgga
     9241 agctgagtac actggcgtag ttggtggact tttggtgcaa aaccaagacg ttgtggctga
     9301 aaatccatct gactggcaag tggtgacaga ccgccaacca acagaacaag aggcgactgc
     9361 ccttgagttt gcctggaagg ctatcaagta tgttaagtct aacgggatta ttattactaa
     9421 cgatcacatg acgcttggac tcggtgcagg tcaaaccaac cgtgtcggct cagtcaagat
     9481 tgctatcgag caggctaaag accaccttga cggtgccgtt ctagcatcag atgccttctt
     9541 cccatttgcg gacaacattg aagaaatcgc tgccgcaggg atcaaagcaa tcatccaacc
     9601 aggtggttca gttcgtgacc aagactctat tgacgccgca aacaaacatg gcttgaccat
     9661 gatcttcaca ggtgtgagac attttaggca ttaataaaaa aatgtggtct aattaaaacc
     9721 acatttttta ttaaaagaaa atataagtta actataattt gttagcttta tcagcattta
     9781 cccatgttcc acgagatccg atttgaacat aaataccatt acttggttga tcaattttta
     9841 atactttata actaccagtg acggtaaaat attcacccac acggagaatt tgatctccta
     9901 aaacttttcc ttggttatct gtttcatcta atggtgtggg atcaatccag tttagtggag
     9961 ttgggtctcc tccggctaat tctttattaa cgatcaaatt attaacaagc tgatctacac
    10021 gaaaaacgcc agggaaggta acagtatcac cgaccttaat ggaaggtttt gtattgtcat
    10081 tttgaaccac agactgtgcg ggctctttaa tgggaatata gcgtcggttt ccagaaaaac
    10141 tgaggtagct aagccatgta taacctccag cagtcacgac tttatcgtaa taaacagatt
    10201 gacctgcttc atagtaagca agatcagggc tatcgatact ggtttgtcct ttgactggta
    10261 atctggttgt aaagtgataa gttccagagg ggtcaaatga agcttgagaa atgtgtttta
    10321 gttgtcttgg gtaggaatga cttgtctgag atgataagtc tttaaaatgg atataaccac
    10381 ttacctgaga ttttggaatt tgacgcttat ggtatctttc agggccttgt ccagcgttgt
    10441 aattatactc ttcgatagtg acagtgtctc cacggatatc agctacccat gctacatgac
    10501 cgtaagcagc atttgactga taagcgtttt tatcaaacca agcgatagcc cctatgcttg
    10561 gtgtcttatt cacaggataa ccctgatttt tcgcgatatg tccccacgtg caggcattac
    10621 cgtagccttt aggtaactga aaaccattag cagagcttaa acgaaaagct gcaaaagaag
    10681 tgcattggcg gatatacatg ttccacgaat cgattccatt gccttttttc cacttactcg
    10741 gatagttatc gcctaaaaca gctgcatgaa caagattttc ctgttgcgaa ataagtgtag
    10801 atggcatagt aggtactaga ccattaaaac ctaaaaggat tactcctgag accaaaaaac
    10861 gatgaaattt tttcattaat tacctcctgt aacttattat tcgaaagcaa gaaacaaaaa
    10921 atgttatttt tagatgatta ttttgaaaat tggctttact tgatattaaa taactgtttg
    10981 attggctaat agagtgttcc ttatttcaag aattgtggtc tactatcaca tttttacaat
    11041 gtttcaatta ttgataacta atacattatg taaaaaagcg aacgtttatg atataatatc
    11101 taattataat acgaaagagg tggatgtttt gaaattactt gttgttggtt caggtggtcg
    11161 tgaacatgcg attgctaaga agttgttagc gtctaagggt gtggatcagg tttttgtggc
    11221 acctggtaat gatggtatga ccttggatgg tctggacttg gtgaatatag tagtttccga
    11281 acattccagg ttgattgcct ttgctaagga gaatgagatt tcttgggcct ttattggtcc
    11341 tgatgatgcg ctagcagctg gtatcgttga tgatttcaac agtgctggac tcagagcttt
    11401 cggtccaaca aaggcagcag cggagctaga gtggtcaaaa gactttgcca aggaaatcat
    11461 ggtcaaatac aatgtcccaa cagcagccta tggcacattt tcagactttg aaaaagccaa
    11521 agcctacatc gaagagcagg gcgcacctat cgtggtcaag gctgatggct tggcggtagg
    11581 taagggtgtg gtcgtggctg aaaccgttga gcaggcggta gaggcggcgc aagagatgct
    11641 tctggacaat aagtttggcg attcaggagc gcgtgtggtt atcgaggaat tcttggatgg
    11701 cgaggagttt tccctctttg cctttgttaa tggtgataag ttctacatca tgccgacagc
    11761 tcaggatcac aagcgtgcct ttgacggaga caaggggcca aacactggtg gtatgggtgc
    11821 ctatgcgcca gttcctcatc tacctcagag cgtggtagac acagcggttg agacgatcgt
    11881 taaacctgtc cttgagggta tgattgccga agggcgtcct tatctaggcg tcctctatgc
    11941 gggacttatc ctgacggctg atggtcctaa ggttatcgag ttcaactcac gtttcggtga
    12001 ccctgaaact cagattatcc tccctcgcct gacttccgat ttcgctcaga atattgacga
    12061 tatcatgatg ggcattgagc cttacatcac ttggcagaag gacggtgtga ctttgggtgt
    12121 tgtcgttgcc tcagaaggct atccactcga ttatgagaaa ggtgtgccac tgcctgaaaa
    12181 gaccgacggt gatatcatca cctactacgc aggagttaag tttgcagaaa atagcgagct
    12241 acttctctca aacggaggac gtgtctatat gctcgtcacc acagaagaca gcgtcaaagc
    12301 agggcaggac aaaatctata cccaactggc ccaacaagac ccaacaggtc tcttctaccg
    12361 aaatgacatc ggaagcaaag ctataagaga ataagactaa ccattcaaat attagcgtca
    12421 gcaatcaacg accgaaggtc gctccaaaaa gaaagaagaa aggaactgaa cacgggacta
    12481 atttcctagg aaaaaaataa aactacctgg acgcaagcat catcgttagt tttctgcgtc
    12541 gtaggcgtct gatgctaaag cacctagtcg ccctgacgta ggttcgtctt cgaaatcaaa
    12601 gatttcggac ttaccgcata tttttctacg gaaagtttcg acaagtcgaa acgccccttt
    12661 gtatcttaat tatgaaaact ccaattattt ctattatcat gggctccaaa tctgactggg
    12721 caaccatgca aaaaacggct gaagttctag acaactttgg cattgcttac gagaagaaag
    12781 tcgtctctgc tcaccgcacg cccgacctca tgttcaagca tgctgaggaa gcacgtggtc
    12841 gtggcatcaa aatcatcatc gcaggagcag gtggggcagc tcacttgcct ggtatggtgg
    12901 cagctaaaac aacgctgcct gttatcggtg tgcctgtcaa atcacgtgcc ctttcaggtt
    12961 tggattcact ctattcgatt gtgcagatgc ctggcggtgt gcctgtggca accatggcta
    13021 tcggagaagc gggagcgact aacgctgccc tgacagccct ccgtattctc tccatcgagg
    13081 atcaaaatct ggctgatgcg ctagctcatt tccatgagga acaaggaaaa atcgcagagg
    13141 agtcaagtgg tgaactcatt taagaccatt gggattatcg gtggtggtca gctggggcag
    13201 atgatggcga ttgcggctat ctacatgggc cataaggtca tcacgctgga tcctgctaac
    13261 gactgtcctg ccgcccgcgt tagcgaggtg attgtggcac cttacgatga tgttgaggct
    13321 ttgggacaat tagctgcgcg ttgcgatgtt ttgacctatg agtttgagaa tgtcgatgcc
    13381 gatggtctgg atgccgttgt gtcagctggt cagctaccgc aggggactga tctgctccgc
    13441 atttcccaaa accgtatcgt tgaaaaagac tttctgacaa ataaggctgg tgtgactgtc
    13501 gctccttata aggtggtgac gtctagcctt gacctagagg ggcttgattt gaccaagacc
    13561 tatgtcctca agacagcgac aggtggttat gacggtcatg ggcaaaaggt tattcgctcc
    13621 gcagaagacc taccagaggc gcaacaatta gccaactcag ctcagtgtgt cttggaagag
    13681 tttgtcaatt ttgaccttga aatctcagtt atcgtgtctg gaaatggtca ggatgtgacg
    13741 gtctttcccg ttcaggaaaa tatccaccgc aataatatcc tgtcaaaaac catcgtgcca
    13801 gctcgtatct cagatcaact ggctgataag gctaagaaaa tggcggtgca gattgccaag
    13861 aaactccagc tgtcaggaac tctctgtgtg gaaatgtttg cgaccgcaga tgatatcatt
    13921 gtcaatgaaa ttgccccacg tccccacaac tcagggcact actcaatcga ggcttgcgac
    13981 ttttcacagt ttgacaccca cattttgggc gtgctaggag cgccgctccc aaccatccac
    14041 ctccatgctc cagccgtcat gctcaatgtc ctcggacaac acgtccagca ggcgattgac
    14101 tatgtgactg aaaaccctag cgcccacctc cacatgtatg gtaaactaga agcgaaacat
    14161 aaccgcaaaa tgggacacgt gacggtgttt gcagaagatg cggatgaggt ggaggagttt
    14221 tagaacagga gtagattggt aagatggagt taagggaatt taaagcgtgg ttattaaaaa
    14281 accattttga agaagatgag ttatacaagg aatctataaa gtgttatcaa gtggaagcat
    14341 ataaggctgc ctatattttt tcgtatttag caaatcataa gttcattgct aaattagcta
    14401 ttgactacag tggtgtccca tcaaatttta tgtctaaatg tcccgatgat gaagttagaa
    14461 agtcgagttg gcaaaaacgt gtgaattcct taaaaaatga agatgagtgg gagcagacgg
    14521 ttaacgaaaa attaattcaa gcatgggaag gtgataatgt attcaaatta cctttcaaaa
    14581 ttaaagaaaa atattctcat atgagggtat tgcgtaataa tgcagcccat gcgaaagact
    14641 gtcgaatttc tgaatcaaca gttttagaat tatggaatga aattgagtat atatatcctt
    14701 actttgtaat caacggaacg attgatgcat ggatggaaga tttagaaaaa ctagttagat
    14761 attcaaatag tagcatagaa tctcaaaacc aattacagaa taaatttgga gatttttcta
    14821 ctgaaaataa gaccttaatt gttaaaaaga ttatacaaaa atatttggta gattctgaat
    14881 ggg
//
LOCUS       DAUGHY010000038        13597 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.038, whole genome shotgun sequence.
ACCESSION   DAUGHY010000038 DAUGHY010000000
VERSION     DAUGHY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13597
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.038"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(373..1818)
                     /locus_tag="VH752_001264"
     CDS             complement(373..1818)
                     /locus_tag="VH752_001264"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEQ8289613.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAV
                     NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
     gene            complement(2000..2710)
                     /locus_tag="VH752_001265"
     CDS             complement(2000..2710)
                     /locus_tag="VH752_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEQ8289614.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLGHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIIALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            complement(2841..3338)
                     /locus_tag="VH752_001266"
     CDS             complement(2841..3338)
                     /locus_tag="VH752_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEQ8289615.1"
                     /translation="MSYFEHFMAANQAYVALHGTAHLPLKPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFTNEGF
                     AKHIHEHLGMDVSGQDFLPFQDVEDSVREDMTKIRASSLISDDVVINGAVYDVDTGKM
                     TQVME"
     gene            complement(3527..4888)
                     /gene="radA"
                     /locus_tag="VH752_001267"
     CDS             complement(3527..4888)
                     /gene="radA"
                     /locus_tag="VH752_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEQ8289616.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYASGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKA
                     VFS"
     gene            complement(4969..5415)
                     /locus_tag="VH752_001268"
     CDS             complement(4969..5415)
                     /locus_tag="VH752_001268"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEQ8289617.1"
                     /translation="MTKIRGFELVSSFTNPDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIILINSVGVIDADYYGNEANEGHIFAQ
                     MQNITDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            complement(5525..6292)
                     /locus_tag="VH752_001269"
     CDS             complement(5525..6292)
                     /locus_tag="VH752_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEQ8289618.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWEKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            complement(6410..8194)
                     /locus_tag="VH752_001270"
     CDS             complement(6410..8194)
                     /locus_tag="VH752_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289619.1"
                     /translation="MKTARFFWFYFKRYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLVQVVTNIALY
                     IGLVWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQEDTMTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAIVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQTISQDMVETAARATHIHDFIMSLPKGYNTYVSDDDNVFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            complement(8197..9903)
                     /locus_tag="VH752_001271"
     CDS             complement(8197..9903)
                     /locus_tag="VH752_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289620.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPILFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIEPGQMVGIVGATGSGKSTLVQLIPRLFDPQDGQILLGGKPI
                     KTLSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     DSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            complement(9896..10345)
                     /locus_tag="VH752_001272"
     CDS             complement(9896..10345)
                     /locus_tag="VH752_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ8289621.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
     gene            10643..11659
                     /locus_tag="VH752_001273"
     CDS             10643..11659
                     /locus_tag="VH752_001273"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ8289622.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     RNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
     gene            11692..12591
                     /gene="galU"
                     /locus_tag="VH752_001274"
     CDS             11692..12591
                     /gene="galU"
                     /locus_tag="VH752_001274"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEQ8289623.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIR
                     QSHPRGLGDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATI
                     AVMRVPHEDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIF
                     SILEKQEPGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKH
                     PQVKDDLKAYIIQLGKALEKTKP"
     gene            complement(12690..13361)
                     /locus_tag="VH752_001275"
     CDS             complement(12690..13361)
                     /locus_tag="VH752_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEQ8289624.1"
                     /translation="MTQLLKRYPITIFLLGLTGLIFIAMQVVYGHLATGAQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSRRFLLLYVL
                     SGVMGNAFTFWLTPETVAAGASTSLFGLFAAIVVLSFLGKNQALKDLGKSYQTLIVVN
                     LLMNLFMPNVSMAGHIGGVVGGALLSIVFPTKMRVITVKKTKRMLALVSYGIILVGVL
                     VLGFL"
BASE COUNT         4150 a         2964 c         2455 g         4028 t
ORIGIN      
        1 ttattttagc atattttatg acaatgatta caatattatt tgattacaat tgattagcta
       61 agtgagtact aaactagtct ctttcagcat ataccaaaaa aatattgtac actaattgat
      121 tcataaaaac catttcttta aatcacacta atcaaaacct caccgtttgc tgtcattatc
      181 acgtaagcat ctcaccacct ctctttcaaa tccaatctcc aaaatagaaa taaactatac
      241 tattttttac tttaccaagg tagcaagctc agataacgat aaaaaagact gaagaactta
      301 atcttcaatc tttcgttgtc gtgacttttt aactccttaa agcgtttaaa aataaaggtg
      361 aacgatacag ccttatagca tgtttttgat gtgctcaatg gatttatcac gtcccaataa
      421 ataaatcgta tttggtaatt ctgggccatg catttcacca gatactgcaa tacgaatagg
      481 cataaagagg ttttttcctt tgattcctgt ttctttttga acagccttaa tttgtgggaa
      541 aatattttct ggtttgaagt cttcatctga catagcttcc aacttagcct tgaaagcttg
      601 caagactgtc gaaactgttt cacctgccat cacttctttt tcggcttctg ttaattcagg
      661 gaaatcagaa aagaaaaggt cagtcaatgg aataatctca tctgcagatt ttaattgagg
      721 tttgtataat tctactagct tctcagcttt ttctgtcaaa cggcctgcct cttcaaggaa
      781 aggtttgcag agggcataaa ctgtttcaaa atccgcatgt ttaagatact cattgctcat
      841 ccaatccatt ttcttctggt caaaggcagc tggcgacttg ctaaggcgat tttcatcaaa
      901 aagggcaatc agttgctcac gagagaagat ttcctcttct ccgccaggat tccagccaag
      961 aagggcgatg aagttaaaga cagcttcagg catataacct tttttgcggt aatcttcaat
     1021 gaattggagg gtattggtgt cgcgttttga taatttttta cccgtttcag aattgatgat
     1081 aagagtcata tgaccaaact ctggcgcttc ccagccaaga gcttcataga ccatcaattg
     1141 ttttggcgta ttggcaatat ggtcatcgcc acggataacg tgagaaattt gcatatcatg
     1201 atcatcaaca acaacggcaa agttataagt tgggtaccca tctttctttt gaataaccca
     1261 gtcaccaccg atgttaccac cttcaaactc gatatcgcct ttaaccatgt cagtccattt
     1321 gtaaataccg gactcattaa cagcaagacg gactgttgga acgatacctg ctgcttcacg
     1381 ctcagcaatg tacttagctt tttcgtcagc tgacatacca ataaattcat tgatatagcg
     1441 aggcgtttcg ccagctgctt cttggcgttc acgctctgct gccaattctt cctcagtcac
     1501 gtaagactta taggcttttc cttcagctaa caattgatca atatattgtt gataaagtgc
     1561 tagacgttca gactggcggt aattttcatg cgtttctggg ctttcatccc aatccattcc
     1621 taaccatttt aggttttcaa gttgagaacg ttcaccatct tcaacatgac gtttacggtc
     1681 agtatcttca atacgaatga taaaagtacc tccgtgacga cgggcataaa ggtaattaaa
     1741 tagggctgtg cgggcattcc cgatatgtaa aagccctgtt ggacttggtg cataacgcac
     1801 acgaattggt ttagacatgc ttcttctcct gttacatata tcactcagaa acctgtggca
     1861 cgaaaaacaa gcaacaaatg atgatttgag cttgccaaga ccttttcaga cttcttctaa
     1921 aatctttctt attatagcat attttacttg cttaggcgaa aaaaatcagc cctaaaaagc
     1981 tgattttatg ttattttgac taatttgaac tagttggtag ataacgttta agagtcccag
     2041 caaattgctc aaggttaaga ctttggatat aaatctcacc atgacctcta aaggtattaa
     2101 caactccttc acctgtacca attgactgca taaagccatt ttctaaatga atatcataat
     2161 caagttctct gctccatgct acaacatggg cattatcaat agtcattgtg ccaccatcaa
     2221 gtgtgatttt cttgatagat ccaaacgagt tagcaagtaa ggtacccaag ccttcagttg
     2281 tcatgacaaa aagtccgcct tgaccaccaa aaagtgcttt tccaatattt tgacgttcca
     2341 ttttgtactg tgctgaacca tctagggcta gaaaggcacc atcatttaga cgatattgtt
     2401 tttcacctag ttctaaagca ataatctgtc ctggtgtatt gggtgctaat gctaatttac
     2461 catctccatt tgacatagct tgtgtaatga acattgattc accagaaacc atggaacggc
     2521 cgatggctcc aactaatttt cctaaaccag atcccttacc attcaatttg gtattcaagg
     2581 ttacattctc agtatgataa accatacttc cttgttgtaa gtaaactgat cccccatgtc
     2641 ccagatcaat ctcaactagt ggaaattgca tgttttgatc aatcgtaaat ctcattctat
     2701 tgtcgaccat cttactctcc ttgtttgtat gaataacttg atacaattat tataacgctt
     2761 ccctatttct ttgtctaata ttttcaataa tttttaaaaa taaaaaagag ctgaattcag
     2821 ctctacaact tggctactaa ctattccatt acttgtgtca tctttccagt atccacatcg
     2881 tagacggcac cgttaataac gacatcatca gagataagag aggaggctct tatcttagtc
     2941 atatcctcgc ggacactgtc ttccacatct tggaagggaa ggaaatcttg ccctgaaaca
     3001 tccataccta agtgctcatg aatatgcttg gcaaagcctt cattagtaaa agtctgcgcg
     3061 ccacaatctg tatggtggag aaccacaatc tctcgcgttc ccatttgctg ctgagaaata
     3121 accagcgacc gaatcatgtc ttcggtgaca cgtccgccag cattccttaa aatatgagca
     3181 tctcctagag ccagccccag agcttgggcc acatgtagcc gtgaatccat acaagtcaca
     3241 atcgctactt tagtcttggg ttttaagggc aaatgagctg tcccatgcaa ggccacataa
     3301 gcctgattgg cagccataaa atgttcaaaa taagacatca ataaactcct tttgagaaac
     3361 agcagagcta tttttgctgt tagtttgtct tatcttaaca tcttttatgg aaaatagctg
     3421 gattaacctc ttagacggtc ggtatcgttc gaaaagttaa aaaaacaaat gattgggata
     3481 ctttcttgac aaatttcctc ctcatttgct cttagtttat gatcctctag ctaaaaactg
     3541 cctttaaaac ctgaccaacc gtagtcactc cgacaacctc aataccttga gggatatcaa
     3601 tgccttgcaa ggcattttta ggagcataga ccttggtaaa tccaagcttt gctgcttcat
     3661 taatccgttg ctcaatacgg gtcactcgcc gaatctcacc tgttaacccg atctcgccta
     3721 agaaagcttc ttgagggctg gttggttttt ccttataact ggatgcgata gccacagcga
     3781 ctgccaaatc aatggcaggt tcgtctagtt tgacaccacc agccgacttg aggtaggcat
     3841 cttgattttg taagagtaaa ccacagcgct tttctaaaac tgccattatt aggcttacgc
     3901 gattgaaatc aagtcccgta gtggttcgtc tggcattgcc aaaaacggtt ggtgtcacta
     3961 aagattgcac ctctgctaag ataggacgac ttccttccat cgttacaacg acagccgatc
     4021 ctgttgcccc atctaagcgt tcctctaaaa agacttgact aggatttaag acctctacca
     4081 agccgccaga ctgcatttca aagatgccaa tctcgttagt ggagccaaag cggtttttca
     4141 cagcccgaag aatgcgaaag gtgtgatgcc tttccccttc aaagtagaga acggtatcca
     4201 ccatgtgttc taacatacga ggcccagcca aggttccttc cttggtgaca tgacccacaa
     4261 taaaggttgc aatgttgttc gtctttgcaa gctgcatcaa ttctgcagtg acttctctca
     4321 cctgactgac tgatccttga acacctgtaa tatcagggct catgatggtc tgaatggagt
     4381 caataattaa aaaatctggt ttaatatttt caatttctgt gcgaatagct tgcatattgg
     4441 tttcggcata gaggtaaaat tcattgtcaa tatcacccag acgctcactc cgcagtttaa
     4501 tctgctctgc tgattcttcc ccagacgcat agagaaccgt tcccttatta gcgagctgcg
     4561 tagatacctg caagagtaag gtggattttc caatacctgg atcaccacca attaaaatca
     4621 agcttccagg aaccacaccc cctccaagaa ctctattaaa ttcagacatg tcagtctgtg
     4681 ttctgtgata actaatatta tccacatctt ttagtttaac cggtctggac ttttctccag
     4741 ctagactaac acgggcattt ttgacttctt tgacctctac ttcttccaca aaagaagacc
     4801 aggccgagca atttggacag cgccctaaat actttggcga ctggtagcca cactcctgac
     4861 agataaaggt tgcttttttc tttgccatag taatctccct cccctagaca agacaaaccc
     4921 actcttaaag aataggtctg ctgtcataag catttttgtt aaatcgcttt actgccctgt
     4981 cgaaccaaaa ccacctgttc gttcacctct ggcttggtca ccatcagcaa ttaaaaaagg
     5041 cataaaaacc ccctgaacga tcctctcacc cacagctaat gtcacagggt gatctgtaat
     5101 gttttgcatt tgtgcaaaaa tatgtccttc attagcctca tttccataat aatctgcatc
     5161 aatcacacct actgaattaa tcaggataat cccttttttt cgtggatttg acgaacgatc
     5221 atagaggtaa agcacctcac catcttgcat ataggcctta acccctgttg gtaccaactt
     5281 aatctcccct ggggcaatgg ttacagcctc agcaacagac aggtcataac cagctgcatg
     5341 agtcgtctca cgttttggta gtaaatcagg attagtaaag cttgaaacaa gctcgaaacc
     5401 acgaattttt gtcattatga taggtatatc ctttcaagtc aatcatatcc tcttattata
     5461 gactattcgt aaaagaatag caaaaaagcg gtcaacttta aacggaccac ttttaatcat
     5521 gccatcacat ctcatcccca cgataactaa agcggatgtg ggtgaaatca ttatccaaat
     5581 ccttgtctgc cataaaagct ttggcatctt ttttcacttg aaccaagtca ataccttgca
     5641 ttttggcagc atagacacag ccacaatagc attgacggta aatatcatac tcctcacaca
     5701 tttcaacgga tctgcgataa ccgttgtttt tcttgaaatc acttggcagg tatttggtgg
     5761 tgtaaacctt ttgcacatcg atgcccacat cattaatggt ttgcgaattt ttatgaggac
     5821 tgatggttag ggcactggca aagtagtcaa aacctagttc cactgctttt tgagcggtct
     5881 tgtctagtcg gtaatcaaaa cagacacggc aacggtcccc accttctggt tcctcttcta
     5941 agcctcgcac ttgtcgaacg tactcattgg ggacatagtc cgcttcgaga aactgaacag
     6001 tattgcctgt tttagcgttg aactcggaga caaactgttg tgtcacatag gctctgcgat
     6061 ggtattcgtc cttagggtga atattagaat tggcaaagta aaccgtaatg tcagcaaact
     6121 gcgtcaggta ttctagggtg taagtggagc atggggcgca gcagacatgc attaaaatac
     6181 tgggacgcac ggattccttc tcccagacct tggccatctg ttgcatgacc ctgtcataat
     6241 ttatcttttg attgggattc atgttagcga gaatctcttg taaatcaatc attgtggcta
     6301 ctttctaatt gttttttctc ttgcttgcta ttataccata tcaggaaaga aagcttgact
     6361 gctttattgt tcaccaaaaa actccgctaa gacgcggagc tgataccatc tattcaaaga
     6421 caaattgatt gtgatacaat tcggcataaa agcctttttg atgcaatagc tcatgatgat
     6481 ttccttgctc aatgaccttg ccatctttca acacaataat gtgatctgca tttaaaatgg
     6541 ttttgaggcg gtgagcaatg acaaagctag ttcgacctgc cacgatagct tccatggccc
     6601 gttgaatttt actttcggta accgtatcaa catttgaagt ggcctcatcc aaaatcaaca
     6661 cttcagggtc agtcagtagc gtcctagcaa tagaaatcaa ctgcttttga cctgttgaaa
     6721 agacattgtc atcatctgag acataggtat tgtacccttt tggtaaggac atgataaagt
     6781 catgaatatg ggtcgcacgc gcagcagttt caaccatgtc ttgactaatg gtctgatcac
     6841 caaaacgaat attatccgta atggttcctg aaaaaagaac tgactcttgc aacacaatcc
     6901 ctaccttttg acgaagacta tccaaatcgt agtcacgaat atcacggcca tcaaaggtaa
     6961 tcgaacctgc atccacatcg tagaaacggt taatcaagtt cataatagtg gtctttccag
     7021 aacctgtcgg tccaaccacg gcaatcattt tgcccttggg tgcaacgatt gacacatctg
     7081 ataaaacttt ttgcccagga agatacccaa aatcgacgtg gttaatcgcc actgcttctt
     7141 ttaagctggt gaacgctggt gcattttgtg gacgaacttc ttcggtttca tcaaacattt
     7201 cttgaatacg gtgagcaccg gtaaaggcca gctgcaattc tccccaacta gacgcgattt
     7261 gcatcatggg ttggtaatat tgctgggaat attgtacaaa agtaaccact aaaccaagcg
     7321 ctgccgctgc tggcatagat ttgtcactga ggacaattgt tgaaccgaca aaaatcacga
     7381 tagccgtgtt aataaggctc attccattca tgactggaaa taattgtcct gagaacagac
     7441 ggcgtttgaa ggtggcttgt cgaacccttt cattatgctt taaaaaggct gtcatcgtat
     7501 cttcttggac accttgtaca ataatagcct tttgtcctga aatggtttca tccataaaag
     7561 catttaaagc actgacttct tgctgttgga tattggtgta ttttcttgcc aaacggatgt
     7621 taatcactaa aaaaatgaga gcaactgggg ttgatgcgat ggttaacaaa gctaaacggc
     7681 tatcttgcct aaacatcatc cagaccaggc cgatgtaaag ggcaatatta gtcaccactt
     7741 gaaccaagga ttggttcagc gagttttgaa tattatccaa atcactcgtg aaacgagaaa
     7801 gaatctcccc atctttatgg cggtcaaaaa aggcgacggt taaacgttct aatttaccaa
     7861 acaagccctt gcgcatgcgg ttggtcgaat gtgagacaac acgtgtaagt aagaaactat
     7921 atattagatt agctaaaaca gtaaagaaat aagtcatcaa aagcttaaac atcacggcat
     7981 taaaagctga taaatcaggg ctaaaatgtg tctgaccact catcttagca acgtaataag
     8041 cctgaccgat ttttcccaac tcagtcaagg actcccctaa gaagacagga gcttttactt
     8101 gtaaataagt tgctaagata acggcaacag caatgacagt aaacgagaaa cgatagcgtt
     8161 taaaataaaa ccagaaaaaa cgtgctgttt tcatcactac tcctcccttc ctttttgtgt
     8221 ctcataaatt tcacgataaa tcgcattatt agccaccaac tctgcgtgtg ttccttctcc
     8281 aattaactgt ccttggtcaa ggactaagat cttatctgcc ttaactaccg atgaaatttt
     8341 ttgagcaaca ataactgtag ttgtaccttc taatttatga cttaaggctt cttgaacgcg
     8401 tttttcagat tttgcatcaa gagctgaagt ggaatcgtct aatataagga ttttgggatg
     8461 attaatgaca ccacgtgcaa tagacagacg ttgcttttgt ccgccagaca agttactacc
     8521 tcgctcttca acttggctct cataacggct atccatgcga tcaataaatt cttttgcttg
     8581 cgcgatttga gcggcttttt gcatagcatc aatatcagct tttgctgagc cttggcgaag
     8641 gttatcagct atcgttcctg aaaagagaat agccttttgc aacacgatgg aaaccgattg
     8701 gcgcagggtt gtttgactta gggttttaat cggctttcct cctaacaaaa tttggccatc
     8761 ttgaggatca aataagcgag ggatgagctg tacaagcgtt gatttcccag atcctgttgc
     8821 accaacgatg ccgaccattt gcccaggttc aatagcaaaa gaaatgtgct tcaaggttgg
     8881 ctcatcatca ttaggatatg taaaactaac atcgtcaaat acaatgcttc ctgagatggt
     8941 ttcttccttt tcattttcaa atgtcatggc aggttctgta gacaatattt gcctaattct
     9001 agccatagaa acaaaagcac gactaacttg catccccatg gaaccaacca caataattga
     9061 aaacataatt tgcatcatat aagtcatgaa agaggcaata ttcccaatca ctgttggatc
     9121 tgtttcgacc atggtcgaaa caacatttat agaaacataa actgccaaat aagacaccaa
     9181 cattaaagcc ggctgcatta gtgaaaaacc ataaccaata gataaattta acgccaaaag
     9241 atcattggac gtttctttaa atttagtgta ttgttgttgt tcttgtacaa atgatttaac
     9301 cacgcgaacg ccacggagat tttccttagc gatgcgatta atcttatcca ttaaccgttg
     9361 aaatttacca aaacgaggac ccatttgacg catcaccaaa cccatgatga gggcaattaa
     9421 gataaccatt acaacaatga cccaccataa ttgagggaat gtctgaactg ccatgataaa
     9481 cgcgccaata aaaaggatcg gcaaacgaaa gagaatttga aacatcatca tgactaaact
     9541 ttggatttga ttgatatcat tagttagcct aaccacaaga ttaccagcat taaaagcttc
     9601 aatattggca tatgaaaagt cttggatttt tcgaaaggtc ttctccctca tatcagcaga
     9661 tacaccttga gctattttag cagctaaaac cgtattaatc gctccagaca ttaaccctaa
     9721 aagggcaata ataagtaatg gtttcccaac ctcataaatt ttctcttgat tattttgaat
     9781 aacagcgctt agcacagatt taagataatg tggttgcaag agactacttg caacaacccc
     9841 aataaccata aaaaatgagc ctaaagcata ccatttataa cgtaagattg cgtctttaag
     9901 catcgttgtc tcctttataa gttagcatat tttgcttgag ctgttcaatt acttttttcg
     9961 ctgtcagcaa ctcttctttt gtaatttctt ttaaaaaata gtgctcgata tccttacgac
    10021 actcccgcaa caaaggcaat ttatctctac caacctgcgc taacactact tgcttataac
    10081 gcttatccac ttgggaaggc atcacattga taaacccatt tttgaccata cgtttcacta
    10141 aatgactagc aactgacttt gagatacgaa gttgtttttc aatatctttg acaaatattt
    10201 cttgattttg gtgtttagct aaaaaaacaa gaacataacc ttgaggacct gctagatgct
    10261 ctacatcata tttttttgca atctcatcac taatttgttc gatttgatgt atcaattcac
    10321 gtaaatcacc tatcacttgt gacatctctt ccctccttct tttccttaat agtttctatg
    10381 ataagtattt tagttctcta gataactatt gtcaattaaa atatctttat ttttaaaaat
    10441 aagtttccga acttaaataa ctaaaaagaa caaggcaaag ccatgacaac agctcacctg
    10501 atgaagctta ttaaacaaca ttattaatgt agctagctct cttgagaacg ccttattagc
    10561 caagaccata taccttttct aatagagcta atcaaggtgt tatgttataa tagagtgact
    10621 agctcttgaa ggagaattta gaatgacaaa acaaaaagta gctattttag gacctggttc
    10681 ttggggaact gctctttctc aggttcttaa tgacaatggc catgacgtcc gtttatgggg
    10741 aaatattcct gatcaaattg aagagataaa taccaagcat accaatagac actacttcaa
    10801 agacattgtg ttagacaaaa acattacggc aactcttgat ttaggtcaag ccctatcaga
    10861 tgttgatgcg gtgctttttg ttgtgccaac taaagtaaca cgattagttg ctagacaagt
    10921 tgccgccatt ttagatcata aagtagtggt catgcatgcc tcaaaaggtc tagaaccaga
    10981 gacccacgaa cgtttatcaa ctatcctaga agaagaaatt ccagctcatt ttcgtagtga
    11041 agtggtcgtt gtatcaggtc ctagtcatgc tgaagaaact atcgttcgcg atattactct
    11101 catcacggca gcctcaaaag atattgaagc tgcaaagtat gttcagtccc tctttagtaa
    11161 ccactacttc cgactttata ccaataccga cgtcatcgga gttgaaacag ctggtgccct
    11221 caaaaacatt attgctgttg gagcaggagc tctccatgga cttgggtatg gagataatgc
    11281 caaagcagcg gtaattactc gtgggttagc tgaaatcacc cgtctaggcg tcaagcttgg
    11341 tgctgacccg ttaacctaca gtgggttatc tggtgttggt gatctaattg taacaggtac
    11401 ttctgtccat tcacgtaact ggcgggctgg agctgccctt ggacgtggtg aaaaattaga
    11461 agatattgaa cgcaacatgg gtatggttat cgaaggcatc gcaacaacta aggtagccta
    11521 tgaaattgcc caagacctcg gagtttacat gccaattaca acagctattt ataagtccat
    11581 ctacgagggc gctgatatca aagaaagcat cctcggcatg atgtcaaatg aatttcgttc
    11641 tgaaaatgaa tggcattaag ccatacgtca ctaaaataag aggaggtgct gatgaccaaa
    11701 gtcagaaaag ctattattcc cgcagcaggt ttaggcacac gttttttacc tgcaaccaag
    11761 gcccttgcca aggagatgct acctattgtt gataaaccaa ctattcaatt tatcgttgaa
    11821 gaagctctaa aatcaggtat cgaagaaatt cttatcgtta ctggaaaatc caaacgctcc
    11881 atagaagatc attttgattc taattttgaa ttagaatata atttacaggc taaaggaaaa
    11941 atcgaacttc taaagttagt agacgagacc acttctatta accttcactt tattcgacag
    12001 agtcatccaa gaggattagg agacgcagtt cttcaagcaa agacctttgt tggcaatgaa
    12061 ccttttgttg tcatgctagg tgatgatctg atggatatta caaatccaaa tgtaaaacca
    12121 ttaaccaaac aattgatcga tgactatgaa gaaactcatg ctgcaaccat tgccgtcatg
    12181 agggttcctc acgaggatgt ctctaactac ggtatcattg ctcctcaagc aaaagcagta
    12241 aaaggactct atagtgtgga tacttttgtt gaaaaaccac aacctcaaga tgcacctagt
    12301 gatttagcaa ttattggacg ttacttgctc acaccagaaa tttttagtat tttagaaaaa
    12361 caagaacctg gtgcaggcaa tgaagttcaa ctgactgatg ctattgatac cctcaataag
    12421 acacagcgtg tctttgctcg tgagtttaaa gggaaacgct atgatgttgg cgataaattt
    12481 gggtttatga aaacttcttt ggattatgcc ctgaaacacc ctcaagtcaa agatgacttg
    12541 aaagcctaca tcatccaact gggcaaagca ctagagaaga caaaaccata aaaataagga
    12601 gggaaaatcc ctccttattt ttatggtttt taagtcattt catcctacta gccatctgag
    12661 aagtaacagt gctaaccact cacttatttt tataaaaacc caagcactaa cacaccaacc
    12721 aaaataatac catagctcac taaagccaac attcgcttgg ttttcttcac tgttatcact
    12781 ctcatcttag ttggaaaaac tatactcagt aacgcaccac ccacgacacc gccaatatgg
    12841 cctgccatac tcacgtttgg cataaaaaga ttcatcagta aattgactac aattaacgtc
    12901 tggtatgact tgcccaaatc tttcaacgct tggttcttac ccaaaaaact caatactaca
    12961 atagcagcaa aaagtccaaa taacgacgtc gatgctcctg cagctactgt ttcaggagtc
    13021 aaccaaaaag taaaggcatt gcccataaca cctgataaaa cataaagtaa taaaaaacgg
    13081 cgtgagcccc agaggtcttc gacgatttga ccaacaaaat atagtgttaa tccattgaca
    13141 aaaaaatgac caaagccgat atgaataaat atcggagtca ccaatcgcca caactggtct
    13201 ggcattgctt ttactagtaa tccaaacata cctccaactt gataaatagc ttgagcacca
    13261 gttgctaaat gtccatatac cacttgcatt gctataaaaa ttaagcctgt taaccctaaa
    13321 aggaaaatag taataggata tcgttttaat aactgtgtca tctaagataa acctcctaaa
    13381 cagcaatatc ataaatcgtt tgatttggca atcgtatttg cccgctgata agcttgattg
    13441 ctaacaacat ccactaataa agcttgatca ttttgacgtt ttaagtctac atccatagtt
    13501 cgtaaagact agaagagttc tttgagaatc tcttttttca tcatcttctt atcataccat
    13561 aaattaggaa aaaggttcaa ggctgacaag aaactag
//
LOCUS       DAUGHY010000039        13117 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.039, whole genome shotgun sequence.
ACCESSION   DAUGHY010000039 DAUGHY010000000
VERSION     DAUGHY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.039"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..409
                     /locus_tag="VH752_001276"
     CDS             <1..409
                     /locus_tag="VH752_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ8289625.1"
                     /translation="VLMGGQSESVEFTKDTQTGMSGQTTPQVETEDTKEPGVLMGGQS
                     ESVEFTKDTQTGMSGFSETVTIVEDTRPKLVFHFDNNEPKVEENREKPTKNITPILPA
                     TGDIENVLAFLGILILSVLSIFSLLKNKQNNKV"
     gene            633..1481
                     /locus_tag="VH752_001277"
                     /pseudo
     CDS             633..1481
                     /locus_tag="VH752_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407229.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            1767..4037
                     /locus_tag="VH752_001278"
     CDS             1767..4037
                     /locus_tag="VH752_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus ancillary protein 1"
                     /protein_id="HEQ8289626.1"
                     /translation="MNNKKLQKKQDAPRVSNRKPKQLTVTLVGVFLMFLTLVSSMRGA
                     QSIFGEEKRIEEVSVPKIKSPDDAYPWYGYDSYDSSHPYYERFKVAHDLRVNLNGSKS
                     YQVYCFNINSHYPNRKNAFSKQWFKRVDGTGDVFTNYAQTPKIRGESLNNKLLSIMYN
                     AYPKNANGYMDKIEPLNAILVTQQAVWYYSDSSYGNIKTLWASELKDGKIDFEQVKLM
                     REAYSKLISDDLEETSKNKLPQGSKLNIFVPQDKSVQNLLSAEYVPESPPAPGQSPEP
                     PVQTKKTSVIIRKYAEGDYSKLLEGATLRLTGEDILDFQEKVFQSNGTGEKIELSNGT
                     YTLTETSSPDGYKIAEPIKFRVVNKKVFIVQKDGSQVENPNKEVAEPYSVEAYSDMQD
                     SNYINPETFTPYGKFYYAKNKDKSSQVVYCFNADLHSPPESEDGGGTIDPDISTMKEV
                     KYTHTAGSDLFKYALRPRDTNPEDFLKHIKKVIEKGYNKKGDSYNGLTETQFRAATQL
                     AIYYFTDSTDLKTLKTYNNGKGYHGFESMDEKTLAVTKELINYAQDNSAPQLTNLDFF
                     VPNNSKYQSLIGTEYHPDDLVDVIRMEDKKQEVIPVTHSLTVKKTVVGELGDKTKGFQ
                     FELELKDKTGQPIVNTLKTNNQDLVAKDGKYSFNLKHGDTIRIEGLPTGYSYTLKETE
                     AKDYIVTVDNKVSQEAQSASENVTADKEVTFENRKDLVPPTGFITDGGTYLWLLLLVP
                     FGLLVWFFGRKGLKND"
     gene            4030..4551
                     /gene="lepB"
                     /locus_tag="VH752_001279"
     CDS             4030..4551
                     /gene="lepB"
                     /locus_tag="VH752_001279"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ8289627.1"
                     /translation="MTNYLNRLNENPLFKAFIRLVLKISIIGFLGYILFQYVFGVMIV
                     NTNQMSPAVSAGDGVLYYRLTDRYHINDVVVYEVDNTLKVGRIAAQAGDEVSFTQEGG
                     LLINGHPPEKEVPYLTYPHSSGPNFPYKVPTGTYFILNDYREERLDSRYYGALPINQI
                     KGKISTLLRVRGI"
     gene            4573..5601
                     /locus_tag="VH752_001280"
     CDS             4573..5601
                     /locus_tag="VH752_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QVPTGV class sortase B protein-sorting
                     domain-containing protein"
                     /protein_id="HEQ8289628.1"
                     /translation="MKKNKLLLATAILATALGTASLNQNVKAETAGVVSSGQLTIKKS
                     ITNFNDDTLLMPKTDYTFSVNPDSAATGTESNLPIKPGIAVNNQDIKVSYSNTDKTSG
                     KEKQVVVDFMKVTFPSVGIYRYVVTENKGTAEGVTYDDTKWLVDVYVGNNEKGGLEPK
                     YIVSKKGDSATKEPIQFNNSFETTSLKIEKEVTGNTGDHKKAFNFTLTLQPNEYYEAS
                     SVVKIEENGQTKDVKIGEAYKFTLNDSQSVILSKLPVGINYKVEEAEANQGGYTTTAT
                     LKDGEKLSTYNLGQEHKTDKTADEIVVTNNRDTQVPTGVVGTLAPFAVLSIVAIGGVI
                     YITKRKKA"
     gene            5617..6342
                     /gene="srtB"
                     /locus_tag="VH752_001281"
     CDS             5617..6342
                     /gene="srtB"
                     /locus_tag="VH752_001281"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HEQ8289629.1"
                     /translation="MMMTIVQVINKAIDTLILIFCLVVLFLAGFGLWDSYHLYQQADA
                     SNFKKFKTAQQQPKFEDLLALNEDVIGWLNIPGTHIDYPLVQGKTNLEYINKAVDGSV
                     AMSGSLFLDTRNHNDFTDDYSLIYGHHMAGNAMFGEIPKFLKKDFFNKHNKAIIETKE
                     RKKLTVTIFACLKTDAFDQLVFNPNAITNQDQQRQLVDYISKRSKQFKPVKLKHHTKF
                     VAFSTCENFSTDNRVIVVGTIQE"
     gene            6359..6946
                     /locus_tag="VH752_001282"
     CDS             6359..6946
                     /locus_tag="VH752_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEQ8289630.1"
                     /translation="MRKYWKMLFSVVMILTMLAFNQTVLAKDSTVQTSISVENVLERA
                     GDSTPFSIALESIDAMKTIEEITIAGSGKASFSPLTFTTVGQYTYRVYQKPSQNKDYQ
                     ADTTVFDVLVYVTYDEDGTLVAKVISRRAGDEEKSAITFKPKRLVKPIPPRQPNIPKT
                     PLPLAGEVKSLLGILSIVLLGLLVLLYVKKLKSKL"
     gene            complement(7105..8310)
                     /locus_tag="VH752_001283"
     CDS             complement(7105..8310)
                     /locus_tag="VH752_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEQ8289631.1"
                     /translation="MVIFDLKHVQTLHSLSQLPISVMSQDKALIQVYGNDDYLLCYYQ
                     FLKHLAIPQAAQDVIFYEGLFEESFMIFPLCHYIIAIGPFYPYSLNKDYQEQLANNFL
                     KHSSHRSKEELLSYMALVPHFPINNVRNLLIAIDAFFDTQFETTCQQTIHQLLQHSKQ
                     MTADPDIIHRLKHISKASSQLPPVLEHLNHIMDLVKLGNPQLLKQEINRIPLSSITSS
                     SISALRAEKNLTVIYLTRLLEFSFVENTDVAKHYSLVKYYMALNEEASDLLKVLRIRC
                     AAIIHFSESLTNKSISDKRQMYNSVLHYVDSHLYSKLKVSDIAKRLYVSESHLRSVFK
                     KYSNVSLQHYILSTKIKEAQLLLKRGIPVGEVAKSLYFYDTTHFHKIFKKYTGISSKD
                     YLAKYRDNI"
     gene            8695..12180
                     /locus_tag="VH752_001284"
     CDS             8695..12180
                     /locus_tag="VH752_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ8289632.1"
                     /translation="MTQKNSYKLSFLLSLTGFILGLLLVFIGLSGVSVGHAETRNGAN
                     KQGSFEIKKVDQNNKPLPGATFSLTSKDGKGTSVQTFTSNDKGIVDAQNLQPGTYTLK
                     EETAPDGYDKTSRTWTVTVYENGYTKLVENPYNGEIISKAGSKDVSSSLQLENPKMSV
                     VSKYGKTEVSSGAADFYRNHAAYFKMSFELKQKDKSETINPGDTFVLQLDRRLNPKGI
                     SQDIPKIIYDSANSPLAIGKYHAENHQLIYTFTDYIAGLDKVQLSAELSLFLENKEVL
                     ENTSISNFKSTIGGQEITYKGTVNVLYGNESTKESNYITNGLSNVGGSIESYNTETGE
                     FVWYVYVNPNRTNIPYATMNLWGFGRARSNTSDLENDANTSSAELGEIQVYEVPEGEK
                     LPSSYGVDVTKLTLRTDITAGLGNGFQMTKRQRIDFGNNIQNKAFIIKVTGKTDQSGK
                     PLVVQSNLASFRGASEYAAFTPVGGNVYFQNEIALSPSKGSGSGKSEFTKPSITVANL
                     KRVAQLRFKKMSTDNVPLPEAAFELRSSNGNSQKLEASSNTQGEVHFKDLTSGTYDLY
                     ETKAPKGYQQVTEKLATVTVDTTKPAEEMVTWGSPHSSVKVEANKEVTIVNHKETLTF
                     SGKKIWENDRPDQRPAKIQVQLLQNGQKMPNQIQEVTKDNDWSYHFKDLPKYDAKNQE
                     YKYSVEEVNVPDGYKVSYLGNDIFNTRETEFVFEQNNFNLEFGNAEIKGQSGSKIIDE
                     DTLTSFKGKKIWKNDTAENRPQAIQVQLYADGVAVEGQTKFISGSGNEWSFEFKNLKK
                     YNGTGNDIIYSVKEVTVPTGYDVTYSANDIINTKREVITQQGPKLEIEETLPLESGAS
                     GGTTTVEDSRPVDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDIDGKELAGATMELR
                     DSSGKTISTWISDGQVKDFYLMPGKYTFVETAAPDGYEIATAITFTVNEQGQVTVNGK
                     ATKGDTHIVMVDAYKPTKGSGQVIDIEEKLPDEQGHSGSTTEIEDSKSSDLIIGGQGE
                     VVDTTEDTQSGMTGHSGSTTEIEDSKSSDVIIGGQGQVVETTEDTQTGMYGDSGCKTE
                     VEDTKLVQSFHFDNKEPESNSEIPKKDKPKSNTSLPATGEKQHNMFFWMVTSCSLISS
                     VFVISLKSKKRLSSC"
     gene            complement(12447..13112)
                     /locus_tag="VH752_001285"
     CDS             complement(12447..13112)
                     /locus_tag="VH752_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289633.1"
                     /translation="MKRCNKYLFTSLLAASMLFSSYKSVHAHDNIDEKGKVHLYWQGN
                     YYVDNYVDYTKALTADEKSIEWTVTFNSAKEQWVYPDFSVFLPKGVKAPTEITYEHHY
                     WDGTVHSEKHNNTQWHYDWKSQQTNFNQEFDKFPGYTGWSPSLDKFYKLKNDGKFSHV
                     LVDTYGRQSHTYFSHKMVWKFKTELEEDYKNKWDKLPFIAGIKQNNPLAASFPSYKGE
                     FGE"
BASE COUNT         4515 a         2131 c         2619 g         3852 t
ORIGIN      
        1 agtgttgatg ggaggccaaa gtgagtctgt tgaatttact aaagacactc aaacaggcat
       61 gagtggtcaa acaactcctc aggttgagac agaagacacg aaagagccag gagtgttgat
      121 gggaggtcaa agtgagtctg ttgaatttac taaagacact caaacaggca tgagcggttt
      181 cagtgaaaca gtgaccattg ttgaagatac gcgtccgaag ttagtgttcc attttgacaa
      241 taatgagccc aaagtggaag agaatcggga aaagcctaca aaaaatataa cacctatcct
      301 tcctgcaaca ggagatattg agaatgtttt ggcctttctt ggaatcctta ttttgtcagt
      361 actttctatt tttagccttt taaaaaacaa acaaaacaat aaagtctgac gtaaaagtgt
      421 tccataagaa ataatgcgca aatatgctga atagtggata actgctacag tcaagacaag
      481 tgttaattca aaaggtacta cttatacatc gtataataga cttacaagaa atcaagttat
      541 tgttaaacgt cgtaaagctt aacgctttca aaagtcgaaa tcaatcgaga ctgtctttag
      601 gcagtctcga tttttattga taaggaactg caatgacaga aagactaaaa aatctaggga
      661 tactcttatt atttttattg ggaacagcca tttttcttta ccctacgcta agtagtcagt
      721 ggaatgccta tcgtgatcgt caattgttat caacttatca caaacaagtg actcaaaaga
      781 agccctcaga aatggaggag gtgtggcaaa aggctaaggc gtataacgct aggcttggga
      841 ttcagcccgt tccagatgct ttttcattta gggatggcat tcatgataaa aactacgaaa
      901 gcctacttca aattgaaaac aatgatatta tgggataagt tgaagtgccc tctattaaag
      961 tgacgttacc catataccac tatacgacag acgaggtgct aactaaggga gcaggtcact
     1021 tagttggaag tgccttacca gttggtggcg acgggactca cacggttatt tctgctcata
     1081 gaggccttcc atcggcagaa atgtttacta atttgaattt agtgaaaagg ggagatacct
     1141 tttactttcg tgtgttaaat aaagtattgg cttataaagt tgatcagata ctaactgttg
     1201 aaccagatca agtcacatct ctttctgggg taatgggaaa agatcatgct actctggtta
     1261 cctgcacacc atatggtgtt aataccaagc gtctattggt cagaggtcat agaattacat
     1321 atcattacaa aaagtatcaa caggctaaaa aagcaatgaa actcgtggat aaatcaagaa
     1381 tgtggacaga agtagtgtgt gctgctttcg gggtggttat agctattatt ttagtattta
     1441 tgtattcaaa gatgactgat aaaaaagtga ttaaaaacta acttgccctg aaccttcttt
     1501 atcgtattga aactgtcata aaagtaaaat aatctggtag ttactatccc ctttgatgaa
     1561 gtttaattaa atctcatcaa gagaaaaata ttaggcgatt acaaatgatt ttcccaaaaa
     1621 ttattttttg gaacgcataa tatggaaaaa aagatgataa gaacaactgt tttaaacatc
     1681 aaataaatga tatagtagct atgaaatagc attgtgactg tccggtaaag ggatgggact
     1741 gaagatttta ttcagaagga gagggaatga ataacaaaaa attgcaaaag aagcaagatg
     1801 ctcctcgggt atcaaacaga aagccaaaac aattaactgt cactttagtg ggagtatttt
     1861 taatgttttt gaccttggta agttccatga gaggtgctca aagcatattt ggagaggaaa
     1921 agagaattga agaagtcagt gttcctaaaa taaaaagtcc agatgatgcc tacccttggt
     1981 atggctatga ttcatatgac tctagtcatc cttactatga acgttttaaa gtagcacatg
     2041 atttaagggt taatttaaat ggaagtaaga gctaccaagt atattgcttt aatatcaatt
     2101 ctcattatcc gaatagaaaa aatgcttttt ctaaacaatg gtttaagaga gttgatggga
     2161 caggtgatgt gttcacaaat tatgctcaga cacctaagat tcgtggagaa tcattgaata
     2221 ataaactttt aagtattatg tacaacgctt atcctaaaaa tgctaatggc tatatggata
     2281 agatagaacc attaaatgct attttagtaa ctcaacaagc tgtttggtac tattctgaca
     2341 gttcttatgg taatataaaa acgttatggg catctgagct taaagacgga aaaatagatt
     2401 ttgaacaagt aaaattaatg cgtgaagctt actcaaaact aattagtgat gatttagaag
     2461 aaacatctaa aaataagcta cctcaaggat ctaaactgaa tatttttgtt ccgcaagata
     2521 aatctgttca aaatttatta agtgcagagt acgtgcctga atcccctccg gcaccaggtc
     2581 agtctccaga accgccagtg caaacaaaaa aaacatcagt cattatcaga aaatatgcgg
     2641 aaggtgacta ctctaaactt ctagagggag caactttgcg tttaacaggg gaagatatcc
     2701 tagattttca agaaaaagtc ttccaaagta atggaacagg agaaaagatt gaattatcaa
     2761 atgggactta taccttaaca gaaacatcat ctccagatgg atataaaatt gcggagccga
     2821 ttaagtttag agtagtgaat aaaaaagtat ttatcgtcca aaaagatggt tctcaagtgg
     2881 aaaatccaaa caaagaagta gcagagccat actcagtgga agcgtacagc gatatgcaag
     2941 atagtaacta tattaatcca gaaacgttca ctccttatgg gaaattttat tacgctaaaa
     3001 ataaggataa aagttcacaa gttgtctact gttttaatgc tgatttacac tctccacctg
     3061 aatcagagga tgggggagga actatagatc ctgatattag tacgatgaaa gaagtcaagt
     3121 acacacatac ggcaggtagt gatttgttta aatacgcgct aagaccgaga gatacaaatc
     3181 cagaagactt cttaaagcac attaaaaaag taattgaaaa aggctacaat aaaaaaggtg
     3241 atagctataa tggattaaca gaaacacagt ttcgcgcggc tactcagctt gctatctatt
     3301 actttacaga cagcactgac ttaaaaacct taaaaactta taacaatggg aaaggttacc
     3361 atggatttga atctatggat gaaaaaaccc tagctgtaac aaaagaatta attaattacg
     3421 ctcaagataa tagtgcccct caactaacaa atcttgattt cttcgtacct aataatagca
     3481 aataccaatc tcttattggg acagaatacc atccagatga tttggttgac gtgattcgta
     3541 tggaagataa aaagcaagaa gttattccag taactcacag tttgacagtg aaaaaaacag
     3601 tagtcggtga gttgggagat aaaactaaag gcttccaatt tgaacttgag ttgaaagata
     3661 aaactggaca gcctattgtt aacactctaa aaactaataa tcaagattta gtagctaaag
     3721 atgggaaata ttcatttaat ctaaagcatg gtgacaccat aagaatagaa ggattaccga
     3781 cgggatattc ttatactctg aaagagactg aagctaagga ttatatagta accgttgata
     3841 acaaagttag tcaagaagct caatcagcaa gtgagaatgt cacagcagac aaagaagtca
     3901 cttttgaaaa ccgtaaagat cttgtcccac caactggttt tattactgat ggtggaacct
     3961 atctgtggtt attattgctt gtcccatttg gtttgttagt gtggttcttt ggtcgtaaag
     4021 gactaaaaaa tgactaatta cctaaatcgc ttaaatgaga atccactatt taaagctttc
     4081 atacggttag tacttaagat ttctattatt ggatttctag gttacatttt atttcagtat
     4141 gtttttggcg tcatgattgt taacacaaat cagatgagtc ctgctgtaag tgctggtgat
     4201 ggagtcttat attatcgttt gactgatcgc tatcatatta atgatgtggt ggtctatgag
     4261 gttgataaca ctttgaaagt tggtcgaatt gccgctcaag ctggcgatga ggttagtttt
     4321 acgcaagaag gaggactgtt gattaatggg catccaccag aaaaagaggt cccttacctg
     4381 acgtatcctc actcaagtgg tccaaacttt ccctataaag ttcctacggg tacgtatttc
     4441 atattgaatg attatcgtga agaacgtttg gacagtcgtt attatggggc gttacccatc
     4501 aatcaaatca aagggaaaat ctcaactcta ttaagagtga gaggaattta aaaaaatttg
     4561 gagagagaga aaatgaaaaa aaataaatta ttacttgcta ctgcaatctt agcaactgct
     4621 ttaggaacag cttctttaaa tcaaaacgta aaagctgaga cggcaggggt tgttagcagt
     4681 ggtcaattaa caataaaaaa atcaattaca aattttaatg atgatacact tttgatgcct
     4741 aagacagact atacttttag cgttaatccg gatagtgcgg ctacaggtac tgaaagtaat
     4801 ttaccaatta aaccaggtat tgctgttaac aatcaagata ttaaggtttc ttattctaat
     4861 actgataaga catcaggtaa agaaaaacaa gttgttgttg actttatgaa agttactttt
     4921 cctagcgttg gtatttaccg ttatgttgtt accgagaata aagggacagc agaaggagtt
     4981 acatatgatg atacaaaatg gttagttgac gtctatgttg gtaataatga aaagggaggt
     5041 cttgaaccaa agtatattgt atctaaaaaa ggagattctg ctactaaaga accaatccag
     5101 tttaataatt cattcgaaac aacgtcatta aaaattgaaa aggaagttac tggtaataca
     5161 ggagatcata aaaaagcatt taactttaca ttaacattgc aaccaaatga atactatgag
     5221 gcaagttcgg ttgtgaaaat tgaagagaac ggacaaacga aagatgtgaa aattggggag
     5281 gcatataagt ttactttgaa cgatagtcag agtgtgatat tgtctaaatt accagttggt
     5341 attaattata aagttgaaga agcagaagct aatcaaggtg gatatactac aacagcaact
     5401 ttaaaagatg gagaaaagtt atctacttat aacttaggtc aggaacataa aacagacaag
     5461 actgctgatg aaatcgttgt cacaaataac cgtgacactc aagttccaac tggtgttgta
     5521 ggcacccttg ctccatttgc agttcttagc attgtggcta ttggtggagt tatctatatt
     5581 acaaaacgta aaaaagctta agtgattagt caaagaatga tgatgacaat tgtacaggtt
     5641 atcaataaag ccattgatac tctcattctt atcttttgtt tagtcgtact atttttagct
     5701 ggttttggtt tgtgggattc ttatcatctc tatcaacaag cagacgcttc taatttcaaa
     5761 aaatttaaaa cagctcaaca acagcctaaa tttgaagact tgttagcttt gaatgaggat
     5821 gtcattggtt ggttaaatat cccggggact catattgatt atcctctagt tcagggaaaa
     5881 acgaatttag agtatattaa taaagcagtt gatggcagtg ttgccatgtc tggtagttta
     5941 tttttagata cacggaatca taatgatttt acggacgatt actctctgat ttatggccat
     6001 catatggcag gtaatgccat gtttggcgaa attccaaaat ttttaaaaaa ggattttttc
     6061 aacaaacata ataaagctat cattgaaaca aaagagagaa aaaaactaac cgtcactatt
     6121 tttgcttgtc tcaagacaga tgcctttgac cagttagttt ttaatcctaa tgctattacc
     6181 aatcaagacc aacaaaggca gctcgttgat tatatcagta aaagatcaaa acaatttaaa
     6241 cctgttaaat tgaagcatca tacaaagttc gttgcttttt caacgtgtga aaatttttct
     6301 actgacaatc gtgttatcgt tgtcggtact attcaagaat aacgaaagga ggagactttt
     6361 gagaaaatat tggaaaatgt tattttctgt cgtaatgata ttaaccatgc tggcctttaa
     6421 tcagactgtt ttagcaaaag acagcactgt tcaaactagc attagtgtcg aaaatgtctt
     6481 agagagagca ggcgatagta ccccattttc gattgcatta gaatcaattg atgcgatgaa
     6541 aacaatagaa gaaataacaa ttgctggttc tggaaaagca agcttttccc ctctgacctt
     6601 cacaacagtt gggcaatata cttatcgtgt ttatcagaag ccttcacaaa ataaagatta
     6661 tcaagcagat actactgtat ttgacgttct tgtctatgtg acctatgatg aagatgggac
     6721 tctagtcgca aaagttattt ctcgaagggc tggagacgaa gaaaaatcag cgattacttt
     6781 taagcccaaa cggttagtaa aaccaatacc gcctagacaa cctaacatcc ctaaaacccc
     6841 attaccatta gctggtgaag taaaaagttt attgggtatc ttaagtatcg tattactggg
     6901 gttactagtt cttctttatg ttaaaaaact gaagagtaag ctataaaacc catttttaag
     6961 agtcacacat ttttagttgc tctattttta taatgaaact tattgacatc ttcatagtct
     7021 tgcttgactt tggagatgtt ttctatgctg ataggttttt gtaccatata agataatgat
     7081 tgatgaaaag agtgtcttga attcttaaat attatctcgg tatttagcaa gatagtcttt
     7141 tgaagaaata cccgtgtatt ttttaaagat tttatgaaaa tgggtagtgt cataaaaata
     7201 taagctttta gccacttctc caacaggaat tcctcgtttt aagagtagtt gagcttcttt
     7261 gatttttgta cttagaatat aatgttgtaa ggaaacattt gagtattttt taaagactga
     7321 acgtaagtga gattcggaaa catataggcg cttagcgata tcagatacct ttaatttgga
     7381 atacaggtga ctatcgacat aatgaagcac actattgtac atttgacgtt tatcagaaat
     7441 acttttattg gttaatgatt cggaaaaatg gatgatggct gcacagcgaa ttctcaaaac
     7501 tttgagcaag tcactcgctt cttcatttaa ggccatgtag tatttgacaa ggctataatg
     7561 ctttgctacg tcagtatttt ctacaaaact gaattccagt aacctagtta aatagataac
     7621 agtgaggttc ttttccgccc ttagagcaga aatagaagat gaggtgatac ttgataaggg
     7681 gatgcgattg atttcttgct tgagcaattg tggattgcct agctttacca gatccataat
     7741 atgatttagg tgctctaaaa caggcggtag ttggctagat gctttgctaa tatgcttaag
     7801 gcgatgaatg atatcaggat cagcagtcat ctgttttgaa tgctgcaaca attgatgaat
     7861 tgtttgttgg caagtcgtct caaattgtgt gtcaaaaaaa gcgtcaatag ctatcaaaag
     7921 gttccgcaca ttattaattg gaaaatgtgg gacaagtgcc atataggata agagctcttc
     7981 tttgctacga tgagaagaat gttttaaaaa attattagct aattgttcct gatagtcttt
     8041 attaagtgaa taagggtaga aaggtccaat ggcaataatg tagtgacaaa gaggaaaaat
     8101 cataaaggac tcttcaaata aaccctcata aaaaataaca tcttgtgcag cttgaggaat
     8161 agctagatgc tttaaaaatt gatagtaaca taataaatag tcgtcattac catatacttg
     8221 aataagtgcc ttatcttgtg acatcactga aataggtaat tgagacaagc tgtgtaatgt
     8281 ttgcacatgt tttaaatcga atataaccat ttgatagatt gccccccatc cttagttgta
     8341 tcattttcct tagacttgta aaagctttag taaatgttaa tggtgaaaat tcctatttgg
     8401 taggcacact aaaaggtctt cctaatagta aactctctct ccatcatctc ctgccttaat
     8461 tatactatga atagctaagc agataaagat aaaggatttg tacgttaagc gcttgaaaaa
     8521 gaaagagtta cagataatga cataaaaaac gctaaaaaac catcaaaata aatactctga
     8581 ccataagatg tagacttgac aactgaaaat ggtaaaataa ctatttgaca gttggcctgt
     8641 agtctttagt tttggacata ggctgtcgct tatgaatgtg gagagagaaa ataaatgaca
     8701 caaaaaaata gctataagtt aagcttcctg ttatccctaa caggatttat tttaggttta
     8761 ttattggttt ttataggatt gtccggagta tcagtaggac atgcggaaac aagaaatgga
     8821 gcaaacaaac aaggatcttt tgaaatcaag aaagtcgacc aaaacaataa gcctttaccg
     8881 ggagcaactt tttcactgac atcaaaggat ggcaagggaa catctgttca aacgttcact
     8941 tcaaatgata aaggtattgt agatgctcaa aatctccaac cagggactta taccttaaaa
     9001 gaagaaacag caccagatgg ttatgataaa accagccgga cttggacagt gactgtttat
     9061 gagaacggct ataccaagtt ggttgaaaat ccctataatg gggaaatcat cagtaaagca
     9121 gggtcaaaag atgttagtag ttctttacag ttggaaaatc ccaaaatgtc agttgtttct
     9181 aaatatggga aaacagaggt tagtagtggc gcagcggatt tctaccgcaa ccatgccgcc
     9241 tattttaaaa tgtcttttga gttgaaacaa aaggataaat ctgaaacaat caacccaggt
     9301 gatacctttg tgttacagct ggatagacgt ctcaatccta aaggtatcag tcaagatatc
     9361 cctaaaatca tttacgacag tgcaaatagt ccgcttgcga ttggaaaata ccatgctgag
     9421 aaccatcaac ttatctatac tttcacagat tatattgcgg gtttagataa agtccagttg
     9481 tctgcagaat tgagcttatt cctagagaat aaggaagtgt tggaaaatac tagtatctca
     9541 aattttaaga gtaccatagg tgggcaggag atcacctata aaggaacggt taatgttctt
     9601 tatggaaatg agagcactaa agaaagcaat tatattacta atggattgag caatgtgggt
     9661 gggagtattg aaagctacaa caccgaaacg ggagaatttg tctggtatgt ttatgtcaat
     9721 ccaaaccgta ccaatattcc ttatgcgacc atgaatttat ggggatttgg aagggctcgt
     9781 tcaaatacaa gcgacttaga aaacgacgct aatacaagta gtgctgagct tggagagatt
     9841 caggtctatg aagtacctga aggagaaaaa ttaccatcaa gttatggggt tgatgttaca
     9901 aaacttactt taagaacgga tatcacagca ggcctaggaa atggttttca aatgaccaaa
     9961 cgtcagcgaa ttgactttgg aaataatatc caaaataaag catttatcat caaagtaaca
    10021 gggaaaacag accaatctgg taagccattg gttgttcaat ccaatttggc aagttttcgt
    10081 ggtgcttctg aatatgctgc ttttactcca gttggaggaa atgtctactt ccaaaacgaa
    10141 attgccttgt ctccttctaa gggtagtggt tctgggaaaa gtgaatttac taagccctct
    10201 attacagtag caaatctaaa acgagtggct cagcttcgct ttaagaaaat gtcaactgac
    10261 aatgtgccat tgccagaagc ggcttttgag ctgcgttcat caaatggtaa tagtcagaaa
    10321 ttagaagcca gttcaaacac acaaggagag gttcacttta aggacctgac ctcgggcaca
    10381 tatgacctgt atgaaacaaa agcgccaaaa ggttatcagc aggtgacaga gaaattggcg
    10441 accgttactg ttgatactac caaacctgct gaggaaatgg tcacttgggg aagcccacat
    10501 tcgtctgtaa aagtagaagc taacaaagaa gtcacgattg tcaaccataa agaaaccctt
    10561 acgttttcag ggaagaaaat ttgggagaat gacagaccag atcaacgccc agcaaagatt
    10621 caagtgcaac tgttgcaaaa tggtcaaaag atgcctaacc agattcaaga agtaacgaag
    10681 gataacgatt ggtcttatca cttcaaagac ttgcctaagt acgatgccaa gaatcaggag
    10741 tataagtact cagttgaaga agtaaatgtt ccagacggct acaaggtgtc gtatttagga
    10801 aatgatatat ttaacaccag agaaacagaa tttgtgtttg aacagaataa ctttaacctt
    10861 gaatttggaa atgctgaaat aaaaggtcaa tctgggtcaa aaatcattga tgaagacacg
    10921 ctaacgtctt tcaaaggtaa gaaaatttgg aaaaatgata cggcagaaaa tcgtccccaa
    10981 gccattcaag tgcagcttta tgctgatgga gtggctgtgg aaggtcaaac caaatttatt
    11041 tctggctcag gtaatgagtg gtcatttgag tttaaaaact tgaagaagta taatggaaca
    11101 ggtaatgaca tcatttactc agttaaagaa gtaactgttc caacaggtta tgatgtgact
    11161 tactcagcta atgatattat taataccaaa cgtgaggtta ttacacaaca aggaccgaaa
    11221 ctagagattg aagaaacgct tccgctagaa tcaggtgctt caggcggtac cactactgtc
    11281 gaagactcac gcccagttga taccttatca ggtttatcaa gtgagcaagg tcagtccggt
    11341 gatatgacaa ttgaagaaga tagtgctacc catattaaat tctcaaaacg tgatattgac
    11401 ggcaaagagt tagctggtgc aactatggag ttgcgtgatt catctggtaa aactattagt
    11461 acatggattt cagatggaca agtgaaagat ttctacctga tgccaggaaa atatacattt
    11521 gtcgaaaccg cagcaccaga cggttatgag atagcaactg ctattacctt tacagttaat
    11581 gagcaaggtc aggttactgt aaatggcaaa gcaactaaag gtgacactca tattgtcatg
    11641 gttgatgctt acaagccaac taagggttca ggtcaggtta ttgatattga agaaaagctt
    11701 ccagacgagc aaggtcattc tggttcaact actgaaatag aagacagtaa atcttcagac
    11761 cttatcattg gcggtcaagg tgaagttgtt gacacaacag aagacacaca aagtggtatg
    11821 acgggccatt ctggctcaac tactgaaata gaagatagca agtcttcaga cgttatcatt
    11881 ggtggtcagg ggcaggttgt cgagacaaca gaggataccc aaactggcat gtacggggat
    11941 tctggttgta aaacggaagt cgaagatact aaactagtac aatccttcca ctttgataac
    12001 aaggaaccag aaagtaactc tgagattcct aaaaaagata agccaaagag taatactagt
    12061 ttaccagcaa ctggtgagaa gcaacataat atgttctttt ggatggttac ttcttgctca
    12121 cttattagta gtgtttttgt aatatcacta aaatccaaaa aacgcctatc atcatgttaa
    12181 gataagctgt tggtgacaat agcgaaaaag ggtccactta ttagtgggcc tctttttctg
    12241 gaatcgatcg tctcggttca ctagataagt cattttaagt agttcaaaaa ggccgagcct
    12301 taaaaggttt tctgcctttt taatcttgtt gggtacttac gaaagtattt cggccacttc
    12361 aagcataata cagtcagagt ctgagcaatc atgttgtctc agactctgac tgtattatgc
    12421 aaaagatgat ggactggcag cttcatttac tcaccgaact cccctttata acttgggaat
    12481 gatgctgcta atgggttgtt ttgtttaatg cctgctataa acggtaattt atcccattta
    12541 tttttataat cttcttcaag ctcagtttta aacttccaaa ccattttatg agaaaaataa
    12601 gtgtgtgatt gacggccata ggtgtctact aaaacgtgag aaaattttcc gtcgtttttt
    12661 agtttgtaaa atttatctag agaaggactc cagccagtat aaccagggaa tttatcaaat
    12721 tcttgattaa aatttgtttg ttggcttttc caatcataat gccattgtgt attattgtgt
    12781 ttttcagaat gaactgtacc gtcccaataa tgatgctcat aagttatctc tgtaggagct
    12841 tttacaccct taggaagaaa aactgagaaa tcagggtaaa cccattgttc tttagctgaa
    12901 ttaaaggtta cagtccattc gatacttttt tcatctgcag ttaatgcttt agtatagtcg
    12961 acatagttat caacatagta atttccctgc cagtaaaggt gcactttacc tttctcatca
    13021 atgttatcat gggcatgtac tgatttatac gaactaaaca gcatcgaagc tgctagtagt
    13081 gaagtgaaga gatatttatt acatcgtttc atatcat
//
LOCUS       DAUGHY010000040        12879 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.040, whole genome shotgun sequence.
ACCESSION   DAUGHY010000040 DAUGHY010000000
VERSION     DAUGHY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12879
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.040"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(22..1290)
                     /locus_tag="VH752_001286"
     CDS             complement(22..1290)
                     /locus_tag="VH752_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEQ8289634.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYHCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSIPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYHKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NAGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYNTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKEPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPHFLTQKSLIDLDTNLLMQKALALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVIDYTTSDSYRNFTLDKSDRIIIT
                     GKKRYLSIAINQQIQSKLNSLIKNTCGKSN"
     gene            1380..2246
                     /locus_tag="VH752_001287"
     CDS             1380..2246
                     /locus_tag="VH752_001287"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEQ8289635.1"
                     /translation="MKRIIVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQSPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLPLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEGSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKRDLILGVAYESHLNYLTKSFKK
                     LLEEKNVISKIS"
     gene            2224..2781
                     /locus_tag="VH752_001288"
     CDS             2224..2781
                     /locus_tag="VH752_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ8289636.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRVPVGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLAICLILHLVYERAMQSSKK
                     PKAIITITVIAALSKLTINLIKYLLLAYIGTQLPLSVSFWVALGKIGGSFGTSFVTLV
                     AVPLLYPVIKNLKEKVTIGQAKRGF"
     gene            2915..4459
                     /locus_tag="VH752_001289"
     CDS             2915..4459
                     /locus_tag="VH752_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ8289637.1"
                     /translation="MSILEVKQLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGHLQPDEGKVEWSKYVTAGHLDQHTVLESGQTVRDVLRTAFDELFKTENRIN
                     EIYASMADDKADIAVLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMESDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNHLDAEHIEWLKRYLQHYENAFVLISHDI
                     SFLNDVINIVYHVENQSLVRYTGDYYQFQAVYEMKQSQLEAAYERQQKEIANLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPNFEFKQARTPSRFIFQTKNLVIGY
                     DYPLTKEPLNITFERNQKIAIVGANGIGKSTLLKSLLGVIEPLEGHIVTGDFLEVGYF
                     EQEVTGVNRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLILDEPTNHLDIDAKNELKRALKAYKGSILMVCHEPDFYNGWVTDTW
                     DFSKLT"
     gene            4823..6046
                     /locus_tag="VH752_001290"
     CDS             4823..6046
                     /locus_tag="VH752_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ8289638.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLEMQFPKHTRRLIKDAKQRGVETYRASQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDKAYLHLAKVNIPQKLDQYRQQLILINQDITRTQAHQKKRLKKL
                     EDQKASLERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPNVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYKIRKQLKNKH"
     gene            complement(6088..7650)
                     /gene="guaA"
                     /locus_tag="VH752_001291"
     CDS             complement(6088..7650)
                     /gene="guaA"
                     /locus_tag="VH752_001291"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEQ8289639.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELREINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSGTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILQNFAISICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            7817..9250
                     /locus_tag="VH752_001292"
     CDS             7817..9250
                     /locus_tag="VH752_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289640.1"
                     /translation="MLRKKRILGLFRPVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKEPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKRYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDFFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLTSWFMEPIHFPPNSPVIVAGNIIN"
     gene            9333..10031
                     /locus_tag="VH752_001293"
     CDS             9333..10031
                     /locus_tag="VH752_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8289641.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEHFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            10120..10461
                     /locus_tag="VH752_001294"
     CDS             10120..10461
                     /locus_tag="VH752_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEQ8289642.1"
                     /translation="MNIMEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEI
                     ADEFGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEK
                     ISILTSIDNRE"
     gene            10476..12038
                     /gene="ffh"
                     /locus_tag="VH752_001295"
     CDS             10476..12038
                     /gene="ffh"
                     /locus_tag="VH752_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEQ8289643.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENRNDYVLIDTAGRLQIDEKLMEELRDVKALAQPDEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKVDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMSKMMKDMGINPNNLP
                     KTMPAGMPDMSSLEGMMGQGGMPELSGLGGDMDMSQLFGKGFKGKIGQFAMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(12173..12871)
                     /locus_tag="VH752_001296"
     CDS             complement(12173..12871)
                     /locus_tag="VH752_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8289644.1"
                     /translation="MFSGHQLKTVRLSKGITQSELGRLLHVNKMTISNWEKGKNIPNE
                     KHLNALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNHQIDKKSKDLI
                     DKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFGDSMEPTYLNG
                     EVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSDKFAPYSEEPR
                     IIGKIIANFRPLEI"
BASE COUNT         4234 a         2155 c         2473 g         4017 t
ORIGIN      
        1 tattaatggt acgaagaaaa gttagttgct ctttccacaa gtatttttga ttagcgagtt
       61 taatttgctt tgtatttgtt gattgatagc aatagagaga tagcgctttt ttccagttat
      121 tattattcta tcagatttat ctaatgtaaa gtttctataa gagtctgatg tcgtgtaatc
      181 aatcactaaa tgagtagggg ttagttgata gtgaatatca gggcaatttt gttttaaaaa
      241 gagagccaat tgacgttccc gctgttttag ataacgctta ataaagcgaa gatttttttg
      301 aaacatccca ttttctaaat aaagagctaa tgctttttgc attagtaagt tcgtgtctaa
      361 atcaatcaat gatttttggg ttaaaaaatg aggttttaaa cctgacggta aaactagagc
      421 tccgatacgg agtgcaggaa aaacactcat tgaaaaagat tttagataga tgattcggtg
      481 atgagtgtca taataatgaa ttggtggctc tttaagtttc acaaaatctc ctaaataatc
      541 atcttctaaa atataaacct ggtatcgttg tgctagacga acaatagctt ccttttcctt
      601 agtgttataa gaaagtccca gaggatgtga aaatcgtgaa atcgtgtaaa aaaatttaat
      661 atcgccagtt tgaaataaag attctaagag ttctaaatca aggccattaa agtgtctaga
      721 gatagtttga tagggtaatc ctaactgagc aacaatagct tccatccgat gataagtagg
      781 cttttcaagg agaattgttt tgcccgcatt aggaaaaggc atttgtgata aaatataaag
      841 cgcttgttgc gtcccagagg tgattaatag ttggtctttg ttactgtaaa cactattttc
      901 agctaagtaa agaagcaaag cttcccttag ttcttctagt ccttcggttt tgtgataata
      961 atgaaagagg tatttgtctt tagcacttaa agcctcattt aagcagagtc ggaaatcttc
     1021 ataagccata ttattataat cttcaagact tagattaaga acattatcgg gtatgcttac
     1081 gttacccaaa acataataac cacttttagg aacagcataa attaagtgac gatattttaa
     1141 ttctaaaaga gcccgctgaa cagtatcttt gctacaatga taaaccttac ttaataccct
     1201 aattgaaggt agcttgtccc ctttttttag tcgctgtttc tggatatctt gttcgatatt
     1261 ggaaatgatt gtttgatatt ttgttgtcat aaattgtccc catacagtta agtgttataa
     1321 ctattgtatc ttatgcctct ttttcttaca atagttatta tagaaaagag gtaatagcta
     1381 tgaaacggat tattgtggca aatgatctgg ttggtgttgg taaggtagct ttgtctgcta
     1441 gtattccttt aatggcttct tgtttgttag agcaagtttt attaccaact tgccttttat
     1501 caagccatac tggcggagga cagagccctg tgaagcatac tacatcaagt atattagacg
     1561 tttttttaac agattgggac aacaaaagaa ttaagtttga cggcattttt ataggttatt
     1621 ttacgacaac aagagatggg caaaagctgc tagaatggct ttcaagaaag caacttccct
     1681 taattttaga tccaattatg gcagataatg gaaaacttta tcaaggcttc agtcaaaaac
     1741 atatagagat tatgataaaa cttactcaaa aagctgagct tgttttacct aatattagtg
     1801 aggcttgtct attaacacaa acagcttatt tagcaaaaaa ttatgatgaa gaagatatta
     1861 gacaggtggc tagaaaatta gctaagcttg gacctaaaca ggttgtcatt agcggaatct
     1921 cttttgagga gggcagcatt ggttttgctt actatcagtc agattcagat caattaacat
     1981 tccattttgg gaagcaattc ccttaccatt tctatggtac aggagatata gcatcagcta
     2041 ttataacagc aggattctat tatcagctac cactaacaca aattctagat cttgttgttt
     2101 catttcttga aaaagtattg caaaataccc ttacattaaa aagagattta atcttaggtg
     2161 ttgcttatga atctcattta aattacttaa ctaaatcgtt taaaaagttg ttggaggaaa
     2221 aaaatgtcat ctcaaaaatt agctaattat attgaattga gtatgtatgc cgctttaatt
     2281 tttatttcag tacaagtgtt gcgtgtccct gtcggtatgc agtttattca tcttggcaat
     2341 gcattagttg ttattgcagt cttaatctat ggtagtaaaa aaggagctct agttgctagt
     2401 cttggtttag ggatctttga tgttttgaac ggctatgctt cagtcgtttg gataactatc
     2461 ttagaatcct tggctatttg tttaattttg catttggtat atgaaagagc tatgcaatct
     2521 agtaaaaaac caaaagccat tataacaata acagttattg ctgcattgtc aaaattaact
     2581 attaacctta taaaatactt actgttagct tatataggta ctcagctacc actttcggta
     2641 tctttttggg tagctcttgg aaaaattggt ggtagtttcg gaacatcttt tgtgacttta
     2701 gttgctgttc ctcttttgta tcctgttatc aaaaacttaa aagaaaaggt tacaatcgga
     2761 caagcaaaaa gaggtttttg atacaagtac cttatcgata aaaaagcaag gtacactttt
     2821 agtcaaatat tgacaaaagg ttacaatttt gccataatga aataactata aagccgtttt
     2881 gacggctttt ttgatgacag ttaaggaaga aacgatgagt attttagaag ttaagcagtt
     2941 gagtcacggt tttggggatc gggctatttt tgaaaatgtg tcatttcgcc tcttaaaagg
     3001 cgaacatatt ggactagttg gggcaaatgg tgaaggaaaa tcaaccttta tgagtatagt
     3061 cacaggacat ttacagcctg acgaaggaaa agtagagtgg tcgaagtatg tcactgcagg
     3121 tcacctggat caacatacag tgttggaatc aggacaaacc gttcgtgatg tcttgcgaac
     3181 tgcttttgat gagttattta agaccgagaa tcgtattaat gagatttacg cgtcaatggc
     3241 agatgataaa gctgatattg ctgttttgat ggaagaagta ggtgagcttc aagatcgttt
     3301 agaaagtcgt gatttctata ctttggatgc taagattgat gaagtagcgc gtgcgcttgg
     3361 tgttatggat tttggaatgg agtcagatgt tacatcctta tcaggtgggc aacgaacaaa
     3421 ggttttatta gccaaattac tattagaaaa acctgatatt ctgctattag atgaaccaac
     3481 taaccatttg gatgctgagc atattgaatg gttaaaacgc tatttacaac attatgaaaa
     3541 tgcttttgtg ttgatttcgc atgatatttc tttcttaaat gatgtgatta atattgttta
     3601 tcatgttgaa aatcaaagtt tagttcgcta tactggggat tattaccaat ttcaagctgt
     3661 ttatgagatg aaacaatctc aacttgaagc agcctatgaa cgtcaacaaa aagagattgc
     3721 taacttgcag gattttgtca accgaaataa ggctcgggta gcgacacgta acatggcaat
     3781 gtctcgccaa aaaaaacttg ataagatgga tataattgaa cttcaagctg agaaaccaaa
     3841 accaaatttt gaatttaagc aagctagaac tcccagtcga ttcatttttc aaacaaaaaa
     3901 tcttgtgatt ggttatgatt acccattgac caaagaaccc ttaaatataa cgtttgaaag
     3961 aaaccaaaaa attgctattg ttggggccaa cggtattgga aaatctactt tgctaaaaag
     4021 tttattaggt gttatcgagc ctttagaagg tcatattgtc acaggggatt ttttagaagt
     4081 tggctacttt gaacaagaag tgacaggtgt taaccgacaa actccgctag aagtagtttg
     4141 ggatgctttt cctgccttaa atcaggcaga agttcgagcg gcactagctc gttgcggact
     4201 aacatcaaaa catattgaaa gtcaaattca agtactttcg ggtggtgaac aagcaaaagt
     4261 tcgtttttgt ttgttgatga atcgtgaaaa taacgtgctt attttagacg aaccaacaaa
     4321 tcatcttgat attgatgcta aaaatgagct caaacgtgct ttaaaagcat ataagggttc
     4381 tattttaatg gtttgtcatg aacctgattt ctacaatggg tgggtaaccg atacttggga
     4441 ttttagtaag ttaacctaaa ctattcctta attttttaca cagaaattac taaaatcagt
     4501 aaagatatag aataaatact gctagatatg tgatggttta ggtaactgat caccaaccaa
     4561 aaaagatgtg actacatctt tttttatcta atggaatggt gtaaagtcgt ttaataatag
     4621 cttatttttt gatgatcagt tgttcatgaa attgttggtg attaacaagt catttaagat
     4681 acaaacgtta ttactaaaaa gtgattcaaa tagtttccct atttttatgt ttttttaaca
     4741 tttattacga ataaatagat aaagacagtg aagatgatta gtaagtacat ctgctaagat
     4801 tacgaataga tggggacaat aaatgacgaa tttaacgttt tatgcaaaaa tcggtatttc
     4861 agaagaagag catgactctt ttgttaaaga acatcagcaa attagcgttt tacaaggtag
     4921 tgattgggca aaaatcaaaa atcaatggca gaatgagcga attggtatct ataaagagga
     4981 aaagcaggtt gcctctttat cacttttgat taagctattg ccacttggaa gaagcattat
     5041 ttatattcca agagggccag tcatggatta tcttgaccgt gatttggtgg catttaccat
     5101 gaaaacacta aaggattatg gtaaaactaa aaaggccctc tttatcaaat atgatccagc
     5161 tatcctgtta aaacaatacg cactgggaca ggaagaagaa gaaaaacctt tagctttagc
     5221 agctattaag aatctccaag aagctggtgt tcattggact ggtttaacaa tggagattgc
     5281 agatagtatc caacctcgtt tccaagctaa tatttacact caagaaaacc ttgagatgca
     5341 atttcctaag cataccagac gtttaataaa agatgctaag cagcgtggtg tagaaacata
     5401 tcgtgccagt caatcagaac ttcacaaatt ttccaagatt gtctccttaa cagaaaaacg
     5461 taaaaatatt tctttgcgta acgaagctta ctttcaaaag ttgatgacta cttatgggga
     5521 taaggcctac ttacatctag caaaagtgaa tattcctcaa aaactagatc aataccgcca
     5581 gcaattaatt cttattaacc aagatattac tcgcacccaa gctcatcaaa agaagcgttt
     5641 aaaaaaatta gaagatcaaa aagcttcttt agaacgttat ataactgaat ttgaaggctt
     5701 tacagaccaa tatcctgagg aagttgttgt agcaggtata ttatctattt cttatggaaa
     5761 tgttatggaa atgctttatg ctgggatgaa tgatgatttt aagaagtttt atcctcagta
     5821 tctgctgtat cctaatgttt ttcaggatgc ttatcaagat ggtattattt gggctaacat
     5881 gggaggagta gaaggctcgc ttgatgatgg acttaccaaa tttaaggcca actttgctcc
     5941 gacaatagaa gaatttatag gagaatttaa tctccctgtc agcccacttt atcatattgc
     6001 taataccatg tacaaaatac gaaaacagtt aaagaataaa cattaaaaga ctcgccaaaa
     6061 tgagcgagtc ttttaatgtt tattctgtta ttcccattca actgttgcgg gtggtttact
     6121 tgtgatgtcg tagacgatac ggttaacgtg gtcaacttca tttacgatac gtgttgagat
     6181 ttttttcaag acatcccaag gaagttgagc aaagtcagct gtcataccat caatagacgt
     6241 aatagcacga atggcgatgg tataatcata agtacgacca tctcccatga cgcctacaga
     6301 acggacacct gtgttaactg taaagtattg ccacacgtca cgatcaagtc cagccttagc
     6361 aatttcttca cgaaggatag cgtctgattc gcgaacggtt tcaagttttt cttcagtaat
     6421 tgctcccatg acacggatag caagtccagg acctggaaat ggttggcgcc aaacaatttc
     6481 ttcaggcatt ccaagagcga ttccaagcgc tcgaacttca tctttgaaaa gagtgtttaa
     6541 gggctcaatc aattcaaact gcatgtcttc ggggagacca cccacattgt gatgtgattt
     6601 gatggtttga gcagtttctg ttcctgactc aatgatatca gtataaagtg ttccttgggc
     6661 aaggaagtca acacctttta atttgctggc ttcatcatca aaaacataga caaattcatt
     6721 accaataatt ttacgttttt tctcaggatc ttcaacgtct gcaagaaggt ctaagaaacg
     6781 ttttgaagca tccacacgga taatatttag gccaaatttg cccccaagca ttcccataac
     6841 ttgatcgccc tcgtctttac gaagaagacc gtgatcaacg aaaatacaag ttaattggtc
     6901 accgatagct ttttgaagta gaacaccaac aactgaagaa tcaactccac cagaaagacc
     6961 tagaagaact ttacgatcgc ctacagtttc acgaatttta gcaatttcca tgtcaataaa
     7021 attatccatt gaccaatcac cacgcgcgcc acaaattgat atagcaaagt tttgaagaat
     7081 gtcatttcca taaacagagt gtcttacttc tgggtggaac tgaataccgt aaaggttttt
     7141 ctcagtattt tcaatagctg catagggaca gtcatttgag tctccaacaa ggtggaaacc
     7201 ttctggaatt tcagtaacag catcaccatg gctcattaaa acgagttgtt cttgaggtgt
     7261 gcctgaaaat aattttgacg tttcacgaag atgaagggtt gactgaccgt attcacgatt
     7321 accagcttgt ccagcaggaa caactttacc acctaattta tgggtgatta attgcatacc
     7381 gtaacagata ccaagaatcg gaatccctag ttcaaagatt tcagggtcaa tgccaaaggc
     7441 gttatcagcg taaacagagt taggccctcc tgataaaacg atacctatgg gattgatctc
     7501 acgaagttct tgagcggtga ttttatggct ttttagttcg gagaaaacac caaactctcg
     7561 aatacgtcta gcaataagct gattgtactg gctaccataa tcaagaacga taattttttg
     7621 aacatcattc aaaattgaaa tttcagtcat tataatcctt tcattctata ccaaccgtca
     7681 ggctgatagt tgtcaactta ttgtagcata ttttcatcga aaagacatta caatttctct
     7741 agctcgttgg aaaaaaacgt aatcgttatg ttacaataga ggctatgaaa gggaattaag
     7801 acaacaggga gggcctatgc taagaaaaaa gagaattttg ggtttatttc gacctgttga
     7861 attaattttc ctaggattat tgttgagttt agttgtttcg taccttgcgt ggaccaatag
     7921 ttttgcaaca cttcacaata tattagcaac tgtcggaata gtggaacgaa gcaaagatca
     7981 acagcctcgc tatcacattg gacaagctat tcaagtacaa aaaagtggtc catatcatca
     8041 atggattgga actattaata aacaggtaga agatatagcc gaaaattacc gagtgagtta
     8101 tcattatgaa gtggtatttc caataggaaa agtcactgtt tctttgccag aacataacct
     8161 gaaagagcct gataaaccga ggtttaaaaa aggagatatc gttaaattat cttcattaac
     8221 taaaaagcca catataaaag tatatcaagg tcaattagcg actattaaac aagttaaaaa
     8281 acgctatgac tattcgttag gaggatatca gtacgatatt aatctgaaag ataatctaag
     8341 attagatgga atttcagagc aagattttgt taaaccttat tatattaggt tcaataaagg
     8401 aaattcccct gagcaaaaca atcgtctttt gcgaaaagct ttcgcttatg caaagcagca
     8461 tccaaatagc gttatatctt ttccaaaggg acaatttcac attggctctt tgccttcaca
     8521 aaaagattat tttgagcttc catctgatac agctattatt ggtcatcaga cagagttcat
     8581 tattcacggt aaaatgttgt ggtttggatt ccctacagga ccaaaggctg aacaaggtgt
     8641 tcgtaatctc gtgttgactg gagtgcattt caaagcaaat gatttgaaaa aaggagacca
     8701 ctttatgatt atggctgatc atggtactga ttggcatatt tacgataaca aatttactat
     8761 ggttcataag cgtaatagtc atatttttga tttaggatct ctacaaaatt cattgtttga
     8821 gaaaaaccaa tttattggct atgcgccaga attagtacaa gaccaacagc tgctatcaaa
     8881 ggctcaaggg catgattttt tttcagaagt cattcagttt gatgctgctg ttcatcattt
     8941 tgcatgggat ggaggtctac ttagtaatat tgctccaaac tatgaagcat ttaaccaaac
     9001 tcgacatcta tgtcacaata ttactgtaag ccaaaatcaa tttttacctt atatagatcc
     9061 gactggttgc ctgagagcct atagtggttc tattggtcag cattcctcaa aagtaggagt
     9121 tattagggtt ttaaataatg tttttacctc atccattgtt actaaagcga agctcactag
     9181 ttggtttatg gaacctattc attttccacc aaattcacca gttattgtcg caggtaatat
     9241 cattaattga tgccaaatac ctcaatattt acaattcaat tttcatcaaa aatatgataa
     9301 aatagatgaa taaccgactt gaggagtgtg gcatgttacc agcttatata aaaattcatg
     9361 atgcaattaa aaaagacatt gatctgggca tctggccaat tggtagtcgc ttaccaagtg
     9421 aacgacattt agcggaacat ttcaccgtta gtcgaatgac tctacggcag gctattacat
     9481 tactagtaga agaagggatt cttgaaaggc ggataggaag tggtacttat gttgccagtc
     9541 atcgtgttca agaaaaaatg cgaggaacaa caagtttcac agaaattata cgctcccaag
     9601 gacgacaacc ctcctctaaa ttattatcct atcaaaagca attagctagt gataccgaag
     9661 ttaaagaatt gaacttagac aaaacagact tggtcattcg aatggaacgt atccgctatg
     9721 cagacagtgt ccctttggtg tacgaaattg cttcaatccc agaaaaattt ataaaaacgg
     9781 ttaaacgagc tgatattaca gaacactttt ttcactcatt gatagcaaat ggttacgaga
     9841 ttggtaaaag taaacagaca atctacgcta agctagcaag tgagcgtgta gcctcctact
     9901 tggaagttgc taaaggacat gccattttgg ctctaactca ggtgtcttac tttacagacg
     9961 gcaaaccttt tgagtatgtc cgcagtcagt atatcggtga ccgttttgaa ttttacttag
    10021 aaaataatta gtaaagaagt catttatgac ttcctttttt acacgaacag cataaggcaa
    10081 ttaatttgct actgttaaaa acaatcatag tatgataaaa tgaacattat ggaaattgaa
    10141 aaaacaaaca ggatgaatgc cctttttgaa ttttatgcag ctttgctaac agataaacaa
    10201 atgaattata ttgagctgta ttatgccgat gattatagct tagctgagat tgctgatgaa
    10261 tttggtgtca gtcgtcaggc tgtctatgat aatattaagc gtacggaaaa aattttagag
    10321 acttatgaga tgaaacttca tatgtattcg gattatgttg ttcgaagtga aatctttgac
    10381 gacatgattg ctcactaccc tcatgatgag tatttgcaag aaaaaatttc cattctaacc
    10441 agtattgaca atagagagta agaggagttt tgactatggc ttttgaaagc ttaacccaac
    10501 gtttgcaaga tgtgtttaaa cacattcgcg gtaaaaagaa attatcagaa tcagatgttc
    10561 aagaagtgac aaaagagatt cgtctagctc ttttagaagc cgatgtcgct ttaccagttg
    10621 ttaaaacctt tattaagcgc gtacgtgagc gcgctattgg ccatgagatt attgatactc
    10681 ttgatccaac acaacaaatt cttaagattg ttaatgaaga attaacgagt attttaggct
    10741 cagaaaccgc ggagattgat aaatctccaa aaattccaac tattatcatg atggtcggtt
    10801 tgcaaggggc tggtaaaacc acttttgcag gaaaactcgc taataaatta attaaagaag
    10861 aaaacgcgcg accactgatg attgctgccg atatttaccg tccagcggcc attgatcagt
    10921 taaaaacact ggggcaacaa atcaatgtac ctgtttttga tatggggaca gaccactcag
    10981 cagtcgatat tgtcagaaaa ggtttggaac aagctcgtga aaaccgcaat gactatgtct
    11041 tgattgatac tgcgggtcgc ttgcaaattg atgaaaaact gatggaagag ctgcgcgatg
    11101 tcaaggcttt agcacaacca gatgaaattc ttttggttgt agatagcatg attggtcaag
    11161 aagctgccaa tgtagcctac gagtttaatc atcagttgag catcacaggg gttgtgttaa
    11221 caaagattga tggtgatacc cgtggaggtg cagcactatc tgtccgtgaa atcactggaa
    11281 aaccaattaa atttacaggt attggtgaaa aaatcacaga tattgaaaca tttcacccag
    11341 accgcatgtc cagtcggatt ttgggaatgg gtgacttact cactcttatt gaaaaagcta
    11401 gccaggaata tgatgagaaa aaatccttag agttagctga aaaaatgcgt gagaacacct
    11461 ttgatttcaa tgatttcatt gaacagttag atcaagtgca aaatatgggg cctatggaag
    11521 acctcttgaa aatgattcca ggaatggctg gtaacccagc tttggccaat atcaaagttg
    11581 acgaaaacca aattgctcgc aaacgtgcta ttgtctcttc gatgaccccc gctgaacgtg
    11641 aaaaccctga cttactaaac ccgagtcgtc gccgtcgtat tgctgctggt tccgggaaca
    11701 gctttgtgga agttaataaa tttatcaaag actttaacca agccaaaagc atgatgcaag
    11761 gggttatgtc gggcgatatg tctaagatga tgaaagatat gggcattaat cccaataatc
    11821 ttcctaaaac catgcctgct ggtatgccag atatgtcttc cttagaaggg atgatgggac
    11881 aaggtggtat gcccgagtta tctggccttg gtggtgatat ggatatgagt caactatttg
    11941 gtaaagggtt caaggggaaa attggacaat ttgccatgaa acaggccatg aaacgtcaag
    12001 ctaacaaact aaaaaaagca aagaaaaaac gaaaataaaa gagacggaaa ttttcgtctc
    12061 tttttgatgt gaaattcatt tatcttgtat acaagattat taaaattatg gaagaccgcg
    12121 ttacatgcat gatgagttca tttaaaattt gaaataacct aatcaatcta acttaaattt
    12181 ctaagggcct aaaattagcg ataattttgc caataatgcg aggttcttcg ctatagggag
    12241 caaacttatc agaatatttt ttatttaagg acactagacg taatccctca tcttcacgaa
    12301 ataccttttt gatatatgtt tgcccatccc attcgactgc ataaattgct ccatcgtaat
    12361 caaaactatt ttgttttata agaacaactt cgccatttaa ataagttggc tccatagaat
    12421 ctccaaaaac ccaagacgca aaatcgtatt ctaattgttc atcgtaaaag acaacatcaa
    12481 agttaccatc accaaaatag gagtaaccag taccagcaga taaactttca tagacccgat
    12541 aagcatataa ttgtgatggt ttatctatga gatctttaga ttttttgtct atttgatgat
    12601 ttaacaatcg ctctgaatag ccaattactt tttctttatt agatattgtc agctggttat
    12661 aaggcgttag caatctatag tttgggtcga aataatcaga tgtcacattg aatagatgca
    12721 ataaggcatt taaatgtttt tcatttggta tattcttacc tttttcccaa ttagatattg
    12781 tcattttatt gacatgcaac aatcttccca attctgattg agttattccc tttgataatc
    12841 gtactgtttt tagttgatgt ccagaaaaca tttggtagc
//
LOCUS       DAUGHY010000041        12626 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.041, whole genome shotgun sequence.
ACCESSION   DAUGHY010000041 DAUGHY010000000
VERSION     DAUGHY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12626
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.041"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            15..542
                     /locus_tag="VH752_001297"
     CDS             15..542
                     /locus_tag="VH752_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEQ8289645.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
     gene            542..1660
                     /gene="yqeH"
                     /locus_tag="VH752_001298"
     CDS             542..1660
                     /gene="yqeH"
                     /locus_tag="VH752_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEQ8289646.1"
                     /translation="MEELFCIGCGIQIQTEDKEKAGFTPAAALKKGMETGELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIERINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGNKDVITTSRFPGTTLDKIEIPLDDGTFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNPEQTLFLGGLSRFDFINGERQ
                     GFTAFFDNQLELHRTKLAGADAFYDKHVGTLLTPPDKKELTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            1685..1993
                     /gene="yhbY"
                     /locus_tag="VH752_001299"
     CDS             1685..1993
                     /gene="yhbY"
                     /locus_tag="VH752_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEQ8289647.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            2062..2694
                     /locus_tag="VH752_001300"
     CDS             2062..2694
                     /locus_tag="VH752_001300"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEQ8289648.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLRMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDFYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            2691..3284
                     /gene="yqeK"
                     /locus_tag="VH752_001301"
     CDS             2691..3284
                     /gene="yqeK"
                     /locus_tag="VH752_001301"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEQ8289649.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLGVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECPDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLKDKDILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAKLDLNQA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEY"
     gene            3308..3661
                     /gene="rsfS"
                     /locus_tag="VH752_001302"
     CDS             3308..3661
                     /gene="rsfS"
                     /locus_tag="VH752_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEQ8289650.1"
                     /translation="MKKEELLKIVVEATDEKRAKDILALDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKETGGDASHVEGNSQAGWVLLDLTDVVVHLFLEDERYHYNLEK
                     LWHEAPAVALDAYLA"
     gene            3709..4452
                     /locus_tag="VH752_001303"
     CDS             3709..4452
                     /locus_tag="VH752_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ8289651.1"
                     /translation="MEHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKGRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLAIAKKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEVDVGDVFKEVYDVLANDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEVPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKLYADFEDKEPTETSKRWFFVAYK"
     gene            4674..5780
                     /locus_tag="VH752_001304"
     CDS             4674..5780
                     /locus_tag="VH752_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEQ8289652.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFVSVQSADYFAQGAIDILCQLGIQQLAFGTENVID
                     YQKLIKVYEKKSEQMTAYLSTLEDTLSYPQKTQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILTYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKNERLN"
     gene            6262..6978
                     /locus_tag="VH752_001305"
     CDS             6262..6978
                     /locus_tag="VH752_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ8289653.1"
                     /translation="MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            7181..8023
                     /locus_tag="VH752_001306"
     CDS             7181..8023
                     /locus_tag="VH752_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ8289654.1"
                     /translation="MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYRVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKL
                     PTAN"
     gene            8350..9195
                     /locus_tag="VH752_001307"
     CDS             8350..9195
                     /locus_tag="VH752_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ8289655.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGKAKYKSVADLPNGAQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            9450..10514
                     /locus_tag="VH752_001308"
     CDS             9450..10514
                     /locus_tag="VH752_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289656.1"
                     /translation="MNEAIIQLDHIDITFRQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFDQGKVQLSANALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEHKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGSVLDIFSNPKEALTQEFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPVGDMIVVLEGQAENIL
                     AAEKALHEAGVDVSILKRGA"
     gene            10515..11207
                     /locus_tag="VH752_001309"
     CDS             10515..11207
                     /locus_tag="VH752_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289657.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSVFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(11261..12595)
                     /gene="brnQ"
                     /locus_tag="VH752_001310"
     CDS             complement(11261..12595)
                     /gene="brnQ"
                     /locus_tag="VH752_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEQ8289658.1"
                     /translation="MLFALFFGAANLIYPAFLGIYSGHQILWSIIGFCLTGVSLPLLG
                     VIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGATSFSVGIAPILG
                     DNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISILVIASFVHPAGNY
                     GDAFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEATKQFGAKTDKEVT
                     KITLISGAIAILLLALVYIFVGRIGATSQSLFPFIDGSFTLHGNPVNGGQILSHASRF
                     YLGGIGQAFLAVVIFLACLTTSTGLITSSAEYFHKLVPALSHIAWATIFTLLSAFFYF
                     GGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIGLTFIPAIFDALL
                     TLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLILSKTKKS"
BASE COUNT         4145 a         2009 c         2657 g         3815 t
ORIGIN      
        1 attttggaga tgacatgagt atcgatgatt ataggccgac ctatatggta gaggctattt
       61 atgatttaag agcaaatgat ttgttgcgtc atggtattac ggctgttcta gtggatttag
      121 ataatacctt aattgcatgg aataatcctg atggcacacc agaggttcgc gcttggcttg
      181 atgaaatgac tatcgcggat attagcgtgg ttgttgtttc aaataataaa cattctcgtg
      241 ttgagcgtgc cgtttcacgc tttggtgttg actttattag ccgggctttg aagccatttg
      301 cttatggtat tgaaaaagcc attgctcgtt atggatttga tcgcaacgag gttatcatgg
      361 ttggagatca gctaatgact gatatcagag ctagtcatag agctggaata aaatcagttt
      421 tagtcaaacc acttgttgct tctgatgctt ggaatactaa aattaaccgc tggcgtgaac
      481 gccgtgtgat ggcaaaatta gaagagaaat atggtaaatt atcatatcaa aaaggaattt
      541 aatggaagaa ttattttgta taggctgtgg tatccagatt caaactgaag ataaagaaaa
      601 agctggtttt acgccagctg ctgctttgaa aaagggtatg gaaacaggag aactctattg
      661 tcagcgctgc tttagattga ggcattataa tgaaattaca gatgtccata ttaccgatga
      721 tgaattttta agacttcttc acgaagtggg tgatagtgat gctcttgtgg ttaatgtgat
      781 tgatattttt gactttaatg gttctattat tccaggtcta tctcgcttta tttctggtaa
      841 tgatgtctta ctggttggga ataaaaaaga tattttgcca aagtctgtta aggatggtaa
      901 agttactcaa tggttgacgg aacgtgcaca cgaagaaggt cttcgccctt tagacgttat
      961 gttaacaagc gctcaaaata aatatgcgat caaagacttg attgagcgta tcaatgagtt
     1021 acgtaatggt cgagatgttt atgttgtggg ggtcactaat gttggtaaat caaccttgat
     1081 taatgcgatt atccaagaga ttactggtaa caaggatgtc atcacgacgt ctcgttttcc
     1141 tgggacaact cttgacaaaa ttgaaattcc tttggatgat gggacgttca tttttgatac
     1201 accaggaatt attcataggc accagatggc tcattattta tcccctaaag agctaaaaat
     1261 agtgagtcct aagaaggaaa tcaagcctaa gacgtatcaa ttaaacccag aacaaactct
     1321 ctttttaggt ggtttgtctc gctttgactt tatcaatggg gagcgtcaag ggtttacagc
     1381 tttttttgac aatcaactag aattacatcg tacaaaatta gctggagcag atgcttttta
     1441 tgataagcat gttggaactt tgttaacccc gccagataag aaagagttga ctgcatttcc
     1501 taaattagtt cgtcatgaat ttacaattga tcagaaaatg gatattgttt tttctggtct
     1561 aggatggatt cgtgttaatg gtcaaaaaga ctcaaaagcg atagttgctg catgggctcc
     1621 tgaaggggtc gctgtcattg ttcgtaaagc gattatttaa aaataaaaaa gaagttggaa
     1681 aaaaatgctt acaagtaaac aaagagcttt cttaaaatct gaggctcata gcttaaaacc
     1741 gattgtacaa attggcaaaa atggattaaa tgaccacatt aagactagta ttcgtcaagc
     1801 tttggatgct agagaattga taaaggtcac tttgttgcaa aatactgatg aagatattca
     1861 tgaagtagca gagattttag aagaagaaat tgggtgtgat acggttttga aaattggtcg
     1921 tatcttgatt ctctataagg tttctgctaa gaaagaaaac cgcaagttgt cccctaaagt
     1981 taaagcaatc tagtcctttt atttgtatag ggactggttt tatgagacga ggactattgt
     2041 cgcgagaatt ttggagaaag tatggcatta gaattgttga cgccttttac gaaggtggaa
     2101 ttagaggaag aaaaaaaaga aagcaaccgc aaacagattg ggattttagg agggaatttt
     2161 aatcctattc acaatgcaca ccttgtggtt gcagatcaag ttcgccagca attaggatta
     2221 gatcaggtgc tattgatgcc tgaatgtaaa ccaccgcatg tggatgccaa agagactatt
     2281 gatgagaagc atcgtcttcg catgttggaa ttggctattg aagatgttga agggctagct
     2341 attgaaacct gtgaacttga acgtcaggga atcagctata cctatgatac gatgctttat
     2401 ttgactgagc agcatcctga tgttgatttt tattttatca tcggagctga tatggttgat
     2461 tatttgccaa agtggcaccg tattgatgaa ctggtcaaac tcgttcaatt tgttggagtt
     2521 caacgtccaa aatataaagc tggaacctct tacccggtca tttgggtaga tcttcctttg
     2581 atggatattt cttctagtat gattagggat tttattaaaa aaggtcgtca gcccaattat
     2641 ctcttaccta agcgggtttt agactacatt actcaagaag gtttgtatca atgacatatg
     2701 aagattatct tccttatagc aggaccgaac tgttagcaaa aattgcagag cagatgagcc
     2761 ctaaacggtt taaacatgta cttggcgtgg aaaaggcagc cttgtccttg gcagagtgtt
     2821 atggttgtaa cccagacaaa gcagggttag cagctctttt acatgattat gccaaggagt
     2881 gccctgacca ggtcttttta gacttaattg acaagtacca attatctcca gaattagcta
     2941 aatggaataa taatgtctgg catggtatgg ttggtattta taagattcaa gaagacctag
     3001 ggctaaaaga caaagatatc ttgcgagcta ttgaaattca caccgttggc gctgctgaga
     3061 tgaccttgct ggataaggtc ttatacgttg ctgattatat tgaagaaggt cgcatttttc
     3121 ctttggttga tgacgctaga aaaatcgcta agctcgattt gaatcaggct gttgcctatg
     3181 aaacagtgaa cactgtcgct tatttagcct caaaagcgca gcctattttc ccacaaactc
     3241 tggataccta taatgcgttt tgcagctatt taaaggaata ctaatggata atgatatgag
     3301 gaataaaatg aaaaaagaag aactcttaaa aattgttgtg gaagcaactg atgaaaagcg
     3361 tgctaaagat attttagcct tggatttaga ggggttaact agtttgaccg attattttgt
     3421 gattgcgagt gcaacaaata gtcgtcaatt agaggctatt gcggataata ttcgtgaaaa
     3481 ggtaaaagag actggtggag atgctagcca tgttgaaggt aatagccaag caggctgggt
     3541 cttgcttgat ttgaccgatg tggtggtaca tcttttttta gaagacgagc gttaccacta
     3601 taatcttgaa aaattgtggc atgaagcgcc tgctgtggct ctagatgcct atttagctta
     3661 atgttacaaa gagaccttgt caatacgaca gggtttttga ggatgataat ggaacataat
     3721 tatgaaaaat ttgcgtcagt ttatgatgct gtgatggatg attctctata tgacttgtgg
     3781 acagattttt cacttcgaca tttaccaaag tctaagggtc gaaatcgttt gttagagttg
     3841 gcctgtggaa ccggtattca gtcagttcgt tttgctcaag caggttttga tgtgactggt
     3901 ttagacttaa gtcaagacat gctggctatt gctaaaaagc gtgcgcaatc tgctaaaaag
     3961 aaaattgatt ttattcaagg taacatgctg gatttgtcac aggtggggca gtttgatttt
     4021 gtaacctgct attcggattc catttgctat atgcaagacg aagttgatgt tggcgatgtt
     4081 tttaaagaag tttatgatgt tttagcaaac gatggtatct ttatttttga tgtgcattca
     4141 acctatcaaa cggatgagtg tttccctggt tattcctatc atgagaatgc agatgatttt
     4201 gcaatggttt gggataccta tgctgacgag gttcctcact ctgttgttca cgaactcact
     4261 ttctttatcc aagaagacga tggacgtttt tctcgctttg atgaagtgca tgaggaacga
     4321 acgtatgagt tattgactta tgatattttg ttggaacaag cagggttcaa gtcatttaag
     4381 ctctatgcgg attttgagga taaggagccg actgagacta gcaagcgttg gttttttgtg
     4441 gcatataaat agaagaaaaa agtagccttt ttagctatag gatttaatga attatcttga
     4501 atgttactta tatgaaaaga gttcattagg cattaaatgc tattatcaaa gcttagaagt
     4561 attggagaca ttacgataaa gaaagcattt tctattatac gttgtgctta agcatttgat
     4621 ttaactctta ttagccctat aaaagattat ttagtacaat ttgaggagat tctatgactg
     4681 ttaccggaat tatagctgag tttaatccgt ttcacaatgg acacaaatat ttgctagaaa
     4741 cagcagaggg actaaaaatc attgctatgt caggaaattt tatgcaaaga ggagagcctg
     4801 ctcttattga taaatggatt cgatcagaga tggctcttaa aaatggtgcg gatattgttg
     4861 tggaattgcc ctttttcgtt tcagttcaat cagcagacta ttttgctcaa ggagctattg
     4921 atattctttg tcaattagga attcagcaat tagcatttgg gacagagaat gtgattgatt
     4981 atcaaaaact gattaaggtc tatgagaaaa aatcagagca aatgactgcc tatttatcga
     5041 cgcttgaaga tacacttagc tatccacaaa aaacacaaaa aatgtgggaa atctttgctg
     5101 gagttaaatt ttcagggcaa acgccaaatc atattttggg gttaagttac gcaaaggcta
     5161 gcgctggaaa gcatatacaa ctatgtccta tcaagcgtca aggggctgcc taccattcga
     5221 aagacaaaaa tcatctgcta gcttctgcaa gtgccattag acagcacctg aatgattggg
     5281 atttcataag tcattcagta ccaaatgcag gtttacttat aaacaatccc catatgtcat
     5341 gggaccatta tttttcattc ttaaaatatc aaattctgaa ccattctgat ttaactagta
     5401 tttttcaggt caatgatgaa ttggcaagtc gtattaaaaa agcgataaaa gttagtcaaa
     5461 atattgacca cctagttgac actgttgcta ccaaacgcta tacaaaagct cgtgtaagac
     5521 gtattttaac ctacattttg gttaatgcta aggaacctac tttacctaaa ggaattcata
     5581 ttctagggtt tacctcaaaa ggacaagcac atttaaaaaa attaaaaaaa agcaggcctc
     5641 tgattactag aattggagca gaaacttggg atgagatgac acaaaaagca gacagcatct
     5701 atcaacttgg gcatcaggat atcccagaac aaagttttgg aagaatacct attataataa
     5761 aaaatgagag gctcaattaa actctaattg agtttttttg ggggcttgac tctaatatta
     5821 cgttgatggt ttatacttca gttaatccaa tgaggaaatt ttataacaat aattaaaaaa
     5881 gtcagtcaaa agatatgctt tcgaagaaca ggctaacgtt tttacaagat tatatcagta
     5941 atcattttga caattgtagt aatggaatgt tgccaaaatt tagaatgttg tgtgttatga
     6001 atgacaagat tttaaaggaa tactaattaa acgggaggtc gtgaaccgga agctgttgtc
     6061 actcaagcgc caaaatctgt ctgtaacata atattgatgt atgggaacgc aattatttgg
     6121 tttttattgt gatgcttaaa acgattttat atttctattt taccaaagct tttcaaagtg
     6181 ctataataag ggagttagag tcaatattag taacgttaat gttgacaata ttcaataaaa
     6241 tactattaaa aggagacaaa aatgggacgt aaatgggcaa atattgttgc taaaaaaact
     6301 gctaaagatg gagcaacatc aaaggtctat gccaaatttg gtgttgaaat ttatgtggct
     6361 gccaaacaag gcgagccaga tccagagtta aatacagctc ttaaatttgt cattgatcgt
     6421 gcaaagcaag cacaagtgcc aaagcatgtt attgataaag cgattgataa agctaaagga
     6481 aacacagatg aaactttcgt agagggacgc tatgaaggtt ttggtccaaa tggttcaatg
     6541 attattgtgg atactttgac atcaaatgtt aaccgtacag cggcaaatgt acgtacggct
     6601 tacggtaaga acggtggcaa tatgggagct tcaggatctg tatcctactt atttgataaa
     6661 aaaggggtca tcgtttttgc tggtgatgat gctgacagtg tctttgaaca attacttgaa
     6721 gcggatgtag acgtagatga tgttgaagca gaagagggaa caataacagt ttataccgcc
     6781 ccaacagatc ttcataaagg tatccaagca cttcgcgata atggtgtaga agaattccaa
     6841 gttactgaac ttgaaatgat tcctcaatca gaagtagtat tggaaggtga tgaccttgaa
     6901 acttttgaaa agcttattga tgcactcgaa tcagatgatg atgttcaaaa agtgtatcat
     6961 aatgtagcag atttttaaat tgacatagaa taataaaaag agttgaaaac tatttcagct
     7021 ctttttaagt gatgctttta taagtaagta ccaagtaaca tagtcgttgt tagaagataa
     7081 tgaacaatag taaatcagta tcaacttgat aggaatcaat tattagtgaa ctctaattaa
     7141 aaatggtaaa ctagacacaa gaacaaaaag gagagatagg atgattaaaa aacaatattt
     7201 aggaagggca acaattgccc tagcatcaac ccttgtacta gcagcttgtg ggtcatcaaa
     7261 aacagctgag tcaggaaatc aggggagttc taaagaagtc ttatttgcaa cggttggaac
     7321 aacagctccc ttttcttatg aaaaaggtgg tcaactaact ggctacgata tcgaggtggc
     7381 taaagcagtc tttaaaggat cggatgatta cagggtgtcc tttaaaaaaa cagaatggtc
     7441 ttctatcttt acaggtttgg actcaggaaa ataccaaatg ggtggtaaca atatttcctt
     7501 taccaaagag cgttcagcta agtacctttt ttcttatcca atcggatcaa caccttctgt
     7561 tttagttgtt ccaaaagaca gtgatatcaa gagctttgat gatatccaag ggcatacgac
     7621 acaagttgtt caagggacta caagtgttgc acagttagaa gactttaata agaaacacag
     7681 tgacaacccg gttactctaa aattcaccaa tgagaacatt acccaaatgt taaccaattt
     7741 gagtgaaggc aaggcagatt tcaagatttt tgacgctcca acggttaacg ccattattaa
     7801 aaatcaaggg ttagataatc tcaaaacaat tgaactcacc tcaacagagc aaccatttat
     7861 ttattttatc tttagtcaag atcaagagaa attgcaaagc tttgttaaca agcgtattaa
     7921 agagttgact gctgacggaa ctcttagcaa gcttgccaaa gagcaccttg gcggtgatta
     7981 cgtgccatca gataaagagt tgaaacttcc tacagctaac taaaccatct attaaccagt
     8041 tattcatcta gaagactggt ttttctgatc ggaaacaata ttatagttta tgaggttatg
     8101 ttataaaaat tgcaaatagc cttaatcaaa atgagatgtt aaagtgcttt ttgagaagtg
     8161 aatattgtaa gaagtatgct ttgggtttta gtcctcaaat accaagtttt ttgttgacta
     8221 aacaaataag gatggttagc atagcatata gtttaaaact atgctatatg ctagccatta
     8281 tcaatttgaa aactatatct aattaagata caataatgat cgtaaacaaa atgaatcgag
     8341 gataacgcta tgtcttttaa aaaatggatt ggaatagtcg gaatcgcttt agcatcaact
     8401 gtattggttg cttgttctga aaaacaagat gataaaaaca ctctaactat tggggtgatg
     8461 acaaaaacag aatctgatca agcgagatgg gataaagttg aagaattatt gaaaaaagac
     8521 aatatcactt tgaagtacaa agaatttaca gattactcgc aacctaataa ggcagttgct
     8581 aatggtgaag tcgatattaa tgccttccaa cactataatt tcttaaataa ctggaataag
     8641 gaaaataagg aacatttggt ggccattgct gatacctata tcagcccaat taaccttttc
     8701 tctggaacca gtcaagatgg gaaggctaaa tacaaatcag tagctgatct gccaaatggt
     8761 gctcaaattg cagtaccaaa tgatgcgacc aatgaaagcc gtgccctcta tgtgctgcaa
     8821 tcagctggcc tcattaaatt gaatgtgtct ggtgatcaat tagcaaccat tgccaatatc
     8881 tcagagaaca aaaagaaatt agatatcaag gaattagatg ctagccaaac ggcacgtgct
     8941 ttagtatctg ctgatgcagc tgttgtcaat aatagttacg ctgttcctgc aaaaattgac
     9001 tacaaaacct ctcttttcaa agaaaaagca gatgacaatt caaaacaatg gattaacatt
     9061 attgcaggtc aaaaagattg ggaaaaatca gagaaggctg atgctattaa aaaactcatc
     9121 aaggcctacc aaactgatga ggtgaagaaa gtcgtggaaa aaacttcaaa tggtatagat
     9181 gtatccgtat ggtaaatttt agccattgtt ataacaagag tgtggtcgtg tttaaaacac
     9241 atgctttctt ttcttgcatg cttgaggaag agtttacgat taatttacct ttagaaagct
     9301 atttaagacc ttttcccttt taggatttag aaaccttggg aagactattg catgttatct
     9361 taatagtcat gattatgcag gcgatgaaaa cattgctatc gtagattact gtcgccatat
     9421 cgtactaata gaggagttat taaaatctta tgaatgaagc aataattcaa ttagatcata
     9481 ttgatattac cttccgtcaa aaaaaacggg ttatcgaagc tgttaaagat gtcacggttc
     9541 atatcaatca aggagatatt tatggcattg ttgggtattc aggtgccggg aaatcaactc
     9601 ttgtgcgtgt gattaacctg ctacaggcac caacaaacgg gaaaatcact gttgatgggg
     9661 atgtgacctt tgaccagggg aaagttcagt tatcagccaa tgcccttcgc caaaaacgac
     9721 gtgatattgg catgatcttc caacatttca atttgatggc ccaaaaaaca gccaaagaaa
     9781 atgtagcatt tgcgctccgt cattcgtcgt taagtaagac tgagaaagaa cacaaagtta
     9841 tcgaactgct agaacttgta ggtttatcag agcgagcaga taactaccct gcccagttat
     9901 cgggtgggca aaaacaaagg gtggctattg cgcgtgctct agctaacgat ccaaaaattt
     9961 tgatttcaga tgaggccaca tcagcactag atccaaaaac cactaaacaa attttggcgc
    10021 tcttgcaaga attaaaccgc aaacttggcc ttaccattgt tatgattact catgaaatgc
    10081 aaattgttaa ggatatttgt aatcgtgttg ctgttatgca aaatggtgtt ttgattgaag
    10141 aaggttctgt ccttgatatc ttttcaaatc caaaagaagc cttgacacag gaatttatca
    10201 caacagctac agggattgat gaggctttag aaaaaattaa ccaacaagac attgttaaac
    10261 atttgcctgc aaatgccctt ctagctcagt tgaaatatgc aggaacttct acggatgagc
    10321 ctctacttaa tagcatttac cgtcagtttg aagtgacagc taatattttg tatggcaata
    10381 ttgagatttt agatcatatt ccagttggtg acatgattgt tgtcttggaa gggcaagctg
    10441 aaaatattct tgcggctgag aaagccttgc atgaagctgg tgttgatgtt agtattttaa
    10501 agagaggagc ctagatgtca cagttaattc aaacttattt gccaaatgtt tatgaactcg
    10561 gatggtctgg agatgctggt tggggcttag cgatttggaa tactttatac atgaccattg
    10621 tgccttttat tgttgggggt gctattggtc tcttattagg cttgttatta gttttaacag
    10681 gaccggacgg tgttattgaa aacaaaacga tttgttgggt tattgataaa gttacctctg
    10741 tttttagagc cattcctttt gttattttga ttgctatttt agctagcttt acctatttgc
    10801 ttttaagaac aactttagga gcaacagcgg ccttggtacc tttaaccttt gcgacttttc
    10861 ctttttatgc ccgtcaggtc caagttgttt tttcagaatt agacaagggt gttattgaag
    10921 cagcacaagc ttcaggtgct actttctggg atattgtcaa agtgtatctc agtgaaggtt
    10981 taccagatct tattcgtgtt tctacagtca ccttaatttc tttagttggg gaaactgcca
    11041 tggcaggagc tatcggtgct ggaggattag gaaatgtagc gatttcttat ggttataatc
    11101 gttttaacaa tgatgtgact tgggtagcga ctattattat tcttttaatt atttttgcta
    11161 tccaatttat tggtgatagc ttgactagaa ggtttagtca taaataaacg aaacaacacc
    11221 ctaagtaaaa acttagggtg ttgttgttta gatgatgtca ttagcttttc ttcgttttac
    11281 ttagtataag ccctattaaa aaaccaatag cagcaaatat aatccatccc attgagaatt
    11341 gccctagtgg aacagttttt tggaaaaaag ttacaacggc ttctggtaaa tgaaataatc
    11401 cagtcatttg tgatagtgtt aggagtgcat caaatatggc aggaataaag gttagaccaa
    11461 ttgtagttcg atagacaata ggatcattat tgaagcattt ttgtgccaaa acaaggaaaa
    11521 ttaaatcgac tgttaatggg tataaaagga ataaaacagg agctgaccag ttgataatga
    11581 ctgataagcc accaaaataa aagaaagctg atagtaaagt aaagatagtt gcccaagcaa
    11641 tatgagataa agcaggcact aatttatgga agtattcagc acttgacgtg attaagcctg
    11701 ttgaagtggt tagacaggcc aggaaaatca caacagctag aaatgcttgt ccgatgccac
    11761 ctaggtaaaa acgagaagca tgacttaaga tttgaccgcc attaactgga ttaccatgaa
    11821 gggtaaagct gccatcaata aaaggaaata atgattgtga tgttgctcca atacgaccga
    11881 caaagatata gacaagtgct aatagcaaaa tggcaatagc cccagaaata agtgttattt
    11941 tggtcacttc tttgtccgtc ttagcgccaa attgtttggt agcctcaatg actaaaatag
    12001 caaaaacaag agaagctagt gcatccatag tgccataacc ttgaattaat cctgctataa
    12061 aaggaaaatc tttaaaggca ttattaacac cgaccccagc gttaaaagca tcaccgtaat
    12121 ttccagcagg atggacaaag gacgcgataa ccaaaataga aataacaact aacaacgttg
    12181 gcgttaaaaa ttttccgata ttttcagcta gcttactagg tttgatagca aggaagtagg
    12241 acagaccaaa aaatagtata gcataaatag ctttattggt tgtattatct cctaagatag
    12301 gagcgatacc gactgaaaaa gaagtggctc ctgttcttgg aatagcaaaa aatgggccaa
    12361 tagataaata caaaatggaa gaatagaaga ttgcatacca tttagagatg gggcgtgcca
    12421 aactttcaac atcgcctgat ccagatttag caacagcaat gacaccgagt aaaggcaagg
    12481 agacaccagt taaacaaaaa ccaatgatag accatagaat ctgatgacct gagtagatgc
    12541 ctaaaaaagc tgggtaaatg aggtttgctg ctccaaaaaa tagcgcaaat agcatgaatc
    12601 caatgacgat atatacattt ttttgt
//
LOCUS       DAUGHY010000042        12515 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.042, whole genome shotgun sequence.
ACCESSION   DAUGHY010000042 DAUGHY010000000
VERSION     DAUGHY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12515
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.042"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            29..550
                     /locus_tag="VH752_001311"
     CDS             29..550
                     /locus_tag="VH752_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ8289659.1"
                     /translation="MTKDRQIKKTKTAIYSAFIALLQKKEYSKITVRDMITLANVGRS
                     TFYAHYESKEMLLKELCEELFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSN
                     DPYFLLRFKNELEHDVYPNLRCKYIDKTTIPEVFLKQFVLSSFIETLKWWLHQRQRMS
                     ANELLKYYLEFIK"
     gene            765..1283
                     /gene="rimM"
                     /locus_tag="VH752_001312"
     CDS             765..1283
                     /gene="rimM"
                     /locus_tag="VH752_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEQ8289660.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DRFVQEVTIVSHRKQKHFDIIKFKDMYHINAIEKYKGYTLKVSKDNQGDLQEGEFYYH
                     QIIGMAVYEKDVLIGHVKEILQPGANDVWVVKRQGKRDLLLPYIPPVVLSVDVPNKRV
                     DVELMEGLDDED"
     gene            1273..1992
                     /gene="trmD"
                     /locus_tag="VH752_001313"
     CDS             1273..1992
                     /gene="trmD"
                     /locus_tag="VH752_001313"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEQ8289661.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTIVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLL
                     ERYDFSEEERKLLDKIKEALG"
     gene            2004..2996
                     /locus_tag="VH752_001314"
     CDS             2004..2996
                     /locus_tag="VH752_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ8289662.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGVELTENLIKQLSRFEDRTTICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEENFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLKTSTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEVLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGIFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            3174..4232
                     /locus_tag="VH752_001315"
     CDS             3174..4232
                     /locus_tag="VH752_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEQ8289663.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNRFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGNSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFEG"
     gene            4245..5168
                     /locus_tag="VH752_001316"
     CDS             4245..5168
                     /locus_tag="VH752_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEQ8289664.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     IVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPNNQDAGHQV
                     ADLLNKANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVELNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
     gene            5424..6137
                     /locus_tag="VH752_001317"
     CDS             5424..6137
                     /locus_tag="VH752_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ8289665.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKAIAQRASQLIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGVDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            6134..7045
                     /gene="pfkB"
                     /locus_tag="VH752_001318"
     CDS             6134..7045
                     /gene="pfkB"
                     /locus_tag="VH752_001318"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEQ8289666.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDIASTATGFLGGFTGRFIEESLSAEGVKTDFVKGDQDTRINVKIKSQEETELN
                     GQGPIISREQLEELKTKLSQLTAEDTVVFAGSAPANLGNAVYKELLPLVRQSGAQVVC
                     DFEGQTLIDALAYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNVIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            7042..8988
                     /locus_tag="VH752_001319"
     CDS             7042..8988
                     /locus_tag="VH752_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ8289667.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMTREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKAVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGHTYHAKNGKSET
                     AVSSEKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPKDQLGNLGSY
                     HEVAAIFMKIGGAAFSFMLPVLAGYIAYSIAEKPGLVAGFVAGTIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVVIGALVSGILFGALRKKA"
     gene            9081..9680
                     /locus_tag="VH752_001320"
     CDS             9081..9680
                     /locus_tag="VH752_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ8289668.1"
                     /translation="MKVKKRRRRARSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDAAFRSYGSLTACFLDYARVLNDPLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYHTKLNELIEWYQLTNFDGLMK"
     gene            9832..10539
                     /locus_tag="VH752_001321"
     CDS             9832..10539
                     /locus_tag="VH752_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ8289669.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNG
                     SSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
     gene            complement(10596..10907)
                     /locus_tag="VH752_001322"
                     /pseudo
     CDS             complement(10596..10907)
                     /locus_tag="VH752_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(11111..11476)
                     /locus_tag="VH752_001323"
     CDS             complement(11111..11476)
                     /locus_tag="VH752_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289670.1"
                     /translation="MPSTDDGILRTNTIAGNFFIDGQQQKTLINPIKIDKDIVTIQEF
                     DFKIRKFLMESKEIHLTKSPYIRGSLEIHSKNRKHEKINLYDAKPNSTRSDVLKKYKD
                     NKTINMKDFSHFDIYLWTK"
     gene            complement(11496..>12515)
                     /locus_tag="VH752_001324"
     CDS             complement(11496..>12515)
                     /locus_tag="VH752_001324"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HEQ8289671.1"
                     /translation="KRCYSTSAAVLAAVTLFALSVDRGVIADSFSANQEIRYSEVTPY
                     HVTSVWTKGVTPPAKFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNMLH
                     WWFDQNKEKIEAYLKKHPDKQKIMFGDQELLDVRKVINTKGDQTNSELFNYFRDKAFP
                     GLSARRIGVMPDLVLDMFINGYYLNVYKTQTTDVNRTYQEKDRRGGIFDAVFTRGDQS
                     KLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSNGN
                     LKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQDS
                     WNQTN"
BASE COUNT         3827 a         2078 c         2597 g         4013 t
ORIGIN      
        1 agtgaacaac atttagaaag tgtgtgatat gacaaaagac agacaaatca aaaaaacaaa
       61 aacagctatc tatagtgctt ttattgcttt attacaaaaa aaagaatatt cgaaaataac
      121 tgttcgagat atgattacac ttgctaatgt agggagatca accttttatg cacattatga
      181 aagcaaagaa atgttactaa aagaactctg tgaagaactt tttcatcatt tgtttaggca
      241 aaaaagaaat gtgacttttg aggactattt ggtacatatt ttaaagcatt ttgaacaaaa
      301 taaagacagc attgcgacgt tgttactttc taatgatccc tattttttat taagatttaa
      361 aaacgaacta gaacatgatg tttaccctaa cttgcgctgc aagtatatcg acaaaacgac
      421 tattccagaa gtattcctaa aacaatttgt tctatctagt tttatagaaa ccttaaaatg
      481 gtggctccat caaaggcaaa ggatgtctgc aaatgaacta ttaaagtatt atttagagtt
      541 cataaaataa tgactagtga gtcatcttgc caaattcaat tttttaatac agacggattt
      601 taacaattta gcagagaaag acaaaactgt aagtgcaaaa tagactataa cgtgataaaa
      661 taataggtaa tttggttctg aaaaagaagt gtctatcaaa ctttagaatc aagagaggtc
      721 gctaaagtga cgtgagataa agagttaacg atttaggagc taccatggaa tattttaatg
      781 ttggaaaaat tgtcaacacg caaggcctgc aaggagagat gcgcgtgttg tctgtcagtg
      841 attttgctga ggaacgcttt aaaaaaggct cacaattagc tttatttgat gataaagatc
      901 ggtttgttca agaagtgaca attgttagtc accgtaaaca aaagcatttt gatatcatca
      961 aatttaaaga catgtatcat attaatgcta ttgaaaagta caagggatac actttaaagg
     1021 tctcaaaaga caatcaaggg gatttacaag aaggagaatt ttattaccat cagattattg
     1081 gcatggctgt ttatgaaaag gatgtcctta ttggacatgt taaggaaatc ttgcagccag
     1141 gtgctaatga tgtttgggta gtgaaacgcc aaggaaaacg agacttgctc ttaccatata
     1201 ttcccccagt agttcttagc gtagatgttc ctaacaagcg cgtagatgtt gaattgatgg
     1261 aaggcttaga cgatgaagat tgatatttta accctttttc ctgaaatgtt tgccccccta
     1321 gaacattcca ttgtaggcaa agcaaaagaa aaaggattac ttgacattca ctaccataac
     1381 tttcgagatt atgctgaaaa agctcgtcat gtggatgacg aaccctatgg tgggggccaa
     1441 ggcatgttat tgagagcgca gcccattttt gacaccattg aacagattga agccaaaaaa
     1501 ccaaggatta ttcttttaga tccagcaggt aaacccttta ctcaggctta tgctgaagaa
     1561 ttagctcttg aggaagagtt aatttttatt tgtggtcatt atgaaggtta tgatgagcgg
     1621 attaagacct tggtgacgga tgaaatttca ttaggtgact ttgtattaac aggaggtgag
     1681 ttggcggcca tgactatagt ggatgcgacc gtcaggttaa ttccacaggt acttgggaaa
     1741 gaaagtagcc atcaggacga ttctttttca tcaggacttt tagaataccc tcaatacaca
     1801 agaccttatg attatcgtgg tatgacagtt ccagatgttc taatgagtgg tcatcacgaa
     1861 cgtattcgct tgtggcgtct agaagaaagt ctcagaaaaa cctatttgag aaggccagat
     1921 ttgttagagc ggtacgattt ttccgaggaa gagcgcaagc tattggataa gattaaagaa
     1981 gcattgggtt aaggagaaga ttagtgaaag ataaagcata tgatattacg attattggtg
     2041 gtggtccaat aggtttattt gcagcctttt atgcaggtct taggggcgta acagtaaaga
     2101 ttattgagag tttgtcagaa cttggtggac agcctgccat tctttatcct gagaaaatga
     2161 tttatgatat tccagcttat ccatctttaa caggtgttga gttgacagaa aatttaataa
     2221 aacaattaag ccgttttgaa gatcgtacaa ccatttgttt aaaagaagaa gtattaacct
     2281 ttgacaaggt taaaggtggt ttttcgatta ggactaataa ggcagagcat ttttcaaaag
     2341 ccattatcat tgcttgtgga aatggcgctt ttgcaccaag aactttaggg ttagaaagtg
     2401 aggaaaactt tgctgatcat aacctttttt ataatgttca ccagttggac cagtttgcgg
     2461 gacaaaaagt tgtgatttgt ggtggtggag attcagcagt tgattgggct ttggctttag
     2521 aagatattgc tgagagtgtg acggttgtgc accgtcgtga tgctttcaga gcacatgaac
     2581 acagtgtgga attattgaaa acatcaacgg taaacctctt aacaccttat gtgccaaaag
     2641 cattgaaagg aattggaaac ctggctgaaa aattagttat tcaaaaagtg aaagaagatg
     2701 aagtgttaga acttgaattg gacagtctta ttgttagttt tggtttttca acttctaata
     2761 agaatttgaa aaattggaat ctcgactata agcgttcaag tattactgtt tcaccacttt
     2821 ttcaaacaag tcaagaaggt atttttgcca ttggtgatgc agccgcttat aatgggaaag
     2881 tggatttgat tgctactggt tttggcgaag caccaacagc agttaatcag gccattaact
     2941 atatttatcc agatcgagat aaccgtgtgg tgcattcaac ctctttaatt gactaaggga
     3001 gtcggaagtt tcaagagcag tagcaacagt taatattcag tttgtcagga gaggacaaat
     3061 ggtttttacc taagtcttcg tgacaagttt tatactttgt gataaaatag tcattgtgaa
     3121 aaaagtgtga tggttttcac agattaacac attacaatga aaaggggtct atcatgacaa
     3181 caacagataa agagacattt agttctttta tgaataaggt cttggctggt acagcgattg
     3241 ctatcgttgt ggcactcatt ccaaatgcta ttttagcgac tttcttaaaa ccgcttttgc
     3301 caaacatggc agctgctgaa tttttacaca ttgtgcaagt cttccaattc tttacgccga
     3361 ttatggctgg tttcttgatt gggcaacaat ttaagtttaa tcctatgcaa cagttggctg
     3421 ttggtggagc tgcttatatc ggttctgggg cttgggctta tacagaagtc attcaaaaag
     3481 gtgtggcgac aggaacattc caacttagag gtattgggga cttaatcaat atgatgatta
     3541 cagctagcct tgcggtttta gctgtgaaat attttgggaa tagatttggc tctttaacca
     3601 ttatcttgtt acctatcact ataggaactg gcgtaggtta tattggttgg aaatttttgc
     3661 cttatgtgtc ttacgtgaca accctgatcg gacaagggat taattctttt acaaccttac
     3721 aacctatttt aatgtctatt ttaattgcag ttgctttctc gcttatcatt gttagtccga
     3781 tttcaactgt tgcaatcggt ttagctattg ggcttaatgg aatggctgcg ggagcagctt
     3841 ctatgggaat tgcatcgaca gcagcagtct tagtgtgggc gacgcttaaa gttaataaat
     3901 ctggtgtacc aattgctatc gcacttgggg ctatgaaaat gatgatgcca aacttcttga
     3961 aacatcctat catggctatt ccgatggtgt tcactgcagc aattagctca ttaacagtac
     4021 cattgtttaa tcttgttgga acaccagctt catctggttt tggcttagtt ggtgcagtag
     4081 gtccgattgc ttctttagca ggtggtaact caatacttat tattatcctt gcttggatca
     4141 ttgttccgtt tgcggttgct tttgcggcgc ataaggtttc taaagatatt ctaaaacttt
     4201 ataaagaaga cattttcgtc tttgaaggct aataggagga aaatatgtta gtttatattg
     4261 ctggctcagg agccatgggg tgccgttttg gttaccaaat ttcaaaaaca aataacgatg
     4321 tgattttgtt agataactgg gaagatcata tcaatgccat taaagaaaac gggttgattg
     4381 taacaggtga tgtggaagaa acagttaagc ttcctattat gaaaccgact gaagccactc
     4441 aagaagccga tttgattatc ttgtttacaa aagcaatgca gttgccacaa atgcttcaag
     4501 acattaaagg cattattgga aaagaaacca aagtgctttg ccttttaaat ggtcttggac
     4561 acgaagacgt tattcgtcag tacattccag agcacaacat tttgatgggg gtcactgtct
     4621 ggactgctgg tctagaagga ccaggtcgtg ctcatcttca aggagttggt gctcttaact
     4681 tacaaagtat ggatccaaac aatcaagatg cgggacatca agtagctgac ttgctaaata
     4741 aagctaactt aaatgcgact tatgatgaga atgttgtgcc aaacatctgg cgaaaagctt
     4801 gcgtgaatgg taccatgaat tcgacttgtg ctcttttaga ttgtacgatt ggcgaattat
     4861 ttgccagtga agatggtctc aaaatggtaa aagaaatcat ccatgagttt gttatcgttg
     4921 gtcaagcaga aggtgtcgaa ctaaacgaag aagaaatcac acaatatgta atggatacat
     4981 ctgttaaagc tgctcaccat tacccatcaa tgcaccaaga ccttgtgcaa aatcaccgat
     5041 tgactgaaat tgattttatc aatggtgcag tcaataccaa aggtgaaaag cttggtatca
     5101 atactccata ctgccgtatg attacagaac tagttcacgc taaagaagca gtgttgaaca
     5161 tccaataaac gctatcaaat cgttatactc aatattatgg ctaaagcttt aatattgagt
     5221 tttttgttat gctgatagta tttttcttag gcgtcattat ttgtgagcct agattatgag
     5281 ttaataaaag aaataataat tggtagaatt tgatagattt tgaaaggtta tgcttgactt
     5341 ttagttataa aatagtataa taattgtaaa cgatttcagt taggggtggt tctatcttaa
     5401 aatcaaaacg aaaacaatta atcatggcaa agatcactga agaaaactat gtttctttag
     5461 aggatttaat gcaattactc aactcctctg agtctactat ccgtagagat ctaggagagt
     5521 tggagcaaga aggacggcta catcgtgttc atggaggtgc tgaacttttt cactctttac
     5581 aagaggaact ttctaaccaa gaaaagtctg tcaaaaacag tcatattaaa aaagctattg
     5641 cgcaaagagc ctctcagctt atctatgaca acgatgtgat ctttattgat gctgggacaa
     5701 cgactgaatt tttattacct tttttacaag ctaaaaattt aactgtagtt actaattcta
     5761 ttcatcatgc tgctagatta gttgaactct ctattgagac gattattgta ggtggttatg
     5821 tcaaacaaac gaccgatgcc agtattggta atgtagcttt ggaacagatt aggcagatga
     5881 attttgacaa ggcatttctt ggaatgaatg gtgttgatga ttcttacctg acgactcctg
     5941 atatggaaga agcagttatt aaaaaagcgg ttctgtcaaa tgcaaaatta gcttatattt
     6001 tagtagatgg cactaaaatt ggtcaggtat cgtttgtaaa agtagctcct attaatgatg
     6061 tgactattat tacattagga ggatcagcaa gtattcttaa acaaataaag gaaaaggcaa
     6121 aggtgattga attatgattt ataccgtgac cttaaaccct tctattgact ttattgtcag
     6181 aattgaccaa ataaatcttg ggtcagtgaa tcgcatggcg agtgatgata agtttgctgg
     6241 aggtaaaggc attaatgtga gtcgtatctt gcaacgttta gacattgcta gcacggcgac
     6301 agggttcttg ggtggtttta caggacgttt tatcgaagaa agtttgagtg cagaaggcgt
     6361 taaaactgat tttgtaaaag gtgatcaaga tactcgaatc aatgttaaaa tcaagtcaca
     6421 agaagagacc gaattaaatg gtcaaggacc tatcattagt cgggaacaac tagaggagtt
     6481 gaaaactaag ctttcccagc taacagcaga ggatacagtt gtctttgcag gttcagcgcc
     6541 agctaatctt ggcaatgctg tttataagga attacttcct ttagttaggc aaagcggtgc
     6601 tcaggtggtt tgtgattttg aaggacagac tttaatagat gcgcttgcat ataatcctct
     6661 gttggttaaa cctaataatc atgaactaga agctattttt ggtacgattc tgactagtct
     6721 tgatgatgtt gaaacctatg cccgtcgtct tttagagatg ggtgctcaaa atgtcatcat
     6781 ttcaatggct ggtgatggtg ctttgctggt tactaaagaa gcgacttatt ttgctaaacc
     6841 gattaaggga gaagttaaaa attctgtcgg tgctggtgat tcgatggttg ctggctttac
     6901 aggcgaattt gtgaagagtc aaaatccaat agaagcctta aaatggggcg tggcttgtgg
     6961 tacagcgacg gctttctctg atgacttggc gacaattgca tttattaagg aaacatatca
     7021 taaagttgag gtagaaaaac gatgaaaatt caagatttat tgagaaaaga tattatgatt
     7081 ctcgatttac aggctatttc taaagaagtt gccattgacg agatgattac taaattagtt
     7141 gaaaaagata ttgtacatga ttttgatgtc tttaaaaaga gtatcatgac acgtgaagaa
     7201 caaacatcaa cagggcttgg tgatgggatt gccatgcctc attctaaaaa tattgtagta
     7261 gataaaccag cggtcttgtt tgcgaaatca aataaaggtg tggattataa ggctttagat
     7321 ggtcaaccaa cggatctctt ctttatgatt gctgcgcctc aaggagcaaa tgatactcac
     7381 ttggctgcgc tagcagaatt atcacaatac cttttgaaag acggctttgc tgataaactt
     7441 cgtgctgcag cgacaccaga agcagtcatt gctgtttttg atgaagcgtc aacagctaaa
     7501 gaagaagtgg ttgctccaac aagtggtcaa gactttatcg ttgctgttac agcttgtccg
     7561 acaggaattg ctcacactta tatggcagaa gaggctttga aaaaacaagc tgcagaaatg
     7621 ggagtagcta ttaaagttga aacaaacggt gcttctggtg tggctaatcg tttaaccgct
     7681 gaagatattc agagagctaa aggagttatt gttgcagctg ataaagcggt tgaaatggac
     7741 cgttttgatg gtaaacaatt cattgctcgc cctgttgcag atggtatcaa gaagagtcaa
     7801 gaattgattt ctttgatttt gaataatgaa ggacacactt accacgctaa gaacggaaag
     7861 tctgaaacag cagtatcatc tgagaaaaca agcttaggcg gtgctttcta taaacacttg
     7921 atgggtggtg tctctcaaat gttgccattc gttattggtg gcgggattat gattgctcta
     7981 gctttcttgt tggataacat gcttggtgtg ccaaaagacc aactaggcaa tcttggatct
     8041 tatcatgaag tagctgctat tttcatgaaa attggtggtg cagccttctc cttcatgtta
     8101 ccagtattag cgggttacat tgcatactct atcgctgaaa aaccaggttt ggttgctggt
     8161 tttgtggcag gtaccattgc ctcaaatggt cttgcttttg gtaaggtacc atttgcagca
     8221 ggaggagaag tgagcttggg cttaacgggc gtgccatcag gtttccttgg ggcgcttgtg
     8281 ggtggtttcc ttgccggtgg tgttattctt gcccttcgta aattgttggc aggcctacca
     8341 cgttctctag aaggggttaa atctatcctc ctttacccat tacttggcgt ccttgtcact
     8401 ggtttcttga tgctttttgt caatattcca atggcagcta ttaacacagc ccttaatgat
     8461 ttcttacaag gtctttcagg aagctctgct gtccttatgg gacttcttgt tggtggaatg
     8521 atggctgttg acatgggtgg tcctgttaat aaggcagctt atgtttttgg tacaggaact
     8581 ttagcagcta ctgtcgcaaa tggcggttca gtcgttatgg ctgctgttat ggcaggtggt
     8641 atggtacctc ctctagctgt ttttgtagcc actctcttgt ttaaagataa atttaccaaa
     8701 gaagaacgtg aatcaggttt gaccaacatt gtcatgggac tttctttcat caccgaagga
     8761 gcaattccgt ttggtgcagc tgacccagca cgtgcgattc ctagctttat cgcaggttct
     8821 gccttgacag gagctcttgt tggtttggct ggtattaaat taatggcgcc acatggtggt
     8881 atcttcgtaa tcgctttaac aagcaatcca atcttatatc ttgtctttgt tgtgattggc
     8941 gcccttgtat caggtatctt atttggcgct cttcgcaaaa aagcctaaaa ggtaaagaac
     9001 ttttttctta ctaaaactag aatctgtcaa ggttctagtt ttttgctata ctagagttaa
     9061 aggataagaa acgaggacct ttgaaggtga aaaaaaggag aagaagggct agatcatcag
     9121 taaatcgact tgtcttaggg ttagtattac ttaatctgat tgttagtatg tggacactaa
     9181 aattgggaaa ccagcgatta gctccgtatg cggatcacga aactctgaca tttgtcagga
     9241 agattagtca tgctgctcaa tcagtcgctc aaaagaaaca gttatacagt tcggtgatga
     9301 tggctcaggc cattttagaa tccaataatg gcaagtcaca actaagtcaa aaaccttatt
     9361 ataatttttt cgggattaag gggagctata aagaacggtc agtcattttt ccaactttag
     9421 aagacgatgg acaagggaat ctttatcaaa ttgatgctgc ttttcgttcc tatgggagcc
     9481 tgacagcttg ttttttagat tacgcaagag ttttgaatga tccactttat gacaaaaccc
     9541 acaaaaagtt ttggtctcat tatcaagatg ctactgcaac cttaacaggc acttacgcca
     9601 ctgatacaac ttaccatacc aaattaaatg aattgattga atggtatcaa ctcactaatt
     9661 ttgatggtct aatgaaatag ttatccacaa ttttaaaaaa aatcagaaga aatatgtcga
     9721 tttcaagaca aaagttatca atctcattaa attatctgtg gaaaagttaa tttaaacgtt
     9781 cgtgtgctat aatagaaaca tagaaaatgt ataaaaaagg atatgtgaat aatgacaaaa
     9841 aagaaaggta agcttgtact tatcagtctt tttgttctag cagcttgttt aggagcttat
     9901 agtgcaatga gacaatctca taaaacttca aacgtgtcag ctgagactat cgccagttct
     9961 tcaacccgac attttattga tgaaattggt ccaactgcta gtactattgg tcaagaacgt
    10021 gatctttatg cgtctgttat gatagcacaa gcgattttag aatcaagtaa tggtaaatca
    10081 agtctaagtc aagcacctta ttataatttt ttcggtatta agggtgccta taacggttct
    10141 tctgtgacga tgtcaacttg ggaagatgat ggtaatggca atacctatac gattgatcaa
    10201 gcctttagag cttatccaag tattgcagac tcccttaatg actatgcaga cttgctaagt
    10261 tctagcactt atataggtgc tagaaaatca aacacgctat cgtatcaaga tgctacagca
    10321 gctctaactg gcttatacgc tacagacacc agttataact taaaactaaa taatattatt
    10381 gcaacctatg gtttgacggc ttatgatgtt gctaatagct cagctcaaga aactggtcta
    10441 gcaacttcgg gttatgtttg gaatgagtat cgtcgtaatt acactgatgc ggagacccta
    10501 gcagttgatg aggcttgggc taaacgcatg acttattaat gtaacactaa caacaatctg
    10561 cttgcactga ccccaaaaaa gctggatact ctattttaag taaaggattt agttctgtat
    10621 tgtacagaac taaatccttt tagttttact ttgatatgtt tatcgttgta ataatcaatg
    10681 tcatttgtaa tcgcttgttc taagtcttct agtgatttat agttattctc ataaccatag
    10741 aacatttctg actttaagat gccaaagaaa gattccatca tgctattgtt aggactgttt
    10801 cttttacgtg acatagacgg acgaattcct ttagagttta ggaaatgatg ataatagttg
    10861 tgttgatatt gccaaccttg atcactctat catatagttc catcatagca tttatttgga
    10921 gatgtctttt tccattattg gtttaatgtg atttaagcaa ggttattagc taaacttaat
    10981 gccagacaaa aacaccccaa ttattagatt ttttgtctaa cttttggggc gcaattcaat
    11041 ctgagcagtt ttagatttgg tatcaatgtt attataggtc aattgatcat atttatccaa
    11101 agcttttagc ttatttagtc caaagataaa tatcaaaatg gctgaaatct ttcatgttta
    11161 tagtcttatt gtctttatat ttttttaaga catcagatct ggtgctattg ggttttgcgt
    11221 catataaatt gattttttca tgtttcctat ttttactgtg aatttctaaa ctccccctta
    11281 tataaggaga cttagtcaaa tgaatttctt ttgattccat caaaaatttt cttattttaa
    11341 aatcgaactc ttgaatcgta acaatatcct tatcgatttt tataggattt attaatgttt
    11401 tttgctgttg cccatctata aaaaaattcc ctgcaatagt attagtcctt agaataccat
    11461 catctgttga aggtattctt tcttattgat acgctttaat tggtctgatt ccaactatct
    11521 tgccctgttg aaagtgtaaa taaccctagt acttgagcac caatattatc ttcttttatt
    11581 tctttagcag aaatagctac ttttccagcg gaattaacac caacaaagta tttcttcata
    11641 ccaatagatg cattactatc agagtctgtt acataaatag ctttaaggtt cccgttagaa
    11701 tcaaagtcag ctccccacag gtttataaca tggttgatgc gtacgttagc gtaggtgtgt
    11761 gataggccta gagccttgcc ttcggttaac tctttcttaa tgagatcact gatttctttg
    11821 agattttttt ctttaaaatc atgacgactt gtcaatagct tactttgatc acctcttgta
    11881 aatacggcgt caaaaatacc acctcggcga tctttctctt gataggttct attgacatca
    11941 gtagtctgtg tcttataaac atttaagtaa taaccattga taaacatatc taaaacaaga
    12001 tcaggcataa ctccaattcg gcgtgctgac aaaccgggga aagctttatc tcggaaataa
    12061 ttaaaaagct cgctatttgt ttggtcacct ttggtattaa taacttttct tacatccaat
    12121 aattcttgat caccaaacat gattttttgt ttatctgggt gtttttttag atatgcttca
    12181 attttttctt tgttttgatc gaaccaccag tgaagcatat tccctgctgt ggcagccccg
    12241 caaagaagat cgtcttttcc attgaatgtt ttggtaatat cataccatcc ttggttagca
    12301 acataaggag cgtgaaaaac atcttcgcct tgagtgaatt ttgctggagg agtaactcct
    12361 ttggtccaaa cggaagtaac gtgataaggt gttacttccg aatatctaat ctcttgatta
    12421 gcagaaaaac tatctgctat aacaccacga tctaccgata gagcaaataa agtcactgct
    12481 gccaatactg cagctgaagt tgaatagcat ctttt
//
LOCUS       DAUGHY010000043        11762 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.043, whole genome shotgun sequence.
ACCESSION   DAUGHY010000043 DAUGHY010000000
VERSION     DAUGHY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11762
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.043"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..840)
                     /locus_tag="VH752_001325"
     CDS             complement(<1..840)
                     /locus_tag="VH752_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ8289672.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRMVQLLSFYDIKVPDDMSIITF
                     NNSN"
     gene            1084..2331
                     /locus_tag="VH752_001326"
     CDS             1084..2331
                     /locus_tag="VH752_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ8289673.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALIGAQYKGKTYAFPFGIESQVLFYNKSKLAAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKESDYLTKLQQFDKDIAATK"
     gene            2605..3966
                     /locus_tag="VH752_001327"
     CDS             2605..3966
                     /locus_tag="VH752_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ8289674.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFAYIYWCNLKSARNLYLFKQKGQKIPSFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINTKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIDLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            3966..4802
                     /locus_tag="VH752_001328"
     CDS             3966..4802
                     /locus_tag="VH752_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ8289675.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
     gene            4989..5763
                     /locus_tag="VH752_001329"
                     /pseudo
     CDS             4989..5763
                     /locus_tag="VH752_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(6277..7260)
                     /locus_tag="VH752_001330"
     CDS             complement(6277..7260)
                     /locus_tag="VH752_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ8289676.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGMQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKVVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTAPQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDYEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIAELVLKTVEHT"
     gene            complement(7291..8541)
                     /gene="dltD"
                     /locus_tag="VH752_001331"
     CDS             complement(7291..8541)
                     /gene="dltD"
                     /locus_tag="VH752_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEQ8289677.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTAQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(8534..8773)
                     /gene="dltC"
                     /locus_tag="VH752_001332"
     CDS             complement(8534..8773)
                     /gene="dltC"
                     /locus_tag="VH752_001332"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEQ8289678.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(8791..10047)
                     /gene="dltB"
                     /locus_tag="VH752_001333"
     CDS             complement(8791..10047)
                     /gene="dltB"
                     /locus_tag="VH752_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEQ8289679.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYREKANSAGVFYLAIAMAL
                     FPLIWVKVAPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRRFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKALMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNLP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(10044..11582)
                     /gene="dltA"
                     /locus_tag="VH752_001334"
     CDS             complement(10044..11582)
                     /gene="dltA"
                     /locus_tag="VH752_001334"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEQ8289680.1"
                     /translation="MIKDMIDSIEQFAQTQADFPVYDCLGERRTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQAKMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELSSGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(11594..11737)
                     /locus_tag="VH752_001335"
     CDS             complement(11594..11737)
                     /locus_tag="VH752_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEQ8289681.1"
                     /translation="MIKNKSRMRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIY
                     NEF"
BASE COUNT         3801 a         2345 c         1985 g         3631 t
ORIGIN      
        1 attagaatta ttaaaagtga taattgacat atcatctggc acttttatat cataaaaaga
       61 taataactgt accatacgaa tagccagaac atcaccaaca acaaccaagg cagttgcttt
      121 aaaggaagat atagtttcca tcaatatttc tacgctaaca ggatccttac gatcaaataa
      181 cagcattggc ttcgttttta aagatcgctt aaaacaaccc ttcaaatagc caagataacg
      241 ctcagaagtt acttctgata gtaaatcatc tgttataaat aatatgttgt gatggccttt
      301 ttgataaagg tgttctactg ctgttttagc cattagctga ttatcattat caatataagt
      361 ggttttatta gtgtcacctt gtggagcacc tacaatgaca aatggtaagt tatttgtcat
      421 caaatatttt ttgacaggat catcttgatc agaataaaga ataataaaac catcaactcg
      481 tttttgaaga tgcatcagtt tcacttgctc tatcaggtca tcaagagata tacctgtagc
      541 aatcgacaca gtaaaatgag attctttagc ttcgttagta atagtcgaca aaatttccat
      601 aaagaaaggt tcacttaatc tatcgctagt tgtaatagga ggaaaaacaa gtcctatatt
      661 gtgtgtcaaa ccgctcgcta gaatctgtgc tgcgacattt ggaacatatc ccaaatctgc
      721 catcgctttt ctaaccttct cttttgtctt catagaaata gatcgattat cttttaatac
      781 cctactcacc gtcgaagggt taacacctgc cttttgagcg acatctttta ttgttaccat
      841 attaacctcg ttactatatt taccaaagat aaaccttatc atgggtttgt catgtatttc
      901 gtttccatta tacaattatc tttcaaaaaa tcaacttcaa tcaagattcc cttgtcattt
      961 taactcaaat tctctaaact atttttttaa aaaaacgacc tttttttaaa aaaaggcttg
     1021 actttttctg caagcggttg cataatatta agtgtaagaa atatatctta ggaggaaata
     1081 gctatgaaat catggcaaaa agttatcgtc ggcggagcaa gtttgacact tgcaagtacc
     1141 ttgttagtgg gatgtggatc aggttcaaaa gataaaaagg aagctggcgc tgactcaaag
     1201 actattaaac tttgggtacc gactggctct aaaaaatcat atgcagatac cattgcaaaa
     1261 tttgaaaaag attctggtta caccgttaaa gtggttgaat cagaagatcc aaaagcacaa
     1321 gaaaaaatca aaaaagatgc ctcaactgct gccgatgtct tttcacttcc tcatgatcaa
     1381 cttggtcaac ttgtagaatc tggtactatt caagaagttc cagaaaagta caataaagaa
     1441 attgctgcta cttctactga tcaggcgctt atcggtgcac agtacaaagg gaaaacatat
     1501 gctttcccat ttggtattga atcacaagtt cttttctaca ataaatcaaa attagctgct
     1561 gaagacgtta cttcatatga tacaattact acaaaagcaa cttttggtgg tacctttaaa
     1621 caagctaaca cttacgctac tggtccatta ttcatgtcag ttggtaacac tttatttggg
     1681 gaaaacggtg aagatgtaaa aggtactaac tggggcaatg aaaaaggcgc tgctgttctt
     1741 aaatggatcg cagaccaagc ttcaaataaa ggttttgtta gcttagatgc taacaacgtt
     1801 atgtctaaat ttggtgacgg ttcagtagct tcctttgaat caggaccgtg ggactatgaa
     1861 gctgctcaaa aagctatcgg taaagaaaac cttggtgttg ctatttatcc aaaagtaact
     1921 attggaggcg aaacggttca acaaaaagca ttcttgggtg taaaacttta cgcagtcaac
     1981 caagcaccag ctaaaggtga tacaaaacgt atcgcagcta gctacaaatt ggcatcctac
     2041 ttgactaatg ctgaaagcca agaaaaccaa ttcaagactc gtaacattgt cccagccaac
     2101 aaagaagttc aatcttcaga agctgttcaa tcaaacgaac ttgctaaaac cgttatcact
     2161 atgggttctt cttcagacta cactgtcgta atgccaaaac ttagccaaat gggcacattc
     2221 tggactgaaa gtgctgctat tcttagtgat gccttcaacg gtaaaatcaa agaaagcgac
     2281 taccttacta agttgcaaca attcgacaaa gatatcgctg caacaaaata atatgatctc
     2341 atgtcatcac tgctatacta aaacaagcac aaggtaagta cttctaaaaa gttctttcca
     2401 cttaccttat gaagagtgga actaggctcc atcacatttt attcaagctt agtctcactc
     2461 ttacttgttt ttgttttact agtagcttag tgctacttaa atttttaact ctttaaaagt
     2521 ttacgtaaca cttagctatt tcatcctaac aagagattga atctgaacta ccactctagt
     2581 atcctaaaat aaaggagaat ctatatgact cagtcaaact tgacaccaaa tatatctgta
     2641 atagaagcct taaaaagagg cagttgggat attaaacttt cgagtattat catgggattt
     2701 gccaactttg ctaacaaaca gtttatcaaa ggaatcttat ttttaataag tgaacttatc
     2761 tttctagtag cttttgtctc ccaaatcatc cctgctatcc gtggccttgt cactcttggt
     2821 actcaaactc agggaatgac tacaaaaact attgatggga ttaacatcca agttgctgtt
     2881 gatggcgata actctatgct aatgcttatt tttggtttag catcactcat tttctgccta
     2941 gtctttgctt acatttactg gtgtaatctc aaaagtgctc gtaaccttta tcttttcaaa
     3001 caaaaaggtc agaagatacc aagcttcaaa gaagatcttg ccacacttac aaacggtcgc
     3061 tttcatatga ccttaatggc tatcccactg attggtgtgc ttttattcac cattcttcct
     3121 ttgatttaca tgatctgttt ggcctttacc aattttgatc ataatcactt gcctccaaaa
     3181 tctttatttg attgggtggg acttgctaac tttggaaatg ttttaagcgg ccgtatggca
     3241 ggaacctttt ttcctatctt ctcatggact ttgatttggg ctgtttttgc cacagtaact
     3301 aatttcttct ttggtattat tcttgctttg ttaatcaata ccaaaggatt aaagtggaaa
     3361 aaaatgtggc gaaccatctt tgttatcacc attgcagttc cacaattcat ctcattattg
     3421 attatgcgga acttactcaa tgacgaaggc ccactaaatg ctcttctcaa taaaatcgac
     3481 ttgattaatg gctcactgcc atttttatcc gatcctcttt gggcaaaatt ctcaatcatt
     3541 tttgtcaaca tgtggatcgg tattccattt accatgttga ttgcgactgg tattatcatg
     3601 aaccttccaa gtgaacaaat tgaggctgct gaaattgatg gcgctagcaa attccaagtc
     3661 ttcaagtcta tcacgttccc tcaaattctc ttgatcatga caccaaactt gattcaacaa
     3721 tttatcggaa atatcaataa ctttaacgtc atttacctcc ttactggtgg tggtccaaca
     3781 aattcagaat actatcaggc aggaacaaca gacttgctgg tcacttggct ctataaatta
     3841 accgtcacag ctgctgacta caatttagct tctgttatcg gtatcttaat ctttacagtt
     3901 tcagctatct ttagcttact tgcttataca aggacagcat cctacaagga aggagcggct
     3961 aaataatgaa aaacaaacgt cgatttcaac ttggacttgt ttatgctact ttaatcatcc
     4021 tctcaatcat ttggctcttc ccaatcgctt gggttattct tactagcttc cgtagcgaag
     4081 gaactgctta cgtcaattat tttattccta agacttttac attaaatcac tacattaatc
     4141 tttttactaa tgaaaccttt ccttttggaa aatggtttat gaatactctt attgtagcca
     4201 cttttacatg tattatttca accttcataa ctgttgccat agcctactct cttagccgaa
     4261 ttaagtttaa gtttcgcaat ggctttttaa aactagccct tattttaaat atgttccctg
     4321 gtttcatgag catgattgcc atctactata tcttaaaggc cttaggtttg acgcaaaccc
     4381 taacagcact tgtccttgtt tattcctcag gtgcagccct tggtttttac attgcaaaag
     4441 gattttttga taccattcct tattcacttg atgaatcagc tatgattgat ggcgctactc
     4501 gtatggatat tttctttaaa ataacccttc ctttagcaaa accaatcatt gtctatactg
     4561 cccttcttgc ttttatgggg ccttggattg actttatctt tgcacaagtt attttaggtg
     4621 atgctacaag taaatatacc gttgctattg gactattttc aatgcttcag ccagatacca
     4681 ttaacaactg gtttatggcc ttcactgctg gttctgtctt aatcgccgtc ccaatcactc
     4741 tcctcttcat gtttatgcaa aaatactatg ttgaaggcat cacaggtggt tctgttaaat
     4801 aagttacaac ataccaaacc ttctaagggc aagcccttta ggaggtgttt gataaataag
     4861 gccgtagata atcatattag tcctcacatt tatcttcaga tcccgcgttg taaaatgaag
     4921 tgcaacaaaa aagacatgtt cgtctgatat actagaattc cccaattcag tatggaaagc
     4981 agatgaatat gtctaatatt aattctacca gaaaatcctc ctattctcat ctttcagcca
     5041 cagaacgggg tgaaattgct gcttatctta aaatgggaaa gaaacccgtt gagattgctc
     5101 gacttctggg ccgtcaccgt tctacaatct gccgagaaat aaaacgtggt tcagtagatc
     5161 aggtaaagga taagaatgga aaacaaacct tcttcaacgc ctatttcgct gatagtggac
     5221 aacgtgtcta tgaaaccaat cgatttgcct aagatggtta gtattagaaa acgttctaag
     5281 aaagtcatga aaacgaataa gaagacttta ggtaaatcta tcgaagaacg tccagaatat
     5341 attaatgatc gttctgaatt tggacattgg gagattgatt tggttctcgg caagaaaacc
     5401 aaaggtgaag ctgttatgtt gactttggta gagcgccaga cacgctatgc actaggagtt
     5461 aagctagaag acaagcagtc ccaaaccatt aaccgtgctg tcacgcatct catcagtcat
     5521 tatcctattg catccatcac agcggataat ggttctgagt ttagtttact ctcagactta
     5581 aaagctgttg acgtttactt tgcacatccc tattcttcac atgagagagg aacaaacgag
     5641 aacttcaatg gcctcctcag agaatatgtc cctaaaggaa tctcacttaa tccactaacc
     5701 tctgaagaac ttgacaatga tattactgcc atcaatgagc gcctaagacg acttcttcaa
     5761 tagcaatcct caaaattcct gtttgagcta tcccgaacag cttaacctct ggaactctag
     5821 ttatctatga actcgttgca cttgacttga caacttgcga gagcatatca gtaatttttc
     5881 tgctaattga aaacagggta tttactgtca cacaagtctg tcctttcttt ttttctacaa
     5941 ctttatttcg tcagcgtaaa taaaacaaaa gctctataat ctcttgaatg ggtaaatcca
     6001 ctgtagagat tatagagctt tttgaatatc aaaaaagtcc cataagattt ataatgaaaa
     6061 gcaaccaaac tctcattaga aagactctta tggaacatat caatcatacc ttacaacaca
     6121 tcggaattaa agacaaaaat atcactctaa acaatgtttt gaaggatgaa tctcctactg
     6181 agctcgttac tacttatcag aaaggtagat aggaaatcct gatgaagagt gtgtccaagg
     6241 gattatcaat taggtcttag attacaaaat agtaattcag gtatgctcta ctgttttcaa
     6301 cactagttct gcaatttgtt ttcgagcttg cttagcttct cttattggaa aaataacata
     6361 cgcatgaatc attttttctg caataatcag ctgattagca attcgttgtc ttgttaattg
     6421 atcatgtaaa gacataatgt ctgggtaaaa aatctctttt gtcccaacaa aggtcgttat
     6481 ttcaggcaaa tcctcataat caccatacaa aggactgaca taaggaagtt gcatattacc
     6541 ttgaccacct gcccatagtt cacctatatc acgaagacga cgagcatcta ggtaagcttc
     6601 ttctgcttca aaatccttgt aggcttgatt tttaaaacta acatctaacc aaggagatat
     6661 aagaattaat tgctttggct gtggtgctgt tttagtatct cgtatatact tagcaaacgc
     6721 caaagccata cccccaccag aagaatcacc aatgacacta acttgactcg gtccattaga
     6781 caagcaaaat tgctggtaag cttcaactaa tttaggatag acatcttgat aagtagcata
     6841 cggtacttta ggaaagttag gaaaaacaac cttggcatta gtcagctgta taagatgctt
     6901 aaccgtctta aaatgaagtt tttcgggtcg tcgtaaatag acactgccat aaagataata
     6961 aataacaggc tgatgacttt gcttttgatc attaataata aaaacttgca tcccgttcac
     7021 atcgtattct tgagttgaaa aacctactat ctcttcagga ttatctagat gataagggat
     7081 attacgatta cgctgcgtct caaccccctt ataattatca acttttttag ataacttata
     7141 ctttctataa gcaaacattt tctcaaccaa ccaacttgaa aaggagcgct tttgaccata
     7201 ttcaactatc aaaataccta gtatcactcc aaaaaaaatc accacagcaa taatgaccac
     7261 ttattatcct cgttttctaa tattatttta ctattgcaag tctttcctat ttgcccaaat
     7321 tttactataa aaataagggt tcattttata gttgggtact ggctgcttcg tttctagaaa
     7381 tggttgcact ttcttatcaa aagctaacca gccattccaa ccgagatgga tggtatcttg
     7441 cataaagtag gactcaccac catcttttga gaagtcagca atgcgatgaa atccttgtga
     7501 ctttaactgg aattttattt tacgaactgc cgcttgatac ttatcttgat ttaagccggt
     7561 ataatccgcc caagctttat taacaggagt tataacaaag agtacatctg tttttcgttt
     7621 agcaaattct gataacaaaa gctgaaaatc attgtattct ggtgacgcca ggtaactata
     7681 attaacttgg aaattcttat aaagattgta tttaggtgct attcgtttac gataaaatgt
     7741 atttttaatc ccaaaacggt tgttggttgt tgctaattgg cctctcttag ttgctaatgc
     7801 attcaattgt ttatacgaaa atactttagg taatccctta acgcgaggca aaattctttt
     7861 ttcatagtta gtagatttgc ctaaaaaact aaaaagggac tcttctctca atgctacttg
     7921 atgttgacat ttcaaaatag ctctgtctaa ccgactaaga gacttaccct tacttacttt
     7981 tttcaataag tttgatttag acacaccagg gttaagctca agcaaacgct ttgctgcaaa
     8041 ctgtgattct ttatcagttc tagcttttag taaaaattca atcacttgag tgttagacaa
     8101 gtacatctga accgcactag gattaatccc ttgagcagta aaccattgag gagatactac
     8161 aaagatggct tttttctttt gcatttcatt ggtaatttgt tgcataccat aataatgcga
     8221 caaagatgct gatcctctct taccaattaa aaatggtcta tagctccgct tgtagcgctc
     8281 tgcaagcacc gaagggtgca tactatccat tcgactccat tcgctagaac caaaaaaagg
     8341 cacaaaacga caagtctcat ctgataaagc ttgtctttta atcaaaccat ttttaaaaga
     8401 actatctgtg atcgcaacgg catttgcttt ttcctgagct atggaatgat caagttgtgt
     8461 gggaaaacta aaaatagtaa tcactactaa aacaaaggca ataagaagag gacctagaat
     8521 taaccagagt ctcttaagca tgttgcaatt cctctacacc ttgaacaatc ttagttactg
     8581 tgttccaatc atcacgacca aattctgaaa taggtacttt aatattgaac gttgactcta
     8641 actcaacaat caattcgact gtcccaagac tatccaacac acctgcatca aacaagtctt
     8701 cgtccatcat ctctgaaaca tcttccataa acaaacggtc aaataattca attacagttt
     8761 cttcaataga catttttata cttcctttta ttaatgtagt ggtctgttaa accaaaggtc
     8821 atttaaaaaa ccagagaata acagaagtga aaacatgaca acgtggaaag ttaccacgat
     8881 acataagaca tggaatgccc tgctttgaaa gaggggtgat aagccttttt tcttacggtg
     8941 acggttgatt tcttttttct tacgaatcca agcatcatta ataataagtc ctataccatg
     9001 gaaaagccca taagcgatat aataccaggt taagccatgc caaaatccca tgactagcat
     9061 attaaccaga taggcaacac ctgaagtgac atttcggttt ttaaaggttt tatgcttaat
     9121 caatagatga accaatcgca taaacacata atctctaaac cagaaggaca aactcatgtg
     9181 ccatctattc caaaaatctt tcaaacttgc agataagaaa ggcaggttaa agttttctgg
     9241 tgttcgaata cccaataggt aggaaatacc aatcgcaaac atcgaataac cagcaaagtc
     9301 aaaaaagaga ttcaagccat acacgtacat aaccagaata gtttccttat taaagtagcc
     9361 accaaccatc aaagccttat tttcaaggag aggaagtaag atgccaccca aacaatagga
     9421 aatgatatgc ttgtatagga aacctaacat gagatacatg actgccttat tgagcattgc
     9481 aaaataatca tcacgttcag gaagtttatg ataatcttcc tgaaaacgtc tgaagcgatc
     9541 aatggggcca cttgagaaag tggggaagaa aatcatgaag cgaataaaat ccggtaaacg
     9601 aacctcttgg agagtcccgt cacgcatctc aataatcatt ccaatacttt taaaggtcaa
     9661 atatgaaatt cctagaaaac taaagagtgt ttgagaagaa ggacctgtta ggggagcaac
     9721 tttgacccag ataaggggga ataaggccat cgcaatagct aagtaaaaca ctcctgcact
     9781 attagctttc tcacggtatt tctggtaagc aaaaacactg atgatttgcc acaaaagata
     9841 aaagagaaaa gcaactaatt gttgataatg atctcctcca aacataaaga gaataaatac
     9901 caaactcacc aaggtttcat aagtgcttaa gcgcttttgt ttaaaaatcc caatgatgac
     9961 tggtaagaaa gccaaaataa gatagacaaa gtaaatgggg ttaccgtaag gttccatgta
    10021 gggaatgtgg ctaaaaaaca tcatcatcgg ttattgacct cattaatcaa agttttaata
    10081 tctatcttac catttggagt taatggtaga ctatctcgat aaagaaactt agaaggcatc
    10141 atataactca tcatatgatc cttaacagag gctttgatag ctttggttaa ctccaattcc
    10201 ctatcaaaac gctctttaac cccatctttg acaacgatat aggccaagag attttgaacc
    10261 ttatgttctt tattgtacct tggaacagcc acagccgatg ctaccattgg tgattgattc
    10321 aactgttggg aaacatcttc tagttcaata cgataaccag catatttaat ctgaaaatcc
    10381 aaacgcccac catacagcaa aatattatct tctgtcaatg agccaatgtc ccctgtatga
    10441 taagcaggct gtcctttaaa agtaaagaaa gcttctgcgg ttttctcagg attgttgagg
    10501 taaccttttg acacagcagg ccctgtaaca ataatttcac cctgctcccc tgaagataac
    10561 tccttaccat cttcatcaat aatataggtc ggagaatctg gtttggggta gccaatcggc
    10621 aagcgggtat aattatccac catttctcta gtaatttcaa tagcggataa ggctacagtc
    10681 gcttctgttg gtccataggc attgataatc ttggcactcg ggaaacgttc aaagagtttt
    10741 cgagccgtag aaacggttaa ttcttcgcca tcaaaataaa aatgcgttaa agctggcatc
    10801 ttagcctggc aaaaatcatc gctaagcatg gccatatcag caaatgaagg agtcgatgtc
    10861 caaatgccaa ctggaagttg cgcaatcgtt gtaaataact gtttaaagtc tgccaccaat
    10921 tcttttggaa gtgcaaataa ggttccacct aaagccaagg taggtgccca gtacatgaca
    10981 gaaaggtcaa aggaataggg tggctgcgcc agcatttgtg gctgtttagg tacatcaaat
    11041 gctgcatctt cgatcatcca gttggtaaag ctcaaaagat tgtcatgtga aatttgcacc
    11101 ccttttggtt ggcccgttgt tccagaggtg aaaataatgt aataattatc atctccttta
    11161 acagaatgtg ttcgctcata gggcatttct gctaattttg ctgactcaat ctctgatagt
    11221 gagacaaggg aaatcccttc aatagtaaga gggaattcct caatagcaat gattaaactg
    11281 ggctttgcaa tctcaataat cgctaaaata cgttctggtg ctgaatgcac atctactggg
    11341 atataggcat gaccagattt ggttaaagct acaaaagtag ctaacatatc ataagtttgc
    11401 gccccaaaaa ccaaaactgg agattttgcc agtaaagcta agctatctat aaatgcagca
    11461 atgctatcag aatctctttt gagttgtccg taagtgcggc gttcccctaa acaatcataa
    11521 actggaaaat cagcctgtgt ctgagcaaac tgctcaatac tgtcaatcat atccttaatc
    11581 atcagtcaat ctcctaaaat tcattataaa taaagttact ttgaccatgt cctagataac
    11641 caaagaaata gaccagcaac attaaaatca gataaaataa aattgtcttg ccaataaaaa
    11701 cagcaattcc tctcattctt gatttgtttt ttatcatact ttaccttctt gtattagtga
    11761 aa
//
LOCUS       DAUGHY010000044        11624 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.044, whole genome shotgun sequence.
ACCESSION   DAUGHY010000044 DAUGHY010000000
VERSION     DAUGHY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11624
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.044"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..388)
                     /locus_tag="VH752_001336"
     CDS             complement(<1..388)
                     /locus_tag="VH752_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ8289682.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCYKLIPNQFGLIVSGII"
     gene            complement(446..991)
                     /gene="coaC"
                     /locus_tag="VH752_001337"
     CDS             complement(446..991)
                     /gene="coaC"
                     /locus_tag="VH752_001337"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEQ8289683.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     GPGALADIDVILATIDTIWKL"
     gene            complement(984..1676)
                     /gene="coaB"
                     /locus_tag="VH752_001338"
     CDS             complement(984..1676)
                     /gene="coaB"
                     /locus_tag="VH752_001338"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEQ8289684.1"
                     /translation="MTMKLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHD
                     VTLVTTKTATKPLPNEHLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMT
                     DLEQVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNPNIKLVGFK
                     LLVNVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAI
                     ADLLYERMTKHD"
     gene            1857..2708
                     /locus_tag="VH752_001339"
     CDS             1857..2708
                     /locus_tag="VH752_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEQ8289685.1"
                     /translation="MKGRIVITIRDLASLPIFVYGDGNKKVPGALSPFVWTEVFLKTI
                     NQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKKEIGNNGYVPVTRNIGGLAVVADD
                     GILNFSLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLS
                     IAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKAFYEAGTQGEVTKVNYPQID
                     PECMATLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLE
                     VVRKEGK"
     gene            2705..3904
                     /locus_tag="VH752_001340"
     CDS             2705..3904
                     /locus_tag="VH752_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEQ8289686.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     ISYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAV
                     LQLTHAGRFASYALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVIKQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNVAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            3901..4899
                     /locus_tag="VH752_001341"
     CDS             3901..4899
                     /locus_tag="VH752_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEQ8289687.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMPLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEKAEALAKPLDIWMLGQQDFNEFKTYPEVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            4941..5273
                     /locus_tag="VH752_001342"
     CDS             4941..5273
                     /locus_tag="VH752_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEQ8289688.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVERNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            5266..6078
                     /locus_tag="VH752_001343"
     CDS             5266..6078
                     /locus_tag="VH752_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEQ8289689.1"
                     /translation="MPSSFDLLGEMIGLLQTEQLTSSWACPLPNALTKRQDLWRALIN
                     QRPALPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIV
                     NAANSELLGCFIPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHL
                     PASYIIHTVGPRITKGHHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGF
                     PKKEASQIAIKTVLKWQAEHPESKTLTIIFNTFTSEDKTLYDTYLQKENNCE"
     gene            6071..6952
                     /locus_tag="VH752_001344"
     CDS             6071..6952
                     /locus_tag="VH752_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEQ8289690.1"
                     /translation="MSNWTTYPQKNLTQAEQLSQLIKEADALVVGIGAGMSAADGFTY
                     IGPRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILETKDYHIITTNADNAFWMAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKL"
     gene            6999..8018
                     /locus_tag="VH752_001345"
     CDS             6999..8018
                     /locus_tag="VH752_001345"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ8289691.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KRTYQPAIEALHHLGATGVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREYLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAIDLAPYLGAITAEELG
                     DLIFS"
     gene            8146..9816
                     /locus_tag="VH752_001346"
     CDS             8146..9816
                     /locus_tag="VH752_001346"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ8289692.1"
                     /translation="MKSDIEIAQSVALQPITDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLILVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKSDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGLALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYGGKAVQFGPKAKTQLKQFAEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            9901..11478
                     /gene="cls"
                     /locus_tag="VH752_001347"
     CDS             9901..11478
                     /gene="cls"
                     /locus_tag="VH752_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEQ8289693.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVWITILESVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYT
                     KLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
                     NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVE
                     VRVLFDGMNELSTLSSDYAKRLEQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEV
                     SFTGGINLADEYINEVERFGHWKDAGLMLEGEATDSFLILFLQMWSITEKELIIDPYL
                     SDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHA
                     LRFASERGVDIRIIMPGVPDKGVPYALAKTYYKALMTSGVKIYEYQPGFVHSKVFISD
                     NTKAVVGTINLDYRSLYHHFECATYLYRVSVIADIVNDFNEAQKQSLLMTSDHLTQRP
                     WYQKLIGLLVRTIAPLL"
BASE COUNT         3588 a         2025 c         2445 g         3566 t
ORIGIN      
        1 caatgatacc ggatactatt aaaccaaact gattaggaat caatttatag caataataag
       61 gggtaatacc aatcaaaata cgtgggataa tagcaattat taaagaagcg aaggttccat
      121 gatcgacaaa aggtgaaaag agataatttg taggcagtag aatgatggta tttgtaatga
      181 cacttataat ccccattaat cctcctaaaa tagcacctat ccttgggcca tataagacag
      241 aagcaataat aactgggata tggacaagcg ttggtttgat tggaataggc caaatattaa
      301 agacaagact gctcacaaaa tgaatgacta acatgatggc aaaaaagata gcaatacgag
      361 aaatatctgc tgatttacgg tgattcattt tactctccaa aaaatataat acttaattct
      421 aaaaaggtga tcagaaaaac gactgttaaa gtttccaaat cgtatcaata gtagctaaga
      481 taacatcaat atcagccaaa gcaccaggtc ctttatctcc acaggctaat aagctagatt
      541 ttggaggaat ttctgtaaac cctattgtcg ataagcgttt aatattttct tgggtaatag
      601 ggttttgata cattttggta ttcatggctg gggctattaa ttttggtgtg gtagctggta
      661 gggcaagagc aacacttgtt acaatattat cagcgaatcc atatgctaga tgagcaatag
      721 tattagcaga ggcaggtgca acaataaata aatctgtgcg ttttgctaat tcgatatggt
      781 tgataacttt tgggtcatgc tcatccataa catctaaatg aatagcattt tttgataaca
      841 cttgcagggt taatggtgta ataaattgtg tagcagcctg agtcattatg atgtgaacat
      901 catagccaat tttagttaat tgactcgtaa gatctgctgc tttataggca gatatactac
      961 cagaaacagc taaggtaata tgtttagtca tgtttggtca tcctttcata taataagtca
     1021 gcaattgcct ctttggtatc tgcagatgca acttcgttat tggatatcaa aagagccttg
     1081 tgcatacctg tttggatatc aactaaatca ttggcgagaa tataatcagc atggttttta
     1141 gctagacttg ctctggctac tttaattaac tcttcttgag gcacattaac tagaagttta
     1201 aagccgacta gtttaatatt aggattccac tgtttaacat aagagataac tttgggtgtt
     1261 tttttcaaaa aaagaacctg ataatcagat gcagatgaga ttttcggctc actgttatgt
     1321 tcacatagaa aacaattaag gttatcagct tgagagactt gttccaaatc agtcatgtag
     1381 actggagtat agtcagaaac agccatacta tgaattagaa tgtcatggtg aggaacctga
     1441 tctttgagtg cagccattaa gtcgttaact gtttccactt caataatcct taggtgctca
     1501 tttggcaggg gtttagtggc tgttttagta gtgactaggg taacgtcatg atgatattgt
     1561 aaaaatcgct ctgtgattaa ttttccaagt tgcccagttg agtgattagt gatacctcgt
     1621 actgcatcta taggttcagt tgttccacct gatgttatta ttaatttcat agtcatattt
     1681 tatcaaaaaa ggagctgata gtaaacggaa gataatgacg ccagacattg tcttgcgttt
     1741 gtattaagat ggaatagtaa tagatttcta gtaattaaaa cgaagttctg gaaagcaaga
     1801 cactatagta gctaaaatct aaattttaag gtaaaatagg gcaagcggtt tcaagtgtga
     1861 aaggaagaat tgtgataact attagagatt tagcgtcact gccgattttt gtttacggtg
     1921 atggaaataa aaaagttcca ggagctttga gtccttttgt gtggacagag gtgtttttaa
     1981 aaactatcaa tcaagaacct aatcaactga tcttacatat ttggccgatg acaagaacag
     2041 ttatattagg aatgttagat agacagcttc cctactttga gctggctaaa aaagagattg
     2101 gcaataatgg ttatgttcct gttactcgta atattggcgg tttggctgtt gttgctgatg
     2161 atgggatttt gaatttttcg cttgttattc cagaccattt ttcggaatct atcagtatta
     2221 gtaatgctta tttaataatg gttgatgtca ttagagagag tttttcggat tattatcagc
     2281 gtattgaata tcacgaaatt aaaaattctt attgccctgg taattttgat cttagtattg
     2341 caggccgcaa gtttgcaggt atcgcccagc gtcgtatcaa aaagggaatt gttgtgtcta
     2401 tttacttgag cgtttgtggg gatcaagcag cgcgtggtca actcatcaag gctttttatg
     2461 aggctggaac acaaggagaa gttaccaagg ttaattaccc tcaaattgat ccagaatgca
     2521 tggcaactct ttcagaactc cttgagacgc cttttacagt ggcagaagtc cttgaacgtc
     2581 ttcgattgac cttgcgacag cttggctttt ccctcacgga aaaaagtcca gatcaagcct
     2641 tactgactaa ttttgatgct gtctatgaaa ggatgcagct agaagtcgtt agaaaggaag
     2701 gcaaatgaaa gaaagattta gtcctttatt tgagcctttg actttgccaa atgggagtca
     2761 attggataat cgctttgttt tgtctcctat ggtgaccaac tcatccacaa aagacggtta
     2821 tgtcacccaa gatgatatat cctatgcttt gagacgagca gcttctgccc ctctacaaat
     2881 tacaggagcg gcctatgtgg atccttatgg tcaattgttt gaatatggtt ttagtgttac
     2941 aaaagatgcc gatatttctg gcttgaaaga gctagctcaa gcgatgaagg caaagggagc
     3001 taaagctgtc ttacaactga cccacgctgg ccggtttgct agctatgcct tgactaaata
     3061 tggctttgtc tatgggccta gttacatgca gttgagatct ccccaacctc atgaagtcaa
     3121 acctttaaca ggtcagcaaa tagaggaatt gattgcagct tacgctcagg ccactcggcg
     3181 ggcaattcaa gcaggctttg atggcgtgga agtatcttcg gcgcagcgct tattgattca
     3241 aacgttcttt tcgacttttt cgaacaagag gacagatagt tatggttgtc aaacactttt
     3301 taatcgctct aagttaactc tagctgtttt gcaagctgtt caacaggtga ttaaacaaga
     3361 agctcctgat gggtttattt ttggatttcg tgctacacca gaagaaacta ggggaaatga
     3421 tattggttat agtattgacg agtttttgca gttgatggat tgggtactta atgttgcaaa
     3481 acttgattat ttagcgatag caagttgggg ccgtcatgta tttcgaaata ctgttcgttc
     3541 tcctggccct tattatggaa ggcgagttaa tcaggtagtt agagattatt taagaaataa
     3601 gctacctgtt atggctacag ggggaatgaa cacacctgac aaagctattg aagcactagc
     3661 tcatgctgat tttattggag tatctacacc ttttgtcgtg gatccagaat ttgctcataa
     3721 aatcaaagaa gggtgtgaag agagtattca tttaaggatt cgacctgctg atttgaaaag
     3781 tttagcgatt cctcaggctt ctttcaaaga tattgtacct ttgatggact acggggaatc
     3841 attgcctaaa gaatcaagaa ctttatttcg tagtcttact cacaattata aggagattaa
     3901 atgaaagtca gtattcttga ttatggggtg attgataaag aaaaaacacc ccaagaagct
     3961 ttattagaaa cgcgctgctt agcacaagta gccgacaaac ttggttttca tcgtttttgg
     4021 gtagcagagc atcataatat ttatgccttt gcaataagca gtccagagct gttgatgatg
     4081 catttagcag atcataccaa acagattcgg ataggttcag gaggtattat gcctctacat
     4141 tatagtagct ttaagattgc agaatggata atgactttag aggccttgca ccctaatcgg
     4201 attgatttag gtattggcaa ctctttagga actacgttag ttcaaagagc tctatcaagt
     4261 atccattgca aagacagcta tagtcaggtt gtcacagaat tataccagta tcttaatcct
     4321 gatcatttgt caccactacc gatttttgtt aaccctaggg gtaatactta ccctcagatt
     4381 tggacgctaa gcaatagttt ggagacagca gaactggctg gtcaattagg tttaggctat
     4441 acctttggta ttttccccta tataccaaaa gatcctataa cagaagctaa aagagtatcg
     4501 gcacattata gaaaggcttt taggccttca aagctattaa aaatccccaa gttaatatta
     4561 gcagttttta ttgtcctttc tgataccgac gagaaagcag aagctttggc taaaccgttg
     4621 gacatttgga tgcttggtca gcaagatttt aatgaattta agacctaccc tgaagttgag
     4681 gaggctagaa actatcactt aacagagaaa caaagagaag ccattgcagc aaatcgtagt
     4741 cgcatggtta ttggatcacc tcacacagtc aagaaacaat tggataggct gatagaagct
     4801 tgtcaagctg acgaattatt agccatcccc ttagttcctg aatttgccaa tagacaacga
     4861 accttagagc tgctagcaga cttatacgtg accatttaac cttaccaaaa acaattccct
     4921 aaactgaaag gaaagaaaca atgaaaaaaa ttgcaaatta cttattaatt gagaaaacag
     4981 atgaccgcta taccattagc atgacaccag aattacaaga cgatattggt actattggtt
     5041 acgctgaatt tacggataac gaccaccttg ccgttgatga cattatctta aatctagagg
     5101 cttctaagac cgtcatgtct gtcttgtcac cattggctgg ggcagttgtt gagcgcaatg
     5161 aggcagccac actaacacca acccttttga attcagaaaa agctgaggaa aattggattg
     5221 ttgtgctaac agatgttgat caagcagctt ttgatgcctt agaagatgcc tagttcattt
     5281 gatttattag gggaaatgat tggtctgttg caaacagagc agcttactag ttcttgggcc
     5341 tgtcccttgc ctaatgcttt aacaaagcga caagacttgt ggcgggcgct cattaaccag
     5401 cgccctgctt tgcctttatc aaaagattac ttgaatctgg aagatgctta tctagatgat
     5461 tggcgtgctt cttttgttcc tgtatcagtc aaggattgcc aaaaaactaa ttataccagt
     5521 ctttttcttt accatgggga tattaggtac cttgctgtgg atgctattgt caatgctgct
     5581 aatagtgagt tattgggatg ttttattcct aaccatggct gtattgataa tgcgattcat
     5641 acgtttgccg gaagtcgttt acgcttagct tgtcaggcaa tcatgactga acaaggccgt
     5701 aaggaagcca ttggccaagc aaagcttacc agtgcttatc atctaccagc ctcttatatt
     5761 attcataccg tagggccaag aattaccaaa ggacaccacg tttctccgat tcgagctgat
     5821 ttgttagcac gttgttatcg ttctagtctt gatttggcgg ttaaagcagg attgactagc
     5881 ctagcttttt gctctatttc aacaggagaa tttggctttc ctaaaaaaga agcctctcag
     5941 attgctatta agacagtttt aaaatggcaa gcagaacatc cagaatctaa gaccttaact
     6001 atcattttta atacctttac atcagaagac aagaccttat acgacactta tttgcaaaag
     6061 gagaataact gtgagtaatt ggacaaccta tcctcagaaa aacctcaccc aggcagaaca
     6121 actgtcccaa ttaatcaaag aagcagatgc acttgtagtt ggtattggag ctggcatgtc
     6181 tgcagctgat ggttttactt atatcgggcc gcgttttgag acggcattcc cagattttat
     6241 tgctaagtat cagtttttgg atatgctgca agctagcctt tttgattttg aagattggca
     6301 agaatattgg gcttttcaaa gtcgctttgt cgctttgaat tatcttgatc agcccgtagg
     6361 ccagtcttat cttgatttaa aagagatttt ggaaaccaaa gactatcata ttattactac
     6421 taatgctgat aatgcttttt ggatggctgg ttatgatcct cataacattt ttcacattca
     6481 aggagaatat ggtttgtggc aatgtagcca gcactgccat cagcaaactt ataaggacga
     6541 tacggtcatc cgtcaaatga tcgcagagca aaaaaacatg aaagtcccag gtcaactgat
     6601 tcctcattgc cctgaatgtg aggcgccttt tgaaatcaat aagcgtaatg aagaaaaagg
     6661 aatggttgaa gatgctgact ttcatgccca aaaagcacgt tatgaggctt ttttatcaga
     6721 acataaagag ggtaaagtgc tttatctaga aattggtgtg ggacatacga cgcctcagtt
     6781 tatcaaacac cccttttgga aacgtgtctc agaaaaccct aatgccttat tcgttacctt
     6841 gaatcacaaa cattaccgca tccctctgtc aataaggcga caaagcctag aactgacaga
     6901 acacattgca caattgatta gcgccacaaa aaccatttac caaaaattgt aaccaaagcc
     6961 aatagacggt taattaagag atagagaagg agactaacat gtaccttatt gaaccgattc
     7021 gtaacggtaa acgtatcacc gatggagctg ttgctctagc catgcaagtt tatgttcaag
     7081 aaaatctatt tttagatgat gatattttat ttccctacta ttgtgatccc aaagttgaaa
     7141 ttggaaaatt ccagaatgca gttgtagaaa ctaatcaaga gtatttaaaa gagcatcata
     7201 tccctgttgt tcgcagagat actggcggtg gtgccgttta tgtagattct ggtgctgtta
     7261 atatttgcta cttgattaat gataatggta tttttggtga ttttaaaaga acctatcagc
     7321 ccgctattga agcccttcat catttgggag caacaggggt tgaaatgtct ggacggaatg
     7381 acttggttat tgatgggaaa aaggtatctg gcgctgcgat gactattgct aatggccgtg
     7441 tttatggggg atattccctt ttgctagatg tggactttga agccatggaa aaagcattaa
     7501 aacctaatcg caaaaaaatt gaatcaaaag gcatcaggtc agttcgtagt cgcgttggaa
     7561 acatcagaga atatttagca ccacaatacc aaggtattac gattgaagag ttcaaagatt
     7621 taatggtttg ccaattgtta cagattgaga cgattagcca agctaagcgt tatgatttaa
     7681 cggaaaaaga ctggcaacaa attgatgctt taacagagcg taaatatcac aattgggaat
     7741 ggaattatgg caacgcgcct cagtaccgtt accatagaga tggccgtttt accggtggaa
     7801 cagtagatat tcatttagat attaaaaaag gctatattgc agcttgtcgc atttacggag
     7861 atttttttgg caaggcagac attgcagaac ttgaaggtca cttgatagga acacgaatgg
     7921 aaaaagagga tgtcttagct accctcaatg caattgattt agccccttat ttaggtgcga
     7981 ttacagcaga agaattagga gatttgatat tttcttgata aaaaagatac ctcagaccgg
     8041 taaagtgcga ttaaaaacga acaataattg ttttgattca aataaatgta cgtatgatat
     8101 gaattgtgat ataatgacgt cattacgtaa aattgaggag tcatcatgaa atcagatatc
     8161 gaaattgccc aaagtgtcgc cctacaacca atcactgaca ttgttaaaaa agttggaata
     8221 gacggcgatg acattgagct ttatggcaaa tataaggcta aattgtcttt tgagaaaatg
     8281 aaagctgtcg aagctaatga gcctggaaaa ttaatcttgg taactgctat taaccctact
     8341 ccagcaggag aaggaaaatc aaccatgtct attggactgg cagatgcctt aaatcaaatg
     8401 ggaaaaaaaa ccatgcttgc cttacgtgaa ccctcccttg gtcctgttat ggggattaaa
     8461 ggcggggcag caggtggtgg ttatgcccaa gtattgccta tggaagacat taatcttcac
     8521 tttactggtg acatgcatgc cattacaacc gctaataatg cattatctgc tttgattgac
     8581 aatcatttgc agcaaggaaa cgatttagga attgatcctc gccgcattat ttggaagcgc
     8641 gtgttggacc ttaatgatcg tgctcttcgt caggtaattg ttggcttagg cagtccagtg
     8701 aatggtgtgc cacgcgaaga tgggtttgat atcacagtag cttctgaaat catggcaatt
     8761 ttatgtttgg caactgactt gaaagatcta aagaaacgtc ttgctgatat cgtcgtggct
     8821 tatacctatg atcgtaagcc tgtctatgtg cgtgacttga aggttgaagg agcattgaca
     8881 ttaattctaa aagatgctat caagccaaac ttggtgcaga ctatttatgg aacaccagct
     8941 cttattcatg gggggccatt tgctaatatt gctcatggtt gtaattctgt ccttgctacc
     9001 tcaacagctt tgcgattagc agattacact gttaccgaag caggattcgg ggccgaccta
     9061 ggagctgaaa aattcctaaa tatcaaagta cctaatcttc ctaaagcacc agatgctatc
     9121 gtcattgttg caaccttgcg cgcccttaaa atgcatggag gtgtcgccaa atcagattta
     9181 gccgctgaaa attgtgaggc tgttcgctta ggatttgcca acttaaaacg tcacgtggaa
     9241 aacatgcgcc aattcaaagt tccagttgtg gtggctatca atgaatttgt agcagatacc
     9301 gaggctgaaa ttgctacctt aaaagcgctt tgtgaagaga ttaaggttcc agtagaatta
     9361 gccagtgttt gggcaaatgg agctgaggga ggcttagcat tagctaaaac agtggttcgt
     9421 gtgattgatc aagaagctgc tgactacaaa cgtctctatt cagatgagga tactttggaa
     9481 gaaaaagtga tcaatatcgt tacccagatt tatggtggca aagctgttca atttggcccc
     9541 aaggccaaaa cacaattgaa gcagttcgca gaattcggtt gggataaatt gcctgtttgt
     9601 atggctaaga cccaatacag tttttcagat aacccatcct tgcttggagc gccaaccgat
     9661 tttgatatta ccattcgtga atttgttcct aaaacagggg caggttttat tgttggttta
     9721 acaggtgatg tgatgaccat gcctggtttg cctaaagttc cagctgccat ggcaatggac
     9781 gtagcagaaa atggcacagc cctcggtctc ttttaggaaa aattgctcga ataattcgag
     9841 caagcgctcc tttcttcccg ctttactgtg atataataag gtaaatgaga ttttggaggg
     9901 atgattatca aaaagaaagc aaaagtaaaa tatttgctcc ataaagggaa acatggattt
     9961 ttaagaggaa tttttagtag aacaacaatc attgtattat taattatttt gcaattagtt
    10021 ttcttatttc aatcttatgc ctggatggag cagtaccgtg tttggataac gattcttgaa
    10081 agtgtctttg ctattactat tgttttatat ttagttaata gtgatatgga tgctatttca
    10141 agaatgacgt ggttaatttt gattatgatt gctccattac tagggtcact gtttttaatt
    10201 tatacaaaat tggattgggg ttacagaggg ctaaaacaaa gaataaatca tcttgtagat
    10261 ctgtctgcac cttaccttag cgatgatgac gctatattag aggtattaaa ggatagcaca
    10321 tcaaccacat atcatttagt acagtactta gaaagaagtc gtggcaactt tccgatttat
    10381 aataatacaa gggtaactta cttcccaaca ggtgaaactt tttttgatag tttaaaagaa
    10441 cagttattct tagctaaaaa gtacattttt cttgaatttt ttattattgc agaaggccaa
    10501 atgtggggag aaatccttag tattctagaa aaaaaagtca gtgaaggtgt agaagttagg
    10561 gtgttgtttg atggcatgaa cgaactatca acgctatcat cagattacgc caagagatta
    10621 gaacaaatag ggattaaagc taaatcattt ttaccgattt caccctttat ctctacctat
    10681 tacaattatc gagatcaccg aaaaattgtc gttattgatg gggaggtatc attcactgga
    10741 ggtattaatc tagcagatga gtacattaat gaagtagagc gttttggcca ctggaaagat
    10801 gctggtttaa tgcttgaggg tgaagcaaca gacagctttt taattttgtt tttacaaatg
    10861 tggtctatca cagaaaaaga actgattatt gatccttatc tttcagatca ttctttaaaa
    10921 cttccttcag atggctatgt tattccctac ggtgattccc cgcttgatac tgataaaata
    10981 ggtaaaaatg tttatataga cattttaaat catgctaaag agtacgttta tatcatgaca
    11041 ccttacctta ttttagatag cgagatggaa cacgctttac gatttgcatc agaacgtgga
    11101 gtagatatcc gaattattat gccaggagtg ccagataagg gggtaccata tgccttagct
    11161 aaaacctatt ataaagcttt gatgacttct ggagtaaaaa tttacgagta tcaaccaggg
    11221 ttcgttcatt caaaggtatt tatctctgat aatacaaaag cagttgtcgg aacaattaac
    11281 ttagactatc gtagccttta tcatcatttt gaatgcgcga cttatttata tcgtgtgtca
    11341 gttattgctg atattgtgaa tgactttaat gaagctcaaa agcaatcact attgatgaca
    11401 tcagatcatt tgacgcaacg tccttggtat caaaaattga taggattatt agtacgaaca
    11461 attgcccctc tcttgtaatt ttcgtaaaat atgttataat atctgtatgc gatgagtcga
    11521 ttgtgactgc aaagtcactt ttgcgcaggg gaggtcatta aggcgggaag ccgactttga
    11581 taatctgtgt gaaccatttt atcacaatct tatttattaa gaat
//
LOCUS       DAUGHY010000045        11494 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.045, whole genome shotgun sequence.
ACCESSION   DAUGHY010000045 DAUGHY010000000
VERSION     DAUGHY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11494
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.045"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            135..3245
                     /locus_tag="VH752_001348"
     CDS             135..3245
                     /locus_tag="VH752_001348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEQ8289694.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEVEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYLCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELADCQQLTAFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQDLTEAGLKEKGLWKEPYQSRLLHELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDTLTNHIPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDIAAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIASILEPTYGIMLYQEQVMQIAQVYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSDYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIIEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKILDNLDGLLVFVNELGSLFSDSSFSWVDTKDYSATEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            3325..4338
                     /gene="pfkA"
                     /locus_tag="VH752_001349"
     CDS             3325..4338
                     /gene="pfkA"
                     /locus_tag="VH752_001349"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEQ8289695.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            4401..5903
                     /gene="pyk"
                     /locus_tag="VH752_001350"
     CDS             4401..5903
                     /gene="pyk"
                     /locus_tag="VH752_001350"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEQ8289696.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICRETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            6121..6678
                     /gene="lepB"
                     /locus_tag="VH752_001351"
     CDS             6121..6678
                     /gene="lepB"
                     /locus_tag="VH752_001351"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ8289697.1"
                     /translation="MVKRDFIRNILLLLIVIIGAILLRIFVFSTFKVSPETANTYLKS
                     GDLVTIKKNIQPKYKDFVVYRVGKKDYVSRVIAVEGDSVTYMDDIFYLNNMVESQAYL
                     EKMKAHYLNHAPFGTLYTDDFTVATITADKYQKVPKGKYLLLNDNRKNTNDSRRFGLI
                     NASQIKGLVTFRVLPLSDFGFVEVE"
     gene            6854..8668
                     /gene="glmS"
                     /locus_tag="VH752_001352"
     CDS             6854..8668
                     /gene="glmS"
                     /locus_tag="VH752_001352"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEQ8289698.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANAHQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKALSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVIVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LVAEKVQALLATTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVSSHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHSF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            8850..9185
                     /locus_tag="VH752_001353"
     CDS             8850..9185
                     /locus_tag="VH752_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEQ8289699.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVV
                     LDSNGVRLSDGDTITVVKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            9292..9933
                     /locus_tag="VH752_001354"
     CDS             9292..9933
                     /locus_tag="VH752_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ8289700.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGTILAFLMKIPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFIAGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            9943..10572
                     /locus_tag="VH752_001355"
     CDS             9943..10572
                     /locus_tag="VH752_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289701.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQKADAKEKALDLLARLGLKEHAQVYPYSLSGGQKQRVALARAMMIAPQIIGYDEP
                     TSALDPELRQTVEALIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            10588..11424
                     /locus_tag="VH752_001356"
     CDS             10588..11424
                     /locus_tag="VH752_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ8289702.1"
                     /translation="MIIKKRTVAILAIASSFFLVACQATKSLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGKCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQDKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
                     SLLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLEN
                     YRMIPTTFENEAFSVGLRKEDKTLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANI
                     KS"
BASE COUNT         3581 a         1909 c         2450 g         3554 t
ORIGIN      
        1 gaaaaaaaag gggaatgtca agttttatgc catttttcac tctttgagaa gtagacgtag
       61 attagagaga aaattgaagc aagaaaacct ttttcatagt ataataggga acaagataac
      121 caaaggagga acttatgttt gctcaacttg atactaaaac tgtatactca tttatggata
      181 gtttaattga cttaaatcat tattttgaac gagcaaagca atttggttac cacaccatag
      241 gaatcatgga taaggataat ctttatggtg cttaccattt tattaaaggt tgtcaaaaaa
      301 atggactgca gccagtttta ggtttggaag tagagattct ctatcaagag cggcaggtgc
      361 tccttaactt aatcgcccag aatacacaag gctatcatca gcttttaaaa atttccacgg
      421 caaaaatgtc tggcaagctt catatggatt acctctgcca acatttggaa gggatagcgg
      481 ttattattcc tagtaagggt tggagcgata cattagtggt cccttttgac tactatatcg
      541 gtgttgatca gtatactgat ttatctcata tggattctaa gaggcagctt atacccctaa
      601 ggacagttcg ttattttgcg caagatgata tggaaaccct gcacatgttg catgccattc
      661 gagataacct cagtctggca gagacccctg tggtagaaag tgatcaagag ttagcagatt
      721 gtcaacaact aaccgccttc tatcaaacac actgccctca agctctacag aatttagaag
      781 acttagtgtc aggaatctat tatgatttcg atacaaattt aaaattgcct cattttaata
      841 gagataagtc tgccaagcaa gaattgcaag acttgactga ggctggtttg aaggaaaaag
      901 gattgtggaa agagccttat caatcacgct tactacatga attggtcatt atttctgaca
      961 tgggctttga tgattatttt ttgattgtgt gggatttact tcgctttgga cgcagtaaag
     1021 gctattatat gggtatggga cgtggctcgg cggcaggtag tctagtggct tatgctctga
     1081 acattacagg gattgatcca gttcaacatg atttgctatt tgagcgcttt ttaaacaaag
     1141 aacgttatag catgcctgat attgatatcg atcttccaga tatttaccgt tcagaatttc
     1201 tacggtatgt ccgaaatcgt tatggtagcg accattcggc gcaaattgtg accttttcaa
     1261 cctttggccc taaacaggct attcgtgatg ttttcaaacg gttcggggtt ccagaatacg
     1321 aactgaccaa tctcactaaa aaaattggtt ttaaagatag cttggctact gtctatgaaa
     1381 agtcaatctc tttcaggcag gttattaata gtagaactga atttcaaaag gcctttgcta
     1441 ttgccaagcg tatcgaagga aatccaagac aaacgtccat tcacgcagct ggtattgtga
     1501 tgagtgatga taccttgacc aatcatattc ctctaaaatc gggcgatgac atgatgatca
     1561 cccagtatga tgctcatgcg gtcgaagcta acggcctgtt aaaaatggat tttttggggt
     1621 taagaaattt gacctttgtt caaaaaatgc aagagaaggt tgctaaagac tacgggtgtc
     1681 agattgatat tgcagccatt gatttagaag acccgcaaac gttggcactt tttgctaaag
     1741 gggataccaa gggaattttc caatttgaac aaaatggtgc tattaatctt ttaaaacgga
     1801 ttaagccaca acgttttgaa gaaattgttg ccactaccag tctaaataga ccaggggcaa
     1861 gtgactatac cactaatttc attaaacgaa gagaaggaca agaaaaaatt gatttgattg
     1921 atcctgtgat tgcttccatt ttagagccaa cttacggtat tatgctttat caagaacaag
     1981 ttatgcagat tgcacaggtt tatgctggtt ttacgttagg caaggccgac ttgttaaggc
     2041 gtgccatgtc taaaaaaaat ctacaagaaa tgcaaaaaat ggaagaagac tttattgctt
     2101 ctgctaagca cctagggaga gctgaagaaa cagctagagg actttttaaa cggatggaaa
     2161 aatttgcagg ttatggtttt aaccgcagcc atgcctttgc ctattcagct ttagcttttc
     2221 aattggctta tttcaaagcc cattacccgg ctgtttttta cgatatcatg atgaattatt
     2281 ctagcagtga ctatatcaca gatgctctag aatcagattt tcaagtagcg caagttacca
     2341 ttaatagtat tccttacact gataaaattg aagctagcaa gatttacatg gggctgaaaa
     2401 atattaaggg gttgccaagg gattttgctt attggattat cgagcaaaga ccatttaata
     2461 gcgtagagga ttttctcact agaactccag aaaaatatca aaaaaaggtt ttccttgagc
     2521 ctctgataaa aataggtctg tttgattgct ttgagcctaa ccgtaaaaaa attctggaca
     2581 atttggatgg tttactggta tttgttaatg agcttggttc tcttttttca gattcttcct
     2641 ttagttgggt agatacgaaa gattactcag caactgaaaa atattctttg gaacaggaga
     2701 tcgttggggt tggcatgagc aagcatcctt taattgatat tgctgagaaa agtacccaaa
     2761 cttttactcc catttcacag ttagtcaaag aaagcgaagc agtcgtactg attcaaatag
     2821 atagcattag gatcattaga accaaaacaa gtgggcagca aatggctttt ttaagtgtga
     2881 atgacactaa gaaaaagctc gatgtcacac tttttccaca agagtatgcc atttataaag
     2941 accaattaaa agaaggagaa ttctattact taaaaggtag aataaaagaa agagaccatc
     3001 gactgcagat ggtgtgtcag caagtgcaaa tggctattag tcaaaaatat tggttattag
     3061 ttgaaaacca tcagtttgat tcccaaattt ctgagatttt aggtgccttt ccaggaacga
     3121 ctccagttgt tattcactat caaaaaaata aggaaacaat tgcattaact aagattcagg
     3181 ttcatgtaac agagaattta aaggaaaaac ttcgtccttt tgttctgaaa acggtttttc
     3241 gataaaaatg gacaattagt gaagtatttt gcaaatgaat gtgctataat aagaccgtta
     3301 aattaattaa aggagtacca gaaaatgaaa cgtattgctg ttttaaccag tggcggcgac
     3361 gctcctggta tgaacgctgc tattcgtgca gtcgttcgta aagcaatttc agaaggaatg
     3421 gaagtttatg gtatcaaccg tggctatgca ggtatggttg atggcgacat tttcccactg
     3481 ggttcaaaag aagttggtga taaaatctca cgtggtggaa ctttcttata ctcagctcgt
     3541 tatccagagt ttgctcaact tgaaggtcaa ttggctggta ttgagcaact taaaaaacat
     3601 ggtattgaag gtgttgttgt tattggtggg gatggttctt atcatggcgc tatgcgcttg
     3661 acagagcacg gcttcccagc ggtaggtatc ccaggaacaa tcgataacga tattgcggga
     3721 actgactaca ctatcggatt tgatacagca gttaacacag cagttgaagc tattgataaa
     3781 cttcgtgaca cctcatcaag tcacggacgt acttttgtgg ttgaagtgat gggtcgcaat
     3841 gcgggtgata ttgctctttg ggcaggtatt gctagtggtg ctgatcaaat cattgttcca
     3901 gaagaagagt ttgatattga aaaagtggca tcaacaatcc aatatgattt tgaacacaaa
     3961 ggtaaaaatc accacattat cgtcttagct gaaggtgtta tgagcggtga agcttttgct
     4021 caaaaactga aagaagctgg cgataagagt gaccttcgtg tgacaaatct tggccacatt
     4081 cttcgtgggg gttcaccaac tgctcgtgac cgtgtcattg cttcttggat gggttcacat
     4141 gctgttgagc tacttaaaga aggcaaaggt ggtttggctg ttggtattca caatgaagaa
     4201 cttgttgaaa gcccaatcct tggtactgca gaagaaggtg ctttgttcag cttgactgaa
     4261 gaaggcaaga ttattgttaa taatccacac aaagctcgtt tagactttgc agctttgaac
     4321 cgttcattgt cacaataagt ttagttaaaa ttgttaaatc gtcaattatt gacaaaaaaa
     4381 taagggagtt tcagagtaaa atgaataaac gcgtaaaaat cgttgcaaca cttggtcctg
     4441 cggttgaaat ccgtggtggt aaaaaatacg gagaagatgg ctactgggct ggtcaacttg
     4501 atgttgaaga atctgcaaaa aaaatcgctg aattaattga agctggtgcg aatgttttcc
     4561 gtttcaattt ctcacatggt gaccacaaag aacaaggtga ccgtatggca actgttcgcc
     4621 ttgcagaaga aattgctcgt caaaaagttg gtttcctttt ggatactaaa ggtcctgaaa
     4681 tgcgtacgga attgtttgca gatgatgcta aagagtttag ctatgttact ggagaaaaaa
     4741 tccgtgtagc gactacacaa ggtattcaat caactcgcga tgttattgct ttgaacgttg
     4801 ctggtagcct tgacatctac gatgaagtag aagtaggtca tactattctt atcgatgatg
     4861 gtaaacttgg tttgaaagtg atcgacaaag atatcgcaac acgtcaattt atcgttgaag
     4921 tagaaaacga cggtattatt gctaaacaaa aaggtgttaa tatccctaac actaaaattc
     4981 ctttcccagc acttgcggaa cgcgacaatg ctgatatccg ttttggtctt gaacaaggac
     5041 ttaactttat tgcaatctca tttgttcgta cagcaaaaga cgttgaggaa gttcgtgaaa
     5101 tctgtcgcga aactggtaac gaccatgtgc aattatttgc taagattgaa aaccaacaag
     5161 gtatcgataa tcttgatgaa atcatcgaag ctgctgatgg tatcatgatt gcacgtggcg
     5221 acatgggtat cgaagtacca tttgaaatgg ttccagtttt ccaaaaaatg attatcacta
     5281 aagtgaatgc agctggtaaa gcagttatca cagcaacaaa catgcttgaa acaatgactg
     5341 aaaaaccacg tgcgacacgt tctgaagtat ctgacgtctt caatgctgtt atcgatggta
     5401 ctgatgcaac aatgctttca ggagagtccg ctaacggtaa atacccggtt gaatctgttc
     5461 gtacaatggc gactattgat cgtaatgcac aaactcttct taacgaatat ggtcgtttag
     5521 attcatctgc ctttccacgt accaataaaa cagatgttat tgcttcagct gttaaagatg
     5581 ccactcattc aatggacatc aaacttgttg tgactattac tgagacaggt aatacagctc
     5641 gtgcgatttc taaattccgt ccagatgcgg acatcttagc tgtaacattt gatgaaaaag
     5701 tacaacgtgc tttgatgatt aactggggtg ttattcctgt tcttgctgaa aaacctgcat
     5761 ctactgatga catgtttgaa gtagcagagc gtgttgctgt ggaggcaggc ttagttcaat
     5821 ctggggataa tattgttatt gttgctggtg ttccagttgg tactggtgga acgaatacta
     5881 tgcgtgttcg tacagtaaaa taaatgtgtt ataactattt acctaaaggt tagaaggcaa
     5941 gcttctaacc ttttttgata tcaaaaatat catgttaatt cactagtgtt aatttacttt
     6001 aatgagtcta agtttgtatt ttatacagaa tgagaaacgt ttattggtat tgatcaaaaa
     6061 ttgtttactt tttttctagc ttagaacatg gtataataag acaatagact aggagttaat
     6121 atggtaaagc gagattttat aagaaatata ttattgttat taattgtgat tattggggct
     6181 attttgttga gaatctttgt tttttcaact tttaaagttt ctccagaaac agctaatact
     6241 tatttaaaga gtggtgattt agtcacaatc aaaaaaaata ttcagcccaa atataaagat
     6301 tttgtggttt atagagttgg aaaaaaagat tatgtcagtc gagtcattgc tgttgaaggc
     6361 gatagcgtga cttatatgga cgatattttt tacctcaata atatggtaga gtcacaggct
     6421 taccttgaaa agatgaaagc acattacttg aatcatgcac cgtttggcac attgtataca
     6481 gatgatttta cagttgctac catcacagct gataagtatc aaaaagttcc taaggggaag
     6541 tatcttcttt tgaatgataa ccggaaaaat acgaatgaca gtcgtcgatt tgggttaata
     6601 aatgcctcgc agattaaagg tttagtgacc tttagagttt tgcctctcag cgattttgga
     6661 tttgtagaag tagagtagtt gtcaggctac tctacttttt ttataattag actataccaa
     6721 ttttatgttt gacaagacat tgtggatatt atatactgta aacataatgt ttttatcggt
     6781 ataattagac tataccaatt attgaaatta tgagagaaag tagcaagtta acgaacttgt
     6841 taaggtaatt aagatgtgtg gaattgttgg agttgttgga aatcgcaatg caacggatat
     6901 tttaatgcaa ggccttgaaa agcttgaata ccggggttat gattcagcag gaatttttgt
     6961 ggctaatgcc catcaaacaa acttgattaa atcagtgggg cggattgctg atttgcgtgc
     7021 caagattggc attgatgttg ctggttcaac agggattggt cacacccgtt gggcaacgca
     7081 tggccaatca acagaggata atgcccatcc tcacacgtca caaactggac gttttgtact
     7141 tgttcataat ggtgtgattg aaaattacct tcacattaaa acagagttcc tagctggaca
     7201 tgattttaag gggcagacag atactgagat tgcagtacac ttgattggaa aatttgtgga
     7261 agaagacaag ttgtcagtac tggaagcttt taaaaaagct ttaagcatta ttgaaggttc
     7321 ctacgccttt gcgttaatgg atagccaagc aactgatact atttatgtgg ctaaaaacaa
     7381 gtctccattg ttgattggac ttggtgaagg ttacaacatg gtttgttcag atgccatggc
     7441 catgattcgt gaaaccagtg aatttatgga aattcatgat aaggagctag ttattttaac
     7501 caaagataag gtaattgtta cagactacga tggtaaagag ctgatacgag attcctacac
     7561 tgctgaatta gacttatctg atattggcaa agggacttat cctttctata tgctgaaaga
     7621 aattgatgag caaccaaccg taatgcgtca attaatttca acttatgcag atgaaactgg
     7681 taacgtacag gttgatccag ctatcattac ctctatccaa gaggctgacc gtctttatat
     7741 tttagcggca gggacttcct accatgctgg ttttgcaaca aaaaatatgc ttgagcaatt
     7801 gacagataca ccagttgagt tgggcgtggc ttctgagtgg ggttaccaca tgcctctgct
     7861 tagcaagaaa ccaatgttta ttctactaag ccaatcagga gaaaccgcag atagtcgtca
     7921 agttttagta aaggcaaatg ctatgggcat tccgagtttg acagtaacta acgttccagg
     7981 atcaacctta tcacgtgaag caacatacac catgttgatt catgctggac ctgaaattgc
     8041 tgttgcgtct acaaaagctt acactgcaca aattgctgcc cttgcctttt tggctaaggc
     8101 agttggtgag gcaaatggta agcaagaagc tcttgacttt aacttggtac atgagttgtc
     8161 attggttgcc caatctattg aggcgacttt gtctgaaaaa gatctcgtgg cagaaaaggt
     8221 tcaagctttg ctagctacta ctcgtaatgc tttttacatc gggcgtggca atgattatta
     8281 cgttgcgatg gaagctgctt tgaaattaaa agagatttct tatattcaat gcgaaggctt
     8341 tgcggctggt gaattgaaac atggcaccat ttcattgatt gaggaggaca cgccagtaat
     8401 cgctttaata tcgtctagtc agttggtttc ctctcatacg cgtggtaata ttcaagaagt
     8461 tgctgcccgt ggggctcatg ttttaacagt tgtggaagaa gggcttgacc gtgagggaga
     8521 tgacattatt gtcaataagg ttcattcttt cctagccccg attgctatgg tcattccaac
     8581 tcaactgatt gcttactacg cttcattaca acgtggactt gatgttgata agccacgtaa
     8641 tttggctaaa gctgtaacag tagaataaat gattataatg gataaggcaa ccatctgtta
     8701 ggtggttgct ttttgttttt aggagaatag cagagcttag aaaatggcct tgagaagaat
     8761 agtcatcttc tgatgagaac gtattcatct tgtcaaatca ttgattctaa agtaaaatgg
     8821 taacattaag aaatgtttag gagtgtaaga tgtcattacc aaattgtttg caatgccagt
     8881 ctgaatatgt ttacgaagat ggtcttttat taatctgtcc aatgtgtgct tttgaatgga
     8941 caccaggcga agaagcgact gaagaagaag gacttgttgt tttggatagc aatggtgtcc
     9001 gactgtctga tggtgatacc attactgtgg ttaaagattt gaaagtaaaa ggcgctccaa
     9061 aagacctgaa acaaggaact cgagttaaaa atattcgcct ggtggaaggt gatcataaca
     9121 ttgattgtaa gattgatggg tttggcgcga tgaaattgaa atcagaattt gtcaagaaaa
     9181 tctaataata atacgcaacc agctgatctg gttgtatttt tataaaaaaa tctctcccca
     9241 aatacgttca aatttggtat aatagtaaaa tatttagttt taggaggcaa catgacctat
     9301 attcagcaag ttttacccag cttattagat ggtgccttgg tgaccttaca agtattcttt
     9361 attgttatca ttctttctat acccttaggg actattttag ctttcttgat gaagattccc
     9421 tttaaaccgc tacagtggtt tttgacccta tacgtgtgga tgatgcgagg gacaccatta
     9481 ctacttcaat tgattttttt ctattatgtt ttgccaagtg tggggattag ttttgatcga
     9541 atgccagctg ctattttggc gtttactttg aattatgctg cctactttgc tgaaattttt
     9601 agaggtggga ttgaggctat tccaaaaggt caatatgaag cagctaaagt attaaagtta
     9661 aaacctcttc aaaccattcg ttatattatt ttgcctcaag tgtttaaaat tgtgttacca
     9721 agtgttttca atgaagtcat taatttggtc aaagattctt ctcttgtcta tgtactcggt
     9781 gtaggagatc ttttattagc aagtaagacg gcagccaata gggatgcaac cttagcccct
     9841 atgtttatcg ctggtcttat ctatttgctt ttaattggac tggtcacgat tatttcaaaa
     9901 caagttgaaa aacggtttaa ttattatcag taaggaggct tcatgttaga actcaaaaat
     9961 atttccaaac agtttggtca aaaaaccatt tttgatggct ttaatctaac cgttcaagac
    10021 ggagaagtgc tctctttggt aggcccatca ggtggcggta agacaacact cttacggatg
    10081 ttggctggtc ttgaatctat tgattcaggt caagtctttt acaatggcga agatgttggt
    10141 attgatcatt tggaaaatcg taacttgctt ggatttgttt tccaagattt tcaattgttt
    10201 cctcatctta ccgttttaga caacctaacg ctgtctccaa caattacaat gggcaagcaa
    10261 aaggctgacg ctaaagaaaa ggctttggat ttactggcac gtttaggatt aaaagaacat
    10321 gcccaagttt atccctattc gttatctgga ggtcaaaaac aacgggttgc tttggcaaga
    10381 gccatgatga ttgcccctca aattatcggt tacgatgaac cgaccagtgc tctagatcct
    10441 gaattacgtc agacggtaga agccttgatt gttcaaaacc gtgaaatggg aataactcaa
    10501 attgtagtca ctcacgatct tgtttttgcc gaagccatct cagatcgtat tattagggtt
    10561 aatcctaaat agaagaggac ccaaaagatg attataaaaa aaagaaccgt agcaatttta
    10621 gccatagcta gtagcttttt cttggtagct tgtcaagcta ctaaaagtct taaatcagga
    10681 gatgcttggg gagtttacca aaagcaaaaa agtattacag ttggttttga caatacgttt
    10741 gttcctatgg gctataagga tgaaagcggc aaatgcaaag gttttgatat tgatttggct
    10801 aaagaagttt ttcaccaata tggactcaag gttaactttc aagctattaa ttgggacatg
    10861 aaagaagcag aactaaacaa tggtaaaatt gatgtaatct ggaatggtta ttcaataact
    10921 aaggagcgtc aggataaggt tgcctttact gattcttaca tgagaaatga acaaattatt
    10981 gttgtcaaaa aaagatctga tattaaaaca atatcagata tgaaacataa agtgttagga
    11041 gcacaatcag cttcatcagg ttatgactcc ttgttaagaa ctcctaaact gctgaaagat
    11101 tttattaaaa ataaagacgc taatcaatat gaaaccttta cacaagcttt tattgattta
    11161 aaatcagatc gtatcgatgg aatattgatt gacaaagtat atgccaatta ctatttagca
    11221 aaagaagggc aattggagaa ttatcggatg atcccaacga cctttgaaaa tgaagcattt
    11281 tcggttggac ttagaaaaga agacaaaacg ttgcaagcaa aaattaatcg tgctttcagg
    11341 gtgctttatc aaaatggcaa atttcaagct atttctgaga aatggtttgg agatgatgtt
    11401 gccactgcca atattaaatc ttaaaaagga cacttgtcct ttttttgttt aacaggtgag
    11461 atctagcaga caaagtcagc tagatgattt aata
//
LOCUS       DAUGHY010000046        11058 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.046, whole genome shotgun sequence.
ACCESSION   DAUGHY010000046 DAUGHY010000000
VERSION     DAUGHY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11058
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.046"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            407..535
                     /locus_tag="VH752_001357"
     CDS             407..535
                     /locus_tag="VH752_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEQ8289703.1"
                     /translation="MAFGENGPRKKTTFEKVTMFVVILMVLVTVGGLIASALSVLM"
     gene            592..1905
                     /gene="obgE"
                     /locus_tag="VH752_001358"
     CDS             592..1905
                     /gene="obgE"
                     /locus_tag="VH752_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEQ8289704.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFRVDEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDMPEAQENLKAFKKKLAAQYDEFDDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDEADLVDEEAYYGFAETEKDFEITRDDDATWVLSGEKLERLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDPVRIGKFEFEFVD"
     gene            1974..2132
                     /locus_tag="VH752_001359"
     CDS             1974..2132
                     /locus_tag="VH752_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289705.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(2137..2832)
                     /locus_tag="VH752_001360"
     CDS             complement(2137..2832)
                     /locus_tag="VH752_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEQ8289706.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISALLFGKQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILVTLIFYGILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDASQYAVVICFATFLINDSYTFYSWL
                     MLQRYQEKKVKKG"
     gene            3057..4457
                     /locus_tag="VH752_001361"
     CDS             3057..4457
                     /locus_tag="VH752_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ8289707.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSDAPEDVKAARIAELFQTKSFLDPSVLGVYPAELVSILEEAD
                     LLPQYSADELEVIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDPLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRI
                     TFIEDHLIQLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            4466..5560
                     /locus_tag="VH752_001362"
     CDS             4466..5560
                     /locus_tag="VH752_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEQ8289708.1"
                     /translation="MVDLGFSLYPERYDVTKSKAYIDLCHSYGAKRLFMSLLQLAPAD
                     HQMFHCYAELIAYANQLGIRVIADVSPSFISQAGWSDQLIERAHAFGLAGLRLDEALP
                     LAEIVTLTRNPFGLKIELNMSTDKQLLMSLLATDAEQSNIIGCHNFYPHEFTGLSWQH
                     FKDMSRFYHEHDIETAAFITAQSASEGPWLLAEGLPTVEDHRHLPIGLQVELMKAIGT
                     IDNILISNQFISEEELAACTQALARPVTTIKVRPIIDLTEVEEKIIGYPHCYRGDVSD
                     YVIRSTMPRLVYAQESIAPRDQSKEVKRGSIIIDNDRYHRYKGELQITLKNFTVSSKA
                     NVVAEVREDYLSLLDDLRPWQEFCLEIDPS"
     gene            5666..7660
                     /locus_tag="VH752_001363"
     CDS             5666..7660
                     /locus_tag="VH752_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ8289709.1"
                     /translation="MLSKKELAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKEL
                     ISSLPEHGAHIISKQGRGYCLEIDHSMAFDIFWQESVTSKKRLADVTQVEESVDREDY
                     VLHKFFFEDAVQDFEELCQELYISRTTLKHVLAGIKERIIPYQLELEITHQHIQIRGK
                     EEDIRHFIMDYFFVTSFDNTLSTMVGNTFLEGINFAEIMIIVLDECRDAKLKLSDFVM
                     NNLVLHIALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYRVEEVMGIRFPKEEA
                     NYIALHLKVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAH
                     MMPLTTRLENHIQLTNPLTEEIKSQYPEIFTLTKQTFSDLLVCQKNDVSDDEWAYISL
                     HLMAAIERYSNRYKLRVLVVCATGYGSAMMLKNRLEKEFEGRLRIVDVISYYEITEER
                     LKTVDLIISSISLANLMFLTPVITVSVFLSNQDIETIRQFIGEQEGIKKEVSLPSQMS
                     LAKAEQLLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTETFVEDFYHQMVLREN
                     YSPVIYGEVLAFPHPANPMTYSEQVVVAICREPLEWDEAHQAVHFVFLLSPSKGHNHR
                     LKYVSPGLASFVNQVELQQALLEEPNYSKFMTVFTPLIHD"
     gene            7683..7994
                     /locus_tag="VH752_001364"
     CDS             7683..7994
                     /locus_tag="VH752_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ8289710.1"
                     /translation="MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            7997..8332
                     /locus_tag="VH752_001365"
     CDS             7997..8332
                     /locus_tag="VH752_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ8289711.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAIELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRGES"
     gene            8329..8778
                     /locus_tag="VH752_001366"
     CDS             8329..8778
                     /locus_tag="VH752_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEQ8289712.1"
                     /translation="MIISAEGDVSVHHCFEAMLISLQEDHRQNTLRQLSRDDIKAGIN
                     YVKHYSKYKERTVRVLITALEAPQLYTACFSSNHGIKELAYRLEALGENRTRITYSYD
                     YHPLDIFQKANHFIVGKLFKKSLERQSQAQLAALIQYAKQLQQPSSL"
     gene            8956..10335
                     /locus_tag="VH752_001367"
     CDS             8956..10335
                     /locus_tag="VH752_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ8289713.1"
                     /translation="MSHFSDKFMDISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGGSSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGVFNINEPITFGMPIVLNPTFVIPWLIVPPICASIA
                     YFATAIGLIPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            10332..10484
                     /locus_tag="VH752_001368"
     CDS             10332..10484
                     /locus_tag="VH752_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289714.1"
                     /translation="MTEKNGATFLMAVGFFLIAISPMVGNKYSHIVTGLALTGLGYYL
                     TKHKRP"
     gene            complement(10672..11058)
                     /locus_tag="VH752_001369"
                     /pseudo
     CDS             complement(10672..11058)
                     /locus_tag="VH752_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468698.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3289 a         1990 c         2416 g         3363 t
ORIGIN      
        1 attcttacat ttttgaacaa tggttacccc atacctatca atcaagcgaa tcatatattt
       61 gagatcggat tctgccatat caaacttttt tgatatggat ttaatggact ctcccatttg
      121 ccgtaactcg taaatcttaa ctttcgtttc ttgattaaat ttcataaaaa aatagcaccc
      181 caaccgttag actttgtgtc taacttttgg ggtgcagttc accagacgag gaggttttta
      241 tctgtaacat tattttgcaa atgctaatgt atcatattca tatgaaattc acaagataca
      301 atactcagta atagtctaca tagcgtaaat attcatctgg tatttgccta aaacgatctt
      361 tcatgttaat atataagaag aaaaatcaat agatgaggta tttaacgtgg catttggaga
      421 aaatggacct cgcaaaaaaa caacctttga aaaagtaaca atgtttgttg ttatcctaat
      481 ggttcttgta actgttggcg ggcttattgc gagtgcccta tcagtattaa tgtaaggaaa
      541 cgctgaaagg cgttttttta aagtaaaaga aagttagaga ataaaaaaag tatgagtatg
      601 tttttagata cggcaaaaat cagtgtccaa gcaggccgtg gtggcgatgg tatggtagct
      661 tttcgtcgtg aaaagtacgt cccaaatggc ggtccttggg gaggagacgg tggtaaaggt
      721 ggctcagtta tcttccgagt ggacgaaggt ttgcgtacct tgatggattt ccggtataat
      781 cgtaaattca aggcgaaatc tggtgaaaaa ggtatgacca aaggcatgca cggccgtggt
      841 gcagaagatt tgattgtctt tgttcctcaa gggacaacgg ttcgtgatgc tgaaactgga
      901 aaagtaatta cagacttagt agaacatggt caagaagtgg tcattgctaa gggtggtcgt
      961 ggtggccgtg ggaatattcg ttttgcaact cctcgtaatc ctgcgccaga gattgctgaa
     1021 aatggtgagc caggtgagga acgtcagtta gagttagaac tcaaaatctt agctgacgtt
     1081 ggcttggtcg gattcccatc tgttgggaaa tcaaccttgt taagtgttgt gtcatcagct
     1141 aaaccgaaaa ttggtgccta tcattttacc actattgttc ctaatcttgg aatggttcgt
     1201 accaagtcag gtgatagctt tgctatggca gatctgccag gtttgattga aggtgctagc
     1261 caaggggttg gtttaggaac tcaattcctc cgtcatatcg agcggacacg ggttattctt
     1321 catgtgattg acatgtctgc tagtgagggt cgtgatcctt acgaagatta tgtttctatt
     1381 aataacgagc tggaaaccta taatttgcga ttgatggagc gcccacagat tattgtggct
     1441 aataaaatgg acatgccaga ggctcaagaa aacttgaaag ccttcaagaa aaaattggct
     1501 gcccaatatg acgagtttga tgacctacca atgattttcc ctatttcaag cctagcacat
     1561 caaggcttag agaacttgtt agaagcaaca gcagaattac ttgctaagac ggatgaattc
     1621 ttattatatg atgaagctga tctggtggac gaggaagctt actatggctt tgcagaaact
     1681 gaaaaagact ttgagattac ccgagatgat gatgctactt gggtcttgtc tggtgagaaa
     1741 ttggaacgtc tctttgtcat gaccaatatg gaacgtgacg aatccatcat gaaatttgcc
     1801 cgtcaattgc gcggtatggg ggttgacgaa gcccttcgtg aacgtggagc aaaagacggg
     1861 gatcctgttc gtattggcaa atttgaattt gaatttgtgg attaatgtgg tctttagaaa
     1921 tggcattgcc atagcaacct tgtgttttat ctttaagatg gaggtttctg attatgggtg
     1981 ataaaccgat atcctttaag gataaggatg gaaattttgt atcggcagca gatgtttgga
     2041 atgctgagaa attagaagaa ttgtttaatc ttttaaatcc caatagacgt ttgcgcttag
     2101 aacgtgaaaa attgaaaaag gatgaggctt aggccctcat ccttttttga cctttttttc
     2161 ttggtaacgt tggagcatta gccaactata aaaagtgtaa ctatcattaa taagaaaggt
     2221 ggcaaagcag atgaccacag catattggct ggcatctgtt tgagcagcat gcatccataa
     2281 gagaatgagg actagatcat tgagtccata agccaaagca aaataagggc ttcgcttata
     2341 gctgagataa acggctgaga aagaagtggc aatcgataaa gtggagacaa gtaaataggc
     2401 tgtttgaata gaagccaaga tgccgtagaa aataagagtg accagtatag taaaaacgaa
     2461 aagacatctt cgatcagtgg gtgttaatcg tgaaatagtc acttgtgatt ttttcccttc
     2521 aaaaggatgg tttaaccaag taaaaagtga aaaaatagtc atagggaggg tcatgaaaag
     2581 ataggtcatt aactctccat agtaactgtt tcgtaatgaa agataggcat agataatgga
     2641 aaaaataatg acgagccctt gaccaatagg attagcttta gcactaaaaa tgagagaggt
     2701 cacaccaatc aaagaagcta ttaaagctaa gggagcttgc ttaccgaata aaagggcgct
     2761 aattaaaatg gccaagacag agcttaacca aagtgcccac tcggtttttg taaagtagtt
     2821 taaataagcc atacccctat tcaaacatag gctaaggcat ctgtcaatag gggcttggtt
     2881 ttgataagaa gtagcagggc aggttagata ggctctttaa gataataaaa tatgtcaagc
     2941 tttaaatcac ttattttccg tgatgataag gaaaaaacag attttttaaa gcgtttacat
     3001 cacctgtttt tcttcttata ataaaaagag aagtgagcaa agaaaggaca aaggatatga
     3061 aacaaagaaa acaccgttat caatttccag atggcttttt atggggaagt tccacatctg
     3121 ggcctcaaag cgaaggaacg gttcctggag atggcaaagg acctagcaac tgggattact
     3181 ggtttagtat tgagtcagct aagtttcatc accaaattgg tccagaaaaa acctcaacct
     3241 tttatgaaaa ctataagggt gacattgccc ttttgaaaga aacaggccac acgatttttc
     3301 ggacctcaat ccagtggtct cgattgattc cagaaggagt cggggaggtt aatccaaagg
     3361 cggtgacatt ttaccgagaa gtatttcaag acattattgc tcaaggtatt aaattgattg
     3421 ttaacctcta tcattttgac cttccctatg ccttgcaaga aaaaggtggc tgggaaaaca
     3481 aagctactgt ctgggcttat gaaacctacg caaaaacctg ttttgagctt tttggtgatt
     3541 tagtaaacac ttggattacc tttaatgaac cgattgttcc agtcgaatgt ggctatttag
     3601 gttattatca ctacccttgt aaagtagatg ccaaggcagc agttcaagtg gcttacaaca
     3661 cacaactggc cagttcactg gcggttaaag cttgtcataa gcttcatccc gaccacaaga
     3721 ttagtattgt gcttaacatg acacctgctt atcctcggag tgatgctcct gaagatgtca
     3781 aggctgctag aatcgcagag cttttccaaa ccaaatcctt cctagatcca tcagttttgg
     3841 gtgtttaccc agcagagtta gtttccattt tagaggaagc tgatttatta cctcaatata
     3901 gtgctgatga attagaggtt atcaaaaaca atactgttga ttttttggga gttaattatt
     3961 atcagccttt gcgggttcag gcacctagta agtcacagca agagggggac ccccttatct
     4021 tagatattta ttttgaacct tatgatatgc ctggtaaaaa agtcaatcct caccggggtt
     4081 gggaaattta tgaaccagga ctttatgata ttgcccttaa tttaaaagaa cactatggta
     4141 acatcgaatg gctagtcacg gaaaatggta tgggtgtcga aggtgaagaa gcttttttag
     4201 cagatggcca gatacaagat gactaccgca tcacctttat cgaggatcat cttatccagc
     4261 ttcataaggc tttgggagaa ggagccaact gcaaaggcta cctcttgtgg acttttattg
     4321 attgctggtc ttggttaaat gcttataaaa accgatatgg tttggtagct ttggatttag
     4381 agagtcaaaa gcgaaccata aaaaaatcag gttactggtt taaggcgttg agcgagtcaa
     4441 atggttttga taaataggag gcaatatggt tgatttagga ttttctctat accctgagcg
     4501 gtatgatgtt accaagagta aagcttatat tgacttatgt catagctatg gagccaagcg
     4561 cttgtttatg agtctcttac agctggcacc agctgatcac cagatgtttc attgctacgc
     4621 agagctcatt gcttatgcaa atcaattagg cattcgagta attgctgacg ttagtccaag
     4681 ctttattagc caagctggtt ggtcagatca gcttattgaa cgagctcatg ccttcgggtt
     4741 agcgggctta cgcttagacg aggctctccc tctagcagag attgtaactt taacaagaaa
     4801 tccctttggg cttaaaattg aacttaatat gagcactgat aaacaattat tgatgtcatt
     4861 gctagcgaca gatgcagaac agagcaatat tattggttgc cataattttt atccacatga
     4921 atttaccggt ttgtcatggc aacattttaa ggatatgtca cgtttttatc atgagcatga
     4981 tattgaaacg gctgctttta ttaccgctca gtcagcatct gaaggccctt ggctactggc
     5041 agaaggcttg ccgacagtgg aagaccatag gcacttgccc attggcctgc aagtagaatt
     5101 aatgaaagcg ataggaacca ttgataatat cctcatatct aaccaattca tttcggaaga
     5161 agaattggca gcatgtactc aggctttagc aagaccagtc acgacgatca aggttaggcc
     5221 tatcatcgat ttgacagaag ttgaggagaa aatcattggc tatccgcatt gttatcgtgg
     5281 cgatgtatct gattatgtga ttcgatcaac catgccacgt ctagtttatg ctcaggaatc
     5341 gattgctcca agagaccaat ctaaggaggt caaacgaggt agcattatta ttgacaatga
     5401 ccgctatcat cgatataagg gagaactgca aattactttg aaaaatttta cggttagttc
     5461 aaaagctaat gtggttgctg aggtcagaga ggactatctt agtttgttag atgatttgcg
     5521 accttggcag gaattttgtt tggagattga cccatcatag attcttttca caatcaatgc
     5581 ggaaaatgat ggtcaccttt ttcaggaaat atttggtaca ctaaaagagg gtagagagcg
     5641 ttttctaaag tagtaaggag gactaatgtt atcaaaaaaa gaactggcca tcataacatt
     5701 tctaatccat cacaaagagc agtttgtctc tagtgcaact ctggctgagg ttattggtat
     5761 gagtgaccgt acggttagga aatacctcaa agaactgatc agtagccttc ctgaacacgg
     5821 agctcacatc atttctaaac aaggtcgagg ttactgctta gaaattgacc attctatggc
     5881 atttgacatc ttttggcaag aaagtgtgac gtctaaaaaa cgtttagcag acgtgactca
     5941 agtggaagaa tcggttgatc gagaagatta tgtgcttcat aaattctttt ttgaagatgc
     6001 cgttcaggat tttgaagagc tttgtcaaga attgtatatc agtcggacaa ctttaaaaca
     6061 tgttttagca ggcattaagg agcgcataat accttaccag ttagagcttg aaataactca
     6121 ccaacatatt caaataagag gtaaagaaga ggacattagg cattttatca tggactattt
     6181 ttttgttact agttttgata ataccttgtc caccatggta ggtaatacct ttcttgaagg
     6241 tattaatttt gcagaaatca tgattattgt cttggatgaa tgtcgggatg ctaagttgaa
     6301 actttctgat tttgtcatga ataatctagt cttgcatatt gccctaatgg ttcagcgtat
     6361 tcgatcgggg tgtccattag aactgttttc cattccaata gctattcgtc agtctgatga
     6421 gtatcaagta gctctgcgca ttttgtatcg agtggaagaa gtgatgggga tacgttttcc
     6481 taaagaggag gctaattata ttgcactgca tctaaaggtc aaacattctg ttggtaagca
     6541 ttggcaagat gataataccg atgaaaagtt gcaggaccat ttaaaagcat gtattgccaa
     6601 gattagtcaa ttgacaggca tgactttaga aacagatacc aatttatttc aaggtttatt
     6661 agcccacatg atgcccttga ccacacgtct tgaaaaccat attcagctga ctaatccttt
     6721 aactgaggaa attaaaagcc agtatcctga aatatttacc cttaccaagc agaccttttc
     6781 ggatttgctt gtatgtcaaa agaatgatgt ctcagatgat gaatgggctt atattagttt
     6841 gcatttaatg gcggccattg agcgttattc aaaccgttat aaattgaggg ttctagtagt
     6901 ttgtgccaca ggttatggta gtgccatgat gttgaaaaat cgcttggaaa aggaatttga
     6961 aggacgtttg cgtattgtcg atgttatcag ttactatgaa ataaccgaag agcgactgaa
     7021 aaccgttgat ttgattattt cctctatcag ccttgccaat ctgatgtttt taacaccagt
     7081 gattactgtc agtgtttttt tgtcaaatca ggatattgaa actattcgac aatttattgg
     7141 agaacaagaa ggtatcaaga aagaggtcag tttaccgtca cagatgagtt tagcaaaagc
     7201 agagcagctt ctaacaggtg tttttagccc taatcgtttt ctttatttag acgagaaaat
     7261 ctcaaaggaa gacttgcttt tacggatgat tgcttgtctt gacgaggcgg gaacagaaac
     7321 gtttgtagag gacttctatc atcaaatggt actgagggaa aattacagcc cagtcatcta
     7381 tggagaagtg cttgcctttc cgcatcctgc taatcccatg acttattctg aacaggtagt
     7441 agtggctatt tgccgagagc ctcttgagtg ggacgaggcg catcaagctg ttcattttgt
     7501 ctttctatta tccccgtcta aagggcataa tcataggtta aaatatgtat caccaggttt
     7561 ggcatctttt gtcaatcagg tggagcttca gcaagcgtta ctagaagaac caaattattc
     7621 caaattcatg acagtgttta ccccacttat tcatgattaa aacgattaaa aggagaaacc
     7681 ttatgaaatc tattatgtta gtttgtaatg ccggtatgtc aactagtatg ttggtgacta
     7741 agatgcaaaa agcagcccaa gcgcgtgacc ttgaagtttc tatttgggca gttcctgtga
     7801 gtgaagctga caacgaagtg gctgctaata gcattgacgt tctcttgtta ggtccacaag
     7861 tcaaattctt actaaaggat tttaaagata agtttgaacc agatattaaa gtagatgcca
     7921 ttaacatggc tgattatggt cttatgaacg gtgaaaaagt ccttgaaaca gccctagcga
     7981 tgatggagga gtaggcatga cggaaccatc caacttagaa gctattatgg gcttaatcat
     8041 gtatggaggc gaagccaagg gcaaggccgt agaggctatt catgcagcac gagatgggca
     8101 gtttgaggag gctcagcact tacttcaaga agcgacaaac tcgttaaata ttgcccataa
     8161 atcgcagaca catttgctct cccaagaagc agctggtgat gcgattgaat tgtctttatt
     8221 gatggtgcat gggcaagatc acatgatgac tgccttagcc tttattgatt tagccaagga
     8281 attggttacc gtttacgaca aaatgtcagc aatacagagg ggtgagtcat gatcatttca
     8341 gcagaaggag atgtttccgt tcatcattgt tttgaagcca tgctgatatc gctccaagaa
     8401 gatcatcgac aaaatacctt gagacagttg tcaagagatg atatcaaagc ggggataaac
     8461 tatgttaaac attatagtaa atacaaagag cgtacagtga gggtgcttat tacggcccta
     8521 gaagctccgc aattatatac tgcttgtttt agttccaatc atgggataaa ggaattggct
     8581 tatcgattag aggctctggg ggagaatcgg actagaatta cctattctta tgactatcat
     8641 cctctagata tttttcaaaa agcaaatcat tttattgttg gcaaactgtt caaaaaaagc
     8701 ctagaacgcc aatctcaagc tcagttagca gcccttatcc aatatgctaa acagttgcag
     8761 caaccatcgt ctttgtgaag attattcaca gaccttggat gagagagaag ggttcatttg
     8821 gataagagtc gattaccggt tatctctaag catcacgttt aacaaggatg attttgagat
     8881 aattgtgtta ggcagacacc gctgctttta aggttacctt tgttacatta aaaaaataaa
     8941 aagaggagaa gatttatgtc gcatttttct gataagttta tggatatttc aggaaaaatt
     9001 gggtcacaac ggcatttagt agctattcgt gattccttta tttcaatgat gcctattacc
     9061 atggcaggtt cagttgctgt cttattgaac gtttttttac gcgatattcc aaacaatatg
     9121 ggatggacag ggtttgcaaa agctatgcaa cctgttatcg acatcaatgg ctatgtttat
     9181 tttgggacaa ttggtatcat ggcgctcttt tttgcctttg ctttcggtta caatttagct
     9241 gttatgcata agattaatcc attggcagga ggattgattt cttttggctc atttattgcc
     9301 accctacctc agactttaac catttcgaca ccacttgaaa atgcctctgc taacctaatt
     9361 agcaatttaa aagaaatggg gctttcagtc gttactgcgg gtggaggttc aagtattgaa
     9421 actagtcaat ggggagcaat agctctaaag tatgtcggtg ctacaggctt gttcacagct
     9481 ttaattattg gctttttatc aagttttgtg tatgcggctt tgaccaaacg taatattacc
     9541 attaccttac cagataatgt accacctgcg gttaataaag cctttgctgc cattattcca
     9601 ggaacagttg cgatttacgc ttcggcaatt ttcgcctacc tcatctttgc attaacaggt
     9661 tcttccctaa gtgatgtcat ttcaacctat atccaattac cattactagg cttatcacag
     9721 ggtattggtt cagttatctt gttaaccttc ttagttcaac tgctctggtt cttcggcttg
     9781 catggtcata atgtcttagc cccagtgatg gatggtattt atatggtagc tttaacagaa
     9841 aatacagctg cttacaatac agcgcatagc gctgctaatt taccatacct ttggacacgc
     9901 gggtcctttg atgcttatgc tcaaatgggt ggttcaggag taaccttggc attgattatt
     9961 gctatcttca tattctcaaa acgagaagag cataaaacca ttgccaaatt gtccgcacca
    10021 atgggtgttt tcaatatcaa cgaaccaatt acatttggga tgccaattgt tttgaatcca
    10081 acctttgtga ttccatggct aattgtgcca ccaatttgtg ctagcatagc ttattttgct
    10141 actgcaatag gtttgattcc accggtattc ttatcagtac cttggattac ccctgttgga
    10201 ctttatgctt acctagctac tggaggtaat attatggcag gtttggtttc actgtttaac
    10261 ctctttgttg ccttccttat ctgggcacca tttgtcattt tggccaataa agaaaaagcg
    10321 agtgaccttg catgacagaa aaaaatggag caactttttt aatggctgtt ggcttttttc
    10381 tcattgccat tagtccgatg gttggtaaca aatactcaca catagtgacg ggactagctc
    10441 ttacgggatt aggttattac ctcactaaac ataaacgccc ataaaaaagc ataggggaca
    10501 ctcttatgaa ggagagagtc ataagagtat ccctattttg ctggtttgaa taataacagt
    10561 aaccaaaaaa ggcaaggaaa cctgtttcct tgcctcagat tgaagacaaa catcgatttg
    10621 gtgtttgtct tttttagtgt ttttagataa aaatggctgt ttccgtgctt aaaccacctc
    10681 atctgaacaa aataaaaagg ctctcccacc atccttttga cccgtttttt gagatttaag
    10741 catgccaaag tcagtccaac cttatcttcc attttggact tgcctttctc tctcgtatag
    10801 cggagattgt ggtattcctt ggctgtgcca aacagtcgtt caatcgtctc tttccgatgc
    10861 tggtagagct ccttcatccc cctttgatga cgaatgtcct cgcactgttc caaatagtct
    10921 ttccaaatat gccgtgtgac aactttttgg cggttcttac tggtcgtaca tacagataac
    10981 aggggacaat tggcacaaat tgcagggtca cttttgtatt ctcggtagcc ttctctggtc
    11041 gtagtgcggt aggtcaag
//
LOCUS       DAUGHY010000047         9755 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.047, whole genome shotgun sequence.
ACCESSION   DAUGHY010000047 DAUGHY010000000
VERSION     DAUGHY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9755
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.047"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..761)
                     /locus_tag="VH752_001370"
     CDS             complement(<1..761)
                     /locus_tag="VH752_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEQ8289715.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAEGSAAPL
                     TSEQRQQEIEKIKQEIQAKYPKLTEEKYHSYGVFGRKYLDYDKYQKGLEEYTQEALKK
                     IVELIEKGATQGPAGPKGETGPAGPAGPEGKPGKAGEKGDRGEKGEAGIQGPQGEKGD
                     TGAQGPQGEAGAPGEKGEKGDRGETGAQGPVGPQGEKGETGAQGPQGEAGKPGEQGPQ
                     GEAGKPGEKAPEKSPEGEAGQPGEKAPEKSPEVTPT"
     gene            1143..1604
                     /gene="nrdI"
                     /locus_tag="VH752_001371"
     CDS             1143..1604
                     /gene="nrdI"
                     /locus_tag="VH752_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ8289716.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGEVEILTTPLGEFIAAHGNAQHCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFPLLGDFELRGTPDDISRLAQVIMEASSRHSSNS"
     gene            complement(1999..2817)
                     /locus_tag="VH752_001372"
     CDS             complement(1999..2817)
                     /locus_tag="VH752_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEQ8289717.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPSIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYQEG
                     VAILSKQPIHVSDILVSAMADETDYHTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTDQDGYQVMMGSPLNLQDSHKVADHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLDWR"
     gene            complement(2900..5086)
                     /locus_tag="VH752_001373"
     CDS             complement(2900..5086)
                     /locus_tag="VH752_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ8289718.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHDSAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGAFASGLAFVLINRI
                     TGAFYGVSSAMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEILTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYIVYALVQGASFAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSILFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDRNTQATPTQVADSNSQVVQIINLLGGSGNIDDVDACMTRLRVTVKDP
                     AKVGAEDDWKKAGAMGLIQKGNGVQAVYGPKADILKSDIQDLLDSGALIPEVNMSQLT
                     SKPTPAKDFKHVTEEVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTHGNIYAPVA
                     GLVTSVFPTKHAFGLLTDNGLEVLVHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIQSAERETTIVVAFTNTTEIQDVTLTSLGNQPAKTKVATVEL"
     gene            complement(5443..6192)
                     /locus_tag="VH752_001374"
     CDS             complement(5443..6192)
                     /locus_tag="VH752_001374"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEQ8289719.1"
                     /translation="MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLTDEAEVVLVFDDG
                     VKYLAKVTNSMAHELEIIEILPDQVELPVKVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEHKAEFLKSLSS
                     FDHIFIAYEETAKAGQLATLAREVKEVKPGAKILFIFGPEGGISPAEITQFEAAGAIK
                     VGLGPRIMRTETAPLYALSALSYALELQASK"
     gene            complement(6192..7145)
                     /locus_tag="VH752_001375"
     CDS             complement(6192..7145)
                     /locus_tag="VH752_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEQ8289720.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYIGQKD
                     RFGELYPDVEQSDMIAITAYYPSSTNLADVIATINEQLAELASFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQENIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLVKKEGYLILSGIISEK
                     LDMVLETAFSAGFFLETHMVQGEWNALVFKKTDDISGVIGG"
     gene            complement(7216..7686)
                     /locus_tag="VH752_001376"
     CDS             complement(7216..7686)
                     /locus_tag="VH752_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEQ8289721.1"
                     /translation="MAKYGFLSVLEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEVTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKADFFLEWNAQAFEEGQQGLEEAASYPYPRY"
     gene            complement(7751..7972)
                     /locus_tag="VH752_001377"
     CDS             complement(7751..7972)
                     /locus_tag="VH752_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEQ8289722.1"
                     /translation="MFDYIEGLIERRHSFIKEHFVASIDNFLNFREYRMLEGKGSISM
                     KFAKEKASREYVEFNKLQKINPDKGHKKI"
     gene            8162..8278
                     /locus_tag="VH752_001378"
                     /pseudo
     CDS             8162..8278
                     /locus_tag="VH752_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            8380..9243
                     /locus_tag="VH752_001379"
     CDS             8380..9243
                     /locus_tag="VH752_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISSpy2 family transposase"
                     /protein_id="HEQ8289723.1"
                     /translation="MSHLQYTAKSHHLQWNVTQLSTICHQLYRDYCPEGLKHRRNISL
                     SKVSDESLLVLPVLQAELGIKSQRHFYRICQLFPCGHLLERSRFNRRARQLIWLVQVI
                     RQAMNARISPDTIVIIDSFPLPLCQPVRNHRAHIFKGVADIGYNTSKHLWFYGFKVHM
                     LVTLSGYILNYVVTPASVHDIKAVHELLEECDQSIILADLGYLSRELKNNLEQKGYQL
                     WTPLRQNMTGAKQHNHWRVMAMRRTIETRFSELCSLFDIEHTLTRGVTGLQLRIEQII
                     LAYNLKYVEFN"
BASE COUNT         2671 a         2230 c         1898 g         2956 t
ORIGIN      
        1 gttggtgtca cttctgggct cttttctgga gctttttcgc ctggttggcc tgcttcgcct
       61 tctgggctct tttctggagc tttttcacct ggtttgcctg cttcaccttg tgggccttgc
      121 tcacctggtt tgcctgcttc accttgtggg ccttgtgcgc ccgtttcacc tttttcacct
      181 tgtgggccta ctggaccttg agctccagtt tcaccgcggt cacctttttc acccttttcg
      241 cctggagctc cagcttcacc ttgtgggcct tgagctccgg tgtcaccttt ttcaccttgt
      301 gggccttgga ttccagcttc acctttttca ccgcggtcgc ctttttctcc agctttacct
      361 ggcttacctt ctggaccggc tgggccagca ggaccagtct cacctttagg accggctggg
      421 ccttgtgttg cacctttttc aattaattct acaatttttt taagtgcttc ttgagtatac
      481 tcctctaatc ctttttgata tttatcgtaa tcaagatatt ttctgccaaa aactccataa
      541 ctgtggtatt tttcttcagt aagttttgga tatttagcct ggatttcctg tttaattttt
      601 tcaatttctt gttgtctctg ctccgatgtc agaggagcag ctgaaccctc agccttaacg
      661 ctgcttgccc cgcctccaaa ggctagaagg acggcagcgg ctgaggttag cccgtagcgc
      721 cagaccagtt tgttgagatt atggtgtttt gatgtcaaca tatgttgttc tctctttctc
      781 tattagatga tgtgttttag ataaaatatc atggtagcta gacctgatta tttataatag
      841 tattataata gagtgtgaaa acgattgtaa agttctcttt tgttgttcat cttttagatt
      901 ttgactgatt gctcttttgt ggagatcaga gggccacttt ggcacttgcc tgcctcgttt
      961 ttaagtcgag agcatccgga tcctttcttt aaggtcactc ttttttgaaa tgttactttt
     1021 tttgactgct tgtaccttct tgcctggttg aataccgcta ggtttcttct ttacttgttt
     1081 gttggtcttg attgtggtaa gcttatgaag aaaccttgta ggataaaaaa aaggagacac
     1141 ttatgcctca gattaccctt gtttttatta gcctcagtgg caacaccctt agctttgtca
     1201 agcgattatc cctttattta gcagacaatt acgattacca tgtcaaacag attaacatta
     1261 aagatttgaa gcacgagact tttcccgtca aagaagagtt tgtggcgatt ctacccactt
     1321 atctagaagg cggaaatggc gttgactctg gtgaagtgga gatcctcacc actcctttgg
     1381 gagagtttat tgctgcccat ggcaacgccc agcattgtct tgggattatt ggttcaggca
     1441 acaaaaactt taaccatcag tattgcctaa cagccaaaca gtatgccaaa cgctttggct
     1501 ttccactgct aggtgatttt gagctacgag ggactcctga tgacatttcc cggttagccc
     1561 aggtcatcat ggaggccagc tccagacact cgtctaacag ctagacagtc tatagatgat
     1621 catagctgtt ttaaaaaaga gcgcttttag gacaccaatt agggaagtcc cctaccctac
     1681 gccttgtgat ttcttagcct ttgaaaagag cgatcacagt gcttcttggg gcttgggatc
     1741 acaccaagca ctcctagaaa tacccccatg cagactttag caaggcaact gaacgcttgg
     1801 tggtactttc tgtgaagctc agatggttgg tgacaaaaaa agccctgttt ttctagggtt
     1861 actgacatga ctaaagaggt ctaaccagct ctatcttgag ctggttagac ctctttgtgt
     1921 tctcctaatc cccctagacg atcgcagttt cttatgtgtc ttggcttctc ctatgtggtg
     1981 tgcctgttag cacatgctct aacgccagtc taatgtcact tctagaccat agtggtcact
     2041 gacgactggc gcatccccgc cttcaaaggt aatcttagaa gaccggatga tgaagtcctt
     2101 actggtaaag acatggtcca ccttgagggc ttctttgtta ccttgccagc catcaatatc
     2161 agccacgatg ctatgatcac caaacacatg gtcagcaacc ttatggctat cttgtaggtt
     2221 tagggggcta cccatcatca cttgataacc gtcttgatcc gttggattgt taaaatctcc
     2281 cataagtagc aagggacagt ttagtgtgag cagctctttt tctaatttct cccactcccc
     2341 taaaaacccc ttgtcaaacc atgacaagtg cacattgaca acagcgactt ctttgccatc
     2401 taacgtggtt ttggctatga gtgagcgtct ggtgtggtag tctgtttcat cagccatcgc
     2461 tgacacgaga atatcggaaa catgaatagg ttgttttgat aagattgcca cgccttcctg
     2521 gtaaatgtca tagccaatat gattataagc ccaagaccaa taatagtgct gccctcgttt
     2581 ttgtaaataa tggatcaaga gcagggcaaa atgatcttta tgaatgcttg gtgtccctga
     2641 aagagcttga tatcttggta aatctgtggc cagctcgctc tcgattaact gattgatttc
     2701 ttgcaagcaa atgatatcgt atttttcagc taaaatatgc tcagccaaag caactagttt
     2761 tttaagagtg ttggcttgca tccacgaatg ggtattcagt gttaatacct tggtcattaa
     2821 tcctccttta taagacaaga agagccatag ggcctaaacc agctatggct attacctatc
     2881 actatctcgt gatgttttgt taaagttcta cggtggctac tttagtctta gcaggttgat
     2941 tgcctaaaga agtcaaggtc acatcttgga tctctgttgt gttggtaaaa gcaacaacaa
     3001 tggttgtttc acgctctgct gattggatgg cagcaagatc tgctacgact aataggtcac
     3061 ctgcatgaac ccgttgccct tctgagactt tgactgaaaa aggcacacca tttaaggcga
     3121 cggtatcaag tcccacgtgc accagcactt caagaccatt atctgtcagt aaaccaaagg
     3181 catgcttggt cggaaagaca ctggtcacaa ggccagctac gggtgcatag atattaccat
     3241 gggttggttc aaccgcaaac ccatctccca tcatcttagc agcaaacacc tggtctttca
     3301 cacctgtgat tgggagaacc atcccgtctg ccactgatag cacttcttct gtcacgtgtt
     3361 taaaatcttt tgcgggagtc ggtttgctag tgagctgtga catattgact tctggaataa
     3421 gagcacctga gtccagcaaa tcttgaatat ctgatttcaa aatatctgct ttagggccat
     3481 agaccgcttg aacgccgttg cctttttgga tcaagcccat agcgccagct tttttccaat
     3541 cgtcctcagc gccaacctta gcggggtctt tgaccgtcac tcgtaagcgc gtcatacaag
     3601 catcaacatc atcaatattg cctgacccac caaggagatt aatgatttgt acgacttgag
     3661 agttgctatc tgctacttgg gtcggtgttg cttgagtgtt acggtcaccc agtatatcgg
     3721 catcgtagtt acctaaacgg ccagctgttg cgagatgcat tttcttgatc atcatatccg
     3781 caataaagta catgataact gcaaagagga tagaaaccca aacaaagtta atcacatcca
     3841 tgcctaggcc tgctttaagg gccatagggg tacgggtcaa taattcaatg ttcccaaatg
     3901 agtgaacacg aaggttcaca aggtcagcca tggcaaatga agctccttga acaagggcat
     3961 agacgatgta aagtggcatt gctgcaaaca taaataggta ctcaagtggc tcagttaccc
     4021 ctgtcaaaaa gaccgctgcc gctgctgaga tgaacatcat tttgtatgtg tgttttttat
     4081 cggcatctac attacggtac atggctaagg caacccccat caatgttccg gtagctccaa
     4141 tcatttgtcc taccttaaag cgagctggag tcacactatc cattaagtga ctataggctg
     4201 aagcgtctga tcctttaagg tgaacaaggt cagttaccca agcaagccac aaagggtctt
     4261 gaccaaatac ttttgtcccc gctgctgcac ctgtcatgac ctcataagtt ccaccaagag
     4321 ctgtatagtt cattgggatc gtcaacatgt ggtgaagacc aaatggcaac aagagacgtt
     4381 ccaaggtacc atataggaat ggggctagga ttggagctga atcttgtgaa gaggcaatcc
     4441 acatcccaaa actgttaatc ccagactgga tcaccggcca aacaacaacc aaaataaggg
     4501 ctacaaaaat agaacgtaaa ataacaacaa atggtacaaa acgcttccca ttaaagaagg
     4561 tcaaaatttc aggaagttta cgataattat agtatttgtt ataggccgtt gctcctacga
     4621 accctgcaat aatcccaaca aaaacccctg tgtttaaagc tggagattcc aagacactgg
     4681 tgaaataatc tttaacgatc atttgagtgc caaggaggct tgtgattttt gcttctggat
     4741 cagctagcat agcactagac acgccataaa aagcacctgt tatccgattg atcaagacaa
     4801 aagcaagacc tgaggcaaag gcccctccag ctctttcttt agcccaacta ccaccaatgg
     4861 ccaaggcaaa tagcaagtga aggttaacaa taacagccca accaatttga gcgataatgt
     4921 tgcctagaga tgccaaaaat gctgagtcgt ggttaatcat tggaatagag tttccgatac
     4981 tgatcattaa accagccgct ggcataacag caataacgac cataagacat ttcccaaatt
     5041 tttgccaaaa ttcgaaacga aacagttgtt tgaatgatgt tttcattcta tctctccttt
     5101 attatcccct tttagccata gctgccctta aggcgctttt gtgacacaat gatatagact
     5161 acaactcagc gatgcaatcg cttgcattga tctgatacct ttattatact agcctcgcag
     5221 caatttgtaa agcgttttct tagacagatg ttagtcgttt ttcttcatta aatggtagtg
     5281 taacttacca aaaagaggac aagagcccat ttcctttttt ggtgatgagc ccatcaacgc
     5341 tcttcaaaaa aacaagaagc aataaaaaaa ggccgttgac acaatcgtga actgcctgtt
     5401 cgtttcttta gcctcttctt tctaaagtaa gcagcagctt acttattttg atgcctgcaa
     5461 ttccaaagca taactaagag cacttaaggc atatagcggc gctgtctctg ttcgcataat
     5521 gcgagggcct aagcccacct tgatggcacc tgctgcctca aactgagtga tttcagctgg
     5581 ggaaattccc ccttctggtc caaagataaa gaggattttt gcccctggct taacttcttt
     5641 tacttcacgc gccaaggtcg ctagctgacc tgccttagct gtttcctcat aagcaatgaa
     5701 gatatgatca aagctagaaa ggctttttag aaactctgct ttatgttcaa aaaggtgaac
     5761 ttcaggtacc ctatttcgtt tgctctgctc agccgctcct aatacaatct tagccaattt
     5821 gtcttctttt ttagctagtt ttttgccatc ccatttgact actgaccagt ctgcggggta
     5881 accccatagc gcactggctc ccaattcagt cactttctgg gcaatcgtgt ctaacttatc
     5941 gcctttagga aatccagaag caatagtcac tttaacaggc aattccacct gatctggcaa
     6001 gatctctata atctctaact catgagccat actattggtc accttggcta aatacttcac
     6061 cccatcatca aagaccaaga caacttcagc ctcgtcagtc aagcgcatca cttgaaacat
     6121 gtgcttgatg gtatctttat cggtaatcgt tacctttttt tctgctttgc ctttaataaa
     6181 atactgttgc attagccgcc aatcactcct gagatatcat ctgttttttt aaagacaagg
     6241 gcattccact caccttgaac catatgcgtc tcaaggaaga atcctgcgct aaaagcagtt
     6301 tccagaacca tatccaattt ctcagaaatg atgcctgata agataaggta accttccttt
     6361 ttgaccaaac ggtaagcatc atcagtcaat aagactaaga tatccgccaa gatattggct
     6421 acgatcacat ccgcttcttg gctaacccct tttaaaaggt cgccagcagc cacatgaatg
     6481 ttgtctgttc cttgattcaa atcaatgttt tcttgagcca ctcgaacagc cacgtcatct
     6541 aagtcataag cgtagatggt tttagcacct agtaaggaac tggcaatcga gaggacacct
     6601 gatccggtcc ctacatcgat aactgtctca ccaccacgaa ggacttgttc caaggcaaaa
     6661 aggctcatct tagttgtggg atgtgtccct gtcccaaatg ccattccagg atctaattta
     6721 atcacttttt ccccagcaga agcgtcataa tctgtccaac tagggacaat ggttaaatcg
     6781 tgcgtgatac gagctggttc atagtatttt ttccaattgt ctgcccagtc ttcctcagcc
     6841 aattcttgac tatccacagt aacctgacca acctgtaacc caaaactagc taactccgct
     6901 aactgttcat taatggtcgc aataacatcc gccaaattag tgctacttgg gtaataggct
     6961 gtgatagcta tcatgtcaga ctgctccaca tctgggtaca actccccaaa gcggtccttt
     7021 tgcccaatat agtctgcgct atccgcgata gcaacccctt gactgcctgt ctcaatcaac
     7081 acatgtgaaa cagcctcttg cgcatcacga tgcacatgaa ccgtcacttc ttgccatgtt
     7141 tccatagtta tgtcctttct attttgacct tttggttatt gtcctaatag tgttagcacc
     7201 atctcgtgtg tcaccttagt accttgggta agggtaactt gctgcttctt ctagtccttg
     7261 ttgaccttct tcaaaagctt gcgcgttcca ctccaagaag aaatcagcct tgctgtcatc
     7321 tgctaagaaa tccatcaaat cgctgtgccc ttcaatagca acatcgttga gaaattgcgc
     7381 aaagtaagct aagaattcac gagaaaagcc ctttttagcg tcaaaaggaa tggtaaccaa
     7441 ataatctgct gcatcaaatt gagacttggc agggttgtaa aagagcacat agtcctcaaa
     7501 aacaatatca tcttgggtca cttcgccact atcatcaatg gtttttatgg cctctttatt
     7561 ttgagcttct aacacaaaag tcacttcaac cgcatgattt ttcttgtccc aatccatagc
     7621 ataatcatat tgaaaatgct tgtccatttc ttcttctaat actgataaaa agccgtattt
     7681 tgccatcttt tttataccta atctttctaa ctgttgctgt ataaggcttt gtaagacctc
     7741 attagtttac ttaaattttt ttgtgtccct tatcagggtt aattttttgt aacttattaa
     7801 attctacata ctcacgacta gctttttctt ttgcgaattt catagaaata gagcctttac
     7861 cttctagcat tctatattca cggaaattaa gaaagttatc tatacttgca acaaaatgct
     7921 ccttaataaa tgaatgacgt ctctctatca aaccttcaat gtaatcaaag atggattatt
     7981 gcctttgaca atatgatcaa cactaatatt atagcacaaa ggagctaacg atgggtgctg
     8041 aagaacaact gaaagaattc catacgccaa tagccttgat ttgatgtata acgagcttta
     8101 ccagtttgat atagaatatc attcctttag acgtaaactc cttgaaataa gccgagaatg
     8161 cgccctagag gaattagacg ccatcaagac aagagactac gttcattggt ggaactatca
     8221 tcgcattcac agtagcctca gttaccaaac acatatgacc ttgagaaaca tcgcctaact
     8281 aaatatactt tactcgtggt gctagttaat gtcagaatac aataaaaagc ccaaaaggac
     8341 tatactgtaa gtgttcaaac aaacaggagg ttagccccaa tgagccactt acagtatacc
     8401 gctaaatccc atcacttaca atggaatgtc acacaattat ctacgatttg ccatcagcta
     8461 tatcgtgatt attgtccaga aggcttaaaa caccgtcgta acattagctt gtccaaggtc
     8521 tcagatgagt ctctactagt cttgcctgtc ttgcaagctg aattaggtat taagtcacaa
     8581 cggcattttt atcgtatctg tcaactcttt ccttgcggtc atctccttga aagaagtcgt
     8641 tttaatcgac gagcaagaca attaatttgg ttagtccaag tcattagaca agccatgaat
     8701 gctcgaattt ctcctgatac aatagtcatc atcgacagtt ttcctctacc actttgtcaa
     8761 cctgtccgta atcatagagc ccatattttt aaaggagtgg ctgatattgg ttataacacc
     8821 tctaagcacc tgtggttcta tgggttcaaa gtccacatgt tagtgacttt atcaggctat
     8881 attcttaact atgtggtcac accagcttct gttcatgata ttaaagcggt tcacgagtta
     8941 ttagaagaat gtgaccagtc catcatttta gcagacttgg gctaccttag ccgtgagctg
     9001 aaaaataatc tggaacaaaa aggctatcag ctatggacac ccttgcgtca aaatatgaca
     9061 ggggctaaac agcataatca ttggagagta atggctatga gacgaactat tgaaactcga
     9121 ttttcagaac tttgttccct ttttgatata gaacacacac tgactagagg agttacagga
     9181 ttacaattga gaatcgaaca aattatcctt gcttataatc tgaaatacgt tgagtttaac
     9241 tagcaccacg agtatacttt ataaatgttg cacagaaaac tgttgccttt tcaaagcttg
     9301 gctggagttt gtggaacatt acaggggtgg aggactaaag caacaaaaaa agaagcaaga
     9361 gatgaattgc ttcttttaca gatagattag tcgttgtctt ggcgttcttg taagttcctt
     9421 tccaaactat tgatgacatc atcaacttgt cctaaactta gagcttgacg agcttgccta
     9481 tagtagcagt taaaatagcc tttatattcc tcaccaacga ttccatactg atctagtagg
     9541 tttctaattt ttagctcgga agcatatcta gcttctctaa gaacttgagt gccgttttat
     9601 cttagaaagc ggctagtatt atagtaagga ttatttaagt atgaagatgc acttacagaa
     9661 ttagttgata gagcaacggt gctagctacc attaatgcag cggcacttag taatagtgtt
     9721 tttttcataa aaaacctctt cttttaattt tatgt
//
LOCUS       DAUGHY010000048         9675 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.048, whole genome shotgun sequence.
ACCESSION   DAUGHY010000048 DAUGHY010000000
VERSION     DAUGHY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9675
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.048"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            32..238
                     /locus_tag="VH752_001380"
     CDS             32..238
                     /locus_tag="VH752_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ8289724.1"
                     /translation="MQENAFEQLIDDSGIKKKVIATKMGFTRSGFYQKRKKPKKSFDA
                     SEVAMLADILGVDPGKVLEAILIS"
     gene            complement(484..693)
                     /locus_tag="VH752_001381"
     CDS             complement(484..693)
                     /locus_tag="VH752_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289725.1"
                     /translation="MNLEELTPLLNSIDDFETVILHSLVGDFVIDHWIEPNRKNETLI
                     FMHNDQTTELKLSAILGTSTIPKSL"
     gene            complement(856..1086)
                     /locus_tag="VH752_001382"
     CDS             complement(856..1086)
                     /locus_tag="VH752_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289726.1"
                     /translation="MEIFSYPENAKELYEKHKKAIEQACEPIATMLALNNPELTDLAN
                     EALELYARTIYNACVDASIIIRNREYQHYRDQ"
     gene            1231..1416
                     /locus_tag="VH752_001383"
     CDS             1231..1416
                     /locus_tag="VH752_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8289727.1"
                     /translation="MWGKLSKILSERGMTMYQLAKLSGVNKSHFSDLKSGKIKNLSWP
                     NMVKISDALGVSLDEFK"
     gene            1489..1734
                     /locus_tag="VH752_001384"
     CDS             1489..1734
                     /locus_tag="VH752_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289728.1"
                     /translation="MEIVHIKDIVTEPYSADDETLGKIFGYTKRQMQDRRYEMVKIPY
                     FSKYLLEQGGRVTIDGMREYLFYRKSIEWEKDKEKYL"
     gene            1731..1871
                     /locus_tag="VH752_001385"
     CDS             1731..1871
                     /locus_tag="VH752_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289729.1"
                     /translation="MKLLDFIFAKPKKQEEKPKWTIENNGWEANTRRYNQKHGLPAKQ
                     IW"
     gene            1881..2093
                     /locus_tag="VH752_001386"
     CDS             1881..2093
                     /locus_tag="VH752_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289730.1"
                     /translation="MDQYLINDNEFLRDENTRLNKEFSELNHAVAKANLLDVIIEDGY
                     ILQSTLDKCTAKLDEIDQKELKTWKN"
     gene            2081..2263
                     /locus_tag="VH752_001387"
     CDS             2081..2263
                     /locus_tag="VH752_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289731.1"
                     /translation="MEKLTLKKEKENTATLPFFVGIDLHAQIKEISTETGIPMRRIIE
                     RMIRFSLDNLEIMEGD"
     gene            2373..2684
                     /locus_tag="VH752_001388"
     CDS             2373..2684
                     /locus_tag="VH752_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289732.1"
                     /translation="MGSEGLRLRIYINKHKKMIIAPDYNDRYGGISNTTIQLRTGKLT
                     EAIEQEVGEAILDIIKKYEPIFDTPIIDDLFREKEQAIRKMVDYDTALTEMVEVEDEN
                     Y"
     gene            2671..3363
                     /locus_tag="VH752_001389"
     CDS             2671..3363
                     /locus_tag="VH752_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ8289733.1"
                     /translation="MKITKATEITKTHNWRILIYGKPGNGKTYLTNYLQGKTLILDMD
                     HSSKTIAGNENIDIIQFDRTHPSDFMTEFLIELPELIKEYDNLVIDNITSWQSDWFIE
                     QGRKSKNGITNELQQYNMWTNYYLRVLTTIYSQPINIFVTAWESTQDLTLESGQIITQ
                     YIPDIRKQVLSQTLGLTDIVGRVQVNPKTGGHGILLQGSDGLYAKNRLDNRTVCKAEE
                     LFNFGDTVNETT"
     gene            3459..4694
                     /locus_tag="VH752_001390"
     CDS             3459..4694
                     /locus_tag="VH752_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ8289734.1"
                     /translation="MVMAHIAKSATDKGNRVLFFSHRKEINEQVEKTFAVNGVNSNLL
                     TIGGVQSLVRKLDSLFQPEVILIDEAHHSKAKSYLKIIDYFKNAYVLMFTGTPVRLNG
                     DGFDDIADDLVVGKSVKWLQEHGNIANFKYYAPSMIDNSVLKKRGGEFTKDSVDQSMK
                     SVIYGDVIKHYEKLAKGKQAIVYTHSVEASHLVSDTFNQAGYQSQAVSGKTPKNEREE
                     AMRAFRDGDLKIMVNCELFTEGIDLPNVDVCIMLRPTQSLSLYLQFAMRPLNPRDGKT
                     AIIIDHVGNVERFGLPNMDREWRLDGETKQKQSAKIGEPTTRVCDNCYATYWSDTRIC
                     PECGHENELTKREIEEIKEAELQEISEQKQLKLKNRVSTYQTPDMCQTMDELTEYRKQ
                     HGYKPGWQYYIAKKLGILY"
     gene            4710..5168
                     /locus_tag="VH752_001391"
     CDS             4710..5168
                     /locus_tag="VH752_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEQ8289735.1"
                     /translation="MFTIDYSKAKEFASITDGTYETFIEKAVQDATKNGADFINIHFR
                     IRKDFQQEFQNNIIFHRIFAKKEDGKYPEGVIHNLAKQAGIPEGTQFKSLDDYLNQLL
                     NKCLKITVKNETSEYNGNTYNNLNVKRIEKSDIPSMVNPVEEFKEDDLPF"
     gene            5171..5983
                     /locus_tag="VH752_001392"
     CDS             5171..5983
                     /locus_tag="VH752_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEQ8289736.1"
                     /translation="MRGMIDYAIYYQQKGFSVIPISKDGKKPLVAFADKPAFTEHELR
                     LLWKDNPDANIALRTDSFFVIDIDVHNGVDGLKNLREWEHARLIPKTLQATTPSGGRH
                     IYLKKPQGVSMAQNIGFVDGVDLKAHVNNYVLVPPSNNAKGMYVWDKVHSPTSGEMTE
                     APLELITVLQELKPAYEYDASSFTSGDYQGSNKTAKLFETIVLGFGDTGGRNNSLAEF
                     VGGLLLRNVDVEIAYTLAKMANHKTVDPLNDKEFDRTFKSMCDKELRRRSGL"
     gene            5973..7448
                     /locus_tag="VH752_001393"
     CDS             5973..7448
                     /locus_tag="VH752_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEQ8289737.1"
                     /translation="MDFEFYREKLNEEPGIEPGKPTTWSAIKSKLIAYRRDWLEEAGK
                     DVKNLSELAVAIGINKYLHVITLENGKVAIYDPDQGYYIKDYKFAYKLIHILQPTFNE
                     TKCRNVLFMLASMDRKYGAMDFEPEFQDVRRYVLVKNGIYDKYKRKLLPFDYRFINFS
                     TIETELIPNAPLPTIDGWDVESWLLDLMSGDKDLVKLLWQVVAASLNGNYSYRKSIWF
                     VGNGNDGKGTFQQMISNLVGFKNVAPLKLNQFSERFGLAIIEGKTVIIGDDVQAGIYV
                     DESSNFNSVVTGEPVSIEKKGENPYMAIFKKTVIQSTNGMPSFKNKSNGTYRRIIIIP
                     FKKTFSSKEDNWAIKDDYINRKEVLEYVLWKAINIDFDRFSEPKATQERMHAFKRDNN
                     TILAFIDDWFERFTSTVLPTRFLWWLYKEWCKDNGHTPLKQSTFENELSTNIPDGWVK
                     KKSRVAGKFFPSDEIPTEYGHFPWNNEKDANGSMQSYVKII"
     gene            7710..8123
                     /locus_tag="VH752_001394"
     CDS             7710..8123
                     /locus_tag="VH752_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289738.1"
                     /translation="MYDILKDYEDLPIPSESIYYHDWLIGNITSEEAKEHFYRSDHPK
                     GFLELSEDKQEKLLHWCKQLEKTKTYENGHTSYGLKHKFEYRKNGFYVTNGQFKGAML
                     LAGFKPKDKNKLNWVFAFSVKSLRKIIDTKRYVMV"
     gene            8120..8440
                     /locus_tag="VH752_001395"
     CDS             8120..8440
                     /locus_tag="VH752_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEQ8289739.1"
                     /translation="MTTESLIQNQIRVGLSQAGHMVFRANVGKVKTADGRFFDTGLPK
                     GFCDLFGFKPDGQVFFIEVKNETGRVRPEQKKFMEVMASRGALVGVARSVEDALKIVN
                     DTSR"
     gene            8424..8780
                     /locus_tag="VH752_001396"
     CDS             8424..8780
                     /locus_tag="VH752_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289740.1"
                     /translation="MTLVDDFYKQMEPSIKAFLDDNIIITDPEEADRVYKSVKYYKNL
                     NRLPPPNVLDWFKQIYTTEEMITLIKQSYRLKQKKTDEDDEIYEKWMFKNYGDVKLVK
                     KVKRMDALAKAWEMGL"
     gene            8905..9186
                     /locus_tag="VH752_001397"
     CDS             8905..9186
                     /locus_tag="VH752_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HEQ8289741.1"
                     /translation="MTYKINPESMQVAFDKNYQTFLDKNADYGNSFEKSLDDLGIVAG
                     VVRIGDKYNRVCNLIKNKQNVSESLSDTLNDMANYCMMLAVWLEGQKDD"
     gene            9179..9364
                     /locus_tag="VH752_001398"
     CDS             9179..9364
                     /locus_tag="VH752_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289742.1"
                     /translation="MTKKHVVRVYNKGITATYMVYDKNLFKEHEFATKREAMQFIRKL
                     ELANEKRSTEYFMREAE"
     gene            9361..9630
                     /locus_tag="VH752_001399"
     CDS             9361..9630
                     /locus_tag="VH752_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289743.1"
                     /translation="MKKPNRYPYSKSKFNGCIYQLHAASFKDEQYVEDLKSCGIHYQI
                     TKIGYFPDIFIKIDNLEQLQILIDKTGHDLILSKDQIWIYDDYME"
BASE COUNT         3549 a         1555 c         1962 g         2609 t
ORIGIN      
        1 gataattcca aattagaaag ggggtggggg aatgcaggaa aatgctttcg agcaattgat
       61 tgatgatagc ggaattaaaa agaaagttat cgcgactaaa atgggtttta cacgctctgg
      121 attttatcag aaacgtaaaa aaccaaaaaa atcatttgat gcgtcagaag tggcaatgct
      181 agctgatatt ttaggggtag accccggaaa agtcctagaa gccattttaa tttcatagaa
      241 aagttgacaa aaataacaca aaatttagaa aggacacata tgaacgaaat agaaaacaaa
      301 ctagaagaac ttgaagaaat ggtaataaac atggatgaag tagatgtagt gattccatgg
      361 aaggtagcaa aaaacctgct acaaagagca ggttacttat cagaagagga acatcgccta
      421 ctaagttgga gactcggaaa atcaaaatat gcaggcaaac gttccgaaaa agtccgcaat
      481 ttgctagagg gacttaggaa tagtggaagt tccgagaata gctgataatt ttaattctgt
      541 tgtttggtca ttgtgcatga aaatgagagt ttcatttttg cgattaggct caatccaatg
      601 atcaataaca aagtctccga caagactatg taaaataaca gtttcaaaat catcaatact
      661 attcaataga ggagttaatt cttctaaatt catttagtta ccctccttcc taataggata
      721 actacattat acaacagaaa ggaagccaca tgagaccaaa acgatatccg tatagcagaa
      781 aagaaaaaag cacctatttc aaaatagatg ctagcatgat aaaagcagaa ttttttacca
      841 atcggcaatc agaacttatt ggtcgcgata atgttgatac tcgcgatttc gaatgataat
      901 agatgcgtca acgcatgcat tgtaaattgt ccttgcgtag agttctaaag cttcgtttgc
      961 taggtctgtt aattcaggat tatttaaagc taacatggta gcgattggtt cgcaagcttg
     1021 ttctattgct ttcttatgct tctcgtacag ttccttggca ttttctggat agctaaatat
     1081 ctccatactc cttccctcct ttccataatt tttgaataca acggtgagag gtcatattca
     1141 aataaattat atcagaatag aaaggaaaac acaacctata gttgtggata acttataaac
     1201 aactacatat tgtgttttgg tgagtgggta atgtggggta aattatcaaa aattttatca
     1261 gaaagaggta tgactatgta tcaattagcg aaactttctg gagttaataa aagtcatttt
     1321 agcgacttaa aaagcggaaa aataaaaaat ttgtcgtggc ctaacatggt caaaatttct
     1381 gacgctttag gtgtcagctt agacgaattt aaataacaaa aaaagcccga cgggaatcgg
     1441 actcaaaact agattaattt acttaattat aacatagaaa gggacagaat ggaaatagta
     1501 cacataaaag acatagtcac cgaaccttat agcgcagatg acgagacgtt aggtaaaatt
     1561 tttggatata ctaagcgtca aatgcaagac aggcgttatg agatggtaaa gattccatat
     1621 ttttcaaagt accttttgga acaaggtgga agagtaacta ttgacgggat gcgagaatat
     1681 ttgttttatc gcaaatcaat agaatgggaa aaagataagg agaagtatct gtgaaactat
     1741 tagattttat ttttgcgaaa ccaaaaaaac aggaagaaaa accaaaatgg acgattgaaa
     1801 acaacggttg ggaagccaat acacgtagat ataaccaaaa acacggtttg cctgctaaac
     1861 aaatttggta ggaggaaaga atggatcaat acctaatcaa tgataacgaa tttttaagag
     1921 acgaaaacac acgactcaac aaagagttta gcgagttaaa tcatgcggtt gctaaagcaa
     1981 atttattaga tgtcatcatc gaggatggat atatcctgca aagcacgctg gacaaatgta
     2041 ctgctaagct tgatgagatt gatcagaagg agttgaaaac atggaaaaac taactttaaa
     2101 aaaagaaaaa gaaaatactg caacattacc gttttttgta gggattgatt tacacgccca
     2161 aatcaaagaa atctcgacag aaacaggtat cccaatgaga agaattattg aaagaatgat
     2221 taggttttct cttgataacc tagaaatcat ggaaggggat taatgtatag ctttgaatat
     2281 tattgcgaaa attgcggcaa aaaatggtca aacatatcag catacaagga cttggaatgc
     2341 ccttgctgta aagaacccga tccgcaaatc acatgggaag cgagggctta cgattgagaa
     2401 tctacattaa caaacataaa aaaatgatta tcgcaccaga ttacaatgat agatacggtg
     2461 gtatatcaaa tacaactatc caattaagaa ctggaaaatt aacagaagcc atagaacaag
     2521 aagtcggcga agcgatttta gatattataa aaaaatacga acctattttt gacaccccaa
     2581 taattgatga tttatttaga gaaaaggaac aagcaatcag aaaaatggtt gattatgaca
     2641 ctgccttaac tgagatggtg gaggtggaag atgaaaatta ctaaagcgac agaaattaca
     2701 aaaactcata attggcgcat attaatctat ggaaaaccag gaaacgggaa aacttactta
     2761 actaattact tacaaggcaa gacactgatt ttagacatgg atcactcatc taaaacgatt
     2821 gccggaaatg aaaacattga tattattcaa tttgacagga cgcatccaag cgactttatg
     2881 actgaattct tgatagaatt accagaactt atcaaagaat atgacaattt agtcattgat
     2941 aacatcacaa gttggcaatc agactggttt attgagcagg gtcgtaaatc caaaaacgga
     3001 atcacaaacg aattgcaaca atacaacatg tggaccaatt actatttaag agtattgact
     3061 actatttaca gccaacctat taatattttt gtgacggctt gggaatcgac gcaagattta
     3121 acgcttgaga gcggacaaat tatcacgcaa tacatcccag acatccgtaa gcaagtatta
     3181 agtcaaacac ttggtttaac agatattgtc ggtcgtgtcc aagtaaatcc taaaacaggc
     3241 ggacatggaa ttctgctgca aggcagtgac ggactatatg caaaaaacag acttgataat
     3301 aggacagttt gtaaagcaga agaattattc aatttcggag acacagtaaa tgaaactacg
     3361 tgattaccaa gaagaattat taacagatat taggaggtca ttagcgactg gaaataaaag
     3421 aataatcgtc cagtcgcctc cctagaagcg ggaaaacggt ggtaatggcc catatcgcta
     3481 aaagcgctac ggataaagga aacagagtat tgttttttag ccatagaaaa gaaatcaatg
     3541 aacaggtaga aaagacattt gcagtaaatg gagttaactc aaacctgtta actatcggcg
     3601 gcgtgcaatc gctagttagg aaactagaca gcctctttca accagaagta attttgattg
     3661 acgaggctca tcacagtaag gccaagtctt acttaaaaat catcgactat tttaaaaatg
     3721 cttatgtatt aatgttcact gggacaccag tcaggttaaa tggagatgga tttgatgata
     3781 ttgctgatga tttggtagtt ggaaaatctg tcaaatggct gcaagaacac ggtaatatag
     3841 ctaactttaa atactacgca ccgtccatga ttgataattc tgtccttaaa aaaagaggtg
     3901 gagagtttac taaggattct gttgatcaat ccatgaaatc ggttatctat ggtgatgtta
     3961 ttaaacacta tgaaaagcta gcaaaaggaa agcaagctat cgtttatacg cacagcgtag
     4021 aagcctctca tttggtttct gacaccttta atcaagcagg gtaccaatca caagcagtga
     4081 gcggaaaaac gcctaagaac gaacgggaag aagctatgcg agcatttaga gatggtgatt
     4141 taaaaatcat ggttaactgt gaattgttta cagaagggat agacctgcca aatgttgatg
     4201 tttgcattat gttgagacca acacaatcgc tatcgctata tttgcaattt gcaatgcgac
     4261 cgttaaatcc aagagatggg aaaacagcca ttattattga tcacgttggc aatgtggagc
     4321 gttttggttt acctaacatg gatagagaat ggcgtttaga tggagagact aaacaaaagc
     4381 aatccgctaa gattggtgag cctaccacaa gagtttgcga taactgttat gccacctatt
     4441 ggtccgatac tcgtatctgt ccagaatgtg gacacgaaaa cgagttgacc aaacgtgaaa
     4501 tcgaagaaat caaagaagct gaattacaag aaatatctga acaaaaacaa ctaaaactaa
     4561 aaaatagagt tagtacctat cagacacctg acatgtgcca gacgatggac gaactaacag
     4621 aataccgaaa acaacatgga tacaagccag gttggcagta ttacatcgct aaaaaactag
     4681 gaattttata ttaaaaggag aaatacatta tgtttacaat cgattactca aaagcaaagg
     4741 aatttgcatc aattactgac ggaacttacg aaacatttat tgaaaaagcg gtccaagacg
     4801 caactaaaaa tggcgcagac tttatcaaca ttcacttcag aattcgcaaa gacttccaac
     4861 aagagtttca aaataacatt atcttccacc gtatttttgc caaaaaagaa gacgggaaat
     4921 atccagaagg ggtcattcat aatctcgcta agcaagcagg tattcctgag ggtacacagt
     4981 ttaagtccct ggatgattac ttaaatcaac tacttaacaa gtgtctcaaa attaccgtta
     5041 aaaatgaaac atccgaatat aacggaaata cttacaacaa cttaaatgtg aaacgtatcg
     5101 aaaagtccga tattccatct atggttaacc ctgtggaaga atttaaagaa gacgatcttc
     5161 cattctaatt atgagaggga tgatagatta cgcaatctat tatcaacaaa agggattttc
     5221 agttattccg atttcaaagg atggtaaaaa accattagtc gcttttgcgg ataaaccagc
     5281 ttttacggag catgagttgc ggcttttgtg gaaggataat ccagatgcca atattgctct
     5341 aagaacagat tcgttttttg tcattgatat tgatgtccac aatggcgttg acggtctgaa
     5401 aaaccttaga gaatgggagc atgcaaggtt gataccaaaa accttgcaag caaccacgcc
     5461 tagtggagga aggcatatct accttaaaaa accacaaggc gtttccatgg cgcaaaatat
     5521 cggttttgtc gatggtgtgg acttaaaagc tcatgttaac aattatgtgt tggtaccacc
     5581 atcaaacaat gccaaaggca tgtatgtgtg ggataaggtg cattcgccaa cctctggcga
     5641 aatgaccgaa gcgcctcttg agttgataac tgtattgcaa gaattaaagc ctgcttacga
     5701 gtatgatgct agtagtttta catctggaga ttaccaaggt agcaataaaa cagctaaatt
     5761 atttgagacg attgtccttg gttttggtga tacaggtggc agaaacaaca gtctcgctga
     5821 atttgtaggt ggtctattgc ttagaaatgt agatgttgaa atagcataca cattagctaa
     5881 aatggctaat cacaaaacag ttgacccttt aaatgataag gagtttgatc gtacttttaa
     5941 aagtatgtgt gacaaggaat tgaggaggag aagtggactt tgaattttac agagaaaaat
     6001 taaatgagga acctggcatt gaaccaggta aacccacaac atggtctgct atcaaatcca
     6061 aattgatagc gtatcgaaga gactggcttg aagaagctgg gaaagatgtt aaaaatttat
     6121 cagaactagc tgttgctatc ggaataaata agtacctgca tgttattacc ttagaaaacg
     6181 ggaaagtagc tatctacgat ccagaccaag gatattacat caaggactat aaattcgctt
     6241 ataaattaat ccatattcta caacctacgt ttaatgagac aaaatgccgt aacgtgttgt
     6301 ttatgttagc gagtatggat aggaaatatg gggcaatgga cttcgaacca gaattccaag
     6361 acgtgagacg gtatgtacta gttaaaaatg gtatttacga caaatacaaa cgcaaattat
     6421 tgccttttga ttaccgattt attaatttca gcactatcga aacagaactt attccaaacg
     6481 ctcctctccc aaccattgac ggttgggacg tggagtcatg gttattagat ttaatgagcg
     6541 gagataagga cctcgtcaag ttattatggc aagtcgtggc agcatcctta aacggtaact
     6601 actcttatcg taaatcaatc tggttcgtag gtaatggtaa cgatggtaaa gggacgtttc
     6661 aacagatgat cagcaatctg gttggtttta aaaatgtcgc tcctctaaaa ttaaatcaat
     6721 tttcagaacg cttcggtctt gcgattattg aggggaaaac ggtaattatc ggagatgatg
     6781 ttcaagctgg tatctatgtg gacgaaagta gtaactttaa ctctgtcgtg actggtgagc
     6841 ctgtgagcat cgagaaaaaa ggtgagaacc cttacatggc gatttttaaa aaaaccgtca
     6901 tacaatcaac aaacgggatg ccatcgttta aaaataaatc aaatggtact tatcgccgca
     6961 ttatcatcat cccgtttaaa aagacattct catcaaagga agataactgg gccattaaag
     7021 atgattacat taatcgtaaa gaagtgcttg aatacgtcct ttggaaagct atcaatatag
     7081 attttgatag gtttagtgag ccgaaagcga cacaagaaag aatgcatgct tttaagcgag
     7141 acaacaacac gatactcgct tttatagatg attggtttga gcgttttact tctactgtgc
     7201 tccctactag attcttatgg tggttgtata aagagtggtg caaagataat gggcacacac
     7261 cgcttaagca atcaactttt gaaaatgaat tgtcaaccaa tatccctgat ggctgggtta
     7321 agaaaaaatc tagagtcgca ggaaaattct tcccgtctga cgaaatacca acagaatatg
     7381 gccactttcc ttggaataat gaaaaagacg ctaatggaag tatgcaatct tatgtgaaga
     7441 ttatataaat gttccgtctt tgttccatcc ttgttccatg acatggaaca gcgaaaccct
     7501 ttggtattac tgggtttaat agacttctgt tccatgttcc atctttttct taatattaat
     7561 aaaaaaataa ataatataaa tatatagaga gaaaagcaaa aaatggcgga acatggaaca
     7621 gaaatggtct aatcccttga gccgcaagga attggtgcgt tccaccctgt tggaacattg
     7681 gtgggaacaa aatcaaaaaa ggagaaagta tgtacgatat tttaaaagat tacgaagacc
     7741 ttccgattcc ttctgaatcg atttactatc acgactggtt aattggaaat atcactagcg
     7801 aagaagctaa agagcatttt tatcgcagtg atcatcctaa aggtttttta gaactaagtg
     7861 aagacaaaca agaaaaattg ctgcactggt gtaagcagtt agaaaaaaca aaaacttatg
     7921 aaaatgggca tacctcttat ggattaaagc ataagtttga ataccgtaaa aatggttttt
     7981 atgttacaaa cggtcaattc aaaggagcta tgttgcttgc tggttttaaa cctaaagaca
     8041 aaaacaaatt gaattgggta tttgctttta gtgtaaaatc gctaagaaaa attatcgata
     8101 caaaaaggta tgtgatggta tgacaacaga atcattaatt caaaaccaaa ttcgtgttgg
     8161 attatcacaa gcgggccata tggtttttag agccaacgtt ggtaaagtta aaacagcaga
     8221 cggcagattt tttgatacag gtttaccaaa aggtttttgt gacttgtttg gatttaaacc
     8281 agacgggcaa gtgtttttta tcgaagttaa aaatgaaaca ggtcgagtaa gacctgaaca
     8341 gaaaaaattt atggaggtca tggcatctag gggagcgctt gtaggagtgg ctagatctgt
     8401 ggaagatgcc ttaaaaatag tcaatgacac tagtagatga tttttacaaa caaatggagc
     8461 cgtcaatcaa agcattttta gacgacaaca ttatcatcac ggatccagaa gaagctgaca
     8521 gagtgtataa atctgtcaaa tactataaaa accttaatag attgccacct cctaacgtat
     8581 tagattggtt taaacaaatc tacacgactg aggaaatgat aacgttaatc aagcagtctt
     8641 accgtcttaa acaaaaaaag acggacgagg atgacgaaat ctacgaaaag tggatgttta
     8701 aaaattatgg tgatgttaag cttgtcaaaa aagtcaaacg tatggatgca ttagctaaag
     8761 catgggagat gggactatga aaagacgcag gcagtttaat aaagatatta aatacacacc
     8821 taaatcttac gacagtgtgt tgccttatga tgtaaataag agcgagtgta aattgattat
     8881 agattttttg agagaggtaa aaggatgaca tataaaatta atccagaatc aatgcaagta
     8941 gcttttgata aaaactatca aacattttta gataaaaacg cagattacgg aaactcgttt
     9001 gaaaagtcgc ttgacgacct cgggattgtc gcaggagtgg tcagaatcgg cgataaatac
     9061 aatcgggtgt gtaatcttat caaaaataaa caaaacgtct cagagagcct atcagacacg
     9121 ttaaacgaca tggctaatta ttgcatgatg ttagcggttt ggttggaggg acaaaaagat
     9181 gactaaaaaa cacgttgtga gagtttacaa caaaggcatt acagcgactt acatggttta
     9241 tgacaaaaac ctgtttaagg agcatgagtt cgcaacaaaa agagaagcga tgcagtttat
     9301 cagaaaacta gagttagcaa atgagaagcg atcgacggaa tatttcatga gggaagcgga
     9361 atgaagaaac caaatcgtta tccatacagt aaatcaaaat ttaatggttg tatttaccag
     9421 ttgcatgcag ccagctttaa agatgaacaa tatgttgaag atttaaaatc atgcggcata
     9481 cattatcaaa ttacaaaaat tggttatttt cctgatattt ttataaaaat tgataatctc
     9541 gaacaattac aaatattaat agataaaaca ggacacgatt taatacttag taaagaccaa
     9601 atttggattt atgatgacta tatggaatga ggagtaataa tgataccaaa atttagagca
     9661 tttaataaaa agatc
//
LOCUS       DAUGHY010000049         9384 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.049, whole genome shotgun sequence.
ACCESSION   DAUGHY010000049 DAUGHY010000000
VERSION     DAUGHY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.049"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(162..1475)
                     /locus_tag="VH752_001400"
     CDS             complement(162..1475)
                     /locus_tag="VH752_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEQ8289744.1"
                     /translation="MTIIIMDSNSAHETDNLSVSFLNFCYNSLMKRHFLLLTFYLFLT
                     GLTAGLVAFILTKAIHLIQSLSFGFSQGSFSTMIASVPPQRRALSLLFAGLLAGLGWH
                     LLAKKGKDIQSIQQIIQDDISFSPWTQFWHGWLQLTTVSMGAPVGREGASREVAVTLT
                     SLWSQRCNLSKADQKLLLACASSAALGAVYNAPLATILFILEAILNRWSLKNIYAACL
                     TSYVAVETVALLQGRHEIQYLMPQQHWTLGTLIWPVLAGLILSLFAHAYKHLLEHLPK
                     ADAKSQWFIPKVLIAFSLIAGLSIFFPEILGNGKAGLLFFLHEEPHLSYISWLLVAKA
                     VAISLVFASGAKGGKIAPSMMLGGASGLLLAILSQYLIPLSLSNTLAIMVGATIFLGV
                     INKIPLAAPVFLVEITGQSLLMIIPLALANLIFYFSYQFYRFILK"
     gene            complement(1450..2409)
                     /gene="nrdF"
                     /locus_tag="VH752_001401"
     CDS             complement(1450..2409)
                     /gene="nrdF"
                     /locus_tag="VH752_001401"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ8289745.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINDIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
     gene            complement(2731..4890)
                     /gene="nrdE"
                     /locus_tag="VH752_001402"
     CDS             complement(2731..4890)
                     /gene="nrdE"
                     /locus_tag="VH752_001402"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ8289746.1"
                     /translation="MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYHPEFITELAGIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKANPINGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     ERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQAEDWKALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(4910..5128)
                     /locus_tag="VH752_001403"
     CDS             complement(4910..5128)
                     /locus_tag="VH752_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEQ8289747.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIEADNLVFSGFQPAKLKELI"
     gene            5521..5784
                     /locus_tag="VH752_001404"
     CDS             5521..5784
                     /locus_tag="VH752_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEQ8289748.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADAEDAIAAIEETMTKEGLA"
     gene            5789..7522
                     /gene="ptsP"
                     /locus_tag="VH752_001405"
     CDS             5789..7522
                     /gene="ptsP"
                     /locus_tag="VH752_001405"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEQ8289749.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARL
                     DVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGD
                     VIAVNGITGEVIIDPSEDQVLAFKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEV
                     LELSKEYVSED"
     gene            7707..9134
                     /locus_tag="VH752_001406"
     CDS             7707..9134
                     /locus_tag="VH752_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ8289750.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALIAGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVNFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLALAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEDDADITPLIDTSAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAVKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSVVFDIQ"
     gene            9230..>9384
                     /locus_tag="VH752_001407"
     CDS             9230..>9384
                     /locus_tag="VH752_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deacetylase"
                     /protein_id="HEQ8289751.1"
                     /translation="MKKLNVILVGLLSILMLSLAIVFINRWKLNEDSQRIVLAEKKKN
                     TSDLVIKA"
BASE COUNT         2981 a         1663 c         1970 g         2770 t
ORIGIN      
        1 ctcttcctca aggatgagtt ttacagcaga gagtttgaag gctttatcaa aggtttttct
       61 tggcataatg actattcctc gttttcactt aattttgtgt ctactttagt ataccaagtc
      121 caattcatcc gaagcttttt attaaaaaag taattaacta atcatttaag gatgaaacga
      181 taaaactgat agctaaaata aaaaatgagg ttagctaatg ctaaagggat aatcattagt
      241 aacgattggc ctgtaatttc aaccagaaaa actggtgccg ccaagggtat cttattgatc
      301 acacctaaaa agattgttgc accaaccata atggctaacg tatttgatag agacaaggga
      361 ataaggtact gactaaggat agctagtagt aagccactag cacctccaag catcatagaa
      421 ggagcaattt tacccccttt tgctccagaa gcaaaaacta aagagatagc gacagctttg
      481 gcaacaagta gccagctaat atagctcaga tgtggttctt catggagaaa aaagagtaat
      541 ccagctttgc catttcctaa aatctctggg aagaaaatgc tgagtcctgc gataaggcta
      601 aaagcgatga gaactttcgg aataaaccac tgacttttgg catcagcctt aggaaggtgt
      661 tccaaaagat gtttgtaagc gtgagcaaag agggaaagga taagaccagc caggacgggc
      721 caaataaggg ttcctagcgt ccaatgttgc tgaggcatta ggtattgaat ctcatgtcgg
      781 ccttgtaata aagcaactgt ttccacagcc acatagctcg ttaggcaggc agcgtatata
      841 tttttaaggg accaacggtt aagaatagct tccaagataa ataaaatagt ggctaaggga
      901 gcattgtaga cagcaccaag ggcagcgcta gaagcgcaag ctaataaaag tttttgatcg
      961 gcttttgaca agttgcatct ttggctccaa agagacgtca aggtaacagc gacttcccgt
     1021 gaagctcctt ctcggccgac aggagctccc atagatacgg tcgttaattg gagccaacca
     1081 tgccaaaact gtgtccaggg actaaatgag atgtcatctt gaatgatttg ctgaatagat
     1141 tgaatgtcct ttcctttttt agccaatagg tgccacccaa gacctgcaag aagtccagca
     1201 aagagaagtg aaagagctcg tctttgtggt ggaacgctag caatcatagt gctaaaagaa
     1261 ccttgactga atccaaaact gagggactga atcaggtgaa tggcttttgt taagataaaa
     1321 gcaacaaggc cagctgttaa acctgttaaa aagaggtaaa aggtcaagag taaaaagtgt
     1381 cgtttcataa gactattata acaaaagtta agaaaagaga ctgatagatt atcagtctcg
     1441 tgagcactat tagagtccat aattataatc gtcatcagac atagcttcta cagaaccaag
     1501 aagatagcca ttccctacct gtgagaaaaa gtcatggttt gatgttcctg ttgaaatacc
     1561 attcataaca attgggttaa catcgtttgc agtatctggg aataaaggat cttgtcctaa
     1621 attcataaga gctttgttag cattgtagcg taaaaaggtc ataacttcct cagtccaccc
     1681 tacgccatca taaagcgttt tggtgtattt ttcttcgttt tcatacagct gataaaggag
     1741 gtcatacatc caatcacgaa aattctcttg ctcatcttct ggtagttcgt taaaaccaag
     1801 ctggaatttg taaccgatat aagtaccatg tacagattca tcacgaataa ttagtttaat
     1861 gatttcagca acatttgcta gcttattatt tcccaaatag taaagagggg tgaaaaagcc
     1921 agaataaaaa aggaaagttt cgagatatgt tgaggcaact ttcttttgta aggcatctcc
     1981 attagcgtaa atgtcattga taatacgtgc tttttcttga agaaactcat tattattagt
     2041 ccactcaaag atctcttcaa tttctttttt ggtgtttaag gttgaaaaga tagacgaata
     2101 agatttagcg tgaacagatt ccatgaattg aatattgttt aagacagctt cttcgtgagg
     2161 agtgcgaaca tcagcacgaa tagcttcgac accagtttct gattgcatgg tatcaagtag
     2221 ggttaatcct ccaaaaacct tgccaacaag atctttttct tgaagagaaa gtttgcgcca
     2281 atcgtctaag tcatttgata aagggatacg ggtatcaagc caaaattgtt cggtcaactt
     2341 ttcccaagtt gacttgtcaa tgacgtcttc gatttcattc cagttaattg cttcataata
     2401 tgtagtcata gaaaaatgat ctcctaaaga gtagtaaagt gcgttaagga tatcgagata
     2461 aaccagtaga accgaccaaa gttctagtgg taataataag tttaaataga ttcaaaaggg
     2521 taacgaatga gttagtgagt ggtttaggca gatcgcctaa agcgactgcc gtagaagtaa
     2581 tagatgcttt agcatcatta gggcatatcg aggaattacg acacggagta aggaaagtcg
     2641 ataatacttt ccttacatag ttagtgagtg gtttaggcag atcgcctaaa gcgactgccg
     2701 tagaatttag catcattact tcttaaccaa ttaaatgaca caagattcac attggtttgc
     2761 gcccacttct tccccatcat ccgtaaaggt acggatatag tagatagatt taatgccttt
     2821 attgaaagcg tagtttcgta agatggataa atcacgagtg gtttgtttgc tttgtgtttt
     2881 ccactcataa agctccatag gcaactcact acgaaggaat agagttaatg acaagccttg
     2941 gtccacatgt tcggtcgcag cggcatagac atcaataact ttgcgcatgt ccatatcgta
     3001 agcagatgta tagtaaggaa tggtatccgt agacaaacca tttgcaggat agtagatttt
     3061 accaattttc ttttcttgac gctcttcgat gcgttgtgtg attgggtgaa tagaagcaga
     3121 gcagtcattg atataagaaa tagagccatt tggagcaact gctagacggt tttgatgata
     3181 aagaccatct ttttgaacgg cgtcgcgaag agctttccaa tcctctgctt gaggaataaa
     3241 gtgatcttta aagagacttt tgaccaaatc agattttgga acaaagtgtc ctgtaacgta
     3301 tttatcaaag taactaccat tagcgtactt agagttctca aagccaacaa aggtagtttg
     3361 gcgctcacga gcgatgttat tggattcgac caaggtccaa taattcagga gcataaagta
     3421 aatatcagta aactcgatgg attctggact gccatattca atatgatgtt gagcaaggta
     3481 agaatgtagt cccatagctc caaggccaaa agtatgagct tggctattgc catgtttaat
     3541 ggttggaaca gcttcaatgc ttgatgaatc agtaacaaaa gttagggcac gtgtcatggt
     3601 cttaatagaa cggccaaagt ctggtgaggt catcatgttc aggatattag tggaacctaa
     3661 gttacatgaa atatcagttc ccatttctac aaactcttgc gcatcattga taaggctagg
     3721 tgtttgaact tgtaaaattt cagaacacaa gttgctcatg atgatttttc cattgatagg
     3781 attagcttta ttagctgtat caatattgat gatataaggg taaccagatt cttgttgtaa
     3841 ttttgaaatc tctgtttcaa gatcacgagc tttaatttta gtcttagtaa ttttagggtt
     3901 tgccactaac tcatcgtaca tattggtaat gtcgagatag ttaaagggaa tgccatattc
     3961 tttttcaaca ttgtaaggac taaagagata catgtcctcg tttttacgag ctaattcgta
     4021 gaatttatca ggaacggtaa tccctagtga caaggtttta acacgtacct tttcatcggc
     4081 attttctttt ttagtagata agaaagcaat gatatcagga tgaaaaacat ttaggtaaac
     4141 aacaccagct ccttgacgtt gcccaagttg atttgaataa gaaaaactat cttcaaataa
     4201 tttcataaca ggaacaactc ctgaggctgc accagcatag cctttgattg gtgcgccagc
     4261 ttcacggagg ttagacaagg taatcccaac acctccacca atacgggata attgcaaagc
     4321 agagttgata gaacgtccga tagagttcat gtcatcagtt acttgaatca agaaacaaga
     4381 gaccaattca ccacgacggc ttcgaccagc atttaaaaag gaaggagtag ccggttggta
     4441 acgttggtta atcatttcaa cggctaaatc ttttgctaag tcttcttgac catctgcaaa
     4501 atacaaagca ttaaacaaga cgcggtcttc aaggttttct aaataatgct ctccatcatt
     4561 tgtttttaag gcgtattgct ggtagaactt gtaggctgcc ataaatgatt taaagcgaaa
     4621 attttctgat ttgattatgc cagctaattc agtaataaat tcagggtggt atttctgaat
     4681 aaaagctgat tcaatgtaat catttgagat taaatactca attttttccg taatggaagt
     4741 aaaagacatg gtgtttggca gcacattttc agcgaaaaaa gcttttaaag cttctttgtc
     4801 tttatgaagt ggaattttac cattaacagg acggttaatt tcattattta ggcgaaaata
     4861 tgaaatatcg ccaagatctt tgagactcat attatttttc cttgtcttat taaatcaatt
     4921 cttttaactt tgcaggttgg aagccagaaa atacgaggtt atctgcttcg ataacaggag
     4981 cagatgtgaa gccaagactt ttcacataat ccactttttc aggatgttca tcgatattaa
     5041 tttcctggaa gttggctccg tgttgttcta aaaatttctt agtcattttg cactgcatgc
     5101 aattattttt ggaatatact gtaatcatgt cttgtccttc taaaattagt aatggttcca
     5161 tcttaaatgg aattactata atagcatata aaaaataaag ctaaaaagca agctataaac
     5221 ttatctgaaa cacaacatat tgtgttgaaa taaaaaacag aaacctaaat attgtgtcaa
     5281 tctggattct tatggttaga gctctagcgt tttggtgata gaagctaagc agttttaata
     5341 aagcattaaa gtctgaaaaa taaattgtga aaaacgtgaa aataaaagaa aataatttca
     5401 cattttttgt cgtttaaata tttaatcttg aaaataaaaa agcattgtgc tataatcggt
     5461 tgttgtaagg gttatcatgt gatggctcaa aaatacacaa cttaaaagga gaaccatact
     5521 atggcttcaa aagactttca cattgttgca gaaacaggta ttcatgcgcg tccagcgact
     5581 ttgcttgttc aaacagctag caaatttgct tcagacatca ctcttgacta caaaggtaaa
     5641 gcagtaaatc taaaatcaat catgggtgtt atgagtcttg gtgttggtca aggtgcagat
     5701 gttactatct cagctgaagg tgcagatgct gaagatgcca ttgcagcaat tgaagaaaca
     5761 atgactaaag agggattggc ataagactat gacagaaatg cttaaaggaa ttgcagcctc
     5821 agacggcgtt gctgttgcta aagcatatct actagttcag ccggatttgt catttgagac
     5881 tgttacagtc gcagatacaa atgcagaaga agctcgcctt gatgttgcac tccaagctgc
     5941 acaagacgag ctttctgtta tccgtgaaaa tgcagtagaa agcttaggtg aagaagcagc
     6001 agccgttttt gatgcccatt taatggttct tgctgatcca gaaatgatca gccaggttaa
     6061 agaaacgatt cgcgcaaaac aaacgaatgc agaaacaggt cttaaagaag tgactgacat
     6121 gttcatcacc atctttgaag gcatggaaga taacccatac atgcaagaac gtgcagcgga
     6181 catccgcgac gttgcaaagc gtgtgttggc tcacctttta ggtgtaaaac ttccaaatcc
     6241 agctacaatc aatgaagaat caatcgttat cgcacacgat ttgacacctt cagatactgc
     6301 tcaacttaac aaacaatttg taaaagcatt tgttacaaat atcggtggtc gtacaagtca
     6361 ctcagctatc atggcacgta cacttgagat cgctgcggta cttggaacaa atgatattac
     6421 aaaacgtgtt aaagatggtg atgtgattgc cgttaatggt atcactggtg aagtgattat
     6481 cgatccaagc gaagatcaag tacttgcttt taaagaagct ggtgcggctt atgccaaaca
     6541 aaaagcagag tggtctctcc ttaaagatgc gcatactgaa acagctgatg gcaaacactt
     6601 tgaattggct gctaatatcg gtacgcctaa agacgttgaa ggtgttaatg acaatggcgc
     6661 tgaagctgtt ggcctttacc gtactgagtt cttgtacatg gattctcaag acttcccaac
     6721 tgaagacgaa caatacgaag cttacaaggc agtgcttgaa ggcatgaatg gcaaacctgt
     6781 tgtggttcgt acgatggata ttggtggcga caaggaactt ccttactttg accttccaaa
     6841 agaaatgaat ccattccttg gtttccgtgc tcttcgtatt tccatctctg aaactgggga
     6901 tgccatgttc cgcacacaaa tgcgtgcgct tcttcgtgcc tctgttcacg gacaacttcg
     6961 tattatgttc ccaatggttg cccttcttaa agaattccgt gctgcaaaag caatctttga
     7021 tgaagaaaaa gcaaacttgc ttgcagaagg cgttgcggtt gctgatgaca tccaagttgg
     7081 tatcatgatt gagattcctg cagctgctat gcttgcagac caatttgcta aggaagttga
     7141 tttcttctca attggaacaa acgaccttat ccaatacact atggcagcag atcgtatgaa
     7201 cgaacaagta tcataccttt accaaccata caacccatca atattacgtt tgatcaacaa
     7261 tgtgatcaaa gcagcgcacg ctgaaggtaa atgggcaggt atgtgtggtg agatggcagg
     7321 tgaccaacaa gctgttccac ttcttgtcgg aatgggcttg gatgagttct ctatgtcagc
     7381 aacttcagta cttcgtacgc gtagtttaat gaagaaactt gactctgcta agatggaaga
     7441 atatgcaaat cgtgcgctta cagaatgttc aacagcagaa gaagttcttg aactttctaa
     7501 agaatacgtt tctgaagatt aacgggttaa agagttggtt taaaccagct cttttttgct
     7561 agctaaaaat acaattactt tttggtgtga aatcatttga gttatcaaat aattatcaga
     7621 attttcagat atttagtgta gccaaatctt gaattatatc ggaaaagtga tagaataaga
     7681 ttactatcat aaaaggagat aaaacgttgg caaaacaata taaaaattta gtgaacggtg
     7741 aatggaaact atcagaaaac gagattacca tttacgcacc agcaacaggt gaagagttag
     7801 gatcagttcc agcgatgacg caggcagagg tagatgctgt ttacgcttca gctaaaaagg
     7861 ctctatcaga ttggcgcgcc ttgtcttatg tggaacgtgc agcttacctt cataaagcgg
     7921 ctgatatttt agtacgtgat gctgaaaaga tcggcgcgat tctttcaaaa gaagtagcca
     7981 aaggtcacaa ggcagctgtc agtgaagtta ttcgtaccgc tgaaatcatt aattatgcag
     8041 cagaagaagg gcttcgtatg gaaggtgaag ttcttgaagg tggtagcttc gaagccgcaa
     8101 gtaagaagaa gattgctatt gttcgtcgtg aaccagttgg tttagttctt gccatctcac
     8161 cttttaatta tcccgttaac ttggcaggtt ctaaaattgc tccagccctt attgcaggaa
     8221 atgttgttgc tcttaaacca ccaacacaag gctctatttc tggtttgtta ctagcagaag
     8281 cttttgcaga agctggtatt ccagcaggtg tctttaatac cattacaggg cgaggttctg
     8341 ttattgggga ttatatcgtt gagcacgaag cggttaactt tatcaacttt acaggttcta
     8401 ctccaattgg ggaaggaatt ggtaaattag cgggtatgcg accaattatg cttgagcttg
     8461 gtggtaagga ttctgctatc gttttggaag atgcagatct tgctttagca gcgaaaaata
     8521 ttgtagccgg tgcttttggt tactcaggac aacgttgtac agcggttaaa cgcgttcttg
     8581 tgatggacaa ggtggcggat caattggcgg ctgagattaa aacccttgtt gaaaaactaa
     8641 gtgtcggaat gcctgaagac gatgctgata ttacaccatt aattgataca tcagctgctg
     8701 attttgttga agggttgatt aaagatgcaa ctgataaggg agctactgct ttgacagcct
     8761 ttaatcgtga aggcaatctt atttcacccg ttctctttga tcatgtgaca actgacatgc
     8821 gtttggcgtg ggaagagccg ttcggcccag tattaccaat tattcgtgta accactgtag
     8881 aagaagccgt caagatttct aatgagtctg aatatggttt gcaagcttct atttttacaa
     8941 ctaatttccc aaaagctttt ggcattgctg agcaattaga agttggaact gttcacctta
     9001 acaataaaac acaacgtgga acagataatt tcccattctt aggcgctaaa aaatcaggtg
     9061 caggggtaca aggagttaaa tattctatcg aagctatgac aactgttaaa tctgttgtat
     9121 ttgatatcca gtaataaaaa aaaataaaca aaaagttagg ttaacttaac tttttgttta
     9181 tttttttaat gttttttgtt acaatagtct atacagatgg aggcaactat tgaaaaaatt
     9241 aaatgttatt cttgttggtt tattaagcat tctgatgttg agtttagcta ttgtgtttat
     9301 taatcgttgg aaactaaacg aagatagtca gcgtatagtt ttggctgaaa agaaaaaaaa
     9361 cacgtcagat ttagtgatca aagc
//
LOCUS       DAUGHY010000050         8915 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.050, whole genome shotgun sequence.
ACCESSION   DAUGHY010000050 DAUGHY010000000
VERSION     DAUGHY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.050"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            90..1010
                     /gene="lmb"
                     /locus_tag="VH752_001408"
     CDS             90..1010
                     /gene="lmb"
                     /locus_tag="VH752_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEQ8289752.1"
                     /translation="MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKNAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            1023..3494
                     /locus_tag="VH752_001409"
     CDS             1023..3494
                     /locus_tag="VH752_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEQ8289753.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYRFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YMMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQAD
                     KSNENQQPSEASKEEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQP
                     EGVQFYNKNGELVTYDIKTLQQINP"
     gene            3663..3863
                     /locus_tag="VH752_001410"
     CDS             3663..3863
                     /locus_tag="VH752_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ8289754.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(3946..4572)
                     /locus_tag="VH752_001411"
     CDS             complement(3946..4572)
                     /locus_tag="VH752_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289755.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTS
                     LSRVLAGFLQPKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYP
                     SQDLRDAFGIQEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCDACYMIEENRTQLFKHT"
     gene            complement(4556..5359)
                     /locus_tag="VH752_001412"
     CDS             complement(4556..5359)
                     /locus_tag="VH752_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289756.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIKQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGDGEQLQTEFARCLWRTLPQQDFLKGVTHDLRG"
     gene            complement(5371..6192)
                     /locus_tag="VH752_001413"
     CDS             complement(5371..6192)
                     /locus_tag="VH752_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289757.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVFLATVFGVLAGLGNSLID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(6189..7166)
                     /locus_tag="VH752_001414"
     CDS             complement(6189..7166)
                     /locus_tag="VH752_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289758.1"
                     /translation="MKRTTIIIIWKIIRCITLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKNVIQGHLGTSLVYRQPVSDIIRSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRVKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQRQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(7279..8907)
                     /locus_tag="VH752_001415"
     CDS             complement(7279..8907)
                     /locus_tag="VH752_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ8289759.1"
                     /translation="MSKYLKYFSIITLFLTGLILVACQQQKPQTKERQRKQRPKDEIV
                     VSMGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKTYHLSEDGLTWSF
                     DLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNGKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIPSNDVRGLSLPYVKK
                     GVITDSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDKTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHHPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESTK"
BASE COUNT         2926 a         1748 c         1774 g         2467 t
ORIGIN      
        1 aaaagatggt agttgccatt gacaaagcat tgataaagga gtaaaattaa ctggttaata
       61 actggttaaa ttataattga ggaggtacta tgaaaaaagg tttttttctc atggctatgg
      121 tcgtgagttt agtaatgata gcagggtgtg ataagtcagc aaaccccaaa cagcctacgc
      181 aaggcatgtc agttgtaacc agcttttacc caatgtatgc gatgacaaaa gaagtatctg
      241 gagacctcaa tgatgtgagg atgatccaat caggtgcagg cattcattcc tttgaaccgt
      301 ctgtaaatga tgtggcagct atttatgacg cggatttgtt tgtttaccat tcacatacct
      361 tagaagcttg ggcaagggat ctagacccta atttaaaaaa atcaaaggtt gatgtgtttg
      421 aagcgtcaaa acctctgaca ctagatagag tcaaagggct agaagatatg gaagtcacac
      481 aaggcattga ccctgcgaca ctttatgacc cacatacctg gacagatccc gttttagctg
      541 gtgaggaagc tgttaatatc gctaaagagc taggacgttt ggatcctaaa cacaaagaca
      601 gttacactaa aaatgctaag gctttcaaaa aagaagcaga gcaactaact gaagaataca
      661 ctcaaaaatt taaaaaggtg cgctcaaaaa cattcgtgac gcaacacacg gcattttctt
      721 atctggctaa acgattcggc ttgaaacaac ttggtatctc gggtatttct ccagagcaag
      781 agccctctcc tcgccaattg aaagaaattc aagactttgt caaagaatac aacgtcaaga
      841 ctatttttgc agaagacaat gtcaatccca aaattgctca tgctattgcg aaatcaacag
      901 gagctaaagt aaagacatta agtccacttg aagctgctcc aagcggaaac aagacatatc
      961 tagaaaatct tagagcaaat ttggaagtgc tctatcaaca gttgaagtaa aggagattat
     1021 tagtgaagaa aacatatggt tatatcggtt cagttgctgc cattttacta gctactcata
     1081 ttggaagtta ccaacttggt aagcatcata tgggtctagc aacaaaggac aatcagattg
     1141 cctatattga tgatagcaaa ggtaaggcaa aagcccctaa aacaaacaaa acgatggatc
     1201 aaatcagtgc tgaagaaggc atctctgctg aacagatcgt agtcaaaatt actgaccaag
     1261 gttatgtgac ctcacacggt gaccattatc atttttacaa tgggaaagtt ccttatgatg
     1321 cgattattag tgaagagttg ttgatgacgg atcctaatta ccgttttaaa caatcagacg
     1381 ttatcaatga aatcttagac ggttacgtta ttaaagtcaa tggcaactat tatgtttacc
     1441 tcaagccagg tagtaagcgc aaaaacattc gaaccaaaca acaaattgct gagcaagtag
     1501 ccaaaggaac taaagaagct aaagaaaaag gtttagctca agtggcccat ctcagtaaag
     1561 aagaagttgc ggcagtcaat gaagcaaaaa gacaaggacg ctatactaca gacgatggct
     1621 atatttttag tccgacagat atcattgatg atttaggaga tgcttattta gtacctcatg
     1681 gtaatcacta tcattatatt cctaaaaagg atttgtctcc aagtgagcta gctgctgcac
     1741 aagcctactg gagtcaaaaa caaggtcgag gtgctagacc gtctgattac cgcccgacac
     1801 cagccccagg tcgtaggaaa gccccaattc ctgatgtgac gcctaaccct ggacaaggtc
     1861 atcagccaga taacggtggc tatcatccag cgcctcctag gccaaatgat gcgtcacaaa
     1921 acaaacacca aagagatgag tttaaaggaa aaacctttaa ggaactttta gatcaactac
     1981 accgtcttga tttgaaatac cgtcatgtgg aagaagatgg gttgattttt gaaccgactc
     2041 aagtgatcaa atcaaacgct tttggatatg tggtgcctca tggagatcat tatcatatta
     2101 tcccaagaag tcagttatca cctcttgaaa tggaattagc agatcgatac ttagccggcc
     2161 aaactgagga caatgactca ggttcagatc actcaaaacc atcagataaa gaagtgacac
     2221 atacctttct tggtcatcgc atcaaagctt acggaaaagg cttagatggt aaaccatatg
     2281 atacgagtga tgcttatgtt tttagtaaag aatccattca ttcagtggat aaatcaggag
     2341 ttacagctaa acacggagat catttccact atataggatt tggagaactt gaacaatatg
     2401 agttggatga ggtcgctaac tgggtgaaag caaaaggtca agctgatgag cttgctgctg
     2461 ctttggatca ggaacaaggc aaagaaaaac cactctttga cactaaaaaa gtgagtcgca
     2521 aagtaacaaa agatggtaaa gtgggctata tgatgccaaa agatggcaag gactatttct
     2581 atgctcgtga tcaacttgat ttgactcaga ttgcctttgc cgaacaagaa ctaatgctta
     2641 aagataagaa acattaccgt tatgacattg ttgacacagg tattgagcca cgacttgctg
     2701 tagatgtgtc aagtctgccg atgcatgctg gtaatgctac ttacgatact ggaagttcgt
     2761 ttgttatccc tcatattgat catatccatg tcgttccgta ttcatggttg acgcgcgatc
     2821 agattgcaac aatcaagtat gtgatgcaac accccgaagt tcgtccggat gtatggtcta
     2881 agccagggca tgaagagtca ggttcggtca ttccaaatgt tacgcctctt gataaacgtg
     2941 ctggtatgcc aaactggcaa attatccatt ctgctgaaga agttcaaaaa gccctagcag
     3001 aaggtcgttt tgcaacacca gacggctata ttttcgatcc acgagatgtt ttggcaaaag
     3061 aaacttttgt atggaaagat ggctccttta gcatcccaag agcagatggc agttcattga
     3121 gaaccattaa taaatctgat ctatcccaag ctgagtggca acaagctcaa gagttattgg
     3181 caaagaaaaa cgctggtgat gctactgata cggataaacc caaagaaaag caacaggcag
     3241 ataagagcaa tgaaaaccaa cagccaagtg aagccagtaa agaagaagaa aaagaatcag
     3301 atgactttat agacagttta ccagactatg gtctagatag agcaacccta gaagatcata
     3361 tcaatcaatt agcacaaaaa gctaatatcg atcctaagta tctcattttc caaccagaag
     3421 gtgtccaatt ttataataaa aatggtgaat tggtaactta tgatatcaag acacttcaac
     3481 aaataaaccc ttaaccaaaa gaagatctca ttgttaaagc actgctttgt caaagcaagt
     3541 tacggtgatt ttgaagtcat tttatgtaac gagtagtgat aaaagttgga taatagcggt
     3601 tttcttttgc aaagaaatgg tatccatgtt agaataataa aaaaaagagg aggattctaa
     3661 tcatgtcaga agaaaaatta aaatcaaaaa ttgagcaagc atctggtggc ctaaaagaag
     3721 gtgctggtaa gttgacaggt gataaagagc tagaagcaaa aggatttgtt gaaaaaacaa
     3781 ttgctaaagg caaagaactt gcagacgatg caaaagaagc ggttgaaggt gctgtagatg
     3841 ccgttaagga aaaattaaaa taattaaaaa aaaccagctg taagctggtt tttttaatgg
     3901 tgtgattttg ttaggaaaag actcaatagg taataaccgt aggtgttagg tatgcttgaa
     3961 cagttgagta cgattctctt caatcatata gcacgcgtca caaagttttt ctaacatagc
     4021 aaaatcatga ctaataacga tgagacctaa atttctatcc tttacaatct ctaacaggct
     4081 tttccataca ctagcttgtg taatgctatc caacatagta gtcatttcat ctgcaataag
     4141 gtatttcgtc tctggatgta atgaacgcac aatcgaaaag cgttgcaatt cccctcccga
     4201 gagttcacta gggcgacgct ttagccattt ttcttgaatg ccaaaagcat ctcgcaaatc
     4261 ttgacttgga tagtaggctt cttccaaact ttttttcata ggccataaag gattcatggt
     4321 ttgttcagga tgttgttgga taagttgtac aggtctaaat gctttgttag gcaaatgact
     4381 gccatcaact aatacttcac cagatttagg ctgcaaaaaa ccggcaagca ctctggataa
     4441 actcgttttt ccacacccac tttgtccaaa tatccctaag atttgaccag gtgctacctc
     4501 taaatcaatc tccttaaaaa gccattgatc ttttttatga taaaagccaa gctttttagc
     4561 ctctaaggtc atgagtaact cctttcaaaa agtcttgctg agggagtgtg cgccataaac
     4621 atctcgcaaa ttccgtttgt aattgctccc catctccgct aaaaaagcta gctggggctg
     4681 tttcaatagc ttttccctct ttaaaaatag ttatacgatc cgcaatttga ctagctgcta
     4741 caatatcatg agtgataaat atgacgctta ttcctttatc tgcaaaggag cgtagttgat
     4801 ctaaaaccat ttgcagagca tctggatgta atccaggggt aggctcatcc gcaataatca
     4861 aagaaaccgt atcactaata cacgttgtaa acaaaacacg tcggagcatt ccgccagaaa
     4921 gttggaaagg atacaagtca ccatcacttt cttttaaacc aaactgttga aacaatcctt
     4981 cttgagtagc cttagcattt tctgagatac ccaagcgcac ctgatgcttg actttcatag
     5041 atggatctaa ataattaacg gattgtggaa tcaacgtcat ttcttttcct cgcaactgtt
     5101 tgatgcgttt agaagttagt gattgaccac gataaatcat atctcctgtt acagctgcat
     5161 ttttaggaag aatatccata atagcatgtg ctaataaact cttgcctgaa ccactagcac
     5221 ctataatagc taataactca ccttttttaa cttctaaatt cagcgcttgg atcggtgtgg
     5281 attgaaatgg ttttaaaaat cttccgtatt gagtgaaggt gatggagagg tctttaatgc
     5341 ttaataatgt ttctgtcata tctctacctc ctaaaaatga tccgtctgag ggtaaaagag
     5401 tttctttaaa gattctccga tagtatcaaa ggcattgaca accaaaataa gataaaggcc
     5461 tggaaaaatc accaaccacc aattgccaag agagatatgc ttagctgcct ctgacaaaat
     5521 gataccaacc gaaggttgtt cggcagaaag gccaaatcct aagaaagtca tggatgcttc
     5581 atgcaagatg acgtgcggaa ataagaggat aaacccaatg aaaatttgag aagcaatcaa
     5641 aggcaggata tgatgcctca caatataata aggcgttttt cccatgcttt tagagagctg
     5701 gacaaaggct ttattcttta gatcatagac ttcattgcgg ataagccttg ctagagaggg
     5761 ccaatgggta acagctgttg caatgataac cccttgagcc cctttcccaa caacaaaaga
     5821 aatgagaatc ataaaaatca aatgaggcat accaataaac aaatcaacta accatgctat
     5881 tattttatca ataaggctat ttcctaaacc tgcaagcact ccaaaaacgg tcgcaagaaa
     5941 gactcccata agggcaccta ataagccgac ttgtaaagag aaataaagcc ctttaatcgt
     6001 tctgacaaac atatccctac ctaaaccatc tgtcccaaaa agatggttta aggaaggggc
     6061 gaggttgcgt aaagctgcat tggtttccaa aggcgtccta tagaaataaa ggtttaaggc
     6121 tagaatacta agaatgagag aaatggcgat acctagttgc cataaaacca tcgttcgacg
     6181 tttcaatatc atacttttct ccttaactgt ggattgatga tgctatttaa aatgtccgca
     6241 ataagattgc ccgcaaaaac aaataatgtc cctatcatca cgatagctag aagtagcggt
     6301 gtatcacttt taagtcctgc ttcagtgagg gtagacccta agccaggata tgagaaaact
     6361 tgctcagcaa gaacagatcc tccaaacaat tctccaaaat aggaaaaatg cagtgtaata
     6421 gctggtacga tagcatttct aagacaatga tttttaaaaa tttgccgctg tgtttcccca
     6481 cgcgctctgg caaataagac atattcacta gaaagcaccg acatcatttt agttctcgta
     6541 tgaagagtga cattggcaat gcctagaata cttagcgtga aaacaggtaa cataaggtgc
     6601 ttaacccgat cagctaacgt aatatcttga ctcaaagtcc caatcgggga agaaatacca
     6661 atcgggaacc accccagctg gacagaaaag attaataaaa aaatgaggcc aatccaaaac
     6721 gttggtactg atatctgaag gtaagaaaac cacctgacaa ctcggtcaag taatttccct
     6781 tgatggaaag cggataacgt tcctaagata aatccaataa gacccgataa gatccaagag
     6841 agtcccataa gtatgaaaga agcacctgcc cgtgatctaa taatatcact aacaggttgc
     6901 cgataaacga gcgaggtccc taaatgtccc tgtatcacat ttttcaacca aataaaatat
     6961 tggactagag ctggcttatc caagccatag tggtgagcaa tcgctttgta ctgagcaggg
     7021 gttagtgatg tgtcataatt gacacttgcc atgacgggat ctactggaga ttgttttaac
     7081 aaaacgaagg tcaaaacaga taccccaaaa ataagtgtga tacatctgat gattttccag
     7141 atgataataa tggtagtacg tttcacaatg aaatctcctt aatcgataag aaaaggagcc
     7201 gaggaaacga tcagcacagt tttgtgtatc ttgttttctc agactccaaa cttaaagtcg
     7261 ctgtattaac taaacgcgtt acttagttga ttcatcccaa gtccactcgg caatgttagt
     7321 caataatgac caatcatgac catgactgtg gacgccttgt ttacctacat tgatacgttt
     7381 atcaccaata taagtatggt taaggctcac caaccataca tttggcaaat ctccaagagt
     7441 agaagcacct gttttgccat cccactgcgc taacttccaa tattcgttag ctttgtcaag
     7501 gtcagaagat gtcattgctt tgtcaaggta cttagtcacg gtaggattgt tataaaacgt
     7561 aatattggtc caacctttac ctgctaggct tggatgatgt gattcataaa attgctgcgc
     7621 gtgatgacgt cctccggcat aaagtaaggc tgagtcatgt gacttcgttg ccatttcatc
     7681 ccagttacta gctttgagtt taatagtaat ccctagggct ttggcttgct ctgctacttc
     7741 aacggctaag ttcgctcgca attgatcatt agtagggtag tacagatcaa acgctgcatc
     7801 aaggtcacct tttttacggc taccgtctgc ttgttctttc catcccgctt ttgtcaacaa
     7861 ttgcttagct ttagctactt tattatcttt aatggctgtt tttggattcc aaaatggtgt
     7921 tttatcaata attgaataag ctggtttacc ataaccattt aaaaccgtat cgagaacttt
     7981 ttgtctattt aaaccaatag tcaaggcttt gcggattgct ggatcgctag tgacatcatt
     8041 tcctacagga taaccatcag gagaatcagt gatgacgcct tttttcacat aaggtaatga
     8101 taagccgcgc acatcatttg atggaatgtc aaggaggcga gtgcctttga ctttcttact
     8161 agcaagttct ggtgtcgcgt agatcatatc aacatcacca gattctaaag cagctagtgc
     8221 tgtgttttca tcaagtaaga cccaagtcca ctttttaaag tatggttttt tcccatgcca
     8281 ataagggtta cgaacaaaaa tagcttgttc tccagcctta tattctttta ccatgtaagg
     8341 tcctgaaccg ataggattgc tcttatactt accattgtaa tgttttttag ggacgattgg
     8401 gatttcagtc aactgtgctg taaatgtcga atgcgcctca gtcaaatgga tattgacctg
     8461 atttttccca actacttcaa cgttcttgat gaaggttaga tcccaagcct ttccatctgc
     8521 tttcaacata tcataagtaa acttaacatc atcagcagta acaggctcac catttgagaa
     8581 tttaaaatca tcatgcaagt caaacgacca agtcagccca tcttcagaga gatggtatgt
     8641 tttagcaagc tctcctttta tatctagttc aggagaacgt ttcaatagag tgctatgagt
     8701 gatattccct tcattgtgga ctccataacg gtcctttgga tcgaattcat gagggagctt
     8761 tgcccccata gaaacgacaa tttcgtcttt tggacgttgt ttgcgctgac gttcttttgt
     8821 ttggggcttt tgttgttgac atgcaactaa aataagccca gtcaaaaata acgtgataat
     8881 agagaagtat tttaggtatt ttgacacaat caaat
//
LOCUS       DAUGHY010000051         8759 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.051, whole genome shotgun sequence.
ACCESSION   DAUGHY010000051 DAUGHY010000000
VERSION     DAUGHY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.051"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            16..855
                     /gene="purR"
                     /locus_tag="VH752_001416"
     CDS             16..855
                     /gene="purR"
                     /locus_tag="VH752_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEQ8289760.1"
                     /translation="MKLRRSERMVVISNYLINNPYKLTSLNTFATKYEAAKSSISEDI
                     AIIKKAFEEANIGDIDTLTGASGGVIFTPSISETEARTIVEDLCQRLSENDRILPGGY
                     IYLSDLLSTPKILQNIGRIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVR
                     RDLKITEGSTVSVNYASASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISL
                     LTEFDSTLVGVAVFAENAQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKER
                     EWT"
     gene            846..3467
                     /locus_tag="VH752_001417"
     CDS             846..3467
                     /locus_tag="VH752_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEQ8289761.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASGET
                     KASNTHDDSLPKPETIQEAKATIDAVEKTLSQQKAELTELATALTKTTAEINHLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKA
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKHNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEALAEQAAARVTALVAKKAHLQYLKDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQEALAALQAKQSSLEATIATTEHQLTLLKTLANEKEYRHLDED
                     IATVPDLQVAPSLTGVKPLSYSKVETTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESR"
     gene            3675..4088
                     /gene="rpsL"
                     /locus_tag="VH752_001418"
     CDS             3675..4088
                     /gene="rpsL"
                     /locus_tag="VH752_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEQ8289762.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            4109..4579
                     /gene="rpsG"
                     /locus_tag="VH752_001419"
     CDS             4109..4579
                     /gene="rpsG"
                     /locus_tag="VH752_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEQ8289763.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFSAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            4946..7024
                     /gene="fusA"
                     /locus_tag="VH752_001420"
     CDS             4946..7024
                     /gene="fusA"
                     /locus_tag="VH752_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEQ8289764.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            7372..8382
                     /gene="gap"
                     /locus_tag="VH752_001421"
     CDS             7372..8382
                     /gene="gap"
                     /locus_tag="VH752_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEQ8289765.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINAAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(8608..8724)
                     /locus_tag="VH752_001422"
     CDS             complement(8608..8724)
                     /locus_tag="VH752_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEQ8289766.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
BASE COUNT         2880 a         1700 c         1781 g         2398 t
ORIGIN      
        1 aaaaatgggg aaactatgaa attaagacga agtgaacgta tggttgtcat ttcaaattat
       61 ttgattaaca atccgtataa attaactagt ttaaacacct ttgcaactaa atatgaggcc
      121 gcaaaatctt ctatctcaga agatatcgct attatcaaga aagcttttga agaagctaac
      181 attggtgata tcgatacact tacaggagca agtggaggcg taatttttac ccccagtatt
      241 tcagaaacag aagcgcgtac cattgttgaa gacctgtgtc aaaggttatc agagaatgat
      301 cgcatcttac ctggaggcta tatctatttg tcagacttgc tgagtacacc taaaatttta
      361 caaaatattg gtcgtattat tgccaatgcc tttaaaggag aaaagattga cgctgtgatg
      421 acagttgcaa ccaaaggagt tcctttagca aatgcagtgg ctaatatttt aagtgttcct
      481 tttgtcattg ttagacgtga tttaaaaata acagaaggat cgacagtgtc tgtcaattat
      541 gcgagtgctt ctagtgatcg tattgaaaag atgtttctgt caaagcgtag tttgaaaccc
      601 aatagtcgtg tattgattgt agatgatttc ttgaagggcg gggggacgat tacaggaatg
      661 atcagtctct tgacagaatt tgacagcact ttagttggtg ttgctgtgtt tgcagaaaac
      721 gctcaatcag agcgtgagca aatgaccttc aaatcattgc tgaaagtttc agagattgat
      781 gtcaaaaaca acaatgttgt tgtagaagtt ggtaatattt tcgataaatg gaaaaaggag
      841 agagaatgga cttagaacaa acgaagccaa accaagttaa gcagaaaatt gctttaacct
      901 caacaattgc tttattgagt gccagtgtag gcgtatctca ccaagtcaaa gcagatgata
      961 gagcctcagg agaaacgaag gcgagtaata ctcacgacga tagtttacca aaaccagaaa
     1021 caattcaaga ggcaaaggca actattgatg cagttgaaaa aactctcagt caacaaaaag
     1081 cagaactgac agagcttgct accgctctga caaaaactac tgctgaaatc aaccacttaa
     1141 aagagcagca agataatgaa caaaaagctt taacctctgc acaagaaatt tacactaata
     1201 ctcttgcaag tagtgaggag acgctattag cccaaggagc cgaacatcaa agagagttaa
     1261 cagctactga aacagagctt cataatgctc aagcagatca acattcaaaa gagactgcat
     1321 tgtcagaaca aaaagctagc atttcagcag aaactactcg agctcaagat ttagtggaac
     1381 aagtcaaaac gtctgaacaa aatattgcta agctcaatgc tatgattagc aatcctgatg
     1441 ctatcactaa agcagctcaa acggctaatg ataatacaaa agcattaagc tcagaattgg
     1501 agaaggctaa agctgactta gaaaatcaaa aagctaaagt taaaaagcaa ttgactgaag
     1561 agttggcagc tcagaaagct gctctagcag aaaaagaggc agaacttagt cgtcttaaat
     1621 cctcagctcc gtctactcaa gatagcattg tgggtaataa taccatgaaa gcaccgcaag
     1681 gctatcctct tgaagaactt aaaaaattag aagctagtgg ttatattgga tcagctagtt
     1741 acaataatta ttacaaagag catgcagatc aaattattgc caaagctagt ccaggtaatc
     1801 aattaaatca ataccaagat attccagcag atcgtaatcg ctttgttgat cccgataatt
     1861 tgacaccaga agtgcaaaat gagctagcgc agtttgcagc tcacatgatt aatagtgtaa
     1921 gaagacaatt aggtctacca ccagttactg ttacagcagg atcacaagaa tttgcaagat
     1981 tacttagtac cagctataag aaaactcatg gtaatacaag accatcattt gtctacggac
     2041 agccaggggt atcagggcat tatggtgttg ggcctcatga taaaactatt attgaagact
     2101 ctgccggagc gtcagggctc attcgaaatg atgataacat gtacgagaat atcggtgctt
     2161 ttaacgatgt gcatactgtg aatggtatta aacgtggtat ttatgacagt atcaagtata
     2221 tgctctttac agatcattta cacggaaata catacggcca tgctattaac tttttacgtg
     2281 tagataaaca taaccctaat gcgcctgttt accttggatt ttcaaccagc aatgtaggat
     2341 ctttgaatga acactttgta atgtttccag agtctaacat tgctaaccat caacgcttta
     2401 ataagacccc tataaaagcc gttggaagta caaaagatta tgcccaaaga gtaggcactg
     2461 tatctgatac tattgcagcg atcaaaggaa aagtaagctc attagaaaat cgtttgtcgg
     2521 ctattcatca agaagctgat attatggcag cccaagctaa agtaagtcaa cttcaaggta
     2581 aattagcaag cacacttaag cagtcagaca gcttaaacct ccaagtgaga caattaaatg
     2641 atactaaagg ttctttgaga acagaattac tagcagctaa agcaaaacaa gcacaactcg
     2701 aagctactcg tgatcaatca ttagctaagc tagcatcgtt gaaagccgca ctgcaccaga
     2761 cagaagcctt agcagagcaa gccgcagcca gagtgacagc actggtggct aaaaaagctc
     2821 atttgcaata cttaaaggac tttaaattga atcctaaccg ccttcaagtg atacgtgagc
     2881 gcattgataa tactaagcaa gatttggcta aaactacctc atctttgtta aatgcacaag
     2941 aagctttagc agccttacaa gctaaacaaa gcagtctaga agctactatt gctaccacag
     3001 aacaccagtt gactttgctt aaaaccttag ctaatgaaaa ggaatatcgc cacttagacg
     3061 aagatatagc tactgtgcct gatttgcaag tagctccatc tcttacgggc gtaaaaccgc
     3121 tatcatatag taaggtagag actactccgc ttgttcaaga aatggttaaa gaaacgaaac
     3181 aactattaga agcttcagca agattagctg ctgaaaatac aagtcttgta gcagaagcgc
     3241 ttgttggcca aacctctgaa atggtagcaa gtaatgccat tgtgtctaaa atcacatctt
     3301 cgattactca gccctcatct aagacatctt atggctcagg atcttctaca acgagcaatc
     3361 tcatttctga tgttgatgaa agtactcaaa gagctcttaa agcaggagtc gtcatgttgg
     3421 cagctgtcgg cctcacagga tttaggttcc gtaaggaatc taggtgatgc attcgtcata
     3481 agcaattgca tcaaaggaaa aattctattt gacatgaagt accgtctgtg ttactatatt
     3541 agacggtact ttttactttt ggtctctcaa aagtgtacag agacgtgctg acaattgttg
     3601 caaaaagtgc accggatatg ggctgtcacc aagtgctata tcaaccaaaa ataaaaaaac
     3661 acaggagaat gtagatgcct acaattaacc agttggtacg taaaccacgt aaatctaaaa
     3721 ttgaaaaatc agattcacca gctttgaaca ttggttacaa cagtcacaaa aaagttcaaa
     3781 ctaaaatggc tgccccacaa aaacgtggcg ttgctactcg tgttggaaca atgacaccta
     3841 aaaaacctaa ctcagccctt cgtaaattcg ctcgtgtacg tttgagcaac cttattgaag
     3901 ttacagctta catcccaggt atcggacaca acttgcaaga gcacagtgtt gttcttattc
     3961 gtggcggacg tgtaaaagac cttccagggg tacgttacca tatcgttcgt ggcgcacttg
     4021 atactgcagg tgttgctgac cgtaaacaag gccgttctaa atacggtgcg aaacgtccaa
     4081 aaggataata gaagggggat aagagaaaat gagtcgtaaa aatcaagcgc ctaaacgcga
     4141 agtattacca gatccattat ataactcaaa aatcgtaaca cgtcttatca accgtgttat
     4201 gcttgacggt aaacgtggta cagctgctac tatcgtttac gatgctttca gcgctatcaa
     4261 agaagcaaca ggaaatgacg ctcttgaagt atttgaaaca gctatggaca acatcatgcc
     4321 tgtacttgaa gtacgcgcac gccgtgtcgg tggttctaac taccaagtcc cagttgaagt
     4381 tcgtccagaa cgtcgtacaa cacttggact tcgttggttg gtaaacgcat cacgagctcg
     4441 tggtgaacac actatgaaag atcgtcttgc taaagaaatc atggatgctg caaacaacac
     4501 aggtgcatca gttaagaaac gtgaagacac tcacaaaatg gctgaagcta accgtgcctt
     4561 tgctcacttc cgttggtaat ataagatatt ttagcgtcaa acaaatgcta gccaaaatag
     4621 ggaaattgac gcagaaactt cagtttctag gtaattttat ctttgtggca gacatttgta
     4681 gcttgaactc aattaataag atacgaaggc gtatagaacg caaagtgaaa ataggaaatt
     4741 tgacgaagaa acctttgttt ctaggaaagt ttatcttttt cacacagcgt ttagcctgag
     4801 ttcaatgaat aagataccaa gccttataca ggcaatagat attatcttta ccaacaaata
     4861 tttagataac gggtaggtcc tgcctatccg tttttgctaa atagtgctat aatgaaagag
     4921 taaactaaaa aaataggaga aacacatggc tcgcgaattt tcacttgcaa aaactcgtaa
     4981 tatcggtatc atggctcacg tcgatgctgg taaaacaact acaacagagc gtatccttta
     5041 ctacactggt aaaattcata aaatcggtga aacacacgaa ggcgcatcac aaatggactg
     5101 gatggagcaa gagcaagaac gtggtatcac tattacttct gccgctacaa cagctcaatg
     5161 ggatggtcac cgcgttaaca tcatcgacac accaggacac gtggacttca ccatcgaagt
     5221 acaacgttct ctccgtgttc ttgatggtgc tgtaaccgtt cttgactcac aatcaggtgt
     5281 tgagcctcaa actgaaacag tttggcgtca agcaactgaa tacggtgttc cacgtatcgt
     5341 tttcgccaac aaaatggata aaatcggtgc tgacttcctt tactcagtac aaacccttca
     5401 tgaccgtctt caagcaaatg cacacccaat ccaattgcca atcggtgcag aagatgattt
     5461 ccgtggtatc attgacttga tcaagatgaa agcagaaatc tataccaacg accttggtac
     5521 agatatcctt gaagaagata ttccagaaga atacttagaa caagctcaag aataccgtga
     5581 aaaattaatc gaagcagttg ctgaaactga tgaagacttg atgatgaaat accttgaagg
     5641 tgaagaaatc actaacgacg aattgattgc tggtatccgt aaagcaacaa tcaacgttga
     5701 attcttccca gtactttgtg gttctgcctt caaaaacaaa ggtgttcaat tgatgcttga
     5761 tgcggttatc gcataccttc caagcccact tgacatccct gcaatcaaag gtgttaaccc
     5821 ggatacagat gctgaagaag aacgtccagc atctgatgaa gagccatttg cagctcttgc
     5881 cttcaagatc atgactgacc cattcgtagg tcgtttgaca ttcttccgtg tttactcagg
     5941 tgttcttaat agcggttctt acgttatgaa cacatcaaaa ggtaaacgtg agcgtatcgg
     6001 acgtatcctt caaatgcacg caaacagccg tcaagaaatt gaaacagttt acgctggtga
     6061 catcgctgcc gctgttggtt tgaaagacac aacaactggt gactcattga ctgatgaaaa
     6121 agcaaaagtt attcttgagt caatcgaagt tccagaacca gttatccagt tgatggttga
     6181 gccaaaatct aaagcagacc aagacaagat gggtgttgcc cttcaaaaac ttgctgaaga
     6241 agatccaaca ttccgtgttg aaacaaacgt tgaaactggt gaaacagtta tcgctggtat
     6301 gggtgaactt caccttgatg tccttgttga ccgtatgaaa cgtgagttca aagttgaagc
     6361 taacgttggt gctcctcaag tatcttaccg tgaaacattc cgtgcttcta cacaagctcg
     6421 tggattcttc aaacgccaat ctggtggtaa aggtcaattc ggtgatgttt ggattgaatt
     6481 tacaccaaat gaagaaggta aaggtttcga attcgaaaat gctatcgttg gtggtgtggt
     6541 tcctcgtgaa tttatcccag ctgttgaaaa agggttgatc gaatctatgg caaacggtgt
     6601 tcttgctggt tacccaatgg ttgacgttaa agctaaactt tacgatggtt cataccacga
     6661 tgtcgactca tctgaaactg ccttcaagat cgcagcatct cttgctctta aagaagcagc
     6721 taaatcagca caaccagcta tccttgagcc gatgatgctt gtaacgatta cagctccaga
     6781 agacaatctt ggagacgtta tgggacacgt gacagctcgt cgtggacgcg ttgatggtat
     6841 ggaagcacat ggtaacagcc aaatcgttcg tgcttatgtt ccacttgctg aaatgttcgg
     6901 ttatgcaaca gttcttcgtt ctgctactca aggacgtggt acattcatga tggtatttga
     6961 ccactatgaa gatgttccta agtcagttca agaagaaatc attaagaaaa acaaaggtga
     7021 ataaccacca ctaagaggtt gaggataact atcctaacct cttttttagt gtaacagaac
     7081 gttatagtac agtttgaagt tttggaaatc tttcccaata aaaagataaa ttacagaata
     7141 gaacatagaa aaaagaaagc cctttcctat atttggtggg ttttgaaggc tagaagaaag
     7201 aagctgccct ttatgaaaat agctaattta tttgcatttt ttctgaaata gttatataat
     7261 agttctgttg aaaggttgtt gcagatgact gtaagttaat cttttcacaa taggtaggga
     7321 gcattccctc taaataatat tcttttgatt ttcataagga ggaaatcact aatggtagtt
     7381 aaagttggta ttaacggttt cggtcgtatc ggacgtcttg cattccgccg tattcaaaac
     7441 atcgaaggtg ttgaagtaac tcgtatcaac gaccttacag atccaaatat gcttgcacac
     7501 ttgttgaaat acgatacaac tcaaggtcgt tttgatggaa cagttgaagt taaagaaggt
     7561 ggatttgaag taaacggaaa cttcattaaa gtttctgctg aacgtgatcc agaaaacatc
     7621 gactgggcaa ctgatggggt tgaaatcgtt cttgaagcaa ctggtttctt tgctaaaaaa
     7681 gaagcagctg aaaaacactt acatgctaac ggtgctaaaa aagttgttat cacagctcct
     7741 ggtggaaacg atgttaaaac agttgttttc aacactaacc acgacattct tgacggtact
     7801 gaaacagtta tctcaggtgc ttcatgtact acaaactgtt tagctcctat ggctaaagct
     7861 cttcacgatg cattcggtat tcaaaaaggt cttatgacta caatccacgc ttacactggt
     7921 gaccaaatga tccttgacgg accacaccgt ggtggtgacc ttcgtcgtgc acgcgctggt
     7981 gctgcaaata tcgttcctaa ctcaactggt gctgctaaag ctatcggtct tgttatccca
     8041 gaacttaacg gtaaacttga cggtgctgca caacgtgttc ctgttccaac tggatcagta
     8101 actgagttgg ttgtaactct tgacaaaaac gtttctgttg acgaaatcaa cgctgctatg
     8161 aaagctgctt caaacgatag cttcggttac actgaagatc caatcgtttc ttcagatatc
     8221 gtaggcgtat catacggttc attgtttgac gcaactcaaa ctaaagtaat ggaagttgac
     8281 ggatcacaat tggttaaagt tgtatcatgg tatgacaacg aaatgtctta cactgctcaa
     8341 cttgtacgta ctcttgagta cttcgcaaaa attgctaaat aattagttat aacgaaagag
     8401 agcttggttt atgctgagtt ctttttcgta tactttaaac actaccttaa atgtatgatt
     8461 ttaacgtttt gaactattct agactctagc ataaaagata ttttaaaata gttcccttaa
     8521 taaactgtat acaaaaaggc taagatggaa ggggtgtctt agcctttttt tgtctgactt
     8581 aaatttggag agataaaaag taaaatctta ttttttaccg taacgtttgt actcgtgaat
     8641 aatcttcaga gcacgttttc tggttttaat attgggactt ttcagtcttc tgtaagctga
     8701 cgataagtca gttttttctg ccatattatc ctcctaaata taaattatta acgttaaca
//
LOCUS       DAUGHY010000052         8745 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.052, whole genome shotgun sequence.
ACCESSION   DAUGHY010000052 DAUGHY010000000
VERSION     DAUGHY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.052"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            30..623
                     /locus_tag="VH752_001423"
     CDS             30..623
                     /locus_tag="VH752_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ8289767.1"
                     /translation="MTKMYYDENPDSLHDIHEVKVELLNHPFTFLTDSGVFSKKMVDF
                     GSQVLLETLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNRALDLARKNATN
                     NQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFLVVNGDLTIVIQ
                     KKQGAPSAKAKMATIFGNVEILRKDKGYYVLRSIKQA"
     gene            1274..1663
                     /locus_tag="VH752_001424"
     CDS             1274..1663
                     /locus_tag="VH752_001424"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEQ8289768.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFAPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTDLI"
     gene            1757..2809
                     /locus_tag="VH752_001425"
     CDS             1757..2809
                     /locus_tag="VH752_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ8289769.1"
                     /translation="MNKKFIGLGLASVAVLSLAACGNRGASKGGASGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            2948..4480
                     /locus_tag="VH752_001426"
     CDS             2948..4480
                     /locus_tag="VH752_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289770.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIMKNLVKEGKSIILITHKLDEIRAVADRVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKKLEVDENRGVPAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNNEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVSPENTNKQELGILMAGGSIHKEEG
                     HV"
     gene            4473..5537
                     /locus_tag="VH752_001427"
     CDS             4473..5537
                     /locus_tag="VH752_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289771.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMIAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            5539..6495
                     /locus_tag="VH752_001428"
     CDS             5539..6495
                     /locus_tag="VH752_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289772.1"
                     /translation="MSLVTIFALLVSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(6708..8426)
                     /locus_tag="VH752_001429"
     CDS             complement(6708..8426)
                     /locus_tag="VH752_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ8289773.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSAGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGTTMFNVLTGFKFIAEKIQEFEE
                     KQNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMNKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
     gene            complement(8602..>8745)
                     /locus_tag="VH752_001430"
     CDS             complement(8602..>8745)
                     /locus_tag="VH752_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantothenic acid transporter pant"
                     /protein_id="HEQ8289774.1"
                     /translation="FIFFATVFDGNIKALLTAIISSNAIVEMIISAIITFVLIPTLSR
                     LKR"
BASE COUNT         2685 a         1419 c         1786 g         2855 t
ORIGIN      
        1 aaaagtacct taaaaagata ggaaaaatga tgacaaagat gtattatgat gaaaatccag
       61 atagcctaca cgatattcat gaagtaaaag tagagctgct taatcatccc tttacttttt
      121 tgacggattc aggtgtcttt tcaaagaaaa tggttgattt cggtagtcaa gtattattag
      181 agacactaaa tttcaaggag aacgaacgtg tactggactt aggttgcggc tatggtcctc
      241 ttgggattag tctagccaag gttcaaaggg tagacgcaac cttagtggac atcaataatc
      301 gtgctcttga tttagctaga aaaaatgcaa ctaataacca agtggcagta actatttttc
      361 aatcaaatat ttatgaaaat attagtggcc attttgagca tattattagt aatcctccta
      421 ttagagcagg aaagagagta gttcatagca ttattgaaaa aagcatagat tttttagtgg
      481 ttaatggtga cttaacgatt gtgattcaaa aaaaacaagg agctcctagc gctaaagcaa
      541 aaatggcaac tatttttggt aatgttgaga tactaaggaa agataaaggt tattatgtat
      601 taagaagtat aaagcaagcc taatttaaat aatcaacaat tgctcattta aattaggttt
      661 gcttaaatag taaagagtta tttttaaatt atagataact aatattatac tagggtagcc
      721 cctctactcg agtaaccttt tttaatgtat tttaactgaa aaaggctttc ataattttaa
      781 aaaaatgtta aaataggtgt ataacaactg attaccaaga gattattatc attatcttct
      841 aaggtgtcat tgttatggtg acaatcaacc atacgctact aaagataggt agcgttcgat
      901 cacaggttga tcaagtgatt gatacagata tttataattt tacaaatatc tgtatcaatc
      961 catgttgaat agtctatgct tctaaggtgt cgacgttaat gtactgacgg gaccgttata
     1021 gtctttcttt ttggagaaac gtcggatgcc agtgaactag ttgaagttaa agatgttaat
     1081 tcaaacagtg ctgaagagat tgatatcgtt ttgaacggtc gtttacttca aagcaaagaa
     1141 attaccagtc tatcggagat gatataagtg ctaaagttag atcacgttaa ttatctatca
     1201 agtcgggtgc atatttcgtt aaaatatcta caggggtttc aatagatgta gctattatga
     1261 aaggtgatgt taaatgggga ctactgattt agttagttgt gctgtacaag ctagtgagta
     1321 tgcttatgtc ccttactctc attttccagt aggagcagct ttaaaaacaa aagatggaac
     1381 gatctatact ggctgtaata ttgaaaatgt tagttttggt ctgacaaatt gtggagagcg
     1441 aacagctata tttaaagcca tatctgatgg tcataaagaa ttggttgaaa ttgctattta
     1501 tggagaaact atgcaacctg tatctccttg tggggcttgt cgtcaggtaa tggcagaatt
     1561 ttttgctcct tcttctttag tcactttaat tgctaaaaat ggtcaaactg ttgaaatgac
     1621 tgttggagat ttactcccct attcttttac agatttaata taatctgctt taatttctgt
     1681 tatctagccg tcttggcttt atatttactt tcagcaactt atttgctaga aatctttttt
     1741 aggagggttc atattcatga acaagaaatt tattggtctt ggtttagcgt cagtggctgt
     1801 gctgagttta gctgcttgtg gtaatcgtgg tgcttctaaa ggtggggcat caggaaaaac
     1861 tgatttaaaa gttgcaatgg ttaccgatac tggtggtgta gatgacaaat cattcaacca
     1921 atcagcatgg gaaggcctgc aatcttgggg taaagaaatg ggccttcaaa aaggaacagg
     1981 tttcgattat tttcaatcta caagtgaatc tgagtatgca actaatcttg atacagcagt
     2041 ttcaggaggg tatcaactga tttatggtat cggctttgca ttgaaagatg ctattgctaa
     2101 agcagctgga gataatgaag gagttaagtt tgttattatc gatgatatta tcgaaggaaa
     2161 agataatgta gccagtgtta cctttgctga ccatgaagct gcttatcttg caggaattgc
     2221 agctgcaaaa acaacaaaaa caaaaacagt tggtttcgtg ggcggtatgg aaggaactgt
     2281 cataactcga tttgaaaaag gttttgaagc aggagttaag tctgttgacg atacaatcca
     2341 agttaaagtt gattatgctg gatcatttgg tgacgctgca aaaggaaaaa caatcgcagc
     2401 agctcagtat gcagcaggtg ctgatgttat ttaccaggca gcaggaggca ctggagcagg
     2461 tgtatttaat gaagcaaaag ctattaatga aaaacgtagt gaagctgata aagtttgggt
     2521 tattggtgtt gaccgtgatc aaaaagacga aggaaaatac acttctaaag atggcaaaga
     2581 agcaaacttt gtacttgcat catcaatcaa agaagttggt aaagctgttc agttaatcaa
     2641 caagcaagta gcagataaaa aattccctgg aggaaaaaca actgtctatg gtctaaaaga
     2701 tggcggtgtt gaaatcgcaa ctacaaatgt ttcaaaagaa gctgttaaag ctattaaaga
     2761 agcgaaagca aaaattaaat ctggtgacat taaagttcct gaaaaatagt gtttaagaac
     2821 aagaagcgcg acctttgtcg gtcgctcttt ttaaactgag ataattttta tctcagtaaa
     2881 gtttgtgata ggtgatagat cataggcttt actgaaataa aagattgcat tgaaaggaaa
     2941 tgatcctatg acacagcatg tcattgaaat gagagaaatc acgaaaaaat ttggtgattt
     3001 tgttgcaaat gaccacatta acttaaatgt cagaaaaggt gagattcatg ccttattggg
     3061 tgaaaatggg gcaggtaaat caactttgat gaatatgcta gctggtctct tagagcctac
     3121 tagtggagaa attgtcatta atgacaagcc agttcaaatt gattcgccat ctaaatcagc
     3181 taagcttggt atcggaatgg tacatcaaca tttcatgtta gtagaagcct tcacggttgc
     3241 tgagaatatt attttaggaa atgaagtagt aaaaaatggg tgcttagatc ttaaccaagc
     3301 atcgaaagat attaaggtac tatccgaaaa atatggcctt gcaatcaatc catcagctaa
     3361 agtttctgat atttcagtag gagcacaaca acgtgttgag attttgaaaa ccttgtatcg
     3421 tggtgcagat attcttatct ttgatgagcc aactgccgtt ttaacacctg cagaaattaa
     3481 agaattgatg actatcatga agaatctcgt gaaagaagga aaatcaatta ttttaattac
     3541 ccataaactg gacgagattc gtgctgtcgc cgaccgtgtc acagttattc gtcgtggtaa
     3601 gagtatcgaa acagttgatg tggcaggagc aacttcacaa gatttagctg agatgatggt
     3661 gggacgctcg gtttccttta caactagcaa aaaagctgct gaaccaaaag atgtggtctt
     3721 atccatcaaa aaacttgaag tagatgaaaa tagaggggtt ccagcagtta aggggctatc
     3781 tctagatgtc cgagctggtg aaattgtagg gattgctgga attgacggaa atggtcaatc
     3841 tgagttaatt caagctatta caggtttacg taaagtcaaa tctggaagta ttatgattaa
     3901 aaacaatgaa gtcactcatt tgtcttcacg caaaattact gagttatcag tgggtcacgt
     3961 gccagaagat cgtcatcgag atggtttgat actagatctt tctttagctg aaaatacagc
     4021 cttacaaacc tattacaaac aacctctaag tcaaaatgga atcttaaatt atactaaaat
     4081 taatgactat gctcgtcaat taatgaaaga atttgatgtt cgcggagcca atgagctagt
     4141 accagctaga ggattttctg gtggtaacca acaaaaggcc attattgctc gtgaagtgga
     4201 ccgtgatcca gatttactta ttgttagcca accaacacgt ggtcttgacg taggagctat
     4261 tgaatatatc cataagcgtt tgattaaaga acgtgataaa ggtaaagctg tcctcgttgt
     4321 tagttttgaa cttgatgaaa tattgaattt atctgatcga attgctgtta ttcatgatgg
     4381 taaaattcaa ggtatcgtat cgccagaaaa taccaataaa caagaattgg gtattttaat
     4441 ggcaggtggt tcaattcata aggaggaagg tcatgtctaa aaatgcgcaa aaaattgctg
     4501 taccattgat ttctgtcttg ctaggatttt tacttggcgc tattattatg gttatctttg
     4561 gttatgatcc tatctggggg tatgaaggtt tattccaaat tgcctttggt agtgttaaaa
     4621 atattggaga aatattccgt tcaatggggc cattgatttt aatagcgctt ggctttactg
     4681 ttgctagccg tgcaggcttc tttaacgttg gtttgtctgg acaggcttta gctggatgga
     4741 tttcagcagg ttggtttgca ctcttaaatc cagatatgcc tcgtccatta ttgattctta
     4801 tgacagctct tatcggaatg attgctggtg gtattgctgg agctattcca gggatacttc
     4861 gtgcttatct tggtacaagt gaagttatcg ttacgattat gatgaactac atcattttat
     4921 atgtcggaaa tgctattgtt caacgtggtt atcctgagag tgtcaaacaa agtattgatt
     4981 caactattca agttagtgat aatgctagtt atcaaacgca ttggttatca gccctaacta
     5041 ataactctcg tattaatatt ggtattttct ttgccattat tgccattgcc ctaatctggt
     5101 tcttgcttaa caaaactact ttaggttttg agattcgttc agttggtctc aacccacatg
     5161 cttcagaata cgcagggatg tcatcaaaac gtactatcat tttatcaatg attatctctg
     5221 gagctcttgc aggtcttgga ggagtagttg aaggtctagg aacctttgaa aatgtttttg
     5281 tacaaggatc ttctttagca gttggttttg acggtatggc agttagctta ttagcagcaa
     5341 attcaccgct tggaatattt ttctcatcat tcctatttgg tgtcttaaat attggtgctc
     5401 caggaatgaa tattgcaggc attccaccag aacttgttaa agtagtaact gcatcaatca
     5461 ttttctttgt tggttcacat tacctcattg aacgttacat tattcgccct aaaaaattag
     5521 tgaaaggtgg taaataagat gagtttagta acaatttttg ccttattggt gtcatctatg
     5581 cttatttatg caacaccact cattttcaca agtattggag gaactttctc agagcgctca
     5641 ggcgttgtta atgttggttt agaaggtatc atggtcatgg gagcgttctc tgggattgtt
     5701 ttcaacttag agtttgctga aacatttgga aaagcaacgc cttggatagc cgttttagtc
     5761 ggtggtattg ttggtttgat tttttctttg attcacgctg tagcaaccat caactttcga
     5821 gctgaccaca ttgtcagtgg tacagtgttg aacttgttag caccttcttt tgctgtcttc
     5881 ttggttaaag ctatgtacgg taagggacaa acagacaaca ttcaacaatc ttttgggaag
     5941 tttgatttcc caggtttatc acaaattcct gtgattggtg atattttctt taaaaatact
     6001 agccttattg gttacttcgc cattgctttt tcgttctttg cttggttttt gctttataaa
     6061 accaggtttg gtctgcgttt acgatcagtt ggggaacacc ctcaggcagc tgacacactt
     6121 ggtattaacg tctatttgat gaaatactat ggtgtgatga tctcaggttt tcttggtgga
     6181 ataggtggag ctgtttatgc tcagtcaatc tccgttaact ttgctgtaac aactatctta
     6241 ggtcctggat ttattgcttt ggcagctatg atctttggta aatggaatcc agttggcgcc
     6301 atgctatcta gtcttttctt tggcttatcg caaagtttag ccgttattgg tgctcaatta
     6361 ccattactag aaaagattcc aacggtttac ttacagattg caccttatat ggtaacgatt
     6421 attattttag ctgctttctt tggtcaagca gttgcaccaa aagctgatgg aatcaactat
     6481 attaaatcta aataacgtta tattagtaag cctcttagtg aggtttaaca gcactcctaa
     6541 agcaagatag ttaggtctag ctttaagggt gcttttttgg gacttaccgc aagtttggat
     6601 tgccagtcac catcacaata gaagcaagaa tagactaaaa agaactaagt cagcggctta
     6661 gttctttttg cttgtctttc gtttttactg atagctctta agttgtttta gccaacaaag
     6721 gtattgattt ctgtttcaat gttagcaatt ttttcttgag cgatgtcaag agtatcacct
     6781 attgtggcaa tatagaactt gatttttggt tcagttcctg aagggcgaac tgcaatccat
     6841 gaatcatcag ctaacgtgta cttcaaaaca ttacttggag gagttgttaa gttagatatt
     6901 gttccatcat ttttagtagc ggtttgtttt tggaaatctt ctaataatac aatatcagta
     6961 ttgttaaatt gttttggacc attttcacgg aatttgttca taatcttctt aatttcagca
     7021 gctccatcca cacctgaaag tgttactgaa atcgtttttt cagcaaaata gccatattct
     7081 ttgtaaattt catcaatacc atctgcaagt gttaaaccac gcgaacggta ataagccgcg
     7141 atctcagcaa ccaaaagaac tgcttgaata gcgtctttat cacggacaaa tggtttaata
     7201 agatatccga agctttcttc aaaaccaaac atataggtgt ggttttgttt ttcttcaaac
     7261 tcttgaattt tctcagcgat gaatttgaat ccagttaaaa cgttaaacat tgtagtgcca
     7321 taactttcgg caatttttgt tactaattct gttgatacga ttgatttagc gagagcagca
     7381 ttttctggaa gtgttcctgc tgttttatga gcttccagga tgtatttcgc aataatcgct
     7441 ccaatttgat ttccagaaag atttttataa gaaccatctg gttggcgaat ttcaacgcct
     7501 aggcggtctg catcagggtc ggtagctact aagacatctg catcaacttc tcgtccgagt
     7561 tcttcagcaa gagcaaaggc agcttggctt tcagggttag gcgatttaac agtagaaaag
     7621 tctggatctg ccttagcttg tgattcgact acaacaacag attcaaaacc ggcttgagca
     7681 agggcacggc gtgtcaacat ctcaccagtt ccgtggagag gagtatagac aattttcatg
     7741 tcttttccaa agttattgat taaatcttga ttgatattaa catctttaac ctctctaaga
     7801 tattcaatat caacatcttc accgataact tgaataagac ccgctgattt gctttcatct
     7861 aaatcagcaa gttcaactgc aaatggattt tcaatagcac ggataaagtc tgttagtgca
     7921 tcagcatcag ctggtggtag ttgacctccg tcttgtccat agactttata accattgaaa
     7981 ggtgctggat tgtggcttgc agtaaccata ataccagcat aggcatttaa atggcgaaca
     8041 gcaaaagaga gttctggtgt tggacgaaga gcttcaaata cgtaagattt aattccatgc
     8101 tgtgccaaga cctgtgcaga ctcaaaagca aattctggag agaagtggcg cgaatcataa
     8161 gcgatggcta caccacgttt tttagcttcc tctcccttag tttcgattaa ttttgctaat
     8221 ccttcggttg cttgacggac aacataaata ttaatacgat ttgtaccagc accaatataa
     8281 ccacgcatac cagcagtacc aaattctaag tttgtgtaaa aagcatcttc ttttgttttt
     8341 tcatccattg atagtaattc ttgacgaaga tagtcaggaa gctgctcaaa atctaaccat
     8401 ttttgaaaat tttctgtgta agtcattgaa atactccttc aatatttata aacgctttca
     8461 ttatatcatt ttgaaggaaa aaaaacacta attatttgca gctgtttaca aaactttttc
     8521 catattttca agtagctaat gtaagaaagt taaaataacc aagtttttaa aagagaaaaa
     8581 cttggttatt gtgaaagcta attacctttt tagtcttgat agtgttggaa taagtacaaa
     8641 ggtaataata gctgagatga tcatctcaac aatagcgttt gatgagatga tggctgttag
     8701 tagtgcttta atatttccat caaaaactgt cgcgaaaaaa ataaa
//
LOCUS       DAUGHY010000053         8582 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.053, whole genome shotgun sequence.
ACCESSION   DAUGHY010000053 DAUGHY010000000
VERSION     DAUGHY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.053"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..1969
                     /locus_tag="VH752_001431"
     CDS             <1..1969
                     /locus_tag="VH752_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEQ8289775.1"
                     /translation="TSFTLLVTDVQADQVDVQFLGVNDFHGALDNTGTAYTPSGKIPN
                     AGTAAQLGAYMDDAEIDFKQANQDGTSIRVQAGDMVGASPANSALLQDEPTVKVFNKM
                     KFEYGTLGNHEFDEGLDEFNRIMTGQAPDPESTINDITKQYEHEASHQTIVIANVIDK
                     KTNDIPYGWKPYAIKDIAINDKIVKIGFIGVVTTEIPNLVLKQNYEHYQFLDVAETIA
                     KYAKELQEQHVHAIVVLAHIPATSKDGVVDHEMATVMEKVNQIYPEHSIDIIFAGHNH
                     QYTNGTIGKTRIVQALSQGKAYADVRGTLDTDTNDFIKTPSANVVAVAPGIKTENSDI
                     KAIINHANDIVKTVTERKIGTATNSSTISKTENIDKESPVGNLVTTAQLTIAKKTFPT
                     VDFAMTNNGGIRSDLVVKNDRTITWGAAQAVQPFGNILQVIQMTGQHIYDVLNQQYDE
                     NQTYFLQMSGLTYTYTDNDPKNSDTPFKIVKVYKDNGEEINLTTTYTVVVNDFLYGGG
                     DGFSAFKKAKLVGAINTDTEAFITYITNLQASGKTVNATIKGVKNYVTSNLERSTKIN
                     SAGKHSIISKVFRNRDGNIVSSEVISDLLTSTENTNNSFGKKETTTNKNTISNSTLPI
                     TGDNYKMSPIMTILALISLGGLNAFIKKRKS"
     gene            complement(2051..2461)
                     /locus_tag="VH752_001432"
     CDS             complement(2051..2461)
                     /locus_tag="VH752_001432"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ8289776.1"
                     /translation="MIREIITDHFLLQQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLNLTGLTAQICQHELDHLEGILI"
     gene            3175..3252
                     /locus_tag="VH752_001433"
                     /pseudo
     CDS             3175..3252
                     /locus_tag="VH752_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3350..5227)
                     /locus_tag="VH752_001434"
     CDS             complement(3350..5227)
                     /locus_tag="VH752_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ8289777.1"
                     /translation="MSHFLVEKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRLGFDGDHSPFSKANDYKIAYLTQDPEFNDASSVLDTVLSSDVKAIQLIRQ
                     YELLMANYTEDKQETLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKTVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDATNLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFTDLKKEVHQDSSADKLEMAFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDIGDTIRVGYFSQQFKDMD
                     ETKRVINYLQEVADEVKTSVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLTVLENFLANFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRIFYGNYSDYLDEKAFEKETVEADLAKAIVTEEMPLPPKEEKKRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYGQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
     gene            complement(5224..6432)
                     /locus_tag="VH752_001435"
     CDS             complement(5224..6432)
                     /locus_tag="VH752_001435"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEQ8289778.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQQEAGLNHLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDH
                     IEALDQALAIHNKYDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTI
                     EAFVREELAT"
     gene            complement(6557..7405)
                     /locus_tag="VH752_001436"
     CDS             complement(6557..7405)
                     /locus_tag="VH752_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ8289779.1"
                     /translation="MKLAVITDSTATLPTDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMADSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDGTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVSIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            complement(7405..7785)
                     /locus_tag="VH752_001437"
     CDS             complement(7405..7785)
                     /locus_tag="VH752_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEQ8289780.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            complement(7939..8189)
                     /locus_tag="VH752_001438"
                     /pseudo
     CDS             complement(7939..8189)
                     /locus_tag="VH752_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8414..>8582)
                     /locus_tag="VH752_001439"
     CDS             complement(8414..>8582)
                     /locus_tag="VH752_001439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEQ8289781.1"
                     /translation="KIKQAIDNGDITRDTVVVVLKNGRFFDDMLLHEEVSNRRAVIEE
                     RGEEALMKLDK"
BASE COUNT         2690 a         1780 c         1376 g         2736 t
ORIGIN      
        1 gactagtttt actctattag ttacagatgt tcaagcagat caagttgatg tgcaattcct
       61 tggcgtcaat gattttcacg gcgctcttga taataccgga acagcttaca caccaagtgg
      121 taaaatacca aatgctggga cggctgctca attaggtgct tatatggatg acgctgagat
      181 agacttcaag caagcaaatc aagacggaac aagtatacgt gttcaagctg gagatatggt
      241 cggagccagt cctgctaact ctgcactttt acaagatgag cctactgtaa aagtctttaa
      301 caaaatgaaa tttgaatatg gcactcttgg taatcatgaa tttgacgaag gactagatga
      361 atttaaccgt atcatgacag gtcaagcgcc tgatcctgaa tcaacaatta atgatatcac
      421 caaacaatat gagcacgaag cttcgcatca aaccatcgtc attgctaatg tcattgataa
      481 aaaaactaat gatatcccct atggttggaa accttatgct ataaaagaca tagccattaa
      541 tgacaaaatc gttaagattg gcttcattgg tgttgtgact acagagattc caaatctcgt
      601 tttaaagcaa aactatgaac actatcaatt tttagatgta gctgaaacca ttgccaaata
      661 tgctaaagaa ctgcaagaac aacatgttca tgctattgtg gttttagctc atattcctgc
      721 aacaagtaaa gatggtgttg ttgatcatga aatggctacg gttatggaaa aagtgaacca
      781 aatctatccc gaacatagca ttgatattat ttttgcagga cataatcatc aatacactaa
      841 tggaactatc ggtaaaacac gtatcgttca agccctctct caaggaaaag cttatgcaga
      901 tgtccgtggt acgctagata ctgataccaa tgattttatt aaaactccat cagcaaatgt
      961 tgttgctgta gcaccaggta tcaaaacaga aaattcagat atcaaagcta taataaatca
     1021 tgctaatgat attgttaaaa cagttactga acgaaaaatc ggaactgcaa ctaattcttc
     1081 aactatttct aaaacagaaa atattgataa agaatcccct gtcggtaact tagtaacaac
     1141 ggctcagctt actattgcta agaaaacttt tccaactgtt gactttgcta tgaccaataa
     1201 tggtggtatt cgaagtgacc tagttgtcaa aaatgaccgg accatcacct ggggagctgc
     1261 acaggctgta caaccatttg gtaatatcct tcaagtcatt caaatgactg gtcaacacat
     1321 ttacgatgtc ctaaatcagc aatacgatga aaaccagacc tattttcttc aaatgtcagg
     1381 tttaacatac acttatacag ataatgatcc taagaactct gataccccct tcaagatagt
     1441 taaggtttat aaagacaatg gtgaagaaat taacttaaca actacttaca ccgttgttgt
     1501 caacgacttt ctttatggtg gcggtgatgg cttttcagca tttaaaaaag ctaaattagt
     1561 cggagctatt aacacagata ctgaagcttt catcacatat atcacaaatt tacaagcatc
     1621 aggtaaaact gttaatgcta ctatcaaagg ggttaaaaat tatgtaactt caaaccttga
     1681 aagatcaaca aaaattaata gtgctggcaa acacagtatc attagtaagg tttttagaaa
     1741 tcgtgatggc aatatagtgt ctagtgaagt catttcagac cttttgactt ctactgaaaa
     1801 cactaataac agctttggca aaaaagagac aacaacaaac aaaaatacta tctctaattc
     1861 cactcttcca ataacagggg acaattataa aatgtctcct attatgacaa tccttgcctt
     1921 gataagctta ggtgggctaa acgctttcat taaaaaaaga aaatcctagc gtttactagt
     1981 aattgatcat ccttatataa tcaggcattt tcgccaaaaa aacctaaagt gtcctactct
     2041 aggttttttg ttaaattaaa ataccttcta gatgatctaa ttcgtgctgg catatttgag
     2101 cagttaggcc tgttaagttt aaacgctttg gtcgccaatt atggtccaaa tactcaacag
     2161 taatcttatc ataccgctgt gttttgcgat agccagataa tgacaaacaa gattctttag
     2221 tttggtaaat tcccttttta gaaaccatca caggattaaa catcaccaaa tcaataaatc
     2281 ccatactcac aataacaatg cgcttttgtt caccaatcat attggcagcc atcccaagac
     2341 attcctgtct ataaaaggct aaggtatctt gtaaatcttg gccaatccat agatcttctt
     2401 tcttggcgac ttgcgctttt tgctgtaata aaaaatgatc tgttatgatt tctctaatca
     2461 ttcttatctc cttaaacctt ctttttaatt cataaactac atgttcaatg gactattatc
     2521 accatatgat agacttagta tcatctgttt aaaacatcag gtgattcctt atttcaagat
     2581 atcttgggca actttatgaa attctgcaat atgagtacca aataaaaatt gacctgcatc
     2641 agcagattgt tgctaagttc tcatcacaat attatcgaat ttactgtctg cttcttcaaa
     2701 tgtctaagag agatagttat tgctttgtga gattttcaaa ccagatactg ctttctcttg
     2761 catttacagc cttgataggt ccaaacaaaa cacctgctgt ttgaaatgct ttaatagctg
     2821 atatgttgtt aatagttagt attataaatt ttctgaatat ttctgtcaag tttttcagtt
     2881 gacttaactt ttttacttga tttttcctca cgtaataata gaaaagaagc gccataatgt
     2941 aaactacacc ccaaacgtta gataaaaaat ctaacgtttg gggtgttttt gtatgacatt
     3001 aagctatgaa gataagctag acatttagcg cttaaaaact gagggaactc cgtttgaaag
     3061 aagcatctct gcaaagaaga agacaaaaac cattataaag aatggacaaa gacgagttct
     3121 gattagattt tcttttaaga acggctaaat tgactcgttc tacctactat tatcatcgat
     3181 tattgcaaca ataaacgtat caaaataaaa cgaaaagaac ttagtcctct acaatacaga
     3241 actaaatcct ttacttcaaa tagagtagcc aacttttggg gctcagtgca agagcacttc
     3301 ttttctatta tcacttgttt attataaaca taacattacc atagtatact tatatgtcta
     3361 gtccactcaa atactcaaac cgttcatacg ccaataataa atcattattg cgctgatcga
     3421 gttccttttg taattgagct aactggccat aatcactaac tacagttaac atttggtttt
     3481 caatttcttc aatattagcc tcaatagttg ctattttatc ttctatctga gcccattctt
     3541 gtttttcaag gtaggacatg cgctttttct cctctttcgg aggaagtggc atttcttcag
     3601 tgactattgc tttagctaag tcagcctcaa ctgtttcttt ttcaaaagct ttttcatcaa
     3661 ggtaatcact gtaattccca taaaaaatac gaatatcgcc ttcttcaaag gctaaaatct
     3721 tagtggctac tttgtctagg aaataacgat cgtgactaac cgtaatgact ggtcctgcaa
     3781 agttagctaa aaagttttct aagaccgtca aggtggcaat gtctaaatca tttgttggct
     3841 catcaaggag aagaacattt ggcttttcaa tgagcagttt tagcaagtag agccgttttt
     3901 tctccccacc agataattta gcaatcagcg tgccatgact tgatctcgga aaaagaaatt
     3961 gctctaaaag ctcactgata ctcgttgttc ccacgcttgt tttaacctca tcagccactt
     4021 cttgaaggta attaatgacc cgtttggtct catccatatc tttgaactgc tgagagaaat
     4081 aacctacacg aatagtatct ccaatatcaa gcttcccaga agttggtttg agatcaccgt
     4141 tgatgatatt taacagggta gattttccga cgccgttatc tccaacaatt ccaatacgat
     4201 ctttattttg aataatcaag ttaaaatcct taatcaactg tcggtcgcca taagcaaaag
     4261 aaaggtcttc aaagtgaatc accttcttcc caattcggct cgtttcgaaa gccatctcca
     4321 atttatctgc agaactgtct tggtgaactt cttttttcaa gtcagtgaaa cgattgattc
     4381 tagcctgctg tttggttgca cgcgcctgtg gctgcgtcct catccaagct aattcttgtt
     4441 tatagagttg cttcttcttg tgtaaattag tagcatctcg ttcgtcttgt tctgctttta
     4501 agcgcacata gtcttggtaa ttgccttgat attctgtcag tcccgcttta tccaattcaa
     4561 aaatacgggt agccacatga tcaaggaaat aacggtcatg ggtaataaaa aggacggttt
     4621 ttttagctgt cttgagatag gttgttaacc aagcgatggt gtcaatatcg agatggttgg
     4681 tcggctcatc caacaagagt aagtcagcag ctcctagcaa tacttgagct aactggacac
     4741 gtcttctcat accacctgat aggtctccca ctttttgttc caaatccgtg atgcctaatt
     4801 ttgataggac tgttttgaca tcactttcaa tagaccaagc atctaagcga tccatttcag
     4861 acatgaggga ctctaaggtc tcttgtttat cttcagtata attcgccatc aatagttcat
     4921 actgacgaat cagctgaatc gccttaacat cagatgacaa taccgtatct aaaacagagg
     4981 atgcatcgtt aaattcagga tcttgtgtta agtaagcgat tttgtaatca ttggctttag
     5041 aaaatggtga atggtctccg tcaaaaccaa gccttcccga caagacatct aaaagagttg
     5101 tttttcctgt tccattaacc ccaataatcc caatacggtc aaagtcatga ataataaagg
     5161 aaatatcctg aaagactgtt ttatctccca ccgtttttgt caatttctcc accaaaaaat
     5221 ggctcatgtt gctaattcct ctctaacaaa tgcttcaatt gtagtgaaat cattatctag
     5281 acgtccctct acaatcgcta attcaacttt ctcaagcata agccctagct gaggacctgg
     5341 tttcatccca aaatccttaa tcaggtgaga accattaagg acgatatcgt atttgttatg
     5401 aatggctagc gcttgatcta aagcttcaat gtggtccatg tcaacagata agctttgagc
     5461 ttttcggagg ccttccacta gacttgccat ttccttgccg tactggtaaa tatccaattt
     5521 ttcaaaagat ctttcctgac gaattctata aagagcaatc aactttgtga cataacgctg
     5581 aaaatcattt gaagtcttcc atgcttttag gaagctttta ggatcctcaa tggctaaact
     5641 aatcatcaca taagcccaag cttggtggtg atcactaaag gtaaagttat ctttcaaaga
     5701 aactatcaga tgattcaagc ctgcttcctg ctgcttcaag cctggtaaat agtcataagc
     5761 ctgacaagca atcatggcag aaatcccttt tcgccagtgc ggtgccatca ataatttatc
     5821 aaattcagta aaagaacgtt ccacagaaat cttttccaat agaggtgaat ggctcctcat
     5881 agcttcaaaa gttgcagctt caatgtcaaa atccagactc gccgcaaatc gaaagcctct
     5941 cataatgcgt agtgcatctt cttcaaaacg ttcctctgct ttacccactg ctcgtaaccg
     6001 tttttgctcc aaatcaatga gtcctctaaa tttatcaata acttgaccat tttcatccaa
     6061 ggccaaggca ttgacggtaa agtctcttcg ttttaagtct tcctctaaag agcgcacaaa
     6121 agaaacctga ctaggacggc gatagtcaac atagacatct tctgtcctaa aggttgtgat
     6181 ttcatactca ccgccatttt caagcacgag cactgtacca tgttcaatgc caacatcgac
     6241 agttctgtta aaaatcgctt tagtctcttc aggataggaa ctagttgcaa tatctacatc
     6301 atgaatggga cgctcgagta aaacatctct gacgcttcct cccacaaagt aggcttcata
     6361 accagcttct tttattttag tcaatatggg taaagccttc tgaaactcag aaggcattgt
     6421 cattaatttc atacgttgtt ttctaaccta tcatccaact agcccaatct aaagtgtgat
     6481 taggctactt gggttctatc acattttgtt attgttttag tgattcttgc aattaactat
     6541 tttttctaat cgacaattaa agcctcggtg ttacgccaaa tgcaatggct ccttctccta
     6601 aatgagtagc aatcaccgct ccaaaatgaa cctcttcaat gtcatattga taaccactgt
     6661 cttgtaagag tcgtttcaaa taatcagctt tgtcttgtgc ttttgagtga ataatagaca
     6721 cattatactg accgtctgct atcaagtcat ttaaaatctc aaccaagcgt ttcatagctt
     6781 tcttttctgt tctcactttt tcataaacaa cgatttttcc ctcttcatca aagcgtaaaa
     6841 taggtttaat actcaaaaga tttccaagaa gagcagaccc atttgacagg cgtccgcctt
     6901 taaccagatg attaaggtca tcaaccataa taaaggctgt cgtcccatca atctgttctt
     6961 gcagcttgtt taaaatagct tgaaatgtca ttccttgacg tgaccaatct agcacatttt
     7021 ttaccatact acctaaagga gcagatgtga tcttactgtc tggaaatgcc atttcaatct
     7081 caggatgttc ttcagctaaa aattgaatat tttgccaaaa gcctgagatt cctccagcta
     7141 agaacaagcc aataacatgt gtataaccct tagaagacaa aagtcctaaa agattatcta
     7201 gttcagataa acttggctga cttgtcttgg gaagattttg ggagtcagcc atcttttgat
     7261 aaaaatcatc tatactcaaa tttcgacctt caaaataggt ctcatcgtca atgataactg
     7321 gtatatcaag gctaaaaata gctttatctt gtttcagatc tgttggtagt gttgctgtac
     7381 tgtcagtaat tactgctaat ttcattaatt tttaaactcc aaatgaatac ctggacgatc
     7441 caaggcaatt tctgtgactt cataagtcaa tcgcttaacc atgtctagca aaggagcagc
     7501 taaggattca acttcctctt gcgtaaattc gcttggttta tccactaaac gatctaaaat
     7561 acgaaccatt tgtgagatga ccccactaat aacaaattct tctttgacaa taacaaattg
     7621 caatacagat acaattactg tttctttttg ctgctcatct ttatcaatca actgaaaagt
     7681 cacttcaaaa ttggtctctg gtgttccgtt ttctttttcc cactctaaat ttctagcatc
     7741 ataatggtac tggttgacaa attctttttc acgaactaat tgcatttttt attctccttg
     7801 gtatcaatat gatccattat agcatagatt tgaaaattat taaagtagcc cctaaactac
     7861 tattttcgca taacgtttta aacagtgcta tctctcgaac agatatctat tttttcacct
     7921 atttatattt agtcgtcctt atactgacat tatatcaaaa aaacacccgt tagtggtctt
     7981 gtactgcacc tcaattaaaa cagaaccact ggtcaggtaa gttttaagtc caggataaaa
     8041 aggtttatca ccaaggcctc atcatgctta gccaagtatt tcgaactagc ttgtcttagc
     8101 tctagtcgct caaacgtaaa tcctaggcgt tcaatccgcc gcaaaacttc tccaactaac
     8161 ccatacttaa acccataggg cttaatcatc gctccgccct tcttctttta tccattataa
     8221 aataaaaacg ttttaacaga caaaaaatag gaaagtacca gactattgga tttatttttt
     8281 tatctcaaac tccattaacg atgttgagtt aaaggcattc aaaacaacat agatcgtaag
     8341 ctaacaagat tttttatcaa ttgaacctat cgtccccagt tatatcacga cctcttctgg
     8401 atgaggtctt tttttatttg tctaatttca tcaacgcttc ttcccctctt tcttctatca
     8461 cagctctcct attgctcact tcctcatgca gcaacatatc atcaaaaaac cgtccatttt
     8521 taagcacaac cactactgta tctcttgtga tatctccatt gtcaatcgct tgtttaattt
     8581 ta
//
LOCUS       DAUGHY010000054         8273 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.054, whole genome shotgun sequence.
ACCESSION   DAUGHY010000054 DAUGHY010000000
VERSION     DAUGHY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.054"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..724)
                     /locus_tag="VH752_001440"
     CDS             complement(<1..724)
                     /locus_tag="VH752_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289782.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGGNVPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYVVIP"
     gene            complement(727..1659)
                     /locus_tag="VH752_001441"
     CDS             complement(727..1659)
                     /locus_tag="VH752_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ8289783.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMTAPLLVYTDLKVVTQHLAICHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASATGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLTPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            complement(1649..2803)
                     /locus_tag="VH752_001442"
     CDS             complement(1649..2803)
                     /locus_tag="VH752_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEQ8289784.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQHQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSKPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTCFIAYGTQTQPSRLTTADSKVRAYFQTFDIREKDYYLILGR
                     FVPENNYETAIKEFMASSTKRDLVIICNHEDNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWSK
                     QKGQLAELINQVDAGFDSDRLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
     gene            complement(2922..3776)
                     /gene="rfbD"
                     /locus_tag="VH752_001443"
     CDS             complement(2922..3776)
                     /gene="rfbD"
                     /locus_tag="VH752_001443"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEQ8289785.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
                     FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            complement(3914..4252)
                     /locus_tag="VH752_001444"
     CDS             complement(3914..4252)
                     /locus_tag="VH752_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEQ8289786.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            complement(4488..5597)
                     /gene="rpoD"
                     /locus_tag="VH752_001445"
     CDS             complement(4488..5597)
                     /gene="rpoD"
                     /locus_tag="VH752_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEQ8289787.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            complement(5606..7420)
                     /gene="dnaG"
                     /locus_tag="VH752_001446"
     CDS             complement(5606..7420)
                     /gene="dnaG"
                     /locus_tag="VH752_001446"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ8289788.1"
                     /translation="MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADMEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQK
                     RQGQIAQAVSNLVTLPIPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTL
                     EILYQRLKQQGYITSYDLSEMSEEINRAYYNVLEENLPKEVALGEIDDILSKRAKLLA
                     ERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
     gene            7550..7912
                     /gene="mscL"
                     /locus_tag="VH752_001447"
     CDS             7550..7912
                     /gene="mscL"
                     /locus_tag="VH752_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEQ8289789.1"
                     /translation="MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLIL
                     NPALKAANVENITQLSWNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAA
                     APTQEELLTEIRDLLAQK"
     gene            complement(8040..8216)
                     /locus_tag="VH752_001448"
     CDS             complement(8040..8216)
                     /locus_tag="VH752_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEQ8289790.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT         2490 a         1672 c         1454 g         2657 t
ORIGIN      
        1 atggaataac aacgtaaaac caattggttg acatctgcca aatcgttacg ttggctctgt
       61 caatcaataa ataacgaaaa tcttgaatca tttgtgctac tggatttagc atcaacaact
      121 ttgccgccaa agggtggcta tctaaaacaa aagtgatcgg ataaatgatt ggagtggcat
      181 acatacctgc ttgtaatagt acttcccaga cttgagctaa gtcacgataa taaacaaaga
      241 cattcgacaa taacaatgca attccaagca ctaaaacaac taattctata aaaagaaaaa
      301 gagagagata agcataccct gatatagtca caccatttat caacgcaaaa attaaaacaa
      361 caaccaaatt aataagaaaa ttaattaaag ctcctaacac tgccgaaaaa acaatgatgt
      421 gcttagaaaa gttcaatttt cgcaacaagt ctccccgaga tacaatagat accattccca
      481 tgctagttgc ttctgaaaaa aaagaccaga tgacatttgc caataaaagc gctactggaa
      541 aatgaggtac atttccaccc aggcgaagaa aacgaataaa taccaagtac ataatcgtaa
      601 acatcataag cggttttaag atagaccata gatagccaat tgcgctccct tgatagcgta
      661 atttaaaatc cgtttttacc atttctctca gtaaaatacg gtttttcttt gttaaaaaat
      721 tcattcttac tttctcctgt aaccaaataa ggtaacaact aaactacgaa aaataaaggt
      781 gtgaaagatg cgattttttc taaaaccgta ccttttcaag gtagctaagc gtttggtaaa
      841 aggcatatcc aataaagaaa cataggccgt taccaattca tggtcatttg gttttaatgg
      901 taaatctaat agtaattgag cctgtttttg gcttgaagta atcaaccacc aatatttgtt
      961 gactaaatga tggggggtta accaattttt catgcgttta gaccaagttc tagctcccaa
     1021 cacgttagca tcgtgctgac ggtataattc tgttggaata tcaaggtaaa ctaatttccc
     1081 agtagcagaa gctagtaaag ctaaatacca gtcatgcatc aataaaccgt cacaagtggt
     1141 ccattcctca gccagagcat gagtaatcat cattgtgccg cctgttacgg tattttcggt
     1201 taattcttga agtaggcttg tgtttgcatg accagattgg gtcttaatca tactgtcatg
     1261 gcaaatagct aaatgttggg ttaccacttt gaggtctgta taaaccaata aaggagctgt
     1321 catttcatgt ttttgagcct ccaataaggt cacttccagt ttattgtcta accatatatc
     1381 atcttggtca ctgaaaaaat agacatctgc tttttggtgc tttaagaggg tgtaaaaatt
     1441 cttaataact cccagatttt ctgtctgccc ctcattgatc cactgaattc gtttgtcttc
     1501 cttgacaaat gtgcgaataa tatcctgggt gccatcagtt gatccatcat ctctaatcaa
     1561 taaggtccag tcgttaactg tttgcctttg gatactttga atttgttctg ctaagaagcg
     1621 ttctccattg taggtggata gtaaaatatt aatgttcatt taaaaataaa gcctcgtact
     1681 cccctacaat tttttcccaa gtgtaatgtt cttgaataat agctttggct tctttgccca
     1741 gacgatcact atcaaaacct gcatcaactt ggttaatcag ctctgctaat tgcccttttt
     1801 gcttggacca atacagcgca gcagacttag ccaccgactg gttaaaatca actcctaaaa
     1861 ccaaattaag attggtatgg gctagtgctt ctagaaggcc tggattagtc cctcctacct
     1921 catgaccatg gatataggca taagcctgtt ctcgaatgta ggctaatagt tctttgtcat
     1981 aaagggtacc aacaaattta atgcgtgggt ctttatcaca ctctgtctca gcaagtaatt
     2041 gcttaaagta agcattgtct tcatgattac aaataatcac taaatcacgc ttagttgagg
     2101 aagccataaa ttctttaata gcagtttcgt aattattttc aggaacaaag cgtcctaaaa
     2161 tgagatagta atccttttct ctaatatcaa aggtttgaaa ataagctctg accttgctat
     2221 ctgctgttgt caaacgtgaa ggttgtgtct gcgttccata agcaataaaa catgttttag
     2281 accaaggata aacctgttta aggtagcggt caatgccaat attatctgaa atgaccaaat
     2341 cagcctgtct agtcatttgc ttttcggaaa acttcaggta ggcctgcact ggttttgacc
     2401 atttagaccg tttccactct aacccgtctg gattgataaa aaagcgtcca cctcgattgt
     2461 gaatctgctt cacaaatggc gcaataaaag cgcctacagt attgcccaac acataaaaaa
     2521 tagggttctg aatctggtgt tggtcgctat aagacagggc ataggtaatt gccatcatat
     2581 cataagcaat aactctagca ggaccaagtt ttgggggatt aaggtaaaag caatctgccc
     2641 ccttataatc aaaatgaacc ttatgcttcg tatcgcttaa acaagcaacg tgataacgaa
     2701 tgtttttaga tgattgatga ctaatcaact cttcgacaaa ggtttcaaag ccaccgtatt
     2761 tcgctggtaa ccctctgcta ccaatgataa aaacatcctg cattgcatac taccccattc
     2821 taaaatataa tcagtgatat tataccacaa ttagaaggct aatgcgacct taaacccatg
     2881 aaaaagagtt ggccttagaa agccaactct ttttagaata cttacttttt cagtccttgt
     2941 tggtaaaatg ccttcaaggc ttcttgccat gttggaataa caaaccctgt tgcttttgcc
     3001 ttatcaagat tcatggttga atttaaagga cgtttggctt ttgctgggaa agctgatgaa
     3061 tcaactggca ccacttcaac agccttgtct tttaagattt ctttggcaaa atcataccaa
     3121 gtggtgtctt ccttagcatc atttgacaag tgatagtaac caaaggcttt ttgattctct
     3181 gtcaaataac acatgaattc tgccaaggtt cttgtccacg ttggccgacc gtgttggtca
     3241 ttgacaacag tcaaacggga gtgattttcc gctaactgct ccatggtaaa gacaaagttt
     3301 ttaccataat ttccaaatac ccaagcagta cggataatat aaaaatgctc tgcgtatcgt
     3361 tcaacagcta gttctcctaa acgttttgta cggccatatt ctgtcttagg gtctggacga
     3421 tctgtttcta cccattcttg accgactggt ttattgccat caaatacgta gtctgttgaa
     3481 atgtaaacaa gggtagcccc atattttcca caagccttgg caatattttc tgagcctgtt
     3541 acattaatag cctcattcaa ggcttttcct tcgtcttctg ctgcatcaac tgccgtataa
     3601 gcagcacaat gatagaccaa tgttggcttg acctgcgcaa aaacagcttc gactttgtct
     3661 tcatttgtga tgtccatttc tgcgacatca acggctacat aatcaacacc tcgttcatct
     3721 aataagtatc gaagttctgt ccctaattga ccattgcttc ctgtaattaa aatcatcagt
     3781 tctccttttt gccataggta gaacactttt ctcagtatct tagtgttctc taacattaac
     3841 caaaggcttt cacctgtatt tccttttatt ctacatcaaa aaagggaata tttcatccct
     3901 ttactatcaa tgattagcga atacccagcg caatcctagc atagcggctc attttatcaa
     3961 ccgtccaagc agggtaccag actaatttaa cctcagtctt agtgacttct ggcacatctt
     4021 gcagggcatc atggatgtaa tctgtcaaca agtctgccaa aggacaaccc attgttgtga
     4081 gtgtcatatc aatctctgta taaccgttat cattgaaccg aatttcataa atcaaaccta
     4141 gattaacaac atcaatacct agctcgggat caataacagt ttctaaagct tcaagaattc
     4201 tattcttgat agctatcacc tgatcttgcg tatattttgg tgtatcagac atggctttcc
     4261 tttcattttt tgaatatgtc gtagataagt aaaaaaacca ctaaagcttt gcctatttag
     4321 aggaattgcc ctctgggctt gtttaagata tcaagtccaa ccttaaaaat acccaccaca
     4381 ttcggtccga tttctaattt gtaggtgtac cgctgataag tgccacttag gattttcgct
     4441 cctttcttat ttcaaaggaa cgacctaaaa tcgtccactg gacgatttta gtcctctata
     4501 aagtctctta attgtttact acgactagga tggcgaagtt ttctaagggc tttagcttca
     4561 atctgtcgaa tgcgctcacg agtaacatta aagactttac ctacgtcctc taacgtacgc
     4621 attttaccat cgtcaaggcc aaagcgtagg cgaagaacgt tctcttcacg gtcagtaagc
     4681 gtatctaaaa cttcatccaa ttgctcacga agaacaacac gcgtcgtata atctactgga
     4741 ttttcaataa cttcatcttc gataaaatca cctaagtgac tatcatcttc ttccccaatt
     4801 ggtgtttcta aagaaacagg ttcttgagca atttttagaa tttcacgaac cttgtctgga
     4861 gtcatttcca tacgttctgc gatttgttct ggcgttggat cttgaccaag ttcttgaaga
     4921 aggttacgtt gttcacggac taatttatta atcgtttcaa ccatatgaac agggatacga
     4981 atcgtacgtg cttgatctgc aattgcgcgt gttatagctt gacgaatcca ccatgttgca
     5041 taagttgaga atttgaatcc ttttgaataa tcaaatttat caacagcttt catgagtccc
     5101 atgttacctt cttgaattaa atcaagaaat tgcattccac gtccaacata acgttttgca
     5161 attgagacaa caagacgaag gtttgcttcc gctaaacgtt gtttagccat taaatctccc
     5221 ttggcaactg caacagccaa ttctttttcc tcttcacttg ttaaaagcgg taccacacca
     5281 atttctttga ggtacatgcg gacagggtcg tttactttag ctgaattact accaattaat
     5341 tcttcatcag ttaattcttc tggttttggt tcttcaacaa tgtattttga tgatgggtta
     5401 ccttctttat ccgtaatcga gataccacca tctgttaacc gctcaagtaa atcatcaatc
     5461 tgatcagcat ccaaagcaaa tggaatgaca agtttttcgg tcacgtcatc atcaatcgct
     5521 gttccttctt ttttgtgatg gcgaataaac tccgcaactt gaacgttaaa agttgttatt
     5581 tctttttgtt ttgtcataat ttcctctatt ccatttttcg tttctgcgca ataaaatgtt
     5641 ctagtacttc tagagccgct tgatgatcgc ctttgttact agattctcta acttttttcc
     5701 cttgtttgtg aagatcgcgc tctgctaaaa gtttggcacg tttggataaa atatcatcaa
     5761 tctcaccaag agctacttct ttgggaaggt tttcttctaa aacattgtaa taagcacggt
     5821 taatttcctc tgacatctct gacaaatcat aagatgtaat gtatccttgt tgcttcagcc
     5881 gttgataaag tatttctaag gtagaggtat caaaataaaa atcatcacga tgtcgaaatt
     5941 catttaataa atagtcatga tgtaagagcc gatgcatgag atgactttct gtcttagcaa
     6001 tagctgtcaa actttttggt attggtaagg tcacaagatt gctgacggct tgagctattt
     6061 gaccttgacg cttttgccta tcttgaatcc ttaatgcatt tactgattgt tctacttgaa
     6121 aatagtcaaa gtttggcaac aaatcagcaa tcttgttaat atacgaatgt tgagctgtga
     6181 tggatggtga ttgagcaatc aatggtgcca ttttctccac ataaacaatt tgtgattgca
     6241 gattgtctac attagtaggt tttaggtaat caataaaaaa ttcaacacta ctgatccgtg
     6301 actgcttaag caaatctgca aatgcttctg gggaatgccg ttgtacaaat tcgtcaggat
     6361 ccattttatt ggggattctg acaatttcga caacaaaatc tttaagcaat tctagtgatt
     6421 ttgcaatagc atgttgtcca gcatcgtcac catcataaat caaaacaact tttttagtga
     6481 cttgcttaag gtgattgaca tgttcttgag tcaatgccgt ccccattgaa gcaacagcat
     6541 tttcatagcc ggaacggtaa gcggcaatca cgtccataaa cccttccatt agaaacactt
     6601 catgggtttt ggcaataaca ggccttgctt tgtccagatg atacaattca taagatttgt
     6661 taaaaagaac tgttcctctt gaatttttat acttggcctg tctcttttcc atatcagctg
     6721 ccgtccagat acgtcctgaa aaggcaataa tatgccctcg gtcatctgat aagggaaaca
     6781 tgatacgatt tcgaaaggcg tcgtaaatgg tattggattg atcggacaag ttaaacaatc
     6841 ctgaagcaac caattgacct tcctcgtatt ttttagaaag agcttgataa agataatctg
     6901 actcatctgg ggctaaacca atattgaaat gctcaattaa ttggtcatcc aagcctctct
     6961 ggtaaaggta cttcctagct tcttgaccaa tggtagtggt catcaaaact gcatggtaaa
     7021 atttagcagc atcctcatga agtgtcatca aagcgtgatt agggtgcttg tgttggctag
     7081 caagtactgc ctgacttggc ggtatattaa gcgacatacc agtcttatcg gcaataatct
     7141 gaacactttc taagaagggg acttggcggt attcctcaat aaatttaaaa acatcccctg
     7201 attttccaca gccaaagcag tgaaaaaatt gtctgtcttc aacaacattg aaagagggtg
     7261 tcttttcctt atgaaatggg caaagcccga gataatgccg ccctgatcgg gaaagtttga
     7321 ccacttctcc aatgacatcg acaatattaa cgctattttt tacttgggaa atcatttctt
     7381 tgtcaattgc caaatcgtca cctccccata aaaatcccat tttagtttta cgttttatta
     7441 taccaccgaa actctttttt gtaaatttaa aaactgaagt gattccattg gattttgttt
     7501 actaattatg ctatgcttaa cttagagata ttaaaagggg gaatatttaa tggttaaaga
     7561 attaaaagca tttttgttta gaggaaacat tattgaactt gctgtagcag ttatcatcgg
     7621 gggagctttt ggtgctattg tcacttcttt tgtgaacgat attatcacac cgcttatttt
     7681 aaacccagct ctgaaggctg caaatgttga aaacatcact caattgtcat ggaatggtgt
     7741 taaatatggt agctttttag gagccgttat taattttctt atcatcggta ccagtctttt
     7801 ctttgttgta aaagccgctg aaaaagctat gcctaaaaaa gaaaaagaag ctgcagctcc
     7861 tactcaagaa gaattattga cagaaatccg tgatttgttg gctcaaaaat aaagcgattt
     7921 ttgataaagt agttaggcat gtctaactgc tttttttgat atcaaaaaag ctcccttgct
     7981 tcttctggat aaaaaataaa aacagttcta attgtgagaa ctgtttttat tttttaccat
     8041 tagaattttt tacgtttgcg agctgcttct gatttacgtt tacgttttac agaaggtttt
     8101 tcgtagaatt cacgtttacg tgattcttga agagtaccag ctttagtaac agaacgtttg
     8161 aagcgacgaa gagcatcgtc aagagattca ttcttacgta ctactgtttt tgacatgtgt
     8221 ttcacctcct caagatattg taacattttc acgttcccaa ttatattaca tta
//
LOCUS       DAUGHY010000055         7593 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.055, whole genome shotgun sequence.
ACCESSION   DAUGHY010000055 DAUGHY010000000
VERSION     DAUGHY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.055"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            327..755
                     /gene="lacA"
                     /locus_tag="VH752_001449"
     CDS             327..755
                     /gene="lacA"
                     /locus_tag="VH752_001449"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ8289791.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKYEVVDVSENEVRDFVDTT
                     LAVAKEVNASEDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNARII
                     TLGSEISAPGIAKNIIKGFVEGKYDGGRHQVRVDMLNKMC"
     gene            790..1305
                     /gene="lacB"
                     /locus_tag="VH752_001450"
     CDS             790..1305
                     /gene="lacB"
                     /locus_tag="VH752_001450"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEQ8289792.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKSQGHKVIDCGTYDNVRTHYPI
                     FGKKVGEAVASGEAELGVVICGTGVGITNAVNKVPGIRSALVRDMTSAIYSKEELNAN
                     IIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWDRGEYHD"
     gene            1351..2280
                     /locus_tag="VH752_001451"
     CDS             1351..2280
                     /locus_tag="VH752_001451"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEQ8289793.1"
                     /translation="MILTVTLNPAIDVSYPLDELKCDTVNRVVDVTKTPGGKGLNVSR
                     VLNDFGETVKATGCVGGESGDFIINHLPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPLLSVDEIDGFTHQFKYLLNDVDVVTMSGSLPAGMPDDYYQKLIKIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTKDFEALKEVLQDELFEGIEWI
                     IVSLGADGVFAKHKDTFYNVDIPKIKIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKVKEV"
     gene            2284..3267
                     /gene="lacD"
                     /locus_tag="VH752_001452"
     CDS             2284..3267
                     /gene="lacD"
                     /locus_tag="VH752_001452"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ8289794.1"
                     /translation="MTITLTENKRKSMEKLSIDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEVGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDINEQKKAYIERIGSECRAEDIP
                     FYLEILTYDEKISDNASPEFAKVKAHKVNEAMKVFSEERFGVDVLKVEVPVNMKFVES
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLVFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            3291..3608
                     /locus_tag="VH752_001453"
     CDS             3291..3608
                     /locus_tag="VH752_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ8289795.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            3608..5305
                     /locus_tag="VH752_001454"
     CDS             3608..5305
                     /locus_tag="VH752_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ8289796.1"
                     /translation="MNGLIAQIEKGKPFFEKMSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIESLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFMSTMLAAIVGFLLMAANPAKEGGFLTGFMGTKGLLTAFIAAFITVNVYKTCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHSTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLIVVDVIIYYPFLKIYDEQILAEEAAGTNSSDALKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKEQMEK"
     gene            5378..6784
                     /gene="lacG"
                     /locus_tag="VH752_001455"
     CDS             5378..6784
                     /gene="lacG"
                     /locus_tag="VH752_001455"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEQ8289797.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGEVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVNYAELCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDSGYSGEIGVVHAL
                     PTKYPFDANNPDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFAALDAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFMWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            7287..7442
                     /locus_tag="VH752_001456"
     CDS             7287..7442
                     /locus_tag="VH752_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A2 lantipeptide"
                     /protein_id="HEQ8289798.1"
                     /translation="MSFMKNSKDILTNAIEEVSEKELMEVAGGKKGSGWFATITDDCP
                     NSVFVCC"
BASE COUNT         2510 a         1201 c         1541 g         2341 t
ORIGIN      
        1 aaacgtttta aataaaacgt ttacaataaa tagagataag ggtctcgtaa tatatgatag
       61 gttacgagtg gctgtcaaag ggatttatga aatgaaaaag aaacacaaga aatattagga
      121 taataacgta aaaatgaaaa aaataaacat attttgttta ttttttataa aaataaacaa
      181 aaaaatgttg acttttatgt ttggtgggtg tatgattagg tcatagaaat aatgaaagcg
      241 ttatttcaaa agatgttgca aatcgttaat taactatcta cgtaggtttt ccacatttac
      301 ttattctaaa tcataggaga aataaaatgg caattattat cggtgctgac aaagcaggtc
      361 aagaacttaa agaagttatc aaagattacc ttaaagaagg taaatacgaa gtcgttgatg
      421 ttagtgaaaa cgaagtacgt gactttgtag atacaacttt agccgttgca aaagaagtta
      481 atgcctccga agataactta ggtattgtca ttgatgcata tggtgttggt tctttcatgg
      541 tagctactaa aatcaaagga atggtggcag ctgaagtatc agatgagcgc tcagcataca
      601 tgacaagagg acacaacaat gctcgaatca tcacgcttgg ttctgaaatt agtgctccag
      661 gaattgcaaa gaacattatc aaaggctttg tcgaaggtaa atatgatggt ggacgtcatc
      721 aagttcgtgt cgacatgctt aataaaatgt gctaattgat tgaaataggt taaaacaagg
      781 agaacaaaaa tgaaaattgc tgtaggttgt gaccatattg ttacttatga aaaaatcgct
      841 gttgtggatt acctaaaatc acaaggtcat aaagttattg attgtggtac gtatgataat
      901 gttcgcacac actatccaat ttttggtaaa aaagttggtg aagcagtagc aagtggtgaa
      961 gcagaactag gtgttgttat ctgtggtact ggtgttggta ttacaaatgc cgtaaacaaa
     1021 gttcctggaa ttcgttcagc actcgttaga gatatgacat ctgctatcta ctcaaaagaa
     1081 gagctaaacg caaacattat tggttttggt ggaaaaatca tcggtggttt attgatgaat
     1141 gatattatcg atgctttctt agccgcggaa tacaaaccaa ctgaagaaaa taaaaaatgg
     1201 attgaaaaaa tggatagctt gcaacatgct agccaagatc aaaacaaccc acacttcttt
     1261 gatgaatttc tagaaaaatg ggaccgtggt gaatatcacg actaatattt gaaagaatta
     1321 tgataaggat atatagaaaa aggagctatt gtgattttaa cagttacttt aaatccagct
     1381 attgacgtct cctacccttt ggatgagcta aaatgcgata ctgtcaatcg agttgtagac
     1441 gttacaaaga cacccggtgg taaaggcttg aatgtttccc gagttctaaa tgactttggt
     1501 gagacagtca aagctacagg ttgtgtaggt ggagaaagcg gtgattttat cattaatcat
     1561 cttcctgatt cgattttaag tcgcttctat aaaatttcag gtgatactag aacttgtata
     1621 gccattttac acgaaggtaa tcaaacggag attttagaaa aaggaccgct gttaagtgta
     1681 gatgaaattg atggatttac tcatcaattt aagtatttac tgaatgatgt tgacgtagtg
     1741 acaatgtcag gcagtttgcc tgctggtatg ccagatgatt actatcagaa gttaatcaag
     1801 attgccaatc ttaatggtaa gaaaacggtt ctggattgtt ctggaaatgc tctagaagca
     1861 gtattgaaag gtgatagtaa accaacagtt atcaagccta atcttgaaga actttctcaa
     1921 cttttaggta aagaaatgac aaaagatttt gaagccttaa aagaagtctt acaagatgaa
     1981 ctatttgaag gaattgaatg gataatcgtt tctcttggtg ctgatggtgt atttgcaaag
     2041 cacaaggata cattttataa cgttgacatt cctaaaatca aaattgtaag cgctgtaggt
     2101 tcaggggatt ctacagtggc aggtattgct tcaggtttag cgaatgatga agatgatcga
     2161 gctttattaa caaaagcaaa tgtcttaggg atgttaaatg cgcaagaaaa aacaactggg
     2221 catgttaaca tggcaaatta cgataagtta tatcaatcta ttaaagtcaa agaggtataa
     2281 aaaatgacaa ttactttaac tgaaaacaaa cgtaaaagca tggaaaaact aagtatagat
     2341 ggtgttattt ctgctttagc ttttgaccaa cgtggagctc tcaaacgtat gatggctcaa
     2401 catcaaacaa aagagccaac agtagaacaa attgaagagc ttaaaagttt agtttctgaa
     2461 gaactaacac catttgcatc atcaatttta cttgatcctg aatatggctt gccagcaagt
     2521 cgtgttcgtt cagaagaagt aggcttgttg ttggcatatg agaaaacagg atacgatgca
     2581 acaacaacta gtcgcttacc tgattgctta gatgtttggt ctgcaaaacg tattaaagaa
     2641 gctggagcag aagcagttaa attccttctt tactatgata ttgatggaga tcaagatatc
     2701 aacgagcaga aaaaagctta cattgaacgt attggttctg aatgccgtgc agaagatatt
     2761 ccattctact tagaaattct aacttatgat gagaaaatct ctgacaatgc tagccctgag
     2821 tttgcaaaag ttaaagctca taaagttaat gaagcaatga aagtcttctc agaagaacgc
     2881 tttggtgttg acgtcttgaa agtcgaagtt ccagtaaata tgaaatttgt agaaagcttt
     2941 gcagatggtg aggttctctt tacaaaagaa gaagcagctc aagctttccg tgatcaagaa
     3001 gcttctactg atttaccata tatttacttg agtgcaggtg tttctgccaa attgttccaa
     3061 gatactttgg tgtttgcagc agaatcaggt gctaaattca acggtgtctt atgtggacgt
     3121 gctacctggg caggatctgt aaaagtctat atcgaagaag gaccacaagc agctcgtgaa
     3181 tggttgcgta cagaaggttt caaaaatatt gacgaattaa acaaagtgct agataaaact
     3241 gcaagtcctt ggactgaaaa aatgtaacat tattttcgga ggaaactaca atgaatagag
     3301 aagaaactac tcttttaggt tttgaaatcg tagcctatgc tggtgatgcc cgttcaaaat
     3361 ttttggaagc gcttaaagca gcgcaagaag gagattatgc taaagcagaa gaattaatcg
     3421 ctgcaggtag tgactgcttg aatgatgctc acaatgctca gacaagtcta ctacaaaaag
     3481 aagcagctgg agatgactta gcttatagtg tcactttaat gcacggtcaa gatcatttaa
     3541 tgacgaccat tttattacaa gatttaatga aacacatgat tgaactttat aagagaggag
     3601 caaaataatg aatggattaa ttgctcagat tgagaaaggg aaacctttct tcgagaaaat
     3661 gtctcgaaat atctatcttc gcgctattcg tgatggtttt atcgctggta tgccagttat
     3721 cttgttttca agtatcttta tcttgattgc ctatgttcca aatgcatggg gcttccattg
     3781 gagtaaggat attgagagtc tcttgatgac gccatacaac tactcaatgg gtattttagg
     3841 tttgtttgtg gctggtacga cagccaaagc tttaactgac tcaatgaatc gatcattacc
     3901 aagcacaaac caaatcaact tcatgtctac catgctcgct gctattgtag gttttttact
     3961 tatggcagcc aatccagcaa aagaaggtgg tttcttaact ggatttatgg gaacaaaagg
     4021 tttgttgaca gccttcatcg cagcttttat taccgtaaat gtctataaaa cctgtgtgaa
     4081 aaacaatgtc actattcgca tgccagaaga ggtgccacca aacatctctc aagtttttaa
     4141 agatttgatt ccatttacgt tatcagttgt actgctttat gtgattgaat tgattgttca
     4201 tagtacttta ggcgttaacg ttgccgagtc aattggtaaa cttcttgcac cattgttcca
     4261 agcagcagat ggctatgttg gaatcactat tatcttcggt gcatttgctt tcttctggtt
     4321 tgttggtatt catggaccat ctattgttga accagccatt gcagcgatta cttacgcaaa
     4381 tgctgaaacc aaccttcaat tacttcaagc tggtgaacat gctgacaaga ttttgacttc
     4441 agggactcaa atgttcatcg ttaccatggg tggtactggt gcaactctcg tcgttccatt
     4501 catgttcatg tggttaacaa aatcaaaacg taacaaagcc attggacgtg catcagttgt
     4561 tccaacattc tttggtgtta acgaacccat tttgtttggt gctccacttg ttttgaaccc
     4621 aatcttcttt attccattta tcttggcacc aattgtcaat gtatggattt tcaaattctt
     4681 cattgacaca cttggaatga acagtttcac tgcaaactta ccttggacaa caccagctcc
     4741 attgggtctt atccttggta caaacttcca agtgctagca ttcattttgg cagctttgtt
     4801 aatcgtagtt gatgtcatca tctactaccc attcttgaaa atttatgatg agcaaattct
     4861 tgctgaagaa gcagcaggta cgaattcatc agatgccctc aaagaaaaag tagcagcaaa
     4921 ctttgatact aaaaaagctg atgctattct tgaaaaatca gcagctaaag aaaccaaaga
     4981 aatcactgat caaactaatg ttctcgttct ttgtgccggt ggtggtacaa gtggtttgct
     5041 tgcaaacgct ttgaataaag cagctaaaga atatggtgca cctgtcactg cagcagcagg
     5101 aagttatggt gctcaccgtg agattttacc acaatatcaa cttgttatcc ttgcacctca
     5161 agtagcttct aactatgaag atatgaaagt ggaaactgat aaacttgata ttaaattagc
     5221 taaaacagaa ggggctcaat acatcaaatt gactcgcgat ggtcaaggcg cattagactt
     5281 cgttaaagaa caaatggaaa aataatatgt cgctagggaa ggttgggggt actcaatctt
     5341 cctttatagt gaaatgaaaa tttagaagag gtatatcatg actaaaacat tacctaaaga
     5401 ttttattttt ggtggtgcta cagctgctta ccaggctgaa ggcgctaccc acacagatgg
     5461 taaaggacca gtagcttggg ataaatactt agaagacaac tattggtaca cagctgagcc
     5521 agcaagtgat ttttataatc gttaccctgt cgatttgaaa cttagtgaag aatttggtgt
     5581 taatgggatc cgtatctcta ttgcctggtc tcgtattttt ccaacaggaa aaggagaagt
     5641 taaccctaaa ggagtagaat actaccacaa tctttttgca gagtgtcata agcgtcatgt
     5701 tgagcctttt gttacacttc accattttga tacaccagaa gcacttcatt caaatggtga
     5761 tttcctcaat cgtgagaaca ttgaacattt tgtaaattat gcagaactct gttttaaaga
     5821 attctcagaa gttaactatt ggacaacctt caacgaaatt ggaccaattg gcgatggtca
     5881 atacttggtt ggtaaattcc ctccaggtat ccaatatgat cttgctaaag ttttccaatc
     5941 acatcataac atgatggtct ctcatgctcg tgcagtcaaa ctctttaaag atagtggtta
     6001 ttcaggtgaa attggtgttg tccatgctct tccaactaag tatccatttg acgctaacaa
     6061 tcctgatgat gttagagcag ctgaactgga agatatcatc cataataaat ttattcttga
     6121 tgcaacttat ctaggtaagt attcagataa aacaatggaa ggtgttaacc atatccttga
     6181 ggtgaatggt ggtgaacttg atcttcgtga agaagatttt gctgcactag acgctgcaaa
     6241 agatttgaat gacttccttg gtattaacta ctatatgagt gattggatgc aagcttttga
     6301 tggtgagact gaaatcattc acaatggtaa gggtgaaaaa ggcagctcta aataccaaat
     6361 caagggtgtt ggtcgaagaa aagcacccgt tgatgttcca aaaacggact gggactggat
     6421 tatcttccca caaggcttat atgatcaaat catgcgtgtc aaagccgatt atcctaacta
     6481 caagaaaatt tacattacag agaatggtct tggctacaaa gatgagtttg tagataatac
     6541 tgtctatgat gatggacgta tcgattatgt gaaaaaacac ttagaagtta tttctgatgc
     6601 tatttctgat ggtgcaaatg ttaaaggata ctttatgtgg tcactgatgg atgtcttttc
     6661 atggtcaaat ggctatgaaa aacgttacgg tctcttctat gtcgattttg aaactcaaga
     6721 acgttatcct aagaagagtg cctactggta taaaaaagta gcagaaactc aagtgattga
     6781 atgattttga gatacttttt taatacattt ttacacttca tgttcgttta ctgaattata
     6841 gtcacaaaaa gtagatgatg ttgacatttt tcaagcatag tattatttgc tgttgatggt
     6901 aatccattag ttgtaagatc gggttataag tgtttttagt tgatgaaaat gggttttgaa
     6961 agttttattt aacatccttt ttcagatatt tgacttctgt cattcaaact cctgacctga
     7021 ttaaataagt aaagtaacca ttttctgatt agagattgat gttataacga gaagcgatta
     7081 gattgtaaaa tagctgtaaa gaacgagttt tgttttttat atcagagggc tatttgttat
     7141 aaagtgacct cttatgtgac atctcaaaaa tcttactaaa aaatatgaaa aaagtcattt
     7201 ttgcactttt tactcattga aaatgaagac gtattattta caataaaagt gtagaaaata
     7261 gtcactacat actgatagga aggagaatga gttttatgaa aaattcaaag gatattttga
     7321 ctaatgctat cgaagaagtt tctgaaaaag aacttatgga agtagctggt ggtaaaaaag
     7381 gttcaggttg gtttgcaact attactgatg actgtccgaa ctcagtattt gtttgttgtt
     7441 aattaaaaca atctggataa gcagactaaa atttcctgtt tagacttagt ctaggcagga
     7501 tttttaagta gtggtaggaa aaaatgataa aaagaaatga attaaagtta gagtatcagc
     7561 aacatcagtt ttacgaatac tttaactcaa ttt
//
LOCUS       DAUGHY010000056         7107 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.056, whole genome shotgun sequence.
ACCESSION   DAUGHY010000056 DAUGHY010000000
VERSION     DAUGHY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.056"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            68..1291
                     /gene="pepT"
                     /locus_tag="VH752_001457"
     CDS             68..1291
                     /gene="pepT"
                     /locus_tag="VH752_001457"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEQ8289799.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPE
                     MEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALELAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAHLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            1332..1820
                     /locus_tag="VH752_001458"
     CDS             1332..1820
                     /locus_tag="VH752_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEQ8289800.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGLHRYFIIENNGILRIVSFKLFGPRKLLISTITKIEVTKSTL
                     CLHVEDKSYLFYMRKWPKKYFLDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALT
                     RG"
     gene            complement(1807..2004)
                     /locus_tag="VH752_001459"
     CDS             complement(1807..2004)
                     /locus_tag="VH752_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEQ8289801.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            2053..2529
                     /locus_tag="VH752_001460"
     CDS             2053..2529
                     /locus_tag="VH752_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ8289802.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTRKASNAKKTSSSSTTTDTTPSSEETL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            2544..3224
                     /locus_tag="VH752_001461"
     CDS             2544..3224
                     /locus_tag="VH752_001461"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEQ8289803.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPIFFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWI
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKAAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    3248..3371
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            3386..3916
                     /gene="infC"
                     /locus_tag="VH752_001462"
     CDS             3386..3916
                     /gene="infC"
                     /locus_tag="VH752_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEQ8289804.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDKK"
     gene            3958..4155
                     /gene="rpmI"
                     /locus_tag="VH752_001463"
     CDS             3958..4155
                     /gene="rpmI"
                     /locus_tag="VH752_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEQ8289805.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            4214..4573
                     /gene="rplT"
                     /locus_tag="VH752_001464"
     CDS             4214..4573
                     /gene="rplT"
                     /locus_tag="VH752_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEQ8289806.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVAD
                     AAAFTALADAAKAKLGK"
     gene            complement(4864..7074)
                     /locus_tag="VH752_001465"
     CDS             complement(4864..7074)
                     /locus_tag="VH752_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEQ8289807.1"
                     /translation="MTSLGKNDKEVATVKKFKTLITGFINTRLGFIITLLFCYWIKTL
                     WAYHTDFSLDLGNIYQVFLTIINPIPLAFLLLGGALYVKNTRAFYICSWVVYIILNIL
                     LISNSIYYREFSDFITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSIT
                     KKIKNDQRPFNKRAAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPA
                     FTLYSGNQTYQAQKERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLES
                     FQQFLIDYKLKEGDKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSL
                     FGLNSGSFMVNYGGENTQFATPSILTQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYF
                     FDSSYFSKQNSKNSFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGE
                     SSEEGFPLAKTDDETINGYFATANYLDAALKSFFDYLKATGLYDNSIFVLYGDHYGIS
                     NSRNSSLAPLLGKDSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLL
                     HILGIDTSQFVQLGQDLLSPQNSQIVAQRTSGTYMTPEYTNYNGRLYNTQTGLEITNP
                     DEMTIAKTKEIRSAVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKD
                     ISAKLGSESTSLYSKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNTKNRE"
BASE COUNT         2331 a         1168 c         1416 g         2192 t
ORIGIN      
        1 ttagcttgaa taagtatttt aaagcctttt atgctataat taaattgaga aattattgag
       61 gtggcctatg aagtacgata acttgttaga tcgatttata aaatatgtca aagtgaacac
      121 aagaagcgat ccagacagtg agactactcc tagtacggag agtcaagaag catttgcctt
      181 aacgatttta aagcctgaaa tggaggcaat tggtttgcaa gatgttcatt ataatccagt
      241 taatggctat cttattggaa ctctgcctgc caataatcca accttgacgc gtaaaattgg
      301 ttttatagcc catatggata cggctgattt caatgctgaa aatgttaatc cgcagattat
      361 tgacaattat caaggagggg acattacact tggcagttct aattataagt tggatccaaa
      421 agcatttcca aatcttaata attatatcgg tcaaacattg attaccactg atgggactac
      481 acttttaggg gcagatgata aatctggtat tgctgaaatc atgacagcaa tagaattctt
      541 aacatcacaa ccacaaatag agcactgtga cattaaagtg gcttttggcc ctgatgaaga
      601 gattggggtc ggagctgata agtttgaggt ggctgatttt gaggtggatt ttgcttatac
      661 tatggatggc ggtcctttag gcgaattgca atatgaaact ttcagcgctg cagctctaga
      721 agtgactttt ttaggcagaa atgttcatcc tggtacggct aaggatcaaa tgattaatgc
      781 ccttgagtta gcaatagatt ttcatgagaa gctacctgct aaagagcgac ctgaatacac
      841 agatggttat caaggcttct atcacttaac gggactaact ggaacagttg aagaggcacg
      901 cgcatcttac attattcgtg attttgaaga agcatcattt gaagctagaa aagtgaaagt
      961 tgaaaatata gctcaatcga tgaatgctca tctaggaaca aaacgtgtct tggtagagct
     1021 taatgaccaa tactacaata tgaaaaaagt tatcgaaaaa gatatgaccg ctattgagtt
     1081 agctaaagaa gtaatggaag agcttgctat caagcctgta atcgaaccca tacgtggagg
     1141 aacagatggc tctaaaattt catttatggg tattcccact cctaatattt ttgcaggggg
     1201 tgagaatatg cacgggcgtt ttgagtttgt tagcttacaa acgatggaga gagctgtcga
     1261 tgtcattatt ggacttgttt gcaaagccta attatcatgt aattgatatt tagtggaagg
     1321 ggcttataaa aatgattaaa ctatttggta aaataaggta tcactggcag ccagagttgt
     1381 cttggtctat tatttattgg tctattgcgt ttgcacctat ttttgtaggt ttatctttgt
     1441 tgtatgagcg cactgaaata cctagtcggg tttttattct atttgctatt tttgctgtcc
     1501 tagttggcat tggattacat cgttatttca ttattgaaaa taatggtatt ttaagaatcg
     1561 tatcttttaa attgtttggt ccacgtaagc tacttatttc tactattact aagattgagg
     1621 tgaccaaatc aacactttgt ctgcacgttg aagataaaag ttatctcttt tatatgcgca
     1681 aatggcctaa aaaatatttt cttgatgcct tagcggttaa tccatacttc caaggagaag
     1741 ttatattaag tgataacttt attaaattag actattttga agtttatcag catgataaaa
     1801 aagcccttac tcgagggtaa gagctttagt tgggcaagat ttgacagcta aaatagcgtc
     1861 cttatctgaa ggacaaattg actgacttgt ttcagatgag cttgaaaatg tgacgatacc
     1921 attgtcatga taatcaaaaa gtgatgaata ggtttggcat agaccacaag cgatgcattt
     1981 ttctggaatg atagatactt tcataactat tattctaata tgattttgtt aaattaacaa
     2041 ggggggacga tcatggctaa agaaccatgg gaagaaaaaa ttgttgatga tactataggg
     2101 acacgaacac gtaaatcaag aaatgctttc attagcacgc cttggttgac tgctttatta
     2161 agtgtattct ttgtcatcat tgttgctata ctttttattt tcttctatac atcaaatagc
     2221 ggtagtaata gacaagctga aacaaatggt ttttatggag catccactca taaaaaaaca
     2281 aggaaagctt ctaacgctaa aaaaacatca agtagttcaa caactacaga cacaacacct
     2341 tctagcgaag aaacacttgc ttctagtgaa ggaactggcg aaacccttac tgtattggca
     2401 ggtgaggggg cagcttctat tgcagctcgt gcaggtattt ctgtggagca gctacaagca
     2461 cttaatccag agcacatgac tcaaggatat tggtatgcca atccaggaga tcaagtcact
     2521 attaaataat tgaagaggaa aaaatgaaag caattaaaat tgcgattgat gggccagctt
     2581 caagtggaaa aagtacagta gccaagatta ttgcaaaaaa tcttggctat acttatctag
     2641 atactggtgc tatgtatcgc tcagcaactt atatcgcact gacacatggc tatacgggta
     2701 aagaagtagc ccttatttta gaggaacttg agaaaaatcc tattttcttt aaaaaagcaa
     2761 aagatggctc ccaattagtt tttcttggag atgaggacgt gaccttagcc attaggcaaa
     2821 atgatgtgac taacaatgtt tcttggatct cagctttgcc agaaattaga gaagagctgg
     2881 ttcatcaaca aagacgcatt gcacaagctg gtggcattat catggatggt cgtgacattg
     2941 gaacggttgt tctcccagat gcggaactaa aaatcttttt agtggcttct gtcgaagagc
     3001 gggcagagcg ccgctataaa gaaaacctag aaaaaggaat tgaatcagat tttgagacgc
     3061 taaaagaaga gattgcagct cgtgattaca aggatagtca tcgtaaggtt tcacctctaa
     3121 aagcagctga agatgccctt atttttgata ccacaggtgt ttctattgat ggcgtggtgc
     3181 aatttatcca agaaaaagca gaaaaaatag ttgacatgtc ctagagccta tgttagaata
     3241 ttaacaatga gaaaagtaga agtgagagct tctcgccttg cgacttaggt tgtctggccc
     3301 taacatgtcg aattcctatg gaatattatg tgtgcgggct tgatttatca agtccgcttt
     3361 cgtttttctc taaaaaaata aagaggtgaa gatcatagct aaaaaggatc tattcattaa
     3421 tgatgaaatt cgcgttcgcg aagttcgtct cgttggtctt gaaggtgaac aattaggtat
     3481 taaaccattg tctgaagcgc aatcattggc tgatgcttca aatgttgatt tggttttaat
     3541 ccagccacaa gctgttcctc ctgttgccaa acttatggac tatggaaagt tcaaatttga
     3601 gtatcaaaag aaacagaaag aacaacgtaa aaaacaaagc gttgtgactg tgaaagaagt
     3661 tcgtctcagt ccagttatcg ataaaggtga ctttgaaaca aaacttcgta acggtcgtaa
     3721 attccttgaa aaaggaaata aagttaaggt ttctattcgt ttcaaaggac gtatgattac
     3781 tcataaagag attggtgcca aggtactagc tgactttgct gaagcgactc aagatattgc
     3841 tatcattgag caacgagcaa aaatggatgg acgccaaatg tttatgcaac ttgcaccaat
     3901 ttcagacaag aagtaattgt cacagtttca ttatatcttt aagaggagaa tactaaaatg
     3961 ccaaaacaaa aaacacaccg cgcatcagct aaacgtttta aacgtacagg ttcaggcggt
     4021 ttgaaacgct tccgcgcctt tacatcacac cgtttccacg gaaaaactaa aaaacaacgt
     4081 cgtcatcttc gtaaagctgg tttggttagc tcaggagatt tcaaacgtat caaagcaatg
     4141 gttactggtc tttaatttta aaggcagtag acatttacga gtattataag aattatttga
     4201 aggagaacta ttaatggcac gtgttaaagg tggcgttgtt tcacgcaaac gtcgtaaacg
     4261 tattttaaaa ttagctaaag gttactatgg agcaaaacac atcttgttcc gtactgcaaa
     4321 agagcaagtg atgaactctt actactatgc ataccgtgac cgccgtcaga aaaaacgtga
     4381 tttccgtaaa ttatggatca ctcgtatcaa tgcggctgct cgtatgaacg gtttgtcata
     4441 ctcacaattg atgcacggtt tgaaacttgc tgaaatcgaa gtaaaccgta aaatgcttgc
     4501 tgatttagca gtagctgatg cagcagcttt cacagctctt gcagatgcag ccaaagcaaa
     4561 acttggcaaa taagaaacgc tatacctaga aaataacttt ttattaccta tagaaagtta
     4621 taaagaaaca aaaatgaagg agacgatgga cgtcttcttt ttattatact caatataata
     4681 aaaagaagtt tcccatgttt tattaccagt aatgtgggat atttagatgg tagcaagaag
     4741 ttttatagtt gatttgtttt ctttaggtct aattagcata ttttgattac tgataaactt
     4801 gaatatcgcc aataaaaaag aagcaaaatt attattttgc ttctagtcag atcattaaat
     4861 tgattattct ctatttttgg tgttatcctc ttttagttca gaagttgccg cgtcagcttc
     4921 tacgggattt agttctaagt aagatggtgc tttaaaaagt ttctgagttg atttatgacc
     4981 atttttactg tataaacttg ttgactctga tccgagtttt gctgaaatat ctttcagttg
     5041 ttttaactgc ttagtgtaga taaactggtt aggatcaata gcttttagac cattttgagt
     5101 atcaaagcgc aggagatcac ccgtttgaat agcgtcgcta gctgctagtt gttgagcaac
     5161 agcagagcga atttccttag ttttggctat agtcatttca tcgggattag tgatttctaa
     5221 acctgtttgc gtgttgtaca gacgtccatt atagttagtg tattcaggag tcatataagt
     5281 cccagatgtt cgttgtgcca caatctggct attttgaggt gataataaat cttgtcctaa
     5341 ttgaacaaac tgactagtgt caataccaag tatgtggagt aaagtaggaa gagcatcgat
     5401 ttcaccgcca aaggtttctt tgatactacc attcgtataa cctggaatat ggatcatata
     5461 cgggactcgt tgtagcatag cattatcata ttcagaccaa gtttcagaat ctttaccaag
     5521 aagtggagca agactagaat tacgagaatt tgaaatacca taatggtcac catataaaac
     5581 aaaaatagaa ttgtcgtaca aaccagtggc tttcaagtaa tcaaaaaaag atttaagggc
     5641 agcgtctaaa taattggcag tagcaaagta accattgatc gtttcatcgt ctgttttggc
     5701 taaaggaaaa ccttcttcac tactttcgcc ttttagacta gtataaggat agtgattact
     5761 cacagtgata aacttggtat aaaaaggttg ttgcatctgt tcaagatatt ttatggaatc
     5821 cttaaacatg tacttatcat ttaacccata ttgaaaagag ttcttactat tttgtttaga
     5881 gaagtagcta gaatcaaaaa aataattata gccccattgt ttataagcat tattgcgatt
     5941 ccagaaagtt ccaacgttac catgaaagac agcgctggta tagccacctt tttgggttaa
     6001 aatacttgga gtagcaaatt gtgtattttc accaccgtag ttcaccataa aagaaccact
     6061 atttaaacca aataaggaat tctccatcat ggtttcagca tcagaagttt tacctgcttt
     6121 aacctgatga aaaaagttag ggaatgctaa ggtagcattt gagtgataaa gcgagttgat
     6181 aaaaggcgtt acctcatatt ctttatctcc ttcttttagt ttataatcta ttaagaattg
     6241 ttgaaagctt tctaaatgaa gaacaataat atttttgcct tttccaattc caaaatactg
     6301 aggatcaggt gctgcataat gtcctttgac ataagtttta acatcaatta attcttctgc
     6361 agtagctcca tttcgttctt tttgagcttg ataggtttga ttgccgctat agagggtaaa
     6421 cgctggtagt cctaaagctc tcacaatata agtatttgaa aagccacgtg ttaatagttc
     6481 cggacgatca atttctgcta aaaaaagatt aatagaaagc aatagactag ataaagcggt
     6541 gatggcaaat gctgcgcgct tgttaaaagg acgttggtca tttttgattt ttttagtgat
     6601 agataaacta attagaataa taaaatcaag aatatagata atatcccaga tgcgtaaaag
     6661 gtttaaagca gagtccccta atccagcgga gactttacta ctagctagca tggcactcac
     6721 tgtaataaag tctgaaaatt ctcggtaata tatagaattt gatatcaaga gaatgttaag
     6781 gataatataa accacccaag aacagatata aaaggctcgg gtattcttaa cgtaaagagc
     6841 accccccaaa agtaaaaaag ctagtgggat tggattgata atagttagaa agacttggta
     6901 tatatttccc aaatctagtg aaaagtctgt atgatatgcc cacagggtct ttatccagta
     6961 acaaaagagt agagtaatta taaaacccaa tcttgtgttg ataaaacctg tgataagtgt
     7021 tttaaatttc ttcacagtag caacttcctt atcgtttttt cctaaagatg tcatctccaa
     7081 ataaaatctc agattaaatt ataccaa
//
LOCUS       DAUGHY010000057         7052 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.057, whole genome shotgun sequence.
ACCESSION   DAUGHY010000057 DAUGHY010000000
VERSION     DAUGHY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.057"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(134..1393)
                     /locus_tag="VH752_001466"
     CDS             complement(134..1393)
                     /locus_tag="VH752_001466"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ8289808.1"
                     /translation="MRKIIINGGKALSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLSTNGQKIHGAHIYM
                     DTVSVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVTAGLIAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
     gene            complement(1567..2763)
                     /locus_tag="VH752_001467"
     CDS             complement(1567..2763)
                     /locus_tag="VH752_001467"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEQ8289809.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTTAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEALESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKV
                     IKAVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDATKVDRSASYAARYIAKNLVAAGLATKAEVQLAYAIGVAQPVSVRVDTFGTSTVPE
                     AVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVE
                     AVKTVL"
     gene            complement(3299..5677)
                     /locus_tag="VH752_001468"
     CDS             complement(3299..5677)
                     /locus_tag="VH752_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEQ8289810.1"
                     /translation="MKTKKVIILVGLLLSSQLTLISCQSRGNGTYPIKTKQSRKGMTS
                     NKIKPIKKSKKTNKTHKGVAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSPFEYLIPKGASLAKPAVAQQAASQGTSKVADPHHHYEFNPADIVAEDALGY
                     TVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSSPVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAYVADQYDPAKKAEKPAETPHTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVETQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPDPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTPFPSNEKDPNKM
                     KEWLATVIKLDLGSRKDPLQRKGLSLLPNLETLGIGFTPIKDISPVLQFKKLKRLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPLASLHQLQELHIDNNQITDLSPVSHKESLTVVDLSRNADVD
                     LATLQAPNLETLMVNDTKVSHLDFLKNNPNLSSLSINRAQLQSLEGIEASSVIVRVEA
                     EGNQIKSLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPN
                     KTVTNIDISHNNIPLADLKLNEQHIPEAIAKNFPAVYEGSMVGNGTAEEKAAMATKAK
                     ESAQEASESHDYNHNHAYEDEEGHAHEHRDKDDHDHEHEDENEAKDEQNHAD"
     gene            5881..6822
                     /locus_tag="VH752_001469"
     CDS             5881..6822
                     /locus_tag="VH752_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEQ8289811.1"
                     /translation="MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQ
                     GIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPR
                     LYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYADMPPYTMMVASSIVKAISR
                     LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIA
                     QKAGSLFAEKPTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEK
                     RAIAIDLTDQGHLIVQFENGDFQTLRSGEISLSSWEN"
     gene            complement(6797..7000)
                     /locus_tag="VH752_001470"
     CDS             complement(6797..7000)
                     /locus_tag="VH752_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEQ8289812.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKTANSPKKKD"
BASE COUNT         2089 a         1497 c         1264 g         2202 t
ORIGIN      
        1 ataatattag ttagtgatat catttttata ttttttatga cagttaaaaa gccataaaat
       61 gcaacaaagt atgcacctaa gtatcattag cctaagcaca tgtaatataa aaaagagaag
      121 cgttagcttc tctttagtct tcgattaatt gaatatctgc ccctagtgct gttaattttg
      181 caatgatact agcatagcca cgtaagataa attcaatatt agtaatttct gtcttgcctt
      241 cagctattag accagcagtc actagagccg ctccggcacg taaatcagtg gctttgacct
      301 gagctcctgt caggcgagac ggcccttgat aaacaatctg accaccaata actgagatat
      361 ctgctcccat gcgcatcaac tctggcacat ggttaatgcg cttttcatat atagtgtcaa
      421 taatcgttcc acgaccatct gcttttaaca acaagggcgt taatggctgt tgcaaatcag
      481 tggcaaaacc agggtaagga gaggttttaa ttgtaatagc cttgagggat tcttgctttt
      541 caacaaagat agcatcctct tctaccgtca tacggacacc catttcttct aacttagcaa
      601 tgaaactctc taaatgctca taaagaacat tagtgatctt aacacctttt ccaatagctg
      661 ccgccaaagc gatataagtc cctgcttcaa tacgatcagg aataacttgg tgtctagtac
      721 catgcaattt ctgaacacct tgtatcgtaa taatatctgt acctgctcca cgaatatgcg
      781 cacccatgtt attcaaaaga gtagccacat caataatctc tggctcacgt gcagcgtttt
      841 caataaccgt tttaccttga gcttttgttg ccgcaaccat agtattaatt gtagcaccga
      901 cactaacggt gtccatatag atatgagcgc catgaatttt ttgaccattt gtcgataaat
      961 tcatattttc accctcataa gacacttcaa ctcccatagc ctcgaaggcc ttcaaatgca
     1021 agtcaattgg tcttggtcct aaatcgcaac caccaggtaa gccaacaact gcttgtccaa
     1081 aacgacctaa aagactaccg tagaaataat aggaagctcg aagactatta atctttccgt
     1141 aaggcatcgg gatgtcttgc acccctctag ggtcaatttc taaagtatca ccatgatagt
     1201 ttactgttgc tcccataagc tccatgattt caatcagact atctacatca ctaatagcag
     1261 gtacaccatc aagaatcaca atatcatcgg ctaagatgat tgctggaata agcgcaacaa
     1321 cactgttttt tgcccctgaa actgccactt ctccagataa ggcttttccg ccattaataa
     1381 taattttacg catacattca aaactttctt atactagaat tcaacctttt cattttatca
     1441 taaatcatga agcctgacaa ttattgacgt cttaattttt aatcatccct tttaaaggct
     1501 tatttaataa caaaaaagca gagtaagatt ttttcactta ctctactttt ttaataaagt
     1561 tgtcgtttat aatactgttt ttacagcttc tactaaagca tcaaccttat ttaaacgctc
     1621 ccatggcaaa tcaatatctg ttctccccat gtgaccatag gcagctgttt gtttgtaaat
     1681 cgggcgtttt aaatcaagca tttgaataat gcctgctggc cgtaaatcaa agacttgacg
     1741 aacagcagct tctaaaacag cttcaggaac cgttgaggtt ccaaatgtat ccacgcgaac
     1801 agacacaggc tgagccaccc caatcgcata agctagttgg acttcagctt tggtcgcaag
     1861 gcctgctgcc acaaggtttt tagcaatata gcgagcagcg taagaagccg aacggtccac
     1921 ttttgtcgca tcttttcctg aaaaagcacc gccaccatga cgtgaatagc ctccataggt
     1981 atccacaata atcttacgtc cggtcaatcc agagtctcct tgcggacctc caataacaaa
     2041 acgtcctgtt ggattaatga agaactttgt gtcatcatct aaataatctg ctggaattac
     2101 agccttgatc actttttcaa tcacatcctg acgaatttgg tcattagtag cttctggatc
     2161 atgctgagta gaaataacga ctgtatccac acgaacaggt ttatcgtgtt catcgtactc
     2221 aactgtcact tgagatttag catctggacg tagataacta atctcacctg acttacgtaa
     2281 ttccgccaag cgacgcacta attggtgaga taaggaaatt ggtaaaggca tcaattctgg
     2341 tgtttcattg atggcaaaac caaacatgag cccttggtca cccgctccaa tatggctaag
     2401 gtcatcagta tcaccttctc tgctttcgag agcttcatta accccctgag cgatatctcc
     2461 agattgttct acaagtgatg gatgtactcc cactgattct gctgaaaagc catattctgc
     2521 ttctgtataa ccaatttctg caatggtgtc gcggaccaca cggttaatat caatgtatgc
     2581 tgttgttgag atttctccaa aaacatgaac agaacctgtg taaacacaag tttctgcagc
     2641 cacatgggct tctggatctt ctgctaaaat ggcatctaaa atagcatctg aaatttggtc
     2701 tgcaatctta tctggatgcc cttcagatac ggactcagac gtgaaaagtt tacgttctga
     2761 cataaaaatg tcccccctaa tatgttgatt cttccttttt cagtcacatt gaccacaaag
     2821 caagaagttg cctacccctt tcttcctagc tgattgagca agtcaagtgg ccaaaaccaa
     2881 cttagctaat cagttacggt tttactagta aggcaactag ctagtccacc tagtggctag
     2941 cgtccattaa ccagacaaag aatgtctgac aatagcgttg agttacaaag aatccggtct
     3001 ccttgttgag aggatgtcac cacaaaataa gccttagacg aagtaattta gctaaagaga
     3061 gaacgactgt tccccaaatg aagtcaacaa cgtctcaaaa catttcgttc aacagaccgt
     3121 cttatcggga ctcaataaca atctattata acatgtttta gccatttagg tgccattctt
     3181 ttcactcatt atcctattta tttcaaaaaa acctcaaggt tattcttgag gttttcttga
     3241 tagtcaacaa tggtgtcatt aagacgattc agttggatcg ggttattctt agattagttt
     3301 agtcagcatg gttttgctca tctttagctt cattttcatc ctcatgttca tggtcgtgat
     3361 catctttgtc tctgtgctcg tgagcatgac cttcttcatc ttcataggca tgattatggt
     3421 tgtagtcatg tgattccgat gcttcttgag cactttcttt cgccttagta gccatagctg
     3481 ctttttcttc agctgttcca ttacctacca tagaaccttc gtaaaccgct gggaagtttt
     3541 tcgcaatggc ttctggaata tgttgctcgt tcaatttaag gtctgccaat gggatattgt
     3601 tatgactaat atcaatgtta gtaactgtct tattgggttt agataggttg acatttgtta
     3661 attggttttt agacacgctt aaaatgtcaa gtgctgtaaa attattaaca ccttctagag
     3721 aagtcaactg gttgcctgtc acatccaaga aagtaagtga cccttgcttg tctttaagca
     3781 caagcgattt aatttggtta ccttctgctt ctactctgac aatgacgcta cttgcttcaa
     3841 taccttcaag agattgcaat tgcgcacggt taatagatag gctagataga ttaggattat
     3901 ttttcaagaa atccaaatga gaaaccttgg tatcattgac cattaacgtt tctaagttgg
     3961 gtgcttgaag tgttgctaag tcaacatcag catttcttga taaatcaaca accgtcaatg
     4021 attctttatg agaaacaggg cttaaatctg taatctgatt attatcaatg tgcaattctt
     4081 gcaattgatg taacgatgcc agtgggctta aatcagaaat cttattatta ctcaatacga
     4141 ggaatttgag atttggtaat tgaccaagcg gcctaatatc ttcaatacca ttatcagcag
     4201 ccgctactag agttaagttt ttatatttgc tcaagaaact aatatctttg agattgtttt
     4261 gtgaaatatc aatgccttct aactgtggca tattatccaa aaatctataa tcagtcaccc
     4321 ctgtttttgt cattagtaac cgtttcaatt ttttaaattg caaaacaggt gagatatctt
     4381 tgattggtgt aaagccaatt cctaaagttt ctaagttggg taacagtgaa agtcctttac
     4441 gttgcaaagg atctttacgg ctgcccaagt caagtttgat aaccgttgct aaccattctt
     4501 tcatcttatt cggatctttt tcatttgatg ggaatggggt tggagcatcg tgagtgcgaa
     4561 cgatatccaa aatcacttct tcatcaaacc ctaaggcacg caaggtatcc attccaacct
     4621 tatgtttttc caattcacgg gcatgctcaa tagcatgtgg atctggaatg tcttttccga
     4681 tttcaatatc agataacatc aatacgtgtg cgtggtcatg gtgagggtat tccaaaccaa
     4741 gtttaccgtc ttgtgtttcc acacgtttaa tagttgatgg atcaatcccc aatttttctg
     4801 ccaaataagc taatttttct tggtattcct tttcacgttc agatagctct ggtgtgtgag
     4861 gggtttctgc tggcttttct gcttttttag cgggatcgta ttgatctgcc acatatgccc
     4921 agccaccctg acgaaggtcc gcaaaagaaa taggatgtaa gtgaccatcg tggtccacta
     4981 aaatactgtc ttttgttacc ccaacaatac cttgaccgtt aaattgaaaa ccatctgagg
     5041 ttgggaaatg caagcctgga ataccattag ctgtagctgt tgaaacaggg ctactggttt
     5101 gtggcaagcg agtagcaacc tgtttagctt gtgcctgtgt ctgacctgat aagcttgact
     5161 tcaaaatata atggaagtga tcatcgtggc gaaccgtgta gcctaaagca tcttcagcca
     5221 caatatccgc tgggttaaat tcataatggt gatgaggatc tgctacttta gaagtccctt
     5281 gactagctgc ttgctgagca acagctggct tagctaaact tgctcctttt ggaataaggt
     5341 attcaaatgg acttcccttt aaatcggcat aaaaaataaa atgcgaatga ccatcatggt
     5401 caacaacgat tccctgatct gtttttgata agatttttga gtctttggtt aaaataaacc
     5461 catcatctgt aggaaaatcg acacccgcca cacctttgtg agtcttgttt gtctttttgc
     5521 tttttttaat cggtttaatt ttgtttgacg tcattccctt acgtgattgt ttcgttttaa
     5581 tgggatatgt accattacct cgtgattgac aagatatcaa agtcaactga gatgataaca
     5641 atagaccaac taaaataata acttttttcg ttttcattca accctccttt ttatttaact
     5701 ggttaagtat atcacatcac ttgtttttcc tcaagctctt tttaggataa ctattagttg
     5761 ttgacgagtt ggagctctga agcttcaaca aacaatagtt tctgttgtta gtagcattac
     5821 acaaaaaaac taacttttct tttcctttga aaataagatc ttttataata tactatacat
     5881 atgaaaacat cagaaaaaat ttatcaatta ctgtcacaaa ccgatgactt cgtcagtggg
     5941 gaatatctag cagatcaact cagtatctct cgtacatccg tttggaaatc catcaaaagt
     6001 ctcgaaaatc aggggattca aattgattct ttaaaacaca agggctaccg catggtacaa
     6061 ggtgatattt tattacccaa aaccattagc caagggcttg ggatgccagt cacctatacc
     6121 cctcacagcc aatctaccca attagatgcc aaacaaggaa tcgaagctca taacagcgct
     6181 ccaaggcttt acctagctcc tagccaagaa gctgccaaag gacgcctcga tcggcaattt
     6241 ttttcagcta gcaccggtgg tatttacatg tccatgtacc taaaacccaa tgtcccttat
     6301 gctgatatgc ctccttatac gatgatggtt gcttccagta ttgtcaaagc tatctcgcga
     6361 ttaacaggca ttgatacaga aatcaaatgg gtaaacgaca tctatctagg taaccataaa
     6421 gttgcaggga ttctgacaga agccattact tctgttgaaa ctggtttaat caccgatgtc
     6481 attattgggg ttggtctcaa cttttttgtc accgacttcc cagaagcgat tgctcaaaag
     6541 gcaggttctc tctttgccga aaaaccgact atcacgcgca atgaccttat cattgacatc
     6601 tggaaattat tcttatctat tcctgtcaag gaccatgtca aagtttacaa agaaaaatca
     6661 cttgttctta ataaacaagt gacctttata gaaaatagtc aagaaaaacg tgctattgcg
     6721 attgatctca cagatcaagg acacctcatt gtacagtttg aaaatggtga cttccaaacc
     6781 ttgcgaagcg gtgaaatcag tctttcttct tgggagaatt agccgttttt aaaatggttt
     6841 tagtcaattg gttaatggta tgcactcttc ttaaatccct aaagagcaac aagccccaaa
     6901 ataaggcgaa gagataattt ccagagagca acaggtcatt aaaaaaatag gtttcaaaag
     6961 cacatacaaa ggccataaaa agaaattgtc gaatagtcat agctttatta taacagaaaa
     7021 aaatgattct atgaagaatc cacacagatt ga
//
LOCUS       DAUGHY010000058         7000 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.058, whole genome shotgun sequence.
ACCESSION   DAUGHY010000058 DAUGHY010000000
VERSION     DAUGHY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.058"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(17..289)
                     /locus_tag="VH752_001471"
     CDS             complement(17..289)
                     /locus_tag="VH752_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEQ8289813.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
     gene            complement(306..1673)
                     /gene="dnaB"
                     /locus_tag="VH752_001472"
     CDS             complement(306..1673)
                     /gene="dnaB"
                     /locus_tag="VH752_001472"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEQ8289814.1"
                     /translation="MRLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDD
                     FYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSA
                     NAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
                     FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGK
                     TAFVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWN
                     NVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKP
                     ENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDA
                     DIVAFLYRDDYYRKEGDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIA
                     QFEER"
     gene            complement(1703..2155)
                     /gene="rplI"
                     /locus_tag="VH752_001473"
     CDS             complement(1703..2155)
                     /gene="rplI"
                     /locus_tag="VH752_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEQ8289815.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLAITEA"
     gene            complement(2152..4128)
                     /locus_tag="VH752_001474"
     CDS             complement(2152..4128)
                     /locus_tag="VH752_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEQ8289816.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYQLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNSLIQQIITEKRREDISQTFEVSGNKYTSYIDVSSGIFYFFDSF
                     VGNRQLADASMLRPVVGIISVDNYDDITDDLSDADTSKINSFVANFIDEFMESKRIFY
                     RRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEAQDAQRPLTLSIGISFGEENHSQI
                     GQVALENLNIALVRGGDQIVIRENADHTNPIYFGGGSVSTVKRSRTRTRAMMTAISDR
                     IKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIENSFAVYNPDEMSPDIERAIERLQ
                     ADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTLSKEFYEQFQNVIVVDHHRRDDDF
                     PDNAILTFIESGASSAAELVTELIQFQNAKKRLNKIQASVLMAGIMLDTKNFSTRVTS
                     RTFDVASYLRSKGSDSVEIQNISATDFEEYKQINEIILQGERLGDSIIVAAGEKNHLY
                     SNVIASKAADTILSMAHVEASFVLVETASHKIAISARSRSKINVQRVMEKLGGGGHFN
                     LAACQLTDISLPQAKHLLLKTINMTMKETGEVES"
     gene            complement(4218..6116)
                     /gene="mnmG"
                     /locus_tag="VH752_001475"
     CDS             complement(4218..6116)
                     /gene="mnmG"
                     /locus_tag="VH752_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEQ8289817.1"
                     /translation="MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTE
                     IQPGDDKPNHFSFMSKDADYLKDQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGL
                     EKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRDIGLVDDERWKAFEIKKNQFDNELKRLNSIKLKPVKATNDRVQELGFKPLTD
                     AMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALDQVAKMK
                     RMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEG
                     NGKAHRKY"
     gene            complement(6240..6557)
                     /locus_tag="VH752_001476"
     CDS             complement(6240..6557)
                     /locus_tag="VH752_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEQ8289818.1"
                     /translation="MIVRNGKNFLTRDAADQYYTIGGTSLVGEKTHETVLRETLEEVG
                     IRAKVNQLAFMVENHFDIDDVFWHNIEFHYLVSPLEDPKLEMIENASDRFMNGFQSIN
                     WIK"
BASE COUNT         2130 a         1474 c         1087 g         2309 t
ORIGIN      
        1 ctctatgagg caaaaatcaa tctaaataac gaattaatgt tttttctgtc aaaatatctg
       61 aataggtgta tgattcgaca taagaattat caattgctcc tggagtatca gagctatcac
      121 tatactcaat gataaagagc gaactgtaaa cctcaataag cctaccaatt ttattttttt
      181 cccgtttccg tccattctca agggttaatt ctactaactg tccttcatgg gctcttatgt
      241 cctctttaat ttttttcatt tttgcaacat ctgtaaatgc atcgctcatt ttttgtctct
      301 ctttcttatc tttcttcaaa ctgggctata cttgagaatt tgttgtattc tttttgaaac
      361 atcagtttga ctgttccacg cgccccagct ctatttttct cgaggataac ttcaattgtg
      421 ttatcttcaa cagcttcttc agcatcatca ccttctttac ggtaataatc gtcccggtat
      481 aagaaggcta caatatcggc atcctgctca atagatcctg attcacgaat atctgataaa
      541 actggtcgtt tatcttgcct ttgctcaacg ccacgagaaa gctgacttag ggcaataact
      601 ggtactttca attctttagc taggatttta agctgtcttg aaatatctga aacctcttgc
      661 tgacgatttt cgggtttagt tcctgtaatc aactgtaagt agtcaattac gataagacct
      721 aaaccaccat ccacttcttg agacaatttc cgtgatcttg cgcggatttc agtaatttta
      781 atcccgggcg tatcgtcaat ataaatcggt gcttctgcca aagctccctg agcaattgtt
      841 acattattcc aatcctgatc tgtgagttgc cctgttctta aactgtgcga atcaaccatt
      901 ccttctgctg caagcatacg atctactaaa ctttcagcac ccatttccaa agaaaaaata
      961 gcaacagtct ttttttgctt agtccccaca ttttgcgcaa tattaagaac aaaggcagtc
     1021 ttccccactg ctggccgagc agctaaaata actaattgat ctgggtgtaa acctgttgta
     1081 atcttgtcaa ggtctctaaa accagttggt aaacctgtaa catttgaagt ctgcttagaa
     1141 cgtgcttcta aagcctcgta attaactttt agcacatctg aaattttgcg aaatccacta
     1201 cgattactat gttcattgag ttcaattaaa gctctctcaa ctccagcgat aacctcttct
     1261 ggttttaaaa tttcatcata agctaggttg acagattctg tcaacctagc aataatatca
     1321 cgcaacatag ctttctcagc tacaatttta gcataatatt ctgcattagc actagttggg
     1381 acactattaa ctagttcaac aatataggat aagccaccaa tactttgcag atcatcttga
     1441 tcatctagga ttgttcttat agtggttgca tcaatggcat cattacgatc gctgagggta
     1501 atcattgccc gaaagataat tttatgagcg tacttataaa aatcgtctgg actgataaat
     1561 tctctcactg caatcagctt atcaggtgag ataaagattg acccaagaac agattgctct
     1621 gctagtaaat cttggggttg aactcgtaat tcagctactt caggcaacct cataacctcc
     1681 aaatcttgtg atgtttttta tattatgctt ctgtgatagc caatttaatc tcagctgtta
     1741 cttccttatg taatttcaca ggaacttcaa ttagaccaat ggcgcggata ggatgatcta
     1801 aaacaatatg acgtttatca actttcacac caaattgctt ttgcaattct tctgaaatct
     1861 ttttagctgt gatggaacca aatgtacgtc catctgggcc aactttttct tgaaattgaa
     1921 cacgtgtttt gtcctcatcc aaaacagcct tgacagcttg agcttctgct aatatttcag
     1981 cttgagcttt ttcttcagct ttttgttttc cttttaattc accgatgctt tgactagttg
     2041 cttctttagc taaatttttc ttaataagga aattttgagc atagcctgtt gggacctctt
     2101 tgatttctcc ttttttacct tttcctttta catctgctaa aaaaataact ttcatgattc
     2161 tacttctcct gtttctttca ttgtcatatt aatagttttc aataataagt gcttcgcttg
     2221 gggaagactg atatccgtta actgacaggc agcaagatta aagtggcctc ctccacctaa
     2281 tttttccatc acacgttgaa cattgatttt actacgactt ctagctgaaa tagcaatctt
     2341 atgagaagct gtttcaacta atacaaagct agcctcaacg tgagccattg acagtatcgt
     2401 atctgcagct ttactagcaa taacattgct ataaagatga tttttttctc ctgcagctac
     2461 aatgatactg tcaccaagac gttctccttg taaaataatt tcatttattt gcttatattc
     2521 ttcaaaatct gttgcggata tattttgaat ttcaacacta tcacttcctt tgcttcttaa
     2581 gtaactagcg acatcaaagg tacgacttgt cactcgcgta gaaaaatttt tagtatcaag
     2641 cataatacct gccattaaaa cactagcttg gatcttattt aaacgttttt tagcattttg
     2701 aaattgaatt aactcagtga cgagctccgc agcactactt gctccacttt caataaaagt
     2761 taagatagca ttatcaggaa aatcatcatc tcttctgtga tgatcaacta caataacatt
     2821 ttgaaactgt tcataaaatt ctttagaaag tgttaaagat atcttagaat ggtcaaccat
     2881 taccaaaagt gatcttggag tcactaaacc cattgcttga gatacactga ttagacgagt
     2941 ctttccgtca gcctgcaatc gctcaatagc cctttcaata tctggactca tctcatctgg
     3001 attgtaaacg gcaaaactat tttcaataat gttacctgcg aaaaattgca taccaacagc
     3061 tgaacctagt gcatccatat caagtttcct atgtccaaca ataaaaacat tgtccaccat
     3121 cttgattcga tctgaaatag ctgtcatcat agcacgggta cgtgttcttg aacgtttaac
     3181 tgtagaaaca gaccctcccc cgaaataaat tggattcgta tgatccgcat tttcacgaat
     3241 gacaatctga tcaccaccac gaacaagcgc aatattaaga ttctcaagag ctacctgtcc
     3301 aatctggcta tgattttctt caccaaaaga aattcctatg ctgagtgtta aagggcgctg
     3361 agcatcttga gcttctttac gaaactcttc taagacagaa aatttattat ccatcaaatc
     3421 atttaatgtt ttaaagtctg taaagaaata gtagcgatcc atattaacac gacggtaaaa
     3481 aatccgtttt gactccataa actcatcaat gaagttagct acaaatgaat taatttttga
     3541 ggtatctgca tctgataaat catcagtgat atcatcataa ttatcaaccg aaataatgcc
     3601 aacaaccggt cttaacatac tagcatcagc taattgcctg tttcccacaa agctatcaaa
     3661 aaaataaaaa attcctgatg atacatcaat ataagaagtg tatttattac cagaaacttc
     3721 aaatgtttga gaaatatctt cacgacgttt ttccgtaata atttgctgaa tcaaactgtt
     3781 ttgtataaaa ccctcttcag ttgtaaaaat taattctgca taaggattat accattctac
     3841 agcgttggtc tcttgatcaa attgaactac tccaacaggc atattatcaa gtagcgtttt
     3901 taggttatct tctgtttgtt catttaagag ctcaatatga gctaagtctg ataattgata
     3961 agcttctttt tgataccata aaagagcaac aacaaatagc aaaacgagaa aaatagctaa
     4021 taatattaat atttttgatt gcataatact gacacaaagt gccagtagtc caaataaaat
     4081 caatcccatc ataatcaaat gaatggtttc aaaacgaaat tttttcatct tataacctct
     4141 taagcacaca ttataccata aaaaagccct gaaatacaga gcttccactt gctttgagat
     4201 tacaatttca tttccattta atattttcga tgagcttttc cgtttccctc taaataaatc
     4261 atcaagatac tgatgtccgc aggattgact cctgaaatac gactagcttg tccaattgtt
     4321 tcaggattaa ttttcttaaa tttctgacgc gcctcagttg caatagaatc aatagcatcc
     4381 caatcaatat ttgcaggaat acgtttttct tccatgcgtt tcatcttagc aacctgatct
     4441 aaagctttgc gaatatagcc ttcgtacttg atttctgttt ctaaaagttc aatgatttta
     4501 gcatctaaat cttcagccgc aggaccaaca aaagagactg cagtcgcata atcaatttct
     4561 gggcgacgca taaattcttt agctgtcata gcatccgtca atggtttaaa accaagttcc
     4621 tgcacacgat cgttggttgc ttttactggt tttaatttga tactgttcag acgttttaat
     4681 tcgttatcaa attggttttt cttaatttcg aaggccttcc aacgctcatc atcaaccaac
     4741 ccaatgtcgc gaccaatctc tgtcaaacgc atatcagcgt tgtcatgacg taaaatcaaa
     4801 cgatactcag ctcgcgatgt caacaatcga taaggttcca aggttccttt ggtaaccaaa
     4861 tcgtcaatca taacgccaat ataggcatcg ctccgtttca aaataagttc tggcttacct
     4921 tgaactttca aagcagcatt gattccagca ataagtcctt ggccagcagc ctcttcatac
     4981 ccagaagttc cattagtttg cccagccgta aagagtcctg aaattagctt ggtttcaagt
     5041 gtggcacgta gctggtgagg taaaacaata tcatattcaa tagcataacc agtgcgcatc
     5101 atttcagcct tttcaagtcc tttgatcgaa tgaatcaaat ctttttggac atcttctgga
     5161 agactcgttg acaacccttg aacatacact tcttctgtat cacgcccctc tggctctaag
     5221 aaaagttgat gacgttcttt atcagcaaag cgaacaatct tatcttcaat agatgggcag
     5281 taacgagggc ctacaccttt aacaatgcca gaaaacatcg gtgctctgta aaggttctga
     5341 ttaatgatat catgactagt ttgattagta taagtcaacc agcagggaat ttgatctttg
     5401 aggtaatcag catcttttga cataaatgaa aaatgattag gtttatcatc acctggctga
     5461 atttccgttt gatcgtaatt aatagaagat gctttgacac gaggaggcgt tcctgttttg
     5521 aatctgccaa tttctaagcc gagttttttt aaattatctg ctaaggttac tgatgccaaa
     5581 ctattattag gcccagaaga atatttgagt tctcctaaaa taatttcgcc acgcagagct
     5641 gttcctgttg tcactacaac tgccttagct gcaaactttt gtcctgtggc tgttaaaaca
     5701 cctacaactc gtccatcttc aactagaata tcatcaatca tcgtttggcg aagagtcaaa
     5761 ttagcctgtt tttcaaccgt atgtttcatc tcacgcgcat acagggattt atctgcttga
     5821 gctcggagag cgcgtacagc tggtccctta cctgtattaa gcattttcat ctggatatag
     5881 gttttatcaa tatttttacc catttcgccg ccaagagcat cgatttctcg tactactatc
     5941 cctttggcag atcctccaat agaaggatta cacggcataa aagccaacat atcaaggttg
     6001 attgttgcca ataaagtttt acaacccata cgactagtgg ccaaactggc ttcaacacca
     6061 gcatgacctg cacctatgac aatgacgtca tagctttctg taaattcatg tgtcattttt
     6121 ttaaaatcct ttatttcagt ctattcattt aaaatcatac gctttagttt agccttcaat
     6181 gcggacaata tgtcctggcc atttagccaa ctcggtttgt aaaaactcag gaacaagatt
     6241 catttgatcc agttgattga ttggaatcca ttcataaatc tgtcgcttgc attctctatc
     6301 atttctagtt tagggtcttc taaggggctt accaaataat gaaattcaat attatgccag
     6361 aaaacatcat cgatatcaaa atgattctca accataaaag ccagttgatt aaccttagct
     6421 cgaataccaa cttcctctaa ggtttcacgt aaaacagttt catgcgtttt ttcacctacc
     6481 aaacttgtac caccaatggt gtaatattgg tcagctgcat ctctagttaa aaaattctta
     6541 ccatttctaa caatcaagcg ctagctcgta aactaaagtt atctgacctc tagtgatttt
     6601 aaattccagt cctattagcc atctcatttc tagagctaaa ccataaaagg gccaaaaccc
     6661 tagaagattg caggacaaaa aaacctacaa ctttcatgag ctttgaccat cattccgatt
     6721 gttactatct attttaaaaa aatgattagt gattgtcaat attgatttca tcacagacct
     6781 ttacagtaat gtcaatgttt ttacagctct gtcaatctat tgtaatgttt ttaaaatgat
     6841 tacaaaagta tcactatgtc taatctagcg aggcgagaaa taaaaatgtc atcagtatta
     6901 tcatacgatt ttttgatatc ttctaaagta gtaaaataca gataccgttg tttctaagtc
     6961 tacaagtaag aacgccgata acaaaaccat aaaagtataa
//
LOCUS       DAUGHY010000059         6870 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.059, whole genome shotgun sequence.
ACCESSION   DAUGHY010000059 DAUGHY010000000
VERSION     DAUGHY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.059"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            97..1515
                     /locus_tag="VH752_001477"
     CDS             97..1515
                     /locus_tag="VH752_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ8289819.1"
                     /translation="MDKKIQRFSCTTILVGKKASYDGSTMVARTEDSQNGDFTPKKMI
                     VVKPEDQPRHYRSVQSSFEMDLPDNPMTYTSVPDALGKDGIWAEAGVNEANVAMSATE
                     TITTNSRVLGADPLVASGIGEEDMVTLVLPYIRSAREGVLRLGAILEDYGTYESNGVA
                     FSDEHDIWWLETIGGHHWIARRVPDDAYVTNPNQFGIDYFEFNNPEDYLCSADLKDFI
                     DTYHLDLTYNHEHFNPRYAFGSQRDKDRHYNTPRAWIMQKFLNPEIVQDPRSFALAWC
                     QKPYRKITVEDVKYVLSSHYQDTVYDPYGSEGTPVSKKVFRPIGINRTSQTAILHIRP
                     NKPQEIAAIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVSTDNFYWTNRLIAAL
                     ADPHYNHHETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQ
                     TLLNKILFDASNLMTNRFSLSD"
     gene            1667..3214
                     /locus_tag="VH752_001478"
     CDS             1667..3214
                     /locus_tag="VH752_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEQ8289820.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPVYEF
                     TKGVIGNDGDVSMLMKAGTEPHDFEPSTKDIKKIQDADAFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGVGHDHHHEEADKKHEHNKHSEEGHNHAFDPHVWLSPYRS
                     ITVVENIRDSLSKAYPEKAENFKANAATYIEKLKELDKDYTAALSDAKQKSFVTQHAA
                     FGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTL
                     AKEAGVKAAVLSPLEGLTEKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTK
                     TVYNGYFKDKEVKDRQLSDWSGSWQSVYPYLQDGTLDQVWDYKAKKSKGKMTAAEYKD
                     YYTTGYKTDVEQIKINGKKKTMTFVRNGEKKTFTYTYAGKEILTYPKGNRGVRFMFEA
                     KEPNAGEFKYVQFSDHAIAPEKAEHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREI
                     AQEINAH"
     gene            3362..4084
                     /locus_tag="VH752_001479"
     CDS             3362..4084
                     /locus_tag="VH752_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8289821.1"
                     /translation="MPKEQPLYLQIVDDLEVKIKKSMTENDKLLSERELSDLYGVSRI
                     TIRLALKELELRDLIYKKQGKGTYVSGIKEPVTDLSSTYSFTEQMKKVGKTPKTEILS
                     FEQYQVTPYLSGLLEFDLDTEVFELERLRIADDMPLMFERSYIPAQPFQGLSIADLKR
                     KALYDIFAKDYQETIRLAEEEFYASIALDYEADLLGIKKGDPVLHIIRKTYNDKNILI
                     EFTFSIARADQFRYRVQHHPNG"
     gene            4104..5303
                     /locus_tag="VH752_001480"
     CDS             4104..5303
                     /locus_tag="VH752_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEQ8289822.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFEETRDSLDS
                     FLKRVCKSANGNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQCYLFESVASTDLVAAP
                     DYYLAEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEAYVTDMILAGQAVI
                     DQERRLQELADLGFERLVYLGSAGLAKLTQEAQLKMLELTAGQVATLYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKILALLQGGDINFSEDQFRL
                     DTRHLLPEAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGTVNRVVKGVTIHPYPTS
                     SRQCNKA"
     gene            complement(5400..5660)
                     /locus_tag="VH752_001481"
     CDS             complement(5400..5660)
                     /locus_tag="VH752_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEQ8289823.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
     gene            complement(5775..6716)
                     /locus_tag="VH752_001482"
     CDS             complement(5775..6716)
                     /locus_tag="VH752_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEQ8289824.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLTWISQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGQCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
BASE COUNT         2155 a         1289 c         1438 g         1988 t
ORIGIN      
        1 aataataaga taaggaggtg ctgctggaat tatgaaaatt tttgtatcca gttcaatgga
       61 tatttgatat aatcgtctta aaaaggaggt cgtgaaatgg ataaaaaaat acagcgtttt
      121 tcatgtacaa ctatactcgt cggtaaaaag gcatcttatg atggctcaac catggttgct
      181 cgaacagaag attctcaaaa tggtgatttc acgcctaaaa aaatgattgt ggtgaaacca
      241 gaagatcaac cgagacatta ccgttcggtg caatcttcct ttgaaatgga cttaccggac
      301 aatcccatga cttacacgtc tgttcccgat gcgcttggta aagatggtat ctgggcagaa
      361 gcaggcgtga acgaggctaa tgtggccatg tcagcaactg aaaccatcac taccaattct
      421 cgtgttttag gggcagatcc tctagtagct tctggtattg gtgaggagga catggtgacc
      481 ttagtgctcc cttatatccg ttctgcgcgt gaaggggttt tgcgccttgg tgccatttta
      541 gaagactatg gcacttacga gtcaaatggt gtggcttttt cagatgaaca tgacatttgg
      601 tggctggaaa cgattggtgg tcatcactgg attgcaagac gggtgccaga tgatgcgtat
      661 gtcaccaatc ccaatcaatt tggcattgat tattttgaat ttaacaatcc tgaagattac
      721 ctttgctcag ctgatttaaa ggactttatt gacacgtatc acttagattt aacctataac
      781 catgagcatt ttaatccccg ttatgctttt ggtagccaac gtgacaaaga ccgtcactat
      841 aatacgccac gtgcttggat catgcaaaaa ttcttaaacc cagaaattgt gcaagacccg
      901 cgcagctttg cccttgcttg gtgtcaaaaa ccttaccgta aaataacagt agaagatgtc
      961 aaatatgtat tgagtagtca ctatcaggac acggtttatg acccttacgg ttcagaagga
     1021 actcctgtta gtaagaaagt ctttcgccct attggcatta accgtaccag ccaaaccgct
     1081 atcttacaca ttcgtcctaa taaacctcaa gaaattgcgg ctatccagtg gatggcttat
     1141 gggtcaatgc catttaatac catggtgcct tttttcacac aggtgaagac tattccagat
     1201 tattttgcca acacttacga aaatgtgtct accgacaatt tctattggac caaccgtctg
     1261 attgcggctt tggcagatcc tcactacaat catcatgaaa ctgatttgga caattacttg
     1321 gaagaaacca tggcaaaagg tcatgccatg ttacatgctg tggaagcaca gttattggct
     1381 ggtgaaacag tcgatttgga agaggaaaat caaaaaatga gtgactatgt gcaaggagaa
     1441 acgcagacgc tgttgaacaa gattctcttt gacgctagca atctaatgac caatcgtttc
     1501 agtttgagcg attaaaagtg aagctaaggc tgagcaactg cttagccttt tttacgataa
     1561 ctttttagga aaagtaatcg caaaaagaca aaaacttgga aaaaaaatca aagtatgcta
     1621 tacttaactg gtcaattttt aaccagttaa taacaaagga ggaatcatga aaaagaaaat
     1681 tcttttaatg atgagtttaa tcagtgtctt ttttgcttgg caacttactc aggcaaaaca
     1741 agtcttagca gagggtaaag tgaaggtggt gacaactttc tatcctgttt atgaatttac
     1801 aaaaggggtt attggtaatg atggcgatgt ttccatgctt atgaaagcag gaacggaacc
     1861 tcatgatttt gagccttcta caaaagacat taaaaaaatc caagatgcag atgcatttgt
     1921 ttatatggat gacaatatgg aaacttgggt ttctgatgtg aaaaaatcat tgacatctaa
     1981 aaaagtgacc atcgtcaagg gaactggtaa catgctcttg gtagcaggag ttggacatga
     2041 ccatcaccat gaggaagctg acaaaaagca tgagcataat aaacatagcg aagaaggaca
     2101 caaccatgct tttgacccac acgtgtggtt gtcaccatac cgtagcatta cggtcgttga
     2161 aaatattcgc gacagtcttt caaaagctta cccagaaaaa gcagagaact tcaaagccaa
     2221 tgccgctact tatattgaaa aattaaaaga gcttgacaaa gactatacgg cagcactttc
     2281 agatgctaag caaaagagct ttgtgacaca acacgcagct tttggttata tggcacttga
     2341 ctatggcttg aaccaaattt ctattaatgg tgtcacacca gatgcagaac catcagcaaa
     2401 acgtattgct actttgtcaa aatacgttaa aaaatatggt atcaaataca tttattttga
     2461 ggaaaatgcg tcaagtaaag tcgcaaaaac cctagctaaa gaagcaggag ttaaagcggc
     2521 tgtgcttagt ccgcttgaag gtttgactga aaaagagatg aaagctggcc aagattactt
     2581 tacggtcatg cgtaaaaacc ttgaaacctt acgcttaacc actgatgtgg ctggtaaaga
     2641 aattcttcca gaaaaagaca cgactaagac agtttacaat ggttatttca aagacaaaga
     2701 agtcaaagat cgtcaattat ctgactggtc aggtagctgg caatctgttt acccctatct
     2761 acaagatggt actttagacc aagtttggga ctataaggct aaaaaatcta aaggtaaaat
     2821 gacagcagcc gagtacaaag attactacac tactggttat aaaactgacg tggaacaaat
     2881 caaaatcaat ggtaagaaaa agaccatgac ctttgttcgt aatggtgaaa agaaaacctt
     2941 cacttacaca tacgccggca aagaaatctt gacctatcca aaaggaaatc gcggagttcg
     3001 tttcatgttt gaagctaaag aaccaaatgc tggcgaattc aaatacgttc aattcagtga
     3061 ccatgccatt gctcctgaaa aagcagagca tttccacctg tactggggtg gtgacagcca
     3121 agaaaaatta cataaagagt tagaacattg gccaacttac tacggttcag acttatctgg
     3181 tcgtgaaatt gcccaagaaa tcaatgctca ttaatcttaa ctcactaagc aaatcttctt
     3241 ttaaggaggt ttgctttttc tttgtggagt aaaaaaattt aataattctc aacattaatt
     3301 tgacggacac ttatgattat aatatacttt aagtggttat aacaagttag aggtgaataa
     3361 gatgccaaaa gaacaaccac tctatctaca gattgttgat gacttggaag tgaaaatcaa
     3421 aaagagcatg acagaaaacg ataagttgtt gtcagaacga gaattaagtg acctttacgg
     3481 tgttagtcgt atcacgatta gactggctct taaggaattg gaattacgag acttaattta
     3541 taaaaagcag ggcaagggga cttatgtttc aggaatcaaa gaacctgtca ctgatttatc
     3601 aagtacttac agctttacag agcaaatgaa aaaagtgggg aaaactccta aaacagagat
     3661 tctttctttt gaacagtatc aggtaacacc ttacttgtct ggtctcttag agtttgatct
     3721 cgatacagaa gtttttgaat tagagcgcct aaggattgca gatgatatgc ccttgatgtt
     3781 tgaacgatcc tatattcctg cgcagccttt tcaggggtta agcattgcgg acttgaagcg
     3841 taaagctttg tacgatatct ttgcaaagga ctatcaagag acaatccgtc ttgcagaaga
     3901 agagttttat gccagcattg ccttggacta tgaagcagac cttttgggaa tcaaaaaagg
     3961 agatcctgtc ttacatatta ttcgtaaaac ttataacgat aaaaatattt tgatcgaatt
     4021 tactttcagt attgcaagag cagaccagtt taggtatagg gtacaacatc accccaatgg
     4081 ttaaagaaat gggaggttaa tacatgttta caaaaacgca ggaagctttg gaggctcttg
     4141 gtgcagctat cactaccaag gaaattaaac aagaaccccg tttatggcaa gaaaccatga
     4201 cgttttttga agaaacaaga gattctctgg attctttttt aaagagagtt tgtaaaagcg
     4261 ctaatggaaa taacgttcat gtcatcttta caggtgcggg tacatctgag tacatcggta
     4321 ataccatttg cccttacttg aagaaggtgg ggaatcggca gtgctatctc tttgagagtg
     4381 ttgcttcaac cgatttggtt gcggcgccag attattatct tgctgaggaa gagacggtac
     4441 tattagtctc ctttgctagg agtggtaata gtccagaaag tgtagcggct gttaacctgg
     4501 tcaatcaact agtgccaaat agttatcatt taaccattac gtgtgccaaa gatggagaac
     4561 tggcgaaaaa agcacagcaa gatgagcgtt cttaccttta tttgatgcct gaagacgcta
     4621 atgatgctgg ttttgccatg actggtagtt ttacctgtat gatgctagcg gctcttttga
     4681 tttttgatga tgctaacagt atagctcaga aagaagctta tgtgactgat atgattcttg
     4741 ctgggcaagc tgttattgac caagaaagac ggctacaaga gctagctgat ttaggatttg
     4801 aacgtttagt ttatctaggt tctgctggtt tagccaagtt aacccaagaa gctcaattaa
     4861 aaatgttaga attgacagct ggacaagttg cgaccctcta cgagtcctct atgggcttta
     4921 ggcatggtcc gaaatctttt attaatgacc gtaccttagt cattggattt gtcaataatg
     4981 atgcttacgt cagacagtac gatttggaca tgttagagga aatccaagca gatggcattg
     5041 cccttaaaat cttggctcta ttgcaaggag gggatatcaa cttttcagag gaccaatttc
     5101 gactagacac ccgtcactta ttgccagaag cttacttagc ttttcccatg attttagtcg
     5161 cgcagacctt ggccctctta acggccgtta cattaggaaa ttcccctgac accccatctg
     5221 cgacaggaac ggttaatcgt gtggtcaaag gagtaacgat tcacccttac ccgactagta
     5281 gtcgtcaatg caacaaagct taaagctttt gtgtagaagc cgtaaaacga aatgattttg
     5341 tgctagtagg ccaaaagaaa aaccctcctg atacaggaag gttttttgta ttgtcttgct
     5401 tattttgctg cgtttgcgtc tttgagaccg tattttttgt tgaagcggtc cacacgtcca
     5461 tctgcttgag tgaacttttg acgtcctgtg tagaatgggt gtgaatctga tgaaatttcc
     5521 acacggataa gtgggtaagt ttcaccttca aactcaacag tttctttaga agcttttgtt
     5581 gagccgctaa ggaactggta acctgtagtt gtatcaagga aaacaactgg acgataatct
     5641 ggatgaatgt cttttctcat cttaaaaaat tcctttctgc catgacactt gatgcatcat
     5701 agttattaac tattctagtg taacaaaact tgaatgactt gacaaggctt ttctttcctt
     5761 attttttaaa aatcctaaat ctcttggcaa acagcgatga gttctcggaa aatagcttga
     5821 ttttcttcta aattagctga gttggctccg ctagcaaggg gatgcccccc tccaccgtga
     5881 cgcttagcaa tgccattaat aatcttttct ttactgcgca tacgcacacg gtagttgccg
     5941 tcagttaact caacaaaaat agcccaagct tgaacgttat caattttacc aggagcacag
     6001 acaatggcag agctttctgc tagggtcacg tcaaaatgtt ttaaggtttc ttggctgaca
     6061 aggacataag cagccccact ctcatcaatt gttaaatgct caaagacgta gctttgcagc
     6121 tttgctattt tcaaaggaaa cgaatccatt tgccttgaaa tcgcagcaaa gtcgaactcg
     6181 aaatgtctga gttggctagc aatggaaagg gttttactag tggttgaggc ataaagaaat
     6241 cgccctgtat caccaacgat accagtgtat aagagcttag cagccttgtc agagagagta
     6301 agattctgac taaaggcaaa gtctgcaatg atttcacttg cgctagaagc gcttgtgtcc
     6361 acataataga agtcaccata cacatcatcg ttgggatggt gatcaatctt aattaagcac
     6421 tgccccagtg tgtagcgctc atcatcaatc cttggtcgat tcgctgtatc tgtaatgatg
     6481 accaaagcct ctttatagtc tttatcagtc acctgatcca tttggctaat ccaagttaaa
     6541 ctaggctcat caaaaccagt catcaaaacc tttttatctg ggaaattttg tgcaataatt
     6601 tctttcaagc cggcctgact accaagagca tcagggtcag gattttgatg gcgatggata
     6661 ataatagttt ggtgagcttt tattttatct aaaattgttt caaaagttgt tatcatgctt
     6721 taatctttct ttttcaaaaa gaggaaaata accgtttaac atagtcttca aaaaacggtt
     6781 tgtgtcataa ggaggtgtgc gttatgttaa gtggctaatg ctagaatttt ttaagattgt
     6841 caaagctcat aaattgacat tttatctgaa
//
LOCUS       DAUGHY010000060         6795 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.060, whole genome shotgun sequence.
ACCESSION   DAUGHY010000060 DAUGHY010000000
VERSION     DAUGHY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.060"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            77..808
                     /gene="aphA"
                     /locus_tag="VH752_001483"
     CDS             77..808
                     /gene="aphA"
                     /locus_tag="VH752_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEQ8289825.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
     gene            986..2518
                     /locus_tag="VH752_001484"
     CDS             986..2518
                     /locus_tag="VH752_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEQ8289826.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVIVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGSWLHLPAYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVVSGNTRLLSGATIFLVVNEADTGFVREV
                     LM"
     gene            2681..3295
                     /locus_tag="VH752_001485"
     CDS             2681..3295
                     /locus_tag="VH752_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEQ8289827.1"
                     /translation="MLEIVSEELRHYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGLGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKDLAMIPT
                     IEFINLNTCLKDEKGGLSDENTLDGLHLNFPAYAKLAEIIQAYI"
     gene            3454..4602
                     /locus_tag="VH752_001486"
     CDS             3454..4602
                     /locus_tag="VH752_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ8289828.1"
                     /translation="MIIKGVTMKFEKKQVFYLVLTFILCYGILANWRNGTAIVTTIYK
                     TSLPFFYGAAGAYIVNIVMSAYEKVYVYIFKDWSHVLKVKRGICLLLAYLTFFILITW
                     IISIVIPDLITSISTLTKFDTITIQEVVNNLEHNKLLARTIQYIGGDGKLTETIANYS
                     QQLLKQFLTVLTNILTSVTVIASAIINLFISFVFSLYVLASKEDLCRQGNTLVDTYTG
                     KYAKRIHYLLELLHQRFHGFFVSQTLEAMILGSLTASGMFILRLPFAGTIGVLVAFTA
                     LIPVIGASIGAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNFIYPKVVGGSIGLPAM
                     WVLMAITIGASLKGIVGMIIAVPLAATLYQVIKDNIQKRQAIQKKQVS"
     gene            4651..5331
                     /gene="radC"
                     /locus_tag="VH752_001487"
     CDS             4651..5331
                     /gene="radC"
                     /locus_tag="VH752_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEQ8289829.1"
                     /translation="MYSIKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHV
                     LELSSYLLSHLDSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTD
                     SVLTSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIIEEKTIFIGTVRRSLAEPREILY
                     YACKNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYS
                     FREKSTLF"
     gene            complement(5333..6028)
                     /locus_tag="VH752_001488"
     CDS             complement(5333..6028)
                     /locus_tag="VH752_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEQ8289830.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEEKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTVAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(6038..6682)
                     /locus_tag="VH752_001489"
     CDS             complement(6038..6682)
                     /locus_tag="VH752_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEQ8289831.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQEVTDQLIEVGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
BASE COUNT         2141 a         1058 c         1304 g         2292 t
ORIGIN      
        1 gtactattat aagaatttga aaccgcttta cttaaatcgt gtatttttgt acaataatga
       61 ttaaggaggt ttttagatga aaaaagaatt taccagtatc ctgtttacag ttagtttttg
      121 tggaatcata gcattgcctg tcgaagctag tggccctaaa gttccttata cacaagaagg
      181 tataacggcc atttcaaatc aggcaacagt aaaattgatt tctattgctg atatcgcgag
      241 cagtttagaa ggacaaaaac ctataacggt tagttttgat atcgatgata ctttgttatt
      301 tactagccaa tattttcagt acggcaaaga atatataact ccaggctcat ttgatttttt
      361 gcataagcaa aaattttggg acttggtcgc taaacgggga gatcaggatt ctattccaaa
      421 agagtatgca aagcaactaa tcgcaatgca tcaaaaacga ggagataaaa ttgtctttat
      481 tactggccga actcgaggat cgatgtataa aaaaggtgag attgataaaa cagctaagtc
      541 actggctaaa gattttaagt tagacaagcc aatagctatc aattatacag gagataaagc
      601 tgtgaagccg tatcagtatg ataaaacata ttatataaag aaaaacggct cacaaattca
      661 ttacggagat agtgatgaag atataaatgc tgccaaagag gcaggtgcaa gacctattcg
      721 cattttaaga gctcctaatt caaccaatct tcctttgcca aaagctggag gatacggaga
      781 agaggtttta gaaaattcgg cttattagtc aaatatttgt gtaagaaagg aagtaacggt
      841 aatttctttc atcaccccac tgagtaacca atagcccagt gggttttttg ggttacagaa
      901 atcagttcta aaaaagaatc actggtaact taaggttgtc tttgatacaa tggtaacagt
      961 gtatttaaaa gaaagagagg acaacatgga aaaccataaa aatgaattca ccttttccaa
     1021 caaatcaatt attgcctatg tttggcgtgg tgttatagtt ggtattattg ctggtgttat
     1081 tgttagttta ttccggttgt taattgaagt gacagctgac tgggtgattg aatggtaccg
     1141 atacgctcat atcaattcgc tgttgctact tcctatacta agtgtcagtt tattagctgt
     1201 cctttttgta ggttttttag taaagtctga ttctgatata aaaggatcag ggatccccca
     1261 tgtcgaagga gaactaaagg gactaatgtc cccagattgg tggagtgtcc tttggaaaaa
     1321 atttttagga ggaatcatgg ctatttctat gggatttatg ctcggtcgtg agggtccttc
     1381 gattcagtta ggtgctatgt cagccaaagg attagctaag tttttaaaat cgagtcgttt
     1441 ggaaaagcga gtgctaattg ccagcggagc agcagcaggg ctatcagcag cttttaatgc
     1501 tccaatagca ggcctcttgt ttgttgttga agaaatttac catcattttt cgcgtttaat
     1561 ttggattaca gcgctagtgg ctagcttagt ggctaatttt atttctctga atatttttgg
     1621 attgaaacca gtcttagcaa tgtcagaggc gatgcctttt ttaggactca atcaatattg
     1681 gttgttgctt ttacttggat tatttctagg ctgcttaggc tatttatatg agatagttat
     1741 ccttaatttt aataaacttt atgttatttt agggagttgg ttacatctgc cagcttattt
     1801 ttacggtatt attatggttt ttctaatttt accgattggc tattatttac cacagttact
     1861 tggaggagga catggtttaa tactttcttt atccaatcaa caattgcctt tgatgactat
     1921 cttcttttat tttattattc gatttattgt tagtatgttt agttatggaa gtggcttacc
     1981 tggaggtatt tttttaccta ttttaacttt aggtgctttg gcaggcctat tatttgggca
     2041 aatagcttct caattaggtt tattgaacca atcttttctt tctctttttt tgatattagg
     2101 gatggcaggg tattttgcgg caatttcaaa agcaccactg acaggcatga ttttggtaac
     2161 tgaaatggta ggtgatttga aacccttaat ggccatagct gtagttactt ttgtctccta
     2221 tttagtaatg gatttgttaa atggacagcc aatttatgaa gctatgctag ataagatggc
     2281 aatgaagcat ccaactaatc ttgttgaacc aacattaatt gaattgactg ttggtgataa
     2341 gattgcaggg aaatatgtta aagagttaaa gcttccagaa aatgtcttaa ttacaaccca
     2401 aattcaccat caaaaatctc aagttgtgtc aggtaacacg cgccttttat caggagcaac
     2461 tatctttttg gtcgttaatg aagctgatac aggttttgtc cgtgaggtat tgatgtaggt
     2521 attaatttat cacaattcgg aaagttcgag cttgtctttt agactaagtc ttcgaacgtt
     2581 ttactgctag agtttcatct gaaccaaagt gaaacgcttt tgatgaagtc aagtaggctt
     2641 tttgttatac taacaataaa gataagctaa ggaggctgag atgttagaaa ttgtttcaga
     2701 agagctacgt cactatcaag aacaaaaatt gattgagtac cgtaacaaaa atcagttagc
     2761 cccaaaagga ggcattgtat ttgcaggtga ttctctcatt gaattttttc ctttaaaaaa
     2821 agcttttggt tcatgtttgc ctattattaa tcgaggtatt gctgggatag atagtcagtg
     2881 gttattgcgc catttttctg tccaaattac tgatttagag cctaaacata tttttttatt
     2941 aattggttgt aatgatatcg ggcttggata tgataaatgt catattgtca agactattgt
     3001 agagcttatt agccagattc gtagtcattg tgtctacagt cagatttatc tcttatcttt
     3061 attaccagtt tccaacaatc cccgatatca gaagactgtc aaaataagaa caaatgctat
     3121 gattgatgct attaataaag atttagcaat gattccaact atagaattta ttaatcttaa
     3181 tacttgtctt aaagacgaaa aaggtggttt atcagatgag aatactctag atggtttaca
     3241 tcttaatttt ccagcttatg ctaaactagc tgaaattatt caagcttata tctagcaaat
     3301 aaatgtacta atagcgatga ggcaagaagt cagatagcag atggttcatc aatcaattaa
     3361 gaaaaaagtt atcaagatac cattaaaaat agttgctcaa agttctttca aatcaaaaga
     3421 ttatgatatc gctatgttga gttttagtgt ataatgataa ttaaaggagt taccatgaaa
     3481 tttgaaaaaa aacaagtctt ctatttagtc cttacgttta tcttatgcta tgggatccta
     3541 gcaaactggc gaaatggaac ggctatcgtc acgactattt acaaaacaag ccttcctttt
     3601 ttttatggag cagcaggtgc ctatattgtt aatattgtaa tgagcgctta cgaaaaggta
     3661 tatgtttata tctttaaaga ctggtcacat gtcttaaaag taaaacgggg tatttgttta
     3721 ctgttagcgt atttgacatt ttttatttta attacttgga ttatttccat tgtcattcca
     3781 gacttaatta caagtattag tactctaaca aaatttgata ctataacgat acaagaagtt
     3841 gttaacaatc ttgagcacaa taaactgtta gcacgtacta ttcagtatat tggtggcgat
     3901 ggcaaactta cagagacgat tgctaactat agtcaacagt tattaaagca atttcttact
     3961 gttttgacaa atattttaac atctgtcacg gttattgctt ctgcaattat taatcttttt
     4021 attagttttg ttttttcgtt atacgtttta gcaagtaaag aagatctttg tcgtcaggga
     4081 aatactttag tagatactta tactggtaag tacgctaaac gcattcatta cttgttagag
     4141 ttgttgcatc agcgttttca cggatttttt gttagtcaga ccttagaagc tatgatttta
     4201 ggttcgctga ctgctagtgg catgtttata ttaagattac catttgcagg gactattggc
     4261 gttttagtag cttttacagc attgattcca gttataggtg cttcaattgg agcagctatt
     4321 ggatttattt taattatgac tcagtccatg tcacaggcca ttatttttat cattttttta
     4381 attattttgc agcagattga aggcaatttt atttatccga aagtagttgg tggatcgatt
     4441 ggattaccgg ctatgtgggt attaatggca attacaatag gcgcttcttt aaagggaata
     4501 gttggtatga ttattgcagt tcctttagca gcgacacttt atcaagtgat taaagataat
     4561 attcaaaaaa gacaagctat tcaaaaaaaa caagtttcct aaaaggaact tgtttttttg
     4621 tcgaataaaa agatggaggt aatatgataa atgtattcta taaaatgtga tgataataaa
     4681 gccatgccaa gagaacgttt gatgcgacta ggagcagagt ctctaagtaa tcaagaatta
     4741 ttagcaattt tattacgaac aggtaataaa gaaaagcatg tcttagagct gtcatcctat
     4801 cttttatcgc atttagacag tctggcagat tttaaaaaga tgtctttgca agaattacaa
     4861 catttggcag gtataggaaa agttaaagcg attgaaatta aagctatgat tgagttggtt
     4921 tcccgaattt tagcgacaga taagacatta actgatagcg tattaaccag tgttcaggtc
     4981 gctgaaaaaa tgatggcagc tttaggagat aaaaaacaag agcatttagt cgtattgtat
     5041 ttagataatc aaaatcgtat tattgaagaa aaaactattt ttattgggac tgtccgacgt
     5101 tcacttgcag aaccaagaga aattttatac tatgcctgta aaaatatggc gactagtctc
     5161 attgttattc ataatcatcc ttcaggaaat attgaaccta gttctaacga ttattgcttt
     5221 actgaaaaaa taaaacgatc atgtgaagat ttaggcatta tctgtctaga tcacattatc
     5281 gttagctata aagattatta tagttttcga gaaaaatcaa ccctttttta aatcagaagt
     5341 cattgacgaa taagcggaac aattgtagat cttcatctcg aacaccttgg agaagttcag
     5401 gatgccactg aacacctaaa aaaggaatgg catcgttggt tgatatgact gcttcgatgg
     5461 taccatcacg tggatctcta gcgattactt ttaaatcctt agcaacagtt tttaaacttt
     5521 ggcgatgaaa agaattaatt aatgtcttat gaccgtaaat aggatataag atagagtctg
     5581 gctcaataat catttcgtgt gataagaaat cagaaggcgc ttcttgccaa tgtgagtcaa
     5641 tgtgttggtt aagatttcct cctaaggcaa cattcattaa ctgtgtacca cgacaaatcc
     5701 ctaagattgg tttttttaat gttatagcct ccttgatgat agctagctca aatgtgtctc
     5761 gctctggtga aaaatcatca tcaaaagcag ccttctcttc ttgataatat tttgggtcaa
     5821 cgttttgccc accaattaaa atgattttat caaccataga tacataagtt ttagcggcgg
     5881 cttcatcacc aatgggtaaa agaagcggca gccccccaga ttgagtgact gcctgtacaa
     5941 atccagtagg tgcatatgac catgggagat tatccaaagc catatttagt ctttgatttg
     6001 cagttattcc aataatcggt ttggtcatta tacctcctta atctagatga ttttgattca
     6061 taaaatagag tagtgtctgg agctcactgg tcaaatcgac agattgaaca ataactcctt
     6121 taggaacttg taggtgaaca ggagcaaaac tcaagattcc tttgattcct acttcaataa
     6181 gttgatcagt aacttcttga gcatgaatgc tgggaaccgt taagatagcc gtttcaatgt
     6241 cagtgtttgc aatccgttct ttgacggatg agataccatg gactggaata ttatcagctg
     6301 ttttagtacc aacaagagcg ttatcatcgg tatcaaaacc catggcaatt tgcatcttat
     6361 tacgatcatg gaagcggtaa tgtaatagag cacgccctat attaccgcat ccaactaaga
     6421 taacgtttgt tgtagagtga tcattaagca agtcagcaaa aaaattcatc aatttggtaa
     6481 catcatagcc aaacccacga cgccccaatt ctccaaaata agaaaagtca cggcgtacag
     6541 tagcagagtc aattcccatt gcatctgcaa tttgtttaga actggctttt tcaacttggt
     6601 cagcatggaa tcgtttaaaa atacggtaat aaagtgataa gcgctttgca gtcgcttttg
     6661 gaatagattt gtcaataacc actattttac tccttgtaga tttctcgtta aatatgtctg
     6721 tctttattgt aacaaaaaga ttgtgaaaaa tgcaacattt tgattgtttt tgtatgagtt
     6781 aaaaaagcta aaatt
//
LOCUS       DAUGHY010000061         6626 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.061, whole genome shotgun sequence.
ACCESSION   DAUGHY010000061 DAUGHY010000000
VERSION     DAUGHY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.061"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..1001
                     /locus_tag="VH752_001490"
     CDS             <1..1001
                     /locus_tag="VH752_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ8289832.1"
                     /translation="ELILVKPKLQSSHLRSVNTLTISKIVYQKKFFHLAKKSEKVIST
                     YHVTDDLKPFQVKDLVSGHLERIQEEVEKKYPNAGFNSDKYNGLKESNSLLSDGFEVK
                     SGNLIFDKKLTIPLTTLFDVINPDFLANSDRAAYDNYRTYKEQHPKKLVALTFDDGPD
                     PTTTPQVLDILAKYQAKGTFFMIGSKVVNNENLTKRVSDAGHEIANHTWDHPNLTNLS
                     VSEIQRQVNMTNQAIEKACGKKPRYLRPPYGATNATVQQSSGLTQMLWTVDTRDWENH
                     STDGIMTNVKNQLQPGGVVLMHDIHQTTINALPTVMEYLKAEGYECVTVSELYAHQ"
     gene            complement(1112..2197)
                     /locus_tag="VH752_001491"
     CDS             complement(1112..2197)
                     /locus_tag="VH752_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ8289833.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVDQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFANIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDIDNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
     gene            2295..2921
                     /gene="udk"
                     /locus_tag="VH752_001492"
     CDS             2295..2921
                     /gene="udk"
                     /locus_tag="VH752_001492"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEQ8289834.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            3001..3264
                     /locus_tag="VH752_001493"
     CDS             3001..3264
                     /locus_tag="VH752_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEQ8289835.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERNLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITWYTSILLTLLVTYIFMK"
     gene            complement(3317..4126)
                     /locus_tag="VH752_001494"
     CDS             complement(3317..4126)
                     /locus_tag="VH752_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEQ8289836.1"
                     /translation="MKQITQQKWLRYGLFIALVLNGIELELLGLTANNLSFKEAFALI
                     LTISLLGIYLIPFAAAIFYLSKKFHMNLNVIIVSCLSGLYISGFLASCGNHLVGQFWS
                     YIIPSKDALKLWGDALTAPIVEEPIKASSAILVITLFPRLTLKEKLVVALLSGMGFQL
                     TEDIRYLIQAKSIDSLVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQI
                     FWLLSPLVLHFIWNSPLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
     gene            4271..4768
                     /locus_tag="VH752_001495"
     CDS             4271..4768
                     /locus_tag="VH752_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEQ8289837.1"
                     /translation="MNKSKKIEQYQLMIEQAKELFANESNALANLSNASALLNMTLPN
                     SVFAGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMTEIVVPMVKEGHLIGVLDLDSSLVADYDEVDQEYLEAFVDLFLEKTTFTFNM
                     FGVKN"
     gene            4768..6453
                     /gene="dnaX"
                     /locus_tag="VH752_001496"
     CDS             4768..6453
                     /gene="dnaX"
                     /locus_tag="VH752_001496"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEQ8289838.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGLAYEVDALNLIARRAEGGMRDALS
                     ILDQALSLSPDNQVTIAIAEEITGSISILALGDYVRYVSQEQATQALAALETIYDSGK
                     SMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMITVVTSHLPEIK
                     KGTHPRIYAEMMTIQLAKEEGVALPSGLSSDVTTEVEGLKRELNQLKQALAQLQASPS
                     PSHAPVKPAQAKPKLTTYKIDRVKILKIMEETVQDSQQSRQYLDALKTAWNEILDSIS
                     AQDRALLMGSEPVLANSENAILAFEAAFNAEQAMKRTDLNAIFGNIMSKAAGFSPNIL
                     AVPKADFHHIRSEFALQLKTQKASDPTEETIEETLEIPEGFDFLVDKINAVED"
BASE COUNT         2148 a         1119 c         1345 g         2014 t
ORIGIN      
        1 aagaattgat tttagtaaag cctaaactgc aatcttctca cctaagatca gttaacactt
       61 tgactatttc taaaatagtt tatcagaaaa aattttttca tttggctaaa aaatcagaaa
      121 aagttataag tacatatcac gttacagacg atttgaaacc gtttcaggta aaggatctag
      181 tatcaggaca tttagaaaga atacaagaag aagttgaaaa aaaatatcca aatgctggtt
      241 ttaatagcga taagtacaat ggcttaaaag aatctaattc tttattaagc gatggctttg
      301 aggtaaaatc gggaaacctt atttttgata aaaagctaac gatacctttg acgacattat
      361 ttgatgttat taatccagat tttttagcaa atagcgatag agctgcgtat gataattata
      421 ggacctacaa agaacagcat cccaaaaaac tagttgcatt aacgtttgat gatggtccag
      481 atccgacgac gactcctcaa gttttagata ttttggcaaa ataccaggct aagggaactt
      541 tctttatgat aggttcaaaa gttgtgaata atgaaaacct tactaaacgt gttagcgacg
      601 ctggccatga aattgctaat catacttggg atcatcccaa tctgactaat ctttcagtca
      661 gcgagattca acgtcaagtt aatatgacga accaggctat tgaaaaagct tgtggcaaga
      721 aacctcgcta tttacgccct ccatacggag ctacaaatgc aactgttcag caatcctcag
      781 gattaacaca aatgctttgg acggttgata caagagattg ggaaaatcat agcactgatg
      841 gcattatgac taatgtcaaa aaccaattgc aaccgggagg agttgtttta atgcatgata
      901 ttcatcaaac aactatcaat gctttgccga cagttatgga atatctaaaa gcagaaggat
      961 acgaatgtgt gactgtatca gaactctatg cgcatcagta agtaatatcg ttatgtttct
     1021 ttagaattga tatactaaac aatccaatta cttcaaaagt aattggattg ttgtttttag
     1081 tagcccttga ttaagttttg ttttttaaca actagttgat gaaatgaagt tgctgatttt
     1141 tgagggagat ttcagagact ttagcaaatt ttttcaattt tttgaggtct tcagggtggc
     1201 taacaaaggt aataacgata ccagattttc ccatacggcc ggttcgtcca gctctgtgag
     1261 tgtaattttc cttatcacga gcaacatcaa aatgaatgac atagtcaaga ttatcaatat
     1321 caatgccccg agcaacaaga tccgtagcta acaataagga gagttggtgg gatttgaatt
     1381 tttcgagaat ggttttacgg aatttgacat taatgtcact cgctaaggaa accgcagcag
     1441 ctccattata ctggagacgt tcttccgtag cacccaaatc agataaactg ttgaaaaaga
     1501 ccaaagctct aaaatgagga atattagcaa attttcgtaa taaatcggta cgttcgcgct
     1561 tgtcaaccat gaggtaaaaa tgctgaatag catcgttggt ttgctctgaa agatcgatgc
     1621 agaatgtatt tggagctaag gaagtttgat ccaccttatt agtagcactc atgtatacca
     1681 tttgatgatc tcgaggaaca tagtggctaa ttttttgaac aaaatcatat tgggaatcac
     1741 ctaataattc gtcatattca tctaaaacaa tggtgttgac agacatcatc ttgatttttt
     1801 ttaatttaat cagttcaaaa atgcgtccag gggttccaat caggatttca ggaccttttt
     1861 tgaggcgttc gatttgacgt ttttgactgg ttccagaaat aaagagttgt gccgttaagc
     1921 ctagtggctg tgcccaatcc tttgtgactt caaaaatttg gccagctaat tcggtattag
     1981 gggctaaaat gagtagttgt tgggactttt taggcgtcag tgccaataga ctaggaaaga
     2041 cataagctaa ggtcttgccc gtcccagtag ggctaatacc aaggaaattt ttaccatcaa
     2101 caataggttg aaaggcctgt tcttggattg gcgttaaatg ggtaaatgca acttggtcaa
     2161 gtttttcttg ccattgaggg ggaaatttag tgatcatgcg tcttcctttt actattgttt
     2221 ctatattcat tatagcatta tagtctcaat tttggtaaaa tagtgagaaa gactttttag
     2281 atttggagtt taaaatgctt aaaaaaccga ttattattgg tgtaactggt ggttcaggtg
     2341 gcggtaaaac aagtgtgtca cgcgctattt tggacagttt tccaaatgct cgtattgcga
     2401 tgattcaaca cgattcttac tataaagatc agtctcacat gagttttgaa gaaagggtca
     2461 aaaccaatta tgaccatcct ctagcttttg atacagattt tatgattcag caactaaaag
     2521 aattattagc tggtagacct gtagatattc ctatctatga ttacaaaaag catacccgca
     2581 gtaatacgac ctttcgtcag gatcctcagg atgttattat tgttgaaggt attcttgtcc
     2641 tagaagatga gcgtttgcga gatttgatgg atatcaagct ctttgtagac acagatgacg
     2701 atattcgtat tattcgtcgt attaagcgtg acatgatgga acgtggcaga agcttagaaa
     2761 gtattattga tcagtacaca agcgtggtaa agcctatgta tcatcagttt attgagccaa
     2821 gtaaacgcta tgctgatatt gttattccag agggagttag taatgtcgtt gctattgatg
     2881 ttattaatag taaaattgcg agtattcttg gagaagtata gtggaagtgg ctgagtagat
     2941 tatttaaatc tgctctttta ttgttgtgct cttcctagag aaagggatca taaaatagaa
     3001 atgttattaa tttggcttgg attactattt gtttggaatg aactcgtgtt tgtcatatat
     3061 gcctttgata aaagaaaagc cataaaaaag aaacggcgta ttagtgaaag aaacttatta
     3121 gtgataacag ttttgtttgg aggttttgga gcattacttg cagctaaaaa gtaccaccat
     3181 aaaaccagaa agtggtactt tgtcatcact tggtatacta gtattttgtt gacattacta
     3241 gtaacttata tctttatgaa gtgagtcact taaaagaccc ttaatcagta ttatttagtc
     3301 aaggctcttt tatagtttaa aaaagcacat catcgtcaag ggtgttgatt ttttgaaaaa
     3361 gatcaccaaa gattaaaagg attaatgttc ctagtaaaac tgtcatgcca ggaatgcttg
     3421 ttagaggaga gttccagatg aaatgtagaa ccagaggact taataaccag aaaatttgtt
     3481 gctgttttga aaaaaggtta gaacccttta ataataagta tgctccaatg gtaaaaatag
     3541 ctgtatgtac ccaatgtgaa gtcacagcag tgctaatacg ttcaatagca gtcggtacga
     3601 gactatctat agattttgct tgaatcaaat aacggatatc ttctgttagt tgaaagccca
     3661 tgccagaaag taaagcaaca actaattttt ctttaagagt aagtctagga aataaagtaa
     3721 ttactaaaat agccgaagaa gccttaatgg gctcttcgac aataggggca gttaaagcat
     3781 ctccccaaag ctttaaagcg tctttagaag ggatgatgta tgaccaaaat tgtcctacga
     3841 gatggttccc acaagaagct aaaaaacctg aaatataaag tccgctaaga cagctaacaa
     3901 taataacgtt caggttcata tgaaattttt tacttaaata aaaaatagct gctgcaaatg
     3961 gaattaagta aatgcctaat agactaatgg ttagtattag agcaaaagct tctttaaagg
     4021 aaaggttgtt agctgtcagt cctaacaatt caagttcaat accatttaaa actaaagcaa
     4081 taaaaagacc gtatcgtaac catttctgtt gagtgatttg tttcacgaaa acctcctaat
     4141 attaaggtga tattaataat attgaaaatt atatcataaa caacctcaaa aaccatttta
     4201 aaaactgact gaagcgaaag atagtcgtaa aaaatactcg aatagaccca gatatgctat
     4261 aataaaaggt atgaataagt ctaaaaaaat tgagcaatac caactaatga tagagcaggc
     4321 aaaagagctg tttgcgaatg aatcaaatgc tttggctaat ttgtcaaatg ccagcgccct
     4381 gttaaacatg acactaccca attctgtttt tgcgggattt tacctttttg atgggcagga
     4441 attaattctt ggcccttttc aaggtagggt ttcttgcgtc catatcaagc ttggtaaagg
     4501 tgtttgtggc gaatcagctc agtctcgtcg aaccattatt ataaatgatg tgaagcagca
     4561 tgccaactat atttcctgtg atgcggcagc catgactgaa attgttgtgc ctatggtgaa
     4621 agaaggccat ttgattgggg tgctggactt ggattctagc cttgtagctg attatgatga
     4681 ggttgatcaa gaatacttag aggcctttgt ggacctattt cttgaaaaga ccacgtttac
     4741 ctttaacatg tttggagtta aaaactgatg tatcaagctc tttatcggaa ataccggagc
     4801 caaacgtttg acgaaatggt gggacaatcg gttatttcca caactttaaa gcaggcagtt
     4861 gaatctggca agattagcca tgcttattta ttttcaggtc ctagagggac tgggaaaaca
     4921 agtgcggcaa agatttttgc aaaggccatg aattgtccta accaagtcga tggtgaaccc
     4981 tgtaatcaat gcgatatttg ccgagatatc acgaatggaa gcttagaaga tgtgattgaa
     5041 attgatgctg cctcaaataa tggtgttgat gaaattcgtg acattcgaga caaatcaacc
     5101 tatgcgccaa gtcgtgcgac ttacaaggtt tatattattg atgaggttca catgttatca
     5161 acaggggctt ttaatgcgct tttgaaaact ttggaagaac cgacagaaaa tgttgtcttt
     5221 atcttggcaa caacggaatt gcataaaatt ccagccacta ttttatctcg tgtgcaacgc
     5281 tttgaattca aagctattaa gcaaaaagct attcgagagc atttagcctg ggttttggac
     5341 aaagaaggtc ttgcctatga ggtggatgct ttaaatctca ttgcaaggcg agcagaagga
     5401 ggcatgcgtg atgctttatc tattttagat caggctttga gcttgtcacc agataatcag
     5461 gtcaccattg caattgccga agaaattaca ggttctattt ccatacttgc tctgggtgac
     5521 tatgttcgat atgtctccca agaacaggct acgcaagctc tggcagcctt agagaccatt
     5581 tatgatagtg ggaagagcat gagccgcttt gcgacagatt tattgaccta tctgcgtgat
     5641 ttattggtgg ttaaagctgg cggcgacaat caacgtcagt cagctgtttt tgataccaat
     5701 ttgtctctct cgatagatcg tatattccaa atgataacag ttgttactag tcatctccct
     5761 gaaatcaaaa agggaaccca tcctcggatt tatgccgaaa tgatgactat tcagctagct
     5821 aaagaagaag gagtagcgtt accatcaggt ttgtcatcag atgtgacgac tgaagttgag
     5881 ggacttaaac gtgaacttaa tcagcttaag caagccttgg cacaattgca agccagtccc
     5941 agtccctcac atgctcctgt aaaaccagca caggctaaac cgaaactaac cacttacaag
     6001 attgatcggg tgaaaatact gaaaatcatg gaagaaacgg ttcaagatag tcagcaatca
     6061 cgccaatact tggatgccct aaaaacagct tggaatgaaa ttttagacag tatctcagct
     6121 caagatagag ccttactaat gggatcagaa cctgttctgg ccaatagtga gaatgctatt
     6181 ttagcttttg aagctgcttt taatgctgag caagcaatga agcgtactga tttgaatgcg
     6241 atattcggca acattatgag caaagcagca ggcttctcac ctaatatttt ggcagtccct
     6301 aaagctgatt ttcatcacat tcgttctgag tttgccttac aattgaaaac tcaaaaggct
     6361 tctgacccta cagaagagac gatagaagaa acccttgaga ttccagaagg ctttgatttt
     6421 ctagttgata aaattaatgc cgttgaggat taaacattat tagctgttaa tgaaccagta
     6481 acgctataaa ggtaagccta ttgggcttac ctttagcgtt tttttatcta atataccaag
     6541 atagtttttc tagccaatcc tctaatagta gaagtgaaac caaaaaagat tgatgctttc
     6601 ttgggtatcc aagtcagcat caatct
//
LOCUS       DAUGHY010000062         6491 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.062, whole genome shotgun sequence.
ACCESSION   DAUGHY010000062 DAUGHY010000000
VERSION     DAUGHY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.062"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            119..280
                     /gene="sagA"
                     /locus_tag="VH752_001497"
     CDS             119..280
                     /gene="sagA"
                     /locus_tag="VH752_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HEQ8289839.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            502..1452
                     /gene="sagB"
                     /locus_tag="VH752_001498"
     CDS             502..1452
                     /gene="sagB"
                     /locus_tag="VH752_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HEQ8289840.1"
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEEN
                     VRDFAEYGAINAENCNIIIIYVYHYIKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            1449..2507
                     /locus_tag="VH752_001499"
     CDS             1449..2507
                     /locus_tag="VH752_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HEQ8289841.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAIDTETYENTLEPETFANLMEVISGLYYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNDPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     TDVSSRLDALEHRRNMNCLSEALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQILPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            2527..3885
                     /locus_tag="VH752_001500"
     CDS             2527..3885
                     /locus_tag="VH752_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEQ8289842.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            3860..4531
                     /locus_tag="VH752_001501"
     CDS             3860..4531
                     /locus_tag="VH752_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ8289843.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILALVSIYVLSPQITLSLESIDSKTFFMGLVFCVLIVLIELVFLHGLRCWQKK
                     QWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWYLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKEVT"
     gene            4528..5211
                     /locus_tag="VH752_001502"
     CDS             4528..5211
                     /locus_tag="VH752_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagF"
                     /protein_id="HEQ8289844.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLVALSYQDFLDV
                     LLSLFQFVIIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIVILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVT
                     YLMISCKEY"
     gene            5234..6157
                     /locus_tag="VH752_001503"
     CDS             5234..6157
                     /locus_tag="VH752_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289845.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            6166..>6491
                     /locus_tag="VH752_001504"
     CDS             6166..>6491
                     /locus_tag="VH752_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8289846.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGAL
                     TAVLEV"
BASE COUNT         2024 a          999 c         1261 g         2207 t
ORIGIN      
        1 acaacagtac aattgagcta gccttgtcct tgttgtgtta actttatttt taaaataagg
       61 ttaaaaataa acgactcgtg ttcttatcag ttacttatta gataaggagg taaaccttat
      121 gttaaaattt acttcaaata ttttagctac tagtgtagct gaaacaactc aagttgctcc
      181 tggaggctgc tgttgctgct gtactacttg ttgcttctca attgctactg gaagtggtaa
      241 ttctcaaggt ggtagcggaa gttatacgcc aggtaaataa tctatttagc atctctatgt
      301 gatagtgata ttaaggtaat gagtttgcta gcaactaagt tatctttggt aacttctgat
      361 tatagatgaa tggcatagag gtgttagaaa acatgagaca aaagtaatct tcttgatgct
      421 aacgtctcag gtaattagca ggtactagac agtacctgct aattactata tgtttagtaa
      481 aatgagatag gaaagatagt tatgtcattt tttacaaagg aacaacaacc taaagagaat
      541 tgtccgccaa taactgttga aaaagcaagg caattgtttg aatttaatac aaaccacttg
      601 tccttatcgg attaccatca tcaaacggtg ctaaaaacat caaagcagct agttgctcaa
      661 catttaatgc ctaatgcaac cgataatctt agtcaacatt ttttgatgaa ctataaagct
      721 aataataatt atttaggctt ccaagctagt attgtcgact tttttacaga ttctgccgtt
      781 gctaattttt caagtagtta cgtttatgaa agtcaggaaa agataattcg tttaccaaaa
      841 cctaccaaga tatcaactgc tctgtcgaca tgtattataa aacgaagaag tcatcgtcaa
      901 ttttcagata gacaaatgcc tcttcaagat ttatcaaaca ttctttatta tgcatgcggt
      961 gttagttcac aagcatcaat tagagatgga gcatcagata agattacact cagaaactgt
     1021 gcttcaggtg gaggtttata ccctattcat ttagtttttt atgctagaaa catcagtaaa
     1081 ttaatagatg gtttctatga atatctaccc tatcagcatg cactaaggtg ttatcggcat
     1141 agctctgagg aaaacgttag agattttgcg gaatacggtg ctattaatgc tgaaaattgt
     1201 aatattatta ttatttatgt ctaccattac ataaaaaata cacgtaaata tgggaatcag
     1261 gcgactgcct atgcttttat tgaatcagga gaaatagccc agaatattca attgactgca
     1321 actgccttaa cttatggaag tattgatatt ggtggttata ataaggaata tctccaagaa
     1381 ttattagatt tagatgggct aggagagcat gtgattcaca tgacactcgt aggaactaag
     1441 gagtctcaat gaaatatcaa cttaatagta atgttcgtgt cgtgacattt caagatacat
     1501 tttgttttag aaaaggaatc tgggacttta atgaagccat tctagatttg acacaagaac
     1561 ctcaaaattt aaagatagtt tatcaagaaa tagtttcaca gttggttaag ggagtagcca
     1621 ttgatactga aacttatgaa aatactttag aacctgagac gttcgctaat ttaatggaag
     1681 tcatttcagg cttgtattac aatgatatgc tgatgcttga ggatgactac aatcttgaag
     1741 aaaatgtcat gaaaatttta atgggtaatt tccgttttat ggcgcaagca ggtcatatgg
     1801 taagcaatga tcctgttttg tttatcagtg attcgactta tgttaatgaa tctgctgagt
     1861 tgctagctga acaactgcat ttacaattgc aagtggctag cgatgactta aaaatgttaa
     1921 ttcagcagac agatgttagt tcgcgtttag atgctttgga gcatcgccgt aacatgaatt
     1981 gtttatcaga agccttgtgc gattaccaaa gtattattat ttgtcaagaa cgcttgaata
     2041 ttatgatgtt gcgacacttg aatgaagtta gcgtagctat gaaaaaacaa ttagtgattg
     2101 gttttgtaga tggaccattc ttgcacactt gtactttgaa tcctcctcat agtgctgact
     2161 ttgatagctt agaaagaaga gttttggcac gtttacaaga ctcaactctc tatcaacatt
     2221 ttgctaatca gattttacca gcaacacaag atgtgagcca ggcctattta ccattgttaa
     2281 atgttttaat gaatttagtc gttagcgaag ctttcattat tgctcaaaca ggtagttcta
     2341 aatttgaggg acgtttatta agcatttact taccgacgct tgagattcag gtccaagata
     2401 ttttgaaaat gtctaattca cagacacaag gagctttggc aaaattaaaa tatgaggatc
     2461 agcagatatc aacacgtgag attgttaaaa aactccttga cgagtaacct ataaggagaa
     2521 tctattatgt tatactatta tccttctttt aatcatattt ttgatgaatt aaaaagttta
     2581 agtggcaatc gaacaggtat cttaaatcaa tctcaagttc cagtttgcaa ccatcctcac
     2641 gatgtttact taaaaagtat tactggccaa atgccagatt atcataaaca atttataggt
     2701 gagttgagtc aagttagtta tcacattatt ggttacggca gttactatga agaagcactt
     2761 atcaagtatt taggagaaag tattgaacgc tatgctactg taattgccgg agacttacta
     2821 tcagatcgga ttgtctatgc ttcttataat gagttaaaac tgttacataa agtgatgcct
     2881 ttagaatact tgcaagtgtt tactcaagaa cagatcgcat tatcttgtga cttgcaaatg
     2941 atgatgtgcg ataaaatggt aactgagaac gatgtattgg gctgggttaa atgtcccatg
     3001 ttctttgagg acgcagagat gtatgttcca gctcaaatgc tttgtgttgg gtataaaaca
     3061 aatgagactg taggagaaag acggataata ccaggatttt ctacgggaac agcttctcac
     3121 aaaacgttag aagcggctat gtgcaacagt ttgattgaat acatccagat cgattcaatg
     3181 atgcttagct ggtatactaa gaagccttgc cccaagatta ttgttgatga tcctgatatt
     3241 gaagtgattt tagaagaagc tagactggga aaagacagtc tatacgacat tattcctatc
     3301 gatatgactg taggagaaga taatcctctt tatacgtttg gaatcattct caaaaataaa
     3361 tatgatgaag ggccttacct cttgtttgga gtgcaggctg ggttagatcc taaacatgct
     3421 ttgctaagag ggatcatgga agcttcagct atcagctata gttactacta taatctttta
     3481 tatcaaaaag cttcccttgc aaatattgaa tgcgaagaac ctttgttttt agatttagat
     3541 agtaatgttt tctattatgc acatcccaag gaccaagacc ataaatggaa ggcctttgaa
     3601 cctttgattt caggagaagt tctgttaagt gatttggaag atcattctgg taaagataaa
     3661 aaagaagatt tgaagacttt attagcttat gctaagaaag tcagtcctaa tgctgtcttt
     3721 ttagatatta cacctcctga agctctagaa aaaggctggt acgtcacgcg agttctgatg
     3781 ccagaacttc ttgaaatgtg tataccagca tttccttttg ctaatcaccc acgaatgaga
     3841 cagtttggag gtgtaacaaa tgcctttgtc catccaatgc cttaatcttt gttttttgtt
     3901 ggtaactttt tgtccttcaa ttcctatgca agctattttt ggaaaagaag atagtggtta
     3961 tgcatttaat ttaattggtt ttctaagagc aactctgatt tatgacattt tggctttggt
     4021 aagtatttat gttttgtcac ctcaaataac cttgtctctt gaaagtattg atagtaagac
     4081 gttctttatg ggacttgtat tttgcgtctt gatagtactg attgaactcg ttttcttaca
     4141 tggtttacgt tgttggcaaa aaaagcaatg gcttcctgca actttctcgt ttgtaggaac
     4201 gacaaatgat tggtctaaga ttggctatcc tttattacta gctttatttg aagagacgat
     4261 ttatcgtttt ttgtggttta atatattagc ttttcaatgg tatttaccaa caattattgt
     4321 tttaattgta actagttttt gctatgcttt aaatcactta ttgatgggga aatccatttt
     4381 ttatgctaag ttggtaacag gcatcattta tggtagtatt tacatgctga ctagtcaatt
     4441 gtggttggtt gtgataatgc atgtaggagg gaacttgcta gttgagtgtc ttagccatct
     4501 tcaaacaaaa aagaagaagg aggtgacatg atgctattgg ttttgctgtc gtttttaggt
     4561 ctaactactc tttggttgac cagttatcga agacgttgtt tggctagatg gcatcttcaa
     4621 tggttagtgg ccttatccta tcaagatttt ttagatgtct tactgagtct gtttcaattt
     4681 gtcattatta tacttgtttt attcttttac tcagctacta ttaatctagg agaggttctg
     4741 acctttttga cacaaactag ctggcattgg cagattcttt gttatttagt gttgtatctt
     4801 atggccatca tagagatgac gcttttagtt ttaattctta tctttgatgt tttgctccag
     4861 aaagatagtc gattgctctt taaaaaaata acttggctgc cttttagaca agataaacca
     4921 gtcttaagta tacttttgct aggcatagtt attctagtag acagtctctt ttatttgggc
     4981 ttgcttatat tattaggaga acagtcgcta acaagtttag caacgcttat tttaggctat
     5041 gggatagtca aggcttgtcg ctattcaggt tggttaaatg tactattagc tttttgtctt
     5101 ttcactatag ttggactatg ggcagttacg gcaactttat tatatggttg gctagtaggt
     5161 gcgatgatat taacggtgac ttatttgatg attagttgca aagagtatta gcaatattaa
     5221 aaaggagtga tatatgagtt ttgtacaatt aacaaatgtt gtcaagtcct acaaaaacgg
     5281 caagaaagct gtcaatgatg tttccttgtc tattgaagca ggtaatattt atggtttgtt
     5341 aggaccaaat ggtgctggta agtctacctt gattaatctt atcttaggct tgatcccttt
     5401 gagttctggt aaaattactg ttttagggca atcccaaaag actattcgaa aaataagttc
     5461 gcagataggt tatgttcctc aagacattgc tgtttatcca gacctaactg cttatgaaaa
     5521 tgtagaacta tttgggtcac tttatggctt aaagggagct cagcttaaaa aacaagttct
     5581 aaaaagttta gaatttgtgg ggctacactc ccaagctaag cagtttccaa gtcaattctc
     5641 aggaggaatg aagagacggt taaatatcgc ttgtgcgcta gttcattcac ctaaattaat
     5701 catttttgac gaaccgactg tagggattga tcctcaatca cgtaatcata ttttagagtc
     5761 gattcgtttg ctaaacaaag aaggtgccac agttatttat acgacccatt atatggaaga
     5821 agtagaggct ctttgtgatt atatttttat tatggatcat ggtcaagtta ttgaagaagg
     5881 acctaaattt gaactggaaa aacgttacgt tgcaaatcta gcaaaccaga ttattgtaac
     5941 tctaacagat tcacgtcatt tggaactggc agataagcct gattggtctt tgatagaaga
     6001 tggagaaaaa ctcatgttga agattgataa tagtgatatg acatcagttg ttcatcagct
     6061 cacacaggcc aatattactt ttagcgagat tagacataac catttgaatt tagaagaaat
     6121 tttcttacac ttaacaggta agaagttacg agattagagg ttactatggt tttatttcat
     6181 ttaatcaaaa aagaaagttt acagattttt agaaaccgaa cagccttatt aatgatggtg
     6241 atttttccaa ttttgatgat cgttatttta agttttgcct ttaaatcaag ttttaatact
     6301 gcgacaacag tccctaaatt gaccattcgt taccaattag agggtgaaaa aacggattac
     6361 cagaaaaatt ttcttgcttt tttaaaagtt ttaaaccaaa aacttcattt agaggctaaa
     6421 cctagtaatt ctcttgaaaa agatcgacaa agggtcagtg aaggagcctt aacggctgtt
     6481 ttagaagtga a
//
LOCUS       DAUGHY010000063         6098 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.063, whole genome shotgun sequence.
ACCESSION   DAUGHY010000063 DAUGHY010000000
VERSION     DAUGHY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.063"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(121..2112)
                     /gene="uvrB"
                     /locus_tag="VH752_001505"
     CDS             complement(121..2112)
                     /gene="uvrB"
                     /locus_tag="VH752_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEQ8289847.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRPSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
     gene            2305..4479
                     /locus_tag="VH752_001506"
     CDS             2305..4479
                     /locus_tag="VH752_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ8289848.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGSFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPIKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATITDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLLAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTVFVDVVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
     gene            4479..5219
                     /locus_tag="VH752_001507"
     CDS             4479..5219
                     /locus_tag="VH752_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289849.1"
                     /translation="MTELKIDVQGLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVMVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKESKEVAKKHGMALLEKVGLSDKADAFPGSLSGGQKQRVAIARSLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQF
                     LEDGTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            5377..>6098
                     /locus_tag="VH752_001508"
     CDS             5377..>6098
                     /locus_tag="VH752_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ8289850.1"
                     /translation="MLHKEKPDYNRNQYGFYTLDQIVPDEHFLRQVEAVIDFDFIYDL
                     VEDTYSSDNGRPSLDPVMLVKIPLIQCFYGIRSMRQTIKDIEVNTAYRWFLGLTLDNK
                     VPHFTTYGKNYSRRFQEKGLIESIFTHILGLCINTGLIDPMEIFIDGTHIKAAANNRK
                     FINREVEKQAKFMSEQLEIEINRDREKHGKKPLGPVAKKISTTDPESGWFHKGDHKEV
                     FAYSAQVACDKYGWALAYSVEAG"
BASE COUNT         1825 a         1208 c         1156 g         1909 t
ORIGIN      
        1 actaaactta ctaagttaat ctttacttta aaaagttaac ttaaaattgt cctaataaaa
       61 agaattattt gactttaact tatatatgtt aataagtata atataagctt tattcaaaac
      121 ctagtccatt aatttaagtt ctaaaatcaa gtctcgcatt tgagctgcca attcgaaatc
      181 aagtaattca gctgcctctt gcatttgttt ttgcagagca ttgatcgctt cttttcgttc
      241 tcctcttgac atactttcat aatccatctc ttcttttgat atatcattgt ggcttgtttt
      301 acttatacta ataaggcctc tgatgtcttt ttttatagtt tgtggaacga tgccgtgcgc
      361 tttgttatat gctatttgaa tctcacgacg acgagccgtt tcgtcaatag cccgctgcat
      421 agaatctgtc atcttgtccg catacataat gacatgacca tctacgtttc gagctgctct
      481 accgatggtc tgaatcaagc cgcgttcatt acggaggaaa ccctctttat cagcatccaa
      541 aatggctaca agactaactt ctggcacatc aatcccttca cggagcagat tgattccaat
      601 caagacatca aaaacaccaa gtcgtaaatc acgaataatt tctgtccgct ctaaggtttt
      661 aatatcactg tgcatatact tgaccttgac acccatctct tttagataat cagtcaaatc
      721 ttctgccatt tttttagtta aggttgtgat gaacgtccgt tcatcgcgcg ctacgcgctg
      781 gttaatttct cccaacaaat catccatttg ccccatactt ggccttacat caatttcagg
      841 atcaagtagc ccagtcggac gaataatttg ctcaataatt gtattagtct gtgacatttc
      901 gtattcacca ggcgttgctg aaacatagac aatttggtgc acatgacttt caaattcctc
      961 gcgtcttaag ggacgattat caagagctga tggcaatcga aaaccatagt caactagcat
     1021 ttgttttctg gcttggtcac cattatacat gcctttgatt tgtcccattg tcatgtgact
     1081 ttcatcaatc ataattaaaa aatcttctgg gaagaaatct aacaaggtat aaggaggttc
     1141 tcctggtgac ctgccatcca tatgacggga atagttttcg actccgctag tatagcccat
     1201 ttcacgtaac atttcaatat catattccgt tctttggcgt aatcgctgtg cttctagtag
     1261 tttaccttca gattcaaata actgtaactg ctctgctaac tctgcttgaa ttttggctat
     1321 tgattgctcc atatgctcat catttgttac aaagtgagta gctggaaata aaaccaaatg
     1381 gtcaacttct ccaatagttt tcccagttaa actttcgatt tcacatatac ggtcaatctc
     1441 atccccgaaa aattccaccc gaaaagcatg ttcatccctg gatgctggaa agacctcaac
     1501 aacatcgcca cgtacacgaa aacagccgcg ttgaaaatca atatcattac gctcaaattg
     1561 aatatctact aattgattta gcaaagtgtc acgtgatatc tcctgacctg gacgtaggct
     1621 gactgctgaa tctgcgtact cttttggcga acctagtcca taaatacaag atacagaagc
     1681 taccactatg acatcgttac gctcaagtaa agaagaggtg gctgaatggc gaagtttatc
     1741 gatttcatca ttgactgaac tatctttttc gatataggtg tcgcttgatg ggacatatgc
     1801 ttcaggttgg tagtaatcat aataagaaac aaaatattcc acagcattgt ctggaaaaaa
     1861 ttctttaaat tcaccatata attgtcctgc taaggtttta ttgtgagcta tcactaaggt
     1921 aggtttattt actttactaa ttacttggct catggtgtat gtttttcccg ttcctgtagc
     1981 acctaggaga atctgagctt tttctccgcc ctcgatatta tcaactaggc tttcaatagc
     2041 ttgtggctga tcaccagacg gtttatactt tgattttagt ttaaatggtt tatcatcacg
     2101 tttatcaatc attttatacc ctctaatagt cttactctct tatagactat tttaacatag
     2161 ataagccaga atcacctcca ataagcgtaa aaaacatgtc atttacatgt tatgttttat
     2221 gacatataaa taacaaattt cttgcttaat gttcaatatt ttgttataat taaccatatt
     2281 ttttagtttt ataaggagat tagtatgacg cacaaaataa aagtattgct gcttgcgata
     2341 atgtctattt ttttgacatg caatattgca agtgctgaaa ctattgctat tgtttcagat
     2401 acagcttatg ccccatttga atttaaagac tcagatcaaa tttacaaagg aattgacgtt
     2461 gatattatta atgaagtagc caaacgtcaa tcttgggatt tcagtatgag tttcccgggt
     2521 tttgatgcag ctgtaaatgc tgttcaatct ggtcaagcga gtgctctaat ggccggtaca
     2581 accattacga atgctcgtaa gaaagtcttt catttctcag agccatatta cgataccaaa
     2641 attgtcattg cgacacgtaa agccaatgcc atcaaaaaat acagtgactt aaaaggaaaa
     2701 acggtcggtg ttaaaaatgg aacagcggct caagcctttt tgaataacta taaaaaaaag
     2761 tatgattata ctgttaaaac atttgacaca ggtgatctta tgtataatag tttatctgct
     2821 ggttctattg ccgctgttat ggatgatgag gcggttatcc aatacgcaat cagccaaaac
     2881 caagatattg ctattaacat gaaaggagag cccattggaa gctttgggtt tgctgtcaaa
     2941 aagggaagcg gatatgatta tctagttaat gatttcaata cagctcttaa agctatgaaa
     3001 gctgatggta cctaccaagc tatcatgacc aagtggttag gcacagatga taaagctacc
     3061 accagtcagg caacgggaaa tccatctgcc aaagctacac ctataaagga cagttataaa
     3121 attgtctctg attcgtcttt tgcacctttt gaatttcaaa atggtaaggg caaatacgtt
     3181 ggtattgaca tagaattaat caaagctatt gctaaacaac aaggtttcaa aattgaaatc
     3241 gctaatccag gtttcgatgc tgccttaaat gctgtgcaat ctagccaagc agatggggtc
     3301 attgctggtg caactattac tgacgctcgt aaagctatct ttgatttttc tgatccttat
     3361 tatacttcta atatcatttt agctgttaaa gctggaaaaa acatcaagaa ctatgaagac
     3421 ttagacagaa aaacagtcgg tgctaaaaac ggcacttcat cttactcttg gttaaaagaa
     3481 aacgctccta aatatggtta taatgtcaag gcatttgatg atggttctag catgtatgat
     3541 agcttaaatt caggttctgt agatgctatc atggatgatg aggcggttct taaatacgct
     3601 atctctcaag gtcgtcgctt tgaaacacct cttgagggca tttctactgg tgaagttggt
     3661 tttgctgtca agaaaggaac taatccagaa ttaatcgaaa tgttcaacaa tggcttagct
     3721 gctctcaaaa aatctggtca gtatgatgac attatagata aataccttga ctctaagaaa
     3781 gctgcaactc cttctgaaaa aggtgctgat gagtctacta tttcaggcct attatcaaat
     3841 aactacaaac aactattggc aggacttgga accacgctca gtttaaccct tatttcattt
     3901 gctattgcta taattatcgg gatcatcttt gggatgatgg ccgtgtcacc aactaaatca
     3961 cttcgactca tttcaacggt ctttgtggac gttgttcgag ggattccttt gatgattgtg
     4021 gctgccttca ttttctgggg agtaccaaac cttatcgaga gtatgaccgg ccaccagtca
     4081 ccgattaatg atttcttagc tgctacaatt gcactgtcac ttaatggcgg agcctatatt
     4141 gctgaaattg ttcgcggtgg tatcgaagct gttccagcag ggcaaatgga agctagtcga
     4201 agtcttggtt tgtcttacgg aaccacgatg agaaaagtaa ttctcccaca agctgtgaaa
     4261 ctaatgttac ctaactttat caatcagttt gttatttcat tgaaggatac aacaatcgtc
     4321 tcagcaattg gtttagtgga actcttccaa acaggtaaaa tcattattgc tagaaattac
     4381 cagtcgttcc gcatgtatgc tattttagca attatttacc ttatcatgat tacactctta
     4441 acaagacttg caaaacgttt agaaaagagg cttaactaat gacagaatta aaaattgatg
     4501 ttcaaggttt acacaaatct tatggtcaaa atgaagtctt aaaaggaatt gatgccaaat
     4561 tctacgaagg tgatgtcgtt tgtatcattg gtccttctgg ttctgggaaa tcaacctttc
     4621 tacgtaccct caaccttcta gagacgatta ccagtgggaa ggtcatggtg gacggttttg
     4681 aattatcaga tcctaagact aatattgaca aggcacgtga aaatatcggg atggtattcc
     4741 aacacttcaa ccttttccct cacatgactg ttcttgagaa tattatcttt gctcctgttg
     4801 aattaggtaa agagtctaaa gaagttgcta aaaagcatgg catggcactt ctagaaaaag
     4861 tcggactttc tgataaggcc gacgccttcc caggaagttt atccggcggg caaaaacagc
     4921 gtgtcgccat cgccagaagt ttagcaatga acccagatat tatgctcttt gatgaaccaa
     4981 cttctgctct tgaccccgaa atggttgggg acgttttgaa cgttatgaaa gacttggcag
     5041 aacaaggcat gaccatgtta attgttactc acgaaatggg atttgctcgc caagtagcta
     5101 accgtgttat ctttacagac ggtggtcaat tcttggagga tggtactcct gaagagattt
     5161 ttgatcatcc taagcaccct cgtctcattg aatttttaga caaggtctta aacgtctaat
     5221 agagcagaca ccatactaac ttccaaaaaa taataaggca aggaaacagg tttccttgcc
     5281 tcagactgaa gacaaagtcc ttagaatcac ttttctaggg gcttttcatg atacttagga
     5341 gtataatata agaaaaagga cttgtgaggt tctcctatgt tacacaaaga aaaacctgac
     5401 tataaccgca atcagtacgg tttctatacc cttgaccaga tagtgcctga tgaacatttc
     5461 ctgcgccaag tggaagctgt gattgacttc gacttcatct atgacctagt agaagacacc
     5521 tattcgtctg ataatggtcg ccctagcctt gatcctgtca tgttggttaa aatccctctg
     5581 attcagtgct tttatggcat tcgttccatg cgtcagacca tcaaggatat tgaagtgaat
     5641 actgcctatc gctggttttt aggtttgact ttggataaca aggtgccaca tttcacaacc
     5701 tatggcaaaa attattcccg tcgctttcaa gaaaaaggct tgattgagtc tatttttacg
     5761 catattcttg ggctatgtat caatacaggc ctgattgacc cgatggagat tttcatagat
     5821 gggactcata ttaaggctgc ggctaataat cgcaagttca tcaaccgaga ggtggaaaaa
     5881 caagccaagt tcatgagtga acagctagaa atcgaaatca atcgggatag ggagaagcat
     5941 gggaaaaagc cgctagggcc cgtcgctaaa aaaatctcca caaccgatcc agaaagtggc
     6001 tggtttcaca aaggtgatca taaggaagtc ttcgcttatt ctgctcaggt ggcttgtgac
     6061 aagtatggat gggcgcttgc ttatagtgtt gaggctgg
//
LOCUS       DAUGHY010000064         6098 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.064, whole genome shotgun sequence.
ACCESSION   DAUGHY010000064 DAUGHY010000000
VERSION     DAUGHY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.064"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            42..428
                     /locus_tag="VH752_001509"
     CDS             42..428
                     /locus_tag="VH752_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEQ8289851.1"
                     /translation="MDEKKLFENFQLTFGRMISPFEIEDIQKWIHEDNMPIEVVNLAL
                     REAVENNKISWKYINKILVDWYKSGDTTVEKVRDRLQRFDDSKKQRSVTTSNVPSWSN
                     PDYKEPDLEEFALGSMDGIEDGSGDF"
     gene            409..642
                     /locus_tag="VH752_001510"
     CDS             409..642
                     /locus_tag="VH752_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289852.1"
                     /translation="MDQEIFNFFNKQIKKDFGKTASKETFAKFASYCAEGIEKNGVKP
                     IFNWINLYAFGTDITTAEADRLRIERYKQENVL"
     gene            639..779
                     /locus_tag="VH752_001511"
     CDS             639..779
                     /locus_tag="VH752_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289853.1"
                     /translation="MTKQHRETLIWYRASHQERERLLDFGLVDKARYVTLLRQLRKKY
                     AI"
     gene            788..994
                     /locus_tag="VH752_001512"
     CDS             788..994
                     /locus_tag="VH752_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289854.1"
                     /translation="MTPEQAEKAKIRAKQELETFSIYLDQAVDELGGVLTSREVFLAA
                     GFTYLGAGQTDIHAAVEGLCEQIQ"
     gene            1050..1379
                     /locus_tag="VH752_001513"
     CDS             1050..1379
                     /locus_tag="VH752_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289855.1"
                     /translation="MNIKEKIVVLRNTEDGSFLKSFKNKKDVLAYNVELTDSIQLASF
                     LPEEAYNIQKEKIDNLAETLGCDVVVIEASYDLKFIDGEDVPELTKEQKVKSMVNGMF
                     EQVFGGE"
     gene            1382..2173
                     /gene="bet"
                     /locus_tag="VH752_001514"
     CDS             1382..2173
                     /gene="bet"
                     /locus_tag="VH752_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEQ8289856.1"
                     /translation="MANQLSTQQVKRDITTDPTLLTGADIKKYFDPQNLLSEKQVGQA
                     LALCKGRNLNPFANEVYIVAYKNNSGTDFSLIVSKEAFMKRAERCEGYDGFEAGITVM
                     RNGEMVEIEGSLKLPDDVLIGGWAIVYRKDRSHRYKVTVDFNEYVKLDKYGNPRSTWK
                     SMPGTMIRKTALVQTLREAFPDELGNMYTDIDGGDTFDAIKDVTPQETQEEVRARKMA
                     QIEQYKQEQTQKQTQKADTSYPVDEVSEHTDDPVQGELLDGELEY"
     gene            2183..3211
                     /locus_tag="VH752_001515"
     CDS             2183..3211
                     /locus_tag="VH752_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEQ8289857.1"
                     /translation="MQELQLKVTQAQVEIIDREKFEQNINEVVAKYQNYAVTAGTIKD
                     DKQVLADLRKLKKQLSDERIKVKKELSKPADDIDGYIKQASKPLDDTIDKIATDVKEF
                     EDHQKALRLDTVKSYLSNKASEYMLDPRIFDEKAMEYTKAGNFMADGVTLKKVTMKSL
                     EDLVTFEYQKEQEVEKAKATVSGQCAEYGMTDQPYIRMLKEMTLVEVLGQIKADYLAE
                     KQKQEMRKAEEEREQLLAAQQTKEQEQAQKSAEIPQIDKETGEILDGWQLSQNNQETL
                     TGAENEFKKYNQKMTLEVYFEDTAEKDRFKTGLTQLGFDFKKNYQVSGYQNIEPLTQA
                     ELARRCGW"
     gene            3407..3745
                     /locus_tag="VH752_001516"
     CDS             3407..3745
                     /locus_tag="VH752_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289858.1"
                     /translation="MEQIKITETGMVVISDKALKTFVIAGHLSERWEFTSKFKKLDEP
                     SLDENGDLFEPVYELMLEARSKGQISITSSYCGKNHKKDTDEIIKVFSFIEDNKRNIF
                     ENLGIRGVLE"
     gene            3742..4254
                     /locus_tag="VH752_001517"
     CDS             3742..4254
                     /locus_tag="VH752_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289859.1"
                     /translation="MSNLVLSLDISTSGTGWALFKGSDLIQSGVLKHKSKSYFERGRY
                     MASQLGLIQSRALKKYDCYFSTIAVEKNSVMEPNQQSMLKIGIVTGIILGRLIADNVA
                     FINVSTWRKHWKFSYKDRSKKAMKAQSKEKALEYCGKTVKDDEADAILIGSYYVNQGY
                     LDELETHDYY"
     gene            4241..4435
                     /locus_tag="VH752_001518"
     CDS             4241..4435
                     /locus_tag="VH752_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289860.1"
                     /translation="MTTTKKHVVRVYNKGITATYAIYDKKLFKEHEFATKNEAMRFIR
                     QLELANDKRATEYYLREVEK"
     gene            4432..4677
                     /locus_tag="VH752_001519"
     CDS             4432..4677
                     /locus_tag="VH752_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289861.1"
                     /translation="MIPNFRGFNKKTKKMYSIDGFKSSERKIYRCSLADDEFRSGRLE
                     TFHFVEDNLVEKCNRPPERFYRQKQVRKSDRRLMARQ"
     gene            4729..5190
                     /locus_tag="VH752_001520"
     CDS             4729..5190
                     /locus_tag="VH752_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEQ8289862.1"
                     /translation="MNIEEAKELVDNSKFYGKTSSVIKAEVRDIIDQLNQPKPEVPQC
                     VADWIEECKEEDLTLKGLFSNSDMPAKIFDWIFGSDENCRLMAEAWINGYTVEKEKLY
                     TVDLPNGQPLVRGINTLYFSQNLATENVKLTESEIRKDFEWAWQFAEEVTE"
     gene            5640..6080
                     /locus_tag="VH752_001521"
     CDS             5640..6080
                     /locus_tag="VH752_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEQ8289863.1"
                     /translation="MTFFPEIDIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQPHGTPSKPVERLALNRVSAEQELDTIERAVNGIFDPEYRLILIDKYLLTYPKTD
                     CDIYTKLGYEKSQYYNMLDNALLSFSELYKEGMLLVEKLEKSWN"
BASE COUNT         2238 a         1017 c         1332 g         1511 t
ORIGIN      
        1 ctaacagata ataagcatca tcatcaacat caggaggagc tatggacgaa aaaaagcttt
       61 ttgaaaattt ccaattaact tttggacgga tgatatcgcc atttgaaatc gaagatattc
      121 aaaagtggat tcacgaagat aacatgccaa ttgaagttgt caaccttgcc ttaagagaag
      181 cggtagaaaa caacaaaatc agttggaagt atatcaataa aatcttagtt gattggtata
      241 aatctggaga tacgacagta gaaaaggtca gagacaggtt gcaacggttt gacgatagta
      301 aaaaacaacg aagtgtaact acctcaaacg tcccaagctg gtcgaatcca gactacaaag
      361 aaccagattt agaagaattt gctctaggaa gcatggacgg tatagaagat ggatcaggag
      421 atttttaatt tttttaacaa acaaatcaaa aaagattttg gtaaaacggc gagtaaagag
      481 acttttgcta agtttgctag ttactgcgct gaaggaatcg aaaaaaatgg agttaagcca
      541 atttttaatt ggataaacct atacgctttt ggaactgata taacaacagc agaagcagac
      601 cgattaagga tagagcgata taaacaggag aatgtgttat gacaaaacag catagagaaa
      661 cgcttatctg gtaccgagca agtcatcaag agcgtgagag attgcttgat tttggactag
      721 ttgataaagc acggtacgtg acactattgc ggcaattgcg taagaaatat gcgatttagg
      781 aggaaatatg acaccagaac aagcagaaaa agcaaaaatc agagctaaac aagaacttga
      841 aacgtttagc atataccttg accaggcagt tgatgaactc ggtggagttt taacttcgcg
      901 agaagtcttt ttggcagcgg gattcaccta ccttggcgca ggtcagacag atatacatgc
      961 tgcagtcgag ggattatgtg agcaaatcca atgattttaa atttagtgaa gactgggaga
     1021 gcaactaaaa cacaaaaaga ggaaatgaaa tgaacatcaa agaaaaaatt gtagtgctaa
     1081 gaaacactga agacggaagt tttttaaaga gtttcaaaaa caaaaaagat gtacttgctt
     1141 ataatgtgga acttacagat agcattcaac tggcatcatt tttaccagaa gaagcttaca
     1201 acatacaaaa agaaaaaatt gataatttgg cagaaacgct tgggtgtgat gttgtagtta
     1261 tcgaagcgtc atatgaccta aaatttattg atggcgagga tgttccagag ttaacaaaag
     1321 agcaaaaagt taaaagtatg gtaaacggaa tgtttgagca ggtttttgga ggtgaataga
     1381 gatggcaaat caattatcaa cacaacaagt taaacgtgat atcacaactg atccaacttt
     1441 gctgacagga gctgatatta aaaaatattt tgatcctcag aacttgctta gcgaaaaaca
     1501 agtagggcaa gctctggctt tgtgtaaggg gcgcaacctt aatccatttg caaacgaagt
     1561 ttatattgtg gcctataaaa ataatagcgg cacagatttc agtttgattg tatctaaaga
     1621 ggcatttatg aaacgtgctg aacgttgtga aggatatgat ggattcgagg ctggcattac
     1681 tgttatgaga aatggtgaga tggttgaaat tgagggctct ctaaaactac ctgatgatgt
     1741 tttgataggt ggatgggcca ttgtttaccg caaagaccgc tcacatcgat acaaagttac
     1801 tgttgacttt aacgaatatg tcaaacttga caaatatggt aatccacgaa gtacttggaa
     1861 atcaatgcca ggaacaatga ttagaaaaac agcacttgtt cagacactta gagaggcttt
     1921 tcctgatgaa ctcggtaata tgtacaccga tattgacggt ggcgatactt ttgatgctat
     1981 taaagatgtg actcctcagg aaacacagga agaagtcaga gcacggaaga tggcacagat
     2041 tgagcagtac aagcaagaac agactcagaa acaaactcaa aaagcagaca ctagctatcc
     2101 cgttgatgaa gtgtctgaac atactgatga tccagtacaa ggagaactac ttgatggaga
     2161 actggagtat taggaggaaa ccatgcaaga attacaatta aaagtcacac aggcacaagt
     2221 tgaaattatt gatcgtgaaa aatttgaaca aaacatcaac gaagttgtgg caaaatacca
     2281 aaattatgca gtcacagctg gaactatcaa agatgacaag caagtcctag ctgatctacg
     2341 caagctcaaa aaacagttgt ctgatgagcg tatcaaagta aaaaaggaac tctcaaagcc
     2401 tgctgacgac attgatggat atatcaaaca ggcaagcaag ccactagatg acacgattga
     2461 caagattgca actgatgtca aagaattcga agaccatcaa aaagcgctgc gattagatac
     2521 agtaaagagc tacttgtcta ataaggcatc cgagtatatg cttgaccccc gtatttttga
     2581 cgaaaaagct atggagtaca ccaaagctgg caattttatg gcggacggtg taaccctcaa
     2641 aaaagtcacc atgaaatctc ttgaggactt ggttaccttt gaatatcaaa aggagcaaga
     2701 ggtcgaaaaa gcaaaagcta ccgtctcagg acaatgtgct gagtatggta tgactgacca
     2761 accttacatc cgtatgctaa aagagatgac gcttgtagag gtgctaggtc agattaaagc
     2821 tgattatctc gctgaaaagc aaaagcagga aatgcgaaaa gccgaggagg aaagagagca
     2881 acttttagca gctcagcaaa ctaaagaaca agaacaggct cagaaatcag cagaaatacc
     2941 acaaatcgac aaggaaacag gcgaaatctt ggatggttgg caattatccc aaaacaacca
     3001 agaaacgctc acaggagctg aaaacgagtt taaaaagtac aatcaaaaaa tgacacttga
     3061 ggtgtacttt gaagatacgg ctgaaaaaga ccgtttcaag accggtctaa ctcagctagg
     3121 ttttgatttt aaaaaaaatt atcaagtcag cggttatcaa aatatagagc ctcttactca
     3181 agctgaattg gctcggcggt gcggatggta aataaaaaat agttttttaa caacctattg
     3241 caaagtgaag ctcagccttt gcagtatcaa tattttccga gtgagaaagg aaagttggaa
     3301 tatcgtcaag ttaacaggat tgatgatata aaaaattgct acactcgtcc ttgccaatgc
     3361 tcacacacaa ttttagggcg agtgtggatt ttaaaaggtg agaaatatgg aacaaatcaa
     3421 aattacagaa acaggaatgg tcgttataag cgataaagca ctcaaaactt ttgtgattgc
     3481 aggacattta tcggagaggt gggaatttac ctctaaattt aaaaaattag atgagccatc
     3541 acttgatgaa aacggagatt tgtttgagcc tgtatacgag ttgatgcttg aagccaggtc
     3601 gaagggacaa attagcataa catcatcata ttgtggcaaa aatcacaaaa aagacacaga
     3661 cgaaatcata aaagtattct cgtttatcga agataacaag agaaatattt ttgaaaacct
     3721 tggtattcgt ggggtgcttg aatgagcaat ctagttttat cgttagacat ctcaacatct
     3781 ggaacagggt gggccttatt taaaggctca gaccttatcc agagtggtgt cttaaaacat
     3841 aagagtaaat cctactttga gcgcggacgc tatatggcta gccaattagg actaatccag
     3901 tcacgagcat taaaaaaata cgattgctat tttagtacaa tcgcagtcga aaaaaattca
     3961 gttatggaac ctaaccagca atccatgctt aaaatcggta ttgttacagg aattatctta
     4021 ggaagattaa tagctgataa cgtcgccttt ataaatgtat caacgtggcg taagcactgg
     4081 aagtttagct acaaagaccg ctctaaaaaa gcgatgaaag cacaatcgaa agaaaaagct
     4141 ctcgaatatt gcggaaaaac agtaaaagat gatgaagcgg atgctatttt gattggctca
     4201 tactatgtca atcaaggcta tcttgatgaa ttggagacac atgactacta ctaaaaaaca
     4261 cgttgtgaga gtttacaaca aaggtattac agcgacttac gcgatctatg acaaaaagct
     4321 gtttaaggag cacgagttcg caaccaaaaa cgaagcgatg cggtttatta gacagctaga
     4381 gttagctaat gataagcgag cgacagaata ttatttgaga gaggtagaga aatgatacca
     4441 aattttagag ggtttaacaa aaaaactaaa aaaatgtata gcattgatgg ctttaaatca
     4501 agtgaacgca aaatatacag atgcagctta gcagatgatg agtttcgctc tggtcgctta
     4561 gagacattcc attttgtaga ggataatctt gtggaaaagt gcaacagacc acctgaacga
     4621 ttttatcgac aaaaacaagt acgcaaaagt gacaggcgac ttatggcaag gcagtgatca
     4681 agtcattgct gagcagtgtt ttttaaaagt attagaggag atgcaaaaat gaatatagaa
     4741 gaagcgaaag aattagtaga taattcaaaa ttttatggaa agactagcag tgttataaaa
     4801 gccgaggttc gcgacattat agaccagtta aaccaaccaa aaccagaagt accgcagtgt
     4861 gtggcggatt ggatagaaga gtgcaaagaa gaagatttaa ctttaaaagg cttgttttct
     4921 aacagcgata tgcctgcaaa aatttttgat tggatttttg gtagtgacga aaattgtagg
     4981 ttaatggctg aagcatggat taacggctac acagtagaaa aagaaaagct ctacactgtc
     5041 gacttaccaa acggtcaacc tttagtgcgc ggcataaaca ctttgtattt tagtcaaaat
     5101 ctagcaaccg aaaatgtaaa actcaccgaa tccgaaatcc gcaaagattt tgaatgggcg
     5161 tggcagtttg ctgaagaggt gactgaatga ctgaagagtt aggagtgtta tatagcgaaa
     5221 aatggcataa gtattattta tataaaactt gcaggtatat atcttatgtt gataatccac
     5281 atcaggctac taaatgcacc cgcaaacaag tagaacagtt tccacagttt aaatgggtat
     5341 cgctgacaaa attataaccc cacgcaagcg ctcaagagcc tgcgatggct ctgtgggggt
     5401 ggaccgaaat taaaaaatag aaacgagaac ctccttacac caaaacaaat ctaaagcggg
     5461 ttatcggtca tccgtgatta tccaaggcgt cgctaatgct ttaacacgac atcgtgcgcc
     5521 tgtgtcaaaa aacaaaggaa agagaggact tttctccaca aaacaaaaag acgtccatac
     5581 ggaacgcccc cttggttaaa tttaagctta aataaattat accacattgg ggggctttca
     5641 tgacgttttt tcctgagatt gatatccaaa aaacaaaatc caacgccaag cgtaaattga
     5701 gagagtatcc acgctggcgg aggatagcta atgatgtaga tactcaaaaa gttacagcta
     5761 cttactcctt tgagccaaga caaccgcatg gaacgccaag caagcctgtt gagagactag
     5821 cgctcaaccg tgtgtcggca gaacaggagc tggatacgat tgagagagca gtcaacggga
     5881 tatttgatcc agagtataga ttgatactga ttgacaagta tttgctcaca tatccaaaga
     5941 ctgattgtga catttataca aaacttggtt atgaaaaaag ccagtattac aacatgctag
     6001 ataatgcttt gttgtcgttc tcagagctat ataaagaggg aatgttgctt gtcgaaaaat
     6061 tggaaaaaag ctggaattaa tatggaataa ttatgtac
//
LOCUS       DAUGHY010000065         6039 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.065, whole genome shotgun sequence.
ACCESSION   DAUGHY010000065 DAUGHY010000000
VERSION     DAUGHY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.065"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            167..4273
                     /gene="cas9"
                     /locus_tag="VH752_001522"
     CDS             167..4273
                     /gene="cas9"
                     /locus_tag="VH752_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922251.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEQ8289864.1"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGEIAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLAK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMLAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVRKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PTAFKYFDTTIDRKRYTSTKEVLDATFIHQSITGLYETRIDLSQLGGD"
     gene            4273..5142
                     /gene="cas1"
                     /locus_tag="VH752_001523"
     CDS             4273..5142
                     /gene="cas1"
                     /locus_tag="VH752_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEQ8289865.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDAYKTELIHLSEIDILLLE
                     TTDIVLSTMLVKRLVDENVLVIFCDDKRLPTAMLMPFYGRHDSSLQLGKQMSWSETVK
                     SQVWTTIIAQKILNQSCYLGACSYFEKSQSIMDLYHGLENFDPSNREGHAARIYFNTL
                     FGNDFSRDLEHPINAGLDYGYTLLLSMFAREVVVSGCMTQFGLKHANQFNQFNFASDI
                     MEPFRPLVDKIVYENRNQPFPKIKRELFTLFSDTFSYNGKEMYLTNIISDYTKKVVKA
                     LNNEGKGVPEFRI"
     gene            5139..5480
                     /gene="cas2"
                     /locus_tag="VH752_001524"
     CDS             5139..5480
                     /gene="cas2"
                     /locus_tag="VH752_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEQ8289866.1"
                     /translation="MSYRYMRMILMFDMPTDTAEERKAYRKFRKFLLSEGFIMHQFSI
                     YSKLLLNNTANNAMIGRLREHNPNKGNITLLTVTEKQFARMIYLHGERNNCIANSDER
                     LVFLGEAFDES"
     gene            5470..>6039
                     /gene="csn2"
                     /locus_tag="VH752_001525"
     CDS             5470..>6039
                     /gene="csn2"
                     /locus_tag="VH752_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEQ8289867.1"
                     /translation="MNLNFSLLDEPIPLRGGTILVLEDVCVFSKIVQYCYKYEEDSEL
                     KFFDRKMKTIKESEIMLVTDILGFDVNSSTILKLIHADLESQFNEKPEVKSMIDKLVA
                     TITELIVFECLENELDLEYDEITILELIKSLGVKVETQSDTIFEKCLEILQIFKYLTK
                     KKLLIFVNSGAFLTKDEVASLQEYISLTNL"
BASE COUNT         2102 a          877 c         1196 g         1864 t
ORIGIN      
        1 ttataatcta tttattatta agtattgggt aatatttttt gaagagatat tttgaaaaag
       61 aaaaattaaa gcatattaaa ccaatttcgg aggtcattaa aactattagt gaaatcatca
      121 aactcattat ggatttaatt taaacttttt attttaggag gcaaaaatgg ataagaaata
      181 ctcaataggc ttagatatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgatta
      241 taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca gtatcaaaaa
      301 aaatcttata ggggctcttt tatttgacag tggagagata gcggaagcga ctcgtctcaa
      361 acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc tacaggagat
      421 tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg aagagtcttt
      481 tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata tagtagatga
      541 agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat tggtagattc
      601 tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga ttaagtttcg
      661 tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg acaaactatt
      721 tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta acgcaagtgg
      781 agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat tagaaaatct
      841 cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca ttgctttgtc
      901 attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg ctaaattaca
      961 gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa ttggagatca
     1021 atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac tttcagatat
     1081 cctaagatta aatagtgaaa taactaaggc tcccctatca gcttcaatga ttaaacgcta
     1141 cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac aacttccaga
     1201 aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt atattgatgg
     1261 gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa aaatggatgg
     1321 tactgaggaa ttattggcga aactaaatcg tgaagatttg ctgcgcaagc aacggacctt
     1381 tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta ttctgagaag
     1441 acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa aaatcttgac
     1501 ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt ttgcatggat
     1561 gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg tcgataaagg
     1621 tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc ttccaaatga
     1681 aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata acgaattgac
     1741 aaaggtcaaa tatgttactg agggaatgcg aaaaccagca tttctttcag gtgaacagaa
     1801 gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta agcaattaaa
     1861 agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag gagttgaaga
     1921 tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta aagataaaga
     1981 ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa cattgacctt
     2041 atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc tctttgatga
     2101 taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt tgtctcgaaa
     2161 attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt ttttgaaatc
     2221 agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt tgacatttaa
     2281 agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg aacatattgc
     2341 aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa aagttgttga
     2401 tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg aaatggcacg
     2461 tgaaaatcag acaactcaaa agggccagaa aaattcgcgt gagcgtatga aacgtattga
     2521 agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg aaaatactca
     2581 attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca tgtatgtgga
     2641 ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatcacattg ttccacaaag
     2701 tttcattaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata aaaatcgtgg
     2761 taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact attggagaca
     2821 acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga aagctgaacg
     2881 tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg ttgaaactcg
     2941 ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta aatacgatga
     3001 aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat tagtttctga
     3061 cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc atcatgccca
     3121 tgatgcgtat cttaatgccg tcgttggaac tgctttgatt aagaaatatc caaaacttga
     3181 atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatgc ttgctaagtc
     3241 tgagcaggaa ataggcaaag caaccgcaaa atatttcttt tactctaata tcatgaactt
     3301 cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc taatcgaaac
     3361 taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca cagtgcgcaa
     3421 agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga caggcggatt
     3481 ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc gtaaaaaaga
     3541 ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt cagtcctagt
     3601 ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag agttactagg
     3661 gatcacaatt atggaaagaa gttcctttga aaaaaatccg attgactttt tagaagctaa
     3721 aggatataag gaagttagaa aagacttaat cattaaacta cctaaatata gtctttttga
     3781 gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa aaggaaatga
     3841 gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt atgaaaagtt
     3901 gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc ataagcatta
     3961 tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt tagcagatgc
     4021 caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa tacgtgaaca
     4081 agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctccca ctgcttttaa
     4141 atattttgat acaacaattg atcgtaaacg atatacgtct acaaaagaag ttttagatgc
     4201 cacttttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt tgagtcagct
     4261 aggaggtgac tgatggctgg ttggcgtact gttgtggtaa atacccactc gaaattatcc
     4321 tataagaata atcatctgat ttttaaggat gcctataaaa cggagctgat ccatttatca
     4381 gaaattgata ttttgttatt agaaacgacc gatattgtct tgtccactat gctggtaaaa
     4441 cggctagtgg atgagaatgt ccttgtcata ttctgtgatg ataaacgatt accaacagct
     4501 atgctgatgc ctttttatgg tcgtcatgat tcgagtttac agcttgggaa acaaatgtcc
     4561 tggtcagaaa cagtcaaatc gcaggtttgg acgacgatta ttgctcaaaa gattttgaat
     4621 caatcttgct atctaggagc atgctcctat tttgaaaaat cccaatctat tatggattta
     4681 tatcatggtt tggaaaattt tgatccgagt aatcgagaag ggcatgcagc gagaatttat
     4741 tttaatacac tttttgggaa cgatttctca agagatttgg agcatccaat caatgcaggt
     4801 ctggattatg gttatacttt attattgagt atgtttgcgc gtgaagtggt tgtgtctgga
     4861 tgtatgactc agtttggact taaacacgct aatcagttta atcagttcaa ttttgctagc
     4921 gatattatgg aaccatttag gcctttggtg gataagattg tttatgaaaa tcgaaatcag
     4981 ccttttccca aaataaagag agagttattt actttgtttt cagatacatt ttcatataat
     5041 ggtaaagaga tgtatctcac gaatattatt agcgattata ctaaaaaagt tgtcaaagct
     5101 ctgaataatg aagggaaagg agttcctgaa tttaggatat gagttataga tatatgagaa
     5161 tgatacttat gtttgatatg ccgacggaca ccgctgagga acgaaaagcc tatcgaaaat
     5221 ttcggaaatt tttacttagt gaagggttta tcatgcatca attttctatt tatagtaagt
     5281 tgctgttgaa taatacagct aacaatgcca tgattggtcg gctgagggag cataatccta
     5341 ataaaggaaa tattacatta ctaacggtca cagaaaaaca gtttgcacga atgatttatt
     5401 tacatggtga aagaaataat tgtattgcaa actccgatga aagacttgta tttcttgggg
     5461 aggcttttga tgaatcttaa tttttcctta ctagatgaac cgattccatt aagaggcggt
     5521 acaattcttg tgctcgaaga tgtctgtgta ttttcaaaaa tagtgcaata ttgttacaaa
     5581 tatgaggaag attctgaact taaatttttt gatcgcaaga tgaaaaccat caaagaatca
     5641 gaaatcatgc ttgtaacaga tattttagga tttgatgtta actcctcaac cattttaaaa
     5701 ttgattcatg cagatttaga atctcaattt aatgagaaac ccgaagtgaa atcgatgatt
     5761 gacaaattgg ttgctacgat tacagaactg attgtctttg aatgcttaga aaatgaatta
     5821 gatttagagt atgatgaaat cacaatcctg gaattgatta agtccttagg agtaaaagta
     5881 gaaacgcaaa gtgatactat ttttgaaaaa tgtctagaga tacttcaaat tttcaaatat
     5941 ctcactaaga aaaagttgct tatttttgtc aatagcggag cttttctaac aaaggatgaa
     6001 gtggctagtt tacaagagta tatatcattg acaaattta
//
LOCUS       DAUGHY010000066         6012 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.066, whole genome shotgun sequence.
ACCESSION   DAUGHY010000066 DAUGHY010000000
VERSION     DAUGHY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.066"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(357..1229)
                     /locus_tag="VH752_001526"
     CDS             complement(357..1229)
                     /locus_tag="VH752_001526"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEQ8289868.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYISSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            1453..2763
                     /locus_tag="VH752_001527"
     CDS             1453..2763
                     /locus_tag="VH752_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEQ8289869.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KALLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPDALYMILYLIAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHSLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPILS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTLFPIIATISAQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            2841..3991
                     /locus_tag="VH752_001528"
                     /pseudo
     CDS             2841..3991
                     /locus_tag="VH752_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558257.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4118..4273
                     /locus_tag="VH752_001529"
                     /pseudo
     CDS             4118..4273
                     /locus_tag="VH752_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4320..4850)
                     /locus_tag="VH752_001530"
     CDS             complement(4320..4850)
                     /locus_tag="VH752_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEQ8289870.1"
                     /translation="MSNFISPVYEIKSIPIEKISPNDYNPNSVAPPEMKLLYDSIKSD
                     GYTMPIVCYYDKEEDRYSIVDGFHRYRIMLDYSDIYERESGRLPVSVIDKSLDYRMAS
                     TIRHNRARGSHDVDLMSQIVKDLHECGRSDNWIAKHLGMDKDEILRLKQITGLASLFK
                     DHEFNQSWEPEELLNE"
     gene            complement(4843..>6012)
                     /locus_tag="VH752_001531"
     CDS             complement(4843..>6012)
                     /locus_tag="VH752_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HEQ8289871.1"
                     /translation="EIGVFHQDFEAQYSLTTKYVQETFTSLEGRKKVSLYWVCLPMAT
                     RTALSSYEMFWYPWDDKTEDIWVRPMPSQDYVINLENNSITTYRYKMNQEDLAKQFGR
                     WYKQIHGNQKTVCILGNRASESLHRYSGFINKKYGYQKECWITKQFKDVWTASPLYDW
                     SVEDIWHAYYKFSYSYNELYDLFYKAGLKPSQMRVASPFQDYAVDSLNLYRIIDQETW
                     VKLLGRVQGVNFSNIYGRTKAMGYKSIALPKGHSWKSYTQFLLSTLPVRLRNNYVRKF
                     NKSIDFWHKTGGGLAEETINELIEKGYRIARNGISNYTSFKHSRVIFLDQIPDDTDDI
                     VTTKDIPSWKRMCFCILKNDHICRTMGFGLTREQQKIVAFLKEKYKTSLKELESE"
BASE COUNT         1848 a         1294 c          980 g         1890 t
ORIGIN      
        1 aaagcaaaac taaaaggatt tagtcctgtc caatacagaa ctaaatcctt tcaataatta
       61 tttgtccaac tttttggggt cagtacactc ctagttaacg actttgattg attttattat
      121 gatgaatcgt gatttctgaa aacaaacttt tcttcatgga tttggcgtgg aacttttaca
      181 ataaaaacac taacctctcc ctatcgtttt ttcaggtgaa tgcttggaat tggtataata
      241 agcggacacg aatcaggtga aaacagggaa agtatttgaa gaggcgttta aactatctga
      301 cataaaactc gttctagagg aaagacattc tttaaaaagt tagtttattt tgcaacttag
      361 acatcaaggt aggttaaacc cttcatggaa aaatcgagac tcttagcact atgggttaaa
      421 ctaccactgg agatgtaatc aatcgctaaa ccacgaaaac ggctaatagt ggtcatatca
      481 atatttccag aacattcaat ccgagaacgt cctgcaatta gagtaatggc ctgttcaatc
      541 tgttccaatg acatattatc caacatgata atatcaacac ccgccgccgc agcttcttcg
      601 gcagcagcaa ggctttccac ttccacctcg accattttca caaaaggggc ataggcacgc
      661 gcttgagcaa tcgccttttg aacactacct actgccgcaa tgtgattgtc ttttagcatg
      721 atagcatctg ataaattaaa gcgatgatta tagccaccgc caactctcac ggcatatttc
      781 tcaaaaagac gtaaattagg agtggttttt cgagtatcaa ataccttaat gcgatcatcg
      841 cctaaggctt ctacataagc agctgtcatc gaagcaatcc ctgataaatg ttgtaaaaaa
      901 ttcaaggcaa cgcgttcaca tgttaagagg cttctcaccg agcctatgat ttctaaaacc
      961 aaatcgccac tagtcaaacg atccccatcc ttaaattgat gaggattctg gaaggtcacc
     1021 tcggcatcaa atagggtaaa aaccctttga aaaacggtta gccccgctaa aacaccagct
     1081 tccttggcaa aaagcgacac cttggcttgg ccatgatgat caaaaatggc attggtactg
     1141 taatcttcgg aatgaacatc ttctcgcaag gctgctttca atgtatcatc tatttgaaaa
     1201 ggggttaaat cagttgaaat gattgacatc actaagtcct ctatttttaa tatcacttgt
     1261 aagcactaga ggcaattgct attaacttcc gtaggaaatc acaaacctga atacctcatt
     1321 ttaaatccca acaagtgtca agacaattgt aatttttttg tgtataatag tcaagtacca
     1381 ataggaaaca ggtggtcttt tgagcgccaa aattgccaac ctattttcat attctaacta
     1441 aaggacaatt caatgattca tttaataata attagcgcca ttgctctagc cattggaatt
     1501 ggttaccgca ccaaaatcaa tattggcctg ctggctattg ctttttctta cctcatcgca
     1561 accactctca tgggattaag ccccaaagca cttcttcatt tttggccaac ctcactcttt
     1621 tttaccattt ttagcgtctc tctcttttat aacgttgcaa caactaacgg cactcttgat
     1681 gttttggctc aacacattct ctaccgcaca cgcacccacc ctgacgccct ctacatgatt
     1741 ttatacctga tagcaaccct tttgtctgct ttaggtgctg gatttttcac tactatggcc
     1801 gtttgctgtc ctctagcgat taccctctgt caaaaagcgg acaaacactc tttgattgga
     1861 gctcaagccg tcaattgggg agcttcagga ggggctaatt tgataaccag tggctcaggc
     1921 attgtctttc aaggcctgtt taagcaaatg ggatgggaag agcaagcttt ttcacttggc
     1981 aatcatatct ttatcgtcag cattatctac ccactcatcg tcttgctttt attatcttgt
     2041 tacatccgct acagtaaggg aagaacaaac tcatccttga ctattgacca accaccaatc
     2101 ctcagcaagg tccaaagaca aaccaccttg ctcatgatca gcagcatggt tttggtttgg
     2161 ctttttccct tgttgcatct cattttccca aacatcgcat ggatagctac ttatcgacaa
     2221 acatttgata ttggctttgt ctcaatactc atggtctgcc tcgccttaag gctcaaatta
     2281 gggaagcaag aagccatttt ggctaaggtt ccctgggcta taattatcat gctctgcggc
     2341 atgagcttac taatgtctct agcggtcaag tcaggtttag ttacactgat agggcacctc
     2401 ataacaacaa ccattcctca tttctggtta cctctgttct tttgtgtgat agccggagtg
     2461 atgtcactct ttagttccac cctatccgtt gtagccccaa ctctctttcc catcattgct
     2521 accatcagtg ctcaaagtcc tcacattgat attcgcttac tcacaacagc cactattatt
     2581 ggcgctctat ctactaatat ctctcctttt tcatcagctg gttccctcat tcaactctcc
     2641 cttccccata tagaggaacg cagtcttgct ttcaaaaaac aaattcttct gggtgtgccc
     2701 atcagcttaa gtttggcact cttgaccatc tggattctca tgctgctagc ttccttaagt
     2761 tagatttatt gtctaactta aggttggact tggtataata aagtagacac aaaattaagt
     2821 ggaaacgagg aaaagtcatt atgccaagaa aaacctttga taaagccttc aaactctctg
     2881 ctgtaaaact catccttgag gaagagtagc ctgtaaaaat ggctagttca actttaaaaa
     2941 ttcatcccaa tagtctttat cgatggattc aagaatatga aaaatatgga gaaagtgcgt
     3001 tcccaggaca tgggagcgca cttcgtcatg ctcaatttga gacaaaaaaa cttgaaaaag
     3061 agcacaaact gctacaggag gaattagctc ttctaaaaaa gttccaggtc tttttgaagc
     3121 caaaccggaa ataaaatttc agtttctgaa ggaaaatagt gttagcttga atagtcatca
     3181 cgcttgtcaa actcttcaag tctcccgttc aggtttctat gcttatttaa aacgtcgtcc
     3241 ctcttcaagg catgttgaga atgaagcttt gaaggaaatg attaaagcca ttttctatga
     3301 acataaagag cgttacggaa gtgtacgaat tacccaagaa ctttgtagac gtggcatcca
     3361 tgtcaattat aaacgtgttg gtagactcct tcatcagcta ggtctctatg ctaaaggaag
     3421 tcgatatcaa tataaatact ataaccgtcg acgttcttca ttaactcgtc ccaatcttgt
     3481 taatcaatgt ttccaagcga ctggtaagaa taaattatgg ctaggagacc taacctacat
     3541 tcctacacaa gaaggaatac tttatgtgtc tgtctttata gacgtttata gtagaaagat
     3601 tgttggttgg gcaatgggcc gacgtatgca agacaagttg gtaacagaag ctttcaatca
     3661 agcttataat agagaaaagc ctaaagaagg ggtaatcgtc catacagatc agggttctca
     3721 atataccgga gcatgatttc aggacctact tagacagaaa aaatgtaagt ccaggatgag
     3781 tcgaaaaggt aatccatacg ataatgcact catggaatcc ttttataaaa cattaaaaag
     3841 agagcttgtc aatgatgctc atttcgcaac tattgagcaa gctcagcttg agatttttaa
     3901 atatagtgag acttactata accctaaacg tctgcattca gctttagggt atctctcacc
     3961 tgttgaattt gaaaaaatag ttactcatta gcttaacttt atgtctattt tttcttgaca
     4021 agtccagggt atgaagtccc tttatacaca tactttcaaa acagctctca atcctctgtc
     4081 cgctacaaaa atacgtcacc gctgccctcc agctgcctca ttcaacgaca aacttgaagc
     4141 cacaaacaga ctcatcaagg gcattaagta caatgccttt agatttcaga actttgacaa
     4201 ctttaaaacc aaaatcctca tcgctttgaa catccaaaaa gagagaacca atctgattct
     4261 ctctcgttgt taatgataag tcacccacta cagttgacaa tgagtctctt acttcctgtt
     4321 tactcattta atagctcttc tggttcccaa gattgattaa actcatggtc cttaaaaaga
     4381 gaagccaatc ctgttatttg tttcagtctt aaaatttcat ctttatccat tcctaaatgt
     4441 tttgcaatcc agttatcact tcgcccacac tcatgaagat cctttacaat ctgactcatc
     4501 aaatcaacat catgacttcc tctagctcta ttatgtcgaa tggtagaagc cattcgatag
     4561 tctaatgact tatcaataac agaaacaggt aatctcccac tttccctttc ataaatatct
     4621 gaataatcta gcattatacg atatctatga aaaccatcaa caatactata ccgatcctct
     4681 tccttatcgt aatagcaaac aatcggcata gtataaccat cagattttat actatcatac
     4741 aataatttca tttcaggcgg agcaactgaa ttcggattat aatcatttgg agaaatcttt
     4801 tcaattggaa ttgatttaat ctcatagact ggactaataa aattactcac tttctaactc
     4861 ctttaaactc gtcttatatt tctcttttaa aaaggctact attttttgtt gctctcttgt
     4921 caaaccaaac cccatagttc gacaaatatg atcgtttttt aaaatacaga aacacattcg
     4981 tttccagctt ggtatatcct ttgttgttac aatatcgtct gtatcatcag gaatttgatc
     5041 taaaaaaata acccttgaat gtttaaaaga ggtataattt gaaataccat ttctagcaat
     5101 acgatatcct ttttcaatta gttcattaat tgtttcctcc gcaagtcctc cgcctgtttt
     5161 atgccaaaag tctattgatt tattaaattt tctaacataa ttatttctta acctcactgg
     5221 tagcgttgat aaaagaaact gagtgtacga cttccacgaa tgaccctttg gtaaagcaat
     5281 tgatttatat cccattgctt tagtacgtcc ataaatattc gaaaagttaa ctccttgaac
     5341 acgtcccaaa agctttaccc aagtttcttg atctattatt ctataaagat ttaggctatc
     5401 aacagcatag tcttgaaaag gagatgcaac tctcatttgt gatggtttta aacccgcttt
     5461 atagaataga tcataaagtt cgttataaga ataagaaaac ttatagtaag catgccatat
     5521 gtcttccact gaccaatcat ataagggaga tgctgtccag acatctttga attgtttggt
     5581 aatccagcat tccttttgat aaccatactt tttattgata aaaccactat atctgtgtaa
     5641 ggattctgaa gcacggtttc ctaatataca aactgttttt tgatttccgt gaatttgttt
     5701 ataccaccta ccaaattgtt tcgctaaatc ctcctgattc attttataac gataagtagt
     5761 aattgaatta ttttccaaat taataacata atcttgagat ggcattggtc ttacccagat
     5821 atcttctgtt ttgtcatccc acggatacca aaacatttca taactactaa gtgcagttct
     5881 agtagccatc ggtaagcaca cccaatacaa agaaactttt tttctacctt ctaaagaagt
     5941 gaatgtttct tgaacatatt ttgttgttaa actgtactgt gcttcaaaat cttgatgaaa
     6001 cacacctatt tc
//
LOCUS       DAUGHY010000067         6011 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.067, whole genome shotgun sequence.
ACCESSION   DAUGHY010000067 DAUGHY010000000
VERSION     DAUGHY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.067"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..670
                     /locus_tag="VH752_001532"
     CDS             <1..670
                     /locus_tag="VH752_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ8289872.1"
                     /translation="TLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNSHISG
                     RSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNLEGML
                     CINLDISAYQGVARDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQDIIGQ
                     IIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRYLKKI
                     EADQ"
     gene            complement(792..1439)
                     /locus_tag="VH752_001533"
     CDS             complement(792..1439)
                     /locus_tag="VH752_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ8289873.1"
                     /translation="MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(1442..2101)
                     /locus_tag="VH752_001534"
     CDS             complement(1442..2101)
                     /locus_tag="VH752_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEQ8289874.1"
                     /translation="MKENKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKG
                     TKDLTLICNDAGFVDRGVGKMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQG
                     TFAEKIRIGAYGIGGFYTPTGVGTLVAEGKETKTIKGKTYLLEYPFEADVALIFANKA
                     DEMGNLQYSGSENNFNQLMAACAKTTIVQAREIVPVGTIQPECVHTPHIFVDYIVKEG
                     V"
     gene            complement(2113..3297)
                     /locus_tag="VH752_001535"
     CDS             complement(2113..3297)
                     /locus_tag="VH752_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ8289875.1"
                     /translation="MTKEVVITSAYRTPIGNFGGVFKSLSAVDLGVTVVTKILADTGL
                     KSDAIDEVIFGNVLHAGLGQNVARQVALNAGLSYDTPAFTIDMVCGSGLKAVELGAQK
                     IQTGNADIVLVGGTENMSQAPYVLQGQRWGSRMGDSKVVDTMLKDGLSDAFAGYHMGI
                     TAENIVQQYGLTREEQDAFAADSQRKAQLAIEKGRFKEEIAPVTIPQRKGEPLLVDQD
                     EYPKFGTTVDKLAKLRSAFIKDEGTVTAGNASGINDGAAAILLMSKEKAEELGLPILA
                     KITSYASAGVDPSIMGCGPIPATKKALAKAQLTIDDIDLIEANEAFAAQALAVSRDLG
                     FDNEKVNVNGGAIALGHPIGASGARILVTLLAEMAKRDVRHGLATLCIGGGQGQSIIV
                     TR"
     gene            3418..4329
                     /locus_tag="VH752_001536"
     CDS             3418..4329
                     /locus_tag="VH752_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ8289876.1"
                     /translation="MNISSLAYFIEIVNCDFNLTKAAQKSHISQPALSSYIKTIEKEE
                     GVVLFQRQKGRLVGLTSVGERFFENAKVVIASHEQLMADLRSESKKYKGEISIGIPQL
                     ISGIVFSELIPKLIEENPEVKFHIVETGAYELQKKLFLKEVDFAILLTPTTLDATIAD
                     IDILLRDQLCLYMSHKTFQETFDSNPNISIKALHRQNLVTFDKTFMINHQLTTLFEEA
                     NSLPVVKCYSASWDFLLNCTRYSSYLTILPRPITHFAHMDGLVEVQLTEHPKWEVVLA
                     SLKHNKTSHLKHYIKHTILDYFTNQRF"
     gene            complement(4397..5803)
                     /locus_tag="VH752_001537"
     CDS             complement(4397..5803)
                     /locus_tag="VH752_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HEQ8289877.1"
                     /translation="MNNIKTKKRFMDRYIDGFMKWMPESLFICFILTFLVVTMSVLMT
                     DSPFIGTEKTGGIIYGWVNGFWGLLSFAMQMTILLATGNAVASSPPAHKMFKSLAKLP
                     QTRTQIFIFSIVVGSIFGFLHWGLGMMVAIVFGKELLVQARQKGIKVHTPLFVATLFF
                     TFLPATSGLSGAAVLYSATPDYLRNSVADAYKQVVPESVPLTESVLNLPFISLLVVCM
                     LVPLCFALLAHPKDETKIMELDDEIYHHSLDTASHVVIARNTPAEKMNASRLVMYLVG
                     GAIVSYSLYHFSVVGLSGLDLNSFNFLFLGLGLLLCGQQGPEYYGSLFKDGVMSSWGL
                     VLQFPFYAGIFGIIQSTGLGLEISHFFVAISNGTTWPVFAYLHSALLNIAVPSGGSKF
                     VIEAPYIVPATIEVGNDLGKILQAYQLGDATTNLIVPFWALSYLSNFKLKFNQIVAYT
                     IPCVLVVTGIAIIYLFVF"
BASE COUNT         1914 a         1330 c          985 g         1782 t
ORIGIN      
        1 aacgctaaac tactggaaaa cggttatcac atttcttcac gatgtcttag gtgataacta
       61 tgaaattatt ttacatgtga ttgataaaaa tgatatctat attggagaac tcgttaatag
      121 tcatatcagt ggacggtcta aacagtctcc tctcaccacc tttgctcttg atttgattac
      181 caataaagtc tataaggaaa aagattttgt gaccaattac aaagcaatcg ttagtcctca
      241 gcacaaagaa gttcggggat ctactttttt tattaaagat aaaaagggta atctcgaggg
      301 aatgctatgt attaacttag atatttcagc ctatcaagga gtagctaggg acctcttaaa
      361 acttgtcaat ctcaatttag aacatttcat cccaactgct aaagaaccta aaacagtcac
      421 accccagcca gaagaagctg ttgaaatttt aacctctaac atccaagata ttatcggtca
      481 aattattgat ccctcgttac tgagacacaa tgtacatctt agccaagatg tcaaaataga
      541 cattgttgcc aagctttatg aaaaaggagt ctttcaacta aaaggagccg tctcaaaagt
      601 cgctgatatt ctatgtattt ccgaaccaag tgtttaccgc taccttaaaa aaattgaagc
      661 cgatcaataa ccaaatcacc ataaaacgcc agttaaaagt atttcagata agcatcacaa
      721 tctctgatat accgatgact gacgtttttt ttactcaata tgcctttatg gcaagaaaag
      781 gctactggtc attaaccttc agctatgctt agaggtacac ctgtcgcagc ttgaacctca
      841 tcaaaagtaa acccttcact gatttcaatt acttggatac catctggcgt tactttaaac
      901 acacccattt ctgtgataat aaggtcaacc acaccttttg ctgttaaagg taaggtacat
      961 tcttttagga gcttatgctt tcctttattg gtatgctcca tagctacgat gactttttta
     1021 gcaccaacta ataaatccat cgctcctccc ataccaggaa ccattttgcc tggaatcagg
     1081 taattggcga tatctccatt ttctgcaatt tctaaagcac ccaagacggt caaatcaaca
     1141 tgaccaccac gtataatgcc aaaagaatct gcactattaa agaaagcggc tcctggataa
     1201 accgtcacag gctgtcctcc tgcattaacg atagtcgaat tcggatcatc tgacttagga
     1261 cctagtccaa taaacccatt ttcagattga aaatacactg tcatttcttc aggcacaaaa
     1321 ttcgctactt tggttggtag accaatacct aaatttacca aagtaccatc ttctaattct
     1381 ttggccacac gctttgcaat acgattttga atctcttctt tacttaatgc aaccgccata
     1441 actatactcc ttctttaaca atgtaatcta caaaaatgtg tggtgtatga acgcattctg
     1501 gttgtatggt gccgactggt actatttctc gcgcctgaac aattgttgtt tttgcacaag
     1561 ctgccatcaa ttgattaaaa ttattttcag aaccactata ctgtaaattt cccatttcat
     1621 cagctttatt agcaaatatc aaggcgacat ctgcttcaaa tgggtattct aagagataag
     1681 tttttccctt gatggttttt gtttccttgc cttctgccac taaggttcct acccctgttg
     1741 gcgtgtaaaa gccaccaatg ccataagctc cgatgcgaat tttttcggcg aaggttcctt
     1801 gaggaatgag ctcaatcgtc gtttcaccgg cagtcatttg gcgcccagct tctttattta
     1861 atccaatatg tgttgcatag atggttttga attgatgatt agccaccatt tttccaacac
     1921 cacgatcaac aaaaccagca tcattacaga tcaaggttaa gtcttttgtg cctttgtcaa
     1981 ccagtgcatc aattaacgct tctggtgttc cattagccat aaaaccaccg accataatcg
     2041 tgtcaccatc tttaatatga ctgatagctt ctgctattgc tattctttta ttctctttca
     2101 tacggttacc ttctatcttg ttacgatgat actttgacct tggccaccac caatacaaag
     2161 cgttgctaat ccatgtctca catcacgctt agccatttct gctaataagg tgactaaaat
     2221 acgagcacct gatgctccaa tcgggtgacc caaagcaata gctcctccat tgacattaac
     2281 tttttcattg tcaaaaccaa ggtctcgtga aaccgctaaa gcctgagcag caaaagcttc
     2341 gtttgcttca atcaaatcaa tgtcatcaat tgtcagctga gcctttgcaa gagccttttt
     2401 cgtagcaggt attggtccgc agcccataat acttgggtct acacctgcac ttgcataact
     2461 agtgatttta gctaaaatag ggagccctaa ttcttcagct ttttctttac tcatcaataa
     2521 aattgccgct gctccatcat tgattcctga agcattacca gcagttactg tcccctcatc
     2581 tttgataaaa gcagagcgta actttgctaa cttatccact gtcgttccaa atttagggta
     2641 ttcatcttga tcaacgagta aaggttcacc tttacgctga ggaatagtga caggtgcaat
     2701 ctcttctttg aaacgacctt tttctatggc aagttgggct ttacgttggc tatcagctgc
     2761 aaaggcatct tgttcttctc tggtcagtcc atactgttgc acaatatttt cggctgttat
     2821 acccatgtga taacctgcaa aagcatcact caacccatct ttaagcatgg tatctactac
     2881 tttactatct cccatacgag atccccaacg ttggccttgt aaaacgtaag gtgcttgact
     2941 catgttttcg gtaccaccga ctaaaacaat atcagcgttg cccgtttgaa tcttttgagc
     3001 acctaactca actgctttta agccagaacc acagaccata tcaatcgtaa aagcaggggt
     3061 atcataagaa agccccgcat ttagcgctac ttgccgagca acattttgac ccagacctgc
     3121 atgtaacaca ttcccaaaga tcacttcatc aatggcatca cttttaagac cggtatcagc
     3181 taaaattttt gtaacaactg tcacccccaa atctacggct gataaagatt taaagactcc
     3241 tccaaaattt cctatcgggg tacgataagc agatgtgatc accacttctt tagtcataga
     3301 aacttctcct ttttcattgt tgaaacgctt acatttttat tatataatgt gaactataat
     3361 aaacaaatat cgttttttta tatatgctat aactaaacgt tatcgaaaga ggaaaatatg
     3421 aatatttcaa gcttggctta ttttattgag attgttaatt gtgactttaa cttaaccaaa
     3481 gcagcccaaa aaagtcatat ttcgcaaccc gcccttagta gttatatcaa gactatcgaa
     3541 aaagaagaag gagtcgtttt attccagcgt caaaaagggc gtctagttgg actaacctca
     3601 gtcggcgaac gcttttttga aaatgctaag gtggttatcg catcacatga gcaattaatg
     3661 gctgatttgc ggagtgaatc caaaaaatac aaaggagaaa tttcaatcgg tattcctcaa
     3721 ctcatctctg gcattgtttt ttctgagctc attccaaaac tcatcgaaga aaatcccgaa
     3781 gttaaatttc atattgtaga aacgggtgct tatgaactgc aaaaaaaact gtttctaaaa
     3841 gaagtcgatt ttgcgatttt attaacaccc actacactag atgctactat tgctgatatc
     3901 gacattctcc taagagatca actctgcctt tatatgagtc acaaaacctt tcaagagacg
     3961 tttgatagta atcctaacat ctctatcaaa gcattacatc gtcaaaactt agtaactttt
     4021 gataaaacct ttatgattaa tcatcagctt accaccttat ttgaagaagc aaatagtctt
     4081 cctgtagtca aatgttattc tgcctcgtgg gattttttat taaattgcac gcgctattcc
     4141 tcttacctaa caatcctacc aaggcctatt actcattttg ctcatatgga cggtttagtg
     4201 gaggtccaac tgaccgaaca tccaaaatgg gaagttgttc tagcctctct caagcacaat
     4261 aaaacaagcc atttaaaaca ttatatcaag cacaccatct tagattattt cacgaaccag
     4321 cgcttctaat tgcttgactc agaccttaaa aaaacacctg aaggaaacaa tcccaagcgt
     4381 ttcatatagt tatttcctaa aatacaaata ggtaaatgat ggcaatacct gtgaccacca
     4441 agacacaagg aatagtataa gccacaattt gattgaattt tagtttgaag tttgagaggt
     4501 aagataaggc ccaaaaagga actatcaagt ttgttgtggc atctcctaat tgataagcct
     4561 gcaaaatctt acctaggtca ttaccaactt cgatggttgc agggacgata tagggagctt
     4621 caatcacaaa ttttgagcct cctgatggta ccgcaatatt cagcaaagcc gagtgaagat
     4681 aagcaaagac cggccatgtt gtcccatttg aaatagcgac aaagaaatgg gaaatctcta
     4741 gtcctagacc tgtactttga atgattccaa aaataccagc gtaaaatgga aactgaagca
     4801 ccaagcccca agatgacatg accccatctt tgaataggga accataatat tctggtcctt
     4861 gttgaccaca aagaagcaac cctaagccta aaaataaaaa gttaaaacta ttgagatcta
     4921 gaccagacaa gcccaccacc gaaaaatgat aaaggctata gctaacaatg gccccaccaa
     4981 caagatacat cactaagcga gatgcgttca tcttctcagc aggtgtgttt cttgcaataa
     5041 caacatgtga agcggtatca agactatggt gatagatctc atcatcaagt tccatgattt
     5101 tagtttcatc ttttgggtgc gccaataaag caaagcaaag cggtaccagc atacacacca
     5161 ctaaaagact gataaatgga agattcaaaa ctgattctgt cagaggaaca ctttcaggaa
     5221 caacctgttt ataagcatct gcgacactat ttcgcaaata atctggagta gccgaataaa
     5281 gcaccgcagc accagataaa ccagaagtag ctggtaaaaa ggtaaaaaat aaagtagcaa
     5341 caaacaaagg tgtatgcacc ttaatccctt tttgcctagc ctgaacaagc aactctttcc
     5401 caaacacaat ggcaaccatc ataccaagac cccagtgtaa aaagccaaag atagaaccta
     5461 ctacaataga aaaaataaaa atctgagtcc tggtttgagg cagtttggca agggacttaa
     5521 acattttatg agcaggtgga gaactcgcta cagcattccc tgtcgcaagt aaaatagtca
     5581 tctgcatggc aaaggacaac agcccccaaa aaccatttac ccagccatag ataatcccgc
     5641 ccgtcttttc cgtaccaata aagggactat ccgtcatcaa cacagacata gtaacgacta
     5701 aaaaggttaa aataaaacag ataaaaagcg attctggcat ccatttcata aagccatcaa
     5761 tatagcgatc cataaacctt tttttggttt tgatgttatt catgttgtca attcctttca
     5821 cattgtccac aagcactttt gttcttttga tcagtaacct atcgataata ttaataagcg
     5881 tttctttagt tcatttagtt atcaataagt ttaaacccac aaaaaacact tttgttccct
     5941 ttttattatg ataacgtttt cataaacaaa caactatgat ttttttattg cttccataac
     6001 ccttatttat a
//
LOCUS       DAUGHY010000068         5975 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.068, whole genome shotgun sequence.
ACCESSION   DAUGHY010000068 DAUGHY010000000
VERSION     DAUGHY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.068"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            629..2386
                     /gene="pabB"
                     /locus_tag="VH752_001538"
     CDS             629..2386
                     /gene="pabB"
                     /locus_tag="VH752_001538"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEQ8289878.1"
                     /translation="MHRKTIIDFKELGQRYLFEEPLVELVAKSLDQVGPVIEKVQHYQ
                     QLGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKKDLPLDYDSITIP
                     NQWVSATQKEAYQKAIETIHREMQQGNTYQVNYTIQLTQELNAADSLAIYNKLVVEQA
                     AGYNAYIAHDEFAVISASPELFFKQEGNRLTTRPMKGTTKRGINSWLDQQEHDWLQAD
                     GKNRSENMMIVDLLRNDMGKICQIGSVCVDRLCEVERYSTVWQMTSTIVGDLKADCDL
                     IDILTALFPCGSITGAPKVSTMAIITSLEPKPRGIYCGSIGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVHQKTAFLYRHKQIFDLKTTAKVEHKKIALL
                     EQHLNRLKEAATYFAYPYNEKALQKQLSTYLENKDNAAYRLTISLSKDGKMRLSDQPL
                     EPLSADFLTAQLSLQDKDVTASPFTYFKTSYRPHIEQKSYEQVFYNQAGQLLETSIGN
                     LFIQLGQTLYTPPVAVGILPGLFRQELLATGQAQEKEVTLADLKEASAIFGGNAVRGL
                     YPLNLELTHLDALLAKSQA"
     gene            2419..2985
                     /locus_tag="VH752_001539"
     CDS             2419..2985
                     /locus_tag="VH752_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEQ8289879.1"
                     /translation="MILLIDNYDSFTYNLSQYLSEFDETIVLYNQDPNLYDMAKKANA
                     LIFSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGTLRLAKRVMHGRQ
                     STIETQGPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            3018..4286
                     /locus_tag="VH752_001540"
     CDS             3018..4286
                     /locus_tag="VH752_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEQ8289880.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTRYAFRTFNATIDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRTSGNLPIPRHLRDGHYAGSKELGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLSD"
     gene            4375..4571
                     /gene="ssrS"
                     /locus_tag="VH752_001541"
     ncRNA           4375..4571
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VH752_001541"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            4572..4644
                     /locus_tag="VH752_001542"
     tRNA            4572..4644
                     /locus_tag="VH752_001542"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4605..4607,aa:Lys,seq:ctt)
     gene            4785..5225
                     /locus_tag="VH752_001543"
     CDS             4785..5225
                     /locus_tag="VH752_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8289881.1"
                     /translation="MKIIEYKGNYRDDLIFMVLEAKNALRRIPSINPDLLTIETSYLE
                     KGDCFWLAVNEESRVIGSIAYSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYL
                     RKIGKSHVRIHLGQPKEQWFESYRFYPKHGYQFYDDTHLIKKLI"
     gene            5280..5750
                     /locus_tag="VH752_001544"
     CDS             5280..5750
                     /locus_tag="VH752_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEQ8289882.1"
                     /translation="MKIAIIGHSGSGKSTLARFLGQHYHCEVFHLDQLHFSSNWQERS
                     DHDMIADLSTCLLKQDWIIEGNYANCLYEERMSEADYIIYVNFSRFHCVYRAFKRYLN
                     YRGKTRPDMADNCQEKFDVAFVKWILLDGRSRNQLKKYQSVVQKYSHKTIVLTN"
BASE COUNT         1874 a         1271 c         1145 g         1685 t
ORIGIN      
        1 tcgtatttta agctatatta cgatgcataa tcctctcatt agtcaatgaa aacggataaa
       61 taaaacacaa ttaaatttaa cataattaac aaaggcggtg gttgttgatc gaaatgaaga
      121 tgccatagct gagatacata atgtaataaa tcaagtaaat aagactgcgc caaaaataaa
      181 agatagtgag cactttgata atttattaaa gaactctcat aataatctcg taataaggtt
      241 tccgttgaat taaacaacat aatatcaata accaaagatc cattctatta tgatgaaatt
      301 tattctggag ctgtaaaaga actattggct aaatgtttgt cttatgttga ttatttgaaa
      361 gatttagcta agcaatctaa gacagaacct aattttggtg aaaatcataa attatataaa
      421 actattatgg atgagttcaa gagtcaagtt aaaacactgg cattgaaaga taaaaaaatg
      481 gaatactgct cacctcacga taagagtata ttgtagtcaa tcaagactga acattcttag
      541 tgaacgttca gtctttttac gtggccaaaa gacagttgtt acctgttctc attaggctta
      601 gcaaccgctt ttatgatata atttgggtat gcatagaaaa acgattattg atttcaaaga
      661 gctcgggcaa cgctatcttt ttgaagagcc attagtagaa ttggtagcca agtccttaga
      721 tcaggtcggt ccagtcattg aaaaagtaca acactaccaa cagctgggct attacgtggt
      781 gggctatctc agttatgaag cagctgcttt ttttgacaat gccttacaaa cccataatga
      841 cagactaggc aacgagtacc ttgcctattt tacagtgcac aaaacctgtc aaaaaaaaga
      901 cctacctctt gattatgaca gcattactat ccctaaccaa tgggtcagcg caactcaaaa
      961 agaagcctat caaaaagcca ttgaaaccat tcaccgtgag atgcaacagg gcaatactta
     1021 tcaggttaac tataccattc aattaactca agaactcaat gcagctgaca gcttagccat
     1081 ttacaataaa ctcgtcgttg aacaggctgc tggctacaat gcctatatcg cccatgatga
     1141 gtttgccgtt atttcagcta gtcctgagtt gttctttaaa caagagggca acaggttaac
     1201 tactagacct atgaaaggca ccaccaaacg agggattaat agctggctgg atcaacaaga
     1261 acatgactgg ctccaagctg acgggaaaaa tcgctctgaa aacatgatga ttgtggattt
     1321 gctccgcaat gacatgggaa aaatttgcca aataggcagt gtttgtgttg ataggctctg
     1381 tgaggtcgag cgatactcta ccgtctggca aatgacctca actattgttg gtgacctcaa
     1441 agcggactgt gacctgatag acattctcac agctctcttt ccttgtggct ctatcacggg
     1501 ggctcctaaa gtctccacca tggcaattat cacatctctg gagccaaaac caagggggat
     1561 ttattgtgga agtattggca tttgcctacc tgatggtcgc cgtttcttca atgttcccat
     1621 tagaaccatc cagctcagtc ataatcaagc tacttatggt gtcggaggcg gcatcacttg
     1681 gcaaagcaag tgggaagacg aatacgaaga agtccatcaa aaaacagctt ttctctatcg
     1741 gcacaaacaa atctttgacc tcaaaacaac cgccaaggtg gaacacaaaa aaatagctct
     1801 ccttgagcaa cacctcaatc gccttaaaga agcagccact tattttgctt acccttataa
     1861 tgaaaaagct ttgcagaaac aactgtcaac ctatttggaa aataaggata atgctgccta
     1921 ccgcttgacg attagtttat ccaaagatgg aaagatgaga ctctctgatc agcctttgga
     1981 gcctttatca gccgattttc taacagctca gctctcttta caggacaagg acgtgacagc
     2041 ttcgcctttt acctacttta agaccagcta ccggcctcat attgagcaaa aatcttatga
     2101 acaggtgttc tataaccaag cggggcaatt attggaaacg tccatcggca atctttttat
     2161 ccagctgggt cagaccctct acacaccacc agtggctgtg gggattttac caggactttt
     2221 tcgccaagag ctgctagcta ctggtcaagc tcaggaaaaa gaggtgacac tagctgattt
     2281 aaaagaggct agcgctattt ttggaggaaa tgctgtccgt ggcctctatc ctctcaacct
     2341 tgagcttact caccttgatg ccctcttagc taagagtcaa gcctaaactg ccactcatct
     2401 tttgttatta aggaacctat gatactctta attgataatt acgattcatt tacctacaac
     2461 ctctcccaat atctaagtga atttgacgag acgattgtct tgtataacca agacccaaac
     2521 ttatatgaca tggccaaaaa agctaacgct ctaatctttt cacctggtcc tggttggccc
     2581 aaggaagcca accaaatgcc aaaactcatt caagactttt accaaacaaa acctatctta
     2641 ggagtgtgtc tgggacacca agctatcgct gaaactctag gaggaacctt acgcttggca
     2701 aaacgcgtca tgcatgggag acaaagcacc attgaaacgc aaggccctgc tagtcttttt
     2761 cgctccctgc cacaagagat caccgtcatg cgttaccatt ctatcgttgt ggatcagtta
     2821 ccaaaaggtt ttagcgtaac tgctagagac tgtgacgatc aagaaatcat ggcctttgaa
     2881 caccacaccc tgccactttt tgggctacaa tttcacccag aaagcatcgg aactcctgat
     2941 gggatgacca tgatcgccaa cttcatcgca gccattcccc gttaatctca tcccccaaaa
     3001 gaaaggagga cagttacatg cctgatcatt tagcgctcag aatgcgcccc aaaaccattt
     3061 cagaggtcat cggacaaaaa caccttgttg gcgaaggcaa aatcatccgt cgcatggtgg
     3121 aagcaaatag actatcgtcc atgatcctct atggccctcc aggaatcggt aaaacttcca
     3181 ttgcttctgc tattgctggt actactagat atgccttccg gacctttaat gccaccattg
     3241 acagcaaaaa acgtctccaa gaaattgcgg aagaagctaa attttctggt ggcttggtat
     3301 tattactaga cgagattcat cgccttgaca agacaaaaca agattttctt ttacccttac
     3361 ttgagaacgg caccattatc atgattggcg caaccactga gaatcccttc ttttcagtga
     3421 cacccgctat cagaagcagg gtccagattt ttgaattgga accactttct aacgaggata
     3481 ttaaaacagc cattcagcta gcaatttccg acaaagaacg gggatttcct tttttagtga
     3541 caattgatga tgaggcgcta gatttcattg ttaccgccac aaatggagac ctacgatctg
     3601 cttataattc cttggatcta gcagtcatgt ctacttctcc taatgaagat ggtagccgtc
     3661 atattagtct agagacaatg gaaaatagtc tgcagcgtag ttatattacc atggataaaa
     3721 acggggacgg gcattatgat gttttatctg ccttacaaaa atccattaga ggttctgatg
     3781 tcaatgctag cctgcactac gccgctcgtt tagtagaagc tggagacttg cctagtttag
     3841 caagacgatt gaccattatt gcttacgaag atattggctt agccaaccct gacgcccaag
     3901 ttcataccgt caccgcacta gatgccgctc aacgcattgg ttttccagaa gctcgtattc
     3961 ttatcgctaa cgtggtcatt gatttggctc tatcacctaa atcaaattca gcttatttag
     4021 ctatggatgc agctttagct gatttgagaa catcaggtaa cctccccatt ccaaggcatt
     4081 tgagagatgg gcattatgct ggcagtaaag aactgggcaa tgccaaagat tacctctacc
     4141 ctcacgccta tcctgaaaaa tgggtcaaac aacaatacct accagataaa ttagtaggac
     4201 atcattattt tgaagccaat gaaaccggta aatacgaacg cgcacttggt tcaaataaag
     4261 agcgtattga caagctctca gactgactgc tagttttaaa aatcgcaacc gatttcaaaa
     4321 atcgataatt ttgcttgaaa aaattttaaa aagtggtatt ataaaagtac agaaacattg
     4381 ctgtggcata cgaatccaca tctaagtgtt gaccgactat ttctttgtat tttcgggaaa
     4441 caaagactct ttctagtctg caagccgttt cacgcggacg caacttccaa gaaagagcgc
     4501 gttacccacc tgcttatact gcgcgggctc aatacaaaaa cgtggatcac cggtgccaat
     4561 acagcattct tgcctcctta gctcagttgg tagagcagta gactcttaat ctatgggtca
     4621 caggttcgag ccctgtaggg ggtatacaac tatgcatgaa aaaacctttg tttaaaggct
     4681 ttttttgctt gtcagaagag gatttgttcc atcatttatc cccgcagtaa tttttttatg
     4741 gtatgatacc aattaaagcg cttaagatta aggtggagac tgttatgaaa ataattgagt
     4801 acaaaggaaa ttacagagat gacctcattt ttatggtctt ggaagcaaaa aatgccttaa
     4861 ggagaattcc cagcattaac ccggatcttt tgacaattga aacaagttat ttggaaaaag
     4921 gggattgttt ttggctagcg gtcaatgaag aaagtcgtgt tattggctca atcgcttatt
     4981 cttctattcc caaaacaaat gaggtcttct tgcaccgtct cttcatcaaa ccaagcttaa
     5041 aacatcaagg cattggcagt caacttctga cgtttgcaga agattatcta aggaaaatcg
     5101 gcaagagtca tgttcgtatc cacttaggtc aacctaaaga acaatggttt gagtcttatc
     5161 ggttttatcc taaacatggt tatcaatttt atgatgacac gcatctcatc aagaaactaa
     5221 tctaaacaca gttcataccc tttatagtca tttcatacgt catttttagg aggtatccat
     5281 tgaaaattgc catcattgga catagtggct ctggaaagtc aaccttagca cgatttttag
     5341 gccaacatta ccattgtgag gtattccatt tagaccaact acacttctca tcgaactggc
     5401 aagaacggtc agatcatgac atgatagctg atttatcaac atgcctgctc aaacaagatt
     5461 ggattattga ggggaattat gccaattgtc tctatgaaga gcgcatgagt gaagctgatt
     5521 acattatcta tgttaatttc agtcggtttc attgtgtcta tcgagcattt aagcgttatt
     5581 taaactatag gggaaaaacg cgtcctgata tggctgacaa ctgtcaggaa aaatttgacg
     5641 ttgcgtttgt gaaatggatt ctactagatg gacgttcaag aaaccaatta aaaaaatacc
     5701 aatccgttgt ccaaaaatac tctcataaaa ccattgtctt aacaaattaa aagcagctaa
     5761 gtcattatat gaatacgatc aactaattag agtgcaaaac taaaataacc acgggtaatc
     5821 cgggctattc agactgaaag cctttgttta aaggcttttt tgcttgtttc ataactaatc
     5881 ggcttaatta aaagcgtcaa tccaccttta gtatcatgtc tcaattttca tttgatagtc
     5941 agcaagtata tctacaaaat tgttaatgat tattt
//
LOCUS       DAUGHY010000069         5591 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.069, whole genome shotgun sequence.
ACCESSION   DAUGHY010000069 DAUGHY010000000
VERSION     DAUGHY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.069"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            20..367
                     /gene="ssrA"
                     /locus_tag="VH752_001545"
     tmRNA           20..367
                     /gene="ssrA"
                     /locus_tag="VH752_001545"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            669..1289
                     /locus_tag="VH752_001546"
     CDS             669..1289
                     /locus_tag="VH752_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEQ8289883.1"
                     /translation="MTKKHLLTLLLISFFTSFLVACSTTKDKEPQPSDSEIITPRLHQ
                     AAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPSQHDPKTAGEVTIDAYTWQF
                     DQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDNYS
                     QASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLEE"
     gene            complement(1546..3810)
                     /gene="glgP"
                     /locus_tag="VH752_001547"
     CDS             complement(1546..3810)
                     /gene="glgP"
                     /locus_tag="VH752_001547"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEQ8289884.1"
                     /translation="MTRFTEYVETKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNSA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSIAEVEDVELEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNYWIEDDSWLVPTDI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEGVDYGLIKDGISFDKTQIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAVQLIIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHVKLAEIKFNNKLALKRYLKDNKDIELDEHSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKKGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNV
                     HLVENYNVTVAEHLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMENIYTFGKDSDTIINLYATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEE
                     RLDRLYKELISKDWFMTLIDLEEYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSR"
     gene            complement(3845..5338)
                     /gene="malQ"
                     /locus_tag="VH752_001548"
     CDS             complement(3845..5338)
                     /gene="malQ"
                     /locus_tag="VH752_001548"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEQ8289885.1"
                     /translation="MNKRASGILMHISSLPGKFGIGTFGKSAFEFVDLLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVDDELLEAADLCDITFGTNPEAVDYA
                     QLFQVRRPLLEKAVRAFVAEQENVCKLEAFETASSWLTDFAEFMALKEYFNNKALQDW
                     DDETVIKRQEDSLNNYRELLAKKITYHKVCQYFFYQQWSALKTYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEGQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
     gene            complement(5453..>5591)
                     /locus_tag="VH752_001549"
     CDS             complement(5453..>5591)
                     /locus_tag="VH752_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HEQ8289886.1"
                     /translation="NVENLGKMSFKQLLDIINSNEQNLSQTIFVPFSLKQRESVRDIS
                     P"
BASE COUNT         1725 a         1071 c          971 g         1824 t
ORIGIN      
        1 attggttaat tatctatttg gggttgttac ggattcgaca ggcattatga ggcatgtttt
       61 gcgtcccatc ggcagatgta aattgccagt taaatataac tgcaaaaaat acaaactctt
      121 acgctttagc tgcctaaaaa ccagctagtg tgacttctac aagattgctt gtgtcctgtt
      181 agaagtctca aaatagcaag ctacggttac gaaattgtct agtttcgtga caagagattg
      241 atagactcgc aaactaatgg cttgagttat gtgtctttag tttgttaaat gaagacataa
      301 cctatggacg tagacaaata tgttggcagg tgtttggacg tgggttcgac tcccaccagc
      361 tccatccatg cttaccgtaa gtaatcataa cttactaaaa ccttgttaca tcaaggtttt
      421 ttctttttgt cttgttcatg agtttccgtt atccctatag tcataccatc acacttaccg
      481 atactccatc aataccactc aaaaccttgt catatcagag ctttttgacc atttttttca
      541 tgaatatcta aaaaaaagaa tcaaaatagt actaaattcc ccatagcgca tgcgttatgg
      601 ggaatcatag ctatatcttg tttataattt atgatataat acagcaaaaa tagtttagga
      661 aataacgtat gacaaaaaaa cacttactaa cacttcttct catctctttt tttactagct
      721 ttttggtagc ttgttcaaca acgaaagata aagagcctca accgtctgat tcagaaatca
      781 ttactccccg actacaccaa gccgctcatc aagataaacg cgctaacttt gaaaaaatta
      841 aacttgcgac tgttgattcc tcatttacag gaggaacaag ccttgaagaa cttatttcac
      901 tctttggaga gcctagccaa catgatccaa aaacagcagg cgaagtaaca atcgacgctt
      961 atacttggca gtttgatcaa gttactctca ctgttaatct ttatcaaaat agtagtattg
     1021 ttaaaaccat ctctaatttt acctttgcaa gagagttagg cttatcgcaa aaggaatacc
     1081 aacaattaca aaaaggaatg tcttatgaag acgttaaaaa gatcttaaca gaacctgata
     1141 attatagcca agcgtcatct agtgatcatc aaactttgca agcgatttgg gttagtggct
     1201 taaagacaga tacaagcgga gctaatattt ctctcgtttt tgaaaataat cagttaacag
     1261 aaatgtctca ggtaggactt gaagaataaa aaatgagtaa tatattttgc taaaaagatt
     1321 gatttcggtg tacatcgaaa tcaatctttt tatatcattt taataaaaaa ctttagtgta
     1381 gtttcttata attacctcca tagatcgcta aacagacttg ctttacgctt aatgtaataa
     1441 ttaacctaag tctgaagtaa aatcataaga cagcagaaac tactgtctta tgatttagtt
     1501 taagcatgtt acggtaccgc taccccacat tgcaattatt attttttatc gtgaatgcca
     1561 aatatcttcg ttatactgct caatggtacg gtcagatgag aagaatccag cttttgcgat
     1621 gttatggacc acttttgtca tccataaatc ttggtcttca tagtctgcta acattttttc
     1681 tttcacttca atgtactctt caaggtcaat caaagtcatg aaccagtctt ttgaaatcaa
     1741 ttctttataa agacgatcaa gacgttcttc gttgccaaat gctagcaatt ctggactgat
     1801 aataaagttc actgctgctt taatagcagg atggttatca tagtaatctt ttgctacata
     1861 agaagcagtc gcataaaggt tgatgatggt gtcagaatct ttaccaaagg tgtagatatt
     1921 ctccatgcct gcaagctcag cgatttctac gttagcaccg tccattgtac caagtgttaa
     1981 agcaccgtta agcatgaatt tcatattacc agttccagaa gcttctttgg atgctagtga
     2041 aatttgctca gaaatatcag ttgctggaat caagtgctct gctactgtca cgttgtaatt
     2101 ttcaactaga tgcacattaa ggtatgggct tacttcaggg tcattgttaa tcaattcaga
     2161 caagcaaagg atcaagtgaa tgatgtcttg agcaatgatg taagcaggcg ctgctttacc
     2221 tccaaagata acagtgattt tacgttttgg aaggttgcct tttttaattt ccaaatattt
     2281 gtgaatcacg taaagagcat tcatctgttg acgtttgtac tcgtggaaac gtttgatttg
     2341 ggtatcaata atagaatgtt catcaagctc aatgtcttta ttgtctttaa ggtaacgttt
     2401 aagagctaat ttgttgttga atttgatttc agccaattta acatgaacag ctttgtcatc
     2461 tgcaaaggcc atcaattttt ctaattttgt tgcgtcagtc aagtactcat cgccaataag
     2521 ttctttgatg taatcagcca agtcttggtt agcaaattct agccaacgac ggaaagtaat
     2581 cccgttggtt ttgttgttga atttttctgg gtaaaggtca tagaaagctt ttaattcgct
     2641 gtttttcaag atttctgtgt ggagtgctgc taccccattg acacttgttg caaaatgaat
     2701 atccatatgg gccatgtgca cacgacctga ttcgtcaata atttgaacag ctgggtcaga
     2761 cacttctgaa cgaacaagag catccaattt ttcaatgatg gttaccaagt gtggcactac
     2821 ttcgtttaag taagcagttg gccatttttc aagggcttct gcaagaatag tgtggttagt
     2881 gtaaccaacc atatttttca caacagctac cgcttcatca aagtcaaagc catgtttttc
     2941 agtcaagaga cgaattaatt ctgggatgac cattgatgga tgcgtgtcat tgatttgcac
     3001 gtaagcgtag tctgcaaggt catgaaggtt tgaaccacgt tcgatagctt catcaatgat
     3061 taattgcact gcatttgaca ccataaagta ctgttggtaa atacggagca attccccatt
     3121 tttgtcagaa tcgtctgggt agaggaacaa ggtcaagttt ttagcaattt gcgttttatc
     3181 aaatgaaata ccgtctttga ttaatccgta atcaacgccc tcgatatcaa acaagttaag
     3241 gtagttttta gtgtcgcgtt tgtaacccaa aacatcaata cgatcaagac gagattttaa
     3301 ggtaaagttt ttgaagggca catcgtaaga aatgtctgtt ggaaccaacc atgagtcatc
     3361 ttcaatccag tagtttggct cagcttcttg ctcattgtgt ttgaagactt gcttaaacaa
     3421 cccacaatga tagtttaaac caaccccttc accattaata ccaagagatg aaatagagtc
     3481 aatgaaacat gatgccaaac gtcctaaacc accattccct agtgatggtt ctaattcaac
     3541 atcttcgact tctgcgatag atttgccagc agctgccaat tcttctttga tgtctttgta
     3601 aattcctagg ttaatcaggt tgtttgatag taatttacca atcaaaaact ctgctgagat
     3661 atagtaaact ttgcgtttag cagaattttt agccttgtgg ctagcttctt ctttgacaaa
     3721 gtttaataat gatagataaa tctcttcatt gctggcttgc gtaagtgatt ttcctagttt
     3781 agtttcaaca tattctgtaa agcgtgtcat gtcttcctct tttcaataat gtaacattta
     3841 aatattatct tgcgcgacca taaagatctg ttaaataaat aaggtagtct ttgtgatctt
     3901 ggttcaattc accgtcaagc atacgccatt gccaattacc tccaattgta tttggcatat
     3961 tcatacgact cttaccatct ttatctaata aatcttggat gcaaagaatg gctgtatcac
     4021 aaacagaagc aaagattgtt cttagcatgg cttttgtaat actttcatct gcatgtttgt
     4081 gaagatagtt atttacaaag tgtacttgtc cttcactaag attgtcatac catccgttaa
     4141 ccacctcgtt atcatgagtc cctgtgtaag cgacacaatt acggtcatag taatgtggca
     4201 aatctatact ttgactcgtt atatcaaata aaccaaattc taatatcttc atgccaggaa
     4261 agcctgttga tgctaatagc tgctctgctt tttcatcaat ataaccaagg ttttctgcga
     4321 tgataggaag ctctcctaag gcctcacgaa ccgcagaaaa taacgctata cctggggctg
     4381 aggcccaatg gccatttcta gcagttttat caccagcagg aatttcccaa aaatctgaga
     4441 agcctttaaa atgatcgatt cttagctgat catatagttt gaaactttct tgaatacgat
     4501 aaatccacca tgcaaaatta gacttttcat gagcagacca attgtaggtt ggatttcccc
     4561 acagctgacc atcttcacta aagccatctg ctggtacacc tgcaataaac aatggctttt
     4621 tatcactatc tactttaaat agctcaggca tcgtccaaac ttctacactg tccgcagaaa
     4681 cataaatagg catatcacca ataatttcta tccctttatg gttagcgtaa gttttcaagg
     4741 ctgaccattg ttggtaaaag aagtattgac agactttatg ataggtaatc ttttttgcaa
     4801 gcaactcacg gtaattatta agtgagtctt cttggcgttt aatgacagtt tcgtcgtccc
     4861 aatcttgtaa ggctttattg ttaaaatatt cttttaaagc cataaattca gcaaaatcag
     4921 ttaaccagct agaagctgtt tcaaaagctt ctaatttaca cacattttct tgttcagcaa
     4981 caaaagctct cactgctttt tctaaaagtg gacgcctaac ttgaaaaagc tgagcatagt
     5041 ctactgcttc aggatttgtg ccgaatgtaa tatcacacaa atctgctgct tctaaaagtt
     5101 catcatctac tagcaattca aaatcaatga aatgtgtgtt cccagcaata gctgaaaatg
     5161 actgataagg agaatctcca aaactcgtcg ttgttaaagg cagaatttgc caataggttt
     5221 gtttcgtctc tgctagaaga tcaacaaatt caaaagctga ttttccaaaa gtgcctatcc
     5281 caaatttccc aggcagggaa ctaatatgca ttaagatacc gcttgcacgt ttgttcatga
     5341 tttttctcct tgtgaaatct attcatgatg agattataac gcaagcgctt gcgtaagtca
     5401 accagtttcc taaaaaaagt aacattcctt aaaaatgcta ctttttttgt tcttaaggac
     5461 taatatcacg aacgctttct ctttgtttta aactaaaagg aacaaaaata gtctgactaa
     5521 gattctgttc attggaattg ataatatcaa gtaattgctt aaaagacatt tttccaagat
     5581 tttcaacatt t
//
LOCUS       DAUGHY010000070         5531 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.070, whole genome shotgun sequence.
ACCESSION   DAUGHY010000070 DAUGHY010000000
VERSION     DAUGHY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.070"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(1..1236)
                     /locus_tag="VH752_001550"
     CDS             complement(1..1236)
                     /locus_tag="VH752_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEQ8289887.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLLLSVAKLLAGYLLNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKSIFSGQQVDIDPLGAIVGIVSAFVMLGVYVFNKRLSKRVKSSALVAASKDNLAD
                     AVTSIGTSIAIIAASLHLPVIDHIAAMIITFFILKTAFDIFMESSFSLSDGFDSRHLK
                     KYEKAILEIPKIVSVKSQRARTYGSNVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQ
                     FSIYDIDIHVEPAMIPEEEIFDNVAKKLYRYEKLILSKVPDYDHYIAKSFQLIDANGQ
                     TVNYEQFLNQEIYYPSNFNHFQIESISQKTMLVTYQLNGNQRTSIWRCHESWSLLFHQ
                     ITPIAKKQLHHTHYRIVKM"
     gene            complement(1356..2678)
                     /locus_tag="VH752_001551"
     CDS             complement(1356..2678)
                     /locus_tag="VH752_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEQ8289888.1"
                     /translation="MIALVKLIDNLVWGPPLLILLVGTGIYLTSRLGLIQILKLPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFLGM
                     ATKYAEGVLAIKYRTKDANGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITHLTYFRIVFILM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     regulatory      complement(2806..2892)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3207..5525
                     /gene="pcrA"
                     /locus_tag="VH752_001552"
     CDS             3207..5525
                     /gene="pcrA"
                     /locus_tag="VH752_001552"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEQ8289889.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALALNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
BASE COUNT         1833 a         1075 c         1124 g         1499 t
ORIGIN      
        1 ttacattttt acaatacggt agtgtgtgtg atgtaattgt ttcttagcga taggggtaat
       61 ttggtggaag agtaaggacc aagattcatg acacctccaa atactggtac gttgattgcc
      121 atttaattgg taagttacca acatcgtttt ttgactaatg gattcaatct gaaaatggtt
      181 gaagttactt ggataataaa tttcttggtt caaaaattgt tcatagttaa ctgtttggcc
      241 attcgcatca atcagttgga aagacttagc aatgtagtgg tcatagtcag gaaccttact
      301 caaaattaat ttttcgtagc ggtagagctt tttggcgaca ttatcaaaaa tctcttcttc
      361 gggaatcatg gcaggctcaa cgtgaatgtc aatgtcataa atagaaaatt ggtcactcaa
      421 taactgctcc actttctctg taatagagtg actttcatag actgaaagat caggattcat
      481 ttcaagtaca atatcaagat agacattgct accataggtc ctagctcgtt gagacttaac
      541 ggagacaatt ttagggattt ctaaaatggc tttttcgtat tttttcaaat gacggctatc
      601 aaatccatca gataaactaa acgaactttc cataaagata tcaaaagctg ttttaagaat
      661 aaagaacgta atgatcatag cagctatatg atcgataact ggtaaatgca aagaagctgc
      721 tataatagca attgatgttc cgatagaagt aacagcatca gctagattat ccttagaagc
      781 agcgactaag gcactggatt ttacacgttt ggaaagacgt ttgttaaaga cataaacccc
      841 taacataaca aaagctgaaa cgataccgac aatagcccca agagggtcaa tatcaacttg
      901 ctggccacta aagatacttt taattgtgtg aatcagtact tggaaaccta caagaaacat
      961 aataaaagaa gtgacaaggc tggataagtc ttcaattttc caatgaccaa atttatgatt
     1021 ggcatcggct ggttggctag ctaagtgaag accaataagc agggctacat ttcccacaat
     1081 atccgataaa ttgttaaatc catcagcaat aagggaactt gcatttaaca aataaccagc
     1141 taataatttg gcaacactaa gcaacaagta gacaataata ctaacgattg ggccctttct
     1201 ggctagtttt aaatttgcaa tcggatcctg tgtcataata actcctttga tttcaacttt
     1261 atattatacc ataaaaaggg gattaagaaa aaagcctagc ttcttgaact tggcagaaga
     1321 agactaggta taactgggta gtactaacgt catgtttact taataaagta atgcttggtt
     1381 tctaaaataa caactggcga gagagctagg agggcaatca ggttaggaag tgccattaaa
     1441 ccattgacaa tatcagccaa aacccaaatc agttctaact taaggaagcc tcctaggcct
     1501 accattaaga taaagacaat acggaagtag gtgagatgtg taatgccaaa gaggaattca
     1561 aagcagcgtt ctccataata actccatcct aaaatcgttg taaaggcgaa gagaactagg
     1621 gaaaaggtta ggccaaaagt ccctaaattt ccaaacacag tggcaaaggc agattgggtc
     1681 agaggagccc cttcgagctg cccagtccat tggcctgtta ctaaaataga taggccggtt
     1741 agggtacaga taataatcgt atcaataaaa gttcctgtca tggagatcag tccctgctcc
     1801 acgggttcat tggttttagc tgctgctgca gcaataggtg cagatcctag tccagactca
     1861 ttggagaaga caccgcgtgc aattcctttt tgaatggcat ccttcatgag acttccagca
     1921 aagcctccaa tagcagctgt cggagtgaag gctgattgaa agactaaacg aataacagga
     1981 agtagctgtt ggtagtgact aaaaataact gctaaactag atagaatata aaaaatagcc
     2041 ataaaaggaa cgactttttc agccactttg gaaatggaat gaatgccacc aaaaataatg
     2101 gcagccacaa agatagctaa aacaatactg accatttgtg gggataagcc aaagctatga
     2161 cctaaagacg aggtgatcga gttcacttga gcaaaggttc ctattccaaa tagggcaact
     2221 aggatccctg aacccgcaaa aagaatggct aagggtttcc attttgtccc catgccatta
     2281 acaatatagt acataggacc accagaaata tgaccgttgg catcctttgt acggtattta
     2341 atcgctagta ccccttcagc gtacttagtg gccattccca aaaaagcggc aacccacatc
     2401 caaaagagcg ctccaggacc accagacttg atagcagtgg caaccccaac aatgttacca
     2461 gttccgacag tagcggcaag ggcagttgca agagcagcaa aggatgaaat atctccatgt
     2521 ccttcgtcat ctgaaaaaat gagtttaaag gctcttggta gttttaagat ttgaattaat
     2581 cctaaacgac tggtaaggta aatccccgtc ccaaccaata aaattaagag gggcggcccc
     2641 caaacaaggt tatcaattaa tttaacgagt gctatcatct ttctctccta aaagtcatcg
     2701 aagacgccaa aagaaaaaag ctatctcttt ctttgagaca acttctgaag caatgataaa
     2761 acagcaaaca caaaaaagac taagactgtt cttaatggtg tttgtatttt accttctgtc
     2821 cttttgcctg agagattgag cagtatgcct tgccccttcg gcgcctaata aggtctctcc
     2881 agaagtccgt ccatcaaaca gtcccgttca aaaactgaac acctgagagt ataactcctt
     2941 cggcgaaaaa aattcttctc ctgcatgaca tcattcggtt tattaaatta ggactatcat
     3001 accttaaaaa acgaatgaag tcaaccaaaa tagcaaaaaa ataaggaatg ctttaagaaa
     3061 tcgctattat ttttaaatat ctgatcctaa actagaaaaa aggaatctct ttacttttgg
     3121 ggtcttcttt gctatttttc ttggaaacat gatataattt taaggttgat aaaagagatg
     3181 tcactgaacg agattaagga gaaagcatga atcctttatt aaatggaatg aatgatcggc
     3241 aggcacaagc tgttcaaaca actgaaggcc ccttattgat tatggcgggg gctggttctg
     3301 gtaaaacaag ggtactgacc catcgtattg cctatttaat tgatgaaaaa tttgtcaatc
     3361 cttggaatat tttagctatt acctttacta ataaggctgc gcgtgaaatg aaagaaaggg
     3421 ccttggcctt aaatccagct actaaagata ccttaattgc tacctttcac tccatgtgtg
     3481 tacggattct gcgtcgtgag gctgatcaca ttggttacaa ccgaaacttc acaatcgtgg
     3541 atccaggtga gcaacgaacg ttgatgaagc gcattttaaa acaattaaat atagacccta
     3601 aaaagtggaa tgaacgttct attttaggaa ctatttcaaa cgctaaaaat gatttgcttg
     3661 atgaaaaggg atacgaggca caggctgctg atatgtatag tcaaatcgta gcgaggtgtt
     3721 acaaagctta tcaggaagaa ttacgacgta gcgaagcact tgactttgat gatttaatca
     3781 tgatgacgct acgcctgttt gacgctaatc ctgacgtgtt agcttattac cagcagcgtt
     3841 accaatacat tcatgtggat gagtaccaag ataccaacca tgctcaatat cagctaatta
     3901 aattactagc ttctcgcttt aaaaatattt gcgttgttgg agacgctgat cagtctatct
     3961 atggttggcg gggagctgac atgcaaaata ttcttgattt tgaaaaagat tacccagatg
     4021 ctaaggtagt gcttttagaa gaaaactatc gctctaccaa gaaaattctg caagcagcta
     4081 acgacgtgat taacaataac cgtaatcgcc gtcctaagaa actttggaca caaaatgcgg
     4141 atggggaaca actggtttat taccgggcaa atgatgagcg agatgaggca gtatttgtgg
     4201 cctcaaccat tagcaatatg agtcaagagc ttggaaaaaa cttcaaagat ttcgctgttt
     4261 tataccgtac caatgcccaa tctcggacca ttgaagaagc actcttaaaa tccaatattc
     4321 cttacaccat ggttggaggc accaagtttt acagccgtaa agaaattcgg gatttgattg
     4381 cctatttaac cattgttgct aaccctgctg ataatatttc ttttgagcgg attgtgaatg
     4441 agcctaaacg aggtgttggt ccaggaacgc ttgataaact gcgtcagttt gcctatgaga
     4501 gtgaccagtc tttattagaa gcagcttcaa atctattgat gtcccccttg aaaggtaaag
     4561 cagcacaagc tatcatggac ttggccaata ttctcggtca gctacgtcaa gacctagatc
     4621 agatgagtat caccgactta gcagaagctt tgttagaaaa aacaggctat ttagatagct
     4681 tacgcctgca aaatacattg gaaagccaag cccgtatcga aaatatcgaa gaattcttat
     4741 ctgttaccaa aaattttgac gagtcaagtg cctctcaaga agaagacgaa accggtgttg
     4801 atcgcttggg acgtttttta aacgatttag cactgattgc tgatacagat gattcccaag
     4861 cagaagctgc tgaagtaacc ttaatgaccc tacatgctgc caaaggcttg gagttccctg
     4921 ttgttttctt aattggtatg gaagaaggtg tcttcccctt atcacgtgcc tctgaagatc
     4981 cagatgaatt ggaagaagaa agacgtttag cttatgtcgg aatcacaaga gctgaagaag
     5041 ttctctttat gaccaatgcc aacaccagaa ccctctttgg taaaagtagt tataaccgtc
     5101 caacacgctt cttgaaagaa atttcagagg aactactctc ttataaagga ttggcaagac
     5161 cagcacaatc ttcatttggt gtgcgttttt ctactgaaac acacacacag tttggacaag
     5221 ggatgagcct atcagaagcc ttacaagctc gaaaggccca ggctcaagta agacaatcag
     5281 cacagccaat gcaggcacat acgataccat cagcttcaac tagctcagtt ttaccgtttg
     5341 gttctaatag ctctgtagaa gaggtcactt ggcagattgg cgatatcgct caccataaaa
     5401 aatggggaga cgggactgtg cttgaggttt caggctccgg aaaaacgatg gaattgaaaa
     5461 tcaaatttcc agaagttggt ttaaagaaat tactagcaag tgttgctcct attgagaaaa
     5521 aataagaaaa a
//
LOCUS       DAUGHY010000071         5456 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.071, whole genome shotgun sequence.
ACCESSION   DAUGHY010000071 DAUGHY010000000
VERSION     DAUGHY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.071"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(180..1322)
                     /locus_tag="VH752_001553"
     CDS             complement(180..1322)
                     /locus_tag="VH752_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEQ8289890.1"
                     /translation="MTINRVLKDDNLLQYTEKEFIEDMKILVAIDSFKGSVTSPELNT
                     SVAQALLSVDKQLVIETRAIADGGEGSLAALSQTVAGRWHQVKTIDLLRRPIKVAYYR
                     HAKQAFIESASIIGIDKITPNSVTYAQATSYGLGLAVKDAIQKGATQIEIMLGGTGTS
                     DGGKGFLESLNYDFMTGRSYLDTLASPVTLLGLTDVTNPYHGPQGFAAVFGPQKGGSL
                     SQIEETDQIASNFAKKVFCQTTIDLQTIPGSGAAGGLGGAIVLLGGTLTSGFSRIAEL
                     LNLDNSLQSCDLVITGEGCLDTQSQSGKVPVAIARMAKKYQVPTIALCGSVKIETGLA
                     AEDFLAVFSIQQQPISLEAAIDKTTTLSNIKILAANLMLLIAQFNK"
     gene            1603..4581
                     /locus_tag="VH752_001554"
     CDS             1603..4581
                     /locus_tag="VH752_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEQ8289891.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEEPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     TIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPRAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRK
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDNQTASAFQSYAQNDIF
                     DVNETEDTRQLIKNLESSDRRVVVTTIQKLNAMISQMESYDTPKFKKLKERLAHLNVV
                     FVVDECHRAVTPERQRYLTNTFRNSRWYGFTGTPIFVENKRAQLGDLAQTTEQQYGKC
                     LHQYTVKEAIHDKAVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLNSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLIQKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDKALSLYSNGGENDVLAPSWEEEKARFFEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAFQAFDKLFASVQVYSDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRREDDEAIPEINIDYELESVQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKEPKAYEGKSIVYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGMSTLKESLDYQAYKEKQSDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            4594..>5456
                     /locus_tag="VH752_001555"
     CDS             4594..>5456
                     /locus_tag="VH752_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ8289892.1"
                     /translation="MTKSKQPQYRFDGFKGEWEEKELGDIVQITMGQSPSSQNYTTNP
                     SDYILVQGNADIKNGYIFPRVWTTQITKQADKGDIILSVRAPVGDVGKTNYHVIIGRG
                     VAAIKGNEFIFQILKYLKEIGYWKRISTGSTFDSISSSDIKYAKIQIPSLPEQEAIGE
                     LFQTVDQLIQLQDQKLATLKEQKQTFLRKMFPPQIQKVPEIRLQGFKGEWEEKKLGEV
                     STHRSGTAIEKYFDSEGEFKVISIGSYGTNNLYVDQNIRAVSNELTNSKLVASGELTM
                     VLNDKTANGAI"
BASE COUNT         1815 a         1061 c         1006 g         1574 t
ORIGIN      
        1 ttacaggaac ctctctgaca gctttattag tatacgatat ctttactgtc attgtcaact
       61 cttttttaca aaatgtttct gtttttcata ctatgctaat gagttagctc gtgcgtctcc
      121 ttcttcatag gttactttta accgccagat gaatgctaca caatcgaaaa tagatgagtt
      181 tacttattaa actgagctat caatagcata aggttagctg ctaatatttt gatattagaa
      241 agtgttgttg ttttatctat cgctgcttct aaggaaattg gctgctgttg tattgaaaaa
      301 acagccaaaa agtcttctgc tgctagtcct gtctcaatct ttacagaacc acatagagca
      361 atcgttggaa cttgatattt tttagccatt cttgcaatcg caacaggtac cttgccagat
      421 tgtgattgtg tatccaggca tccttctcca gtaataacta aatcacaaga ctgaagggaa
      481 ttatccagat ttagtagctc tgcaatcctg ctaaaaccac tagttaaggt gcctcctaat
      541 aggacaatgg cacctcctaa tcctccagct gctccacttc ctggtattgt ctgtaaatca
      601 atggttgttt ggcaaaaaac ttttttagca aagttacttg caatttgatc agtttcttca
      661 atttgactaa gactcccacc tttttgaggg ccaaagaccg cagcaaatcc ttgagggcca
      721 tgatatggat tggtcacatc agtcagccct aatagcgtaa caggtgatgc taaagtatca
      781 agataagatc ttcctgtcat gaaatcatag tttaaacttt ccaaaaaccc tttgccacca
      841 tcagaagtgc ctgttcctcc tagcatgatt tcgatttgag tagctccttt ttgaatggca
      901 tctttaactg ccaaacccaa accataggaa gtggcttgtg cataggttac tgaattgggg
      961 gtgatcttat caataccaat aatactcgca gattcaataa aagcttgctt tgcgtgacga
     1021 taataagcga ctttaatcgg gcgtcttaat agatcaatgg tttttacttg atgccacctc
     1081 cccgccacgg tttgtgagag ggctgctaaa cttccttctc ctccatcagc gatagctctt
     1141 gtttcaataa ctaactgctt atcgacagaa agaagggctt gagctacact agtgttaagt
     1201 tctggagacg ttaccgatcc cttaaaagaa tctatggcta ccaaaatttt catatcttca
     1261 ataaattcct tttctgtata ttgtagcaga ttatcgtcct ttaaaaccct attaatagtc
     1321 aagctagatc tgtcttaagt tttttatcta ttttgagaac tcgacctttt tactaatgtt
     1381 gtcttattac tttcttcacc ttaaattatt gtcaagcaaa ctcaaaaata caagaacttt
     1441 ctcgattgca tttacatagt catagctttg tttagttgct aggatcttaa gcattcaact
     1501 aatcaaaaaa agactgtttg gaaggtttca atagctttgc cattctaaaa aggatccgcc
     1561 aatgatataa tattactata tcatgtcgaa tggggattaa aaatgggaaa tcaaactaaa
     1621 acggaacttg aacttgaaaa agagttgatc catctcttag aaacaggtga aagtcaatgg
     1681 acatatcgta aagaattaaa aactgaagat gctttatggg ataatttctt taagatctta
     1741 gcgcaaaata acacgcaata tctgaacgag gagcctttaa cagctagtga aaaagagcag
     1801 attaagaatc aacttaactt tgtcaattac tatgaagcgg ctaaatggtt agctggtgaa
     1861 aacggcattg ctaaagtaca agtgcaacgc gaagatgcaa aactaggaac gattcgttta
     1921 gaagtcgtta aagcagacaa tgtggctggt ggaacttctg tttatgagat tgccaatcaa
     1981 gttgctttct ctggttctcg tgatagacga ggtgatgtga cacttctcat caatggctta
     2041 cctatgatcc aaattgaatt aaaatctcag aatcatcaat gcattgaagc ttttaaccaa
     2101 gtcaaaaagt atgataaaga aggccagttt agaggtatct tttcaacctt acaaatgttt
     2161 gtggtctcaa ataaaacaga tacaaggtat atcgctgcag ctaaagaaaa taaattaaac
     2221 cctaacttct tgacccaatg ggtagatcaa aacaacaaac cacaaaaaga tctctttgcc
     2281 tttgccaaag aagtcctatc tatccctcgt gctcaccaaa tggtcatgac ttactctgtc
     2341 attgatgatg ataaaaaagc actaattctc cttcgtcctt atcagattca tgcaatcgaa
     2401 gccgtcgctg aagcgagtcg tcaccgcaag tctggctata tctggcacac aacaggttct
     2461 ggaaaaacct taacttccta taaagtggct cgcaatatcc ttcaaatacc ggccgttgaa
     2521 aaatccatct ttgtcattga ccgaaaggat ttagataatc agaccgcttc tgcttttcaa
     2581 tcctatgcgc aaaatgatat ctttgatgtc aatgagacag aagatacgag acaactcatc
     2641 aaaaatttag aatccagtga tcgtcgtgtt gtggtcacca ccattcaaaa actcaatgcc
     2701 atgatcagtc agatggaaag ttatgacact ccaaaattca agaaactaaa agagcgtctt
     2761 gcacacttaa atgttgtctt tgttgtggac gaatgccacc gtgctgtcac accggaacgt
     2821 caacgttatc tcacgaatac ttttcgtaac agtcgctggt atggctttac cggcactcct
     2881 atctttgtgg aaaataagcg tgctcaatta ggtgatttag cccaaacaac ggaacagcaa
     2941 tacggcaaat gtctccatca atacaccgtt aaagaagcca ttcatgataa agcggttcta
     3001 ggctttcaag ttgagtataa gacaaccatt cctgatatgc ctgaagacag tattccggaa
     3061 gaagcttatg atcatgaaga acatatgtta gctgtcttaa atagtatcat taatcaatca
     3121 cgtaagaagc ttggctttaa taatgggatt ggtcagacat tcgaaggctt attaaccgtt
     3181 aagagtattg ctcgtgccca agcctattat gatctgattc agaaagttaa agctggtgaa
     3241 acggatttag tcatttctaa gaaggtcaaa gaaaaactac cagactttcc aaaggtagct
     3301 attacttatt ctatcactga aaatgacaat gcgtctatca gccgacaaga taaaatgacc
     3361 aaaaatctag aagactataa tcacctcttt ggcactaact ttaccatcga taacttacaa
     3421 ggctataaca gagatttaaa tgatcgctta gcccgtaaaa aagataaatt caaagaccgt
     3481 catgaacaat tagacctcgt gattgtggtt gaccgtctct taacaggttt tgatgcgcct
     3541 tgtttatcaa cgatctttat tgatcgccag cctatgaaac ctcagcacat tattcaagct
     3601 ttctcacgaa ccaaccgtat ctttgaaagt agaaaacatt acggtcaggt tgtgactttc
     3661 caaacaccac ttcgttttaa agaagctgtt gacaaagcgc ttagcctcta ctctaatggg
     3721 ggtgaaaacg acgttttggc cccttcttgg gaagaagaaa aagcaagatt ctttgaaaaa
     3781 gtaacagtat taaaaaatat agttcctgat ccagacgctt tcccaactat tgagagcgca
     3841 cagacagctt ttctcaaaca atacgccaaa gcctttcaag cgtttgataa actctttgca
     3901 tctgtacagg tctatagtga ctttaatgaa acactgttga gtgaggttgg gttatcagat
     3961 gaagtgattg atacttataa aggcacttac caaaatgtca ttgcagaaat acgaaagcga
     4021 agagaagatg acgaagccat cccagagatc aatatcgact atgagctaga aagtgttcag
     4081 atggatgata tcaactatca ctatatcttg accttaattc aagcctttgt cgatcaagaa
     4141 caagaagctc ttcaagaaag gctcaatgac aatcctatgg atcaatacat ccaagatttg
     4201 gcaaaatcaa accctgctat ggctgactca ctagctgagt tatggcaaga tatccaaaaa
     4261 gaacccaaag cctatgaagg gaaaagcatt gtttatgagt tagataacct cattggtgat
     4321 aagatccaaa gggccatcaa acactttgcg gaccaatgga aggcagatcc tgacaaatta
     4381 gcctttgtgg cgactaatta tcatagtgct aactctacca aacaagtggg catgtcaacc
     4441 ttaaaagaat ccctagacta ccaagcctat aaagaaaaac aaagtgattc tgccatgaat
     4501 aaactcaaat acaagagcca atttgaacgg gagttagttc aattcattag agatcaaatc
     4561 caacccttaa aagggcacta ggaaaggaaa gagatgacaa aatctaaaca gcctcaatat
     4621 cgctttgacg gttttaaagg ggagtgggaa gaaaaagaac taggggatat tgttcaaatt
     4681 acaatgggcc aatctccatc aagtcaaaat tatactacaa acccttctga ttatattcta
     4741 gtacaaggaa atgccgatat aaaaaatgga tatatttttc cgcgtgtctg gacaacacaa
     4801 attacaaaac aagctgacaa aggtgatatt attcttagtg ttagggctcc agttggtgat
     4861 gttggaaaga caaattatca tgttattatt gggcgtggag ttgctgctat aaaaggtaat
     4921 gaatttatct ttcaaatatt aaaatacttg aaagaaattg gttattggaa aagaattagt
     4981 acaggttcaa cttttgatag cattagctct agtgatatca aatatgctaa aattcaaatt
     5041 ccctccctcc ctgaacaaga agccattggc gaacttttcc aaacggttga tcaattgatt
     5101 cagttgcagg atcaaaagct agcaaccctt aaagaacaaa agcaaacctt cctcagaaag
     5161 atgttcccac ctcaaataca aaaagtccct gaaatccgtt tacaaggttt taaaggagag
     5221 tgggaagaga agaaattggg ggaagtatct actcatagaa gtggaacggc aattgaaaaa
     5281 tattttgatt cggaaggtga atttaaagtc atttcaatag gtagttatgg aactaataac
     5341 ctctatgtag accaaaatat tagagcagtc tcaaatgagc ttactaattc taaattagtt
     5401 gcctccggtg aattaacaat ggtactcaat gataagacag cgaatggggc aataat
//
LOCUS       DAUGHY010000072         5379 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.072, whole genome shotgun sequence.
ACCESSION   DAUGHY010000072 DAUGHY010000000
VERSION     DAUGHY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.072"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..1183)
                     /locus_tag="VH752_001556"
     CDS             complement(<1..1183)
                     /locus_tag="VH752_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEQ8289893.1"
                     /translation="MKNNNKWIIAGLASFLFPLSIIFIILLSMGIYYNSDKTILASDA
                     FHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYALMCYYLGSFFSPLLFFFNLTSMP
                     DAIYLFTLIKFGLIGLAACYSFHRLYPKISAFLMISISVFYSLMSFLTSQMELNSWLD
                     VFILLPLVILGLNKLITENKTRTYYLSISLLFIQNYYFGYMIALFCILYALVCLLRLN
                     DFNKMFIAFVRFTAVSICAALTSALVILPTYLDLSTYGENLSPIKQLVTNNAWFLDIP
                     AKLSIGVYDTTKFNALPMIYVGLFPLMLSVIYFTLESIPLKIKLANACLLTFIIISFY
                     LQPLDLFWQGMHSPNMFLHRYAWSFSIVILLLACETLSRLKEVTQIKAGFAFIFLIIL
                     TS"
     gene            complement(1266..2885)
                     /locus_tag="VH752_001557"
     CDS             complement(1266..2885)
                     /locus_tag="VH752_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ8289894.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATEESILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
     gene            complement(2964..3836)
                     /locus_tag="VH752_001558"
     CDS             complement(2964..3836)
                     /locus_tag="VH752_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ8289895.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMASNIPLLLMCYFFLGKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
     misc_binding    3959..4200
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4255..5277
                     /gene="trpS"
                     /locus_tag="VH752_001559"
     CDS             4255..5277
                     /gene="trpS"
                     /locus_tag="VH752_001559"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEQ8289896.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPKQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIRIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEVKSA
                     MGINYF"
BASE COUNT         1867 a          906 c          981 g         1625 t
ORIGIN      
        1 gagatgtcag tataatgagg aaaataaaag caaaacctgc ttttatttga gtcacttctt
       61 ttagtcgaga gagagtctca catgcgagta ataggataac tatggaaaaa gaccaagcgt
      121 agcgatgcaa aaacatattt ggtgagtgca tcccctgcca aaaaagatca agtggctgta
      181 ggtaaaaact tattataata aaagttaaca agcaggcatt ggctaatttt atttttaaag
      241 ggatactttc taaagtaaaa taaataacac taagcattag gggaaataat cctacgtaaa
      301 tcataggcag agcattaaac ttggtagtat cgtacactcc tattgagagc ttagcaggta
      361 tatccaaaaa ccaagcattg ttcgtaacta actgttttat cggggataga ttctctccat
      421 aagttgacaa atctagatag gtaggaagta ttactagagc acttgttaaa gcagcacata
      481 ttgacacagc tgtaaaccta acaaaagcga taaacatttt gttaaaatca ttgagacgta
      541 aaagacaaac taaggcgtaa agaatacaaa aaagagcaat catgtagcca aagtagtaat
      601 tttgaatgaa taataatgat atcgaaagat aataagttct ggttttattt tctgtgataa
      661 gtttatttaa tccaagtata acaagtggaa gaagaatgaa aacatctaac caagaattta
      721 gttccatttg acttgtcaag aagctcatta agctataaaa aactgagatg gaaatcatca
      781 agaaagcact gatttttgga tataatctat gaaaagaata gcatgcagct aatcctatta
      841 acccaaattt tatcaaggta aacaaataga tagcatctgg catagaggtt aaattaaaaa
      901 agaaaagtaa tggagaaaag aaactgccaa gataataaca cattaaagca taaaaattta
      961 tccctagtcc gcttgtaaag gtataaaaaa aactatcaga accgtgcatg atgttacgaa
     1021 agttctgcgc aaaaataaca tactgatgaa aagcatcact agctagaatt gttttatcac
     1081 tattataata aatgcccatc gatagaagga tgataaatat aatactaaga gggaacaaaa
     1141 aactagcaag tccagcaatt atccatttat tattattttt cataaattat atggtctttc
     1201 gtttacaaat tttaaaaaag aggctaggaa gagatcttct tagccacctc atttatcata
     1261 aggttttatt tccaaagttc agcaactcgg gcttggactt ctggattgtc aaggaattcg
     1321 tcataagttt catctatacg gtcgataaca ccattttttg aaataacaac gatgtggtta
     1381 gcaatcgttt gaataaattc atggtcatga ctggcaaaga taacagattc tttgaagtct
     1441 ttgattccat cattaagact tgaaatagat tccaaatcta agtgattagt tgggtcatca
     1501 agaataagaa cgtttgattt caatagcatc aacttagaaa gcatgacacg cactttttcg
     1561 ccaccggaaa gaacgttaac agatttttta acttcgtctc cagagaaaag catccgtcct
     1621 aagaacccac gtaaaaaggt atcatcatct tcaccttttg aggcaaattg acgtaaccat
     1681 tcaagaatag attcttccgt ggcaaaatct tttgaattgt ctttaggcaa gtaagaacga
     1741 cttgttgtca ctccccattt aatggttcct tcgtagtcaa tatcatctgc taaagcacgc
     1801 ataagagcag ttgtctgaat atcattttga ccaataatag cagctttgtc acctggacgt
     1861 aaaatgaaac tgatattatc aatgattttc tctccatcga tcgttacaga aagattttca
     1921 acagtgagga agtcatttcc catttcacgc tctgctttaa aattaataaa tgggtattta
     1981 cggcttgagg gaacaatttc ttctaattca atcttatcaa gcattttttt acgagaggtt
     2041 gcttgttttg atttagatgc gtttgctgag aaacgggcaa cgaattcttg taattctttg
     2101 attttctctt ctgctttagc attgcggtca gcttgtaaac gagcggccag ctcagaagat
     2161 tgtttccaga aatcgtagtt acccacaaag agtttgattt taccaaagtc aaggtcggcc
     2221 atgtgagtac atactttatt caagaagtga cggtcatggg aaacaacgat gactgtattt
     2281 tcaaaatcaa tcaagaaatc ttccaaccat gaaatagatt gaatatcaag accattggtt
     2341 ggctcgtcaa gtaataaaac gtcaggctta ccaaagagcg ctttagcaag aaggactttc
     2401 actttatctc cattagccag ttcactcata ttttggtagt gaaggtcttc tggaatattt
     2461 agattttgga gaagttgaga agcttcactt tctgcctccc agccacctaa ttcagcaaag
     2521 ataccttcta gctcagcggc acgaacccca tcttcttctg aaaaatctgc tttcatatag
     2581 atagcgtctt tttctttcat gatattgtaa agttgctcat tacccatgat tacaacatca
     2641 atcgcacgtt cttcttcata atcaaaatga ttttggcgaa gaacagataa gcgttcgtca
     2701 ggtcctaaag agatatgacc agtacttggt tcaatatctc ctgcaaggat tttaagaaaa
     2761 gttgattttc cggcaccatt cgcaccgatt aagccgtaag tattgccggc tgtgaatttg
     2821 atattaacat catcaaatag ttttcgatca ctaaagcgta gtgatacatc agaaactgta
     2881 agcaatttgt cacctcattt tatttatcta agcttcattg tacctaaaat agtccccttt
     2941 ttcaatcaga aaagggctaa tcactacata ggcaataaaa cgtctttatc ttcacgtttg
     3001 tattgtttag tcaaactaaa tcctcttccc ataacttgag ctgctggcat gacaacaata
     3061 aaggctgtgt cgtcaatttc gaggattttt tcttggagag tgggtaattc gtgacttgat
     3121 acaatagcca ttaacataat tttatcagaa gtagtataac caccgcgaat aggaagttta
     3181 gtcactccac gatccataac agttgtgata tattctcgaa tagcttgata atctttggaa
     3241 ataatcataa catttttgga tgagtcaaag ccattttcca taacgctaat cacataacca
     3301 attacgaaaa gtccaatcgt agaatacatc acgtcatctg cagaaagtgc aataaatccc
     3361 ataaggacac taataccatc tacaattgtc attgcaactc caagagataa aggagagtat
     3421 ttatgaagta tttgtgttag aattccagtt cctcctgtcg aagagtttcc ccaaaaaacc
     3481 attcctaaac caatgccaca gataatacca ccaaaaatag ctgctaacaa ctgattgtgt
     3541 gtcagagtag gtaatgaatt ggttgaacgg atggcaatag gatatatcca agaaccataa
     3601 agtgttttaa taaaattttg ttttccaaga aagaagtaac acattaataa taagggaata
     3661 ttactagcca ttaaaaaaag cgacggacta atgccaaata gagcttttat aacaactgca
     3721 ataccaacca taccccctga tgcaatatga ttgtcgacaa acattgtatt gaaaccaata
     3781 gctgttatga atgagccaat tgtgacaagt agtaaatcaa tccctcgttt tttcatgctt
     3841 ttaatctcct ttataactgg ttaaaaatag tagactcatt atctcttaaa tgtactgaga
     3901 ccgtcaagtc atattttcaa aaaatctggt gaacttgaca agaagtttaa aatttcatag
     3961 aataaatttg aattagaaga gtattctttt catgttttta gagagttagt ggttggtgca
     4021 agctaacaac agaaaaggtg aaatggacta atgattattt gaaaggaaac cataactttc
     4081 aggttggcac cccttaccgt gcagcttctt tcattgagaa gattgagata gtatctcgtt
     4141 ttgataaagc agatgctaat tgaggtggta ccgcgtatta cttgtaataa cgccctcacg
     4201 ttttaatagc gtggggactt tttgctatat caagagatag ttagggagaa aatgatgaca
     4261 aaacctatta ttttaactgg agatagacca actggaaaat tacatttagg tcattatgtc
     4321 ggaagtctta aaaatcgtgt ctttttacaa aatgaaaaca agtataagat gtttgttttc
     4381 ttggccgatc aacaggcact gacggatcat gctaaagaat ccgaattaat tcaagaatct
     4441 attggaaatg ttgccttgga ttacctctct gttggtttgg atccgaagca atcaacgatt
     4501 tttattcaaa gtcagattcc agagctagct gaattgagca tgtattatat gaatctggta
     4561 tcattagcac gtttggaaag aaatcccact gttaaaaccg aaattgctca gaaaggtttt
     4621 ggcgaaagta ttccatctgg ttttttggtt tatccagtat cacaggccgc tgatattaca
     4681 gcatttaaag cgaatttagt acctgtaggt aacgaccaga aaccgatgat tgaacaaaca
     4741 cgtgaaattg tgagaagttt taatcatact taccatacag actgtttagt agaacctgaa
     4801 ggtatttatc cagaaaatga aaaagctgga cgcttacctg gtcttgatgg caatgccaaa
     4861 atgtctaaat cattgggaaa tggaatctat ctttcagatg atgcagatac cgttcgcaaa
     4921 aaagtgatga gcatgtatac tgatccaaat catattagaa tagaagatcc tggtcaaatt
     4981 gaagggaata tggtctttca ttatttggat atttttgcta gaaaagaaga tcaagctgat
     5041 atcgaagcaa tgaaagagca ttatcaaaga ggtggtttag gagatgtgaa aacgaaacgc
     5101 taccttctag atattttaga acgtgaatta gcacctattc gtgaaagacg tttggagtac
     5161 gctaaagata tgggagaggt gttccgtatg ttacaagaag gtagtcaaaa agcaagaact
     5221 gtggcagcca agactttatc agaagtgaag tcagcaatgg gtattaatta tttttaatgt
     5281 tttagagaaa aagctaagtt agtaatgata agataattac tattatcaaa gcaggaccta
     5341 atttgtcatt tctttacagg atagtagaga ttatgtttt
//
LOCUS       DAUGHY010000073         5058 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.073, whole genome shotgun sequence.
ACCESSION   DAUGHY010000073 DAUGHY010000000
VERSION     DAUGHY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.073"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            5..1553
                     /locus_tag="VH752_001560"
     rRNA            5..1553
                     /locus_tag="VH752_001560"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1605..1677
                     /locus_tag="VH752_001561"
     tRNA            1605..1677
                     /locus_tag="VH752_001561"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ala,seq:tgc)
     gene            1976..4878
                     /locus_tag="VH752_001562"
     rRNA            1976..4878
                     /locus_tag="VH752_001562"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4964..>5058
                     /gene="rrf"
                     /locus_tag="VH752_001563"
     rRNA            4964..>5058
                     /gene="rrf"
                     /locus_tag="VH752_001563"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1422 a         1046 c         1456 g         1134 t
ORIGIN      
        1 gcaaattaaa gagagtttga tcctggctca ggacgaacgc tggcggcgtg cctaatacat
       61 gcaagtagaa cgctgagaac tggtgcttgc accggttcaa ggagttgcga acgggtgagt
      121 aacgcgtagg taacctacct catagcgggg gataactatt ggaaacgata gctaataccg
      181 cataagagag actaacgcat gttagtaatt taaaaggggc aattgctcca ctatgagatg
      241 gacctgcgtt gtattagcta gttggtgagg taaaggctca ccaaggcgac gatacatagc
      301 cgacctgaga gggtgatcgg ccacactggg actgagacac ggcccagact cctacgggag
      361 gcagcagtag ggaatcttcg gcaatggggg caaccctgac cgagcaacgc cgcgtgagtg
      421 aagaaggttt tcggatcgta aagctctgtt gttagagaag aatgatggtg ggagtggaaa
      481 atccaccaag tgacggtaac taaccagaaa gggacggcta actacgtgcc agcagccgcg
      541 gtaatacgta ggtcccgagc gttgtccgga tttattgggc gtaaagcgag cgcaggcggt
      601 tttttaagtc tgaagttaaa ggcattggct caaccaatgt acgctttgga aactggagaa
      661 cttgagtgca gaaggggaga gtggaattcc atgtgtagcg gtgaaatgcg tagatatatg
      721 gaggaacacc ggtggcgaaa gcggctctct ggtctgtaac tgacgctgag gctcgaaagc
      781 gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat gagtgctagg
      841 tgttaggccc tttccggggc ttagtgccgg agctaacgca ttaagcactc cgcctgggga
      901 gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg cccgcacaag cggtggagca
      961 tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc cgatgcccgc
     1021 tctagagata gagttttact tcggtacatc ggtgacaggt ggtgcatggt tgtcgtcagc
     1081 tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccctatt gttagttgcc
     1141 atcattaagt tgggcactct agcgagactg ccggtaataa accggaggaa ggtggggatg
     1201 acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat ggttggtaca
     1261 acgagtcgca agccggtgac ggcaagctaa tctcttaaag ccaatctcag ttcggattgt
     1321 aggctgcaac tcgcctacat gaagtcggaa tcgctagtaa tcgcggatca gcacgccgcg
     1381 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc
     1441 cgaagtcggt gaggtaacct attaggagcc agccgcctaa ggtgggatag atgattgggg
     1501 tgaagtcgta acaaggtagc cgtatcggaa ggtgcggctg gatcacctcc tttctaagga
     1561 aatggaacac gtttatcgtc ttatttagtt ttgagaggtc ttgtggggcc ttagctcagc
     1621 tgggagagcg cctgctttgc acgcaggagg tcagcggttc gatcccgcta ggctccatca
     1681 ggatacaatc ctactaaact taatacaagt gaagttgaac acgcaactca cttcctagga
     1741 aaatagacaa tcttcccttg tgtgcaaggc ccacatggtc agattcctaa ttttctacag
     1801 aagtttcgct aaagcgagcg ttgcttggta tcctatataa tagtccattg aaaattgaat
     1861 atctatatca aattccacga tctagaaata gattgtagaa aagtaacaag aaaataaacc
     1921 gaaaacgctg tgactattta ataagttttc tagtttaaaa aaactaggtt aataaggtta
     1981 agttaataag ggcgcacggt ggatgccttg gcactagaag ccgaagaagg acgtgacaaa
     2041 cgacgaaatg ctttggggag ctgtaagtaa gcgctgatcc agagatgtcc gaatggggga
     2101 acccactaac taatggttag tatccataac tgttaaggtt atgagaagga agacgcagtg
     2161 aactgaaaca tctaagtagc tgcaggaaga gaaagcaaac gcgattgcct tagtagcggc
     2221 gagcgaaacg gcaggagggc aaaccgaagt gtttacactt cggggttgta ggactgcgac
     2281 gtgggacttt aaaaggatag aagaattacc tgggaaggta agccaaagag agtaaaagcc
     2341 tcgtatttaa aattcttttg agccctagca gtatcctgag tacggcgaga cacgtgaaat
     2401 ctcgttggaa tctgggagga ccatctccca accctaaata ctctctagtg accgatagtg
     2461 aaccagtacc gtgagggaaa ggtgaaaagc accccgggag gggagtgaaa tagaacctga
     2521 aaccgtgtgc ctacaacaag ttcgagcccg ttaatgggtg agagcgtgcc ttttgtagaa
     2581 tgaaccggcg agttacgata tgatgcgagg ttaagttgaa gagacggagc cgtagggaaa
     2641 ccgagtctta atagggcgat ttagtatcat gttgtagacc cgaaaccatg tgacctaccc
     2701 atgagcaggt tgaaggtgag gtaaaactca ctggaggacc gaaccagggc acgttgaaaa
     2761 gtgcttggat gacttgtggg tagcggagaa attccaaacg aacttggaga tagctggttc
     2821 tctccgaaat agctttaggg ctagcgtcga tgttaagtct cttggaggta gagcactgtt
     2881 tgggtgaggg gtccatctcg gattaccaat ctcagataaa ctccgaatgc caacgagata
     2941 tcatcggcag tcagactgcg agtgctaaga tccgtagtcg aaagggaaac agcccagacc
     3001 accagctaag gtcccaaaat aactgttaag tggaaaagga tgtggggttg cacagacaac
     3061 taggatgtta gcttagaagc agctattcat tcaaagagtg cgtaatagct cactagtcga
     3121 gtgaccctgc gccgaaaatg taccggggct aaaacagttt accgaagctg tggatgacac
     3181 aaaagtgtca tggtaggaga gcgttctatg tgtgaagaag gtgtaccgtg aggagcgctg
     3241 gaacgcatag aagtgagaat gccggtatga gtagcgaaag acaggtgaga atcctgtcca
     3301 ccgtaagact aaggtttcca ggggaaggct cgtccgccct gggttagtcg ggacctaagg
     3361 agagaccgaa aggtgtatcc gatggccaac aggttgatat tcctgtacta gagtatatag
     3421 tgatggaggg acgcagtagg ctaactaaac cggacgattg gaagagtccg gctaagcagt
     3481 gaggtgtaag atgagtcaaa tgcttatctt tataacattg agctgtgatg gggagcgaat
     3541 tttagtagcg aagttagtga tgtcacactg ccaagaaaag cttctagcgt ttaatgatac
     3601 tctacccgta ccgcaaaccg acacaggtag tcgaggcgag tagcctcagg tgatcgagag
     3661 aactctcgtt aaggaactcg gcaaaatgac cccgtaactt cgggagaagg ggtgctgact
     3721 taggtcagcc gcagtgaata ggcccaagca actgtttatc aaaaacacag ctctctgcta
     3781 aatcgtaaga tgatgtatag ggggtgacgc ctgcccggtg ctggaaggtt aagaggaggg
     3841 tttagcgcaa gcgaagatct gaattgaagc cccagtaaac ggcggccgta actataacgg
     3901 tcctaaggta gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaatgat
     3961 ttgggcactg tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta
     4021 cccgcgacag gacggaaaga ccccatggag ctttactgca gtttgatatt gagtatctgt
     4081 accacatgta caggataggt aggagccatt gacttcggga cgccagtttc gaatgaggcg
     4141 ctgttgggat actacccttg tgttatggct actctaaccc agataggtta tccctatcgg
     4201 agacagtgtc tgacgggcag tttgactggg gcggtcgcct cctaaagagt aacggaggcg
     4261 cccaaaggtt ccctcagatt ggttggaaat caatcgcaga gtgtaaaggt ataagggagc
     4321 ttgactgcga gagctacaac tcgagcaggg acgaaagtcg ggcttagtga tccggtggta
     4381 ccgaatggaa gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct
     4441 cccccaagag ttcacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct
     4501 ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca cgcgagctgg
     4561 gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgtagga aatttgagag
     4621 gatctgctcc tagtacgaga ggaccagagt ggacttaccg ctggtgtacc agttgtcttg
     4681 ccaaaggcat cgctgggtag ctatgtaggg aagggataag cgctgaaagc atctaagtgc
     4741 gaagcccccc tcaagatgag atttcccatg attttatatc agtaagagcc ctgagagatg
     4801 atcaggtaga taggttagga gtgtaagtgt agcgatacat gtagcggact aatactaata
     4861 gctcgaggac ttatccaaaa agaaatattg acaacgttac ggattcttgt tagactatag
     4921 atattcaatt ttgagtgggt tatgaaaata gcacatttac aagttaagtg acgatagcct
     4981 aggagataca cctgtaccca tgccgaacac agcagttaag ccctagaacg cctgatgtag
     5041 ttgggggttg ccccctgt
//
LOCUS       DAUGHY010000074         4716 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.074, whole genome shotgun sequence.
ACCESSION   DAUGHY010000074 DAUGHY010000000
VERSION     DAUGHY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.074"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(71..610)
                     /locus_tag="VH752_001564"
     CDS             complement(71..610)
                     /locus_tag="VH752_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEQ8289897.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALLISGVVLVDVLGAIWLAAYTGMSLVTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTKQ"
     gene            complement(614..925)
                     /locus_tag="VH752_001565"
     CDS             complement(614..925)
                     /locus_tag="VH752_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289898.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCAFCQTYYACYHCHDQ
                     LTDHAFVPTGHQETSPVICGHCRKLLSLAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(1143..2285)
                     /gene="tgt"
                     /locus_tag="VH752_001566"
     CDS             complement(1143..2285)
                     /gene="tgt"
                     /locus_tag="VH752_001566"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEQ8289899.1"
                     /translation="MTDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVIKNAKFAEDFTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
     gene            2504..3367
                     /locus_tag="VH752_001567"
     CDS             2504..3367
                     /locus_tag="VH752_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEQ8289900.1"
                     /translation="MSIKAIKGQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAASYFPLLLGLILSLFILSASFTMIDVVRHFRQKVSFAESTTAFSKEFF
                     GNLLVLAITKWLFFLIWSLIWFFGLFIFLSGLSAFLVNAKSGGSTVISLIFLLFGAVL
                     SLIGFGIYINRYYAYSLSEYLLYDKVKEGTYLGAIAVIETSVAMMKGYKWKLFFLQLS
                     FTGWFLLNIVTFGLLNIYLLPYFTTANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKK
                     PTPMMYDDQKD"
     gene            3564..>4716
                     /locus_tag="VH752_001568"
     CDS             join(3564..3894,3894..>4716)
                     /locus_tag="VH752_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ8289901.1"
                     /translation="MIRLIDRYGVTIVQKCKNHYYSPELKQKIINKVLIDGQSQKQTS
                     LDYALPTSSMLSRWIAQYKKNGYTILEKPRGRPSKMGRKRKKNLEEMTEVERLQKELE
                     YLRAENAVPKKAERIPLEGRSKAQRATEIIQALRNPFPLEMLLEILDLSRSTYYYQVK
                     RLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELRNRGFVVNHKKVQRLMKVMGLAA
                     RIRRKRKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTELALPEGKLYLLPVLDG
                     YNSEIIDFTLSRSPNLKQVQTMLEKTFPADSYSGTILHSDQGWQYQHQSYHDFLESKG
                     ILPSTSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITDYI"
BASE COUNT         1501 a          930 c          920 g         1365 t
ORIGIN      
        1 ccaaaaacag gtcctagcag ataaatctgc caagacctgt tcaacacaac ttaactattt
       61 gatgattaga ttactgtttt gtattcaaaa aactatcttt gtatttaaca gcgataatgg
      121 tcgcaatgat tgctttgatg gtatctcctg gaataaagac caagtttgac aaaagcgatg
      181 tcacaagaga catgccagtg taggctgcca accagatagc acccagaaca tcaaccagta
      241 caactcctga gattaaaaga gctaggaatg ccaatggcgt gctttttgag aaccagttgc
      301 ggtaaagtaa tgaaaagaca ataggcacaa gaaggtaggc aataacatag ccagcagatg
      361 gccccatcaa tactggaatg gttgttcgtg agcctgagaa aacaggtaag aaaaggccaa
      421 tcactaaaaa taagaaaaca gataaggttc cttttttacc acccagcatc aatccagcca
      481 acatgacacc taagttttgt aaaacaatag ggactgggat aaagcctagc gggatagcag
      541 gaataaagcc taaaataata atcaaagtgg tcatcatagc caccttaaca agttctttgg
      601 ttgtaaacat ctgttacgac tcctttaaaa aatagatatc cttgtgccta tgacaggctg
      661 gattaaaggg actttgacaa taaggacagc atccacaacc atactcagct aggctcaaga
      721 gctttcgaca atgcccacaa ataacaggag atgtttcttg atgtcctgtt ggcacaaagg
      781 catgatctgt taactggtca tggcaatggt aacaagcata ataagtctga caaaaagcac
      841 atttgagccc aacaatatca agcggggtat gataatgtag gcaccgatac tcttggtcaa
      901 ggtcaattcc aaaacagtcc gtcatatctc atcctcttgt aacagtttaa tcaaaagctt
      961 atgatttgtc aactatattt ttacaaaagg gtaactgttt tctgaaaaag aacacgtaaa
     1021 acaagaactt ccctttaaag agtgacctat cagttgaaag caataggaca tccgtctaca
     1081 caaaaaaacc tgaaaccaaa gacgatagat cgccctcagt tccaggatca tagcattttt
     1141 aattagaaat tacggtttga tttattataa ccataccgtt caagaaaatc ttggcggaat
     1201 tcaagtaagt tatcgtccat aatagcctga cgtacttttt tcatcaagtt aaccaagaaa
     1261 taaaggttgt ggtagcttgt caaacgaata ccaaaagttt cgtccgcttt tagcaagtga
     1321 cgaatgtagg cacggctata gttttgacaa gtgtagcagt cacagtcatg atcaagtggg
     1381 gtaaaatctt cagcgaattt ggcattttta atcacaagac gcccctcact agtcatgcaa
     1441 gttccgttac gggcgatacg cgttggcaag acacagtcaa acatatctac accacgaatc
     1501 acaccatcaa tcaagctatc tggtgctccc actcccatca ggtaacgagg tttattttct
     1561 ggtaagaggg gagtcgtaaa gtcaaggacg gcattcattt cttcatggct ctccccaaca
     1621 gctaaaccac caatagaata acctgggaaa tccatagcca ccaaatctgc cgcagactga
     1681 cgacgaaggt cctcaaatcc agctccttgt acaattccaa ataatccttg gtcatgagga
     1741 cgacgatggg cttttaagcc acgttctgcc caacggctag tacgttcaat ggattttttc
     1801 acgtaatcat aaggttggta aaattgcggg cactcgtcaa aactcatcat gatgtctgag
     1861 ccaaggttat tctgaatgga aatggctttt tcgggtgata gaaacatttt tgagccattc
     1921 aagtgatttt tgaaagtcac tccttcttca gtgatgtttc gtgaatcggc taaagagtaa
     1981 acttgaaaac cacccgaatc ggttaagatt ggctgatccc agttcataaa cttgtgcaga
     2041 ccaccagagc gagcaatcaa ctcatctcct ggacgcaacc agaggtgata agtgtttgat
     2101 aaaataatcc ctgatccaat tgcttttaac tcttctggag actgtgtttt aacagtagcc
     2161 tgagttccca ctggcataaa cattggtgtc ggaaaggtcc cgtgaggggt gataatttcg
     2221 cccaagcgtg caccggtgtg cttttctgtt ttgataaggc gatacttaat cggataatct
     2281 gtcatgtgct ttcttccttg ctatcaagat aaacagtctt gagctttttt gatctgcctg
     2341 agtttccatc ataatgaagg gcttcaagat gacatgttgt tctagcgcct atcattttat
     2401 caaaaaatcc ataatttgtc atgctttccc aaaaaagtat agtcaaaata atggttagct
     2461 gtctcatttt gttttatggt ataatgaaat aaaggagtct gttatgtcaa ttaaagcaat
     2521 caaaggccag gctagagaca cgttaaaaaa tctctctggc aagtatttac tatttttaat
     2581 tccaaccctt ctctttatgt ttcatttcgg tattgagatt catcaaggtt acgtcctatc
     2641 tagtggtatt gaagtctcgc tagcggctag ttatttccca ttactactag gacttatcct
     2701 tagccttttc attttatcag catcctttac gatgattgat gttgttaggc attttcgtca
     2761 gaaagtttct tttgccgaat caacaacagc cttctcaaaa gaattctttg ggaacctgct
     2821 ggttttagcg atcactaagt ggctattctt tctcatctgg tcactaattt ggttttttgg
     2881 cctctttatt ttcctttccg gactttcagc gtttttggtc aatgctaaat caggtggctc
     2941 aacagtcatt tcactgatct ttttactatt tggagcagtt ttgtccttaa ttggttttgg
     3001 tatttatatc aaccgttatt acgcttatag tctatcagaa taccttctct acgataaggt
     3061 taaagagggg acttatctag gagctatagc cgtcattgaa acaagtgtag ctatgatgaa
     3121 aggttataaa tggaagcttt ttttcttaca acttagcttc accggttggt ttctactgaa
     3181 tattgttact tttgggttac ttaatatcta tcttttacca tattttacaa cagccaatgt
     3241 cattttttat gatcaactca agaaacgttt caaagacaaa gatgacccaa ttgaaggaga
     3301 acatttaagc cttcatcagc ttaataaaaa gcctactcct atgatgtatg acgatcaaaa
     3361 agactaaata aatcctccat ccataaaaat agcccttgaa ctgcacccca aaagttagac
     3421 acaaaatcta acgattgggg tgctattttt ttatgaaatt taatcaagaa acgaaagtta
     3481 agatttatga gttacggcaa atgggagagt ccattaaatc catatcaaaa aagtttgata
     3541 tggcagaatc cgatctcaaa tatatgattc gcttgattga taggtatggg gtaaccattg
     3601 ttcaaaaatg taagaatcat tattattctc cagaactgaa gcaaaagata ataaataaag
     3661 tcctgattga cggccaatct cagaaacaaa catccttaga ttatgcttta ccaacttcta
     3721 gtatgctctc aaggtggata gcgcaataca agaaaaacgg ttatactatt cttgaaaaac
     3781 caagagggag accgagtaag atggggcgta aacgtaagaa aaatttggaa gagatgacag
     3841 aggttgaacg tcttcagaag gaattagaat accttagagc ggagaatgct gtgctaaaaa
     3901 agctgagaga ataccgcttg agggacgaag taaagctcaa agagcaacag aaatcattca
     3961 agcattaagg aatccatttc ccttagagat gctacttgaa atccttgatt tatcacgctc
     4021 cacctattac tatcaagtga agcgactagc tcaaggagat aaggatatag aactaaagca
     4081 tgtgattcga gagatttatg atgagcataa aggtaattac ggttatcgtc gtattcatat
     4141 ggagttgcgt aaccgaggat ttgtggtcaa tcataagaaa gttcaacgtc tcatgaaagt
     4201 catgggccta gcagcccgca ttcgtcggaa gcgcaagtat tcttcttaca aaggagaggt
     4261 tggtaagaag gctgataatc tgattaaacg tcacttcgaa ggctctaaac cttacgaaaa
     4321 atgttacact gatgtgacgg agttggcctt acctgaagga aaactctact tattacctgt
     4381 tcttgacggc tataacagtg agattattga tttcactctg tctcggtctc ctaacttgaa
     4441 acaggttcaa accatgcttg agaagacttt tccagcggat tcatacagcg gaactattct
     4501 tcatagtgac caggggtggc aataccaaca ccagtcctac catgattttt tagagtctaa
     4561 aggtattttg ccatccacgt cccgcaaagg gaatagcccg gataacggca tgatggaatc
     4621 cttctttggc attctcaaat ctgaaatgtt ttatggactc gagacaactt atcaatcact
     4681 tgacaagctt gaagaagcta ttacagatta catttt
//
LOCUS       DAUGHY010000075         4673 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.075, whole genome shotgun sequence.
ACCESSION   DAUGHY010000075 DAUGHY010000000
VERSION     DAUGHY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.075"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            141..3167
                     /locus_tag="VH752_001569"
     CDS             141..3167
                     /locus_tag="VH752_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEQ8289902.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKAVTEN
                     TESEKQITSGSQLEQSKESLSLNKTVPSTSNWEICDFITKGNTLVGLSKSGVEKLSQT
                     DHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKEIINEG
                     EVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDL
                     PDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASF
                     QDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPYGLATENTYVNPD
                     KSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQHNGVTITEI
                     GDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASDDLEEIKEGA
                     FMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANNLIFMGS
                     KVKTLGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKK
                     NHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLS
                     STMVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQ
                     FFLGRVDLDKAIAKAEKALVTKKATKNGQLLERSINKAVLAYNNSAIKKANVKRLEKE
                     LDLLTGLVEGKGPLAQATMVQGVYLLNTPLPLPEYYIGLNVYFDKSGKLIYALDMSDT
                     IGKGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLSQLTSIRQVPT
                     AAYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRALQSNPKTNRGRHS
                     AILPRTGSKGSFVYGILGYTSVALLSLITAIKKKKY"
     gene            3187..3588
                     /locus_tag="VH752_001570"
     CDS             3187..3588
                     /locus_tag="VH752_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEQ8289903.1"
                     /translation="MITQEMKDLINNQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAQGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEINSESN"
     gene            complement(3782..4657)
                     /locus_tag="VH752_001571"
     CDS             complement(3782..4657)
                     /locus_tag="VH752_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEQ8289904.1"
                     /translation="MPVSKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESSNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYCALHHELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLKESTTEKHCKKSIRLICQRYNINSVTVEIDTSLN
                     EHQHHCSSLSGIEVN"
BASE COUNT         1650 a          741 c          887 g         1395 t
ORIGIN      
        1 agtactagag tatttaaaat aagtgataga cttggatatt attaggtcga ctagtgttct
       61 tttcaataat ttaatatcgt tattattagt atcagccaat caaatgtgaa taagttggct
      121 tttagaaagg ttttaccaca atgaagaaac atcttaaaac agttgccttg accctcacta
      181 cagtatcggt agtcacccac aatcaggaag tttttagttt agtcaaagag ccgattctta
      241 aacaaactca agcttcttca tcgatttctg gcgctgacta cgcagaaagt agcggtaaaa
      301 gcaagttaaa gattaatgaa acttctggcc ctgttgatga tacagtcact gacttatttt
      361 cggataaacg tactactcct gaaaaaataa aagataatct tgctaaaggt ccgagagaac
      421 aagagttaaa ggcagtaaca gagaatacag aatcagaaaa gcagatcact tctggatctc
      481 aactagaaca atcaaaagag tctctttctt taaataaaac agtgccatca acgtctaatt
      541 gggagatttg tgattttatt actaagggga atacccttgt tggtctttca aaatcaggtg
      601 ttgaaaagtt atctcaaact gatcatctcg tattgcctag tcaagcagca gatggaactc
      661 aattgataca agtagctagt tttgctttta ctccagataa aaagacggca attgcagaat
      721 ataccagtag ggctggagaa aatggggaaa taagccaact agatgtggat ggaaaagaaa
      781 ttattaacga aggtgaggtt tttaattctt atctactaaa gaaggtaaca atcccaactg
      841 gttataaaca tattggtcaa gatgcttttg tggacaataa gaatattgct gaggttaatc
      901 ttcctgaaag cctcgagact atttctgact atgcttttgc tcacctagct ttgaaacaga
      961 tcgatttgcc agataattta aaagcgattg gagaattagc tttttttgat aatcaaatta
     1021 caggtaaact ttctttgcca cgtcagttaa tgcgattagc agaacgtgct tttaaatcaa
     1081 accatatcaa aacaattgag tttagaggaa atagtctaaa agtgataggg gaagctagtt
     1141 ttcaagataa tgatctgagt caactaatgc tacctgacgg tcttgaaaaa atagaatcag
     1201 aagcttttac aggaaatcca ggagatgatc actacaataa ccgtgttgtt ttgtggacaa
     1261 aatctggaaa aaatccttat ggtcttgcta ctgaaaatac ctatgttaat cctgataagt
     1321 cactatggca ggaaagtcct gagattgatt atactaaatg gttagaggaa gattttacct
     1381 atcaaaaaaa tagtgttaca ggtttttcaa ataaaggctt acaaaaagta aaacgtaata
     1441 aaaacttaga aattccaaaa cagcacaatg gtgttactat tactgaaatt ggtgataatg
     1501 cttttcgcaa tgttgatttt caaaataaaa ctttacgtaa atatgatttg gaagaagtaa
     1561 agcttccctc aactattcgg aaaataggtg cttttgcttt tcaatctaat aacttgaaat
     1621 cttttgaagc aagtgacgat ttagaagaga ttaaagaggg agcctttatg aataatcgta
     1681 ttgaaacctt ggaattaaaa gataaattag ttactattgg tgatgcggct ttccatatta
     1741 atcatattta tgccattgtt cttccagaat ctgtacaaga aatagggcgt tcagcatttc
     1801 ggcaaaatgg tgcaaataat cttattttta tgggaagtaa ggttaagacc ttaggtgaga
     1861 tggcattttt atcaaataga cttgaacatc tggatctttc tgagcaaaaa cagttaacag
     1921 agattcctgt tcaagccttt tcagataatg ccttgaaaga agtattatta ccagcatcac
     1981 tgaaaacgat tcgagaagaa gccttcaaaa agaatcattt aaaacaactg gaagtggcat
     2041 ctgccttgtc ccatattgct tttaatgctt tagatgataa tgatggtgat gaacaatttg
     2101 acaataaagt ggttgttaaa acgcatcata attcctacgc actagcagat ggtgagcatt
     2161 ttatcgttga tccagataag ttatcttcta caatggtaga cctcgaaaag attttaaaac
     2221 taatcgaagg tttagattat tctacattac gtcagactac tcaaactcag tttagagaca
     2281 tgactactgc aggtaaagcg ttgttgtcaa aatctaacct ccgacaagga gaaaaacaaa
     2341 aattccttca agaagcacaa tttttccttg gccgcgttga tttggataaa gccatagcta
     2401 aagctgagaa ggctttagtg accaagaagg caacaaagaa tggtcagttg cttgaaagaa
     2461 gtattaacaa agcggtatta gcttataata atagcgctat taaaaaagct aatgttaagc
     2521 gcttggaaaa agagttagac ttgctaacag gattagttga gggaaaagga ccattagcgc
     2581 aagctacaat ggtacaagga gtttatttat taaatacgcc tttgccattg ccagagtatt
     2641 atatcggatt gaacgtttat tttgacaagt ctggaaaatt gatttatgca cttgatatga
     2701 gtgatactat tggcaaggga caaaaagacg cttatggtaa tcctatatta aatgttgacg
     2761 aggataatga aggttatcat gccttggcag ttgccacttt agctgattat gaggggctcg
     2821 acatcaaaac aattttaaat agtaagctta gtcaattaac atctattcgt caggtaccga
     2881 ctgcagccta tcatagagcc ggtattttcc aagctatcca aaatgcagcg gcagaagcag
     2941 aacagttatt gcctaaacca ggtacgcact ctgagaaatc aagctcaagt gaatctgcta
     3001 actctaaaga tagagcattg caatcaaacc caaaaacgaa tagaggacga cactctgcaa
     3061 tattgcctag gacagggtca aaaggcagct ttgtctatgg aatcttaggt tacactagcg
     3121 ttgctttact gtcactaata actgctataa aaaagaaaaa atattaaatt gatttaagga
     3181 gaaaaaatga taacacaaga aatgaaagat cttattaata accaattagc aatggttgcg
     3241 acggttgatg ccaaaggtca acccaatatt ggtccaaaac gttctatgcg tctctgggat
     3301 gataaaacat ttatttataa tgaaaataca gatggtcaaa ctcgtattaa cattgaagat
     3361 aatgggaaaa ttgaaattgc ctttgttgat cgtgagcgcc ttttaggtta caggtttgta
     3421 ggaactgctg aaattcaaac agaaggtgct tattacgaag cagcgaaaaa atgggcacaa
     3481 ggacgcatgg gggtaccaaa agcagttggt attattcatg tcgagcgtat ttttaattta
     3541 caatctggtg ctaatgcagg taaagaaata aacagtgaaa gtaactaaaa ttaactgtaa
     3601 cagtatttag gacccttatt ttaaacgcta aaaaacaagc atactctttt ttacagcata
     3661 gtgactatgt tgtaaaaagg ggtatgctta ttaggtgctc ttaatatttc tagataactg
     3721 cacctaataa gttttcaata gacttatagc acattagttg acttcaatta gttgacttca
     3781 attagttgac ttcaatacca gaaagagaag aacagtgatg ttggtgttcg tttaaagaag
     3841 tatcgatttc cacagtgact gagtttatat tgtacctttg acaaatcagt ctaatagatt
     3901 ttttgcaatg tttttcagtg gtagattctt tcaagcagca atgtatagtt gctctatggt
     3961 caataccatc catcgaccaa acattaagct gattaacaga cactatatga ggaagttggc
     4021 tgagttcatg gtgcaaagcg caatagtcta tcgaatcagg aacaccatct aaaaaaatgt
     4081 tagcggttgc tactaacttt ggaagagctt tagataatat aaaagaagca atggcaattg
     4141 ataataaagg atcaagaatg taccaaggtt tccattttaa gatcagtgac aatatgataa
     4201 tggctaacca cccaaggata tcttctaaaa agtgaaggct taatatttct tcattttttg
     4261 tctggttaga gtgaatgatg aagctagcaa atccattgat tataattgca aaaatagcta
     4321 aaacgaacat accgtcataa ttaacaatag taggatgcca taattttggt atcgtttcaa
     4381 tcattactaa taatgacccg ctaataagta ttagatttgt cgttagtgct cctaaaaggc
     4441 taaatcgctt atagcctagt gaaaaatttg aactctcttt tttaacagcc tttctttcta
     4501 agatagctga gataccaata gcaatggcat ctccaaaatc gtggacagca tctgctaaaa
     4561 tagcacccga aaagaataat gtcccaaaaa taaattcaat gagggaaaaa ctaagattta
     4621 atataaaaat aatggtgact tttttactta ctggcataat tttctccttt tgt
//
LOCUS       DAUGHY010000076         4655 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.076, whole genome shotgun sequence.
ACCESSION   DAUGHY010000076 DAUGHY010000000
VERSION     DAUGHY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.076"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            45..1367
                     /gene="ska"
                     /locus_tag="VH752_001572"
     CDS             45..1367
                     /gene="ska"
                     /locus_tag="VH752_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023611186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HEQ8289905.1"
                     /translation="MKNYLSFGMFALLFALTFGTVKPVQAIAGPEWLLDRPSVNNSQL
                     VVSVAGTVEGTNQEISLKFFEIDLTSRPAHGGKTEQGLSPKSKPFATNSSAMPHKLEK
                     ADLLKAIQEQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADRDDSVTLPTQPVQE
                     FLLSGHVRVRPYQPKAVHNSAERVNVNYEVSFVSETGNLDFTPSLKERYHLTTLAVGD
                     SLSSQELAAIAQFILSKEHPDYIITKRDSSIVTHDNDIFRTILPMDQEFTYHIKDREQ
                     AYKANSKTGIVEKTNNTDLISEKYYVLKKGEEPYDPFDRSHLKLFTIKYVDVDTNELL
                     KSEQLLTASERNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHNDTNRIIT
                     VYMGKRPEGENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPKDK"
     gene            complement(1465..1908)
                     /locus_tag="VH752_001573"
     CDS             complement(1465..1908)
                     /locus_tag="VH752_001573"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEQ8289906.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLLVGVGPDDNAEDLAY
                     AVRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLA
                     SQLYDSFNEQLAEFVKVERGVFGADMQVSLINDGPVTIILDTKCH"
     gene            complement(1923..4142)
                     /locus_tag="VH752_001574"
     CDS             complement(1923..4142)
                     /locus_tag="VH752_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEQ8289907.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKADVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGHDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLAFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKDMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLTHLTTVVEKIKAVPDVYSVKRTNG"
     gene            complement(4394..4504)
                     /locus_tag="VH752_001575"
                     /pseudo
     CDS             complement(4394..4504)
                     /locus_tag="VH752_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_129544913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT         1291 a         1085 c          914 g         1365 t
ORIGIN      
        1 tattctaata atggggattg aaacttaact tttaggaggt ttctatgaaa aattacttat
       61 cttttgggat gtttgcactg ctgtttgcat taacatttgg aacagtcaag cctgtccaag
      121 ctattgctgg acctgaatgg ctgctagacc gtccatctgt caacaacagc caattagttg
      181 ttagcgttgc tggtactgtt gaggggacga atcaagaaat tagccttaaa ttttttgaaa
      241 ttgacctaac atcacgacct gctcatggag gaaagacaga gcaaggctta agtccaaaat
      301 caaaaccatt tgctacaaat agtagcgcaa tgccacataa acttgaaaaa gctgacttat
      361 taaaggctat tcaagaacaa ttgatcgcta acgttcacag taacgacggc tactttgagg
      421 tcattgattt tgcaagcgat gcaaccatca ctgatcgaaa cggcaaggtc tactttgctg
      481 atcgagatga ttcggtaacc ttgccgaccc aacctgtcca agaatttttg ctaagcgggc
      541 atgtgcgcgt tagaccgtat caacctaaag ccgttcacaa ctcagctgaa cgcgttaacg
      601 tcaactatga agtgagcttt gtctccgaaa caggaaattt agactttacg ccatcgttaa
      661 aagaacgata ccatttgacc acactggcag ttggtgactc tctttcatca caagagttag
      721 cagcaattgc ccaatttatc ctatcaaaag agcatccaga ttatatcatt acaaaacgtg
      781 actcctcaat cgtcactcat gacaatgaca ttttccgtac gattttacca atggatcaag
      841 agtttactta ccatatcaaa gaccgggaac aagcttataa ggccaattct aaaacaggta
      901 ttgtagaaaa aacgaacaac actgacctta tctctgagaa atattacgtc cttaaaaaag
      961 gggaggagcc gtatgatccc tttgatcgca gtcacttgaa actgttcacc atcaaatacg
     1021 ttgatgtcga taccaacgaa ttgctaaaaa gcgagcagct cttaacagct agcgaacgta
     1081 acttagactt cagagattta tacgatcctc gtgataaggc taaactactc tacaacaatc
     1141 tcgatgcttt tggtattatg gactatacct taactggaaa agtagaggat aatcacaatg
     1201 acaccaaccg tatcataacc gtttatatgg gcaagcgacc cgaaggagag aatgctagct
     1261 atcatttagc ttatgataaa gatcgttata ccgaagaaga acgagaagtt tacagctacc
     1321 tgcgttatac agggacacct atacctgata accctaaaga caaataacca cgatcttcta
     1381 aaacgatgag attaactgac aaaaaaagca agcaacacgc tatcaacagt tgcttgcttt
     1441 tttctaatct cttagttgta gagactagtg acatttcgtg tctaaaataa tcgtaactgg
     1501 tccatcattg atgagactaa cctgcatatc tgccccaaaa acgccacgct caactttcac
     1561 aaactctgcc aattgttcat taaaactatc atacaactga ctagccaaat ctggcttagc
     1621 agcaccagta aaggcaggac ggtttccctt tttggtgtca gcataaaggg taaattgcga
     1681 cacagataag atactgccct tgatgtcttg gatagactga ttcatcttgc catcagcatc
     1741 tgaaaaaatg cgcatgttga ctatttttcg cacagcgtaa gccaaatctt ctgcattatc
     1801 atctggtccc acacctacta acaacaacaa cccttgatta atagcgccag caattttccc
     1861 atcaatagaa acagaggcct ctttgacccg ttgcaagaca agtttcatgg ttttatcctt
     1921 tcttagccat tggtccgctt cacgctataa acatctggaa ctgccttgat tttttcgaca
     1981 acagtggtca gatgcgtcag atttggaatg ccaaagctca cgtgaatatt agcaaacttc
     2041 atgtccttag tcggctgagc attgactgtc gatatgctct tggttgagtt tgataaaatt
     2101 tggagcacat cattaagcag gccactacga ttgagcccat agatatcaat ttcagcctga
     2161 taatctttac tcgaattgtc caagtcccac tcgacctcaa tcaagcgttc ttggtagcca
     2221 tcttgactct taatgttatg acagtccgat ctgtgaatcg caatgccacg ccctttggta
     2281 atgtagccgt caataggatc accaggtacg ggattacaac acttggcaat ccgcatcaag
     2341 aggcctgatg ctccttggat aatgacccca ttttcactgc gaaccttgag cacatctttg
     2401 ttttcgtgtt tgacctcacc gcccttaacc aattcttcag cttctgcttt agccttggcc
     2461 ctttcttctt cacggcgctc tttttcggtt aacttgttaa agacactgat aggactaatg
     2521 tcaccaaacc caacggctgc atagagtgat tcttcgctct tcacactgac ttttggaagg
     2581 atcgcttcaa tgcgtttttt gtcaaggtat ttattggcaa cgtagccctg ctcttgaaaa
     2641 taagacacca acaaatcacg gcctttattc actgacaatt ccttgtcttg atttttaaag
     2701 aactgacgaa ttttgttgcg agccttattg gttttgacca gttttaccca gtctcgacta
     2761 gggccaaagg aattggcatt ggtgatgatt tcaaccacat ctcctgtttt taacttggca
     2821 gtgagaggaa ccatacgccc attaaccttg gcacctgttg ctttttcacc gatttgcgta
     2881 tggatcgcat aagcaaaatc aataggacct gattcttttg gcaactcctg aacggcccct
     2941 gtcggtgtaa agacataaat ccgttcagaa aaaatgtctt ctttgaccga atccacaaag
     3001 tccactgcat caccatttga ggcatcttgc aattctacca gctctttgat ccagttcatg
     3061 ccaacggctt gctcagcttg attgacctta ccacgcacgc cttttttata agcccagtga
     3121 gcagcaaccc cgtactcagc cacttgatgc atgtccttag ttctgatttg aatctcaata
     3181 ggtccttttg gtccatacac agtggtatga atagactggt agccattagc tttaggagct
     3241 gcaatataat ccttgaagcg gcctggcatg ggacgccaaa gctcgtgaat atagccaacc
     3301 atagcataga catcgctttg cgtttccatg acacaacgaa tggcaatcag atcaaaaatc
     3361 tgatcgaaac gtttcttttt gtcccgcatt ttccgataaa tggaataaat gtgttttggt
     3421 cggccataca catctccaaa caacccttgt tgtgtcgtat aggttttgac cttactgaca
     3481 atagcctcta ccaaggcttc acgctcgcga cgtttttctt tcatcatatg ggaaattttg
     3541 taaaattcgg tttcattgag gtaacgaaaa gccaaatctt ctagttccca tttgatgcga
     3601 ctaatcccca aacgatgcgc caagggggca tagatttcca tggtttcgcg cgaaatgcgc
     3661 tcttgtttgt ccttgcgcaa atgtttgagg gtgcgcatat tatgcaggcg gtcagccaat
     3721 ttcaccaaaa tcacgcgaat atctttggac atagccatca gcattttgcg atggttttcg
     3781 gcgagttgct cctcatgaga tttgtactca actttaccta acttggtgac accatcaacg
     3841 atatcacgag catcatggcc aaagtctgct tcgatctcat ctaaggtaat gtccgtatct
     3901 tctacgacat catgtaaaaa gccacaagca actgtcacag catccagatg caaatcagcc
     3961 agaatccccg ccacctgaat cggatggacg atatagggct cgcctgactt tctcacttgg
     4021 tagaaatggg ccgctgttgc atatgctaaa gcctttgcca caaaagccac atcagccttg
     4081 gtcatatagg tggccgctaa ggcaatgact tcttctcctg ttacgttcat tatttttgcc
     4141 atttcgttcc tcatctagca tttcttttcc ctattttaac actttacaca caaaagcaaa
     4201 aggcagaatc tgctaagaaa gcgcttagtg tgaccttttc ttagaaaacg gttagtgagc
     4261 tgcttttgat atcttttggg atctctcagg tgtgagcatg ataccaaagg cctgctcccg
     4321 accaaaaata gagagaacca aggggctctc tctattatgt ttgcttatca gtaaggcctc
     4381 tcaattcaga ggattagttg ttttctttgc gttttgtaac ggctaggaca ccagctgttg
     4441 tcatcactgc tactgctgct gctgtaaaga atgggttggc ttgctcacca gttgctggta
     4501 gacgtctgtg gttgttggct acaggtgtct tagccatatt gccattgcgg cgttctggcg
     4561 tttggttagc ttctttgtca gcaggttttt ctggagctgg agttacctct ttgctctttt
     4621 ctggagcttt ttcgcctggt tgctctggca tttct
//
LOCUS       DAUGHY010000077         4511 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.077, whole genome shotgun sequence.
ACCESSION   DAUGHY010000077 DAUGHY010000000
VERSION     DAUGHY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.077"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..179
                     /locus_tag="VH752_001576"
     CDS             <1..179
                     /locus_tag="VH752_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ8289908.1"
                     /translation="DSIEHLTHQFSLYLSDSDKETYFDIPKIIERLTLKDVVTIGKAF
                     FEKADASDFTVFPK"
     gene            229..1254
                     /locus_tag="VH752_001577"
     CDS             229..1254
                     /locus_tag="VH752_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ8289909.1"
                     /translation="MSEKSLGEVLRESRVGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTISPVSQFKSDNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIVILGTIAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTKGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
     gene            1268..1810
                     /gene="pgsA"
                     /locus_tag="VH752_001578"
     CDS             1268..1810
                     /gene="pgsA"
                     /locus_tag="VH752_001578"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEQ8289910.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVASNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMPGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            1812..2651
                     /locus_tag="VH752_001579"
     CDS             1812..2651
                     /locus_tag="VH752_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ8289911.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSIIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVQMLQEEGYPVDYGYLTEKELENQLCQLI
                     SKM"
     gene            2627..3469
                     /locus_tag="VH752_001580"
     CDS             2627..3469
                     /locus_tag="VH752_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ8289912.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFELSGGQMRRVAIAGIL
                     AMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHLMDDVANYADFVYVL
                     DKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIPISSLPITLEELREV
                     LKHG"
     gene            3462..4262
                     /locus_tag="VH752_001581"
     CDS             3462..4262
                     /locus_tag="VH752_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ8289913.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTRFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTSL"
BASE COUNT         1450 a          715 c          863 g         1483 t
ORIGIN      
        1 tagactctat agaacattta acccatcagt tcagtctata cctgtctgat tctgataaag
       61 aaacatattt tgatattcca aaaattatag aaagactcac tctaaaagat gttgttacaa
      121 ttggaaaagc attttttgaa aaagctgatg cttcagactt cacagtcttc ccaaaataga
      181 tgaaaatctg ttataatagg gttactatat tatttttgag gtacctgtat gagtgaaaaa
      241 agtcttggtg aagtgttaag agaatcgcgt gttggaaaaa atatcacact agatgatata
      301 gaattgaaga cagggatttc ttcccattac ttgctggcaa tggaattgga tcagtttaaa
      361 attattccgg aagaaaaatt tgatcaattt ttaaaagaat atgctgacat tgttgagtta
      421 gatttcaata ctttaaaaag acgataccgt tatcaagtta attccaaaaa agacagtgat
      481 ataaaatctg taactgagat tgttgaagag aagttaagta agaaacgatt acaagagaag
      541 ttgttatcac atcagagcat ggggtatggt caaccgttag aagtttctaa aaacaaagaa
      601 accttagtta tccctgtaaa gactgttggt ttagatagca aatcgccact aaaaacgata
      661 tcgcctgtta gccaatttaa atcagataac aggttaattc ctaaaaaaaa tgtgtcacgt
      721 atgaggcgaa atagcactga cagtcgttct aaaacgtcac tatttcccat agttatatta
      781 ggaacgattg cttttgcaat tatcagtgtt atcattttag gtgtttggca acaattcgaa
      841 aagagtcaaa gagctaaaga agctgaagtt gctttgctta aagcctctga gcagtctact
      901 aacggtttaa aaaataattc aaatgatcgc aaaactcaag tcacaactga ggggtcgggt
      961 aattatttaa tagcgactgt tactaaatct aaagagaccg ttgatatcag tgtgtcttta
     1021 accgaggcac aaagtagttg gatttctcta acaaattcag aaattggtga aggtggtata
     1081 acattgactc aagactcgcc aacttataca gcgactttac ctgcagaaat aacagaatca
     1141 ttgttgacac ttggtgtgac aaaaggtgta tctgtaacaa ttgatggaca acctgttgat
     1201 ctatcacctt taacaagtac tgacttaagt tatatcacgt ttaagatcca ataattaagg
     1261 tagactaatg ataaaaaaag aaaatattcc taatctatta actcttgttc gaatcgcaat
     1321 gattcctttc tttcttttta ttacttcctc atctaataag gtggggtggc atatttttgc
     1381 agctgtgatt tttgcaattg ccagttttac cgattatcta gatgggtatc ttgcgcgtaa
     1441 gtggcatgta gccagtaatt ttggaaagtt tgccgatcct ctagcagata agatgcttgt
     1501 catgagtgcc tttatcatgt tagttggact tggcttagtt cctgcgtggg tgtcagctgt
     1561 tattatttgc cgagaattgg cagtaactgg tcttcgatta ctacttgttg aaaccggagg
     1621 aaaggtcctt gcagctgcta tgccgggaaa aatcaaaaca gcgacacaaa tgttgtctat
     1681 tattttatta ctttgccatt ggatattcct aggaaacgtc ttactctata ttgctctttt
     1741 tttcactatt tattctggat atgattattt taaaggagca agctttcttt ttaaggatac
     1801 gtttaaataa catgtcagct attattgaac ttaaaaaagt tacatttaat taccataaag
     1861 accaagaaaa accaacatta gatggcgtat cgtttcatgt gaaacaaggt gagtggctgt
     1921 ctatcattgg ccataatgga tctggtaaat caacgactat tcggttaatt gatggattat
     1981 tggaaccaga atcagggtct attattgttg atggagatct actaaccata actaatgttt
     2041 gggaaattcg tcataaaatt ggtatggtct ttcaaaaccc tgataatcag tttgttggag
     2101 caactgttga agatgatgtt gcttttgggc ttgaaaataa aggtattgca catgaagata
     2161 taaaagagag ggttaatcat gctttagagt tagtcggcat gcagaacttt aaagaaaaag
     2221 aaccagcccg tttatctggt ggccaaaaac agcgcgtagc tattgcaggt gcagttgcta
     2281 tgaagcctaa aattattatt ttagatgaag ctactagtat gcttgaccct aaaggacgat
     2341 tagagttaat caaaactata aaaaacatcc gtgacgacta ccagctgact gttatttcca
     2401 ttactcatga cttagatgaa gttgctctta gtgatagagt tttagtgatg aaagatggtc
     2461 aagtggaatc aacatcaaca ccagaacaat tatttgcaag aggggatgaa ctactccaat
     2521 taggtcttga tatccctttt acaacatctg ttgtacagat gcttcaagaa gaaggttatc
     2581 ctgttgacta tggatatctc acagaaaagg aattagaaaa tcagttatgt caattaatct
     2641 ccaaaatgta agttatactt atcaagcagg gactcctttt gaagggcgtg ccctttttaa
     2701 cattaattta gatattttag atggttctta tacggctttt atcggacata ctggatccgg
     2761 gaaatcgacc attatgcaat tgctaaatgg tttacacgtt ccgacaacgg gaattgtcag
     2821 cgtagataaa caagatataa cgaatcactc taaaaataaa gagattaaat ctattcgaaa
     2881 acatgtagga ttagtttttc aatttccaga aagtcaacta tttgaagaga cagttttgaa
     2941 agatgttgct tttggccctc aaaattttgg ggtctctccg gaggaagcag aagctttagc
     3001 gcgtgaaaaa ctagctcttg ttgggatttc agaaaatctt tttgaaaaga atccatttga
     3061 actctcaggg ggtcaaatgc gtcgtgttgc tattgcgggt atccttgcta tgcagccaaa
     3121 ggttttagtt ttagatgaac cgactgctgg tcttgatcct aaagggcgta aagaattaat
     3181 gactattttt aaaaagttac accaatctgg catgactatt gttttagtaa cccatctgat
     3241 ggatgatgtg gccaattacg cagattttgt atatgttctt gacaaaggta aaattatttt
     3301 atcaggaaaa cccaaaacaa tttttcaaca agtcagtttg cttgagaaaa aacagttagg
     3361 agttcctaag gtgactaaac ttgctcaaag gcttgttgat agaggaatac ctatctcttc
     3421 tttgcctatt accttagagg agctaagaga ggtactaaaa catggataag ctgattcttg
     3481 gtcgttatat tcccggagat tctctcattc atcgtcttga cccaagaagt aaattattag
     3541 cgatgattat ttatattgtc attatctttt gggctaacaa tgttgtgaca aatttgttga
     3601 tgttgacttt tactttagca gttgtttttt tgtcaaaaat caagttgtct ttctttttaa
     3661 atggggttaa accaatgatt ggtattatcc tcttcacgac tttatttcag atgtttttct
     3721 cacaaggagg aaaagtaatt tttagttggt ggttcattag cataactgat ttaggattga
     3781 gccaagctat tttaattttt atgcgtttcg ttctaattat tttcttttca acgctgttaa
     3841 cgttgacgac aacaccttta agcctatctg atgcagttga atcattatta aaacctttaa
     3901 caagatttaa agttccagct cacgagatcg gactaatgct ttctttaagt ttacgttttg
     3961 tgcctacttt aatggatgat accacgcgta ttatgaatgc acaaagggcc cgtggagttg
     4021 attttggaga gggaaattta attcaaaaag ttaaatccat tattcctatt ttaatccctc
     4081 ttttcgcttc aagctttaaa agagccgacg cattagctat tgctatggaa gctagaggtt
     4141 atcagggagg agaagggcga actaaatatc gtcaattaga ctggcagcta aaagatagtt
     4201 tagctattgg aatagtttct ttattgggtt tgctactttt tttcttgaaa acctctttgt
     4261 aatatttttt agcataaaac ttttctttat tagtaagaag tgaattgtac ctcaaaagtt
     4321 agatacaaca tctaatcatt gaggtacttt tcttcatttg aggaattgaa aaccgctatc
     4381 tcaactatat taatcatccc aacaataaca aatcgaacga aatctaaaag gactgagtcc
     4441 tgttcaatac agaactaaat tcttagttta atgatattat cccgcttttt aatgttgcta
     4501 agaagaagag t
//
LOCUS       DAUGHY010000078         4466 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.078, whole genome shotgun sequence.
ACCESSION   DAUGHY010000078 DAUGHY010000000
VERSION     DAUGHY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.078"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..322
                     /locus_tag="VH752_001582"
     CDS             <1..322
                     /locus_tag="VH752_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEQ8289914.1"
                     /translation="SLFDLELTQNDIPSEPLPQNSILHAIERLLVYIAWGNSYDFRIV
                     KNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKYIIVGPAKAMKYIFLRLM
                     EKLK"
     gene            319..2793
                     /locus_tag="VH752_001583"
     CDS             319..2793
                     /locus_tag="VH752_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEQ8289915.1"
                     /translation="MIKDTFLKTNWLNISHHITLLVFGFYFSFYSLAKELVSSTAQPV
                     NYYTHLLNVSFVGYIISLIGLSYYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLN
                     DKRFDIWSLTKNQFYQFQALPSLLIILVMATLIKILVAYFAIEKDRFGLLGYQGNTFS
                     VALILAVVPINDIHLLKLISSRFSELVRAGNSQIALLKISGLLIVLLVIFATIIYVVL
                     NALKHLKSNKLSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAI
                     TLVAILAYVITNRYWPTTFFLLILGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVT
                     SFVKKSVIVEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLFIVSCSMLIP
                     FSYEKEGKILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNY
                     SQETIASIAQKYQKLAEDINKDRKNNIADQTVIYLLSESLSDPDRVSNVTVSHDVLPN
                     IKAIKNSTTAGLMQSDSYGGGTANMEFQTLTSLPFYNFSSSVSVLYSEVFPKMAKPHT
                     ISEFYQGKNRIAMHPASANNFNRKTVYSNLGFSKFLALSGSKDKFKNIENVGLLTSDK
                     TVYNNILSLINPSESQFFSVITMQNHIPWSSDYPEEIVAEGKNFTEEENHNLTSYARL
                     LSFTDKETRAFLEKLTQINKPITVVFYGDHLPGLYPDSAFNKHIENKYLTDYFIWSNG
                     TNEKKNHPLINSSDFTAALFEHTDSKVSPYYALLTEVLNKASVDKSPDSPEVKAIQND
                     LKNVQYDVTIGKGYLLKHKTFFKISR"
     gene            2972..3667
                     /locus_tag="VH752_001584"
     CDS             2972..3667
                     /locus_tag="VH752_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ8289916.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDASDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYRYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
     gene            3669..4010
                     /locus_tag="VH752_001585"
     CDS             3669..4010
                     /locus_tag="VH752_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEQ8289917.1"
                     /translation="MTVTASLFISFIVLTFVFFLINLIKKDKLAIKYSLLWFILALLI
                     LLFTWLPNILNKMSHFLGIHSPTNMLFFLGFCLSLAIIFSLTNNISLQNDKVKRLTQE
                     VALMKKEKTND"
     gene            4003..>4466
                     /locus_tag="VH752_001586"
     CDS             4003..>4466
                     /locus_tag="VH752_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEQ8289918.1"
                     /translation="MINPSKQNQTIFLWNMLGSLSTAVISVILLMVVTRLLTSADSDI
                     YAFAYSFANMMVVVGLFQVRNYQATDINEKYSFSQYLVARLMTCLLMLAITVIYLTLT
                     KTDSYKSTIVFLVCFYRSTDAFSDLYQGMFQQHERLDIAGKSLAYRNTLIFMV"
BASE COUNT         1466 a          658 c          781 g         1561 t
ORIGIN      
        1 atcacttttt gatttagaat taactcaaaa tgatattcca agtgaacctt taccacaaaa
       61 ctctatatta catgcgattg agcggttact tgtctatata gcgtggggga attcttatga
      121 ttttaggatt gttaaaaatc cctatgagtt aacacctttt attgacaata aactgttaaa
      181 tttacgtgaa gatgaaggag ctcataccta cgttaacttt aatcaaatgg gcggtatcaa
      241 aggagcatta aaatatatta ttgttggtcc cgctaaagca atgaagtata tattcttgcg
      301 tttaatggag aaacttaaat gattaaagac acatttttaa aaaccaattg gttaaatatt
      361 agtcaccata ttacccttct tgttttcggt ttttatttca gtttttatag tttggcgaaa
      421 gaactagtaa gctccacggc acaaccggta aactattata ctcatttact aaatgtttct
      481 tttgtgggat atattatatc actgattgga ttatcttatt atttgagtcg ccaagttagt
      541 cgacagttgt ttttgaaaac tagttttatt gtgatatctt atctaattgt cagctattgg
      601 gtacaaataa cacagcacct gaatgataaa cggtttgata tctggtcatt aactaaaaat
      661 caattttatc aatttcaagc tctaccttct ttactcatta ttttagtgat ggccacttta
      721 ataaaaatat tggtagcata ttttgcaata gaaaaagata gatttgggct attaggctat
      781 caaggtaata ctttttctgt agctctgatt ttagcagttg tgccaattaa tgacatacat
      841 ctgttaaaac taataagttc tcgattttct gaattagtaa gagcaggtaa tagccaaatt
      901 gcactgttaa aaataagtgg actgttgata gttttacttg tcatatttgc aacaatcata
      961 tacgtggttt taaatgctct aaaacacctt aagtcaaata aactttcatt ttcagtagca
     1021 gctactacta gtttgttttt agcattagtt tttaactata cgtttcagta tggcgtaaaa
     1081 ggtgatgaag cattgctagg atattatgtt ttccctggag ctactctttt tcagatagta
     1141 gctattacac tagttgctat tttagcatac gtgataacga atagatattg gccaactacc
     1201 ttctttttgc ttattctggg aacaattatt tctgttgtta atgatttaaa agaatcaatg
     1261 agaagcgagc cgttattagt aactgatttt gtttggttac aagaattagg tttagtgaca
     1321 agctttgtta aaaaatcggt gattgtagaa atggttgtag gacttgctat ttgtattgtg
     1381 gtagcttggt atctacatgg ccgagtttta gcagggaaat tatttatgag ccctgtcaaa
     1441 cgggcaagtg ctgtattagg tttatttatt gtatcttgta gtatgttaat accattttct
     1501 tatgaaaaag aaggtaaaat attatctggt cttccgatta tttcggcttt aaataatgat
     1561 aatgatataa actggttagg tttttcaaca aatgctaggt acaaatcttt agcatatgtt
     1621 tggacaagac aggtgaccaa gaaaataatg gaaaaaccga caaattatag ccaagaaaca
     1681 atagcgagta tcgctcagaa gtaccaaaaa ttagcagaag atattaataa agacagaaaa
     1741 aataatattg ctgaccaaac ggttatttat cttttaagtg aaagtttgtc agatcctgat
     1801 agagtatcaa atgttactgt tagccacgat gttttaccta atatcaaggc aatcaaaaat
     1861 agcacaactg cgggactcat gcagtcagac tcctacgggg gtggaactgc taacatggag
     1921 tttcaaacgt taacaagctt acctttttat aatttttctt cttcagtatc tgttctttat
     1981 tcagaagtct ttcctaaaat ggccaaacct catacgatta gtgagtttta ccaaggaaaa
     2041 aatcgtattg cgatgcatcc tgctagtgct aacaatttta atagaaaaac agtttatagt
     2101 aatttaggtt tttccaaatt cttagctcta tcgggttcta aggataagtt taagaacatt
     2161 gaaaatgtcg gtttattgac tagcgataaa actgtctata ataatatttt atctttaatt
     2221 aatcctagtg aaagccaatt tttctcagtt attacaatgc aaaatcatat tccttggtca
     2281 tccgattatc ctgaagaaat tgttgctgaa ggaaaaaatt tcacggaaga agaaaatcac
     2341 aacctaacaa gttatgctcg gttattatcg tttactgata aggaaacaag agcattttta
     2401 gaaaaattaa cacaaattaa caagcctatc acagtggtgt tttacggaga tcatttaccc
     2461 ggtttatatc ctgatagtgc ttttaacaag catattgaaa ataaatacct tactgattat
     2521 tttatttgga gtaatggtac taacgagaaa aaaaatcatc cgcttatcaa ctcaagtgat
     2581 tttactgcag ctttatttga gcatactgat tcaaaagtat caccttacta tgctttgtta
     2641 acagaggtac tgaataaagc tagtgtcgat aaatcaccag atagtcctga agttaaagct
     2701 attcagaatg atttaaaaaa tgtccaatac gatgtgacta taggaaaagg ttaccttttg
     2761 aaacacaaaa ctttttttaa gatatcacgt taagaaaaat gctaggctgt ccgagtatag
     2821 acgagcatca aggtttggta atttgctcaa atgattgtca acagagtatc tagggccagg
     2881 tttaaaagat tataattctt aaggtcttat tcgtttataa aaaatgaata ctcttttaaa
     2941 atcttaattg aaatgaatag gagaagtttt cgtgaaaaag ttaatcatta ttcctgctta
     3001 caatgaaagc agtaatattg tcaatactat acgtactatt gaatcagatg cctcggattt
     3061 tgactatatc attattgatg attgctcaac ggataatacg ttagcaatat gtcaaaaaca
     3121 ggggttcaat gttatttctt tgcccattaa cctgggaatt ggcggtgcgg tgcaaactgg
     3181 ctatcgttat gcacaaagat gtggatatga cgttgcagtt caagtagatg gagatggtca
     3241 gcacaatcca tgttatttgg aaaaaatggt tgaggtatta gttcaatctt cagtaaatat
     3301 ggtaattgga tcacgattta tcacaaaaga agggtttcag tcatcatttg ctcgacgtat
     3361 cggcataaag tattttacat ggcttattgc gctactaaca ggaaaaaaaa taacagatgc
     3421 aacgtcaggt ctaaggttaa ttgaccgttc attgattgaa cgttttgcta atcattatcc
     3481 agatgattat cctgaacctg aaacagttgt tgatgtattg gttagtcatt ttaaagtgaa
     3541 agaaattcct gttgtaatga atgagcgaca aggcggtgtg tcatctatct cattgacgaa
     3601 atcggtctat tatatgatta aagtgacttt agctatttta gttgttagat tgaaaggaaa
     3661 tcgttaaaat gacggtaaca gccagtttat ttataagttt tattgtatta acctttgttt
     3721 tctttttgat taacttaatc aaaaaagaca aactagcgat taagtactct ttattgtggt
     3781 ttattttagc tttattaatt ttactattta cgtggttacc taacatatta aataaaatgt
     3841 ctcacttcct gggtattcat tcaccaacca atatgctatt ctttttaggt ttttgtttga
     3901 gtttagctat tattttttca ctgactaata atatctcact tcaaaatgat aaagtaaaac
     3961 gtctgaccca agaagtggcc cttatgaaaa aagagaaaac caatgattaa tccttcaaaa
     4021 caaaaccaaa cgattttttt atggaatatg ttaggaagtt tatcaacagc tgtaatatca
     4081 gttatattac ttatggtggt tactaggtta ctaacttcgg ctgattcaga tatttacgcc
     4141 tttgcctatt cttttgctaa tatgatggta gttgttggac tttttcaggt tcgtaattat
     4201 caagcaacag atattaatga aaaatattcc tttagtcagt acttagtggc aagactgatg
     4261 acttgtttat taatgttagc cataacagta atttatttaa cgttaactaa gacagatagc
     4321 tacaagagta caattgtctt tctagtctgt ttctatagat cgacagatgc tttttcggat
     4381 ttatatcagg gaatgttcca acaacatgaa cggctggata ttgcaggtaa atcgctagct
     4441 tacagaaata ctcttatttt tatggt
//
LOCUS       DAUGHY010000079         4420 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.079, whole genome shotgun sequence.
ACCESSION   DAUGHY010000079 DAUGHY010000000
VERSION     DAUGHY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.079"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            34..2307
                     /locus_tag="VH752_001587"
     CDS             34..2307
                     /locus_tag="VH752_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEQ8289919.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGNDMVDKMSKSKDLEYHFVSAKQAQEGLKEGDYYMV
                     ITLPEDLSQRAATLLNPEPQKLTIRYQTSKGHGMVAAKMGETAMAKLKESVSQNITKT
                     YTSAVFSSMTDLQSGLKEASAGSQALASGAKTAQAGSQTLSTNLAALTGASQQFQQGT
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLHPPNLNADELGNSLGAIAQAAKQVIA
                     EETAAQNEQLSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     GLQTLSQGNSLAQLDQLKAAVSQIASQSNQALPGASSALSELSMGLSKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTVIGSGVIKLSEGANALSSKSGELLDGSHQLSEGATKLADS
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            complement(2424..3497)
                     /locus_tag="VH752_001588"
     CDS             complement(2424..3497)
                     /locus_tag="VH752_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEQ8289920.1"
                     /translation="MKNISRKCFMTSVVCIVLGAILLGAGYATGGLQDIKHQTAPKKV
                     IQTFDHVTALDIDTNFSTITVETGPVKKPTVTYYTHSKFIDPLEIKIENETLHISEKP
                     RDVIITGGIELIGFGLNKSDRNHNYRTTTITLPKGTSLKEFKGSSMPQATISDLTVQD
                     LEFSGGLTLLNTRIKKGKVFGTFDATDSLLANLELKADYSLSSLTNSSLENGTISLGH
                     GQLTTKNTNLKSVTIQSLSPGGVEAEETSLENVTFTVTSEQENEETNDNGAIFTAHAL
                     TLKGTNTISGGNVAVDITLTDPKTTAYKTKSEDGKISLGSQLTPAKIGKESTSDVISY
                     VAENKAATGNLTVNLNKGDITIK"
     gene            complement(3494..4081)
                     /locus_tag="VH752_001589"
     CDS             complement(3494..4081)
                     /locus_tag="VH752_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEQ8289921.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEAMEYYIEYFDEAGPDKENQV
                     IEDLGSPKEAASEIIATVLGKHMASPEKTPEKRATIVGLAILALFAAPIALPVLLALI
                     LFIIACLMAGITAIVAAYVFGLVGVLVAGITLFESLTLLGSSLPAQAMGIGSALLSFG
                     GGILIWIIATALLRFSGRAFVAFIKWISHKKGAQA"
     gene            complement(4068..4394)
                     /locus_tag="VH752_001590"
     CDS             complement(4068..4394)
                     /locus_tag="VH752_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEQ8289922.1"
                     /translation="MYFPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLY
                     PILKKLEKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGS
                     LRHDKD"
BASE COUNT         1315 a          867 c          884 g         1354 t
ORIGIN      
        1 tttttgaaca gtatgtccat tttaggagaa aatatgttag aagaattgaa aacacttatt
       61 aaaaatccaa aattaatgat tacaatgatt ggtgtggccc tagtgcctgc cttatataat
      121 ttatcctttc taggctcaat gtgggatcct tatggtcggg tcaatgacct tcccattgct
      181 gttgttaatc atgataagcc tgcaaagaga gctgataagt cattgacaat tgggaatgat
      241 atggtggaca agatgtctaa aagtaaagat ttagagtatc attttgtctc agctaagcaa
      301 gctcaagagg gacttaaaga aggtgattat tatatggtta ttactttacc cgaagatctt
      361 tctcaacggg cagcaacctt attaaatccc gaaccccaaa aattaactat ccgttaccaa
      421 acgagtaaag gacatggaat ggtcgctgct aagatggggg aaacagcgat ggctaagctg
      481 aaagagtctg tttcgcaaaa cattacgaag acttatacct cagcagtttt tagcagtatg
      541 acagacctcc aatcaggatt aaaagaagcc tcagctggaa gtcaagcact tgcttcagga
      601 gcgaagaccg ctcaggcggg gagtcaaaca ctttcgacga acttagcagc cttaacgggt
      661 gctagccaac agtttcaaca aggtactggt cgattgacat caggtttgac tacctataca
      721 gatggtgtca accaagtcaa gaatgggtta ggaacattat caacggacat ccccaattat
      781 ctgaacgggg tttctaggtt atcccaggga gcttctcagc ttaatcaggg tctttcacag
      841 ttgacacaag caacaacact ttctgacgag aaagctaagg gaattcaatc cttaattgta
      901 ggactaccag tcctaaatca aggcattcag caactaaata cagagctatc aacattgcac
      961 ccccctaacc ttaatgctga tgagttaggt aatagcttag gagctatcgc tcaagctgcc
     1021 aaacaagtta ttgctgaaga gactgccgct cagaatgaac aactatcggc tctccaagcc
     1081 actagtgttt atcaatcatt aactgctgaa caacaaggag agttagctgc ggccctcagt
     1141 caatctgata aaagtcaaac cgtatctgca gcccaaacta ttttaagttc tgttcaaaca
     1201 ctgtcaacag gtttacaaac tctgtcccaa ggaaatagct tagctcaatt agaccagctt
     1261 aaagcagctg tttcacagat tgcgagtcag tctaaccagg ctttacctgg agcgagttct
     1321 gcattatctg agttgtcaat ggggttatca aaggtaaatg gtagcttaaa tcaacaagtt
     1381 ctaccaggaa gtaatcaatt gacaacagga ttagcacaat taaacaggta taatactgtc
     1441 attggttctg gggtaataaa actctcagaa ggtgccaatg ccttgtcatc caagtccgga
     1501 gaattactag atggtagcca tcaattatca gagggtgcta ctaaactagc tgatagtagt
     1561 tctcaattga gtcagggtgg tcatcaatta acgagcggat tgactgaatt atcaacagga
     1621 ttatcaacct taaatggttc cttagccaaa gcctctcagc agttatcgct tgtttctgtg
     1681 actgataaaa atgctaaagc tgtcgcaaaa cctcttgtgt taaatgagaa agacaaagat
     1741 ggtgttaaga cgaatgggat cgggatggca ccttatatga ttgctgtttc tctaatggtt
     1801 gtggcccttt caaccaacgt catttttgct aattctttat ctggtcgtcc ggtcaaagat
     1861 aaatgggatt gggctaaaca aaaatttgtt attaatggtt ttatttcgac tatgggatcc
     1921 attgttctct acttagctat tcaattatta gggtttgaag cccgttatgg tatggaaacc
     1981 ttaggattta ttatgctaag tggttggacg tttatggctc ttgtcacagc tttggtcggt
     2041 tgggatgatc gatatggctc ttttgcttct ttggtcatgt tattacttca ggttggctct
     2101 tcaggtggct cttaccccat tgagttaagt ggagcattct tccaaaagtt acatcctttc
     2161 ttaccaatga cttatgtggt atctggttta cgacaaacca tttcattatc aggtcatatt
     2221 ggagtagaag tgaaagtctt aactggtttc ttactggcat ttatggtatt agcactactc
     2281 atttatcgtc ccaagaaaac agtctaaata aaataacctc aaaaaaaaac agcatctaag
     2341 gcagttacac tagatgctgt tttttgtctt attttgacaa taacttgaca atttctgtca
     2401 agttttttat tgtcttgtta agatcatttg atagtaatgt ctcccttatt gagattaacc
     2461 gttaaatttc cagtagctgc tttattctca gccacataag aaataacatc tgaagttgat
     2521 tccttaccaa ttttagctgg tgtcagctgt gatccaagtg agattttacc atcttcgctt
     2581 tttgtcttat aggcggtcgt ctttggatcg gtcaaagtta tgtcaacagc aacattacca
     2641 ccactaatag tattagtgcc tttaagggta agagcatgag cagtgaatat agcaccatta
     2701 tcattggttt cctcgttttc ttgctcacta gtaacagtga aagtcacatt ttcaaggctt
     2761 gtttcttcgg cttcaacccc accaggagat agagattgga tggtgacaga tttaagatta
     2821 gtattttttg tggttaattg tccatgccct aagctaatcg tcccattttc taaactagaa
     2881 ttcgtaaggc ttgacaacga atagtcggct ttcaactcaa gattggctaa caagctatca
     2941 gtagcgtcaa atgtcccaaa aaccttccct ttctttattc tagtatttag aagtgtcaga
     3001 ccaccactga actcaaggtc ttgaaccgtt aaatccgata tagtggcttg aggcatgctt
     3061 gagcctttga actctttaag actggtacct tttggaagcg ttatggtagt ggttcggtag
     3121 ttgtggttgc ggtcactttt atttaatcca aacccgatca gttcaatacc tccggtgatg
     3181 atgacatctc tgggtttttc agaaatatga agtgtctcgt tctctatttt aatttctaaa
     3241 ggatcaataa atttagaatg tgtgtaatag gtaacggttg gttttttgac aggtcctgtt
     3301 tctactgtaa tagttgaaaa attggtatca atatcaagag ctgttacgtg atcaaatgtt
     3361 tggataacct ttttaggggc tgtttggtgt ttaatgtctt gaagcccgcc agttgcataa
     3421 ccagctccta aaagaatggc tcctaaaaca atacacacga cactggtcat aaaacatttg
     3481 cgtgagatat ttttcatgct tgagctcctt tcttgtgact aatccattta ataaaggcaa
     3541 cgaaggcacg tcctgaaaaa cgcaggagtg cagtggcaat aatccaaatg agaataccac
     3601 caccaaagct cagcaaggcg ctaccaattc ccatagcctg agcaggaaga gatgaaccaa
     3661 gtaaagtcaa actttcaaag agggtgattc ctgcaacaag tacccctaca aggccaaaaa
     3721 cataagcagc aacgatagct gttattccag ccatgaggca agcaataata aagagaatta
     3781 aagccaataa aaccggtaaa gcgataggcg cagcaaagag tgctaaaata gctagcccga
     3841 cgatagttgc tcgcttttca ggtgtttttt caggacttgc catgtgtttt cctagaacag
     3901 ttgcgatgat ttcactagcg gcctctttgg ggcttcctaa atcctcgata acttgatttt
     3961 ctttatcagg accagcttca tcaaaatatt caatataata ttccatcgct tcatggtagt
     4021 cttctctagg tagtttgcgc aagtatttat ctagttctgc tagatactca gtccttgtca
     4081 tgtcttaagc tcccttcaat aatcccgtct aaggcaaatt tatagctctg ccattctttt
     4141 tttagaaaaa tcagctgtgc tctccccgaa gaagtgacag cataatattt acgctttcgt
     4201 ccctgatgtt cttgactata agttgtcaaa aaacctgcct tctccaattt tttaagaata
     4261 ggatagaggg tggattcttt gatattagcc actagtttaa tggtttgact aatatcgtaa
     4321 ccgtaagaat catttttttc gacaatggct aaaatcagaa attcaatcaa ggtagccgat
     4381 actggaaaat acattttagg ctccttccat atataaaaat
//
LOCUS       DAUGHY010000080         4360 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.080, whole genome shotgun sequence.
ACCESSION   DAUGHY010000080 DAUGHY010000000
VERSION     DAUGHY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.080"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..193)
                     /locus_tag="VH752_001591"
     CDS             complement(<1..193)
                     /locus_tag="VH752_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEQ8289923.1"
                     /translation="MGLLDLIGRKRARDKPQNSYEGQDFFYLFGRTTSGENVDEFKGI
                     KVEFIQLSNKNIFDVLEKDL"
     gene            complement(298..570)
                     /locus_tag="VH752_001592"
                     /pseudo
     CDS             complement(298..570)
                     /locus_tag="VH752_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(589..894)
                     /locus_tag="VH752_001593"
     CDS             complement(589..894)
                     /locus_tag="VH752_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ8289924.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADNLLERLSQEM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            complement(894..1196)
                     /locus_tag="VH752_001594"
     CDS             complement(894..1196)
                     /locus_tag="VH752_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEQ8289925.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLISSFEDAKTGNRKDVREFLSQLKENA"
     gene            complement(1251..1427)
                     /locus_tag="VH752_001595"
     CDS             complement(1251..1427)
                     /locus_tag="VH752_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEQ8289926.1"
                     /translation="MGQNVVMRQAPGGNIKPDKEKSVEKIDGIVGLILGLDRCIRRQG
                     DEASVYDERGILSF"
     gene            1443..2336
                     /locus_tag="VH752_001596"
     CDS             1443..2336
                     /locus_tag="VH752_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289927.1"
                     /translation="MTTMLDFPFHHEFIEFLGRWTHILITLLKRHHGQADTAIPRYLL
                     VTIREKTGIVKSTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCACPGCDRPLILTSGN
                     LAAENYEVSAIEKDKDAEPLNLIALCPDCFLSYQADSRKKIVTALKNVKKILVSAHNS
                     QQAISDMKLDSGIVSVLTSLNKLKCDEYDISYDSKQLTDKISPENNRTLYQLVKNPVI
                     DNYLTIQKIIVNLDKQGKIDYEEIQYQTRSMYKKLKAAKHNNLAIFNTISEKLHKATL
                     QDIYFCQTIVSYFIQKCEVLE"
     gene            2336..2560
                     /locus_tag="VH752_001597"
     CDS             2336..2560
                     /locus_tag="VH752_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289928.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLNEIKSGNSNVKDIFHAISEELD
                     DPTDFLSIMDCLYALNAIEMSNEGEVKLCL"
     gene            2551..3723
                     /locus_tag="VH752_001598"
     CDS             2551..3723
                     /locus_tag="VH752_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEQ8289929.1"
                     /translation="MLIEMWSPVFKNHGEIRESICFHYGLNVIMGMDLADNSIGKSSS
                     LLAIDFIFGGNSYLKSIAVKKLGDHPIYFCFQFEKKFYFSGDTANPDIILVCDKSYSP
                     TGETMDLGIFLNKLKKRYHLDSPELSFRLAMSGFFRIAGKSNQNTDFPLQVYSSQKSS
                     ESITTLIQLFNLYDNIARYKERLKDKSDQLTTFRNARKYTFISNLVGGKKQFEANVSE
                     IKRLEYDLAHLQDTHQDKIDSDDIEKNQQKLQLRNTKLELESSLRDKQRRLKLLDISI
                     EFGLYPTESDLTELQQYFPDTNLKKLYEVEAYHKKLETILDSEFSTERESLIAEIDEL
                     ESQLTTLNQELQELGNIPNLSSEYLENYSKLTATINALKEQNEAYLKESGLSKEKS"
     gene            3736..4209
                     /locus_tag="VH752_001599"
     CDS             3736..4209
                     /locus_tag="VH752_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HEQ8289930.1"
                     /translation="MKRSTEDILLDLEVKINSKMREVNNILYPDIRKAPQINLNAHNS
                     YSFYTPDDDGTGTKFKGMIVFDLVMLYLTNLPALAHDSLLLSNVSYQATEALLKLYDQ
                     SRSLNKQVFLAFDKASSYSPDANQLLSENTVLRLSSNGNELYGISWNKGENSDEI"
     gene            4199..>4360
                     /locus_tag="VH752_001600"
     CDS             4199..>4360
                     /locus_tag="VH752_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ8289931.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIAL"
BASE COUNT         1459 a          898 c          725 g         1278 t
ORIGIN      
        1 ataaatcttt ctccaaaaca tcaaatatat ttttgttaga cagttgaata aattctactt
       61 taattccttt aaactcatcc acattctcgc cactagtcgt gcgaccaaag aggtagaaaa
      121 agtcctgacc ttcataactg ttttgaggct tatctctagc cctttttctt ccaattaaat
      181 caagtagtcc cataagccct ccttattttg gggtacaaaa aaagcacctc ctttgaagtg
      241 ctttcggtat atttctttca aagtaatcaa cagtctttct tacaaaacct agtattctca
      301 ttctgtcaat tccttaaatg cgtcaagatg tcgtgataat actgaagtcg caatctcatc
      361 taaccttgct tgttcaacaa ctgttgtcat ctgctcatca ctgcttaaac tttggtgatc
      421 cgctgatagt ttgctatcag atggacttaa taccaaagtt ccatcagttg atttataaag
      481 gttaaactcc taattctctt gaatgccaaa tgggtcaggt atcggaagat agatgtcatc
      541 accaatttgt atcgtcttta cgagttccat acatcactct cccttacttt accaattatt
      601 catatgacgt gatccataga cgaccgcaac aattataact tcatcttcca gaacatggta
      661 tacaatgcga tattttttga caatcagttg tctgaaggta tagccttttc caatcagatc
      721 ctcaatgatg gcacaacgtt caggaaaaat ggatagtgat aacatttctt gggataacct
      781 ctcaagaaga ttatccgctg cctgtggtgc acagagttca tcacgaacat agtgatagat
      841 gctcagcaaa tctgctttag catcatccga catggtgacc tgatattctt tcattaagca
      901 ttttccttca attgagatag aaattctctg acatccttgc ggttgccagt tttcgcatct
      961 tcaaagctgg agataagctt atcataaaac ttctcctgac tcatgtaatc aacattaact
     1021 cgttgaggcg cttcaggtag agaaacatca aatgggatac ccccagtcaa aataatctga
     1081 ttcaaaaaca tatcaatcgc agttgacata ggaataccta agcgtttcaa aatatcatct
     1141 gccgcacttt ttactgaatc atcaactcgt aaattcaaag ttcccgtttt agccatggtt
     1201 atcctccttt tttgtaacga cattgtatca cgtttttaga gttgactcaa ctaaaaactc
     1261 aatatccctc gctcatcata gacgctagct tcatcacctt ggcggcgaat gcaacggtca
     1321 agccccagga taagacctac aataccgtca atcttctcaa cagatttttc cttatctggc
     1381 ttgatgttgc ctccaggggc ttgtcgcatc accacgtttt gtcccatcca tttaaggact
     1441 ggatgaccac catgttagat tttcccttcc atcatgagtt tatagagttc cttggacggt
     1501 ggactcatat ccttataact ctgctcaaaa ggcaccatgg tcaagccgac accgcaattc
     1561 caaggtatct tctagtaacc ataagagaga agacagggat tgtaaaatca accactgtgc
     1621 aaaatactgc gttcactacc cagtcgaatc agctcaaaaa acaatacggg cagttcttgt
     1681 taacagaatc taataactgt tgtgcatgcc caggctgtga tagaccactc attctaacca
     1741 gtggcaactt agcagcagaa aattacgagg tttcagccat tgaaaaagat aaggacgctg
     1801 aaccactgaa tctgatagcc ctctgcccag attgtttttt atcctatcaa gcagatagtc
     1861 gcaaaaaaat tgtaacagct ctaaaaaatg ttaaaaagat tctagtctct gcacataaca
     1921 gccaacaagc gatttctgat atgaagttag acagtggaat tgtttcagtt ttgactagtc
     1981 tcaataaact aaaatgtgat gaatatgata tctcctatga ctcgaagcaa ttgactgata
     2041 agatatcacc tgagaacaat cgtacgcttt atcaattggt taaaaaccca gttattgata
     2101 actacttaac cattcaaaaa atcatcgtaa atttggacaa acaaggaaaa attgattacg
     2161 aggaaatcca atatcaaacg cgatcgatgt ataaaaagtt aaaagcagcc aagcataata
     2221 atctggctat tttcaatact atttcggaaa aactccataa agctacacta caggacatct
     2281 acttctgtca gacgattgtc tcttacttta tccaaaaatg cgaggtactt gaataatgca
     2341 attaccaaat aaactttact cctatcaaaa aagcaccctt gcttatttgc ctagagtgct
     2401 aaatgaaatc aaaagtggaa actcaaacgt caaagatatc tttcacgcta tctcagaaga
     2461 gttggatgat cctacagatt tcctatctat aatggattgc ttgtatgcat tgaacgctat
     2521 tgaaatgtct aatgaaggag aagtaaaatt atgcttatag aaatgtggtc tccagtattt
     2581 aagaatcacg gagaaattcg ggaatcaatc tgcttccact atgggttaaa cgtgattatg
     2641 gggatggatt tagctgataa ctctatcggc aaatcctcct cactattagc cattgatttc
     2701 atctttggag gtaatagcta tctaaaatca attgcggtta aaaaattagg tgatcatcct
     2761 atctatttct gctttcaatt tgagaaaaag ttttattttt caggagatac ggccaaccca
     2821 gatatcattc tagtgtgtga caagagctac tctccaaccg gtgaaacaat ggacctagga
     2881 atttttctta acaaacttaa gaaacgatat catttagatt ctccagagct atcattccga
     2941 ttagctatga gtggcttctt taggattgca ggaaaaagta atcaaaatac cgatttccct
     3001 ttacaagttt attcgagcca aaaatcttct gaatctatta ctactctcat ccaactgttt
     3061 aatttgtatg ataacattgc acgatacaaa gaacgtctga aagataagag cgaccagtta
     3121 acaacttttc gtaatgctcg aaagtatacc ttcatctcca acctggttgg cggtaaaaag
     3181 caatttgaag caaatgtctc agaaataaaa agattggaat atgatttagc acatctccaa
     3241 gacactcatc aagataaaat tgactctgat gatattgaga aaaatcaaca aaaactccag
     3301 cttagaaata ctaagctgga gttagaaagc agtcttcggg acaagcaaag acgtcttaag
     3361 ctattggaca tcagtatcga atttggctta tatcctactg aatctgactt aacagagctt
     3421 caacaatact tcccagatac taatctcaaa aaattatatg aagttgaagc ttaccacaaa
     3481 aaattggaaa ctattttaga ttccgaattc tcaactgaac gtgaatccct aatagctgag
     3541 atcgacgaat tagagagtca attaacaact ctaaaccaag aacttcaaga actaggaaac
     3601 atcccaaatc tttcaagtga gtacttagaa aactactcta aactaacagc tacaatcaat
     3661 gcactaaaag aacaaaacga ggcctactta aaggagtctg gtctatccaa agaaaaatca
     3721 taagcggacg ctgacttgaa acgcagtaca gaagatatcc ttctagatct agaagtaaaa
     3781 atcaacagta agatgcgtga agtcaataac attctatatc cagatattcg caaagctcct
     3841 caaattaacc taaatgctca caatagctac tccttctata caccagatga tgatggtaca
     3901 ggaactaagt ttaaagggat gattgtattc gacttagtaa tgctctacct tactaatctg
     3961 ccagcactcg cacacgattc attactcttg agtaatgtta gctaccaagc gactgaagca
     4021 cttttgaagc tgtatgacca gtcaaggtca ttgaacaaac aagtcttttt agccttcgat
     4081 aaagccagct cttattctcc agatgctaat caactcttgt cagaaaatac ggtattgaga
     4141 ctctctagta acggaaatga actctacggt atttcatgga acaaaggaga aaactcagat
     4201 gagatttagt tacaataaat tatggaaact tcttattgat aaaggttgga ccaaaacaaa
     4261 gctcaggcaa gaagctggga ttagttcttc cacgatcgca aaattgggaa aaggggaaaa
     4321 tatcactact gatattttat tggaaatttg tattgcactt
//
LOCUS       DAUGHY010000081         4249 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.081, whole genome shotgun sequence.
ACCESSION   DAUGHY010000081 DAUGHY010000000
VERSION     DAUGHY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.081"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            29..571
                     /gene="hpt"
                     /locus_tag="VH752_001601"
     CDS             29..571
                     /gene="hpt"
                     /locus_tag="VH752_001601"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEQ8289932.1"
                     /translation="MLEQDIQKILYSENDIIRKTKKLGEQLTKDYQEKNPLMIGVLKG
                     SVPFMAELMKHIDTHVEIDFMVVSSYHGGTSSSGEVKILKDVDTNIEGRDIIIVEDII
                     DTGRTLKYLRDMFKYRKANTIKIATLFDKPEGRVVKIEADYVCYNIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEVYSK"
     gene            593..2572
                     /gene="ftsH"
                     /locus_tag="VH752_001602"
     CDS             593..2572
                     /gene="ftsH"
                     /locus_tag="VH752_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEQ8289933.1"
                     /translation="MKNNKNNGFVKNSFIYILMIIVVITGFQFYLKGTSTQSQQISYS
                     KLIKHLKAGDIKSLSYQPSGSIIEVKGKYEKPKKVTVNSGLSFLGGRASTQVTEFSSL
                     VLPSDTILKEMTAAADKNGTELTVKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGG
                     GGARGAMSFGKNKAKSQSKGDVKVRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFV
                     GADLENVLNEAALVAARRNKIKIDASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGR
                     VAEEIVFNAQTSGASNDFEQATQIARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEK
                     AYSAQTAQMIDDEVRELLNQARNQAADIINENRDTHKLIAEALLKYETLDAAQIKSIY
                     ETGKMPVDLETDDNEAHALSYDEIKNKMIESESAE"
     gene            2899..>4249
                     /locus_tag="VH752_001603"
     CDS             2899..>4249
                     /locus_tag="VH752_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ8289934.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLVFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAIAIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPMLAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     "
BASE COUNT         1397 a          617 c          839 g         1396 t
ORIGIN      
        1 attgataaat tagaaaaagg gtgacagcat gctcgagcaa gatattcaaa aaattcttta
       61 ctctgaaaat gatattattc ggaaaactaa aaaattagga gaacaattaa caaaagatta
      121 tcaagaaaaa aatccgctta tgattggtgt attaaaagga tcagttcctt ttatggcaga
      181 actaatgaaa cacatcgata ctcatgttga aattgatttt atggtagtat caagttatca
      241 tggcggtaca tctagcagtg gtgaagttaa aattttaaaa gatgtagata ccaatattga
      301 aggaagagat atcataattg ttgaagatat tattgacacc ggtcgcacct taaaatattt
      361 acgagatatg tttaagtacc gtaaagctaa tactatcaaa attgcaacac tgtttgataa
      421 acctgaaggt cgtgttgtta aaattgaagc agattatgtt tgttacaata ttccaaatga
      481 atttatcgta ggctttggtt tagattacgc agaaaattat cgcaatcttc cttacgtagg
      541 tgtattaaaa gaagaagtct attcaaaata gaaaaaggtc aatgtttact gtatgaaaaa
      601 taataaaaat aatggttttg ttaaaaattc ttttatatat atattgatga ttattgttgt
      661 catcacagga tttcaatttt atcttaaggg aaccagcaca caaagccaac agattagtta
      721 ttccaaatta attaaacatc ttaaggctgg agatataaaa tctttaagtt atcagcctag
      781 cggtagtatt attgaagtta aagggaaata tgagaaacct aaaaaagtga cagttaattc
      841 aggactatca tttttaggtg gaagagcttc aacacaggta acagagttct catctttagt
      901 attacctagt gataccatct tgaaagaaat gacagcagca gctgataaaa atggaacaga
      961 gcttacagtt aagcaggaaa gttcaagtgg tacttggatt accttcttaa tgagcttttt
     1021 acctattgtt atttttgctg ctttcatgat gatgatgatg aatcaaggtg gtggaggagc
     1081 tcgtggtgct atgagtttcg gcaaaaataa ggcaaaatct caatcaaaag gtgatgttaa
     1141 agtgagattt acagacgttg ctggtgccga agaagaaaaa caagaattag tagaagttgt
     1201 tgattttttg aaaaatccta aaaaatataa atcactagga gcacggattc cagcgggagt
     1261 tcttctagag gggcctccag gtactggtaa aacattacta gctaaagctg tagcaggtga
     1321 agctggagtg ccatttttca gtatttcagg ttcagatttt gtcgaaatgt ttgtcggtgt
     1381 aggtgctagt cgtgttcgtt ctttatttga ggatgctaaa aaagctgagc gtgcgatcat
     1441 ctttatcgat gaaattgacg ctgtaggtcg tcgtcgtggt gctggcatgg gaggcggtaa
     1501 cgatgaacga gaacagaccc ttaaccaatt actcattgaa atggatggtt ttgagggtaa
     1561 tgagaacatt atcgttattg ctgcaactaa ccgtagtgat gttttagatc cagctttact
     1621 aagaccaggt cgttttgatc gtaaggtttt agttggtcgc cctgatgtta aaggacgtga
     1681 agccatttta cgtgttcatg ctaaaaataa accacttgca aatgacgtta atttgaaagt
     1741 agtagcccaa cagactccag gttttgtagg tgctgatctt gaaaatgttt taaatgaagc
     1801 tgcgttggtt gctgctcgca gaaataaaat taaaattgat gctagtgata ttgatgaagc
     1861 tgaagatcgt gttattgctg gaccatcaaa gaaagatcgc actatttcgc aaaaagagcg
     1921 agaaatggtt gcttatcacg aagctggtca tactattgtt ggtttagttt tatctaatgc
     1981 gcgtgtagtt cacaaggtaa ctattgtacc tcgtggacga gcaggtggtt atatgatcgc
     2041 tcttccaaaa gaagatcaaa tgcttctttc taaagaagat ctgaaagagc aattagcagg
     2101 attgatgggt ggtcgtgttg ctgaagaaat tgtttttaat gctcaaacat caggtgcgtc
     2161 aaatgacttt gagcaagcta ctcagatagc aagggccatg gttacggaat atggtatgag
     2221 tgaaaagctt ggaccagttc aatatgaagg taaccatgcg atgatgccag gacagatctc
     2281 tcctgaaaaa gcttactctg cccaaacagc tcaaatgatt gatgatgagg ttcgtgaact
     2341 cttaaatcaa gctcgtaatc aagcagctga tatcatcaat gagaatcgag atactcataa
     2401 attgattgct gaagctcttc tcaaatatga aactctagac gctgcacaaa ttaaatcaat
     2461 ttatgaaact ggtaaaatgc cagttgattt agaaactgat gataatgaag ctcatgcgtt
     2521 atcttatgac gaaattaaaa ataaaatgat agaatctgaa tctgcagaat gatataaaaa
     2581 agctgaactt atagattaag ttcagctttt ttatcaacac aatatccttg taaacgttat
     2641 gaattgtaat aggtgcctaa ttgctagaaa tgagaagatg atatactttt tttctttatt
     2701 acttaaagtg agaaagactt tggtatttaa aactagaaaa ttgttaaagc catttgaaaa
     2761 aatctattag tgttttctta taataaatag aagaacagaa gatagcaaaa gtttcagtca
     2821 accaataaga tgaaatttac aatcattact aactatgata cacttagtag aaaatcattt
     2881 actatagaag gtgataacat gactattttt cgtaaaaaaa agaaatattc caataaaaca
     2941 gaaatgcaaa ggcacttcaa agttattgat ttagtttttt taggcctagg ttcaatggtt
     3001 ggaacaggta tctttacagt tactggcata ggcgctgcta agtatgcagg tcctgcttta
     3061 acaatatcga ttatcatttc cgcgattgct attggaattt tagctttatt ttatgcagaa
     3121 tttgcgtctc gtatgccttc taacggtggt gcttatagtt atgtttatgc aaccttggga
     3181 gagtttccag catggcttgt tggctggtat attattatgg aatttttaac agctatatct
     3241 agtgttgctg ttggctgggg aagttattta aaaggattac ttgccaatta taatatagta
     3301 ttaccaaacg ctttaaatgg cacgttcaat cttaagaatg gaacttacat agatattctt
     3361 cctgttttag tgatgttttt cgtgacaggg attgtcctaa tgaattcgaa attagccctg
     3421 agattcaata gttttttagt tattttaaaa ttttcagcat tggctttatt tatctttgta
     3481 ggcatttttt ttattgatca caataattgg tctcattttg ctccatatgg tgtaggacaa
     3541 attactggag gtaaaacagg tatctttgct ggtgcttctg ttatgttctt tgcattttta
     3601 ggatttgaat ctatttcaat ggcagttgat gaagtgaaag aaccacaaaa aacaattcct
     3661 aaaggtatca ttttatcttt gattattgta acagcactct atatagttgt caccactatt
     3721 ttaacgggaa ttgtacacta cactaaatta aatgttccag acgcggtggc ttttgcttta
     3781 agaaatatta gactttattg ggcagctgac tatgtgtcga tagttgctat tttgactctt
     3841 attacggtat gtatttctat gacctatgca ttagctagaa caatttacag tattagtaga
     3901 gatggtttat tacctaaatc actttatact ctcactaaaa aaaataaagt gcctcaaaat
     3961 gctactcttg tcactggact acttgctatg atttgtgcgg gaatatttcc tttatcgagt
     4021 ctagcagagt ttgttaatat ttgtacttta gcatatttaa ttattctgtc gggtgcaatt
     4081 attaaactca gaagaataga aggagaacca aaagcaaatg aatttaaaac tcctctagta
     4141 ccattcctac caatgttggc aattatcatt tgtctatcat ttatgagcca atataaagct
     4201 tttacttgga ttgcttttgc tattgccaca attattggga cattaatct
//
LOCUS       DAUGHY010000082         4146 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.082, whole genome shotgun sequence.
ACCESSION   DAUGHY010000082 DAUGHY010000000
VERSION     DAUGHY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.082"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            61..1284
                     /locus_tag="VH752_001604"
     CDS             61..1284
                     /locus_tag="VH752_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEQ8289935.1"
                     /translation="MPSMKHILKSLSILLIGFLGGLIAIITFNNLYPHSPSKINSGKA
                     TTSNMVFNNTTNTTKAVKAVQNAVVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAE
                     LSIFSEGSGVIYRKDGNSAYVVTNNHVIDGAKRIEILMADGSKVVGELVGADTYSDLA
                     VVKISSDKIKTVAEFADSTKLNVGEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKN
                     ENGETVSTNAIQTDAAINPGNSGGPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGI
                     GFAIPSTDVIKIIKQLETNGEVIRPALGISMVNLNDLSTNALSQINIPTSVTGGIVVA
                     EVKEGMPASGKLAQYDVITEIDGKTVNSISDLQSSLYGHDINDTIKVTFYRGTTKKKA
                     DIKLTKTTQDLTKTQ"
     gene            1343..2149
                     /locus_tag="VH752_001605"
     CDS             1343..2149
                     /locus_tag="VH752_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEQ8289936.1"
                     /translation="MFLILSRNSLMTKELLIDLPIEDIVTNPYQPRIQFNQRELQDLA
                     TSIKSNGLIQPIIVRKSDIFGYELVAGERRLKASKMAGLKKVPAIIKKISTLESMQQA
                     IVENLQRSNLNAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPII
                     KAIEEGKISAGHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKT
                     KNIFATSLEKQLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLK"
     gene            2382..3737
                     /gene="dnaA"
                     /locus_tag="VH752_001606"
     CDS             2382..3737
                     /gene="dnaA"
                     /locus_tag="VH752_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEQ8289937.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNSKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNNNKQIVLTSDRTPDHLNDLEDRLVTRFKWGLTVNI
                     TPPDFETRVAILTNKIQEYNFIFPQDTIEYLAGQFDSNVRDLEGALKDISLVANFKQI
                     DTITVDIAAEAIRARKQDGPKMTVIPIEEIQAQVGKFYGVTVKEIKATKRTQNIVLAR
                     QVAMFLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMISQDESLRIEIETIKNKI
                     K"
     gene            3892..>4146
                     /locus_tag="VH752_001607"
     CDS             3892..>4146
                     /locus_tag="VH752_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEQ8289938.1"
                     /translation="MIQFSINRTLFIHALNATKRAISTKNAIPILSSIKIEVTSTGVT
                     LTGSNGQISIENTIPVSNENAGLLITSPGAILLEASFFINI"
BASE COUNT         1474 a          710 c          683 g         1279 t
ORIGIN      
        1 tttaaggtat aattaagcaa agttctataa gctatggaca aataattcgg aggacaaaac
       61 atgccaagta tgaaacatat cttaaaatcc ttaagtattt tactaattgg atttttagga
      121 ggattaatag caattattac attcaataat ctctacccac attctccatc aaaaataaat
      181 tcaggtaagg caacaactag taatatggtt tttaacaaca caaccaatac tactaaagct
      241 gtcaaagctg ttcaaaatgc agttgtatca gttattaatt atcaagataa cccttcttca
      301 tccctttcta acccttatac aaaactcttt ggagaagggc gttcaaaaga gaataaggat
      361 gctgaattat ctatttttag tgaaggatct ggggtcattt atcgaaaaga tggcaactcc
      421 gcttacgttg ttactaataa ccatgttatc gacggagcta aacgaattga aattcttatg
      481 gcagacggat ctaaagttgt tggtgaatta gttggagctg atacttattc ggatttagct
      541 gttgttaaga tctcttcaga taagataaaa acagtagctg aatttgcaga ttctacaaaa
      601 ctaaatgttg gagaagttgc tattgctatc ggcagcccac taggaacaca atacgctaat
      661 tctgttactc aaggaatcgt ctctagttta agtcgtactg taactttaaa aaacgagaat
      721 ggtgagactg tctcaacaaa tgctattcag acagatgctg ctattaaccc tggaaactct
      781 ggtggaccac taattaatat tgaggggcaa gtaatcggaa ttaattcaag taaaatttct
      841 tctaccccaa ctggtagcaa cggtaatagt ggtgctgttg aaggaattgg ttttgctatc
      901 ccatctactg atgttattaa aattattaaa caactagaaa ctaatggtga agttatcaga
      961 cctgctcttg gaatatccat ggtcaattta aatgacttat ccacaaatgc tcttagtcaa
     1021 attaatattc caactagtgt aactggtggt attgtagtag cagaagttaa ggaaggaatg
     1081 ccggcatctg gcaaacttgc tcagtacgat gtgattacag aaattgatgg caaaacagtt
     1141 aattcaatta gtgatttaca aagtagtcta tacggacacg atattaatga tactattaaa
     1201 gtaacttttt atagaggaac tacaaagaaa aaagcagata tcaaattaac aaaaactact
     1261 caagatttga ctaaaacgca gtaaacaagc tacctaaata ctattacatt ttatcacata
     1321 aaacagtcca aaggactgtt ttatgtttct tattctatcc aggaactccc ttatgacaaa
     1381 agaattattg atagacctac caattgaaga tattgttaca aatccctatc agccaaggat
     1441 acaatttaat caaagagaac tccaagacct agcaacttct attaaatcaa acggcctcat
     1501 tcaacctatt attgttcgaa aatctgacat ttttggctat gagttagttg ctggagaacg
     1561 acgacttaaa gcttcaaaaa tggctggtct aaaaaaagtt ccagctatca tcaagaagat
     1621 ctccacactc gagagcatgc aacaagctat agttgaaaat ttacaacgtt ctaaccttaa
     1681 cgctatcgaa gaagctaaag cctatcagtt attggttgaa aaaaaacaca tgactcacga
     1741 tgagattgct aaatatatgg gaaaatcaag accttatatt agcaatacct tacgtctgtt
     1801 acaactccca gcacctatca ttaaagcaat tgaagaagga aaaattagtg cgggacacgc
     1861 gcgtgctctt ttaactttga gtgatgataa gcaacaactg tacctcactc ataaaataca
     1921 aaatgaaggc ctaagtgtta ggcaaattga gcaactggtt acttctactc caagttcgaa
     1981 gctatctaaa aaaactaaaa atatttttgc cacttcttta gagaaacaat tggctaaatc
     2041 attgggactc tctgtcaata tgaagctgac agcaaaccat agtgggtacc ttcagatatc
     2101 tttttccaat gatgatgaat taaacagaat tatcaacaag ctaaagtagc ttgttgatat
     2161 tctgtttttt cttttttagt tttccacatg aaaaatagtt gaaaacaata gcggtgtcac
     2221 cttaaaatgg cttttccaca ggttgtggag aacccaaatt aacagtgtta atttattttc
     2281 cacaggttgt ggaaaaacta actattatcc atcgttctgt ggaaaactag aatagtttat
     2341 gatagaatag ttctagaatt atccacaaga aggaacctag tatgactgaa aatgaacaaa
     2401 ttttttggaa cagggtcttg gaattagctc agagtcaatt aaaacaggca acttatgaat
     2461 tttttgttca tgatgcccgt ctattaaagg tcgataagca tattgcaact atttacttag
     2521 atcaaatgaa agaactcttt tgggaaaaaa atcttaaaga tgttattctt actgctggtt
     2581 ttgaagttta taacgctcaa atttctgttg actatgtttt cgaagaagac ctaatgattg
     2641 agcaaaatca gaccaaaatc aatcaaaaac ctaagcagca agccttaaat tctttgccta
     2701 ctgttacttc agatttaaac tcgaaatata gttttgaaaa ctttattcaa ggagatgaaa
     2761 atcgttgggc tgttgctgct tcaatagcag tagctaatac tcctggaact acctataatc
     2821 ctttgtttat ttggggtggc cctgggcttg ggaaaaccca tttattaaat gctattggta
     2881 attctgtact attagaaaat ccaaatgctc gaattaaata tatcacagct gaaaacttta
     2941 ttaatgagtt tgttatccat attcgccttg ataccatgga tgaattgaaa gaaaaatttc
     3001 gtaatttaga tttactcctt attgatgata tccaatcttt agctaaaaaa acgctctctg
     3061 gaacacaaga agagttcttt aatactttta atgcacttca taataataac aaacaaattg
     3121 tcctaacaag cgaccgtaca ccagatcatc tcaatgattt agaagatcga ttagttactc
     3181 gttttaaatg gggattaaca gtcaatatca cacctcctga ttttgaaaca cgagtggcta
     3241 ttttgacaaa taaaattcaa gaatataact ttatttttcc tcaagatacc attgagtatt
     3301 tggctggtca atttgattct aatgtcagag atttagaagg tgccttaaaa gatattagtc
     3361 tggttgctaa tttcaaacaa attgacacga ttactgttga cattgctgcc gaagctattc
     3421 gcgccagaaa gcaagatgga cctaaaatga cagttattcc catcgaagaa attcaagcgc
     3481 aagttggaaa attttacggt gttaccgtca aagaaattaa agctactaaa cgaacacaaa
     3541 atattgtttt agcaagacaa gtagctatgt ttttagcacg tgaaatgaca gataacagtc
     3601 ttcctaaaat tggaaaagaa tttggtggca gagaccattc aacagtactc catgcctata
     3661 ataaaatcaa aaacatgatc agccaggacg aaagccttag gatcgaaatt gaaaccataa
     3721 aaaacaaaat taaataacat gtggaaaaga atatctttta tgaaatagtt atccacaagt
     3781 tgtgaacaac catttagtct tggattctct cgtttattta gagttatcca ctatatacac
     3841 aagacctact actactactt attattatac ttattaaata aaggagttct catgattcaa
     3901 ttttcaatta atcgcacatt atttattcat gctttaaatg caactaaacg tgctattagc
     3961 actaaaaatg ccattcctat tctttcatca ataaaaattg aagtcacttc tacaggagtc
     4021 actttaacag ggtctaacgg tcaaatatca attgaaaaca ctattcctgt aagtaatgaa
     4081 aatgctggtt tgctaattac ctctccagga gctattttat tagaagctag tttttttatt
     4141 aatatt
//
LOCUS       DAUGHY010000083         4085 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.083, whole genome shotgun sequence.
ACCESSION   DAUGHY010000083 DAUGHY010000000
VERSION     DAUGHY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.083"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            2..592
                     /locus_tag="VH752_001608"
     CDS             2..592
                     /locus_tag="VH752_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEQ8289939.1"
                     /translation="MILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQFLGSVSLIF
                     LSLLFAFVLNYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKDGLRKDNKNLIFL
                     VAMITFASCGADNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFSAQKLAQVPSVGE
                     TLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            604..942
                     /gene="cadX"
                     /locus_tag="VH752_001609"
     CDS             604..942
                     /gene="cadX"
                     /locus_tag="VH752_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEQ8289940.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDSRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            993..1916
                     /locus_tag="VH752_001610"
     CDS             993..1916
                     /locus_tag="VH752_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8289941.1"
                     /translation="MKFFEENYSQEIPTRIKNLRKKHNITQSELGNAGQVSQVESGKR
                     PITSSMLVYLNALTASSYTYIVFGELDEFIENLFHYFFSSILYRDLEAVDENLYSFMS
                     DDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKDDIDVWVGGKSYNPARS
                     FRNNPINELTVIDFEEMADILLLMLRDNLIRSFEINVCNTLFELDKNGEPTTFNLDKI
                     DSIINKWWSENVSTEIIPNLIKKLRENPLFNIGFMVNDILERMYKEDIPKSYLTSVPL
                     VISKKARSTLSIKSNGSQKIDKSKFAQILSL"
     gene            1982..2734
                     /locus_tag="VH752_001611"
     CDS             1982..2734
                     /locus_tag="VH752_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ8289942.1"
                     /translation="MAIPDLDKFSSNLQQGKIPVIKHFELNLDKGSHWAIAGNSGSGK
                     PYALTYFLSVLKPMSGLIIIDPKFDTPSQWARENKIAVIHPVENHSKSDFVSQVNEQL
                     NQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQIALLG
                     HATKIHLFLGSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPTG
                     HGTGIIQVVDNEHSYQVLPLLCPTYYTKRGIL"
     gene            2731..3333
                     /locus_tag="VH752_001612"
     CDS             2731..3333
                     /locus_tag="VH752_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289943.1"
                     /translation="MTTKTYFMRSFNLILYLLLIAPICYVFGWLFNLVSVQLSTVTFV
                     NLDTVKNVTDTISSVCYGFALISLLLALLSGLKMIQRLNTDSLGNYCQSIYRTFLLRH
                     FLFQRERVQKVSNFEHQTVTTINPIHNGFNRAVRKCIVDIRKDAITVFLKVPRDQQGQ
                     KILKDTGVQLKEEIVIQHTDYYFSSPVRVRNQLWFIGQKR"
     gene            complement(3713..3994)
                     /locus_tag="VH752_001613"
     CDS             complement(3713..3994)
                     /locus_tag="VH752_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ8289944.1"
                     /translation="MLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFGT
                     LLIAISIYPIMSWLGWWGYGPVAILWLIIMRSALRIERFKKTYDVQTYR"
     gene            complement(4001..4051)
                     /locus_tag="VH752_001614"
                     /pseudo
     CDS             complement(4001..4051)
                     /locus_tag="VH752_001614"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         1348 a          738 c          676 g         1323 t
ORIGIN      
        1 aataatcctg tattctggga cagccgtaga cttacttatt atcctaatgt tattttttgc
       61 caaaagaaaa agcagaaaag acatcattaa catctattta ggacaatttc taggctctgt
      121 tagtctaata ttcctaagtt tgctttttgc atttgtctta aattatattc ctagtaaaga
      181 gattttaggt ttactcggtt tgattccaat tttcctaggc ctaaaagttt tgcttttagg
      241 agattctgat ggagaagcta ttgcaaaaga tggtttgcga aaagacaata aaaacctgat
      301 ttttctagtc gctatgatta cttttgcaag ttgtggcgct gacaatattg gtgtctttgt
      361 cccatatttt accaccttaa atttagcgaa tttgatagtg actttactta cttttctagt
      421 catgatttat ctcttggttt tttctgcaca aaaattagca caagtccctt ctgttggaga
      481 aactttggaa aaatatagca gatggtttat tgccgttgtc tatttaggat tggggatgta
      541 tatcctgatt gaaaacaaca gctttgacat gctatgggct gtgttaggct aggagaaaaa
      601 attatgaaaa aagatagtat ctgtcaagtg gatgttataa atcaacaaaa tgttacaacc
      661 gcaacgaact accttgaaaa ggaaaaagtc caaaaatcac ttcgcatttt atcaaaattt
      721 accgataata aacagataaa tatcatcttt tatctccttg ccgtcgaaga actctgtgtc
      781 tgcgatatag cctgtttatt aaatctcagt atggcatctg cctcccacca tcttcgtaaa
      841 ctagccaatc aaaacatctt ggactctaga agagagggga aaattatata ttattttata
      901 aaagatgagg aaatcagaga tttttttaat caactaggat aacaactatt tttactactt
      961 tctacttttc catgattata gggggattat atatgaaatt ttttgaagaa aattattcac
     1021 aagaaatacc tactcgtatc aaaaatttaa gaaaaaaaca caatattaca caaagcgaac
     1081 taggcaatgc aggtcaagtt agtcaagttg aaagtggtaa gcgaccaatt acgagttcga
     1141 tgttagttta tttaaatgct ctaaccgctt cgagctatac gtatatcgtt tttggagagt
     1201 tagatgagtt tattgagaat ctgtttcatt attttttcag ttctatttta tatagagatt
     1261 tagaggctgt agatgaaaac ctatattctt tcatgagtga tgatttaatt tcaatccaat
     1321 caagttgtct aagtattgcc aagacatttg caaatttcaa tattcaaaga aaaagattta
     1381 tgatttctac tgaaactgaa atggatactt ttcacaagaa agatgacatt gacgtatggg
     1441 ttggtggaaa aagttataac cccgctagga gttttaggaa taatccaata aatgaattaa
     1501 ctgttattga ttttgaagaa atggctgata ttcttttgtt gatgttgaga gataatctaa
     1561 ttaggtcgtt tgaaattaat gtctgcaaca cactgttcga attagataaa aatggtgaac
     1621 cgacaacatt taatctagat aaaattgact caataatcaa taagtggtgg tctgagaatg
     1681 tctcaacaga gataataccc aacctcataa aaaaacttag agaaaatccc ttgtttaata
     1741 tcggatttat ggtaaatgat atattagaac gaatgtataa agaggacata ccaaagtctt
     1801 accttacttc tgttccatta gttatttcca aaaaagcaag gtcaacttta tcgattaagt
     1861 caaacggaag tcaaaaaata gataaatcaa aatttgcaca gattttgtcc ctttagagac
     1921 ggatgactta cacattcaac gtggattatt cttcccttgg agaaagggga tttcagaacg
     1981 cttggcgatt cctgatttag ataaattttc atctaactta caacagggga aaattcctgt
     2041 cataaagcac ttcgaactca atttagataa ggggagtcac tgggctattg ctggaaattc
     2101 aggttctggg aaaccgtacg ctctcactta cttcttgagt gtcctgaaac ctatgtccgg
     2161 cctaattatc attgacccaa aattcgatac ccctagccaa tgggcaagag aaaataaaat
     2221 tgccgttatc cacccagtag aaaaccattc aaaatctgat ttcgtttcac aagtcaatga
     2281 acaattaaac cagtgtgcaa ctctcattca gaaacgacaa gctatcttgt atgataatcc
     2341 caatcatcaa tttacccatc taactattgt tattgatgaa gtcctcgctc tatcagaggg
     2401 agtcaataag aatatcaaag aagctttttt ctccctacta tcacagattg ccttgttggg
     2461 acatgctact aaaatccatc tattcttggg aagccagcgt tttgaccata acactattcc
     2521 catttcagtg agggaacagc taaacgtatt attgcaaatt ggaaatatca atcagaaaac
     2581 cactcaattc ttatttccag acctagatcc agaggggatt gtcattccaa caggacacgg
     2641 aacaggtatc attcaagttg tcgataatga acactcctat caagttctac ccttactctg
     2701 tccaacctac tatactaagc gaggtatcct atgaccacta aaacctactt catgagaagc
     2761 ttcaatctca tattatactt actgcttatt gctccaatct gctatgtgtt cggttggctg
     2821 ttcaatcttg tttcagttca attatctacc gtcacatttg tcaatctgga tacggttaaa
     2881 aacgttactg ataccattag ttctgtatgt tatggattcg ctcttatttc tttacttcta
     2941 gccttattat ctggattaaa gatgatacaa cgcttgaata cagatagcct tgggaactac
     3001 tgccaatcta tttatcgcac ctttctatta agacacttcc ttttccagcg tgaacgagta
     3061 cagaaggtaa gcaactttga acaccagacc gttaccacta tcaatccaat ccataacggg
     3121 tttaaccgag ctgtacgtaa gtgtatcgta gatatacgaa aagatgccat tactgtattt
     3181 ctaaaagttc ctagagacca acaggggcaa aaaatcctaa aggatacggg agtacaactg
     3241 aaagaagaga ttgtcatcca acacacagac tattatttct cttctcctgt tcgagttcgt
     3301 aaccaattgt ggtttattgg tcaaaagcgt taattccttg gggctgtggg tatttattta
     3361 tcttgaaaag attgctgaac taacgcaata actactctac cattcactct accttttatt
     3421 aagcaactat caataaatat tgatacaata aggtttataa cagctaaaat agaaaatact
     3481 taacctttac agaagtaaaa taattgagat gagaaaagcc tatgaaatga acgtttcaaa
     3541 ggcttttttc gtgtttaaaa aaacaaatga aaaaacaaaa tagagagtag ggttattaac
     3601 actgtcaatc ctgtgtacgc aataacaatc catgcttttt gataaggata ttttgctttt
     3661 ctttaatctt atcaatctga tctaagctct caccctttgc tatagcttca agttatcggt
     3721 aagtctgaac atcatatgtc tttttaaatc gctcaattct taaggcgcta cgcatgatta
     3781 tcaaccataa gatagcaacc ggaccatatc cccaccaacc taaccaagac attataggat
     3841 aaatactgat agcaattagg agtgtcccaa ataacataat atgcgtattc ctatcacatt
     3901 gtttaatctc ctcatcctta ataattaaag tcatttttgg gatatcttct gctattagtt
     3961 gctctaaagg aacctgtaag gacttactta acatcaaaag attttgcagg tctggatagc
     4021 ttttaccgtt ttcccagttt gagatagtct tcttgaaaca tacatcattt cagcaagctc
     4081 ttctt
//
LOCUS       DAUGHY010000084         3973 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.084, whole genome shotgun sequence.
ACCESSION   DAUGHY010000084 DAUGHY010000000
VERSION     DAUGHY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.084"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            7..1515
                     /locus_tag="VH752_001615"
     CDS             7..1515
                     /locus_tag="VH752_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ8289945.1"
                     /translation="MLDYYLESDIIDQKILLAILLTEKELTMAAACSQTTLTALKIKQ
                     YLRQFNALFKGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKMLTFLLLDNPKH
                     KSIAVYTRKQGISQSKAYRLIHKLKHYLQDIGLNIVDNTVIGDELKIRYLIALLHKEY
                     GIILYDIQPADIETIHAFIFAAQKNLQPSAFLDRRFLFFDVLLMLTWKRHRYPVHLPH
                     LALFEHLKSLPIFDNIKTIAVDELAPRTRVTFSSDDFDYLFLIYLTTDNSFLSGYWTS
                     HQRQQLYHLITKDPDYHLLIHRLQALVGKYSDIHEHIPNLIPFFKRTLYNLQTLITFD
                     GYYFDQYQGNMLLLDKLETVIKDWLYDTGRQGSISSGHLHLMCLYLEQILESSIAPIN
                     ITVIESQETVGNVIANFITSTIPSYKVELSRVNILSDNIYPYDKPVDLVVTSQKLLPF
                     LKELGVFPKETRLFGLSLDCIQQQREDLIKTILALHQNHYQKRLEELWRIPS"
     gene            complement(1864..3213)
                     /locus_tag="VH752_001616"
     CDS             complement(1864..3213)
                     /locus_tag="VH752_001616"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEQ8289946.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKEEFARILTAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDK
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEALSAELNARL"
     gene            complement(3672..>3973)
                     /gene="speG"
                     /locus_tag="VH752_001617"
     CDS             complement(3672..>3973)
                     /gene="speG"
                     /locus_tag="VH752_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeG"
                     /protein_id="HEQ8289947.1"
                     /translation="ETYLPSEVVRIKKKQFTLQEFDFKIRKFLMEKYNIYDSESRYTS
                     GSLFLATKDSKHYEVDLFNKDDKLLSRDSFFKRYKDNKIFNSEEISHFDIYLKTY"
BASE COUNT         1189 a          777 c          680 g         1327 t
ORIGIN      
        1 attacattgt tagattatta tttggaatcc gatattattg accagaaaat cctactggct
       61 attttgttga cagaaaaaga actgacgatg gcagcagctt gttctcaaac aactttgact
      121 gctttaaaaa taaagcagta tcttcgacaa tttaacgcct tatttaaagg gtacttacgt
      181 attgagctta ttaaatctcg tatttattgt gaggtattaa atgataagcg agaagcattt
      241 ttttatgata tttttgcctt atcagatact ctaaaaatgt tgacattctt attactagat
      301 aatcccaaac acaagtcaat cgctgtttat acacgaaaac aagggatatc ccagtcaaaa
      361 gcttatcgtc ttattcataa attaaaacat tatttacaag acattggttt aaatattgta
      421 gacaatactg ttatcggaga tgaacttaaa atacggtatt tgattgctct tttacataaa
      481 gagtacggca ttatattata tgatatacaa cctgcagaca ttgaaacgat tcatgctttt
      541 atctttgcag cacaaaaaaa cttacaacct tctgcctttt tagatagacg cttcttgttt
      601 tttgatgtgc tgttgatgtt gacttggaag cgccatcgct atcctgttca tttgcctcat
      661 ttagctcttt ttgaacatct caaatccttg cctatttttg acaatatcaa aaccattgcc
      721 gtggatgagc tagcacctcg aactcgtgtc acattttcgt ctgatgattt tgactatctc
      781 tttttaattt atttaacgac tgataactcc ttcttgagtg ggtactggac aagtcatcag
      841 cgacaacaac tttaccattt aatcaccaaa gatcccgatt atcatttact aattcatcgt
      901 cttcaggcac tagttggaaa atattccgat attcatgagc atatccctaa cttaattcct
      961 tttttcaaaa gaactctcta caatttacaa actttaatta cctttgatgg ttattatttt
     1021 gatcaatatc aaggcaacat gctactacta gataagcttg aaaccgttat caaagattgg
     1081 ctttatgaca caggccgtca aggcagtatt agttctggtc acttacactt gatgtgttta
     1141 taccttgagc agatcttgga aagctctatt gctccaatca atattactgt tattgagagc
     1201 caagaaactg ttggtaacgt catagctaat ttcatcacct caactatccc ttcttacaaa
     1261 gttgaactgt cacgggtcaa tatcttatct gataatattt atccctatga taaacctgta
     1321 gatctggttg tgaccagtca aaagctcttg ccttttttaa aagaactcgg tgtttttccc
     1381 aaagagaccc gcttgtttgg tttgagtttg gactgtatcc aacagcagcg cgaagattta
     1441 atcaaaacca tcttagcctt gcaccagaac cactaccaaa aacgtttaga agaactttgg
     1501 agaattcctt cttaattttt tgacagaccc tcctagatac tttcacagtc taggagggtc
     1561 tttaaggttg tcacttgcaa ccttgataag ctctcttttt aaccattgct atttgcctag
     1621 aaacagaact ggaactgttt tagcggttca actcctcttg taggatcttc ttcccttttt
     1681 tgaaggtctt atctgaaatt tggcataata aagtgcgctc ccatgttctg gaagcgcact
     1741 ttattatcgc ttttacttaa ccttgtatcc acttgatgat ggaagtgccg ccccaattct
     1801 tagattgctt ggctaacttt taagctcttg tacaaggaag aagtcttttt gactatctgt
     1861 tgattagagg cgagcattaa gctctgctga taatgcttca aaacctggtt ttccaagaag
     1921 cgcaaacatg ttacgtttgt aagcttcaac acctggttga tcaaatgggt taacagcatt
     1981 catgtagcct gaaaccgcga tagcaagctc aaagaagtaa atagtgtaac caagggtaaa
     2041 ttcgtcttga gctggaagag tgacaaacat gtttggcacg ccgccatctg tgtgggcaag
     2101 aagcacacca tctgtagctt tcttgttaac gaaatcaacg tctttacctt gaaggtaacc
     2161 aagtccatct aggtcttctg ctaattcagg aatgataaca tttttacgag gtttatcaac
     2221 acgaataact gtttcaaaga ggttacggta accttcttgg ataaattgac caagtgagtg
     2281 taagtctgtt gagaagttag ctgaagttgg gtaaatccct ttttggtctt ttccttctga
     2341 ttcaccggct aattgtttcc accattctcc aaagtattga agagatggct cgtagttagc
     2401 caagatttca gtgatgtagc ctttacggta aagcacatta cgaacagcag cgtattggta
     2461 agcaatgttt tcagagattt tgtctgaaga aaggtcttta cgcgctgcat ttgcaccttc
     2521 cataagagct gtgatatctg cacctgatgc agcaattgga agcaaaccaa cagcagtcaa
     2581 gactgagaaa cgaccaccaa cgttgtctgg caccacaaat gtttcccagt tgttagcgtc
     2641 agcttctacc ttaacagcac ctttcacttt gtctgttgta gcgtagatac gtttgttagc
     2701 ttcttcttga ccatattttt taaccaaaag ttctttgaag acacggaaag caatcgctgg
     2761 ctcagtagtt gttcctgatt tagaaatcac gtttactgag aactctttgt cttgaacgta
     2821 ctctacaagg tctgctaagt aagttgatga aatagagttt ccagcgtaaa gaatttgtgg
     2881 ggctttgcgc tctttggcag tttgtaagtt agcaaaatga tgatttaaaa aatcaatcgc
     2941 tgctttggca ccaaggtatg acccaccgat accgatcacc acaagaactt cgctgtctga
     3001 cttaattttt tcagcagcag tcaagatacg tgcaaattcc tctttgtcat agttttctgg
     3061 aagatcaagc caacccaaga aatctgaacc tggtccagtt ccttcacgca ataacttgtc
     3121 agcttctgtg acttggcctt gtaaaaaatc aatttcatgc tgtcccgcaa atgactcgag
     3181 gacttttgaa taatcaaatg taatatgtga catgagtttc gctcccttaa aattttaagt
     3241 ttcattagta tgataacctt ttctatctaa tatttcaatg gtttttagtg gttttcacct
     3301 gtgaaaaagc taggtttttt gaaaaggttt tcgttttttc agacctaaaa catgtgtcaa
     3361 tgcaagcata ttctcatcat agtggttttt taagagaaaa taggatttca tatttcctaa
     3421 aaatatcatg aaaaaaggcc ctgaggccgc tttaccgtaa taacttattt taaggatttt
     3481 atcatttcaa tacgggtaat aaccccatca tgcatatgat caatgcataa agccacacct
     3541 acaaaggcaa ttgaagcccc aatcacctgt aaatactttc tcatgctatc ttcttaacat
     3601 tacttactct cattatctaa aaatggcaat catttgaata cttaaaaagc aaaagggggg
     3661 ggagaatagc actagtacgt ttttaagtag atatcaaaat gactaatttc ttcactatta
     3721 aaaatcttat tatctttata ccttttaaag aaactgtctc gacttaaaag cttatcatcc
     3781 ttattaaata aatcaacttc ataatgttta ctatctttag tagcaaggaa aaggctcccc
     3841 gatgtataac gcgattccga atcatagata ttgtattttt ccattagaaa ttttcttatt
     3901 ttaaaatcaa attcctgtaa agtaaactgt ttctttttta tgcgaacaac ctcagagggc
     3961 aaataagttt ctt
//
LOCUS       DAUGHY010000085         3937 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.085, whole genome shotgun sequence.
ACCESSION   DAUGHY010000085 DAUGHY010000000
VERSION     DAUGHY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.085"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            80..958
                     /gene="mvk"
                     /locus_tag="VH752_001618"
     CDS             80..958
                     /gene="mvk"
                     /locus_tag="VH752_001618"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEQ8289948.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYAALDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLIIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
     gene            940..1884
                     /gene="mvaD"
                     /locus_tag="VH752_001619"
     CDS             940..1884
                     /gene="mvaD"
                     /locus_tag="VH752_001619"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEQ8289949.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSISFLPDTATSDQFYINGVLQNDEEHTKISAIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFNTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDEWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYQAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            1877..2890
                     /locus_tag="VH752_001620"
     CDS             1877..2890
                     /locus_tag="VH752_001620"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEQ8289950.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILTPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFAHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQPALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKELHPAIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQDARDTLISKWQEADIALLYQERWGEN
                     DQP"
     gene            2877..3866
                     /locus_tag="VH752_001621"
     CDS             2877..3866
                     /locus_tag="VH752_001621"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEQ8289951.1"
                     /translation="MTNRKDDHIKYALKYQSLYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVADNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKEVDYLLYGRLQQVN"
BASE COUNT         1205 a          818 c          831 g         1083 t
ORIGIN      
        1 atatcatcgc attttctaat catttagtgt aaaatagtaa ggtaaaagtt tagaagaatt
       61 agtaatgaaa agataaaaaa tgaacgaaaa cattggatat ggtaaggcac acagtaagat
      121 cattttgata ggtgagcatg ctgttgtgta tggctaccca gctattgctt tgcctttaac
      181 agatattgag gtggtttgtc atatttttcc agctgataag ccattggtct ttgattttta
      241 tgatactcta tcaacagcca tttatgctgc cttggattat ttgcagcgac tacaagaacc
      301 aattgcttat gagattgttt cacaagtgcc acaaaagcgt ggtatggggt cttcggcagc
      361 cgtttctatt gcagctatta gagccgtttt ttcttattgt caagaacccc tctcggatga
      421 tttgttggaa attttagtca acaaggcaga aattattgcc cacaccaatc ctagtggttt
      481 agatgccaag acatgcctta gtgaccatgc cattaagttt atccgaaaca ttggctttga
      541 aactatcgaa atcgccttaa atggttatct tatcattgca gatacaggga ttcacggtca
      601 tacacgtgag gccgtcaata aggtagcaca gtttgaggaa acgaatttgc cctatctggc
      661 taaactagga gctttgacac aagcccttga aagagcgatt aaccaaaaaa ataaggtggc
      721 tatcggtcag ctcatgacac aagcgcactc tgctttaaag gccattggcg ttagcatcag
      781 taaagctgac caacttgtgg aggctgccct aagagcaggt gctttaggcg ctaagatgac
      841 aggcggtggc ttgggtggct gtatgattgc cctagcagat accaaagaca tggccgaaaa
      901 aatcagtcac agattaaaag aagaaggagc cgtaaacacg tggatccaaa tgttataacc
      961 gtaacatcct atgctaacat tgctattatc aaatattggg gaaaagagaa tcaagctaag
     1021 atgattcctt cgacctctag catttctttg actttggaaa acatgttcac cacaaccagc
     1081 atttccttct taccagatac tgcaaccagt gatcagtttt acattaacgg tgtcttgcaa
     1141 aatgacgaag aacataccaa aatttctgct atcattgacc aatttcgcca acctggtcag
     1201 gcttttgtaa agatggaaac tcaaaataat atgccaacag ctgcaggttt atcatcaagt
     1261 tccagtggct tatcagcctt ggttaaagcc tgtgaccaac tatttaacac ccagctagat
     1321 cagaaagctt tagctcaaaa ggctaagttc gcctcaggat catcttctcg ttcttttttt
     1381 ggcccagtcg ctgcttggga caaagatagt ggtgctattt acaaggttga gactgacttg
     1441 aaaatggcca tgattatgct ggtcttaaat gctgcgaaaa agccaatttc tagccgagag
     1501 ggcatgaagt tatgccgcga tacctcaact acatttgatg agtgggtaga acaatcggca
     1561 atcgattacc aacatatgct tacctatctc aaaactaata attttgagaa agttggtcag
     1621 ttgacagaag ctaatgcctt ggccatgcat gccacgacaa agacagccaa tcctcctttt
     1681 tcttacctaa caaaagagtc ttaccaggct atggaggctg tgaaagaact gcgtcaagaa
     1741 ggttttgctt gttattttac catggacgca ggtccaaatg tgaaggtcct atgtttagaa
     1801 aaagacttgg ctcaactggc agaacgactt ggcaagaact accgtattat cgtttcaaaa
     1861 acaaaggatt taccagatgt ctaattattg tgtgcaaaca ggtgggaaac tatacctcac
     1921 aggcgaatat gctatcttaa caccaggaca aaaagcctta attcacttta ttccactgat
     1981 gatgacagca gaaattagcc cagcagccca tattcaatta gcttcagata tgtttgccca
     2041 taaagcgggc atgacgcccg atgcctctta tgcactgatt caagcaacgg ttaaaacctt
     2101 tgctgactat ctaggacagt cgattgacca actggagcca ttttccctaa tcataacagg
     2161 aaaaatggag cgcgatggca aaaaatttgg cattggttca agtggtagcg tcaccctctt
     2221 aaccttaaag gccttatcgg cctattatca gatcacttta accccagagt tactctttaa
     2281 actagcggct tataccttgc ttaagcaagg agataatggg tctatggggg atattgcctg
     2341 tatcgcttac cagactttag tcgcctatac ctcctttgac cgagaacagg tcagtaactg
     2401 gctgcagacc atgcccttaa aaaaacttct cgtcaaagat tggggttacc atatccaagt
     2461 cattcaacca gccctgcctt gtgacttttt ggttggctgg actaaaatac ctgctatttc
     2521 aaggcagatg attcaacaag tgacagcgag cattacccca gctttcttaa gaacaagtta
     2581 ccagctaacg caatcagcta tggtagcttt gcaagaaggt cataaggaag aactcaagaa
     2641 aagtttagca ggagcaagtc atctcctaaa agagcttcat ccagctatct accatcctaa
     2701 gctagtaacc ttggtagctg cttgtcagaa gcaagatgct gttgctaaat cttcaggttc
     2761 tggtggtgga gattgtggca tcgcgcttgc ctttaatcag gatgctagag atacccttat
     2821 ttccaaatgg caagaagctg atatcgcatt actttatcaa gaaaggtggg gagagaatga
     2881 ccaaccgtaa agatgatcac atcaaatatg ccctcaagta ccaatcgctt tataatgctt
     2941 ttgatgacat agaactcata catcattcct tacctagcta tgatttgtct gatattgatc
     3001 tcagtactca ttttgccggg caagacttcg actttccctt ttacatcaat gccatgacag
     3061 gaggaagtca aaaaggcaaa gctgtcaatg aaaaattggc caaagtagca gcagcaacag
     3121 ggattgtcat ggtgacaggg tcttatagcg ctgctttaaa aaatcctaac gacgattcct
     3181 atcgtttaca tgaggtggca gataacttga aactagccac gaatattggt ctagataaac
     3241 ctgtggcgct aggacaacaa acggttcaag aaatgcagcc cctcttttta caggttcatg
     3301 tgaatgtgat gcaagagttg ttgatgccag agggtgagcg cgtctttcat acctggaaaa
     3361 aacacctcgc tgaatacgct agtcaaatac cagttcctgt cattctcaaa gaagttggtt
     3421 ttggcatgga tgtcaatagt atcaagctag cacatgacct aggcattcaa acctttgata
     3481 tttcaggaag aggagggacc tcatttgcct atattgaaaa tcaaagagga ggagaccgct
     3541 cttacttaaa cgattgggga caaaccaccg ttcagtgctt gctaaatgca caaggactga
     3601 tggaccaagt ggaaatctta gcttcgggtg gtgtcagaca ccccttggac atgattaaat
     3661 gtttcgtcct aggagcacgt gcagtgggac tctcacgcac cgttttagaa ttggtcgaaa
     3721 aatacccaac cgagcgtgtg attgctatcg ttaacggctg gaaagaagaa ttaaaaatca
     3781 ttatgtgtgc tcttgactgt aaaactatta aagaattaaa ggaagtcgac tacttactat
     3841 atggacgctt gcagcaggtc aattaggctt catcacctct acaatagtgc cacctactac
     3901 aaagatgata gagctttttt agaacataat ataaaat
//
LOCUS       DAUGHY010000086         3926 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.086, whole genome shotgun sequence.
ACCESSION   DAUGHY010000086 DAUGHY010000000
VERSION     DAUGHY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.086"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            52..183
                     /locus_tag="VH752_001622"
     CDS             52..183
                     /locus_tag="VH752_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exfoliative toxin"
                     /protein_id="HEQ8289952.1"
                     /translation="MEKEAKQMIDLKRNLFKIDVRAQKDEEKVFMRTAWFLVGLNPL"
     gene            233..1012
                     /locus_tag="VH752_001623"
     CDS             233..1012
                     /locus_tag="VH752_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ8289953.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDGFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRHEFA
                     KLSIPDVSEGRAYDVTGVEQIGPFDITFLKTVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQAGVKKLVLTHMPPVP
                     PEGIDPDNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
     gene            1012..1527
                     /locus_tag="VH752_001624"
     CDS             1012..1527
                     /locus_tag="VH752_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEQ8289954.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGADSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(2141..3373)
                     /locus_tag="VH752_001625"
     CDS             complement(2141..3373)
                     /locus_tag="VH752_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HEQ8289955.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNTSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPVSKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNVYQDLQAIGNDIVANTPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQSQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDYLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDNDAILASLENQLLNRQEPLSLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            3573..3671
                     /gene="ffs"
                     /locus_tag="VH752_001626"
     ncRNA           3573..3671
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VH752_001626"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3773..>3926
                     /locus_tag="VH752_001627"
     CDS             3773..>3926
                     /locus_tag="VH752_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEQ8289956.1"
                     /translation="MKTNILTIIILSCVFSYGSQLAYADENLKDLKRSLRFAYNITPC
                     DYENVEI"
BASE COUNT         1213 a          660 c          843 g         1210 t
ORIGIN      
        1 gcaaacaacg gtatatttct gtccatttgg aagattattt agtccaatta tttggagaaa
       61 gaggctaagc agatgattga tttaaaaagg aatcttttca aaattgatgt ccgggcacaa
      121 aaggatgaag agaaagtttt catgcgtacg gcgtggtttt tggtgggtct caatcctctt
      181 tgacactaat ttttgttata ataagagtta aagcaagaaa gaagggtttg agatgaaatt
      241 aaccacctta gggtcttggg gagcctatcc ctatcaagat gcaggcacaa cctcttattt
      301 ggtcacagga catgacggct ttcagttatt gatggatgct ggtagtcgag cgctcaatga
      361 gctggaaaaa gaaatcagtc ctctcgattt ggatgcagtg attattagcc attaccatcc
      421 agaccacgtg gccgatttag gcgtgttgcg ccattatttt caactttatc ctaaacacct
      481 gtggcagcct aaagtattgc ccatttatgg gcatgatgag gaccgacacg aatttgctaa
      541 gttaagcatt cctgatgttt cagaaggtag agcctatgat gtcactggag ttgaacagat
      601 tggtcctttt gacattacct ttcttaaaac agttcatcca gttgtgtgtt atgcctttcg
      661 aattgtagaa agagcgactg gtcaggtact tgtctttact ggagacagtg gttattttga
      721 aggattggca gacttcgcaa aagaagcaga ccttttctta gcagatgttt acttatacga
      781 aggcaatgaa aatcatatgg cccatttgac cagtaaggaa gcaggactaa tggctagcca
      841 ggcaggtgtt aagaagcttg tcttgaccca catgccaccg gttcctccag aaggtattga
      901 tcctgacaat cacttggaag tgttgaggca agaaacacaa gcctatgcag gtaatattcc
      961 tgttgacctt gccttaccac acaaatcttg gaacttgggg agtctttctt gatgccatat
     1021 agtttagaag agcaaactta tttcatgcaa gaggctttaa aagaagccga aaaatcctta
     1081 cagaaagctg aaatccccat tggctgtgtc attgtcaagg atggtgagat tattggtcgt
     1141 gggcacaatg cgcgtgaaga gagtaaccaa gccattatgc acgctgaaat gatggctatt
     1201 aacgaggcta acgctcatga aggcaactgg cgtttattag ataccacact ctttgtgacc
     1261 atcgagcctt gtgtgatgtg tagtggtgcc attgggctag caaggattcc gcatgttatt
     1321 tatggagcca gcaatcaaaa atttggtgga gcagacagcc tttaccagat tttgacagat
     1381 gagcgcttaa accatcgcgt gcaagttgaa cgaggcctgc tagcagcaga ctgtgctaac
     1441 atcatgcaaa cctttttccg ccaagggcga gagcgcaaaa aaatagctaa gcatttgatc
     1501 aaggaacagt cagatccctt tgactagagt tttaaggagt ttgttataac atgcattttt
     1561 ggtgaagggt agcctgtcaa aatactgagt gaaacgttag accatttttc caatggttgt
     1621 tagattcaac atacgaagat agaatataca aaagggcgtt tccaagtact agaagcgctc
     1681 tttttcagac ccaatcgaga tgaaaacaat cgaaaaataa aacacgtgat gactagatga
     1741 gttggtcatt cttaaaaaga tttactatct aaaaacagat agtaatggct caccttattt
     1801 ttgtttttag taaacttatc aagctcagac ttctagggct cagagctgtc agttatctgt
     1861 ggcgtagatt gatactgaca cagggattaa aaacaagtga cttataggca tggcttgaat
     1921 tatttaatct tttgtcttag ctgatatatt ttgctaaagc attaaaaaag agctcatcac
     1981 tgttgctaat gttacaagca ttagatgtgc tcaatctgaa aaggttatat ctattgaaga
     2041 cggttagaat aataatcgcc ttcaattgtg ttagtatatc aatttcttgt aatgtttgac
     2101 aaagataatc atcttaactt tgtgtttcat gtgtctaaac ctagttccta ctagcaaatg
     2161 aaatactaag agtctctggt aagagtccat aggtaaagcc cgtataatta ttccaatact
     2221 gattgaaaag gttatgataa cctgtttgat tcgtcgttaa atcagttacc atttgttgat
     2281 aatcatcttg atgagcaaat tgtagagaca gtggctcttg tctatttaac aactgatttt
     2341 caaggcttgc aaggatggcg tcattgtcaa acgttgagat atagctttga tgacgtttag
     2401 aataaagcaa agaatcatct gtacaagaag gtatagtcca aacattttca aaacgttctt
     2461 tagtattaaa agcaatgtct ttgcttggct gatgtgataa ggccattaga taatctggta
     2521 ggcataaaaa actgtaattg atagttcctt gcttttgatg tgagagatgg ttatcaaaaa
     2581 cagggtcacc ccaagtgaca tcaacgccat aataagtgtt attaatttga acagcattcc
     2641 atgcatgtgc aaaagattgc tgaggttgct gagatgtacc agttccacgg atataggcga
     2701 ctggaatgcc agctttttga caaagaaact gaaaggcttg ggcatagcca ttacaaacag
     2761 ataaatgatc aataaaaaca cttttaatgt cttgattaga agcgacctgg gattgactgc
     2821 ctgattgata ggcttcaaag gcttttttat tgtagtctgt gtctcgaatg atgacctcat
     2881 aaaaatattt gacctgctca tagcgatcct ttgatggcgt gtttgcgacg atatcattac
     2941 cgattgcttg caagtcttga taaacgttct tggcatcttc tgggatagga aaggtgacaa
     3001 agaccgtttg atcagaaaac tcaaatcgat aatcggcaga tgtaatccag taaaattcgg
     3061 gattatccat cacaaaggcg tcaatcgttt taattaagct ttttttagaa accggcgtca
     3121 aactgatgat ttcacgaaac tgtgctagac tatcgtataa agtgaggtaa ttttcttgtt
     3181 cggttttgga taattgttgg aaataatacc gttgtcttac ttgtttgacg atttgttggc
     3241 gcttttttcc ggttaaagaa aaaacagaat cggtattaga aggtttgcta gtgttgtgac
     3301 acgcagacat accaactgtc ataaggccaa ttactgttaa gattttaatc ttttgtttca
     3361 tcataactcc cattctaatc taataatctt attataacat agagaatagc tggaatgggg
     3421 agagggaaag attttcaaaa agcaaaatcg tgtttggaaa gggtcatgat ttttctaggt
     3481 attcagtcca tcttgtccca aaatgaagct atgctcactt gaaagtgatt ttacttagtt
     3541 ggaaaacttg caaactgtcc gtaatatgat ataataatag gcggagcaac acttgtgcgt
     3601 gaagtgggtc aggggaggaa tccagcagcc ctaagcgatt ttcgggtgtg tgctcttttt
     3661 ttgttgactt tactaaagta atctcttcct tctatagtgt catcagttgt tgaaaccgat
     3721 tgatattttt atttaggtta agaggtataa aacaggacat aaaaagtttt aaatgaaaac
     3781 aaacattttg acaattatca tattatcatg tgtttttagc tatggaagtc aattagctta
     3841 tgcagatgaa aatttaaaag atttaaaaag aagtttaagg tttgcctata atattacccc
     3901 atgcgattat gaaaatgtag aaattg
//
LOCUS       DAUGHY010000087         3895 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.087, whole genome shotgun sequence.
ACCESSION   DAUGHY010000087 DAUGHY010000000
VERSION     DAUGHY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.087"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(154..3657)
                     /gene="scpA/B"
                     /locus_tag="VH752_001628"
     CDS             complement(154..3657)
                     /gene="scpA/B"
                     /locus_tag="VH752_001628"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEQ8289957.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPVTEQA
                     VETPQPTAVSEEVPSSKETKTPQTPDDAEETIADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEGA
                     MPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETK
                     KAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSPD
                     KQLTETAMVKTDDQQDKEMPVLSTNRFEPNKAYDYAYANRGMKEDDFKDVKGKIALIE
                     RGDIDFKDKVANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDNP
                     QKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKL
                     SGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFS
                     PRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYY
                     QATVQTDKVDGKHFALAPKVLYEASWQKITIPANSSKQVTVPIDASRFSKDLLAQMKN
                     GYFLEGFVRFKQDPTKEELMSIPYIGFRGDFGNLSAVEKPIYDSKDGSSYYHEANSDA
                     KDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTF
                     AKQDDDSHYYIHRHANGEPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNV
                     VWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISS
                     GAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDED
                     LPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEG
                     HSNKPEQDGSGQTPDKKPEAKPEQDGSDQAPDKKPEAKPEQDGSGQTPDKKPETKPEK
                     DSSGQTPGKTPQKGQPSRTLEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMT
                     TFFFGLVAHIFKTKRQKETKK"
BASE COUNT          965 a          786 c          811 g         1333 t
ORIGIN      
        1 atcttttatc acagcaactg aaaagataag caaaaggcaa ttgttgacag ctatttgtcc
       61 taacttcttt ttgatgaggc agcacatcta aaaaaataga gagaaccaaa ttggttctct
      121 ctattaaata agctcttatc agtttaagta atactatttt ttagtttctt tttggcgttt
      181 tgttttaaag atatgagcta ctaatccaaa gaagaaagtg gtcataacta acttaaggag
      241 atgtaaacga tttgtatcct tgtcattagt cgttggtaac tgatctcttg ttgatgcttt
      301 tgtagctaaa gcacgcttag aagagcgttt ctctagagta cgagaaggtt gacctttttg
      361 aggagtttta cctggtgttt gacctgaact atctttttct ggtttagttt ctggtttttt
      421 atctggtgtt tgacctgaac cgtcttgttc tggtttagct tctggttttt tgtctggtgc
      481 ttgatctgaa ccgtcttgtt ctggtttagc ttctggtttt ttatctggtg tttgacctga
      541 accgtcttgt tctggcttat tagagtggcc ctccaatagc ttagtcactg gtgtataagt
      601 gatattacca gccatatctt caacaacata agtaaagtct gacattttca atggaacagt
      661 agcgccttcc attgtttcag cctcttcagg aagagtaaag gtaccatctt catttggaga
      721 aatatactct gttgttggca gatcctcatc catataagtg taagcaatac gctcacggta
      781 aaccggttgg ctggtttttg gtttagatgc aagtgtcaaa cgacgatctt ctgttgagaa
      841 tgttgccgat gttgcgactt caggtgtcgt attgtctaca atcacatcaa aatcagtgtg
      901 ttgttctttt gcacctgaac taatcggagt gtagcggaca cgataggtgt aggttccgtt
      961 agcaacaact ttgccgtctt tatctttacc gtcccaacga gttttttcaa aacgggttga
     1021 accaagtgtg cttgccaagt cattgttgta gtttttaaca acttgctcgg ttacctcact
     1081 tgtccaaaca acatttcctt ctttgtccaa gacttcagcc acaaggtttt tagcattacg
     1141 caagaaagta ccttggaatt ggacataatc tctgttaccg tccccatttg gagagatcgc
     1201 agcatatggc tcgccattag cgtgacggtg gatatagtag tggctatcat cgtcttgttt
     1261 tgcaaaagta cctgcaaaaa tggtttctgt gatctctgaa gattcgatat cctctatgtt
     1321 ttcaacccct tctttgacag ccttaataat cgtccatggg ttagactctg tggtaagtgc
     1381 tgtaaagttg tttttcagag cgtaaaactg taatccatca ccatctaatt ggtctttggc
     1441 atcactattt gcttcatgat agtagctgct accgtctttg ctatcataga ttggtttttc
     1501 tacggctgac agattgccaa aatcacctcg gaaaccaata tatggaatgc tcataagctc
     1561 ttcttttgta ggatcttgtt tgaaacgaac aaaaccttct aagaaatagc catttttcat
     1621 ttgggcaagc aagtccttgc taaatcgact agcatcgatt ggaacggtga cttgtttgct
     1681 gctattggct ggaattgtga ttttttgcca tgatgcctca tacaatactt taggagccaa
     1741 ggcaaagtgt tttccatcta ctttatctgt ttgaacagtt gcttggtaat acaactcttg
     1801 aggtttatca gatttgttgt gaacagttac tgttacttca aatttatcag aaacattgtt
     1861 caggtgaacc ttgcttgagg tattatcctt atctgtcaca tacatcgttg ctgctgaagc
     1921 ttttttagca tcgaccgctc ctgctccttg ttggcgagga gaaaaataag ctttttcatc
     1981 ttcatcatat aaggcagttg ctgagctcat caatactttt ttagctaaat caagacgctc
     2041 tgatggtgtc atatcaggat actgtgtctc atattgcttt tgcaacagtc ccatgatacc
     2101 cgctactaat ggcgcagaca tactagttcc agaaagtttg gcatacttgt tgttagccac
     2161 tgatgacaaa atatcttggc cgggtgctgc aatatctggc ttaatattgc cgtcagctgt
     2221 cagaccccag cttgagaagc ggcttagttt ggtgccactt gctgttggca ataccttagg
     2281 tgtcgcattg aaggtgatgg ttttttgggg attgtctttt aataagagac cgtcttttcg
     2341 actgataaag gccgcaggca tctgatcaac atttggcaat tcaatcggga agcccttgtc
     2401 ctgattgtca tagatcaaga cccctacagc accagctttt ttagcgtttg caaccttatc
     2461 tttgaaatca atatcgccac gttcaataag ggcaatcttg cctttgacat ccttaaaatc
     2521 atcctctttc atcccacgat tagcataagc atagtcgtaa gccttgtttg gctcaaaacg
     2581 gtttgttgaa agaacaggca tttctttatc ttgctgatcg tctgttttga ccatagcagt
     2641 ttcagtgagc tgtttatctg ggctgtaaga agcaactgtc aatgttgagt ctgccgctgc
     2701 aggtgtccca accaccccat aatcaggatg atctgctaga ggtagacggg tcttgccccc
     2761 aaagctacta tcattaccag ctgaggtcac aatgctaaca ccttttgatt tggcatagtc
     2821 aaaggctttt ttggtttcgt ctgggaggtt agcgtaggct agtgcagcat taccaaagct
     2881 catattaatc accttagctc ccaagttgac agcatctctg atagcttgag cgtagttacg
     2941 agcatagtct gctagtccat ttacaatttc gacacgcatc aaaagcaatt gagcctcagg
     3001 catcgcacct tctaggcggt aaggttcttt cgtttcagat ggagcatttc ctgacaagat
     3061 ccctgacacg tgtgtgccgt gctcttgatc gacagcggtt ttaccatctt tgctatagtc
     3121 gtggtaataa gcaaccttat cattgaccca ctcgccatag gtaataccgt gctctttttt
     3181 agctttttca agatcttctt ttgattggta acgtgctttg gttttgtctg ttaagcgcca
     3241 cgcttcatga tttttatcaa aaccagcatc aatcactgca acaacagtcc cagctccctt
     3301 gcctgctttt tcctgcaggg ttttgacctg agaagggtcg ttcaaatccc taatagtcgc
     3361 ttttgaggtt gctggtgtat cagcagtttt agcaggagct tgaggggcta gatcattagc
     3421 gtcatctgct attgtttctt ctgcgtcatc aggagtttgt ggggttttgg tttcctttga
     3481 tgatggtact tcctcagaaa ccgctgttgg ttgtggggtt tctacggctt gttcggtaac
     3541 aggagtgtct tctgtcacag tatttgcttt aatgtctgat tgtgcattga gcaagatgct
     3601 cgtagacatg agcgcaatgg caagtttatc aaatggtaat ttttgttttt tacgcaatgt
     3661 gtcgtccttt cctgatgaga gactttgtct ttatgaccat taagaaaaga gagaatcaaa
     3721 cgattctctc ctagctgtcc atctgtgcgt ggagaaataa ggataatatc ttttttaaag
     3781 agcaaacagg ggttatttgc atatgataca gctatttttc ttgcttttgc cataatgatt
     3841 aaatgtatct catttatcaa ttatattact aacatcttta ttataaaaaa aagac
//
LOCUS       DAUGHY010000088         3827 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.088, whole genome shotgun sequence.
ACCESSION   DAUGHY010000088 DAUGHY010000000
VERSION     DAUGHY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.088"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            215..1846
                     /gene="mga"
                     /locus_tag="VH752_001629"
     CDS             215..1846
                     /gene="mga"
                     /locus_tag="VH752_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Mga"
                     /protein_id="HEQ8289958.1"
                     /translation="MYVSKLFTSQQWRELKLISYVTENADAIGVKDKELSKALNISML
                     TLQTCLTNMQFMKEVGGITYKNGYITIWYHQHCGLQEVYQKALRHSQSFKLLETLFFR
                     DFNSLEELAEELFVSLSTLKRLIKKTNAYLTHTFGITILTSPVQVSGDEHQIRLFYLK
                     YFSEAYKISEWPFGEMLNLKNCERLLSLMIKEVDVRVNFTLFQHLKILSSVNLIRYYK
                     GYSAVYDNKKTSHRFSQLIQSSLEIQDLSRLFYLKFGLYLDETTIAEMFSNHVNDQLE
                     IGYAFDSIKQDSPTGCRKVTNWVHLLDELEIRLNLSVTNKYEVAVILHNTTVLKEEDI
                     TANYLFFDYKKSYLNFYKQEHPHLYKACVAGVEKLMRSEKEPISKELTNQLIYAFFIT
                     WENSFLKVNQKNEKIRLLVIERSFNSVGNFLKKYIGEFFSITNFNELDALTIDLEEIE
                     KQYDVIVTDVMVGKSDELEIFFFYKMIPEAIIDKLNAFLNISFADSLPLDKPIKNPLD
                     FHRKEVILPTPPNKLHAPLHNLDSLTAATQKQIHH"
     gene            1990..3717
                     /gene="emm"
                     /locus_tag="VH752_001630"
     CDS             1990..3717
                     /gene="emm"
                     /locus_tag="VH752_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein"
                     /protein_id="HEQ8289959.1"
                     /translation="MAKNTTNRHYSLRKLKTGTASVAVALTVVGAGLVAGQTVRADHS
                     DLVAEKQRLEDLGQKFERLKQRSELYLQQYYDNKSNGYKGDWYVQQLKMLNRDLEQAY
                     NELSGEAHKDALGKLGIDNADLKAKITELEKSVEEKNDVLSQIKKELEEAEKDIQFGR
                     EVHAADLLRHKQEIAEKENVISKLNGELQPLKQKVDETDRNLQQEKQKVLSLEQQLAV
                     TKENAKKDFELAALGHQLADKEYNAKIAELESKLADAKKDFELAALGHQHAHNEYQAK
                     LAEKDGQIKQLEEQKQILDASRKGTARDLEAVRQAKKATEAELNNLKAELAKVTEQKQ
                     ILDASRKGTARDLEAVRKAKAQVEAALKQLEEQNKISEASRKGLRRDLDASREAKKQV
                     EKDLANLTAELDKVKEEKQISDASRQGLRRDLDASREAKKQVEKALEEANSKLAALEK
                     LNKELEESKKLTEKEKAELQAKLEAEAKALKEQLAKQAEELAKLRAGKASDSQTPDAK
                     PGNKAVPGKGQAPQAGTKPNQNKAPMKETKRQLPSTGEAANPFFTAAALTVMATAGVA
                     AVVKRKEEN"
BASE COUNT         1454 a          711 c          724 g          938 t
ORIGIN      
        1 tccaattttt gggtcatact ttataacatt tttataaagt atgacccaaa aagaaataaa
       61 tgcgtgatcg tgactgtttt ttggatttta atgaaagaat ttgaaaggca ttttatggta
      121 tactttttct tgatataggt cgtgctgact taatgaaata cctcacgaaa taacaagaaa
      181 atcaagaaaa ttaaaaagaa gggtatacaa ggtaatgtat gtaagtaagt tgtttacaag
      241 tcaacagtgg agagaactaa aattaatctc atacgtaaca gaaaacgctg acgccattgg
      301 cgtcaaagat aaagaactca gcaaagctct caacatctcc atgttaacat tgcaaacatg
      361 tctcactaac atgcaattta tgaaggaagt gggaggaatt acctataaaa atggttatat
      421 tacaatctgg taccatcaac attgcggttt gcaagaagtc tatcagaaag ctctgagaca
      481 ctctcaatca tttaaattgc tggagaccct ttttttcaga gacttcaact ctttggaaga
      541 attagctgaa gagctctttg tgagcctttc aacactcaag cgtctcatca aaaaaaccaa
      601 tgcctatttg acgcatacct ttggtattac tattttaact agtcccgtac aagtatcagg
      661 agatgaacac cagattcgac tattttacct caagtatttt tcggaagcct ataaaatctc
      721 agagtggcct tttggggaaa tgctaaactt aaagaactgt gagcggctac tcagtttaat
      781 gataaaagag gtcgatgtga gggttaattt tacgttattt cagcacctaa aaatactaag
      841 ctcagttaac ttgattcgct attacaaggg atactctgcc gtctacgaca acaaaaaaac
      901 cagtcaccgt ttttctcagc tcatccaatc atccttagaa atccaagacc tttcacgctt
      961 attttacctc aaatttggac tatacctaga tgagactacc atagctgaga tgttctctaa
     1021 tcacgttaat gaccaattag aaatcggtta tgcgtttgat agcatcaaac aagactcacc
     1081 aacgggctgt cgaaaagtga ccaactgggt tcatctcctt gatgagttag aaatcaggct
     1141 gaatctcagc gtcaccaaca aatacgaagt agctgtcatc cttcataaca ctaccgtctt
     1201 gaaagaagaa gatatcaccg ctaattacct gttcttcgat tacaaaaaaa gttacctcaa
     1261 cttttacaag caagaacacc ctcatcttta taaagcatgt gtagcaggtg tagaaaaact
     1321 gatgcgttca gagaaagaac ctatcagcaa agagttgact aaccagttga tctacgcctt
     1381 tttcatcact tgggaaaata gtttcctaaa agtaaatcaa aaaaatgaaa aaattcgtct
     1441 tctggtgatt gaaagaagtt ttaacagtgt tggtaatttc ctaaaaaagt acatcggaga
     1501 gttttttagc atcacaaact tcaatgagct agatgctctg actatcgatc tagaagagat
     1561 tgaaaaacag tacgatgtga tcgtgacaga tgttatggta ggaaaaagcg atgagctaga
     1621 aattttcttt ttctacaaaa tgattccaga agcgattatt gataagctca atgcgttttt
     1681 aaacatcagc tttgcagaca gcttgccact agacaagccc atcaagaacc ccttggactt
     1741 tcatcgcaaa gaggttatct tacccactcc ccccaacaag ttgcatgccc ccctccacaa
     1801 tttagacagc ctaaccgcag caactcaaaa acagattcat cattaatagc atttaggtca
     1861 aaaaggtggc aaaagctaaa aaagctggtc tttacctttt ggcttatatt atttacaata
     1921 gaattattag agttaaaccc tgaaaatgag ggttttttcc taaaaaatga taacataagg
     1981 agcataaaca tggctaaaaa taccacgaat agacactatt cgcttagaaa attaaaaaca
     2041 ggaacggctt cagtagcggt tgctttaaca gtcgtaggag cagggttagt agcagggcag
     2101 acagtaagag cagatcatag tgatttagtc gcagaaaaac aacgtttaga agatttagga
     2161 caaaaatttg aaagactgaa acagcgttca gaactctacc ttcagcaata ctatgataat
     2221 aaatcaaatg gatataaagg tgactggtat gtacaacagt taaaaatgtt aaatcgtgac
     2281 ttggaacaag cgtataatga gcttagcgga gaagcacata aagatgcctt agggaaactg
     2341 ggaattgata acgctgacct aaaagctaaa attactgaac tggaaaaatc tgttgaagag
     2401 aaaaatgatg ttttatctca aattaaaaag gaactcgaag aagcagaaaa agatatacaa
     2461 tttggacgtg aagtgcacgc agctgatctt ttaaggcata aacaagaaat tgctgaaaaa
     2521 gaaaacgtta tatctaagct caatggggag ctgcaaccac ttaaacaaaa agtggatgag
     2581 acggatcgta atctgcaaca agaaaaacaa aaagttttaa gtttagagca acagctagct
     2641 gtcactaaag aaaatgctaa gaaagatttc gaattggctg cattaggcca tcaacttgca
     2701 gacaaagaat ataatgctaa aattgctgaa cttgagtcaa aattggcaga tgctaagaaa
     2761 gattttgaac tagcagcatt aggtcaccaa catgctcata atgagtatca agcaaaacta
     2821 gcagaaaaag atggacaaat caaacaacta gaagagcaaa aacaaatcct agatgctagc
     2881 cgtaaaggta cagcacgaga ccttgaagct gttcgccaag ctaaaaaagc tacggaagct
     2941 gaattaaaca acctcaaagc agagcttgca aaagttacag aacaaaaaca aatcttagat
     3001 gctagccgta aaggtacagc acgagatctt gaagcagttc gcaaagcaaa agcacaagtt
     3061 gaagctgctc tcaaacaact tgaagaacaa aacaaaattt cagaagcaag ccgcaaaggt
     3121 cttcgtcgtg acttggacgc atcacgtgaa gctaagaaac aggttgaaaa agatttagca
     3181 aacttgactg ctgaacttga taaggttaaa gaagaaaaac aaatctcaga cgcaagccgt
     3241 caaggccttc gtcgtgactt ggatgcatca cgtgaagcta aaaaacaagt tgaaaaagct
     3301 ttagaagaag caaacagcaa attagctgct cttgaaaaac ttaacaaaga gcttgaagaa
     3361 agcaagaaat taacagaaaa agaaaaagct gagctacaag caaaacttga agcagaagca
     3421 aaagccctca aagaacaatt agcaaaacaa gctgaagaac ttgcaaaact aagagctgga
     3481 aaagcatcag actcacaaac ccctgatgca aaaccaggaa acaaagctgt tccaggtaaa
     3541 ggtcaagcac cacaagcagg tacaaaacct aaccaaaaca aagcaccaat gaaggaaact
     3601 aagagacagt taccatcaac aggtgaagca gctaacccat tcttcacagc ggcagccctt
     3661 actgttatgg caacagctgg agtagcagca gttgtaaaac gcaaagaaga aaactaagct
     3721 atcattttgt aatactgagt gaacatcaag agagaaccag tcggttctct cttttatgta
     3781 tagaagaatg aggttaagga gaggtcacaa actaaacaac tcttaaa
//
LOCUS       DAUGHY010000089         3775 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.089, whole genome shotgun sequence.
ACCESSION   DAUGHY010000089 DAUGHY010000000
VERSION     DAUGHY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.089"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..328
                     /locus_tag="VH752_001631"
     CDS             <1..328
                     /locus_tag="VH752_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEQ8289960.1"
                     /translation="NIDITDLIVIYDDLDMEVSKLRLRSKGSAGGHNGIKSIIAHIGT
                     QEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAISLTLDRVVNAVKFYLQENDFEKT
                     MQKFNG"
     gene            331..>3775
                     /gene="mfd"
                     /locus_tag="VH752_001632"
     CDS             331..>3775
                     /gene="mfd"
                     /locus_tag="VH752_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEQ8289961.1"
                     /translation="MNILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKALAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLRNPKSQGVLIVSLSGLRTLLPNPDVFTKSQIQLTVGEDYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GIFINPASDLIFEASDFQRGIEQLEKALQTAQDDKKSYLEDVLAVSKNGFKHKDIRKF
                     QSLFYEKEWSLLDYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSNLN
                     YFTDNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIKRY
                     QKNQTTVIVQVESQYAYKRLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFYFA
                     DEKLALITEREIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLGIE
                     TIQIQGIHRDYVTIQYQNSDRISLPIDQIGSLSKYVSADGKEPKINKLNDGRFQKTKQ
                     KVARQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKEIK
                     ADMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENFKA
                     RFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIVID
                     EEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIASKQGKTTVRQKGNTEINLQIDAYLPDDYISDERQK
                     IDIYKRIREIQSREDYLNLQDELIDRFGEYPDQVAYLLEIALLKHYMDNAFAELVERK
                     NNQVIVRFEVTSLTYFLTQDYFEALSKTHLKAKISEHQGKIDIVFDVRHQKDYRILEE
                     LM"
BASE COUNT         1253 a          588 c          747 g         1187 t
ORIGIN      
        1 taatattgac ataacagatt taattgttat ctatgatgat ttagacatgg aagtcagtaa
       61 attacgttta cgtagtaagg gttcagcagg aggacataat ggcattaagt caatcattgc
      121 ccacattgga actcaggaat ttaaccgaat caaagttggt attggacgac ctttaaaagg
      181 tatgactgtt attaaccatg tgatgggcca attcaatacc gaagataata ttgctatttc
      241 gttaactctt gacagagttg tcaatgctgt caagttttat ttacaagaaa atgattttga
      301 aaaaacaatg cagaaattta atggataatc atgaatattt tagaattatt tagtcagaat
      361 aagaaagtcc aatcctggca ctctggatta accaccttag gaagacaact ggtaatgggg
      421 ttatcgggtt caagtaaagc attggctata gcttccgctt atttagatga tcaaaaaaaa
      481 atagttgtgg ttacatcaac tcaaaatgag gttgaaaaat tagccagcga tttatctagt
      541 ttacttgatg aagaacttgt tttccaattt tttgcagacg atgtggctgc agcggaattt
      601 atctttgcgt caatggataa agctctatca agaatagaaa ccctgcaatt tttaaggaat
      661 cctaaatctc agggcgtttt aattgttagt ttatcaggct taagaacttt attgccaaac
      721 ccagatgttt ttacaaagag tcagattcaa ctaacagttg gagaagatta tgatagtgat
      781 actcttacta aacaactgat gacaattgga tatcagaagg tctcacaggt tattagtccg
      841 ggagaattta gccgtcgagg ggatatttta gatatctatg agattacaca agaattgcct
      901 tatcgattgg aattttttgg tgatgatatt gatagtatta ggcaatttca tccagaaact
      961 caaaaatctt ttgaacaact agaaggtatt tttattaatc cagcaagtga tcttattttt
     1021 gaggctagtg attttcaacg tggcattgag caattagaaa aggctctaca aacagcacaa
     1081 gatgataaaa aatcttattt agaagatgta ttagctgttt caaaaaacgg ttttaaacat
     1141 aaggatatcc gtaaatttca atcattattt tatgaaaaag agtggtcatt attagattat
     1201 attcctaagg gaacgccaat cttttttgat gattttcaaa aactagttga taaaaatgca
     1261 agatttgatt tagagattgc taatctcttg acagaagatt tacagcaagg aaaggctctt
     1321 tccaatctta actactttac agataattat cgagagctta ggcactataa gccagcgacc
     1381 ttcttttcaa attttcataa gggacttgga aatatcaaat ttgatcagat gcatcagcta
     1441 actcagtatg ccatgcagga attttttaat caatttcctt tgttgattga tgagattaaa
     1501 cggtatcaaa aaaatcaaac aacggttatt gtacaggtag agtctcagta tgcttacaaa
     1561 cgacttgaaa aatcttttca agattaccaa tttcgccttc ctttagtgag tgctaatcaa
     1621 attgtttcac gtgaatcaca aattgtaatt ggagctatct ccagtggttt ttattttgct
     1681 gatgaaaagt tagcgttaat cacagagcgt gaaatttatc ataaaaagat caaacgacgc
     1741 gctagacgat ctaatattag caatgctgag cgtttgaaag attacaatga gttagcagta
     1801 ggtgattacg tggttcataa tgtccatggt atcggtcgct ttcttggaat tgaaacgatt
     1861 cagattcagg gaatccatcg agattatgtt actattcaat atcaaaattc agaccgtatt
     1921 tctcttccaa ttgaccaaat tggtagctta tcaaagtacg tttctgctga tggaaaagaa
     1981 cctaaaatta ataaactcaa tgatggtcgt ttccaaaaga caaagcaaaa ggtcgctaga
     2041 caagtagagg atattgctga tgaccttcta aaattatatg ctgaaagaag tcagcaaaaa
     2101 ggattttcat tttcaccaga tgatgacttg cagcgcgctt ttgatgatga ttttgctttt
     2161 gtagaaacag aagatcaact taggtctata aaggaaatta aagctgatat ggagagcatg
     2221 cagcctatgg atcgtctttt agtaggcgat gtaggatttg gtaagacaga agtagctatg
     2281 agggcagcct ttaaagcggt gaatgatcac aaacaagtag ctgtcttagt tccaaccaca
     2341 gtcttggccc agcagcatta tgaaaacttc aaagcacgct ttgaaaatta ccctgttgag
     2401 gttgacgtct taagtcgttt ccgtagtaaa aaagagcaag ctgaaacact agaacgtgta
     2461 cgaaaaggtc aaattgacat tattatcgga acccatcgac tcctatcaaa agatgtggtc
     2521 ttttctgatt taggattgat tgtgattgat gaggaacaac ggtttggtgt taagcataaa
     2581 gaaactttaa aggaattaaa aactaaggtt gacgtcttaa ccttaacagc tactccgatt
     2641 cctagaacct tacacatgtc tatgttaggt atccgagatt tatcggttat tgagacccca
     2701 ccaaccaatc gttatcctgt tcaaacctat gttttggaaa ataatccagg tctcgttaga
     2761 gaagctatca ttcgtgaaat ggatcgtgga ggacaaattt tttacgttta taataaagtt
     2821 gacactattg aaaagaaagt ttcagagcta caagaattag tcccagaagc ttctataggt
     2881 tttgttcatg ggcaaatgag tgaaattcaa cttgaaaata ccttgattga ctttataaat
     2941 ggtgattatg atgtccttgt ggctacaaca atcatcgaaa caggagttga catttctaat
     3001 gtaaacactt tgtttattga gaatgctgat catatgggat tgtcaacttt atatcaacta
     3061 cgagggcgcg tcggaagaag taatcgtatt gcttatgctt acctgatgta tcgtcctgat
     3121 aaggtcctaa cagaggtttc tgaaaagcgt ttagaggcta ttaaaggctt tactgaatta
     3181 ggttcaggct tcaagattgc tatgcgagat ttgtctatac gaggagcagg taatatttta
     3241 ggggcttctc agagtggctt tattgattcg gtcggttttg aaatgtattc tcagttattg
     3301 gaacaggcta ttgctagcaa gcaaggaaaa acgactgttc gccaaaaagg taatactgaa
     3361 atcaatcttc agattgacgc ctatttgcca gatgattaca tatcagatga gcgtcaaaaa
     3421 attgacattt acaagcgtat tcgagaaatt caatcaagag aagattatct caatttgcag
     3481 gatgagctga tagatcgttt tggagagtat cctgatcaag ttgcctattt gttagagata
     3541 gctctgctaa aacattatat ggacaatgcc tttgcggaat tagttgaacg caaaaataat
     3601 caggtcattg ttcgatttga agtaacttct ttaacttatt tcttgactca ggattatttc
     3661 gaagccttat cgaaaaccca tcttaaagct aaaattagtg aacatcaggg gaaaatcgat
     3721 atcgtctttg acgttcgcca tcaaaaagat tatagaattt tagaagaatt gatgt
//
LOCUS       DAUGHY010000090         3769 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.090, whole genome shotgun sequence.
ACCESSION   DAUGHY010000090 DAUGHY010000000
VERSION     DAUGHY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.090"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            1..642
                     /locus_tag="VH752_001633"
     CDS             1..642
                     /locus_tag="VH752_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289962.1"
                     /translation="MNFFNFLLCVFKFTSEYLIKNWIALIALFLSYSNYRRNNLQVEL
                     IAAPVSDWILSVILDNGESIYNPNGTLRANIKIINPSNVDVSYFDLIVFDKNRKYQHY
                     YQKQNNIINDLTGREAIAAVQPDGNTILIEVPEADCGVLKAHSMTRMDLIIQTSEITD
                     RLFVAFKVAKKKKLFKANKAGYVNSPYQSFSASFPVELSKKPHYEDILKDLHE"
     gene            complement(741..899)
                     /locus_tag="VH752_001634"
     CDS             complement(741..899)
                     /locus_tag="VH752_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8289963.1"
                     /translation="MNWKKLMFGDLEYTFASRDGKEKTSIEFEGGVLPALLVLCGITW
                     LIAWFITK"
     gene            1286..2035
                     /locus_tag="VH752_001635"
     CDS             1286..2035
                     /locus_tag="VH752_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ8289964.1"
                     /translation="MASTIAFPAMVKELRLGKNLTMEQLAEELGKTKSTISKWEKGTR
                     SPKIYEIEEIAKFFGVEPKKMMFGDNPTSINPQVELIPSTLQKINSTSSQLEHSRQII
                     VLDTAETLLEQQKEIKNNEDTIAELFSYNYYDHAASAGTGQYLNDVQVEKIELPVDYD
                     ADFVIPVYGDSMEPKYHSGDYVFIKLSVELADGDIGVFEYYGDAYIKQLLINDEGAFL
                     HSLNGKYEDIPVDRDSDFRIIGEVVGSYSEK"
     gene            2061..2603
                     /locus_tag="VH752_001636"
     CDS             2061..2603
                     /locus_tag="VH752_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEQ8289965.1"
                     /translation="MNLDTVLQQMKECGAYDTWGTKKEVKALPEILSDDEIIQYATSG
                     FVNGNTVLVVLTQKRILFIDKGMLYGIRSTEIPLDMVNSVSYSKGLILGAIAVMNGAT
                     KIEISNIAKDTCPVLTEKIKDCVEQYKYNLYQHQSPQKTATVGSEDAVAEIRKFKKLY
                     DEGILTEEEFIAKKKIILGI"
     gene            2754..>3769
                     /locus_tag="VH752_001637"
     CDS             2754..>3769
                     /locus_tag="VH752_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ8289966.1"
                     /translation="MRKVAIYSRVSTINQAEEGYSIQGQIEALTKYCEAMEWKIYKNY
                     SDAGFSGGKLERPAITELIEDGKNNKFDTILVYKLDRLSRNVKDTLYLVKDVFTANNI
                     HFVSLKENIDTSSAMGNLFLTLLSAIAEFEREQIKERMQFGVMNRAKSGKTTAWKTPP
                     YGYRYNKDEKTLSVNELEAANVRQMFDMIISGCSIMSITNYARDNFVGNTWTHVKVKR
                     ILENETYKGLVKYREQTFSGDHQAIIDEKTYNKAQIALAHRTDTKTNTRPFQGKYMLS
                     HIAKCGYCGAPLKVCTGRAKNDGTRRQTYVCVNKTESLARRSVNNYNNQKICNTGRYE
                     KNTS"
BASE COUNT         1319 a          624 c          699 g         1127 t
ORIGIN      
        1 atgaattttt ttaatttttt attgtgtgtt tttaagttta caagtgaata tctaataaaa
       61 aattggatag ctttaatagc tctgtttcta tcttattcaa actaccgaag aaataactta
      121 caagtcgagt taattgctgc tcctgtttca gattggattt tgagcgttat tttagacaac
      181 ggtgaaagca tatataatcc aaatggtaca ttaagagcta acattaaaat catcaatcct
      241 tctaatgttg atgtaagcta cttcgacttg attgtttttg ataaaaacag aaaatatcag
      301 cattattacc aaaagcaaaa taatataatt aacgatttaa caggtagaga ggctatagcc
      361 gcagtacagc ctgatggcaa tacaatcctt atcgaggttc cagaggcaga ttgtggagta
      421 ttaaaagccc acagtatgac aaggatggat ttaatcatac aaacgtctga aatcacagat
      481 agactctttg ttgcttttaa agtagctaaa aagaaaaaac tatttaaagc taataaagca
      541 ggatatgtta attcacctta tcaatcattt tctgcgtcat tccctgtgga attatcaaaa
      601 aaaccgcact acgaggatat cctaaaagat ttgcatgagt gagagcagat tttcttgtgt
      661 gaaatatctt ggaagaacct agtacaccgt atttaatttt ttccatgcct tccctccttt
      721 ccactccttt tggggagttt ttattttgta ataaaccaag caatcagcca agtgataccg
      781 catagcacta acagcgctgg taatacgccg ccttcaaatt cgatgcttgt tttttctttg
      841 ccgtcacgac tggcaaacgt gtattctaga tcgccaaaca ttagtttttt ccaattcatg
      901 caacctctcc tttcattctt gcggagatac agccaatgtg ctaaactaaa cttaccccgt
      961 taggggagag ggcttcttag ccctcttgat attgtcacca ctctattgta gtgaacccta
     1021 agcttaaacc aaagaatctt gatttcgact tctagttctt tgtgtttagg ctttttgttt
     1081 agcctagatt tcatcagctg tacctccttt cgttttgctt aattccttaa gcttgattat
     1141 agtttaacac tgtgtttcct ttttgtcaac tattttgtgt taaaaaagtc aacttttttg
     1201 aatttgattt tttgctattt ttgttgacat tttgtaaaca tgtaattata atgtagataa
     1261 ttaagctata aaaaggagaa tttatatggc ttccactatc gcatttccgg caatggtcaa
     1321 agaacttagg cttggtaaga atttgactat ggaacagtta gcagaagaac ttggaaaaac
     1381 aaagtcaaca atatcaaaat gggaaaaagg gacgcgttct cctaaaatat atgagattga
     1441 agagatagca aaattcttcg gtgtagagcc taagaaaatg atgtttggag ataatcccac
     1501 ttcgatcaat ccccaagtcg aacttatccc atctactcta caaaaaataa actctacttc
     1561 ttctcaacta gaacacagta gacagataat tgttttagat acagctgaga ctttattgga
     1621 acaacagaaa gaaattaaaa acaacgaaga tactattgcc gaattatttt cttacaacta
     1681 ctacgaccac gcagcttcag ctggtacagg tcagtatcta aatgatgtac aagtagaaaa
     1741 aattgagtta ccagtcgatt atgacgcaga ctttgttatc cctgtttatg gtgattccat
     1801 ggaaccgaag tatcactctg gggattatgt gtttattaaa ctatccgtag agctcgcaga
     1861 tggcgatata ggcgtctttg agtattatgg tgacgcttat atcaaacagc tgcttataaa
     1921 tgacgagggg gcatttctgc acagtttaaa tggcaagtat gaagatatac ccgtagatag
     1981 agatagcgac tttagaatta ttggtgaagt tgtggggagt tattctgaaa aataagaata
     2041 tttttataag gagttttatt atgaatttag atactgtttt gcaacaaatg aaggaatgtg
     2101 gggcttacga cacttgggga acaaagaaag aagtaaaagc tttgccagaa attttgtcag
     2161 atgacgaaat tatccaatac gcaacatctg ggtttgtaaa cggcaatact gtccttgttg
     2221 tattgaccca aaaacgaatt ttatttatcg ataagggaat gttatatgga attagatcga
     2281 cagaaattcc tcttgatatg gttaatagcg tctcatactc taaaggttta attcttggcg
     2341 caatagctgt tatgaacgga gccacaaaaa ttgaaatatc taatattgcc aaggacactt
     2401 gccctgtact taccgaaaaa ataaaagatt gtgtagaaca atacaaatat aatctgtacc
     2461 aacatcaatc gcctcagaaa acagcaactg taggctcgga agatgccgtt gctgaaatcc
     2521 gtaagtttaa aaaactttat gatgaaggca tccttacaga ggaagagttt atagctaaga
     2581 aaaaaataat tttagggatt taaggtgtaa agttaaccgt taaaaaataa aaaagcccca
     2641 cgctctcaaa ctttggcgag tctgagcgtg aggcgaattc tagtatagta aaaacctgct
     2701 ttaagtaggt ctctttactg tactcatttt aacaaaaaat gaggtaaaaa acaatgagaa
     2761 aagtagctat ttactctaga gtatcaacaa taaatcaagc cgaagaagga tattccattc
     2821 aaggacagat tgaagcttta acaaagtatt gtgaggcaat ggaatggaaa atttacaaaa
     2881 actactctga tgcaggtttt tcaggcggta aacttgaacg accggcaata acagaattga
     2941 ttgaagacgg taaaaacaat aaatttgata ctattttagt ctataaacta gacaggctat
     3001 caagaaatgt aaaagacaca ctttacttag tcaaagatgt atttactgct aacaatatcc
     3061 attttgtcag cttaaaagaa aatatcgaca cttcttcagc aatgggaaat ctctttctca
     3121 ctctcttgtc agctattgca gagtttgaaa gagaacagat taaagagcga atgcagtttg
     3181 gtgttatgaa tagagcaaaa tctggaaaaa caacggcttg gaaaacacca ccttatggat
     3241 atagatacaa caaagacgaa aaaacattgt cggtcaacga gttagaggct gctaatgtca
     3301 ggcagatgtt tgacatgata atctctggct gctcaatcat gtcaattaca aattacgcga
     3361 gagacaattt cgtcgggaat acgtggacac atgtaaaagt aaaaagaata ttagaaaacg
     3421 aaacatataa aggattagtg aagtataggg aacaaacttt ctcaggtgat catcaagcca
     3481 ttatcgacga aaaaacatat aataaagcgc aaatagcttt agcacatagg acagatacga
     3541 aaacaaatac tagacctttt caagggaaat atatgctttc tcacatagca aaatgcggtt
     3601 attgtggtgc ccctttaaaa gtatgcacag gaagagctaa gaacgatggt acgagaaggc
     3661 aaacttacgt ttgtgttaat aaaacagaaa gtttggctag acgaagtgtt aataattata
     3721 ataaccaaaa gatttgtaac actggtcgat atgaaaaaaa cacatcgaa
//
LOCUS       DAUGHY010000091         3666 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.091, whole genome shotgun sequence.
ACCESSION   DAUGHY010000091 DAUGHY010000000
VERSION     DAUGHY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.091"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..1391)
                     /locus_tag="VH752_001638"
     CDS             complement(<1..1391)
                     /locus_tag="VH752_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEQ8289967.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSKVSH
                     EDLKRLKNHCLIDEFLQRKNKGFDFSAWHDGLITMGFDKLEEFDSLTIMNDTCFGPIW
                     EMAPYFENFEEKETVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQFWQSII
                     EYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKAESSFMPHPDFSYYNPTAILKHH
                     VPFIKVKAIDANQHIAPYLLNLIRETTNYPIDLIVSHMSQISLPDAKYLLSQKYLNCQ
                     RLAKQTCQKVAVHLHVFYVDLLDEFLTAFEDWNFHYDLFITTDSDIKRKEIKEILQRK
                     GKTADIRVTGNRGRDIYPMLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWRKELIDM
                     LVKPADSILSAFETNDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRKMDVKKQ
                     IDFQAMDTFVMSYG"
     gene            complement(1388..2395)
                     /locus_tag="VH752_001639"
     CDS             complement(1388..2395)
                     /locus_tag="VH752_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ8289968.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNETNIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTVYRMTTDSDSR
                     PTDTPKMIHRIQKLLDTQLNYLKKYNQVDTKEMSELLLQQDAKQEIRIHELSCFIQEL
                     QQTIVDKTKQQETREVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRK
                     K"
     gene            complement(2420..3625)
                     /locus_tag="VH752_001640"
     CDS             complement(2420..3625)
                     /locus_tag="VH752_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8289969.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDMYNDIVDFAELHHFMNQKLKNYSSGMQVRL
                     AFSVAIKAQGDVLILDEVLAVGDEAFQRKCNDYFMERKDSGKTTILVTHDMGAVKKYC
                     NRAVLIEDGLVKAYGEPFDVANQYSVDNTETAEDAMNAEKISVSDIAKDLKVSLISNP
                     RITPNDTITFEVSYEVLKDDFTYIAFSLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQ
                     CQLSQLNDIKLKLEVTVRDKNGQMLLFSTAEQSPQIVIQRNDIDQDDLSALDSASGLY
                     QRNGKWVFH"
BASE COUNT         1142 a          668 c          540 g         1316 t
ORIGIN      
        1 ccataactca taacaaatgt atccatagct tgaaaatcaa tttgtttttt aacatccatt
       61 tttctccaca aagacatcat ttcttgagcg attaaatgct cattccaagc attgacaatc
      121 ttattaaatc taaaaaaact tggaatatct gcgattataa taccaatatc atttgtctca
      181 aaagcgctaa gaatactatc tgctggtttc acgagcatat caatcaattc ttttcgccaa
      241 gattctcctg cccaaaaatc agcttctttt gattttttcg tatggaaatg accaatataa
      301 tcatattgag aaagtttatc ttttagtaat aacattggat aaatatctct tcctctattt
      361 cccgtaacgc gtatatcagc agttttacct tttctttgaa gtatttcttt gatctctttt
      421 cttttgatat cactatctgt tgtaataaaa agatcataat ggaagttcca gtcttcgaaa
      481 gctgttaaaa attcatctag caaatcaacg taaaaaacat gtaaatgaac agctaccttt
      541 tgacaagttt gttttgctaa ccgttggcag tttaggtatt tttgagataa aaggtactta
      601 gcatctggca aagagatttg cgacatatgt gaaacaataa gatctatagg ataattggtt
      661 gtttctcgta tcagattcaa taagtaagga gcgatatgct ggttagcatc aatagctttt
      721 acttttataa aaggcacatg gtgttttaaa attgctgttg gattataata tgaaaaatcc
      781 ggatgtggca tgaatgacga ctcagctttt ctagtatcaa agaccgtctg ataactaaag
      841 ccttcattta agagaatgga tgttaattgc gtttcataat gctgaatcac ttcttgaacg
      901 ttttcatatt ctattatcga ttgccaaaat tgttgaaaaa ctttgttttg aatgacttga
      961 tttttaaacg tcataaaata actttggaca tgttctttaa aagcttttgt acctctatta
     1021 ttagtaattc cccaaaagtc aactgtctct ttttcttcaa aattttcaaa ataaggagcc
     1081 atctcccaaa taggaccaaa acaagtatcg ttcataatcg tcaatgagtc aaactcttcc
     1141 aacttgtcaa atcccatagt aatcaagcca tcgtgccatg cagaaaaatc aaaaccttta
     1201 tttttgcgtt gcaaaaactc atcaataaga cagtggtttt tgagacgttt taaatcttcg
     1261 tgactcactt tactatttga gataaaaaca atcttagaaa acagtgatct catttgttcc
     1321 aattgatagt aaacatgtgc acttatttta ttgtatttgt tgaagtgaac atataataaa
     1381 atacgattca tttttttctc ctaataaaag caatgagctt agacgtgtat ttccattgac
     1441 gtgaattaat aatggcatgg tattgttgtc ttaattcaga tagttgattt ttttgttctt
     1501 ctatgacatt ttgcaactcc acttcacgtg tttcttgctg ctttgtttta tctacgatag
     1561 tttgttgtaa ttcttgaata aagcaactca actcatgaat tcgtatttct tgcttagcat
     1621 cttgttgtaa taatagttca gacatttctt ttgtatctac ctgattatac ttttttaagt
     1681 aatttaactg agtatcaagt agtttttgaa tacgatgaat catttttggt gtatcagtag
     1741 gtcttgaatc agaatcggtt gtcatgcgat aaaccgtcgt tgagtcttta atataggtca
     1801 gttgagtcag ttgaaataac tctagttgaa tgtcaaaagt atcgtctggt gtatctacat
     1861 tgattttttg gtttacttgt ctcattaaat cagcatcaac taaccatgtc gatgccatcg
     1921 tcatcccttt taaggctaaa actttttcgt aagtatctgt caaaggtgta tgaccagatg
     1981 aaaaagcatt agcatataat aagtcgcctt gactatttac aaagtcaaaa tcagtattac
     2041 accatcgtga ccgtttggaa gcttctaaga catcaacttg tttttggagt ttaaaactat
     2101 ccgtccagta atcatcacca tcacatctag caatatattt gccttttgca taaaggcatg
     2161 cttttatcca agtctttgtt attccaatat tagtttcgtt aaagagtaat ttaatttttc
     2221 ctgaagactt tttttgataa ctctttaaaa tctctcgtga gtcatctgta gaagcatcat
     2281 caataacaat aatttcaacc tcaaaatctg tcacttggct taaaaaactg tcaagtgcat
     2341 cagatatcca aggagctttg ttataattgg tgcaaataat tgaaacttta tacataaatt
     2401 cccttctagc tattactcat taatgaaaaa cccacttccc attacgttga tacaagccac
     2461 ttgctgaatc tagagctgac agatcatctt gatcaatatc gtttctttga atcactatct
     2521 ggggtgattg ttctgctgtt gaaaagagta gcatctgacc gttcttatct cggacagtta
     2581 cttctaattt tagttttata tcattaagtt gagatagttg acattggtag gaaatgcatt
     2641 ttctacctat accttgtgtt ggataatcta aagaattgtc gttgtaaacc caaatgtttc
     2701 tatcaatatc cgttaaagaa aaggcaatgt aggtaaaatc atcttttaaa acttcataag
     2761 aaacttcaaa tgtgattgtg tcatttggtg ttattcttgg atttgagatt aatgacactt
     2821 tcaaatcttt ggcaatatca gatacagata ttttttcagc attcattgca tcctcagctg
     2881 tctctgtatt atctacacta tactgattag caacatcaaa tggttctcca tatgctttaa
     2941 caagaccatc ttcaataaga actgcacggt tacaatactt cttaacagct cccatatcat
     3001 gtgtcactaa aatagtcgtc ttaccactgt cttttctttc cataaaataa tcattacatt
     3061 ttcgctgaaa agcttcatct cctacagcaa gcacctcatc taaaataaga acgtcacctt
     3121 gggctttaat agccactgaa aatgctaaac gtacctgcat gccagaagaa taatttttta
     3181 atttttgatt cataaaatga tgcaactctg caaaatcaac aatatcattg tacatatcat
     3241 caacttcgtc tttggtaaat cccagcatgg cgccgttcat atagacgttt tctcgtcctg
     3301 ttaattctgg attgaatccc actccaagct ctataaaaga aaccatcttc ccatctactg
     3361 taacctgtcc tttttctgga acatagatct gggaaataat tttaaggagc gttgattttc
     3421 ccgagccgtt acgcccaaca attccaaaaa aatcgccttt ataaacatca aagttaatat
     3481 cttttagcac atgttgttca gtaaatcctt taatccctct taggcgatta accagagtcg
     3541 tgcgaaaact tttagttgct tctgtaggca gtttaaatgt tttactaaca tgctgtactt
     3601 ttactgcaat ttctctatta atcattaaat aatctccgca aatctatcgg catttttctt
     3661 aaagac
//
LOCUS       DAUGHY010000092         3390 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.092, whole genome shotgun sequence.
ACCESSION   DAUGHY010000092 DAUGHY010000000
VERSION     DAUGHY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.092"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..719)
                     /gene="recO"
                     /locus_tag="VH752_001641"
     CDS             complement(<1..719)
                     /gene="recO"
                     /locus_tag="VH752_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEQ8289970.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAAI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFSHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTF"
     gene            complement(905..1867)
                     /locus_tag="VH752_001642"
     CDS             complement(905..1867)
                     /locus_tag="VH752_001642"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ8289971.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMGLQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
                     GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVL
                     DTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN"
     gene            complement(2120..3316)
                     /locus_tag="VH752_001643"
     CDS             complement(2120..3316)
                     /locus_tag="VH752_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ8289972.1"
                     /translation="MKKRILSAVLVSGVTLGAATTVGAEDLSTKIAKQDSIISNLTTE
                     QKAAQNQVSALQAQVSSLQSEQDKLTARNTELEALSKRFEQEIKALTSQIVARNEKLK
                     NQARSAYKNNETSGYINVLLNSKSISDVVNRLVAINRAVSANAKLLEQQKADKVSLEE
                     KQAANQTAINTIAANMAMAEENQNTLRTQQANLVAATANLALQLASATEDKANLVAQK
                     EAAEKAAAEALAQEQAAKVKAQEQAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPA
                     ALTAPASPSARPQVSYSSVNNYPVGQCTWGAKSLAPWAGNNWGNGGQWAASAQAAGYS
                     VGSTPMVGAIAVWNDGGYGHVAVVVEVQSSSNIRVMESNYSGKQYIADHRGWFNPTGV
                     TFIYPH"
BASE COUNT         1065 a          674 c          554 g         1097 t
ORIGIN      
        1 atgaatgttt tactttttaa tttgattcct acatagtcgt gatacaactc atcaatgaac
       61 tgacgaagct tctttttgat gtctttattc aatgaaatag ttctcaaatc atcaaatgtg
      121 attttttgaa atcgactcaa caagtagata acatttggat ctaaatgatt acgtcggttg
      181 tctttgtaat aatgttcaga acaaagtaca gctgaaaaac gatgggaaaa atcaagcggt
      241 aaatcagtgc gatgacaaat ggcacactca tgaaagttta gactaatacc aaaacgatct
      301 aatatctgaa tttcaaaaat atttgtcaaa atttcataat ctaggccctc ttccatcaaa
      361 tcaagtgttt ttttcaaaaa cgtaaacaaa tgcgaatctg attcattatc tgcaatcgca
      421 gcatcagcta atgctaagac ataactagca taggctaatc gaaaaatatc attattaata
      481 taccgataag tgttaacgtt actatagtca acaacataag acaagcctgt atcatttaac
      541 ttgaaaataa aatcagcaat cgttagtggt tgaatgattg aggacatttt ggaacgacta
      601 atatgtttca cgaaaaacat ctgcttacct gctacttcag taaatatttt gactaattta
      661 tcatcttctc gataattcct attaaaaaga acgatgccta gtgattctgt tagttgcata
      721 ttacctcgtc gtatttttct cttacctttt atcttagttt acaataaact gttgttatct
      781 ttcttattat acccaaaatc ctttagaatt gcatagttca tcccacaata attaatacca
      841 cattaccctt acattttatt caagtcaaaa agcctgagat ctattatctc aggcttcgtt
      901 aagtttaatt gcccatctca aataaaggtg acaacggacg tttttcatga atgcgaataa
      961 tcgcttctgc aaccaaatca gcaattgaaa tttgttcaat tttatcaata aggcgttctt
     1021 ttggcagata aatagtatca agaacaatta gtttttcgat agccgaacgt tgaatattat
     1081 caagtgctgg tccagaaaga acgggatgcg tacatgacgc atacacagct gttgcccccg
     1141 cttcagcaag cgcatctgct gcatggcaaa tagtccctgc ggtatcaatc atatcgtcaa
     1201 tcaagataca tttcttgccc gaaacatttc cgataatatt catcacttca ctggtgttca
     1261 tcttatccac actacgacgc ttatcgataa ttgcaattgg tgtttggagg aattgtgcta
     1321 atttacgcgc acgagtcaca ccaccatggt caggactaac aacgacaaca tcttcaccca
     1381 ctaaaccatg acgatcaaaa tagtctgcaa ttaaaggtgc acccatcaag tgatcaactg
     1441 gaatatcaaa gaacccttga atttgagcag catgtaaatc aaccgttaat aagcgatcta
     1501 caccagcaac ttctaacata ttagcaacta attttgaagt aataggttca cgagaacgcg
     1561 ctttacggtc ttggcgtgca tagccgtagt aaggcatcac aactgaaatt ttttcagcac
     1621 tagcacgctt taaagcatcg accataatca aaatttccat taaattgtca ttgacaggtg
     1681 agctagttga ttgcaaaata aagacatgat gaccacgaat tgattcttca atatttactt
     1741 ggatttcacc atctgaaaac tggcgaaccg tagactttcc aagttgaaga cccattgcag
     1801 acgcaacttt ttctgctaat tccttgtttg acgataaagc aaacaatttt aaatcagaat
     1861 aagacatttt ttcctccaaa gttttaatcc tataccattt taacgctttt caagtgaatt
     1921 atcaaatgaa attgaaaaac cgttaaagaa tgtattaaat atacctttta tcgactttat
     1981 atacttctct tatattatac ataagcgatt ggccaaacaa ctctcttata aattaaccat
     2041 atcgtcaata ctctcagaac tattccacaa taggagtaat caaaaaggtt agaacaactg
     2101 ttctaaccta aataatatgt tagtgtggat aaataaatgt aacacctgtt ggattaaacc
     2161 aaccacggtg gtcagcaata tactgtttac cactgtagtt tgattccatc actcgaatat
     2221 ttgatgaact ttgaacttca acaactactg cgacgtgacc ataaccgcca tcattccaaa
     2281 ccgcaatagc tcctaccata ggtgtagatc ctacagagta gcccgctgct tgagcgcttg
     2341 cagcccattg tccaccgtta ccccaattgt ttcctgccca tggagctaat gatttcgcac
     2401 cccatgtaca ttgtcctact gggtaattat taacagaact atatgaaact tgtggtcttg
     2461 cagaaggaga tgccggagcc gtgagtgcag ctggttcaat agcattacta ctttgcgctg
     2521 ccggagtagc ttgtggtgct ggagtaatag cagattttgc tgcttcaaca gatgctgctt
     2581 gttgtgcagc ctgttcttgt gccttaactt tagcagcctg ttcttgtgct aaggcttcag
     2641 cagcagcttt ttctgcagct tctttttgag ctaccaaatt agctttatct tcagtagcag
     2701 atgctaattg gagagctaaa tttgcagttg cagctaccaa attagcttgt tgagtacgta
     2761 atgtattttg gttttcttca gccattgcca tattagcggc aatggtatta atagctgttt
     2821 ggttagcagc ttgcttttct tcaagggaaa ctttatcagc tttttgttgt tctaacaatt
     2881 tagcgttagc agagacagct ctattaattg ctactaaacg gtttacaaca tcagaaattg
     2941 atttagaatt caaaagtaca ttaatataac cagaagtttc attgttttta taagcactac
     3001 gagcttgatt ttttaatttt tcattacgag caacaatttg acttgttaga gccttaattt
     3061 cttgctcaaa tcgctttgaa agcgcctcaa gttctgtatt tcttgcggtc agtttatctt
     3121 gttcagattg tagtgaactt acttgagcct gtaacgctga aacttgattc tgtgcagctt
     3181 tttgctctgt agtcagattt gagataatag aatcttgctt agcaatctta gtacttaaat
     3241 cctccgctcc tacagttgta gctgctccga gggtaacacc acttacaaga actgctgata
     3301 aaattctttt tttcataata aaaaaactac tccttttcga taagacatct tatattttac
     3361 caaaaaaaaa agtatttaat attacgcaat
//
LOCUS       DAUGHY010000093         3119 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.093, whole genome shotgun sequence.
ACCESSION   DAUGHY010000093 DAUGHY010000000
VERSION     DAUGHY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.093"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..285)
                     /locus_tag="VH752_001644"
     CDS             complement(<1..285)
                     /locus_tag="VH752_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEQ8289973.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLEKIPNSGDGFPLKITINKELTGFKLAITDQSGLRLVNIFKTKDNQILQD"
     gene            complement(275..1402)
                     /locus_tag="VH752_001645"
     CDS             complement(275..1402)
                     /locus_tag="VH752_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ8289974.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     HTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            complement(1399..2379)
                     /locus_tag="VH752_001646"
     CDS             complement(1399..2379)
                     /locus_tag="VH752_001646"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ8289975.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAAEVLETAPIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            complement(2519..3097)
                     /locus_tag="VH752_001647"
     CDS             complement(2519..3097)
                     /locus_tag="VH752_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEQ8289976.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLSQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFANSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHIAFKYAKSKVARCINTLKKFNDK"
BASE COUNT          951 a          548 c          593 g         1027 t
ORIGIN      
        1 atcttgaagg atttgattat cttttgtttt aaaaatatta actaatctaa gaccagattg
       61 atcggtgata gctaatttaa aacctgtgag ttctttatta atagtgattt ttaaagggaa
      121 accatcccct gaattaggta ttttttcgag taaacgtatt aattggtagt gccctacttt
      181 agtttgacta aatgtagtat ctcttagggt atatttttta atgtttggac tgattgtata
      241 aaggtattta cagtctttaa gcttaatttc tttttcaaaa gccatctgtt cctcctaaaa
      301 tattttttaa tgtttgagct aattgattaa catcttcaat gctattttga tctgaaaaac
      361 tgatacgaat tgattctttt aaacgagaag agtcatcccc atagtacgca gctaaaacat
      421 gacttggatt aacagctcca gcagtacaag cagaaccagt tgatactgca atgcctgcta
      481 agtcaagttg tgttagtaaa atagtatttt ggtagcctaa aaatccaata ttgagaacat
      541 gaggaagata atgagtacct tgattaatat agtagggtag tccttctagg aggctaatca
      601 agtgatgacg taaactgata atgtgatcag ttgattggtc aagacaagtc atagcatcag
      661 tcaaagcttg tgccatacca ataataccta acatattttc agtacttgcg cgtcgctttc
      721 cttcttgatc acccccgtga agaagagggt cgattggttg tccgttactg tataaaaagc
      781 cgcatccttt aggaccatga aatttgtgag cagaagcaga taaaaagtca atcccaagtt
      841 cgctaggaat aatcttgagt ttgccaacag cttgaacggc atcaacatga aaagcagctt
      901 gatggtcttt gagcagattc ccaatgtcct taataggtaa caggtcacct gtttcgttat
      961 tggcatacat tatgcttacc aaaatggtgt catctctcaa ggcttgtttt aagtcagata
     1021 aatttatttg tccattttga caaggcaaat aagtgacctc aaaaccaaat cgctcttcga
     1081 gataggccat ggtatgaagc acagagtggt gctcaatagt agttgtgatg agatgtttgc
     1141 cctttgcttg atgagctaaa gcatagcctt ttatggccat attattgctt tcagttccac
     1201 cggacgttac tataatttgc tgttcacttg cccccaagtt tctagcgata gcttgtcggc
     1261 attcacgaag gattttattg gctcggcgcc cgtagaaatg aatactagag gggttaccaa
     1321 agttatcttg catagctgct gtcatcgccc taatcacatt aggactaagt ggtgtggtgg
     1381 cggcgttatc aaagtaagtc atgcgttatt tttgccttta ggttgataag aaaaaagtgg
     1441 acttaagggt cttctttcat ggatgcgaat aatagcttct gcgattaaat cactggcgct
     1501 aagataagta acgttttctg gcacacgatt tttagtttta acagaatctg taacgataat
     1561 ttctttgatt ggagctgttt ctaatacctc agcagctcca ccagcaaata agccatggct
     1621 agcgaccgcg taagtatcag tagcgcctga gcgttcaaga attttagcag cttctgcaaa
     1681 ggttttccca gtatttaaaa tatcatcaat taaaatggct tttttaccag aaacatcacc
     1741 aataatataa ccttgttcac gttcagaatc atcttgtgca tagtcgataa tagcaatcgg
     1801 ggaatcaaga tattcagcca agcttctagc acgtttaatt ccagaatttt tagggctaac
     1861 gacaacaaca tcagaacctg ataagcctaa tttactgtaa cgttctgcaa aaagagggac
     1921 tgtaaagagg ttatccactg gaatatcaaa aaaaccttgt acctgaacag catgtaagtc
     1981 aagcgtcact acacgatcaa taccagcttt agtcagcata ttagctacta gtttagcagt
     2041 aatcggttcg cggggcttag cgacacggtc ttgacgagag taaccaaagt atggcaatac
     2101 aatattgacg gtattagcac ttgcacgttt acaagcatca atcatgatga gtaattccca
     2161 aagattatca ttgacaggaa aactagtgga ttgaataata tagatatcat ctccacgcac
     2221 tgtttcttca atattgatca taatttctcc atcggaaaat tgacgtgaag acatttttcc
     2281 aagagggatt ccagcagctt tagcaatttt ttcagcaatt ggaagatttg atgtgagtga
     2341 gaacagtttg atttgcttgt cagcatatcg ttcagtcatg atagatttag ctccttaact
     2401 tcaataaaat tgggtaaact gttttattaa tgagtcacat tacccaaaag tgtttaattg
     2461 acatccctat tttaacagaa aaacagaaaa atttcagttt ttctgtcgct tttagccatt
     2521 atttatcgtt gaactttttt aaggtgttaa tacaccgagc tactttgctt ttagcatatt
     2581 tgaaagctat gtgatgttca gataaaaaag aatcaaaatc aatctttcct tgaagagcgt
     2641 cacttacttc taattctaat tcatagtctt tggtattggc atataggtta tagtctaagg
     2701 ccaatttacc tatcggtatc actgtctcgc gacgaacggt agttaaattg ccaaaagtta
     2761 ccagtgcaga tggtttgata ccttttgaaa taataatatc aagagcagtt gattcaggaa
     2821 gattcccatg tttaatcata tcttttgctt gttcaagaaa aagagggaca ttgtactcac
     2881 gattccccac tttttctggc acttttaagg ttaattcggc gctatttgca aatgttcgaa
     2941 tacgtaatga cattttgtta gcttttaaat caaatgcttt tgtatcaata tagtaattag
     3001 tttgtgtaac aggcgtcaca tgtttcattt gagataaaag acggttatac tcattttttg
     3061 taagtagagt tttatattcg atttctaggt tagtcatggg aaattccttt taatttgtg
//
LOCUS       DAUGHY010000094         2812 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.094, whole genome shotgun sequence.
ACCESSION   DAUGHY010000094 DAUGHY010000000
VERSION     DAUGHY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.094"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            80..730
                     /locus_tag="VH752_001648"
     CDS             80..730
                     /locus_tag="VH752_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ8289977.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNTEKDYILNAKPNGYRSYHLILELETHFPDILGGKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
     gene            733..1401
                     /locus_tag="VH752_001649"
     CDS             733..1401
                     /locus_tag="VH752_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8289978.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIMMPIKSGIEALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFL
                     MLNQGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVFIAGDKDTSYC
                     LLEK"
     gene            1410..2642
                     /locus_tag="VH752_001650"
     CDS             1410..2642
                     /locus_tag="VH752_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ8289979.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPGTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSMMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSAIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVVI"
BASE COUNT          930 a          406 c          566 g          910 t
ORIGIN      
        1 gttatatagt tagaaataaa gataaccgat cattgcagag atttaagcat ttgttgagaa
       61 aatttgttat aataagcgta tgaaagaaag actagaacat tattctacca gctctatata
      121 tagcggattt gaagtgtact tgccgttggt actacagaca ataacagatg tgattattgc
      181 tgagaatatc aaaagcaaaa aagaaacggg gtttaaacta tacgaacact ttacatcccg
      241 tattaaatcg gaagcttcga tgattgaaaa atgtcaacgc aaacagcttc ctctaacaag
      301 caaatctgcg ttaaaaatca ttaaagatag tatcggtatt cgcattattt gtggttttat
      361 tgatgatatt tatcggatgg tagacttgct caaaagcatt cctggtatgt cagttaacac
      421 tgaaaaagat tatattttaa atgccaagcc taatggctat agatcatatc atctcatttt
      481 ggaattagaa acacattttc cagatatttt agggggaaaa aaaggatgtt attttataga
      541 agtgcagttg cgaaccattg ctcaagactc ttgggctagc ttggaacatc aaatgaagta
      601 caaacatcaa gttgctaatg cagaaatgat tacaagagag ttaaagcgtt gtgcagacga
      661 gttggcatct tgtgatgtta ccatgcaaac cattcgtcaa ttgattcaag aaacgacgga
      721 ggaagagtaa ccatgaaaat tttgttagct gaagacgaat ggcagatgtc taatgttttg
      781 acaactgcca tgactcatca aggttatgac gttgatgtgg tttttaacgg tcaagaggca
      841 attgataaag ctaaagacaa tgcatatgat attatgattt tagatatcat gatgccgatt
      901 aagtctggca ttgaggcttt aaaggagatt cgcgcttcag gaaattgctc acatattatt
      961 atgctgacgg ctatggcaga aattaatgac cgtgttactg gactagatgc gggagctgat
     1021 gattatctga ctaaaccgtt ctcattaaaa gaacttttag ctagattaag gtcgatggaa
     1081 agacgagtag aaagttttac tcctcaggta ttacaatttg ctggtgtcac attaaatatt
     1141 aatgagcaag aattgagtgc cggtaatgct atacgattag cttctaaaga aggtaaatta
     1201 atggcctttt taatgttaaa tcaaggaaag tacttggata ctaaaacact ttaccaacat
     1261 gtttggtcag accaagaaga ttatgatact tctattgttt gggtttatat ttcttatctt
     1321 cgtcaaaaat tattagctat tcaagcaaat gtttttattg caggtgataa agatactagc
     1381 tattgtttat tagaaaaata ggaggtgaca tgtttaatcg tatccgtatt cgttttatca
     1441 tgattgcttc tattgccatt tttattattt taagttcaat tgttggtatt attaacacgg
     1501 cacgctgtta ccaaagtcaa caagaaatta accgtatttt acatttaatt tcctcaaata
     1561 aaggtaaatt acctggcact acggaaagct ctaaacgact tggaacgaaa ctgtctgaag
     1621 atagcctaag ccagtttcgg tattatagtg tcatttttaa tgctaatgga catctacttt
     1681 cttctaatac tgctaatatt tcagctttag atagagaaga agcccaatat tttgctagac
     1741 tttttgctaa gtctggggaa gaaaaaggca gttaccgtca ccaagatagt gtttattcgt
     1801 acttgattac tcagctgcca aatgaagaaa aactggttgt tattttagat actacctttt
     1861 attttcgtag tgtaggagat ttattagccg tttcaatgat gttggccttt ggaggattta
     1921 tcttttttgt tgttttagtg agtctttttt ccggtatggt catcaaacct tttgttcaaa
     1981 attatgagaa gcagcgccgc tttattacta atgcaggcca cgagttaaaa acaccactag
     2041 ccattatctc agccaataat gaattggtcg aattaatgac tggagaatca gaatggacca
     2101 agagtacaag tgatcaggtg aagcgattga caggcttgat taatcaaatg attactttgg
     2161 ctcgtttgga ggaacagcca gatgtggtat tacatatggt agacttttca gctattgctc
     2221 aagatgcagc tgaagatttt aagagtctgg ttttgaaaga tggtaaacgt tttgatttga
     2281 cgattcagcc gaatattatg attaaagcgg aggaaaagtc attatttgaa cttgtgacaa
     2341 ttttagttga taatgccaat aaatattgtg atcctaaagg cttggtcaag gtatctttaa
     2401 ccactattgg aagaagacga aaacgagcga aattagaagt ttctaatacc tatttggaag
     2461 gaaaatccat tgattacagc cgcttttttg aacgctttta tcgcgaagac gaatcccata
     2521 atagcaaaga aaaaggttat ggaattggtt tatctatggc agaaagtatg gttaagttat
     2581 ttaaaggaac gataactgta aattataaaa acgatgctat tgtttttaca gtggtgattt
     2641 gacttaaaca tttttcagta cctttagcta gtaataatgt ctaggtaaag gtactaaacg
     2701 atgcttaacc agcccatgag gctggttttt aacgtttaat ttagtagaag agtgatgaag
     2761 agtgattaaa gaaggtagtt tgaaataaga aaatttttgt tatgatccta aa
//
LOCUS       DAUGHY010000095         2798 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.095, whole genome shotgun sequence.
ACCESSION   DAUGHY010000095 DAUGHY010000000
VERSION     DAUGHY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.095"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..831
                     /gene="dnaN"
                     /locus_tag="VH752_001651"
     CDS             <1..831
                     /gene="dnaN"
                     /locus_tag="VH752_001651"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEQ8289980.1"
                     /translation="QVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSII
                     AETAFAASLQESRPILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIP
                     SKSLREFSAVFTDDIETVEVFFSPSQILFRSEHISFYTRLLEGNYPDTDRLLMTEFET
                     EVVFNTQSLRHAMERAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQ
                     SGSDLTISFNPTYLIESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN
                     "
     gene            906..1103
                     /locus_tag="VH752_001652"
     CDS             906..1103
                     /locus_tag="VH752_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEQ8289981.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            1433..2548
                     /gene="ychF"
                     /locus_tag="VH752_001653"
     CDS             1433..2548
                     /gene="ychF"
                     /locus_tag="VH752_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEQ8289982.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNILQKIKPVLEDGKSARTIEFTEDEAKVVKGLFLLTTKPVLYVANVDEGKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMAYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            2618..>2798
                     /locus_tag="VH752_001654"
     CDS             2618..>2798
                     /locus_tag="VH752_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEQ8289983.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYF"
BASE COUNT          958 a          421 c          546 g          873 t
ORIGIN      
        1 caagttgttt taaccagtgg taaatcagag attaccttaa aaggaaaaga tgttgaccag
       61 tatcctcgtc tacaagaagt atcaacagaa aatcctttga ttttaaaaac aaaattattg
      121 aagtctatta ttgctgaaac agcttttgca gccagtttac aagaaagtcg tcctatttta
      181 acaggagttc atattgtatt aagcaatcat aaagatttta aagcagtagc gactgactct
      241 catcgtatga gccaacgttt aatcactttg gacaatactt cagcagattt tgatgtggtt
      301 attccaagta aatctttgag agaattttca gcagtattta cagatgatat tgagaccgtt
      361 gaggtatttt tctcaccaag ccaaatcttg ttcagaagtg aacacatttc tttttataca
      421 cgcctcttag aaggaaatta tcccgataca gaccgtttat taatgacaga atttgagacg
      481 gaggttgttt tcaataccca atcccttcgc cacgctatgg aacgtgcctt cttgatttct
      541 aatgctactc aaaatggtac tgttaagctt gagattactc aaaatcatat ttcagctcat
      601 gttaactcac ctgaggttgg taaggtaaac gaggatttag atattgttag tcagtctggt
      661 agtgatttaa ctatcagctt caatccaact taccttattg agtctttaaa agctattaaa
      721 agtgaaacag taaaaattca tttcttatca ccagttcgac cattcaccct aacaccaggc
      781 gatgaggaag aaagttttat ccaattaatt acaccagtac gaacaaacta agtaagaaaa
      841 agctcccttt taggagtttt tttgttatta taaatattaa cgataatata agtggagaaa
      901 agggaatgta tcaaattgga tcatttgttg aaatgaaaaa acctcatgct tgtgtaatta
      961 aagaaactgg taaaaaggct aatcaatgga aggtactgag agtaggagct gatattaaaa
     1021 ttcagtgtac taactgtcag catgtgatta tgatgagtcg ttatgatttt gagcgaaaat
     1081 taaaaaaagt actgcaacca tgaaagtcct tttaaataaa taattctagc tatatttgca
     1141 accaatactt tcctaaaaaa ttgctagtat gccgttggaa aattagctat tctaatgtta
     1201 tcgaaagaag aaaggtggcc attgacaatg acgaaagttg tagcacagtt acttagtttc
     1261 tcggcaaact atatcaaaat gagaataata caccaaactt tgataattta aatgaaggtg
     1321 aaagagtggc ataatgataa gcaaatctga aattttcttc ttattcatac cgctttttct
     1381 tctatttttt ggtataataa tcttgattga aatttgaatg gagattcgct aaatggcttt
     1441 aacagcaggt attgtaggct taccaaatgt tggtaaatca accttattta acgcaattac
     1501 aaaagcagga gcagaagctg ctaattatcc ttttgcgact attgatccca atgttggtat
     1561 ggtagaggtt ccagatgagc gtctgcaaaa attgacagaa ttgattacac ctaaaaaaac
     1621 agttccgaca acctttgaat ttactgatat tgcaggtatt gttaaaggtg cttctagagg
     1681 ggaaggtcta ggaaataaat ttttagctaa tattcgtgaa gtcgatgcta ttgttcatgt
     1741 ggtacgtgct tttgatgatg aaaatgtcat gcgtgaacaa ggtcgtgagg atgctttcgt
     1801 tgatcctata gcagatattg acactattaa tcttgaatta atcttagctg atttagagtc
     1861 aatcaataaa cgttatgcgc gtgttgaaaa aatggcacga actcaaaaag ataaagaatc
     1921 agtagcagag ttcaatattt tgcaaaagat taaacctgtt ttggaagatg ggaaatcagc
     1981 taggacaatt gagtttacag aagatgaagc aaaagttgtt aaaggtctct ttttattaac
     2041 aactaaacct gttttatatg tagctaatgt cgatgaaggt aaagttgcta atccagatgg
     2101 tattgattat gtcaaacaaa ttcgtgactt tgcagctact gaaaatgctg aagtagttgt
     2161 tatctcagcg cgtgcagaag aggaaatttc agagcttgac gatgaggata aagaagaatt
     2221 tttggaagct atcggtctta ctgaatcagg cgttgataaa ttaaccagag cagcttatca
     2281 tctcttaggc cttggaacct attttacagc aggtgaaaaa gaggttcgtg cttggacgtt
     2341 taagcgtggt ataaaagctc cacaagctgc tggtattatc cattcagatt ttgaaagagg
     2401 ttttattcgt gcagtaacca tgtcttatga tgatctaatg gcctacggtt cagaaaaagc
     2461 cgtcaaagaa gctggacgct tgcgtgaaga aggaaaagaa tatgttgtcc aagatgggga
     2521 catcatggaa ttcagattta atgtgtaatt atattaaaac aatatcagaa ggttggaaga
     2581 acattccagc ccttttggca ttttagaaag agaaaatatg gtaaaaatga ttgttggtct
     2641 gggaaatcca ggctctaaat atgaaaaaac aaagcacaat attggtttta tggctattga
     2701 caatattgtc aagaaccttg acgttacctt tacagatgat aaaaatttta aagcacagat
     2761 aggaagtact tttattaatc atgaaaaagt ttactttg
//
LOCUS       DAUGHY010000096         2751 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.096, whole genome shotgun sequence.
ACCESSION   DAUGHY010000096 DAUGHY010000000
VERSION     DAUGHY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.096"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..395
                     /locus_tag="VH752_001655"
     CDS             <1..395
                     /locus_tag="VH752_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8289984.1"
                     /translation="QSTPHLKVVMLTGYVKSIYRERAKKVGAYGFVDKNIDPKQLISI
                     LKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEETLQISRRTVFNHL
                     THIYSKLLVNNKQEAIYKAEQLGYFMDF"
     gene            complement(947..2527)
                     /locus_tag="VH752_001656"
     CDS             complement(947..2527)
                     /locus_tag="VH752_001656"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEQ8289985.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLIAVCDNLEEHFNTFTDAQKIFEDAYQDQGLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTISNVMKT
                     LNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGREDQK
                     GMTLYDPAMGSGSLLLNAKKYSHQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDSHIKKVLNAYKSRDNSDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLSDIDQEIAKTNAKLDQLMK
                     QLVGTTKEAQDELDTFRN"
     gene            complement(2540..>2751)
                     /locus_tag="VH752_001657"
     CDS             complement(2540..>2751)
                     /locus_tag="VH752_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ8289986.1"
                     /translation="GIIKIAQGGTQIYVNYSSVEQLKINIPTLKEQEAIGNFFQTLDQ
                     QIAQSEEKLTELKALKQTLLNRLFV"
BASE COUNT          859 a          428 c          524 g          940 t
ORIGIN      
        1 tacagtctac tcctcatttg aaagttgtta tgttgacagg atatgttaaa tcaatctata
       61 gagagagggc aaaaaaagta ggagcatacg gttttgtaga taaaaatata gaccctaaac
      121 aactcatttc tatcttaaaa aaagtggatt ctggaaaaaa gtattttgaa caaatcgaat
      181 ctcaggatta tgttgaatct ttaactgacc aggaaatagc tatcttaaat ttgagtaaga
      241 aaggattctc tataaaagaa atagaggaaa cgttacaaat tagtaggaga acagtattta
      301 atcatttgac ccacatctac tcaaagcttt tggtaaataa taagcaagag gctatctaca
      361 aagccgaaca attagggtat tttatggatt tctgagttgc agataatcaa acgacataag
      421 ttttacttgt gtcgttgcta gggatttcct gttaaatacc cagatttaaa ctcattgttt
      481 ttgagttctt tttgattgaa attgattgag ataacgcttt tataaaaagt agggcaatgc
      541 ctgatatgaa aagcttttta aatttagtca aacaaaagtt gtttaaacca ggtctaaaaa
      601 aactcgtaaa gcttcacaac tcccagaacg ttaatatatg cttatatatc aacgattgga
      661 actactttgt ggttcgcacc atggtttttg tggaaggatc aaaagttgtc ctgaaaattt
      721 ctcttaaccg tgtttaagct ttctaaacgt ttgagaaatc attgtaactt caaataacta
      781 ttttaaataa aacaaagaaa cggacaaagt tagatgagat gattctattt tgatttatag
      841 gtgaagaagg agaatcttgt ttgataagta ctcaataaag gttcactact caattttaaa
      901 taaaacgcca cagtagtaat catctactgt ggtgtttttt ggtcttttag tttctaaaag
      961 tatccaattc gtcttgtgct tctttggttg ttccgactag ttgtttcatg agttgatcta
     1021 gtttggcatt tgttttggca atttcttgat caatgtcact gagctgttta gctaattctg
     1081 gcagtggttt gacggggact tcctcaaagg tatcgacata gcgtgggata tttaagttat
     1141 agtcattttc aatgatttca tcaaatgagg ctaaatatga aaacttatca gaattatcac
     1201 gactcttata ggcattaagg acctttttga tatggctatc tgtcattgtg ttttgatttt
     1261 tgcctttatc aaactctttt gaagcatcga taaagaagac atctttgttg gttctgttct
     1321 tctttaatat gataatagtc gtagggatgc ttgtattata aaagatattt gatgggagtc
     1381 cgataatggt atcgatagcc ccttgttcga gtagtttttg gcgaatcttg ccttctgcag
     1441 caccacggaa aaggacaccg tgtggtaaga caatcgccat agttcctgta ttttttaaat
     1501 gatagaaacc atgaagtaaa aaggcaaagt cagcttttga ttttggtgct aatacaccat
     1561 aacttgaaaa ccgtggatct gttaagaaac cagcagtcgc tgaccatttc aaagaataag
     1621 gtgggttcat caacacccca tcaaagttgg ttggttcatc tgttggccag tctgcatcca
     1681 aggtatcagc gttacttaag tgttggtttt cgatggcaac gccatgaagc atcatgttca
     1741 tacgagccaa gttataagta gaagtattga tttcttgacc atagtaagaa acggtatcgg
     1801 attgatgact atatttctta gcatttaaaa ggagtgatcc agatcccata gcagggtcat
     1861 agagggtcat tcctttttga tcttcacgac ctaaaaagac aatctgagtc attaagtgag
     1921 atacagcttg tggggtatag aactcacctg ctttttttcc tgactcactg gcaaattcac
     1981 caatcaagta ctcataagca tcacctaaag tatccccatc tactgcctca aagtcaattt
     2041 catttaatgt tttcatgaca ttagaaatgg tttgattttg tttttgagga gtagagccta
     2101 attttttaga gtagaggtca atatcttcaa ataagttttc aaagtcttca ccactttgtt
     2161 caatatcgcg aaaaccttga gctaatgatt ctaattggaa agtattgtga taaacatctt
     2221 ggatgagttt ttcaaaggtc agtgtcggtt cgataaagta acctaaatca ccagtaacaa
     2281 cagaaatgag gtcatctttt aggccttgat cttgataagc atcttcaaag attttttgag
     2341 catctgtaaa agtattaaag tgttcttcta aattatcaca aacagctatg agtaatttat
     2401 cagataagtg cttgtaaaag atgagtccaa gaagatagtt tttatagtca ttggcatcca
     2461 tttgaccacg taattggtcg gccgaatgcc atagtgcttg acggagtgaa gttgttttct
     2521 ctgccatgtg agtcctttct tatacgaata aacgattgag taatgtttgt ttgagtgctt
     2581 tgagttctgt tagtttttct tcagattgag caatttgttg gtctaaagtt tggaagaaat
     2641 taccaatggc ttcttgttct tttaatgtag gtatatttat ctttaattgt tcaacagatg
     2701 aataatttac atagatttga gtgccacctt gagcaatttt aatgatacca t
//
LOCUS       DAUGHY010000097         2709 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.097, whole genome shotgun sequence.
ACCESSION   DAUGHY010000097 DAUGHY010000000
VERSION     DAUGHY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.097"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(160..1437)
                     /locus_tag="VH752_001658"
     CDS             complement(160..1437)
                     /locus_tag="VH752_001658"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEQ8289987.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDRDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGRMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQAGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(1424..2599)
                     /locus_tag="VH752_001659"
     CDS             complement(1424..2599)
                     /locus_tag="VH752_001659"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEQ8289988.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APITEDIITLAASAADQILTDEDRSKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTANPRILALNKDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDFAAICFHIPFPKLALKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHRRIYHTNDKN"
BASE COUNT          826 a          493 c          530 g          860 t
ORIGIN      
        1 tatttataaa tacctcatca aactcagaat agagtagatg agtaaataga gtatgttatg
       61 gagactattt ctaagacttg tatgtataga aacaaagatt aaacacccat gtaaaccaat
      121 tgtttacatg ggtgtttgcc ttatattagt tgtttaaatc taagactgtc ttagctggga
      181 taagtaaaaa tgtgcttttt ctagatttat tggtttatca gctagtaaag cattaactaa
      241 aggagcaatt tcgtcttggg ttgcccccgc caaaagagct aatgatttcg catgtagttt
      301 catgtgacca gcttggatgc cagatgaggt cagagctttt agtgcagcaa agttttgagc
      361 taaccccaca gatgcaatca attgggctaa tgttttggca tcaggttgat taagaagatc
      421 atgtgcgata gaaacagtag ggttaagccc gatagatccc cccttactag caataggcat
      481 agggagggtc attcggccaa gcagaacttg tttatcttga tcaaaatgcc actgactaag
      541 tccttgatag ctaccatttc ttgaggcata agcatgggcc cctgcttcaa tagcacgcca
      601 gtcatttcct gtggctatta ccactgcatc gataccatta aaaatacctt tattatgagt
      661 agtagcccgg taaggatcta cttgagctag tcggctggct agctctattt tttgagctaa
      721 ctgtagggat tttgttttat cgcgatctaa aaggcgcacg ggaatctcac aactagtagt
      781 aacaagcgac tctgttgcgt aattagataa aatcgccatt agacaatgcc ccttagacag
      841 tcttgttaaa tcaggaacaa gagcttccat catagtattg accatatttg ctcccatagc
      901 ttcttgggta tcaacagtca gatagaaaat caaaaactct cctttttctt ccaaatgaat
      961 ggttctagcg cctccacctc ttttaacaat ggaaggataa gctttatttg ccaatgcaat
     1021 tagctttttt gttttatgaa ggatggcggc tttagcgtta tctatttggt cgatatcgta
     1081 aagaacaatt tgaccaatca tttgtcggtt tagggtttga gctttaaaac cacctgagcg
     1141 tttgattagt tttgctgcga aagatgctgc agcaacaaca gaaggttctt ccgtgacaaa
     1201 aggcatctga taagttgaac cgttgacaag aaaatcagga gctatgctaa agggcaaagc
     1261 caatcgtcct aagacatttt cagtcatttg attagctgtt tggataggca aaagaacgtc
     1321 tgttttgagt tgatttaagt tttcagcatt aagtagttta aatttttcga taagttggag
     1381 gcgttcttcg aatgttttct ttgaaaaacc gctccagtta agattagttt ttgtcattgg
     1441 tatgatagat ccttcgatgc tccttgatgg ctgttagagc aaattttcca gttaagtaaa
     1501 tgtcaaaatt ggcattacca ttatcatcta attggacttg ttcgtagaaa aggtcttcgt
     1561 atttagaaac ggaaagacgt gttcgttgat ccagtaaagc ttgtcgatta gtcattaaca
     1621 ttttatcgta cccagcaact aactgaccgg aataaaattc acttacggca ccggaaccat
     1681 agctaaaaaa accaatttta tctccagatt gtaatacttt actattttca agtaaagata
     1741 acaatcctag ataaagtgag ccagtataaa tatttccaat ttgtttgcta taagtaatag
     1801 aagcttgaaa ggcttctatt agtttttctc tgtgttcagg aggtactgtg ttatccataa
     1861 tgttatttag accttttagg gctaacttgg gaaatggaat atggaaacaa attgcagcaa
     1921 aatcagataa ctgaaggttt tctcttttct gataagcttg ccacgtggtt tctaagcaat
     1981 tgagatattg cttggtagag taaataccat caacataagg tgtgaaacta tagttaggcc
     2041 tccaaaagtc cataatatcc ctagtttgag ccacattatc tttatttaga gcaagaatac
     2101 gagggttagc agttaccaaa agagcaatac taccagagcc ttgagttgat tcgccaggag
     2161 atcctacacc gtatctagca atatcactag caataacaag gacacgagaa tctggcttag
     2221 aagccacatg cagtttagca tagtctagag cagcagttgc gctatagcag gcttgtttta
     2281 cttcaaagga acgtgcaaaa ggctggatac caactaagtg atgtacatag atagctgatg
     2341 cctttgactg atcagtactc gattcagttg ccaaaataac catatcaatc ttagatcgat
     2401 cttcgtcggt taaaatttgg tctgctgcag aagcagctaa agtaataatg tcttcagtga
     2461 ttggtgccac actaaaagac tcaatgagta gcccttgact aaattttgct ggatccactt
     2521 ggcgtgcaag cgctaaatct tccaatttca agacatattg actggttgca aaaccaatct
     2581 tatcaattcc aattgtcata tttgtccttc ttttagttat ttttatagtc ttacccattc
     2641 tatcacattt tactgcaaaa ttagactgat aaagttattt gttttatcct ttaatagtta
     2701 aaatgttca
//
LOCUS       DAUGHY010000098         2628 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.098, whole genome shotgun sequence.
ACCESSION   DAUGHY010000098 DAUGHY010000000
VERSION     DAUGHY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.098"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            14..685
                     /gene="sdaAB"
                     /locus_tag="VH752_001660"
     CDS             14..685
                     /gene="sdaAB"
                     /locus_tag="VH752_001660"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEQ8289989.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVYSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMDTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGVSIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
     gene            697..1569
                     /gene="sdaAA"
                     /locus_tag="VH752_001661"
     CDS             697..1569
                     /gene="sdaAA"
                     /locus_tag="VH752_001661"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEQ8289990.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKKQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIESQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
     gene            complement(1983..2597)
                     /locus_tag="VH752_001662"
     CDS             complement(1983..2597)
                     /locus_tag="VH752_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEQ8289991.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNADTKSYAKKS
                     ESKMVTIDKAPKNNHAITKEESKEKAKSIASEPIPTVENSVAPTVTEEAPVVQQEVTQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIARESNGNPN
                     AANASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
BASE COUNT          815 a          492 c          494 g          827 t
ORIGIN      
        1 aacggagttg cctatgaata cacaaaaatt tcaatcagtt tttgacatca ttgggccagt
       61 aatgattggt ccatcaagta gtcacacagc tggtgccgtc agaattggta aggtagtcta
      121 ttccatcttt ggagatattc ctgatgaagt gacgtttcac ctttataatt catttgctaa
      181 aacctacaga ggacatggta ctgataaggc tcttgttgca ggaattatgg gtatggatac
      241 tgataatcct gatattaaaa attctctaga aattgctcat caaaaaggga taaaaatcta
      301 ttgggatatc ttaaaagata gtaatgctcc ccatcccaat actgttaaaa tcagtgtcaa
      361 aaaagctgac aaaacactta gtgttactgg tgtttcaatt ggaggaggaa acattcaagt
      421 aacagaacta aatggttttt ctgtctcgtt atccatgaat actcctacta tagttactgt
      481 acacaaagat attcccggaa tgatcgcaaa ggtaactgat atcctatctt ccaataatat
      541 taatatcgcc acaatgaatg ttacacgtga atctgctggt gaaaaagcta ctatgattat
      601 agaagtcgat tctagagagt gccaagaagc agccaaccaa attgcaaaaa ttcctcatat
      661 ttataatgtc aacttctttg actagaaagg aaagctatgt tttatactat tgaagaactt
      721 gtcaaacaag ctgaccaaca atttaatgga aatattgctg aactcatgat tgccacggaa
      781 gtggagatgt ctggaagaaa tcgagaagat attatcaaaa tcatgtcacg caatttacaa
      841 gttatgaaag cagctgtaac agaaggacta acctcaacca aatctatcag tggtttgaca
      901 gggggcgatg cggtcaaaat ggataactac attaaaaaag ggaacagcct ttctgatact
      961 actattctta atgctgttag aaatgctata gctgtaaacg aattaaatgc taaaatggga
     1021 cttgtctgtg caacacccac tgcaggaagt gcaggatgct taccagcagt gctcgcaaca
     1081 gctattgaaa aacttgactt atcagaaaaa aaacaattag agtttttatt tactgcagga
     1141 gcttttggtc ttgttattgg aaataacgcc tctatttctg gtgcagaagg tggatgccaa
     1201 gcagaagttg gttctgcagc tgctatgagt gcagctgctc ttgttaaagc agctggtgga
     1261 acatctcatc aagctagtca agctattgcg tttgttatca aaaatttatt aggacttgtc
     1321 tgtgatcctg ttgccggcct agtagaagtg ccttgtgtca aacgcaatgc cctaggagca
     1381 agtttcgccc ttgtcgcagc tgatatggct ttagctgata ttgaatctca aattcctgtt
     1441 gacgaagtta ttgatgctat gtatcaagtc ggctctgcca tgccaactgc tttccgtgaa
     1501 acagcagaag gtggtctagc cgctactcca actggacgac gttacagcgt agaaatattt
     1561 ggcgaataaa tagatagtta gaaaaattct tagaaatatt tgaaagcgtt ttatttttat
     1621 gctatatatt cactaaaatg actaatagat agctatcaag ctatgttgtt tccccttaga
     1681 aacaacttct agtaaattgt taaaactcaa aagtgtagta gtgtaaaata ctgaaaattg
     1741 ctaatgacct tataaacatt taagagtttt tttcctgctt gttatcagta taactgcctt
     1801 aaatatcaga ggcacttaag tgctggtcag ttttcctaaa taatacgagc tatattccct
     1861 gataaggcaa tgttttgttt tgagactaat tcatcactac aaatcataaa gacaattaaa
     1921 taccaaaaac ctattgttag taaatactaa caataggttt tatttcttcc agccatgttt
     1981 atttagtaac cccaagctga taaaccttgt gcactatagg ctttcaaggc tgcattgact
     2041 tgatcttcaa ccgttgctgt agaaccccaa cctggcattg tctggaacaa ccctgatgcc
     2101 ccagaagcat tagctgcgtt aggatttcca ttagattcac gcgcaattat atgttcccaa
     2161 gttgattgtg gaacacctgt tgctgctgcc atctgtgccg ccgcttgact tcctacaata
     2221 ccagcagtat ttccattgga aagtaccaca ttgtttggat tataggctac tgaagatacc
     2281 tgctgaacag tttgagtcac ttcttgctga acaaccggtg cttcctctgt tactgtcgga
     2341 gctacagagt tttctactgt gggaataggc tccgaagcaa tgctctttgc tttttctttg
     2401 ctttcttctt tagtaatagc atgattattt tttggagcct tgtcgattgt taccatttta
     2461 ctttctgact tcttagcata agacttagta tcagcatttt tacttgagaa ggcaaaaatg
     2521 atggctaata ttgttagagc taacgctact aatccaaaat taaaataacg ttgttttaaa
     2581 ttttctttct taaacattca ataatacctc acttttctcc gttatcca
//
LOCUS       DAUGHY010000099         2625 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.099, whole genome shotgun sequence.
ACCESSION   DAUGHY010000099 DAUGHY010000000
VERSION     DAUGHY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.099"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            102..515
                     /gene="yaaA"
                     /locus_tag="VH752_001663"
     CDS             102..515
                     /gene="yaaA"
                     /locus_tag="VH752_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEQ8289992.1"
                     /translation="MIYKLFTEFITLQALLKELGIIQSGGAIKGFLAETTVLFNGEDE
                     KRRGKKIRVGDKISLPDQDLIITIVEPSQEEKEQFAEEMAEKTRVAALVKQMNQANKK
                     TSSKHNNRQSTTKKSLRATKKTKGKPTAPVRFPGI"
     gene            517..1623
                     /gene="recF"
                     /locus_tag="VH752_001664"
     CDS             517..1623
                     /gene="recF"
                     /locus_tag="VH752_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEQ8289993.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLASYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTNIYQQFLYQLEKNHQKDFFRKNTSVGPHRDELAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH"
     gene            complement(1678..2541)
                     /locus_tag="VH752_001665"
     CDS             complement(1678..2541)
                     /locus_tag="VH752_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEQ8289994.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWLFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTRPMQFVVGSLALLLLIVGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFEVLSVILPMAVGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLGETKTKKEMRWVVTGIICFI
                     VGAILLGVVKS"
BASE COUNT          923 a          504 c          436 g          762 t
ORIGIN      
        1 atttggacga gtatgatact ttataaatag ctcttattca gctattatta tttatttctg
       61 ttttatgcta taatgacttg aatgacaaaa tggagatgac aatgatatac aaattattta
      121 cagaatttat caccctacaa gcccttctta aagaattagg aattatccaa agtggtggcg
      181 ctatcaaggg atttctcgct gaaacaaccg ttctatttaa tggtgaagat gagaaacgtc
      241 gaggcaaaaa aatcagagta ggggataaga tttctctacc tgaccaagac ttaattatca
      301 ctattgttga gcctagtcaa gaagaaaaag aacaatttgc cgaagaaatg gctgaaaaga
      361 cacgtgtagc ggctctagtg aaacaaatga accaagctaa caaaaagact tcttctaaac
      421 ataacaatcg tcaatctact accaaaaaat cactaagagc aactaagaaa actaaaggta
      481 aaccaactgc acctgtccgc ttcccaggta tctagtatgt ggattaaaga gttagagtta
      541 aaacattatc gcaattatga ccacctgctt gcctcatttt cgtcgggttt aaatgtcttt
      601 atcggtaata atgctcaagg aaaaaccaat tttctcgagg caatctattt tctatcactt
      661 actagaagtc atcgcacgag ggctgataaa gaattgattc attttgatca ctcaactgtt
      721 tctcttactg gtaagattca gcgtattagt ggtactgttg atttagaaat caatctatca
      781 gataaaggac gtgtgacaaa gattaatgcg ctaaagcaag caaaattatc tgattatatt
      841 ggaacgatga tggttgtgct ttttgcacca gaagatttgc aactagttaa gggagcacct
      901 agccttcgtc gcaaatttat cgacattgac cttggtcaaa ttaagcctgt ttacttatct
      961 gagttatccc attataacca cgtgcttaaa cagcgtaata gctatttaaa aagtgcccaa
     1021 caaattgatg ccgcttttct agctgttcta gatgagcaat tagctagcta tggagcccgt
     1081 gtcatggaac atcgtattga ttttatcaat gcgttagaga aagaagcaaa tactcatcac
     1141 caagccatct caaatggttt agaaagtctc tctctctcct atcagtcgtc agttgtcttt
     1201 gacaaaaaaa ctaatattta ccaacaattt ttataccaac ttgaaaaaaa tcatcagaaa
     1261 gatttcttcc gtaaaaacac tagcgttggt ccacatcgcg atgaattagc cttttacatt
     1321 aacgggatga atgctaactt tgctagtcaa ggtcagcacc gcagccttat cctttcctta
     1381 aaaatggctg aagttagttt aatgaaggcc ttgactggag ataatcccat tcttttacta
     1441 gacgatgtca tgagcgagct agacaataca cgacaaacaa agttacttga aacagtaatt
     1501 aaagaaaatg tccaaacctt tatcacaaca acaagtttgg atcacctgtc acagttgcca
     1561 gaaggtatcc gtatttttca cgtgacaaaa ggaaccgttc aaattgattc tgatattcac
     1621 taataacatc aaaaaagggt tcagttaaac tgagcctttt ttgatattat gtggcgatta
     1681 agatttgacc acacctaata aaatagcacc tacgataaaa caaataattc ctgtgacaac
     1741 ccaacgcatt tcttttttgg ttttggtttc gcctaggaat aaaatgcctc cgacaatcga
     1801 aatgatagca cctaattgtg agaaactaaa ggcaatagca agtcctgctt ttgatgcagc
     1861 taaaagcata aaaatattcc caatgcccca aagtaatcca acaacgctat ttttaatcac
     1921 atactggtca atggaaattt taaatgacat gaaagttata gctcctaaaa ccatacctac
     1981 tgccatgggt aaaatgactg acaaaacttc aaatttcatg atgttattaa ataatacagc
     2041 atacatcacg taaccaattg ttgaataagt gagcgcccta aatccttttg aaaaattatg
     2101 aaggtgattg acttgtgcat tagcatcatc ttgtttacta gaaaagtaaa aaccaacaat
     2161 cagaagtaat agtgctagac ttcctactac aaattgcata ggtcttgtcc attcatgaaa
     2221 aacgaggaca ccaatcaaac ttcccagaac aagttgagag ccacttgaca aaggattggc
     2281 aacggagact cccatgtatt gcatggcatg aaattgacca gtttgaccaa ttgaccagat
     2341 aaaaccaccc aatattccaa aaagccaaag ttgcagtgtc atctcaggtc taacaattaa
     2401 ccaaacagct aatgaaaata ataaagcccc aaaagtcatt cctagagttt gttgcgaagg
     2461 ttttcctcca attttgttac taacaaatcc aatgcttccc catgtaaaca tgggaataag
     2521 tgcataaaaa ataccttcca aaatgttatc tcctttatag taaatcttta aagattataa
     2581 taattctatc ataaaatggt ttcatgtaaa gactaattat agctt
//
LOCUS       DAUGHY010000100         2414 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.100, whole genome shotgun sequence.
ACCESSION   DAUGHY010000100 DAUGHY010000000
VERSION     DAUGHY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.100"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            470..1762
                     /gene="purB"
                     /locus_tag="VH752_001666"
     CDS             470..1762
                     /gene="purB"
                     /locus_tag="VH752_001666"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEQ8289995.1"
                     /translation="MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEQYVCYKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDIKVTSCLTQEEIDELFNPIYYTKRVDDIFKRLGI"
     gene            1894..>2414
                     /gene="rgg4"
                     /locus_tag="VH752_001667"
     CDS             1894..>2414
                     /gene="rgg4"
                     /locus_tag="VH752_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Rgg4/ComR"
                     /protein_id="HEQ8289996.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKIIIDCLQATLDTLLSENTNFGIDLLQEYFNQIKTKVRFRQND
                     LILLELYLAYLDI"
BASE COUNT          776 a          409 c          539 g          690 t
ORIGIN      
        1 gtgcagaatc ttggtatagt gagcgtcaaa tgcaggatgt aattaaaaaa attaatgaaa
       61 aatacactat attggaagat agtggaaggg agtcggatta atcaaattaa atttggtaga
      121 gagtttagga aatctaactt actcattcta aatgctaagg aaattaagtt aaaaaataga
      181 aagaaacaaa ggtagtcgta ttcgctaaac tgaatacagg ctacggactt ccttaactca
      241 cagaatttta agaaatacaa gaaaggagtg agggtgtttc cgagtatttg aactcgggct
      301 aacttatctc ctcaatacaa atcaaataat agaaaggaaa tgggcactag tttcactaaa
      361 ctgaacccga gcggaaagct cggaatttag ataaaccgcc taggacgcaa gcgtccattg
      421 ttggtttcct aaaattcagt cgctttctgg tcgcccttgg tatcttaaaa tgatcgaacg
      481 ttattcacgc cctgagatgg cggcaatttg gacagaggaa aataaatacc gtgcttggtt
      541 ggaggttgag attttggctg acgaggcatg ggctgagttg ggtgagattc ctaaggagga
      601 tgtggctaag attcgtgaga aggcggattt tgacattgac cgcattcttg agattgagca
      661 ggacacacgt cacgatgtgg tggctttcac tcgtgcggtt tctgaaacgc ttggtgagga
      721 gcgcaagtgg gtgcactacg gtttgacgtc aactgacgtg gtggacactg cctacggtta
      781 cctctacaag caagctaacg acattatccg tcgcgatctt gagaatttca ccaatatcgt
      841 ggcagacaag gcgcgtgagc acaaaatgac catcatgatg ggtcgcaccc acggtgttca
      901 cgccgagcca acgactttcg gtcttaagtt ggcgacttgg tacagcgaga tgaaacgtaa
      961 tattgagcgt tttgaacatg ctgccgcagg tgtggaagct ggtaagattt caggtgccgt
     1021 tggtaacttt gctaatatcc ctccatttgt ggaacaatat gtctgttaca aattaggcat
     1081 tcgtccacaa gagatttcaa cacaagttct tccacgtgac ctccacgcag aatattttgc
     1141 agtgcttgca agtattgcaa cttctatcga acgtatggcg acagagattc gaggtctgca
     1201 aaagtcagaa caacgtgaag ttgaagaatt ctttgccaaa ggtcagaaag gtagctctgc
     1261 tatgcctcac aaacgcaacc caatcggttc agagaacatg acagggctag cgcgcgtgat
     1321 tcgtggtcac atggtgacag cttatgagaa cgtgtcactt tggcatgagc gtgatatttc
     1381 acactcatca gctgagcgta tcatcacacc tgacacaact atcttgattg actacatgct
     1441 caaccgcttt ggcaatatcg ttaagaactt gactgtcttc ccggaaaata tgatgcgcaa
     1501 tatggaatca acttttggtt tgatttatag tcaacgtgtt atgctcaaat tgattgaaaa
     1561 aggaatgaca cgagaagaag cttatgactt agttcaacct aagacagctt attcctggga
     1621 caatcaagtg gatttcaaac cacttttaga agaagacatc aaagttacct cttgtcttac
     1681 acaagaagaa attgatgaac tatttaatcc gatttattac acaaaacgtg ttgatgatat
     1741 ttttaagcgt ttagggattt aatataaaaa aaagaaggtg attcccttct tttttgttta
     1801 aaaaaacgaa tattttcttt aaaaatatta aaaaggttag tttttgatta gacgctgtga
     1861 tataataaag aaaacagttg ttggagagaa accatgttag aacattttgg tggaaaagta
     1921 aaagtgttaa gacttgaaaa gaggattagt cgcgaggact tgtgtgggga tgagtctgaa
     1981 ctttctgttc gtcaattagc acggatagaa ctaggtcaat ccataccaag tttaagtaag
     2041 gttattttta ttgcaaaagc cttaaacgtt agtgtcggtt acttaactga tggtgctgat
     2101 ttagaactac ctaagcgtta caaagaatta aaatacctta tcttaaggac accaacttac
     2161 atggatgatg gaaaattaca agtacgagaa gagcagtttg atgaaatttt tgaggattat
     2221 tatgataaat taccagagga agagaaaata atcattgatt gtttacaggc aactttagat
     2281 actttattga gtgagaatac taactttggc attgacttac ttcaagaata ttttaatcaa
     2341 ataaagacta aggtacgctt taggcaaaat gatttaatac ttctagaatt atatttagct
     2401 tatcttgata ttga
//
LOCUS       DAUGHY010000101         2204 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.101, whole genome shotgun sequence.
ACCESSION   DAUGHY010000101 DAUGHY010000000
VERSION     DAUGHY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.101"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..1113
                     /gene="hasA"
                     /locus_tag="VH752_001668"
     CDS             <1..1113
                     /gene="hasA"
                     /locus_tag="VH752_001668"
                     /EC_number="2.4.1.212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronan synthase HasA"
                     /protein_id="HEQ8289997.1"
                     /translation="LSFLYEPFKGKPHDYKVAAVIPSYNEDAESLLETLKSVLAQTYP
                     LSEIYIVDDGSSNTDAIQLIEEYVNREVDICRNVIVHRSLVNKGKRHAQAWAFERSDA
                     DVFLTVDSDTYIYPNALEELLKSFNDETVYAATGHLNARNRQTNLLTRLTDIRYDNAF
                     GVERAAQSLTGNILVCSGPLSIYRREVIIPNLERYKNQTFLGLPVSIGDDRCLTNYAI
                     DLGRTVYQSTARCDTDVPFQLKSYLKQQNRWNKSFFRESIISVKKILSNPIVALWTIF
                     EVVMFMMLIVAIGNLLFNQAIQLDLIKLFAFLSIIFIVALCRNVHYMIKHPASFLLSP
                     LYGILHLFVLQPLKLYSLCTIKNTEWGTRKKVTIFK"
     gene            1149..>2204
                     /gene="hasB"
                     /locus_tag="VH752_001669"
     CDS             1149..>2204
                     /gene="hasB"
                     /locus_tag="VH752_001669"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase HasB"
                     /protein_id="HEQ8289998.1"
                     /translation="MKIAVAGSGYVGLSLGVLLSLQNEVTIVDILPSKVDKINNGLSP
                     IQDEYIEYYLKSKQLSIKATLDSKAAYKEAELVIIATPTNYNSRINYFDTQHVETVIK
                     EVLSVNSHATLIIKSTIPIGFITEMRQKFQTDRIIFSPEFLRESKALYDNLYPSRIIV
                     SCEENDSPKVKADAEKFALLLKSAAKKNNVPVLIMGASEAEAVKLFANTYLALRVAYF
                     NELDTYAESRKLSSHMIIQGISYDDRIGMHYNNPSFGYGGYCLPKDTKQLLANYNNIP
                     QTLIEAIVSSNNVRKSYIAKQIINVLEERESPVKVVGVYRLIMKSNSDNFRESAIKDV
                     IDILKSKDIKIIIYEPML"
BASE COUNT          750 a          353 c          374 g          727 t
ORIGIN      
        1 ttatctttcc tttatgagcc atttaaagga aagccacatg actataaagt tgctgctgta
       61 attccttctt ataatgaaga tgccgagtca ttattagaaa ctcttaaaag tgtgttagca
      121 cagacctatc cgttatcaga aatttatatt gttgatgatg ggagttcaaa cacagatgca
      181 atacaattaa ttgaagagta tgtaaataga gaagtggata tttgtcgaaa cgttatcgtt
      241 caccgttccc ttgtcaataa aggaaaacgc catgctcaag cgtgggcatt tgaaagatct
      301 gacgctgacg tttttttaac cgtagattca gatacttata tctatccaaa tgccttagaa
      361 gaactcctaa aaagcttcaa tgatgagaca gtttatgctg caacaggaca tttgaatgct
      421 agaaacagac aaactaatct attaacgcga cttacagata tccgttacga taatgccttt
      481 ggggtggagc gtgctgctca atcattaaca ggtaatattt tagtttgctc aggaccattg
      541 agtatttatc gacgtgaagt gattattcct aacttagagc gctataaaaa tcaaacattc
      601 ctaggtttac ctgttagcat tggggatgat cgatgtttaa caaattatgc tattgattta
      661 ggacgcactg tctaccaatc aacagctaga tgtgatactg atgtaccttt ccaattaaaa
      721 agttatttaa agcaacaaaa tcgatggaat aaatcttttt ttagagaatc tattatttct
      781 gttaaaaaaa ttctttctaa tcccatcgtt gccttatgga ctattttcga agtcgttatg
      841 tttatgatgt tgattgtcgc aattgggaat cttttgttta atcaagctat tcaattagac
      901 cttattaaac tttttgcctt tttatccatc atctttatcg ttgctttatg tcgtaatgtt
      961 cattatatga tcaaacatcc tgctagtttt ttgttatctc ctctgtatgg aatattacac
     1021 ttgtttgtct tacagcccct aaaactttat tctttatgca ccattaaaaa tacggaatgg
     1081 ggaacacgta aaaaggtcac tatttttaaa taatatgtgc atcgagtagt tagagaagga
     1141 gtaattttat gaaaatagca gttgctggat caggatatgt tggattatca ctaggagttc
     1201 ttttatcact tcaaaacgaa gtcactattg ttgatattct tccctctaaa gttgataaga
     1261 ttaataatgg cttatcacca attcaagatg aatatattga atattactta aaaagtaagc
     1321 aattatctat taaagcaact ttagatagca aagcagctta taaagaagcg gaactggtca
     1381 ttattgccac acctacaaat tacaacagta gaattaatta ttttgataca cagcatgttg
     1441 aaacagttat caaagaggta ctaagcgtta atagccatgc aactcttatc atcaaatcaa
     1501 caattccaat aggtttcatt actgaaatga gacagaaatt ccaaactgat cgtattatct
     1561 tcagccctga atttttaaga gaatctaaag ctttatatga caacttatat ccaagccgaa
     1621 ttattgtttc ttgtgaagaa aacgattctc caaaagtaaa ggcagacgca gaaaaatttg
     1681 cacttttatt aaagtctgca gctaaaaaaa ataatgtacc agtacttatt atgggagctt
     1741 cagaagctga agcagtaaaa ctatttgcca atacttattt agcgttaagg gtagcttatt
     1801 ttaatgagtt agacacttac gcagaatcga gaaaattaag tagtcacatg attattcaag
     1861 gaatttctta tgatgatcga ataggaatgc attataataa cccatcattt ggttatggag
     1921 gttattgtct acctaaagat acgaagcaat tattggcaaa ttacaataat attcctcaaa
     1981 cgctaattga agctatcgtt tcatcaaata atgtgcgcaa gtcctatatt gctaagcaaa
     2041 ttatcaacgt cttagaagag cgggagtccc cagtaaaagt agtcggggtt taccgtttaa
     2101 ttatgaaaag taactcagat aattttagag aaagtgctat caaagatgtt attgacattc
     2161 ttaaaagtaa agacattaag ataattattt atgagccaat gtta
//
LOCUS       DAUGHY010000102         2079 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.102, whole genome shotgun sequence.
ACCESSION   DAUGHY010000102 DAUGHY010000000
VERSION     DAUGHY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.102"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(16..626)
                     /locus_tag="VH752_001670"
                     /pseudo
     CDS             complement(16..626)
                     /locus_tag="VH752_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            945..1172
                     /locus_tag="VH752_001671"
     CDS             945..1172
                     /locus_tag="VH752_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEQ8289999.1"
                     /translation="MEVKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAI
                     GFPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            1185..1385
                     /locus_tag="VH752_001672"
     CDS             1185..1385
                     /locus_tag="VH752_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HEQ8290000.1"
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLLHP
                     VLAGGCVVGFTVGGAYLGYTAN"
     gene            1679..1873
                     /locus_tag="VH752_001673"
     CDS             1679..1873
                     /locus_tag="VH752_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEQ8290001.1"
                     /translation="MEVLKNGLAIINGGGKDGANIFLTGMAGAAQGVTVCAQTGVFIP
                     WQGYILCGAAGAATNIIWPH"
BASE COUNT          651 a          309 c          435 g          684 t
ORIGIN      
        1 tgtcttgtaa gtattttagt cttcgaaaat acttacaaga catcgttcta aggcttcttc
       61 atttgggaaa accactctct tcttgttttg tcgcttaatt tctttattga gtgattcaat
      121 caagtttgta gagtaaatgc tagaccaaat gtgatgtggg aattgataga aagtaaggag
      181 attttccgtc gtttcaagtg atgccatgac ctttttataa cgaggcttcc actcagaaac
      241 aagtggtgac catacatgcg ctcaataatg tcactgattt cgcgtgtcgt aacgccagtc
      301 tgatagagtt tgatcaccat ctcttccaag tggtcatctc gtctagcata agatggtaat
      361 aaaactggtg aaaactcgcc gtttcgatct cttgggatga ttaaattgac taagccatac
      421 tttgtttcaa actgacgaga ataagtccca ttacggctat tacctgtgtt gtaaccttct
      481 ttttcataag gttcataacc aagaaaagct gatagttcaa cttgcaagag gtcattcata
      541 gctatttcta aagaggagcg aaagaattca tcaatatctt gtttttgtgc taggaagtta
      601 agtaattctg tggtaaactg agtcatggga atgaatctct ttctagtgaa gtgtcgtaac
      661 tctactatac aagatttatt cctttttgtg tgtttacaca agatatttta cactaccatc
      721 aaaaatctta aaaggtattt ataaaagcat gaatggtatt atttaaactt cttaattaat
      781 taactattgt cacagcgtaa gtgtagagag gataaaaaga catttcatga tgaaaaacag
      841 ccttttaaga ttttgaaacc atgatactgt tctttatgat attctgtact agctcgaggt
      901 tgcaacgaga caaatttaag aattatgaaa agaagttatt cgatatggag gtaaagaaac
      961 tggaaacatt tcaccaaatg actatagaaa agcttgccaa ggttgagggt ggtaagaata
     1021 attggcaggc aaatgtcagt ggggttatcg ctgcaggtag tgctggagct gctataggtt
     1081 ttccagtttg cggtgtagcc tgtggttata ttggagctaa aacagctatt actctttggg
     1141 ctggtgtaac aggagctaca ggcggattct agaggaaaga aaggatgaca acaatgaagg
     1201 aattaacaat taatgacatg gctagtataa gtggaggaaa cgctccagga gatgctgtaa
     1261 ttggtggtct tggaggctta gcttctggat tgaaattttg taaactgcta catccagtat
     1321 tggcaggtgg atgtgttgta ggatttacag ttggtggagc atatttggga tatacagcaa
     1381 attaaatgat ctatacgatt tttattatta tgaatgcgtc tataatgttt ttaatcattg
     1441 ttgatgccat tcataaaaaa atgttgataa atctatacca tatttagtgt ttttaatttt
     1501 caatcaatta tccctttcag aaaaatcggt aaacatgtta tctatcgtag ctttggtagc
     1561 gtcattattt gtttttatcg ataaaagaaa atagtgatta tgccaatgaa tttagatatc
     1621 actcttagct ttttaaaata caaaagagtg aaattaaaat gaaaaagact tcaaaaatat
     1681 ggaagtgcta aaaaatggtc tggctatcat aaatggaggc ggaaaagacg gagcaaatat
     1741 ctttttgaca gggatggcag gtgctgcgca gggagtgaca gtttgtgctc agacaggcgt
     1801 atttatccct tggcaaggtt atattttgtg tggagcagct ggtgctgcta caaatattat
     1861 ctggcctcac taaatatata aatgactatc tatcactgat gtataagtag tagaggagtg
     1921 gtatgaaaac tttagatatt atttttaatc tatctgatag aaagatgata tttctagaaa
     1981 atgttgaaaa gctgggaggt tacattgtcc cttttaccct tatcatcttg agtgtagcct
     2041 acaactttag gcacccttat cctatttggg ctgctgtac
//
LOCUS       DAUGHY010000103         1977 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.103, whole genome shotgun sequence.
ACCESSION   DAUGHY010000103 DAUGHY010000000
VERSION     DAUGHY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.103"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            646..1257
                     /gene="rpsD"
                     /locus_tag="VH752_001674"
     CDS             646..1257
                     /gene="rpsD"
                     /locus_tag="VH752_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEQ8290002.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVDPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1411..1944)
                     /locus_tag="VH752_001675"
     CDS             complement(1411..1944)
                     /locus_tag="VH752_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ8290003.1"
                     /translation="MVNRKENTKQAILKAMVMLLKTESFDDITTVKLSKRAGISRSSF
                     YTHYKDKYEMIDYYQQTFFHKLEYIFEKKYQNKEQAFLEVFEFLQREQLLSSLLSANG
                     TKEIQAFIINKVRLLITTDLQDKFRTEELSQTEKEYRSIYLAHAFFGVCQSWIAKGKK
                     ESPQEMTQFVLKMLTST"
BASE COUNT          630 a          344 c          346 g          657 t
ORIGIN      
        1 ttttgaaaaa attcctataa aagataatat ttttaggtta gattgaagga aaaaagaggt
       61 cttcaccatg gttaagcaaa tgatcttagt actaaggtta aaagattatc gtgaaataaa
      121 gttttgacac aagtgtaatt aatagaggaa cttgaagtat ccggtaaaac tatcttgaag
      181 tgaaaaaacg agtagacctc ccctgataaa tacaatatac tagccatttc agagcaatta
      241 agagtttcca gtgaagtgct tttgatagag agctaagaaa aaaacagtca aggtttaaga
      301 actgcaaatt agcatttaaa ttattttttg ttaatgacag ttgtctttat taccataact
      361 ataacttctt ataatatagt cttcttaatt catcagtggt ggttcgtccc atatctgacc
      421 ttaaaatatt aaactgatca tcagatgata gatagactga ctttacaaaa caactttctt
      481 atggtaaact gttctttgtg cgaaataaca gcagaaaaat atgaagctcg tcaacaggtg
      541 gttatctatt caagtaacct ttgctgttta ggcgaaaacc ccctgcacga atcaggattt
      601 tttaaaccgt tatttcctca aaaaatttag aggaggacat gtaatatgtc acgttatact
      661 ggtccatcat ggaaacaatc acgtcgcctt ggtttatcac ttacaggcac aggtaaagaa
      721 ttggcacgtc gtaactacgt tcctggtcaa cacggtccaa acaaccgtag caaactctca
      781 gaatatggtt tgcagttagc tgagaaacaa aaacttcgtt tctcatacgg tttaggtgaa
      841 aaacaattcc gtaacttgtt cgttcaagct acaaaaatca aagaaggaac tcttggtttc
      901 aactttatgg ttcttttaga acgtcgtctt gacaacgttg tttaccgtct aggtttagca
      961 actactcgtc gtcaagcacg tcaattcgtt aaccacggtc acatccttgt tgacggtaaa
     1021 cgcgttgata tcccttcata ccgtgttgat ccaggtcaag tgatttcagt tcgcgaaaaa
     1081 tcaatgaaag tacctgcaat ccttgaagct gttgaagcta ctcttggacg tccagctttc
     1141 gtatcatttg atgctgaaaa acttgaaggt tcattgactc gccttccaga acgcgatgaa
     1201 attaacccag aaatcaatga agcacttgtc gttgaattct ataacaaaat gctttaattt
     1261 taagattaaa cttacaaaaa gcctatttaa taggcttttt ttgtatctca taacaaatct
     1321 attctgatcc cttcatttta ctatggcttc taaaaaagtt atcactaagg ctgatgactt
     1381 gtaaaatgat actttcgtaa gggttactat ttaggtactc gtaagcattt tgagaacgaa
     1441 ttgtgtcatt tcttgaggtg attctttttt acctttagca atccaacttt gacagacacc
     1501 aaaaaaagca tgggctaagt agatacttcg gtattctttt tccgtttgag ataactcttc
     1561 ggtgcggaac ttgtcctgta aatcagtagt gatgagtagg cgaactttat tgatgataaa
     1621 ggcctgtatt tctttggtac cgttagcaga caaaagggaa gagagaagct gttctctttg
     1681 gagaaattca aatacttcaa ggaaggcttg ttctttattt tgatattttt tctcaaaaat
     1741 atattctaat ttgtgaaaaa aggtttgctg ataatagtca atcatttcat atttatcttt
     1801 atagtgtgtg taaaaactag agcgactaat tccagctctt ttagataatt taactgtggt
     1861 aatatcgtca aaactttcgg tttttagtaa cataaccatt gcttttaaaa ttgcttgctt
     1921 cgtattttct ttacggttga ccatctacca tctctccttt tgaacacttt tagacat
//
LOCUS       DAUGHY010000104         1893 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.104, whole genome shotgun sequence.
ACCESSION   DAUGHY010000104 DAUGHY010000000
VERSION     DAUGHY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.104"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            87..728
                     /locus_tag="VH752_001676"
     CDS             87..728
                     /locus_tag="VH752_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEQ8290004.1"
                     /translation="MKDLLKQYQAKPLGEEKRYAVFLPLILVNDDWHVLYEVRSQHIS
                     QPGEVSFPGGQVENQETLQEAAIRETVEELTVDASQIQLWGEIDYLVQSSRTIHCFVG
                     QLVVDDWESIQPNEEVDKVFTVPLRQLLVTDPVYYHLEATPIETTDFPFDRIRNGKYY
                     QFSQEYRSIPFYENLEETIWGMTAQFTKCLTDILNDESIAKKNPLDIEKSKGR"
     gene            complement(775..1617)
                     /locus_tag="VH752_001677"
     CDS             complement(775..1617)
                     /locus_tag="VH752_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEQ8290005.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERMISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVIVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKVDWLWLVLTFVISGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMAVVLFALSIWLGLWMSQLE
                     EKYK"
BASE COUNT          687 a          328 c          359 g          519 t
ORIGIN      
        1 actaactagg agtaataagt tgaccaaaaa gccaggattg gttatgatga agataagatt
       61 aacagaaaga gaaattaggg aaggctatga aggatttatt aaaacagtat caggcaaaac
      121 cactaggaga ggagaagcgg tacgccgtat ttttacctct tattcttgtc aatgatgact
      181 ggcatgtctt atatgaagtc agaagtcagc atatttcaca accgggggaa gtttctttcc
      241 cagggggaca agtagaaaat caggagacac ttcaagaagc tgctatccga gaaacagttg
      301 aggaattgac tgttgatgcg tcccagatcc agctatgggg tgaaattgat tatttagtac
      361 agtcttctag aactattcat tgtttcgttg gtcaattagt cgttgatgac tgggaaagca
      421 tacagcctaa cgaagaagtt gacaaggtct ttacagtacc tttacgtcaa ctacttgtca
      481 ctgatcctgt ctattatcat ttagaagcca ctcctattga gacaacggat tttccttttg
      541 accgtattcg taatgggaaa tactaccaat ttagtcaaga gtaccgtagt attccgtttt
      601 acgaaaatct agaagaaacc atatggggaa tgactgccca gtttactaag tgtttaaccg
      661 atattctaaa tgatgaatca attgcaaaga aaaacccctt agatattgaa aaatctaagg
      721 ggagatagag cactagtttg ttggtgtcaa ctttttgtca acaacctgta aaggttattt
      781 gtatttttct tctaattgac tcatccaaag tccgagccaa atactaagag caaataacac
      841 aacggccata agccaggtta agatacctgc atgactgtag gaaatggatt cgggactact
      901 actatttgga ataagaatta atagcgtgct ggataataca ataccaatga taaagtggta
      961 aacttttgaa tgatggtgct gtaaagcata atccatactt ttagaaaaag ccaagatggc
     1021 taggactcct ccgatagcaa taggtaagaa agtccctaat aaatccaaag atttgaatcc
     1081 tattaacata ggaccataga gtcctaagat aagtaagaga tttgacgggc ttagtccagg
     1141 gaccagtact cctaaagcaa ttaaggcacc tgctaaaata aaggttaaaa agttagctgg
     1201 taaggtaccg attaagtcat ttaagaagta aagacctagt cctgaaataa caaaggtaag
     1261 gactagccat agccagtcaa ctttgtctct ttgagactgc ttggttgact ccttaatcag
     1321 actaggaata gtgccgacaa tagcgccagc aaatccccat aagacgataa cttgatagtg
     1381 cttaagcaaa aactcaacgg ggaaagaaaa gagagcaata cctaaaatac caccaattcc
     1441 tactggtaag aaaaataaga cattttcaat aaagttatcc ctcatatgag ctaaaaagga
     1501 aatcattcgc tcatagattc ctaaaatggc tgcgagaaca ccaccggata caccaggtaa
     1561 aataaaacct agtgcaataa tcatgccttt aaaaactctt gaaataaatg aaaccattgt
     1621 tgcctcctga aaagaatgat tttattgtat cataaattta aagaattgct gacacaaagc
     1681 tgcatatccc tactaaaata attgttccta ataaataata cattcaattt tgaagatatt
     1741 ttccttaaac accatgtctt tgtgatcaag tcaagtttga ttgtcaaagt tgtaaataaa
     1801 ttttacaaag actgtaaacc ttacgataat tatcaaaaag ataaacgaaa aaaagcttag
     1861 gtcaaagact tttgatagta aacttattga aga
//
LOCUS       DAUGHY010000105         1775 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.105, whole genome shotgun sequence.
ACCESSION   DAUGHY010000105 DAUGHY010000000
VERSION     DAUGHY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.105"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            283..399
                     /locus_tag="VH752_001678"
                     /pseudo
     CDS             283..399
                     /locus_tag="VH752_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(585..>1775)
                     /locus_tag="VH752_001679"
     CDS             complement(585..>1775)
                     /locus_tag="VH752_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEQ8290006.1"
                     /translation="QELGKGITAINKAYRPGEGVKARKEAAKADLEKEAAKVKALITN
                     DPTLTKADKAKQTGAVAKALKAAIAAVDKATTAEGINQELGKGITAINKAYRPGEGVK
                     ARKEAAKADLEREAAKVREAIANDPTLTKADKAKQTEAVAKALKAAIAAVDKATTAEG
                     INQELGKGITAINKAYRPGEGVKARKEAAKANLEKVAKETKALISGDRYLSETEKAAQ
                     KQAVEQALAKALGQVEAAKTVEAVKLAENLGTVAIRSAYVAGLAKDTDQATAALNEAK
                     QAAIEALKQAAAETLAKITTDAKLTEAQKAEQSENVSLALKTAIATVRSAQSIASVKE
                     AKDKGITAIRAAYVPNKAVAKSSSANHLPKSGDANSIVLVGLGVMSLLLGMVLYSKKK
                     ESKD"
BASE COUNT          479 a          370 c          327 g          599 t
ORIGIN      
        1 caataactat gtgtaaacaa tcaagtagaa atgtctttat taagcgaatc aatcaaggtt
       61 gtagagtaaa tgctggacta gatttcatgt agaaattagt aaaagttagg agattttttg
      121 gtcgtttcaa acgataacat catcttctta taacaagaac ttcactcaga gataaactat
      181 tctcatactt tagtagcctc ttttgagctt gtagcacgaa aattcgctcg aactgattta
      241 aaataggcat tttatctgct cgcttgatcc ctactgcgca accaagaaac cttttattca
      301 ctagaacaat taacccttaa aaccaaggag gaagtccact ggtggaacta ccatcacctt
      361 cacagtaatc ttaattatca aacacctata actaaatgag tcatcgtttg aacaaaaaac
      421 gccttataaa ttctgtacag aaaactgttg ccttttcaat tgaactttga aatgttttca
      481 atacactccg ttcttgagcc cttacgatcc aaaaaaccaa ggtgatttca aaatcacctt
      541 ggtttgaaaa tatagtagaa agcaatttgg ataaagccag gttcttagtc tttactttct
      601 tttttcttgc tataaagcac catacctaaa agaagagaca taactcctaa gccaacaaga
      661 acaattgagt ttgcatcacc tgattttgga agatggttcg ctgacgatga ttttgcgact
      721 gccttattag gcacataggc tgcacggata gcagtaatac ctttatcttt tgcttctttc
      781 acagacgcaa tagattgtgc tgaacgaaca gtcgcaatag ccgtcttaag cgctaatgat
      841 acattttctg attgttcagc tttttgagct tcagtcaatt tagcatcagt tgtaatctta
      901 gcaagtgttt ctgccgcagc ttgtttaaga gcttcaatag cagcttgttt cgcttcgtta
      961 agagcagctg ttgcttgatc agtatcttta gctaaaccag caacatatgc tgaacggata
     1021 gctacagtac caaggttttc tgccaactta acagcttcaa ctgtcttagc agcctcaact
     1081 tgaccaagtg ctttcgcaag agcttgctca acagcttgtt tttgggctgc tttttcagtt
     1141 tcgctcaagt aacggtctcc tgaaataaga gctttagttt ctttagctac tttttcaaga
     1201 ttagctttag cagcttcttt acgtgcttta acaccttcac ctggacggta agctttgttg
     1261 atagctgtga tgcctttacc aagttcttgg ttaatgcctt cagctgttgt cgctttatca
     1321 accgctgcga tagcagcttt aagagcttta gcaacagctt ctgtttgttt agctttatca
     1381 gcttttgtta aggttgggtc gttagcgata gcttcacgaa ccttagcagc ttctctttca
     1441 agatcagcct tagcggcttc tttacgtgct ttaacacctt cacctggacg gtaagctttg
     1501 ttgatagctg tgatgccttt accaagttct tggttaatgc cttcagctgt tgtcgcttta
     1561 tcaaccgctg cgatagcagc tttaagggct ttagcaacag ctcctgtttg tttagcttta
     1621 tcagcttttg ttaaggttgg gtcgttagta ataagagctt tcactttagc agcttctttt
     1681 tcaagatcag ctttagcggc ttctttacgt gctttaacac cttcacctgg acggtaagct
     1741 ttattgatag ctgtgatgcc tttaccaagt tcttg
//
LOCUS       DAUGHY010000106         1761 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.106, whole genome shotgun sequence.
ACCESSION   DAUGHY010000106 DAUGHY010000000
VERSION     DAUGHY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.106"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(26..589)
                     /gene="thiT"
                     /locus_tag="VH752_001680"
     CDS             complement(26..589)
                     /gene="thiT"
                     /locus_tag="VH752_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEQ8290007.1"
                     /translation="MSPNTNVKYLIEAAIFAALAMTLSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSDSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     regulatory      complement(634..730)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(858..1706)
                     /locus_tag="VH752_001681"
     CDS             complement(858..1706)
                     /locus_tag="VH752_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEQ8290008.1"
                     /translation="MRRKIKPIVVLVFFILLAMVLIIGKRQANHAKQKEVEDAKSHIP
                     IATSNPSKAKTSTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFKTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARAFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDAV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
BASE COUNT          579 a          325 c          322 g          535 t
ORIGIN      
        1 aatgtaagat tcaaatgact aaaaattagt ctttgggatc aaataattga ggatgaattg
       61 gtaaagcaat cattaacgct aaacatgtta ttaaaaaagt taggacacca gctgtaccat
      121 taacgctaaa agaataccag acagcggaca tgcctttagg ggcgtaactg ccccagaaaa
      181 tgattcctgc aaggaagtgc cacaagtaac gtactgtaac ggctagtaaa ctagccatta
      241 ttgcaagaaa aacggcaaaa aaggtttgtt gtcttcttaa agttttaatc aaactatcag
      301 agaaactacc ggctaatccc atagaggtaa aggctaagat atattcaata aagacttggg
      361 acatgctgag atagtaaact tttccaagta caaagtgaag tagcccccaa atgagacccg
      421 ccaacatgcc gtactttaac ccacggcgca aagagaaaat aaccagtgca atagcaccat
      481 aagaaggact aaaccagcca gcaaaatctg gaataaatga aagggtcatc gccaatgcag
      541 caaaaatagc agcttcgata aggtatttga cattggtgtt tggtgacata aaaaactcct
      601 tttgtgcaca aaaagagtat cgctttccaa tcattgatta ttggtcacaa tccctacgct
      661 tgtactaaca agatcaggtc ctcaggattt cgcaattgcg atctcagcca aagcactcct
      721 ttgtgaaata ttatagtgct ttcattatat gatgagataa tctccttgtc aaggataagg
      781 ggttttaaat tattgaccta gagtttttct tggtaagatg taaactataa aaaaagatta
      841 gccatgatgc taacctctta attgttggag ttgccaaata acttctcaaa cgttttttta
      901 gtgtcttttg ttacggcaat ttgatttaaa tctaaagcat tgttaaaacc actgagataa
      961 ccttgtgagg tatattggtg taaatcgtaa tctaaggttg tattaggagc agcctcataa
     1021 tagccagaat cactaccata agtcggaatc catacagcat cgaaaccttt tgttgaaata
     1081 tcttgttctg ccataaaata agttccaata tagagaccaa cattttcagc accaagtttt
     1141 ttaagctctt ctcggaaggc tgttacgcct ttattcatat ctttcatagt ggcttcttcg
     1201 acatcaatcc agtagtaagt tggattatag ggagcagcgt ttttgtagaa agctcgggcc
     1261 tcttctttca tctctttagt actacgacca agtgcgtaac tgtaaacagc tacaggaaca
     1321 tttcgttttt gaaattcttt gatatgcgtt ttaaaagatt tgtcaattcc agttgtaaaa
     1381 gcagcgttgt tatgagctgt aatttgcgat cctccataga cacgtacaat tgcgccagag
     1441 atgtgacgtg acaatgtgtc ataatcaatt tcttcaggga gttgccagcc ggaaacatca
     1501 acaataggat ttaaaataaa gtcttcggtt tcagaagtgc tagtttttgc tttactagga
     1561 ttactggttg caataggaat atgactctta gcgtcttcaa cttctttttg tttcgcatga
     1621 ttagcttgtc tttttccgat aatcaatacc attgccagta aaataaagaa gactagcaca
     1681 acaataggtt ttatttttct tctcattaac tgttattgta ccctaattac taaaaaaaat
     1741 caaaatatcc taactgtttt c
//
LOCUS       DAUGHY010000107         1743 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.107, whole genome shotgun sequence.
ACCESSION   DAUGHY010000107 DAUGHY010000000
VERSION     DAUGHY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.107"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..940)
                     /locus_tag="VH752_001682"
     CDS             complement(<1..940)
                     /locus_tag="VH752_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8290009.1"
                     /translation="MNKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYS
                     SVDSSLVSVSNNASSYANRTMARISSFYFDTENNIIKALPDSDSSKLLGTPAANTDII
                     LFSANGTILNAFDAFSNYQNFHLDKYRLGSIETTSLMNFYGQEEKYHTITVRVHIKNY
                     PAVAYMMAVVNVEQLDRANERYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEK
                     QKMFVENASHELRTPLAVLQNRLESLFRKPNETILENSEHLASSLDEVRNMRILTTNL
                     LNLARRDDGINPQWTHLDTDFFNAIFENYELVAKEYG"
     gene            complement(933..1607)
                     /locus_tag="VH752_001683"
     CDS             complement(933..1607)
                     /locus_tag="VH752_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8290010.1"
                     /translation="MIKILLVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGI
                     YDLILLDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVY
                     LLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYISKIRKKLKGTRFVNRLQTLRSVGY
                     ILKNNE"
BASE COUNT          600 a          339 c          217 g          587 t
ORIGIN      
        1 ttccatattc tttagcaact agttcataat tctcaaaaat agcattaaaa aaatctgtat
       61 ctaaatgagt ccactgtgga ttaatgccat catctcgtct tgctaaattt aataaattag
      121 ttgttaagat gcgcatgttg cgaacctcgt ctaaactaga agcgagatgc tcactatttt
      181 ctaatatcgt ttcgttgggc ttacgaaaaa gcgattccag acgattctgt aagaccgcca
      241 aaggggtcct tagttcatga ctagcatttt caacaaacat tttttgtttt tcatagcttt
      301 ctaaaatagg ttttctgctc cacttggcta aataaatact tgctaaaata gaaattagcc
      361 aaaaaacact cataactata ataataatgc gctcgtaacg ttcattagcg cggtctaatt
      421 gttccacatt tactactgcc atcatatagg caactgcagg ataatttttg atatgaaccc
      481 ttacagttat cgtatggtat ttttcttctt gtccataaaa attcattaaa ctggtggttt
      541 caatactccc caaacggtat ttatctaaat gaaaattttg atagttagaa aacgcatcaa
      601 aagcatttaa aattgttcca ttagcactaa acaaaatgat atctgtatta gctgcaggcg
      661 ttcctaataa cttagaacta tctgaatcag gcagcgcctt aataatgtta ttttcagtat
      721 caaagtaaaa agaagatatt ctagccatcg tacgattagc atagctactt gcattattac
      781 taacagaaac taaactactg tcaactgacg aataaacgcc aaaccgcata atctgtaaga
      841 taataatagt cataatgaca aaaatacctg taaaaaccgc aaaaaaatga aaaaagtggt
      901 tatagttatc tgataaaatc tcttttttta atttattcat tgttttttag aatataccct
      961 acgcttctta aagtttgaag cctgttaaca aaacgggtac cttttaattt ttttcttatt
     1021 ttagaaatgt aaacttcaac aaccgaaatc gtagtatcac tatcgaatcc ccataaacga
     1081 tcaaaaattt gtgtctttgg taaaataacg ttttgatttt gcaaaagata caccaacaaa
     1141 tcaaattctt tgccaagtaa ttcaactact ttcccttcaa ccttcacctc ttttcgagct
     1201 aagtcaacca ctaaattgcc aaaactaata tttttatctg caaatttccc tgttcttttt
     1261 aacagtgctt gaattcgcat ttttaactct tctaaataga acggcttggt cagataatca
     1321 tcagcaccta attcaaatcc gtgaccttta tcatctaaac cctctttagc tgtcatgatc
     1381 aacacaggaa ttttaatatc tttttctcgc aattctttta agacttgaaa gccatttttc
     1441 tccggcaaca taaggtctaa caaaatcaaa tcataaatac cactttctgc ttcgtataaa
     1501 ccttcatcgc catcaaaaac ttgcatgacg tctgcaaaat catctaaaaa atcaaaaatg
     1561 gaatttgata agctcaagtc atcttctact aataatattt ttatcatgtc tctacctcaa
     1621 ttgttcttaa ataaagtaac tatcacttca tttaacttag tctcatttct gataacaatt
     1681 tctaacgaga ctatactaca aaaagcttaa ggtttactaa aatattaata ctcctttaag
     1741 gca
//
LOCUS       DAUGHY010000108         1740 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.108, whole genome shotgun sequence.
ACCESSION   DAUGHY010000108 DAUGHY010000000
VERSION     DAUGHY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.108"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            435..1505
                     /gene="xerS"
                     /locus_tag="VH752_001684"
     CDS             435..1505
                     /gene="xerS"
                     /locus_tag="VH752_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEQ8290011.1"
                     /translation="MRRELLLEKIETYKAIMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTQAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
BASE COUNT          588 a          274 c          325 g          553 t
ORIGIN      
        1 atagtatcaa aaaaataaaa cgaaagcaag tgtaacattt cctacaatta attcatgtga
       61 tagcaatact gtcaaaaagg ggaaacacct aaaacatagc gactagattc gtgtactagc
      121 aggtctatat ctaataaaga gttaattact ttaaagataa aattatcatt tagttctgag
      181 ctaaacaaat tctgtgcctg aagttactaa aataatgata gtaaattatt actgaatcta
      241 aaagaagtga cgttagtatc tgtaaaaaaa gagatttgtc tcgtgacatc tttctgaaaa
      301 actgtaattt tcttgaaaaa tatttatagg tgtgatatac tgctcttgta gagaatgaat
      361 gacataaaat acatgagaca atttgcgaaa attcattttt gaaaaaattg ttattgttaa
      421 ggaattatga tgatatgaga cgcgaattat tactagaaaa aattgaaacc tataaggcca
      481 tcatgccttg gtatgtttta gattattacc aatccaagtt agctgttcca tacagcttta
      541 ccaccttata tgagtattta aaagaataca aacgcttttt tgattggctg atggatgctg
      601 atttaacgca ggcgccaaag attgctgata ttgacttgag cacgcttgag caccttacca
      661 agaaggattt agaggccttt gtgctttatt tgcgggaacg accttctctc aacacctatt
      721 ccaccaaaga ggggctttct caaaccacta ttaatcggac cttgtctgcc ctatcaagcc
      781 tttacaagta cttgactgaa gaggttgaaa atgaccaagg ggagccttat ttttatcgga
      841 atgttatgaa aaaagtgtca acaaaaaaga aaaaagaaac cctggcttcc agagcggaaa
      901 atattaagca gaaactgttt ttaggtgatg aaactctagc tttcttggat tatgtggata
      961 aggaatatga gcaaaaattg tctaatcgtg ccaaatcttc tttccgtaaa aataaggaga
     1021 gggatttggc tattattgct ctactacttg cttctggtgt tcgtttgtct gaagcagtga
     1081 atcttgattt aaaagatgtc aatctaaata tgatgattat cgaagttatt cgcaaaggcg
     1141 gaaaacgtga ttcagtcaat gtagcaggtt ttgcgaaagg ttatttggag tcttatttag
     1201 ctgttcgtca gagacgttac aaggctgaaa aacaggatct tgctttcttt ttaacagaat
     1261 accgtggcgt tccaaatcga atggatgctt ctagtataga aaagatggtt ggcaagtact
     1321 ctgaggattt taaaattcgt gtgactcctc acaaattgcg tcatacgcta gcgacaagac
     1381 tatatgatgc tactaaatct caggtgttag ttagtcatca attaggccat tcctctactc
     1441 aagtcacgga tctttatacc catattgtca atgatgagca aaaaaatgct ttagataacc
     1501 tttaatgtta cataattgtt tttatgtaac attaaaggta aaaatagctt caaaattaag
     1561 tttaatagat ggctactatt aaccgtgaca gtttggtgtt aggccgtttt ccaaactact
     1621 caagccactt agaaaaagga atatcactgc taactttgtc ccttctttga atgacttttt
     1681 tgtgatgttt atagattaaa tctagtaagg ttctcatcaa aaagatgaga taagcctgtt
//
LOCUS       DAUGHY010000109         1722 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.109, whole genome shotgun sequence.
ACCESSION   DAUGHY010000109 DAUGHY010000000
VERSION     DAUGHY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.109"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            15..386
                     /locus_tag="VH752_001685"
     CDS             15..386
                     /locus_tag="VH752_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8290012.1"
                     /translation="MSWKFDEKSPIYAQIAQHVMMQIISQEIKSGDQLPTVREYAEIA
                     SVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFSHTEIIPVLTSFLKEDSQ"
     gene            386..1084
                     /locus_tag="VH752_001686"
     CDS             386..1084
                     /locus_tag="VH752_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8290013.1"
                     /translation="MAHLLQLHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            1094..>1722
                     /locus_tag="VH752_001687"
     CDS             1094..>1722
                     /locus_tag="VH752_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8290014.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFGVLTNKMLPLTLGLTFGSLIAGSLLSTLLIIIKRFSKSVFGREGYLTLTLPVNSH
                     QIILSKLLASFICSVFNTIILAFAIAIVIVPMFNINELLEGFFNSFKVDYFINMLTVL
                     AYVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYV"
BASE COUNT          556 a          307 c          293 g          566 t
ORIGIN      
        1 agaaaggaga ccacatgtct tggaaatttg atgaaaaatc tcctatttat gcgcagatag
       61 cacaacatgt catgatgcaa attattagcc aagaaatcaa gagtggtgat caacttccaa
      121 ctgttcgtga atatgcagaa atcgcaagtg ttaatcctaa caccatgcaa cgagcgttta
      181 cagaacttga acgggaagga atggtttatt cgcaacgtac tgctggccgt tttgtaactg
      241 acgatcaaaa attaattgct cgtaaaagac gagagttagc aattagtgaa ttagaatcat
      301 ttatcaccaa tatgacaaaa atgggattta gtcacactga gattatccct gttttgacga
      361 gctttttaaa ggaggattca caataatggc acacctattg caactacatc atgtttcaaa
      421 atcttaccgt gaaaaaaaag ctattgatga cttaaccatt actatcccga acggtaaaat
      481 tatcggatta ttaggtccaa atggaagtgg taaaacaact ttaattaaac tcattaatgg
      541 ccttttacaa ccaaataaag gagaaattgt cattgatggc taccgtccct gtgttgagac
      601 aaaaaaaatt atttcttatt taccagatac tacttatctc aatgaaaata tgagaattaa
      661 ggacatgttg gagttcttta gtgattttta cagcgacttt gacaaatcca aagcaacaag
      721 ccttctaaga gatttagaac tagaccctga agaccgcttc aaaactctat ccaaaggaaa
      781 caaagaaaaa gtacaattaa ttcttgtgat gagtcgtaaa gccagactct atgtacttga
      841 tgaacctatt ggtggagttg accctgctgc aagagactac atcctaaaaa ccatcattaa
      901 tagttattgt gaaaatgctt ctgttattat ctcaacccac ttgatttcgg acattgaacc
      961 tatccttgac gaagtcattt tcttgaaaca aggaagactt ttcttatctg gcaatgctga
     1021 tgatctaaga caagaatacc agcaatccat tgattccctc tttagggaaa cttataaggt
     1081 ttaggaggat atcatgtttg gaaaattatt aaaatacgaa tttagatcta ttggaaaatg
     1141 gtactttgcg cttaatgctt tcgttattgc catcgctgcc attttgtcat ttacgataaa
     1201 actgtttgct caaagtaata gtgatggact atttggagta ctaaccaata agatgttgcc
     1261 tctgacatta ggtttaactt ttggctccct gatcgctggt tccctcttat caacattact
     1321 tatcattatc aaacgtttta gcaaaagtgt ttttggacgg gaaggatact taacgttgac
     1381 tttacccgtc aattcgcatc aaattatttt atcaaaacta ctagcttctt ttatttgcag
     1441 tgttttcaat actatcatcc ttgcatttgc tatcgctatt gtaattgtac caatgtttaa
     1501 catcaacgaa ctattagaag gattctttaa tagttttaag gtggattatt tcatcaatat
     1561 gctaactgta ctagcctatg tcctattatc aacatttacg agtatcttat taatttatct
     1621 ttccatttct ataggtcaac ttttttccaa tcggcgaggc ttgatggcct ttattgcata
     1681 ttttatatta gttattctga ttagcgttgc tgcaacatat gt
//
LOCUS       DAUGHY010000110         1711 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.110, whole genome shotgun sequence.
ACCESSION   DAUGHY010000110 DAUGHY010000000
VERSION     DAUGHY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.110"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..1282
                     /locus_tag="VH752_001688"
     CDS             <1..1282
                     /locus_tag="VH752_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEQ8290015.1"
                     /translation="EVGLIEESYSLLFDRFLEDLSEIRSCFNISEPLSNVAFSLGDSH
                     SKKQTVVKIAFKEKSVYYKPKSYHSHSILLELTSLLKSSNIPSFSLPKSLVKADYCWQ
                     LGVAYTSSNKDEVAKIYFKYGVLAAFSEIFSITDLHMENVIVSGGDLYLIDVETFFQR
                     KLNVQNQNFEGITVDTYQRIYETSLSNGLFPVQFEKNSAPNVSGISGKGGKRKKGKYE
                     LINKNRGDMKLVKTDYFQEDGYNIPTLNGKVVEPLDYANEVIAGFRECYTFLMSQRAK
                     VKKILEDFPKLKTRAIFRNTSDYGKFLQASTNPKYLFSEKKRENLFSILHESKHIEQF
                     IVVSEIKDLMNGDIPYFSMDTRGNVYNSVGTLIGNLGDTTSLFDSITTLNDERLKFTC
                     ELLEIVLKKPIKHWEREKGKSYQFLSISSEHYLL"
     gene            1284..>1711
                     /locus_tag="VH752_001689"
     CDS             1284..>1711
                     /locus_tag="VH752_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ8290016.1"
                     /translation="MDKTDFRNISAFVSQDSPIFDGDVMYNISLGRESVSGEQVIETC
                     KRVSLYEDIRSMPMKFHTPLFRDNPSLSGGQKQRISLARELVTTPRILVLDEPTSALD
                     VKTERIIQKNVEALHCTRVLVTHRLNTVEKADKILIMDNG"
BASE COUNT          604 a          239 c          325 g          543 t
ORIGIN      
        1 agaagtagga ctgatagagg aatcatattc tttattattt gatagatttt tagaagactt
       61 atctgagata aggtcgtgct ttaacattag tgagccccta agcaatgttg ccttttcact
      121 tggtgacagt cactctaaaa agcaaacagt tgttaaaatt gcgtttaaag agaaatctgt
      181 ttactacaag cctaaaagtt atcattcaca cagtatcttg ttagaactta ctagtttatt
      241 gaaatctagt aatatacctt cattcagtct tcctaaaagc ttagttaaag cagattattg
      301 ttggcaactt ggtgtagctt atacaagttc caataaagat gaagtagcga aaatttactt
      361 taagtatggt gttttagcag cattttctga aatattttct attacggact tacatatgga
      421 aaatgtgatt gtttcaggcg gagatttata tttaattgac gttgaaacat tttttcaaag
      481 aaagttgaat gtacaaaatc agaattttga agggattact gtagacacct atcagagaat
      541 ttatgagaca tcactttcaa acggtttatt tcctgtacaa tttgagaaaa acagcgctcc
      601 taacgtttct ggaataagtg gaaaaggtgg aaaaagaaaa aagggaaagt atgaactgat
      661 aaataaaaat cgtggagata tgaagttagt gaaaactgac tattttcagg aagatggtta
      721 taatattccg acattaaatg ggaaagtggt ggaacctctc gactatgcta atgaggtaat
      781 tgcaggtttt agagaatgct atacatttct catgtcgcaa cgtgcaaagg ttaaaaaaat
      841 tttagaagat ttcccaaaat tgaaaactag agccatattc agaaatactt ccgactatgg
      901 aaaattttta caagcctcta cgaatccaaa atatcttttc tcagaaaaaa agagggagaa
      961 tttattttcg atactacatg agtcaaaaca tattgaacaa tttattgttg ttagtgaaat
     1021 taaagatttg atgaatggtg atattcctta cttttcaatg gataccagag gaaacgtcta
     1081 taattctgta gggacattaa ttggaaattt aggagataca acctcgttat ttgatagtat
     1141 aactactttg aacgatgaaa gattgaagtt cacttgtgaa ttgcttgaaa tagtcttgaa
     1201 aaaaccgatt aagcattggg agagggaaaa gggcaaatct tatcagtttt tatcaatctc
     1261 ctcagaacat taccttctct aaaattgata aaactgattt taggaatata tcggcttttg
     1321 tatctcaaga ttctcccata tttgatgggg atgtgatgta taacatttcg ctagggagag
     1381 aatctgtttc aggagaacag gttattgaaa cttgtaaaag ggtatcacta tatgaggata
     1441 tcaggagtat gccaatgaag tttcataccc cactttttcg agacaatcca tcactatctg
     1501 gggggcaaaa acaacgaatt tctctagcaa gagagttagt aactacccct agaatcttag
     1561 ttcttgatga acctacatca gctttagatg taaaaactga aagaataatc caaaaaaatg
     1621 ttgaggcttt acattgtacg agggttttgg ttacccatag acttaataca gttgaaaaag
     1681 ctgataagat tttaataatg gataatggga a
//
LOCUS       DAUGHY010000111         1676 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.111, whole genome shotgun sequence.
ACCESSION   DAUGHY010000111 DAUGHY010000000
VERSION     DAUGHY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.111"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(110..415)
                     /locus_tag="VH752_001690"
     CDS             complement(110..415)
                     /locus_tag="VH752_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEQ8290017.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            complement(427..846)
                     /gene="ruvX"
                     /locus_tag="VH752_001691"
     CDS             complement(427..846)
                     /gene="ruvX"
                     /locus_tag="VH752_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEQ8290018.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            complement(843..1112)
                     /locus_tag="VH752_001692"
     CDS             complement(843..1112)
                     /locus_tag="VH752_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEQ8290019.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            complement(1225..1623)
                     /gene="spx"
                     /locus_tag="VH752_001693"
     CDS             complement(1225..1623)
                     /gene="spx"
                     /locus_tag="VH752_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEQ8290020.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
BASE COUNT          501 a          344 c          241 g          590 t
ORIGIN      
        1 aatttatcat ttaattcttt tatcattatc acctcctgct tatttattcg aaaataacat
       61 taaaaaaata gccctcacgg ctacttttta acatagtgtc actaacaatt tagttctcat
      121 ctagaaaact attaaatacc tcttcaatca tatcccattc agcatctgaa tcctcaggaa
      181 tcggttgtaa atccccttca gtaccatctt cattttcagt aaatgaatag gcttggattt
      241 caatttcacc agactcatct tcttcagatc ccgctggaac caagagaaca taatttttac
      301 caaactcttc acgaccatca attgttaata aaatttcaaa caaggtttca tttccttgtt
      361 catccacaag tgtgataact tcgtgttggt ggtcattttc atgattatgt gtcatttttt
      421 atctccttaa aaattccgat ttaaatagtt ctgtaaaatg agttgtgcag ctaatttatc
      481 aatgactttt tttcgcttac cacgactaat gtcagcttgc tcaataagca tacgctctgc
      541 ctcaacagtc gtcaatcgtt catcttgata atggactgga agtccaaaaa ggtgctctat
      601 gtggtttcca taagttatac ttgcatcaac acgtggacca ttggtgttat tcatattttt
      661 tggcaatcca ataacaaact gttcaacttg gtactgctta acaagctctt ctaaacgtgt
      721 aaagccaaat tctgcttttt cctcatcaat cttgataatt tcaagtcctt gggctgtaaa
      781 gccaagagga tcactaatag ctacaccaac cgttttcgag ccaacatcaa gtcccattat
      841 tctcatttaa catcaattcc atttccttgt aaatagtagc gaacaagttc ttctacaatt
      901 tcatcacgtt catatttacg aatctgattt cgagcatcat tataacgagg aacataagct
      961 gggtccccac tcaagacata accaacaatt tgattaattg gattataacc tttttcatca
     1021 agtgaatgat agactgctgt taatgtttca ctaatttgtc gtttgtcgcc atcatccaac
     1081 ttaaagcgga ctgtttcatc tgtaaatccc atacttacac cttctttcta aaattaagtg
     1141 ctatgcttta ttatagctta tttctattaa ataaacaaga atttcaagcg aagaaggtat
     1201 ttaaaaaaag ctctacaaga gcttttagag cgcagcacgt aatcgtgctt cagtattttc
     1261 aatattacga atagagcgag gtaaaaaagc acgaatatcg tcttctttgt agcctacttg
     1321 cagacgttta tcatcaatca aaatgggact tttaaggatg cgcgggttat cttgaatcaa
     1381 atcaataacc tcactaacac ttaattcttc aatatcgcaa tcgagagctt tggcataacg
     1441 gtttttagat gaaacaatgc tctccactcc attttcggtt tttgataaaa ttgctaaaat
     1501 ttcttcttta gttagcggtt cttttcctaa gttctgttct ttataagcaa gcttatgggc
     1561 atttagccaa gttttggctt tcttgcagct cgtacaactt gaaatcgtgt aaattttaat
     1621 catgttgctg actcactttc tatttcgtga tagctttatt gtagcacagt ttctta
//
LOCUS       DAUGHY010000112         1647 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.112, whole genome shotgun sequence.
ACCESSION   DAUGHY010000112 DAUGHY010000000
VERSION     DAUGHY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.112"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            13..1131
                     /locus_tag="VH752_001694"
     CDS             13..1131
                     /locus_tag="VH752_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8290021.1"
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQA
                     KIGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSD
                     KVLQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQ
                     LNYDVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSSQLYHRLMLSN
                     LSRFRYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFG
                     LLIGAITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIA
                     IFQSGETLSQAWPYLLALMGTALALISFSSLLLKPTKL"
BASE COUNT          533 a          243 c          286 g          585 t
ORIGIN      
        1 gcaggtgacg acatgatttg ttttataaaa actctatttg ttaagataaa gcgcaaaaaa
       61 acaagttatg tgacattctt tttaatgcct attttaacga cacttttggc tttatctttg
      121 agtttttcaa ataataatca ggctaaaatt gggatattag ataaagataa tagtcagata
      181 tctaaacaat ttattgctca actaaagcaa aacaaaaagt acgacatttt taccaaaatc
      241 aaaaaagagc atattgatca ctaccttcag gataaatctc tagaagcggt attgactatt
      301 gacaaagggt tctcagataa agtgttgcaa ggaaaatctc aaaaactcaa tatacgttcg
      361 attgctaata gcgaaattac agagtgggta aaggcacaga ccaattatct attggaaaat
      421 tataatatta taggggatgt ggctctaggt aacgaagata cttttaatag aattctccaa
      481 aaaaaccaac agttaaatta tgatgttaag caagtaactt tgacagaccg atcacgtagc
      541 aaagcagtat cttccacaac gacaggattt ttgcttattc taatgttagg tagtacgagc
      601 gtgatttaca gtggtatttt ggcagataaa tctagtcagt tataccatcg attaatgtta
      661 tcgaacttat ctcgttttcg ctatatgttg agttacgtct gtgtaggatt tgttgctttt
      721 acgattcaaa ttgtcattat gctgagtttg ttaaaagtat ttaatattag tttttttgtt
      781 ccgacatcac ttcttttgat tatttttttc ctttttagtt tattagcgat aggctttggc
      841 ctgctgattg gagctatcac acaaaattcc caacaaagta gccagttagc taatcttatt
      901 gtaatgccga cttcaatgtt ggctggctgt ttatggccat tgtctattac gccttcttat
      961 atgcaagcaa ttggtaagtt attaccacaa aattgggttt tatcagccat agctattttt
     1021 caaagtggag agaccttatc tcaggcttgg ccatacttgt tagctctaat gggaacagca
     1081 ctagctttga ttagtttttc aagtttatta ttaaaaccta ccaaactata attagtcttt
     1141 tgttaaaaat actttcctaa ttagctaact accaggtagc ccttttattg gcttatatgc
     1201 aataaaaggg ttgctttttg cttatatttt ctctagtgca cttatctttt gttaaaagcg
     1261 attgcgatgt tactgttgtt actgtgtttt ttagagagca tattatcaaa agcgacctct
     1321 ctagaaagtt cgtttttgtt gataaatgag ttgtcaaagc attttaaaaa aacgttttca
     1381 ataaacataa aaactaatgt tattaaaaaa aggtgtaaaa acgtatttat tttatgaaat
     1441 gtttttgtta caataagcat gtatcgaatg ctataaagct gtcttgttcg ctatcagggg
     1501 tgcttgtgat gttatgtcag atcaaacaag tctttaattg ggtgacttaa cagattaaaa
     1561 gaaaagatct aatcgtgtta cttgatagta taattaatgc aaacagaaag tgtatcccaa
     1621 ttcatagttt caggtggagg ttaatat
//
LOCUS       DAUGHY010000113         1615 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.113, whole genome shotgun sequence.
ACCESSION   DAUGHY010000113 DAUGHY010000000
VERSION     DAUGHY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.113"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            115..1596
                     /gene="guaB"
                     /locus_tag="VH752_001695"
     CDS             115..1596
                     /gene="guaB"
                     /locus_tag="VH752_001695"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEQ8290022.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
BASE COUNT          457 a          286 c          371 g          501 t
ORIGIN      
        1 cttcagctgt aggaatattt tattgacaaa tgattttagc gtgatatgat agtaacaata
       61 aaaaatagac cagggtaata ttccctaaaa ataaaagaaa agaggattat ctggatgtca
      121 aattgggaca ctaaattttt gaaaaaaggc tacacttttg acgatgtctt gcttattccg
      181 gcagagagtc atgttcttcc aaatgaagtt gatttaaaga caaagctcgc agacaatttg
      241 acattaaata ttcctattat tacagcagct atggatacgg ttactggtag taaaatggct
      301 attgcaattg cgcgtgcggg tggtttaggt gttattcata aaaacatgtc tattactgag
      361 caagcagaag aagttcgcaa ggtaaaacgt tctgaaaatg gtgtcatcat tgatccattc
      421 ttcttaacac ctgaacacaa ggtttcagaa gctgaagaat taatgcagcg ctaccgtatc
      481 agtggtgtgc caattgttga aactttagca aaccgtaagt tggtcggtat cattactaat
      541 cgtgatatgc gttttatttc tgattacaat gcaccaatct ctgaacatat gactagtgag
      601 cacctcgtta cggctgctgt tggtacagat cttgaaactg cagaacgtat tcttcatgaa
      661 caccgtattg aaaaacttcc tttggttgat aatagcggtc gtctgtctgg tttaatcact
      721 atcaaagata ttgaaaaagt aatcgaattc ccacatgctg ctaaagatga atttggccgt
      781 cttctggtcg ctgctgcggt aggtgttacc tcagatactt ttgagcgtgc cgaggccttg
      841 tttgaagctg gtgcagatgc gattgttatt gacacagctc atggtcattc ggctggtgtt
      901 cttagaaaaa ttgctgaaat tcgtgctcat ttcccaaatc gtactttgat tgctggaaat
      961 attgccactg ctgaaggtgc gcgtgctctt tatgatgcag gtgttgatgt tgttaaagtg
     1021 ggtattggtc ccggatctat ctgtaccact cgtgtggttg caggtgtcgg tgttcctcaa
     1081 gtgactgcta tttacgatgc tgctgctgtt gctcgtgaat atggcaaaac gattattgct
     1141 gatggtggta tcaagtattc tggcgatatt gttaaggctc ttgcagccgg cggtaatgct
     1201 gttatgcttg gatcaatgtt tgcaggtact gatgaagctc caggtgaaac tgaaatctac
     1261 caaggccgta agttcaaaac ttatcgtgga atgggttcca ttgcagctat gaagaaagga
     1321 tcaagcgatc gttacttcca aggttccgtc aatgaagcta ataaactagt tccagaaggt
     1381 attgaaggcc gtgttgctta taaaggagct gcttcagata ttgtcttcca aatgttaggt
     1441 ggtatccgct caggtatggg ctatgttggc gcaggtgaca ttcaagaatt acatgaaaat
     1501 gctcaatttg ttgaaatgtc aggtgcaggc ttgattgaaa gtcatccaca tgatgtccaa
     1561 atcacaaacg aagcaccaaa ctattctgtg cactgataat ttaatttagt aactc
//
LOCUS       DAUGHY010000114         1578 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.114, whole genome shotgun sequence.
ACCESSION   DAUGHY010000114 DAUGHY010000000
VERSION     DAUGHY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.114"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            17..937
                     /locus_tag="VH752_001696"
     CDS             17..937
                     /locus_tag="VH752_001696"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEQ8290023.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNQKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKCDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            991..1239
                     /locus_tag="VH752_001697"
     CDS             991..1239
                     /locus_tag="VH752_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEQ8290024.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(1364..>1578)
                     /locus_tag="VH752_001698"
     CDS             complement(1364..>1578)
                     /locus_tag="VH752_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8290025.1"
                     /translation="ISVIDNGPGITDEEKKKIFDRFYRVDKARTRQTGGFGLGLALAQ
                     QIVMSLKGNITVKDNDPKGSIFEVKL"
BASE COUNT          555 a          297 c          225 g          501 t
ORIGIN      
        1 atgattagga gctaccatgt caaatgaatt tatcaacttt gaaaaaattt ctagagagtc
       61 ttggaaaaca ttacatcaaa aagcaaaagc tcttctcaca caagaggaac ttaaaagtat
      121 tactagctta aatgataaca tcagtattaa cgatgtcatt gatatctacc ttccattaat
      181 taatctgatc caggtttaca aaattgcgca agaaaatctt tctttttcta aaagcctttt
      241 tctaaaaaaa gacattcaat taaggccttt tattattgga atttctggtt cagtagctgt
      301 tggtaaatca acaactagtc gtcttttaca acttttatta tcacgaacgc accccaatag
      361 tcaagtcgaa ctggtaacga ctgatggttt tttatatcct aatcagtttc ttattgaaca
      421 aggactgtta aatcgtaaag gatttccaga atcttataac atggaacttc tcttagattt
      481 tctagattcc atcaaaaatg gtcaaactgc ttttgcaccc gtctattctc atgatattta
      541 cgatattatc ccaaatcaaa aacaatcatt taataaccct gacttcctta tcgtcgaagg
      601 tattaatgtt tttcaaaatc aacaaaacaa tcgtttatat atgagtgatt actttgattt
      661 ttctatttat attgatgcag acagcagtca catcgaaacc tggtacattg aacggtttct
      721 aagcatttta aaattagcaa aatgtgaccc tcataattat tatgcccaat atgcacaatt
      781 acctcgatct gaggctatag catttgcgcg caacgtttgg aaaaccgtta atttagaaaa
      841 tcttgaaaaa ttcattgaac ctacacgtaa ccgtgctgaa ttaattttac ataaaagtgc
      901 tgatcataaa attgatgaaa tttacctcaa aaagtgataa agggttgcca aacgcatata
      961 ttttctatat aataatgtag ttagattaat ttggaggtga aaacattggc aaatattaaa
     1021 tcagctatta aacgcgctga acttaacgtt aaagcaaacg aaaaaaactc agcacaaaaa
     1081 tcagctatgc gtactgctat caaagcattt gaagctaacc catctgaaga gcttttccgc
     1141 gctgcttctt caagcatcga taaagctgaa tcaaaaggtt tgattcacaa aaacaaagca
     1201 agccgcgata aagcacgcct tgctgcaaaa cttggctaat aaatatccaa tgaaaactcc
     1261 agctggagtt tttttatacg gtaaaaactc aaaaaataca ataaagcgtt gacagttaaa
     1321 aataacttta aaagggctga ccttattggt cgcccttttt agcttataat ttgacttcaa
     1381 aaatactacc tttaggatca ttatccttta ctgtaatatt tccttttaaa gacatcacga
     1441 tttgctgagc taaagccaac cccaagccaa atccacctgt ttgccgtgtt ctagctttgt
     1501 caactcgata aaaacgatca aaaatctttt tcttttcttc atctgttatc cctggaccat
     1561 tatcaataac agaaatta
//
LOCUS       DAUGHY010000115         1543 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.115, whole genome shotgun sequence.
ACCESSION   DAUGHY010000115 DAUGHY010000000
VERSION     DAUGHY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.115"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..>1543)
                     /locus_tag="VH752_001699"
     CDS             complement(<1..>1543)
                     /locus_tag="VH752_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEQ8290026.1"
                     /translation="YHLDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYRIIA
                     DQEISFYLFRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKLYLP
                     EFIVGELSKITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQKTKK
                     KHVLLWMKLFAINGLTLVVLSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELIFFT
                     TIFFKLCSAMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFDTSH
                     SKTVIAHRGLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKRLAG
                     VNKTIRNLTLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEPGNY
                     VDLFLETYHRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFVDFY
                     VIEDFSYRSYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTNQLIKD
                     LKQDNS"
BASE COUNT          565 a          266 c          237 g          475 t
ORIGIN      
        1 aagaattatc ttgttttaag tctttaatca attgattagt taaagctggt tgatctgtaa
       61 taattccctg aataggcttt aggagataat gctctatgcg cttgggatca ttaatagtcc
      121 aaacgtaaat ttctttatta ttccaaaaag cttgggacga caaataagac cgataagaaa
      181 agtcttcaat gacatagaaa tcaacaaatt catctccaaa aaagccaaat tgaattggta
      241 tgatataacc agtcgtaatt gatggatttt ttttcttgat agcttctatt acttctaaat
      301 ctaaagacat gactttatta tctttgctta taccaagtcg atgataagtt tctaaaaaca
      361 aatcgacata atttccaggt tctgtaccaa ttggcttgag ttcaataagt aatggcatat
      421 tcaacttctt agccttttga taaaaagtgt caaaagaaac aaaacgccct gaaaaatgtc
      481 cttgatgact cgttagatgt tcaacttctt ttaaggttaa gttgcgaatc gtcttattta
      541 ctccagctaa acgcttcaat cgattatcat gagacaccac aaagtgatta tccttagtca
      601 agattagatc cagttctaca taatcacttc ctgctttctt agcaccttca agggcttcca
      661 gagaattttc tacacctgct gatacaagtc ctctatgcgc gataactgtc ttagagtgag
      721 atgtgtcaaa aaaagttaaa cgttcaagag attgataagc aaaacctact gtcaccacga
      781 taaagattac aacatatgcc ttattacttc ttcttggctc atcatattgc ttttgtggtt
      841 caattgcctc ttttaaaatc attgctgaac agagtttaaa aaaaatagta gtaaaaaaaa
      901 tgagctccca tgtaaaggtc aaagctccca gctgaacaat aatattattc cccttaggat
      961 taaacatatc aacaaaaata agaatcatgg aaatagctag cgataagact acaagcgtaa
     1021 gaccattgat tgcaaaaagt ttcatccata acaatacatg tttcttttta gtcttttgcc
     1081 aactctctct ccatgcttga gcaaccgtac gatggttgat tgctatcaat ggtaaaaaat
     1141 atactaatct taggttaagg taaaacacaa gaataagact gccataaagc aagtactttg
     1201 tgctagttat ctttgataat tccccaacaa taaattctgg aagataaagc ttttgagtaa
     1261 tcacagaaga taaaccaata tgtaagactg gaatagtcag taggatataa agcaaaaaaa
     1321 gtaataattg gtaaccagaa aatgttttcc aaagccccct taggtaataa gaaaactgtt
     1381 ttctaaaaag atagaaacta atttcttgat ccgcaataat tcgatataga gcaaaaaact
     1441 caaggtgaat aaaagcagct aataaaaata aataggcacc taataaagca agtgctatag
     1501 gattttttaa aaaagtcaca acattgtctt tatctaaatg ata
//
LOCUS       DAUGHY010000116         1491 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.116, whole genome shotgun sequence.
ACCESSION   DAUGHY010000116 DAUGHY010000000
VERSION     DAUGHY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.116"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..575
                     /locus_tag="VH752_001700"
     CDS             <1..575
                     /locus_tag="VH752_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ8290027.1"
                     /translation="VLPYTRPRGKKDLLRPKEFIYDEHFDCVICPEHQALTYRTTTRE
                     GYREYKSDPAICANCPLLSVCTTSKNHLKVVTRHIWKDYLEQCEDIRHQRGMKELYQH
                     RKETIERLFGTAKEYHNLRYTREKGKSKMEDKVGLTLACLNLKKLVKRMAGEPFYFVQ
                     MRWFKHGNSHFYLKTLKKTNTKSMFVFNLF"
     gene            complement(823..>1491)
                     /locus_tag="VH752_001701"
     CDS             complement(823..>1491)
                     /locus_tag="VH752_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein"
                     /protein_id="HEQ8290028.1"
                     /translation="VAVALLGATQPISASADEASNSIYNTYMEGYDYSAYYDAYMEGY
                     KEGLSGEPIPDALDTWPSDYKIPYLEGYLKGKEEHSKSNAEVPSGKNPHSQNEPRMPE
                     NNIPSPGKDTIPEPPHSEWPQRDPNFGGLSFGQSSDWGRSEDNPNSPSEPRIPGSSIP
                     SPRLPQASENPKEDTSTLTYEKGLEDGRKAKLTNKIDYSRLSDRPDNPFPRDYVRGYF
                     EGIK"
BASE COUNT          431 a          309 c          300 g          451 t
ORIGIN      
        1 ctgtccttcc gtacacacga ccaagaggaa agaaagacct tctacgtccg aaagaattta
       61 tttatgatga gcactttgat tgtgtcatct gccctgaaca tcaagccttg acctaccgca
      121 ctacgaccag agaaggctac cgagaataca aaagtgaccc tgcaatttgt gccaattgtc
      181 ccctgttatc tgtatgtacg accagtaaga accacctaaa agttgtcaca cggcatattt
      241 ggaaagacta tttggaacag tgcgaggaca ttcgtcatca aagggggatg aaggagctct
      301 accagcatcg gaaagagacg attgaacgac tgtttggcac agccaaggaa taccacaatc
      361 tccgctatac gagagagaaa ggcaagtcca aaatggaaga taaggttgga ctgactttgg
      421 catgcttaaa tctcaaaaaa ctggtcaaaa ggatggcggg agagcctttt tattttgttc
      481 agatgaggtg gtttaagcac ggaaacagcc atttttatct aaaaacacta aaaaagacaa
      541 acaccaaatc gatgtttgtc ttcaatctgt tctagagact ttttctttat tctgagcgat
      601 agttgtcata ccatgctttt ttagatgagt acactgcact cttttataat ggttttagtg
      661 tccgtggaat aaagagttca gcattatact cactattgtt aacttcacta tcactttatt
      721 caatgtatca tagtctaacc aagattgcta gttttcccaa tgatagtcac cagcaattca
      781 ggtagtggca atatgctacc cgtctcctaa gcaagaatct ttctatttaa taccttcaaa
      841 ataacctctt acataatccc ttggaaaggg gttatcgggt ctatcagaaa gacgtgagta
      901 atctatcttg tttgtcaatt ttgctttacg accgtcttcc agcccttttt cataagttaa
      961 ggttgatgta tcttcttttg gattctctga agcttgtggt aatcttggcg atggtatact
     1021 acttcccgga atacgaggtt cacttgggga attagggttg tcctcagatc ggccccaatc
     1081 agaggattgg ccaaatgata atccaccaaa gtttggatcc ctctgaggcc actcactatg
     1141 aggaggttca ggaatagtat ctttccctgg cgatggtata ttattttccg gcatacgagg
     1201 ttcattttgg gaatgagggt ttttaccaga aggaacttca gcatttgatt tactgtgctc
     1261 ttcttttcct tttaaataac cttctagata aggaatttta taatctgacg gccacgtatc
     1321 taaagcatcc ggaataggct caccagaaag accttcctta tagccctcca tataggcatc
     1381 ataataagca gagtaatcat agccttccat atatgtatta tatatgctat tacttgcttc
     1441 atctgctgat gctgaaattg gttgtgtagc tcctagtaga gccacggcta c
//
LOCUS       DAUGHY010000117         1412 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.117, whole genome shotgun sequence.
ACCESSION   DAUGHY010000117 DAUGHY010000000
VERSION     DAUGHY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.117"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            217..1338
                     /gene="mnmA"
                     /locus_tag="VH752_001702"
     CDS             217..1338
                     /gene="mnmA"
                     /locus_tag="VH752_001702"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEQ8290029.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDENGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSHVTVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
BASE COUNT          438 a          235 c          308 g          431 t
ORIGIN      
        1 ttataataaa aaattattga gcaataaaat gattggcatt ccacgcagta attgttataa
       61 taaggtttgc gctgatggcg cttcaaaatc gaccttcaaa tcgacttttg aaaacgacga
      121 ccttcaatcg tacgttaact gataagaaaa agaatcataa tattcataat atgatgatta
      181 tctttttcta tcttattatt gatgggagaa ttttttatga cagataactc aaagatacgt
      241 gttgtcgttg gtatgagtgg tggcgttgat tcatctgtaa cagctctact tttaaaggag
      301 caaggttatg atgttattgg tgtcttcatg aaaaactggg acgatacaga tgaatttggt
      361 gtttgcacag ctactgaaga ttacaaagat gtagcagccg ttgctgatca aatcggtatt
      421 ccttactatt cagtcaattt tgaaaaagaa tattgggaca gagtatttga atatttcttg
      481 gccgaatacc gagcaggtcg tactccaaat cccgatgtca tgtgtaataa ggaaataaaa
      541 tttaaagctt tccttgacta tgccatgact ttaggtgcag attatgtagc aacgggacac
      601 tatgctcaag tcaagcgtga tgaaaatggt acggttcaca tgttacgtgg agctgataat
      661 ggcaaagacc aaacctactt tttaagtcaa ctatcacagg aacaactaca aaagacttta
      721 ttcccccttg gtcatttgca aaagtcagaa gttagagaaa tcgctgagcg agcagggctt
      781 gcaacggcta aaaagaaaga ttcaacaggt atttgtttta ttggtgaaaa gaatttcaaa
      841 cagtttttga gccagtactt accggcacaa aaaggtcgca tgatgactat tgatggtcgc
      901 gatatgggag aacatgccgg ccttatgtat tatactattg gtcagcgtgg tggtctgggc
      961 attggtggtc agcatggtgg ggataatcaa ccttggtttg ttgtcggtaa agatttgtcg
     1021 caaaatattt tatacgttgg tcaaggtttc taccatgaag ccctaatgtc aaatagtctt
     1081 gacgcctcag tcatccattt tacacgtgag atgccagaag agttcacatt tgaatgtact
     1141 gctaaatttc gctatcgtca acccgattca cacgtgacgg tgcatgttcg aggagataag
     1201 gcagaagttg tttttgcaga gccacagcgc gcgattactc ctggtcaagc tgttgttttc
     1261 tatgacggaa aagaatgcct tggtggtggt atgattgaca tggcttataa aaatgggcag
     1321 ccttgtcagt acatttaatg aaccttttat taagataaaa agaaagggac attcaacaat
     1381 tcctttgaca tcatatgata ttatttataa aa
//
LOCUS       DAUGHY010000118         1333 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.118, whole genome shotgun sequence.
ACCESSION   DAUGHY010000118 DAUGHY010000000
VERSION     DAUGHY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.118"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            1..746
                     /locus_tag="VH752_001703"
                     /pseudo
     CDS             1..746
                     /locus_tag="VH752_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            941..1012
                     /locus_tag="VH752_001704"
     tRNA            941..1012
                     /locus_tag="VH752_001704"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:974..976,aa:Glu,seq:ttc)
     gene            1046..1119
                     /locus_tag="VH752_001705"
     tRNA            1046..1119
                     /locus_tag="VH752_001705"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1080..1082,aa:Asn,seq:gtt)
BASE COUNT          426 a          259 c          249 g          399 t
ORIGIN      
        1 ccagaaaatc atcctattct catctttcag ccacagaacg gggtgaaatt gctgcttatc
       61 ttaaaatggg aaagaaaccc gttgagattg ctcgactcct gggtcgtcac cgttctacaa
      121 tctgccgaga aataaaacgt ggttcagtag atcaggtaaa ggataagaat ggaaaacaaa
      181 ccttcttcaa cgcctatttc gctgatagtg ggcaacgtgt ctattaaacc aatcgatttg
      241 actaagatgg ttagtatcag aaaacgttct aagaaagtca tgaagacgaa taagaagact
      301 ttaggtaaat ctatcgaaga acgtccagaa tatattaatg atcgttctga atttgggcat
      361 tgggagattg atttggctct cggcaagaaa accaaaagtg aagctgttat gttgactttg
      421 gtagagcgcc agacacgcta tgcactatag aagacaagca gtcccaaacc attaaccgtg
      481 ctgtcacgca tctcatcagt cagtatccta ttgcatccat cacagcggat aatggttctg
      541 agtttagttt actctcagac ttaaaagctg ttgacgttta ctttgcacat ccctattctt
      601 cacatgagag aggaacaaac gagaacttca atggtctcct cagagaatat gtccctaaag
      661 gaatctcact taatccacta acctctgaag aacttgacaa tgatattact gccatcaatg
      721 agcgcctaag acgacttctt caatagcaat cctcaaaatt cctgtttgag ctatcccgaa
      781 cagcttaacc tctggaactc tagttatcta tgaactcgtt gcacttgact tgacaacttg
      841 cggtttaaaa aaaatcaaat tttttttaaa aaagtataga caaaaaaaat caatctgcta
      901 taatagtaag agtgttaagg agatatcctc caaaacttct ggtccgttgg tcaaggggtt
      961 aagacaccgc cttttcacgg cggtaacacg ggttcgaatc ccgtacggac tatcgcaatt
     1021 gtcattttgg caattttttt ctttttccgg catagctcag ttggtagtag cgcatgactg
     1081 ttaatcatga tgtcgtaggt tcgagtccta ctgccggagt gataagatat ttcctaagat
     1141 aatcttagga tttttttatt tagtaaaaca gatactcagg tcaaatgctc aatttactaa
     1201 ttattaaacc actaaataca gcattgtgaa ctaacaggat tgtattttgc attaaaatag
     1261 aagttctgtt ctttaattgt atagatgata acattaagtt taattacaaa cattaactga
     1321 agcttaattt ttt
//
LOCUS       DAUGHY010000119         1313 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.119, whole genome shotgun sequence.
ACCESSION   DAUGHY010000119 DAUGHY010000000
VERSION     DAUGHY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.119"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..708
                     /locus_tag="VH752_001706"
     CDS             <1..708
                     /locus_tag="VH752_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEQ8290030.1"
                     /translation="LAECLLIEHSDTLLIHAAAVYNPLSGHSILLLGDKGAGKTTVAI
                     RLCIEHGYHLIGNDQVIFGSNSGILLTYAGTSFFKIRRTAVLSDNLLFKLFSKFFNRS
                     LQFNKASWDDKISIFPKELGIRTCNFSTEISKIYYIKTDKKEKQIYHSIWNDSLASLY
                     LNELLGRHISGQVACFQSDNGKYFSTMPLINYEKNNRVREQIVKGIFAKQKLFKITAA
                     TPAKIAEAIIENVKKNS"
     gene            763..>1313
                     /locus_tag="VH752_001707"
     CDS             763..>1313
                     /locus_tag="VH752_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ8290031.1"
                     /translation="MFVIGIPTLNEADNISRLVKQIDEYAVNLGKEIIIINSDSKSTD
                     GTPQIFLETKTYNTKVSIVSEAKGKGYNVRNIFEYAINHVPNFSGLILIDGDVVSMKK
                     MWLEKMFIAIESGNDLIIPNYARKSFEGNATNHFIYPMLVKFFKRDMPYQCISGDFGF
                     SRGLIKDLTLKCNWHKYTLGYGI"
BASE COUNT          478 a          159 c          247 g          429 t
ORIGIN      
        1 ttagctgaat gtctcttgat tgaacatagt gacaccttat taattcacgc agcagcagtt
       61 tataatcctt tatctggcca ttcaatttta ctattaggag ataaaggtgc tggaaaaact
      121 acggttgcaa taaggctgtg tatagaacat gggtatcacc ttattggtaa tgaccaagta
      181 atatttggtt cgaactctgg aatactacta acttatgcag ggacatcttt ctttaaaata
      241 agaaggacag ctgttttgtc ggacaattta ctgtttaaat tatttagcaa attttttaac
      301 cgttcattac aatttaataa ggcaagttgg gatgataaaa tttcaatttt cccaaaggag
      361 ttgggaatac gaacttgtaa tttttctact gaaatctcaa aaatttatta tataaagaca
      421 gacaagaaag aaaagcaaat ttatcattca atttggaatg atagtttagc aagtttatat
      481 ctaaatgagc tattgggaag gcatatttct ggacaggttg catgttttca atcagataat
      541 ggaaaatatt tttctacaat gcctttgata aattatgaaa aaaataatcg ggtacgagaa
      601 cagatagtga agggtatttt cgctaaacaa aaattattta aaattacagc agctacgcca
      661 gctaagattg cagaagcgat tatagaaaat gttaagaaaa actcatagtg tgtgtgaatt
      721 tggggattaa tatgaagtat tgaaaaagga gtaagagtaa ttatgtttgt gataggcatt
      781 ccaactttaa atgaggcaga taatatcagt agattagtta aacaaattga tgagtatgct
      841 gtaaatttag ggaaagaaat tattattatt aattcggata gtaaaagtac agatggaact
      901 ccgcagattt ttttagaaac caaaacttat aacaccaaag tttcgatagt atcagaagct
      961 aaaggaaaag gttataatgt gagaaatatt ttcgaatatg caataaatca tgtgccgaat
     1021 ttctccggtt taattttgat tgatggagac gttgtctcaa tgaaaaaaat gtggttagaa
     1081 aagatgttta tagcaattga atctggaaat gatcttataa tcccaaatta tgctagaaaa
     1141 tcttttgaag gaaatgcgac aaaccatttt atttatccca tgttagtgaa atttttcaaa
     1201 cgtgatatgc cttatcaatg catctctggt gattttggat tttcaagagg attgataaaa
     1261 gatttaactt tgaagtgtaa ttggcacaaa tatacattag gttacgggat aga
//
LOCUS       DAUGHY010000120         1273 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.120, whole genome shotgun sequence.
ACCESSION   DAUGHY010000120 DAUGHY010000000
VERSION     DAUGHY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.120"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..229)
                     /locus_tag="VH752_001708"
     CDS             complement(<1..229)
                     /locus_tag="VH752_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ8290032.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHLAIPDEDVEDMVFMGDVLDYLV"
     gene            complement(222..1229)
                     /gene="plsX"
                     /locus_tag="VH752_001709"
     CDS             complement(222..1229)
                     /gene="plsX"
                     /locus_tag="VH752_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEQ8290033.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKADAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDPLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQIINSGGIKTKIGASLLKSSLYEMKKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
BASE COUNT          389 a          286 c          176 g          422 t
ORIGIN      
        1 gaactaaata atctaaaacg tctcccataa ataccatatc ctcaacatct tcatcaggaa
       61 tagcaagatg aaattcatct tcaacattaa taataaattc aactagttca atagagtcaa
      121 ccgctaaatc attttttaaa tgtgtttgtt ctgtaatagc tacactcaaa tagcttcttt
      181 gcttctgaat taagttaatc agcctttcaa aaatctcctg tctagtcatt tacttgagtc
      241 tccttagcaa attcttctac taattgtccg accacgttag tgtctagcat cgtacgtact
      301 tgttttatcg ttgaaaaaat agcttttaca tcactagaac catgagactt tacaacaggt
      361 gcctttaaac caaataaaac tgcacctcca gcactagagt agtcaagagt cttcttcatc
      421 tcgtaaagac tacttttaag tagactagct ccaattttag tcttaatacc gccgctatta
      481 attatctgtt tcagttgccc cataatgcta atagctgttc cttctattga tttcaacact
      541 gcgtttcccg tgaaaccatc tgatacaata acatctgcaa cacctgacat aagttctcgc
      601 gcctcgacat taccaacaaa agatattgta ttatctgctg ttaaaagttc ataagtggcc
      661 ttacgaaggg gatctccttt tgtttcttct gtaccattgt ttaatagacc aacacgtgga
      721 ttagcaatcc cacgtacatt cttagcatag aaagagccta aaatagcata ttgatgtaaa
      781 tgttttgccg tattttcagc attagctcca agatccaaca tatcaaatcc taatccggtt
      841 actgtgggaa tagttgataa taatcctgga cgatcaactc ccttgatacg ccccacaaca
      901 aacaaaccta cagctaaaag agcacctgta ttacctgctg aaataatggc atctgctttt
      961 ccttctttca ctgccttagc tgctaaaacc attgaagcct tctttttacg gcgaacagct
     1021 tttgcaggtt catcatctga cataattttt tcatctgtat gaataatagc aacacgatct
     1081 gattgaataa gataggagtt aatctttgtc tgatcaccgt aaagttgaat ttcgatatct
     1141 gaaaatgctt ctattgcttg attaacaccc tcaacaatag ctttaggagc gttatctcca
     1201 cccatagcat ctattgcaat tctcttcatt tccactcctt taattatatc ttgtgttctt
     1261 acacaatcta tta
//
LOCUS       DAUGHY010000121         1255 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.121, whole genome shotgun sequence.
ACCESSION   DAUGHY010000121 DAUGHY010000000
VERSION     DAUGHY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.121"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..>1255
                     /locus_tag="VH752_001710"
     CDS             <1..>1255
                     /locus_tag="VH752_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEQ8290034.1"
                     /translation="FRNSQIPSTFISAFILIFSLLESGLNTYYQLQGINKEWGFPSRQ
                     IYNSQLKDINNLVNSVSKNSQPFFRMERLLPQTGNDSMKFNYYGISQFSSVRNRLSSS
                     LLDRLGFQSKGTNLNLRYQNNTIIMDSLLGIKYNLSEGPPNKFGFTKLKTSGNTTLYQ
                     NHYSSPLAILTRNVYKDVNLNVNTLDNQTKLLNQLSGKSLTYFNLQPAQLISGANQFN
                     GQISAQASDYQNSVTLNYQINIPKHSQLYVSIPNIIFSNPDAKEMRIQTDNHNFIYTT
                     DNAYSFFDLGYFADAKVATFSFVFPKNKQISFKEPHFYSLSIESYLEAMNSIKQKNVH
                     TYAKSNTVITDYNSKTKGSLIFTLPYDKGWSAQKDGKNLPVKKAQGGFLSVTIPKGKG
                     RVILTFIPNGFKLGLSLSCVGIIAY"
BASE COUNT          440 a          231 c          169 g          415 t
ORIGIN      
        1 cgtttagaaa ttctcaaatc ccatctactt ttatttctgc tttcatactt atctttagcc
       61 ttcttgaatc agggttaaac acctactacc agcttcaagg aattaataag gagtggggat
      121 tcccatcacg acagatatat aatagtcaat taaaggatat taacaacctt gtcaactctg
      181 tgtcaaaaaa tagtcaacct ttttttagaa tggaaaggct acttccccaa acagggaacg
      241 atagcatgaa atttaattat tacggcattt cacaattttc ctctgtaaga aatagactat
      301 ctagttcttt attggatcga ttgggatttc agtctaaagg cacaaattta aaccttagat
      361 accaaaacaa tactattatt atggacagtc tacttggtat aaaatataat cttagcgaag
      421 gacctccaaa taaatttgga tttacaaaac taaaaactag cgggaatact actctttatc
      481 aaaatcacta tagtagccct ttagctatat taacacgtaa tgtttacaaa gatgtcaacc
      541 taaatgtcaa tacccttgat aaccaaacca aattacttaa ccaactaagt gggaaatctt
      601 taacctattt taacttacag ccagctcaac ttatttctgg tgctaatcaa tttaacggac
      661 aaatatctgc acaagcttct gattatcaaa actccgttac ccttaattat caaattaaca
      721 tccctaaaca tagtcaactc tatgttagca tacccaatat tatattttca aatcctgatg
      781 ctaaagagat gcgtattcag acagataatc ataatttcat atatactaca gataacgctt
      841 actctttttt tgatttagga tatttcgccg atgccaaagt tgctacattt tcgtttgttt
      901 ttccaaaaaa taaacaaatt agttttaagg aacctcattt ttatagtttg tctattgaat
      961 cttaccttga agcaatgaat agcattaaac aaaaaaatgt tcatacttac gctaaaagta
     1021 atacggtaat cactgattat aattcaaaaa cgaaaggttc tcttattttt acacttcctt
     1081 acgataaagg ttggtcagca caaaaagatg ggaaaaatct tccagtcaaa aaagcacaag
     1141 gaggatttct atcagttact attcctaaag gaaagggacg tgttatcctt acctttattc
     1201 ctaatggttt taaattaggg ttatctctat cttgtgtagg aattatcgct tatat
//
LOCUS       DAUGHY010000122         1204 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.122, whole genome shotgun sequence.
ACCESSION   DAUGHY010000122 DAUGHY010000000
VERSION     DAUGHY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.122"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..676
                     /locus_tag="VH752_001711"
     CDS             <1..676
                     /locus_tag="VH752_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEQ8290035.1"
                     /translation="EKLIEESIKTSGFFIEDNDVLCIASKVVSVAEGQVMSLNEIQVS
                     DVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNYIGCRLENGLKLTSGGIDRKS
                     VDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSDGREDKRGATQVAIGIYGIHP
                     LRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGTGIPAVLIRGLDYKWSENTSI
                     KEALNE"
     gene            669..>1204
                     /locus_tag="VH752_001712"
     CDS             669..>1204
                     /locus_tag="VH752_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEQ8290036.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFTIEICTPTNHHLTLLKTVITRYVYSYISIEKPLCNRLEDLE
                     EMKSLVESHPHLAKKMFASEQYFFSKILEKLLQVDCFSLDKIKEITVNFSKDRIEDNE
                     NGRFLDKEILGYGIEIPH"
BASE COUNT          440 a          155 c          250 g          359 t
ORIGIN      
        1 agaaaagctt attgaagaaa gcattaaaac ttcaggattt tttatcgaag acaatgatgt
       61 gctttgcata gcttctaaag ttgtatctgt cgcagaaggc caggtgatgt ctttaaacga
      121 gatacaagtg agtgatgtag caaaagaaat ccacaggaat attcctcgta aagatcctag
      181 aataattgaa attatgctaa atttagtcaa tagagatctg tcacgtttag atatcaaaaa
      241 aaattacatt ggttgtcgct tggaaaatgg tttaaagttg acaagtggtg gaatcgatag
      301 aaaatcagta gatgaagtat ttcttttacc taataatccg gatgcttctg caaaaagaat
      361 ttcggaatat cttaaaaaat cacttggaaa aaatgttgca gttgttataa cagatagtga
      421 tggtagagag gataaaagag gtgctactca agtagcaatt ggtatttatg gtattcatcc
      481 tttgagaaaa acagaagtaa tagattcaca aggagagaca ataaaattcc aagaagagac
      541 tttgtgtgat atgattgcag cttgtgctgg cttagtaatg gggcaacgtg gaactggaat
      601 tccagctgtc ctcattaggg gcttggatta taaatggtct gaaaatacta gtattaagga
      661 agcgttaaat gagtagaaat caggactgta agaatattct aatcattggt tttggaaaaa
      721 ttgggaaaat taaagcgtac aagtggctat cacgcggata tgaggtatat gtcaaagatt
      781 tgaaattctc cagcatgaaa taccttaata caaatagtga tgtgttggat gatttatcac
      841 gacaatattt tactattgag atttgtactc cgacaaatca ccaccttacg ctattgaaaa
      901 ccgttataac aaggtatgtc tattcttata ttagcattga aaaaccactg tgtaacaggt
      961 tagaagattt agaagaaatg aagtcgctag tagaaagtca cccacatcta gcaaaaaaaa
     1021 tgtttgccag tgaacaatac ttcttttcga aaattttgga aaaactgtta caagtagatt
     1081 gtttctctct agataaaatc aaggaaataa cagttaattt ttctaaagat aggattgagg
     1141 ataacgagaa tgggagattt ttagataaag agatattggg ctatggaata gagattcctc
     1201 atat
//
LOCUS       DAUGHY010000123         1189 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.123, whole genome shotgun sequence.
ACCESSION   DAUGHY010000123 DAUGHY010000000
VERSION     DAUGHY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.123"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..>1189
                     /locus_tag="VH752_001713"
     CDS             <1..>1189
                     /locus_tag="VH752_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8290037.1"
                     /translation="YFLAVLVITHLLELTLEVQWFITVNEIIESLGILVVVFLINVIT
                     NGLIISKQSLIEFVNFSRKAEKKIKIRKVRAIIAITALLLSYILCLATVFSSTRNMLL
                     SIGMVPVSLLIIVLVVLGTVFTIRYGLAFVVSLLKENKKRLYRPLSNIIYPKFNYRIA
                     TKNKLLTVLGGLLTVTVSVAGMMVMLYAYSLNGIERLTPSAIEYNVESENGQVNVTTI
                     LENDQVSLVDVDLLRLNTNPEVTITESGQTIPYFDIINYSDYKELMKAQGRINSIEGS
                     KSLPLLINYYPTEISLGKTFNLGNAYDVTVKQVSTHNVFSFSTSVTTLVVSDKLYAKL
                     SSCFPEKEMTIRTFNGNSIRSSEAFYNQFSMVPDVISSYSKEYTVKTANIATYIFITF
                     LSIL"
BASE COUNT          394 a          168 c          211 g          416 t
ORIGIN      
        1 tatatttttt agcggtactg gtaattactc atctattgga attaacattg gaagttcagt
       61 ggtttattac agttaatgag attatagagt ctttaggaat attagtcgta gtttttctga
      121 ttaatgtcat cacaaatgga cttatcatta gtaaacagtc tttgattgaa tttgttaatt
      181 tctcaagaaa ggctgagaaa aaaattaaga taagaaaagt cagagctatt attgctatca
      241 ctgcattgct attgtcatat attttatgtt tggcgacagt gttttcatcc acacgaaata
      301 tgctattaag catagggatg gtaccggttt ctctattgat aattgtctta gttgttttag
      361 gaacagtgtt caccatcaga tatggattgg cttttgtagt ttcgttgtta aaagaaaata
      421 aaaaaaggtt ataccgtcct ctgtctaata tcatctatcc caaatttaac tatcgtattg
      481 caacaaaaaa taaattatta acagtcttgg gaggtctttt aacagtaacc gtttcagttg
      541 ccggaatgat ggtaatgctc tatgcttatt ctcttaatgg gatagagagg ttgactccat
      601 ctgccataga atataatgtt gaatcagaaa acggtcaagt caatgttaca actattttag
      661 agaacgacca agtgagcttg gttgatgttg acctattgag attgaacact aacccagaag
      721 taactatcac agaatctggg caaacaattc cttattttga tataattaat tacagtgatt
      781 ataaagagtt aatgaaagct caagggagaa taaattctat tgaaggtagt aagtcactcc
      841 cattgttaat aaattattat ccaacagaaa ttagccttgg aaaaaccttt aacttaggaa
      901 atgcatatga tgttactgta aaacaagtat caacgcataa tgtttttagt ttttctacaa
      961 gtgtcacgac cttggttgtt tctgataaat tatatgctaa acttagttct tgttttccag
     1021 agaaagaaat gacaattagg acttttaatg gaaattcgat taggtcaagt gaagcatttt
     1081 acaatcagtt tagtatggtt cctgatgtta tcagtagtta tagtaaggaa tacacagtaa
     1141 agactgctaa tattgcgact tatatcttta taactttcct atccatact
//
LOCUS       DAUGHY010000124         1167 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.124, whole genome shotgun sequence.
ACCESSION   DAUGHY010000124 DAUGHY010000000
VERSION     DAUGHY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.124"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(361..>1167)
                     /locus_tag="VH752_001714"
     CDS             complement(361..>1167)
                     /locus_tag="VH752_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEQ8290038.1"
                     /translation="VAGRTLTDNDYSNFSRIILLDTVLADDLFGEGNYKSALNKVVSL
                     SDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVASEFGTKEIGSIYIHVNDIQNSMN
                     LGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQFSIMTTVIGSIAAISLLVGGIGV
                     MNIMLVSVTERTREIGLRKALGATRLKILSQFLIESVVLTVLGGLIGLLLAQLSVGAL
                     GNAMTLKGACISLDVALIAVLFSASIGVFFGMLPANKASKLDPIEALRYE"
BASE COUNT          409 a          242 c          159 g          357 t
ORIGIN      
        1 acaatattag caatcgttac aaataataat tgaaaaatac ccgcctttat tacccaagat
       61 aatctgattt ttgtcattaa tgatatcaaa tcagataaaa atgtcattga actaatctcc
      121 tttattttaa acaactcatt gcctgacaac ttcctagccg aaccgaagtt cagctagaaa
      181 taaaagatgt atcacttata cgctatccac atcatcatac caatgatagc tttgctagat
      241 attatcacct caaaaaatgc actaacactt caatgatata actactgtgc taatacaaaa
      301 actagatata atcagacgtt aaactagtca gcgtctatag attaattgat acgaactatt
      361 ctattcataa cgtaatgcct caattggatc taacttacta gctttattag ctggcaacat
      421 tccaaaaaag acacctattg aggcagaaaa taaaactgct attaaggcca catcaagaga
      481 aatacaagct ccttttaatg tcatcgcatt tcctaaagcc cctacactta attgagctaa
      541 taataatcct atcaagcctc caagaactgt tagaacaacg gattcaatca aaaattgtga
      601 taaaattttc aggcgtgttg caccaagtgc tttacgtaaa ccaatttccc tagttcgctc
      661 tgtgacagat accaacataa tattcataac accaattcct cccactaaaa gagagatcgc
      721 tgcaattgaa cctatgactg ttgtcatgat actgaactga ctattgattt cttcaacgat
      781 tttgctatta tcaggaacag cgtactgtcc atctttaata tgcgatatgt tggttagcat
      841 atcagctgct tgatttccta agttcatact gttttgaata tcgttcacat gaatgtagat
      901 agatcctatt tctttggtgc caaactctga agctacctga gtatttgcca tgacagcgcc
      961 gcctgataat ccatcaaaag aaactggcgt ctgatcagtt ttataaacac caataactaa
     1021 ataatcttta tcagataaac ttaccacttt atttaaagct gacttataat taccttcgcc
     1081 aaataggtca tcggctaata ctgtatctaa aagaatgata cgcgaaaaat tagaataatc
     1141 attatctgtt agtgtacgac cagctac
//
LOCUS       DAUGHY010000125         1135 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.125, whole genome shotgun sequence.
ACCESSION   DAUGHY010000125 DAUGHY010000000
VERSION     DAUGHY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.125"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(71..985)
                     /gene="hasC"
                     /locus_tag="VH752_001715"
     CDS             complement(71..985)
                     /gene="hasC"
                     /locus_tag="VH752_001715"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     HasC"
                     /protein_id="HEQ8290039.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILVVTGKAKRSIEDHFDSNFELEYNLQAKGKNELLKLVDETTAINLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNASAKPLTKQLMEDYDKTHASTI
                     AVMKVPHEDVSSYGVIAPQGKAVKGLYSVDTFVEKPQPEDAPSDLAIIGRYLLTPEIF
                     GILERQTPGAGNEVQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALEH
                     PQVKEDLKNYIIKLGKALEKSKVPTHSK"
BASE COUNT          308 a          229 c          229 g          369 t
ORIGIN      
        1 gaacgttgac aattttcaag atataaactt aagttgctat aaacaaggaa aaaagtaaag
       61 taccaacaca ttactttgaa tgtgttggta ctttactttt ttccaaagct tttcctagtt
      121 tgataatgta atttttcaag tcctctttga cctgtgggtg ttctaaggca tagtcgatag
      181 atgttttcat gaatccaaat ttatccccaa catcgtaacg attgccttta aattctcgtg
      241 caaagacacg ctgagtttta ttgagggtat cgatagcatc tgtgagttgc acttcgttac
      301 ctgctccagg ggtctgtctt tccaaaatac caaaaatttc aggggttagg aggtaacgac
      361 caataatagc caaatcacta ggcgcatctt ctggttgtgg tttttcaaca aaggtgtcta
      421 cactgtaaag gcccttgaca gccttgcctt gaggagcgat aaccccatag ctagacacat
      481 cttcatgagg aactttcatc acagcgatag tggatgcatg cgtcttgtca tagtcctcca
      541 tgagttgttt ggtgagaggt ttagcggatg catttgtaat gtccattaag tcatctccaa
      601 gcatgaccac aaagggttca ttgcccacaa aggctttggc ttgtaagaca gcatctccca
      661 gccctcttgg gtggctttga cggataaaat gaaggttaat ggcagtggtt tcatccacta
      721 atttcaacag ttcatttttc cccttagctt ggaggttgta ttctaattca aagtttgaat
      781 caaaatggtc ctcgatagag cgtttagctt ttccggtcac cacaaggatt tcctcgatgc
      841 cagattttag cgcttcttcg acgataaact ggatggttgg tttatcaacg atgggcaaca
      901 tctctttggc aagagctttg gtagcaggta aaaaacgtgt tcctagacct gcagcaggaa
      961 taatggcttt tctgactttg gtcatcagta actcctcact ttttataatc tatggcttag
     1021 tgacattcgt tttcagaacg gaattcattt gacatcatgt tacgttttca tatgattttt
     1081 cgactatttc atagtgctca atcataccac caactgcagt tcttttaaag taaaa
//
LOCUS       DAUGHY010000126         1118 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.126, whole genome shotgun sequence.
ACCESSION   DAUGHY010000126 DAUGHY010000000
VERSION     DAUGHY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.126"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            42..1091
                     /locus_tag="VH752_001716"
     CDS             42..1091
                     /locus_tag="VH752_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEQ8290040.1"
                     /translation="MKAATYLSTGNLQLIDKPKPVIIKPTDAIVQLVKTTICGTDLHI
                     LGGDVPACKEGTILGHEGIGIVKEVGDAVTNFKIGDKVIISCVTSCHTCYYCKRGLSS
                     HCEDGGWILGHLINGTQAEYVHIPHADGSLYHAPDTIDDEALVMLSDILPTSYEIGVL
                     PSHVKPGDNVCIVGAGPVGLAALLTVQFFSPANIIMVDLSQNRLEAAKTFGATHTICS
                     GSSEEVKAIIDDITNGRGVDISMECVGYPATFDICQKIISVGGHIANVGVHGKPVDFN
                     LDELWIKNITLNTGLVNANTTEMLLNVLKTGKIDATRLITHHFKLSEVEKAYETFKHA
                     GANNALKVIIDNDIS"
BASE COUNT          351 a          201 c          223 g          343 t
ORIGIN      
        1 tgaattggtt gattacctct caaccttata aggagttaaa tatgaaagca gcaacttatc
       61 tttcaaccgg aaatctacag ctcatagaca aaccaaaacc agtcattata aagcctacag
      121 atgctatcgt ccagttggta aaaaccacta tttgtggaac tgatttgcat atcttaggtg
      181 gggatgttcc cgcatgtaaa gaaggaacaa tccttggtca tgaaggaatt ggaattgtta
      241 aggaggtcgg tgatgcggtt actaacttta aaatcggaga taaagtgatt atttcttgcg
      301 tcacatcatg tcatacctgt tattattgta aacgaggcct ttcgtcacat tgtgaagatg
      361 gcggttggat tcttggccat ttaattaatg gaacacaagc agaatatgtc catatcccac
      421 atgcagatgg tagtctctat catgctcctg atacgattga tgatgaggca cttgtcatgc
      481 tttctgatat tttaccaact tcatatgaaa ttggtgtctt gccatcacat gtcaagcctg
      541 gagataatgt ttgtattgtt ggtgccggtc cagtaggact cgcagcccta ctaaccgttc
      601 aatttttctc tccagccaat attattatgg tcgacttatc tcaaaatcgc cttgaagcag
      661 ctaaaacatt tggagccaca cacacgattt gttcaggaag tagtgaagaa gtcaaggcta
      721 tcattgacga tataaccaat ggacgtggtg tcgatatttc catggagtgt gttggttatc
      781 ccgcgacatt tgatatttgt caaaaaatta tatcagtagg tgggcatatt gctaacgttg
      841 gggttcatgg gaaaccagtt gatttcaacc tggatgaatt atggattaaa aatatcacct
      901 taaacactgg acttgttaat gccaatacaa ctgaaatgct actaaatgtc ttgaaaactg
      961 gaaagattga cgcaacaaga cttattacac atcattttaa actttcagaa gttgaaaaag
     1021 cttatgaaac ctttaaacat gctggtgcaa ataacgcctt gaaagttatt attgataatg
     1081 atattagcta atcacaaaat aagtagaagc atatttct
//
LOCUS       DAUGHY010000127         1067 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.127, whole genome shotgun sequence.
ACCESSION   DAUGHY010000127 DAUGHY010000000
VERSION     DAUGHY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.127"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..227
                     /locus_tag="VH752_001717"
     CDS             <1..227
                     /locus_tag="VH752_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEQ8290041.1"
                     /translation="TYNLVKSYVDFEKQNQQVVKLKKEYNELSKRTKKEKQLAERLKD
                     DNFVKKYARAKYYLSREGEMIYPIPGLLPK"
     gene            362..>1067
                     /locus_tag="VH752_001718"
     CDS             362..>1067
                     /locus_tag="VH752_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEQ8290042.1"
                     /translation="MRKLLAAMLMTFFLTPLPVISTEKKLIFSKNAVYQLKQDVVQST
                     QFYNQLPSNPNLYQETCAYKDSDLTLPAGRLGVNQPLLIKSLVLNKESLPVFELADGT
                     YVEANRQLIYDDIVLNQVDIDSYFWTQKKLRLYSAPYVLGTQTIPSSFSFAQKVHATQ
                     MAQTNHGTYYLIDDKGWASQEDLVQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTS
                     AGINADKKMYAASISKL"
BASE COUNT          416 a          163 c          168 g          320 t
ORIGIN      
        1 caacttataa tcttgtcaaa agttatgttg attttgaaaa gcaaaatcaa caggtggtta
       61 aattaaaaaa agagtataat gaattgtcaa agaggacaaa aaaagaaaaa caattagcag
      121 aacgactaaa agatgataat tttgtcaaaa aatatgctag ggcaaaatac tatttatcgc
      181 gtgaaggaga aatgatttat cctattccag gactattacc aaaatgatga tggacaatat
      241 tgtaaaaaaa atagaagcat ttcttgcttt ttctgataaa aaattagcag agctgcaaca
      301 ggaaaatcaa aaagttaaag aagaaagtca gtatattcaa aaaaactaga aaggagtcaa
      361 tatgagaaaa ttattagcgg ctatgttaat gacttttttt ctgactcctt taccagtgat
      421 tagtacagaa aaaaaactta tattttcaaa aaatgctgtt tatcaattga aacaagatgt
      481 cgttcaatca acacaattct ataatcagct accctctaat ccaaatcttt atcaagaaac
      541 gtgtgcctat aaagacagtg atttaactct accagcagga agattaggtg taaatcaacc
      601 attacttatt aaatcgcttg ttcttaacaa agaatcttta ccggtttttg agttagctga
      661 tggtacctat gttgaggcta atcgacaatt gatttatgac gatattgtac ttaatcaagt
      721 agatatagat agctattttt ggacacaaaa gaaacttagg ctttattcag ccccttatgt
      781 tttaggtacg caaacaattc cttcttcttt ttcatttgct caaaaagttc atgccactca
      841 aatggcacaa acaaaccatg gaacttatta tcttattgat gataagggct gggcatcaca
      901 agaagatcta gttcaatttg ataaccgcat gttaaaagtc caggaaatgc tcttacaaaa
      961 atataataac ccaaattatt caatttttgt aaagcaactc aacacacaaa caagtgctgg
     1021 tattaatgct gataaaaaaa tgtatgctgc aagtatctcg aagttag
//
LOCUS       DAUGHY010000128         1024 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.128, whole genome shotgun sequence.
ACCESSION   DAUGHY010000128 DAUGHY010000000
VERSION     DAUGHY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.128"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            118..399
                     /locus_tag="VH752_001719"
     CDS             118..399
                     /locus_tag="VH752_001719"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ8290043.1"
                     /translation="MTVLNYVNAGDAKSIHNHLFHYTRQVRRHVKVITIGILSAHYYE
                     ITMLFLQAKIILDCFHIPQRLRQAITRFRIRIINTSAIKPIAYRTLKLY"
     gene            complement(876..963)
                     /locus_tag="VH752_001720"
     tRNA            complement(876..963)
                     /locus_tag="VH752_001720"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(927..929),aa:Ser,seq:gct)
BASE COUNT          344 a          223 c          128 g          329 t
ORIGIN      
        1 tttttagtta cttatccaca ggttaataac tttttttcgc ctaaaatatt atccacaata
       61 gtgacatttt ctattatcag ttatagtata atttcattag gaatctaaaa ccttagcatc
      121 acggttttaa attatgtaaa tgctggggac gcaaaaagca ttcataatca tctctttcat
      181 tataccagac aggtgagaag gcatgtcaaa gttattacca taggcatact cagtgcccac
      241 tactatgaga ttactatgct ttttctacag gctaaaatta tcctcgattg ttttcatatc
      301 ccccaacgtc ttagacaagc tataactcgc tttcgtatcc gaatcataaa tacttctgct
      361 ataaaaccta tcgcgtatag aactctcaaa ctttactgaa aactcataca gcatgattca
      421 cggaagctca tcaataatag ctttattgcc caatttttcg tacgcccata actaacatct
      481 agaaaagaaa agctcttact cacaagatct ctgaatttca ataattattc ctaactctat
      541 caactcttgc tcttcccatt tttcagacaa tttttaaaat ttttctaaaa gaaaagaaaa
      601 caagattagc aatatactta aactcaacta ctctaaggcc aaattagaaa cagtcaacaa
      661 ccttatcaaa gccatcaagt taaacgtttt actcctctac agtagtcaaa gagcctatca
      721 ttttagttat cgaaataatg aacgatttaa atattctatt tagttggttt tgaagattac
      781 aaaaaaaaga cgataaccgt cttctttccc ccttatttat aattcatcct ttaatatgtt
      841 tcatttaaca tattaaccaa aatatcttta caaaacggag aataagggat tcgaaccctt
      901 gcgccagtta cccgacctaa cgatttagca aaccgtcctc ttcagcctct tgagtaattc
      961 tccaaatata tatgggcacg agtggactcg aaccaccgac ctcacgctta tcaggcgtgc
     1021 gctc
//
LOCUS       DAUGHY010000129          970 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.129, whole genome shotgun sequence.
ACCESSION   DAUGHY010000129 DAUGHY010000000
VERSION     DAUGHY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.129"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..>970
                     /locus_tag="VH752_001721"
     CDS             <1..>970
                     /locus_tag="VH752_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ8290044.1"
                     /translation="KLENGLTVYFIKKIGFLEKTAMLTVGFGSLDNKLTVDDESRDAP
                     AGIAHFLEHKLFEDESGGDISLKFTQLGAETNAFTTFNQTSFFFSTASKFQENLELLQ
                     YFVLSANITDESVSREKKIIGQEIDMYQDDADYRAYSGILQNLFPKTSLANDIAGSKA
                     SIQKITKILLETHHTYFYQPTNMSLFIVGDIDIDETFLAIQRFQTTLSYPDRKRVTVD
                     PLHYYPVIKSSSVDMDVTTAKLVVGFRGYLTLTQHSLLTYRIALKLFLSMLIGWTSKI
                     YHTLYEDGKIDDSFDVDVEIHHNFQFVLISLDTPEPIAMSNYIRQKL"
BASE COUNT          326 a          161 c          159 g          324 t
ORIGIN      
        1 aaaattagaa aatggtttaa ctgtttattt tataaaaaaa atagggtttt tagaaaaaac
       61 agctatgttg acagtaggtt tcggttctct tgacaataag ttgacagtcg atgacgaatc
      121 acgagatgct ccagcaggta ttgcacactt tttagaacat aaactctttg aagatgaatc
      181 aggtggagat atttcattaa agtttacaca gctaggagca gagacaaatg cctttacaac
      241 tttcaaccaa acgagtttct tcttttcaac agctagtaaa tttcaggaaa atttggaact
      301 ccttcaatat tttgttcttt ctgctaatat aacagatgaa tctgttagta gagaaaaaaa
      361 aataatcggg caagaaattg atatgtatca agatgatgct gactaccgtg catatagtgg
      421 aatccttcag aacttatttc caaaaacaag tttagctaat gatattgctg gctcaaaagc
      481 atccattcaa aagatcacga aaatattatt ggaaacgcat catacttatt tttaccaacc
      541 tactaatatg agtctattca ttgtcggtga tattgatatt gacgaaactt ttttagctat
      601 tcaaaggttt caaaccacat tatcctatcc agatagaaaa agggtaactg ttgatccttt
      661 acactactat cctgtcatta aatcatcttc tgttgacatg gatgttacta ctgccaagct
      721 ggttgttggt tttcgagggt atctgacctt aactcagcat tcactattaa cgtatcgtat
      781 tgccttgaag ctatttttat caatgcttat aggttggact tctaaaatct atcatacgct
      841 atatgaagat ggtaaaatag acgattcatt tgatgtggat gttgaaattc atcataattt
      901 tcaatttgta ttaatatcac ttgatacacc agagcctatc gcaatgtcta actatatccg
      961 tcaaaaatta
//
LOCUS       DAUGHY010000130          960 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.130, whole genome shotgun sequence.
ACCESSION   DAUGHY010000130 DAUGHY010000000
VERSION     DAUGHY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.130"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            102..>960
                     /locus_tag="VH752_001722"
     CDS             102..>960
                     /locus_tag="VH752_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ8290045.1"
                     /translation="MKIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKVFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQIDIFILGDFDDYRVVQLIHQFPL
                     DNRNKNLNFFHLQNSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFI"
BASE COUNT          338 a          156 c          149 g          317 t
ORIGIN      
        1 cactagaatc ccgacaatta agtcaaaaaa agttatcact cacctaacct gaataaaaat
       61 ggtataatcg taaggatcac actatttatt aaggaaacat tatgaaaatt gttcagggag
      121 tacaacttca tctgattaag accaaacagt ttaaaaccaa tcatattacc tttcgttttt
      181 caggtgattt gaaccaaaaa acagtagcaa aaagagtctt agtggcacag atgttagcaa
      241 cagctaatga gtgttatcca actgttaggc agtttagaga aaaattagca cgactttatg
      301 gagctagctt gtcaactaac gtgttaacaa aagggttagt tcatattgtg gatatcgata
      361 ttacttttat tcaagataga tatgcttgca atggggaaaa aatactagat gaaatgattc
      421 agtttttaaa agatatcttg ttctcacccc tattatccat cgcccagtat cagcctaaag
      481 tttttgagac tgaaaaaaat aatttgatta actacatcga gtcagataga gaggattctt
      541 tttactacag ctctcttaag gtaaaagaat tgttttattg taataagaat ctacaaatgt
      601 cagaatatgg tagcccagaa ctaattgcaa aagagactgc ctatactagc tatcaagaat
      661 ttcataaaat gctcaatgaa gatcagattg atattttcat tttaggagat tttgatgatt
      721 atcgcgttgt tcagctaatt catcaatttc cacttgataa ccgaaataaa aacctaaatt
      781 tctttcattt acaaaattct gtcaatatta ttaaagaatc tattgaaaaa agagcagttc
      841 atcaatctat tttacaatta gcttatcact tcccttcagt ttttggacaa cgtgactatt
      901 atgctttagt tcttttaaat gggttgttag gctcttttgc acattcccgt ctctttataa
//
LOCUS       DAUGHY010000131          914 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.131, whole genome shotgun sequence.
ACCESSION   DAUGHY010000131 DAUGHY010000000
VERSION     DAUGHY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.131"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..>914
                     /locus_tag="VH752_001723"
     CDS             <1..>914
                     /locus_tag="VH752_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEQ8290046.1"
                     /translation="LFVVSTLLIVFLPKKLEVATIIFILVFGTISAIISPINDIPDEY
                     VHYSRTVYISEGDINLTNNNKKLRISKDVDKLIKQSGKTFITSNLKATKHSTREYSYP
                     YIKGTNAYYSFSYIPQALGILVGNALDLPILLTYYFGRLCNLISYAMLAFIAIKLSGS
                     FKQVIAVVTLLPMNIYLAASFNQDGFAIGLVLVTIGLFINLLSSKDKSNYNTKFFLYL
                     VLCGLLVLSKFTYFLLVCLPLFIPNEKFGKNTKLVILKKLGGLLLIFLFAAMWFRLYG
                     QVKTPYVADFLKEVNVSQQVKNMLESPI"
BASE COUNT          299 a          144 c          138 g          333 t
ORIGIN      
        1 tctttttgtg gtgagcactt tactgatagt gtttttacca aaaaagttag aggttgctac
       61 gatcattttt attttagtat ttggaacgat atctgctatc atatcaccta taaatgatat
      121 tcctgatgaa tatgttcatt attcaaggac agtttatatc tcagaaggtg atattaatct
      181 taccaacaat aacaaaaagt taagaatatc caaagatgtt gataagctta ttaaacagtc
      241 aggaaaaaca tttataacat caaacttgaa agcaactaaa cacagcacaa gagagtatag
      301 ctatccttac atcaaaggaa ctaatgcata ttatagtttt tcttatatac cacaagcttt
      361 gggtatttta gtaggaaatg cgctggattt acctatactt ttaacatatt atttcggaag
      421 attgtgcaac cttattagct acgctatgct tgcttttata gctatcaagt tgtctggttc
      481 ttttaaacaa gtgatagctg ttgttacttt actacctatg aacatttatt tagcagcatc
      541 ttttaatcaa gacggctttg ctatcggttt agttttagta acaattggat tgtttattaa
      601 cttgctctca tcaaaagata aatctaacta taataccaaa ttcttccttt atttagttct
      661 atgtgggtta ctagttcttt caaaattcac atacttttta ttagtatgcc ttccattatt
      721 tattcctaat gaaaaattcg gaaaaaatac caagttagtc atcttgaaaa aattgggagg
      781 actattactc atttttcttt ttgctgcaat gtggtttaga ctttatggac aggttaaaac
      841 cccatatgtc gcggattttc taaaagaagt aaacgttagc cagcaagtta aaaatatgct
      901 agaatcccct atag
//
LOCUS       DAUGHY010000132          898 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.132, whole genome shotgun sequence.
ACCESSION   DAUGHY010000132 DAUGHY010000000
VERSION     DAUGHY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.132"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            92..571
                     /gene="rlmH"
                     /locus_tag="VH752_001724"
     CDS             92..571
                     /gene="rlmH"
                     /locus_tag="VH752_001724"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEQ8290047.1"
                     /translation="MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTP
                     DKASFADNQLIMSKEAQRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYST
                     ITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            complement(728..801)
                     /locus_tag="VH752_001725"
     tRNA            complement(728..801)
                     /locus_tag="VH752_001725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(765..767),aa:Arg,seq:tct)
BASE COUNT          332 a          128 c          153 g          285 t
ORIGIN      
        1 cctttgtaca aaggttttta ttagtagttg aagaaaaata acttgtggat aaattgtgta
       61 aaaagtaatg ttcttgtgat acaattaagc tatgaaagtg aaactaattt gtgttggaaa
      121 actaaaagaa agatacctca aagatggtat ttcagaatat caaaaaagac tttcccgttt
      181 ttgtcaattc gaaatgattg agttaactga tgaaagaaca ccagataaag caagttttgc
      241 tgataatcaa ttgattatga gtaaagaagc tcagcgaatc cataaaaaaa ttggggaacg
      301 tgattttgta attgcattag ctattgaagg aaaacagttt ccttcagaga catttagtga
      361 attaatatca ggggttactg ttaagggata ttcaactatc acgtttatta ttggtgggag
      421 tcttggatta gattcaatta ttaaaaaaag agctaatatg ctaatgagtt ttggattgtt
      481 aaccttacct catcagttaa tgcgtctagt ccttactgag caaatttatc gtgcttttat
      541 gattacacaa ggcagtcctt atcataagta agagagtgtg aactggaata actaactctt
      601 accactttat tagataatag ttcggtataa ttaaaagcaa atattaaaaa aagacgtact
      661 aaatcctatc ttttcaatac actaaggtaa atacaaaaaa agcactaaaa cagtgctttt
      721 ttatctttga tcccagcagg attcgaacct gcgaccgttc gcttagaagg cgaatgctct
      781 atccagctga gctatgagac cttaaccaca tcattttaac aaaaattatc taaaactgtc
      841 aatatttatt ttgaaaaaac tagaaaataa cattatatcc cgcgttataa aatgaagt
//
LOCUS       DAUGHY010000133          883 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.133, whole genome shotgun sequence.
ACCESSION   DAUGHY010000133 DAUGHY010000000
VERSION     DAUGHY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.133"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            40..312
                     /gene="rpsP"
                     /locus_tag="VH752_001726"
     CDS             40..312
                     /gene="rpsP"
                     /locus_tag="VH752_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEQ8290048.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            322..561
                     /locus_tag="VH752_001727"
     CDS             322..561
                     /locus_tag="VH752_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEQ8290049.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDEK"
BASE COUNT          283 a          148 c          156 g          296 t
ORIGIN      
        1 tttcttatac attactaaaa gaaaagagac tatttaaaaa tggcagtaaa aatccgttta
       61 actcgtatgg gttctaagaa aaaacctttc taccgtatta atgttgcaga ttcacgtgca
      121 ccacgtgatg gtcgttttat cgaaacagtt ggaacataca acccattagt ggctgaaaat
      181 caaattacta ttaaagagga tcgtgttctt gagtggttat ctaaaggagc gcagccttct
      241 gataccgttc gtaacatctt gtctaaagct ggtgttatgg ctaagttcca cgaccaaaaa
      301 ttctctaaat aataacagcc tatggatacc attgaaaatc ttattatcgc tattgtgaaa
      361 cctttgattt cacaaccaga taatcttacc attaaaattg aagatactcc tgacttttta
      421 gagtatcatc ttgacttgga tgctcaagac atcggtcgtg ttatcggcaa aaaaggtcga
      481 actattactg cgataagatc gattgtctac tcggttccaa cactaggaaa aaaagttcgc
      541 ttagttattg atgagaaata atcactaact tgagatggat tgaaaagctt gttgacttta
      601 agtcaacaag ctttttgaat agcaaaaatc ttaatatttc tgaaatgaaa agattagatt
      661 tttctgtctt taactaaaaa gaagttaaag aatggcttag ctgattgcct ttataagaga
      721 ttatactggt ttttgatagc tgctgtcatg taaaatagct acaattcatc ttacgagatt
      781 gaccttagca ttgccagtgc agtctttatt ttccaacctg ctgtttgttg tatgtttata
      841 gtttcctaaa aaatgttctc tcagtttttt gggggacatt cct
//
LOCUS       DAUGHY010000134          875 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.134, whole genome shotgun sequence.
ACCESSION   DAUGHY010000134 DAUGHY010000000
VERSION     DAUGHY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.134"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            182..875
                     /locus_tag="VH752_001728"
                     /pseudo
     CDS             182..875
                     /locus_tag="VH752_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984809.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic (srt) production protein"
BASE COUNT          332 a          125 c          183 g          235 t
ORIGIN      
        1 ttcaagatta ctttaaaaat aaggaagtcg gtgtttcaga tcgattagaa agtctacaga
       61 aacagtacat gcttttcaag aagaacgaga taaagaggag gtatccactg aagagagtaa
      121 ggaagtcttc aagaaataca aggggcaacg agatgccatc catgagtttg aagttgaact
      181 tgtggaacat caaattgaga ggttaattgc tgaggacatt tatattaagg tgtcttttgg
      241 tgtaaagcag agtggtctta ttttcattcc taactatcaa ttggatatta ggaaagaaga
      301 aaatcataaa aaatataaag tctatattcg tgagaccgca caattcttca tttacaacaa
      361 ggaggcgtct aaacttaatc gctatatgcg agggcgtgag ctcatttacc agttaaccaa
      421 tgatagcaag agtattccaa aaagaagacg accaaaacca ttgatacact taagaaaaag
      481 attgaagaaa taagtctcct cattgagtta gatacggaga ataagcctta tcaagatatt
      541 aaagatgaca ttgttaaaga tatggctcaa ttagatttga ccattacaga gttgcaggac
      601 catattgctc atctcaataa ggttgcggag gtcttgctta atatgaacaa caacgatata
      661 gagaaccgcc gtttggccag atatgactat gccaagatga acttgactgc agctataaaa
      721 atagaagaag ttgagaaaga gattgaaact tctcaaaatg aacttaatat atccatagat
      781 gagtatgaat atctagtaag aaggttggaa aagtttggag agatcttgag ttatagcaaa
      841 attatcgata cttctcgaaa tgaaatacaa tggga
//
LOCUS       DAUGHY010000135          847 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.135, whole genome shotgun sequence.
ACCESSION   DAUGHY010000135 DAUGHY010000000
VERSION     DAUGHY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.135"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            51..824
                     /gene="cfa"
                     /locus_tag="VH752_001729"
     CDS             51..824
                     /gene="cfa"
                     /locus_tag="VH752_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HEQ8290050.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTHSVLADDASNPDTILMNNNQA
                     NLQRDALVQKLDEGHQQLEAIKHEAKGTDIEATVNKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKA
                     QVQEIKALEEKVINYPDLQPTDRATIYTKAKLNKAIWNTRLERDKKVLGIKPFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQDALETALKS"
BASE COUNT          295 a          147 c          149 g          256 t
ORIGIN      
        1 taacttcact taactattta aataggttta ttatattcag gaggaataat atgaaaccca
       61 aacaccttct ctgtctttca acagttgttg ctggattagc tttattttca actatgacgc
      121 actcagtttt agctgatgat gcttcaaatc ccgatactat tttaatgaat aacaaccaag
      181 cgaatcttca aagagatgct ctagtgcaaa agcttgatga gggacatcaa caattagaag
      241 ctattaaaca tgaagctaaa ggtactgata ttgaggctac tgttaacaaa gctatcgatg
      301 ctgttgatca catgaagagt tctatacgct tcaatactga aacgatctat gattttagtt
      361 caattggggc aagagtagaa gcattatcag atgctatcaa agcaatcgta ttttccacaa
      421 cccaattaac tcataaagta gaaaaagcgc atactgatat ggggtttgct attactaaat
      481 tagtcattcg tattatagat ccatttgcat ctgttgacgc tatcaaagct caagtacaag
      541 aaattaaagc acttgaggaa aaagttatca attatcctga tttacagcca acagaccgtg
      601 ctactatcta tactaaagct aaactaaata aagctatttg gaacactcgt ttagaaagag
      661 ataaaaaggt tttggggatc aaaccatttg atgtttacaa tagacttaac aaagctatca
      721 cacatgcggt tggtgtccaa ctaaatccga caaccactgt tcaacaagtt gacgatgaag
      781 ttattgctgt gcaagatgct ttggaaacag ctcttaagtc atagagtgta taagtaatgt
      841 gttgtat
//
LOCUS       DAUGHY010000136          827 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.136, whole genome shotgun sequence.
ACCESSION   DAUGHY010000136 DAUGHY010000000
VERSION     DAUGHY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.136"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(1..459)
                     /locus_tag="VH752_001730"
                     /pseudo
     CDS             complement(1..459)
                     /locus_tag="VH752_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
BASE COUNT          260 a          196 c          137 g          234 t
ORIGIN      
        1 cggattatct tgcaagagaa tagcggagct accgagatta ctttgatgag accaaaggac
       61 aatctcacgc ctagcagagt cgcttaaatg agacccatgc accatactaa aaatttccca
      121 atcatcacta agagcctgag caaaattccc acgaatggtt ccgcaaagag catccgtagg
      181 attggttact ctcatcatgg ttcaaaaaga caaaaccacc ttagtcccca agaagtgtct
      241 caattaaaac aggaccactt gtcatgtaag ctttaagttt aggatcaaaa ggtttatcac
      301 caaggcctcg tcgtgcttag ccaagtattt cgaactagct tgtcttagct cgaatcgctt
      361 aaacgtaaat cttaggcgtt caatccaccg caaaacctct ccaactacct ccccttgctt
      421 aaccccatct ggcttaatca taaaaaatgt ttgttccatc acactactct tcttttgcct
      481 attataacat gaaaacgctt taacagaaag aatagaaagg tatccgactg ctggtattaa
      541 ccctctttct caagttatca tcggcaatgt tgaatggaga ccattcaaaa cagcatagct
      601 ctaaaacgtc aaactcaatt ttttgtctag ctctattgtt ttgggaccat tcaaaacagc
      661 atagctctaa aacataacct aagcgttgga tgttgtggtc aatcgttttg ggaccattca
      721 aaacagcata gctctaaaac ctcgtagact atttttgtct aaaaaatttc gtaatcgcac
      781 tatttgtctc agctagactt cagtcttgaa aagcccctgt attactg
//
LOCUS       DAUGHY010000137          779 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.137, whole genome shotgun sequence.
ACCESSION   DAUGHY010000137 DAUGHY010000000
VERSION     DAUGHY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.137"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            55..>779
                     /gene="mf2"
                     /locus_tag="VH752_001731"
     CDS             55..>779
                     /gene="mf2"
                     /locus_tag="VH752_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNase Mf2"
                     /protein_id="HEQ8290051.1"
                     /translation="MKLSKQKASLLTAVLLLLSLSITTITVDAARVRTYPNVSHANTH
                     YKNTVSSKLLPFTANYQLQLGELDNLNRATFSHIQLQDRHETKDVRTKINYDPVGWHN
                     YQFPYGDGSKSSWVMNRGHLVGYQFCGLNDEPRNLVAMTAWLNTGAYSGANDSNPEGM
                     LYYENRLDSWLALHPDFWLDYKVTPIYSGNEVVPRQIELQYVGIDSSGELLTIRLNSN
                     KESIDENGVTTVILENSAPNINL"
BASE COUNT          258 a          142 c          144 g          235 t
ORIGIN      
        1 gtgcaatatt tgatccactc taaaagctaa aaatggaaag gtttaggtgt tatcatgaaa
       61 ttatctaaac aaaaggcaag tttgcttacc gctgttttac tgttactttc tctttctata
      121 acgacaataa ctgttgacgc agctagggta cgaacttatc cgaatgtatc ccatgcaaac
      181 acacattata aaaatactgt ttctagtaag ctcttacctt ttactgctaa ttatcagtta
      241 caacttggtg agttggataa ccttaaccgc gctacctttt cacatatcca actgcaagat
      301 agacacgaaa ctaaagatgt acgcactaaa ataaattatg accctgtggg atggcacaac
      361 tatcaatttc catacggaga tggctcaaaa tcttcatggg taatgaatcg tgggcatcta
      421 gtcggatatc aattttgtgg attaaacgat gaaccaagaa atttagtggc aatgacagcc
      481 tggctaaaca caggtgctta ttcaggagca aatgacagca atcctgaagg gatgttatac
      541 tatgaaaacc gtttagattc ttggcttgca cttcaccctg acttttggct agattataaa
      601 gtcactccta tatatagtgg caatgaagta gtgcctcgac aaattgaatt acagtatgtt
      661 ggaattgatt catctggtga gttgcttact ataaggctaa atagtaataa agaaagtatt
      721 gatgaaaatg gtgttactac agtaatctta gaaaactctg ctccaaatat caaccttga
//
LOCUS       DAUGHY010000138          666 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.138, whole genome shotgun sequence.
ACCESSION   DAUGHY010000138 DAUGHY010000000
VERSION     DAUGHY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.138"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..>666)
                     /locus_tag="VH752_001732"
     CDS             complement(<1..>666)
                     /locus_tag="VH752_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8290052.1"
                     /translation="YVDNAKTYDSIAALYPQQLNHIRKRSVDYVKVSSIQTSKGMTSA
                     DYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIHLTGVSPSLLVFGKLIGAMLATT
                     VQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAIGIGLGISIKNEAFLTVASN
                     TIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDSLFYLIFGGQYNPIPVTLIV
                     NIS"
BASE COUNT          246 a          117 c          108 g          195 t
ORIGIN      
        1 tactaatatt aacgatcaaa gttacaggaa tcggattata ttgaccacca aaaataagat
       61 agaacaaact atcattaacc catttaatag gagatatatt cgacaattga ttaataatag
      121 agctgtgtaa tgttgtcaat ggaacgtaac tgcctcctaa aaaggcgaat ataggaataa
      181 tagtattgga tgcaaccgtt aaaaaagcct catttttaat gcttatgcct agcccgatac
      241 ctatagctac agagagatag actaaggagg ctgttatacc aataagcatc cactcattag
      301 tgccccaatt aactcgtaaa acaaaccttg taaaaatgta taaaagactc aattgaacag
      361 ttgttgctaa catagcacct attaattttc caaagaccaa aagacttgga gaaactcctg
      421 ttaagtgaat acgatttgta atccgttgtt gacgatctga caaaacaagg ttcatcgcag
      481 acatcatact ataaaaagta atcatggtaa acatggaaat agcataataa tcagctgatg
      541 tcattccttt acttgtctgt attgaactga ccttaacata gtccacactt cgctttctga
      601 tatgatttaa ttgttgagga taaagagctg ctatcgagtc ataagtttta gcattatcta
      661 cataat
//
LOCUS       DAUGHY010000139          634 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.139, whole genome shotgun sequence.
ACCESSION   DAUGHY010000139 DAUGHY010000000
VERSION     DAUGHY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.139"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(172..510)
                     /locus_tag="VH752_001733"
     CDS             complement(172..510)
                     /locus_tag="VH752_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8290053.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
BASE COUNT          216 a          123 c           88 g          207 t
ORIGIN      
        1 cccaaaatgt agcaaaaaat aaacagaaag ttaaattaaa gcttgaatgc ttaagttcta
       61 tgtgatatag aatacaccta atagtagcaa aaaaatacct aacagatttt tatctgttag
      121 gtatttaaac cttatcccct caaagacact aaaatgccat tagaagcgtt tttattttat
      181 ttgttcaaaa cgcaagtgaa ccacaatctt atcaccatag ccattgcgct ctaaatctcg
      241 ctgaagacga taagaaatgt aatgttctgc aatggtaata tatccagcat ccatcaaacg
      301 attctccaca agggattgga ccatattgat agtaggacgt tctacatgtg cttcttctaa
      361 atcaataact acttttttag tgacatgtgt taagttttgt cgccacgtgt catcaataac
      421 atagaccgtc cttgctgctt taataattgc ttgataaatt ttatctggat caaaatcagc
      481 aacgtggcca tcacgcttaa taatttgcat aagatcatct cttttcataa gttttcatct
      541 ctacttacat tatgcaaatg ttattagcat ttgtcaagtc atagcactca tacccgaaaa
      601 actactttct tattttgaaa cacagtaatc actg
//
LOCUS       DAUGHY010000140          621 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.140, whole genome shotgun sequence.
ACCESSION   DAUGHY010000140 DAUGHY010000000
VERSION     DAUGHY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.140"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..395)
                     /locus_tag="VH752_001734"
     CDS             complement(<1..395)
                     /locus_tag="VH752_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ8290054.1"
                     /translation="MHNEKSNRTLVSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKV
                     KPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYL
                     KDLLRRQESQKRQFHKLAYEEIGEVAHAIP"
     gene            complement(391..464)
                     /locus_tag="VH752_001735"
     tRNA            complement(391..464)
                     /locus_tag="VH752_001735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(428..430),aa:Arg,seq:acg)
     gene            complement(544..617)
                     /locus_tag="VH752_001736"
     tRNA            complement(544..617)
                     /locus_tag="VH752_001736"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(581..583),aa:Asn,seq:gtt)
BASE COUNT          181 a          148 c           94 g          198 t
ORIGIN      
        1 ggaatggcat gtgcaacctc ccctatctct tcatatgcta acttatggaa ctgacgcttt
       61 tgactttctt gacggcgtaa taaatctttc aaataagatg aaaattttgt tttaaaatag
      121 cgatataaac gctcttcttc ctcaattaac tctgggtacc tttctagtaa ctgtaataag
      181 ataatatgtc cttcttgtaa ccagtcatct ctatcccaca attgaatata atagtgtcgc
      241 tttaatttca aaataatggg cttaactttt tcaaacgctg ctcttgtctc tatcgacatt
      301 ttgagtctcc ttttcttttt aactatccct agtataaaga gaaccacaaa aaaacaacag
      361 gacactaatg tcctgttgct tttttcatta tgcaccctag aggagtcgaa cctctaaccg
      421 cctgattcgt agtcaggtac tctatccagt tgagctaagg gtgccaacgc atatcttgcc
      481 tctatcttac cacactcttc tctaaaatgc aaaaaaagat aggacgcttc ctacctctct
      541 ctactccgcc agtaggactc gaacctacga catcatgatt aacagtcatg cgctactacc
      601 aactgagcta tggcggataa a
//
LOCUS       DAUGHY010000141          603 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.141, whole genome shotgun sequence.
ACCESSION   DAUGHY010000141 DAUGHY010000000
VERSION     DAUGHY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.141"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..344
                     /locus_tag="VH752_001737"
     CDS             <1..344
                     /locus_tag="VH752_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEQ8290055.1"
                     /translation="YGDEENLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQIHFEI
                     WNNGERFSDSTLKKGDKLFYTEDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPARGG
                     ASAIISIKKKI"
     gene            501..>603
                     /locus_tag="VH752_001738"
     CDS             501..>603
                     /locus_tag="VH752_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic streptin"
                     /protein_id="HEQ8290056.1"
                     /translation="MNNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGS"
BASE COUNT          220 a           80 c          120 g          183 t
ORIGIN      
        1 tttatggaga tgaggaaaat ctgataagag ctctttctaa ccttttagtt aatgcaataa
       61 gatttatgcc cgtattagat aaaaaaatcg aagttatact gtctgagtca ggagagcaaa
      121 ttcattttga aatatggaat aatggtgagc gtttcagtga tagtacactg aaaaagggag
      181 ataaattatt ttacacggaa gattatagca gaggtaacaa acattatgga atcggtttgg
      241 catttgttaa aggcgttgct atcaaacacg gtgggaatct tcaattgaat aatccagcta
      301 gaggaggtgc tagcgccata atctcgataa aaaagaaaat ttaatagatt tgattgaaac
      361 tggagtattt caggcaaaaa atcgtaaatt tttgtgattt ttggctaaaa tacttttttt
      421 ctgaacaaat tctgaacttg gggtgtcata ataatcatgt aaatatatcg gatgcaaaaa
      481 cgtaagagag gaactagaaa atgaataaca caattaaaga ctttgatctc gatttgaaaa
      541 caaataaaaa agacactgct acaccttatg ttggtagccg ttacctatgt acccctggtt
      601 ctt
//
LOCUS       DAUGHY010000142          579 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.142, whole genome shotgun sequence.
ACCESSION   DAUGHY010000142 DAUGHY010000000
VERSION     DAUGHY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.142"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..>579
                     /locus_tag="VH752_001739"
     CDS             <1..>579
                     /locus_tag="VH752_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEQ8290057.1"
                     /translation="KKTKLIFSFTSIFIAIISRPVFGLEVDNNSLLRNIYSTIVYEYS
                     DTVIDFKTSHNLVTKKLDVRDARDFFINSEMDEYAANDFKAGDKIAVFSVPFDWNYLS
                     KGRVTAYTYGGITPYQKTSIPKNIPVNLWINGKQISVPYNEISTNKTTVTAQEIDLKV
                     RKFLISQHQLYSSGSSYKSGKLVFHTNDNSDKY"
BASE COUNT          218 a           90 c           82 g          189 t
ORIGIN      
        1 aaaaaaacaa aacttatttt ttcttttact tcaatattca ttgcaataat ttctcgtcct
       61 gtgtttggat tagaagtaga taataattcc cttctaagga atatctatag tacgattgta
      121 tatgaatatt cagatacagt aattgatttt aaaaccagtc ataacttagt gactaagaaa
      181 cttgatgtta gagatgctag agattttttt attaactccg aaatggacga atatgcagcc
      241 aatgatttta aagctggaga taaaatagct gtgttctccg tcccatttga ttggaactat
      301 ttatcaaaag gaagagtcac agcatatacg tacggcggaa taacacccta ccaaaaaact
      361 tcaataccta aaaatatccc tgttaattta tggattaatg gaaagcagat ctctgttcct
      421 tacaacgaaa tatcaactaa caaaacaaca gttacagctc aagaaattga tctaaaggtt
      481 agaaaatttt taatatcaca acatcaatta tattcttctg gttctagcta caaaagtggt
      541 aaattagttt ttcatacaaa tgataattca gataaatat
//
LOCUS       DAUGHY010000143          534 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.143, whole genome shotgun sequence.
ACCESSION   DAUGHY010000143 DAUGHY010000000
VERSION     DAUGHY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.143"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..>534)
                     /locus_tag="VH752_001740"
     CDS             complement(<1..>534)
                     /locus_tag="VH752_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEQ8290058.1"
                     /translation="YAIELMTTLGEVALGSQTIFNILFMPAFVMNLLILFFRPYITQM
                     AIALIRGQIKEFNKIQVQLFAYLGVFSLIALVGSGLFGIPFLSMLYGTNLTDYWVDFM
                     LIMLGGSIGSFATVIDNILTAMRKQQLLLIPYTGGFLISLLITNLFVMKYHILGAALS
                     FLITMLVWLGLSIMIYL"
BASE COUNT          221 a           97 c           70 g          146 t
ORIGIN      
        1 atagataaat cataatggat aatcccaacc aaaccaacat tgttattaaa aagctcaaag
       61 cagctcctaa aatgtgatat ttcataacaa aaagattggt aattaataac gaaatcaaaa
      121 atcctcctgt atatggaata agaagaagtt gctgttttct catagcggtt aagatattat
      181 cgataactgt cgcaaaacta ccaatcgagc ctcctagcat aatcaacata aagtctaccc
      241 aataatccgt aaggttagta ccatatagca ttgataaaaa gggaatacca aacaaaccac
      301 ttccaactaa cgctatcagt gaaaaaactc ctaaataagc aaacaattga acttgtatct
      361 tattaaactc ttttatttga cctctgatta aagcaatagc catctgtgta atataaggtc
      421 taaaaaataa aattagcaaa ttcataacaa aggctggcat aaataaaata ttaaaaattg
      481 tttgagagcc caaagcaact tcacctaaag tcgtcattag ttcaatagcg tatt
//
LOCUS       DAUGHY010000144          508 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.144, whole genome shotgun sequence.
ACCESSION   DAUGHY010000144 DAUGHY010000000
VERSION     DAUGHY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.144"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..>508
                     /locus_tag="VH752_001741"
     CDS             <1..>508
                     /locus_tag="VH752_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEQ8290059.1"
                     /translation="SNILKINKKATIVIKSTVPIGFTEYLRNRFHYNDIIFSPEFLRE
                     GSTIHDQLYPSRTIVGNESRNSQLFLDILTDISVEKDSPSLLVGSSEAEAIKLFSNAY
                     LAQKIAFFNELDTFAEMQNLDSKKIIEAMGYDQRIGNSHNNPSFGFGGYCLPKDIKQL
                     EYHFKEIPA"
BASE COUNT          177 a           76 c           89 g          166 t
ORIGIN      
        1 agtaatattt taaagataaa caaaaaggct acaattgtaa taaagtcgac agttccaatt
       61 ggttttacag aatatttaag gaatcgattt cactacaacg atatcatttt ttcacctgag
      121 ttccttaggg aaggatcaac tattcatgat caattgtatc cttcgagaac tatagttgga
      181 aatgaatcta gaaattctca attattctta gacatactaa cagatatatc ggttgaaaaa
      241 gactcgccat ctttattagt tggctcttct gaagcagaag cgataaagtt attttcgaat
      301 gcatacttgg cacaaaaaat tgcttttttt aatgagttgg atacgtttgc tgaaatgcaa
      361 aatttggact caaaaaaaat tattgaggct atgggatatg accagagaat aggaaattcg
      421 cacaataatc cttctttcgg ttttggtggg tactgtcttc ctaaggatat taagcaatta
      481 gagtatcatt ttaaagaaat tccagcac
//
LOCUS       DAUGHY010000145          498 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.145, whole genome shotgun sequence.
ACCESSION   DAUGHY010000145 DAUGHY010000000
VERSION     DAUGHY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.145"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..488)
                     /locus_tag="VH752_001742"
     CDS             complement(<1..488)
                     /locus_tag="VH752_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEQ8290060.1"
                     /translation="MLWQKIIKILGIIALIGTFFLAFWLYRLGILNDSNALKDLVQRY
                     RLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIIIGSIVLFLLV
                     KTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVMITGLTDMSL
                     KR"
BASE COUNT          192 a           98 c           74 g          134 t
ORIGIN      
        1 aagcgcttta aagacatgtc cgtcaatccc gtaatcataa ccataatatc agctggagaa
       61 acaggcgatg ccatacaaaa aataaataat ttttcatatc ctggtgtctc caaacgcctt
      121 tcatacttgt aaaatgtttt gtcatttaca aagagtaaga taaatttacg accataagtc
      181 ttaacaagca agaagaggac tatactgcca ataatgatgc ctacataatt ataaataaaa
      241 cctgttacag ggccaaagat taaaaaacct gcaactgtcg taaggcctcc aggaatcaca
      301 ggaaaaacaa tctgtatgat ctgcacgaca ataaaaacaa acggccccca taatctatag
      361 cgctgcacta agtctttaag agcattacta tcgtttaata tacctagtct atacagccaa
      421 aaagcgagga aaaaagtacc aattagtgca ataattccta aaattttgat aattttttgc
      481 cataatatat aggctttt
//
LOCUS       DAUGHY010000146          482 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.146, whole genome shotgun sequence.
ACCESSION   DAUGHY010000146 DAUGHY010000000
VERSION     DAUGHY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.146"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(30..102)
                     /locus_tag="VH752_001743"
     tRNA            complement(30..102)
                     /locus_tag="VH752_001743"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67..69),aa:Thr,seq:tgt)
     gene            complement(113..194)
                     /locus_tag="VH752_001744"
     tRNA            complement(113..194)
                     /locus_tag="VH752_001744"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Leu,seq:tag)
     gene            complement(200..272)
                     /locus_tag="VH752_001745"
     tRNA            complement(200..272)
                     /locus_tag="VH752_001745"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(237..239),aa:Lys,seq:ttt)
     gene            complement(304..376)
                     /locus_tag="VH752_001746"
     tRNA            complement(304..376)
                     /locus_tag="VH752_001746"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(341..343),aa:Asp,seq:gtc)
     gene            complement(394..466)
                     /locus_tag="VH752_001747"
     tRNA            complement(394..466)
                     /locus_tag="VH752_001747"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(431..433),aa:Val,seq:tac)
BASE COUNT          125 a          139 c          101 g          117 t
ORIGIN      
        1 cactgaacta cgttcgcaac taatctcaat gccggctaca tgacttgaac acgcgaccct
       61 ctgattacaa atcagatgct ctaccaactg agctaagccg gctaatcttt tatgcgggtt
      121 aagggacttg aacccccacg ccgttaagcg ccagatccta aatctggtgc gtctgccaat
      181 tccgccaaac ccgcttttat gacccgtact gggctcgaac cagtgaccca ttgattaaaa
      241 gtcaattgct ctaccaactg agctaacgag tctttccttc aaaaaatacg actactcttt
      301 gaacggtccc gacgggaatc gaacccgcga tcttcgccgt gacagggcga cgtgataacc
      361 gctacactac gggacctgta ttttccaata ctatgggagt taacgggatc gaaccgctga
      421 ccctctgctt gtaaggcaga tgctctccca gctgagctaa actcccattg agtgtgtcat
      481 ta
//
LOCUS       DAUGHY010000147          477 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.147, whole genome shotgun sequence.
ACCESSION   DAUGHY010000147 DAUGHY010000000
VERSION     DAUGHY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.147"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
BASE COUNT          176 a           91 c           56 g          154 t
ORIGIN      
        1 aaaaccatag ttaaatatac tagatatgat acctcaactc gaaaagataa caccttgata
       61 tgctcaacaa tagactcttc taatggttag tgtaacacaa aacactcgtt gtttgacact
      121 ctataaattc actgccaagc tatctaaacg taccacagta ctgatataca aaaatcttat
      181 caagagtcaa ctcctgataa gataatcaca aatgatttcg ttttgaactt gtttagacgt
      241 atatccttga caaggatatc aaatacattt aaatacagta gatatccgca aaaagtatta
      301 aacatatcag ctcttctgac ctatgccgat atttttcttt gactattcag tatttactat
      361 agtttatact attttatccc atacttgtga acttacttgt aaacaggaca gtcgccagat
      421 aacacatgat aataactgta aatcaaaaaa tgattacact tttttcatag atttacc
//
LOCUS       DAUGHY010000148          475 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.148, whole genome shotgun sequence.
ACCESSION   DAUGHY010000148 DAUGHY010000000
VERSION     DAUGHY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.148"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..>475
                     /locus_tag="VH752_001748"
     CDS             <1..>475
                     /locus_tag="VH752_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEQ8290061.1"
                     /translation="YGDYDPLGSGKISKIADNKDYRVEDLLKAVAQQSDNVATNILGY
                     YLCHQYDKAFRSEIKALSGIDWDMEQRLLTSRSAANMMEAIYHQKGQIISYLSNTEFD
                     QQRITKNITVPVAHKIGDAYDYKHDVAIVYGNTPFILSIFTNKSTYEDITAIADDV"
BASE COUNT          162 a           73 c           82 g          158 t
ORIGIN      
        1 tttatggaga ctatgatcca ttgggaagtg gtaaaattag taaaatagct gataataaag
       61 attatcgtgt tgaagaccta ctgaaagctg tagcacaaca atcggataat gtagcaacta
      121 atattttagg ttattatcta tgtcatcagt atgataaagc tttccgctca gagataaaag
      181 ctttatcagg tatcgattgg gatatggagc agcgcttatt aacttctcgt tcagctgcaa
      241 atatgatgga agctatttat catcaaaaag gccaaattat ttcttacctt tcaaataccg
      301 aatttgatca acaacgtatc acaaaaaata ttactgttcc agttgcacat aaaattggtg
      361 atgcttatga ttataaacat gacgttgcta ttgtttacgg taatactcca tttattttgt
      421 ctatttttac aaataaatca acctatgaag atattacggc tattgcagat gacgt
//
LOCUS       DAUGHY010000149          468 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.149, whole genome shotgun sequence.
ACCESSION   DAUGHY010000149 DAUGHY010000000
VERSION     DAUGHY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.149"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..>468)
                     /locus_tag="VH752_001749"
     CDS             complement(<1..>468)
                     /locus_tag="VH752_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ8290062.1"
                     /translation="IPKGKVYALIGPNGAGKSTIMKILTGLVSKTSGSIIFEGREWSR
                     RDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVSESAILPLLNKVGLGNIDKRPVKQ
                     FSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIGIQELREIIESFKSEGMTIM"
BASE COUNT          143 a           94 c           63 g          168 t
ORIGIN      
        1 cataattgtc attccttctg atttaaatga ctctataatt tcccttaatt cttgaattcc
       61 aattgggtcc aagccattag taggttcgtc taatataagt aatttaggtg aatttattaa
      121 agagatagct atacctaacc gttgcttcat tccaagagaa aattgtttta ctggtctctt
      181 gtcaatattt cctagaccaa ctttatttaa taatggaagt atagcgcttt ctgaaacacc
      241 aagcattgtt gtaactacct tcatattatc gtaagcactc aaatttttat aaagtggtgg
      301 ttcttcaata atactcccga tttttcgcag atcccgacgt gaccattctc taccttcaaa
      361 aataatagaa ccacttgtct tactaactaa gcctgtcaaa attttcataa tagttgattt
      421 tccagcacca tttggaccga ttaaagcata cacttttccc ttaggaat
//
LOCUS       DAUGHY010000150          468 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.150, whole genome shotgun sequence.
ACCESSION   DAUGHY010000150 DAUGHY010000000
VERSION     DAUGHY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.150"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..354
                     /locus_tag="VH752_001750"
     CDS             <1..354
                     /locus_tag="VH752_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ8290063.1"
                     /translation="FNLYEQLEKYPYQLSGGQRQRVSIIRAIIKEPEIIFADEPTGAL
                     DLKSSEGTMRILSEINKTEKVTILMVTHDVLSASYADRVVLLKDGKLHMEIDKEDCGE
                     TFYDVISQALSDRGE"
BASE COUNT          160 a           62 c           88 g          158 t
ORIGIN      
        1 tttaatttat atgagcagtt ggaaaaatat ccgtatcaac tttctggagg tcaaagacag
       61 agggtcagta ttatcagagc aatcattaaa gaaccagaga ttatttttgc tgacgaacca
      121 actggagcac ttgacttaaa atcttctgaa ggaacaatga ggattttgag tgaaatcaat
      181 aaaacagaaa aggtaactat tttaatggtt actcatgacg ttttatcagc ttcttatgcg
      241 gatagagttg tacttctcaa agatggtaaa cttcacatgg agattgataa agaggattgt
      301 ggagagacgt tttatgatgt aattagtcaa gccttatccg atagaggaga gtaatatgat
      361 ttggtccatt acaaaatcta acatcaaaaa aaacttttcg ttatatcgta tctattttct
      421 agctacgatt ggtttattaa gtatttttat agcttttcta aattttat
//
LOCUS       DAUGHY010000151          434 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.151, whole genome shotgun sequence.
ACCESSION   DAUGHY010000151 DAUGHY010000000
VERSION     DAUGHY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.151"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            117..341
                     /locus_tag="VH752_001751"
     CDS             117..341
                     /locus_tag="VH752_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8290064.1"
                     /translation="MTYFRLKKELNWSLRSKGIVVYEGNQKLQIAINTGKNNSEKVFF
                     DTFFEVGRAAATFSTAKNICFGLGLLRSQK"
BASE COUNT          143 a           57 c           88 g          146 t
ORIGIN      
        1 aataaacaaa taggagggca attgtttaac tggttaatta gaatttttgt tattttcagg
       61 aagatactca gggctttttt ataagttgat gtattaacaa aagataaaga ggtttcatga
      121 cttattttag attaaaaaaa gaattaaact ggtcactaag aagtaaagga attgtagttt
      181 acgaagggaa tcaaaaactt cagatagcta ttaatacagg taaaaataac agtgaaaaag
      241 tcttttttga tacttttttt gaggtgggtc gggcggcagc gactttctcc acagcaaaaa
      301 acatctgttt tggtcttggc ttacttcgtt cgcagaaata ggaaatcggg tatcacatta
      361 gcagagtagt tcatgctttt tgagcatcac actgggtctg tctacgttct aaaagctttt
      421 gtcatttttg tatc
//
LOCUS       DAUGHY010000152          427 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.152, whole genome shotgun sequence.
ACCESSION   DAUGHY010000152 DAUGHY010000000
VERSION     DAUGHY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.152"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(64..137)
                     /locus_tag="VH752_001752"
     tRNA            complement(64..137)
                     /locus_tag="VH752_001752"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(101..103),aa:Met,seq:cat)
     gene            complement(147..236)
                     /locus_tag="VH752_001753"
     tRNA            complement(147..236)
                     /locus_tag="VH752_001753"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ser,seq:tga)
     gene            complement(259..332)
                     /locus_tag="VH752_001754"
     tRNA            complement(259..332)
                     /locus_tag="VH752_001754"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(296..298),aa:Ile,seq:cat)
     gene            complement(353..426)
                     /locus_tag="VH752_001755"
     tRNA            complement(353..426)
                     /locus_tag="VH752_001755"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(390..392),aa:Met,seq:cat)
BASE COUNT          114 a          120 c           98 g           95 t
ORIGIN      
        1 atggagcttc aatccattgc tctaccaact gagctaccga gccaacaact acatacttat
       61 gtattgcggg agcaggattt gaacctacga ccttcgggtt atgagcccga cgagctacct
      121 aactgctcca tcccgcgata gttaaaagga ggatgtggga ttcgaaccca cgcacgcttt
      181 tacacgcctg acggttttca agaccgttcc cttcagccgg acttgggtaa tcctccaaaa
      241 taatgattat ctatgatatg gaccttgtag gactcgaacc tacgaccgct cggttatgag
      301 ccgagtgctc taaccagttg agctaaaggt cccaagtcat ccataaaaat aatagcggcg
      361 aaggggatcg aacccccgac ctcccgggta tgaaccggac gctctagcca gctgagctac
      421 accgcca
//
LOCUS       DAUGHY010000153          416 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.153, whole genome shotgun sequence.
ACCESSION   DAUGHY010000153 DAUGHY010000000
VERSION     DAUGHY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.153"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            <1..409
                     /locus_tag="VH752_001756"
     CDS             <1..409
                     /locus_tag="VH752_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEQ8290065.1"
                     /translation="GTPGTAVALFKMCQLIDLNFIDDINESLIAQMRSSDKNVYSLTM
                     CHGLAGVTFIYDLIGKNYSSERLTRFAEELRQQIITSFSTTKVFGYTDREKFGEIEVE
                     LESIGILQGVSGIVLFLLNPYSDEELLWQRIFI"
BASE COUNT          140 a           53 c           88 g          135 t
ORIGIN      
        1 cggtacgcct ggaactgcag ttgccctttt taaaatgtgt caattgatag atttaaattt
       61 tattgacgat attaatgaaa gtttaattgc tcagatgaga agttcagaca aaaatgtcta
      121 ttctcttaca atgtgccatg gtttggcagg agtaactttt atatatgact tgattggtaa
      181 aaattattca agtgagaggc taactcgctt tgcagaagag cttcgccaac aaataattac
      241 atcgttttca actacaaaag tatttgggta tactgacaga gaaaaatttg gagaaataga
      301 agttgaactg gagtcaatag ggatattgca aggggtatca gggatagtgt tattcttatt
      361 aaatccttac agtgacgagg agctattgtg gcaaagaata tttatttaat ttgaaa
//
LOCUS       DAUGHY010000154          408 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.154, whole genome shotgun sequence.
ACCESSION   DAUGHY010000154 DAUGHY010000000
VERSION     DAUGHY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.154"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
BASE COUNT          125 a           77 c           67 g          139 t
ORIGIN      
        1 aactagctct ctcaaaaaag attaaaaata aaggcattct aaagaatcat ttaagaaaaa
       61 tttaaggttg ctatcgtaga atgaaaacat cctaaaaagc ggtttaccta aaccgttggg
      121 agaataggtt ttccccttgt tttccctttt cataaatctc ctgggcacct atcttcttag
      181 gtgccctccc ttgtttaaac ctgaccagtt aaccggctgg ttaggttttt aaatttaaaa
      241 agtcaacagc aaaaatatga tatcacccgc gtgttcttag ctctctcaat tatgggctaa
      301 aaacacgcat caatcaatct attgatgaag tttgctgctt tttgatgatc tttattgtga
      361 ctatactggt aatcctattg attttagttg acttttacgg tagattcg
//
LOCUS       DAUGHY010000155          405 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.155, whole genome shotgun sequence.
ACCESSION   DAUGHY010000155 DAUGHY010000000
VERSION     DAUGHY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.155"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            complement(<1..>405)
                     /locus_tag="VH752_001757"
     CDS             complement(<1..>405)
                     /locus_tag="VH752_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8290066.1"
                     /translation="PNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFI
                     VKPFSLKQFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFL
                     TYREYEILELLSRHPYKVFTKEEIYEQVYSDEA"
BASE COUNT          121 a           85 c           53 g          146 t
ORIGIN      
        1 agcttcatcg ctatatactt gttcatatat ctcttctttt gtgaaaactt tatacggatg
       61 acgtgacagt aattctaaaa tttcgtactc tctataagtc aggaataacg gctgtttatc
      121 aatatataac atacgttcct ctagatagat ttctataggt ggaaaactgc gcttcactct
      181 ctcctcagcc tcattcttta tctttgctct ctcttctctc ttcaaatgag agttaacttt
      241 agctacgaat tgttttaagc taaatggttt cacaataaaa tcgtctcccc ctatatttaa
      301 agcttggaca atttcattat cgccatctaa agcgctaaca aaaataattg gagaatgaac
      361 ttcttcccga attttataac aaaactcagt tccacttata ttagg
//
LOCUS       DAUGHY010000156          380 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain AUSMDU00080335
            SAMN33608999-rid17956973.denovo.156, whole genome shotgun sequence.
ACCESSION   DAUGHY010000156 DAUGHY010000000
VERSION     DAUGHY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33608999
            Sequence Read Archive: SRR23751857
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001723582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001723582.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,719
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 21 of 42
            Pseudo Genes (incomplete)         :: 20 of 42
            Pseudo Genes (internal stop)      :: 13 of 42
            Pseudo Genes (multiple problems)  :: 10 of 42
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33608999-rid17956973.denovo.156"
                     /strain="AUSMDU00080335"
                     /serotype="EMM12.0"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST36"
     gene            1..380
                     /gene="speh"
                     /locus_tag="VH752_001758"
                     /pseudo
     CDS             1..380
                     /gene="speh"
                     /locus_tag="VH752_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922229.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeH"
BASE COUNT          153 a           44 c           75 g          108 t
ORIGIN      
        1 tataagcatg attctaactt gattgaagcc gatagtataa aaaattctcc agatattgta
       61 acaagccata tgttgaaata tagtgtcaag gataaaaatt tgtcagtttt tttgagaaag
      121 attggatatc acaggaattc aaagataaag aagtagatat ttatgctcta tctgcacaag
      181 aggtttgtga atgtccaggg aaaaggtatg aagcgtttgg tggaattaca ttaactaatt
      241 cagaaaaaaa agaaattaaa gttcctataa acgtgtggga taaaagtaaa caacagccgc
      301 ctatgtttat tacagtcaat aaaccgaaag taaccgctca ggaagtggat ataaaagtta
      361 gaaagttatt gattaagaaa
//
